LOCUS       DAJTRD010000001       136733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTRD010000001 DAJTRD010000000
VERSION     DAJTRD010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 136733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 136733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..136733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.001"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            165..1598
                     /gene="cscA"
                     /locus_tag="OO315_000001"
     CDS             165..1598
                     /gene="cscA"
                     /locus_tag="OO315_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5889083.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1606..2664)
                     /locus_tag="OO315_000002"
     CDS             complement(1606..2664)
                     /locus_tag="OO315_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5889084.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2832..3050
                     /locus_tag="OO315_000003"
                     /pseudo
     CDS             2832..3050
                     /locus_tag="OO315_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3068..4396
                     /gene="dsdA"
                     /locus_tag="OO315_000004"
     CDS             3068..4396
                     /gene="dsdA"
                     /locus_tag="OO315_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5889085.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4504..6042)
                     /gene="emrY"
                     /locus_tag="OO315_000005"
     CDS             complement(4504..6042)
                     /gene="emrY"
                     /locus_tag="OO315_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5889086.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6042..7205)
                     /gene="emrK"
                     /locus_tag="OO315_000006"
     CDS             complement(6042..7205)
                     /gene="emrK"
                     /locus_tag="OO315_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5889087.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7621..8235
                     /gene="evgA"
                     /locus_tag="OO315_000007"
     CDS             7621..8235
                     /gene="evgA"
                     /locus_tag="OO315_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5889088.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8240..11833
                     /gene="evgS"
                     /locus_tag="OO315_000008"
     CDS             8240..11833
                     /gene="evgS"
                     /locus_tag="OO315_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5889089.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11889..13034)
                     /gene="yfdE"
                     /locus_tag="OO315_000009"
     CDS             complement(11889..13034)
                     /gene="yfdE"
                     /locus_tag="OO315_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5889090.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13108..14052)
                     /gene="yfdV"
                     /locus_tag="OO315_000010"
     CDS             complement(13108..14052)
                     /gene="yfdV"
                     /locus_tag="OO315_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5889091.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14122..15816)
                     /gene="oxc"
                     /locus_tag="OO315_000011"
     CDS             complement(14122..15816)
                     /gene="oxc"
                     /locus_tag="OO315_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5889092.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15870..17120)
                     /gene="frc"
                     /locus_tag="OO315_000012"
     CDS             complement(15870..17120)
                     /gene="frc"
                     /locus_tag="OO315_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5889093.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17633..18256)
                     /locus_tag="OO315_000013"
     CDS             complement(17633..18256)
                     /locus_tag="OO315_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5889094.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18553..18828
                     /locus_tag="OO315_000014"
     CDS             18553..18828
                     /locus_tag="OO315_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889095.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18905..19147)
                     /locus_tag="OO315_000015"
     CDS             complement(18905..19147)
                     /locus_tag="OO315_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5889096.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19500..20420
                     /gene="lpxP"
                     /locus_tag="OO315_000016"
     CDS             19500..20420
                     /gene="lpxP"
                     /locus_tag="OO315_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5889097.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20775..20846
                     /gene="ypdK"
                     /locus_tag="OO315_000017"
     CDS             20775..20846
                     /gene="ypdK"
                     /locus_tag="OO315_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5889098.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20911..22149)
                     /gene="alaC"
                     /locus_tag="OO315_000018"
     CDS             complement(20911..22149)
                     /gene="alaC"
                     /locus_tag="OO315_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5889099.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22526..24012
                     /locus_tag="OO315_000019"
                     /pseudo
     CDS             22526..24012
                     /locus_tag="OO315_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24027..24761
                     /gene="ypdB"
                     /locus_tag="OO315_000020"
     CDS             24027..24761
                     /gene="ypdB"
                     /locus_tag="OO315_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5889100.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24774..25631
                     /locus_tag="OO315_000021"
                     /pseudo
     CDS             24774..25631
                     /locus_tag="OO315_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25634..28129)
                     /gene="ptsP"
                     /locus_tag="OO315_000022"
     CDS             complement(25634..28129)
                     /gene="ptsP"
                     /locus_tag="OO315_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5889101.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28154..29191)
                     /gene="ypdE"
                     /locus_tag="OO315_000023"
     CDS             complement(28154..29191)
                     /gene="ypdE"
                     /locus_tag="OO315_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5889102.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29191..30274)
                     /gene="ypdF"
                     /locus_tag="OO315_000024"
                     /pseudo
     CDS             complement(29191..30274)
                     /gene="ypdF"
                     /locus_tag="OO315_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30289..31536)
                     /locus_tag="OO315_000025"
     CDS             complement(30289..31536)
                     /locus_tag="OO315_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5889103.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31558..31884)
                     /locus_tag="OO315_000026"
     CDS             complement(31558..31884)
                     /locus_tag="OO315_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5889104.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32103..33068)
                     /gene="glk"
                     /locus_tag="OO315_000027"
     CDS             complement(32103..33068)
                     /gene="glk"
                     /locus_tag="OO315_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5889105.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33272..34528
                     /locus_tag="OO315_000028"
     CDS             33272..34528
                     /locus_tag="OO315_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5889106.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34643..34969
                     /locus_tag="OO315_000029"
     CDS             34643..34969
                     /locus_tag="OO315_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5889107.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35110..36348)
                     /locus_tag="OO315_000030"
     CDS             complement(35110..36348)
                     /locus_tag="OO315_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5889108.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36684..37886
                     /gene="nupC"
                     /locus_tag="OO315_000031"
     CDS             36684..37886
                     /gene="nupC"
                     /locus_tag="OO315_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5889109.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(37936..40048)
                     /locus_tag="OO315_000032"
                     /pseudo
     CDS             complement(37936..40048)
                     /locus_tag="OO315_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40256..40331)
                     /locus_tag="OO315_000033"
     tRNA            complement(40256..40331)
                     /locus_tag="OO315_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40296..40298),aa:Ala,seq:ggc)
     gene            complement(40371..40446)
                     /locus_tag="OO315_000034"
     tRNA            complement(40371..40446)
                     /locus_tag="OO315_000034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40411..40413),aa:Ala,seq:ggc)
     gene            40667..41026
                     /locus_tag="OO315_000035"
     CDS             40667..41026
                     /locus_tag="OO315_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5889110.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            41028..41420
                     /locus_tag="OO315_000036"
     CDS             41028..41420
                     /locus_tag="OO315_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5889111.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41472..42887)
                     /gene="gltX"
                     /locus_tag="OO315_000037"
     CDS             complement(41472..42887)
                     /gene="gltX"
                     /locus_tag="OO315_000037"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5889112.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43146..43221
                     /locus_tag="OO315_000038"
     tRNA            43146..43221
                     /locus_tag="OO315_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43179..43181,aa:Val,seq:tac)
     gene            43266..43341
                     /locus_tag="OO315_000039"
     tRNA            43266..43341
                     /locus_tag="OO315_000039"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43299..43301,aa:Val,seq:tac)
     gene            43346..43421
                     /locus_tag="OO315_000040"
     tRNA            43346..43421
                     /locus_tag="OO315_000040"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43379..43381,aa:Lys,seq:ttt)
     gene            43452..43577
                     /locus_tag="OO315_000041"
     ncRNA           43452..43577
                     /ncRNA_class="other"
                     /locus_tag="OO315_000041"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43697..44038
                     /locus_tag="OO315_000042"
     CDS             43697..44038
                     /locus_tag="OO315_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5889113.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(44029..44955)
                     /locus_tag="OO315_000043"
     CDS             complement(44029..44955)
                     /locus_tag="OO315_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5889114.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            45045..46043
                     /locus_tag="OO315_000044"
     CDS             45045..46043
                     /locus_tag="OO315_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5889115.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(46040..46258)
                     /locus_tag="OO315_000045"
     CDS             complement(46040..46258)
                     /locus_tag="OO315_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5889116.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46260..48275)
                     /gene="ligA"
                     /locus_tag="OO315_000046"
     CDS             complement(46260..48275)
                     /gene="ligA"
                     /locus_tag="OO315_000046"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5889117.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48346..49332)
                     /gene="zipA"
                     /locus_tag="OO315_000047"
     CDS             complement(48346..49332)
                     /gene="zipA"
                     /locus_tag="OO315_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5889118.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49562..50323
                     /gene="cysZ"
                     /locus_tag="OO315_000048"
     CDS             49562..50323
                     /gene="cysZ"
                     /locus_tag="OO315_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5889119.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50508..51479
                     /gene="cysK"
                     /locus_tag="OO315_000049"
     CDS             50508..51479
                     /gene="cysK"
                     /locus_tag="OO315_000049"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5889120.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51863..52120
                     /gene="ptsH"
                     /locus_tag="OO315_000050"
     CDS             51863..52120
                     /gene="ptsH"
                     /locus_tag="OO315_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5889121.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52165..53892
                     /gene="ptsI"
                     /locus_tag="OO315_000051"
     CDS             52165..53892
                     /gene="ptsI"
                     /locus_tag="OO315_000051"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5889122.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53933..54442
                     /gene="crr"
                     /locus_tag="OO315_000052"
     CDS             53933..54442
                     /gene="crr"
                     /locus_tag="OO315_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5889123.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54484..55335)
                     /gene="pdxK"
                     /locus_tag="OO315_000053"
                     /gene_synonym="thiJ"
     CDS             complement(54484..55335)
                     /gene="pdxK"
                     /locus_tag="OO315_000053"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5889124.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55440..55814
                     /locus_tag="OO315_000054"
     CDS             55440..55814
                     /locus_tag="OO315_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5889125.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55847..56570
                     /locus_tag="OO315_000055"
                     /pseudo
     CDS             55847..56570
                     /locus_tag="OO315_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56774..57685)
                     /gene="cysM"
                     /locus_tag="OO315_000056"
     CDS             complement(56774..57685)
                     /gene="cysM"
                     /locus_tag="OO315_000056"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5889126.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57819..58916)
                     /gene="cysA"
                     /locus_tag="OO315_000057"
     CDS             complement(57819..58916)
                     /gene="cysA"
                     /locus_tag="OO315_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5889127.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58906..59781)
                     /gene="cysW"
                     /locus_tag="OO315_000058"
     CDS             complement(58906..59781)
                     /gene="cysW"
                     /locus_tag="OO315_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5889128.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59781..60614)
                     /gene="cysT"
                     /locus_tag="OO315_000059"
     CDS             complement(59781..60614)
                     /gene="cysT"
                     /locus_tag="OO315_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5889129.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60614..61630)
                     /gene="cysP"
                     /locus_tag="OO315_000060"
     CDS             complement(60614..61630)
                     /gene="cysP"
                     /locus_tag="OO315_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5889130.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61934..62725)
                     /gene="ucpA"
                     /locus_tag="OO315_000061"
     CDS             complement(61934..62725)
                     /gene="ucpA"
                     /locus_tag="OO315_000061"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5889131.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62912..63712)
                     /gene="murR"
                     /locus_tag="OO315_000062"
     CDS             complement(62912..63712)
                     /gene="murR"
                     /locus_tag="OO315_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5889132.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63876..64772
                     /gene="murQ"
                     /locus_tag="OO315_000063"
     CDS             63876..64772
                     /gene="murQ"
                     /locus_tag="OO315_000063"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5889133.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64776..66200
                     /gene="murP"
                     /locus_tag="OO315_000064"
     CDS             64776..66200
                     /gene="murP"
                     /locus_tag="OO315_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5889134.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66380..67279)
                     /gene="yfeX"
                     /locus_tag="OO315_000065"
     CDS             complement(66380..67279)
                     /gene="yfeX"
                     /locus_tag="OO315_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5889135.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67375..67950)
                     /locus_tag="OO315_000066"
     CDS             complement(67375..67950)
                     /locus_tag="OO315_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5889136.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(68011..68460)
                     /locus_tag="OO315_000067"
     CDS             complement(68011..68460)
                     /locus_tag="OO315_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5889137.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68447..68872)
                     /locus_tag="OO315_000068"
     CDS             complement(68447..68872)
                     /locus_tag="OO315_000068"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5889138.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69086..69955
                     /gene="amiA"
                     /locus_tag="OO315_000069"
     CDS             69086..69955
                     /gene="amiA"
                     /locus_tag="OO315_000069"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5889139.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69959..70858
                     /gene="hemF"
                     /locus_tag="OO315_000070"
     CDS             69959..70858
                     /gene="hemF"
                     /locus_tag="OO315_000070"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5889140.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70864..71916)
                     /gene="eutR"
                     /locus_tag="OO315_000071"
                     /pseudo
     CDS             complement(70864..71916)
                     /gene="eutR"
                     /locus_tag="OO315_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(71962..72462)
                     /gene="eutK"
                     /locus_tag="OO315_000072"
     CDS             complement(71962..72462)
                     /gene="eutK"
                     /locus_tag="OO315_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5889141.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72475..73134)
                     /gene="eutL"
                     /locus_tag="OO315_000073"
     CDS             complement(72475..73134)
                     /gene="eutL"
                     /locus_tag="OO315_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5889142.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74316..75107
                     /locus_tag="OO315_000074"
     CDS             74316..75107
                     /locus_tag="OO315_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889143.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75827..76222
                     /locus_tag="OO315_000075"
     CDS             75827..76222
                     /locus_tag="OO315_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5889144.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76240..76503
                     /locus_tag="OO315_000076"
     CDS             76240..76503
                     /locus_tag="OO315_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889145.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76526..77068
                     /locus_tag="OO315_000077"
     CDS             76526..77068
                     /locus_tag="OO315_000077"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889146.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77207..77494
                     /locus_tag="OO315_000078"
     CDS             77207..77494
                     /locus_tag="OO315_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889147.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77533..77739
                     /locus_tag="OO315_000079"
     CDS             77533..77739
                     /locus_tag="OO315_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889148.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78051..78536
                     /locus_tag="OO315_000080"
     CDS             78051..78536
                     /locus_tag="OO315_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5889149.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78517..78795
                     /locus_tag="OO315_000081"
     CDS             78517..78795
                     /locus_tag="OO315_000081"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889150.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78788..80248
                     /locus_tag="OO315_000082"
     CDS             78788..80248
                     /locus_tag="OO315_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5889151.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80282..81091
                     /gene="murI"
                     /locus_tag="OO315_000083"
     CDS             80282..81091
                     /gene="murI"
                     /locus_tag="OO315_000083"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5889152.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81210..81904)
                     /locus_tag="OO315_000084"
     CDS             complement(join(81210..81658,81658..81904))
                     /locus_tag="OO315_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5889153.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82423..83796)
                     /gene="eutA"
                     /locus_tag="OO315_000085"
     CDS             complement(82423..83796)
                     /gene="eutA"
                     /locus_tag="OO315_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5889154.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83771..84250)
                     /gene="eutP"
                     /locus_tag="OO315_000086"
     CDS             complement(83771..84250)
                     /gene="eutP"
                     /locus_tag="OO315_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5889155.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84263..84598)
                     /gene="eutS"
                     /locus_tag="OO315_000087"
     CDS             complement(84263..84598)
                     /gene="eutS"
                     /locus_tag="OO315_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5889156.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84891..87170)
                     /gene="maeB"
                     /locus_tag="OO315_000088"
     CDS             complement(84891..87170)
                     /gene="maeB"
                     /locus_tag="OO315_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5889157.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            87459..88409
                     /gene="tal"
                     /locus_tag="OO315_000089"
     CDS             87459..88409
                     /gene="tal"
                     /locus_tag="OO315_000089"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5889158.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            88429..90432
                     /gene="tkt"
                     /locus_tag="OO315_000090"
     CDS             88429..90432
                     /gene="tkt"
                     /locus_tag="OO315_000090"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5889159.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90535..91578)
                     /locus_tag="OO315_000091"
     CDS             complement(90535..91578)
                     /locus_tag="OO315_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5889160.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91704..92279)
                     /gene="nudK"
                     /locus_tag="OO315_000092"
     CDS             complement(91704..92279)
                     /gene="nudK"
                     /locus_tag="OO315_000092"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5889161.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(92347..94326)
                     /gene="aegA"
                     /locus_tag="OO315_000093"
     CDS             complement(92347..94326)
                     /gene="aegA"
                     /locus_tag="OO315_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5889162.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            94532..96249
                     /gene="narQ"
                     /locus_tag="OO315_000094"
                     /pseudo
     CDS             94532..96249
                     /gene="narQ"
                     /locus_tag="OO315_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            96451..99564
                     /gene="acrD"
                     /locus_tag="OO315_000095"
     CDS             96451..99564
                     /gene="acrD"
                     /locus_tag="OO315_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5889163.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(99663..99722)
                     /gene="ypfM"
                     /locus_tag="OO315_000096"
     CDS             complement(99663..99722)
                     /gene="ypfM"
                     /locus_tag="OO315_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5889164.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            100103..100459
                     /locus_tag="OO315_000097"
     CDS             100103..100459
                     /locus_tag="OO315_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5889165.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            100463..101590
                     /gene="dapE"
                     /locus_tag="OO315_000098"
     CDS             100463..101590
                     /gene="dapE"
                     /locus_tag="OO315_000098"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5889166.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            101618..101818
                     /locus_tag="OO315_000099"
     CDS             101618..101818
                     /locus_tag="OO315_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5889167.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(101928..102626)
                     /gene="ypfH"
                     /locus_tag="OO315_000100"
     CDS             complement(101928..102626)
                     /gene="ypfH"
                     /locus_tag="OO315_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5889168.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(102700..104715)
                     /gene="tmcA"
                     /locus_tag="OO315_000101"
     CDS             complement(102700..104715)
                     /gene="tmcA"
                     /locus_tag="OO315_000101"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5889169.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(104730..105593)
                     /locus_tag="OO315_000102"
     CDS             complement(104730..105593)
                     /locus_tag="OO315_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5889170.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(105761..106474)
                     /gene="purC"
                     /locus_tag="OO315_000103"
     CDS             complement(105761..106474)
                     /gene="purC"
                     /locus_tag="OO315_000103"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5889171.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(106687..107721)
                     /gene="bamC"
                     /locus_tag="OO315_000104"
     CDS             complement(106687..107721)
                     /gene="bamC"
                     /locus_tag="OO315_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5889172.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(107738..108616)
                     /gene="dapA"
                     /locus_tag="OO315_000105"
     CDS             complement(107738..108616)
                     /gene="dapA"
                     /locus_tag="OO315_000105"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5889173.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            108762..109334
                     /locus_tag="OO315_000106"
     CDS             108762..109334
                     /locus_tag="OO315_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5889174.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            109334..109804
                     /gene="bcp"
                     /locus_tag="OO315_000107"
     CDS             109334..109804
                     /gene="bcp"
                     /locus_tag="OO315_000107"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5889175.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            110057..110674
                     /gene="hyfA"
                     /locus_tag="OO315_000108"
     CDS             110057..110674
                     /gene="hyfA"
                     /locus_tag="OO315_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5889176.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            110674..112691
                     /gene="hyfB"
                     /locus_tag="OO315_000109"
                     /pseudo
     CDS             110674..112691
                     /gene="hyfB"
                     /locus_tag="OO315_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            112702..113649
                     /gene="hyfC"
                     /locus_tag="OO315_000110"
     CDS             112702..113649
                     /gene="hyfC"
                     /locus_tag="OO315_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5889177.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQNYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            113666..115105
                     /gene="hyfD"
                     /locus_tag="OO315_000111"
     CDS             113666..115105
                     /gene="hyfD"
                     /locus_tag="OO315_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5889178.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVDFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            115117..115767
                     /gene="hyfE"
                     /locus_tag="OO315_000112"
     CDS             115117..115767
                     /gene="hyfE"
                     /locus_tag="OO315_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5889179.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            115772..117364
                     /gene="hyfF"
                     /locus_tag="OO315_000113"
     CDS             115772..117364
                     /gene="hyfF"
                     /locus_tag="OO315_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5889180.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMA
                     KPPQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQ
                     RSTWHDFLPSGTASVSEKHSER"
     gene            117354..119068
                     /gene="hyfG"
                     /locus_tag="OO315_000114"
                     /pseudo
     CDS             117354..119068
                     /gene="hyfG"
                     /locus_tag="OO315_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            119078..119623
                     /gene="hyfH"
                     /locus_tag="OO315_000115"
     CDS             119078..119623
                     /gene="hyfH"
                     /locus_tag="OO315_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5889181.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            119620..120378
                     /gene="hyfI"
                     /locus_tag="OO315_000116"
     CDS             119620..120378
                     /gene="hyfI"
                     /locus_tag="OO315_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5889182.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            120371..120784
                     /gene="hyfJ"
                     /locus_tag="OO315_000117"
     CDS             120371..120784
                     /gene="hyfJ"
                     /locus_tag="OO315_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5889183.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            120814..122643
                     /gene="hyfR"
                     /locus_tag="OO315_000118"
     CDS             120814..122643
                     /gene="hyfR"
                     /locus_tag="OO315_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5889184.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            122644..122826
                     /locus_tag="OO315_000119"
     CDS             122644..122826
                     /locus_tag="OO315_000119"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR5889185.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            122848..123720
                     /locus_tag="OO315_000120"
                     /pseudo
     CDS             122848..123720
                     /locus_tag="OO315_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(123758..124819)
                     /locus_tag="OO315_000121"
     CDS             complement(123758..124819)
                     /locus_tag="OO315_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5889186.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            125032..126495
                     /gene="bepA"
                     /locus_tag="OO315_000122"
     CDS             125032..126495
                     /gene="bepA"
                     /locus_tag="OO315_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5889187.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            126516..126875
                     /gene="arsC"
                     /locus_tag="OO315_000123"
     CDS             126516..126875
                     /gene="arsC"
                     /locus_tag="OO315_000123"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5889188.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(127008..127754)
                     /gene="hda"
                     /locus_tag="OO315_000124"
     CDS             complement(127008..127754)
                     /gene="hda"
                     /locus_tag="OO315_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5889189.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(127804..129093)
                     /gene="uraA"
                     /locus_tag="OO315_000125"
     CDS             complement(127804..129093)
                     /gene="uraA"
                     /locus_tag="OO315_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5889190.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(129179..129805)
                     /gene="upp"
                     /locus_tag="OO315_000126"
     CDS             complement(129179..129805)
                     /gene="upp"
                     /locus_tag="OO315_000126"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5889191.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            130129..131166
                     /gene="purM"
                     /locus_tag="OO315_000127"
     CDS             130129..131166
                     /gene="purM"
                     /locus_tag="OO315_000127"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5889192.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            131166..131804
                     /gene="purN"
                     /locus_tag="OO315_000128"
     CDS             131166..131804
                     /gene="purN"
                     /locus_tag="OO315_000128"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5889193.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            131976..134042
                     /gene="ppk1"
                     /locus_tag="OO315_000129"
     CDS             131976..134042
                     /gene="ppk1"
                     /locus_tag="OO315_000129"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5889194.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            134047..135588
                     /gene="ppx"
                     /locus_tag="OO315_000130"
     CDS             134047..135588
                     /gene="ppx"
                     /locus_tag="OO315_000130"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5889195.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(135627..>136733)
                     /gene="pdeF"
                     /locus_tag="OO315_000131"
     CDS             complement(135627..>136733)
                     /gene="pdeF"
                     /locus_tag="OO315_000131"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5889196.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT        34133 a        35177 c        33603 g        33820 t
ORIGIN      
        1 gcaaaatcgt catggattta ctaaaacatg catattcgat cacaaaacgc catagttaac
       61 gttaacattt gcgatattca tcgcatttat gaaagtaagg gactatattt ttataaaagt
      121 taacgttaat aattcaccaa atttgcttaa ccaggatgat taaaatgacg caatctcgat
      181 tgcatgcggc gcaaaacgca ctagcaaaac ttcatgagca ccggggtaac actttctatc
      241 cccattttca cctcgcgcct cctgccggat ggatgaacga tccaaacggc ctgatctggt
      301 ttaacgatcg ttatcacgcg ttttatcaac atcacccgat gagcgaacac tggggaccaa
      361 tgcactgggg acatgccacc agtgacgata tgatccactg gcagcatgag cctattgcgc
      421 tagcgctagg agacgataat gacaaagacg ggtgtttttc aggtagtgct gtcgatgaca
      481 atggtgtcct ctcacttatc tacaccggac acgtctggct cgatggtgca ggtaatgacg
      541 atgcaattcg cgaagtacaa tgtctggcta ccagtcggga tggtattcat ttcgagaaac
      601 agggtgtgat cctcactcca ccagaaggaa tcatgcactt ccgcgatcct aaagtgtggc
      661 gtgaagccga cacatggtgg atggtagtcg gggcgaaaga tccaggcaac acggggcaga
      721 tcctgcttta tcgcggcagt tcattgcgtg aatgggcctt cgatcgcgta ctggcccacg
      781 ctgatgcggg tgaaagctat atgtgggaat gtccggactt tttcagcctt ggcgatcagc
      841 attatctgat gttttccccg cagggaatga atgccgaggg atacagttac cgaaatcgct
      901 ttcaaagtgg cgtaataccc ggaatgtggt cgccaggacg actttttgca caatccgggc
      961 attttactga acttgataac gggcatgact tttatgcacc acaaagcttt ttagcgaagg
     1021 atggtcggcg tattgttatc ggctggatgg atatgtggga atcgccaatg ccctcaaaac
     1081 gtgaaggatg ggcaggctgc atgacgctgg cgcgcgagct atcagagagc aatggcaaac
     1141 ttctacaacg cccggttcac gaagctgagt cgttacgcca gcagcatcaa tctgtctctc
     1201 cccgcagaat cagcaataaa tatgttttgc aggaaaacgc gcaagcagtt gagattcagt
     1261 tgcagtgggc gctgaagaac agtgatgccg aacattacgg attacagctc ggcactggaa
     1321 tgcggctgta tattgataac caatctgagc gacttatttt gtggcggtat tacccacacg
     1381 agaatttaga cggctaccgt agtattcccc tcccgcagcg tgacacgctc gccctaagga
     1441 tatttatcga tacatcatcc gtggaagtat ttattaacga cggggaagcg gtgatgagta
     1501 gtcgaatcta tccgcagcca gaagaacggg aactgtcgct ttatgcctcc cacggagtgg
     1561 ctgtgctgca acatggagca ctctggctac tgggttaaca taatatcagg tggaacaacg
     1621 gatcaacagc gggcaaggga tccgcgtcac tcttccccct tcacgacctt caataatatg
     1681 caatgcagct tcccgcccga taatgtcatg tggaagctga attgtggtca gcggcggtaa
     1741 aaacagatgc ccgacgccaa ccagattatc aaagcccatt acagcgacat cctgcgggat
     1801 acgtaccccc ttcgccaaaa gaacctgata agccacaaag gctgcgcgat cgttaccaca
     1861 tatcagaaca tcaaaatctg gtttgcccga tttgaagtga gcattgagta aacttgcgag
     1921 atcggtgtag tgatcatcac ctgttgccat gtgaaattgt ttcacctcag ccagatctcg
     1981 tccagcatca cgccaagcct gctcaaatcc ctgccgacga taccctgttg ccaacgcact
     2041 ttccggtagc cagaagcata atggttgacg atagcccgcc gcgagcaaat gctgtgttga
     2101 ttcatattgt gcagtgtaat catcagggat ataactgggt aacgctgggt catccgccac
     2161 acagttcgcc aatacaatat tttcaccata cagagactca ggcagcgtga tatgtcgcag
     2221 ccccattgta gtatagataa tgccatccgg acggtgggca agcagctgac gtgccgcgcg
     2281 ggcagcgtca tcttcagaaa aaatattgat taaaaaacta ttccagccga actcgctggc
     2341 ggtttgctca atggcaagca gaatatcaac agagaaagga gtggtagccg tgtcctgcgc
     2401 cagcacggcg agagtcgacg gcttacgtcc ttgagcgcgc atcttacggg cggaaagatc
     2461 aggaacataa ttcagggtct ggattgcctg caatacgcgg tcacgcgttg caggacgcac
     2521 agattctgca ttatgcatca cccgggagac tgtcatcatc gacactcccg ccaggcgtgc
     2581 gacatccttt aatgaagcca tacccaagcc gtttgccgta aaacgggcac tgtagcagaa
     2641 acagacgtca ctggcgagat ccaacgccct atcacctgac acagcaatac aataaaaaat
     2701 aacaataatt cccggacaat tgtccccaat tccgcctctg ttctcgccaa cgagtaacca
     2761 tataggcaag taagtaaaac atgccaactg gcatcgccaa cctggagaag aaaatataaa
     2821 tatggatcat tgtgctgcca ctgtatcccg acatcagccc ggaaattatt gcgattgcta
     2881 tcggttcagg tgcaattggc tgcacgatcg ttacggactc gcttttctgg ctggtgaagc
     2941 aatattgcgg cgctacgctc aatgaaacat ttaaatacta tacgacagcg acatttatcg
     3001 cttcagtcgt cgctctggcg ggcacattcc tgctgtcatt tatcatctaa gcgcaaagag
     3061 acgtactatg gaaaacgcta aaatgaactc gctcatcgcc cagtatccgt tggtaaagga
     3121 tctggttgct cttaaagaaa ccacctggtt taatcctagc acgacctcat tggctgaagg
     3181 tttaccttat gttggcctga ccgaacagga tgttcaggac gcccatgcgc gcttatcccg
     3241 ttttgcaccc tatctggcaa aagcatttgc tgaaacagct gccactgggg ggattattga
     3301 atcagaactg gttgccattc cggctatgca aaaacggctg gaaaaggaat atcaacaacc
     3361 gatcagcggg caactcctac tgaaaaaaga tagccatttg cccatttccg gctctataaa
     3421 agcacgcggc gggatttatg aagtcctggc acatgcagaa aaactggctc tggaagcggg
     3481 gttgctgacg cttgatgatg actacagcaa actgctttct ccggagttta aacagttctt
     3541 tagccaatac agcattgctg tgggctcaac cggaaatctg gggttatcaa tcggcattat
     3601 gagcgcccgc attggcttta aggtgacagt tcatatgtct gctgatgccc gggcatggaa
     3661 aaaagcgaaa ctgcgcagcc atggcgttac ggtcgtggaa tatgagcaag attatggtgt
     3721 tgccgtcgag gaaggacgta aagcagcgca gtctgacccg aactgtttct ttattgatga
     3781 cgaaaattcc cgcacgttgt tccttgggta ttccgttgct ggccagcgtc ttaaagcgca
     3841 atttgcccag caaggccgta tcgtcgatgc tgataaccct ctgtttgtct atctgccgtg
     3901 tggtgttggc ggtggtcctg gtggcgtcgc attcgggctt aaactggcgt ttggcgatca
     3961 tgttcactgc ttttttgccg aaccaacgca ctccccttgt atgttgttag gcgtccatac
     4021 aggattacac gatcagattt ctgttcagga tattggtatc gacaacctta ccgcagcgga
     4081 tggccttgca gttggtcgcg catcatgctt tgtcgggcgg gcaatggagc gtctgctgga
     4141 tggcttctat acccttagcg atcaaaccat gtatgacatg cttggctggc tggcgcagga
     4201 agaaggtatt cgtcttgaac cttcagcact ggcgggtatg gccggacctc agcgcgtgtg
     4261 tgcatcagta agttaccaac agatgcacgg tttcagcgca gaacaattgc gtaatgccac
     4321 tcatctggtg tgggcgacgg gaggtggaat ggtgccggaa gaagagatga atcaatatct
     4381 ggcaaaaggc cgttaataac gtttcaacgc aacatcgcaa tcctttccct gggtgagcga
     4441 tgctgccgat ggcgcagact taagatcccc ggtcttaccc gctataaccc cctcctttta
     4501 tcatcaccca acgcctttcg ctgtaaacgg cggtttcgca aaccaaacca aaaccgtaag
     4561 taagataaaa gcaatagccg ccatacggaa aatctcattt gcagaaatag aaagtgactg
     4621 ctgggttatt tcattattaa tttcattaag aactcctgaa agcgaaccat agtatttatc
     4681 cataatttgt gacgatgaat taaacacggg gttaaattga tcgatggttg ctgtcaactg
     4741 actatggtgt aacgattcgc gtcgtcccca cagcgtcatt gtcaacgacg taccaactga
     4801 tcctgacaag gtacgaaaaa aattactcat actcgaggca ttggcaaatt tattatctgg
     4861 caagcctgaa aacgaaatcg ttgttaaggg taaaaagaaa caggcaacgg cgaatccctg
     4921 gaaaaactgt ggcaaaatga tgcctgtaaa atcaatcgtt ggcataaatg tcacagaacg
     4981 ccagtaatag caaaccgcat acatcaaaaa actgaatgtc actaacaccc gcatgtctat
     5041 tttattgcca taacgtccta tcaaaggtga aatcaatagt ggcatgatgc cgatgggcgc
     5101 ataagcaagc ccggcccata tcgcattata ccccatcgtt ttctggagta actgcggcat
     5161 aaggacgatc gctccagagt aaaataaata cgcgcaggtg atactcacaa taccaatggt
     5221 gaaattacgg gacttaaaca aactgagatc aagaatcggg ttctctgagg tcgattccca
     5281 aatgactaaa gagatcagag aaataactga tactactgtt aatattatta ttgtactcga
     5341 gttgaaccaa tccagatcgc gccctttatc aagcataatt tgcaagccac caacaccgag
     5401 cactaacagg gtcagtcttg gtagattcat ttttaccggt gaagtttcag tttctcttcc
     5461 tttaagtaag gttaagcata atgtcaggac gataatcccc atagggacat tgattaaaaa
     5521 tatccaaccc cagctaaagt tatcgcaaat atagccgccc aatatcggcc cacatatcgg
     5581 agcgataatc acggtcattg accataatgc cagagcaaat gttctttttt ctggtggata
     5641 attccttaat aataaactct gtgacagtgg aattaacggc cccgccatta atccctgaac
     5701 gactctaaaa aatatcagca catcaagatt ggtcgataaa ctacacatta aggaagacag
     5761 cgaaaaaaaa gtgactgaaa gtaaaaataa ccttaattcg cctattcttt gtgccagcct
     5821 gccggtaaca gggatcgcaa tggcatttgc tacgccaaac gaggtgataa cccaggtgcc
     5881 ttcgtctgtt gatgctccca gaaagccaga tattgtcggt attgcgacgt tagaaatagt
     5941 ggaatccaac atttgcataa acgtcgctaa tgacaatgca atagtaacgc accataacgt
     6001 cccaccggtt aatggtgccg gagttgattt agtgattgcc attaaagttg tccattatgc
     6061 gaaataatgt tgctaatttc tttttctatc ggactggtat cgataactaa agccttactg
     6121 gtataagcag gcatggaggt cacggttgaa gccagatcag gcatctcagc aatgtcttcg
     6181 tccttcgtat caatagttgc agtcatcgat aaaccaatgc gcaaggggtg ttccatgagt
     6241 tcttttggat caagagaaac ttcaaccggt acacgctgaa cgattttgat ccagttccct
     6301 gtcgcatttt gtgcaggtaa taaggagaac gcattgccgg ttcccatatt gatccctgtc
     6361 acccgaccat gaaacacaac attttcacca taaagatcgc tgataatatt gactgattga
     6421 ccaatccgta catccgtgag ttgtgtttct ttaaagttgg cattaaccca catttgacgt
     6481 gccggtacga cagccattaa cgattgtccg gaactcactg tttcgccgac ctgaacactt
     6541 cgctgggcaa tatagccagt aaccggactc cttatatccg tacgtttaag caccaaccag
     6601 gcttctttgg ttgcatccgc cgcttcaact acttgtggct gacggtttaa tggtgtgttc
     6661 attactaaag ctttattcgc tttataagcc tggatagcgg cattcagtgc cgctttgcta
     6721 cttattaacg tatctttggt atgctccagc gtttcttttg aaataacccc ctgcttcgct
     6781 aaaggcacac gacggttata atcttctaaa gattgttgat actgaatacg tgctgaagcg
     6841 acttcggcac tgtattgttt atcctgtaag tacagtttat tcgtttgccg aacaatattt
     6901 gccagattat ttttagcttt attgagtgcg atagtggcat cagttttatc cagtgaaact
     6961 aaaatgtcac cctgtcgaac gtagttcgta tctttatgat taactacagt gacactacct
     7021 gagacttgtg cagaaattgg atctgcattc cccgtgacat aggcgtcatc tgtactaatc
     7081 atgtcttcta attccattga ccaataggca taggcacctg aaaacgcaat aaataaaact
     7141 atcaccaata aagaaaagta tttttttctg ttagactgtt ttttatttga attaatctgt
     7201 tccactatta tctctcattt cgcatagatg aaatttatga attgaatgaa aagagtattc
     7261 attcttcata tcaaacacag tgcatgtgtt gcacattatc ccaggaacgt tggaggccta
     7321 atttaaatga ggtgcaaaaa aaacaacaga ggactaaacc gtggcttttg caatacaatt
     7381 cttacgcctg taggattagt gagaagactt atagtgctaa cttgaaacta taaatcatcg
     7441 gtataatccc tgattttatt gttgacattt catttatgcc gactatttat atggtatact
     7501 tgtcgaatta tcttaaagga aactcagatt ttcttatttt tattgagaaa atgagatgac
     7561 gccttatgtc tgtattacta cagggagaag ggagatgctt cattgcaaag ggaataatct
     7621 atgaacgcaa taattattga tgaccatcct cttgctatcg cagcaattcg taatttattg
     7681 atcaaaaacg atattgaaat cttagcggag ttaactgaag gcggaagcgc cgttcagcgg
     7741 gtggaaacac ttaagcctga tatcgtcatc attgatgtcg atatccccgg agttaacggt
     7801 atccaggtgt tagaaacgct gaggaagcgc caatatagcg gaattattat tatcgtctcc
     7861 gctaaaaatg accattttta cgggaaacat tgtgctgatg ctggcgctaa tggtttcgtg
     7921 agtaaaaaag aaggcatgaa caatatcatt gcggctattg aagctgcaaa aaatggctac
     7981 tgctatttcc ccttctctct caaccggttt gttggaagtt taacgtccga ccagcaaaaa
     8041 ctcgactcct tatcgaaaca agaaattagt gtcatgcggt atattcttga tggcaaggat
     8101 aataatgaca ttgcggaaaa aatgttcatc agcaacaaaa ctgtcagcac gtataaaagt
     8161 cgcctgatgg aaaaattaga atgtaaatca ctgatggatc tttacacatt cgcgcaacgt
     8221 aacaaaatcg gctaatcaca tgaagttttt accctatttt tttcttctct gttgtggtct
     8281 ttggtcgacc ataagtttcg cagacgaaga ttacatcgaa tatcgtggca tcagtagtaa
     8341 caaccgtgtc acacttgatc cagtacgtct gagcaacaag gaattacgtt ggttagcgag
     8401 caaaaaaaat cttgtgattg cagtacacaa gtcccaaacg gctaccttgt tgcataccga
     8461 ttcgcagcaa caggttcgtg gtattaatgc tgattattta aatcttttaa aaagagcgtt
     8521 aaatatcaaa ttaacactcc gggaatacgc agatcatcaa aaagcaatgg acgcgcttgc
     8581 agaaggtgaa gtcgatatag tgttatcaca tttagttact tcgccgcctc ttaataatga
     8641 cattgctgca accaaaccat tgataattac ctttccggcg ctggtaacca cccttcacga
     8701 ctcaatgcga ccgcttacct caccaaaacc agtaaatatt gctcgggtag caaattaccc
     8761 cccagacgag gtaattcatc aatcatttcc aaaagcaaca attatctctt ttacaaattt
     8821 atatcaggca ttagcatccg tctcagctgg gcacaatgat tactttattg gtagtaacat
     8881 cattaccagc agtatgattt cccgctattt cactcactcc ttaaatgtag tgaaatatta
     8941 taactcgccg cgtcaatata attttttctt gaccagaaaa gaatctgtca ttcttaatga
     9001 agtactcaat agatttgttg atgctttaac aaatgaagtt cgctatgaag tatcacaaaa
     9061 ttggcttgat acaggaaacc tggcctttct gaacaaacca ttagaactca ctgaacatga
     9121 aaaacagtgg attaagcagc atcccaattt aaaggtgctg gaaaatcctt actcgccccc
     9181 ctattctatg acggatgaaa atggctcggt tcggggcgtt atgggggaca ttcttaatat
     9241 tattaccttg caaacaggtt taaatttttc tccgatcacc gtttcacaca atatccatgc
     9301 tggaacacaa cttagccccg gaggatggga tataatacct ggcgctattt atagtgaaga
     9361 tcgagaaaat aatgttttat ttgctgaagc cttcataaca acgccttacg tttttgtcat
     9421 gcaaaaagcg cctgacagtg aacaaacatt aaaaaaagga atgaaagttg ccattccata
     9481 ttattatgag ctgcattcgc aattaaaaga gatgtatccg gaggttgaat ggatacaggt
     9541 cgataatgcc agcgctgcat ttcacaagat taaggaaggt gaacttgatg ctctggtcgc
     9601 gacacagcta aattcgcgtt acatgatcga tcattactat cctaatgaac tttatcattt
     9661 tcttattcct ggcgttccga atgcatcgct ttcgttcgct tttcctcgcg gagaaccgga
     9721 acttaaggat attattaata aagcactgaa tgcaattccc ccaagcgaag ttctgcgcct
     9781 gacggaaaaa tggattaaaa tgcccaatgt gaccattgac acatgggacc tatatagcga
     9841 gcaattttat attgttacga cattatccgt tttattagtt ggcagtagcc ttttatgggg
     9901 attctacctg ttacgctcag ttcgtcgtcg taaagtcatt cagggtgatt tagaaaacca
     9961 aatatcattc cgaaaagcac tctcggattc cttaccgaat ccaacttatg ttgtaaactg
    10021 gcaaggtaat gtcattagtc ataatagtgc ttttgaacat tatttcactg cggattacta
    10081 caaaaatgca atgttaccat tagaaaacag tgattcaccc tttaaagatg ttttttctaa
    10141 tgcgcatgaa gtcacagcag aaacgaaaga aaatcgaaca atatacacac aggtatttga
    10201 aattgataat ggcatcgaga aaagatgcat taatcactgg catacattat gcaatcttcc
    10261 tgcaagtgac aatgcagtat atatttgtgg ttggcaagat attactgaaa cgcgtgatct
    10321 aattaatgca ctcgaggtag aaaaaaataa agcgataaag gctaccgtag caaaaagtca
    10381 gtttctggca acgatgagtc acgaaataag aacaccaata agctctatta tgggcttcct
    10441 ggaacttctg tcgggttctg gtcttagcaa ggagcaacgg gtggaggcga tttcacttgc
    10501 ctacgccacc ggacaatcac tcctcggctt aattggtgaa atccttgatg tcgacaaaat
    10561 tgaatcgggt aactatcaac ttcaaccaca atgggtcgat atccctactt tagtccagaa
    10621 cacttgtcac tctttcggtg cgattgctgc aagcaaatcg atcgcattaa gttgcagcag
    10681 tacatttcct gatcattacc tggttaagat cgaccctcag gcgtttaagc aggtcttatc
    10741 aaatttactg agtaatgctc tcaaatttac caccgagggg gcagtaaaaa ttacgacctc
    10801 cctgggtcac attgatgaca accacgctgt tatcaaaatg acgattatgg attctggaag
    10861 tggattatcg caggaagaac aacaacaact gtttaagcgc tacagccaaa caagtgcagg
    10921 tcgtcagcaa acaggttctg gtttaggctt aatgatctgc aaagaattaa ttaaaaatat
    10981 gcagggcgat ttatcattag aaagtcatcc aggcatagga acaacattta cgatcacaat
    11041 cccggtagaa attagccagc aagtggcgac tgtcgaggca aaagcagaac aacccatcac
    11101 actacctgaa aagttgagca tattaatcgc ggatgatcat ccgaccaaca ggctattact
    11161 caaacgccag ctaaatctat taggatatga tgttgatgaa gccactgatg gtgtgcaagc
    11221 gctacacaaa gtcagtatgc aacattatga tctgcttatt actgacgtta atatgccgaa
    11281 tatggatggt tttgagttga ctcgcaaact ccgtgagcaa aattcttcct tacccatctg
    11341 ggggcttaca gccaacgcac aggctaacga acgtgaaaaa gggttaagtt gcggcatgaa
    11401 cttatgtttg ttcaaaccgt tgaccctgga tgtactgaaa acacatttaa gtcagttaca
    11461 ccaggttgcg catattgcac ctcagtatcg ccaccttgat atcgaagccc tgaaaaataa
    11521 tacggcgaat gatctacaac tgatgcagga gattctcatg actttccagc atgaaacgca
    11581 taaagatcta cccgctgcgt ttcatgcact agaagctggc gataacagaa ctttccatca
    11641 gtgtattcat cgcatccacg gtgcggctaa catcctgaat ttgcaaaagt tgattaatat
    11701 tagccatcag ttagaaataa cacctgcttc agatgacagt aagcctgaaa ttcttcagct
    11761 gctgaactct gttaaagaac acattgcaga gctggaccag gagattgctg ttttctgtca
    11821 gaaaaatgac taaattgcgg ctcccacaat gttctaatgt gggagctatt taccagcaca
    11881 tcttacattt atgatgagaa ctcctggcga atttgttccc catgctggtc gagcgttgcc
    11941 gctcccggca taacatgcgg gtccgcgcag ccgctgattt ttatcggatt acccggcatc
    12001 attattcctc cggcttcaat caacatattt ctcgcctgag tttgtggcaa attaatggcc
    12061 tcagccacac ttaataacgg cgcgacgggc acaccaactt catgtatttt ggctaaccaa
    12121 acttcagctg cctgcgtttt taacgttcgc tcaatatatt gtttaagaat agcctggttt
    12181 tgtacgcgta aaatattgct gctaaatcgg ggatcattaa ccagttccgt aagctccagt
    12241 gcctggcata acgcagaaaa aagcttgtca ttaccacaac aaatcgtaat cggcttatcc
    12301 tgagtatcga aaacatcaaa aggtgccatg tagggatggc gatttcccag gcgttgtggt
    12361 gacttccctg tcgcaatata tgccatcaga ccatgctcca gaaaactcag cgtggcatca
    12421 aacatcgcta tatcgacatg cgcccctctc tggctctttt cgcggccata aagtgcactc
    12481 actattccgc tgaataaata gacaccgccg catagatccg caagagaggt accaacacgc
    12541 actggcggag catcagggta ccctgtttcc atcattatcc cactcattgc ctgaatgatg
    12601 gtatcgtagg caggagcatc ttttagcgga ccggtatgtc cgaaacctga cgatgaagca
    12661 tatatgaggc gcgggttgat ttcttgtaac ctttcccatg aaaaccccag tttttccatt
    12721 gtacctaggc gaaaattctc agctaataca tcagcttgtt tgagcatatt tataaatata
    12781 cttttatcgt gatcattctt taaatcaaga accacactct ctttgccatg attaataaaa
    12841 ctgtaataga gtgactgtcc atccacatag ggaccaaatg tgcgggtatc atcaccatga
    12901 cctggtggct caactttaat tacccttgcg cccatattat aaagaagttg agttccgaaa
    12961 ggtccattaa ggacatgtgt catatcgata actaataagc cttcaaacgg ccctttgctt
    13021 tcattatttg tcatttgctt atgccttgaa aaaagccgaa ggtgaaaaga cacttcggca
    13081 tttatgtaaa atcaataaca tgattaatta tgaaaccagt cgactgacat aaatccacaa
    13141 gggagccgtg acgacaaagc ccagtacgct cacagccaat gacgctgtac cagtacgggt
    13201 gtagacatta aaccggctgg caataatgat tccggagaat gccggcggta atgcgcctgc
    13261 cagtaccatc atctgcaaat gttcgctgtt caaatgacat gccataccaa cgagaagcag
    13321 cgccagtggc atcagaatca acttcaggaa ggtgttataa gcaatttcgg cactgaactc
    13381 gaatttatgt gcagccagag tcaaccccgc agcgaatacc gctacccctg agttagcctt
    13441 cgcaatcaga ttaaaggttg ggtcccatgc tgccggaatt tttaccccaa ccagcaccag
    13501 gatcgttgcc agaacaggtg cccatactac tggctccttt gccgcagaaa ttaatgcgct
    13561 cagattacta ttcttcttac catccgctcc tgaggaagga ttcagcaaat acagaccaat
    13621 aggaatagta attgcgttaa caataataga aataattgct accactaaac ctgttgatac
    13681 ggaatcacca taaataggat cgagaactgc aaaccccagg aatccaatgg taggtgaacc
    13741 cgcaattaat gcacatacag ctgcttctgc atgggtacgt ttaaaaaatt tgtagcaacc
    13801 aaaccaggag aaaaagaaac atccgacaat aaccacaagt gatacaaggg tcagacgagt
    13861 gtccgcaaaa atcatttccc tgtttgcccg ggtaatagaa acaaatagag ccgcaggaag
    13921 agcgtagttt aataccagtt tattaaatgc gcgggcttga tcttctgaaa aagtttctcg
    13981 tctgccgcta aaataaccca acagcatgat tacgataata ggcaataaat cgccaataaa
    14041 aaatgttagc ataatagtat ttccgataag ggtcccctca ccatcgcata atgagttggc
    14101 aagaggagac gatgtcagta tttaattacc agcgacttgt tttgggttaa gtttggtaat
    14161 atggccactt tcagttcctg ctgcagggtc gataaccaca ttaataatgg tcggtttgcg
    14221 cgactgaata ccggtggtta aagcatgacg aagttcatct gtcgtggtga cgttatagcc
    14281 aacgccacga aacgcatcca ttaatttgtc ataccttgca tggtgcaaca gatccgttgg
    14341 tgatggtgca ccagcgccac tgagatcaac accgtctcct ctgtagatgc cgccattatt
    14401 aaaaataacg atcgtcaccg gcaggttata tcgacaaatc gtttcaattt ccatcccact
    14461 gaaaccaaaa gcactatcac cttcaatggc gacaaccgga gaaccagaag tcacgctagc
    14521 accgatggca tagcccatac cgatgcccat gacaccccag gtaccacaat ccagacgacg
    14581 acgtggttta tacatatcaa taatatttcg tgcattatcc agggtatttg caccttcatt
    14641 aactaaataa atatcctggt tctcgcgcaa tacgtcgcgc acagcactta atgcattaaa
    14701 gtaatttaat ggttgagtat ctgtacttaa tttttcatgc attttttgtg cattttgctg
    14761 cttgtggata tttaaaatat cgcgccatac cagtggagtc gtaaatgtgt tttgtttcag
    14821 ttctgccagc ataccttgca tactggatgc aatatcacca acgactggca cagcaatggg
    14881 gcggttgctg tcaatttcct gcggttcaat atccagttga ataaactgtg tatctgccgc
    14941 ccatcctttt ttaccgtgtg ccaataacca attcagtcgt gcaccaacaa gcatgacaac
    15001 gtcagcattt gccagggcaa acgaacgcgc agctgccgca gaaagtggat gcgtatcttc
    15061 aaggatccct ttcgccatag acattggcag gaatggaatc tgagcacttt caataaattc
    15121 acgaagctgt tcatcagctt gtgaatacgc cgcgcctttg ccaaggataa ttaatggccg
    15181 ttcagctttt gctaaaagcg aaattgcgct agtgactgac ttcgggcatg gcaataatgc
    15241 tggcgacgga ttttcaactt taacaatcgt ggttaacgct tcgtcttttt ccatcgtcgc
    15301 ggccaggaca tttgctggca aatcaagata aactccgcca gggcgacccg atacagagac
    15361 ccggatagcg cgtgccaatg caatgccaag atcctgcggc tgattaacgc gaaatgctgc
    15421 tttggcatac ggttttgccg cattcatttg gtccagctct tcataatcac cttgctgtag
    15481 gtcgacgatc gcgcggtcgc tggagccgct aatcatgata atcggaaaac catttaccgt
    15541 tgcgttggcc aatgcggtca aaccattgag gaatcctggc gcagaaactg tcaggcagat
    15601 ccccggtttt tgggtaagaa aaccgcttgc cgcagcggca tagcctgccg actgctcatg
    15661 acgaaaacca atataacgaa tgccttccgc ctgggcatgg cgtgccatat ccgtcacagg
    15721 aatacctaca acaccataaa tagtgtcaat attattctgt tttaatgctt caacgatgat
    15781 atgcatacca tctgtcattt gaagttgatc tgacatgatt aataacctcg aaatttttat
    15841 aaattgggag ccagaagccc ccgttaatat cagatggcgt ggttttgctt cattgcagca
    15901 atttcatcgt cgctataacc cagctcctgc aatacagcag cggtatgttc acctaatagc
    15961 ggcgcagctt taatatccgg cgtaaaggca gagaatttca ttggacaacc aacggtcaga
    16021 tattttccac gcaacggttg ttccacttca acaacactgc cactttggcg caaagaggga
    16081 tcaagtgaaa tttctttcat acttaaaacc ggtgcacaag gaatatcaaa ctgagtcaaa
    16141 taggccaccg cttcatgttt atcaatagtg acagtgtatt tttcgatttc agcaaaaata
    16201 tcgaaaatat gtggctgtcg ggcatgggct gtactgtatg ccggatcggt aatccattct
    16261 ggtttgccga tggctttaca ggtgttttcc cagttttgct cctgaatagt gaaataaata
    16321 taggcgttag gatcggtttc ccagccttta catttcagga tccagccagg ctgaccgcca
    16381 ccacctgcat taccaccgcg gggaactgca tcaccaaatg taccattcgg atactgcggg
    16441 tattcttcca gataacccaa tttatcgaga cgctgctggt cacgtaattt cacgcggcaa
    16501 aggttcaata cggcatcctg cattgacatg gtgactcgtt gcccacgccc cgttttttcg
    16561 cgatgcagca aagcagcaag taaaccgatc agcaaatgca ttccggtgtt gctgtcaccc
    16621 aacgctgcag cgcttaccag cggcggacca tcccaaaaac ccgtagtgga tgccgcgcca
    16681 cccgctgcct gagcaacgtt ttcataggct tttacattca cataaggcga acactcatca
    16741 aaccctttga tcgaaccaaa aatcagacgt ggattgattt cttgaatatg ctcccaggtg
    16801 aagcccatgt gatcaatggc ccctggatga aagttctcga ctaagatatc agcttcgcgg
    16861 atcagctttt ccattacctc tttgccttcc gctgttttgg tatttaactc aatagaacgt
    16921 ttgttactgt taagcatggt gaagtaaagc gcatcgatat caggaatatc tcgcagttgg
    16981 tgacgcgtta cgtcaccaac gccgggacgt tcaattttaa taacgtcagc gccaaaccag
    17041 gccagcattt gagtacaaga tgggccagat tgcacaccgg tgaaatcgag aactttaatt
    17101 ccttgaagtg gagttgacat agcaatacct attagttaat taaatatgtg ttaatttagc
    17161 gtttatttaa ggcataacgc cctctataaa taatctgcaa aatatcttcg tagcggcact
    17221 cgatattttt aaattcctgt atcaagtact gaaaattatt ttatcgccac ttccaattcc
    17281 tttctgcatg gtaagtagtg aggacgaaaa gaagaatatg ataaatatta attcgtggta
    17341 atcagagaga gtcgcgacat ttgtgatgtt attcttacaa ctgtaagaag cgccggtgat
    17401 ttacaagtaa gagtatgatt acgcttttta aagcgtacca taaagcataa cataataaat
    17461 gatttgtgat gacacgggat tattgcggtt tactttgagg aaaataaaat gcgtgtttat
    17521 agaaccgcga gcgccaaata attttatgac cacaacctgt cgtattgtgg tcataaaaaa
    17581 ttgtcggctg gagggagtta aatcctttac ctcaatatac tgcctgtcag cattagttca
    17641 caaacagtgc ttcgctatcg acgatgattc catcttccgc acctttaagc gccaggtttg
    17701 cctcgtagta ttgcttttta tcaagcagtt tctgcgcatc agcaagtgca ttacgagtct
    17761 gtttaagcgg catcagatat tggttttcca ttacgccaac accggccagg cgaagctctt
    17821 ccattgcgcc ctttttatcc cctttcgcca ttttttcatt ggcaattttt atcgcggctt
    17881 ctttttcggg tgttgctaca taactctcag aaattccgac agatgcatta atgacaatat
    17941 attggtcatc attgagattg gtctttttac ctggcttagc gaactttgcc cattcggtac
    18001 tgtcatctga cagcaaagcg gaagcttcat tggtcagttc ttttgccttt tccggatcgc
    18061 catgaaacag agccaggcgc gctacctgca cgtcacgcat cgcatataaa ccttgttcag
    18121 agatacgctc tgctgctgcc gttttctggg tttgctgagt ttgctgttgt gccgctaccg
    18181 gtgcattatc agcagcccat acagttgaag atgccagaat tgctgtgacc atcgtagcca
    18241 taattaaacg tttcatactt acctcattct ttaacagttt tagaaaacgc cgccatcata
    18301 ttgggtgagc atgaaatgag aaatcagaga gcaacggtca ttttgaaagg gttatcttac
    18361 aattgtagtt attatcgcaa agcggcattt agtcttacag ttgtaggaat atgcctcctg
    18421 tcgcagagaa ctattcttat ataccacaaa gagatgtcat ttattatatt gatgtaaata
    18481 ttgattaatt atatgtaaat acagccataa catcgtatgg ttctcagtga accattttca
    18541 ggataacttt ctatgaaagt aaacttaata cttttcagct tatttttatt ggtctctatt
    18601 atggcatgca atgtttttgc attttccatt tctggtggtg taagtgagag gagctataaa
    18661 gagactgaaa aaacatcagc gatgacgacc acacactcta caaaacttca gccatcacag
    18721 gcgattttgt ttaagatgag agaagatgtg ccaccattaa acctcacaga agaaatcacg
    18781 cctacttatc cgacaaaggc gaattatctt attcatcctg tgcgatagcg tctgtcacat
    18841 ggcaggtgaa caatcatgtc tgatgctgat gtgaacacct gcccaaatga gccatcctga
    18901 cccactatcg cctgccatac cccaccataa agcgaatcat caagccacaa gccggggcaa
    18961 gaaacataaa tatccccgtc accagttcat taccgccagt taaccacatg gtgaagtaat
    19021 aaatgagtgc agcgaggatc accatgccga agaaagaaga gaccgccgat accacgttca
    19081 gtacctgacc gatgtagccg cttacgcaga caatacacag ggcgagaaac atccataaat
    19141 aaatcatagc tcaacctcct gccgcgctaa aaaacctgta acttcagtca ctttaatagt
    19201 aagacaatcc ttaacctctg gcaatttttg ctaagcatca atgaaaacag cacgttaaat
    19261 gcaacgctat tgcgtgcgga aaatttttta ctttgcacga ttaataatca gtagctgaaa
    19321 gcagtcagcc agtgataaag tagagccacc gcattcatgc tcctcgtggt tatgtcctga
    19381 ctagtcttta cactctttac aggaaccatt gtcgtacatg atggcccaac caattgagtg
    19441 taccgctgtc gacatggaat cttctgcgat acaacaattc gtatctacag aaggtaacta
    19501 tgtttccaca atgcaaattt tcccgcgagt ttctacatcc tcgctactgg ctcacatggt
    19561 ttgggcttgg tgtactctgg ctttgggtac agcttcctta tcctgttctc tgctttctcg
    19621 gcacgcgtat tggcgcaatg gcgcgaccat tcctgaaacg tcgtgaatct atcgcccgta
    19681 aaaacctgga actttgtttc ccgcagcatt ctgcggaaga acgcgagaag atgattgccg
    19741 aaaactttcg ttcactcggc atggcgctgg tagaaaccgg catggcatgg ttctggcccg
    19801 acagtcgcgt acgtaaatgg tttgatgttg aagggttgga taaccttaaa cgcgcacaaa
    19861 tgcaaaatcg cggcgtaatg gttgtcggcg tccattttat gtcgctggaa ctgggcggcc
    19921 gcgtgatggg actgtgccaa ccaatgatgg ctacctatcg tccacataat aatcagctga
    19981 tggaatgggt gcagacccgt gggcgcatgc gctctaacaa agcgatgatc ggcagaaata
    20041 atctgcgcgg cattgtcggt gcactgaaga aaggtgaagc ggtatggttt gctcccgatc
    20101 aggattatgg tcgtaaaggc agctccttcg cgccgttctt tgcggtggaa aatgtcgcca
    20161 caaccaatgg cacctatgtt ctctcccgtc tctctggcgc agccatgttg accgtaacga
    20221 tggtaagaaa agcggattac agcggatatc gtttgttcat caccccagag atggaaggct
    20281 acccgacaga tgaaaatcaa gccgctgctt atatgaacaa gattatcgag aaagagatca
    20341 tgcgcgcacc ggagcagtac ctctggatcc accgtcgctt taaaacgcgc ccggtgggag
    20401 aatcgtcgtt gtacatttaa caaactgctc ttactctttc cctcctcaaa tcggccataa
    20461 taacctcatg atttttatag cttttttctt aagttttttc cttcgctgaa tatctaatta
    20521 ttaccgtcag aaattaaact gtcgccgata tgagacactt gtaagttact aaaagtaaat
    20581 aagaaaatgc ctcttgtctc gtttcagttt taggcgtaca ttagcgccgt ctcgagcagt
    20641 ttagctcaga attctggggt gatgcccacg gtaaaataca gggcttttaa ggtcgctttg
    20701 cccggtgtca actcactgta tcaggtgtaa tgaagtcatt caggcgtaac agtaattacg
    20761 cggagagatg taaagtgaaa tatttcttta tgggcatttc ttttatggtc atcgtttggg
    20821 ccggtacttt tgccctgatg atctaaaagc aaactgtgca atgaaaaaca ggagccatca
    20881 ggctcctgtt tgcatttctg acgacggtgt ttattccgcg ttttcgtgaa tatgtttgct
    20941 gctgacgggt agtaaaccgt cggcacggaa catcgcttta atccctctga tggcctgacg
    21001 aatacggtcg tggttttcaa tcagtgcaaa gcgaacatgc gtatcaccat agtcgccaaa
    21061 gccaatcccc ggcgagacac aaaccttcgc ttcgttaagg agcttcttgg caaattccag
    21121 cgatcccatg gccgcatatg gttccgggat tttcgcccag acatacatcg aagccttcgg
    21181 catttcgacc atccagcccg cttcatgcag ccctttaacc aatacatcac gacggcgttt
    21241 gtactgttcg gcaatgtcgc gcacgcactg ttgatcgccc tcgagtgccg caatcgccgc
    21301 cacctgcaac ggcgtaaaag tgccgtaatc gtgatagctt ttgattcgcg ccagggcact
    21361 gaccagcgtt ttgttgccga ccataaagcc gatacgccag cccgccatgt tgtagctttt
    21421 cgacagcgta aagaactcga ccgccacatc gcgtgcaccc ggcacctgca taattgacgg
    21481 cgctttccag ccatcgtaga cgatatcggc ataggccagg tcatggacca ccagcacatc
    21541 gtagcatttc gccagcgcca caaccttttc gaagaactcc agttccacgc attgcgcggt
    21601 tgggttcgac gggaagccga ggatcatcat cttcggtttc ggataacttt cacgaatggc
    21661 gcgctccagt tcgttgaaga aatcgacacc ttccaccagc ggcactgagc gtacctgcgc
    21721 cccggcaatc accgcgccat aaatatggat cgggtaactt ggattcggca ccagcaccgt
    21781 gtcaccatga tccagcgtcg ccagcatcag atgcgccagg ccctctttcg aaccaatagt
    21841 gacgatggct tctgattccg ggtcgatttc aacgtcgtag cgatcctgat accagcggga
    21901 aatggcgcga cgtaaccgcg gaatgccgcg tgaagtggag taaccatgcg tgtccgggcg
    21961 ctgggccaca gtacataatt tttcgacgat atgcggcgga gtcgcaccgt ccgggttacc
    22021 catgctgaaa tcgataatat cttcgccgcg ccgacgcgca gccattttca gttcagcggt
    22081 gatgttaaaa acgtagggcg ggagacgatc aatgcgcgta aagcgacgtt cagggcgagt
    22141 gtcagccata atttcctcag attaacgtta gcgcccggac cgtccgagcg acgctgccac
    22201 aatggtggct gttttgaaaa tagcctgatt aatttctgtc tgtccagcgt tttagaacat
    22261 ttttatcaat ggatttaaat gaggaactcc acttcaaaaa caggaaattt ttctacgcta
    22321 acaagaaaat caccaccaat tttattaaca ttataatttc aataaattta ccagccagaa
    22381 gtgcattatc aagagataca ttgctaatac accatttatc ttttaatccc cttttaaatt
    22441 tatggttttt cctcaaaaac ggcctgctgg tcatcagcct tcaggttacc tatcatagag
    22501 gtttaatcct tattcagagt cacccgtgca cgaaatattc aacatgctgc tggcggtctt
    22561 cgatcgggca gcgttaatgc ttatctgcct gttctttctc atccgtatcc gcctgtttcg
    22621 cgaactgttg cacaagtcgg cgcactcccc aaaagaattg ctcgccgtta ccgccatttt
    22681 ctcgctgttc gccctgttca gcacctggtc cggcgttccc gtagaaggct cgctggtgaa
    22741 cgtacgtatt atcgcggtga tgtccggcgg gattctgttt ggcccgtggg taggcatcat
    22801 taccggcgtg attgcgggta ttcaccggta tttaattgat atcggcggcg tgacggcgat
    22861 cccctgcttt atcaccagca ttctggcggg ttgtatatca ggctggataa acctgaaaat
    22921 ccccaaagca cagcgctggc gctggatatc gccaacaaaa cgctaccgct gtttcgccat
    22981 gtcaatagcg agtcattacg caaggtctgc gaaattatcc gcgatgacat tcacgccgat
    23041 gcggtggcga ttactaatac cgatcatgtg ctggcctatg ttggcgtggg tgaacataac
    23101 tatcagaatg gcgatgactt cattagcccg actacccgtc aggcgatgaa ttacggaaaa
    23161 atcatcatta aaaacaatga tgaagcccac cgcacaccag agattcattc catgctggtg
    23221 atcccattgt gggagaaagg ggtcgtgacc ggaacgctga aaatttacta ctgccacgcg
    23281 catcagatca cctcgtcatt acaggaaatg gcggtcggtc tgtcgcaaat catctccacg
    23341 caactggagg tttcacgcgc cgagcagcta cgtgaaatgg caaataaggc agagattcgc
    23401 gccctgcaaa gcaaaattaa tccccatttt ctgtttaacg ctctgaacgc tatttcatcg
    23461 tcaatccgtc tgaatccgga taccgctcgc cagttgatct ttaatctgtc gcgttatctg
    23521 cgctataaca ttgaattaaa agacgatgag caaatcgata tcaaaaaaga gctgtatcaa
    23581 attaaagact atattgccat tgagcaggcc cgctttggcg acaagctgac ggttatctat
    23641 gatattgatg aagaggtgaa ttgctgcatt cccagcctgc tgatccagcc gttggtggag
    23701 aacgccattg tccacggtat tcagccttgc aaaggtaaag gcgttgtcac catcagcgtt
    23761 gcagagtgcg gaaatcgggt acgcattgcg gtgcgagata ccgggcacgg catcgttcca
    23821 aaggtgattg agcgggtcga agcgaatgaa atgccgggca ataaaattgg cctgctgaat
    23881 gtccatcatc gcgtgaagtt attgtatggc gaggggctgc atatccgccg cctggagccg
    23941 gggacggaaa ttgcgtttta cattcctaac caacgcaccc cagtcgcctc acaggctacg
    24001 ttattgcttt gagccggagt gatattgtga aagtcatcat tgttgaagac gaattcctgg
    24061 cacaacagga actgagctgg ctaattaaag agcacagcca gatggagatt gtcggcacct
    24121 ttgacgacgg tctggacgtg ttgaagtttt tgcagcataa ccgcgtcgac gccatttttc
    24181 tggatatcaa tattccgtcg ctggatggcg tgttgctggc gcaaaacatc agccagttcg
    24241 cccataaacc gtttattgtg ttcatcaccg cgtggaaaga acatgcggta gaagcgtttg
    24301 aactggaggc gtttgactac attctcaaac cgtatcagga gtcacgtatt accgggatgc
    24361 tgcaaaaact ggaagcggcc tggcaacaac agcagaccag cagtacgcct gccgcgacgg
    24421 taacgcgtga gaatgacacc attaatctgg tgaaagatga gcgaataatc gtcacgccaa
    24481 ttaacgatat ctattacgcc gaagcgcacg agaaaatgac ctttgtctat acgcggcgtg
    24541 aatcctacgt aatgccgatg aacattaccg aattttgcag caaactgccg ccgtcgcatt
    24601 ttttccgctg ccatcgctca ttttgtgtca atctgaacaa aatacgcgaa atcgaaccgt
    24661 ggtttaataa cacctacatt ctgcgactga aagatctgga ttttgaagtg ccggtcagcc
    24721 gcagcaaagt gaaagaattt cgccagttaa tgcatcttta atcgggaact ttcatgaaag
    24781 caccaggctt gcccgccgat caacaatttt tcgccgatct gttcagcggc ctagtgctta
    24841 acccgcaact actggggcgc gtctggtttg ccagccagcc tgcctcgtta ccggtgggca
    24901 gtttatgtat tgattttccc cgtctggata tcgtgctgcg cggcgaatac ggcaacctgc
    24961 tggaagcaaa gcagcagcga atggtggaag gagaaatgct gtttattccg gcacgcgcgg
    25021 ctaatttacc gatcaacaac aaaccggtga tgctgttaag cctggtgttc gcaccgacct
    25081 ggcttgggtt atcgttttac gatagccgca ccacgtcgtt gttgcatcct gctcgccaga
    25141 cccagcttcc cagcctgcaa cgcggtgaag gtgaagcgat gcttaccgcc ctcacccatc
    25201 ttagccgttc gccgctggag cagaatatca ttcagccact ggtgttaagt ttgctgcatc
    25261 tttgccgcaa cgtggtgaat atgccgccgg gtaattcaca accgcgcggc gattttctct
    25321 atcacagcat ttgtaactgg gtttaggata attatgccca gccgctcacc cgcgagagcg
    25381 tggcgcagtt ttttaatatc acgcccaatc atctgtcaaa actgtttgct cagcatggaa
    25441 cgatgcgttt tatcgagtat gtgcgttggg tgcgaatggc gaaggcgagg atgattttgc
    25501 agaaatatca tctgtcaatt catgaagtgg cacagcgttg cggttttccg gatagcgact
    25561 atttttgtcg cgttttccgg cgtcagttta gtctgacgcc gggagagtac agcgcccgtt
    25621 ttcagggcta acgtcagaag gttaattctg tttccagcag cgtcaggata ctttgtgcat
    25681 cttgcgcggc aaacaacgac tggcggaagt ttttattcac cagtttacgc gccagctgcg
    25741 agaagacttt cacatgattc atcccttcgt tagcacccag cgtcagcatg atcaccagtt
    25801 cgacttcgcc catttctgac tgccagtcaa ccggtttcgc cagccgggca atgctgatac
    25861 tggaatgacg gatccactga gatttggtgt gcgggatcgc tacgccaaaa ccaacgccgg
    25921 tggtaacaat ctcttcccgc tgccagacat cttcttccag ctcaaacgga tgttcagtgc
    25981 gcccgttaac gccgaggttg ccgcacagga actggatcgc ctgctcttta ttgctaaaat
    26041 cctgatcaac aaagatattc tccagcgcca gcagtgggcg aacgtcttct tccggcgtaa
    26101 aggcggtgag taacgcttca atttcctgcg cactgcggca ttcacatgcc tgacgcgcca
    26161 gttcccgaca cgcctcgcta tccagttgac gaagctggct tttcaccgcc ggaatacgcg
    26221 ggctactcat actcagctca tccaggccca gcccaagcag tagcggcaga taacggcttt
    26281 caccgcccag ttcaccgcaa atgcctaccc atttgccccg ctgatgcgca gtggtaacta
    26341 tttgctgcaa catgcgcagg aacgatggcg taatcgggtt atatagcggc gatacgcgcg
    26401 ggttattacg atcgaccgca tacagatact gggtcatatc gttggagccg atactgaaga
    26461 aatcgacctc atcgcagaag tggtcgatga tgtagcacac cgacggcact tccaccatga
    26521 tcccaagcgt aatcgtctct gcatgacgca ggccatcgcg cttaagctca acgatcgctt
    26581 tttggatctc gcctttcacc cataagatct gatcgaggct gtgaaccatc gggatcatca
    26641 actgggcgtt gccgaaactg gcggcgcgca aaatggcccg cagttgagtg cggaacaggc
    26701 cagcaaattc cgggtaaata cgtaccgcgc gatagccgag gaacgggttc tcttcctggg
    26761 gaatattcag ataaggaatg cttttatcgc cgccgatgtc catcgtgcgg aagataatcg
    26821 gcttgtcgcc cgccgccagt agcacctgct ggtaggcttc aaattgctcc tgctcgtccg
    26881 gcgcgctgtc gcgatccata tagagcattt cggtacggaa cagcccgaca ccttccgcgc
    26941 cgttggcaaa cgcgcctggc gcttccagag cggtgccgat attcgccgca atatcaatac
    27001 gcttgttatc acgggaatag gccagctgcg cggcagcctg cgcctgttgt ttttggcgtt
    27061 tatccgccag cgtctgcgcg acctgataat aaccgctcac cgcgtcattc gggttaatcg
    27121 ccagcacgcc gcactgggcg tcaagcactg caggttgccc ggcataacgg gcaatcgcat
    27181 ccagcggcaa gccactcagt accgggatcg ccgaggcacg ggccagaatc agtgtatgcg
    27241 aggtgcgccc ggttttctcc agaatcatac ccgcaagatt tttcaaatca aggctcaaaa
    27301 actggcttgg ggttaaatct tcagccacca gaatggtcgg tttttccagc accagattgt
    27361 tgcgcggctt cagttccggc caggtgatat gcagcaactg ttcgctgata tcgcgaatgt
    27421 cgctgacccg ctcgcgcaga taatcgctgg cagaagcaga tagtttggcg caaacctgct
    27481 ccatattgct gatgatcgcc gcccccagtc cctgatgctg ttctgtcatc aggcgacgga
    27541 tattgcctgc aaattcatca tcctgaatca gcgacaaatg agcgctgagg atagttttgc
    27601 tttcgccgtc acgctcacgc aattgctgat tcagttgctc ggcaagcgtt gccaggctgt
    27661 gctccagccg ggtggaatct tgcgcactgg cggggattgc ccgataactg tcgaggctgt
    27721 cgctttgtaa cagggtcagc gtacccacgc cgacgccgct tgccagcaca ttgccgtaca
    27781 gtaaatccgg gttaaggcgg cttaatgaac gcggcagcgg atgcgccgtc agttctgcct
    27841 gcgtcggctg aacgctgtcg ctgtcgataa agcgcaccag gatgtactct tccagcacgc
    27901 gccgcgcctg ctcttcatcg ctgccgctaa tgttcaggct gcaactgtca ttaaataagg
    27961 tgccagttcc aatcaacgcc agcgagcttt tagcatcagc ctttgcgttc tggcgatggt
    28021 tgataaaggt gatttcgctt tgccactggc tgcactgttc tttaagttcc cacgccggac
    28081 gagcatgtag accgttaggc aaaggacaga gaaattgaat cgttaacata accgctccgg
    28141 gttaatcagg agatcatctg aaatccgtca gttgaacaac cgtctcacgc gtaagacgtt
    28201 gaataagggc agataaaagt tgctccatct gcaaaatgtc gcggcaatcg gcaatcgatg
    28261 ccgcgcaatg tccgtggcgg gtggcaggcc ccattaccaa tgtgggtacg ccagtaccgg
    28321 ttaagtgcac cgctccaccg tccgtgccgc cgttactgaa catatccgcc tgcaacggaa
    28381 cgccaatttc tgccgccacg gtttcaatcc acgcggtaag ttttggcggc gcaatcagcg
    28441 atttatcgct taacaccagc atcggcccgt taccaatctg acgatggtta gccgcgccat
    28501 aatcaaagtt tttcgcccag caggcggtat caagcacaat ggcgacgtcc ggcgacaccg
    28561 cgcgggtagc agtttgcccg ccgcgtaatc ccacctcttc gctggaactt gccaccagcc
    28621 acacttccgc aggcagttcg gcgtcgtgca gctcacgtag taacgtcacc agcagatagc
    28681 aactgaggcg atcatcaaag gctttgccca tcacccgctg gtgagggagg acctgaaaag
    28741 tggtatcgaa ggtgacgcga tcgcctggac gaattcccgc ctgcatcact tcgtcatagg
    28801 tgcgcgcgcc aatgtccacg cgcatggcgc taacgtcatt cccctgccgg tcgccgtcaa
    28861 gcaggcctgg aattttgcac tcttcacggg tggtgatgcg caccggctgc agctggcggg
    28921 cagccatgcg cacgttgcca accggcagca cgtcaatagc gccttcgcgg gagatgctgc
    28981 gcaccataaa tcccacttcg tccatatggg cgcagatcat caccttcgga cctgtcgatt
    29041 cattgaggcg gatcagcacc gatcccagac catcaaatcg cacttctttt tgcaggcgag
    29101 ccgcttcttc cagcaggatc tgccgcactt cctgttccga ggaggcgatc gcatctgcct
    29161 cgctcaacgc ttttaatagc gataaatcca ttatgcctct cccgtgagca acactgtttt
    29221 cggcatggcg tagagcactt ctgcgccttg cggggtaacc agcacaacat cttcgatgcg
    29281 cacgccccct tgccctagca aataaatccc cggctccacg gtcagcaaca tgcctggctg
    29341 tagcgtcgtg gtgtcccgcg gtgaaaaacg cggatcttca tggacttcaa tgccgatagc
    29401 gtgaccagtg ttatgaccga aatagtcgcc aaatccagcc tcggtaatga cccggcgcgc
    29461 ggcgtcgtca atctgctggc agcgcacgcc ggggcgaatt gcagagattg ccgcgagctg
    29521 tgcctgcagg acaatctgat agacgtcaaa cagcgggtga gattcggcgc tcaccccttc
    29581 gccattcacc agcaaggtgc gcgtcatatc agcagtagcc ctgatacagc gcaccaaaat
    29641 cgagagtgac aaactcgccc gctgcaacaa tcttgtcgct ggctttgccg tgcggcagcg
    29701 ccccgcgcca gccgctggcg acaatggtgt caaaagaggc tttttctgcg ccctgctggc
    29761 gcataaacca ctccagttca gcggctatct cgcgctcgct catccccgcc tgaataaagc
    29821 ggcgaatatg ctccgctccg cgatcggcaa tcccacaggc gaggcggatt atctccacct
    29881 cctctggcgt tttgatttgc cgcagcacat ccggcgtggc gctaaccagt ttcgcattga
    29941 gttcagactt ccagcggtgc gcggtttccc aactcacctg ctggccttca aaaccgagcg
    30001 tttgcaactg ctcatcggca atgatttgat tgacgatagt ggtaagcgtg ttcgttgcgt
    30061 caagcaaatg cagctggtag ccttgcgcac gggcttccac ctcaacgaaa tagcgcgaat
    30121 ctaccagaat gtgcgcactt tcacggctaa taaccacata gcctgatccg gtggagatcc
    30181 ccagatgcgg ctgtttgttc tgccgtgagg agagaagcac cgcatccagt tgttgcacct
    30241 taagccagtt gcgcagcgaa gcgagtaatg tcatctggat tttccttctt acaggctatc
    30301 gattaacaat ttgcctttgc ggaacatcat cagacgcagg aacaccacca tcagcgcagt
    30361 aatgattgcc cccagcgcga tccccgccat ataaacgccc aagttagtga ccagcggcca
    30421 tgcccagata gcggattccg ggaaccattg cactgcgccc agccagacag ctgcggttga
    30481 gccgacaatc gcgccgacca tatatgacgg aatagcggtg atggggcttt ccagcgcaaa
    30541 cggaatcgca ccctcactaa tccccataaa ggccaggaac atcgcggtct taccctgcgg
    30601 ataaagttga gcgttgaaca ggcgtttgcc ggttaaacga cggtcaataa tggtcgccag
    30661 acccagacca atcggcggaa taacgatagc gatagaacga gcggtgaccg gtaatacgtg
    30721 gtcagtggta aagctgaagg caacgaaacc tgcggctttg ttgatcgggc caccaaggtc
    30781 gattgctgtc gccgcggcga tccccattgc gtacatcagc gcgcccttct cacctgcggc
    30841 agtcagcaca gtacggatac cgccgttgat ccagccacca aagggagtga tgacgtagta
    30901 catcgccagc atgacaaaaa tggcggaaag aatcggtagc aaaaatgtgg ttttgaacgc
    30961 taacaggaaa tccggtaact ggattttctg gttcatccat ttcacaaggt aaccggcaac
    31021 gatagaaata atcagcgcac caatgaaggt ggacggcacc ggcgaagagg tcgcccattg
    31081 catagtgctg ggatcgaagt tcagcagttg cgtcggctgg gtagacatca acccgccgat
    31141 aaaaccagcc gggaacgcca gtttgccacc gatagagttc gccacaaatg cagcaaacat
    31201 cggaatggca aaaccaaaca acacgccgcc gaaggactgc gacagccagg caaatttaag
    31261 taaggagagg tcgaaaccgg agaatttccc gctattaaga gcatccatga tgccgatctc
    31321 agcaggaatt ttaagccagc tataagcaat cagctggctg aaagcgagga tcacaccgcc
    31381 cataattaag gtcggcacca tgcgtgaaat ccctgacatc acatgctgcg ggagttcacc
    31441 ccagaagctg cttttagagg cctgcggatt cttaactgcc gctgccgcac cggatgcgcc
    31501 aggcacaacg gttgcactgc gttttttaat ggccataaca attatccctg tcgaatatta
    31561 ctgttgttca gaggcaatca tctcttcgat ttctttgatg atgcccgcag cgtttttaat
    31621 tgcgtcctgc aaagtgattt cataaacgtc gcgtgattcg aaacgttcgt tatcttccgg
    31681 ggtaactgcc acggaatgga tgatgatggt cgcttcggcg atatcctgcg ccgtcaggcg
    31741 attctggatg ccgtccgcgc cctgggtttc aattttcact tcataaccgg cttctaccgc
    31801 cgcttcttcc agcacctgag cggccataaa agtgtgagcc aggcccattg ggcaggcaca
    31861 taaggcaatc agtttcttac tcatcgttaa ttcctcgttc agtgatagtt gactagtgaa
    31921 attatgaacg tgcaaaacaa atcgccgtta ccagacaaat aatgcattta ctggagaatt
    31981 aatcccgctg tacggaagtg tgacgcaggt cggccttgtg aggggacaca aagaaaaatt
    32041 gaattaactg tatgatttaa aagattatcg ggagagttac ctcccgatat aacaggaagg
    32101 atttacagaa tgtgacctaa ggtctggcgt aaatgtgcac cggaaccgag aagacccgga
    32161 tagtcatgga cgatgagata caccggaata tcatggacat attctttaaa gcgcccttta
    32221 tcttcaaatg cggcacggaa accggaggct ttgaagaact caaggaagcg cggcacgata
    32281 ccgcccgcaa taaacacgcc gccaaatgtc ccgagattga gcgccagatt gccgccaaaa
    32341 cggcccataa tgacgcaaaa cagcgacaat gcgcggcggc aatcggtgca gctgtcagcc
    32401 agcgcgcgtt cggtaatatc ttttggcttg agattttctg gcaggcggtt gtcagctttc
    32461 acaattgcgc gatacaaatt caccagccca gggccagaaa gcacgcgctc cgccgaaaca
    32521 tgaccaattt ccgcacgcaa tatttcgagg ataatggcct cttcttcact attcggcgca
    32581 aaatcaacgt gaccgccttc gcccggcaag cttacccaac gcttatcgac atggaccaga
    32641 tgcgcaaccc caagccccgt tccggcaccg taaaccgcaa taggcttacc ttcgaccggt
    32701 tctgcgccac caaactgaat cagatgctct tttttcagca tcgggatcgc catcgataca
    32761 gcggtaaaat cgttaataat ttccagatgg ctaaaaccga gattcttttt catttcggca
    32821 attgagaacg cccaggtatg gttggtcatt gccacccagt caccggtaat tgggcaagcg
    32881 atggcaatac agccgtcttt cacctcgacc ttatgttctt caagataaac gcgaatgacc
    32941 gcttcgaggc tggggtaatc aagccctgaa taggtcttag cctgcgagat ttcaccactg
    33001 gcaatatcac acagagcaag acgtgcgttg gtgccgccca catcaccgac taatgcatac
    33061 tttgtcattc ttcaactgct ccgctaaagt caaaataatt ctttctcaca ctgtaaatac
    33121 ctgggggcat aacaacaacg ccggaaaggc aggctccctg taaatatcga tctgggtcac
    33181 acaattactt tatcgtttca gcaccaattg cagcgatgcc tttttgcaag ctgggcaaac
    33241 taagtatctg accccgcata aggaatagaa catgctccat ccgcgagcca gaaccatgtt
    33301 gttattatcg ctccccgccg tggcaattgg gattgcgtcc agtcttattc tgattgtggt
    33361 gatgaaaatc gcctcggtat tacagaattt gctctggcaa cgactgccgg gaactctggg
    33421 gatagcccag gattcacccc tctggatcat cggtgtatta acgctaacgg gtattgcggt
    33481 ggggttggtt atccgtttca gccagggtca tgccggacca gaccccgcct gtgaaccgct
    33541 gatcggcgca ccggttccgc cctctgcgct acctagactt atcgtagcat taattctcgg
    33601 tcttgctggc ggcgtcagcc tggggccgga acatccgatc atgaccgtca atatcgccct
    33661 tgccgtcgcc attggcgctc gtctgttacc gcgcgtcaac cgaatggagt ggactatttt
    33721 agcctctgcc ggaaccatcg gcgcgctgtt tggtacacct gttgcggcgg cgttgatatt
    33781 ttcgcaaacc ttaaatggca gtagtgaagt tccgctatgg gatcgtcttt ttgcgccgtt
    33841 aatggcggca gcagctggtg cacttactac cggattattt ttccatcccc atttttcact
    33901 gcccattgcg cattacggac agatggaaat gaccgatatt ctcagcggtg caattgtcgc
    33961 ggcgattgcc atcgcagcag ggatggtcgc cgtatggtgc ttaccacggt tgcacgcgat
    34021 gatgaatcaa atgaaaaatc cggtgctcgt gctgggtatt ggcggattta ttctcggaat
    34081 tctgggggtt attggtggac cagtttcgct gtttaaaggg ctggatgaga tgcagcagat
    34141 ggtggcaaat caggctttca gcaccagcga ttactttttg ctggcggtaa ttaaacttgc
    34201 cgccctggtc gttgctgccg ccagtggctt tcgcggtggg cgaatcttcc cggcagtgtt
    34261 tgtcggcgtg gcattagggt tgatgctgca tgagcacgtt cccgccgttc cagcggcaat
    34321 aaccgtttcc tgcgctattc tcggcatcgt gctggtggta acacgcgatg gctggttaag
    34381 tctttttatg gcggcagtcg ttgtacccaa taccacattg ctaccgctgc tctgtatcgt
    34441 catgcttccg gcatggctgt tattagcagg taagccgatg atgatggtca atcgtccgaa
    34501 gcaacagcca ccccacgata atgtttagca aaaaatgctc tcctttatga tttaagagtt
    34561 atggcgatga tacggggctt tacctccccg taatattgcg ttaacaggcc gctgacggct
    34621 tcgatcgtga aggagaataa caatgttcag gtcactgttt ctggcggccg ccctgatggc
    34681 atttaccccg cttgcagcaa atgcaggtga aatcacccta ctgccatcga tcaaattaca
    34741 aattggcgat cgcgatcatt acggtaatta ctgggacggt ggtcactggc gagatcgtga
    34801 ttactggcat cgcaattatg aatggcgcaa aaaccgctgg tggcgtcatg ataatggcta
    34861 tcatcgtggc tgggataagc gtaaagcata tgagcgtggc tatcgtgaag gctggcgcga
    34921 tcgtgacgat catcgcggaa aaggccgcgg acatgggcac cgccattaaa cgcgtcctcc
    34981 aatggagcac aatgcctgat gcgacgctag cgcgtcttat caggcctaca aaccggctct
    35041 ttaacgcgta gggcggataa ggcgtttacg ccgcatccgc caatagtgcc agatgcgacg
    35101 ctcattcaac tacaatccca gcgccgtccc caccaacaac cagatattca gcgccacgac
    35161 cagcactaca atcacccagc ctgtctgttt tacgcgtttg ctgttcacca gatcgcccat
    35221 caacttgctg tcactggtga aaatcagcag tggaaccagc gccagggcga taccaaaact
    35281 taacagcacc tgactcataa ccagaatccg tgtcggatct aatcccatca gaatgacaat
    35341 aaatgacggc aacatggtga ctgtacgacg cacccacagc gggatatgga agcgaatgaa
    35401 tccctgcatc accacctgcc ccgccagtgt ccccaccacc gttgaggaca gtccggcagc
    35461 aaccagactt aacccaaaga ccgttgccgc agcatggctt aacagcggtt gcagcgtcag
    35521 ataagcctca tcaagatcgg caacaccagt atgaccagaa aagtggaacg ccgccgcagc
    35581 tgtagccatc atcaccagat tgacaaaacc ggcaatcgtc atggcgatag ccacatccca
    35641 tttggtggcg gaataacgtt gttgacgcga accgccatgt aaatgctgag tgagcgaaga
    35701 gtgcaaataa atcacatgcg gcataatcgt cgcccctaac acgcctgctg ccaggaagac
    35761 cgcctccgaa gtaggtaaac tcgggatcac cattccttta cccagctgcg ccaggttagg
    35821 ctgggagaaa atcaactcga caatgtaagc cgcggcaaca aacaacagta acccgccaat
    35881 cactttctcc agcggttttt gcccgcgacg ttgcagcatt aaaatcagga aagtcgcgat
    35941 ccccgtcagc accgcgccct gcaacaacga aacaccaaga atgagtttaa aaccgatcgc
    36001 cgcaccaata aattccgcca ggtcggttgc catcgcaata atttctgcct gaacccaata
    36061 gaaccacact acgggacgcg gatagtgatc acgaatctgc tccgccagat ttttaccggt
    36121 ggcaatccct agtttggcag agaggatctg aatcagcatc gccatcaggt tggcccaaac
    36181 gacaacccac agtagctgat agccgaagct agcacccgcc tgaatattgg tcgcaaagtt
    36241 accgggatcg atataaccaa tcgccgcaat gaacgcaggt cccattaatg cgagcctcat
    36301 cttacgcgcc gcccgtccgc tgctactctc aacgcgatag ttcgtcatct tgtgcctcta
    36361 aaacatagcc tttgctatgt ttcatgctat gctaaacgag aatgattacc aaattcattt
    36421 aaatggattg tggtgatttc tctgatagac caagattatg attacgaaaa gattgatgac
    36481 ttgaatgtta tggttatgtt taatgttagc acatttacat aactttcagc ttccatacac
    36541 aacatagcag aaatgtatga cagatcacta tttttgaagc ctgtcacagg acgtcattat
    36601 agtgtgtgtc agatctcgtt ttccttaacc atgttacata gaatgtgcac ggaaatttaa
    36661 cctgcctcat atttggagca aatatggacc gcgtccttca ttttgtactg gcacttgccg
    36721 ttgttgtgat tctcgcactg ctggtaagca gcgaccgcaa aaaaattcgt atccgttatg
    36781 ttattcaact gcttgttatc gaagtgttac tggcgtggtt cttcctgaac tccgacgttg
    36841 gtttaggctt cgtgaaaggc ttctccgaaa tgttcgaaaa actgctcgga tttgccaacg
    36901 aagggactaa cttcgtcttt ggtagcatga atgatcaagg cctggcattc ttcttcctga
    36961 aagtgctgtg cccaatcgtc tttatctctg cactgatcgg tattctccag cacattcgcg
    37021 tgttgccggt gattatccgc gcaattggtt tcctgctctc caaagtcaac ggtatgggca
    37081 aactggaatc ctttaacgcc gtcagctccc tgattctggg tcagtcagaa aactttattg
    37141 cctataaaga tatcctcggc aaaatctccc gtaatcgtat gtacaccatg gctgccacgg
    37201 caatgtccac cgtgtcgatg tccatcgttg gtgcatacat gaccatgctg gaaccgaaat
    37261 acgtcgttgc tgcgctggta ctgaacatgt tcagcacctt tatcgtgctg tcgctgatca
    37321 atccttaccg tgttgatgcc agtgaagaaa acatccagat gtccaacctg cacgaaggtc
    37381 agagcttctt cgaaatgctg ggtgaataca ttctggcagg tttcaaagtt gccattatcg
    37441 ttgccgcgat gctgatcggc tttatcgccc tgatcgccgc gctgaacgca ctgtttgcca
    37501 ccgttactgg ctggtttggc tacagcatct ccttccaggg catcctgggc tacatcttct
    37561 atccgattgc atgggtgatg ggtgttcctt ccagtgaagc actgcaagtg ggcagtatca
    37621 tggcgaccaa actggtttcc aacgagttcg ttgcgatgat ggatctgcag aaaattgctt
    37681 ccacgctctc tccgcgtgct gaaggcatca tctctgtgtt cctagtttcc ttcgctaact
    37741 tctcttcaat cgggattatc gcaggtgcag ttaaaggcct gaatgaagag caaggtaacg
    37801 tggtttctcg cttcggtctg aaactggttt acggctctac cctggtgagt gtgctgtctg
    37861 cgtcaatcgc agcactggtg ctgtaaaacc atacataaaa aagccgggga tcattccccg
    37921 gcttttttac gcctgttaat cagctaatgg ctgcgggcga ccaatcaaat acccttgcag
    37981 atattgcacc ccgagcttat gcaatagcgc ctgctgctgt tgcgtctcga caaactccgc
    38041 gaccacactc aatgacttcg ctttcgccag atcggtaatt gatctcacaa tcatcgcatc
    38101 cagcgtattc gtgacaatat ctttcacaaa gacgccatca attttgatga tatcagcctg
    38161 caaacgcttt aaccgttcgt agttggcata tccggtgcca aagtcatcaa tcgcaatccg
    38221 gaaaccaaac ttatgcagct gctcgatgtt gtacatgctg ctttctgcgt tagaaaacgc
    38281 ctgctcctcg gtgatctcaa gaatgaccgc ctgcggggag atgtgataac gcttaaacag
    38341 acgaataatc cgcccggcaa tattcttttg cagcagcgtg agcggcatta aattgactga
    38401 aaagcgcggt ccttttttgt cgcaagggtg tgttgccagc cacttcaaca aggattccag
    38461 cacttgcaaa tcaaaacgcg cgctaaggtt aaactgagca ataaggggca gaaacttatc
    38521 cggggtcata atgccgccgt catatttcag tcgcgcgagg atctcatcat aaccttcacc
    38581 ctctttgttg cgaattggct gggcgtagag cagcaaatca ccctgatcta acgccgtgcg
    38641 aatggtattc agcaatagca cctgtttagt ggtctgcccg gaaaccatct cctctctgct
    38701 atccagcgcc agcacatgat gatgtgcgca ggattgctcc gccagccagc ttaactgccc
    38761 caacaagggt tgcagggttt cctgatttcc atcaaaacgc ccccaggcag caccatagcc
    38821 catatccagc ccggtattgt tccagtgaat ttgccgacta ttcaggatgt taaccatatg
    38881 ctggagtcgc ccttccgttt ccggcccgct cagcactaac agcagttcac tacccggcaa
    38941 ttgatacaac ttttcgtttt cctgcatcaa cggcagcagc gtacggcaaa ttgagcggat
    39001 acagtgaacg cgcatcatta agccgtaatg acgactcata aactcaagat tatcaatgcg
    39061 caggcagcaa aaactcttgc ccgcctcttg ctccggcgct tgttccaacg cacgaaagtt
    39121 gggtaggagc gttaacggat ctgttagcgc ctgcaaatgc cagcggcgat taagccattc
    39181 actgcgatga taaatgcgca ccatatagag caggcaaacg ctaaaggaaa tcagcaccgc
    39241 gagaataaat gccagcgaat attcggtttc caccccttgc aaaaagttct gattgtaatt
    39301 cagaaggcaa agcgttgaaa cagcccaggt gagatttaaa aacggatagc gaagattacc
    39361 gaccccgagg gtaaagatga tgaagaaaac gggtaccagg taaccggcaa taaagtcgtt
    39421 ttcataaggt gtgcacaaca gaagtagcag cacgcttaac gctgccagcc agcttaaggt
    39481 aaatgcgagt gagatagtag aaaagcatgt tgtaaatcag cactgcggtg aaaaggctta
    39541 gtaaatcaac gaccgtgaaa atggcatccg catcgccgaa aaaggtggat atctttagcg
    39601 ggaaatcaaa gaagctcccc acaagataca tgctgcattt gatgccgatc ggcgtcacca
    39661 aaccaagcca gaacagacgt tgccagacat atcggctggt caatccataa cgccaacgcg
    39721 tccccagctg ccagcgtaat attgcgcaag cacacaacac gacaaacgtc tggctgccga
    39781 gcatgaccgc agtctgtaaa aaagagagat gaaagttcca cagattggtg caaaacatcc
    39841 cgcataaaac gggaacgaca ccgcgccagc caaagataaa caagattgac agcattacgc
    39901 acagtggcat ccacgccaga aagatataac tggaatggat aatggcaagt ggcgaaataa
    39961 aacgggatag ctgaataagt accacggtga gcgtaaacgc tagtgtgaat atcttgatat
    40021 tttttatcag gttatgctcc acaaacatga gtacactact ctcactgatc ggggttttca
    40081 ggcacacaaa taataagttt attcgcatcg cgaagcaaac aggggccagc ctgttgccga
    40141 tttttcatcc aatgatgctt tcaccaggcg gcgctaaccc ggacgtagac tgtattacaa
    40201 aagcggcaaa aagcagagac aaaaaacccc cgctttgcag cgagggttgg aaatttggtg
    40261 gagctaagcg ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga
    40321 gctatagccc cacgatgcgt ttacgtacca aatttgctgg gtgcaaaatt tggtggagct
    40381 aagcgggatc gaaccgctga cctcttgcat gccatgcaag cgctctccca gctgagctat
    40441 agccccgtca cgtaaagctt gtcgagttga cgggcggcat catatgaatt ccgcccgaat
    40501 gtgtcaacgg caaattgcaa cgtgtagttt caatcgctga aaaatcaggc aaatgaacaa
    40561 ttttggaaaa ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt aaagtggtgt
    40621 tataaaatga actactaata gacccacata cattcaggga attgttatgt tcaaggagag
    40681 gatgacacca gatgaacttg ccagactgac cggctatagc cgccagacca ttaataaatg
    40741 ggtacgcaag gaaggctgga caacgtcacc aaaacctggc gtccagggtg gcaaagcccg
    40801 actggttcac gtcaatgaac aggttcgtga atatattcgc aatgctgaac gtccagaagg
    40861 tcagggagaa gcgcctgccc tttctggtga tgcgccgctt gaagtattgc tggtgacact
    40921 ggcgaaagag atgacgccag ttgaacaaaa acagtttaca tccttgcttc tgcgggaagg
    40981 gattatcgga ttgttacaac gcttagggat tcgcgatagc aaataatatg aaaagattac
    41041 gcaataaaat gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa caaactgtta
    41101 accgttggac cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg aaaggcggtc
    41161 gtgccagact tattctggtc gatacgcaag tttgcgagtt tattcagaac acgccagcct
    41221 tccataacac accaatgttg atggaagctg aagagagaat tgctgaatac gctcctggag
    41281 cacgcgcccc ggcgtatcga caaatcatca acgcgataga taatatgaca gacattgagc
    41341 aggaaaaagt cgcgcaattt ttgtcacgtg aaggaattcg caatttcctc gcccgtctcg
    41401 atattgacga atcagcataa ataaaaaacg gcaggatatt atctcctgcc gtttatcttt
    41461 ttacacgcta attactgctg attttcgcgt tcagcaataa aatccagcgc tttgttgata
    41521 cgctcgatac tgcgggtctt accaattgcg tgaacggtaa catccagtgc tggagactgc
    41581 cccgcaccgg ttacggcgac acgcagcggc ataccaactt tacccatacc cacttccagc
    41641 tcatccgccg tcgcctgaat agcgtgatga acgttttcag cggtccagtc agtaatcgcg
    41701 gccagtttgt cacgaaccac ttccagcggc tgacgcgcta ccggacgcag atgttttttc
    41761 gccgcatcgg catcgaactc agcaaaatct tcgtagaaat aacggcagct ctgtgccatc
    41821 tctttcagcg tcttgcagcg ttcgcccagc agtttcacca gatcagccag ctgcgggccg
    41881 ttacgggtat cgatattttc ctgctcaatg tgccactgta agtgagtagc aacatactcc
    41941 ggcggcagcg cgttaatgta gtgatggttc agccacagca gcttgtcggt gttgaacgca
    42001 ctggcagatt tgctgacggc attcaaagtg aagtatttga tcatctcttc acgagtgaag
    42061 atttcctgat cgccgtggga ccagcccaga cgcaccagat agttcagcag tgcttctggc
    42121 aaataaccgt catcacgata ctgcattacg ctgactgccc cgtgacgttt ggacagtttt
    42181 ttaccgtcat cgccattgat catagaaacg tgcgcgtaaa ccggcaccgg cgctttcagg
    42241 gccttaagaa tgttgatctg gcgcggcgtg ttgttgatat ggtcttcgcc acggataacg
    42301 tgggtgattt ccatatccca gtcatcgaca accacacaga agttataggt tggggaacca
    42361 tcggtacggc ggataataag atcgtccagt tcctggttgc tgaactcgat cggaccacgg
    42421 atctgatcgt caaaaacaac agaaccttcc tgcgggttag caaaacgtac aacacacggt
    42481 tcatcatcag catgatgctc atggctgtgg cggcagcgac cgtcataacg cggcttctca
    42541 cctttcgcca tttgctcttc gcgcagcgct tccaggcgct ctttagagca atagcattta
    42601 taagcagtgc cctcttccag catctgatcg atcaccgcgt tgtagcgatc aaaacgtttg
    42661 gtctggtagt acggaccttc atcccactcc aggctcagcc agttcatgcc atccataatg
    42721 gcttcgatag cttccggcgt ggaacgctca agatcggtgt cttcaatacg cagcacgaac
    42781 tcaccgccgt ggttacgtgc aaaaagccag gagtaaagag cagtacgcgc gccgccaacg
    42841 tgcagatagc ctgttgggct tggcgcgaag cgagttttga ttttcatgaa atggccttac
    42901 gtttagaaag atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg atattctatc
    42961 actcccgcct gattcctcaa tgtagttcgg gtttctacct tacgcattat tgttttcgtt
    43021 gacaaattgc gcaacacggt ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa
    43081 caatttcttt agaaaatgcg ttgactcatt ttgaactctc cctataatgc gactccacac
    43141 agcgggggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    43201 cgatcccgtc atcacccacc aactacttta tgtagtctcc gccgtgtagc aagaaattga
    43261 gaagtgggtg attagctcag ctgggagagc acctccctta caaggagggg gtcggcggtt
    43321 cgatcccgtc atcacccacc acttcgggtc gttagctcag ttggtagagc agttgacttt
    43381 taatcaattg gtcgcaggtt cgaatcctgc acgacccacc aatgtaaaaa agcgccctaa
    43441 aggcgctttt ttgctatctg cgatactcaa agattcgaac ctgcagcagg tttgagttga
    43501 gcgcagcgaa acaacggagc cgctcgcggc gacggcccga agggcgagcg aagcgagtca
    43561 tcctgcacga cccaccaatg caaaaaagcg ccctaaaggc gcttttttgc tatctgcgat
    43621 ttgtaagatt gatttttctt ctcaagttcc ttgttcttct accgatatta cctcgtacag
    43681 tgacgaggac tgaacaatgg ccactatctc ttcaacttca attccttcca tacaaacaca
    43741 gagtagtaac agggcatcac aaggtagcga tgttgcttca caaatcgcgc ggataagtca
    43801 gcaaatcatc aaactcactc aacaaattaa agagattgta gacactagcg gcagtgctga
    43861 agacaagcaa aaacaggccg aattgattca acagcaaatc accctactgg aaacacaact
    43921 ggcgcagttg cagaaacagc aggcagaaaa ggcgcaggag aaagagcagc gtttgtctct
    43981 gaatgtatcc cttctaaatc ctgttgaaaa aacgactcat atcgatatct atatctgata
    44041 caacggatcc ccttcccgac cagccattag cgcattgccc tggtctcgta ccacgtccca
    44101 taacgcttca gcggcggtgg aaagtgaacg gttcttcctg cgcaccagca tcaactggcg
    44161 ttcaaccacc ggcgtaatgc gcttaaccac cagtgggcta ccttcaggca atggtagcgc
    44221 cagtgcagga agaatactga tgccgatgcc tgcggctacc atcgggaaca gcgtcgccgg
    44281 atgtccaatc tcctgcacaa tattcgcctg aataccattg cgcgccagcg ctgcgtcaat
    44341 cagcggtcgg ctgcctgacg cgtaatcctg caacaccaat ttcgctccct gtaatgcctg
    44401 ccagggtaca taatcttcca cggccaaagc gctatcgcga tggcaaagca gaaagaaagg
    44461 ctcggaaaga atcgcttcac attgcagatc gcccactggg cctggatcga tgacgatgcc
    44521 aaagtcgaca tctccctggc gaatactttc catcacccac tgctgcggac gatcatgcag
    44581 gacaaactga atatctggat agcggcgatg gctttcagca atgcattgcg gaataagatg
    44641 cgcggaaatg gtctggctgg cagcgacccg aacttttccg ctcagttgtt gccccatacg
    44701 cccggtatcg cgcaacgtgc tgttaagttc atccagcagt cgctcaagac gcaaagccaa
    44761 ctgctgccct gcatctgtaa gcaccacttc acgcgtggtt ctgtccagca ggcgaacacc
    44821 agtatgattt tccagctcct tcacactgtg acttactgcc gactggctca agccgatacg
    44881 ctctcctgca cgactaaaac ttttctcctg cgctactgtg acgaaaacct ttaattgttt
    44941 taaagaataa ttcatctgtt ttcttcatga atggatgcaa taaatcaatt ttatttctca
    45001 aactggaaga agcacaatag aaccatcgat catctggagt ctttatgaaa ctttttcgta
    45061 tcctcgatcc tttcacctta accctgatca cggtggtgtt gctggcctct ttctttccgg
    45121 ccagaggcga tttcgtcccc ttctttgaaa atctgaccac cgcagctatt gccctgctgt
    45181 tctttatgca cggcgcgaag ttgtcgcgtg aggcgattat tgctggcggt ggtcactggc
    45241 gactgcattt gtgggtaatg tgcagcacct tcgtgctgtt tccgattctg ggtgtactgt
    45301 ttgcctggtg gaaaccggta aatgtcgacc cgatgctcta ctccggtttt ctctacttgt
    45361 gcattctccc ggctaccgtg cagtctgcaa tcgccttcac gtcaatggcg ggcggtaacg
    45421 tcgcggcggc ggtttgttct gcgtcagcat ccagcctgct ggggattttc ctttcaccat
    45481 tgctggttgg tctggtgatg aatgttcacg gtgcaagggg cagccttgag caggtcggta
    45541 aaattatgct gcaactgctg ctgccgtttg tgttggggca tctttcccgg ccgtggattg
    45601 gtgactgggt gtcgcgcaat aaaaaatgga ttgcgaaaac tgaccagacg tccattctgt
    45661 tggtggttta tacagcgttc agcgaagccg tcgttaatgg tatctggcat aaagttggct
    45721 ggggatcatt gctgtttatc gtggtggtca gctgcgttct tctggctatc gtgattgtag
    45781 ttaacgtctt tatggcacgc cgactgagct tcaataaggc agatgaaatt actatcgtct
    45841 tttgtggttc gaaaaagagt ctggcaaatg gcatcccgat ggcaaacatt ctgttcccca
    45901 catcggtgat cggtatgatg gtgctgcccc tgatgatttt ccatcagatc caattgatgg
    45961 tctgtgcggt gctggcgcgt cgatacaaac gccagaccga acagttacag gcgcagcagg
    46021 aaagcagcgc cgataaagct taaagcggac gcttcagggg ctggaccagt tgcgtcagcc
    46081 cctcggtttt aatcagcagc gtgatttgca ttagctcacc gagcttccct gccgggaact
    46141 catctttgcg ggcaaaccac agcagatact cctccggcag gtcaattaag cgacgccctt
    46201 tgtatttgcc aaacggcatt atcgtattgg ctatttcaat cagctgctct ttttccatct
    46261 cagctaccca gcaaacgcag catttccgct tcgtcgatga cttcaatgcc cagttcctgc
    46321 gccttcgcca gtttagatcc tgcagcttca cccgctatca ccagatcggt tttcttcgac
    46381 acgctgcccg cgactttcgc gcccagttcg accagtcgag ctttagcgtc atcacgcgac
    46441 atctggctta agctgcccgt aagcaccacg gttttaccag caaacgggct gtcaatctct
    46501 tccgcgttga taacgatcgg cgcaggccag tgaacacctt ccgccaacag ctcgctgatg
    46561 acattgcggt tgctttcttc ggcaaagaag ttgtgaacgt gggatgcaac gacaatgcca
    46621 acatcaggca ccttttgcag ctcttcaatc gaagcggctt ccagcgcttc cagcgtgccg
    46681 aaatatgccg ccagacctgc tgcggtggcc tcgccgactt cacggatgcc aagtgcatag
    46741 aggaagcgag caaaggtggt ttctttcgct ttttccagcg cgttaaccac gttttgtgcc
    46801 aattttggcc ccatacgctc cagtccggtc agtttgcctg cggtgagttt gaacagatct
    46861 gccggagtat ggacatattc tttttcaacc agctgatcga tgattttgtc gcccattccg
    46921 tcaacatcca tcgcacggcg ggaaacaaag tgtttcagcg actctttacg ctgcgcaccg
    46981 caaatcaggc caccggtaca gcgggcaacc gcttcacctt ccacacgctc aacgtcagaa
    47041 ccacataccg gacaatgcgt cgggaataca acctcacggg tatcttccgg gcgttcagaa
    47101 agcacgacgt taaccacctg cgggatcacg tcgccagcgc ggcgaatcac cactttatcg
    47161 ccaatgcgta aaccaagacg ttcgatttca tccgcattgt gtaaggttgc gttactcacc
    47221 agcacgccag caacatggac aggttccaga cgcgcaacag gcgtaatcgc gccagtacgc
    47281 ccaacctgaa actcgacgtc acgcacaaag gtcatctgct cctgcgccgg gaatttaaac
    47341 gcaaccgccc agcgcggggc acgcgcgaca aagccaagct gctcctgctg ttccagtgag
    47401 ttgaccttaa tcaccacgcc gtcgatatca aagcccagcg tcgggcggtc ttcttccact
    47461 ttgtgataga acgccagcac ttcttccgcc gattcacaaa gcgttacccg atcgctgacc
    47521 ggcacccccc actttttaaa ttgcagtaaa cggccaagat gagtatccgg cagctcgcca
    47581 ccttccagaa caccaacgcc atagcagaaa aaagtgagcg gtcgcttcgc tgtaatacgc
    47641 ggatcaagct gacgcagtga accagctgcc gcattacgtg ggttagcaaa aactttcccg
    47701 cccgtgcgtc gcgcatcttc gttaatcttt tcgaaccccg cctgcggcag gaacacttca
    47761 ccacgcactt ccagacgcgc cgggatattc tctccgtgca gcttcagcgg aatggcgcga
    47821 atagtacgca cattagacgt gatatcttcc ccggtggtgc catcgccacg ggtcgcggca
    47881 ctgactaaaa cgccattttc atacagaata ctgacggcaa ggccatccag cttcagctca
    47941 cagcaccagg tgactttctc gttgtttttc agacggtcct gcacacgttt gttgaaagca
    48001 agaaagcttt cttcatcaaa aacgttatcc agtgacagca ttggtacttc atggcgtatc
    48061 tggctgaaag ctgccagcgg cgcagcacct acacgttggg taggcgaatc aggcgtaatc
    48121 agttctggat gtttggtttc cagctcgcgc agttcgcgca tcagcctgtc gtattcagcg
    48181 tcgggaattt ccggcgcatc catcacatga taaagatatt catgatggca aagcgtcgtt
    48241 cgcagttctg tcagttgttg ttcgattgat tccatatcgc accatcaatg ctaaaaaccc
    48301 ccgacaagcg ggggttcgaa gaggagttaa tttgccttaa gtgtatcagg cgttggcgtc
    48361 tttgacttcg cggatgatgt cctggtactc gcgcaatttc tgcggagtca tcatacggcg
    48421 ctgatcgtca agcacgacac cgcccacttc atcggcaata tgctgcgcag attgcagcat
    48481 cagcttgaag ttctgcagct cgtcaccgta agacggtacc tgcataaaga tagtgacacc
    48541 cggagtagtg aaatccttca tttcaggatc aaaggttccc ggtttcacca tattcgccag
    48601 gctgaataac gccgggccgc tgccatccgg gctaagatga cgatggtaaa tattcatatc
    48661 gccaaaaatg aagcccgctt gttgaatgct gttaagaagc agttcaccgt ttagctcgct
    48721 accgtgatgc gccgcgacgt tcatgataat caccgcttct ttgcgcttcg gtttatccat
    48781 aactggagcc ggttccgcta caggctcagg ctgtggtgcc gctacgggtt ctgcaggctg
    48841 gaaagcctgt tgtgccggtt gcggtgctga atgcacaggc tgcggcgctg gcgcgacctg
    48901 tggcgaaact ggctgctgca acggctgttc aggctgcggc tgataggcag gctgctgcac
    48961 cggctgcgct ggacgcggag catgttgcgg cggtacctgc gcttcaggcg gctgctggac
    49021 cggttgacgc ggctgcgcag acgcataagg cggtaggtac tggtgttgcg gcgacggacg
    49081 agcagcctca tgctcctgag cgttagccgg ggcatgattc acgcggtgaa cacgaacctc
    49141 accaacgccc tcatcatctt cgacatcctc gtcataagaa tcgtcgtcac gttttgactt
    49201 cattcgtttt aatggccgat cgcggaacat agaagatcgt tctttacggc tggtccagaa
    49261 accatgtacc agtaaagcga ttatggcgat cgcgccaaca atgattaata tcagacgcaa
    49321 atcctgcatc attatattct ctgttgttct aacaccttgc caccacggca aacatttact
    49381 cactaagagt atttgccgat tacctcaagt gcaagtgcac tattaacttt cacagcacaa
    49441 agatagatga aatcgtgctt tttgctgttt tttcgaacat atcctaactg tccattgcgc
    49501 aattacccgg taaaatacgc agaattttcc tgggattggt caaaaggagc tcatcctgat
    49561 tatggtttca tcattcacat ctgccccacg cagcggtttt tactattttg cgcaaggctg
    49621 gaagctcgtc tcgcaacctg ggattcggcg tttcgttatt ttaccgctgc tggtcaatat
    49681 tttgttgatg gggggcgcat tctggtggct ctttacacag ctcgatgtct ggatcccgac
    49741 tttcatgagt tacgttccgg actggctaca atggctgagt tatctgttgt ggcctctggc
    49801 tgtcatctct gtgctgttag tgtttggcta tttcttctcc acgattgcta actggattgc
    49861 cgctccgttt aacggtttat tggctgaaca actggaagca cgattgactg gcgctacacc
    49921 gccagatacc gggattttcg gtatcatgaa agatgtgccg cgaatcatga aacgcgaatg
    49981 gcaaaaattt gtctggtatc tgccgcgcgc aattgtatta ctaattcttt acttcatccc
    50041 tggtattggg caaaccgtcg cgccggtact gtggttcctg tttagcgcct ggatgttagc
    50101 catccagtat tgcgattacc ccttcgataa ccacaaagtg ccgtttaaag agatgcgcac
    50161 cgccctgcgc acacgcaaaa tcaccaatat gcagtttggt gctttaacca gcctgtttac
    50221 gatgatcccg ctgcttaatc tgttcatcat gcccgttgcc gtttgtggcg cgacggcgat
    50281 gtgggtcgat tgctatcgcg ataaacacgc gatgtggcgg taacaatcta ccggttattt
    50341 tgtaaaccgt ttgtgtgaaa caggggtggc ttatgccgcc ccttattcca tcttgcatgt
    50401 cattatttcc cttctgtata tagatatgct aaatccttac ttccgcatat tctctgagcg
    50461 ggtatgctac ctgttgtatc ccaatttcat acagttaagg acaggccatg agtaagattt
    50521 ttgaagataa ctcgctgact atcggtcaca cgccgctggt tcgcctgaat cgcatcggta
    50581 acggacgcat tctggcgaag gtggaatctc gtaaccccag cttcagcgtt aagtgccgta
    50641 tcggtgccaa catgatttgg gatgccgaaa agcgcggcgt gctgaaacca ggcgttgaac
    50701 tggttgaacc gaccagcggt aataccggga ttgcactggc ctatgtagct gccgctcgcg
    50761 gttacaaact caccctgacc atgccagaaa ccatgagtat tgaacgccgc aagctgctga
    50821 aagcgttagg tgcaaacctg gtgctgacgg aaggtgctaa aggcatgaaa ggcgcaatcc
    50881 aaaaagcaga agaaattgtc gccagcaatc cagagaaata cctgctgctg caacaattca
    50941 gcaatccggc aaaccctgaa attcacgaaa aaaccaccgg tccggagatc tgggaagata
    51001 ccgacggtca ggttgatgta tttattgctg gcgttgggac tggcggtacg ctgactggcg
    51061 tcagccgcta cattaaaggc accaaaggca agaccgatct tatctctgtc gccgttgagc
    51121 caaccgattc tccagttatc gcccaggtgc tggcaggtga agagattaaa cctggcccgc
    51181 ataaaattca gggtattggc gctggtttta tcccggctaa cctcgatctc aagctggtcg
    51241 ataaagtcat tggcatcacc aatgaagaag ccatttctac cgcccgtcgt ctaatggaag
    51301 aagaaggtat tcttgcaggt atctcttctg gagcagctgt tgccgcagcg ttgaaactac
    51361 aagaagatga aagctttacc aacaagaata ttgtggttat tctaccatca tcgggtgagc
    51421 gttatttaag caccgcattg tttgccgatc tcttcactga gaaagaattg caacagtaat
    51481 gccagcttgt taaaaatgcg taaaaaagca cctttttagg tgcttttttg tggcctgctt
    51541 caaactttcg cccctcctgg cattgattca gcctgtcgga actggtattt aaccagacta
    51601 attattttga tgcgcgaaat taatcgttac aggaaaagcc aaagctgaat cgattttatg
    51661 atttggttca attcttcctt tagcggcata atgtttaatg acgtacgaaa cgtcagcggt
    51721 caacacccgc cagcaatgga ctgtattgcg ctcttcgtgc gtcgcgtctg ttaaaaactg
    51781 gcgctaacaa tacaggctaa agtcgaaccg ccaggctaga ctttagttcc acaacactaa
    51841 acctataagt tggggaaata caatgttcca gcaagaagtt accattaccg ctccgaacgg
    51901 tctgcacacc cgccctgctg cccagtttgt aaaagaagct aagggcttca cttctgaaat
    51961 tactgtgact tccaacggca aaagcgccag cgcgaaaagc ctgtttaaac tgcagactct
    52021 gggcctgact caaggtaccg ttgtgactat ctccgcagaa ggcgaagacg agcagaaagc
    52081 ggttgaacat ctggttaaac tgatggcgga actcgagtaa tttcccgggt tcttttaaaa
    52141 atcagtcaca agtaaggtag ggttatgatt tcaggcattt tagcatcccc gggtatcgct
    52201 ttcggtaaag ctctgcttct gaaagaagac gaaattgtca ttgaccggaa aaaaatttct
    52261 gccgaccagg ttgatcagga agttgaacgt tttctgagcg gtcgtgccaa ggcatcagcc
    52321 cagctggaaa cgatcaaaac gaaagctggt gaaacgttcg gtgaagaaaa agaagccatc
    52381 tttgaagggc atattatgct gctcgaagat gaggagctgg agcaggaaat catagccctg
    52441 attaaagata aacacatgac agctgacgca gctgctcatg aagttatcga aggtcaggct
    52501 tctgccctgg aagagctgga tgatgaatac ctgaaagaac gtgcggctga cgtacgtgat
    52561 atcggtaagc gcctgctgcg caacatcctg ggcctgaaga ttatcgacct gagcgccatt
    52621 caggatgaag tcattctggt tgccgctgac ctgacgccgt ccgaaaccgc acagctgaac
    52681 ctgaagaagg tgctgggttt catcaccgac gcgggtggcc gtacttccca cacctctatc
    52741 atggcgcgtt ctctggaact gcctgctatc gtgggtaccg gtagcgtcac ctctcaggtg
    52801 aaaaatgacg actatctgat tctggatgcc gtaaataatc aggtttacgt caatccaacc
    52861 aacgaagtta ttgataaaat gcgcgctgtt caggagcaag tggcttctga aaaagcagag
    52921 cttgctaaac tgaaagatct gccagctatt acgctggacg gtcaccaggt agaagtatgc
    52981 gctaacattg gtacggttcg tgacgttgaa ggtgcagagc gtaacggcgc tgaaggcgtt
    53041 ggtctgtatc gtactgagtt cctgttcatg gaccgcgacg cgctgcccac tgaagaagaa
    53101 cagtttgctg cttacaaagc agtggctgaa gcgtgtggct cgcaggcggt tatcgttcgt
    53161 accatggaca tcggcggcga caaagagctg ccatacatga acttcccgaa agaagagaac
    53221 ccgttcctcg gctggcgcgc tatccgtatc gcgatggatc gtaaagagat cctgcgcgat
    53281 cagctccgcg ctatcctgcg tgcctcggct ttcggtaaat tgcgcattat gttcccgatg
    53341 atcatctctg ttgaagaagt gcgtgcactg cgcaaagaga tcgaaatcta caaacaggaa
    53401 ctgcgcgacg aaggtaaagc gtttgacgag tcaattgaaa tcggcgtaat ggtggaaaca
    53461 ccggctgccg caacaattgc acgtcattta gccaaagaag ttgatttctt tagtatcggc
    53521 accaatgatt taacgcagta cactctggca gttgaccgtg gtaatgatat gatttcacac
    53581 ctttaccagc caatgtcacc gtccgtgctg aacttgatca agcaagttat tgatgcttct
    53641 catgctgaag gcaaatggac tggcatgtgt ggtgagcttg ctggcgatga acgtgctaca
    53701 cttctgttgc tggggatggg tctggacgaa ttctctatga gcgccatttc tatcccgcgc
    53761 attaagaaga ttatccgtaa cacgaacttc gaagatgcga aggtgttagc agagcaggct
    53821 cttgctcaac cgacaacgga cgagttaatg acgctggtta acaagttcat tgaagaaaaa
    53881 acaatctgct aatccacgag atgcggccca atttactgct taggagaaga tcatgggttt
    53941 gttcgataaa ctgaaatctc tggtttccga cgacaaaaag gataccggaa ctattgagat
    54001 cattgctccg ctctctggcg agatcgtcaa tatcgaagac gtgccggatg tcgtttttgc
    54061 ggaaaaaatc gttggtgatg gtattgctat caaaccaacg ggtaacaaaa tggtcgcgcc
    54121 tgtagacggt accattggta aaatctttga aaccaatcac gcgttctcta tcgaatctga
    54181 tagcggcgtt gaactgttcg tccacttcgg tatcgacacc gttgaactga aaggcgaagg
    54241 cttcaagcgt attgctgaag aaggtcagcg cgtgaaagtt ggcgatactg tcattgaatt
    54301 tgatctgccg ctgctggaag agaaagccaa gtctaccctg actccggttg ttatctccaa
    54361 catggacgaa atcaaagaac tgatcaaact gtccggtagc gtaaccgtgg gtgaaacccc
    54421 ggttatccgc atcaagaagt aattcttgcc gcagtgaaaa atggcgccca tcggcgccat
    54481 tttttatgct tccgccagcg gcggcaaaat caattcatcg ctctcatgct gctgggtgta
    54541 gcgcatcact tccagtacgc gcaaccccgc acggtgcacc gcatcgttta acgccttccc
    54601 tttcagcaaa ccactgataa gctgagcaca aaacaggtcg ccagtccctt tcaggtcggt
    54661 ttttacccgt gaatgggaaa tgacattcac gctgtcggca ctgaccacca ccacctgcat
    54721 ctcctgattt tcttcattac cggaggcgct ggtaatcacc acccatttta atgtgtctga
    54781 aagcagactt tttgcggcag caatggcact gtcgagatcg cggcaatttt taccggtcag
    54841 gatttccagc tcaaagatat tgggggtaat tccctgcgcc agcggcagta aatattgtcg
    54901 atacgcctcg ggaaggtcag gtttgacata aattccgcta tcaatatcgc caatcaccgg
    54961 atcgaccatg atcaataggt caggatggtc tttgcgtagc gcagtcagcc actcggcaag
    55021 gattttgatt tgcgatgccg ttcccatata gcccgtggtt acagcacgaa gttggcgcag
    55081 cgcatcacgc tcctgaagcg cacgcaaata gccgctaaac cattcgtccg gaatcgcacc
    55141 accgtagaaa gtgtcataat gcggcgtatt gctcagcaat accgtcggca cggcaaagac
    55201 attcaagccg ttctgtttta tagcaggcac cgcaatgctg ttgcctacgc ttccgtacac
    55261 tacctgcgac tgcacggcga cgatatccgc ctgcagcgcc ctactcttat cgttaaacaa
    55321 caacaaacta ctcatttaat tttttctcct tgccgatgat cctcatcgta ttccaaccga
    55381 aactttacct gattctggca gtcaaatcgg ctattacaaa acaaagataa ggtaattcag
    55441 tgaagaaaat catttgtctg gccattacgc tgctaatgac actccccgct tacgcgaagt
    55501 taactgttca tgaagaagcc cgcatcaacg ccatgctgga gggattagca cagaaaaagg
    55561 atttgatatt tgtgcgcaac ggtgatgaac atacctgcga tgaagcggtt tcccacctgc
    55621 gtctgaagct cggcaatacc cgtaaccgca ttgacactgc cgagcagttt attgataagg
    55681 tagcttcgtc gtcatcgatt accgggaagc cgtatatcgt gaagatcccc ggtaagagcg
    55741 atgagaacgc acagcctttt ttacatgcgt taattgcgca gacggataaa acggtgcctg
    55801 cggaaggaaa ttaatccgct ttggtaaggc atttacagga ggtaacatga aaaaatgctt
    55861 tatttatcac gatgaaaaat cgaataaatt ttggtggata gattacgaag gggatagttt
    55921 agctgtcaac tatggcaagg taggtagtat tggtaaattc catacaaaag aattcgataa
    55981 tgaagaacag tgtctgaaag aagccagtaa attgattgcc gcaaaaatga aaaaaggata
    56041 tcaagaaact ccagacttta acttcatgga tctctactat tttgatgatg aagagattgg
    56101 gttacatctt aaaacgtcac acccaaactt ccagtgccat tttactgatc cactttatat
    56161 gtgttgctgg gatgaagaat ctccttttgg tagcgatgaa ggtgctgatg ctctaaacga
    56221 acttgaaaat agcctccgtt ctggactgtg ctgatttccc tcaaatgtta attgaaacta
    56281 tgtggggtat gaaatacatc gctatggaca gtattcttga agaggatgtg cgtgcgcaat
    56341 tactagccga tgaaatgagc tctatccaga gcaatatgat tacctacgcg actgcattcg
    56401 gtcagatcaa agtcatgggt aaaatctccc ataaacttaa aaagatggga ctcaatgcac
    56461 tagcgcgtca tcagcttacc gcaaaaattc ttcaatgggg tgacggtcag gactcaccaa
    56521 tacttcaaaa aatgattgat gaccttactg cttttccaca cgaaaattaa aaactgcatt
    56581 tgtcggcagc aacaactgcc gacaaatcag attattgaca aagtgcactt tgtttatgcc
    56641 ggatgcggcg taaacgcctg atccggcata aaaatattgc taattcaata tattgcagaa
    56701 acattgtagg cctgataagc ggagcgcatc aggcagtttt gcgtttgtca tcagtctcca
    56761 atgctattaa tccttaaatc cctgccccct gactaaaatg ctcttcccca aacactccgg
    56821 tggagaggta gcgatcgcca cgatcgcaga tgatcgctac caccaccgca ccagggttag
    56881 cttttgccac ccgcagtgct ccagcaaccg cgccgcctga gctgacgcca cagaatattc
    56941 cttcccgcac cgccagttcg cgcatggtgt tttccgcatc gcgctgatga atatccagca
    57001 cctcatccac cagcgcagcg ttgaaaatcc ccggcagata ttccgcaggc cagcggcgaa
    57061 tgcccggaat actgctgcct tcttccggtt gcaggccaac aatggtcacc ggtttggatt
    57121 gttcgcgcat aaagcatgaa acgccggtga tagtgccggt cgtccccatg ctggagacaa
    57181 aatgagtgat gcgcccgccg gtttgctgcc agatttccgg cccggtggtg gtgtaatgcg
    57241 cataagggtt atcgggatta ttgaactgat cgagcagctt tccttcgcca cgattcgcca
    57301 tctccagcgc cagatcgcgc gcaccttcca tgccctgctc tttggtgaca agaatcagtt
    57361 ccgcaccata agcacgcatc gccgcacggc gttcctggct catgttgtcg ggcatcagca
    57421 atttcatgcg atagcctttc agcgcggcaa tcattgccag cgcaatgccg gtgttaccac
    57481 tggtggcttc gattaagaca tcacccggtt taatttcccc gcgcttttcc gcctcgacga
    57541 tcatcgaaag tgccgcacga tctttcaccg aacctgccgg gttattgcct tccagtttta
    57601 accacacttc actgccgtta tccggcccca ttcgctgcaa cttcaccaga ggcgtattgc
    57661 ctattgtttg ttctaatgta ctcacaatct ctatccatac gtggtgttgc ctgatgcgac
    57721 gcttgcgcgt cttatcaggc ctacaggtta caaaccttgc cattaaaaag cccggatcgc
    57781 gggaagcgcc tccgggcgtt taacattcac tcaacctatc aggcgctttg tgcgagagca
    57841 agttcctcat cgcgggtttc aatacgctcg tcgccgttat acagccgcgc atgttgcaga
    57901 ccaacgaata aacgctcgcc atgctgcggg gcatcgtcgc catgcatcac gaccgtcagc
    57961 ggttcgttgt accaccccag cggctgcacc actaattggg tgtagtgacc tttcgggctg
    58021 gcttccagta cctgtaccgg cagcggcgaa tcgagactgg tacggcggct gatatccact
    58081 tcccacgggc gcaggaagag atccaccggc ccctgatacg caggtgtgta gcccagcggc
    58141 cagcgatgcg cgccaacatg gaactgcccg ccgcgaatgg ttccctgcag gcggttcact
    58201 tcgcccataa attcgagtac aaaacgggtc gctggttcgc gccatacctg attcggcgca
    58261 tcagcctgtt caatattccc ctgactcatc accactacac gatcggctac ttcggtcgcc
    58321 tcttcctggt cgtgggtcac aaacacgctg gtgaatttca gctcttcatg cagttgacgc
    58381 agccagcgac gcagctcttt acgcacctgc gcatccagcg cgccgaacgg ttcatcaagc
    58441 agcagaattt gcggttctac agccagcgcg cgcgccagcg ccacacgctg tttctggccg
    58501 ccggaaagct gcgccggata acgatccgcc agatgcgcaa gctggaccat ttccagcaat
    58561 tttgtcactt tcgctttgat ggctgcggca ttcgggcgct cgcgacgcgg cagcaccgtc
    58621 aggccaaaag tgatattgtc gaataccgtc atatggcgga acagcgcgta atgctggaac
    58681 acgaaaccga ctttacgatc gcgcgcgtgc aggcgactca cgtcggtgcc gtggaagcga
    58741 atatgcccgc tggtttgatg ctccagcccg gcgataatcc gcagcagcgt ggttttcccg
    58801 gaaccggacg gccccagcaa cgcgaccatc tgaccagaag gaatatccag tgagatatcg
    58861 ttcagcacct gggtgcgacc aaaagacttc ttgatattgg caatctcaat gctcatgatg
    58921 ctcctcctgc tgcgcgcgtt tttcctgatt ctccaggcgc cactgcaaca tactctttaa
    58981 aaacagggtg ataatcgcca tcaacgtcaa cagcgcggcg gcggtaaagg agccgacggt
    59041 gttgtagtcc tgctccaaca attcaatctg taacggcagc gaaagtgttt cgccgcgaat
    59101 cgagccggaa accaccgaca ctgcaccaaa ctcgccaatc gcgcgggcgt tggtcagcac
    59161 cacgccgtaa agcagcgccc agcgaatatt cggcaatgtg acgcgacgga acatctgcca
    59221 gccggacgcg ccaagcaaaa tcgccgcttc gtcttcctgg ctgccctggc ttaacatcac
    59281 cggcaccagc tcgcgcacca caaacggaca cgtcacaaag atggtgacca gcaccattcc
    59341 gggccaggag aacataattt gcagattatg ttcatccagc cagccgccga gcgggccgtt
    59401 ggagccgtag aacagcagat acaccagacc ggcaaccacc ggcgatacag caaacggaat
    59461 gtccagtagc gtcagcagta actggcgtcc cgggaagtta aagcgcgtca ccagccaggc
    59521 cagcagaatg ccgaacacca ggtttaccgg tacggcaatc agcgcgatca tcaccgtcag
    59581 ccagatggcg tgcagcatgt ccggatcggc cagattctgt aaaaccggca tcagcccctt
    59641 gctgaatgcc tgcacgaaga tgtaaatcat cggcaccagc aggatgaacg ccgaaaccag
    59701 catcccgatg ccaatcagaa accatttgcc ccagttaatc gggcgcgcgt cataacgctt
    59761 caattgggta acttccgcca ttaatgacct accacacgcc gaccaaagcg actttgcaga
    59821 gtgttaattg agaacagcag cagcagagat gccgcgagga tcaccgaagc aatcgcgctc
    59881 gctgccgggt aatcaaactc ctgtaagcgc acaaaaatca tcagcgacgt cacttccgtc
    59941 ttccacgcga tatttccggc gataaaaatc accgcgccaa attcaccaag actacgggta
    60001 aacgacagcg ccacgcccgc caccagcgcc ggagaaagct ccggcagcac cactttgcag
    60061 aaactctgcc agcgcgttgc accaagcgtt tccgccgctt cttcatattc cgggcctaac
    60121 tcttccagca ccggctgcac ggtacgcacc acaaacggaa tgctggtaaa ggccatcgcc
    60181 accgcaatac caagccaggt ataggtgact ttgatatcaa acttcgccag ccattcaccg
    60241 taaaaaccgt ttacggaaaa gagcgaggcc agcgttaaac cggcgactgc cgttggcagc
    60301 gcaaagggta aatccatcag cgcatcaagc agcgtgcggc ccggaaagcg ataacgtgtt
    60361 aaaatccacg ccatcagcag accgaaaacg ccgttaaaaa tcgatgccac aaacgccgac
    60421 agcagcgtca ctttgtaggc cgcgaccacc tgcgggttgg tgatcacctc ccagtactgc
    60481 gcccagctca tctcagaaag ctgcatcacc agcgcagaga gcggcagcag caatatcagg
    60541 cacacaaaca gcagactggt gccaaggctt aaagtaaagc ccggcagcac gcgtcttgaa
    60601 gaaactgcaa acattactta cgccccgccg ctaacagctt gtctaactcg ccgccgctgg
    60661 tgaagtgggt tttcatcact tccggccatg agccaaattt gtcttccacg cggaacagtt
    60721 cggtctgcgg gaatttatct ttcagtttgt ccatgacttc cgggttattc acacggtagt
    60781 aatagtcggt gatgatggtc tgcgcctgtg ggctgtagag ccagttcagg taggcttttg
    60841 cggctttttc cgtaccgttg gcctgcacat ttttatcgac ccacgccacc gggaattccg
    60901 ccagaatatt ggttttcggg atcaccactt caaagccctg tgcttcatac tgtttacgga
    60961 tgttgttcac ttccgattca aagctaatca gcacatcgcc caagccacgc tcggcaaaag
    61021 tggtggttgc gccacgaccg ccggtatcga acacttcaac gtttttcagg aactgggtca
    61081 taaactgttc ggttttggct ttatcgccac cgtcagcttt atccgctgcg ccccatgccg
    61141 ccagataggt ataacgcgcg ttacccgacg ttttcgggtt cgggaaaatc agcttcacgt
    61201 cggagcgcac caggtcgttc cagtcgtgga tattcttcgg gttgccctta cgcaccagga
    61261 agcccatggt ggagtagaac ggcgagctat tattcggcag gcgcgactgc cagtcagccg
    61321 ggatcagctt gcctttgtcg tgcaggattt gtacgtcggt cacctggtta taagtgacaa
    61381 cgtctgcttt taagccctgc aaaatcgcca gcgcctgttt tgatgacccg gcatgagatt
    61441 gttttatcgt cagtttgtcg ccgccgttat cttttgccca ttgctgttca aacggcggat
    61501 tcagggcggc aaacagctcg cgggagacgt cataagaact gttcagcagt tccgttgcct
    61561 gtacatggcc cgccagcagc agagaagcga ccagcgcgag tgagttcttt ttcagtaagt
    61621 taacggccat tgcgcaccct tataaattta atgactttct aatagccatc atatttataa
    61681 cggacgtgaa aggagtaacg gttttatata ccgtttggtg atttggaagt taaaaaggga
    61741 ataagaactt ggttcaacag gcgtaattgt tgcaggcagt ttggacacgg acagcgcgga
    61801 gaaaccggag cgtacacata gtacgtgagg atttcgagca ctgcccgggg ccaaaatgac
    61861 aaatgaaata gcctggtgaa cttagttcaa gacgaaatcc tccccacaca tgcagggagg
    61921 aaacagaggt gaatcagata ccgacgctaa ccgtctccgg cagtgtgctg ccgccatcaa
    61981 tcacattctg tgtaccggtt aaatagctgg attcatccga tgcgaggaag gccgccagtt
    62041 cgccgacttc cagcggatcg gcgaggcgac acagcgggat tgcttttgcc atttcagtca
    62101 gcaccgattc tggatcttcc gggttcgact ggcgggcaat gctttccgcc attggcgtgc
    62161 ggacgtatcc cgggcagatg gcgttaacgc gaataccgga ttgcgcgtac tccaccgcca
    62221 gcgatttagt caggccaaca atcgccgctt tcgttaaggc atacgccgtt tcgccaggat
    62281 cggccaccat atcaccagtg actgaagaca tcatcacaat gcgaccatct ttgcgcgcaa
    62341 tcatctccgg cagcaccgcc ttcgtgacgt tccatacgcc tttaatattg atatcaatat
    62401 ggaaatcgcg atcttcatcg ctcatatcga ggaaactgcc cagacgacaa acgcctgcgt
    62461 tattcaccag gatatcaatg cgcccttctt tttccttcgc gcgtttgata gctgcggcta
    62521 ccgacgccgg gtcacgcaca tcggcgacaa ccgccgtaca gcgatgacca cgaccacaca
    62581 gttcgtccgc tagcttttcg atctcagggg agatatccag caagattagg ttcgcgccat
    62641 gacgtgcaaa agttctggca attccttcgc caattccctg caatgcgccc gtaatcagtg
    62701 ctgtcttgcc cgtgagttta cccattttaa tgctctcctt gttaatacag agcgttacac
    62761 tccattccct ctgaaaatac aggataaaca aaatattgct gcggatcagc tcactaattc
    62821 aggaaaatct caatccagtc gctgctttat gcatcatttc aggcgttgtg ttagctcact
    62881 gctgcgctga atcattttca atgacgccac atcattgatg ttgcacaaga ccaacaaaca
    62941 gtaaatcggt aacagaattt tgcgccgtgc gggtagacat cgacgagcta cgccattcgg
    63001 tttcaccgga aacggtatcg agcgtgaaat gcgccagccg ccgcagcgga gagtcagcca
    63061 gagaagtaat ggcaatcacc gttgccccct gctttcgtgc cgcttctgcg cacaagacaa
    63121 tctctttttt actgccgctg taagagatag cgatttgtac atcgcctttt ttcaacgcct
    63181 gggaaacggt ggcttgtacg tgcgtgtcgg cttcgcaggc cacgcgatag ccaattttca
    63241 tcagtttgaa cgacagatcg cgccccacca gcgccgatcc gcccaggccc gttatctgga
    63301 taaacggtgc tttactgatg acctcaatga ttttttgcag ccgcgcgtag tcgaacaacg
    63361 cacaggtttg ctccagcgcc agctcttttt cgcgattcag tttgcgggca atcacttcca
    63421 gcgaatcatc actggtaatc gaactgtgca ggtgcagcgc cgtggcattc gttttctccc
    63481 ggctggcact gtattcgcca attaacgcca tacgcaattc agtaaatcct tgcgcgccga
    63541 gcttctgggc aaatttcaca atgctcgact ggctgatacc gagctgtttc gccatctgcc
    63601 gtgaagagac ggatttcagc tcgctcactc tggcccgcag aaagtcagca attttttgct
    63661 cgttcccggt aaattcactt tccgcattgc gtattttcgt caggtacaac atcttccact
    63721 ctcctgggct ggcagcaaaa attccgctta agttcttatg acgctcttca cactctgcga
    63781 gtgtagactc aatgattcct ttaagtctgt caattcggga tatatgattc catacatctg
    63841 caattatttg atcgtaaata gtaaggtcac caccgatgca actagaaaag atgattactg
    63901 aaggctcgaa caccgcctcg gctgaaattg accgcgtatc gacgctggaa atgtgccgga
    63961 ttatcaacga tgaagataaa accgtaccgc ctgccgttga tcgcgtcctg ccggatatcg
    64021 ccgcggcgat cgatgttatc cacgcccagg tcagcggcgg cggtcgtctg atttatctcg
    64081 gtgcgggaac atccggtcgt ctggggattc tggatgccag cgaatgtccg cccacctacg
    64141 gcgtgaaacc gggtctggtg gttggtttga ttgctggagg cgaatatgcc attcagcacg
    64201 cggtggaagg agctgaagat agccgggaag gtggtgttaa cgatctgaaa aatattaatt
    64261 taacggcaca ggatgtggtg gttggcattg ctgccagcgg tcgcacgccg tatgtgattg
    64321 ccggactgga atacgcacgc cagctcggct gccgcacagt gggaatttcc tgtaatccgg
    64381 ggagcgccgt ttcaaccacc gctgagtttg ccattacacc gattgtaggt gccgaagttg
    64441 ttaccggttc ttcgcggatg aaagcaggta cagcgcagaa actggtgctc aatatgcttt
    64501 ccaccgggct gatgattaaa tccggcaaag tgttcggcaa cctgatggtc gatgtggtcg
    64561 ccaccaacga aaaactgcat gtgcgccagg tcaatatcgt taaaaacgcc accggatgta
    64621 atgccgaaca agcggaagcg gcgttaattg cctgcgagcg caactgtaaa acggccattg
    64681 tgatggtgct gaaaaatctc gatgccgcag aagctaaaaa acgcctggat caacacggcg
    64741 gctttattcg tcaggtttta gacaaggaat aacccatggc caaagagatc agcagtgaac
    64801 ttctgaacac cattcttacc cgtgtcggcg gaccgggaaa tatcgccagt tgtggtaact
    64861 gtatgacgcg cctgcgtctg ggtgtacatg acagttcact ggttgatccc aatatcaaaa
    64921 cgctggaagg cgtgaagggc gtcattttga ccagcgacca ggtgcaggtc gtttttgggc
    64981 ctggtaaagc gcatcgtgcc gcaaaagcga tgagcgagct gctgggcgat gcaccggtac
    65041 aggatgccgc agaaatcgcc gcccagaaca aacgtcagtt aaaagccaaa caaacctccg
    65101 gcgtgcaaca atttctcgcc aaattcgcca ccatcttcac gccgctgatc cccggtttta
    65161 ttgccgccgg tctgctgctg gggatagcga cgttaattgc cacggtgatg cacgttccgg
    65221 cagatgctca gggcacgcta cccgacgcgc tgaattttat gaaggtgttc agcaaaggtt
    65281 tgttcacctt cctagtgatt ctggtgggct ataacgccgc ccaggcattt ggcggcacgg
    65341 gcgtcaatgg cgcaattatt gccgcactct ttttgctcgg ttataacccc gctgcaacca
    65401 ccggttacta cgccggtttt cacgatttct ttggtctgcc catcgatccg cgcggcaata
    65461 ttatcggcgt gctgattgcc gcgtgggcct gcgcccgcat tgaaggcatg gtgcgccgct
    65521 ttatgccgga cgatctcgac atgctgctga cctcgttaat caccctgctg atcaccgcta
    65581 cgcttgcgta cctgatcatt atgccgctgg gtggctggtt attcgaaggt atgtcatggc
    65641 tgtttatgca cctgaacagt aatccgttcg gttgtgcggt tttagccggg ctgttcctga
    65701 tcgccgtggt atttggcgtg catcagggct ttattcctgt ttacctcgcg ttaatggaca
    65761 gccagggatt caacagctta tttcccatcc tttcaatggc aggcgcgggc caggtgggcg
    65821 cggcgctggc actctactgg cgggcgcaac cgcacagtgc gctacgcagt caggtacgcg
    65881 gggcgattat tcccggcctg ctgggcgttg gtgaaccgct gatttacggt gtcaccctgc
    65941 cccgcatgaa gccgtttgtt accgcctgtt taggcggcgc ggcgggcggt ttgtttatcg
    66001 gattgatagc ctggtggggt ctgccgatgg gcttaaacag cgcctttggg ccgtctggtc
    66061 tggtggcgct gccgctgatg acttccgcac aaggcatcct tccggcaatg gcggtttatg
    66121 ctggcgggat tctggtggca tgggtttgcg ggtttatttt caccacgctc tttggctgcc
    66181 gtaacgtcaa tctggactga tattatgaaa cggacaatgc tctatctttc tctgctggct
    66241 gttagttgta gcgtcagcgc cgcaaaatac cctgttctga cagaaggtag ttgatcaagt
    66301 atatgcggcg ttaaagcagt aataaaaagg ccagtcagta atgttactgc ctggccttta
    66361 atcaatgaat cagaaacgct tacagcgcca tcaacttgtc cagcgacggt gcgaaataat
    66421 agccgccggt taccggtttg gtgaaacgca acatcgcatc acgtttgcca tcggtatcgc
    66481 caaacatgct cagcagttgc tgctcaatgt tatgcagacg cgcgcagtag gcgcagaagt
    66541 acagaccgtg agtgccactg gcggtgccgt acggcaggct ctggcgaaca atcttcagcc
    66601 ctttgccatc ttctttcaga tcaacgcggg tgaggtgaga ggtttccgga cgttcgtcgc
    66661 cgtcaatctc ttcgttggcc tctttggtgc gcccgatcat catctcctga tcgtgaacgc
    66721 tcatccggtt gagctgcttc aggttgtgct cccaacgctg cacaaacaca tagctgccgc
    66781 cggaatccac gccgtctttg ataaccgcca cttcgcggcg cgtctcttca cccgccgggt
    66841 tttctgtacc gtcaacaaag ccgctcaggt cacgctcttc cacccaacgg aagccgtgga
    66901 tctcttcttt cacttcaatg cagtcaccaa aggcttccat cgccgcctga gcgacagaga
    66961 agtttacgtc gtgacgcaga gaaagaatgt ggatcaacac atcgaactgg gtcgtcggcg
    67021 caaggccttt accgtagccc ggaaaatctt tcagctcttc tgccccaacg ccgccgctca
    67081 gagcgcgcca ggtgttgtta ccaaaggcaa ccaccgcacc aagatgcgcg tccgggaatt
    67141 tcgcttcaaa agttgccagt ttgtcggcaa atgttttact ggccgcacgc agggcgtcaa
    67201 cttccccttt cacgttggct tcgatccaaa tcgccgcgcg gcaatgttct ggcaaaatgc
    67261 cactctgaac ctgagacatt gttcctcctg aaaataagaa tgccacgcaa tccgtggcat
    67321 tgttgagcgc tattttacgt gttttttcgc gacggcggtt tgttcagacg caaattaacg
    67381 ccgccagata attttactga ctttccagtt tttcagggta tcgtccgaag gcattaaccc
    67441 ttcaggacca cgccattccc cgctgaactg gtagctaata tgttgactgc cttcggcttt
    67501 acattcgacg gcacgattat catcaccatc agctttttgg caattgccaa atgctttgct
    67561 gtaaaggtcg ctaaacggtg taccgatttt aacaccggtg tcagcaggaa tatcgctatc
    67621 cagcacgtca atgcgactga tcgtgccttg atcgccgtta atcaccatcg ccacgttgtc
    67681 gcctttcatc acttcaaaaa agcgcaccac gttgccgttc gtggttttca ttccgctgcg
    67741 caggcgataa tcgccatcaa gcgcatcagc aatggcttgt tcttgcagtg gtgtggacgc
    67801 cgttaattca cccacgccct gctcgctcac tttggtggac gatccaaacc agttccacgg
    67861 gttagcggca gaccagttaa ctgacgacat cgtggaacag ccggtcagca tcaacggcat
    67921 agcgcataac attaaacgca gcgatttcat gtcacttcct ttggttattc aataacgttg
    67981 cttggagtgc aaattcacca aaaagtgccg ttattctttt tcttcattaa aacaagcgcg
    68041 taaacgtcga ttggtcagca gccagattaa cgccacaata tccgccacta ccagcgccag
    68101 accgataccg ctaacggatt caccgttcag ccacagccac ggttgccagc aaagtaaaac
    68161 cacctgcgcc aacagcaaca gaaaatagag cacatgccag gtgcgaggaa acgaagcccg
    68221 ccgaccgctc aacaaaaacg ccagcaccgc cggaataccg ggaatcagcc ccagccagaa
    68281 attatcgtga tcgggataaa acagatttag cagcgcagta ccctgctcac gcgacgcacc
    68341 ggcaatgaca aacagcaccc aggttcgcgc ctgaagcaat agcacaagcc agaagagcaa
    68401 gggtaaacgc aggcgtccgt gcgcatcata atggacagga tggaactcag tactcttcat
    68461 cttcaatcaa acgcttaccc agactcagca cgtcggcgtg ttcatatccc aggcgttcat
    68521 acattccgag caccatgtcg ttatcttccg gcacgttgat ctgaattttc gggcagccgc
    68581 gagcaatcag ctttttctcc agccggttaa gcaacgcatt ggcaattcca cgcccacgaa
    68641 actctggatg cacgccaaga taataagcag acccgcgatg cccgtcataa ccgcccatca
    68701 ccgttccgac cacttcaccg tttacctcag cgaccaaaaa caaactgacg tcatggttca
    68761 tcttacgctc gatgtccatt tccggatcgt tccacggacg cagcaagtcg caacgctccc
    68821 aaagggtgat gacctcttcg aaatcttcct ggcgaaatac gcgtatctcc atggtattcg
    68881 ttaccttttt gcgggttaaa aggctgatta tggcgtgaac ggtcgaatta gccaatatct
    68941 gacgaaaatc ggttgaaaaa gtggcataat ggggagttgt caactattga aatgaaaagt
    69001 aaaacaattc tcaacagcaa accgtcgtaa cggattacgc gatacgatat aacatctgga
    69061 actttattat tacaactcag gccgtatgag cacttttaaa ccactaaaaa cactcacttc
    69121 gcgccgccag gtgctgaaag ccggtttagc tgccctgacg ttgtcaggaa tgtcgcaagc
    69181 cattgccaaa gacgaacctt taaaaaccag caacggacac agcaagccga aagccaaaaa
    69241 atctggcggc aaacgtgtcg ttgttctcga tccaggccac ggcggaattg ataccggagc
    69301 gatcggacgc aacggttcga aagaaaaaca tgtggtgctg gcgattgcta aaaacgtccg
    69361 ttccattttg cgtaatcatg ggattgatgc gcgtttaacg cgttctggcg atacgtttat
    69421 cccactttac gatcgcgttg aaatcgccca taaacatggc gcagatctgt ttatgtcaat
    69481 tcatgccgat ggctttacca acccgaaagc tgccggtgct tcggtatttg ccctctctaa
    69541 ccgtggggca agtagcgcaa tggcgaaata cctgtctgaa cgcgaaaacc gcgccgatga
    69601 agttgccggt aaaaaggcga ctgacaagga tcacctattg cagcaagtac tgtttgatct
    69661 ggtgcaaacc gatactatta aaaacagcct gacgcttggc tcgcatattc tgaaaaagat
    69721 taagccggtg cataaactgc acagccgcaa caccgaacaa gccgcgtttg tggtgttgaa
    69781 atcgccgtcg gttccttcgg tgctggtgga aacctcgttt atcaccaacc cggaagaaga
    69841 gcggctgtta ggcacggcgg cgtttcgtca gaaaatcgcc acagcgattg ctgaaggcgt
    69901 gatcagttat ttccactggt tcgacaacca gaaagcacat tcgaaaaagc gataagttat
    69961 gaaacccgac gcacaccagg ttaaacagtt tctgctcaac cttcaggata cgatttgtca
    70021 gcagctgacc gccgtcgatg gcgcagaatt tgtcgaagat agttggcagc gcgaagctgg
    70081 cggcggcggg cgcagtcggg tgttgcgtaa tggtggtgtt ttcgaacagg cgggcgtcaa
    70141 cttttctcac gtccacggtg aggcgatgcc tgcttccgcc accgctcatc gcccggaact
    70201 tgccgggcgc agtttcgagg cgatgggcgt ttcactggta gtgcatccgc ataacccgta
    70261 tgttcccacc agccacgcga atgtgcgttt ttttattgcc gaaaaaccgg gtgcagagcc
    70321 cgtttggtgg tttggcggcg gcttcgattt aacccctttc tatggttttg aagaagacgc
    70381 cattcactgg caccgcaccg cccgtgacct gtgccagcca tttggtgaag acgtttatcc
    70441 ccgttacaaa aagtggtgcg acgattactt ctacctcaaa catcgcaacg aacagcgcgg
    70501 cattggcggg ctgttctttg atgatctgaa cacgccagat ttcgaccact gttttgcctt
    70561 tatgcaggcg gtaggcaaag gctacaccga cgcttattta ccaattgtag agcgacgtaa
    70621 agcgatggcc tacggcgagc gcgagcgcaa ttttcagctc taccgtcgcg gtcgttatgt
    70681 cgagttcaat ctggtctggg atcgcggcac gctgtttggc ctgcaaactg gcgggcgcac
    70741 cgagtcgatc ctgatgtcaa tgccgccact ggtacgctgg gagtatgatt atcagccaaa
    70801 agatggcagc ccagaagcgg cgttaagtga gtttattaag gtcagggatt gggtgtaact
    70861 ccctcacccc cattcccgca tccgctgatg caacgtcaac gacggcttct cggcaaacag
    70921 ctgctggtaa tccgtggcaa attgccccag atgccagaat ccccactgca tggcggcgtc
    70981 ttttaccgtt gtgctttgcg accacgggct tatcagttcg cggcgtacga cgttcaggcg
    71041 aatgcgtttc agccacgcgt ttgggccaat gcctaaaata gcgtgaaacg cgttttgtag
    71101 cgtgcggcga ctgacatgca gttgattaca caaatccagc accgtcaccg gttcggacat
    71161 gttttccagc acatattcac gggcgcggga aagcaaccga cggtaactct gatgactgat
    71221 gctttccgcc gtcaccattg gttgcgcttc ttccagcatc gcccccatcg ccattagcaa
    71281 attatccccc agcacttttc gcactgcagg ctgatggaga ttttccgggt tctcgcaaaa
    71341 cgtcgccagc gcctgttgga caaagcccca cagcgcggct ttatgctgct ctttcacttc
    71401 cagcgccgac tggctacgca gcatatgtaa tacccgatcc ggattgtgca aaaagttagc
    71461 ctgacaggtg atgacatctt cagacagcac tacgcccagg atcgtataat cgtccggcgt
    71521 acttaattca aactcggttc caccggggcg cgtcgctatt tctgcgcttc ccagacattg
    71581 cgaaccgata aatccctgct caccgcgcgt cgccggaatg ccaaaccaga acgagttcgg
    71641 ccagaccagg cacgactgac gcagcgccag accggtgtat tcacgaaaaa cctgaatatc
    71701 atcgagtaga atttccgtaa attcaccatg aaacttgccc ggatgcagct gatcgtaaat
    71761 ctgctgctag gcggtaatcg tcaaagcatg ttcatagaca tccgttgtct gtcgttgatg
    71821 aacattatcc acttcgacct tcggcgtgag cttcaggttt tcgggtaagg gttcatgata
    71881 aagatggtgc aaattggctg tacgggtctt tttcatgatg ttaatgccgg gtgttgtagg
    71941 acacccggca cctccgacag gttaatgggg cttgagacga taacgactac tgcgtttacg
    72001 taacgtcccg gcagaaaaga gctgttcgag cgcatttctg gctttttcca gcggccagcc
    72061 aaagtgggcg gccacttctc ctgccgtcat tccctgacat actgatgtta acagcgccaa
    72121 caactcgtca gcagattccg cgacgataac tggcgcgtcg ggttccttta cgggttgctt
    72181 cggctggcgg ttaaagccag taaccagcca ctgggtgtca tcgtctggcc gaccgatctc
    72241 cttacggctg atgacacgcc cggtacgcat cgcggcagca caacctgcgt ccagcgccgc
    72301 acgacacgcc gccagatcgc cttccaccac cagcgttaag cgaccagggt caagcacttc
    72361 gtgactgagc agacgaacgt tagctgcttt gagcatggca tccgctgcat ctatcgcggc
    72421 gaccatgccg tccacttcca gcaatcccag tgcattgatc atcggcaacc tccgttacgc
    72481 acgctggatt ggattacgcg cgatttccag cactgcatcg gtaaaggcgt tacaggctgc
    72541 tttacacgcg gcctggctac cggttaaaaa tgctgccgag tagttggttt cagacggtgg
    72601 cgggacgtag gtcaccagct gtacgtcggc agatttcaac gcggcatcaa tgccgtaggt
    72661 cgcttccagc ggcggtgcca ccagatacgc catcggatcg ccaagcgtga tcccggcggt
    72721 tgatgaaaga taagaaccgg tacgcgaaac cacatgcgcc aggaaggcgg tgttttctgc
    72781 gtcattggcc cactggaaag ctgcgccatt ttcaatatgt gcaaccatcg catccagacc
    72841 ggcacgcact tccgccgggt ttggcccacc aagcataatc agcacttcac cggcagtcgg
    72901 tgacgggccg tgtgccgctc ccgcatacag cgagcgacca tataccactt cgaccatcgc
    72961 ttgtttggtc gcttcgtcag ccgcaatgta ggttacgtcg tcagaatcgg cagaaatcag
    73021 cccgaggcta cgaatatgcg gcggcaattt tagttcacgc gcaaattcag cgttaacgga
    73081 ggcaatcacc cgcatggcgg ttaccgacgg tcgaatcaaa tctaaagctg gcatgatgcc
    73141 tccttatcgg gtcatgttga tgccggatgc tttctgctcc agcatacgtt tcgccaaatc
    73201 cacaatgacg gcacagcagc acgaaagaga agcaaaagtc agctacctgg tacactaggc
    73261 agcgggtcag caaagggggg caaatgcccc ttttttcatg tcagataatc agatcattac
    73321 ctcaataaac taataatcac atcgcggtta caaccagttt gatagtgata gctcaaaccc
    73381 agacgtggcg ctggctgcaa gcaaaataag ccgcaaaaat gcgaccccat gtttactagc
    73441 cgcaaaaatg cggtcctatt tgccagaacc ttttattatt caattggtta gtgcaagaaa
    73501 aagcgtgatt gctgttcaag aagtatccag aaaggtcatg attttgacgc tgaaaaaaaa
    73561 taggtgggca tttttgccca ctcactagcc gcaaatttgc ccactcacta gccgcatttt
    73621 tgcggcccaa agtaataaat acagagaaat aataaataca gatcagcgcc aagatttttc
    73681 gtcttttcgg cggaatcatg agactagtgc ttacgtcctg ataacctcca gagcagacct
    73741 taacttctgt gcagcaggac tacgctcatt gccgctcggc taggtcaaat cctcacgata
    73801 aagctgttcg gattgtgacc ataatgttaa aggactcttt aaatcattcc ttgacgaatt
    73861 aactagctgg cttaagcgga atagcatgca ggataaaaac aaaaccagct tagcagtggg
    73921 gagagcttcg cttcgctcgt ctcctggggc caatgctcaa tgaaacgacg taaggcacgg
    73981 agcgctcacc ataggtagac acataaacat ctgagagcgc accttctatc gcgctacgcg
    74041 ctcaataaaa accaacgaca ccgccacact gcaagaccaa tggtttttat cgcgtataca
    74101 gaccagacag gaaacagcag caaagcagcg tatagccagg tgcagacagc acactcacac
    74161 gacgcacagc aggcttaagt gatcactcag gaaactttat ccccgccgcc gatcaccgcg
    74221 aactatcatg accgcgccaa attcgaaagg aatggcccat agaacgcgat gaacacacat
    74281 aagcgctaca cttacttaac gtcgccacag agcgattgag agcgcttaaa atcgatgcga
    74341 tttcattaaa tttttctcac tcgcctgaat ggtggcgatc aggactgatc gcaacattgc
    74401 cccgcctcga tattgagcgc ataatagata catacattaa gcggaaaaga gataagttta
    74461 ttgtgaaaat cagggggtta catatcatgc gcatatcaga acaacaacgg cagatcctcg
    74521 actatctggc aaaaaacgaa tcaatcaccg cacgacaagc cgcagatctc gtttatggtg
    74581 gcaatgttac ccgcgtccag gttgacgccg cccgccgctc actcatgacg atggttagta
    74641 atgggctaat caggaaacaa ggccgcgctt tcgtagctga aagacatatc aatcacgctt
    74701 atagccagga cgttgaagaa ttgcgccgcc agatcctttg ggtattcagc aaacagggtc
    74761 aggagtattt cctttctgaa cacccaaatt tcgccgccaa agagttaaca gtaagcggat
    74821 tgtcgtgcgc actggattat cttgtcggtg aaggttgcgt tacttacaag cagctatggc
    74881 acgccctgaa tacgcttgta gaagatggtt tactcattgc cacgcgtgag aacatcgaat
    74941 actggataat gccgcgttca ggtcgccgca atcatgcacg gtatatttca ggcaacagat
    75001 acagaacgcc ggactttaat ccgacacagg aagatcggga ccgtgccgaa atgatgtgca
    75061 gggaacagga gaaaagaatt atggataagt tattttcgat gcagtgaaca caagcgggcg
    75121 caatgcccgt ttttattgcc ttattccacg cctgacgaat caatttgatc acacctgaga
    75181 aaatgcatag ccagaagtta aggccgttat attgcgattg ggagcgtttt aaacggtatc
    75241 aatttaatta attctgatgt ggtgatttgc aggcaaaaaa aacccgccgg agaagcggga
    75301 aatagagaca tatcgttgta gttattaaca gatcactttg aggtcgaggg cggctaaccc
    75361 tcgctaatat tatcccgcta ttaccttgcg ggtgcaaata atcttttatt gctttgatga
    75421 tttaataaat agcacttttt gctaacaaaa accgctgtca atatatggcc aaaaatgtca
    75481 acagaaaaaa taaggtttat aatattaagt atcacatggg taatgggttc agggaatcgg
    75541 aatccaccgc ctcacggaaa ttcaaaattt gcttcttctt tcctccgtgg gcggcttctt
    75601 tttcgtatcg aggtatttat gaaagcaata acattagaac agttcgcagc aatggcgaat
    75661 gtatcgacca gaacgctgag gcgctggcta aaaaaaccat caaccggagt ccctttgcca
    75721 tttaccccac gcggtactcg ccaattattc tgggagcacg aatgcaaaga atggttgtac
    75781 agaacagaac gataataatc acgcatcaca ttttgaggta aacatcatga ccaatatcga
    75841 aagattagaa cgctgtgccg atattatgcg acgcagatgg atttatgacc ctgaagcggg
    75901 tttattgttt agccgtgaaa caaaagacgt tatcaaaggc agcatgacca ataaagggca
    75961 tttggttgta acggtacatg aaggtaaatt caaagtgaca ctcacctatc agaaagcctg
    76021 ctatgtatac acatacggcg cttatgacga gacattatat gaagtgcacc acgttaattt
    76081 gaacaaacag gataaccgca tcaagaatat tcgcctgatg ccgaaagaaa agcaccgccg
    76141 gatacataaa aacgtccgca aacttattcg gctgggccac acacaatggc ttcaaccgca
    76201 attaattgaa caatcactat aaacacagga gtaaatacca tgcattcatt agacagttat
    76261 tttcagcgca cgacagcccc taaatcagcc gcccaggaac gccgtgagga attccatgag
    76321 aaagtgatgc gttctgctga ttatattgcg gacaagttcg tggaaacggt tcgtccactt
    76381 gttgatgaag tagctgataa acttcagtct gaaatgccgg aagatatgga aggcactgca
    76441 aagcggcgac tcatttgcga attaagtcgt cgttttggcg tgtcaataag cgcttttaaa
    76501 taatcgaaaa aaggggggaa agataatgga cgcatttgag gtactggcaa ataaaggcgc
    76561 tgaactggta gcacagcgtg ataaaaccgc caacgaaggg gaacgcgcac tgctaaataa
    76621 acagattaaa gcgatccgta tggcccagtt taaattaatc agtaatgaag ttatcgaaac
    76681 aattaaaccg cagatcccac aaatcgttgc tgacgctatt aaagccgccg gactggagaa
    76741 acgcattgcc gggctaaaaa cgaacgcaga gaaaatggag atgtacaaac agagcggtgc
    76801 tccgaatccc gatgagtact tctcaccgga tgttgagttt atggcgcagg ttgaggaacg
    76861 tctgggtagc tgtctcaccg aggaacagcg tcgctatttt gatggagtgg atagttcggc
    76921 aggtattgac ctgaactcgt atttcggtcg tgaaattgag cattttgatg ccgccccgtc
    76981 tatgccggag ccagaaccag aaatggccac cgatcctgaa gctgagcaac ggaaacgcgt
    77041 attcaatgcg atagggattc ggtactgatc ggataaaaga aagccgggaa ttaccccggc
    77101 tgacttaaca atgtgaccaa tgaacaaatc ggacaagaca aacaggataa gcagtctcga
    77161 aacaatgttc gggaactata ttagcacaag tccggagaaa atcatcatgt tggcactacg
    77221 catcatgcag ggcatcgcta agaccctcgc agaacatgtc ttagacctga aacattcgcc
    77281 actgagcaaa caggccatga agcgccaaac gcttcgtctg tgggctgaat attcgcttgg
    77341 aactatcaat aaaatcatcg acatgaaatc ggggccgagc aaccagagcg cggaggaaat
    77401 ggagttcatc aggcgtttaa ttctcattcg cagagacatt catagccagt tacacagcgt
    77461 gggaatcgat attaatgatg ggacgggcga ttaattttcg gacaagtact atgcgcgcgc
    77521 gaggagctga acatgaagca agatattgca gacaggctgg agatactcga aggccagcgg
    77581 gcagaagcga aacaactacg taagcaggcc agaagagcgc acaggaacaa cgaagcagag
    77641 ttactaacca aatacatcag tttcaccaat tactgcatct acgagtgtta caaggaagat
    77701 gcagaggact ggctggattc attgccagaa cagtactgag cagaataaca ccacgtcatg
    77761 ttgttctgag gtgagcatat cacctctgtt caggtggcca aatttagtgt gccacttcag
    77821 catcaccgtt gaaatcacaa taacgatttt tttgtaaata tgctctcaca tatgtaggaa
    77881 tagaccaggt tggcattgaa acacatctac catgaccata ataaagtaat tttttgatat
    77941 gtttatggtt ttatattaat taaaatatat ctaaatttga tagttttaaa attgcaaaat
    78001 ccagattgtg tttgtaatgt ttaatctgat tgtattgagt ggcgtttatt atgaaaaata
    78061 aatcaggtaa ttcttgttat agagaagcag tcgatattca tagctcactt gagctttcat
    78121 ttatattgca taaatatcat ttgcgtgtag atgagaagaa atctcgaaat gtattcagcg
    78181 ccatacgtga tgagctcatc cttgatgggg attcacgaca aaacttagct actttctgtc
    78241 aaacatggct tgatgatgaa atccgtgagt tgcttaattt agctattgat aaaaacatga
    78301 tttataaaga tgaatatcca caaatggcgg aacctgaagg tcgctgtgtg cgtatgcttt
    78361 ccgattcgtg gagtttcccc gattcgcgac acaccttagg ttgctcaacc attggttcat
    78421 ctgaagctgc tatgctcggc cgcttggcgt taaaatggca gtggtgtaaa aaacgcgaag
    78481 tacaaggtaa atcaaccgaa cctgatttgc gatcctgtgc aaatatgctg gcataaattc
    78541 tgccgttact gtgatgtgaa attgtgtgaa attccacttg atgatcatca cctgataatg
    78601 aatgctaaag aggttttgaa gcatgtcgat gaaaacacta ttggtgtcgt tacgacttta
    78661 ggtattactt atttttgtaa agcagattgc gttagtgttt ttaagataag tgttaaagtt
    78721 aatattagtt tttatattta catcgtaatt atatgtttct attttaaaaa tgttcgagag
    78781 tataaaaatg tctaatgcta atatcgcacc caaaagtaag aaaatgacag tggggacact
    78841 cgctatcatg aatataactg ctgtagtaag tctacgattt ataccttctg aagcagaata
    78901 cggcttaggt gccatttttt actatacctt tgctgcaatt atgtttttag tacctgttgc
    78961 ctttgttgcg gctgagctag ctacaacata tccacaaaaa gggggggcat ttcgatggat
    79021 aggagaagca tttggggctc gctggggttt tgtaggaatg cttatgacct ggctacaagt
    79081 catcccctac tttccaacag tactgacatt tggtgcagtc tcggttgcat ttattgatgt
    79141 tgatatgggg gtggcagaat caattgccag taataaatgg tatattttat ttttcgtact
    79201 atttgtttac tggggagcta tttgtgttgc attacgtggc gttggtattt tctcgcaggt
    79261 atctaagtgg tgcggtatta tagggacaat tattccagct attatccttg ttattcttgg
    79321 tttctcctat ctgtggttca gcggtaaacc agcggtgatc ccattaggtt ggggtgatct
    79381 tttccctaat atagctaact tccagaatat tgttcttgcc gccagcatat ttcttgctta
    79441 tgcagggatg gaaatgaatg cggtccatgt taacgattta gataatccaa ctaaaaaata
    79501 cccaattgca attaccatcg cttctatagg tactattgca atattcctgc tcagtacttt
    79561 aggggtcgcc tttattattc ctacagataa aattagtcta actcaaagtt tgttggttgc
    79621 ctacgatatg ctgttcgaat gggctggagt gccatggctt ggctctgtga tggcatttat
    79681 gctagcaatt ggcgtactgg gtggggtggt tacttggatt gcagggccta atacaggtgt
    79741 attagcgatc gcgaaagctg gttatttacc taagtttttc caaaaaacca atcgtcatgg
    79801 aatggggcac catctaatgt ttgtacaagg tataattgtc tcagttcttt ctgttacttt
    79861 tgtgataatg ccttcagttc aagcagcttt tcagatttta tcacagttaa cagttatgct
    79921 ctatttagtt atgtatatgc tcatgttcgc tagtgctatc tatctacgac attctcaacc
    79981 tgatacaaaa cgcccatatc gagcccctgc tttatttttc tgggcaacac ttggattctt
    80041 agggtctttt ctggcttttg tattaagttt tattccgccc gagcaaatcc caactggtag
    80101 tccaaccagc tatgttcttt atttgttagt attagttgcc ctatttttat taattccaat
    80161 attaatttac gccttccgaa aatctgactg gaaagatcca aactctgatt tcgaaccatt
    80221 ttcttgggaa aaaagaacta gtaattaaat tttccataag ctacaaaaga tgaggttaaa
    80281 tatgtttagt gctgaatctc caattggtgt aatagactca ggtgtcggtg gattgacatc
    80341 ggtaaaggaa ataattaatt tgcttcctgg tgaagatatt atctattgtg gagataatat
    80401 gaatgcacct tatggcaacc gttctgccga tgatatcatc agtttgacta aaaaaatgct
    80461 gactttttta cagtcaagga atgtcaaact agttgctgtc gcatgtaata ctatatcctc
    80521 aactttggaa tccgaagaat acagcggata tgctaagagc tttccgtttc ctatcttatc
    80581 catcattgaa cctgcagtag aagatgtgat tcgccagcaa tataaaaatg ttggtattat
    80641 cgctaccgag tttactatta aaacggggtg tcacaaagag ctaataaaaa aattgaattc
    80701 aacaataaat gtatttggag aaccaagcaa aaatcttgca atgttaattg aggaaggtaa
    80761 tcttaatgct cctgcaatct tgaatgacat caaaaaacat gttaaccatt taatttccct
    80821 ccatccagta aatgaaatta ttctcggctg tactcactat ccaattgttc agaatttttt
    80881 tgaagcagtt gcaccggata taaaatttat taatcctgca catgatcagg ctatctctat
    80941 taaaaatcac ttgggacagc taaatttatt aaactcctca aatataggaa ctttgcaaat
    81001 aaatactagt ggaagtatgg agatctacaa aacggtacta ggggaattga gcattacgaa
    81061 acctcataca ttcagtatca ggcagtttta aatttatccg gtacaccgta aaatcccgtc
    81121 cttctgaact gcaccccaaa agttggacac caactgagta aggtgcattt tatggacaaa
    81181 cccagttaca tactgggtag tgcatccaat tagtagaaca tgtgtttttc gataaacgct
    81241 ccgatcactt tttcgtggat ctcaactgag cgtgagaagc agattgtttt acgacccaac
    81301 cgcttaatgc gggtgcgtag cgtcagatta ttacgctcaa tgcgttgggt gaatattttg
    81361 ccggtcagat gcttattctt cggcacctcc cggccatagc tgccccagtc atcgctggtc
    81421 agcatgccga tgttgaaggg tgtaagcagt gccagtagct cccggcacgt ttgatcggtt
    81481 cggggaccaa aagtgtaggc cagtacaccg cctgttttgg tgttgtacgc gtacccgagc
    81541 caatgttgcc gggctttact gccaacgtag ctccattgct catcaagctc gcagataaac
    81601 gccacatcag catgggcaac aggcgaagaa gttattcgct ttggcgcgag ttttttaaag
    81661 tccggatgac ggtgttacta ccaattttca gtgtcctggc ggtatcgcga accccggcac
    81721 cattaaaggc catttcagtt atcagctctt tcatacccgg cttacgtgct tgataagtat
    81781 aagtgaactg aaacacacgg tggcagtcac ggcagcgaag tctgtcacgg cctttagggt
    81841 tctgaccatg gcggtaaacc tgagctgact gacaacgggg acaatgaata ttaacgctgg
    81901 tcatgagata acctcaaaag cccgtattat acatcagatt caactaatta gaggcatcac
    81961 ccatccagca ttataaaaca tactaacaat aactaaatta taataatcac aacttacaat
    82021 caaataataa atgcataaga ttaacaatga ttttttcggg ttatatttat ctaaaaaagc
    82081 agaatattat gtaacttatt attttctaag cgaaaattta ttgtgcggtc agactaacaa
    82141 acttgcgtac cacccgaaga acatgcgaag atttaagtat tctttctttt ttggttttta
    82201 tttttcattt tctcttttct tctctttccg gttctgttct ggttttggtt ttttcctttt
    82261 tatttttatt ctttcttcag ggttatgcta aaacaccaaa acatttttca ccacaaaagc
    82321 atccaatcat cttgccagac caatcacctt aaccggatac actgccggat ataatcccac
    82381 taacacccag acaccaagca ccatacctga taactaccgt attcaggaag gaaatgcgag
    82441 tgatttcacc gtcaccggca caaccgatcc gccaaaaaga ggcgtaccaa tgtcgatata
    82501 gtcccccgcg cggacaatca cttcgtcaat gactgccaac gggagttgtt gtagctgtgg
    82561 gcgcaacaac atgcccagag ctttaccaaa gtcctgcccg gccaccacca gcaggggatg
    82621 cggattcgga aaacgcgcga cgaaatcgac cagcgcgttg atgaccgtca gaaccgcggc
    82681 gtaacgcaca ggcagcgagg cgggaagcgc cagcacgtac acgtcagttt tgggatcaag
    82741 atccagctga agcagcgcct gttgccaggc actcaccaga tccgtttcat caatcgggat
    82801 cgccaccggc aaattgcgca gcggcagttg tacgccctcc agccagattg tgctgccaga
    82861 gagcgaaagg gtatgtgcac ccgcgccaat caccgtggag cgtacggttt gcgccggaaa
    82921 ctgcacattc atctcacgca ggcgcggatg gtcatgcagc gccgttgcca gaagtgggcc
    82981 aatatcggca aaacagaacg ggttggcggg ctggtggcga taacattcgc ccacgcctcc
    83041 agaaagcgta atgatttcgg gcgtaacacc tgcgggcagc aaaccggttt gcatcaatgc
    83101 ctgcgcgagc ggcgaaagcg ttccgtcaat cacttcgaca atcagcgccg ccatccgccg
    83161 ggtaacctgc accagctgcg cgccggtcag cgaacgggcg tcagtgcctg caccgaagca
    83221 ctcatccaca atcatctgcc ccggtttatg agcgtaaacc acgcgcccct ggctgtcggt
    83281 ttccagcagg cgaccaccga cgttgagaca ggcagtgccg ctgatttttc cggcatcgaa
    83341 cagggcgtag ttcgcggtgc caccgccgat atcgatattc agtacccgac acagccgttg
    83401 ttcagaaagg gtttgcgccc cggctccgtg accggcgatc acggattcga ggtgcggccc
    83461 ggcgctggcg acgacaaaat cgcccagcga ctgtttttcc ttcatcactt tttccatcag
    83521 ctgtgcgacc aggctttcgg taaactggcc ttccggcagc tgggcaatga tggtttcgcg
    83581 gatgcgctgg ctttcggttt tgtcggctgg cacgctggcg ggcacggacg ccgttaccgg
    83641 gatcgattca tcacattcgg tgatggtaaa acccagtaga tccgccactt cacgggcttc
    83701 cggggtaata atgctggcgc gcagaaccac ggacattgcc tgttcgccgc gcgcgtgtgc
    83761 ttcacgaata tcattcgctg tgatgagttt tttcacctgc ttcctcctgt ttggtgagag
    83821 atgccagata atccaccagt tgctgcacgc tttgcgggtc gtggctgttt agctcgaaaa
    83881 tcggctcttc gaaacccgtc tcaagcaata actttcgcgt tgcggcgacg tcagcatctg
    83941 gcatgtccgt tttactgatg acggcgattt gccgtttgct gacgccaata tccagcaacc
    84001 cggcaggcaa ccgactttcc gggtcatttg cgccatggac atagatcagc atgtctacat
    84061 cctgtagcgt ggtaattaag gcgtgatacc agcggggatg gctaaaatat tcacccggcg
    84121 tatcaatatc gcccttatca ttaaattcca cggccagtgt ttttctggcg agggtataat
    84181 tcccctgcaa cgcattaaat aacgttgttt ttcctgcccc gacgctaccc acaaacgcaa
    84241 ttcgtttcat gaatgcctcg tattaacttt tggtcatttc acaaagcgta taatttaata
    84301 agcgacctaa accgctgacg gtttgtgata acgcttcttc tactgcgccc accgagccat
    84361 aaatcaccaa tgcaccgctg aagcggtcga gaaaaccgat atgcacatca gcggctttca
    84421 gcgctaaatc accggcgatc atcgccgttt cgccgggagt tagcgtcata atgccaattg
    84481 cgcccgcatc cggaacgccg attttcttcg ccagttcctc accgggatgc gcgatgagat
    84541 gcgccagtgt gacctgtttg ccgggcacaa attcctgaat gatgcgttct ttatccatga
    84601 gtcgccgcct gtgaaaaact ttcgtgtatc gtgacaattt ttcgtcagag taaataacaa
    84661 aattcggcaa gcgcgtttaa aaggtgaggt agatcaataa gaatggagag atatttactg
    84721 ttatgaataa actttcatat gcgacgcatg aatataaata aaataatatc gagatatttt
    84781 atatactgat ttttttatta ttcgcaaact cagtaataag ctttgctaat atatttcagg
    84841 aaaaccgctt accctgaata ttcagggtaa gcgtgagagt taagaatgaa ttacagcggt
    84901 tgggtttgcg cttcaaccac ggccagcgcc accatattga cgatacgacg caccgatgcg
    84961 atcggcgtta acacgtgaac cggtttcgcc acgcccatca gcaccggacc gacagtcaca
    85021 ccttccgagc tggaaacgcg cagtaagttg taactaatgc gggcagcttc catgttcggc
    85081 atcaccagaa tattggcaga acctttcaaa gggctgtccg gcatacggtc gttgcgaatc
    85141 gcttccacca gcgctgcatc gccgtgcatt tcaccatcaa tcatcagatc tggcgcacga
    85201 tccctgacca gttccagcgc ctgacgcatt ttgctcgacg acgggcagtc agaagaacca
    85261 aagttggagt gcgacaacag agcaacgcgc ggctcaatac caaaacgacg gacagtttct
    85321 gccgccatca aggtgatctc cgccagctct tctgcgtctg gttcgtcatt gacgtaggta
    85381 tcggcaataa aggtgttacc actcggcagc agcagcgcat tcattgcacc tgcggtgtga
    85441 acgccatcgc gataaccaaa gacatttttc accacactaa aatgttcatg ataatcgccc
    85501 accgtaccgc agatcattgc atcggcttcc ccacgctgaa ccatgatcgc gccgatcact
    85561 gtcgggttac tgatcagcgc acgctgcgcc tgttcctgag tgacaccgcg acgcttcatg
    85621 atctggaagt attcggtcca gtactcttta aagcgcggat cggattcgtt attgacgatt
    85681 tcaaaatcaa cgcccgcttt gatctgcaag cccagtttct gaatgcgcat ttcgatcacg
    85741 ttcggacgac cgataaggat cggtttcgcc agtcccagcg ttaccagttc ctgagtcgca
    85801 tgcagaacgc gcgcctcttc cccttccggc agaactacgc gcttcggcgc tttgcgagcc
    85861 tgggagaaaa tcggcttcat aaacaggttg gttttgtaaa cgaactcagt cagcttgtcg
    85921 atgtagacgt cgaaatcagc aatcggacgc gtcgccacgc ccgactccat cgccgctttt
    85981 gcgaccgcag gagcgatctt aacgatcaag cgcggatcaa acggttttgg aatgatgtat
    86041 tccggaccaa agctcagatc ctgaacgcca tacgctgaag ccaccacttc actctgttct
    86101 gcatgggcca gttctgcaat cgcgcgtacc gccgccagtt tcatctcttc gttgatggcg
    86161 gttgcgccaa cgtccagcgc gccacggaag atgaacggga agcaaagaac gttgttcacc
    86221 tggttcgggt agtcggaacg gccggtacag atgatggcat ccggacgcac ttctttcgcc
    86281 agcggcggca gaatttccgg ttccgggttc gccagcgcca ggatcattgg tgcccgagcc
    86341 attttcttca ccatttcctg ggtcagcact ttcgggccgg aacagcccag gaaaatatcc
    86401 gcaccttcaa tcacatcatc gagggtacgt ttgccgtcat ccaccaccgc atatgcggct
    86461 ttggtttccg ccatatttgg ctcacggccc tgatagataa cgccttttga atcgcaaacc
    86521 acgatgttat gtttttgcag ccccagcgct accagcaggt tcatacaggc gattgctgcg
    86581 gcacccgcgc cggaaaccac catccgcacg tcggagatgt ttttctccac cacgcgcaag
    86641 ccgttgagga tggcggcagt gctgatgatt gccgtgccgt gctgatcgtc gtggaatacc
    86701 ggaatattca tccgctcgcg cagtttctgt tcaatataga aacattctgg cgctttaatg
    86761 tcttcgaggt tgatgccgcc gaaggtcggt tcgagcgcgg cgacgacttc aataaatttg
    86821 tccgggtcga gttcatcaac ttcaatgtca aatacatcaa tcccagcgaa tttcttaaat
    86881 agaacgccct tgccttccat caccggtttg cctgccagcg cgccaatgtt gcctaacccc
    86941 agcaccgccg taccgttaga gatcaccgca accaggttac cgcgcgcggt atatttgtag
    87001 gcttttagcg ggtctttttc gatttcaaga caaggtgcgg caacgcctgg tgagtaggcc
    87061 agcgccagat cgcgctgcgt tgccagaggc ttggttggag aaacctggat tttccctgga
    87121 actggaaatt catggaaatc aagtgcactt tgttttaact ggtcatccat ttggttgttc
    87181 ctttcacgta acgttcacaa ataaagtgtg tgggcaacag cccctgccca caacgtggcg
    87241 cacattatta ccctgccgga gtctacagac tttgagcaag tccaaactct caccattaat
    87301 ataatgtttt ggtaataatc ctataacact gatgttacct gcttaatcca gcaataccat
    87361 gcctgtctgc tatgcttttt tgatgcgttt agcgaaattt ctcagaagtg tgaattaacg
    87421 cactcatcta acactttact tttcaaggag tatttcctat gaacgagtta gacggcatca
    87481 aacagttcac cactgtcgtg gcagacagct gcgatattga gtccattcgc cattatcatc
    87541 cccaggatgc caccactaat ccttcgctgt tactcaaggc tgccggatta tcacaatatg
    87601 agcatttaat agacgatgct atcgcctggg gtaaaaaaaa tggcaagacc caggaacaac
    87661 aggtggtcgc agcgtgtgac aaactggcgg tcaatttcgg tgctgaaatt ctcaaaatcg
    87721 tacccggttg cgtgtcaaca gaagttgatg cacgcctctc ttttgataaa gaaaagagta
    87781 ttgagaaggc gcgccatctg gtggacttgt atcagcaaca aggcgttgag aaatcacgca
    87841 ttttgatcaa actggcttcg acctgggaag gaattcgcgc ggccgaagag ctggaaaaag
    87901 aaggtattaa ctgcaacctg acgctgctgt tttcttttac ccaggcgcgg gcctgtgcgg
    87961 aagcaggcgt ttttctgatt tcgccgtttg tagggcgtat ttatgactgg tatcaggcac
    88021 gcaagccgat ggacccgtat gtggtggaag aagatccggg cgttaaatcg gtgcgcaata
    88081 tctacgacta ctataagcaa caccactatg aaaccattgt gatgggcgcg agcttccgtc
    88141 gcaccgaaca aatcctcgcc ttaaccggct gcgatcgact gactatcgca ccgaatttgc
    88201 tgaaggagct gcaggaaaaa gtttctccag tggtacgtaa attaatcccg ccttctcaga
    88261 cgttcccacg cccagcaccc atgagcgaag cggagttccg ttgggagcac aatcaggatg
    88321 cgatggcggt agaaaaactg tctgaaggca ttcgtctgtt cgctgttgat caacgcaaac
    88381 tggaagatct tcttgccgcc aaactataaa ccagccacgg agtgttatat gtcccgaaaa
    88441 gaccttgcca atgcgattcg cgcactcagt atggatgctg tacaaaaagc caattctggt
    88501 catcccggcg cgccgatggg catggctgat attgccgaag tgctgtggaa cgattttctt
    88561 aaacataatc ctaccgaccc aacctggtat gatcgcgacc gctttattct ttccaacggt
    88621 cacgcgtcga tgctgctcta cagtttgcta catctgaccg gttacgacct gccgctggaa
    88681 gaactgaaga acttccgtca gttgcattcg aaaaccccag gccacccgga gattggctat
    88741 acgccaggcg ttgaaaccac caccggcccg cttggacaag gtttggcgaa cgccgtcggg
    88801 ctggcgatag cagagcgtac actggcggcg cagtttaacc agccagacca tgagatcgtc
    88861 gatcacttca cctatgtgtt tatgggcgac ggctgcctga tggaaggtat ttcccacgaa
    88921 gtctgttcgc tggcaggcac gctgggacta ggcaagctga ttggttttta cgatcacaac
    88981 ggtatttcca tcgacggtga aacagaaggc tggtttaccg acgatacggc aaaacgtttt
    89041 gaagcctatc actggcatgt gatccatgaa atcgacggtc acgatccgca ggcggtgaag
    89101 gaagcgatcc ttgaagcgca aagcgtgaaa gataagccgt cgctgattat ctgccgtacg
    89161 gtgattggct ttggttcgcc gaataaagca ggtaaggaag aggcgcacgg cgcaccactg
    89221 ggggaagaag aagtggcgct ggcacggcaa aaactgggct ggcaccatcc gccatttgag
    89281 atccctaaag agatttatca cgcctgggat gcccgtgaaa aaggcgaaaa agcgcagcag
    89341 agctggaatg agaagtttgc cgcctataaa aaggctcatc cgcaactggc agaagagttt
    89401 ccccgacgga tgagcggtgg tttaccgaag gactgggaga aaacgactca gaaatatatc
    89461 aatgagttac aggcaaatcc ggcgaaaatc gctacccgta aggcttcgca aaatacgctt
    89521 aacgcttacg ggccgatgct gcctgagttg ctcggcggtt cggcggatct ggctcccagc
    89581 aacctgacca tctggaaagg ttctgtttcg ctgaaggaag atccggcggg caactacatt
    89641 cactacgggg tgcgtgaatt tggcatgacc gctatcgcca acggcatcgc gcaccacggc
    89701 ggctttgtgc cgtataccgc gacgttcctg atgtttgttg aatacgcccg taacgccgcg
    89761 cggatggcgg cactgatgaa agcgcggcag attatggttt atacccacga ctcaattggc
    89821 ctgggcgaag atggtccgac gcaccaggct gttgagcaac tggccagcct gcgcttaacg
    89881 ccaaatttca gcacctggcg accgtgcgat cagatggaag cggcggtggg ctggaagctg
    89941 gcggttgagc gccacaacgg accgacggca ctgatcctct caaggcagaa tctggcccag
    90001 gtggaacgta cgccggatca ggttaaagag attgctcgtg gcggttatgt gctgaaagac
    90061 agcggcggta agccagatat tattctgatt gccaccggtt cagagatgga aattaccctg
    90121 caagcggcag agaaattagc aggagaaggt cgcaatgtac gcgtagtttc cctgccctcg
    90181 accgatattt tcgacgccca ggatgaggaa tatcgggagt cggtgttgcc ttctaacgtt
    90241 gcggctcgcg tggcggtgga agcaggtatt gccgattact ggtacaagta tgttggtctg
    90301 aaaggggcaa ttgtcgggat gacgggttac ggggaatctg ctccggcgga taagctgttc
    90361 ccgttctttg gctttaccgc cgagaatatt gtggcaaaag cgcataaggt gctgggagtg
    90421 aaaggtgcct gatggtgatc gccggatgct gtatgttccc ctcaccctaa ccctctcccc
    90481 agaagggcga ggggacggtc cgtgcgcttt ttagggccgc atctgcgatt ttgtttaacg
    90541 ggtgatccac aacgtgggcc tggcgtctgg cccatgccag ttatcgcagg taggttctgc
    90601 ggcgtaacgc accaggcgaa aacgctgacc gtcaaagcgc cagcgcgcct gaatgccaca
    90661 atcgcttaat ccgcgccctt tcgctaaggt caccagttca cgcgatttct catcaaatgt
    90721 tgcgttcatc agttccagtt cattcgtctc ctggccgttg ttgaacggca aacgcaaccg
    90781 aaccgggcgc gaggctagcg gctttttacg cgacacaatc catgccaaat caatggtgtt
    90841 ataggcccct gcctcacagc taatcatcat cagtgcttta tcatcggtca gcgcagtgac
    90901 attcacctca cgacgtaacg gatcaagcga gcagcgcaga ccattcatcc gccagtttcc
    90961 ataatccagc aaatcgttac gttcttcgag tgagagtggc gtcggcgttg ggttaaccac
    91021 cgcgacctct ttcagcgcag gcgcgggcgg tacgctgaga ggcggttcgt cccctttctt
    91081 gatccacgcg gtttcactgc caacgcgctt ttgctgagca tcaataaaca acaacgccgc
    91141 ttttaagccg ctcagagaaa tggtctgatt gccgtcgcgt aaggtgatcg ccttcccttc
    91201 ctgaattatc tgcaaaaacg cggtgatggt tgccgtatca tcggttacta ataaccatgg
    91261 tgaaatccgc cacttgtcac cacttaatgc caacggctca ccatctaata gcagccgtgg
    91321 cgctatctcc ccttcagacg catccggcga cttcaaccca ccgcgctcaa tacgtaaaac
    91381 ggcatcggta tgcgccccag cgctgcggct cagggtcatc accagtccat tatgatcgcc
    91441 cgtattgcgc gccacacaaa aattttgatt attacaggtg acctgccagt cggaaaacgc
    91501 ccgttgcgct ggtgccgccc acaccaaaga cgtcggcaac aaagcgaaaa aaaagagaag
    91561 gaaaatgcga tagcgcatgg acggtacgac cccagaagtt caaacaaaca gcacaagtcg
    91621 tatcttcgtt gcgcaggtag cgcggctcaa tcggatttgt cggattgagc caataataaa
    91681 caacgaaata ttattgctat ttttcagtcc attaaatgtg acatttgcaa atagttaagc
    91741 aataacaccg tcttaccgtc acgtatctcg ccggttttga tcatctccag cgcctggctg
    91801 aacggcagct cgagcacttc aatatcttca tcttcaacac cgccaccggc gttagcgcgc
    91861 tgattgtcac tgtattcggc gataaaaaag tggattagct cagtcacacc gccaggcgac
    91921 atatacagtt caaataattt gcgcacttcg cccacttcat agcccgtctc ttcaatcgct
    91981 tctttacgaa tgcacacttc cggttcgtcg ttatccagca gcccggcgca ggtttcaatc
    92041 agctgcccgc tttcattgcc attaacccag gtagcgacac ggaactgacg aatcagaacc
    92101 acgctctttt tcttcgcgtt gtacaggagg atcgtcgcac cattaccgcg atcgtaaact
    92161 tcacgtttat ggcggataac ttcgccatct ttgcgcgtga gatcgtaagt aatgttgtgc
    92221 agggtgaaat agttatcgga gagaattttg tctttaatga gggtgatttg ttgcgtcata
    92281 ccgactccac agcgcgaaat gaacaattat attacgctgt gaagtcgggt ttgtctgcgc
    92341 aggctatcag tgagatttga ctgattttac ccccagccag tcaataatcc cctgtgccgc
    92401 atgacgtcct tctgccattg cggtaaccac cagatccgca ccacgcacgg cgtcgccacc
    92461 ggcgaagatt ttcggattgg tggtctggta acggtactgg ctttccacat ccgcgatgat
    92521 gcggccccat ttgtctaccg ttacaccgtg cgactccagc cacggcatcc cgtgtggatt
    92581 gaagccaaac gccataatca ccgcatcggc tggcatgaca aattcactgc cctcaatcgg
    92641 tacaggtcgc cgacgcccct gggcatccgg ctcgccaaga cgcgtgcgca ggaaacgaat
    92701 tccgcaaacg tgaccttgct cattcagctc aagcgccacc ggctggacgt taaattcgaa
    92761 gttggccccc tcttcgcggg cgttcttcac ctctttcttc gagcctggca tgttagcttc
    92821 atcacgacga taagcgcagg tgacgttact cgcgccgtgg cgcagcgcag tacgcacaca
    92881 gtccatcgcg gtgtcgccgc cgcccagtac cacgacgtta agtccggcgg tattgataaa
    92941 cggctcttcc ggtagctctt cgagccccat cacctgttta gtgttggcaa tgaggaacgg
    93001 cagcgcgtca taaacgcccg gcgcatcttc attgggtaaa cccgctttca tggaacggta
    93061 agtgcctacg ccaacgaaga ccgcgtcgta ttgttccaaa agcaaatcca aagagacatc
    93121 tttacccact tcacaattga gttcgaagtg aatccccatc gcgctgaaga tttcccgacg
    93181 gcgtgccagc agggatttat ccagtttgaa agaaggaatg ccgaaagtga gcaagccacc
    93241 gatttctgga tggcgatcgt acaccgtcac cgccacgcca ttgcgggtca gaacatccgc
    93301 acaggccagc cctgccggac ctgccccgat aatcgccacc cgcttgtcca ctttggtgac
    93361 atgacttaag tcaggacgcc aacctttcgc caacgcctga tctgaaatat agcgttcaat
    93421 gttgccgata gttaccgcgc cgtgctcatc gcgaatagta caggcacctt cacacaaacg
    93481 gtcttgcgga caaacgcgtc cggtaatttc cggcagggtg ttggtctggt gagaaagctc
    93541 gacggcggcg tcgatgtttc cggctttcac cagttcaatc cactgcggaa tatggttatg
    93601 cagcgggcag gtccattcac aaacgctgtg ctcgccgaac ttaaggcagc gcgaggcttc
    93661 ccgttgtgcc tggtcggcgc gaaatggcag ataaatttca tcaaaaccgg ttttgcgcgc
    93721 ttcaatcgcc agtttatccg gctcgccacg cgcgggcgtt gcctgcattt gttcgacttt
    93781 actcattacc ggcatttctt gtgcagcggt actggcatgc cacggttgat gctcctgacg
    93841 tgcggtgcgc aagcggcggg atttcgccat gccggagagt gcgacgtcag tgaccagttg
    93901 cagcgcgtcc gccgggcaat tctcgacaca ggcaggaccg ttttcgcgcc ctgcacaaag
    93961 gtcgcattta tgcgccgtgg cttttacttt tcctgccgcg acgggtgtca ggacgatttg
    94021 catcgtacca aacggacagg ccaccacgca ggatttacag ccaatacact tttgctgatt
    94081 gacctgaatg ctgtcatcaa cgtggctgat tgcgccatta gggcagctac gggcgcaggg
    94141 cgcatcttca caatggtgac aggtcactgc actacgttgc tgctgatgtt tgataaccgt
    94201 aattcgggga tgaaaatggt gttggctcag gacatgttgc tcatcattgt gagccatgac
    94261 acaggcgatt tcacaagcat gacaacccag acattgctga ctgttggcca taataaaacg
    94321 attcataacg accttctttt ttggttgtaa aaaccttatt ctttatatga gtgttgttat
    94381 tacccgactt acaggggaac cggcaatgtt catgtgccca gaataagtaa ctatttcgct
    94441 ataaactgtg gcagatcaaa taatcccatc actgactaaa ttgcgtttca gcgaactgga
    94501 acattaatga ttttttgtgg agaagacgcg tgtgattgtt aaacgacccg tctcggccag
    94561 tctggcccgg gccttttttt acattgtgct gctgtcgatt ctttccacgg gtatcgctct
    94621 gctaactctg gcgagcagtt tgcgcgacgc tgaggctatc aatattgccg gatcgctgcg
    94681 tatgcagagt taccgcctgg gctacgactt gcaaagtggc agtccacaac tcaatgcaca
    94741 tcgccagttg tttcagcagg cactgcattc accggtatta accaacctca acgtctggta
    94801 tgtgccagaa gcagtaaaaa ctcgctatgc gcatctgaat gccaactggc tggagatgaa
    94861 taatcggctc agcaagggcg atttgccgtg gtatcaggcc aatattaata attatgttaa
    94921 tcagatagac ctgttcgtgc tggctttaca gcactacgct gaacgcaaaa tgctgctggt
    94981 ggtggcgatt tccctggctg gcggtatcgg tattttcacg ctggtctttt ttactttgcg
    95041 ccgcatacgc catcaggtgg ttgccccgct gaatcagctg gttaccgcca gtcagcgtat
    95101 tgaacacggg cagttcgact cgccgccgct ggataccagt ctgccgaatg agcttggtct
    95161 gcttgcaaaa acctttaacc aaatgtcgag cgagctgcat aaattgtacc tttcgctgga
    95221 ggcgtcagta gaagaaaaga cccgcgatct ccacgagacc aagcgtcgtc tggaggtgtt
    95281 gtatcagtgc agtgttccca ggcgctgaac accagccaga ttgatgtgca ttgtttccgc
    95341 catattttgc agattgttcg cgacaatgaa gcagctgaat atctggagct gaacgttggg
    95401 gataactggc ggattagcga agggaagcca aaccctgagc tgccaatgca gattttaccg
    95461 gtgacaatgc aagagacggt ttacggcgaa ctgcactggc aaaatagtca cgtttcatca
    95521 tcagaaccgc tgcttaacag tgtttcatcg atgctgggac gcggtttgta ctttaatcag
    95581 gcgcagaagc attttcagca gttattgttg atggaagaac gtgcgaccat cgcccgcgaa
    95641 ttgcacgact cgctggctca ggtgctttct tacttacgta tccagttgac gttactgaag
    95701 cgttcgatac cggaagataa cgccaccgca caaagtatca tggccgattt ttcccaggcg
    95761 ttgaatgatg cttatcggca gttacgcgag ctgttgacta ctttccgcct gacgctgcag
    95821 caggcggatc tcccctccgc gttgagggaa atgctggata cgttacaaaa tcaaaccagc
    95881 gctaaactga ccctcgactg ccgtctgcca accctggcac tggatgcgca aatgcaggtg
    95941 catttgttgc aaattattcg cgaagcggtg ctgaatgcga tgaagcacgc caacgccagc
    96001 gaaatcgccg tcagttgcgt caccgcgccg gacggcaatc acacggttta tatccgtgat
    96061 aacgggattg gtatcggtga accgaaagaa cccgaaggtc attatggact gaatatcatg
    96121 cgcgaacgcg cggaacggct gggcgggacg ctgacttttt cacaaccttc cggcggcggc
    96181 acgttagtga gtattagctt tcgctccgca gagggtgagg ggaaggggaa cacgccggct
    96241 gaggtgtaaa taattaccct cacaccggcc ctctcccttt aaaggcgaga gggaaaaacg
    96301 tgcccatatt gacctgtaag cgagtgtgtc agcaatctca tgataattta cattaactcc
    96361 tttttttctc cacgattggc tcgtaccttg ccgctacagt gaagcaagtc aagcctacaa
    96421 caatacgcag aaacacgagg tcctctttta atggcgaatt tctttattga tcgccccatt
    96481 tttgcctggg tgctgacaat cctgttgtgt ctgacaggta ccctggcgat tttttcattg
    96541 cccgttgaac aataccccga tctcgcgcca ccgaatgtgc gagtgaccgc taactatccc
    96601 ggcgcatcgg cccagacgct ggaaaacacc gtgacccagg ttatcgagca aaatatgacc
    96661 ggcctcgata atctgatgta tatgtcatct cagagcagtg gcaccggtca ggcatctgtc
    96721 actttaagtt ttaaagcagg caccgatccg gacgaagccg tgcagcaagt acaaaaccag
    96781 ctgcaatcag ccatgcgaaa gttaccgcag gcggtgcaaa atcagggcgt gacggtgcgt
    96841 aaaaccggcg ataccaacat tctgaccatt gccttcgtct ctaccgatgg ctcgatggat
    96901 aaacaggata ttgctgatta tgttgccagt aatattcagg acccgttaag ccgcgtgaat
    96961 ggcgtcgggg atatcgatgc ctatggttcg caatattcca tgcgtatctg gctggacccg
    97021 gcgaaactca acagtttcca gatgacggct aaagatgtca ctgatgccat tgagtcacag
    97081 aacgcgcaga ttgcggttgg gcaacttggt ggtacacctt ccgtcgataa gcaggcgctc
    97141 aacgccacca ttaacgccca gtcactgctg caaacaccag aacagttccg cgatatcacc
    97201 ttgcgggtga atcaggacgg ctcagaggta aggctgggcg atgtcgccac cgtcgaaatg
    97261 ggggcggaga aatacgatta tcttagccgc ttcaatggta agccagcctc cgggttgggg
    97321 gtaaaactgg cctccagcgc aaacgaaatg gcgacagcgg agctggtgct caatcgtctc
    97381 gacgagctgg cgcagtattt cccgcacgga ctggaataca aggtggcgta tgaaaccacc
    97441 tcgtttgtta aagcctccat tgaagacgtg gtgaaaacgc tgctggaagc tatcgctctg
    97501 gttttcctcg ttatgtatct gttcctgcaa aacttccgcg ccacgctgat acccactatc
    97561 gccgtgccgg tggtgttgat gggaaccttc tccgtacttt acgccttcgg ttacagcgtc
    97621 aacaccttaa ccatgttcgc gatggtgctg gcgatcggtc tgctggtgga tgacgccatc
    97681 gtggtggtgg aaaacgtcga acgtattatg agtgaggaag gactcactcc tcgcgaagcc
    97741 acacgtaaat cgatggggca gatccagggg gcactggtcg ggattgcgat ggtgctttcg
    97801 gcggtatttg taccaatggc cttcttcggc ggcaccaccg gtgccatcta tcgccagttc
    97861 tctattacca ttgttgcggc gatggtgctg tcagtactgg tagcgatgct cctcactccg
    97921 gctctgtgtg ccacgctact taagccactg aaaaaaggtg agcatcacgg gcaaaaaggt
    97981 ttttttgcct ggtttaacca gatgtttaac cgcaacgccg aacgctacga aaaaggggtg
    98041 gcgaaaattc tccaccgtag cctgcgctgg attgtgattt atgtcctgct gcttggcggc
    98101 atggtgttcc tgttcctgcg tttgccgacg tcgttcttac cgctggaaga ccggggcatg
    98161 tttactacct cggtacaatt gcccagcggt tcaacccaac aacagaccct gaaagtcgtt
    98221 gagcaaatcg agaaatatta cttcacccat gaaaaagaca acatcatgtc ggtgtttgcc
    98281 accgttggtt ctggccctgg gggtaacggg caaaacgtgg cgagaatgtt tatccgcctg
    98341 aaagactgga gcgaacgcga cagtaagacc ggcacctcgt ttgccattat cgagcgtgca
    98401 acgaaggcgt ttaaccaaat taaagaagct cgcgttatcg ccagcagccc accagcaatt
    98461 agcggtcttg gtagttctgc aggttttgat atggagttgc aggaccacgc tggagcgggt
    98521 cacgatgcgc tgatggcagc acgtaatcag ttgctggcgc tggcggcgga aaacccggag
    98581 ctaacccgtg tgcgccataa cggcctcgat gacagtccgc agttgcagat tgatatcgac
    98641 cagcgtaaag ctcaggcgct gggcgttgat atcgacgata ttaacgacac actgcaaacc
    98701 gcctggggtt cgagctatgt gaatgacttt atggatcgcg gtcgcgtgaa gaaagtctat
    98761 gtgcaggcag ctgcgccgta tcgcatgctg ccagatgaca tcaatctctg gtatgtccga
    98821 aataaagatg gcggcatggt gcccttctct gctttcgcga cctcacgctg ggaaacaggc
    98881 tcgccgcgtc tggaacgcta taacggttat tctgcggttg agattgttgg ggaagccgca
    98941 ccgggggtca gtaccggtac ggcgatggat attatggaat cgttagtgaa gcagctgcca
    99001 aacggctttg gtctggagtg gacggcgatg tcgtatcagg agcggctttc cggcgcgcag
    99061 gctccggcgc tgtacgccat ttccttgctg gtggtattcc tgtgtctggc ggcattgtat
    99121 gaaagctggt cggtaccgtt ctcggtaatg ctggtcgtgc cgctgggggt aatcggcgcg
    99181 ctgctggcaa cctggatgcg cgggctggaa aacgacgttt acttccaggt gggcctgtta
    99241 acggtcattg gtttatcggc gaaaaacgcc atcctgatcg tcgagtttgc taacgagatg
    99301 aaccaaaaag gccatgacct gtttgaagcg acgctccacg cctgccgtca gcgtttacgc
    99361 ccgattctga tgacctcgct ggcatttatc ttcggcgtat tgccaatggc aaccagcacg
    99421 ggtgccggtt ccggtggtca gcatgcggtg ggtactggcg taatgggcgg gatgatttca
    99481 gctactattc tggctattta cttcgtgccg ctgttctttg tgctggtgcg ccgccgcttc
    99541 ccgctgaagc cgcgcccgga ataagcaata aaaaaggcga catgccaatg tgtcgccttt
    99601 ttcaactttc cgattaagaa cctgctcagc gggttcttgc tgtttatact ttgtctcagg
    99661 aattacttac gaagcataac ttcgataaag tctttccagt tccccagttc acgttcaatc
    99721 ataacaacct ctcttataat tatgggtatt ctacggaaac aatataccgt ggtgaagcta
    99781 atttactcga ttgctgcgat gactacctcc gggggacaaa ccttatgtaa atactatggt
    99841 cctgcggtga taatttgtat gtgatacaca gcaacatttc gagatattca tacggcatct
    99901 aatactgatt taattctggt taaaatacag acagataaca agatgaatat tcttaatgtt
    99961 tacgttaaaa atgtttaata ttatttaata gttgttaatt tgaatacttc gataatgtta
   100021 tattttctga taatcatttg caggcaaaat gttttcaccc ttaaatgagt atttattctc
   100081 ataaatcgaa aaaggattca ttatggttac actttacggc atcaaaaatt gtgacaccat
   100141 taaaaaggct cgccgttggc tggaagccaa taacatcgac tatcgttttc atgattaccg
   100201 cgtcgatggg ctggacagcg aattattgaa cggttttatc aacgaattag gctgggaagc
   100261 gttactcaac acccgtggta caacctggcg taaactggac gaaactaccc gcaataaaat
   100321 caccgatgcg gcctctgcgg cggcattaat gactgaaatg cctgcaatta tcaaacgtcc
   100381 attgctctgc gcgcctggta agcctatgct gctgggtttc agtgattcca gttatcagca
   100441 atttttccat gaggtgtagt ctatgtcgtg cccggttatt gagctgacac aacagcttat
   100501 tcgccgccct tccctgagtc ctgatgacgc aggatgtcag gctttgttga ttgaacgttt
   100561 gcaggcgatc ggttttaccg ttgaacgcat ggattttgcc gatacgcaga atttttgggc
   100621 atggcgtggg caaggtgaaa cgttagcctt tgccgggcat accgacgtgg tgccgcctgg
   100681 cgacgccgat cgttggatca atccgccgtt tgaacccacc attcgtgacg gcatgttatt
   100741 cgggcgcggt gcggcagata tgaaaggctc gctggcggcg atggtggtag cagcagaacg
   100801 ttttgtcgca caacatccca accatacggg gcgactggca tttctgatca cttctgatga
   100861 agaagccagt gcccacaacg gtacggtaaa agtcgtcgag gcgttaatgg cacgtaatga
   100921 gcgtctcgat tactgcctgg tcggcgaacc gtcgagtatc gaagtggtag gtgatgtggt
   100981 gaaaaatggt cgtcgcggat cgttaacctg caacctcacc attcatggcg ttcaggggca
   101041 tgttgcctac ccacatctgg ctgacaatcc ggtacatcgc gcagcacctt tccttaatga
   101101 attagtggct attgagtggg atcagggcaa tgaattcttc ccggcgacca gtatgcagat
   101161 tgccaatatt caggcgggaa cgggcagtaa caatgttatt ccgggtgaac tgtttgtgca
   101221 gtttaacttc cgcttcagca ccgaactgac tgatgagatg atcaaagcgc aggtacttgc
   101281 cctgcttgaa aaacatcaac tgcgctatac ggtggattgg tggctttccg ggcagccatt
   101341 tttgaccgcg cgcggtaaac tggtggatgc ggtcgttaac gcggttgagc actataatga
   101401 aattaaaccg cagctactga ccacaggcgg aacgtccgac gggcgcttta ttgctcgcat
   101461 gggggcgcag gtggtggaac tcgggccggt caatgccact attcataaaa ttaatgaatg
   101521 tgtgaacgct gccgacctgc agctacttgc ccgtatgtat caacgtatca tggaacagct
   101581 cgtcgcgtga tgagtggttc tgcaagagga aataagcatg gactggctgg ctaaatattg
   101641 gtggattctg gtgattgtct ttttggtagg cgtcctgctg aacgtgatta aagatctcaa
   101701 gcgcgtcgac cataagaaat ttctcgccaa caagccggag cttcccccgc atcgtgattt
   101761 caacgataag tgggacgatg acgacgactg gccgaaaaag gatcaaccga agaagtaagc
   101821 tgagtgctca accgtaggcc ggataaggcg ttaacgccgc atccggcaat ggtgaacgat
   101881 ccctgatgcg acgctcacgc gtcttatcag gccaggtaat ctgtctttta catcatttca
   101941 atcacatcat catcgccagg cttaccgcca cttagcgctt ggtcgaaata atgtttcgga
   102001 atggtataac gcaagtgatc cagggcaaat tgcatactgc gattgtcgat cgcatggcct
   102061 aaatcctcca cgatatccag cgtcacgtca ccaccggcac tgattaacgc ttcctgcgcc
   102121 gcaactgcat gcgccagatc gataaccgga tcttcaccac catgaatcaa gtgaattgtc
   102181 gtggctgtag acgcggtctc cggcaggctc gcatagcgtc cgttaaaggc aatgacgcgc
   102241 gaagcaaggc ccggttcggc tttaatgctc tccagcgcca taatcgcgcc ttgtgagaaa
   102301 ccaattagcg ccgtggcgtt agcccctact ccgctctgtt tctgccagta gcgtaccgtt
   102361 tcaataaacg tcggcatgat ggcatccaca cgcgcctggc gattatcttc cgtaataccc
   102421 tgcactgaaa accactgacg ccccgccgga ttaccgctcg gctccgcgcc accgacactc
   102481 accaccagcg catcaggaaa cagcggtgca aaccagctgc ctatttcccc cattgccacc
   102541 gggttatccc cgacaccgtg gaaaagcagt aacaactgtt gtgcaggttt atccgggctt
   102601 tgaacaacaa aatggtcatg tttcatggcg aactccttaa ctgatgtcat taattttacg
   102661 ccgctgactc acaatgacca tgggagaaaa ttgaaggagt cagtgaaaaa attgccattg
   102721 cgttatgcga tcgcgcagac gctcagtgcg aacctcattt agtgcgaaca gcgcctgcgc
   102781 ggcctcttcc cgctggcgaa ccagtaacat cttgcggcct gaaagtttaa gtgtggtaca
   102841 taactgcgcg tcactggcgt ttttctgtaa acgcccacgc agcgccggta atgccagttc
   102901 actggtttgt agcagacgca ataagcaacc taacgatgtt aatagcggac gatgagcgaa
   102961 agcaaaaccg gccagttcaa gccagtcgtc gtcagaaagg atggcatcgt ttagtggatc
   103021 aacaggaagc atttcgccat tccactgcgc gagagcttgc gcatcgcgac gtaaacggta
   103081 atgctcacgt tcagccagct gtttacccgc atcactcatc ggtaacagcg ccatcgccgt
   103141 atagcaaccg ctgctggctt cccgatgatt acccatccgc accagcacaa aaccgcagcg
   103201 ttgccagaaa cgccataact ccccagtgta accaaaactc accgaaagat agtcgaggtc
   103261 atgcgtatat tgcaaagctc cagcaataag ttgtcgccct gtgccttccc gctgacgagc
   103321 cggatgaact gctatccggc taacccgcct tccgcgcaat gtcgccgcca gtggattgct
   103381 gccgtgcgcc gccagcgact gggccaccag attaccccgc gggcgacgaa aacctgccca
   103441 taccgcctga ctgagttctt gagataatcc gccctcatcc accagccaca acgccccggc
   103501 aatctcgttt tcgccagccg cctgtaaaaa atgttgccct ggtgcatcca tcatccggcg
   103561 taaatccagc ggcgaggtcc ggtagtgcgc accagacaat aactgataaa cctttaacgg
   103621 cgtttctggc tcgcttcgcc ataacgtttg ttcaaatgcg gaaatgacga tattgccttg
   103681 tggtgtatgg gtgaagtttt catcgttaaa aaccagtgcc tcactaacca ttttttccag
   103741 cgggcatccc tgtgcccagc ggatcggctg ttgcagttca aaacggtgta aatgcggaaa
   103801 gcgagcgcaa aatttcagca aaaaaccacg tccggtacct tcgtagccct gcaccgtagt
   103861 ggttaacaac gttcgaggaa aacgcgatac cagttgatgc aacaacggcg caggtatggc
   103921 tgcggcttcg tcgaccacca gccagtcggc ttgctcatcg ctggctaaca aggcatccgg
   103981 tgcaataaag cgaaacttct cgcccgcaaa ttgtgccagt acatccgttg ccgcttttgc
   104041 gggcgcggtg acaatcgcac tgcccgcaat acgagaaatg agttgccctg ccaacgccga
   104101 cttaccgcgc ccacgcgcag ccgttaccgc tgccacgccc ggcggcatgg tcagtagctg
   104161 ctgtaagagt tgctgttgtt ctggttgtgg tgcgccggtc gcggggtgcc agtcagtacg
   104221 gggagtaaaa tgcgccaacg agaacggctg gttttgccgc cagaggatag cgtcgttatc
   104281 cgccgtaagt acgcgtttga gatgctggac aaaatgcggc gtcgcaatag ggtcagggca
   104341 atcactccag cgcagcgagt cggcatcagg ttggttttcc cactcttccc atacagggag
   104401 taacaaaacc agccagcttc ccgctttcaa cgttccgcta agtgctgcaa aggcagcggc
   104461 atcaaagccg tggcgggcgt cgaataccgc atgccggaac tcgcgcccaa gtaaagtttg
   104521 tagcgccgag ggtgaacagt ggttttcagc atctagctgc ggcgaaatcc acagccagtc
   104581 gccaggtaag gcatcacgca acttaagagc atgatcaaaa caccaaccct cttccccgct
   104641 caacaccagc aagcggcaga tcccttcacg tttcatttgc gctgttaatg tgtaaagcgc
   104701 agtcagttca gccatccctg ccccggaaat taaatgcttt taccaaaagt attgcattgt
   104761 gccggatcgc cgctgtcgaa accacgttta aaccagctgt agcgttgctg agaagtgcca
   104821 tgtgtgaaac tgtctggtac tactcgcccc tgactttgct gttgtaaacg gtcatcgccg
   104881 atggcctgcg ccgcattcag cgcctcttcc agatcgccag tttccagaac gccttgctgc
   104941 tgcatactat gcccccagac accggcaaaa cagtcggcct ggagttccat acgcacagat
   105001 aagcggttta cttccgcctg cgtcgcgttt tgttgcagtt gacgaacttt cggctcgatg
   105061 cctaacagtt tctgcacatg atgaccgact tcatgggcga taacgtaccc ctgggcaaaa
   105121 tcgccatccg cgccaagttt gtctttcatg tcatcataga aggagagatc gatataaact
   105181 gtgccatccg ccgggcaata gaacggcccc ataatggact ggcccgcccc gcagccggtg
   105241 cgcgtcattc cacggtacat gaccagcttc ggttgctgat aggtcttacc catcttttcg
   105301 aactgttgtc cccaggtgtc ttccgtggtt gcctgaatca ccgaggtgaa ttttgcggct
   105361 tcgtcttcat tagggctaat tgaccgcgtt gattgttgtt gagaaaccgg ctgcccggtc
   105421 atcaacccgg ttaaatcaac gccatagtag cctgcaacca gcacgactat cagtaaaatc
   105481 agcccgcctt taccgctcgg cagacggaaa ccgggaccgc ccattgacgg accgccagag
   105541 ctgttgcgcc tgtcttcaac attgtcactt tcacgtcgcc cttgccaacg catagatacc
   105601 tcaacaatat attcattata gaaatgatcg taggtggttc agaggaagat taccacagga
   105661 aagcgggatg aaatggcgga ggaatgagcg ttcaatgcat atattccgag agcggttgtc
   105721 ttgccgtgcc agctgcacgg caagatgatg aacagaaaaa tcagtccagc tgtacaccca
   105781 ggcggcgggc gacggcttca taggcttcga tcaggccacc gaggctctgg cggaaacggt
   105841 ctttgtccat tttctccagt gtttctttgt cccacaggcg gctgccgtcc ggggagaact
   105901 catcgcccag taccacttca cctttgtaca gaccaaactc cagcttgaag tcgaccagaa
   105961 tcagaccagc atcatcgaac agttttttca gcacgtcgtt cgctttgtag gtcagctctt
   106021 tcatacgcgc caggttctct ttgctcaccc agccaaaggt ttcgcagtaa gattcgttga
   106081 ccatcggatc gtgcatggca tcgtttttca ggaacagatc gaacagcggc gggttcagct
   106141 caataccttc ttcgattcca agacgtttca ctagagagcc agcagcacgg ttacgcacga
   106201 cacactcaac cggcaccata tccagctttt tcaccagaca ttcggtatcg gagagcagac
   106261 gctccatttg agtcgggata cccgcttcag ccagtttgct cataatgaag tagttgaact
   106321 tgttgttcac catacctttg cgatcaaact gctcaatgcg cgcgccatcc cctgctgacg
   106381 tatcattgcg gaattcgagc accaacaggt ccgggttttc cgtgctgtat acggttttcg
   106441 ctttaccacg atacaactca gcttgctttt gcatctttat cactcctggg tgtgaattaa
   106501 cgttttaaaa tcttttgctg tctggtgtgc cggatgtttt gtcggatgcg gcgtgaacgc
   106561 cttatccgaa ctacgtccga caaacaaatt tcgtgcgaat taccgctaaa atcgcgtttt
   106621 cctgccgacg cacacgtttg cgtatcatat cagaaaaaag ggccggatga ttccagccct
   106681 gtatttttac ttgctaaacg cagcctggaa gacagctacc agcgcgtcgt tctgactctg
   106741 agtcagagta tgacctttcg gatcgatgaa ctgtaggctg ctgcggttat ctaaatcgcc
   106801 aacctgcagt ttatagtcac cggatgccag gcctggatcg ctcgcgccca gttcctgcca
   106861 gtcgctgtca gacagcggct tataagttac ggccatgttg ccctgcgaac gggtgctgtc
   106921 ggtcactttc atgcccactt tttccagcgc cgctggcaga cgttgccaaa ccacattgaa
   106981 cggcccgcgt acgaccagca ttggtaaacc ggtgtcatca gctgcacttt gtacgtccat
   107041 agtggtggag gcacgatttt gcgcagcgtt cgcggcgtca gtggcagatt tatccagacc
   107101 ggcggaaata acgttcatca tctccgtgct gtaacgctgc atggaagccg cgtctgcaac
   107161 cggtttgccc gcctgttcca ggttcagcag tttaaccgta accgcctgct gataaccctg
   107221 cggcttaaca gagatttgat aacgaccacg atactgctcg tcttcgtcca gacggttcca
   107281 ttgtacccaa tcggtggtca gtgtctgacc agcatcatca cgttgggtga tggtgtagtt
   107341 tttcgcctgc agcacgctaa ccacctgcgg ccacagagta ttgccacgac cattttccac
   107401 cagcaatgaa gcggtatcgc ccgtgaactg ggtacgcgcg ccagaaacca gtgccaacgg
   107461 ctgggctggt ggacgaatgt ccagcgcctt accgacagca ccactaccgt tggtcaccgg
   107521 gattgcataa tcaccggagg tcaccggcaa aatcattcca gccggggcat gaagctccgc
   107581 aagcggtgcc gcttccaggt aggcttcatc accactgacc tgacgcttat agcgtgagtc
   107641 agaactacag gcagcgagta ataaaacaag cgaaacaccc gcaacctttg ccaggcgcga
   107701 cttttgaaca gagtaagcca tcaaatctcc ctaaacttta cagcaaaccg gcatgcttaa
   107761 gcgccgctct gaccgtctca cgaccactgt cggtgattgg tgtcattggc aggcgcagcg
   107821 tatcggtcgc cacaagaccc agttccttac atgcccattt caccgggatt ggattgggtt
   107881 cgacaaatag tttgttgtgt aatggcatca gacgctgatt aataacgcgt gcctcggcaa
   107941 aatgcccttc tgctgccagt ttgcacatct gggccatatc acgcgctgcg acgttagccg
   108001 taacggaaat aactccatga ccgcccaatt gcatgaagtc cagcgcgctc gcatcatcgc
   108061 cgctcagcag aacaaaatca tctgaaacca gctctttgat ctggtttaca cgcgttaagt
   108121 tccctgttgc ctctttgatt ccgataatat tttttacttt cgccagacgg cccaccgttt
   108181 ccgggagcag atcgcagcca gtacgggacg gcacattata cagaatttgc ggcaggtcag
   108241 tatgctcagc gatggctttg aaatgctgat acaaaccttc ttgcgacgga cgattgtagt
   108301 aaggggttac cgtcaggcag ccgacgatac cactgtcatt gaagcgctgc gtcaggctaa
   108361 tggcttccgc agtagcgtta gcgccggtcc cggcaattac cggaatgcgc ccatcagcca
   108421 gatccagcgt catcatcacc acatcagcat gttcgtcatg atttaaggta gcggactcgc
   108481 cagtggtgcc aacagaaacg atcgccgaag taccgctggc gacatgataa tcaatcagtt
   108541 ttttcaagct agcccgacag acattacctt tttcatccat cggagtaaca atcgcgacaa
   108601 tacttcccgt gaacatgggc catcctctgt gcaaacaagt gtctcaatgg tacgtttggt
   108661 atggcattaa aagcaagcag acagaaccgt tctgattgtt gtatgcatga tttttttatg
   108721 ctttccttaa gaacaactca ccccttaaag gaataaccag tttgacactg tcatcgcaac
   108781 attatctggt gatcactgcg ttgggtgccg atcgccctgg aattgtgaac accatcaccc
   108841 gtcatgtcag tagttgcggc tgtaatattg aagacagtcg cctggcgatg ctgggagaag
   108901 agttcacgtt tatcatgctg ctttccggtt catggaatgc catcactctg attgaatcaa
   108961 cgttaccgtt gaaaggtgcc gaactggatc ttttaatcgt gatgaagcgc acgacggcgc
   109021 gtccgcgtcc gccaatgcca gcatctgtct gggttcaggt cgatgttgca gactccccgc
   109081 atttaattga acgcttcaca gcacttttcg acgcgcatca tatgaacatt gcggagctgg
   109141 tgtcgcgcac acaacctgct gaaaatgaac gggctgcgca gttgcatatt cagataaccg
   109201 cccacagccc cgcatctgcg gacgcagcaa atattgagca agcgttcaaa gccctatgta
   109261 cagaactcaa tgcacaaggc agtattaacg tcgtcaatta ttcccaacat gatgaacagg
   109321 atggagttaa gtaatgaatc cactgaaagc cggtgatatc gcaccgaaat ttagcttgcc
   109381 ggatcaagac ggagaacaag ttaatttgac cgacttccag ggacagcgtg ttctggttta
   109441 tttctacccg aaagccatga cccccggctg taccgtacag gcctgcggct tacgcgataa
   109501 catggatgag ttgaaaaaag cgggcgttga tgtgctgggt atcagcaccg ataaacccga
   109561 aaaactctcc cgttttgcgg aaaaagagct gcttaacttt acgctcctgt ctgatgagga
   109621 ccaccaggtg tgcgaacaat tcggcgtctg gggtgaaaag tccttcatgg gcaaaaccta
   109681 cgatggtatt catcgcatca gcttcctgat tgacgctgat ggcaaaatcg aacatgtctt
   109741 tgacgatttc aaaaccagca atcaccacga cgttgtgctg aactggctga aagaacacgc
   109801 ctgattactt tgctccattc cgtgctggct gcgcttgcgg ccagcatacc tcacttctcg
   109861 tgatcaagat cacattctcg ctttcccctg cgacacgggt gtcgaatcca ttttttgctg
   109921 aacgttaatg accatcattt ttgtaccgtt cagaatccag ttaatacata acttattgaa
   109981 tagattgagt taatcagaat ggcatccttt atacaatatg aaatgcaatg tttcatatca
   110041 ttttcaagga gccgacatga accgctttgt ggtggccgaa ccactgtggt gtacaggatg
   110101 taatacctgt ctcgctgcct gttcggacgt gcataaaacg caaggtttac agcaacaccc
   110161 gcgcctggcc ctggcgaaga cgtcaacaat cactgcccct gtcgtgtgtc atcactgtga
   110221 ggaagcccct tgcctgcagg tctgcccggt caatgccatc tctcagaggg atgatgcgat
   110281 ccaactcaac gaaagcctct gtattggctg caagctttgc gccgtggtct gcccatttgg
   110341 cgcaatcagc gcttcaggaa gccgtccggt gaatgcccat gcgcaatatg tttttcaggc
   110401 tgaaggctca ctcaaagacg gcgaagaaaa cgtgccgccc caacatgcgt tgctgcgctg
   110461 ggaaccgggt gtccagaccg tcgcggtgaa atgcgacctg tgtgatttct tgccagaagg
   110521 tccggcctgc gttcgcgctt gcccgaatca ggcgttacgg ctgatcaccg acgatagcct
   110581 gcaacgtcag atgaaagaaa aacagcgcct tgccgcaagc tggtttgcca atggcgggga
   110641 gtctcccctt tccctcactc aggagcaaca ctaatggatg ccctgcaatt attaacctgg
   110701 tcgctgatcc tctatctgtt tgccagtctg gcttcgctgt ttttactcgg tctggacaga
   110761 ctggctatta agctttccgg catcacatcg ctggtgggcg gcgtgattgg catcatcagc
   110821 ggaattacgc aattacatgc tggtgtaact ttagtcgccc attttgccac cccttttgac
   110881 tttgccgatt taaccctgcg aatggatagc ctctcggcat ttatggtgct agttatctcc
   110941 ttgctggtgg tggtttgttc gctctattca ttgacttata tgcgcgaata cgagggcaaa
   111001 ggcgcggcgg cgatgggctt ctttatgaat cttttcatcg catcgatggt tgccctgctg
   111061 gtgatggaca acgctttttg gttcatcgtg ctgtttgaaa tgatgtcgct gtcttcctgg
   111121 tttctggtca ttgccaggca ggataaaacg tcgatcaacg ctggcatgct ctactttttt
   111181 atcgcccacg ccggatcggt gctgattatg atcgccttct tgctgatggg gcgcgaaagc
   111241 ggcagcctcg attttgccag tttcgcacgc tttcactttc tccggggctg gcgtcggcgg
   111301 tgtttctgct ggcctttttc ggttttggcg cgaaagccgg gatgatgccg ttgcacagct
   111361 ggttgccgcg cgctcaccct gccgcaccat cgcacgcttc ggcgttgatg tctggcgtaa
   111421 tggtcaaaat cggtattttc ggcatcctga aagtagcgat ggatctgctg gcgcaaacgg
   111481 gtttgcctct gtggtggggc attctggtga tggcgatcgg cgcaatctcc gcgctcctgg
   111541 gcgtgctata tgcgctggcg gaacaggata tcaaacggct gctggcctgg agtaccgtcg
   111601 aaaacgtcgg cattattttg ctggcagtcg gtgtggcgat ggacggtctg tcactgcacg
   111661 acccgctgct caccgtggtt ggactgctcg gcgcactgtt tcatctgctc aaccatgcgc
   111721 tgttcaaagg gctgctattt ctcggcgcgg acgcgattat ttcgcgtttg catacccacg
   111781 acatggaaaa aatgggggca ctggcgaaac ggatgccgtg gacagccgca gcatgcctga
   111841 ttggttgcct cgcgatatca gccattcctc cgctgaatgg ttttatcagc gaatggtaca
   111901 cctggcagtc gctgttctca ctaagtcgtg tggaagccgt agcgctacaa cttgcgggtc
   111961 ctattgctat ggtaatgctg gcagtcactg gtgggctggc agtaatgtgc ttcgtcaaaa
   112021 tgtacggtat tactttctgt ggtgcgccgc gcagtacaca cgctgaagag gcacaggaag
   112081 tgccaaatac gatgatcgtc gccatgctac tgctcgcggc actctgcgta ttaattgcgc
   112141 ttagtgccag ttggctggca ccgaagataa tgcatattgc ccatgcgttt accaataccc
   112201 ctcccgtcac tgtcgccagc ggaatagcac ttgtacccgg cacgtttcat acacgggtca
   112261 ctccttcatt actgttgctg ttactactgg cgatgccttt gctgccaggc ctttactggc
   112321 tgtggtgtcg ttcgcgccgc gcagcgtttc gtcgcacagg agatgcctgg gcatgcggct
   112381 acggctggga aaacgcgatg gctccgtcag gcaatggcgt gatgcagccg ctgcgtgtgg
   112441 tcttctctgc gttatttcgt ctacgacaac agctcgaccc tacgctgagg ctaaataaag
   112501 gtcttgcgca cgtcaccgcc agggctcaga gcacagaacc cttctgggat gagcgggtga
   112561 tccgccccat cgtgagcgcc acccaacggc tggtcaaaga aatacagcat ctgcaaagcg
   112621 gcgactttcg tctctattgc ctgtatgtgg tcgccgcact ggttgtgctg ctaatcgcta
   112681 ttgccgtcta aggaaatcac catgagacaa actctttgcg acggatatct ggtcattttt
   112741 gcgttagcac aggccgtgat tctgctgatg ctaaccccac tttttacggg tatttcccgg
   112801 cagatacgcg cgcgtatgca ctcccgccgc gggccgggga tctggcagaa ttatcgcgat
   112861 atccacaaac tgtttaaacg ccaggaagtt gcgccgacat cttcaggtct gatgttccgc
   112921 ctgatgccgt gggtattaat cagcagcatg ctggtgctgg cgatggcctt accactattt
   112981 attaccgttt ccccttttgc gggcggcggc gatctgatca cccttatcta tcttcttgcc
   113041 ctgtttcgtt ttttctttgc tctttccggg ctggataccg gaagtccgtt tgcgggagtc
   113101 ggtgccagtc gcgagttgac gctcggcatt ctggtcgaac caatgcttat tctctcactg
   113161 ctggtattgg cgctgatagc aggttccacg catatcgaga tgatcagcaa tacgctggct
   113221 atgggctgga actcgccgct aaccaccgta ctggcgttac tggcctgtgg ttttgcctgc
   113281 ttcattgaga tgggaaaaat tccctttgat gttgctgaag cagaacagga attacaggaa
   113341 ggcccgctga ccgaatattc cggtgccggg ctggcgctag cgaaatgggg gctggggctg
   113401 aaacaggtcg tgatggcagc actgtttgtg gccctgtttc tgccctttgg gcgcgcgcaa
   113461 gaactttctc tcgcctgcct gctgacttca cttgtcgtta cgctgctcaa ggttttgctg
   113521 atttttgtac tggcctcaat cgcagaaaac acgctggcac gcgggcgttt tttactcatt
   113581 caccatgtga cctggcttgg cttcagcctt gctgcgcttg catgggtctt ttggttaacc
   113641 ggtctgtaag gagcactgac ggaatatgga aaatcttgct ctgacgacgt taatgctgcc
   113701 ttttatcggc gcactggtcg tttcgttttc gccacaacgt cgggccgccg aatggggggt
   113761 tttgttcgcc gcgctgacca cgctgtgcat gttgtcgctg atctccgcgt tttatcaggc
   113821 cgataaagtt gccgtcacgt tgacgttggt caacgtgggg gatgtggcat tatttggcct
   113881 ggtcattgat cgcgtgagta cgctgattct gtttgtggtg gtgtttctcg gtttgctggt
   113941 cacgatctac tccacgggtt atctgacgga taaaaatcgc gaacacccgc ataacggcac
   114001 gaatcgttat tacgcatttt tgctggtgtt tatcggcgcg atggcgggac tggtactctc
   114061 ctcgacgctg ctcggtcagt tgttgttttt tgaaattacg ggcggctgct cctgggcgtt
   114121 gatcagttat taccagagcg ataaagcgca gcgttcagca ctaaaagcgt tgcttatcac
   114181 ccatatcggt tcgttggggt tgtatcttgc cgccgccacg ctgtttttgc agaccggaac
   114241 gtttgtgctt agcgcgatga gcgagttaca tggcgacgca cgttatctgg tttatggcgg
   114301 catcctgttt gccgcgtggg ggaaatcggc ccagctaccg atgcaagcgt ggctaccgga
   114361 cgcaatggaa gcgccaacac cgatcagcgc ctatctccac gccgcatcga tggtgaaagt
   114421 gggcgtttac atttttgccc gcgccattat cgacggcggc aatatcccgc atgtgattgg
   114481 cggcgttggc atggtcatga cactggtcac cattctttat ggctttctga tgtatttgcc
   114541 acagcaggat atgaagcggt tgctggcctg gtcgaccatc actcaacttg gctggatgtt
   114601 cttcggcttg tcgctctcca tcttcggctc gcggctggcg ctggagggta gcatcgccta
   114661 catcgtcaac cacgcgttcg ctaaaagcct gtttttcctt gtagcaggtg cgctgagtta
   114721 cagctgcggc acgcgcttgt tgccgcgtct gcgtggcgta ttgcacaccc tgccgttgcc
   114781 aggcgtggat ttctgcgtgg cagcgctggc aattaccggt gtgccgccgt tcaacggctt
   114841 cttcagtaaa ttcccgctgt ttgccgccgg ttttgcgttg tcagtggagt actggatcct
   114901 gctgcccgcc atgattctgc tgatgattga atcggtcgcc agtttcgcct ggtttattcg
   114961 ctggtttggt cgcgtcgtgc cgggcaaacc gagcgaggcc gtcgccgatg ccgcaccgct
   115021 gccagggtca atgcgcctgg tgttgattgt actgattgtg atgtcgctga tttccagcgt
   115081 aatcgccgcg acctggttgc agtaaggaga tgatgaatga ccggttctat gatcgtaaat
   115141 aatctggcgg gactgatgat gctgacatcg ctgtttgtga ttagcgtcaa aagctatcgc
   115201 ctgtcatgcg gattttacgc ctgccagtcg ctggtgctgg tgtctatttt cgccactctc
   115261 tcgtgcctgt tcgccgcaga gcaactgctg atctggtccg ccagcgcctt tatcaccaaa
   115321 gtgctgctgg taccgttaat catgacttac gctgcacgaa atattcccca gaacatcccg
   115381 gaaaaagcgt tattcggtcc ggcaatgatg gcactgctcg cggcgttaat tgtcctgctt
   115441 tgcgcatttg tcgttcagcc cgtgaagcta ccgatggcta ccgggctgaa accggcgctg
   115501 gcggtagcgt taggtcattt tctgcttggc ctgctgtgca ttgtcagcca acgcaatatc
   115561 ctgcggcaaa tttttggtta ctgcctgatg gaaaacggct cccatctggt gctggcgctt
   115621 cttgcctggc gagcaccgga actggtggaa ataggtatcg ctaccgacgc catcttcgcc
   115681 gtcattgtga tggtgttact ggcaagaaaa atatggcgta cccacggcac gctggacgtg
   115741 aacaacttga ccgcgctgaa gggataatga gatgagttat tctgtgatgt tcgctttact
   115801 cctgctcacg ccgctgcttt tttcgctgct ctgttttgcc tgccggaaac gggggctctc
   115861 tgcgacttgt acggtgactg tattacatag cttagggatc acactgctgc tgattctagc
   115921 actctgggtg gtccaaactg ccgctgatgc aggagaaata ttcgctgcgg gactgtggct
   115981 tcatattgat ggtctgggcg gtttgttcct cgccattctt ggtgtgattg gctttctcac
   116041 cggtgtttac tcgattggct acatgcgtca tgaagtggca cacggcgagc tttcacccgt
   116101 tacactgtgc gattactacg gtttcttcca tctgtttttg ttcaccatgc tgctggttgt
   116161 taccagcaat aacctgattg tgatgtgggc ggcgatcgaa gccaccacct taagctcggc
   116221 gtttctggta ggcatttacg gtcagcgttc atcgctggaa tcagcatgga agtacatcat
   116281 tatttgtact gttggtgtcg cttttggtct gttcggtacc gtgctggtat acgccaacgc
   116341 cgccagcgtt atgccgcagg cagaaatggc gatattctgg agcgaggttc ttaagcaatc
   116401 gtccttgctt gacccaacat taatgctgtt ggcctttgtg tttgtgctaa ttggctttgg
   116461 taccaaaacc gggctatttc ccatgcacgc ctggctgccg gatgctcaca gtgaagcgcc
   116521 gagtccggtc agcgccctgc tctccgccgt attgctgaac tgcgcgctgt tggtgctgat
   116581 tcgctattac atcattattt gccaggccat cggcagcgat ttccccaacc ggttgttgct
   116641 catcttcggc atgttgtcgg ttgccgtggc ggcatttttc attctggtac agcgggacat
   116701 taagcgtctg ctggcgtact ccagcgtgga gaacatgggg ctggtcgcgg tggcgctagg
   116761 cattggcggg ccgctgggaa tttttgccgc gctgctgcac accttaaacc acagtctggc
   116821 aaaaacgctg ctgttctgcg gttccggcaa tgtactgctc aagtacggca cgcgcgatct
   116881 caacgtcgtc tgtgggatgc tcaaaatcat gccatttacc gccgtgctgt ttggtggcgg
   116941 tgcgctggcg ctggcgctgg cgctggcagg gatgccgccc ttcaacattt ttcttagcga
   117001 atttatgacc gttaccgcag gactggcacg taatcacctg ctgattatcg tcctgctgtt
   117061 attgctgtta acgctggtgc tggcgggcct ggtacggatg gctgcgcggg tgttaatggc
   117121 gaaaccgccg caggccgtta accggggtga tctcggctgg ttgaccacct cgccaatggt
   117181 gattctgctg gtcatgatgc tggcgatggg aacgcatatt ccacaacctg tcatcaggat
   117241 cctggcgggc gcttccacta tagtcctctc agggacgcac gacctgcctg cacaacgtag
   117301 cacctggcat gattttttgc cttcaggcac cgcatctgtt tcggagaaac acagtgaacg
   117361 ttaattcatc gtcaaatcgt ggcgaagcga ttctcgccgc cctgaaaacg cagttccccg
   117421 gcgcggtgct ggatgaagag cgacaaacgc ctgaacaggt caccattacg gtgaaaatca
   117481 atctgctgcc tgacgttgta cattatcttt attatcaaca tgatggctgg cttccagtcc
   117541 tgtttggcaa cgacgagcgg acacttaacg gtcattacgc ggtttattat gccctttcaa
   117601 tggaaggggc cgaaaaatgc tggatcgtgg tgaaggcact ggtcgatgcc gacagtcggg
   117661 agtttccgtc agtcacaccg cgcgtccctg ccgcggtctg gggcgagcga gaaattcgcg
   117721 atatgtacgg gctgattccg gttggcctgc cggatcagcg tcgcctggtg ttgcccgatg
   117781 actggccgga agatatgcat ccgctgcgca aagatgcgat ggattatcga ctgcgccctg
   117841 aaccgacgac agattccgaa acgtatgcgt ttatcaatga gggcaacagc gatgcgcggg
   117901 tgatccctgt cggcccgctg catatcacct cctatgaacc gggtcacttc cgcttgtttg
   117961 tggatggcga gcaaattgtc gatgctgatt accgcctgtt ttatgtccat cgcggcatgg
   118021 agaaactggc agaaacgcgg atgggctaca acgaagtgac cttcttatcg gaccgcgtgt
   118081 gtgggatttg cggttttgcc cacagtgtgg cctataccaa ctcggttgaa aatgcactgg
   118141 ggattgaggt gccgcaacga gcacatacta ttcgctcgat tctgctggaa gtcgagcggc
   118201 tacacagtca tttgctcaac cttggcctct cctgccattt tgttggtttt gataccggct
   118261 ttatgcaatt tttccgcgtg cgggaaaagt cgatgacgat ggcggaattg ctgaccgggt
   118321 cgcgtaaaac ctacggtctg aatctgattg gtggtgttcg ccgcgatatt ctcaaagagc
   118381 aacgtctgca aacgctgaaa ctggtgcgcg agatgcgcgc cgacgtgtcg gagctggtag
   118441 agatgctgct tgctacgccg aatatggaac aacgcactca gggcattggc attctcgacc
   118501 gacaaatcgc ccgtgattat agccctgtag ggccgctgat ccgcgcagtg gttttgcccg
   118561 tgatttgcgc tttgatcacc cctacgccga ctacggcaat attccaaaaa cactgtttac
   118621 ctttaccggc ggcgatgttt tctcccgcgt gatggtccgt gtcaaagaga cgtttgattc
   118681 gctggcaatg ctggaatttg ccctcgacaa catgccggat accccactgc tgaccgaagg
   118741 ctttagctat aaacctcacg cattcgcgct gggctttgtt gaagcgccac gcggtgaaga
   118801 cgtgcactgg agcatgctcg gtgataacca aaaattgttc cgctggcgct gtcgtgccgc
   118861 cacctacgcc aactggccgg tgttgcgtta catgctgcgc ggcaataccg tttctgacgc
   118921 accgctgatt atcggtagcc ttgatccctg ctactcctgt accgaccgtg tgacgctggt
   118981 agatgtgcgc aagcgccagt caaaaaccgt gctgtataaa gagatcgaac gctacggcat
   119041 tgatcgtaac cgttcgccgc tgaagtaagg acagaagatg ctgaagttac tgaaaactat
   119101 tatgcgcgcc ggaaccgcga cggtgaaata tcccttcgcg ccactggagg tcagcccagg
   119161 ctttcgcggg aaaccggacc tgatgcccag ccaatgtatt gcctgcggtg cctgcgcctg
   119221 tgcttgtccg gcaaatgcgc tgactatcca gaccgacgac cagcaaaatt cgcgcacctg
   119281 gcagctctat ctggggcgtt gtatttactg cggacgttgt gaagaagtgt gcccgaccag
   119341 agccatccag cttaccaata actttgaact gaccgtcacc cataaagccg atctctatac
   119401 ccacgcgacg ttccatctac aacgttgcag ccgttgcgaa cgcccgtttg ccccgcaaaa
   119461 aaccgtcgca ctggctgctg aattgttggc acagcaacaa aatgctccac aaaaccgcga
   119521 aatgttatgg gcgcaagcga gcgtctgccc ggagtgcaaa caacgcgcaa cgctgatcaa
   119581 cgacgataca gatgtaccgc tggtggctaa ggagcagcta tgagtccagt gcttacgcaa
   119641 tatgtcagcc agcccatcac gctggacgag caaacgcaaa agatgaagcg gcatttgcta
   119701 caggatatcc gtcgctcggc ttacgtttat cgcatcgatt gcggcggctg caacgcctgt
   119761 gaaatcgaaa tttttgctgc catcaccccc gtattcgacg cagaacgttt tggcattaag
   119821 gttgtttcat caccgcgtca cgccgatatt ttgttattta ctggcgcagt cacccgggcg
   119881 atgcgtatgc ctgcacttcg ggcgtatgag tctgcccccg atcataaaat ctgtgtttcc
   119941 tacggcgcgt gcggtgtcgg cggcggtatt ttccacgatc tctacagcgt ctggggcggc
   120001 agcgacacca ttgtccccat tgatgtttgg atccccggct gcccgccaac accggccgcc
   120061 accattcacg gtttcgccgt ggcgctcggt ttgctgcaac agaagattca cgcggtggat
   120121 tatcgcgatc ccaccggggt gactatgcaa ccgttgtggc cgcagatccc gccatcacag
   120181 cgtatcgcca ttgagcgaga agcgcggcgg ctggcgggct atcgtcaggg gcgagaaatt
   120241 tgcgatcggc tcctgcgcca tttaagcgac gatcctacag gaaatcgggt taacacctgg
   120301 ttgcgcgatg ccgacgatcc acgtctcaat agtatcgttc agcaactctt tcgcgtactc
   120361 cgggggttac atgactgaag agtgcgggga aattgttttc tggacgctgc gaaaaaagtt
   120421 tgtcgccagt agcgacgaga tgccggaaca cagctctcag gtaatgtatt actcgctggc
   120481 tatcggtcat cacgttggcg tgattgattg tctgaatgtc gccttccgct gcccactgac
   120541 ggaatacgaa gattggcttg cactggtcga agaggagcaa gcccgacgta agatgctggg
   120601 ggtgatgact tttggtgaga ttgttattga cgccagccac accgccctgt tgacccgggc
   120661 attcgcgcca ctggcagatg acgcgacgtc tgtgtggcaa gcgcgtagca ttcaattcat
   120721 tcatctgttg gatgaaattg tgctggaacc ggctatctat ctgatggcca gaaaaattgc
   120781 gtgagaagga tttctcatta ataaggactg ttgatggcta tgtcagacga agcgatgttt
   120841 gccccgccgc aaggaataac aattgaagcg gtaaacggaa tgctcgcgga gcggttagcg
   120901 cagaaacacg gcaaggcgtc tttattacgc gccttcatcc cgctgccgcc gccgttcagc
   120961 ccggtacaac ttattgaact gcatgttctc aaaagcaact tctattaccg ctaccatgat
   121021 gatggcagcg atgtgacggc aacaacagag tatcagggcg agatggtcga ttattcgcgt
   121081 tacgccgtcc ttctcggcag tagtggaatg gcggagctac gctttattcg tacccacggc
   121141 agccgtttta ctccccagga ttgcacactg tttaactggc tggcacgcat tatcaccccg
   121201 gttctgcaat catggctcaa tgatgaagca cagcaggtgg cgctgcgttt gctggagaaa
   121261 gatcgcgatc atcatcgggt actggtggat atcactaatg cagtgctgtc acatcttgat
   121321 ctcgacgatc tgatcgctga cgtcgctcgt gagatccatc attttttcgg tctggcttca
   121381 gtcagtatgg tactgggcga tcatcgaaag aacgagaagt tcagcctgtg gtgcagcgat
   121441 ctttctgcct cacattgtgc gtgtctgcca cacaatatgc ctggcgacag tgtattgctg
   121501 acacaaacgc tacaaacccg acaaccgacc ttgacgcacc gtgcagatga tctgtttctc
   121561 tggcaacgcg acccgttatt actcttactt gcatctaacg gctgcgaatc tgcgctcctt
   121621 ataccgctta cctttggcaa ccatacaccg ggtgcattgt tgctggcgca tacctcttcc
   121681 actctcttta gtgaggaaaa ctgccagcta ctacaacaca tagccgatcg catcgctatt
   121741 gccgttggca atgccgatgc ctgccgtaga atgaccgatt tgcaggaaag tttgcagcaa
   121801 gaaaaccacc agcttagcga gcagctcctt tcgaatctgg gcgtcggtga cattatctat
   121861 caaagccagg caatggaaga cctgctccag caggtagata ttgtggcgaa gagcgacagt
   121921 acggtgttga tttgcggtga aaccggaacc ggcaaagagg tgatcgccag agcgatccat
   121981 caacttagcc cgcgacacga caagccgctg gtcaaaatca actgcgctgc catccccgcc
   122041 agtcttctgg aaagtgagtt attcggtcat gacaaagggg cgtttactgg tgcgattaat
   122101 acccatcgtg gtcgttttga aattgccgat ggcggcacgt tgtttctcga tgaaattggc
   122161 gatctgccgt tagaacttca gcctaaactg ctgcgcgtat tgcaggaacg ggagattgag
   122221 cgtctcggcg ggagtagaac gatcccggta aatgtcagag tcattgccgc caccaaccgt
   122281 gatttgtggc aaatggttga agatcgccag tttcgcagcg atctctttta tcgcctgaat
   122341 gtcttcccac tggaattgcc tccgctgcgc gaccgtccgg aagatatccc tcttttagca
   122401 aagcatttca cgcaaaaaat ggcgcgccat atgaatcgtt caattgacgc catcccgacc
   122461 gaggcgctac gccagttgat gtcgtgggac tggccgggca acgtgcgcga gctggaaaac
   122521 gtgattgagc gagcggtact gctgactcgc ggtaacagtc tgaatttaca tcttaatgtc
   122581 cgacaaagcc gtttactgcc gacgctaaat gaagattcag cgcttcgcag ttcaatggcg
   122641 tagttgctgc acccgacgac gccagagaat gacgaagaag aacgtcagcg cattgttcag
   122701 gtattgcgag aaaccaatgg cattgttgcc gggccccgtg gcgcggcgac acgattaggg
   122761 atgaagcgca ccacgctgct gtcacgaatg cagcgtctgg gaatctcggt tcgcgaggtg
   122821 ttgtaatctg cttttgcagg agtatgcatg agaaacaaac tctctttcga cttgcagttg
   122881 agcgccagaa aagcggcaat cgctgaacgg attgccgccc ataaaattgc ccgcagtaaa
   122941 gtgtcggtct ttttaatggc gatgtccgct ggcgtgttta tggcgatgtc cgctggcgtg
   123001 tttatggcga tcggatttac tttttacctt tccgttatcg ccgatgcccc gtcttcatag
   123061 gcattaaccc atctggtggg cggcctttgc tttacacttg gctttatttt gctggcggtt
   123121 tgcggcacca gcctgttcac ctcgtcggta atgacggtaa tggcaaaaag tcggggcgtt
   123181 attagttggc gaacttggct gattaacgca cttctggtgg cctgcggtaa tctggcaggt
   123241 attgcctgtt tcagtttgtt aatctggttt tccgggctgg tgatgagtga aaacgcgatg
   123301 tggggagtcg cgattttaca ctgcgccgag ggcaaaatgc atcatacatt tactgaatct
   123361 gtcagcctcg gcattatgtg caatctgatg gtttgcctgg cgctgtggat gagttattgc
   123421 ggacgttcgt tatgcgacaa aatcgtcgcc atgattttgt ccatcaccct gtttgtcgcc
   123481 agtggctttg agcactgtat cgccaatttg tttgtgattc cgttcgccat tgccattcgc
   123541 catttcgccc ctacctcctt ctggcaactg gcgcacagta gcgcagacaa ttttccggca
   123601 ctgacggtca gccattttat taccgccaat ctgctcccgg tgatgctggg taatattatc
   123661 ggcggtgcag tgctggtgag tatttgttat cgggctattt atttacgtca ggaatcctga
   123721 aaaatcagcc cggcgaaaca gttcgtcggg ctgaagatta ttcttgcgcg atttgcccat
   123781 cgggccaggc gtggatcacg gctttgatca gcgtcgccaa tggaatggcg aagaatacgc
   123841 cccagaatcc ccacaaacca ccgaagatca ccaccgataa aataatcacc agcggatgca
   123901 ggttaaccgc ttcggagaac aacaccggta ctagcaggtt gccgtccagc gcctgaataa
   123961 tcagatacac cgcgaagcag ctccagaatt cagtacctgc gccaaactgg aatagcgcca
   124021 cgccaaccac cggaatggtc accacaaatg cgccaatgta cggaataaga accgagaagc
   124081 cgaccagcac tgccagcagc agcgaatagt tcagcccgaa gagcaagaac cccagccagg
   124141 tggcgatccc caccacgatc atctccagca ctttgccgcg gatatagttg gtgatttgtt
   124201 gattcatctc cttccacacc tgtcctgcca gcccacggtt acgcggcagc acccggcgaa
   124261 cggcgttcag catctgctct ttgtctttca gcaggaagaa gaccatcaat ggcaccagca
   124321 ccagatagac ggctatggtc agcaaaccga ccagcgaggc gagggaaatt ttcaccaccg
   124381 aatcgcccat ggtcagcatc cgactgcgca tattttcggc cattgcatca ataatgcccg
   124441 cgtccattaa caccggatag cggcgcggca acgtggcggc aaagtcagaa agcttattga
   124501 gcatccccgg catatcgcgg attaagtaga tgccctgttg ccaggcgata ggcagtacca
   124561 cgaacgccat cagtagcaat ataccgacaa aaaccaccaa tacaatcgac gtcgcccagc
   124621 ggcgggagca gccaatagat tgcaggcgca cggttggcca ttccagcaaa taagccagca
   124681 caatagccac cagcaacgga gcaagcaggc cactaaagaa aaagataatg ccaaatccgg
   124741 caactaaaat aaccagcaag gcaatcgctt ccgggtcgct aaaacggcgg cgataccatt
   124801 gcatcaacat ttcgagcata aagaccttcc ctgaacctca agagcgggat tgcgatccgc
   124861 aattgtatcg aaatgtcaca aaaaagactt cgctttttat gacggattca ggaaactgaa
   124921 aagtcatttg agtgggctaa tcttcgccgt tacactcaaa ggcggcgagg tgggaacgat
   124981 atttcacagt atcggtcaaa tgactatctt cagaaataca ggatagaggt tatgttcagg
   125041 cagttgaaaa aaaacctggt tgcaaccctc attgctgcta tgaccattgg tcaggtagcc
   125101 ccggcgtttg ccgacagcgc agacaccttg ccggatatgg gaacctccgc aggaagcacg
   125161 ctttccattg gtcaggaaat gcagatgggc gactattatg tccgccagct acgcggcagc
   125221 gcgccgttaa ttaatgaccc gctgttaacg caatatatta attcgctggg gatgcgtctg
   125281 gtttcgcatg ccaattcggt taagacaccg tttcattttt ttctgatcaa caacgacgaa
   125341 attaacgcct ttgctttctt tggcggcaac gtggtgctgc actctgccct gttccgttat
   125401 tccgataacg aaagtcaact ggcttcagtt atggcgcacg aaatctccca cgtcacccaa
   125461 cgtcacctgg cgcgagcgat ggaagatcaa caacgcagcg cgccgctgac ctgggtcggc
   125521 gcgttaggtt ctattttact ggcgatggcc agtccgcagg cggggatggc ggcgctgacc
   125581 ggtacactgg cgggaacgcg ccaggggatg atcagtttca cccagcaaaa tgaacaggaa
   125641 gcggaccgca ttggtattca ggtgctgcaa cgctcgggat tcgatccaca ggcgatgccg
   125701 accttcctgg aaaaattgct cgatcaggcg cgttactcct cgcgcccgcc ggaaatttta
   125761 ttgactcacc cgttgccgga aagtcgtctg gcagatgccc gcaaccgtgc taatcagatg
   125821 cgcccgatgg tggtgcagtc gtcggaagat ttctatctgg cgaaagcgcg cacactgggg
   125881 atgtataatt ccggacgtaa ccagctcacc agtgatttgc tggatgaatg ggcgaaagga
   125941 aacgttcgtc agcaacgagc ggcgcaatat ggtcgtgctt tacaggcgat ggaagccaat
   126001 aaatacgacg aggcgcgtaa aacgctgcaa ccgttactgg cggcagaacc tggcaacgca
   126061 tggtatctcg atctggctac cgatatcgat cttgggcaaa acaaagccaa tgaggcaatc
   126121 aatcgcctga aaaatgcccg cgatttacgc accaatccgg tgttgcagct caacctggcg
   126181 aacgcttatc tgcaaggcgg tcaaccacaa gaagcggcca atattctgaa tcgctacacc
   126241 tttaataata aagatgacag caacggctgg gatttgctgg cacaggcgga agccgcgcta
   126301 aataaccgcg atcaggagct ggctgcgcga gcagaaggtt atgcgctcgc cggacgactc
   126361 gatcaggcca tttcgctgtt gagtagcgcc agttcgcagg tgaaattagg cagcctgcaa
   126421 caagcgcgtt acgatgcgcg catcgaccag ttgcgccagc tgcagaaacg ctttaagcct
   126481 tataccaaga tgtaattcgc attaaaggaa gaacaatgac caaacaggta aaaatctacc
   126541 ataacccacg ctgttcaaag agccgggaaa cgctgaacct gctgaaagaa aacggcgtgg
   126601 agccagaagt ggtgctctac cttgagacac ccgccgatgc ggcaacgctg cgcgatttgc
   126661 tgaaaatgct ggggatgaat agcgcccgtg aattgatgcg ccagaaagag gatctttata
   126721 aagaactaaa cctggctgac agttcgctta gcgaagaggc gctgattcag gcaatggtgg
   126781 ataatccgaa gctgatggag cgcccgatta tggtcgcgaa tggcaaagcg cggattggtc
   126841 gtccaccgga gcaggtactg gaaatcgtgg gttaagatgt gctccatcgc tagttgaagc
   126901 acattgccgg atgcgacgct tgccacgtct tatccggcct acgaatacta tggattaact
   126961 tcgcgtaggt caggcgttcg cgccgcatcc gacaataaac accttatcta caacttcaga
   127021 atttctttca caaacggaat ggtcagctta cgttgcgcgg taatcgacgc acgatccaac
   127081 tgatccaacg tcataaatag cgtgcgcatt tctctgtcga gccgcttcag caagaaacgc
   127141 cccacatctt ccggcagttc aaaaccacgc aaacgcgcgc gtaactgtag cgcctgcaac
   127201 ttatcttcat cagaaagtgg ctgcaatttg tagatctgcc cccagtcgag acgcgacgcg
   127261 agatccggta atcccagatt caactgccgc ggtggacgat cgccggtgat caacaaccgt
   127321 gttttgcccg attccagaat tcgattgtag agatcgaaaa tcgccatctc ccacaactca
   127381 tcgcctgcaa tacactcaat gttgtcgata cagaccagcg acaaatgctc cataccgtcg
   127441 agcacttccg gaacaaacca ggtgcgttta tccagcggga catagcccac cgcatcgcca
   127501 cgctgcgaca attccgcgca agccgcgtgc agcagatggc tgcgccccgc gccttcgcgt
   127561 gcccagagat agatgtaacc gctatgttcc tgacgcagca cgttttgcag cgcggccagt
   127621 aaagaggagt tatcccccgg ccagaaactt gcaaaggttt cgtcgtcagg aagataaagt
   127681 ggcaaagaga gctgtgccgg tgtgttcaga gatacctcaa ccaggatttc acaaaatcgc
   127741 gagaagttta ccacagaatc ccatgatgtt tgaaccgggc agcaacactg cccggtcggt
   127801 acattatttg tctgttatat ccgcgtcttc tgcgtccagc accacttctt cagggcgcag
   127861 cacactgatc agtttgaaaa tcaaacttaa gccgataccg acgatggtcg ccagcgccat
   127921 accttttaac tccgccgcgc cgatgtttac cttcgcgcca ctgacgccga tgatcaaaat
   127981 cacggaagtc aggatcaggt tctgtgcttt gttgtaatcc actttcgatt cgatcaaaac
   128041 acgaataccg gaagcaccaa tgacaccata aagcagcagc gaaacgccgc ccatcaccgg
   128101 caatgggatc atctggatag cggcagccag tttaccgacg caggaaagca ggatagcgaa
   128161 aatcgccgcc ccgccgataa cccaggtact gtaaacacgg gtgatcgcca tcacgccaat
   128221 gttttctccg taagtggtat ttggcgtaga gccaaagaag ccggagatca cggtcgacag
   128281 gccgttagca aacatcgaac ggtgcagacc tggatcgcgc agcagatctt ttttgacgat
   128341 attagccgtt actaccaggt gccctacgtg ttcggcaata accactaacg ccgctggcag
   128401 aatagtcaga atggcaaacc actcgaagcg cggcgtatag agggttggca gtgcaaacca
   128461 gtgagcatta ataatcggcg tggtatcgac aattcccatt gcgaaagaga gcgcgtaccc
   128521 caccagcacg ccaattaaaa tcgggataat tgccaggaaa ccacgaaaca gcacggaacc
   128581 taaaaccgtg accgccaggg tggtaataga gatgatgatg gttttggagt ttggcgtttg
   128641 cccttcagcc gggagtaaac ccgccatacc ggcagctacg cccgccagct ccagaccgat
   128701 gacggcaacg attgcgccca ttgccgcagg tggaaacagc acgtccaacc agccggtccc
   128761 cgctttcttc acgataaaag aaaccaggca gaacagcacg ccgcacataa taaagccgcc
   128821 cagcgcgact tcatacccta acggcaacag taacaatacc ggtgaaataa aggcaaagct
   128881 ggaaccaaga taagccggaa ttttcccttt acagatgaag agatacagca gcgttccaat
   128941 accgttaaat aacagtacag tcgccgggtt aatatgaaat aagacgggca ccaggacggt
   129001 tgcaccaaac atggcgaaca aatgttgcaa actaagcggg attgtctgta aaagtggcgg
   129061 tctttcactc accccgatag cacggcgcgt catagtatta tcctctgtat tatgtgttat
   129121 aggcgcttta ctcaaaaaaa agccgactct taaagtcggc tttaattatt tttattcttt
   129181 atttcgtacc aaagattttg tcaccggcat cgccgaggcc cggaataatg tatccgtgct
   129241 cgttcagtcc ctggtcaatc gatgcagtat acagttcgac gtccgggtgc gctttttcca
   129301 gcgcagcgat accttctggc gcagcgacca gcaccagaac tttgatgctg ctgcagcccg
   129361 cttttttcag caggtcgatg gtcgcgataa cggaaccacc ggttgccagc attgggtcaa
   129421 cgatcagcgc catacgctca tcgatgttag aaaccagttt ctggaagtac ggtaccggct
   129481 ccagcgtttc ttcattacgg tacataccga caacgctgat gcgcgcgctc ggaacgtttt
   129541 ccagcacacc gtccatcata ccaagacccg cacgcagaat tggcacaacg gtaattttct
   129601 tacctttgat ctggtcgatt tctaccgggc cgttccagcc ttcgatagtt actttttccg
   129661 tttcgaggtc ggcggtcgct tcgtaagtca gcaggctacc cacttcggaa gcgagttcgc
   129721 gaaagcgctt ggtgctgata tcttgctcac gcatcagtcc cagcttgtgt ttgacgagtg
   129781 ggtgtttgac ttccacgatc ttcatactct ttctcctttg aggggcagcc acaaaaaaat
   129841 cgacggatta tacctccttt cttcagggcg gcaatattat tttcgttgac tttagtcaaa
   129901 atgataacag tttgagataa agttatttta tattcaaatg gttatgaaag aagattattc
   129961 catccgaaaa ctaacctttg ccctggcaca agtcttcttt cgccgcgcgc ccggggaaaa
   130021 gacgtgcaaa aaggttgtgt aaaacagtct cgcaaacgtt tgctttccct gttagaattg
   130081 cgccgaattt tatttttcta ccgcaagtaa cgcgtgggga cccaagcagt gaccgataaa
   130141 acctctctta gctacaaaga tgccggtgtt gatattgacg cgggtaatgc tctggttgga
   130201 agaatcaaag gcgtagtgaa gaaaacgcgt cgtccggaag tgatgggcgg tctgggcggc
   130261 ttcggtgcgc tgtgtgcatt gccgcaaaaa tatcgtgaac ccgtgctggt ttccggcact
   130321 gacggcgtag gtaccaagct gcgtctggca atggacttaa aacgtcacga caccattggt
   130381 attgatctgg tcgccatgtg cgttaatgac ctggtggtgc aaggtgcgga accgctgttt
   130441 ttcctcgact attacgcaac cggaaaactg gatgttgata ccgcttcagc ggtgatcagc
   130501 ggcatcgcgg aaggttgtct gcaatcgggc tgttctctgg tgggtggcga aacggcagaa
   130561 atgccgggga tgtatcacgg tgaagattac gatgtcgcgg gtttctgcgt gggcgtggta
   130621 gaaaaatcag aaatcatcga cggctctaaa gtcagcgacg gcgatgtgct gattgcactc
   130681 ggttccagcg gtccgcactc gaacggctat tcgctggtgc gcaaaattct tgaagtcagc
   130741 ggttgtgatc cgcaaaccac cgaacttgat ggtaagccat tagccgatca tctgctggca
   130801 ccgacccgca tttacgtgaa gtcagtgctg gagttgattg aaaaggtcga tgtgcatgcc
   130861 attgcgcacc tgaccggcgg cggcttctgg gaaaacattc cgcgcgtatt gccagataat
   130921 acccaggcag tgattgatga atcttcctgg cagtggccgg aagtgttcaa ctggctgcaa
   130981 acggcaggta acgttgagcg ccatgaaatg tatcgcacct tcaactgcgg cgtcgggatg
   131041 attatcgccc tgcctgctcc ggaagtggac aaagccctcg ccctgctaaa tgccaacggt
   131101 gaaaacgcgt ggaaaatcgg tatcatcaaa gcctctgatt ccgaacaacg cgtggttatc
   131161 gaataatgaa tattgtggtg cttatttccg gcaacggaag taatttacag gcaattattg
   131221 acgcctgtaa aaccaacaaa attaaaggca ccgtacgggc agttttcagc aataaggccg
   131281 acgcgttcgg ccttgaacgc gcccgccagg cgggtattgc aacgcatacg ctcatcgcca
   131341 gcgcgtttga cagtcgtgaa gcctatgacc gggagttgat tcatgaaatc gacatgtacg
   131401 cacccgatgt ggtcgtgctg gctggtttta tgcgcattct cagcccggcg tttgtctccc
   131461 actatgccgg gcgtttgctg aacattcacc cttctctgct gccgaaatat cccggattac
   131521 acacccatcg tcaggcgctg gaaaatggcg atgaagagca cggtacatcg gtgcatttcg
   131581 tcaccgatga actggacggt ggcccggtta ttttacaggc gaaagtcccg gtatttgctg
   131641 gtgatacgga agatgacatc accgcccgcg tgcaaaccca ggaacacgct atttatccac
   131701 tggtgattag ctggtttgcc gatggtcgtc tgaaaatgca cgaaaacgcc gcgtggctgg
   131761 atggtcaacg tctgccgccg cagggctacg ctgccgacga gtaatgcccc cgtagttaaa
   131821 gctccagctc tgccgctggc gtttttcaat tcacctgtaa atcgcaagct ccagcagttt
   131881 ttttccccct tttctggcat agttggacat ctgccaatat tgctcgccat aatatccagg
   131941 cagtgtcccg tgaataaaac ggagtaaaag tggtaatggg tcaggaaaag ctatacatcg
   132001 aaaaagagct cagttggtta tcgttcaatg aacgcgtgct tcaggaagcg gcggacaaat
   132061 ctaacccgct gattgaaagg atgcgtttcc tggggatcta ttccaataac cttgatgagt
   132121 tctataaagt ccgcttcgct gaactgaagc gacgcatcat tattagcgaa gaacaaggct
   132181 ccaacgctca ttcccgccat ttactgggca aaattcagtc ccgggtgctg aaagccgatc
   132241 aggaattcga cggcctctac aacgagctat tgctggagat ggcgcgcaac cagatcttcc
   132301 tgattaatga acgccagctc tccgtcaatc aacaaaactg gctgcgtcat tattttaagc
   132361 agtatctgcg tcagcacatt acgccgattt taatcaatcc tgacactgac ttagtgcagt
   132421 tcctgaaaga tgattacacc tatctggcgg tggaaattat ccgtagcgat agcatccgtt
   132481 acgcgctgct ggagatccca tcagataaag tgccgcgctt tgtgaattta ccgccagaag
   132541 cgccgcgtcg acgcaagccg atgattcttc tggataacat tctgcgttac tgccttgatg
   132601 atattttcaa aggcttcttt gattatgacg cgctgaatgc ctattcaatg aagatgaccc
   132661 gcgatgccga atacgattta gtgcatgaga tggaagccag cctgatggag ttgatgtctt
   132721 ccagtctcaa gcagcgttta actgctgagc cggtgcgttt tgtttatcag cgcgatatgc
   132781 ccaatgcgct ggttgaagtg ttacgcgaaa aactgactat ttcccgctac gactccatcg
   132841 tccccggcgg tcgttatcat aattttaaag actttattaa tttccccaat gtcggcaaag
   132901 ccaatctggt gaacaaacca ctgccgcgtt tacgccatat ttggtttgat aaagcccagt
   132961 tccgcaatgg ttttgatgcc attcgcgaac gcgatgtgtt gctctattat ccttatcaca
   133021 cctttgagca tgtgctggaa ctgctgcgtc aggcttcgtt cgacccgagc gtactggcga
   133081 ttaaaattaa catttaccgc gtggcgaaag attcacgcat catcgactcg atgatccacg
   133141 ccgcacataa cggtaagaaa gtcactgtgg tggttgagtt acaggcgcgt ttcgacgaag
   133201 aagccaacat tcactgggcg aagcgcctga ccgaagcagg cgtgcacgtt atcttctctg
   133261 cgccggggct gaaaattcac gccaaactgt tcctgatttc acgtaaagaa aacggtgaag
   133321 tggtccgtta cgcacacatc gggaccggga actttaacga aaaaaccgcg cgtctttata
   133381 ctgactattc gttgctgacc gcagatgcgc gtatcaccaa cgaagtacgg cgggtattta
   133441 actttattga aaacccatac cgcccggtga catttgatta tttaatggtg tcaccgcaaa
   133501 actctcgccg tctgttatat gaaatggtag accgcgaaat cgccaacgcg cagcaagggc
   133561 tgcccagtgg tatcaccctg aagctaaata accttgtcga taaaggcctg gttgatcgtc
   133621 tgtatgcggc ctccagctcc ggcgtaccgg ttaatctgct ggttcgcgga atgtgttcgc
   133681 tgatccccaa tctggaaggc attagcgaca acattcgtgc catcagtatt gttgaccgtt
   133741 accttgaaca tgaccgggtt tatatttttg aaaatggcgg cgataaaaag gtctaccttt
   133801 cttccgccga ctggatgacg cgcaatattg attatcgtat tgaagtggcg acaccgctgc
   133861 tcgatccgcg cctgaagcag cgggtgctgg acatcatcga catattgttc agcgatacgg
   133921 tcaaagcacg ttatatcgat aaagaactca gtaatcgcta cgttccccgc ggcaatcgcc
   133981 gcaaagtacg ggcgcagttg gcgatttacg actacatcaa atcactcgaa caacctgaat
   134041 aaccctatgc caatacacga taaatcccct cgtccgcagg agtttgctgc ggtcgatctt
   134101 ggttcaaaca gttttcacat ggtcatagcc cgtgtggtag atggtgccat gcagattatt
   134161 ggccgcctga aacagcgggt gcatctggcg gacggcctgg ggccagataa tatgttgagt
   134221 gaagaggcaa tgacgcgcgg tttgaactgt ctgtcgctgt ttgccgaacg gctacaaggg
   134281 ttttctcctg ccagcgtctg tatagtcggc acccatacgc tgcgtcaggc gctgaacgcc
   134341 actgactttc tgaaacgcgc ggaaaaggtc attccctacc cgattgaaat tatttccggt
   134401 aatgaagaag cccgtctgat ttttatgggc gtggaacata cccaaccgga aaaaggtcgc
   134461 aaactggtta ttgatattgg cggcggatct acggaactgg tgattggtga aaatttcgaa
   134521 cctattctcg ttgaaagccg ccggatgggt tgtgtcagct ttgcccagct ttattttcct
   134581 ggcggggtca tcaataaaga gaattttcag cgcgctcgca tggcggcagc acaaaaactg
   134641 gaaactttaa cctggcaatt ccgtattcag ggctggaacg ttgcaatggg cgcttccggt
   134701 accataaaag ccgcccatga agtgttaatg gaaatgggcg agaaagacgg gataattacc
   134761 ccggaacgtc tggaaaaact ggtaaaagaa attttacgtc accgtaattt cgcatcgctg
   134821 agtttaccgg gtctttccga agagcggaaa acagtcttcg ttcctggact ggcgatttta
   134881 tgtggtgtgt ttgatgcttt agccatccgt gaactgcgcc tttctgacgg ggcgttacgc
   134941 gaaggcgtac tgtatgaaat ggaaggacgt ttccgtcatc aggatgtgcg tagtcgcacc
   135001 gccagcagcc tcgccaacca gtatcacata gacagcgaac aggcccgacg agtgctggat
   135061 accactatgc aaatgtacga acagtggcgg gaacagcaac cgaagctggc gcatccgcaa
   135121 ctggaggcgc tactgcgatg ggccgccatg ctgcatgagg tcgggttgaa tatcaaccac
   135181 agcggtttgc atcgccactc cgcttatatt ctgcaaaaca gtgacttgcc gggttttaat
   135241 caggaacagc agctgatgat ggcgacactg gtgcgctatc accgtaaagc gattaagctc
   135301 gacgatctgc cgcgctttac cttgtttaag aagaaacagt tcctgccact gatacagcta
   135361 ttgcgccttg gcgtattact aaacaatcaa cgtcaggcaa ccaccacacc gccaacattg
   135421 acactgatta ctgatgacag tcactggaca ctgcgtttcc cgcatgactg gtttagtcag
   135481 aatgcgctgg tactgcttga tctggaaaag gagcaagaat actgggaagg cgtggctggc
   135541 tggcggttga aaattgaaga agaaagtacc ccagaaatcg ccgcttaatg ctttgcgggc
   135601 cgacgagaaa tgtcggcccg cattattcag gcactttcgc gaatgggttc gatttcattc
   135661 agcgtatcaa ttaacggttg cggcttacca ataagataac cctgcatata atcgatcccc
   135721 aaagagagca ccgcctcgcg gatctcttcg ttttcaacgt actctgccac tacccgcatt
   135781 ttcttcattc gcgccagatg gcaaatcgac gccacaatct gataatccag actatttgac
   135841 acaatattgc ggataaagct gccgtcaatt ttaagcagat cggcattcac atttttaagc
   135901 cgtgcatagc tggcgtagcc ggtgccgaaa tcatcaatcg caatctggca gcctaattcc
   135961 tgaagatgct gcaaggtgat ttgcgcctgc ttaacattgg tcagagcatt actttcggtg
   136021 acttcaaaaa taagttgcca cgcttcaatc tgatatttag ccagcaactg actgacttca
   136081 acaggaaaac gagcctgaca taccgaggtt ggagacagat taatagcaaa acggtgagcg
   136141 ggcatcttcg ctctgttttc agccataaat tgcagcgtat gctcaatgac ccacatgtcg
   136201 atactcgacg ataaaccaaa ttcgtgcgcg accggcaaga agctaccggg gccgatcagt
   136261 tcatcattct cacctttcat gcgcagaaga atttcatggt aaacatcacc acgcataccg
   136321 gtaatcggct gggccatcag gaaaaaatgg ttgtgttcca gcgcctgctg tagtcgattc
   136381 atcatcgcga ctttatcttt caattcgcgt tgcaaataca ttgccccgca acgctgcata
   136441 ttttccgggg cgttggtcac gatggaaagt tcggcgaccg tatttagctc tcccagcagc
   136501 aggtagatat gattcactgg cgagcgcaca tagcagtaac tgacgccaat ctgcggttgc
   136561 atcggcatcc catcccaaaa gaaacgaaat tgcttgagat gactatccag tgcggtaatg
   136621 cgctcctggt gcgattctgt attcagtcgc agcgcgagat cgttacccga aagctgataa
   136681 acatcttcac ccggttccag caagggtgac agccagtgag aaagtttttg ctt
//
LOCUS       DAJTRD010000002        95544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTRD010000002 DAJTRD010000000
VERSION     DAJTRD010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.002"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO315_000132"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO315_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5889197.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="OO315_000133"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="OO315_000133"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5889198.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="OO315_000134"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="OO315_000134"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5889199.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="OO315_000135"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="OO315_000135"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5889200.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="OO315_000136"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="OO315_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5889201.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="OO315_000137"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="OO315_000137"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5889202.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="OO315_000138"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="OO315_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5889203.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="OO315_000139"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="OO315_000139"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5889204.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="OO315_000140"
     CDS             complement(8942..9223)
                     /locus_tag="OO315_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889205.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="OO315_000141"
     CDS             complement(9237..10898)
                     /locus_tag="OO315_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5889206.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="OO315_000142"
     CDS             complement(10882..11208)
                     /locus_tag="OO315_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5889207.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLEQYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="OO315_000143"
     CDS             complement(11264..11446)
                     /locus_tag="OO315_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889208.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="OO315_000144"
     CDS             complement(11430..11870)
                     /locus_tag="OO315_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5889209.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="OO315_000145"
     CDS             complement(11870..12166)
                     /locus_tag="OO315_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5889210.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="OO315_000146"
     CDS             complement(12163..12501)
                     /locus_tag="OO315_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5889211.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="OO315_000147"
     CDS             complement(12498..13709)
                     /locus_tag="OO315_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5889212.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="OO315_000148"
     CDS             complement(13711..14283)
                     /locus_tag="OO315_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5889213.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="OO315_000149"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="OO315_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15781..16005)
                     /locus_tag="OO315_000150"
     CDS             complement(15781..16005)
                     /locus_tag="OO315_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889214.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNITDYRAFLETGALIEKDHHG
                     FVRCALSGEMLAVNPEQTDALIEFLKGIRD"
     gene            complement(16147..16308)
                     /locus_tag="OO315_000151"
     CDS             complement(16147..16308)
                     /locus_tag="OO315_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5889215.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(16318..16623)
                     /locus_tag="OO315_000152"
     CDS             complement(16318..16623)
                     /locus_tag="OO315_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889216.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16620..16898)
                     /locus_tag="OO315_000153"
     CDS             complement(16620..16898)
                     /locus_tag="OO315_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889217.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(17187..18935)
                     /locus_tag="OO315_000154"
     CDS             complement(17187..18935)
                     /locus_tag="OO315_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5889218.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18932..19231)
                     /locus_tag="OO315_000155"
     CDS             complement(18932..19231)
                     /locus_tag="OO315_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5889219.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(19237..19470)
                     /locus_tag="OO315_000156"
     CDS             complement(19237..19470)
                     /locus_tag="OO315_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889220.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19472..19692)
                     /locus_tag="OO315_000157"
                     /pseudo
     CDS             complement(19472..19692)
                     /locus_tag="OO315_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19685..19906)
                     /locus_tag="OO315_000158"
     CDS             complement(19685..19906)
                     /locus_tag="OO315_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889221.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19896..20096)
                     /locus_tag="OO315_000159"
     CDS             complement(19896..20096)
                     /locus_tag="OO315_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5889222.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            20226..20405
                     /locus_tag="OO315_000160"
     CDS             20226..20405
                     /locus_tag="OO315_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889223.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20530..20859
                     /locus_tag="OO315_000161"
     CDS             20530..20859
                     /locus_tag="OO315_000161"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889224.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20909..21097)
                     /locus_tag="OO315_000162"
     CDS             complement(20909..21097)
                     /locus_tag="OO315_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5889225.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21472..22701)
                     /locus_tag="OO315_000163"
     CDS             complement(21472..22701)
                     /locus_tag="OO315_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5889226.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22950..24071
                     /gene="roxA"
                     /locus_tag="OO315_000164"
                     /gene_synonym="ycfD"
     CDS             22950..24071
                     /gene="roxA"
                     /locus_tag="OO315_000164"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5889227.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="OO315_000165"
     CDS             complement(24120..25346)
                     /gene="pepT"
                     /locus_tag="OO315_000165"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5889228.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25596..26732
                     /gene="potA"
                     /locus_tag="OO315_000166"
     CDS             25596..26732
                     /gene="potA"
                     /locus_tag="OO315_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5889229.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26716..27573
                     /gene="potB"
                     /locus_tag="OO315_000167"
     CDS             26716..27573
                     /gene="potB"
                     /locus_tag="OO315_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5889230.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27570..28364
                     /gene="potC"
                     /locus_tag="OO315_000168"
     CDS             27570..28364
                     /gene="potC"
                     /locus_tag="OO315_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5889231.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            28361..29407
                     /gene="potD"
                     /locus_tag="OO315_000169"
     CDS             28361..29407
                     /gene="potD"
                     /locus_tag="OO315_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5889232.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="OO315_000170"
     CDS             complement(29563..30384)
                     /gene="cobB"
                     /locus_tag="OO315_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5889233.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="OO315_000171"
     CDS             complement(30400..31311)
                     /gene="nagK"
                     /locus_tag="OO315_000171"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5889234.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="OO315_000172"
     CDS             complement(31340..32584)
                     /gene="lolE"
                     /locus_tag="OO315_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5889235.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="OO315_000173"
     CDS             complement(32584..33285)
                     /gene="lolD"
                     /locus_tag="OO315_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5889236.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="OO315_000174"
     CDS             complement(33278..34477)
                     /gene="lolC"
                     /locus_tag="OO315_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5889237.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34739..35812
                     /locus_tag="OO315_000175"
     CDS             34739..35812
                     /locus_tag="OO315_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5889238.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35940..39386
                     /gene="mfd"
                     /locus_tag="OO315_000176"
     CDS             35940..39386
                     /gene="mfd"
                     /locus_tag="OO315_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5889239.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39529..40491
                     /gene="ldtC"
                     /locus_tag="OO315_000177"
     CDS             39529..40491
                     /gene="ldtC"
                     /locus_tag="OO315_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5889240.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40574..40786)
                     /locus_tag="OO315_000178"
     CDS             complement(40574..40786)
                     /locus_tag="OO315_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5889241.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            41071..41703
                     /gene="comR"
                     /locus_tag="OO315_000179"
     CDS             41071..41703
                     /gene="comR"
                     /locus_tag="OO315_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5889242.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41765..42304)
                     /locus_tag="OO315_000180"
     CDS             complement(41765..42304)
                     /locus_tag="OO315_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5889243.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42531..43835)
                     /gene="ndh"
                     /locus_tag="OO315_000181"
     CDS             complement(42531..43835)
                     /gene="ndh"
                     /locus_tag="OO315_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5889244.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(44243..44785)
                     /locus_tag="OO315_000182"
     CDS             complement(44243..44785)
                     /locus_tag="OO315_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5889245.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44808..45833)
                     /gene="nagZ"
                     /locus_tag="OO315_000183"
     CDS             complement(44808..45833)
                     /gene="nagZ"
                     /locus_tag="OO315_000183"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5889246.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45844..46668)
                     /gene="thiK"
                     /locus_tag="OO315_000184"
     CDS             complement(45844..46668)
                     /gene="thiK"
                     /locus_tag="OO315_000184"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5889247.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46649..47290)
                     /gene="lpoB"
                     /locus_tag="OO315_000185"
     CDS             complement(46649..47290)
                     /gene="lpoB"
                     /locus_tag="OO315_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5889248.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(47305..47682)
                     /locus_tag="OO315_000186"
     CDS             complement(47305..47682)
                     /locus_tag="OO315_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5889249.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47685..48044)
                     /gene="hinT"
                     /locus_tag="OO315_000187"
     CDS             complement(47685..48044)
                     /gene="hinT"
                     /locus_tag="OO315_000187"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5889250.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48383..50568
                     /gene="fhuE"
                     /locus_tag="OO315_000188"
                     /pseudo
     CDS             48383..50568
                     /gene="fhuE"
                     /locus_tag="OO315_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50628..52061)
                     /gene="ptsG"
                     /locus_tag="OO315_000189"
     CDS             complement(50628..52061)
                     /gene="ptsG"
                     /locus_tag="OO315_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5889251.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(52356..53153)
                     /locus_tag="OO315_000190"
     CDS             complement(52356..53153)
                     /locus_tag="OO315_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5889252.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(53164..54168)
                     /gene="holB"
                     /locus_tag="OO315_000191"
     CDS             complement(53164..54168)
                     /gene="holB"
                     /locus_tag="OO315_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5889253.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(54165..54806)
                     /locus_tag="OO315_000192"
     CDS             complement(54165..54806)
                     /locus_tag="OO315_000192"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5889254.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54796..55818)
                     /gene="yceG"
                     /locus_tag="OO315_000193"
     CDS             complement(54796..55818)
                     /gene="yceG"
                     /locus_tag="OO315_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5889255.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55821..56630)
                     /gene="pabC"
                     /locus_tag="OO315_000194"
     CDS             complement(55821..56630)
                     /gene="pabC"
                     /locus_tag="OO315_000194"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5889256.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56750..57991)
                     /gene="fabF"
                     /locus_tag="OO315_000195"
     CDS             complement(56750..57991)
                     /gene="fabF"
                     /locus_tag="OO315_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5889257.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(58078..58314)
                     /gene="acpP"
                     /locus_tag="OO315_000196"
     CDS             complement(58078..58314)
                     /gene="acpP"
                     /locus_tag="OO315_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5889258.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58525..59259)
                     /gene="fabG"
                     /locus_tag="OO315_000197"
     CDS             complement(58525..59259)
                     /gene="fabG"
                     /locus_tag="OO315_000197"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5889259.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(59272..60201)
                     /gene="fabD"
                     /locus_tag="OO315_000198"
     CDS             complement(59272..60201)
                     /gene="fabD"
                     /locus_tag="OO315_000198"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5889260.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(60217..61170)
                     /gene="fabH"
                     /locus_tag="OO315_000199"
     CDS             complement(60217..61170)
                     /gene="fabH"
                     /locus_tag="OO315_000199"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5889261.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(61238..62308)
                     /gene="plsX"
                     /locus_tag="OO315_000200"
     CDS             complement(61238..62308)
                     /gene="plsX"
                     /locus_tag="OO315_000200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5889262.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62389..62562)
                     /gene="rpmF"
                     /locus_tag="OO315_000201"
     CDS             complement(62389..62562)
                     /gene="rpmF"
                     /locus_tag="OO315_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5889263.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62614..63135)
                     /gene="yceD"
                     /locus_tag="OO315_000202"
     CDS             complement(62614..63135)
                     /gene="yceD"
                     /locus_tag="OO315_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5889264.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            63334..63918
                     /gene="yceF"
                     /locus_tag="OO315_000203"
     CDS             63334..63918
                     /gene="yceF"
                     /locus_tag="OO315_000203"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5889265.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(64030..64989)
                     /gene="rluC"
                     /locus_tag="OO315_000204"
     CDS             complement(64030..64989)
                     /gene="rluC"
                     /locus_tag="OO315_000204"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5889266.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65562..68753
                     /gene="rne"
                     /locus_tag="OO315_000205"
     CDS             65562..68753
                     /gene="rne"
                     /locus_tag="OO315_000205"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5889267.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68950..69892)
                     /gene="flgL"
                     /locus_tag="OO315_000206"
                     /pseudo
     CDS             complement(68950..69892)
                     /gene="flgL"
                     /locus_tag="OO315_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69904..71546)
                     /gene="flgK"
                     /locus_tag="OO315_000207"
                     /pseudo
     CDS             complement(69904..71546)
                     /gene="flgK"
                     /locus_tag="OO315_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71612..72553)
                     /gene="flgJ"
                     /locus_tag="OO315_000208"
     CDS             complement(71612..72553)
                     /gene="flgJ"
                     /locus_tag="OO315_000208"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5889268.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72553..73650)
                     /gene="flgI"
                     /locus_tag="OO315_000209"
     CDS             complement(72553..73650)
                     /gene="flgI"
                     /locus_tag="OO315_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5889269.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTVSLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73662..74360)
                     /gene="flgH"
                     /locus_tag="OO315_000210"
     CDS             complement(73662..74360)
                     /gene="flgH"
                     /locus_tag="OO315_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5889270.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74413..75195)
                     /gene="flgG"
                     /locus_tag="OO315_000211"
     CDS             complement(74413..75195)
                     /gene="flgG"
                     /locus_tag="OO315_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5889271.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(75333..76084)
                     /gene="flgF"
                     /locus_tag="OO315_000212"
                     /pseudo
     CDS             complement(75333..76084)
                     /gene="flgF"
                     /locus_tag="OO315_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(76104..77312)
                     /gene="flgE"
                     /locus_tag="OO315_000213"
     CDS             complement(76104..77312)
                     /gene="flgE"
                     /locus_tag="OO315_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5889272.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATTQNGYKPGDLVSYQINDDGTVVGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQD
                     QILNTRVNLR"
     gene            complement(77337..78032)
                     /gene="flgD"
                     /locus_tag="OO315_000214"
     CDS             complement(77337..78032)
                     /gene="flgD"
                     /locus_tag="OO315_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5889273.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(78044..78447)
                     /gene="flgC"
                     /locus_tag="OO315_000215"
                     /pseudo
     CDS             complement(78044..78447)
                     /gene="flgC"
                     /locus_tag="OO315_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78451..78867)
                     /gene="flgB"
                     /locus_tag="OO315_000216"
     CDS             complement(78451..78867)
                     /gene="flgB"
                     /locus_tag="OO315_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5889274.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            79022..79681
                     /gene="flgA"
                     /locus_tag="OO315_000217"
     CDS             79022..79681
                     /gene="flgA"
                     /locus_tag="OO315_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5889275.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79757..80050
                     /gene="flgM"
                     /locus_tag="OO315_000218"
     CDS             79757..80050
                     /gene="flgM"
                     /locus_tag="OO315_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5889276.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            80055..80471
                     /gene="flgN"
                     /locus_tag="OO315_000219"
     CDS             80055..80471
                     /gene="flgN"
                     /locus_tag="OO315_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5889277.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80511..82046)
                     /gene="murJ"
                     /locus_tag="OO315_000220"
     CDS             complement(80511..82046)
                     /gene="murJ"
                     /locus_tag="OO315_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5889278.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(82246..83079)
                     /locus_tag="OO315_000221"
     CDS             complement(82246..83079)
                     /locus_tag="OO315_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5889279.1"
                     /translation="MKKLRIGVVGLGGIVQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(83081..83728)
                     /locus_tag="OO315_000222"
     CDS             complement(83081..83728)
                     /locus_tag="OO315_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5889280.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83739..84323)
                     /gene="rimJ"
                     /locus_tag="OO315_000223"
     CDS             complement(83739..84323)
                     /gene="rimJ"
                     /locus_tag="OO315_000223"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5889281.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84559..85767
                     /gene="mdtH"
                     /locus_tag="OO315_000224"
     CDS             84559..85767
                     /gene="mdtH"
                     /locus_tag="OO315_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5889282.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85831..86478
                     /gene="grxB"
                     /locus_tag="OO315_000225"
     CDS             85831..86478
                     /gene="grxB"
                     /locus_tag="OO315_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5889283.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86612..87172
                     /locus_tag="OO315_000226"
     CDS             86612..87172
                     /locus_tag="OO315_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5889284.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            87278..88324
                     /gene="pyrC"
                     /locus_tag="OO315_000227"
     CDS             87278..88324
                     /gene="pyrC"
                     /locus_tag="OO315_000227"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5889285.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            88398..88643
                     /gene="dinI"
                     /locus_tag="OO315_000228"
     CDS             88398..88643
                     /gene="dinI"
                     /locus_tag="OO315_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5889286.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88933..89187
                     /gene="bssS"
                     /locus_tag="OO315_000229"
     CDS             88933..89187
                     /gene="bssS"
                     /locus_tag="OO315_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5889287.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            89302..90420
                     /gene="solA"
                     /locus_tag="OO315_000230"
     CDS             89302..90420
                     /gene="solA"
                     /locus_tag="OO315_000230"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5889288.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90468..90581
                     /locus_tag="OO315_000231"
     CDS             90468..90581
                     /locus_tag="OO315_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5889289.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90842..91408
                     /locus_tag="OO315_000232"
     CDS             90842..91408
                     /locus_tag="OO315_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5889290.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            91412..91987
                     /locus_tag="OO315_000233"
     CDS             91412..91987
                     /locus_tag="OO315_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5889291.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(92029..93081)
                     /locus_tag="OO315_000234"
     CDS             complement(92029..93081)
                     /locus_tag="OO315_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5889292.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            93306..94226
                     /gene="lpxL"
                     /locus_tag="OO315_000235"
     CDS             93306..94226
                     /gene="lpxL"
                     /locus_tag="OO315_000235"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5889293.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            94398..94595
                     /locus_tag="OO315_000236"
     CDS             94398..94595
                     /locus_tag="OO315_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5889294.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94619..>95544
                     /locus_tag="OO315_000237"
     CDS             94619..>95544
                     /locus_tag="OO315_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5889295.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFRGIIKCEVY"
BASE COUNT        22989 a        25315 c        23576 g        23664 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tactgttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgtatgtgct gtatgggtgc
    15781 tcagtctctg atccctttca gaaattctat caatgcatct gtctgttcag gatttactgc
    15841 cagcatttca ccggatagcg cgcaacgaac aaaaccatga tgatcctttt caatcagcgc
    15901 ccccgtttcc agaaatgcgc ggtaatcagt gatattcatc gtctccatgt tgtcagcatg
    15961 gtatttatca cgctgtttaa gtatctcatc aaatttcatc ggcattgcgt tttcctctgt
    16021 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc agtagtgctc
    16081 cataccgttt caaatgacgc aaaaaaagcc ggattgctcc ggctgcgtgg tgtggttctg
    16141 atattcctac tctgctaatt tgctcagccc catgtctgcg taagtgcgat ttactgcatt
    16201 tctcaggtct gcgtagttct ccgttggcgg ctccggtgtg cccttgctgc tctgttttac
    16261 cagcgggtcg cccaccagct gcccgtaagc tgcaatttgt gctgtcatat tgccacctca
    16321 gtgaagcgat ccggcattgt tctttttcat ctcttccatc attttttcag tgagcattac
    16381 gcactgaaac cccatgtttt tgatgtctac gggtttcatg cctttgtcag tggatatcac
    16441 aggcagaata tcctgataca catcgcggtc agggtaacta agcacacaaa gctcccatga
    16501 tgccacgggt gaggatcctt cgtctgtttc aacccaccag cctggtgtag ctgcgcttat
    16561 ttgcagaatc gtgatccgtc cgttgtgctt gtaaaatttt tcaccaaatt ttggttcgct
    16621 catgattcca cctctccggt tttaacgttg attgttgtta cctgttccgc ttcggcaatc
    16681 tcccgctctg tcagcgtggc aaaatttgcc gccgtcgtgg tcatgaatgc gcttatcagt
    16741 tcgggatgtg ctttcgcgta tccttccccc gcgtggcggt ctatcgttct gattgccacc
    16801 tttaaagcgt gctctgtcat gtctaacgcg cgatatttcg gttctgttct gtctctgcgt
    16861 tttttgaatg atttattaaa tttccctgaa gtgtgcatat ttatttttac cccctcgttt
    16921 aaaaagtttt gagttgtgcc tccccttgtc taccttatct accttagtgg ccctcatgcc
    16981 agtaatggcg ctgctttcag cggggtagag tgcttttatc cactatctac cccgtgtcta
    17041 cctccctgtc tgattcaggt aaaatcaggt agagagggta gatagtgggt agacagtaaa
    17101 aaaaggctat ctacctaact taatgcactg aattaaatgt atttttcttt actcaggtag
    17161 acagggtaga cagcaattac aaaaaattat aaaaacgcgt cgcactcgtc tgttgttatt
    17221 gcgttagtct gcgttactcc cttaactttc cgcgtaatat attcatgtcc gtaaactttc
    17281 gcggctggct tcatagcctt gccaaagtca tttacgttta gcggtttgct cctgcctgcg
    17341 tacgccataa acgccagata gacgcggtaa aggctgtttc tggtcgtgta cttcactgaa
    17401 tcgccaccgc cgcccatcat caggccgcgt gcttcctcca gaaaattcag gaactggcaa
    17461 aactcaataa ccggatccgt ctgttgcttt attgccagag cttcatcacc gtcacgctgt
    17521 tccagtagta aagcccgtgc tttctcagag tcggtaaagt tcgccagcaa tcggcggata
    17581 atgacaggga tttcagccgc aatcttttcc ggtagctccc tgtctttttc ggcctcacta
    17641 acgatattgt cgaaacggaa aatcacgcga cgacgtgcca cacctccggc ccgttcggtg
    17701 aatatcatcg ggttattgtt ggtcgccagc accaccgccc tgattacagc cgtgaaacgc
    17761 ttttcgtatt tcgggttaat ttccacgggg tcaccgcccg tgattttctt gatgcccgtt
    17821 ccttcgcctg tatatttcgg ctggtcagcc aggacgataa gacgactccc gacaacctgc
    17881 gcacgtccac cagcatcatc aagcgatgtc atttcagcgc ttaccgtgtt ctgtttccct
    17941 gccagaaggc tggctatgtg tgtgaatgta cttttaccgc tcccgccgtc tccggtggcc
    18001 tcaataaaca tctgccagtc gtaccggttc gccataatca tgtacagcgc ggcacatata
    18061 cgcatcatct tgcgctggtc ttttccggct gcgtgctcaa gccatttatg aaagtttagc
    18121 gcgttatcgc ggatgttctc ccctggtgct ggtggcgtgt actcaatgcc gttgtgcgtg
    18181 gtgatccagt tctccggcgt gtgcggggaa aattcccccg ttttcaggtc aagcgcacca
    18241 ttggcgaacg gcagcaaatc gccggacggc tcgcccattg gttcggcaat aacttttaac
    18301 gcttccacgg cgttattgat tacgcgcttg ctgaaagtgg ccctgtgctc tgaatagatc
    18361 accaccattt cgcggctaag ttccattgtg ctgaccggac accatacccc gccgcgccat
    18421 acgtgaacga tttcactttc ctgatgcaca caaacgccat caaagcgctc ggcaagcagc
    18481 tgcgcgcgct cactgtctgc catttgtgcc agttgcgtat ttttctgctc cggcatggta
    18541 agccctgcgg caatattttc acgctcagca ttcagatagc gccgccagtt ttcacacttc
    18601 tggacgtgca ttccttcggg gtaaaaattt gcgtcctgaa tatctgccgc cgccagcttc
    18661 tgaccaatct ttttggtctc caccaaatcc agttctccgg cctggtacag ccttacgcgt
    18721 ttttttccat ccggaacaat tttcagtgca tcaagttcgg caagctgatt tggcccaagc
    18781 cacacaggcg gcacattatc gccggatgcg gggccatcct gctcctgcca ttgtttcgcg
    18841 tgcgaccagg catcactacc cgcaaagata attacctctg tgtctttgtg ttttattccg
    18901 cgtggctgtt tttttacgtt cggtgccagt ttcatttttt acccctgaat acgttaagca
    18961 tcttttttat ttcctgaata ttggcgcgtg ctttctccct gctggttggt ttactgcggg
    19021 gcgctgcctg taccagcgtg aaatcacgcc ggaattgata aacaggcatc acgcaatcat
    19081 attcgtaacc ttcacggcgg taggtgatgc gccgttctgc cacgccttta atcgttacca
    19141 tgccgccgta ctggtcgcgg taaatatcac cgcgcgtaaa tttagggtga gtgttgccac
    19201 tggcagttaa gccagaatat ttaagtttca ttatttttat tctccggtgt gttgttcttt
    19261 atatctgtcg tgcaatagat ctatttcttg cagttccatt attacaggct caagaagcgt
    19321 tattaatgcc gtggcaattc ttgatttttg tttgtcgcgt tcattgtcgc caagtgtttc
    19381 aagccatatg cgcaatattt ccagcatgtt ttcactgtga gaaagtgcaa gaaatgcgcg
    19441 gtctattgtt tcgtggtaaa tatcacgcat attaatcccc gtccgtcgtt tttcttaaaa
    19501 taacctctgt cacgaaatca gcataatcgg cggcgatatt cagtacatca agcccgttga
    19561 tttatattct ctcgtggaaa gtaagaaaaa agccgctcta ataagttctg gcattgacga
    19621 aagcgcatca gccgcatcat caggaacgcc ggaaaattcc tgtttcaggg aattaaaacg
    19681 atcatcacgc ataacccccc ccattttcac aatcggcaac aagaatattt ttagcgtcat
    19741 tcagtgaccg cgttgcgatg tatcggatac attccagggc gaactgtgtc cggtctttct
    19801 gttcttctcc ctgcgcaagc tctgccgtgc attcaatatc aataagcgcg tgcatcagcg
    19861 tagtgagtgc ggcggctgct gcgttcggtg tggttttatt gtgcataggt ttccccctga
    19921 cgaatacggg cggcgaatac agcaacacaa ccggacgggc aacggctacg cgcttcgcgt
    19981 tccgtccaag cggttacgtg gatgatttgg gattctccgg cactcagtgc cagaaaacgc
    20041 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
    20101 gctcctagtt tacgttgttg gttagaagcc ccgttactgc tcctacagtg cggggctttg
    20161 ctttttgtgt tgattgtgtg taatgtgtca ctacacataa atacattacc tgaggtgtaa
    20221 ttaacgtgtc aacacacaaa aacgaaaggc gtggtaatcc tccgttccag ttccgtttag
    20281 atcctgactt acgggaaaag atggaaaagg cacaacaaca ggacggcgat gattctcttg
    20341 ctgcctggat aaagcgaata atccgcaaag aattacagca acgcggcata gaacccaaag
    20401 gataaaaact atcagcgccg tggtgtgagg tactacggcg cattgcttta caggcgcaca
    20461 caatgaccaa cgaagaatca accgaaaaaa catcacctgc acgaaaaaga cgacgcagaa
    20521 agaaagagca tgaatcagaa cgattcgcgc cgtgttcttt tgcccttgag aagtacctta
    20581 aagaatactc atcaaaggag agaacttcgc agttatggca acgcactgaa ccagactgat
    20641 tgcattgccc accagcctga tagcggctat catccccgtg cttatgtttg ggatcacaca
    20701 cacaaacggc gcagcgggtt atctgttcag aaaggcggct ccatttcggg gccgcttttt
    20761 ttatgcctga aaaaccccaa ttttgtggtt ttccagtttc accagggcga acgaatcccc
    20821 gcccacgttc tggcgtatat tcaatcttca tggttatagc tctgtgttca gatgattgat
    20881 gtgtggcggc tgcgtgccgc cagcgtgatt aatgaactgc cttgcagcta tccttccagg
    20941 ccagaacctc ggataaagac cagccaacgg aacgaccgcc aagtttacga cgtgatggga
    21001 attgtccggc cttttccagg cggtagcggc atgagcggct aaggcctgtt agcttttcgc
    21061 attctttttc acgtataaac cgatcagtgc ttaacactat tgcccccttt cgtttcttaa
    21121 agagttattt cgtgttctat tgcgttggga tgtgtctgat tgtgtcagga tgattcagag
    21181 ttggcaaatg ttgaggccgt atggtttaca gaaagaggaa taatcaggat aaaatccttt
    21241 aaattcatgt taatacaaag gcataaaata ttgtttcatg ccttttttct cgctatttaa
    21301 agagtgattc gctagtgtat aaaaaatcag taacacatta aaaatcagct acttataaat
    21361 ctgtacactt tttcacctct tgttcgtagt tgttccgcat tgttgctcat tgttgcacgt
    21421 tgtatctgtt cgcatatcca gtatgcgcat actgaaaaaa cacgaaaaaa attattttct
    21481 tctggctact ggtagcgtgg ttacgttttc atgtgttccc gccagtatcc ctaaccgctc
    21541 cgtccacata tccagcgcat tgcgtttagc atccagataa cgggaatgat tataaactcg
    21601 ctgcattcct ggcatctggt ggcctgtaag ctgctccacg acgtgaggat ccacgcctaa
    21661 atcgttcagc atcgttgtaa aggtgcgccg gatgtcatgt agtgaccagt gagggtgatt
    21721 tagcctcctg tgcgctaatc tgccgtactg cgacacgctt gtttcctgtt tcacttcccc
    21781 cagcaataag cccgtgtgcc tgttctgctc caccagctgc gtgacgaacg gcagtattgc
    21841 ttccggtatg ggccggaata ttgccacctt cgttttgctg tgttctttcg gcacggtcca
    21901 gagcatttcg gtaaaatccc actcgctgat ctccgataac ctcagttcta ccgtccggca
    21961 tccgaacaca atcaggaggc ggattaacgc gatgtagtag ggggagaata tttttttgtc
    22021 cagtgcctgc aataattcgc ccagttcttt ggtgcttaag acacgctcgc ttatatccgg
    22081 ttttttccca acgtccgcca cactcatatc atcaagaacg ttgctgattg catagcgccg
    22141 cctccggcag aacttaagcg cctgtttgca cgtctgtagc aagaatccgg cagtaacagg
    22201 cgttcgcttt gccacctggt caaaacaggc cagccagtgc cgtagctcgc atttatccag
    22261 cggcatagca ccaatgtgct gtattacgtg attattaagg cgctttttca gggcggcata
    22321 atccacgcgg ttttcctttg cgtacgactc aagccagtag gtgagcgcat cgccaaccgt
    22381 taccggcttt aacgcttcct gtacggtgta attaagctca tgacgtggat ttttcccctc
    22441 tgccagccat gcgcgacact gggcggcttt ttccctggct gatttcaggc tcagatcagg
    22501 ataatttccc agcttaatgc gttccggtgg tgcccccctt cccgttccgg ccctgtaagt
    22561 gaaataccag gttaaaaggc cactggttga atgcctgacg ctcaggtttc caccgtcatt
    22621 aagaaaggct gttttttggg cgggtgtgcc gttgattttc ctcagctgtg tatcgctcag
    22681 tttgttaagt gctctgctca taattttgat tccggtcatc acaattaact acaccattaa
    22741 ctacaccgat cgttgcacaa agggctacaa cgtgaaacaa gctggaacaa ggaaaatcac
    22801 aaatctgttt ataatcaaaa acataatgaa caatctgaaa cattatgaaa cggcaaaaaa
    22861 cactaattga taaaatatgt ccatacttca cgcattacgt taagcatccg ttataatcgg
    22921 ttgcagatac cagcctgtgg atgcttaaca tggaatacca actcactctt aactggcccg
    22981 attttcttga acgtcactgg cagaaacgcc cggtggtgtt aaaacgcggc tttaataatt
    23041 ttattgaccc gatctctcca gacgagttgg cgggtctggc gatggaaagc gaagttgaca
    23101 gtcgactggt cagtcaccag gatggcaaat ggcaggtcag ccacggcccg ttcgaaagct
    23161 acgatcatct cggtgaaacc aactggtcat tactggtaca ggcagtaaac cactggcatg
    23221 agccgaccgc cgcgctgatg cgaccgttcc gtgaactacc ggactggcgt attgatgatc
    23281 tgatgatttc tttttctgta cctggcggcg gcgtcggccc gcatctcgat cagtacgacg
    23341 tgtttatcat tcagggtacc ggacgtcgtc gctggcgagt gggcgaaaag ctgcaaatga
    23401 aacagcactg cccacatccg gatctgttac aggtcgatcc gttcgaagcc atcatcgatg
    23461 aagagctgga gcctggtgat attctttata ttccgccagg attcccgcat gaaggctacg
    23521 cgctggaaaa tgcgatgaac tattccgtgg gttttcgcgc gccaaatacg cgggaattaa
    23581 ttagcggatt tgccgattat gtgctgcaac gtgaactggg cggcaactac tacagcgatc
    23641 cggatgttcc acctcgtgct catcctgcgg atgttctgcc gcaagagatg gataaactgc
    23701 gtgagatgat gctcgaattg atcaaccagc cggaacactt taagcaatgg tttggcgagt
    23761 ttatatccca gtcacgtcat gaactggata tcgcgccgcc ggaaccgcct tatcagccag
    23821 atgaaatcta cgatgcgctg aaacaaggtg atgtgctggt gcgcctgggt ggtctgcgcg
    23881 tattgcgcat tggcgacgac gtgtatgcca atggtgagaa gatcgattcc ccgcaccgtc
    23941 cggcactgga tgcactcgcc agcaacattg cgctgactgc ggagaatttt ggcgatgcgc
    24001 tggaagatcc gtcattcctc gcgatgctcg cggcgctggt caatagcggg tattggttct
    24061 tcgaagggta agtttggatt taagccggat gcagcatcac atgccgcatc ccttttcgct
    24121 tacttccgtt gcgccgttaa ctcggcaata cggacgatca cctgcaccgc tttttccata
    24181 ccttccagaa tcacaaactc atgcttacca tgatagttgt aaccgccagt gaacaggttc
    24241 gggcaaggta atcccataaa cgacaactgc gcgccgtcgg taccgccgcg gatcggtttc
    24301 agttccggtt caatatcgca gtcacgcatc gcctgctggg cgatatcgag aatatgcgga
    24361 tgctcaacca ctttctcgcg catattgtag taactgtctt caatcaccaa ttcaatgtag
    24421 caatcaggat gtaacccttt gcccactttt ttggcgatct ccatcatttt acgtttacgc
    24481 gcttcaaact gtttacggtc gaaatcacgg atgatgtagt gcatatcggc ccgttcaacg
    24541 gtgcctttca tgctcgccag gtgatagaaa ccttcatagc cttctgtcat ttccgggctt
    24601 tcatccgccg gaacttccgc atgaatacgt gccgccagcg acagcgcatt taccatcact
    24661 cctttcgccg tgcccggatg aacattgtta ccgacaattt ttatattgac cgatgcggcg
    24721 ttgaagtttt caaactccag ttcgcctacg ccaccaccgt caacagtgta agcccagcgg
    24781 gcatcgaagg catcaacatc aaaatgtttc gcccctttgc ccacttcttc atccggggta
    24841 aaggcgacgc gaatatcacc atgcggaatg tttttctgtt gcaataccgc cagcgcggtc
    24901 atgatttctg caatacctgc tttgtcatca gcacctaaca aggttttacc atcggtggta
    24961 atcaacgtct gacccagtag ctgatgcagc accgggaaca taaccggtga taaaacttca
    25021 tcgccgatac ccagcgcaat atcgccaccg cgatagtttt caacaatttg cggattaaca
    25081 tttttgccgc tgcaatccgg tgaggtatcc acatgagaaa taaagccaat cgccgggata
    25141 tcgccaggga cgttagccgg taacgtcgcc atcaaagtgc ccttctcact taaggtcaca
    25201 ttgataagcc ccatttcttc gagctgctct ttcagcagat gcaataactt ccattggcct
    25261 tccgtgctgg gaacctgtct cacccctgct tttgattggg tatccagaga cacgtagttc
    25321 aaaaatcgct caagtagttt atccatgtag tcaccctcac tttttgtgac aacattatta
    25381 agaagcaaga aaagacaaat attgcgtcag gtcactttta cccctgcaag cgggaatatt
    25441 tatcagcatt accttaatga atataaagct aagccagtaa ttcacaacaa agattggcga
    25501 ttcaagcggt ttgccgtaga attaccgccc tcattaggag tcaccaaggt ggttaaccac
    25561 aaaccccgca tcggtaagcc atccgttgcg tttacatggg acagagtaaa aaattgaata
    25621 aacaaccgag ttcgctttca ccgctggtgc aattggcggg aattcgcaaa tgctttgatg
    25681 gtaaagaggt cattccccag ctggatctga ctatcaacaa tggcgagttc ctcacgctgc
    25741 ttggcccttc tggctgcggt aaaacaaccg ttcttcgcct gattgcaggt ctggaaactg
    25801 ttgattccgg acgcatcatg ctggataacg aggacatcac ccacgttccg gcggaaaacc
    25861 gctatgtgaa cactgttttc caaagctatg cacttttccc ccacatgacc gtgttcgaaa
    25921 atgtggcctt tgggttgcgc atgcaaaaaa cccccgctgc tgaaattacg ccccgcgtga
    25981 tggaagccct gcggatggtg cagctggaaa ccttcgctca acgtaaaccg catcagctct
    26041 ctggtggtca acagcaacgc gtcgccattg ctcgagcggt ggttaacaag cctcgtctgt
    26101 tgttgctgga tgagtcgctc tcagcgctgg attacaaact gcgtaagcaa atgcagaacg
    26161 agctgaaagc gttacagcgt aagcttggca ttacattcgt tttcgtgact cacgaccagg
    26221 aagaagcact caccatgtcg gacaggattg tggtgatgcg cgatggtcgc attgagcaag
    26281 acggcacgcc gcgtgaaatc tacgaagagc cgaaaaacct gtttgttgcc gggttcattg
    26341 gcgaaattaa tatgtttaac gccacggtga tcgagcgact cgacgagcag cgcgtacgcg
    26401 ccaacgttga aggccgcgaa tgtaatatct acgttaactt cgccgttgaa ccggggcaaa
    26461 aactgcatgt tctgctgcgc ccggaagact tacgtgttga agagattaac gacgacaacc
    26521 acgctgaagg gctgattggt tacgttcgtg agcgtaacta caaaggcatg acgctggagt
    26581 cggttgttga actggaaaat ggcaagatgg tgatggtcag cgaattcttc aatgaagacg
    26641 atcctgactt tgaccactct ctcgaccaaa aaatggccat taattgggta gaaagctggg
    26701 aggtcgtact ggctgatgaa gaacacaagt aagttccaga atgtagtgat tgtcactatt
    26761 gtcggttggc ttgtgttgtt tgtctttctg cccaacctga tgatcattgg caccagcttt
    26821 ttgacccgcg acgacgccag tttcgtcaaa atggtcttta cactggataa ctacacgcgt
    26881 ctgctcgatc cgctctattt tgaagtgcta ttgcactcgc tgaatatggc gctgatcgcc
    26941 accctcgcct gcctggtgct gggctatcca tttgcctggt ttctggcgaa gctgccacac
    27001 aaggtgcgtc cgctgctgct gtttctgctg attgttccgt tctggaccaa ctcattgatt
    27061 cgtatctacg ggctgaaaat tttcctcagc accaaaggct atctcaacga gtttttgctc
    27121 tggctgggcg ttatcgacac accaatccgt atcatgttca cgcccagtgc ggtgattatc
    27181 ggtctggttt acattctgct gccgtttatg gtgatgccgc tgtactccag tatcgaaaaa
    27241 ctggataagc cgttgcttga ggcggcgcgc gatctcggtg ccagcaagtt acagactttt
    27301 atccgcatca ttattccact gacgatgccg gggattattg ccggatgtct gctggtgatg
    27361 ctgccagcga tgggcctgtt ctatgtgtcc gacctgatgg gcggtgcgaa aaacctgctg
    27421 atcggtaacg tcatcaaggt acagttcctc aatattcgtg actggccgtt tggtgcagcc
    27481 accagcatta cgctgactat cgtaatgggc ctgatgttgc tggtttactg gcgcgcttct
    27541 cgtttgctga ataagaaggt ggaactcgaa tgatcggtcg actgcttcgc ggcggtttta
    27601 tgaccgctat ctacgcgtac ctgtatatcc caatcattat tttgattgtg aactccttta
    27661 acagctcgcg ctttggcatc aactggcagg gttttaccac caaatggtat agcctgctga
    27721 tgaacaacga cagcctgtta caggcagcgc agcattcact aacaatggcg gtgttttcgg
    27781 cgacgtttgc tacgcttatc ggttcactga cggcagttgc gctgtaccgt tatcgctttc
    27841 gcggtaagcc gttcgttagc ggaatgctgt ttgtggtaat gatgtcacca gatatcgtga
    27901 tggcaatttc gctgctggtg ctgtttatgc tgctgggtat tcagcttggc ttctggtcgc
    27961 tgctgttctc gcatatcacc ttctgcctgc catttgtggt ggtgacggtg tattcgcgcc
    28021 tgaaaggttt tgacgtgcgg atgctggaag cggcgaaaga tctcggtgcc agcgaattta
    28081 ccattctgcg aaaaatcatt ctgccactgg caatgccagc ggtggcggcg ggctgggtgt
    28141 taagctttac cctgtcgatg gacgatgtgg tagtttcttc attcgtcacc ggaccaagtt
    28201 atgaaattct gccgttaaaa atttattcga tggtcaaagt cggcgtatcg ccggaagtta
    28261 acgcgctggc aaccatatta ctggtgctgt cgctggtgat ggttattgcc agccagctta
    28321 ttgctcgtga taaaacgaaa ggtaacacag gggacgttaa atgaaaaaat ggtcacgcca
    28381 cctgctcgcg gcgggtgctc tggcactggg catgagcgcc gctcacgccg atgacaacaa
    28441 cacgctgtat ttctacaact ggaccgagta cgtgccgcca ggactgcttg aacagttcac
    28501 caaagaaacc ggtattaagg ttatctattc gacttacgag tcgaacgaaa ccatgtacgc
    28561 caagctgaaa acgtacaaag acggtgccta tgacctggtg gttccttcaa cctattacgt
    28621 cgataaaatg cgtaaagagg ggatgatcca gaagatcgac aagtcgaagt taacgaactt
    28681 cagcaatctc gatccagaca tgctcaacaa gccttttgac ccgaataacg actactccat
    28741 tccgtatatc tggggtgcga cggcgattgg tgttaacggt gatgcggtgg atccgaaatc
    28801 tgtcaccagc tgggccgatc tgtggaagcc agagtacaaa ggcagcctgc tgttgaccga
    28861 cgatgcccgt gaagtgttcc agatggcgct gcgtaagctg ggctactccg gtaacaccac
    28921 cgatccgaaa gagattgaag ctgcatataa cgagctgaaa aaactgatgc caaacgtcgc
    28981 agcgtttaac tccgataacc cggctaaccc gtacatggaa ggcgaagtta acctcggcat
    29041 gatctggaac ggttctgctt tcgttgcacg ccaggcgggt acgcctattg acgtggtgtg
    29101 gccgaaagaa ggcggcatat tctggatgga cagcctggcg atcccggcaa atgccaaaaa
    29161 caaagaaggc gcgctgaaat tgatcaactt cctgctgcgc ccggatgtgg caaaacaggt
    29221 tgctgaaact atcggttatc caacgccaaa ccttgcggcg cgtaagctgt taagtccaga
    29281 agtggcaaac gataagacgc tctacccgga tgctgaaacc attaaaaatg gcgaatggca
    29341 gaatgacgtt ggcgcagcca gcagcattta tgaagagtat tatcagaagc tgaaagcagg
    29401 acgttaattc agcaagcggg cggtggtatt accgcccgtt ttttagttag caaggatgtg
    29461 gctaatgagt caggacagta aagttttatc ccgctctttc gtcggtattg tggtccgctt
    29521 gtaggcctga taagcgtagc gcatcaggca atgcttcctg ctttataatc cctccagcaa
    29581 cttttcaaca aattctggca ccacctggct tgccgggccg taatatttct cggcaaattc
    29641 attaccaacc tgactcggtt caagattcag ttcaacggtg tgagcaccat gtagtttggc
    29701 ttcgtgtaca aacccagccg ccggatagac atgcccggaa gtaccaatgg caataaagac
    29761 atccgccatc gacaacgcca tataaatttc atccatgccg agtggcattt cgccaaacca
    29821 aacgacgtgc gggcgcaagg gtgccgggaa ctggcaacaa tggcatttat cttctggcgt
    29881 aacgtctccg gtccagtcga gaacctgacc actttgcgaa caacgaactt tcagcaattc
    29941 cccatgcata tgaatgacat tggtattacc tgcgcgttca tgcaggttgt ctatattctg
    30001 cgttaccagc aaaaagcgat cgccgagagc gtcttgcagt ttagccagcg caagatgcgc
    30061 ggcgttaggc tgaatttctg gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg
    30121 caccagttca ggatcgcgat cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc
    30181 ttcccacagg ccatctgcgg cgcgaaaggt acgaatacct gattccgcag aaattcctgc
    30241 ccctgtcagt acgagtactc ttggtttttc cattgcttcc ggcaccactt tatctctgaa
    30301 aaaaatacgc tgacgcaaac gctcgcgcag gcgacgttta tttttacgaa aacgacttaa
    30361 ccgatgaccc cgacgcgaca gcatagcaac ctctgttgtt taatcggtta gatgtaggaa
    30421 ggccgcacca cgcattcctc ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg
    30481 agctacaggt aagagatgac aaggcaacct gtccgccagt tgcgttgtga ttgccgggaa
    30541 attcgataag ccaccaccaa tgacgaccag gtcagggtca acaatggtca ggatatttcc
    30601 cagagaaacc gctaataaat ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc
    30661 ttgatcataa agcgcaataa tttcgggagc ctgcaacggt tgatgataat agtgttgata
    30721 cagccacgca aaaccgcgac cagacagata attttcaatg cagccatgct gaccacagcc
    30781 gcagcggcgt aacgggaaat ccaaccccat catggttaac gcatcaaccg gcagacgcat
    30841 atggccaaac tcgccggtaa tgtagctttt accagtaatc ggtttgccgt tgaaaaccag
    30901 cccgccgcca acgccggtgc cgagaattaa ccccatcacc agtggatatt gcgtaaattc
    30961 atcatcccag gcttctgaaa gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg
    31021 atcaagacgt gcgctcaggt cggcacgcag cggtttgccg ctggcagcgg ggacattagc
    31081 ggcatacagc ttaccatctt ctgtttccgg cattcccgga ataccgatgc cgacagaacc
    31141 tttacagcca aaacgttggt cagcttcagc taccagctca cacactgcat ctaaaaatgc
    31201 gtcatagctg tcacgcggtg tcggcacccg cttttcccac tgcaactgcc gaccgctatc
    31261 aaacacgcca agcgcaattt ttgttccacc aatatcaaac ccgtaataca ttgccgctcc
    31321 ttgttttaat gtactgcctt tactggccgc taaggactcg cgcagggtca atattactgg
    31381 cgcgccgcgc cggataccaa cttgccaaaa gactcagcaa caatgctgtg accagtacgt
    31441 agaagacgtc cagccagtgc aattccgatg gcaggaagtc aataaaataa atatcgctgg
    31501 agaggaactg atgaccgatc agcttttcaa tccactcaat aatcggggta agttgcagtg
    31561 aaacaactac gccgataacc accccacaca ggctgccgaa cagccctgcc agcaatccat
    31621 accagacaaa gatggcgcga attaaaccat ctttcgcccc cagcgttctt aatactgcga
    31681 tatcgccact cttgtctttc accgccatca ctaaggtgga gacgatgttg aaacaggcca
    31741 cgccaatcac cagtaccatc gccagataca taatggcgcg gatcatctgg atatcgcgat
    31801 acatatagcc gtaagtacca atccaacttt taatataaac atagctgttg gtcacttcac
    31861 ccgcatcgcg taccagctta ttggcgttga aaacatccgt cattttaagg gcaatacctg
    31921 acacgctgga acccatatca agatattgtt gggcatccgc cagcgggatc atggcaaaac
    31981 tgtgatcgag ttgaccactc aactgcaaaa taccggcaat gtgcaaacgc acacgttttg
    32041 gctgcatcag tttatgctcg ggattcgagt tggggatcat gatcgacacc caatcgccct
    32101 gcttcacttt cagcgcatcc gccacgcctt tgccgatgat aatttgctgt tcgcccgctt
    32161 taaaattgcg ccacgcatcc ccctgaacaa acgagggtaa tgcgctcaga cgctgttcct
    32221 gttgtgggtt aacgcccttc acctggattg cgcgcagatt cgctccactt tccaccagac
    32281 cggtgaaatt gatatacggc gcagcggcgg caatacccgg cactttctgc acgttatcca
    32341 gtgcttcctg ccagttagtc catggctgat ttaccgcttc gatttcgcca tgcggcacca
    32401 ccgccagaat gcggttattc agttcgcgtt caaagccgtt catcgcgctt aagccgacga
    32461 tcaataccgc cacgccgagg gcaatgccaa tggtagaaat gacagagatt agcgacacca
    32521 tgccgccgcg tcgccgtccg cgactaaaac gcaggccaat caataacgat aaaggcatcg
    32581 ccattactcc gcccccatca ggctcagttc cgccgtcaga cgtccatcac gcatttccag
    32641 ttggcggctc atacgtttcg ccagttgcag gtcgtgagtg accaccagga aagcggtgcc
    32701 ctgcaagcga ttcaactccc caagcaactg gaagatgctg tcggcgttac gcgcatcgag
    32761 gttaccggta ggttcatccg ccagtaccag acgcggatta ttgaccagcg cacgggcaat
    32821 cgctacacgc tggcgttcgc cgccagaaag ttcagatggg cggtgattcg cacgatgctc
    32881 cagccccacc gcttttaaca tctccagtgc acagctgttg atttcagcgg gctttttctt
    32941 gccaatcagc agtggcatag ccacgttttc cagggcagta aaatccggca gcaggtggtg
    33001 aaactgataa ataaagccca gcttctggtt gcgcagttct gctttcgccg ctgaagacag
    33061 tttgctcatc ggctgaccgt taaagatcac atcgccggag gttggcgtat ccagcccgcc
    33121 taacaggtgc agcaaggtac ttttaccgga accagagcta ccgacaattg ccatcatttc
    33181 accttcgccc acgctgaaac tgacattgag cagcacatcg gtttgcacac tgccttcctg
    33241 atagcgtttg cacaggttgt cgcattgcaa caggatctta ttcataacgt aaagcctcag
    33301 cgggttgtgt ggcggcagcg cgccatgaag ggtaaagcgt agacagcagc gcgatagcca
    33361 tcgccaccag cgcaataaca atgacctgta aaggttcgat agccaccggc agcgccgcgc
    33421 catcaagcag gacgccgatt atcggcatca gattatttaa ctggctggca agcagtgcgc
    33481 caagcgccgc tccgaggatt gcaccgataa tcccggcgct agccccttgc accataaaga
    33541 ccatcatgat ttgtcgcgga gttaagcctt gcgtttgcag gatcgcgact tcgccctgct
    33601 tctccatcac catcagcccc agcgaggtaa taatgttaaa cgccgcaaca gcgacaatca
    33661 ggctcagcag taagcccatc atattttttt ccatgcgtac ggcctggaac agctcgcctt
    33721 tacggtcacg ccagtcctgc catttgctgc cttcaggcag tttttgctga cttaaagagt
    33781 caactctcag cggctcatcc agccacaaac gccagccggt aatattggct gccggataac
    33841 gcatcagacg cgaggcatcc tcaatattca ccagcatttc atagccatcg acttcactgt
    33901 tggcggcgaa agtgccaatc acattgaaca ggcgctgact tggaatacgc cccatcggcg
    33961 tgaactggct ggcagatggc accatcacgc ggatttgatc accgcgattg acgcccaact
    34021 gtgaggcaag ttgttcgccg aggatgacat tatatttccc cggctcgagg tcagtttgtt
    34081 tcacattgac cagatacggc gttagtggat ctttttgcgc cgggtcgata ccgagcatca
    34141 ccccgaccgc cacgctgcgc gcgctttgca gtaccacatc accggtagta ataggtgcga
    34201 cgcgattaac gccgtccagt ttgactgccg tttccgggag ttgctgcggg ttaagagagc
    34261 catgctcaga agagagaatt gcctgtggca tcaggccaag gatgttgttt tgcagctcgc
    34321 gctcaaagcc gttcatcact gacaatactg tgaccagcgc catcaccccg agggtaatgc
    34381 cgatggtaga aagccaggag acgaaacgac cgaagcgatc cactgcacgc ccacgcatgt
    34441 aacgcaggcc aataaataga gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc
    34501 aaagacacga gtatataagc gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt
    34561 cagggctaat ctgaaaagcc cccgtaaatc aatcggctaa attgccatat tttatcatca
    34621 atcttgccgc ctgatcttaa tctgaaaaat catccaatac agacagatac ccattacacc
    34681 tcgggtcata accagcagga tcaaccgcat gtaaattaaa aaagagcgtg ttgttctgat
    34741 gaaacaaaaa gagctatgga ttaaccagat caaagggtta tgtatttgtc tggtggtgat
    34801 ttatcactcg gtcattacct tttatccgca tatgaccact tttcagcatc cgttatcgga
    34861 agtcctgagc aaatgctgga tctatttcaa tctttacctt gccccctttc gtatgccggt
    34921 ttttttcttt atatccggct atttgattcg ccgctatatc gacagcgtac cgtggggaaa
    34981 ttgtctcgat aaacggatct ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca
    35041 gtggctggcg ttaagtgcgc tgaaccagtg gctggcacct gagcgcgatt taagcaatgc
    35101 ctccaatgcc gcttatgccg attccaccgg tgagttcctg cacgggatga tcaccgccag
    35161 caccagcttg tggtatctgt atgcgttaat tgtctatttc gtggtatgta aaatttttag
    35221 ccgtctggcg ctgccactat tcgccttgtt tgtactgctg agtgtggcgg ttaatttcgt
    35281 tcccacgccg tggtggggaa tgaacagtgt gatacgcaat ttgccttatt acagccttgg
    35341 cgcatggttt ggcgcaacaa taatgacctg tgttaaagag gtgccgttgc gccgccatct
    35401 gctgatggct tctttgctta ccgttctggc ggtcggtgcc tggttgttta ctatctcgct
    35461 gctgttgtcg ctggtatcga ttgtggtgat catgaagctg ttttatcagt acgagcaacg
    35521 attcggtatg cgctccacca gcctgctgaa tgtgattggc tccaacacca ttgctatcta
    35581 caccacccat cgcattctgg ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc
    35641 agcacgctgg tcgccgcaag tcgaactgac actcctgctg gtttatccct ttgtcagttt
    35701 gttcatctgt actgttgcgg gcttgctggt aagaaaactt tcacagcgtg cattcagcga
    35761 tctgttgttc tccccgcctt cactgcccgc tgccgtcagt tactctcgtt aaaccatcac
    35821 cccagccggg gcgattcaat ttgctaaacc atgtcgatta cggataatga acagcattgc
    35881 ttatcagaga tatgccccca tatgttgagg catatcctaa cgagaatctg acaaccgtta
    35941 tgcctgaaca atatcgttat acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg
    36001 agttaaccgg cgcagcctgt gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc
    36061 cggtggtact cattgcacca gatatgcaaa atgctctgcg tttgcatgat gaaatcagcc
    36121 agttcaccga tcagatggtg atgaatctgg cggactggga aactcttccc tacgacagtt
    36181 tttcgcctca tcaggacatt atctcctcgc gcctttccac cctttaccag ctaccgacga
    36241 tgcagcgtgg catactgatt gttccggtga atacgcttat gcagcgcgtt tgcccacaca
    36301 gttttctcca cggtcatgcg ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat
    36361 tacgaaccca actggacagc gccggttatc gccatgttga ccaggtgatg gagcacggcg
    36421 aatacgccac gcgcggcgcg ttgctggatc tcttcccgat ggggagtgag ctgccttatc
    36481 gtcttgattt ctttgatgat gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc
    36541 gcacgctgga ggaagtagaa gcgatcaatc tgctgcccgc gcacgaattt ccgaccgata
    36601 aagcggcaat tgaactgttc cgcagccagt ggcgcgatac cttcgaagtg aagcgcgatc
    36661 cggaacatat ttaccagcaa gtgagtaaag gcacattacc tgctgggatc gagtactggc
    36721 agccattgtt cttcagcgaa ccactgccac cgctgttcag ttatttccct gccaatacct
    36781 tgctcgtgaa tactggcgat ctggaaacca gtgccgaacg tttccaggct gacacgctgg
    36841 cgcgttttga gaatcgcggc gtcgatccga tgcgcccgct gttgccacca caatcgctct
    36901 ggctgcgggt ggacgagctc ttctcagagc tgaaaaactg gccccgagtg cagctaaaaa
    36961 ctgaacattt accgacaaaa gccgcgaatg ccaatttagg tttccagaaa ctgccagacc
    37021 tggccgttca ggcgcaacaa aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt
    37081 tcgacggtcc ggtggtgttc tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac
    37141 tgctcgcacg aattaaaatt gctccgcaac gcattatgcg tcttgatgaa gccagcgacc
    37201 gtgggcgtta tctgatgatt ggcgctgccg aacatggttt tgtcgataag gcgcgtaatc
    37261 tggcgctgat ctgcgaaagc gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt
    37321 ctcgccgcac catcaacccc gatacactga tccgtaacct tgcggagctg catattggtc
    37381 agccggtggt ccatctggag cacggcgtcg gtcgctatgc cggaatgacc acgctggaag
    37441 ctggcggcat tactggcgag tacctgatgc tcacctatgc caacgacgcc aaactgtatg
    37501 ttccggtgtc gtcactgcat ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc
    37561 cgctgcataa acttggcggc gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag
    37621 tgcgtgatgt ggcggcggaa ttgctggata tctacgcgca acgcgccgcc aaagagggct
    37681 tcgcgtttaa acacgatcgt gagcagtatc agttattctg cgacagcttc ccgtttgaaa
    37741 ccacgccgga tcaggcgcag gccattaatg cggtacttag cgacatgtgt cagccgctgg
    37801 caatggatcg tctggtgtgc ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg
    37861 ccgctttcct ggcagtagat aaccacaagc aggtagcggt gttggtgcct accacccttc
    37921 ttgcgcagca gcattacgac aacttccgcg accgtttcgc caactggccg gtacgtatcg
    37981 aaatgatctc ccgtttccgc agcgccaaag agcagacgca aatccttgcg gaagtggcgg
    38041 aagggaaaat cgatattctg atcggtacgc acaaactgct gcaaagtgac gtcaagttta
    38101 aagatttagg cctgctgatt gtcgatgaag aacaccgctt cggggtgcgt cataaagagc
    38161 gcattaaagc gatgcgcgcg aacgtggata ttctgacgct tactgcaacg ccgatcccac
    38221 gtacgctgaa tatggcaatg agcggaatgc gtgacctgtc gattatcgcc acgccgcccg
    38281 cccgtcgtct ggcagttaaa acctttgtcc gtgagtatga cagcctggtg gtccgggagg
    38341 cgatcctgcg tgaaattttg cgcggaggac aggtttatta tctctacaat gatgtggaaa
    38401 acattcagaa agccgccgaa cggctggcag aactggtgcc tgaagcacgg attgccatcg
    38461 gtcacgggca gatgcgcgag cgcgaactgg aacgggtgat gaatgatttc catcatcaac
    38521 gtttcaacgt gctggtttgt accaccatta tcgaaaccgg gatcgacatc ccgacagcca
    38581 acaccattat cattgaacgt gcggatcact tcggtctggc gcagctgcac cagttacgcg
    38641 gtcgcgtcgg acgttcacat catcaggcat atgcatggct gctgacgccg catccaaaag
    38701 cgatgactac cgatgcacaa aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg
    38761 caggttttgc gctggcaacg cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg
    38821 aagaacaaag cggctcaatg gaaaccatcg gtttctcgct gtatatggag ttgctggaaa
    38881 acgccgtcga tgcactgaaa gccggacgcg agccgtcgct ggaagatctc accagccagc
    38941 aaacagaagt cgagctgcgg atgccgtcgc tattgccaga tgatttcatc cctgacgtga
    39001 acacgcgtct gtcgttctac aaacgtattg ccagcgccaa aacggaaaac gaactggaag
    39061 agatcaaagt cgagcttatc gatcgcttcg gcctgctgcc agatccggcg cgtaccctgc
    39121 tggatattgc ccgtctgcgc cagcaagcgc agaaactggg gatcaggaag ctggaaggta
    39181 atgagaaagg cggggtgatc gaatttgccg agaagaatca cgttaatccg gcctggttga
    39241 ttggtttgct gcaaaaacag ccgcagcatt accgccttga tggtccgacg cgcctgaaat
    39301 ttattcagga tttgagtgag cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg
    39361 aactggaaga gaacgcgatc gcttaatgta aatcgggggc gtttctgcgc ccccatctgc
    39421 aacatttaca aattctttgc actttcctgc actatccgac actgtcacca tccataattc
    39481 aggcttatct gtttattaca ataaccttat atttattatg tatttttggt gatgatcaaa
    39541 acgcgttttt ctcgctggct aacgtttttt acgttcgccg ctgccgtggc gctggcgcta
    39601 ccggcaaaag ccaacacctg gccgctgccg ccagcgagca gtcgtctggt tggcgaaaac
    39661 aaatttcatg tggtggaaaa tgacggtggt tctctggaag ccatcgccaa aaaatataac
    39721 gtcggctttc tcgctctgtt acaagctaac cccggcgttg atccttacgt accgcgcgcg
    39781 ggcagcgtgt taacgatccc gttgcaaacc ctacttccag acgcgccgcg cgaaggcatt
    39841 gtgatcaaca ttgcggagct gcgtctctat tactacccgc cgggtaaaaa ttcggtaacc
    39901 gtgtatccaa taggtattgg tcagttaggt ggtgacacgc tgacaccgac aatggtgacc
    39961 accgtttcag acaaacgtgc aaacccaacc tggacgccaa cggcaaacat ccgcgcccgt
    40021 tataaagcac agggaattga gttgcctgcg gtagtgccgg ctggaccgga taacccaatg
    40081 ggccatcatg cgattcgtct ggcggcctat ggcggcgttt atttgcttca cggtacgaac
    40141 gccgatttcg gcattggcat gcgggtaagt tctggctgta ttcgtctgcg ggatgacgat
    40201 atcaaaacac tctttagcca ggtcacccca ggcaccaaag tgaatatcat caacactccg
    40261 ataaaagtct ctgccgaacc aaacggtgcg cgtctggttg aagtacatca gccgctgtca
    40321 gagaagattg atgacgatcc gcagctgctg ccaattacgc tgaatagcgc aatgcaatca
    40381 tttaaagatg cagcacaaac tgacgctgaa gtgatgcaac atgtgatgga tgtccgttcc
    40441 gggatgccgg tggatgtccg ccgtcatcaa gtgagcccac aaactctgta aatcctggat
    40501 aaaaaaaatc cccgcgacac ggcatgtcgc ggggaaaaaa tcagcagtag caggcattaa
    40561 tgagggttaa tgcttattta taaattactg ctgttccatg gagggtattc ggaccggtta
    40621 cagaagtaat acggaaagat tttgcgccca tctcatccgc tttttgcgcc agctgctctt
    40681 ccagcgatcc cagatttgtc cccgcgttag cactgatagt accgactttt tgttggcctt
    40741 ctggcgttga ctgaacttcg acagccgcaa agttggcaaa tgacatggag cttaaaatcg
    40801 ccgcagcgat gaggttttta cgtttttcat aatagtggcc ttatgcagat gaatgacgtc
    40861 gcaagaaatt acttaacgat cgataactaa atgatagatg tgatctggat cacatacaag
    40921 atatttttta taacaatcat taattaattt aaaaatcttt aaatttcagt caaatagaaa
    40981 gcaattattt ttatgggatc tggactggtg aacggaggtg attattttta taataacctt
    41041 tcattcacag tcaccaggta caacggcatc atggcaactg actcaacaca atgtgtaaaa
    41101 aaaagccgtg gccgcccaaa agtgttcgac agggatgccg cgcttgataa ggccatgaaa
    41161 ttgttctggc aacacggtta tgaagcgact tctcttgcgg acctcgtcga agcaaccgga
    41221 gccaaagcgc ccacgctata cgcggaattt accaacaaag aggggttatt tcgtgccgtt
    41281 ctcgaccgct atatcgatcg ttttgccgct aagcatgaag cacagctgtt ttgtgaagag
    41341 aaaagcgtgg agtctgcgct ggctgactat tttgctgcca tcgccaactg ctttaccagc
    41401 aaagacaccc cggcaggctg cttcatgatc aacaactgca ccaccctctc cccagattca
    41461 ggagatatag ccaatacgtt gaaatcacgc catgcgatgc aagagcgcac tttgcagcag
    41521 tttttatgtc aacgacaagc gcgcggggaa atcccgaccc actgtgacgt gacacatctg
    41581 gcagaattcc ttaattgtat tattcagggg atgtcgatca gcgcacgcga aggtgcatcg
    41641 ctggaaaaac tgatgcagat tgccggaacg actttgcgtt tatggcccga actggtgaaa
    41701 taagtcgcaa aaaaggcccc tcagcctgag cgaatgaggg gccaaattgc agagtacagc
    41761 cttgttatac tttgttattc aaaatcagtt gcccgttact atctagcggg atctgcgtac
    41821 ccggatcgcg gtccatgcgg attttgccct gctgatcgcc aatcttatag gtcacatcat
    41881 aaccgagcat tttttctgac ttgtcataca ccgttttaca acgctgttgc gtagtcgtgt
    41941 aagtatcgct ttcctggaga gagccctgga tctggttacc ggcatatcca ccacccagcg
    42001 cccccacaac agtggcgaca tctttaccgc gaccaccacc aaactgatgc cctatcacgc
    42061 caccagcaac agcgccgagc accgacccgg taatgcgatt ttcatcctgc accggtcgac
    42121 gatgggtcac tgtgacgttg cgacactcct gacgcggtgt tttaaccgtt tccttgattg
    42181 gggttgcaga aacaacctga gcgtattgcg ggccccgttc aaacacgttc agactggcca
    42241 ctgccgctac gcccaacgca gctgcgacac caatcccgat acccgccaac attgatttat
    42301 tcacgggaac acctccttct ttgcagttat gccaatcatg caagcgaagg agaaaagcag
    42361 ctatcagaca gaagatgaaa agcgcgggaa tgactggaaa tacttaagga ttcggaggag
    42421 tctgaaggaa tgcctgatgc gacgcttaac gcgtcttatc aggcctaccg ctcgtaccat
    42481 ttgtccaggg cggataaggc gtttatacca catccgccag tgtacgtcga ttaatgcaac
    42541 ttcaaacgcg gacggataac gcggttaata ctccccacca gcatcattaa tccggtttta
    42601 aagtaaccat gcagcgcaat ctgatgcatt cggtatagcg agatatatac aaagcgcgca
    42661 attcgtcctt caatcatcat tgagccgcgc gtcaggttac ccatcaggct accaacggtg
    42721 gagaagttcg acagcgatac cagcgaacca tgatctttat actgataatt tttcagcggc
    42781 ttaccgttca tctgcgccag aatgttgttc attgcgcaag tcgccatctg gtgtgcagcc
    42841 tgagcacgcg gcggaacaaa gcccccttcc ggacgcgggc atgacgcgca gtcgccaata
    42901 gcgtaaatgt ctggatcgcg agtggtttgc agcgtcggtt ccaccaccag ctggttgata
    42961 cggttagttt caagaccacc gatatctttc aggaagtctg gcgctttgat cccggctgcc
    43021 cataccatca gatcagcctc aatatattcg ccatctttag tgtgcaggcc gccttcatca
    43081 gcactggtga ccatggtttg cgtcagcacg cgaacgccaa gtttcgttag ctcgttgtgg
    43141 gccgcagcag agatacgtgg cggtaatgca ggcaaaatac gttctcccgc ttctaccagc
    43201 gttacgttca gggcttcgtt ggtcaggcct ttgtaaccgt agctgtgcag ttgcttgatc
    43261 gcgttgtgca attcagcgga gagttctaca cccgtcgcgc cgccgccgac aatcgcaatg
    43321 ttcactttgc catttgcgcc caggttggca gagtatttca ggaacagatt cagcatctcc
    43381 tggtggaagc gacgcgcctg gtgcgggtta tcgaggaaaa tgcagttctc tttgacgcct
    43441 ggcgtattga aatcgttaga ggtgctaccc agcgccatga ccagggtgtc ataggcgatt
    43501 ttacgttccg gaaccaacag ttcacctttc tcgtcgcgta gttctgcaat agtgattgtt
    43561 ttcgcttcac gatcaatatc aatgacggaa cccaactgga actggaaacc atgattgcgc
    43621 gcatgggcca gatagctcaa cgcatcgacg ccttcatcaa gcgagccagt cgccacttcg
    43681 tgcagcagtg gtttccacag gtggctgtgg ttacgatcga ccagcgtaat tttggctttt
    43741 ttcttacgtc ccagcttatg ccccagctgt gttgccattt ccagcccacc agcaccgccg
    43801 ccgacaatca caatcttttt caatggcgta gtcaacgtgc cccccttaaa tttattaacc
    43861 aattgttaat taatggttat taacatagct cttaattaac aacaggttac agcaatgaaa
    43921 atgttcttcg ctattgagaa taacatgaat ggtgcattgg tcataccaaa attgatgtta
    43981 atcaagtttt gttgcttaaa agataggcaa ctttaccggg ggagatcaaa aaatagagtt
    44041 tattgtttat tatttgagca aattcggaag ggtaatatag ggtgaagcgt atatcgaggg
    44101 cgtttggtcg tcctgataag atgcgcagca tcgcatcagg cattgatgct cgactgtcgg
    44161 atgcggcgtg gacgccttat ccgacttaca gaattcggag cgcatcaggt accggcaagg
    44221 ttacctgatg cgacgagggc atttacccga gggttttgaa cgctttaatg cgctgtaaat
    44281 gcggggagat attcttgaat ttgtgcgtct gctcttcgtc ccagacaatc tcgtaataat
    44341 gatgcaactc ttcagatgtc cgctggctgt taagtgcttc atcattacgc gacaaaataa
    44401 ccagacaacg atcgcgattc ttctcgcgga agttggtcac acacttagtg gcaatatcgg
    44461 catactcttc cgggcgatca atcttgcctt ccatgttctc ataagggaac aaattagggt
    44521 tgaagatcac ctggcggata tcgcaaagaa aaccaatccg ttccgcccag tatccgccca
    44581 ggccaacgcc gcaaattagc ggacgctcgt caacgttcag ttgcaacatt ttgtccactt
    44641 ctttaagcag atgctgcata tcatgtttcg gatgccgcgt actgtagctt atcaagcgta
    44701 catccgggtc aataaactgc aattgtaaga ctttctcgtg gttacccgga ctgttagagt
    44761 caaaaccgtg taaatagata atcatcgctt cctcacataa gccagggtta gtgacctact
    44821 ttctcttcct gccagcgttc atgtaactga ttcagacggg tgctgatcgc tttccagcga
    44881 gccgagtcca tcagttcctg tcgcgaaaat gaacctttat gatacaaacg tgtaacacgt
    44941 tctgccttga tcggtgacag attatctaac acgctgacgg cccctttacg attattgcag
    45001 accaggatca tatcgcaacc cgcatccagt gatgcctgcc cgcgttcggc ataactgccc
    45061 ataatcgcgg caccttccat cgataaatcg tcagagaaaa tcacgccgtc aaaccccagt
    45121 tcctgacgca aaacggtttt cagccagtag ggagaaccgc tcgccggacg cggatcaaca
    45181 tcactgtaga tcacatgcgc aggcataatg gcgtcgagtt tattttcgcg gattaaggaa
    45241 ctgaagaccg acatatcttt agcgcgaatc tccgcttgtg gacgtggatc gcacggtgtt
    45301 tctttgtgtg agtctgccgt tactgcaccg tgtcctggga agtgtttccc ggtcgttttc
    45361 attccggctt catgcatacc atcaataaac cggctggcaa ttgccagggc tttttgtgga
    45421 tcggcatgat aagaacgctc gccaatcgcc gcgctgatat gcccgacatc cagcactggc
    45481 gcaaagctga tatcaatatc catagcgatc atttcgctgg ccatcagcca acccgcctct
    45541 tgcgccagtt tgccgccctc ttccattccc aacagcgcag caaaggattg tgctgccggt
    45601 aagcgggtaa aaccttcgcg aaaacgctgc acgcgtccac cttcctgatc taccgccacc
    45661 accagatgat tgcgcgatgc tgcgcggatc tggcgcacca gttcacgtaa ctgggcagga
    45721 tcatgatagt tacgcgtaaa gagaatcagc cctcccacca gcggatgcgc cagtatttca
    45781 cgctcttccg cgtccagttc gtaaccttcg acatccaaca ttactggacc cacactgctc
    45841 tccttattgt tttattaata gctgccgcca ggtgtcatcg gccagcctga taaattgttg
    45901 atcgccggtt tgtcgccagc ggtactcaaa ccaccctcct ttgagcatca gcagccaggg
    45961 aaaccatcgc ctgacctgac gccataattg cgccggataa atcttcgcgc gagtggcata
    46021 gtcattgacc aattgccggt gctgttcagt attttccacc cacaccgccg ccagttccag
    46081 cgcgatatca ccatctccag catactccca gtcgatgagt tttaaccctg atgcgctatg
    46141 cactaaattt ccggcgtgga catccatatg caatggactt aagcgtaaag gtcgtggttc
    46201 ccgcgctttg cgcagacgtt ttaacattcg cagccaaccc acagtccgcc gcgccggatc
    46261 gctttgctgc cagtacagtt ccagtaaggg caacagcgtt attcgccagc caaaacgtgg
    46321 ttgttgatgt agataataca gcaagcctgc cagttcgttg gtatccggca aatacgtttt
    46381 taccgcaccg ggcagatagt cgactaccat ccagtcacgg agatataaat gcggcttcgg
    46441 tgcaatgctt gcgggtagtt gtgataaagc ccgatactgg cgcaagaacg cggactgcgg
    46501 cgcatcagga tcgtgcggct gacgcacaac aaaacgctgg ccctgatgtt caatgagaaa
    46561 actaccgcca ctaagcccac tattaaacgt cgtgacggga tgaaactgcg ggaaaaagcg
    46621 cgacagcaat tcgtcgcgcg tgatgggatt attgctgcga aacggcacct ttacctgacc
    46681 agataatttc gcccgtctgc accagcatca gctgcatttg tagggtcgga gcgttaacgt
    46741 tgccagaggc gcaggagtac agcacgtaat gagcgccgac attgcgggca atgcctatgg
    46801 ctttactacg ggtgcctaaa ctgtcctgcg gcgacaaacc taactgttgc ttagccatcg
    46861 acagttgctg ggcggaaacc agggtaaatt tcccgttatt agccagcgca tttcgcagcg
    46921 tttcggtcgc ttctgcggca ttcagcgaac cgttagtacg gttgttaacg ctatcaacca
    46981 gcaggacgct acccgcagtc accccgtcag ccccaagcat cttactgacc atcggctgca
    47041 ttgcgccatt ccagtcataa tggcgaatat gcggcgcagg cggtgccgtt cgatcttcgt
    47101 gctcaattgg gcctggctgc tgcgggatcg tcggcaccga gggcactgtg gggacaggct
    47161 gttgtggctc ggctggttgt tccggcgctg gtttcacttc ttctaccggt gcaggttcac
    47221 gttgccccac acacccggcg agaaacatcg ccagcgcggt aatcaaggcg tagcgactca
    47281 tttttgtcat caagattcac ccctttacaa atatagataa agtctgactt tgtgcgcccc
    47341 cagaaaattg gcgctgccgt acagcgttac cgccgaatgt gcgggaatgg taacgctgcg
    47401 tggcctttcc agaggatgca tctccagccc tctggcgtca taccagtaaa aacgataatg
    47461 aacggtaacg ggttcttgcc tttcgttata aatcgttgag gatgctgaag gttgaatatc
    47521 agacgtcgaa aggacgggct tttctgcact gatgcccgca gccaataaag atgactccat
    47581 caccagcgat tgctcatcat tcaccggaat ttccggatgt gaacgacagc ccaccagcag
    47641 taacaacacc agagacacca gcccaaagca tccttttctc atcgttacag acctttatgc
    47701 gccagcattg gtcccagcgg acggccaccc aacaagtgca tatggatgtg gtaaacctct
    47761 tgtccgccat ggcggttggt attcatgatc agacgatagc catcttcggc aataccttct
    47821 tgctcagcaa tttttgccgc tacggtgatc atgcgtccca gcgcctgctc atgctcagct
    47881 gacacgtcgt tcacagtcgg aatgagaata ttcggaatga tcagaatatg cgttggcgct
    47941 tgcggcgaaa tatcgcgaaa cgccgttacc agatcatcct ggtagacgat atcggagggg
    48001 atctcacgac gaataatttt gctgaatata gtttcttctg ccacgacatt ttcctttttc
    48061 aaatagccat gcgttgcgcc atgctacgcc gcgccacact gcgagtatag agtatggtcg
    48121 agttaaacct gctctttcaa tcttaactca cgatttctta agcaaaaaat cgttttgctg
    48181 ccgtatatat cgccattatt cccatttctg cctgcgaatg cgctgaaaag acattctctg
    48241 tttggtttac acgacaaata tgaatgcgta tatttctcat ttgcatttgc atttgcagca
    48301 aaattattcg cacactaaat ataaccgctc atcaccctcc tccggcatga gcctgacgac
    48361 aacataaacc aagagatttc aaatgctttc aacacaattt aacagggata atcaatatca
    48421 agtcatcacc aaaccgtcac tacttgccgg ttgcatagca ctggcactat taccttctgc
    48481 cgcttttgct gcaccagcca ctgaagaaac ggtgattgtt gagggttcag ccacagctcc
    48541 agatgatggc gaaaatgatt acagcgtaac gtctacctct gcgggtacca aaatgcagat
    48601 gactcaacat gatattcctc agtcggtcac tattgttagc cagcagcgga tggaagatca
    48661 gcagttacaa acgctgggcg aagtgatgga aaacacgctg gggatcagca aaagtcaggc
    48721 ggattccgat cgtgctcttt attattcccg cggattccag atcgataact atatggttga
    48781 tggtatcccc acctattttg aatcgcgctg gaatctgggc gacgcacttt ctgatatggc
    48841 actgtttgaa cgcgtagaag tagtgcgtgg cgcgacagga ctcatgaccg ggacgggtaa
    48901 tccatctgcg gcaattaata tggttcgaaa acacgcgacc tgtcgtgaat ttaaaggcga
    48961 tgtctcggcg gaatacggta gctggaacaa agaacggtat gtggcggatt tacaaagccc
    49021 actcaccgaa gacggtaaaa tccgcgcgcg aattgtcggc ggctaccaga ataacgactc
    49081 atggctggac cgctacaata gtgaaaagac cttcttctcg ggcattgtcg atgctgattt
    49141 aggcgatctt acgacgcttt cagccggtta cgaatatcag cgcattgatg ttaatagccc
    49201 aacctggggc ggtttaccgc gctggaatac tgatggcagc agcaacagtt acgatcgcgc
    49261 acgcagtacc gcacctgact gggcgtacaa cgataaagag atcaacaagg tctttatgac
    49321 cctgaagcag cggtttgctg atacctggca agcgacactg aatgccaccc actctgaagt
    49381 cgaatttgac agcaaaatga tgtatgtcga tgcctatgta aacaaagcgg atggtatgct
    49441 ggttgggcca tacagtaatt atggacctgg ctttgatcat gtcggcggca ccggttggaa
    49501 cagtggcaaa cgtaaagttg atgcgctgga tttgttcgct gacggtagtt atgaattgtt
    49561 tggtcgtcag cacaatctaa tgtttggtgg cagttacagc aaacaaaaca atcgttcagt
    49621 tcatgggcca acatcttccc ggatgaaatt ggcagtttct acaactttaa tggcaatttc
    49681 ccacaagccg actggtcatc acagagcctg gcgcaggacg ataccacaca tatgaaatcg
    49741 ttatatgctg ccactcgtgt cacccttgcc gatccgctgc atctgatcct cggcgcacgt
    49801 tataccaact ggcgggttga tacgctgact tacagcatgg agaaaaacca caccacgcct
    49861 tacgctggtc tggtgtttga catcaatgac aactggtcga cctacgccag ctatacctct
    49921 attttccagc cgcaaaatga tcgtgacagt tcaggcaaat atctggctcc aatcaccggt
    49981 aacaactacg agctgggtct gaagtcggac tggatgaata gccgtctgac caccacgtta
    50041 gccatcttcc gtattgagca ggataatgtc gctcagtcca ccggtacacc tatccccggc
    50101 agcaacggcg aaaccgccta taaagcggtg gatgggacag tcagtaaagg ggtggaattt
    50161 gaactcaacg gcgcaattac cgacaactgg cagctgacat ttggcgcaac gcgctatctt
    50221 gcagaggata acgaaggaaa cgccgttaat cctaatctgc cacgcaccac ggttaaaatg
    50281 ttcaccagct atcggttgcc tgtcatgcca gagttgacag tcggcggtgg tgttaactgg
    50341 caaaatcgcg tgtataccga caccgtgaca ccgtatggca ccttccgcgc cgagcaaggt
    50401 agctacgcgc tggtggatct cttcacccgc taccaggtga cgaaaaactt ctcgttacag
    50461 gggaacgtca ataacctgtt cgacaaaacc tacgatacca acatggaagg ttctatcgtc
    50521 tacggcgcac cgcgtaattt cagcattacc ggcacgtatc aattctgaat aacacctgta
    50581 aaaaaggcag ccatctggct gccttagtct ccccaacgtc ttacggatta gtggttacgg
    50641 atgtactcat ccatctcggt tttcaggtta tcggatttag taccgaaaat agcctgaaca
    50701 ccagaaccag caaccactac gcccgctgcg cccagtttct tcaggccagc ctgatccact
    50761 ttagacacat cagcaacgct gacgcgcaga cgggtaatac atgcgtcgag gttagtaatg
    50821 ttttctttac caccaaatgc agcaaccaga gccggtgcca tttcgctggt acctgttgct
    50881 tttgcatctt cagtcgcgtc ttcacgaccc ggcgttttca gatccagtgc tttaatcagc
    50941 acgcggaaga tggtgtagta aacaatcgca taaccgatac cgacgatcgg taacagccac
    51001 agtttgctgc tgttaccaga cagaacgatg aagtcgatca gaccgtgcga gaacgacgta
    51061 ccgtcacgca tccccagaag aatacagatt gggaatgcca ggcctgccag aatcgcgtgg
    51121 atgatgtaca ggatcggcgc aacgaacatg aagaagaact cgatcggctc ggtgataccg
    51181 gtcaggaacg aggtcagcgc cgcggagatc ataataccgc ccactttcgc gcggttttct
    51241 ggtttagcag agtgccagat agcaattgcg gcagctggca gaccgtacat tttgaacagg
    51301 aagccaccag acagtttacc cgcagtcggg tcacccgcca tataacgcgg aatgtcgccg
    51361 tggaaaacct gacctgctgc gttggtgtat tcaccaatct gcatctggaa aggtacgttc
    51421 cagatgtggt gcagaccaaa cggtaccagg caacgttcga tgaaaccgta aatgccaaac
    51481 gcaactaccg ggttctggta agcagcccac tgagagaagg tctggattgc agaaccaatc
    51541 ggcggccaaa tgaaggacag cacaacgcca gtaaagatgg cagccaggcc agaaatgatc
    51601 ggcacaaagc gtttaccggc aaagaagcca agatactcag gcagcttaat acggtagaaa
    51661 cggttaaaca tgtacgctgc aatcgcaccg gagataatcc ctccgagtac gccagtatcc
    51721 gccaagtgtt tagaggcgat ttcttcagca ggtaaatgca gtaccagtgg cgcaaccacg
    51781 gccatggttt taaccatgat gccataggca acaactgcgg ccagcgcgga tacgccatcg
    51841 ttattggtaa agccgagggc gacaccgatc gcaaaaatca gtggcatgtt tgcaaagacg
    51901 gaaccgcctg cttctgccat aacatgcgat acaacggcgg gcagccagct gaaattcgcg
    51961 gaaccgacgc ccagcagaat acctgcgata ggtagtacgg ataccggcag catcagcgat
    52021 ttaccgacct tttgcaggtt agcaaatgca ttcttaaaca taattgagag tgctcctgag
    52081 tatgggtgct ttttttacgt tctcacgcgt ggcaaggggg gagagcctcg ccgtgtacag
    52141 ggcatctaag cgccctttat ttattacaca gagtaaaata attcagtgcc aatttgtttg
    52201 acggctatca cgtttcaact tcagatcatt ggagaagttg cacattttga caaaacatat
    52261 gtcacaatat ttcaaacgcg gttattcacc gcccaatttt cggcggtgaa ctttactcgt
    52321 tttagccatt aattacgagc tttaaaaaaa ctcattcaac ggatggattg aaggcgggaa
    52381 gcgtcgatgt gaaacagacg ggcgaagtta tcggtggtta cctgcgccag ttcttcaacg
    52441 gcaacacctt tcaacacagc catgtattct gcaacgtcac gaaccatcgc aggttgattc
    52501 tcttttcctc gatgcggtac cggcgcaagg taaggtgagt cagtttccac cagtaatcga
    52561 tccaggggga cataacgcgc agcatcgcgc agttgctccg cattacggaa ggtcacaatg
    52621 ccggaaaagg agatgtaaaa tccgagatcc agtaatttac ccgccgtttc tctgtcctct
    52681 gtaaaacagt gtagtacgcc accgcaatcc gtcacttttt cttcgcgcag aattgccagc
    52741 gtatcggcac gggcgtcacg ggtatggacg ataaccggct tgttcagttc acgaccaatc
    52801 tggatatgat ggatgaagga ctcttgctga cgtactttag tttccggtgt gtaataataa
    52861 tccagcccgg tttcacccag cgctacaaca ccctcttctg ccgccagacg gcgtaaatct
    52921 tctacatcgt agggatcatt ctggttgagt ggatgcacgc cacaagaaaa tacgacgttg
    52981 tcacgttcgc ctaccagatc ccgcatatgt aaataacccg gtaatgttgt ggcgactgcc
    53041 agacaaaatt tcacatcgcg tgcggcggct ttcgccagaa cgtcatccac gtccttatgc
    53101 aaagattcat aatccagacc atcgagatgg cagtgtgagt cgactaaaaa catgatgtct
    53161 ctcttaaaga tgaggaaccg gtaacacaac gcccggttgc aggtaatgct caatacgcag
    53221 taaaagatcg gtgatgagaa gctcgcggtt gatgcctgtg acagacatta actgttcacg
    53281 aatgtggcaa acatccccca gtatagcctg caggcacgag ggagaaagat ggtttgccag
    53341 ttcggcgacc aggcccggca catcaacatt ggtcacctgc gcagcgccat gatggcgttt
    53401 tagcgcatcc atcagcaacg ttgccagcca gtgtaaacgc gccggagctt gttcatgatt
    53461 aagggccgcc agcagcgaat accagtcgcc cgatggcacg ctatatgcca acgcctgaca
    53521 caatgtttca cgagcctgcc agttatctcc ctgaaacaac gccagtgccg cgccaggcga
    53581 accggcgctt aagcgcaatg cggcaagtaa tgcatcctgt gacattgtca cttcgcgtga
    53641 aagccaagtc acggcgtact gttctggagg cggcgcaagg taatgtaacc gacaacgact
    53701 acgtaatgtt gccagtaaac gttcaggctc gcgggtagcc aggaaaaacc aggtttctgc
    53761 tggtggctct tcaagcgttt tcagcaatgc gttagccgcg gcgtcggtca gtaaggcagc
    53821 atcggttacc cagacaactt tcgcaccacc taggcgtgcg tgctcattca gcttttcggt
    53881 gacctcacgt accgcatcaa tgcccagcgt atttttccct ttttcgggag ccagggtgta
    53941 gtaatcggga tgcgtgccag cctgcatcaa ctgacatcca cgacagtgac cgcaactttt
    54001 gtggccctgc ggttgttggc agagtaaata acggctcagg gcgtagatta aagcatcatc
    54061 gcccatgccc ggtaacgcct gaatgagtag cgcatggtga cctcttccgg cctgatagct
    54121 ggctaccagt ttttcgaaat caggtcgtaa ccatggatac catctcatgc gtccaactcc
    54181 ttcacccagt gggtcacggt agtgcggatt gcatccatca cggcctccag cggctgggtg
    54241 gcatcaatgg tatgaatact tttatcttgt gcagccagtt ccagatagcg ggcgcgggtg
    54301 cgattaaaga aatcgaaaga ctcttgctca atacgatcca gctcgccgcg cgcacgcgcg
    54361 cgttttaagc caacttccgg ggtaacatcg agatagagcg ttaagtctgg gcgaaaatcc
    54421 ccgagaacag catcacgcag tgttgccagc atatgttggt caataccacg tcctccgccc
    54481 tgatacgcct gagtggaaag atcgtggcga tcgccaatca cccaggtgcc gttagccagc
    54541 gcaggtttga tgaccgtttc taccagttga acgcgcgcgg cataaaacat cagaacttcg
    54601 gctttatcgg taatgacttc atcgcctacc gatttgatat ccagcaccag gcttcttaac
    54661 ttttcggcaa gttgcgtacc gccaggttcc cgagtgaaaa ccatgtcgcg gatacccagt
    54721 tgctcgagcg tctcaaccac cacattacgc gcggtagttt tgcctgcgcc ttccagcccc
    54781 tcaatgacga tatacttact gcgcattttt ttccttaagc actttcagat aatcctgcac
    54841 agacttgtta tgactggcaa gattggtatt aaacgtgtga ccacctttac catcggccac
    54901 aaaatagaga tacggcgttt ttgccggatg cgcagcagcc ttcagcgaat ccgcccccgg
    54961 cgtagctatc gcacccggcg gcaaaccggt aatggtatag gtgttatacg ctgtcggcgt
    55021 ttccaggtct gcacgagaaa gtttgccatt ataacgctct cccatcccgt aaatcacggt
    55081 tgggtcggtc tgcaggcgca taccaatgcg taaacggttg ataaataccg aggcaacctg
    55141 atcgcgttca ctggcaacgg cggtttcttt ttcgataatt gatgccatcg tcaccagctg
    55201 atttttatct ttataaggca gaccgtccgc acgcccttcc caggcgctat cgaccgcttt
    55261 caccattttc ttgtgaactc gcttgagtaa cgcgacatcg gtggtattgg cggtatacat
    55321 caaggtgtct ggccagaacc aaccttcaat ccactccggg ttttccagtt caagtgcctg
    55381 cgctacggtg gcgtacttat cgtcactcag cgtgtgcttg atatacggag cctcacgcaa
    55441 ttgcttgagg tagtcgctca gacgcatccc ttctaccagt cgcagcggga actgtgcttc
    55501 tttaccgctt tccaacaatt tcagcatctc gcgcacggtc atctgcggtg taaagcggta
    55561 agtcccggct ttaaagtgag aaagatccgg ttcgatacgc agcagccatt gaaaaacccg
    55621 tgggcgattg atgatcttat cagcataaag ctgttcaccg agcgccagac gtccggtccc
    55681 tggcttcagg gtaaatatcg tctcttcttt gataagcaat ttgctgtcgg caagatggcg
    55741 aaccttccag acgcccacac cagcggcgat acccagtacc accagcaata acaagataat
    55801 caataacact tttttcatga ctaattcggg cgctcacaaa gtggggctaa atattcatat
    55861 aacgttgctg acgaaaaggt gacatcgcca caggcacgta cgggcatcac tggcattaac
    55921 gcattacaaa taaccatctc atctgcctgc aacgcctcct ccagcgaggc ttgcacttcg
    55981 acaagctgat aagaggattg tgccagtaaa cggatacaga attgtcgcat aatgccgtta
    56041 acacctgcct gatccagtcg cggcgtataa actacattgc ccttccgcca gaacaaatta
    56101 gccgcacagc attccgtaac ccacccttcg ctgtcaagga ccagcgcctc atcagcgttt
    56161 gtctgctcaa gatgagagcg aatcaatact tgctcaagcc gattaagatg tttaatacct
    56221 gcaagatgag gattgcgccc cagccgcacc gggcttagcg ccaacgtcat cccttcgtta
    56281 cgcaaacggt cgtaatgtgc aggataagcc gtaacggaga gaatccgcgt tgctggtccg
    56341 ctgttcaatg tgctgtaccc tcgcccgcca ctaccgcgac tgatcacgac tttcagcaca
    56401 ccattttgct gttctgctgc cagcgttttc atctcttgtt caagctgagg ccagaagtca
    56461 caggaaatca tcaaccgctg acaagcatcc tgtagtcgca ggatatgcgc cgataacaaa
    56521 ctgactttac cgtcgataac tctggcggtg gtaaaacaac catcaccaaa ctgcgttgcc
    56581 cgatcgctta ctgccagcga ttcctgctta taaccgttaa ttaagaacat accggctcct
    56641 tatcagagaa ttgttagtgt ggcagcatgt tcactacgga acaagtcgga ataaaagcta
    56701 agaaaaaagg cccgcaagcg gaccttttat atgggtggaa aatgacaact tagatctttt
    56761 taaagatcaa agaaccatta gtgccaccga agccgaagga gttacacagc gtgtattcca
    56821 ttccgctgac ctgacgtgct tcatgcggta cgaaatccag atcgcaacct tcatccgggt
    56881 tatccaggtt gatggttggc ggaacagcct gatcgcgcag cgccaggatg gagtagatag
    56941 attctactgc acccgccgca cctaacaggt gaccggtcat agatttcgtg gagcttacca
    57001 acacacggct tgcagcttca ccgaagatgg ttttcaccgc ctgcgcttca gctttatcgc
    57061 cagccggcgt agaagtaccg tgcgcgttaa cgtagccaat ctgactcgct tcaatgcctg
    57121 catcacgcag agcatttgcc attgccagag ctgcgcctgc gccattttct ggcggtgacg
    57181 tcatatgata agcatcgctg ctcataccaa agccgacgag ttcagcgtaa attttcgcac
    57241 cgcgtttttt cgcgtgttcg tactcttcaa gtaccagcat accggcacca tcgcccagta
    57301 cgaagccatc acgctcttta tcccacgggc ggctcgccgc ttgcgggtta tcattgcggg
    57361 tagataatgc acgtgccgcg ccaaaaccac caacgcccag cggcgtactg gctttctctg
    57421 cgccacctgc aaccatcacg tcagcatcgc catacgcgat aatacgcgca gcatggccaa
    57481 tgttgtgcac gccggaagta caggcggtcg cgatagagat gcttgggcca cgcaggccat
    57541 acatgatagt cagatgacct gccaccatgt tcacaatcgt tgacggaacg aagaatgggc
    57601 tgatcttacg tggaccaccg ttcatcagag atgtgtggtt ttcttcgatc agtccgaggc
    57661 cgccaatccc ggagccaatt gcggcaccaa tgcgggttgc gttctcttcc gttatttcaa
    57721 ggccagaatc ctgcatggcc tgaacgccag cgacaattcc atattgaatg aaggcatcca
    57781 tcttgcgctg ttctttgcgc gagataatgt cctcacagtt aaaatccttt actaagccag
    57841 caaatttcgt tgcataggcg ctagtatcga aatggtcgat taggctgatg ccactctgac
    57901 cggcaagcag agctttccag gtagactcta cggtattgcc gacaggagac aacatgccca
    57961 gtccggtcac aactacacga cgcttagaca cgtttgtcct ccaaggaggg aaaaatgatt
    58021 ctagtgggac aaaaagataa aactcaggcg gtcgaacgac cgcctggaga tgttcactta
    58081 cgcctggtgg ccgttgatgt aatcaatggc agcctgaacg gtggtgattt tctcagcttc
    58141 ttcgtccgga atctcagtat caaactcttc ttccagagcc attaccagct caacggtgtc
    58201 aagagaatcc gcgcccaggt cttcaacgaa agaagcattg ttggtaactt cttcctgctt
    58261 aacgcccagc tgttcgccga taattttctt aacgcgttct tcgatagtgc tcatactctt
    58321 aaatttccta tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa
    58381 tttgcaacta aatcccggca ggtcttacca cgattttacg ttattttgag gccttacccc
    58441 ccaataacgc aaatcatttt gcactaatta ttactcataa ccacgcagac tgcgcgcaac
    58501 atgagcattt tgtgcaaatc gcggtcagac catgtacatc ccgccgttca catgcaaagt
    58561 ttcacccgtg atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc
    58621 ctgtgcgccg ccgaggcgac ccgcaggaac ctgcgccagg atacccgcac gctggtcatc
    58681 gctcagcgca cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat
    58741 accgcgtgac gcaacttcgc gcgccagtga tttactgaag ccgatcaagc ccgctttcgc
    58801 cgcagcgtag ttggcctgac cgccatttcc catggtacca accacagaac cgatagtgat
    58861 aatacgacca tgacgctttt tcatcatagc gcgcattacc gcttttgaca gacggaaaac
    58921 agatgaaagg ttagtttcga taatatcgtt ccactcttca tctttcattc gcattaacag
    58981 gttatcacga gtgataccgg cattattgac caggatatcc acttcaccaa attctgcgcg
    59041 aattttttcc agaacagatt cgatagatgc cgggtcggtc acattcaaca tcagaccttt
    59101 gccgttggca cctaaataat cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc
    59161 aataactttc gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct
    59221 tgcaccggtt accagtgcga tttttccttc aaaattcatg attttcctct tttaaagctc
    59281 gagcgccgct gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat
    59341 gcgtttcgtc aggccagtaa gcaccttgcc cgggccgact tcatagagat gttctacgcc
    59401 ttgcgctgcc atgtactcaa cagacttcgt ccactgaacc gggttataca actgacgtac
    59461 cagtgcgtca cggatggcat caccattggt ttcgcatttc acatcaacgt tattcacaac
    59521 agaaactgtt ggtgcgttaa aggtgatttt cgctaattct actgccagtt tgtcggctgc
    59581 tggtttcatc agcgcacagt gagacggtac gctcactggt aacggcagcg cgcgttttgc
    59641 gccagcagct ttacaggcag cgccagcacg ctcaaccgct tctttatgac cggcaataac
    59701 cacctgtccc ggagagttaa agtttaccgg agaaacgacc tgaccttctg cagcttcttc
    59761 acacgctttc gcaataggcg catcatccag accgatgatt gccgccatag cgcccgtgcc
    59821 ttccggtacg gcttcttgca tgaacttacc gcgcatctca accagacgca ccgcatcagc
    59881 gaaatcaatc acgccagcgc aaaccagcgc ggagtattcc cccaggctgt gaccggccat
    59941 cattgccggt gctttaccgc cctgctgctg ccatacgcga tacagcgcaa cagatgcagt
    60001 caacagcgct ggctgggttt gccaggtttt attcagttct tcagctgacc cctgctgggt
    60061 cagcgcccac aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt cttcgacaat
    60121 tggatagctc gccgccatat cagccagcat tccaacggtt tgagaaccct gtccagggaa
    60181 cacaaatgca aattgcgtca tgttttaatc cttatcctag aaacgaacca gcgcagagcc
    60241 ccaagtgaat ccaccgccaa aggcttcaag cagaaccaac tgccccggct taatgcgccc
    60301 gtcgcgtaca gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg
    60361 atccagcgtc accacgacat tgtccatcga cataccgagt tttttcgccg ttgcactgat
    60421 aatacgcagg ttagcctgat gcggaaccag ccagtccagt tgagaacggt caagattatt
    60481 cgccgccagc gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct tgaagacttc
    60541 gttgcccgcc atcgtcagat gaattgaatt ctctggattc acgcggtcgg cgtttggcag
    60601 cgtcagcaat tcaccataac taccgtcggc atgcagatgg gtggaaatga ttcccggctc
    60661 ttcagaggca gccagcaccg cagcgcccgc gccatcgcca aaaataataa tagtcccacg
    60721 atctgttgga tcgcaggtgc gcgccagtac atcggaaccg acgaccagag catacttcac
    60781 cgccccagat ttcacgtatt gatcggctac gcttaatgca taggtgaaac ctgcacaggc
    60841 tgctgcaacg tcaaatgccg ggcaaccttt aatgtccaac atgctttgaa tctgacaagc
    60901 tgcgctcggg aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc caatctggtc
    60961 tttctcaatg cccgccatct caattgcgcg tgtcgccgct tcaaagccca tggttgaaac
    61021 ggtttcgttt ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga caatccactc
    61081 gtcagaggtg tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata
    61141 gctgccagta ccaataatct tcgtatacat gtacgctcag tcacttttcg gttatatacc
    61201 gtcacttgca aactgcgagt tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc
    61261 caccgtccag cagctcaaaa ccagctgggt atacagattc caggcgagcg gcaattcgct
    61321 gaggaacttg tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca aaagctcgct
    61381 gattggctgc accatgactt tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt
    61441 tatactggtc ggggttgagg tgactgaatc gcctcgtcag gctcttttgt agccaacgct
    61501 ttaataacag tagccaccac gaccgttttt tcccttcacc ctgagatttc agcagagaaa
    61561 ggaacatcct gacaacacct tccatcgtct ttaacgtgac atttcctgta aagccgtcac
    61621 aaaccagcac atctgtcttg ccagttaaca actcattggc ttcaagatag ccgatataat
    61681 tgatagaagg gattgtttta agcaccgctg aggcatcccg aatactgtca agacccttta
    61741 cttcttcttc accaatattg agcaacgcca cgcgaggatt gggaatttcc accacctctt
    61801 cagccagaac tgagcccata atggcaaatt gcaccaacat tgtgctgtca caatcgacgt
    61861 tggcccctaa atcgaggacc accgttttgc ccttttgctg atgtggtaat accgtcacca
    61921 gcgccggacg ctcaatcccc tccaggggct tgagtaataa ttttgccagc cccatcagtg
    61981 ccccggtatt accggcactg acacaggctt gcgctcgacc ttctttcacc agctccaggg
    62041 ccatacgcat tgaactccca cggctggcgc ggatagcttg cgaaggccgg gcatcactgg
    62101 cgataactga ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag tcagctttag
    62161 caagtaatgg cgtgatggcg tcgggattgc cgactaaaag aagagtgagt tgcgaattag
    62221 aattcagtgc ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc cctcccatga
    62281 catctaacgc cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc
    62341 agttgcctgg ggaaaatcct cactaagctt catcacgcag acgcgtgatt acttagcgat
    62401 gaccttgcgg ccgcggtagt aaccgtcggc agtgatgtgg tgacgcaggt gtttttcacc
    62461 agaagttttg tctacagaca ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat
    62521 gccacgtttg gaacgggttg gtttattctg ttgtacggcc atggacctta ctcctcaatt
    62581 acggtttatc cccgatccaa cggggagcac caattactta cgctttaagc tggctaatac
    62641 ggcaaatggg tttggctttt gcacttcttc aggcagttca ccaaagacca tgtccgcttc
    62701 ggacacttca cagtgttcag aatcatgcac cggaactacc ggcaaggcga ggatgatttc
    62761 atcttcaacc attgcaagca gatcgatttc accgaattcg ttaacctcaa tcggttcata
    62821 cgcttccggc agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt
    62881 gtagacctga tgagtaaacg gcttcccgca acgctgacac tcgagcgtta ccgtcacctt
    62941 cgcatcgccg tttaacactg cgagacgttg gttatcgata gcgaacgaca tggagcattc
    63001 cacatcactg tccacactga ctacggattc ggcgacgcgc tcaacctgat caggggtata
    63061 gataccctgg taatcaaggc gtttttgagc cgtacgaacc ggatcgagag tcaggggtaa
    63121 ttttaccttt tgcatagggc gcgcatatta actttgtaac gtcatagagt caaagaaaaa
    63181 ggcagcctgt ggttgccttt tgccaataat tcgcacacat tgcgggttac tgctttattt
    63241 tcgttcagtg ctgataacga acatcaacct gcaaaacgaa gcgcatagtt taaaatggct
    63301 atcatcaata cgctatatct gatggaaaaa aatatgccta aacttatttt agcctccacc
    63361 tcgccatggc gtcgcgctct tctggaaaaa ctgcaaatct cttttgaatg tgcagcacct
    63421 gaggtcgacg aaaccccacg cagcgacgaa tcaccgcgac agttggtgct tcgactggca
    63481 caagaaaaat cgcaatctct ggcgtcacgt tatccggatc atttaattat tggttcagac
    63541 caggtatgtg ttcttgatgg cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt
    63601 ctgcaattac gcaaagccag cggcaatatc gtcaccttct atactggact ggcgttgttt
    63661 aattcggcga atgggcatct acaaacagaa gtggagcctt ttgacgtcca tttccgtcac
    63721 ctaagcgagg cggagattga taattacgtg cgtaaggagc atcccctgca ctgcgcgggt
    63781 agctttaaga gtgaaggatt tggcattacg ctgtttgagc gtttagaggg acgtgaccct
    63841 aacacgctgg ttggtttacc gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa
    63901 aacccgctga tgggatagtt gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt
    63961 gcaggcggcc tgatgcggcg taaacgcctt atcaggccaa ccgcccatta tcaggcttat
    64021 atgcttgtat tagcgcgcgt tacgcagctt ttgcaaacaa cgcttcaaac cttcatccat
    64081 cggcgcttcg atacgcatca cctcaccggt ccccggatgg gtaaacttca acgctgcggc
    64141 gtgcaggaac agacgattta atcccgtgcc tgcttcagtg agctgtctgt caaattcacg
    64201 gtcaccgtag cgatcgtcaa aggcaatcgg atgacccgca tactgtgtat gcacacggat
    64261 ctgatgagta cgtccggtta ccgggctaca acgcaccagg gtggcaaatg catagcgttc
    64321 ttccacttta aagcgtgttt ccgacggttt gccttcctga ctgacacgca cgatacgttc
    64381 gccgctttgc agaatatttt tcaataacgg cgcttgaacg ctcttcacat gcgactgcca
    64441 ctgaccgcgc accagcgcca ggtaatcttt ttgcattcct ttttcacgta attgctcatg
    64501 cagagaacgc aacgccgagc gttttttcgc taccagcaac acacctgaag tgtcccggtc
    64561 gagacgatga accagttcaa ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat
    64621 aacgccgaag cttaaaccac tgccgccatg taccgccgta ccggaaggtt tattcagcac
    64681 caggatatga tcatcttcat ataagatgac gtccgccagc gccgccacct tttgcagatg
    64741 tggcgaaacc gcctcttctt cccgctcagc aacgcgaacc ggtgggatac gcacctcatc
    64801 acccgcttcg agtttatatt caggcttaat acgttttttg ttcacccgca cttcaccttt
    64861 acgcaaaata cggtaaatca tactttttgg tacgcctttc aattgggtac gcaaaaagtt
    64921 atcgatacgt tgccccgctt cgtcagcggt gatagcaaca atttttacgg atggagtctc
    64981 tgttttcatg gtgggcgatt ctaattagcc aacaggattc gcgccactca tttttctatg
    65041 cttatattta gttttgcacc ttattacttc actacgtgat cactttattg atggttatta
    65101 aaccaatcac cagcaagaag tgaaaaaact gtgagtaagc gggtgataaa tggtaaaagt
    65161 catcttgcta taacaaggct tgcagtggaa taatgaggcc gtttccgtgt ccatccttgt
    65221 taaaacaaga aattttacgg aataacccat tttgcccgac cgatcatcca cgcagcaatg
    65281 gcgtaagacg tattgatctt tcaggcagtt agcgggctgc gggttgcagt ccttaccggt
    65341 agatggaaat atttctggag agtaataccc agtctgtttc tttgataatt gcgctgtttt
    65401 tccgcatgaa aaacgggcaa ccgacactct gcgcctcttt gagctgacga taaccgtgag
    65461 gttggcgacg cgactagaca cgaggccatc ggttcacacc cggaaaggcg ttactttgcc
    65521 cgcagcttag tcgtcaatgt aagaataatg agtaagttac gatgaaaaga atgttaatca
    65581 acgcaactca gcaggaagag ttgcgcgttg cccttgtaga tgggcagcgt ctgtatgacc
    65641 tggatatcga aagtccaggg cacgagcaga aaaaggcaaa catctacaaa ggtaaaatca
    65701 cccgcattga accgagtctg gaagctgctt ttgttgatta cggcgctgaa cgtcacggtt
    65761 tcctcccact aaaagaaatt gcccgcgaat atttccctgc taactacagt gctcatggtc
    65821 gtcccaacat taaagatgtg ttgcgtgaag gtcaggaagt cattgttcag atcgataaag
    65881 aagagcgcgg caacaaaggc gcggcattaa ccacctttat cagtctggcg ggtagctatc
    65941 tggttctgat gccaaataac ccgcgcgcag gtggcatttc tcgccgtatc gaaggcgatg
    66001 accgtaccga attaaaagaa gcactggcaa gccttgaact gccggaaggc atggggctta
    66061 tcgtgcgcac cgctggcgta ggcaaatctg ctgaggcgct gcaatgggat ttaagcttcc
    66121 gcctgaaaca ctgggaagcc atcaaaaaag ccgctgaaag ccgcccggct ccgttcctga
    66181 ttcatcagga gagcaacgta atcgttcgcg ccttccgcga ttatttacgt caggatatcg
    66241 gcgaaatcct tatcgataac ccgaaagtcc tcgaactggc acgtcagcat atcgctgcat
    66301 taggtcgccc ggatttcagc agcaaaatca aactgtacac cggcgagatc ccgctgttca
    66361 gccactacca gatcgagtcg cagatcgagt ccgccttcca gcgtgaagtc cgtctgccat
    66421 ccggtggttc cattgttatc gacagcaccg aagcgttaac agccatcgac atcaactccg
    66481 cacgcgcgac ccgcggcggc gatatcgaag aaactgcgtt taacaccaac ctcgaagccg
    66541 ccgatgagat tgctcgtcag ctgcgcctgc gtgacctcgg cggcctgatt gttatcgact
    66601 tcatcgacat gacgccagta cgccaccagc gtgcggtaga aaaccgtctg cgtgaagcgg
    66661 tgcgtcagga ccgtgcgcgt attcaaatca gccatatttc tcgctttggc ctgctggaaa
    66721 tgtcccgcca gcgcctgagc ccatcactgg gtgaatccag ccatcacgtc tgcccgcgct
    66781 gctccggtac cggtaccgtg cgtgacaacg aatcgctgtc gctctctatt ctgcgtttga
    66841 tcgaagaaga agcgctgaaa gagaacaccc aggaagttca cgccattgtt cctgtgccaa
    66901 tcgcttctta tctgctgaat gaaaaacgtt ctgcggtgaa tgcgattgaa acgcgtcagg
    66961 acggtgttcg ctgcgtgatt gtgccaaacg atcagatgga aaccccgcac taccacgtgt
    67021 tgcgcgtgcg caaaggggaa gaaacgccga ccttaagcta catgctgccg aagctgcatg
    67081 aagaagcgat ggcgctgccg tctgaagaag agttcgctga acgtaagcgt cctgaacaac
    67141 ctgcactggc aacctttgcc atgccggatg taccgccagc gccaacccca gctgaacctg
    67201 ccgcgactgt cgtagcccca gcaccgaaag ctgcaacggc aacaccagca gctcctgcac
    67261 aacctgggct gttaagccgc ttcttcggcg cactgaaagc gctgttcagc ggtggtgaag
    67321 aagccaaacc gaccgagcaa ccaacaccga aagcagaagc gaaaccggaa cgtcaacagg
    67381 atcgtcgcaa acctcgtcag agcaaccgcc gtgaccgtaa tgagcgccgc gacacccgta
    67441 gtgaacgtac tgaaggcagc gataatcgcg aagaaaaccg tcgtaatcgt cgccaggcac
    67501 agcagcaaac tgccgagacg cgtgagagcc gtcagcaggc tgaggtaacg gaaaaagcac
    67561 gtaccaccga cgagcagcaa gcgccgcgtc gtgaacgtag ccgccgccgt aacgatgata
    67621 aacgtcaggc gcaacaagaa gcgaaggcgc tgaatgttga agagcaatct gttcaggaaa
    67681 ccgaacagga agaacgtgta cgtccggttc agccgcgtcg taaacagcgt cagctcaatc
    67741 agaaagtgcg ttacgagcaa agcgtagccg aagaagcggt agtcgcaccg gtggttgaag
    67801 aaactgccgc tgccgaacca attgttcagg aagctccagc tccacgcaca gaactggtga
    67861 aagtcccgct gccagtcgta gcgcaaactg caccagaaca gcaagaagag aacaatgctg
    67921 ataaccgtga caacggtggc atgccgcgtc gttctcgccg ctcgcctcgt cacctgcgcg
    67981 tgagtggtca gcgtcgtcgt cgctatcgtg acgagcgtta tccaacccag tcgccaatgc
    68041 cgttgaccgt agcgtgcgcg tctccggaac tggcctctgg caaagtctgg atccgctatc
    68101 caattgtacg tccgcaagat gtacaagttg aagagcagcg cgaacaggaa gaagtacaag
    68161 tgcagccgat ggtgactgag gtccctgtcg ccgccgctgt cgaacctgtt gttagcgcgc
    68221 cagttgttga agaaatggct gaagtcgtag aagcccccgt accggttgcc gaaccgcaac
    68281 cggaagtggt tgaaacgacg catcctgaag tgattgccgc cgcggtaact gaacagccgc
    68341 aggtgattac cgagtctgat gttgccgtag cccaggaagt cgcagaacac gcagaaccga
    68401 tggttgaacc gcaggaagag acggcagatg cagatattga agaagttgcc gaaactgctg
    68461 aggttgtggt tgctgaacct gaagttgttg ctcaacctgc cgcgccagtc gtcgctgaag
    68521 tcgcagcaga agttgaaacg gtggccgcgg ttgaaccaga gatcaccgtt gagcataacc
    68581 acgctaccgc gccaatgacg cgcgctccgg caccggaata tgttccggag gcaccgcgtc
    68641 acagtgactg gcagcgccct acttttgcct tcgaaggtaa aggtgccgca ggtggtcata
    68701 cggcaacgca tcatgcctct gccgctcctg cgcgtccgca acctgttgag taataattag
    68761 ctcaaagtaa tcaagccctg gtaactgcca gggctttttt atttcatgtt tgaatcaaca
    68821 tattaaaatt catcaaatgg catccttgct aaccaacaat gcaaaatagg caaaaaaaat
    68881 gcccgccccg gcgatcccag agcgggcaga caaaacatac ccagtttcat gatatgtttt
    68941 aaagcgagct tatttgttga gctggaagag cgacaatccc tgcatatcgg taaatgcttt
    69001 atacgatgcc tgcaatgccg tttgctgcat gatgtaagat gaaatagttg cattccagtc
    69061 cacatcaacc agatcgctca tctgctgcgt ttgccctaaa gcgcgatcgc tacctaagga
    69121 atccagcgac tccagtttgt tcagctgcgt gcctaattcc gcgcgaacgg taaggacatt
    69181 attcagcgag tttttcagtc cacggttggt tttatccaat gccgcagcgg cgatttcttt
    69241 atccgcttcg ctatccgcga ccggcgtttt cagggctgcg atggcgctat ccagcatggc
    69301 aaaaagattg gtttcagaag cgctaccgtc tggttccgct accgcgttac tggtaatact
    69361 gtcgaaaatt ttgtcgcccg tatgtcctat caccatcgaa cgcgaagcat cgacctgttg
    69421 cttaatactt tctgttccgc caacataatc accgttagcc tcactaaacg gcgcagtctc
    69481 tgttttatat gtagcgccca ttaccgtcag tggtgttcgc cagattcagc aactggtcac
    69541 gaagcccctg aatatccgta gccagcgagg cccggtcatc gtcactcaag gtgccattgc
    69601 tggcgtagac aattttttcc tgagcattct ggatagcagt ggtgacctgg ctaagtacac
    69661 tctcttccag tgacactttt tgagtggcga aagtacgtgc cagcgtgtac tggctgtttt
    69721 gcgcctgtgc ctgggagaga acaacagctt gtgatgcagc aatgggatcg tctgaagggt
    69781 taacgactcg cttacccgtc gacatctgct cgccgtactt catccattct gcctgagaat
    69841 tggtgatgcc gcgcatgttt tgctggtaca tcatctgggt actgaaacgc attttatctc
    69901 cccttagcga atgttaatca gcgcatcaaa aatcgcgttt gccgtctgca gaacctgcgc
    69961 attcgccagg taatactgct gaaaacgttg cagatttccg tactcctcat cgagattgac
    70021 accggaaatc gactgctgct gattggaaag ctgcgtcacc acattacctt gcgtggtgct
    70081 actggttttc aacgtcgcgg ttttattacc gatatcactc actaacgagg cataagcgtc
    70141 gttaaaggat ttcgcaccgc ccaccgtttt actgttgctt tgcagatcca gcagggcctg
    70201 accgttgcgg ttgtcgctat cacccgcatc ttcttcgctc gccatcgcga ttttcgcttc
    70261 gtcggtgatt aatacatcca tgttgacgat ggcgtcactt actggtttca gcgtgaagct
    70321 gtcgttaacg gcaggcgttc ctgtaaacgt caactccaga ccatcaaatg ccactttacc
    70381 gttggcatct ggtgtcgccg taaaagtggt attgctggca aggcgggtga cctgccactg
    70441 attattatcg aacgagattt tgtaatctgt cgccagtacc acggaggcat cagttaccgt
    70501 ggcaccgatc gcaacgtcac ctttgttttt cgtgttttgc agaaccgcgg gcttaccgat
    70561 agtaaagaaa tcttcaccag catcgccgtt agcatcaaat ccggctttgt gttggctgtt
    70621 gaaagcctcg gcaaatgcca gcgccagttg tccaagcgta ttacgcgtct ggtccagatc
    70681 ctgagaacgg aatgtcagaa tgccgcccag cgacccggta ttcagtaatt tctccgggat
    70741 ctcaatattg cctgccgtcc catcaacgta agcgacagtc gtacgagaag ggtcggcgct
    70801 ggaaggaact gccgccagtt gccgcgccgt acttccctga accagtgagt aaccattggc
    70861 atcgtgatgt tataagtacc gccatcctga acgctgactt ctacgccaac aatctggttt
    70921 aattcgctca ccagttgatc gcgttgatcc agcagattgt taggtgacgc ccctgccccc
    70981 acgcctgtca ggcgcgagat ttgatcgttc aggctggcaa tttgtttagc gtaattgttg
    71041 atctgatcaa cgctggcacc tatcgcgata ttgacctgtt tgtcctggtc gcgcagatat
    71101 tgatcggtgg ttttaaactg attcaccaac ccttctgatt tcccaatcag cgcctggcgc
    71161 gccgccgggt cttccgcgtt actcaccagc gtttgcaggc tggtgaagaa atcctgcatc
    71221 tgtgttgcca gcgaagaggt actggtggag agcatattgt cgattttcga catctgctca
    71281 tagcgggcgg tcaggccgct actttgcgtc tgcgcagcac gtaactggtt ggtaataaac
    71341 gtatcatact cacgctgcac accagaaacg tagacgccat tgccaaccca gccgccagcg
    71401 cccaacgtgc tattggcctg cgccataata gtggtttggc gggtatatcc ggcaacgtta
    71461 tagctggaga tattattact tgccgtattt aacgccgcct gggccgcgtt cagtccgctc
    71521 atggcgttat tgatcaagct ggacatggag gttccttata agccttcaat tacagagtat
    71581 tatcggcagc gacccgccgg acttgagtta ttcagaacag attatcaatg ttcatgctgt
    71641 aggttttgct caccttgtcg cttatcgatt tcatctgctg gatcatgttg gtgagtttac
    71701 gggcatagtg aggatcggta gcatagcccg cgtcctgtag gacctgcgcc ccctgttccg
    71761 cactcgcggc ggtcgtcacg gcggcgtagc gcgggttacg tgttaacagc ccaacgtaat
    71821 ccgacaatgc ttccagatac gagctgtaga cccgaaactt cgcttttact ttcttcgctt
    71881 cgccgttttc atattcagtc gtggtgattt cagtgacttg ccctttccag ttgccagagg
    71941 ctttgacacc aaacaggtta tagcttggct cgccgttttc gcggcggatt tgccgttgcc
    72001 cccaaccaga ttcaagcgca gcctgagcga ggatcaaatg atgtggcaca ccgctttgct
    72061 ggcttgccag ttgggcgggc agcgagagtt gcgcgaggaa tgctctactg tcacccggca
    72121 gcgaatcatc gtagttacgt ggtacggcct tttgcaccag ctgcgaaagc gtctggtttt
    72181 gataacgcac cacggtttcg agcgggaatt tcatcggtgc tgctggcgtg gactcctctg
    72241 gcaatggttg ttctggcgtc atctgtttaa ccatcatctc tgcaagcccc agacctttgc
    72301 ccgccgtcat ctgttgggca atttgctggt catacatact ggtatacaga cgagtgtgct
    72361 cgctgctgaa caggccatct tttggtaaag cgtcgcgcat gcttttcaac atcatctgca
    72421 cgaacatccc ttccacctga cgggccaccg gacggatatt tgccgccgga tcttcgctcg
    72481 ctttcgcctt tagttcgttg agtgattgcg catcccaggc cgcacttgcc agtagtttgc
    72541 tgtcgctgat catcagatga tttccagttt tgcccgcaga catcccgcac tttgcattga
    72601 ttgcaaaata gacatcagat ccatcggcgt agcgcccagc gcattgagcg cacgcaccac
    72661 gttattgagg ctggcgctgg aacgtacgct ttgcagcgaa ccgccgctct ggcgtaaatc
    72721 gatctgcgtt tgtggagtaa caacagtctg tccaccacca aacggtgtat ctggctggct
    72781 gacattggcc tgacgattaa ctgttactga gagatttccc tgcgctaccg cgcagctgtc
    72841 gagggtcact tcgcgattca tcaccaccga accggtgcgc gagttaatca ctactttagc
    72901 gtcctgcggg gtgacattaa cctgcatatt ctggatatcg gcaaggaaac ggacctggga
    72961 actgttgcca cttggtacgc gcacctgaat agtccgcgca tctaacgcgg tggcgctgcc
    73021 atatccacgc acgcggttga tggtgtcagc gatttgctgc gccatgctga aatcttcgtc
    73081 gttaagttgc aaattaaggg tattcccgac gccaaactgg ctgggcaatt cacgttcaat
    73141 aaccgcacca ttggtgatcc gtccaccgtt cagttggtta acctgaacac tgctaccgcc
    73201 tgcggaggct cctgcgccgc caaccagaat attgccctgc gccagcgcat acacctgact
    73261 gtcaacgccc ttaagcggag tcatcaacaa cgtacctcca cgcaagcttt tggcattacc
    73321 catggaagaa accaccacat cgatggtttg cccctgacgt ccaaacggag gaagtgacac
    73381 tgtcaccatt accgcagcga cgttttttag ctgcatattg gtgcccgtcg gaacggtaat
    73441 tcccagctgt gagagcatgt tattaagcgt ttgtgtggta aacggcgtct gggttgtctg
    73501 gtcaccagtg ccatccagcc ccactaccaa gccatagcca atcagtgagt tttgccttac
    73561 cccctgaaca ctggtgagat cgcgaatacg ctcagcctga gccgccgtcg taaccagtag
    73621 aagaattaat gcagagagaa atttaatcac cacagcctca cttacattgg cgacaggtta
    73681 aggaagaaac gctgcaacca gcccatattt tgcgcttcgt taatgtagcc attgcctacg
    73741 tattcaatac gcgcatccgc cacctgagta gacggtacgg tattgctgcc gctgatagtg
    73801 cgtggattaa ccacgcccga gaagcgaata aattcggtac cctgattaat ggcaatctgt
    73861 ttttcaccca ccacatgcag gttgccgttg accagtacct ggtcaaccgt caccgtcaac
    73921 gtgccgctaa aggtattgct ggcattggcc ccgccctttc cgttgaacgt gttaccaccg
    73981 gaggcttcga catcggcacg agcgttacca aacagcccct gcaaatagcg cggcacagta
    74041 tcaaagccaa aattagtttt accgtcacgg ctggcattcg cagaggagct tttgctggcg
    74101 ctgacgttct cctgcaacac gatggtcagc gtatcgccaa tattgcgtgg tcgacgatct
    74161 tcaaacagcg gttgatagcc atagttaatc ggctgagcag actggaaaat agaaccgttg
    74221 gcgacgggcg tcggaccggg aaccggttgt gcactggtcg ccccctgcac cagcggcgtg
    74281 gagggtatcc aggcgcagcc ggttagtgaa agcaccaaca agctggaaat ggcataagta
    74341 tgcgcagcgt ttttttgcat ggctatcatc ttcaaaaatc agtaaaccgg tgaacctgcc
    74401 accggttatg ccttagagtt gcgtcagttt ttgcagcatc tgatcggtgg tggacaccgc
    74461 tttactgttg atttcgtaag cgcgttgcac ctgaatcata ttgaccagtt cttccgccac
    74521 gttgacgtta gacgtttcaa catacccttg atacagcagt cccgcgccgt tcaggcccgg
    74581 cgtgctttcg ttcggtgcac cggaggattg cgtttcggtg tagaggtttt cgccaatgct
    74641 ctccagtccg gtatcattca taaaggtggt gagattgagc tgcccaacct gaaccggagc
    74701 tgcctggcct tgttgggtta cgctgaccac gccatcacga ccgatggtga tacttaacgc
    74761 attcgccgga atggtgatcg ctggctgcac ctgaaaacca ccagccgtca ccagctgccc
    74821 gttctgatcc acctggaaag agccgtcacg gatataggct gatgagccat ctggcaacat
    74881 tacctggaaa aagccctgcc ctttaatcgc gacatctttg ctgttattgg tctgcgacag
    74941 attgccctgg ctgtgtaagc gttcagtggc gaccgggcgt acccccgtgc cgatttgtaa
    75001 tccggagggt aaggtggttt gttcggaaga ctgtgccccc ggctggcgaa tggtttgata
    75061 aagcagatct tcaaacaccg cgcgctgacg cttaaaaccg ttagtactga cgtttgccag
    75121 gttgttggca atgacgtcca tattggtttg ctgggcgtca aggcccgttt tggcgatcca
    75181 taatgaactg atcatataat gtcctgtagt ggattgctga caagcagagc agaagcacgc
    75241 gacagtgtga atttcccctc accctaaccc tctccccaaa ggggcgaggg aatagtccgc
    75301 gcgcttttat tgcaatgtca tcaatcgttc aattaactca tcgacagcag ttggttggca
    75361 cggcctgcgt tatcatcgac gctgctgatc accttcatct gcatttcaaa acgccgcgcg
    75421 ctggcaatca tgtcgctcat tgccgcaacg gcattgacgt tactgccttc cagaaccccc
    75481 gacatcacac gcaaggttgg atctgcctgc agtaccggcc ctcgcgtggc ctgggtttct
    75541 gcgcttaaac gaaaaatgcc gtcgtcaccg cgctgcactt cgctgcccgt ggctttcacc
    75601 agtttcagac gccctactgg cgcaactgta tttgccggat cgcccggatt gagcgccgag
    75661 attgtgccat cggcagcaat cgtgatttcc gccccttccg gcacagcaat tggccccgcc
    75721 tcgcctatca ccggatgccc ctgaattgtc agttgcccgg tgggatcaac ctgaatgctg
    75781 ccattacgcg tatacccttc gctgccgtca gcggtctgca cggccagcca tcctgctgca
    75841 acgcaacgtc cagcgggcga gaggtgtaat ccatcttgcc gggcgtcatg tctgcacccg
    75901 gtgttgacgc cgttaccagc gtgcgcgtgg gcagtgaaag cccttccacc ggcactgcgc
    75961 gcaaggcgtt taactgcgcg cgaaaaccgg gcgtagaggc attggccaga ttactggcgg
    76021 ttaccgcctg ttgattcagc gtctggctgg ctgctcccat cgcagtataa attgcgtgat
    76081 ccattgagct atcccgtcag cgattagcgt aagttaaccc gcgtgttgag gatctggtcc
    76141 tgggttttga tggtctgggc gttagactta tagttacgct gggcaacgat catattgacc
    76201 agttctttac tgagatcgac gttggacgct tccagcgcac cgttggtcag agtgccaaag
    76261 tttcccgtcc cggctgtccc caacagcgcc acgccagaag attgcgtcgc agaccagacg
    76321 ttgtcgcctt cggatgccag accttcgttg ttggcaaagt tcgccagtac aatctgcccc
    76381 agcagttggg tttgttcgtt ggaattgttg ccgacaaccg taccgtcatc attgatttga
    76441 taactcacca gatcgcccgg tttgtagcca ttctgggtgg ttgccacaat gttgttagcg
    76501 ccggtatttt gctgcatgga gttgaggaag ctcagactaa acgtggcggg ttctgcaccg
    76561 ttaattgcgc cggttgcgat attattcgcc atcgcaccat ccactaatgt gccattagca
    76621 ttaaattcca gtgttgtcgc tgtcttcgca atgctgtttg gatcactgct atcctgggtg
    76681 tagacctgcc agttattatc cccggtcttc acaaaataga cgctcatgtc atgagcatta
    76741 ccctgactgt cgaaaacagt caccgaacct tttttgttat agctatccgc attgctggcg
    76801 ctgaatggcg taacagtagg aagcggatca ctggaattca ggttgatctg catcgacgcc
    76861 gtggtggtag ttttcgctgc catcagggta ttcgggatcg aaatattggt cggattcgcc
    76921 ccttgctgaa tagtcggcgg cgtaccggtt accgggtaac ccgtcagctg taaaccttgc
    76981 gtattcaaca ggttacggtt ttcatccagc ttaaattgtc cgttacggct gtagaacacc
    77041 gaaccgttgc tgtctaccag acggaaaaaa ccgttctggc tgatagcaac gtccagacct
    77101 cgcccggtgt tggtggtcgt gccatcggta aagtcctgag tgataccggc aacttttacc
    77161 cccagtccca ctttcgaacc ggcaaacata tcggcaaaag aggccgtgcc tgatttaaag
    77221 ccgtaggtgg cggagttggc gatattgttg ccaataacat cgaggttggt ggcggcagcg
    77281 tttaatccgc taaccgcttg agaaaaggcc atgactgact cctgaaatgt gaaggcttaa
    77341 attatctgcc gtacttcgtc gagggtggtg gtgccgtaag tgccgagatc cagcgtatta
    77401 ccgctgttgc cgcggatcac accctgcacc agagcaaact gcagcggctg ggcaaccagt
    77461 tgtgtaccac cgttactggc gctaatcgct acattgtaag aaccgttcgg cgcagtgctg
    77521 ccatcagtca acgaaccgtc ccaggtgaaa ctgtgaactc cggcggtcag ttcaccaata
    77581 tcaatggtgc gcacaaccgc gccattttta tcggtgatgg tggccgtaac tttgtctgcc
    77641 gcctgttgca gctcaacacc aaacggcgtg gtcgtggtca cagccccttc ttcactgccg
    77701 gttcccgcaa gaacagttgt gccggggatc atcacgccgt gaccgatcag gttactggcc
    77761 tgtaacgact ggctgttatc aatctgtccg gaaatagatc cgagcgtggt attgagtttt
    77821 tcaatcccgc tgaccgtgct gatttgtgcc aattgcgacg tcagctcgtt gttttccatt
    77881 ggattggtcg ggtcctggtt tttcagctgc gccaccagca aagtcagaaa actgctttgt
    77941 aaatctgcgg cgttgctgcc cgtgagcgaa ctactgctgg tggtactgac gccggtattt
    78001 gtcggatcgg tggtggttac cgcaatggac atagctttct cctttattga ccgagcgtaa
    78061 gggttttcag catcatgctt ttcaccgtgt tgagcacttc aacattggcc tgatagctgc
    78121 gtgacgccga catggtgtta accatctccc cgacgacatc aacgttcggc atttttatgt
    78181 agccctttgc atctgccagc ggattacctg gttcataaac cagtttgtcc ggggcctgac
    78241 tttcttaaca tcggcaacct ttacgccgcc tgtcgcagca cctggtgcag cgttaacctg
    78301 gaataccacc tgttttgccc gatatggctg tccatcagga ccggtcacgc tatcagcatt
    78361 tgccagatta ctggccgcca cgttcaggcg ctgggactgg gcagttaacg ccgacccggc
    78421 gatatcaaaa atattcagca gtgccatccg ttaatttccg ctctgtaaaa cgttcatcat
    78481 gcctttgatt tgcccgctca acgcgctaag gctcatctgg tattgcaggc tgttatcggc
    78541 aaactgggtg cgttcgcgat ccatatcgac ggtattaccg tcaagcgaag gctggtccgg
    78601 aatacggtat tgcagttctg cggtaggagg cgtcagcgcc tgcgccggga tgtgctgcgt
    78661 tgaggtcatc gtcagtgcaa ccacactggt tgcatcccgt ccacgttgca tgactttttt
    78721 aagttcactg gcaaaatcga tatcgcgcgc ctgataacca ggggtatcgg cattggcgat
    78781 gtttgctgcc agcacttcct gacgctgggc gcgcagattg agcgcctctt gttgaaaacg
    78841 taaggcggcg tcgagcttat cgagcatatc tcctccgcag gtatcaaaat tctgccatca
    78901 gcttaaatgc ctttacgcgc gcgttatcgg cggaataaac gcaaaatggg tcgctattta
    78961 tgccgttgat ggtcattgcg gacaggtaca attcacgttg tagaaatggc tgggggcgaa
    79021 aatgctgaca ataaaacgta gcgtggcgat catcgcgata ctgttcagtc cgttaagtac
    79081 ggcgagcaat ctcacatcgc aattgcacaa cttttttagc gcccaactcg cgggggtaag
    79141 tgatgaggtt cgtgtttcta ttcgtacagc gcccaatcta ctaccgccat gcgagcagcc
    79201 attgctttcg atgagcaata attcccgcct gtggggcaat gtgaatgtgt tggcacgctg
    79261 cggtaacgac aaacgatatt tacaggttaa tgtgcaggcc acaggaaatt atgtggttgc
    79321 cgcgatgcct attgtgcggg gaggaaagct ggaagctggc aatgtcaaac tgaaacgtgg
    79381 acgcctggat accctgccac cgcgtacggt gctggatatc aatcaacttg ttgatgccgt
    79441 tagcctgcgc gatctatcac ccgatcaacc tatccagtta acccagtttc gccaggcatg
    79501 gcgggtaaaa gcgggacaac gcgtcaatgt gatcgccagc ggtgatgggt ttagcgccaa
    79561 cgcagaaggt caggcgctga acaatgcagc cgtcgcacag aatgcgcggg tgcgcatggt
    79621 atcggaacag gtagtcagcg gcgttgttga tgcagatggg aatattctta taaacctgta
    79681 agctgttaaa gattacccgt cccttgccga taaataagca acacatgata aaagcgccct
    79741 caatgaggaa taaaccatga gtattgatcg cacttcgcct ctgaagcctg taagcaccgt
    79801 tcaaccgcgc gaaaccaccg acgcgccggt aacgaacagc cgggcggcaa aaacaaccgc
    79861 ctccaccagc accagtgtga cgttaagcga cgcgcaagca aaactgatgc aacccggcag
    79921 cagtgatatc aatcttgaac gtgtcgaagc gttaaaactg gcgattcgta acggtgaact
    79981 aaaaatggac accggcaaaa ttgccgatgc gctgatcaac gaagcgcagc aagacttgca
    80041 gagtaactga tcgtatgaca cgtcttgcag agatcctcga ccagatgtcc gctgtgctta
    80101 acgatctcaa aacggtaatg gatcaagagc agcaacatct ctctatgggg cagatcaacg
    80161 gcagccagtt gcaatggatt acagaacaaa aaagctcact gctggcgacg ctggattacc
    80221 tcgaacagtt acgcaagaaa gaacccaata cagcaaatag cgttgatatt agtcaacgct
    80281 ggcaggaaat tactgtgaaa acgcagcaac tacgccaaat gaatcaacat aacggctggt
    80341 tactggaagg acagattgag cgcaatcaac aggcgctgga aatgttgaaa ccgcatcagg
    80401 aaccgacgct atatggggcg aacggtcaga cctcaacacc ccatcgcggc agtaaaaaga
    80461 tttcgatctg aatattatct ccggcctgca ctgcaggccg gaatgcattg ttacaccgtc
    80521 cggcgggcaa attctttaac tttgaagccc agtaccgcca gtgcagcgaa gtacgcggca
    80581 atccccgcca gcacgaccgc cattaaacgc agtaagcgcc agggcatggt acccaatgac
    80641 cactccggca tgatatgtaa catacctaaa agcacgccag acatcaccag taccgccacc
    80701 accagacgca acagaaacgc catccagccg ggttgcggag taaagatttt ctgcttacgc
    80761 aactgccagt aaagcagcga agcattcaga cacgccgcca gaccaataga aagtgacagc
    80821 ccggcatgtt tcaacggacc aataaacgcc aggttcatca attgcgttaa aatcagcgta
    80881 acgatggcaa ttttcactgg cgttttaatg tcctggcggg aataaaagcc aggagccaac
    80941 actttcacta caatcaggcc aatcaaaccc accgagtagg caattaacgc ccgctgggtc
    81001 atcagcgcat caaacgcggt aaatttaccg tactggaaca gcgaaacggt taacggacct
    81061 gaaagaatgc ccaacgcaac cgcactcggc agtgccaaca ggaaacaaag acgcaacccc
    81121 cagtccatca aacggttgta ttcatcatga ttgccactgg caaaactttt cgacagcgac
    81181 ggcagcaaaa tggtgccaag cgccacgccc agcacaccgg agggaaactc cattaagcgg
    81241 tcggcgtaat acatccaaga caccgaaccg gaagcaagaa acgaggcaaa aatggtgttg
    81301 atgattaagg agatctggct cacagagacg ccaaggatcg ccggtcccat ctgtttcacc
    81361 acgcgcattg ctccagcatc gtggaagtta atgcgcggca ggaccagcat gccgatcttc
    81421 ttcaggtgcg gtagctgata caccagctgc agaatgccgc cgaccgtaac agcccacgcc
    81481 agcgccagca ccggtgggtt aaagtacggt gcggcaaaca gcgcgaaacc aatcatgctg
    81541 atgttaagca gtgttggcgc aaacgccgga atcgagaagc ggttccacgt attcagaatc
    81601 gctcccacca gcgacgccag ggagatcagc aagatatagg gaaaggtaat ctttagtagc
    81661 tggctggtca gggcaaattt gtcagctgtg tcagcgaagc ctggcgcggt caccatgatc
    81721 acccacggtg cggcgagcat gccagcgacc gtcacaaccg ccagcgcaag tgtcagcaag
    81781 ccagaaacat aagagacaaa gacccgcgtg gcgtcttcac cctgcttact tttatattcc
    81841 gccagaatcg gtacaaatgc ctgggaaaat gccccttcgg caaagatacg gcgtaacaag
    81901 ttaggaagtt taaaagcgac gaaaaaggcg tcggttgcca tccctgcgcc aaagattctg
    81961 gcgacaattg cgtctcgtgc gaagccaagc acacgcgaaa acatggtcat cgagctgacg
    82021 gcggccagcg attttaataa attcatcggt gttctaatcc ggacccacgg gcaaaacgcc
    82081 tgcaaggcag gcgctgaaag aggcgaatag tctacctaga ttatggtgaa ttgctaccgc
    82141 cagatgttac agggtttatt cactcatcgc atcgcgccag atcttgtcaa cgatacgttg
    82201 cgccagcact gcctattcgc cggcggtttg cggaactgtc tgattttaca cgcattcaat
    82261 gaagtgccgc gcacagccga caaatccacg ttgctcaaga gtgctctgcc aaccaggaat
    82321 cggtttattc actacaccct gcccgcgctc ctcacgccat tcgcgcatat ccgtaatgtc
    82381 gatgagcgca ccgtcagtca cggcctgcac ggtttcacgc tgacttccgg cacggcgatg
    82441 catacaagtg gtgatctgca aaggaccggc cgaaaaatgg tgctcggcaa acagcatttc
    82501 gccagcgtcg ttagtcagta gcgtaccgcc atccagagag gctttgccgc ccgacaacca
    82561 cagcgcggta tccaccacat gcagataatc atccagcaac gtgaaataaa gatcgtgtgg
    82621 cccgacgcta ttgctacgat gtttatccat tcttagcgag gctgcggtgg cgagttgcgt
    82681 ttttaactca ccgtagagtg gtgcgaaacg acggttaaaa ccgaccatca acgtcagttt
    82741 tttccgcgcc gccagttcca ccagcctttc agcatcgcgc agattttctg ccagcggttt
    82801 atcgacacag acatgtaccc ccgcattgag taacgtactg accacgtcaa agtggctggc
    82861 ggtgctggag tgcacaaaaa ccgcatcaca actggcggca aggctggata acgaatcggc
    82921 ataaggaatg cgccagctgt cacaaattgg cagggctttg gcgcgcgtag gcgaccaggc
    82981 tccttgtaac gtccagtcag acgctgccgc cagcaccggt aaccacgctt tttgcacaat
    83041 gccgcctaat cccactacgc cgatacgtaa ttttttcact ttaatctccc agatgggcca
    83101 gcaacgaatc aagacgttgt ttcagttctg ccacttcgat ttccagggct tcgacgcggg
    83161 tctgtaaatc accgtcaacc atgttcgaca tagccgttac cgccggctga tcttcaacct
    83221 caccactgaa aagatgcatg tagcggcttt cgcgtttacc cggttcgcgg gccagacgca
    83281 ccacaaaagg accatcttcg cgattcgcca gttgttccag cgtcgactcc acttccgcca
    83341 tatcgctgaa ttcatacatt cgcgcggcgc ggctgcgcag ttcacccggc gtctgggcac
    83401 cacgcaataa caacgtggtg attaacgcca cttctgcggc gctcagtttc agatcgccaa
    83461 attctgaatt acaaaaacgt tgctcatatt tggtgacccg attaccaaaa ccgctcactg
    83521 tgcgtagata atgacgtttg accagattat ccagctgttc ctgcacttcg gattcactca
    83581 gattcatgac cggttcacgg ttcgttttct gattacaggc cgtgactaca ccattgactg
    83641 agagcgggta ttgctccggc gtggtcacct gcttttccag caagcaacca attacgcgag
    83701 cttccagggc agtaagttga tatttcatga gattctcctt agcggccggg cgtccagtct
    83761 ggggtagtta atgccgtcag tacgtgatcg cgccattgtc catcaatcaa cagatagtct
    83821 ttcgcatagc cttctttttc aaaacccagt cgcgccagta aatcaccgct gcgtttattg
    83881 tgcggcatat aattagccat aatgcgatga atatgttggg tgcgctgcat ataacgaatg
    83941 gctgcggtca gggcttcaaa catgagtcct ttgccctgcc atttttgccc aatcgaataa
    84001 ccgagatagc aggcatgaaa agagccgcga acaacattgg aaaaattggc aacgccaata
    84061 atctcttttt cgtccgggtc gaataagcca aagtagaaag ctgaaccttg tttatgaaat
    84121 tcgttaatca tccctagcct ggcctgccag cctgatggat aacagtggct ttcgtcgcgc
    84181 actggctccc agggcttgag gaaatggcga ttctctgcgt aataatccgc cagacgccag
    84241 gcatcacgat catgcaccag acgcacgacc agtcggtctg tggttaagcg cacttttggc
    84301 acgttactgc gatagccaaa cattctatac ctactccttc ccgtaacgtc tttaatacgc
    84361 tgtaagtatt actatacctg cgcaaccaga ccctgtgaaa agtccaaaat gccattttct
    84421 gcattattca catatttgat aaaaactctc tatcaaagcg acgttttgat aaaaaaatac
    84481 tgtccccgaa gtgatgggaa aagtcttagc ggcaatgcag aatagaaagt gctcatcttt
    84541 tatttcccgg gaggggaaat gtcccgcgta tcgcaggcga ggaacctggg taaatatttc
    84601 ctgctcatcg ataatatgct ggtcgtgctg gggttctttg ttgtcttccc gctgatctct
    84661 atccgcttcg ttgatcaaat gggctgggcc gccgtcatgg tcggtattgc tctcggtcta
    84721 cgccagttta ttcagcaagg tctgggtatt ttcggcggcg caattgccga ccgctttggt
    84781 gccaaaccga tgattgttac cggtatgctg atgcgcgccg ccggattcgc cacaatgggt
    84841 atcgcccacg aaccgtggct attgtggttt tcatgcctgc tctcgggact cggcggcacg
    84901 ttgtttgatc cgccgcgttc ggcgctggtg gtgaaactaa tccgtccaca acaacgtggt
    84961 cgttttttct cgctgttgat gatgcaggac agtgccagtg cggtcattgg cgcattgttg
    85021 gggagctggc tgttgcaata cgactttcgc ctggtctgcg ccacaggggc cgttttgttt
    85081 gtgctatgtg cggcgttcaa tgcgtggttg ttaccggcat ggaaactctc caccgtacgc
    85141 acgcccgttc gcgaaggcat gacccgcgtg atgcgtgaca agcgttttgt cacctatgtc
    85201 ctgacgctgg cgggttacta catgctggct gtacaagtga tgctgatgct gccaattatg
    85261 gtcaacgacg tggctggcgc gccctctgcc gttaaatgga tgtatgccat tgaagcgtgt
    85321 ctgtcgttaa cgttgctcta ccctatcgcc cgctggagtg aaaagcattt tcgtctggaa
    85381 caccggttga tggctgggct gttgataatg tcattaagca tgatgccggt gggcatggtc
    85441 agcggcctac aacaactttt caccctgatt tgtctgtttt atatcgggtc gatcattgcc
    85501 gagcctgcgc gtgaaacctt aagtgcttcg ctggcggacg caagagctcg cggcagctat
    85561 atggggttta gccgtctggg tctggcgatt ggcggcgcta ttggttatat cggtggtggc
    85621 tggctgtttg acctgggcaa atcggcgcac cagccagagc ttccgtggat gatgctggga
    85681 attattggca tcttcacttt ccttgcgctg ggttggcagt ttagtcagaa acgcgccgcg
    85741 cgtcgtttgc tggaacgcga cgcctgattt gatactcccc cctgactcct ccatactgaa
    85801 agtgaactga cagccaaatg gaggagtcac gtgaagctat acatttacga tcactgccct
    85861 tactgcctca aagcccgcat gattttcggc ctgaaaaata tccccgtcga attacatgtt
    85921 ctgctcaacg acgacgcaga aacacccacc cggatggtcg gtcaaaaaca ggttcccatt
    85981 ctgcaaaaag atgacagccg ctatatgcca gaaagcatgg atatcgttca ctatgtcgat
    86041 aaactcgacg gcaaaccgtt actgaccggc aaacgttccc ctgccattga agagtggctg
    86101 cgcaaggtca atggctacgt caacaaactg ctgttgccgc gttttgccaa atcggcattt
    86161 gatgagtttt ctactcccgc cgcgcgcaaa tatttcgtcg acaagaaaga ggccagcgcg
    86221 ggtaattttg ccgacctgct ggcccactct gacggtctga ttaagaatat cagcgatgat
    86281 ttgcgtgcgc tggacaaact gatcgtcaaa ccgaacgccg tgaatggcga actttcggaa
    86341 gatgatattc agctatttcc gctactgcgt aatctgacgc tggtagccgg aatcaactgg
    86401 ccaagccgcg ttgctaatta ccgcgataat atggcgaaac agacacaaat caatttgtta
    86461 tcatcaatgg cgatttaatc ctgacccgcc gcggcagcct gtattcgcgg gttgccgccg
    86521 ttttcagttt tttctgctgg cgagtttatc gctggtgaca catgctgcgt tgtgtcagga
    86581 tattgacgca gatttcatgt cgaggactac catgaacaag tttttatttg ccgctgcatt
    86641 gatcgtcagt ggcctgctcg ttggctgtaa tcaactcacc caatacacca tcaccgaaca
    86701 agaaattaac cagtcgcttg cgaaacataa taatttctca aaagatatcg gtttacccgg
    86761 cgtggctgac gcccatattg ttctgacaaa cttgaccagc caaattggtc gcgaagagcc
    86821 gaataaggtt accctaaccg gagacgccaa tctggacatg aactccctgt tcggtagtca
    86881 gaaagcgacc atgaaactga agctgaaagc gctgcccgtg tttgataaag aaaaaggtgc
    86941 gatcttcctg aaagagatgg aagtggtcga tgcgacggta caaccggaaa aaatgcaaac
    87001 ggtgatgcaa acgttgcttc cctatttgaa ccaggcatta cgcaattact ttaaccagca
    87061 acctgcttac gtcctgcgcg aagatggcag tcagggcgaa gcaatggcga aaaaactggc
    87121 gaaaggcatt gaagtgaagc caggcgaaat tgtcattccg tttacggatt aatgacgagg
    87181 gcgcattcgc gccctttatt tttcgtgcaa aggaaaacgt ttccgcttat cctttgtgtc
    87241 cggcaaaaac atcccttcag ccggagcata gagattaatg actgcaccat cccaggtatt
    87301 aaagatccgc cgcccagacg actggcacct tcacctccgc gatggcgaca tgttaaaaac
    87361 tgtcgtgccg tataccagcg aaatttatgg acgggctatc gtaatgccca atctggctcc
    87421 gcccgtgacc acagttgagg ctgccgtggc gtatcgccaa cgtattcttg acgccgtacc
    87481 tgccgggcac gatttcaccc cattgatgac ctgttattta acagattcgc tggatcctaa
    87541 tgagctggag cgcggattta atgaagccgt gttcaccgct gcaaaacttt acccggcaaa
    87601 cgcaaccact aactccagcc acggcgtgac gtcaattgac gcaatcatgc cggtacttga
    87661 gcgcatggaa aaaatcggta tgccgctact ggtgcatggt gaagtgacac atgcagatat
    87721 cgacattttt gatcgtgaag cgcgctttat agaaagcgtg atggaacctc tgcgccagcg
    87781 cctgactgcg ctgaaagtcg tttttgagca catcaccacc aaagatgctg ccgactatgt
    87841 ccgtgacgga aatgaacggc tggctgccac catcactccg cagcatctga tgtttaaccg
    87901 caaccatatg ctggttggtg gcgtgcgtcc gcacctgtat tgtttaccca tcctcaaacg
    87961 caatattcac caacaggcat tgcgtgaact ggtcgccagc ggttttaatc gagtattcct
    88021 cggtacggat tctgcgccac atgcacgtca tcgcaaagag agcagttgcg gctgcgcggg
    88081 ctgcttcaac gccccaaccg cgctgggcag ttacgctacc gtctttgaag agatgaatgc
    88141 tctgcagcac tttgaagcat tctgttctgt aaacggcccg cagttctatg gcttgccggt
    88201 caacgacaca ttcatcgaac tggtacgtga agagcaacag gttgctgaaa gcatcgcact
    88261 gactgatgac accctggtgc cattcctcgc cggggaaacg gtacgctggt ccgttaaaca
    88321 ataaaaatcg tagcttcctg ttgtcattag gttattttac ctgtataaat aaccagtata
    88381 ttcaacaggg ggctattatg cgaattgaag tcaccatagc gaaaacttct ccattgccag
    88441 ctggggctat tgacgccctg gctggcgaac tttcccgccg tattcagtat gcgtttcctg
    88501 ataatgaagg ccacgtatcg gtacgttatg ccgcagcgaa taatttatcg gttattggcg
    88561 caacaaaaga agataaacag cgcattagcg aaattctcca ggaaacgtgg gaaagcgccg
    88621 atgactggtt tgtcagcgaa taatatgcag tgatttttgt ttttgccgga tcgccccggc
    88681 ttttttattt tacttacgaa ttttgagtac aaaatttcta cacttcctca aaataattgc
    88741 acaatatgct aaaaaaatat gaatgacatg ctgttttatt gttcatataa atcttaaaag
    88801 cagaaattta tctgttcccc cctttaaaaa ttcggtgaat acccttactt attggtatac
    88861 tgaagttgct tcagataaaa acgcattgaa cctcgaataa cgttgtctag taacacgaat
    88921 tagggggcca tgatggaaaa aaataatgaa gtcattcaga ctcatccgct cgtagggtgg
    88981 gacatcagca ccgttgatag ctatgatgcg ctgatgttgc gtttgcacta ccagacccca
    89041 aataagtccg agcaggaagg gactgaagtt ggtcagacgc tctggttaac cactgatgtt
    89101 gccagacaat ttatttcgat attagaagca ggaatcgcca aaattgaatc cggtgatttc
    89161 ccggtaaacg agtatcggcg tcattaacat gacatgacca tccacccaaa aggcacctca
    89221 ccggtgcctt taccatttct gggttgtata tctgcattca gttaattact ctgcttaaac
    89281 catgagagca gagagaaaaa aatgaaatac gatctcatca ttattggcag cggttccgta
    89341 ggcgctgccg ccgggtatta tgcaacccgc gccggtttaa acgtgctaat gaccgacgcc
    89401 catatgccac cgcatcaaca cggcagccac cacggcgata cgcgattaat tcgccatgct
    89461 tatggtgaag gcgaaaagta tgtcccgttg gtcctccgcg cgcaaacgct gtgggatgaa
    89521 ctttcccgcc acaacgaaga tgatcccatt tttgtacgct ctggtgtcat taacctcggc
    89581 ccggctgact ccgcatttct cgccaacgtc gcccacagcg ccgaacagtg gcaactcaac
    89641 gttgaaaagc tcgatgcgca agggattatg gcccgctggc cagaaatacg cgtcccggac
    89701 aactacatcg gcttatttga gactgattcc ggttttttgc gcagcgaact ggcgattaaa
    89761 acctggatcc aactggcgaa ggaagcgggc tgtgcgcaac tgttcaactg cccggtcacc
    89821 gcaattcgtc atgacgatga tggcgtaact attgaaacgg ttgacggtga gtatcaggcg
    89881 aaaaaagcga ttgtctgcgc gggaacatgg gtaaaagacc tgctcccgga gctgcctgtc
    89941 cagcccgtac gtaaagtatt tgcctggtat caggccgatg gccgctatag cgtgaagaat
    90001 aaattcccgg cgtttaccgg tgaactgccc aatggcgatc aatattatgg ttttccggca
    90061 gaaaacgacg cgttgaagat tggcaaacat aacggaggcc aggttatcca ttcagcggat
    90121 gaacgtgttc cgtttgcgga agtggccagt gatgggtcag aagccttccc gttcttgcgc
    90181 aatgtattgc cgggtatcgg ttgctgcctg tacggcgctg cctgcaccta tgataattcg
    90241 cctgacgaag attttattat cgatacccta cccggccacg ataatacact gctcattacc
    90301 ggcctgagtg ggcacggttt taaatttgcg tcagttttag gggaaatagc tgccgatttt
    90361 gcgcaagacc aaaaaagcga ttttgatttg acgccattca ggctttcccg cttccaataa
    90421 tcatcattac ggcctccaga atgaggccgt ttttacagga gtatttaatg cgtcggctgt
    90481 tgcactatct catcaataat attcgcgagc atctgatgct ttatcttttt ctctggggat
    90541 tgttggcaat tatggatctg atctatgtgt tttattttta gttttccgca ctgacataaa
    90601 ctgacaaata tttaatctcc ctttcattta gcgtgaaaaa ttgaatgaga ttattcctgc
    90661 ttctacagca ctcaccagac atctctgatg ctgaacccac taaacttatg aagattcttt
    90721 cattattaat tcaaaattat tgaaataaaa ataaatatca ttcaatttgt tatttttgtg
    90781 ttgcaattca ttctccgaca cgccattttc tttctctcgc aaattcgtat tggatgcact
    90841 tatgtcattc acaaataccc ctgaacgcta tggtgttata tctgcggcct tccattggct
    90901 aagcgcaatt attgtctacg gcatgtttgc tttaggtctg tggatggtca cgctcagtta
    90961 ttacgatggc tggtatcaca aagcacccga actgcataaa agcatcggta ttttgttaat
    91021 gatggggctg gttattcgcg ttctgtggcg tgtcatatct ccaccgccgg gtccgctgcc
    91081 gagttattcg cccatgactc gtcttgccgc aaaagctgga catctcgcat tgtgcctatt
    91141 gctgttcgcc atcggcatca gcggctatct gatctcaact gccgatggta aaccgatcag
    91201 cgtttttggc tggtttgacg tccccgcgac ccttgccgac gctggcgcac aggcagactt
    91261 tgccggtgcc ctgcattttt ggctcgcgtg gagcgtcgta gtactgtctg ttatgcacgg
    91321 atttatggcc ctgaagcatc atttcatcga taaagacgac actctgaagc gcatgctggg
    91381 aaagtcgtca tctgactatg gagtataaaa aatgaaaaaa agcctgcttg gtttaacctt
    91441 cgcgtccctg atgttctctg ccggttcagc ggttgccgcc gattacaaaa ttgacaaaga
    91501 aggtcagcac gcctttgtta acttccgcat acagcatctt ggctatagct ggttatatgg
    91561 cacctttaaa gatttcgacg gtacttttac ctttgatgaa aaaaatccgg ctgccgataa
    91621 agtgaatgtg acaattaaca ccaccagcgt cgatactaac cacgccgaac gcgataaaca
    91681 tcttcgcagt gcggattttc tcaataccac aaaatatcca caggcaacat tcacctccac
    91741 cagcgtgaag aaagacggtg acgaactgga tattaccggc gatctgacgc tgaatggcgt
    91801 aaccaaaccc gtcacgctgg aagcgaaatt aattggtcag ggcgacgacc catggggcgg
    91861 taaacgtgca ggctttgagg ccgaaggcaa aattaagctc aaagacttca atatcaagac
    91921 agatttaggt ccagcttctc aggaagtgga tctgattatt tcagtggaag gcgtacagca
    91981 gaagtaagag tgaaatgttg ccggatggca cgcatccggc aatgatattt attctgttgg
    92041 atcaggaata cccagtgttg tattcagacg tccacgagac ttattaaaga tcttattgcc
    92101 attttcacgt cctgcccgac ggcgtcgctg ttcttctggc ggtaacgcgc tttcttcgca
    92161 gcaaatctca ctacaacaac ctttgtattt ttccgcgcat actggacact gaataaacag
    92221 caggtggcag ccatcatttt tacagttggt atggctgtcg cacggcgcgc cgcactggtg
    92281 gcaatgcgcg ataatctcat ccgatatacg ttcgcccatc cgctcgtcaa aaacaaaatt
    92341 tttgccaata aaacgcaccg gcaagccctg ctcgcgcgcc ttacgggcgt attcaataat
    92401 tccgccctcg atatgccaga ctttgttgaa tccgttatgt ttcatccagg cactggcctt
    92461 ttcacaacga atgccgccgg tgcagtacat gacgattttt ttatctttat gtgcctgcat
    92521 catctcgact gcttttggca gctgctcacg gaaggtatct gccggaattt cgagcgcgtt
    92581 ttcaaagtgc cccacttcat actcatagtg gttacgcatg tcgataaata gtgcatcggg
    92641 atcgtcaagc atggcgttca cttccgccgc ttgcagatac tcaccaacat tgctggcatc
    92701 aaagtgagga tcgtcaatac cgtcggcaac aatgcgatcg cgtaccttca tgcgcagtac
    92761 ccagaaggat ttcccgtcat catccaacgc gatattcagg cgtaaaccct ctaaagccgg
    92821 gtcgaaggca tagagctgcg cgcgaaatgt ttcaacattg ctcgcaggta cgctgatttg
    92881 cgcgttaatg ccctcatgcg ccagatacac tcgcccaaaa acattcagcg cggtaaacag
    92941 ctgatataaa gcgtcacggg tcgccttagg atcggcgatg tggaaatact tgtaaaacga
    93001 aatggtggtt cgcggttcgc tctcagccaa cattttggct tttaacgcgt cgttggaaat
    93061 gcggttgtgt aacactggca tggtgtacgg ttcctgcgag atgggaaagt aaaaatccgc
    93121 ggcatgatat agcaattatc gataattaac atccacatat tttacgctac atttgcgcat
    93181 taaaaattat ttgttattta caagcgcggc aatttcgccc agtcttcagc cacaaatttt
    93241 ggttgcgggc gaaaaaatgc gacaatacat acaattgccc gtataggttg aaaaacagga
    93301 ttgatatgac gaatctaccc aagttctcca ccgcactgct tcatccgcgt tattggttaa
    93361 cctggttggg tattggcgta ctttggttgg tcgtgcaatt gccctacccg attatctacc
    93421 gcctcggttg tggattagga aaactggcgt tacgttttat gaaacgacgc gcaaaaattg
    93481 tgcatcgcaa cctggaactg tgcttcccgg aaatgagcga acaagaacgc cgtaaaatgg
    93541 tggtgaagaa tttcgaatcc gttggcatgg gcctgatgga aaccggtatg gcgtggttct
    93601 ggccggaccg ccgaatcgcc cgctggacgg aagtgatcgg catggaacac attcgtgacg
    93661 tgcaggcgca aaaacgcggc atcctgttag ttggcatcca ttttctgaca ctggagctgg
    93721 gcgcgcggca gtttggtatg caggaaccgg ggattggcgt ttaccgcccg aacgataatc
    93781 cactgattga ctggctacaa acctggggcc gtttgcgctc aaataaatcg atgctcgacc
    93841 gcaaagattt aaaaggcatg attaaagccc tgaaaaaagg cgaagtggtc tggtacgcac
    93901 cagatcatga ttacggcccg cgctcaagcg ttttcgtccc gttatttgcc gttgagcagg
    93961 ctgcgaccac gaccggaacc tggatgctgg cacggatgtc cggcgcatgt ctggtgccct
    94021 tcgttccacg ccgtaagcca gatggcaaag ggtatcaatt gattatgctg ccaccagagt
    94081 gttctccgcc actggatgat gccgaaacta ccgccgcgtg gatgaacaaa gtggtcgaaa
    94141 aatgcatcat gatggcacca gagcagtata tgtggttaca ccgtcgcttt aaaacacgcc
    94201 cggaaggcgt tccttcacgc tattaaatct cccatgccgg atgcttcaga atggcatccg
    94261 gcattaccac agcaaatccc cctgatttag cgataaaagc tctctggatt gcgccccctg
    94321 gaagtcgggc gcataattag tgtgtttatc ttttcttctt atgttcaccg cgcctggcgc
    94381 accaacagcg gattgctatg tcaccctgtg aaaatgacac ccctataaac tggaaacgaa
    94441 acctgatcgt cgcctggcta ggctgttttc ttaccggggc cgccttcagt ctggtaatgc
    94501 ccttcttacc cctctacgtt gagcagcttg gtgttaccgg ccactccgcc ctgaatatgt
    94561 ggtccggtat tgtcttcagt ataatgggtt cctaaacaca tcattttagg tattgaatat
    94621 gagacgggca atcagctaca tcagattttc aagtgaacga caactgaaag gggactctgt
    94681 acgccgacaa tctaagctgg ttactgactg gcttgataag aaccctgagt tttacttgga
    94741 ttcatccttg agctttaaag atttggggaa aagtgctttt agtggtaagc acctgaaagg
    94801 tggtctaggt gactttttaa ccgctatcga gaaagggctt gttaaagctg gtgatacgct
    94861 actaatcgag agtttagacc gtctgtcacg tcaggatatt gatattgctt ctgagctatt
    94921 acgccgtatc ttaagggctg gtgtagatgt agtcacactg agcgatggag aacattacac
    94981 acgagaaagt ttaaaagacc cgttagccct gattaaaagc attctgataa tgcagcgggc
    95041 gcatgaggaa agtttgagaa agtctgagcg tgtccaagct gcatggaacc gtaaaaaaga
    95101 actcatctcc gaaggtatca aggtatctag gcgttgtcct gcatggttaa ggctgaatga
    95161 tgaccgtcga acattcacaa tcattcctga taaagtggaa gttgttaaac gagcatttga
    95221 cctgagatta cagggtttaa gcttttgggc tatcaccaga actttaaacg atgaaggtca
    95281 cttgtcattg aatcaataca cacccaaaca aaaggggtgg tctgatactg ctgtaaagaa
    95341 actgctcaga aatcgtgctg tgattggatg tttcacacct gctggacgtg aagaagttca
    95401 gggttactac cccgcaataa tcagtgagtc actattctat cgagtacagc aactgaacac
    95461 agggcaatat ggtagggctt ctgtatcaag taatccgcta tcagtcaact tgttcagggg
    95521 tatcatcaaa tgtgaggtgt actg
//
LOCUS       DAJTRD010000003        75450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTRD010000003 DAJTRD010000000
VERSION     DAJTRD010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.003"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(435..641)
                     /locus_tag="OO315_000238"
     CDS             complement(435..641)
                     /locus_tag="OO315_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5889296.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            954..1043
                     /gene="kdpF"
                     /locus_tag="OO315_000239"
     CDS             954..1043
                     /gene="kdpF"
                     /locus_tag="OO315_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5889297.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1043..2716
                     /gene="kdpA"
                     /locus_tag="OO315_000240"
     CDS             1043..2716
                     /gene="kdpA"
                     /locus_tag="OO315_000240"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5889298.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2739..4786
                     /gene="kdpB"
                     /locus_tag="OO315_000241"
                     /pseudo
     CDS             2739..4786
                     /gene="kdpB"
                     /locus_tag="OO315_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4795..5367
                     /gene="kdpC"
                     /locus_tag="OO315_000242"
     CDS             4795..5367
                     /gene="kdpC"
                     /locus_tag="OO315_000242"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5889299.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5360..8040
                     /gene="kdpD"
                     /locus_tag="OO315_000243"
                     /pseudo
     CDS             5360..8040
                     /gene="kdpD"
                     /locus_tag="OO315_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8037..8722
                     /gene="kdpE"
                     /locus_tag="OO315_000244"
                     /pseudo
     CDS             8037..8722
                     /gene="kdpE"
                     /locus_tag="OO315_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8997..9101
                     /locus_tag="OO315_000245"
     CDS             8997..9101
                     /locus_tag="OO315_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5889300.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9412..11609
                     /gene="speF"
                     /locus_tag="OO315_000246"
                     /pseudo
     CDS             9412..11609
                     /gene="speF"
                     /locus_tag="OO315_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11606..12925
                     /gene="potE"
                     /locus_tag="OO315_000247"
     CDS             11606..12925
                     /gene="potE"
                     /locus_tag="OO315_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5889301.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMAPAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="OO315_000248"
     CDS             complement(12981..14621)
                     /gene="pgm"
                     /locus_tag="OO315_000248"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5889302.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLTDI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="OO315_000249"
     CDS             complement(14647..15192)
                     /gene="seqA"
                     /locus_tag="OO315_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5889303.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15377..16141
                     /gene="ybfF"
                     /locus_tag="OO315_000250"
     CDS             15377..16141
                     /gene="ybfF"
                     /locus_tag="OO315_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5889304.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16281..16574
                     /gene="ybfE"
                     /locus_tag="OO315_000251"
     CDS             16281..16574
                     /gene="ybfE"
                     /locus_tag="OO315_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5889305.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16714..17244
                     /gene="fldA"
                     /locus_tag="OO315_000252"
     CDS             16714..17244
                     /gene="fldA"
                     /locus_tag="OO315_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5889306.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17454..17540
                     /gene="uof"
                     /locus_tag="OO315_000253"
     CDS             17454..17540
                     /gene="uof"
                     /locus_tag="OO315_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5889307.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17533..17979
                     /gene="fur"
                     /locus_tag="OO315_000254"
     CDS             17533..17979
                     /gene="fur"
                     /locus_tag="OO315_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5889308.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18063..18389)
                     /locus_tag="OO315_000255"
     CDS             complement(18063..18389)
                     /locus_tag="OO315_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889309.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="OO315_000256"
     CDS             complement(18426..19832)
                     /gene="chiP"
                     /locus_tag="OO315_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5889310.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20411..22075)
                     /gene="glnS"
                     /locus_tag="OO315_000257"
     CDS             complement(20411..22075)
                     /gene="glnS"
                     /locus_tag="OO315_000257"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5889311.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22278..24224)
                     /gene="nagE"
                     /locus_tag="OO315_000258"
     CDS             complement(22278..24224)
                     /gene="nagE"
                     /locus_tag="OO315_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5889312.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24557..25357
                     /gene="nagB"
                     /locus_tag="OO315_000259"
     CDS             24557..25357
                     /gene="nagB"
                     /locus_tag="OO315_000259"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5889313.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25417..26565
                     /gene="nagA"
                     /locus_tag="OO315_000260"
     CDS             25417..26565
                     /gene="nagA"
                     /locus_tag="OO315_000260"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5889314.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26574..27794
                     /gene="nagC"
                     /locus_tag="OO315_000261"
                     /gene_synonym="nagR"
     CDS             26574..27794
                     /gene="nagC"
                     /locus_tag="OO315_000261"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5889315.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27842..28594
                     /gene="nagD"
                     /locus_tag="OO315_000262"
                     /pseudo
     CDS             27842..28594
                     /gene="nagD"
                     /locus_tag="OO315_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28991..30655
                     /gene="asnB"
                     /locus_tag="OO315_000263"
     CDS             28991..30655
                     /gene="asnB"
                     /locus_tag="OO315_000263"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5889316.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31034..31110
                     /locus_tag="OO315_000264"
     tRNA            31034..31110
                     /locus_tag="OO315_000264"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31068..31070,aa:Met,seq:cat)
     gene            31119..31203
                     /locus_tag="OO315_000265"
     tRNA            31119..31203
                     /locus_tag="OO315_000265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31153..31155,aa:Leu,seq:tag)
     gene            31227..31301
                     /locus_tag="OO315_000266"
     tRNA            31227..31301
                     /locus_tag="OO315_000266"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31259..31261,aa:Gln,seq:ttg)
     gene            31336..31410
                     /locus_tag="OO315_000267"
     tRNA            31336..31410
                     /locus_tag="OO315_000267"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31368..31370,aa:Gln,seq:ttg)
     gene            31426..31502
                     /locus_tag="OO315_000268"
     tRNA            31426..31502
                     /locus_tag="OO315_000268"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31460..31462,aa:Met,seq:cat)
     gene            31551..31625
                     /locus_tag="OO315_000269"
     tRNA            31551..31625
                     /locus_tag="OO315_000269"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31583..31585,aa:Gln,seq:ctg)
     gene            31663..31737
                     /locus_tag="OO315_000270"
     tRNA            31663..31737
                     /locus_tag="OO315_000270"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31695..31697,aa:Gln,seq:ctg)
     gene            complement(31891..33066)
                     /gene="ubiF"
                     /locus_tag="OO315_000271"
     CDS             complement(31891..33066)
                     /gene="ubiF"
                     /locus_tag="OO315_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5889317.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33212..34636
                     /gene="miaB"
                     /locus_tag="OO315_000272"
     CDS             33212..34636
                     /gene="miaB"
                     /locus_tag="OO315_000272"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5889318.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDASHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34789..35829
                     /locus_tag="OO315_000273"
     CDS             34789..35829
                     /locus_tag="OO315_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5889319.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35826..36293
                     /gene="ybeY"
                     /locus_tag="OO315_000274"
     CDS             35826..36293
                     /gene="ybeY"
                     /locus_tag="OO315_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5889320.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36383..37261
                     /gene="corC"
                     /locus_tag="OO315_000275"
     CDS             36383..37261
                     /gene="corC"
                     /locus_tag="OO315_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5889321.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37286..38824
                     /gene="lnt"
                     /locus_tag="OO315_000276"
     CDS             37286..38824
                     /gene="lnt"
                     /locus_tag="OO315_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5889322.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39221..40129
                     /gene="gltI"
                     /locus_tag="OO315_000277"
     CDS             39221..40129
                     /gene="gltI"
                     /locus_tag="OO315_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5889323.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40299..41039
                     /gene="gltJ"
                     /locus_tag="OO315_000278"
     CDS             40299..41039
                     /gene="gltJ"
                     /locus_tag="OO315_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5889324.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            41039..41713
                     /gene="gltK"
                     /locus_tag="OO315_000279"
     CDS             41039..41713
                     /gene="gltK"
                     /locus_tag="OO315_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5889325.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41713..42438
                     /gene="gltL"
                     /locus_tag="OO315_000280"
     CDS             41713..42438
                     /gene="gltL"
                     /locus_tag="OO315_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5889326.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42556..43491
                     /gene="rihA"
                     /locus_tag="OO315_000281"
     CDS             42556..43491
                     /gene="rihA"
                     /locus_tag="OO315_000281"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5889327.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43575..45245
                     /gene="hscC"
                     /locus_tag="OO315_000282"
                     /pseudo
     CDS             43575..45245
                     /gene="hscC"
                     /locus_tag="OO315_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45305..46756)
                     /gene="djlC"
                     /locus_tag="OO315_000283"
     CDS             complement(45305..46756)
                     /gene="djlC"
                     /locus_tag="OO315_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5889328.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46753..47460)
                     /locus_tag="OO315_000284"
     CDS             complement(46753..47460)
                     /locus_tag="OO315_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5889329.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47562..48116
                     /locus_tag="OO315_000285"
     CDS             47562..48116
                     /locus_tag="OO315_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5889330.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48126..49553)
                     /locus_tag="OO315_000286"
     CDS             complement(48126..49553)
                     /locus_tag="OO315_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5889331.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49550..50257)
                     /locus_tag="OO315_000287"
     CDS             complement(49550..50257)
                     /locus_tag="OO315_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5889332.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            50421..51398
                     /locus_tag="OO315_000288"
     CDS             50421..51398
                     /locus_tag="OO315_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5889333.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51468..51950)
                     /locus_tag="OO315_000289"
     CDS             complement(51468..51950)
                     /locus_tag="OO315_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5889334.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52185..54767
                     /gene="leuS"
                     /locus_tag="OO315_000290"
     CDS             52185..54767
                     /gene="leuS"
                     /locus_tag="OO315_000290"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5889335.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54782..55363
                     /gene="lptE"
                     /locus_tag="OO315_000291"
     CDS             54782..55363
                     /gene="lptE"
                     /locus_tag="OO315_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5889336.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55363..56394
                     /gene="holA"
                     /locus_tag="OO315_000292"
     CDS             55363..56394
                     /gene="holA"
                     /locus_tag="OO315_000292"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5889337.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56396..57037
                     /gene="nadD"
                     /locus_tag="OO315_000293"
     CDS             56396..57037
                     /gene="nadD"
                     /locus_tag="OO315_000293"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5889338.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            57061..57672
                     /locus_tag="OO315_000294"
     CDS             57061..57672
                     /locus_tag="OO315_000294"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5889339.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57932..58249
                     /gene="rsfS"
                     /locus_tag="OO315_000295"
                     /gene_synonym="rsfA"
     CDS             57932..58249
                     /gene="rsfS"
                     /locus_tag="OO315_000295"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5889340.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58253..58720
                     /gene="rlmH"
                     /locus_tag="OO315_000296"
     CDS             58253..58720
                     /gene="rlmH"
                     /locus_tag="OO315_000296"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5889341.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58751..60652
                     /gene="mrdA"
                     /locus_tag="OO315_000297"
     CDS             58751..60652
                     /gene="mrdA"
                     /locus_tag="OO315_000297"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5889342.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60655..61767
                     /gene="mrdB"
                     /locus_tag="OO315_000298"
                     /gene_synonym="rodA"
     CDS             60655..61767
                     /gene="mrdB"
                     /locus_tag="OO315_000298"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5889343.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61778..62866
                     /gene="rlpA"
                     /locus_tag="OO315_000299"
     CDS             61778..62866
                     /gene="rlpA"
                     /locus_tag="OO315_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5889344.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            63005..64216
                     /gene="dacA"
                     /locus_tag="OO315_000300"
     CDS             63005..64216
                     /gene="dacA"
                     /locus_tag="OO315_000300"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5889345.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64327..64590
                     /locus_tag="OO315_000301"
     CDS             64327..64590
                     /locus_tag="OO315_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5889346.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64691..65332
                     /gene="lipB"
                     /locus_tag="OO315_000302"
     CDS             64691..65332
                     /gene="lipB"
                     /locus_tag="OO315_000302"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5889347.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65591..66544
                     /locus_tag="OO315_000303"
     CDS             65591..66544
                     /locus_tag="OO315_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5889348.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66753..67718
                     /gene="lipA"
                     /locus_tag="OO315_000304"
     CDS             66753..67718
                     /gene="lipA"
                     /locus_tag="OO315_000304"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5889349.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67819..68022)
                     /gene="tatE"
                     /locus_tag="OO315_000305"
     CDS             complement(67819..68022)
                     /gene="tatE"
                     /locus_tag="OO315_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5889350.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68151..68939)
                     /locus_tag="OO315_000306"
     CDS             complement(68151..68939)
                     /locus_tag="OO315_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5889351.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            69032..69415
                     /gene="crcB"
                     /locus_tag="OO315_000307"
     CDS             69032..69415
                     /gene="crcB"
                     /locus_tag="OO315_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5889352.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69469..69678)
                     /gene="cspE"
                     /locus_tag="OO315_000308"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69469..69678)
                     /gene="cspE"
                     /locus_tag="OO315_000308"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5889353.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69853..70413)
                     /gene="pagP"
                     /locus_tag="OO315_000309"
     CDS             complement(69853..70413)
                     /gene="pagP"
                     /locus_tag="OO315_000309"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5889354.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            71002..72387
                     /gene="dcuC"
                     /locus_tag="OO315_000310"
     CDS             71002..72387
                     /gene="dcuC"
                     /locus_tag="OO315_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5889355.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72428..72769)
                     /locus_tag="OO315_000311"
                     /pseudo
     CDS             complement(72428..72769)
                     /locus_tag="OO315_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(73107..73616)
                     /locus_tag="OO315_000312"
     CDS             complement(73107..73616)
                     /locus_tag="OO315_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5889356.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73779..74024
                     /locus_tag="OO315_000313"
     CDS             73779..74024
                     /locus_tag="OO315_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5889357.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73999..>75450
                     /locus_tag="OO315_000314"
     CDS             73999..>75450
                     /locus_tag="OO315_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5889358.1"
                     /translation="MREWISAIECMGLSGLPGSLMGVHKRAQREGWLKRRREGIRGVA
                     YEYHISSLPNIARAEVLLQQGKFKTSQGCFEIARPTLEAHDYDREALWSKWDKASDSQ
                     RSLAEKWLPSIQATDEMLNQGISTKTAFATVAGHYQVSASTLRDKYYQVQKFAKPDWA
                     AALVDGRGASRRNVHKSEFDEDAWQFLIADYLRPEKPAFRKCYERLELAAREHGWSIP
                     SRATAFRRIQQLDEAMVVACREGEHALMHLIPAQQRTVEHLDAMQWINGDGYLHNVFV
                     RWFNGDVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRYSIPEDFHITI
                     DNTRGAANKWLTGGAPNRYRFKVKEDDPKGLFLLMGAKMHWTSVVAGKGWGQAKPVER
                     AFGVGGLEEYVDKHPALAGAYTGPNPQAKPDNYGDHAVDAELFLKTFAEGVAMFNART
                     GRETEMCGGKLSFDDVFEREYARTIVRKPTEEQK"
BASE COUNT        18562 a        18959 c        19160 g        18769 t
ORIGIN      
        1 cataacatct tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag
       61 tatatattac tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc
      121 ggaatttatt tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga
      181 agtttcacga aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt
      241 cgcttagagc gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg
      301 aaacgtgatg ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa
      361 ttatcatgga agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt
      421 aagcgcgcag gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc
      481 ggtttatcgc tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct
      541 ttccagaaaa gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt
      601 aaaaagataa gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt
      661 atctacccgg tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc
      721 cttttgtgta attttactac tcatccgacc acttattttt gcttattgat ggtttattta
      781 cattcacact gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca
      841 tggcctttgc catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt
      901 gcagccagaa ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg
      961 caggcgtgat agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc
     1021 tgatcaatgc ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt
     1081 actggtatta atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga
     1141 cattcctctt ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc
     1201 tgaccatgag atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg
     1261 gctggcggtg ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca
     1321 gttgccgggg ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac
     1381 caactggcaa tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt
     1441 aacggtacaa aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg
     1501 tgcgtttact cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat
     1561 cacgttatgg gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg
     1621 tgcgcagcaa aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct
     1681 gttacccatg ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg
     1741 tggcttcttt aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt
     1801 cgtgcagatg ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt
     1861 gacgggcgat cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat
     1921 ctgcgtaggc gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg
     1981 cgcagacagc agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag
     2041 cctgtttgcg gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc
     2101 gtttaccgct ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt
     2161 cggcggtgtc ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat
     2221 tgccgggctg atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga
     2281 gatgaaactg actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc
     2341 gttggcgatg atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt
     2401 tagcgaagtg ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg
     2461 attaagcgcc aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg
     2521 cttcggggtg attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca
     2581 acccgccagc tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg
     2641 caccgtgttg ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc
     2701 ggaatatctc tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc
     2761 tattcgaacc aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc
     2821 aggcgcaatg gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct
     2881 gtattagcat cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa
     2941 ttagcggctg gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag
     3001 gccgcagtaa agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca
     3061 agctgcgtga gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta
     3121 aaggtgacat cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg
     3181 aaggcggcgc atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg
     3241 aatccggcgg cgattttgcc tccgtcaccg gcggcacgcg tattctttct gactggctgg
     3301 tgattgagtg tagcgttaac cccggtgaga catttctgga tcggatgatc gcgatggtgg
     3361 aaggcgcaca gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc
     3421 tgaccatcgt ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta
     3481 atgccgtgag tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg
     3541 gtggcctgtt gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga
     3601 ttgccaccag cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata
     3661 aaaccggcac catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg
     3721 tggatgaaaa aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg
     3781 aaggtcgcag tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc
     3841 agtcgctcca tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca
     3901 tcgacaaccg catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta
     3961 acggcggtca cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag
     4021 ccacgctgct ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata
     4081 tcgtcaaagg cggtattaaa gagcgcttcg cccggctgcg caaatgggca ttaaaacggt
     4141 gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga
     4201 tgattttctc gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc
     4261 ggaaggtcgt ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca
     4321 ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat
     4381 ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat
     4441 gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt
     4501 cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat
     4561 gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat
     4621 cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc
     4681 catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg
     4741 tatcaaagtc attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt
     4801 ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac
     4861 ccgctgctga ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg
     4921 attcgtgaag gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac
     4981 ggctattttc atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct
     5041 ggcgggagca atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt
     5101 gccgcattac gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg
     5161 gcgtcggcaa gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca
     5221 cgcatcgcaa aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac
     5281 agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat
     5341 ctggcactgg ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct
     5401 ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg
     5461 cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga
     5521 tattgtggtt ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg
     5581 gctggctgtt ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga
     5641 tctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag
     5701 taatgcgcca ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc
     5761 tggcattgat gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt
     5821 cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc
     5881 cgacgacgtg gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg
     5941 caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa
     6001 tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat
     6061 gcgcgcctgg cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct
     6121 tttatgcatc ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg
     6181 tcacggctgg ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta
     6241 ccggagaaaa aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg
     6301 gagacggcaa cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa
     6361 cataatctcg gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa
     6421 acatttgctg accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt
     6481 gatgaaccac ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag
     6541 tggcgggtac agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta
     6601 attgccatgc agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt
     6661 ggcgtggtgg tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat
     6721 gtggtgagtt tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg
     6781 caatatctgc tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact
     6841 gctggtgtgc gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta
     6901 tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc
     6961 gaacaattta ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac
     7021 ggtaaattgc agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg
     7081 cagtggagtt ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta
     7141 ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag
     7201 ccggggaatc tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg
     7261 ctgttagtcg ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg
     7321 atggcaagcg aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta
     7381 cgcacgccgc ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc
     7441 gaaggatcac cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc
     7501 cgactggtga ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag
     7561 aaagagtggt taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt
     7621 ttattgtcgc ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg
     7681 ccactctttg aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg
     7741 caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg
     7801 gataacggcc ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc
     7861 gggaataaag agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt
     7921 gtggatgtac acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt
     7981 cgtgttacac ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga
     8041 caaacgttct gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg
     8101 agggcgacgg gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg
     8161 caacccgtaa gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg
     8221 agtttatccg cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca
     8281 gcgaagagag cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc
     8341 cgtttggtat tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca
     8401 ctccgccacc gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc
     8461 cgcccgcgtg attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct
     8521 gctggcggtg ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca
     8581 ggtgtggggg ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct
     8641 gcgacaaaaa ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat
     8701 tgggtatcgg tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt
     8761 tattattaaa cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat
     8821 aaaacacgaa attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa
     8881 ttaatgattt attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc
     8941 catttcatgg atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg
     9001 aaaataataa ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc
     9061 atcgtaatag ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac
     9121 cccaacgtac aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg
     9181 ctcatatact gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg
     9241 aggtatgacg agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca
     9301 aactactgat ttatttatca gcggtggagc tttgcttttt ttccgacgtg atcgatttct
     9361 cctttgagaa attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa
     9421 ttaaaaattg ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg
     9481 aatattgacg aaagtaatta tattgacgtt gccgccatta ttttatcagt caatgatgtt
     9541 gaacgtggaa aactcgataa aatagacgct actggctatg gcattcctgt ttttattgca
     9601 acggaaaatg aagaacgtgt accagcagaa tatctgccac gtatttctgg tgtcttcgag
     9661 cattgcgaat cacggaaaga gttttatgga cgtcagttag aaaccgctgc tagccattat
     9721 gaaacgcaac tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggcaat
     9781 agcgcttttg actgcccggg tcatcaggga ggtgagtttt tccgtcgtca tccggcaggg
     9841 aatcagtccg tggagtactt cggagaaacg ctgttccgtt ccgacttatg caacgcagac
     9901 gtagcaatgg gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg
     9961 gcaaaagtgt ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac
    10021 aaagtggttt taaacgccct gctaacaccg ggtgatctgg tgctgtttga tcgcaataac
    10081 cacaaatcta accaccacgg agcgttgcta caggctggtg caacaccggt ttatctggaa
    10141 acggcacgta acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt
    10201 tacctgcgtg agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc
    10261 cgcctcgctg tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg
    10321 gtggataaaa ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat
    10381 gagcaattta tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac
    10441 gatccgggta ttctagttac gcaatccgta cataaacagc aggctggttt ttctcaaact
    10501 tcacaaatcc ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa
    10561 cgcatgaaca acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc
    10621 gcactggata tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat
    10681 tgtgtggtaa atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt
    10741 ccgttcgtac ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt
    10801 gcggttgatc tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc
    10861 tatgcagaga atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt
    10921 gatgcacgta acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc
    10981 ctgcgcgaaa atggcgtagt gccggaaaaa tgcgatctta acgccatcct cttcctgctg
    11041 actccggcag aagatatggc caaacttcag caacttgtgc cctgctggta cgcttcgaaa
    11101 aactgcttga gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg
    11161 aagagcgcta cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg
    11221 cccgccacaa cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg
    11281 tcagcatgaa tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc
    11341 gtctaccgga tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg
    11401 tgctgtgtgt tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc
    11461 tggaagaagg gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg
    11521 aagaacatga tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa
    11581 gcaccctgtt gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt
    11641 tcagttaacc atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac
    11701 aaagcttgcc gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc
    11761 aatggcaccg gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat
    11821 gggcggttat gccgagtatg ctttcggtaa atccggtaac tttattgcga actataccta
    11881 cggcgtctca ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac
    11941 cgaactgctc ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct
    12001 gtggatttgt accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat
    12061 taccgtgtgg gggggcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt
    12121 tagcccgacg ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt
    12181 aggttcttcc atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa
    12241 tactgatgta gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt
    12301 aggtgcggcg gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat
    12361 ggagctggca aattcaacgg caccatttgg cctggccttc gcacagatgt ttacaccaga
    12421 agtgggtaaa gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg
    12481 gcagttcacc attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat
    12541 tttctcccgt gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat
    12601 ccagagcggg ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct
    12661 ggttaacctg gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt
    12721 cattattcag aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc
    12781 ttttgttggc gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct
    12841 gtacggttcc atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt
    12901 tgaactgaaa aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc
    12961 tcttttttta ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct
    13021 tctttctcaa tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat
    13081 gcgtcttccg tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga
    13141 ccgccaatag aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacct
    13201 gccagggtgc tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg
    13261 gaagtcgcag ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg
    13321 ttgtagtgtt cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac
    13381 ataatgatgc cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc
    13441 cctgcgctct cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat
    13501 ttgaaaccta ccggtacttc taccagctta cggcccaagt cattgaccac acggtcgatc
    13561 atcgcagaag aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc
    13621 tggaacaggt agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga
    13681 gtgacgatac cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca
    13741 cgcagtgcca gcagacccgc catcgcacac tcggaggagc agtccatacg gatcgcaccg
    13801 tctttatcga ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg
    13861 ttgaggttgt aatactcgcc aatgcgcttc cagtattcga taccggaacc gcccaacgga
    13921 tcaacgccca gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggtcagc
    13981 ccttccacga acggctgcac cagatcctgc tctttcacat agccggatgc catcgcttcg
    14041 tcgagggaga tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggccttgtct
    14101 tccaccactt tggtgacgtt agtatcagcc gggccaccat ttggcggatt gtacttgata
    14161 ccaccatctt ccggcgggtt atgggacggt gtaatcacga taccgtctgc cagcgggcca
    14221 ccttttttat tgtgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg
    14281 ttttcctgca caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac
    14341 gcaggctcgg acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt
    14401 ttcgcacgtt cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg
    14461 tggcgcgctg cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc
    14521 cctgcttctg gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca
    14581 ctctgttgtg caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc
    14641 aacgttttag atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg
    14701 catgatgtgt tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata
    14761 cggcgtgcct ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc
    14821 atctgccgca aagtacacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc
    14881 ctgggcgtca agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt
    14941 ttgctctgcg tattcgtccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat
    15001 cgttttgacc ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac
    15061 cggagcagca ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc
    15121 gctctcgccg atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc
    15181 aatcgttttc atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caagagtata
    15241 tgaataaatc ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac
    15301 cttatttgtt gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga
    15361 aaaaaagaac tttgccatga aattaaatat ccgcgcgcaa actgcacaaa accagcacaa
    15421 taattctccc atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact
    15481 ggctcgcgat ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct
    15541 ttcaccgaga gagccggtaa tgaattaccc ggcgatggcg caggatcttg ttgataccct
    15601 ggatgcactg cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt
    15661 aatggcactt actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc
    15721 accggtcgac tatcacgtac gccgtcatga tgagattttc gcagctatca acgcggtcag
    15781 tgaatcggac gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga
    15841 agaaggggtg attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt
    15901 gccggtattg tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga
    15961 tcaccctgcc ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga
    16021 tgatttactg gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg
    16081 ggtccatgct gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta
    16141 acgcaactga ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac
    16201 tcatcgcgct gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt
    16261 cccccgaagt caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt
    16321 tcgcgcacga gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc
    16381 tgcgtattaa taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg
    16441 aactgaagct gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgtaata
    16501 tgagccgtag cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc
    16561 agggcatcgt ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg
    16621 attccgcaag actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc
    16681 catttcaata agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc
    16741 agcgacaccg gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac
    16801 gttgccgatg tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttacgacatt
    16861 ctgctgctgg gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc
    16921 ttcccgactc tcgaagagat tgatttcaat ggtaaactgg ttgcgctgtt tggttgtggt
    16981 gaccaggaag attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt
    17041 gaaccgcgcg gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca
    17101 tcaaaaggtc tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag
    17161 ccggaactga ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat
    17221 ctcgacgaaa ttcttaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta
    17281 atagataatg ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctata
    17341 caatgtccct gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat
    17401 taacaatatt tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac
    17461 gcattatctc aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag
    17521 gacagattcc gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg
    17581 cttcctcgtt taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg
    17641 gaagatttat acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat
    17701 cgcgtactga accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt
    17761 aaatccgtat ttgaactgac acagcaacat caccacgatc acctgatttg cctcgactgc
    17821 ggcaaggtta tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca
    17881 aaacatggca ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc
    17941 gattgccgcg aagatgagca cgcgcacgaa ggcaaataag ccagcctgaa cgagaaaagc
    18001 caacctgcgg gttggctttt ttatacaagg gaaataagaa aagcgagagt tacagctctc
    18061 acttatttgt tattactgcg tatttcctgc cagaccttat cacaatcggc tttcaccgcc
    18121 tgatcatttc cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcgtacactt
    18181 tcctggtcgg caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg
    18241 caggcttcaa ttttttctgg cgaaccttgc gcggtgttga tacaggcgct gtatgcctct
    18301 ttcagacggc tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc
    18361 atcatggcga tgagaatcag ttttttcatt gaatgcggca ggcacaaaac ctgccgcgtc
    18421 gggcatcaga agatggtgaa tggtgcgatt accataaatt tcacgtcacg ctcatcctgg
    18481 aagatgttac cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg
    18541 aagtgcagtt tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc
    18601 aggctgtagg cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc
    18661 tgccaggtcg caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct
    18721 ggaagattcc agtttttcag gtcatacatc gcactgaaga agaccgcttt ttcgccgtta
    18781 gcgttgaagt cggaacggtt atcccaccag atatccaggc ggccgttgga ggaagcgtag
    18841 gttggggtca tacgttgcag gaagtatccc tgctgactgt cagccttaat ccaggtgcct
    18901 tcgaggcgca aatccactac gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag
    18961 gcggtgccgt cataaagatc gttgacgctg cgatcgtcaa ctttatcgcg ggtaccatag
    19021 aactggtagc tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca
    19081 aaatattgat cgatataccc ttccgcctga ccaaatgccg cttccagtac gaagttattt
    19141 ttgaagtcgt atttcgcacc gatggagtgc agataatcaa ctttggtggt tttatcgttc
    19201 tgataaaact catccatttc cagatgccac ggcgctttgt attcgttggt ccacatgtag
    19261 gagaaactca aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgccccctga
    19321 taagtacccg gcataaagct ccagtgcggc gctaacagcg tttgaccggt tggctgaatg
    19381 taacctgccc tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt atacaggctt
    19441 ataccgcttt tgtcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg
    19501 atttcgttcg ggtgggagct gtcgccgttt tccgccattt caatcgccgt aaacgcagca
    19561 atatcaaggc cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag
    19621 gtggagtgag aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt
    19681 tcacgctgcc agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg
    19741 cgagcctccg gcattgccat aaagcccgac attgctgtaa caccggcaat agccagcgcc
    19801 agcgtactac gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg
    19861 ctgcaccaaa ggtgaacagc gaaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt
    19921 ctcgctgact acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa
    19981 ccagaatgta agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa
    20041 gtttgcggtt gaaaggcttg cagaagaata aaaacgtgaa attccagcaa gatgaaagcg
    20101 gttacatttt atttaattat gttttgtgat tttaaattta ttttatggat aaattgcgaa
    20161 gcggaattaa ttcgcgaaga aaagcagaaa aaacgccgca gagcgcggcg tttgggattg
    20221 tttgctattt cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcacgcaaa
    20281 ttcaatatat tgcagagatc atgtaggcct gataagcgaa gcgcatcagg caatttagcg
    20341 tttgaaattt attgtctatt tattgccgga tgcgacgtaa atgcctcacc cggcttggta
    20401 gaacttaaaa ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa
    20461 ataccggttt ttcggccgtg gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa
    20521 actggaatgc tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga
    20581 tcaccagcga ttccgggtta atcaccgaca ggaaatcatc cgcagcacct gggttaggca
    20641 cgctgaacag acgatcatac aaacggattt caaccggcag cgcatgtgcc gcgctcaccc
    20701 agtgaataac acctttgact ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt
    20761 cataagtaca gaagatggtg gtgatattac cttcggcatc tttctccacg cgttcagcct
    20821 taatgacgta agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct
    20881 tgttagcttc ttcgcggaaa tcggcgcgat cgatccaaat ttcaccgcta aacggcacct
    20941 gacggctccc catttccggt ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc
    21001 cctgatagtt ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggcgcat
    21061 tttcgttgag atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct
    21121 gcttggtcac gccgatgcgt ttgcagaact cacgaataga agccgcagtg taaccacgac
    21181 gacgcagacc ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg
    21241 tcaccagcag gttcagctta cgcttggaca tcacagtgta ttccagattc aggcgcgaga
    21301 attcgtactg gcgcggatga acaggaatgg tgatgttatc cagtacccag tcgtacagac
    21361 gacggttgtc ctggaactca agcgtacaca gagagtgcgt aataccttcc agcgcatcgc
    21421 tgatgcaatg ggtgaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt
    21481 gttcagcaaa tttaatgcgg tacagcaccg gatcgcgcat tacgataaat ggcgacgcca
    21541 tgtcgatttt cgcacgcagg caggctttac cttcttcaaa accaccggca cgcatttttt
    21601 cgaacagcgc caagttctct tcaacgctgc ggtcgcggta cgggctgttt ttacccggct
    21661 gcgtcagggt accgcggtat tcgcggatct gttccggtgt cagttcatca acgtacgcca
    21721 ggcctttatt gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt
    21781 aacggacgtt accagaccag tgaaaaccta accactctac gtcgtttttg atcgaatcga
    21841 catactcgat atcttctttt accgggttag tgtcgtcgaa acgcaggttg cactggcctt
    21901 tatagtcctg ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat
    21961 agccattcgg ctccggcggg aaacgggtgt gtactgtgat gtgcttacca ctggccagat
    22021 cttcatcgat gatctgacgg ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg
    22081 attcctcaaa gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac
    22141 aactgttagt tttttaaaat tgataatcta acaaggatta tggggatgat tgatgaaaaa
    22201 aaaagcggca gagaagatct ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc
    22261 atcaggcata aagcagatta ctttttgatt tcatacagcg gtgtttgacc cgccacaaca
    22321 tggccctgag ctttaatgag caagccactg aaatcgtcga tattgctgca aaccaccggg
    22381 ctaatcatcg agcgggcgtt agcgttcagg taatccagat ccatttccag aatcggttgc
    22441 cctgcgctta cctgcgcacc ctcttccacc agacgtttaa agcctttacc ttccagcgct
    22501 acggtgtcga tacccatatg gacgacgatc tccgcgcctt tttcggtttc caggcagaac
    22561 gcgtggttgg tgttgaagat tttcacgatt gtccctgcgg ctggtgatac gacgatttta
    22621 tctgtcggtt tcaccgccac accgtcaccc accgctttgc tggcgaatgc ttcgtcagga
    22681 acctgatcca gtgccacgac atcaccggta atcggcgata ccagctccgc gatagatacc
    22741 gcgtttggta cagcctgcgg ttttgctaca ggcgcggcag ttgccggagt tgcttcagct
    22801 gacgcagcgg ctaccggacc acgggcaacg actttcttca tcgcatcgcc gatggattct
    22861 gctttcgcgc caacaatcac ctgaatagtc tgtttgttca gtttcactac accagaagca
    22921 cccagacgtt tacacatcgt atcgttgacg cgggcagagt cagccacggt caggcgcaga
    22981 cgggtaatac aggcatcaat cgctttcagg ttgtcagtac cgccaaccgc agcaatatag
    23041 ttggttgcca gttgagtcag accttcttca gtattgctgt tggcttcttc agtaacgatc
    23101 tcgtcttctt tatcttcacg acccggcgtt ttcaggttga acatgcggat aaccaaactg
    23161 aacaccacga agtagatagc gaagaagata acgcccatca ccagcagcat ccagacgttc
    23221 tggctggcgg caggcaggtt atacatcaac gcgtagtcga tagcacccgc agagaaagag
    23281 aagccggcat ggatacccag cagcgttgcc acaaacaggc tgataccggt cagcagtgcg
    23341 tgcaggaggt acagcagcgg cgcaaggaac atgaacagga attccagcgg ctcagtcaca
    23401 ccggtcagga acgcagtaac agcaacagaa agcagcatcc cgccaaccat cggacgacgc
    23461 tctttcggtg ctgcgaagta catcgccagc gccgcacccg gcagaccgaa catcatgatc
    23521 gggaagaagc cggacatgaa catccccgcg gtgccgtcac cagcgtagaa acggttgatg
    23581 tcgccgtgga aaaccgtacc cgccgcgttg gtgaattcac caatctggaa ccaggcgatg
    23641 gtgttcagca cctgatgcag accggttggg atcagcagac ggttgatgaa accaaagata
    23701 ccggaaccca gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat agcgtgctgt
    23761 accggcggcc agacgtaacc aaaaatggcc gccagcacca gacagaagaa gccggtggcg
    23821 atcggcacaa agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt aatatcggac
    23881 caacggttat aggctgcgcc accaaccaga ccggtaatga tacccgccag tacacccatg
    23941 ttaatttctg ggttgatggt caccatcgct ttggttaaca caaagtaacc taccgcaccc
    24001 gccagcgccg ccgcacctgc gctgtctttc gaccagctgg atgccacacc gatggcgaag
    24061 attaatgcga ggttatcaaa aattgcaccg cccgcctggg caataaacgc aacgttaagt
    24121 aaatctggct gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc gatagggagc
    24181 tgtaacgccc taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt ccccctacga
    24241 gaaccctatt tggctcgttt aaagccgtat ttttattttg ctgcaaattt tactgccgat
    24301 gttctgtaat cagattgtta gatcatctgc tacagagtgt gtgaaaattt aattcgcatc
    24361 gcaaattaaa cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc atcactccct
    24421 tttactggct aaaccagaaa acttatttta tcattcaaaa aatcaagtcg gattgacgcc
    24481 tgtctgcgca aatccaggtt acgcttaaag atgtctaatc cgccaacggc ttacatttta
    24541 cttattgagg tgaataatga gactgatccc cctgactacc gctgaacagg tcggcaaatg
    24601 ggctgctcgc catatcgtca atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt
    24661 tgtactgggc ctgccgactg gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat
    24721 gcataaagca ggccaggtca gctttaagca cgttgtcacc ttcaacatgg acgaatatgt
    24781 cggtctgccg aaagagcatc cggaaagcta ctacagcttt atgcaccgta atttcttcga
    24841 tcacgttgat attccagcag aaaacatcaa ccttctcaac ggcaatgccc cggatatcga
    24901 tgccgagtgc cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc atctgtttat
    24961 gggcggtgta ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt ctctggcttc
    25021 tcgtactcgt atcaaaaccc tgactcatga cactcgcgtc gcgaactctc gtttctttga
    25081 taacgatgtc aatcaggtgc caaaatatgc cctgactgtc ggtgttggta cactgctgga
    25141 tgccgaagaa gtgatgatta tggtgctggg tagccagaaa gcactggcgc tgcaggccgc
    25201 cgttgaaggt tgcgtgaacc atatgtggac catcagctgt ctgcaactgc atccgaaagc
    25261 gatcatggtg tgcgatgaac cttccaccat ggagctgaaa gttaagactt taagatattt
    25321 caatgaatta gaagcagaaa atatcaaagg tctgtaattg ttatccctgc cctggttcct
    25381 tgctcagggc aatatttttt aaaatcgggg ggcagaatgt atgcattaac ccagggccgg
    25441 atctttaccg gccacgaatt tcttgatgac cacgcggttg ttatcgctga tggcctgatt
    25501 aaaagcgtct gtccggtagc ggaactgccg ccagagatcg aacaacgttc actgaacggg
    25561 gccattctct cccccggttt tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt
    25621 aacgacaccg ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa agccaatgag
    25681 aaatcaggct gtactaacta tctgccgacg cttatcacca ccagcgatga gctgatgaaa
    25741 cagggcgtgc gcgttatgcg tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg
    25801 catctggaag gtccgtggct gaatctggta aaaaaaggca cccataatcc gaattttgtg
    25861 cgtaagcctg atgccgcgct ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa
    25921 gtgaccctgg caccggaaat ggttcctgcg gaagtcatca gcaaactggc aaatgccggg
    25981 attgtggttt ctgccggtca ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc
    26041 gcggggatta cctttgccac ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa
    26101 ccgggcctgg cgggcgcgat cctcgacgaa gctgacattt attgcggtat tatcgctgat
    26161 ggcctgcatg ttgattacgc caacattcgt aacgctaaac gccttaaagg cgacaaactg
    26221 tgtctggtta ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt catttttgcg
    26281 ggtaaaacaa tatactaccg taacggactt tgtgtggatg agaacggtac gttaagcggt
    26341 tcatccttaa ccatgattga aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg
    26401 gatgaagtgc tgcgtatggc gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt
    26461 ctcggcacgc tcgccgcagg taaagtagcc aacctgaccg cattcacacc tgattttaaa
    26521 atcaccaaga ccatcgttaa cggtaacgag gtcgtaactc aataagagaa agtatgacac
    26581 caggcggaca agctcagata ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg
    26641 tttatcgcct gattgaccag tacgggccaa tctcgcggat tcagattgcc gagcaaagcc
    26701 agcttgcccc cgccagcgta accaaaatta cgcgtcagct tatcgaacgc gggctgatca
    26761 aagaagttga tcagcaggcc tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa
    26821 cccgcaattt ccacgcaatc ggcgtacggc ttggtcgtca tgacgccacc atcactctgt
    26881 ttgatctcag cagcaaagtg ctggcagaag aacattaccc gctgccggaa cgtacccagc
    26941 agacgctgga acatgccctg ctgaatgcca ttgctcagtt tattgatagc taccagcgca
    27001 agctgcgcga gctaatcgcc atttcggtga tcctgccagg gcttgttgac ccggacagcg
    27061 gcaaaattca ttacatgccg catattcagg tagaaaactg ggggctggta gaagcactgg
    27121 aagaacgttt taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg
    27181 agcactactt cggtgcaagt caggattgcg aagactccat tctggtgcgt gtccatcgcg
    27241 gaaccggggc cgggattatc tctaacgggc gcatttttat tggccgcaac ggtaacgtcg
    27301 gtgaaattgg ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg
    27361 gctgcctgga aactatcgcc gccaacgctg ccattgaaca acgggtgttg aatctgttaa
    27421 agcagggcta ccagagccgt gtgccactgg acgactgcac catcaaaact atctgcaaag
    27481 ccgcgaacaa aggcgatagc ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg
    27541 gtaaaaccat cgccattgct atcaacttat ttaatccgca aaaaattgtt attgctggtg
    27601 aaatcaccga agccgataaa gtgctgctcc ctgctattga aagctgcatt aatacccaag
    27661 cgctgaaggc gtttcgtact aatctgccgg tggtacgttc tgagctggat caccgctcgg
    27721 caatcggcgc ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt
    27781 tgctggaaaa ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc
    27841 catgaccatt aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc
    27901 cgtaccgggt gcagcggaat ttttgcacgg gattatggat aaaggcctgc cgctggtgtt
    27961 gctgaccaac tatccttcgc agactgggca agatctggcg aaccgctttg ccactgccgg
    28021 tgtcgatgtg ccggatagcg tgttttatac ctctgcgatg gcgaccgccg atttcctgcg
    28081 tcgctaggaa ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc atgaactgta
    28141 caaagccggt ttcactatta ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg
    28201 ttcctacaac tgggacatga ttcataaagc agcctatttc gtcgctaacg gtgcacgctt
    28261 tatcgccacc aacccggaca cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg
    28321 tgcagggatt gagaaaatct ccgggcgcaa accgttctat gttggtaagc ccagcccgtg
    28381 gatcatccgc gcggcattaa acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg
    28441 cgataacctg cgtaccgata ttctggcggg cttccaggcc ggtctggaga cgattctggt
    28501 gctttctggt gtttcatcgc tcgacgatat cgacagtatg ccgttccgcc caagctggat
    28561 ttacccgtca gtcgctgaaa tcgacgttat ctgaatacca cccacgtctg cggacgtggt
    28621 tggctctgat gacaaacgcc aaactgcctg atgcgctacg cttatcaggc ctacgcagct
    28681 cctgcaatat attgaatttt catgcttttg taggccggat aaggcgttca cgccgcatcc
    28741 ggcatgaaca aatcgcactt tatcagcaat ttggcccccg taaaatataa ccattcaata
    28801 aaacccgcta aaaattatcg attcatcaat aaatacatcc tcacaccaga ccctttttca
    28861 ccaatcgaca atcaccatta cgttttttat ttttgcactg ccaaatagct tgcgcacagt
    28921 gctgctttgc ggcatttttt taaacaagca aacacaacaa gcaacaaata ccaggttaac
    28981 ggagaaggtt atgtgttcaa tttttggcgt attcgatatc aaaacagacg cagttgagct
    29041 gcgtaagaaa gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg
    29101 tatttatgcc agcgataacg ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa
    29161 cgcgggggcg caacctctct acaaccaaca aaaaaaccac gtactggcgg taaacggtga
    29221 aatctacaac caccaggcat tgcgcgctga atatggcgat cgttatcagt tccagacagg
    29281 gtctgactgt gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga
    29341 cttgcagggc atgtttgcct ttgccctgta cgacagcgaa aaagatgcct acctgattgg
    29401 tcgcgaccat ctggggatca tcccactgta tatggggtat gacgaacacg gtcagctgta
    29461 tgtggcctca gaaatgaaat cgctggtgcc agtttgccgc acgattaaag agttcccggc
    29521 ggggagctat ttgtggagtc aggacggcga aatccgttct tattatcatc gcgactggtt
    29581 cgactttgat gcagtgaaag ataacgtgac cgacaaaaac gagctgcgtc aggcactgga
    29641 agattccgtt aaaagccatc tgatgtctga tgtgccttac ggtgtgctac tttctggtgg
    29701 tctggattcc tcaattattt ccgctatcac caagaaatac gcagcccgtc gcgtggaaga
    29761 tcaggaacgc tctgaagcct ggtggccgca gttacactcc tttgctgtag gtctgccggg
    29821 ttcaccggat cttaaagcag cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga
    29881 aattcacttc actgtacagg aaggtctgga tgccatccgc gacgtgattt accacatcga
    29941 aacttatgat gtgaccacta ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat
    30001 caaggcgatg ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg
    30061 ttatctttac ttccataaag cgcccaacgt taaagaactg catgaagaga cggtgcgtaa
    30121 actgctggcc ttgcatatgt atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg
    30181 cgtggaagca cgcgttccgt tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa
    30241 cccgcaggat aaaatgtgcg gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt
    30301 tgagtcatac ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt
    30361 cggttacagt tggatcgaca ccctgaaaga agtggcggcg cagcaggttt ctgatcagca
    30421 actggaaact gcccgcttcc gcttcccgta caacacgccg acctcaaaag aagcgtatct
    30481 gtaccgcgag atctttgaag aactgttccc acttccgagt gccgctgagt gcgtgccggg
    30541 cggtccttcc gtcgcgtgtt cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa
    30601 aatggacgat ccgtctggtc gtgcggttgg cgttcaccag tcggcgtata agtaagaaat
    30661 gcggtactgc cccggagaaa tccggggctg ttttagttat tgcgctgctt gttgattctg
    30721 atatgcagta aggaaatgtt ccgggtttgc cacatcactg tttgacaact catcactgta
    30781 ggccagtatt tcatagatta atttcccacc actattgttg aattccgaat ttcatcctgt
    30841 acgacaaaag aatggctgta gggtggggaa aaacacaaag taaacaataa ttgacgaata
    30901 tagcgccacg ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa
    30961 agaggttgac gctgcaaggc tctatacgca taatgcgccc cgcaacgccg ataaggtatc
    31021 gcgaaaaaaa gatggctacg tagctcagtt ggttagagca catcactcat aatgatgggg
    31081 tcacaggttc gaatcccgtc gtagccacca tcttttttgc gggagtggcg aaattggtag
    31141 acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca
    31201 ccattcacca gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt
    31261 gataccggca ttccctggtt cgaatccagg taccccagcc atcttcttcg agtaagcggt
    31321 tcaccgcccg gttattgggg tatcgccaag cggtaaggca ccggtttttg ataccggcat
    31381 tccctggttc gaatccaggt accccagcca tcgaagaaat aatctggcta cgtagctcag
    31441 ttggttagag cacatcactc ataatgatgg ggtcacaggt tcgaatcccg tcgtagccac
    31501 caaattctga atgtgtcgaa tacgttcggc aaattcaaaa accaatttgt tggggtatcg
    31561 ccaagcggta aggcaccgga ttctgattcc ggcattccga ggttcgaatc ctcgtacccc
    31621 agccaattta ttcaagacgc ttacatcgta agtgcaccca gttggggtat cgccaagcgg
    31681 taaggcaccg gattctgatt ccggcattcc gaggttcgaa tcctcgtacc ccagccacat
    31741 taaaaaagct cgcttcggcg agctttttgc ttttctgcgt tcattcaatg tcgaatgcga
    31801 tgttgacacg tcttatcctt caatgtcgga tgcgacgctg ccgcgtctta tccgacctac
    31861 ggctggcacg catccggcaa tgttgtaagg ctacaaccct aacgcatatt tcagcgcctg
    31921 acgtttcaac acgccagcac gctctgccgc cattaacccg agattacgca caaaacgcag
    31981 cggcggcaga ttattactga atccggcata aaacagatcc ataccacttt gcataatgaa
    32041 gttatccgcc atgcgccgca tctggtaacg cttgaggaca ggataactgg cccacgcttc
    32101 accgtagctg cgggcattca ccagaacatc aatcagggca tcgacatcac gataaccaag
    32161 attcaccccc tgccccgcca gcggatggat ggtatgcgcg gcatcgccca ccagcgcaag
    32221 ccccggctgc acatactgca acgcatgtcg gcgcgtcagc ggaaacgcac cagcggcaag
    32281 cggtgtaacg taacccagac gcgacgggaa atgcttcgcg atttccgcct ggagttgcgc
    32341 catattcata ttctgcaact ggcgaatacg cgtcggagag tcataccaca ccagcgatgc
    32401 ccagttatca aacaacggca gaaacgcacg cggtccgtcc ggggtaaatt gctgccaggt
    32461 gctgtcgccg ggatcgttct cacactgtac gctaatcaac atgcacgact gcgcatactg
    32521 ccaggcatga acgccaattc ccgccatctg ccgcacctga gaatttgcgc cgtcggcacc
    32581 aatcaccagt ttcgcgcgga tcgtttcacc gcccttcagt tccagctcct gaagatcatt
    32641 atctcgatgt aatgcaatca gcgaggttgg cacacgtaac gttactttcg gatgcgcttc
    32701 cagcgcctgc cacaacgcct gttgcaggac tgtgttttcc accatgtagc caagcaacgg
    32761 caactttaat tcggctgcgt caaataccac atgtgccgtt tcccactccc aggtttccag
    32821 tctgcggtaa ggatggcaac gcatagcctg tactgcatcc cagaccccta accctttaag
    32881 caatgatacc gaagccgcgc tgatcgccga gatccgcacg tccggttggc tgtcagcgac
    32941 aaacggtgca ggttctgcgt gctcgatcac cgttaccgaa aatccgtgct gtgccagccc
    33001 cagcgccagt gcgccgccga ccattcctcc gccgacaatg gcaatttccg ttggttgatt
    33061 tgtcatggtc aatcatcctg ttagcaatat gctttaagtt tacaggattt ttacccccaa
    33121 gggctgataa cgctcatact ggtcacaacg gcagcaaagc attacactat gcgccctgca
    33181 ttcctggcta ctatttcgca agagcaagtc gatgaccaaa aaactccata ttaaaacctg
    33241 gggctgtcag atgaacgagt acgattcatc gaagatggcc gatctgctgg atgcctccca
    33301 cggctatcaa ctgaccgacg tggcggaaga agcggatgtg ctactgctga acacctgctc
    33361 aatccgcgag aaggctcagg aaaaagtctt ccatcagttg ggtcgctgga aactgttaaa
    33421 agagaagaat ccagacctga ttatcggcgt cggtggctgc gtggcatcgc aagaaggcga
    33481 gcacattcgc cagcgcgccc actatgtcga tattattttt gggccgcaaa cgctgcaccg
    33541 tctgccggag atgatcaact ccgtgcgcgg cgaccgcagc ccggttgtag atatcagctt
    33601 cccggaaatc gagaagtttg accgtctgcc ggaaccgcgc gccgaagggc cgaccgcgtt
    33661 tgtctccatc atggaaggct gcaataaata ttgcacctac tgcgtggtgc cttacacccg
    33721 tggtgaagag gtaagccgtc cgtctgacga tattctgttt gagattgccc agcttgcggc
    33781 tcagggcgtg cgtgaagtca acctgctcgg tcagaatgtg aatgcctggc gtggtgagaa
    33841 ctacgacggc accaccggat cgtttgccga tctgttgcgt ctggttgctg cgatcgacgg
    33901 gatcgatcgt attcgcttta ccaccagcca tccgatcgaa ttcaccgacg atatcatcga
    33961 agtgtaccgc gacacgcctg agctggtgag cttcctgcat ctgccggtac agagcggttc
    34021 cgatcgcatt ctgaacctga tgggacgtac ccatacggcg ctggagtaca aagcgatcat
    34081 ccgtaaactg cgtgcggcgc gtccggatat tcagatcagt tctgacttca ttgttggctt
    34141 ccctggcgaa accaccgaag acttcgagaa aacgatgaag ctgattgccg acgtcaattt
    34201 cgacatgagc tacagcttta tcttctctgc acgtccagga acgcctgccg ccgatatggt
    34261 tgatgatgtt ccggaagaag agaagaagca gcgtctgtat attctgcaag aacgtatcaa
    34321 tcagcaagcg atggcatgga gccgtcggat gctcggcacc acccagcgta ttctggtaga
    34381 agggacttcg cgtaagagca tcatggagct ttccgggcgt accgaaaata accgcgtggt
    34441 caacttcgag ggcacaccgg atatgatcgg taaattcgtc gatgtagaaa ttaccgacgt
    34501 ctacccgaac tctctgcgcg gtaaagtggt gcgaactgaa gatgaaatgg gtctgcgcat
    34561 ggcagaaaca ccggagtcgg tgattgcccg cacccgcaaa gaaaacgacc ttggcgtggg
    34621 ttattatcag ccgtaattct caggcctgcc gttccggtgg gccttgtttt ttctctcttt
    34681 atccccatct ttatcgcaat gcttgcgcct ttttcctgtg gcgtgaataa tttccttata
    34741 ggccggtgaa tgttcagctt ccggcggcat acagataaga ggaacggttt gaacatagac
    34801 actcgcgaaa tcaccctgga gccagcagac aatgcgcgtc tgttgagcct gtgcggcccg
    34861 tttgatgaca acatcaagca gctcgaacgc cgtctcggca tcgagatcaa tcgccgcgat
    34921 aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg ctgcggcaga cattctgcgt
    34981 agcctgtatg tcgatactgc cccgatgcgc ggtcagattc aggatatcga accggaacag
    35041 atccaccttg cgattaaaga agcgcgggta ctggagcaaa gcgcggagag cgtgccggag
    35101 tacggcaaag cggtcaatat caaaactaaa cgcggcgtaa ttaagccgcg cacgccaaac
    35161 caggcgcagt acatcgccaa tattctcgac catgacatta ccttcggcgt tggcccggcg
    35221 ggtacgggta aaacctacct ggcagtggct gcggcagttg atgccctgga gcgtcaggaa
    35281 attcgccgta ttctgctgac tcgtccggcg gtcgaagccg gtgagaaact gggcttcctg
    35341 cctggcgatt taagccagaa agtagacccg tatctgcgcc cactgtacga cgcgctgttt
    35401 gaaatgctgg gctttgagaa agtcgagaaa ctgattgagc gcaacgttat tgaagtcgcg
    35461 ccgctggcct atatgcgtgg tcgtacgctg aacgacgcgt ttatcattct cgatgagagc
    35521 cagaacacta ccatcgaaca gatgaagatg ttcctgaccc gtatcggttt taactcaaaa
    35581 gcggttatca ccggcgacgt cacacagatc gacctgccgc gtaatactaa atcaggctta
    35641 cgtcatgcca ttgaagtgct ggccgatgtc gaagagatca gctttaactt cttccacagc
    35701 gaagacgtgg ttcgtcaccc ggtggtggcg cgtatcgtta acgcttatga agcctgggaa
    35761 gaagcagaac aaaaacgtaa agcggcgctg gcagcagaac gcaagcgcga agaacaggaa
    35821 caaaaatgag tcaggtgatc ctcgatttac aactggcatg tgaagataat tccgggttac
    35881 cggaagagag ccagtttcaa acatggctga atgcggtgat cccgcagttt caggaagagt
    35941 cggaagtgac tattcgcgtg gtcgataccg ccgaaagcca cagtctgaat ctgacctatc
    36001 gcggtaagga taagccgacc aacgtgctct ccttcccgtt tgaagtgccg cctggcatgg
    36061 agatgtcgct actgggcgat ctggttatct gccgtcaggt ggttgagaag gaagctcagg
    36121 agcaaggcaa accactggag gcgcactggg cgcatatggt ggtgcacggc agtctgcatt
    36181 tgttaggtta cgatcacatt gaagatgacg aagcagaaga aatggaagcc ctcgaaacag
    36241 agattatgct tgctctgggc tatgaggatc cgtacattgc cgagaaagaa taatttgcca
    36301 ggcatttgac tggcagacgt ccccgccgtt ggtgattaac gacggcgttt tgattaacta
    36361 acatgagaac ccatacgacg ccatgagcga cgacaattca cacagtagtg acacgataag
    36421 caacaagaag ggatttttct ccctgttact cagccaactt ttccacggtg aaccgaaaaa
    36481 ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag aacgacctta tcgacgaaga
    36541 tacgcgcgat atgctcgaag gggtgatgga catcgcagac caacgcgttc gcgacatcat
    36601 gatcccccgc tcccagatga ttaccctgaa acgcaaccag acgctggacg aatgccttga
    36661 tgtcatcatc gagtccgccc actcacgttt cccggtgatt agcgaagaca aagatcacat
    36721 tgaagggatt ctgatggcga aagacttgct gccgtttatg cgcagcgatg ctgaagcctt
    36781 cagcatggac aaagtgttac gtcaggcggt tgtcgttcct gaaagtaagc gcgtagaccg
    36841 gatgctgaaa gagtttcgct ctcagcgtta ccacatggcg atcgttattg acgaattcgg
    36901 tggggtttcc ggtctggtga ccattgaaga catcctggaa ctgattgttg gtgagattga
    36961 agacgagtat gacgaagaag atgatatcga cttccgtcag ctgagtcgtc atacctggac
    37021 cgtgcgcgca ctggcttcca ttgaagactt caacgaagcg ttcggcaccc actttagcga
    37081 tgaagaagtc gacactatcg gtggtctggt gatgcaggcg tttgggcatc ttccggcgcg
    37141 tggcgaaact atcgacatcg acggttacca gttcaaagtg gcgatggccg atagtcggcg
    37201 tattattcag gttcatgtca aaatcccgga tgactcaccc cagccaaagc tggatgaata
    37261 aaaccgaaac tggatagata actacatgga ttttgcctca ttaattgaac gccagcgcat
    37321 tcgcctgctg ctggcgttat tattcggtgc ctgcggaacg ctggccttct ctccttacga
    37381 cgtctggcct gcggcgatta tttcgctgat ggggcttcag gcgttgacct ttaaccgtcg
    37441 cccactccag tctgccgcta ttggcttttg ctggggattt ggcctctttg gcagcggtat
    37501 taactgggtc tatgtcagca tcgcgacctt tggcgggatg cctggcccgg ttaacatctt
    37561 cctggtggtg ctgctggcgg cgtatttgtc gctgtatacc ggactgtttg ctggcgtgct
    37621 gtcgcgtctg tggccgaaaa ccacctggct gcgcgtggcg attgccaccc ctgctctctg
    37681 gcaagtgacc gagtttctgc gcggttgggt gctgactggc ttcccgtggt tacagttcgg
    37741 ctatagccag attgatggcc cgttaaaagg gctggcaccg ctaatgggcg tggaagccat
    37801 taacttcctg ctgatgatgg ttagtggcct gctggcactg gcgttggtca aacgcaactg
    37861 gcgtccgctg gtggtggccg tcgtgctgtt tgccctaccc ttcccgctgc gttacatcca
    37921 atggtttacc ccgcaaccgg agaaaaccat tcaggtttcg atggttcagg gcgatattcc
    37981 gcaatcgctg aaatgggacg aaggccagct tcttaatacg ctaaagattt actacaacgc
    38041 aacggcaccg ctgatgggca aatcatcgtt gattatctgg ccggagtcgg cgataaccga
    38101 tctggaaatt aatcagcaac cgttcctcaa agcactggac ggtgagttgc gtgataaagg
    38161 tagctcgctg gtaaccggga ttgtcgacgc gcgtctcaat aagcagaacc gctacgatac
    38221 ctacaacacc atcatcacgc tgggtaaagg tgcaccgtac agctacgaat cagccgatcg
    38281 ctataacaaa aaccatctgg tgccgtttgg cgagtttgtc ccgctggagt cgattctgcg
    38341 tccgttagca ccgttctttg atctgccgat gtcgtcgttc agccgtgggc catatatcca
    38401 gccgccgctg tcggcaaatg gtattgagct tactgcggct atttgctacg agattattct
    38461 cgccgagcaa gtgcgcgata acttccgccc ggataccgac tacctgctga ctatctccaa
    38521 cgatgcgtgg tttggtaaat ctattggtcc atggcaacac ttccagatgg cgcgaatgcg
    38581 tgcgctggag ctggcgcgcc cactgttgcg cagcaccaac aacggcatta cggcggtgat
    38641 tggcccgcag ggtgagattc aggcgatgat cccgcagttc gcccgcgagg tgttaaccac
    38701 taacgtgacg ccgaccaccg gactcacacc atacgcacgt accggcaact ggccgctgtg
    38761 ggtgctgacg gcactgtttg gttttgctgc tgtgttgatg agtctgcgtc agcgacgtaa
    38821 ataatccctt cttatcgtgc cggaatacat cccatatttc ggcacgctca ccggatgcgg
    38881 catcagtgca ttatccggat aacaatattc tcctatccaa ctttggcaca tctattgctt
    38941 tgttatacaa ggcaaagctt gaaccagcat cagtacaacc cggttgcacc acagcaatcc
    39001 accggtagca ccaaaagagt gcattttaag aattttgctt cagaatggtg cggtgcacct
    39061 cgcaaaaaca aacaataaaa catcaatatc tttacattta gccaaactaa atgttaccta
    39121 acaggcacaa cactgcacaa taaagttgca gacgataaca acacaaacac tcacaacggg
    39181 tatccatgcg ttcttaacgc agaagataaa ggtgttggat atgcaattac gtaaacctgc
    39241 cacagcaatc ctcgccctgg cgctttccgc aggactggca caggcagatg acgccgcccc
    39301 ggcagcaggc agtacgctgg acaaaatcgc caaaaacggt gtgattgtcg tcggtcaccg
    39361 tgaatcttca gtacctttct cttattacga caatcagcaa aaagtggtgg gttactcgca
    39421 ggattactcc aacgccattg ttgaagcagt gaaaaagaaa ctcaacaaac cggacttgca
    39481 ggtaaaactg attccgatta cctcacaaaa tcgtattcca ctgctgcaaa acggcacttt
    39541 cgattttgaa tgtggttcta ccaccaacaa cgtcgaacgc caaaaacagg cggctttctc
    39601 tgacactatt ttcgtggtcg gtacgcgcct gttgaccaaa aagggcggcg atatcaaaga
    39661 ttttgccgac ctgaaaggca aagccgtagt cgtcacttcc ggcactacct ctgaagtttt
    39721 gctcaacaaa ctgaatgaag agcaaaaaat gaatatgcgc atcatcagcg ccaaagatca
    39781 cggtgactct ttccgcaccc tggaaagcgg tcgtgccgtt gcctttatga tggatgacgc
    39841 tctgctggcc ggtgaacgtg cgaaagcgaa gaaaccagac aactgggata tcgtcggcaa
    39901 gccgcagtct caggaagcct acggttgcat gttgcgtaaa gacgatccgc agttcaaaaa
    39961 gctgatggat gacactatcg ctcaggtgca gacctccggt gaagcggaaa aatggtttga
    40021 taaatggttc aaaaatccaa ttccgccgaa aaacctgaac atgaatttcg aactgtcaga
    40081 cgaaatgaaa gcactgttca aagaaccgaa tgacaaggca ctgaactaat tacaagaacc
    40141 aggggcggaa attccagccc tctcgattgt tacgtagcac ggacagacta tacgcctgat
    40201 ggtcgttccc catcgggcct gaaaaccgca atacgctggg taacaatctt cgagggtagc
    40261 agttaacgct gctaccctct tttttctgga gtagatttat gtctatagac tggaactggg
    40321 gtattttttt acaacaagcc ccgttcggca ataccaccta tcttggttgg gtctggagtg
    40381 gttttcaggt gacgatcgct ttgtcgatct gcgcctggat tatcgctttc ctcgtcggtt
    40441 catttttcgg cattttacgt accgttccaa accgttttct ctccggtctg ggtacgttgt
    40501 atgtcgaact gttccgcaac gtgccgttga ttgtgcaatt ctttacctgg tatctggtga
    40561 tcccggagct gctgccggag aaaatcggca tgtggtttaa ggccgagctg gatcccaata
    40621 ttcagttttt cctttcatcc atgctctgcc tggggctgtt taccgccgcc cgtgtttgcg
    40681 aacaggtacg cgccgctatt cagtcgctac cgcgtgggca gaagaatgcc gcgctggcga
    40741 tgggcctgac gctaccgcag acttatcgat acgtactgct gcctaatgct tatcgcgtta
    40801 tcgtcccgcc gatgacttcc gagatgatga acctggtgaa aaactccgcc atcgcctcga
    40861 ccatcggtct ggtggatatg gcggcgcagg cgggtaaatt gctcgactac tcggctcacg
    40921 cctgggagtc gtttaccgcc atcacgcttg cgtatgtgtt gattaacgct tttatcatgc
    40981 tggtgatgac gctggttgaa cgtaaagttc gcctgcccgg caacatgggg ggcaaataat
    41041 gtacgagttt gactggagtt ccattgttcc ttccctgcca tatctgctcg acgggctggt
    41101 gatcaccctg aaaatcaccg tcacggcagt cgtgattggt attttgtggg gtaccatgct
    41161 ggcggtgatg cgcttgtcca gcttcgcgcc tgtttcgtgg tttgccaaag cctacgttaa
    41221 cgtattccgc tcgattcctt tagtcatggt tttgctgtgg ttttacttga ttgtgccggg
    41281 atttctgcaa aatgtgctgg gattatcgcc aaaaaatgat attcgtttaa tttcggcgat
    41341 ggtggcgttt tcgatgtttg aagcggccta ctattcagag attatccgtg ccggtattca
    41401 aagtatttcc cgtggtcagt cgagcgccgc gctggcgttg gggatgactc actggcagtc
    41461 gatgaaactg attattctgc cgcaggcttt ccgcgcgatg gtgccgctgc tgctcactca
    41521 gggcatcgta ctgttccagg atacctcgct ggtgtatgtg ttatctctgg ccgatttctt
    41581 ccgtaccgcc tcaaccattg gtgagcgtga tggtacgcag gtcgagatga tcctgtttgc
    41641 cggatttgtt tatttcgtta ttagccttag cgcgtcgttg ttggtcagct acttgaaaag
    41701 aaggacagca taatgattac cctgaaaaat gtttcaaaat ggtatggtca ctttcaggtg
    41761 ctgaccgact gctcaaccga agtgaaaaaa ggcgaagtgg tggtggtttg cggcccgtct
    41821 ggttccggca aatcaacgct gattaaaacc gtcaacggcc tcgaaccagt gcagcaaggt
    41881 gaaatcaccg tcgatggtat cgtggttaac gacaagaaaa ccgatctggc aaagctgcgc
    41941 tcccgcgtcg ggatggtatt ccagcatttc gagctgttcc ctcatctgtc gattatcgaa
    42001 aacctgaccc tggcgcaggt gaaagtgctt aaacgcgata aagcgcccgc tcgtgaaaaa
    42061 gcattgaaat tgctggagcg cgtgggactt tccgcacatg ccaataaatt tccggcgcag
    42121 ctttccggcg gtcagcagca gcgcgtggca atcgctcgcg cattgtgtat ggctcctatt
    42181 gcgatgctgt tcgacgaacc gacatcggcg ctggatccgg agatgatcaa cgaagtgctg
    42241 gacgtgatgg tggaactggc gaacgaagga atgaccatga tggtggtgac ccacgaaatg
    42301 ggctttgccc gcaaagtggc gaatcgggtg atctttatgg acgagggtaa aattgtcgaa
    42361 gactcgccga aagacgcttt cttcgatgat ccgaaatcgg accgcgcaaa agacttcctc
    42421 gcgaaaatcc tgcattaatc actctggcgc acatacccaa ttgcgcgcca tctcacgctt
    42481 tatcgtttcg cccctcgtat ctgttacatc ctggcgttac ccttgtcgca aagaagcaca
    42541 caacaaggag caacaatggc actgccaatt ctgttagatt gcgacccagg tcatgatgac
    42601 gctatcgcaa tagttctcgc actcgcctca ccagagcttg atgtcaaagc aattacgtct
    42661 tccgccggaa accagacacc agaaaaaacc ttacgcaatg ttctgcgtat gctgaccttg
    42721 cttaatcgca ccgatattcc ggtggcaggc ggcgcggtaa aaccgttaat gcgtgagttg
    42781 attatcgcgg acaatgtgca cggcgaaagc ggtctcgacg gcccggcatt accggaaccg
    42841 acgttcgcac cgcaaaactg tacggcggtg gagctgatgg cgaaaacgct gcgcgaaagt
    42901 gcggaacctg tcacgattgt ttctaccgga ccgcaaacta acgttgcctt gctgctcaat
    42961 agccatccgg aactgcatag caaaattgcc cgtatcgtga ttatgggcgg cgcaatgggg
    43021 cttggtaact ggacgcctgc ggctgaattt aatatttacg ttgacccgga agcggcagaa
    43081 attgtcttcc agtcagggat cccggtggtg atggccggtc tggatgttac tcataaagcg
    43141 caaatccacg ttgaagacac cgagcgtttc cgcgcgattg gcaaccctgt ttcaaccatt
    43201 gttgccgaac tgctggattt cttcctcgaa tatcataaag acgaaaaatg gggctttgtc
    43261 ggcgcaccgc tgcatgaccc atgcaccatc gcctggctgt tgaaacccga gttgtttacc
    43321 actgttgagc gctgggttgg cgtggaaaca caggggaaat atacccaggg tatgacggtt
    43381 gttgattatt attatctgac tggcaataaa ccgaatgcca ctgtaattgt cgatgttgat
    43441 cgtcagggct ttgttgattt actggctgat cgtctgaaat tttacgctta acactcgtca
    43501 ttgattaaga tcaccacgcg ttacctgggt ggtcttaatc tatttatctc tttttctttc
    43561 aagatataaa tgcaatggat aatgcagaac tcgccatagg tatcgatctc ggcactacca
    43621 atagtttaat tgccgtctgg aaagacggtg ccgcgcaatt aattccaaat aagttcggtg
    43681 aatatttaac accatccata attagcatgg atgaaaataa tcatatttta gtcggaaaac
    43741 cggctgtatc acggcgtact tcgcatccgg ataaaacggc agcgttattt aaacgtgcaa
    43801 tgggcagtaa taccaactgg cggttaggca gcgacacatt taacgcgcca gaactgtcct
    43861 ctttggtatt acgctcatta aaagaagatg ccgaagaatt tctgcaacgt ccgattaaag
    43921 atgtggtgat ctccgttctg gcttatttca gcgatgaaca acgcaagcat acccgtttag
    43981 cagcggactt agccgggtta aatgcggtac gcttaattaa tgaacccaca gcagctgcga
    44041 tggcgtatgg cctgcatacc caacaaaata cccgttcgct ggtgtttgat ctcggtggcg
    44101 gcacgtttga cgttacggtg cttgagtacg ccacgccggt gattgaagtt cacgcctccg
    44161 ctggcgacaa cttcctcggt ggcgaagatt ttacccatat gttggtcgat gacgttttaa
    44221 agcgcgcgga tgtcacaagg accacgctta acgagagtga actggcagcc ttgtacgcct
    44281 gtgtggaagc ggcaaaatgt agcaatcaat cgccaatgca cattcgctgg tagtaccagg
    44341 atgaaacgcg ggaatgcgaa ttttacgaga acgaactgga agatttgtgg ttgccgctgc
    44401 tcaatcgctt gcgagtgccg attgaacagg cgttgcgcga tgcgcgtctg aagccgagtc
    44461 aaatcgacag tctggtgctg gttggcggcg cgtcacaaat gccgctggtg cagcgaatcg
    44521 ccgtgcgtct gtttggcaaa ttaccgtatc aaagttacga tccgagcacc attgtcgcgc
    44581 tgggcgcagc aatccaggcc gcctgccgct tacgcagtga agatattgaa gaagtgatcc
    44641 tcactgatat ttgcccttac tcgttgggcg ttgaggttaa ccgccagggc gtttccggca
    44701 ttttctcgcc gattattgaa cgaaacacca ctgtgcccgt gtcgcgtgta gaaacttatt
    44761 caaccatgca cccggaacag gattcaatta cggttaacgt ctatcaggga gaaaaccaca
    44821 aagttaaaaa caacattctg gtggaatcct tcgatgtgcc gttgaagaaa accggggctt
    44881 atcagtcgat tgatattcgc tttagttatg atatcaacgg gttgcttgaa gttgacgtgc
    44941 ttctggaaga cggcagcgtt aagtccagag tgattaacca cagcccggta acattgagcg
    45001 cgcagcagat tgaagagagt cggacgcggt tatccgcatt gaaaatttat ccgcgcgata
    45061 tgctcatcaa tcgcaccttt aaagccaaac tggaagagtt gtgggcgcgg gcgctgggtg
    45121 acgagcgaga agagattggc cgggtgatca ccgattttga tgcggcgctg cagtcaaacg
    45181 atatggcccg cgtcgatgag gtcaggcggc gggcgagcgt ttatttagcc attgagacct
    45241 cataatcacg gctgccggat gcggcgtaaa tgctgcatcc ggcatcggcg caaaaaacac
    45301 atcgtcaact aaacaacatc gcgattttgc cgagagcata acctataacc agcaccgtta
    45361 aactgatttt gataatgctg gtaatgctct gtggtttttc accctcatca tttttttctt
    45421 catggttttc aggtgttggc tcatcgacat cttcatcctc cggctcgagt tttactttcg
    45481 tcaacagcat ccgggtgata atggcctcta atggatgagc atctccgcct tccagccttg
    45541 tattaagacg ggccagcatc tcttgtttcg gaaacgcttc aagtactacc tgttccgcta
    45601 cccgatacaa atccagtaac ggtgaatctt cttctgtctc gctaccgact ctcgatttcc
    45661 accacgccca tgctttgcgt ttgaagaata acgactcaat aaacgattga cctaacggcg
    45721 ataattttcc gctatgtaaa atttccgccc ataacagccg cgtccgggcg ctctcaccag
    45781 gaacatattt cagcggttga ccttgcgcat ctaccatgct ccgcgcttcg ataaccatca
    45841 ctaataacgc gaaataatcc gggcaatgac gcttgctcca ctgcaacaac aaattatcag
    45901 cttgtgtaga tgggtaactt tcccagtacg cgccgagatc agcaagcagg ctttccccct
    45961 cgccgcagta caaacgttgt gcacactggt aataccgggc atcttcgctt tctggttctt
    46021 ccgcttgcca ctgtttggct acaggaatga gttccaccat acctgtttgc accgagctgt
    46081 accagcgcaa cagtttgcga tgtaactttg catcatcagg aatgaaccac ggtccttcca
    46141 tcgccaaaaa cgcagtgaca tagtcaggat ggtaacggca gcaacgttcc agcgcgaagt
    46201 aatattcaac agtctggttc tgtaacgcga cgggatattc cagaaggcta aggaaatcga
    46261 aacaatcacc agcctgaatg gcctcaagaa aagcctccct ttcttcctca tccattcctt
    46321 cgctgtcatt aaacgaatgc cagttcaaac gctctgccaa caaagaagca caatttaatg
    46381 acagatatcg cgcttctata gcgattgcac acagcggcca gcgtaattga tcgacatcct
    46441 ccatgttcca ggtatttaat tgctgaataa atttctgcca ggcggagggc tgaaaacgat
    46501 cactttctga atccagtaat gtcctgaatg cacgtagtat ttcatgttca gcggtggcat
    46561 ctttttcttc atcatctgct tcctcgacag gatttaccgc cagtcgcagc gcctcttcat
    46621 aggcctggcg taatgcttta aacccctgcg gatcggtttc gggatgacac aacggtaagc
    46681 gagcaagata agcctggcgg ataatgtcta tttgcgtcgt gctttcaatt tcgaggattt
    46741 gccaacatgt tttcataggt cgctcacatc cttaagcgtt accgggcatt caggctggac
    46801 agttaatggc tgccagggaa gcgtggcata gaaacggtcg cttcccgctg ccagctcttc
    46861 gaacatccga ctcccggcgc tatcgtattt gtacagggtt agcgcctcgc gtagcgaagc
    46921 gttgtcttcg cactgagaag attgccagta gagacgccct agtgaatagg cagaaaaata
    46981 gtgcatccag ctgtgatagt aatggtgagc tcgtgcatag acgcgcgatt gcagccataa
    47041 actctcttct tccgtcagcc agttgttgag cacaccggcc cgtgacagaa atcccattcg
    47101 aacataatcc caggctttga ttcccccttc tccacagcac atagcggttt gggcaacgaa
    47161 ttgggtataa atcaaccctc tctccgagcc accagcaatt aatgctttcc actcttcagg
    47221 ggaagagcgt tgccatgtca gatataagcc agctaaatca ttggcatgac catcatcagt
    47281 catctgataa atagtctgaa ttaaatcatc acgattattt attccccagg atgatgagag
    47341 atcgaattct tttatcccgg tatacatttt tggcgagtca tgcgcgggtt tgcaactctg
    47401 attaagtatt tccatgggcg cagaaagcgc aaaaagaaga tattgttctt ccttattcat
    47461 tttttaataa tcccttaagt gcattaactg actacttttt tattattgat gagtataaac
    47521 ttgacgcttt caaaataaaa agaaaatcga agcattcaca catgaataaa aaattaatgt
    47581 atatattcgc aatttttata gttgcagcaa ttacctgtat tagccaaccc aagaaaacga
    47641 tgttgcgtga taaagccatg gtgaattatg cctttgatta tttaagatca ccaggcagtc
    47701 taccgttaac cacggcagcc acggagcttt ccgcgattca tggtcactca acatcgcaat
    47761 atcgccttgg cgaattttat cttcatggta gcgacggtaa accactggat tatacacagg
    47821 cgagatactg gtatgagcaa tcagcagaac aggaaaatcc gcgcgcacaa agcaaactgg
    47881 ggtggatcta cctgaaaggc ctgggagtca aacccgatac ccgtaaagca attctctggt
    47941 ataaggaagc agctgaacaa gggtatgctc atgctcaata tactttgggt ttgatctaca
    48001 gaaatggcac tggtattaat gttaaccatt atgaatctca aaaatggtta aaactggccg
    48061 ccaaacaaca ttacaaaaat gcggaaagat tacttgccgg acttcccgca cattaacgtc
    48121 gactgtcacc cgaagagatg gagtattttc cctatcaaac cggcaaagat aaaaatgtag
    48181 aaaataattt tgatgacgcc cagacagccc agcccgccat catcaacggg cattggctgc
    48241 ggttgcggct ccggctcgac ggtttcctga ccggtaaaat tcgccgcctg aattaataat
    48301 cgacgaataa ttaatgcttc cagtggacaa cgatcttctg catcaaggcg gctcacaatc
    48361 ggcattaatg attcttctgt cggttggctg gcctgcacta attgtttggc aactaaatat
    48421 aatggccata agggatgttc tgtatcacgc gtgagtgaga tccgcggctt acgtcctttg
    48481 tgcaaaacca tctcgaccag tgaacgcccc agcgcagaga gtttttcatc gctgagtatt
    48541 tcgtataaat gaaaacgcgt acgggcgctg tcgccgggga cataaagtag cggttttccc
    48601 tgatcgttaa ccagatcacg cgcttcaatc atcatcacca ctaatgggta ataatagacc
    48661 cgatgctgac ggcaccattg caacattaaa gcatccgcct gggtggaggg atactcgcgc
    48721 cagtagtaac aaagttctgc cagcaggctt tcgccttctc cacaatagac gcgttgtgcg
    48781 tattcataat aacgcgcatc ttcattatca ggttcagctt cccgccacga ccgggcgtaa
    48841 tccaggagtt cggggattcc caggcggctt gcgctatacc agcggagtaa atttctgtgc
    48901 agttttgcat catcggggat aatcactgcg cgatgttgcg ctaaatacaa tgtcagccat
    48961 tctggatgcg atgaccaaat ccgagcgaac atttggtaaa aatcgatggt gtcattttgc
    49021 acggcaactg gcagatgcag aatggtctgg aagttaaaaa cattcccctt cgcaatggca
    49081 tataaaaagg attccagttc ttcttcgtct atttccccga cgttttcctc ctgcaaccac
    49141 cgcaatcttt ctgctaataa caccacgcac tcgaaggata aatgggcagt gttcatggcg
    49201 attgtgcaca gcgaccagcg taattcatca atctcttcca tcgagcaata atttaattgc
    49261 tgaatgaatc gctgccaggc ggagggcaga aaacgttcgc tatcagaggc aagtaacgcg
    49321 cgaaaggcga gcagaatttc atgttctgct acctcatatt cttctggttg ccaaacagat
    49381 ttagcaggcg actgcgcaat ccgtagcgcc tcctcatacg cctgacgaag ttgtttaaaa
    49441 ccctgcggat cggtttctgg atgaaaggac ggtaacagcg ccagataagc gcggcggata
    49501 atatcgacgt cagtcgtttc ctctatatcg aggatcttcc agcaattttt cataagtcgc
    49561 tcatatcctt aagcgtttcc gggcatgtgg ggtagtcagc cagtggttgc caggacaagg
    49621 tagggtaata cgcatcatca ctggcaaata attgataaaa catatcattg ccggaatcgt
    49681 cgtattcttt acgcgctaag aattcgcgta ataaaggcag atgatcatct tcgggagact
    49741 gccaatattg ccgaccaaag gtataaccgg caaagtattg ccgccagtta ctataataac
    49801 gtagagcccg tagatgaata cgcgactgga tccatagact ttcttcttca ctcaaccagt
    49861 tattaagtac gcccattcga cttaaaaaac ccatccggac ataatcccat gctttaattc
    49921 ctccctcacc acagcattcg gctgtgctgg cgacaaattg cgcataagcc tgaccttgct
    49981 catttaattc agcaagatag tcacgccatt cacatggaga ataacgaaac cagcggtgat
    50041 aaaaccctgc caaccgagcg gcatgaccgt tctcgatcat tcggtgaatt atgcgcagca
    50101 ggtcatcacg gtcatcaatt ccccatgagg aagaaaggtc aaaaaagcat gtacctaaat
    50161 acattttggg ggatgaatgc gaaggtaaac agcattcatt gcgtatttcc atcggagtgg
    50221 aaagtgcaaa caatattttt tgcgacccca tgtccatata atttatcctt aaacattcat
    50281 ttaatgaatg taaactacat ttcgaggctc agttatttat acttttctga cgcaacataa
    50341 tacgcataaa ataaggctta cgcaaaaatg gaacacataa aaatataata tattttatct
    50401 acaaccataa ggaaatgata atgattttta cgtcaagttg ctgcgataat ttatcaatag
    50461 atgagattat cgaacgtgct gaaaaaggcg attgcgaggc tcaatatatt gttgggtttt
    50521 attataatcg cgatagcgca attgactctc cggacgacga aaaggccttt tactggctga
    50581 agctggccgc tgagcaaggt cattgtgaag cacagtattc cttagggcaa aagtataccg
    50641 aggataaaag ccgtcataaa gataatgagc aagccatctt ctggctgaaa aaagctgccc
    50701 tacaaggcca tactttcgct tccaacgccc ttggctggat actggatcgt ggagaagacc
    50761 ccaactataa agaagcggtt acctggtatc agatagccgc ggagagcgga atgtcttatg
    50821 cgcaaaataa tcttggatgg atgtacagaa atggcaacgg agtcgcacaa gactatacgc
    50881 tggcattttt ctggtacaaa caagctgcat tacaaggcca tagttacgca caagacaatc
    50941 tggccgatct ttataaagac ggagaaggcg ttgctcaaaa caagacactc gccgcattct
    51001 ggtatttgaa aagcgcacag cagggtaatc ggcacgccca gtttcaaatt gcatgggatt
    51061 ataacgctgg cgaaggggtg gaccaggact ataagcaagc gatgtactgg tatctgaagg
    51121 ctgccgctca ggaaagcgtc ggcgcttacg tcaacatcgg ttatatgtat aaacacggac
    51181 aaggcgttga gaaagattat caggctgcct ttgaatggtt tacgaaagcc gctgaatgca
    51241 atgacgccac tgcctggtat aacctggcca ttatgtatca ctacggagaa ggaagacctg
    51301 tcgatctccg acaggctctc gacctgtatc gtaaagttca gtcatccgga accagagatg
    51361 tcagtcagga aattcgtgag actgaagctt tactgtagaa ataaaaacaa gggccgggga
    51421 atttcattct gcccatattc tctttattgc tgtgcgaaat cagactatta cggctcaaac
    51481 gggcgtctct ggaactggtc ataaccacat ttcgggcata gcgtcagcac ttccggtgtg
    51541 tagatcggga gatggaagtg acatttctcg cagaccagat ttcccagccc gaccacttct
    51601 ccgctgtgat aaaccccatg atgattgagg tcctggaaaa tttcgcgcca ttcaagctgc
    51661 gttttatcgg tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag
    51721 acgctgtcag attcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca
    51781 cgtctgacag ctcgcgtcag ctcatcgatc tcggttcgcg ttaactcccc tgtttttatt
    51841 acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt
    51901 tcgttcagtg acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct
    51961 cccgggtcgt taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc
    52021 ccacaaactc tgtaaattaa tgtacgaata atccgggcat catttcaacg tcgagtgcgc
    52081 gaaaggctgt tttgacgcgg acgtttgggc tatgctatgc ggatctgaaa aaccacctca
    52141 acgctacatt tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc
    52201 gcccggaaga gatagaatcc aaagtacagc ttcattggga tgagaagcgc acatttgaag
    52261 taaccgaaga cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg
    52321 gtcgactaca catgggccac gtacgtaact acaccatcgg tgacgtgatc gcccgttacc
    52381 agcgcatgct gggcaaaaac gttctgcagc cgatcggctg ggacgcgttt ggtctgcctg
    52441 cggaaggcgc ggcggtgaaa aacaacaccg ctccggcacc gtggacgtac gacaacatcg
    52501 cgtatatgaa aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg
    52561 caacctgtac gccggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa
    52621 aaggcctggt atataaaaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac
    52681 tggcgaacga acaagttatc gacggctgct gctggcgctg cgataccaaa gttgaacgta
    52741 aagagatccc gcagtggttt atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc
    52801 tggataaact ggatcactgg ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc
    52861 gttccgaagg cgtggagatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt
    52921 acactacccg cccggacacc tttatgggtt gtacctacct ggcggtagct gcgggtcatc
    52981 cgctggcgca gaaagcggcg gaaaataatc ctgaactggc ggcctttatt gacgaatgcc
    53041 gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg
    53101 gctttaaagc tgttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg
    53161 tattgatgga gtacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact
    53221 acgagtttgc ctctaaatac ggcctgaaca tcaaaccggt tatcctggcg gctgacggct
    53281 ctgagccaga tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg
    53341 agttcaacgg tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga
    53401 tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgccagc
    53461 gttactgggg cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccaaccc
    53521 cggacgacca gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc
    53581 cgattaaagc agatccggag tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg
    53641 aaaccgacac tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc
    53701 cagagtacaa agaaggtatg ctggattccg aagcggctaa ctactggctg ccggtggata
    53761 tctacattgg tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca
    53821 aactgatgcg tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc
    53881 agggtatggt gctggcagat gccttctact atgttggcga aaacggcgaa cgtaactggg
    53941 tttccccggt tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag
    54001 atgcggcagg ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca
    54061 acggtatcga cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta
    54121 tgatgtttgc ttctccggct gatatgactc tcgaatggca ggaatccggt gtggaagggg
    54181 ctaaccgctt cctgaaacgt gtctggaaac tggtgtacga gcacacagca aaaggtgatg
    54241 ttgcggcact gaacgttgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc
    54301 ataaaactat cgctaaagtg accgatgata tcggccgtcg tcagaccttc aacaccgcaa
    54361 ttgcggcgat tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg
    54421 atcgcgctct gatgcaggaa gcactgcagg ccgttgtccg tatgctcaac ccgttcaccc
    54481 cgcacatctg cttcacgctg tggcaggaac tcaaaggcga aggcgatatc gacaacgcgc
    54541 cgtggccggt tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg
    54601 ttaacggtaa agtccgtgcc aaaatcaccg tcccggtgga cgcaacggaa gaacaggttc
    54661 gcgaacgtgc tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta
    54721 aagtgattta cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag
    54781 cgtgcgatat ctggcaacac tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg
    54841 ctggcatctg cgtgacacca cgcaggttcc ttccactatg aaggtcatga tcctggactc
    54901 aggcgatccg aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt
    54961 cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt
    55021 gagcatcgcg aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat
    55081 gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt gatatctacc cgattagcgc
    55141 caaagtcttc cgttcattct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca
    55201 agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc
    55261 aagcatccgt gctgcggata ttcgttccga cgaagaacag acgtcgacca caacggatac
    55321 tccggcaacg cctgcacgcg tctccaccat gctgggtaac tgatgattcg gttgtacccg
    55381 gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac
    55441 gatcctctgt tattgcagga aagccaggac gctgttcgcc aggtagctgc ggcacaagga
    55501 ttcgaagaac accacacttt ttccattgat cccaacactg actggaatgc gatcttttcg
    55561 ttatgccagg ctatgagtct gtttgccagt cgacaaacgc tattgctgtt gttaccagaa
    55621 aacggaccga atgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac
    55681 gacctgctat tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg
    55741 tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag
    55801 cttccccgct gggttgctgt gcgcgcaaaa cagctcaact tagaactgga tgacgcggcg
    55861 aatcaggtgc tctgctactg ttatgaaggt aacctgctgg cgctggctca ggcactggag
    55921 cgtttatcgc tgctctggcc agacggcaaa ctgacattac cgcgcgttga acaggcggtg
    55981 aatgatgccg cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt
    56041 aagcgcgcgt tgcatattct tcagcaactg cgtctggaag gcagcgagcc ggttattttg
    56101 ttgcgcacat tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat
    56161 acgccactgc gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg
    56221 ggcgaggcgt taaatcgctt aagtcagccg cagttacgtc aggccgtgca actcctgaca
    56281 cgaacggaac tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg
    56341 ttatctcttc tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa
    56401 atctttacag gctctgtttg gcggcacctt tgatccggtg cactatggtc atctaaaacc
    56461 cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt
    56521 tcctccgcat cgtccccagc cggaagcgaa cagcgtgcag cgtaaacaca tgcttgaact
    56581 ggcgattgcc gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc
    56641 ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa caaggaccgg acgtgccgct
    56701 ggcgtttatt attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac
    56761 gatactcgac aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc
    56821 gcaaccgcaa taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca
    56881 ccttcagcct gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac
    56941 catcatccgc gaacgtttgc aaaacggtga atcgtgtgag gatttattgc cggaaccggt
    57001 attgacttac attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg
    57061 atgcgactgt ggttaattcg tcatggcgaa acacaagcga atgtcgatgg tctgtacagc
    57121 ggtcatgcgc ccacacccct gaccgcacgc ggtattgagc aagcgcaaaa tctgcataca
    57181 ctgctagatg atgtttcctt tgacttggtt ttatgcagtg aactggaacg ggcacagcat
    57241 accgcgcgac tggttctcag tgaccgccag ctccccgttc atatcatccc tgaactcaac
    57301 gaaatgtttt ttggcgactg ggagatgcga catcatcgcg acctcatgca agaagatgcc
    57361 gaaaactata gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga
    57421 tttcaggcat tttcgcaacg tgtggaacgc tttatcgcaa ggcttagtga atatcagcac
    57481 tatcagaata ttttaatcgt cagccatcag ggcgtactga gtctgttaat tgctcgttta
    57541 attggcatgc ctgccgagtc tatgtggcat tttcgcgttg accaggggtg ttggagcgcc
    57601 attgatatca accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc
    57661 gagaacgcat gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg
    57721 ggcaggctga tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg
    57781 ttttacaaaa atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa
    57841 gccgattcgg cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt
    57901 tttatctatt tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt
    57961 tatcgacaaa attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcaaggcaa
    58021 atccagcatc accgactgca tgatcatctg tacgggtacg tccagccgtc atgttatgtc
    58081 cattgctgac cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga
    58141 aggtgaaaac agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat
    58201 gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct
    58261 gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta
    58321 cctgcgtcgt tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggcaaacg
    58381 cggcaagaat gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc
    58441 cgcaggcaaa aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca
    58501 gttagccgct gagctggaac gctggaagct ggatggtcgc gacgtaagtc tactgattgg
    58561 cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggct gagcagagct ggtcgctgtc
    58621 ggcgcttacc ctcccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc
    58681 gtggagcatc accaccaacc atccttatca ccgtgagtga taagggagct ttgagtagaa
    58741 aacgcagcgg atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt
    58801 tgtgcgccgg gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc
    58861 caacctgtat aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa
    58921 ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt atctacgacc gtaacggtat
    58981 ccctctggcc ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa
    59041 cgtgcagcaa acgctggacg ctttgcgcag cgtggtagat ctgaccgatg acgatattgc
    59101 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa
    59161 cctaaccgaa gtacaagtag ctcgctttgc cgtcaatcag taccgttttc cgggtgtcga
    59221 agttaaaggc tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg
    59281 ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc
    59341 caactatgcg gcaacgcatg atattggtaa gctgggcatt gagcgttact atgaagacgt
    59401 gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg
    59461 ccagttaaaa gaagtaccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa
    59521 actccagcaa tatattgaaa cgctgctggc gggtagccgc gcagctgtgg ttgttaccga
    59581 accgcgtaca ggtggggtgc tggcgctggt ttccacgcct agttatgacc caaacctgtt
    59641 tgttgacggt atctccagca aagattattc cgccttgctg aacgacccga atacaccgct
    59701 ggtgaaccgc gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgtggc
    59761 ggtttcggca ttaagcgccg gggtgatcac gcgcaatacg acgctgtttg acccaggctg
    59821 gtggcaacta ccagggtcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg
    59881 tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
    59941 cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac
    60001 cggtatcgac ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa
    60061 acgctttaaa aaaccgtggt atcagggtga caccattccg gttggtatcg gccagggtta
    60121 ctggacagcg accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat
    60181 cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt
    60241 acaaccgcat gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga
    60301 cggtatgtac ggtgttgcta accgccctaa tggtacggcg aataaatact ttgccagcgc
    60361 accgtacaaa attgcggcga aatctggtac cgctcaggtc ttcggtctga aagcgaacga
    60421 aacctataat gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt
    60481 tgcgccatac aacaatccgc aagtggctgt cgccatgatt ctggagaacg gcggcgcggg
    60541 tccggcggtt ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa
    60601 caccgatctg cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg
    60661 gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg
    60721 attttactgg cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat
    60781 attggcatga tggagcgtaa aatcggccaa atcgcgatgg gtctggtcat catggtggtg
    60841 atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt
    60901 attattttgc tggtggcggt agatgctttc ggagccatct ctaaaggcgc tcaacgctgg
    60961 ctggacctcg gtattgttcg ttttcagccg tcggaaattg ccaaaatagc cgtaccactg
    61021 atggttgcgc gctttatcaa ccgcgacgtt tgcccgccat cgttgaagaa tactggcatc
    61081 gcgctggtgc tgatatttat gcccacgctg ctggtggctg cacagcctga cctgggaaca
    61141 tcaatcctcg ttgcgctttc cggtctgttt gtactgttcc tctctggcct tagctggcgt
    61201 ctgattggcg tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg
    61261 atgcatgatt accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc
    61321 ggcgcgggct atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc
    61381 aaaggctggc tgcacggcac tcagtcacag cttgaatttc tccccgaacg ccatactgac
    61441 tttatcttcg cggtactggc ggaagagctg ggattagtgg gcattctgat tctgctcgct
    61501 ctctacattc tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt
    61561 ggtcgcgtca tggctggtgg cttaatgctg atattattcg tttatgtctt cgtaaatatt
    61621 ggtatggtaa gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga
    61681 ggatcggcgc taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac
    61741 aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat
    61801 ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt
    61861 aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcggaccc
    61921 gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta
    61981 caaaatcgtg caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc
    62041 cgaaccaggc agtaacctga cggcctctgg cgaagctttc gatccgacaa agctgacggc
    62101 ggcccatcca acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg
    62161 aatgatcgtg gtgcgcatta atgatcgcgg cccttacggc aacgaccgcg ttatttcgct
    62221 ttctcgcgcg gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc
    62281 gattattgtc gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc
    62341 caaacagact tacgccctgc ctgcacctcc cgatttaagc ggtggcgcgg gaacaagttc
    62401 agtgtctggc ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga
    62461 tccgaccggc gcgccggtaa ccagtagcgg tttcctcggc gctccaacga ccttagcgcc
    62521 tggtgtactg gaaggcagcg aaccgacgcc tgcgccacag cccgttgtta cagcttcgtc
    62581 gacaacgcct gcaacctcgc ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag
    62641 cggcaacttt atggtgcaag tcggggccgt aagcgatcag gctcgtgcgc aacagtacca
    62701 acagcaactg ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg
    62761 gcggatccag cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt
    62821 gcaaaccgaa gcccaattac agtcatttat tactaccgcg cagtagcgta aagcaggcat
    62881 ctgatgtgtc aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc
    62941 cggtagtgca tttgctatag tagggcactt ttttaattcc atcacggatg tcgtagttca
    63001 gaccatgaat accatttttt ccgctcgtat catgaagcgc ctggcgctca ccacggctct
    63061 ttgcacagcc tttatctctg ctgcacatgc cgatgacctg aatatcaaaa ctatgatccc
    63121 gggtgtaccg cagatcgatg cggagtccta catcctgatt gactataact ccggcaaagt
    63181 gctcgccgaa cagaacgcag atgtccgccg cgatcctgcc agcctgacca aaatgatgac
    63241 cagttacgtt atcggccagg caatgaaagc cggtaaattt aaagaaactg atttagtcac
    63301 tatcggcaac gacgcatggg ccaccggtaa cccggtgttt aaaggttctt cgctgatgtt
    63361 cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc cgcggtatta acctgcaatc
    63421 gggtaacgat gcttgtgtcg ccatggccga ttttgccgct ggtagccagg acgcttttgt
    63481 tggcttgatg aacagctacg ttaacgcact gggcctgaaa aatacccact tccagacggt
    63541 acatggtctg gatgctgatg gtcagtacag ctccgcgcga gatatggcgc tgatcggcca
    63601 ggcattgatc cgtgacgtac cgaatgaata ctcgatctat aaagaaaaag aatttacgtt
    63661 taacggtatt cgccagctga accgtaacgg cctgttatgg gataacagcc tgaatgtcga
    63721 cggcatcaaa accggacaca ctgacaaagc aggttacaat cttgttgctt ctgcgactga
    63781 aggccagatg cgcttgattt ctgcggtgat gggtggacgt acttttaaag gccgtgaagc
    63841 cgaaagtaaa aaactgctaa cctggggctt ccgtttcttc gaaaccgtta acccactgaa
    63901 agtaggtaaa gagttcgcct ctgaaccggt ttggtttagt gattctgatc gcgcttcgtt
    63961 aggggttgat aaagacgtgt acctgaccat tccgcgtggt cgcatgaaag atctgaaagc
    64021 cagctatgtg ctgaacagca gtgaattgca tgcgccgctg caaaagaatc aggtcgtcgg
    64081 aactatcaac ttccagcttg atggcaaaac gatcgagcaa cgcccgctgg ttgtgttgca
    64141 agaaatcccg gaaggtaact tcttcggcaa aatcattgat tacattaaat taatgttcca
    64201 tcactggttt ggttaaaaat tgaacacttg aaagtgtaat ttccgtcccc atatactaag
    64261 catcagtaaa aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga
    64321 gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa
    64381 gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat
    64441 gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta
    64501 tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc
    64561 aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg
    64621 ggtaactccc ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg
    64681 agatgccgtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga
    64741 gccaatctcc cgggctatgc atgaattcac cgatacccgc gataatagta cccttgatga
    64801 aatctggctg gtcgagcact atccggtatt cacccaaggt caggcaggaa aagcggagca
    64861 cattttaatg ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta
    64921 tcacgggccg ggacaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg
    64981 tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg
    65041 tatagaagcg catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg
    65101 ctcactgggt ttacgaattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa
    65161 tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgccggaa tggaaatggc
    65221 taaaatatca caatggaaac ccgaagcgac gactaataat attgctccac gtttactgga
    65281 aaatatttta gcgctactaa acaatccgga cttcgaatat attaccgctt aattccatac
    65341 atcaatggcc caatttacac tgggtcatta ctcataatac ctgctaatgc tttacatctc
    65401 cttcgcgttt gtatattttc tttatcactt gcagaaatgc ggtgccacgc cttgctccgt
    65461 aactcacccc attgattaac ctattgaaat agttgattat ggtgagcaaa agcgttccgt
    65521 tccaattaga cataagatta ttgtaatttt ttaaatatta tttttccatg aataaaattg
    65581 gagtaagtgc gtggatagta ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga
    65641 aggtaaacca caaatattta caaccttacg aaatattgat cttaaccttc tgactatttt
    65701 tgaagctgta tatgtacata aagggatcgt taatgcagcg aaagtgctta atctgacccc
    65761 ctcggcaatc agtcagtcta ttcagaaact gcgcgttata ttccctgacc cattgtttat
    65821 tcgcaaaggc cagggtgtca ctcctaccgc atttgcgatg catctacatg agtatatcag
    65881 tcagggcctt gagtccattc ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg
    65941 aacgataact attgcgacca ctccctcggt cggagccctg gtccttcctg tcatctatcg
    66001 ggcgattaaa actcactatc cgcagctttt actgcgcaac ccgcccatca gcgacgcgga
    66061 aaaccaactc agtcagtttc aaaccgatct catcatcgat aacatgtttt gcaccaatcg
    66121 tacggtgcaa catcatgttc tgttcaccga caatatggtg ttaatttgcc gcaagggaaa
    66181 tccactactc tctttagaag atgacagaga gactatcgac aacgctgcgc atgtacttct
    66241 gttaccggaa gggcaaaatt tcagcggtct gcggcagaga gttcaagaga tgtttccgga
    66301 ccggcaaatc aatttcacca gctacaacat tttgacaatc gctgcactgg ttgccaacag
    66361 tgacatgtta gcgattattc ccagccgttt ttataacctg tttagtcgtt gctggccgct
    66421 ggaaaaactg ccttttccgt ccttaaatga ggagcaaata gatttctcta tccactacaa
    66481 caaattcatc ctgcgtgatc ccatcctgca cggagtaata gatgttatcc gtaatgcatt
    66541 ttgaaaaagt attgttacag ccagatcaca gcgcagaacg ataaaatggc acaatccaac
    66601 aacaatttta cattttcgcg accgctttgg ctgctttcag gtccgtttca atgatatact
    66661 gccagtcgtt aattcaaaaa tagttgataa ttacaacaat ctattgaatt gaaacgcttt
    66721 ccttcgtaat tcgcaactgg aacacgcacg ctatgagtaa acccattgtg atggaacgcg
    66781 gtgttaaata ccgcgatgcc gataagatgg cccttatccc ggttaaaaac gtggcaacag
    66841 agcgcgaagc cctgctgcgc aagccggaat ggatgaaaat caagcttcca gcggactcta
    66901 cacgtatcca gggcatcaaa gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg
    66961 aagcctcctg ccctaacctg gcggaatgct tcaaccacgg cacagcaacg tttatgatcc
    67021 tcggcgctat ttgtacccgc cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag
    67081 ctcctgatgc caatgaacca gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt
    67141 atgtggttat cacctccgtt gaccgtgatg acctgcgcga tggcggtgcc caacactttg
    67201 cggattgtat tactgccatt cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc
    67261 cggatttccg cggtcgaatg gatcgtgctc tggatattct gactgcaacg cccccagatg
    67321 tgttcaacca taacctggaa aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag
    67381 attacaactg gtcgctgaag ctgctggaac gctttaaaga agcgcatccg gaaatcccga
    67441 ccaagtctgg tctgatggtg ggtctgggtg aaaccaatga agaaattatt gaggtaatgc
    67501 gcgacctgcg ccgtcatggt gtgacgatgt taacgctggg gcaatatttg cagccaagcc
    67561 gccatcacct gccggttcaa cgttacgtta gcccggatga gttcgacgaa atgaaagccg
    67621 aagcgctggc gatgggcttt acccatgctg catgcggtcc gtttgtccgc tcttcttacc
    67681 acgccgattt gcaggcgaaa ggaatggaag ttaagtaatc ctgtaataat tacttacatc
    67741 tgtctgataa aaaacccgct ttttgaagcg ggttttttgt atcgaacaag atattgaggg
    67801 agcgtcctgc tcgccacgtc actctttatg agagagcttt tcagcctgaa gatcaacgtc
    67861 tgcgcctttt ttcgccgcag catcgtcatc attcatcgcc ttcttgaacc ctttaatggc
    67921 cgctccaagg tctccgccca gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag
    67981 cgccgcaact accagcagtt tggtaatact aatctcaccc atagatacct tcttgacata
    68041 taaccggcaa cagaacgcgc cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc
    68101 acacaatagc aatctgtaac aaggtgaatc aagcgccgag taaaaaaaca tcataataat
    68161 tgcggcggcg caaagcgacg gttgtttaag atgggcagtt gcgcgcgcac ctgacgcaca
    68221 cgttcgggcg tcacttccgc cataatgagt gcaggcattt ctgacgctgc cgcaatggtg
    68281 acgccaaagg gatctataat ccggctttgc ccgatatttt tgttcccgca ctcccccgcc
    68341 gccaccatat aacaggtggt atccagcgca cgagcggcaa gcaacgttga ccagtgatgc
    68401 tctttgagcg gcccacgaac ccaggcggca ggaagtacca ggatttcagc tccctgtaaa
    68461 gcctgtgcca gcgccagctc tggaaagcgt aagtcataac aggtcatcag accgaccttc
    68521 atcccttcca cctccagtaa cggagctatt tcattaccag catcaacatg gcgtgattcc
    68581 tgaatggcaa atgcatcata gagatgcagt ttggcataac gggcgacgat gtttcctgcc
    68641 tgaagtgcca ccagcatatt ccatgcgcgc cccggcgttg aaggaagatg aatcgtcaga
    68701 attgtcgtca tcatgttacg tttactttct cgccgtaaac gtccgaggaa ttcgccttcc
    68761 agcagctgtg ctgatttaac cgatagatct gcatcatgat catcgcgcgc cagcaatgct
    68821 tccggcagga caaacagcga tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa
    68881 atctcagcgt tcttttccca cacagatgta acagcaaact gtcctgcagc aaccagcatt
    68941 gccacctcct gtcagagtcg taaaattcgc caacgacgat tattcggcgt tacacttatc
    69001 actcatacaa atcaaatagc aggattttgc agtgttacaa cttcttttag cagtttttat
    69061 tggcggtggt acgggaagcg tagcgagatg gctgttaagt atgcgattta acccgctgca
    69121 tcaggcgatt ccgttgggga cgctggcagc aaacctgatt ggggcattca tcataggaat
    69181 gggattcgcc tggttcagca ggatgacgaa cattgatcca gtgtggaaag tattaatcac
    69241 caccggattt tgtggcggtc taacaacctt ctcaacattt tcggcagaag tggtgttttt
    69301 gttacaagag ggccgctttg gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc
    69361 ttttgccatg accgcactgg cattctggct gttttcggcc tcaaccgtac actaaagacc
    69421 aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat
    69481 tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc
    69541 ttcagcaaga gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt
    69601 gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt
    69661 acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc
    69721 ggttatcagt acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata
    69781 aaagtcgcgt tatttttgat gttgcttatt ttagttattg attttaaaga agttactaaa
    69841 acttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta
    69901 ggtacccgga atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg
    69961 cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa
    70021 tcccagacct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta
    70081 tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg
    70141 ccagtttcct ttttcatccc aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt
    70201 atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat
    70261 atataaatca taatgttcag gctgttgcca ggtttgtgca atattttctc taaacgttgt
    70321 catcctctca tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa
    70381 ggaaaagata gcgacatatt tactcacgtt catttgtgac cataaaacat ttatcaaaaa
    70441 tctactacta gcatagcaaa gctacaatta acataacctt aatagaccca acataaagaa
    70501 taatctgaat agctgctcta tttatttccg cttgttcaag ttaaatattt ccgcgcatcg
    70561 attaaagatg atgcgtcgtt ttctgcgatg ggaatagtca aaaaagaaaa accaagtctt
    70621 ttttgatgac aaaatgcaat caaggaaaaa ttattttatt tttttaacga gatacaacaa
    70681 tcatcttaac gaagtatata atatatatcg tcaacccatg ctcatattag attatttttt
    70741 gagcgcacag ccgcagctaa caccattgca attaacaaat ttgcatcaat ccaccatcaa
    70801 tttacacaca ttattatgtg ataattgcca accgctaaat atgcgttttg ttatctatgt
    70861 ataaaaacag caacttcaat gtcttaatgg cagtttttct tgattttaat cagcattcat
    70921 cgccaattta ttgggcatat tttttcctta agctttagga agtttttatt atttactttg
    70981 ggcctggaga caggaaaaat tatgctgaca ttcattgaac tccttattgg ggttgtggtt
    71041 attgtgggtg tggctcgcta catcattaaa gggtattccg ccactggtgt gttatttgtc
    71101 ggtggcctgt tattgctgat tatcagtgcc attatggggc acaaagtgtt accgtccagc
    71161 caggcttcaa caggctacag cgccacggat atcgttgaat acgttaaaat attactaatg
    71221 agccgcggcg gcgacctcgg catgatgatt atgatgctgt gtggctttgc cgcttacatg
    71281 acccatatcg gcgcgaatga tatggtggtc aagctggcgt caaaaccatt gcagtatatt
    71341 aactcccctt acctgctgat gattgccgcc tattttgttg cttgtctgat gtcactggcc
    71401 gtctcttccg caaccggtct gggggttttg ctgatggcaa ccctgtttcc ggtgatggta
    71461 aacgttggta tcagtcgtgg cgcagcagct gccatttgtg cctccccggc ggcgattatt
    71521 ctcgcaccga cttcagggga tgtggtgctg gcggcgcagg cttccgaaat gtcgctgatt
    71581 gacttcgcct tcaaaacgac gctgcctatc tcaattgctg caattatcgg catggcgatc
    71641 gcccacttct tctggcaacg ttatctggat aaaaaagagc acatctctca tgaaatgtta
    71701 gatgtcagtg aaatcaccac cactgccccc gcgttttatg ccattttgcc gttcacgccg
    71761 atcatcggtg tactgatttt tgacggtaaa tggggtccgc aattacacat catcactatt
    71821 ctggtgattt gtatgctgat tgcctccatt ctggagttcc tccgcagctt taatacccag
    71881 aaagttttct ctggtctgga agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg
    71941 gtgatgctgc tggttgccgc tggggtattc gctcaggggc ttagcaccat cggctttatt
    72001 caaagtctga tttctatcgc tacctcgttt ggttcggcga gtatcatcct gatgctggta
    72061 ttggtgattc tgaccatgct ggcggcagtc acgaccggtt caggcaatgc gccgttttat
    72121 gcgtttgttg agatgatccc gaaactggcg cactcttccg gcattaaccc ggcgtatttg
    72181 actatcccga tgctgcaggc gtcaaacctg ggtcgtaccc tatcacccgt ttctggcgta
    72241 gtcgttgcgg ttgccgggat ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg
    72301 gtgccggtgc ttgttgggct agtgattgtt atcgttgcta cagagctgat ggtgccagga
    72361 acggcagcag cggtcacagg caagtaaata gtaatgccgg aggcaagttt cctccggctt
    72421 tatcatgtca cccactgtgg tatatgcgtt gtggtctgcc aactttgccg tgaacaattt
    72481 cagcaataat cagatggcgg ctggcgcaat attcaagata acgcctggca gtggtgcggc
    72541 tgatggtcag tgcctgcgcc accgtttctg ccgtatgttg cacaccaggc tctttaaaca
    72601 gttttcgcac cgcgtttagc gtcaaggggt caatgccggt cggcagttcg tctttgggtt
    72661 caccgcgagc ataggcatta aacatctcat caatttgctt ctggctggcg ctatcaatac
    72721 tttccagcat atgtttacgc tggcggaaac gggttagcgt ttgccccagt gtattgattc
    72781 acttgaagta cgaaaaaacc gggagaacat tggattattc gggatctgat gggattagat
    72841 ttggtggggc ttgcaagcct gtagtacaaa ttttaggcgt taaccaatga aacgcgaaag
    72901 atagtaaaaa attgcttttg tttcattgaa aaaacgaaaa acaaaaacac tataaatcat
    72961 ttcaatcata gcttcaaaaa acgttcaaaa ccgataacaa ccaggctgtc accaaatgac
    73021 tcatatcaca aatcagctca tgccgtttag gtatgttaca tgtgtgatta tgtgaggtga
    73081 agtatgtttt agctggctca tggttgttat acggcttttt tcgcatcttg tggttccaac
    73141 aaaggagcta caggctcttc ctgtttatga acaccataca ttgacaaaat ctcttggcga
    73201 gtttctggag gtaatttatg cagcgccaaa gctgtttgta gtgtttggcg atcaataccc
    73261 agcgttgcca atagggtgtc aacggagatg gaggggcgtt cttgcattaa tccggaaagc
    73321 ccatatttag ccatgtttag ctctatctct cggacttcat tttctcctag tttattgatt
    73381 aacagggaga acattttcaa tgtatcagca ttcatttctt cgatagcaat tttttgcata
    73441 gccggtattg ccgggatata ttcaacccca ggcccgcgac gcccttcttg tgaacgcttc
    73501 ggccatcctt ctttctttgc tcgtttatgc accccctgta ttgtcccagg cattccatcc
    73561 agttcagtaa tttgtttggg tgtcatccac tggtttatct catttgcgct tttcataaac
    73621 taagtttacc agatttatct atataaaaat caatggatta caaaataaac cacattaact
    73681 gatataaacc ggtttggttt atttggtaat atataaaaca gataatcaag acgaattatt
    73741 tgttttggta accctttatc atacgcaata tggttggtgt gcgaaagatg agtaacgatt
    73801 ggcatcgggc cgacgtgatt gcggccctca aaaaaacagg aacccctctg gcaaaattgt
    73861 ccagacgttc aggttattca accgggacgc ttgcaaatgc attagtcagg ccatggccta
    73921 aaggtgaacg cattattgct gacgccttaa acatgaaacc tgaggaaatt tggcctagta
    73981 gatatctaag ggatcacaat gagagagtgg atatcagcaa ttgaatgcat gggtttatct
    74041 ggtttacctg gttcattaat gggggttcac aaaagagctc agagagaagg atggctcaag
    74101 agaagaagag aaggaattag aggggttgct tacgaatacc atatatcttc tcttcctaac
    74161 attgctcgag ctgaagtttt gctccaacaa ggaaaattta aaaccagtca gggatgtttt
    74221 gaaatcgccc gccccacgct ggaagctcat gattatgatc gtgaggcact atggagcaaa
    74281 tgggataaag ccagcgattc ccagcgcagc ctggctgaaa aatggttgcc ctcaattcag
    74341 gccacagacg aaatgctgaa ccaggggatt tcaacgaaaa cggcttttgc gaccgttgca
    74401 gggcattacc aggtcagcgc atccactttg cgggacaagt attaccaggt acagaagttt
    74461 gcgaagcctg actgggcggc tgcacttgtt gatggacgtg gagcatcccg tcgcaatgtt
    74521 cacaaaagtg aatttgacga ggatgcctgg cagtttctga ttgcagatta tctgcgaccg
    74581 gaaaaacccg ccttccgcaa atgttatgag cgtctggaac tggcagcccg cgagcatggc
    74641 tggagtattc cctcccgtgc cacggccttt cgccggattc agcaactgga cgaggcaatg
    74701 gttgttgcct gtcgtgaagg tgaacatgca ctgatgcatc tgataccggc acagcagcga
    74761 actgtggaac acctggacgc catgcagtgg atcaacggcg acggttatct gcataacgtc
    74821 tttgtacgct ggtttaacgg tgatgtgatc cgtccgaaaa catggttctg gcaggatgtg
    74881 aaaacccgaa aaattctggg ctggcgctgc gatgtgagcg agaacattga ttcaattcgc
    74941 ctctcgttca tggatgttgt gactcgctac agtatcccgg aggattttca catcaccatt
    75001 gataacaccc gtggtgctgc gaataaatgg ctgacgggag gcgcgcccaa tcgctaccgc
    75061 tttaaggtaa aagaggacga tccaaaagga ctgtttttac tgatgggtgc gaaaatgcac
    75121 tggacaagcg ttgttgccgg taaaggctgg ggccaggcaa aacctgttga acgtgctttc
    75181 ggtgttggtg ggcttgagga atacgttgat aagcatccgg cactggctgg cgcatatacg
    75241 gggccaaatc cgcaggcaaa acctgataac tatggcgacc acgctgttga tgcagagctg
    75301 tttctgaaaa cctttgccga aggtgtggcg atgttcaatg ccagaacagg ccgtgaaaca
    75361 gaaatgtgcg ggggcaaact ctcgtttgat gatgttttcg agcgtgaata cgccagaacg
    75421 attgtgcgta agccaaccga agaacaaaaa
//
LOCUS       DAJTRD010000004        72967 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTRD010000004 DAJTRD010000000
VERSION     DAJTRD010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72967
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.004"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO315_000315"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO315_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5889359.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO315_000316"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO315_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5889360.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO315_000317"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO315_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5889361.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO315_000318"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO315_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5889362.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO315_000319"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO315_000319"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5889363.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO315_000320"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO315_000320"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5889364.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO315_000321"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO315_000321"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5889365.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO315_000322"
     CDS             complement(4598..5320)
                     /locus_tag="OO315_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5889366.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO315_000323"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO315_000323"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5889367.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO315_000324"
     CDS             6520..6738
                     /locus_tag="OO315_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5889368.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO315_000325"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO315_000325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5889369.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO315_000326"
     CDS             complement(7472..7672)
                     /locus_tag="OO315_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5889370.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO315_000327"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO315_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO315_000328"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO315_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5889371.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO315_000329"
     CDS             10101..12014
                     /locus_tag="OO315_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5889372.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO315_000330"
     CDS             12014..13036
                     /locus_tag="OO315_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5889373.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO315_000331"
     CDS             13030..13248
                     /locus_tag="OO315_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5889374.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO315_000332"
     CDS             13302..14171
                     /locus_tag="OO315_000332"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5889375.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO315_000333"
     CDS             complement(14226..14630)
                     /locus_tag="OO315_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5889376.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO315_000334"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO315_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5889377.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO315_000335"
     CDS             15615..17705
                     /locus_tag="OO315_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5889378.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO315_000336"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO315_000336"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5889379.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO315_000337"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO315_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5889380.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO315_000338"
     CDS             complement(19673..20275)
                     /locus_tag="OO315_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5889381.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OO315_000339"
     CDS             complement(20265..20432)
                     /locus_tag="OO315_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5889382.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO315_000340"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO315_000340"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5889383.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OO315_000341"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OO315_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5889384.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OO315_000342"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OO315_000342"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5889385.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OO315_000343"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OO315_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5889386.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OO315_000344"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OO315_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5889387.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OO315_000345"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OO315_000345"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5889388.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OO315_000346"
     CDS             28269..28436
                     /locus_tag="OO315_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5889389.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OO315_000347"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OO315_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OO315_000348"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OO315_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5889390.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OO315_000349"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OO315_000349"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5889391.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OO315_000350"
                     /pseudo
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OO315_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OO315_000351"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OO315_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5889392.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OO315_000352"
     CDS             complement(33764..34849)
                     /locus_tag="OO315_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5889393.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OO315_000353"
     CDS             complement(34861..36165)
                     /locus_tag="OO315_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5889394.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OO315_000354"
     CDS             complement(36177..36530)
                     /locus_tag="OO315_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5889395.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OO315_000355"
     CDS             complement(36541..37419)
                     /locus_tag="OO315_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5889396.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OO315_000356"
     CDS             complement(37416..38642)
                     /locus_tag="OO315_000356"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5889397.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OO315_000357"
     CDS             complement(38643..39806)
                     /locus_tag="OO315_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5889398.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="OO315_000358"
     CDS             complement(39890..40252)
                     /locus_tag="OO315_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5889399.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="OO315_000359"
     CDS             complement(40269..41174)
                     /locus_tag="OO315_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889400.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO315_000360"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO315_000360"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5889401.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO315_000361"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO315_000361"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5889402.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO315_000362"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO315_000362"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5889403.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO315_000363"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO315_000363"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5889404.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO315_000364"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO315_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5889405.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO315_000365"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO315_000365"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5889406.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO315_000366"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO315_000366"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5889407.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO315_000367"
     CDS             complement(48281..49519)
                     /gene="hofQ"
                     /locus_tag="OO315_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5889408.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO315_000368"
                     /pseudo
     CDS             complement(49431..49837)
                     /gene="hofP"
                     /locus_tag="OO315_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO315_000369"
     CDS             complement(49827..50267)
                     /gene="hofO"
                     /locus_tag="OO315_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5889409.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO315_000370"
     CDS             complement(50251..50790)
                     /gene="hofN"
                     /locus_tag="OO315_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5889410.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO315_000371"
     CDS             complement(50790..51569)
                     /gene="hofM"
                     /locus_tag="OO315_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5889411.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51689..54241
                     /gene="mrcA"
                     /locus_tag="OO315_000372"
     CDS             51689..54241
                     /gene="mrcA"
                     /locus_tag="OO315_000372"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5889412.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO315_000373"
     CDS             complement(54406..54966)
                     /gene="nudE"
                     /locus_tag="OO315_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5889413.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55286..57421
                     /gene="igaA"
                     /locus_tag="OO315_000374"
     CDS             55286..57421
                     /gene="igaA"
                     /locus_tag="OO315_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5889414.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57486..58154
                     /gene="yrfG"
                     /locus_tag="OO315_000375"
     CDS             57486..58154
                     /gene="yrfG"
                     /locus_tag="OO315_000375"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5889415.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58165..58566
                     /gene="hslR"
                     /locus_tag="OO315_000376"
     CDS             58165..58566
                     /gene="hslR"
                     /locus_tag="OO315_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5889416.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58591..59460
                     /gene="hslO"
                     /locus_tag="OO315_000377"
     CDS             58591..59460
                     /gene="hslO"
                     /locus_tag="OO315_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5889417.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59604..61355)
                     /locus_tag="OO315_000378"
     CDS             complement(59604..61355)
                     /locus_tag="OO315_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5889418.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61734..63356
                     /gene="pckA"
                     /locus_tag="OO315_000379"
     CDS             61734..63356
                     /gene="pckA"
                     /locus_tag="OO315_000379"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5889419.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="OO315_000380"
     CDS             complement(63432..64784)
                     /gene="envZ"
                     /locus_tag="OO315_000380"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5889420.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="OO315_000381"
     CDS             complement(64781..65500)
                     /gene="ompR"
                     /locus_tag="OO315_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5889421.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65728..66204
                     /gene="greB"
                     /locus_tag="OO315_000382"
     CDS             65728..66204
                     /gene="greB"
                     /locus_tag="OO315_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5889422.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66301..68622
                     /locus_tag="OO315_000383"
     CDS             66301..68622
                     /locus_tag="OO315_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5889423.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69060..69287
                     /gene="feoA"
                     /locus_tag="OO315_000384"
     CDS             69060..69287
                     /gene="feoA"
                     /locus_tag="OO315_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5889424.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69304..71625
                     /gene="feoB"
                     /locus_tag="OO315_000385"
     CDS             69304..71625
                     /gene="feoB"
                     /locus_tag="OO315_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5889425.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71625..71861
                     /gene="feoC"
                     /locus_tag="OO315_000386"
     CDS             71625..71861
                     /gene="feoC"
                     /locus_tag="OO315_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5889426.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72064..72951
                     /gene="rpnA"
                     /locus_tag="OO315_000387"
     CDS             72064..72951
                     /gene="rpnA"
                     /locus_tag="OO315_000387"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5889427.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAAASRLAP"
BASE COUNT        17586 a        19773 c        18455 g        17153 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa agggcgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggtttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgtgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctag gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgcgccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaaatt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgggtaagat tagcgtaaaa
    48121 aagacagcaa aatgccgcct gaatgataaa tcatcatcat ggaacgcagg cagcgaagag
    48181 caccaactct caaatcggta ctccttgtat gctaaatacc tgcgcgcgtc aaatagatga
    48241 aacacgttca gcataaaaac attaccgctt ttacggctgt ttactcactg gaaaccagtc
    48301 gtggcgtgat aaacaccact aactcgcgtc gttcatcttc ttttccgtca tgacgaaata
    48361 attgcccgaa ccagggaatg tcgccaagca acggtacgct atcctgaccc gatttatttt
    48421 tacgggtaaa aatgccgccc agcgccaacg tttctccgct ttttacctcg acctgcgttt
    48481 cgatctcctg cttatcaatc gccagcactt cgccatcggc ctgctgtagc acctgccccg
    48541 gaacgttctg gctgatgtgt aatttcagcc ggatgcgacc tttttgtaac accgtgggcg
    48601 tgacctccat ccccaggacg gcctctttaa attccaccga cgtcgcgcca ctttccccgc
    48661 tggaaacctg atatggaatt tcgctctcct gtttaatgct ggcaggctga agatgtgagg
    48721 ccagcagacg cggactggcg ataatatcca gctgctgttt ttgttcgagc gcggaaagct
    48781 caagatccag caagcgtccg ttgatgcgcc caatgttaaa accgacatgc gttgtcgccg
    48841 tcgctacgga gaggtcgcta ccaagcgtgg tgacttgccc aacgccacca gcgtgttgcg
    48901 catcggccag cgtccatttc acgcctaact cacgcaaact tttttcatta atggtgacaa
    48961 tatgcgccga cagctcaacc tgcccgaccg gcagatccat ttgcgctacc cactgttcaa
    49021 gcgcgcttaa cgccgttttg ttatctcgta gcaaaaggcg attggtgcgt ttatcgacgg
    49081 tcatactccc tttggcactc agtagcttct cccccgcttt cgccagttct cccgcgtcgg
    49141 cgtattgcag ggttatactg cgattttcca gcggcagatt tgcctgcgcc cgcgcctgct
    49201 ccgcctcctg gcgggcgata ttgtcattct gccaggcaat ggaatgcact gagagaatgt
    49261 tgccttcctg ccgcgttatc agtccggcgc ttttcactac agtttgtagt gcctgcttcc
    49321 agggaacatc tgttagatgt aacgacaccg taccgctgac gtctggcgac acgaccaggt
    49381 tcaacttctc ctgttcagcc agcgcctgca acacctgagc taccggaacg tcatccacca
    49441 tcagcgtcac tttttgcggc tttgccgcct gtacgccggg tatcagcatc aacagtagtg
    49501 cggctatcca ttgcttcatt tgtatctcct tgccgttgcc acaaccattg tggcggttcg
    49561 cagtttgtcc cggtacccag cgttagtacg tctggcgtca gctgtaaaat tgtccagccg
    49621 ttttccagca catcgttttg ctgcacccgt cgccatttct tttgcccgtc ttttattaca
    49681 ccgatgatgc gctcgcctcg ccctaccatc ccctgatagc gccactggct aagttcgcta
    49741 atccggcata tagatcttcc ggcgatttaa aagggtcacg cataccggtt aaaaggcaca
    49801 atgcaatacc tgccaacaac cagcgtttaa ccctcatgcg gcatctccag ttgtagcgtg
    49861 aacaaaagat catcaccttc cacgcttaac gaaaaacggc tcacgctgac gttgcgctct
    49921 gccagccgtg taaatgccga cggcactgct tcccacagcg ttttcaacgc caactcacct
    49981 ccctgcgcgg atggatgcca ggaaaccagt tgcgcgccgg ataactgaaa atccagtggc
    50041 gaaaagggca gcgttttttc ctcgctgaag ggagtggtgt ctaccaggcg atacagtgcg
    50101 gcccactgct ggtgatgact tgcccgtagt cgaattaatg cgtcgcgctc ttcatggtgt
    50161 gtcgatgata gaaaaatgag cgtaactaac atcagcaacc agaatgccca gcaaaactgg
    50221 cggaggcggg gtgatgtggc gaaccaccag tcaaagaaca tgttcatcgc taaccttcct
    50281 tgttaactga tactcaaatt gccagcgtcc ctgcgcatcc tgctgcgtgg ctccccgctg
    50341 attgagatga aaagacgcat cctggcggag tgacgtttct agtgcgttta acgcggtaat
    50401 gcttgttgtc agccccttga tctccagcgt tccctgctgc cagcttatcg ttgtcagcca
    50461 ggcgtgctcg ggtaaaagcg ccgccagtgc ttccagcgca gattgccagt cgcgggtaaa
    50521 ttgtcgctgg cgctgccgtt gcgaacgctg ttcgcgtaac tgctgttgct ccagcaaacg
    50581 tggcttcgtt atctgtaagc tgcgggcgag ttgttgttcc gcctgaagca aaactgcgtc
    50641 tatgcgcgct tcggcgctgc ctgtcagacg cagtattagc gttatcccga cggccagcag
    50701 cagaggcgca acgaacatca gcaaccagaa acgcagaaaa gcggtccggc gttgctgtcg
    50761 ccagggcaaa aaattaattg gcggattcat cagtacgcct tcccaagcgc cagccccagc
    50821 gcgatggcaa agtctccacc aggcggcggt agcggcggct gacgaacaga aacggcctcc
    50881 caggggtcaa atccgccttc gccacatatc gcgacgctgt cgggatcaac ggataacgct
    50941 gccgccagct ccttcgcgct agtcatcccc accgccagtt tgcgccccca gctatagcgc
    51001 gtcgcccaca gccactgttc gttatcacgc caggccagac attgctgatg agaaggtaaa
    51061 aaaggcagga atcgctgtaa tgcactggca tccggggtga tcgcactaac atgaacacgc
    51121 aacctttctg ccagcgtaag cagcgttgcc agctctttgc tttgcgcggc agtcacgtta
    51181 taagcggggc tgagtgagtc ttcgctgtaa tcgaagcgca gggagtccgg atccatatcc
    51241 agctcgcggg ccatcgtccc tgacagccag gccgtttgct cccgctcacc aagggacatc
    51301 gacgggcgcg gaaatgaccg ctgtaatgtg cgactggcgg gaaacgccaa cataatgtga
    51361 tgacgctgcg gcagttcgcg actccacggt aacaacgttt tagccagctg ctgcgcatca
    51421 acaatccgcc catctttgat aatgtcgtgc gccagcggca accgccacca gcgttgcaaa
    51481 aagcattctt ttgcgccccg cacgatcgca accgctgccg cttcttgctg ttgtaaatgc
    51541 aaaccaattt gccagatctt aaatgtcatt gtgatgatct ccttatcacc cgtcactctg
    51601 acgggtatat caatgcgtct ggcttgcctt tatactaccg cgcgtttgtt tataaactgc
    51661 ccaaatgaaa ctaaatggga aatttccagt gaagttcgta aagtattttt tgatccttgc
    51721 agtctgttgc attctgctgg gagcaggctc gatttatggc ctataccgct acatcgagcc
    51781 acaactgccg gatgtggtga cattaaaaga tgttcgcctg caaattccga tgcagattta
    51841 cagcgccgat ggcgagctga ttgctcaata cggtgagaaa cgtcgtattc cggttacgtt
    51901 ggatcaaatc ccaccggaga tggtgaaagc ctttatcgcg acggaagaca gccgcttcta
    51961 cgagcatcac ggcgttgacc cggtggggat cttccgtgcg gcaagtgtgg cgctgttctc
    52021 cggccacgcg tcacaagggg cgagtaccat tacccagcag ctggcgagaa atttcttcct
    52081 cagtccagaa cgcacgatga tgcgtaagat taaggaagtc ttcctcgcga ttcgcattga
    52141 acagttgctg acgaaagacg aaatcctcga gctttatctg aacaagattt accttggtta
    52201 ccgcgcctat ggtgtcggtg ctgcggcaca agtctatttc ggtaaaacgg tcgaccaact
    52261 gacgctgaac gaaatggcgg tgatagccgg gttgccgaaa gcgccttcca cctttaatcc
    52321 gctctactca atggatcgtg ccgtcgcgcg gcgtaacgtc gtactgtcgc ggatgctgga
    52381 tgaagggtat atcacccagc aacagttcga tcagacacgc actgaggcga ttaacgctaa
    52441 ttatcacgcg ccggagattg ctttctctgc gccgtacctg agcgaaatgg tgcgccagga
    52501 gatgtataac cgttatggcg aaagtgccta tgaagacggt tatcgcattt acaccaccat
    52561 cacccgcaaa gtgcagcagg ccgcgcagca ggcggtacgt aataacgtgc tggactacga
    52621 catgcgccac ggctatcgcg gcccggcaaa tgtgctgtgg aaagtgggcg agtcggcgtg
    52681 ggataacaac aagattaccg atacgctgaa ggcgctgccg acctatggtc cgctgctgcc
    52741 tgccgcagtc accagcgcca atcctcaggg agcgacggcg atgctggcgg acgggtcgac
    52801 cgtcgcattg agtatggaag gcgttcgctg ggcgcgtcct taccgttcgg atactcagca
    52861 gggaccgacg ccgcgtaaag tgaccgatgt tctgcaaacg ggtcagcaaa tctgggttcg
    52921 tcaggttggc gatgcatggt ggctggcaca agtgccggaa gtgaactcgg cgctggtgtc
    52981 gatcaatccg caaaatggtg ccgttatggc gctggtcggt ggctttgatt tcaatcagag
    53041 caagtttaac cgcgccaccc aggcactgcg tcaggtgggt tccaacatca aaccgttcct
    53101 ctacaccgcg gcgatggata aaggtctgac gctggcaagt atgttgaacg atgtgccaat
    53161 ttctcgctgg gatgcaggtg ccggttctga ctggcagccg aagaactcac caccgcagta
    53221 tgctggtcca attcgcttac gtcaggggct gggtcagtcg aaaaacgtgg tgatggtacg
    53281 tgcaatgcgg gcgatgggcg tcgactacgc tgcagaatat ctgcaacgct tcggcttccc
    53341 ggcgcaaaac attgtccaca ccgaatcgct ggcgctgggt tcagcgtcct tcaccccaat
    53401 gcaggtggcg cgcggctacg cggtcatggc gaacggcggc ttcctagtgg acccgtggtt
    53461 tatcagcaaa attgaaaacg atcagggcgg cgtgattttc gaagcgaaac cgaaagtagc
    53521 ctgcccggaa tgcgatattc cagtgattta cggtgatacg cagaaatcga acgtgctgga
    53581 aaataacgat gttgaagatg tcgctatctc ccgcgagcag cagaatgttt ctgtaccaat
    53641 gccgcagctg gagcaggcaa atcaggcgtt agtggcgaag actggcgcgc aggagtacgc
    53701 accgcacgtc atcaacactc cgctggcatt cctgattaag agtgctttga acaccaatat
    53761 ctttggtgaa ccaggctggc agggtaccgg ctggcgtgca ggtcgtgatt tgcagcgtcg
    53821 cgatatcggc gggaaaaccg gtaccactaa cagttcgaaa gatgcgtggt tctcgggtta
    53881 cggtccgggc gttgtgacct cggtctggat tggctttgat gatcaccgtc gtaatctcgg
    53941 tcatacaacg gcttccggag cgattaaaga tcagatctca ggttacgaag gcggtgccaa
    54001 gagtgcccag cctgcatggg acgcttatat gaaagccgtt cttgaaggtg tgccggagca
    54061 gccgctgacg ccgcctccgg gtattgtgac ggtgaatatc gatcgcagca ccgggcagtt
    54121 agctaatggt ggcaacagcc gcgaagagta tttcatcgaa ggtacgcagc cgacacaaca
    54181 ggcggtgcac gaggtgggaa cgaccattat cgataatggt gaggcacagg aattgttctg
    54241 attaaaaagg cgcttcggcg ccttttcagt tggctgacaa agtgcatttg tttatgccgg
    54301 atgcgggtga acgcgttatc cggctaacaa aatcgtgaaa aatcaatata ttgcaggaac
    54361 cccataagcc tgataagcgt tgtgcatcag gcaaacttca cgcatttaca ctcgcccctg
    54421 ccctttcaac cattcgcgca cgaggaacag cgcgctgaca ttacgcgctt cgttgaagtc
    54481 agggtcttcc agcaaatcca tcatatgcgc cagcggccag cgcacctgtg gtagcggctc
    54541 tggctcatcg ccttccagcg attccgggta gagatcttgc gcgaccacga tattcatttt
    54601 gctggaaaag taagacggtg ccatgctgag cttcttcaaa aaagtcagat cgttcgcacc
    54661 aaatccaacc tcttctttta gctcgcggtt agcggcttcg tagacgcttt cacccggatc
    54721 aattaatcct ttcgaaaaac ctaattcgta ggattcagtt cccactgcgt attcgcggat
    54781 caggatcagg tgatcgtcca caatcggcac aatcatcact gcttcccggt tggttggacg
    54841 cattcgttca taaacacgcc gcacgccatt gctgaactcc agatccacgc tctcgacggt
    54901 aaacagtcgg gaacgggcta cagtttcaac attcagaatg gtgggttttt gtaatgattt
    54961 gttcatcgtg ggatctatgc tgtgaaatca gcggttattg tgcgatatcg gacacgcttt
    55021 cggcaatgtg aattgcatgt tatttacatt tatgtaactt aataaataat cgtcctcaaa
    55081 tcaaattaaa agtcaatagg ttgaaataac tccaggaatt tgctgatatt ccgccttcag
    55141 agggtttgct atgatcagcg gttactgtga tgtgcttaat gatgctcaag ttaagctcca
    55201 cgcttgccga tagccaaccg cagaatcatg tattgtgtcc ggtgcgactg accacgcctg
    55261 acagactaag taagatgggg aaagcatgag caccattgtg atttttttag ctgctttgct
    55321 ggcctgctca ctacttgcgg gatggctgat aaaagtgcga tccagacggc gtcagctgcc
    55381 ctggaccaac gccttcgcgg atgcgcaaac gcgtaaactc acacctgaag aacgtagcgc
    55441 cgttgaaaat tatcttgaga gcctgacgca ggtattacag gtgcctggcc caacggaagc
    55501 tagcgcggca ccgatctctc tggcgctgaa tgccgaaagc aacaacgtca tgatgctgac
    55561 acacgctatc acacgttacg gcatctctac cgacgatccg aataaatggc gttactacct
    55621 cgattcggta gaagtccacc tgcccccctt ctgggaacag tacatcaacg atgagaatac
    55681 cgttgaactg attcataccg attcgctgcc gttggttatt tcactcaacg gtcatacgct
    55741 gcaggagtac atgcaggaaa cgcgcggcta tgccttgcaa cctgttccgt ccacgcaggc
    55801 gtcgattcgc ggggaagaga gtgagcaaat cgagctactc aatattcgca aagaaacgca
    55861 tgaagaatat gcgctgagtc gtccgcgcgg gctgcgtgaa gcgttgctga tcgtcgcctc
    55921 cttcctgatg ttctttttct gcctgattac cccggatgta tttgttccgt ggctggcagg
    55981 cggcgcatta ctgctgctgg gcgcaggtct gtgggggcta ttcgcgcccc cggcaaaatc
    56041 ctccctgcga gaaattcatt gtctgcgcgg tacaccccgt cgttggggat tgtttggcga
    56101 aaacgatcag gaacagatca acaatatttc actcggtatt atcgacctgg tctatcccgc
    56161 acactggcag ccatacattg ctcaggatct cggtcaacaa accgatattg atatctatct
    56221 cgaccgccac gtagtgcgtc aggggcgata tctttcgctg catgatgaag taaaaaactt
    56281 tccgttacag cactggctgc gcagtacgat tatcgctgcg ggctcgctgc tagtgctgtt
    56341 tatgctgtta ttctggatcc cgctggatat gccgctgaaa ttcactctct catggatgaa
    56401 aggcgcgcag accattgaag ccaccagcgt aaaacaactg gctgacgctg gcgtgcgggt
    56461 aggcgataca ttgcgtatta gcggtacggg aatgtgtaat attcgcactt ccggaacctg
    56521 gagcgcgaaa accaattcac cttttttacc gtttgactgc tcgcagatca tctggaatga
    56581 cgcccgctca ttaccattac cagaatctga actggtcaac aaagcgacgg cattgactga
    56641 agcagttaat cgccagctgc accctaaacc ggaagatgaa tctcgcgtca gtgcctcatt
    56701 acgttcagcg attcaaaaat ccggcatggt attgcttgat gattttggcg acattgtact
    56761 gaagacagcg gatttatgtt ctgccaaaga tgactgtgtg cgactgaaaa atgcgctggt
    56821 caatctcggc aacagtaaag actgggacgc gctggtaaaa cgcgccaacg ccgggaagct
    56881 cgatggcgtg aatgtgttat tacgcccggt gagtgctgaa tcgctggata acctggtggc
    56941 aacctccacc gcgccgttta tcacgcatga aacggcgcga gcggcacaat cactaaacag
    57001 tccggccccc ggcggattcc tgattgtcag cgacgaaggc agcgattttg ttgatcagcc
    57061 ctggccttcg gcatcacttt acgactaccc gccgcaagaa cagtggaacg ctttccagaa
    57121 actggcacaa atgctgatgc atacgccgtt taacgccgaa ggtatcgtca caaaaatctt
    57181 cactgacgcc aatggtacgc agcatattgg ccttcatccg atcccggatc gttccggcct
    57241 gtggcgctat ctcagcacca cattgctgct actgacgatg ctgggtagcg ccatttacaa
    57301 tggcgtacag gcctggcgtc gttaccagcg tcatcgcact cgcatgatgg agattcaggc
    57361 ctattatgaa agctgcctga acccgcaact gatcacccct tcagaaagcc ttatcgaata
    57421 acacgtttgc gcggcaggtt atgctaccct gtcgcgcaaa ttgcttcact ctggagattt
    57481 ccctcatgca tatcaacatt gcctggcagg acgtagatac cgttctgctg gatatggacg
    57541 gcacgttgct cgacctcgcc ttcgataact atttctggca aaagctggtg cctgaaacat
    57601 ggggcgcgaa aaacggggtt acgccacagg aagcgatgga atatatgcgc cagcaatatc
    57661 acgacgtaca gcatacgcta aactggtact gtcttgatta ctggagtgag caactgggtc
    57721 tggatatctg tgcgatgacc accgagatgg gaccgcgcgc cgtactgcgt gaagatacca
    57781 ttccgtttct tgaggcactg aaagccagcg gtaagcagcg aattttgctc accaatgctc
    57841 atccgcacaa cctggcggta aaacttgagc atacgggtct ggacgcacac cttgatttat
    57901 tactttccac ccacacattt ggttatccga aagaggatca gcggttatgg catgcggtgg
    57961 ccgaagctac gggtctgaaa gctgaaagaa cgctgtttat tgatgacagc gaagcgattc
    58021 tcgatgctgc cgcgcaattt ggtattcgtt actgcctcgg cgtgactaat cctgattccg
    58081 ggattgccga gaaacagtat caacgccatc cgtcactgaa tgactaccgc cgcctgatcc
    58141 cctcgctaat gtgaaggaga cgccatgaaa gagaaacctg ctgttgaggt tcgactggat
    58201 aaatggctat gggctgcccg tttttataaa acccgcgcgc tggcccgtga aatgattgaa
    58261 ggcggtaagg tgcattacaa cgggcagcgc agcaagccga gcaaaatcgt cgagctgaat
    58321 gccacgctca ctctgcgcca gggaaatgac gaacgcacgg tgattgtaaa ggcgattact
    58381 gaacagcgtc gccccgccag cgaggcagcc ttgctgtatg aagagactgc ggaaagtgta
    58441 gagaaacgcg aaaaaatggc gatggcacgt aaacttaatg ccttaaccat gccgcacccg
    58501 gaccgacgcc cggacaaaaa agagcgccgc gacctgttac gatttaaaca cggcgacagt
    58561 gaataactgt cacctgcaag agagatgatt atgccgcaac atgaccaatt acatcgctat
    58621 ctgtttgaaa attttgccgt tcgcggcgaa ctggtaaccg tttcggaaac cctgcaacag
    58681 atccttgaga accacgatta tccgcagccc gttaaaaacg tgctggcaga actgctggtt
    58741 gcgaccagcc tgttaaccgc tacgctgaag tttgatggtg atatcaccgt acagctgcag
    58801 ggcgacggtc cgatgaatct ggcggttatt aacggtaaca ataaccagca aatgcgcggt
    58861 gtggcgcgcg tgcagggcga aattccagaa aatgccgacc tgaaaacgct ggtcggcaat
    58921 ggttacgtgg tgatcaccat taccccgagc gaaggcgaac gctatcaggg cgtagttggt
    58981 ctggaaggtg ataccctggc ggcctgccta gaagattact ttatgcgttc tgaacagctg
    59041 ccgacgcgcc tgtttattcg caccggcgac gtagacggca aaccggctgc aggcggtatg
    59101 ttgttgcagg taatgcctgc gcaaaatacc caacaggacg actttgacca cctggcgacg
    59161 ctaaccgaaa ccatcaaaac cgaagaactg ctgaccttac cggcaaacga agtgttgtgg
    59221 cgtttgtatc acgaagaaga ggtgacggtt tacgatccgc aggatgtgga gttcaaatgc
    59281 acctgctcgc gtgaacgttg cgccgatgcg ctgaaaacgc tgcctgatga agaagttgat
    59341 agcatcctgg cggaagatgg cgaaattgac atgcattgtg attactgcgg taaccactat
    59401 ctgttcaatg cgatggatat tgctgaaatc cgcaacaacg cgtctccggc agatccgtaa
    59461 gttcattaat gagtatgtcc ggcagagagg gctctgccgg attttacgac ttaatggcct
    59521 gatgtgctgc gcttaccagc gcctatgaaa taaatcgcaa attattttgg tcagatacag
    59581 cgtcgtatcc gataaaagcg ttgtcactta cttaagttta tttccagcgt ttcaccatct
    59641 accgtcagat cgcgccacgc tttcggcctc gtccccagtt ttccatcctt cgcagctgtg
    59701 agtaattttt ctttcgttat ttcgttcgga agtaaactca tatcaagcgc gtcccattct
    59761 tttctgtctg agtcaaagcc atagacaatg actctgtcat cattaaaagc aaacaggatc
    59821 cgctccgcct gaccatcact attcagatct tgctcgacca gtacgcaggc gtctttttca
    59881 atacaggtca tcacgttata gcgatcctgg attaaggccg accagaatgt cgcatccggt
    59941 ttaacagaac caggggcaat taacacattt tcggcgaata ctttttccga tacctgttcc
    60001 tgaagatgtt gctctccatc taacgccatc agcagatccc gcgcgcgttt cgggtctttc
    60061 ataaacccgg catcgctttt cagcgactca agcgccgcac gtccatagcg accgctctgt
    60121 tcgagcatgt agatagttac ctggtctggc gtgtttttgc cgctctgata acgcgccata
    60181 tgactgttca cgctaatacg catactgtcc agcaccggcg aattaagaag caccagtatc
    60241 accaacacca ataacgaaac cgcaaggttc actttgccct gaagaacaag gggattttgc
    60301 ccattacgcc agacgatact gacaaaatac cccagcgacc acaccagtaa caccagctcc
    60361 gccagcgcgc cctgcaagcg gtcgacagtc cagccgtact gagcgacccg cagccataac
    60421 ccccaggtgg cgacgaacac atacagcgga gcaaccagca aagcggtttt aatcaggcaa
    60481 cgcaacggcc ccgtccaggg aagtgacgct ttttgcggat cgcggacaat agccattaag
    60541 atcaattgca aaaaggccag cgttaacagc aacccggcgg cggagatgtg gcgagaaatc
    60601 gcgctcaggc ccgtaaacgg cagggtgatg ataaacatca gggttagcaa tgatactaac
    60661 ggcagcaacc ccgtggcgat tagcgtgaac aacttttgaa tagagtcgat taaacgtgac
    60721 tgtgtacgcg cgaggatcac cgccagtgcg gtaaccagac caaatgtaag atacataaac
    60781 cagtcggttg caaaaaagag cgtattgaaa aacgtgatgc caacaagttt aaacaactca
    60841 ccccacagta aaagcaccag ccacgtcagg ccattggaga gaaaaatcac cagtaatata
    60901 agtacgttat gccatactga ctggtaaaag tagctatagc ggctactgcc attgcggata
    60961 cgaaggcttt gttgtatcca cggtagcaac aacattgcca tcaacagcag atagcagcca
    61021 aaatcccaaa tcgccttttc agctctccag gggttcatgc catcagtttg ccacttcagc
    61081 caaccgctca tccctgtcgt ggcaataaac accagcgcca gccatcccca aaggcgcttc
    61141 tgtttaaaag agatcacgga gaagagtaaa acggatgaaa acgcgacggt cgcaggcacg
    61201 ccataaacaa tccaactgtg attttttccg gcaagccagg cgataagcag gtagcaaacc
    61261 agcccctgaa gtaatccggt agcaatcatc ccctgaagta atccggtagc aatcatcccc
    61321 cagcgtgttg ccggtgaaag ttcaacgtta tccatgttgg ttatccagaa tcaaaaggtg
    61381 ggttaattat cgcatccggg cagtagtatt ttgctttttt cagaaaataa tcaaaaaaag
    61441 ttagcgtggt gaatcgatac tttaccggtt gaatttgcat caatttcatt caggaatgcg
    61501 attccactca caatattccc gccatataaa ccaagattta accttttgag aacattttcc
    61561 acacctaaaa tgctatttct gcgataatag caaccgtttc gtgacaggaa tcacggagtt
    61621 ttttgtcaaa tatgaatttc tccagatacg taaatctatg agccttgtcg cggttaacac
    61681 ccccaaaaag actttactat tcaggcaata catattggct aaggagcagt gaaatgcgcg
    61741 ttaacaatgg tttgcccccg caagaactcg aggcttatgg tatcagtgac gtacatgata
    61801 tcgtttacaa cccaagctac gacctgctgt atcaggaaga gctcgatccg agcctgacag
    61861 gttatgagcg cggggtgtta actaatctgg gtgccgttgc cgtcgatacc gggatcttca
    61921 ccggtcgttc accaaaagat aagtatatcg tccgtgacga taccactcgc gatactttct
    61981 ggtgggcaga caaaggcaaa ggtaagaacg acaacaaacc tctctctccg gaaacctggc
    62041 agcatctgaa aggcctggtg accaggcagc tttccggcaa acgtctgttc gttgtcgacg
    62101 ctttctgtgg tgcgaacccg gatactcgtc tttccgtccg tttcatcacc gaagtggcct
    62161 ggcaggcgca ttttgtcaaa aacatgttta ttcgcccgag cgatgaagag ctggcaggtt
    62221 tcaaaccaga ctttatcgtt atgaacggcg cgaagtgcac taacccgcag tggaaagaac
    62281 agggtctgaa ctccgaaaac ttcgtggcgt ttaacctgac cgagcgcatg cagctgattg
    62341 gcggcacctg gtacggcggc gaaatgaaga aagggatgtt ctcgatgatg aactacctgc
    62401 tgccgctgaa aggtatcgct tctatgcact gctccgccaa cgttggcgag aaaggcgatg
    62461 ttgcggtgtt cttcggcctt tccggcaccg gtaaaaccac cctttccacc gacccgaaac
    62521 gccgcctgat tggcgatgac gaacacggct gggacgatga cggcgtgttt aatttcgaag
    62581 gcggctgcta cgcaaaaact atcaagctgt cgaaagaagc ggaacctgaa atctacaacg
    62641 ctatccgtcg tgatgcgctg ctggaaaacg tcaccgtgcg tgaagatggc actatcgact
    62701 ttgatgatgg ttcaaaaacc gagaacaccc gcgtttctta tccaatctat cacatcgata
    62761 acatcgttaa gccggtttcc aaagcgggcc acgcgactaa ggttatcttc ctgactgctg
    62821 atgctttcgg cgtgttgccg ccggtttctc gcctgactgc cgatcaaacc cagtatcact
    62881 tcctctctgg cttcaccgcc aaactggccg gtactgagcg tggcatcacc gaaccgacgc
    62941 caaccttctc cgcttgcttc ggcgcggcgt tcctgtcgct gcacccgact cagtacgcag
    63001 aagtgctggt gaaacgtatg caggcggcgg gcgcgcaggc ttatctggtt aacactggct
    63061 ggaacggcac tggcaaacgt atctcgatta aagatacccg cgccattatc gacgccatcc
    63121 tcaacggttc gctggataat gcagaaacct tcactctgcc gatgtttaac ctggcgatcc
    63181 caaccgaact gccgggcgta gacacgaaga ttctcgatcc gcgtaacacc tacgcttctc
    63241 cggaacagtg gcaggaaaaa gccgaaaccc tggcgaaact gtttatcgac aacttcgata
    63301 aatacaccga cacccctgcg ggtgccgcgc tggtagcggc tggtccgaaa ctgtaatgat
    63361 ttgaagctgg agaatatcta tccagtatct tatagaaagc aaaacgggag gcaccttcgc
    63421 ctcccgttta tttacccttc ttttgtcgtg ccctgcgccc gcgttaccgg cactggcagc
    63481 caggcgcgaa tggaaagccc gccccgctcg ctggtgccaa gctccagcat cccgttatgg
    63541 ttatccacga tacgctgcac aattgccagc cctaatcccg tgccgctaat ggtgcgcgca
    63601 ctgtcgccgc ggacaaacgg ctggaacagg tgcttacgtt gttccggcgc aattcccgga
    63661 ccgtcatctt ccacctggaa ccaggcgcga ttcggctccg ttccgctgct gactttgatc
    63721 cagccattgc cataacgggc ggcgttgacc accatattcg ccaccgcgcg tttgatcgac
    63781 agcgggtgca ttttcacttc aatgctgccg gggtaaagcg cggtttcaat ttcccgctca
    63841 tagccacttt cggcagcaat cacctcaccg agcactgcat taagatccgc catttccatc
    63901 ggcatctcct gcccggtgcg caggtagtcg ataaactgct caatgatggc gttgcactct
    63961 tcgatatctt tattgatcga ttctgccaga tagccatcct gctcgctcat catctcagtc
    64021 gccaggcgaa tacgcgtcag cggcgtgcgc aagtcgtgac ttacccccgc catcagcagc
    64081 gtgcggtcat ccgccagttg cttaacacca gccgccatat ggttaaaggc acgggtaacg
    64141 gaacgcacct ccgaagcgcc atattcacgc agcggcggcg gaataatccc tttaccaacc
    64201 tgcaaggctg cgtgttcgag atcgaccaac ggtcggttct ggatacgaat aaacagccac
    64261 gccccgccta tcgccaatag cataatcgcc agcgtatagc ggaacagcgg agagaaatcg
    64321 ccctgatgaa tttcggtcag cggcacgcgt acccagatat tgggcgacag ccaggttttc
    64381 agccagacga caggcgaact tttgttgacc tcaacgcgca cttccgtcgg gccgcccagt
    64441 tgctgtgcca tctgatggct taagaattca tagtgttgcg cccaacgcag acctgcctct
    64501 tcggcagcct cgttggagta gagagagatc cccagctcac ggtagatctc ccgacggaaa
    64561 gcgggaggca caaccaactg cgtgccgtcc tccagttgca gtttgtcggt catcaacata
    64621 cgcacttcgt acgcgaggac tttattaaac tgctggaggc tcggcaaaat cgcgaagttc
    64681 agcaccacca gataagtcgt caccaggctg gcgaacagca aggtgacgat gagcaataac
    64741 gtacgggcaa atgaacttcg tggcgagaag cgcaatcgcc tcatgcttta gagccgtccg
    64801 gtacaaagac gtagcccagg ccccaaacgg tctgaatgta acgcggatgc gctggatctt
    64861 cttctaccat gcggcgcaga cgcgaaatct gcacgtcgat ggagcgttcc attgcggaat
    64921 attcacgacc acgggcaagg ttcatcagct tatcgcggga gagcggctca cgcggatggc
    64981 tgaccagtgc cttcagtacc gcaaactcac cgctggtgag cggcatcggc tcatcttcgc
    65041 ggaacatttc gcgcgtaccg aggttaagtt tgaacttacc gaaagcaatt accgcctctt
    65101 cctgtgacgg tgcgcctggc agttcgttcg cctgacgacg cagcaccgca cgaatacggg
    65161 ccagcagttc acgcgggtta aacggttttg gaatgtagtc gtcagcgcca atctccaggc
    65221 ctacgatacg gtccacttct tcccctttcg ccgtcaccat aatgatcggc atcgggttgc
    65281 tctgactacg aagacgtcgg caaatcgaca agccatcttc accaggtaac attaaatcca
    65341 gtaccataag atggaaagat tcacgagtca gcaggcgatc catctgttct gcattagcga
    65401 cgcttcgaac ctggaagcct tgttcggtga gataacgttc cagcagcgca cgcaggcgca
    65461 tgtcgtcatc gaccaccaga atcttgtagt tctcttgcat tgtttgtact cccaaaggtt
    65521 cgcaacaatt tgtaagcgtg tattcttaaa aaagctcacg ttcgtcacca gctaaatctg
    65581 gtatgaattt cagcctaaat tgttacaaag catattaaac agcagcttaa gtatacaatt
    65641 tattcggcga aacattattg attctgttga tatgatcacg ttatacccaa tgtgcgcatt
    65701 atcaaacaga caaagggaat caacgagatg aaaacgcccc tggttacccg ggaagggtat
    65761 gaaaaactca aacaagagct taattatctc tggcgtgaag aacgcccgga ggtcacaaaa
    65821 aaggtgacct gggccgcaag tctgggcgac cgcagcgaaa atgctgacta tcagtataat
    65881 aaaaagcgtc tgcgtgaaat cgaccgtcgc gtgcgctatc tcactaaatg cctggaaaat
    65941 ctcaaaatcg tcgattactc ccctcagcag gaaggcaaag tcttttttgg cgcgtgggtg
    66001 gagattgaaa acgacgatgg cgtgactcac cgtttccgta ttgtcggcta cgatgaaatt
    66061 tttggccgta aagattacat ctctatcgat tccccgatgg cccgcgcatt gctgaaaaaa
    66121 gaagtcggcg atctggcggt ggtgaatacc cctgccgggg aagcgaactg gtatgttaat
    66181 gctatcgagt acgtgaaacc gtaaggaaga gtcttaacct cctgccgatg gctggcattt
    66241 ttgccagcca gtccgtataa ctatcccctg atttttgatc cgaaaagatg aactcaaacc
    66301 atgatgaatg attcgttctg ccgcattatt gcgggtgaaa ttcaggcgcg cccggaacag
    66361 gttgacgctg ccgttcgcct gcttgacgaa gggaataccg tgccgtttat cgcacgttat
    66421 cgtaaggaaa tcaccggcgg tctggatgac acgcagctgc gtaatctgga aacgcgtctg
    66481 agctatctgc gcgagctgga agagagacgt caggcgatcc tcaaatccat ttccgagcaa
    66541 ggcaaactca ccgatgatct ggcgaaggcc atcaacgcca ccctaagcaa aaccgaactc
    66601 gaagacctct acctgcccta caaacctaaa cgccgcaccc gcgggcaaat cgccattgaa
    66661 gcagggcttg agccgttggc tgacctgctg tggagcgatc cgtcacacac gccagaagtc
    66721 gccgctgcac aatatgttga tgccgataaa ggcgtggcag ataccaaagc cgcgctggat
    66781 ggcgcgcgct atatcctgat ggaacggttt gccgaagatg ccgcgctgct ggcgaaagtg
    66841 cgtgattatc tgtggaagaa cgcgcatttg gtttctacgg tggtgagcgg taaagaagag
    66901 gaaggggcga aattccgcga ctatttcgat catcacgaac cgttgtccac ggtgccttct
    66961 caccgcgcgc tggcgatgtt ccgtgggcgt aacgaaggcg tactccagct ttcgctgaat
    67021 gccgatccgc agttcgatga gccgcccaaa gagagctatt gcgagcaaat catcatggat
    67081 caccttggcc tgcgcctgaa caatgccccg gcggatagct ggcgcaaaag cgtagtgagc
    67141 tggacctggc gcatcaaggt gctgatgcat ctggaaaccg aactgatggg taccgtgcgc
    67201 gaacgcgcgg aagatgaagc aatcaacgtc tttgcccgta acctgcacga tctgctgatg
    67261 gcggcccctg ccggactgcg tgcaacgatg ggcctcgatc cgggtctgcg taccggggta
    67321 aaagtggcgg tggtcgatgc cactggcaaa ctggtggcga ccgataccat ttacccgcac
    67381 accggacaag ccgcaaaagc agcgatgacc gttgctgcct tgtgtgaaaa acataacgtt
    67441 gaactggtgg cgatcggcaa cggtacagct tcccgcgaaa ctgaacgttt ctatctcgac
    67501 gtgcagaagc agttcccgaa agtgaccgca cagaaagtga tcgtcagcga agctggcgcg
    67561 tcggtttact cagcttccga gctggcagcg caggagttcc cggatctcga cgtttcgctg
    67621 cgtggtgcgg tatctatcgc ccgccgtttg caggatccgc tggcggagct ggtgaaaatc
    67681 gatccgaaat ctatcggcgt aggtcagtat cagcatgacg tcagccagac gcaactggcc
    67741 cgcaaactgg atgcagtagt agaagactgc gtaaacgccg ttggcgtcga tctcaacacc
    67801 gcttctgttc cgctattaac ccgcgtggcg ggcctgacgc gcatgatggc gcaaaacatc
    67861 gtggcctggc gcgatgagaa cggccagttc cagaaccgtc agcaactgct gaaagtcagc
    67921 cgtctggggc cgaaagcctt cgagcagtgc gcgggcttcc tgcgcattaa ccacggtgat
    67981 aacccgctgg acgcctctac cgttcatccg gaagcctatc cggtggtgga acgcattctg
    68041 gcagcaacac agcaggcact gaaagatctg atgggtaaca gcagcgaact gcgtaacctg
    68101 aaagcgtctg actttactga tgaaaaattc ggtgtgccga cagtaactga catcatcaaa
    68161 gagctggaaa aaccgggtcg cgatccgcgt ccggaattta aaaccgctca gtttgccgat
    68221 ggcgtcgaga caatgaacga cctgcaaccg ggtatgatcc tcgaaggcgc agtgaccaac
    68281 gtcaccaact ttggcgcgtt tgtcgatatt ggcgtgcatc aggacggcct ggttcacatc
    68341 tcttcattgt cgaacaagtt tgtggaagat ccgcataccg tggtgaaagc gggcgacatt
    68401 gtgaaggtga aagtgctgga agtggatctt cagcgtaaac gtatcgccct gactatgcgt
    68461 ctggacgagc agcctggcga aaccaactct cgtcgcggcg gcggtaatga acgcccgcaa
    68521 aacaaccgcc cggcagccaa accacgcggt cgtgaagcgc agcctgccgg taatagcgcg
    68581 atgatggatg cgctggcggc agcgatgggt aaaaaacgtt aaacgcccgg actggcccct
    68641 aaacctgcgc gataaggcgt tcacgccgca tctggcaacg aacgctttgc ctgacataaa
    68701 agtgccggag aatatctccg gcacttttat tccacagcca aactcacaat atactccgtt
    68761 aatatttatc acttcattaa caactgaaac cttaattaaa cattagccag tccgggtaac
    68821 tcactattcg aattatattt tcgctgcgat ataaccttga gccacatcaa cattgagtca
    68881 gattattatt caaaccaaca ttcgcacaca ttttaagtat tgctgataga aaccattctc
    68941 attatcattg tgttgttgat tatttaatct ctcctttgtt ggcaaatcat ctggtctcat
    69001 gtcgcggtca aacgccccat gaggtagtta tccagttaat gagaaacaag taggcaccta
    69061 tgcaatacac tccagatact gcgtggaaaa tcactggctt ttcccgtgaa atcagcccgg
    69121 catatcgcca aaaactgctt tctctaggca tgttacctgg ctcctctttt gatgtggtgc
    69181 gcgtcgctcc actcggcgac cccattcata tcgaaacccg tcgtgtgagc ctggtattac
    69241 gcaaaaaaga tctggcctta ttagaagtgg aagcggtttc ctgttaatac ggtgataaca
    69301 acaatgaaaa aattaaccat tggcttaatt ggtaatccaa attctggcaa gacaacctta
    69361 tttaaccagc tcactggcgc acgtcagcgt gtaggtaact gggctggcgt taccgtcgaa
    69421 cgtaaagaag ggcaattctc caccaccgat catcaggtca cgctggtgga cctgcccggc
    69481 acctattctc tgaccaccat ctcatcgcag acctcgctcg atgagcaaat cgcctgtcac
    69541 tacattttga gtggcgacgc cgacctgctg attaacgtgg tggatgcgtc taaccttgag
    69601 cgtaacctgt acctgacgct gcaactgctg gaactcggca ttccctgcat cgtggcgctg
    69661 aacatgctcg acattgccga gaagcaaaat attcgtattg aaattgatgc tctatcggca
    69721 cgtcttggct gcccggtgat cccgctggtt tcaacccgtg gtcgcggtat tgaagcgctc
    69781 aagctggcga ttgatcgcta taaagctaac gagaatgtgg aactggtgca ttacgcacag
    69841 ccgctactca acgaagcaga ttcactggca aaagtgatgc cttccgacat cccgctgaaa
    69901 caacgtcgct ggctgggcct gcaaatgctg gaaggcgata tctacagccg cgcctacgcc
    69961 ggtgaagcgt cgcagcatct ggatgccacc ctcgcccgtc tgcgtaatga aatggatgat
    70021 ccggcgctgc acattgccga tgcgcgttac cagtgcattg ctgccatctg tgatgtggta
    70081 agcaacaccc tgacggcaga acccagccgt ttcaccactg cggtagataa aatcgtgctc
    70141 aaccgtttcc tcggtctgcc gattttcctc tttgtgatgt acctgatgtt cctgctggct
    70201 atcaacatcg gcggggcgtt acagccgctg tttaacgtcg gctccgtggc gctatttgtg
    70261 catggtattc aatggattgg ctacacgctc cacttcccgg actggctgac tatcttcctc
    70321 gcccagggcc tgggtggcgg tattaacacc gtgctgccac tggtgccgca gatcggcatg
    70381 atgtacctgt tcctctcctt ccttgaggac tccggctata tggcgcgtgc agcgtttgtg
    70441 atggaccgcc tgatgcaggc gctgggcttg ccggggaaat cctttgtgcc gctgatcgtc
    70501 ggtttcggtt gtaacgtacc gtcggtaatg ggcgcacgta cgcttgatgc accgcgtgaa
    70561 cgtctgatga ccatcatgat ggcaccgttt atgtcctgcg gcgcgcgtct ggctatcttc
    70621 gcagtattcg cggctgcctt cttcgggcag aacggtgccc tggcggtttt ctcgctgtat
    70681 atgctcggta tcgtgatggc ggtgctgact ggcctgatgc tcaagtacac catcatgcgc
    70741 ggtgaagcga cgccgtttgt catggagctg ccggtctacc atgtaccaca cgttaaaagc
    70801 ctgattatcc agacctggca gcgtctgaag ggcttcgttc tgcgtgctgg taaagtgatt
    70861 atcatcgtca gcattttcct gagcgctttc aacagcttct cgttgagcgg gaaaatcgtc
    70921 gataacatca acgactcggc gctggcttcc gtcagccggg tgataacccc tgtcttcaag
    70981 ccgattggcg tacatgaaga taactggcag gcaacggttg gcctgtttac gggcgcaatg
    71041 gcaaaagaag tggtggtggg tacgctcaac accctctaca ccgcagaaaa tattcaggac
    71101 gaagagttca atccggcgga attcaacctc ggtgaagagc tgttcagtgc ggtagatgaa
    71161 acctggcaga gcctgaaaga caccttcagc cttagcgtac tgatgaaccc cattgaagct
    71221 agcaaaggca acggtgaaat gggtaccggg gcgatgggcg tgatggatca gaaattcggt
    71281 agcgcagctg ccgcatacag ctacctgatt ttcgtcctgc tgtatgtacc atgtatctcg
    71341 gtgatgggag ccatcgcccg tgaatcaagc cgtggctgga tgggcttctc catcctgtgg
    71401 gggctgaata tcgcttactc actggcaaca ttgttctatc aggtcgccag ctacagtcag
    71461 cacccaactt acagcctggt gtgcattctg gcggtaatcc tgtttaacat cgtagctatc
    71521 ggtctgctgc gccgcgcgcg tagccgggtg gatatcgaac tgctggcaac ccgcaagtcg
    71581 gtaagcagtt gctgcgcagc cagcaccacc ggtgattgcc attaatggct tcacttattc
    71641 aggtacgtga tttgctggcg ttacggggcc gtatggaagc gacccagata agccagacat
    71701 tgaacactcc gcagccaatg attaacgcca tgctgcaaca gctggaaagc atgggcaaag
    71761 ccgtgcggat tcaggaagaa cctgacggct gcctctctgg cagttgtaaa agctgcccgg
    71821 aaggcaaagc ctgtctgcgc gagtggtggg cgctgcgtta accatactcc atcgcctgtt
    71881 ttgaaaagcc cggtatgcgt ttgcatccgg gcttttttgc gtgcggcttt ccataaaaat
    71941 gcaattcttg cagcgcaacg taagttcctc tgaaagcgtc tcgcagggat gaaaactcgc
    72001 taatgcacag atgtggagtg gcgcgtagag tcgcggcatt caaacaacag gtgaaggaac
    72061 gccatgagta aaaagcagag ttccacccca catgatgcgc tgttcaaact ctttttacgc
    72121 caaccggaga cggcacgcga ttttcttgcg tttcatttac cggcacccat tcacgcgctc
    72181 tgtgatatga aaaccctcaa gctggagtcg aggagcttta ttgatgacga tctgcgtgaa
    72241 agctattccg atgtgctgtg gtcggtgaaa acggaacaag gaccaggata catctattgt
    72301 ctgattgaac atcaaagcac ctcaaacaaa ctgatcgcat ttcgcatgat gcgttacgct
    72361 attgccgcaa tgcaaaatca ccttgatgct ggatacaaaa cgttgccgat ggtggtgcca
    72421 ttgttgtttt accacggtat tgaaagcccc tatccctatt cgctgtgttg gctggattgt
    72481 ttcgccgatc ccaaactggc aaggcagctt tatgcctccg catttccgct gattgatgtc
    72541 accgtcatgc ctgatgatga aatcatgcag caccgacgta tggcgctgct ggagttaatt
    72601 caaaaacata ttcgtcaacg cgatctgatg gggctggtag agcaaatggc ctgcttatta
    72661 agtagtggat acgctaatga cagacaaatc aaagggctgt ttaattacat actgcaaact
    72721 ggcgacgctg tacgttttaa cgattttatc gacggcgttg ccgaacgttc accgaaacac
    72781 aaggagagtt taatgactat tgcggaaaga ttgcggcagg agggggaaca atccaaagcc
    72841 ctgcatatag ccaaaataat gcttgaatcc ggagtccctc ttgcagacat catgcgattt
    72901 accgggctgt cagaagaaga gttggctgcg gcgagtagac tggccccctg aatctccaga
    72961 caaccag
//
LOCUS       DAJTRD010000005        72097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTRD010000005 DAJTRD010000000
VERSION     DAJTRD010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.005"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(1371..2957)
                     /locus_tag="OO315_000388"
     CDS             complement(1371..2957)
                     /locus_tag="OO315_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5889428.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(3312..5483)
                     /gene="glcB"
                     /locus_tag="OO315_000389"
     CDS             complement(3312..5483)
                     /gene="glcB"
                     /locus_tag="OO315_000389"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5889429.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5505..5909)
                     /locus_tag="OO315_000390"
     CDS             complement(5505..5909)
                     /locus_tag="OO315_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5889430.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5914..7133)
                     /gene="glcF"
                     /locus_tag="OO315_000391"
                     /pseudo
     CDS             complement(5914..7133)
                     /gene="glcF"
                     /locus_tag="OO315_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(7144..8196)
                     /gene="glcE"
                     /locus_tag="OO315_000392"
     CDS             complement(7144..8196)
                     /gene="glcE"
                     /locus_tag="OO315_000392"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5889431.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(8196..9695)
                     /gene="glcD"
                     /locus_tag="OO315_000393"
                     /pseudo
     CDS             complement(8196..9695)
                     /gene="glcD"
                     /locus_tag="OO315_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9946..10716
                     /gene="glcC"
                     /locus_tag="OO315_000394"
     CDS             9946..10716
                     /gene="glcC"
                     /locus_tag="OO315_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5889432.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10840..11981)
                     /locus_tag="OO315_000395"
                     /pseudo
     CDS             complement(10840..11981)
                     /locus_tag="OO315_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12343..14073
                     /locus_tag="OO315_000396"
     CDS             12343..14073
                     /locus_tag="OO315_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5889433.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            14070..14984
                     /locus_tag="OO315_000397"
     CDS             14070..14984
                     /locus_tag="OO315_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5889434.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            15017..15265
                     /locus_tag="OO315_000398"
     CDS             15017..15265
                     /locus_tag="OO315_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5889435.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15265..16436
                     /locus_tag="OO315_000399"
                     /pseudo
     CDS             15265..16436
                     /locus_tag="OO315_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16472..17551)
                     /gene="lptG"
                     /locus_tag="OO315_000400"
     CDS             complement(16472..17551)
                     /gene="lptG"
                     /locus_tag="OO315_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5889436.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17548..18618)
                     /gene="lptF"
                     /locus_tag="OO315_000401"
     CDS             complement(17548..18618)
                     /gene="lptF"
                     /locus_tag="OO315_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5889437.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18649..19209)
                     /locus_tag="OO315_000402"
     CDS             complement(18649..19209)
                     /locus_tag="OO315_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5889438.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(19221..20045)
                     /locus_tag="OO315_000403"
     CDS             complement(19221..20045)
                     /locus_tag="OO315_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889439.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(20045..21394)
                     /locus_tag="OO315_000404"
     CDS             complement(20045..21394)
                     /locus_tag="OO315_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5889440.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21440..22198)
                     /locus_tag="OO315_000405"
     CDS             complement(21440..22198)
                     /locus_tag="OO315_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889441.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(22230..22943)
                     /locus_tag="OO315_000406"
     CDS             complement(22230..22943)
                     /locus_tag="OO315_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5889442.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            23117..23809
                     /locus_tag="OO315_000407"
     CDS             23117..23809
                     /locus_tag="OO315_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5889443.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23858..25357)
                     /gene="pitB"
                     /locus_tag="OO315_000408"
     CDS             complement(23858..25357)
                     /gene="pitB"
                     /locus_tag="OO315_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5889444.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25650..27509)
                     /gene="gss"
                     /locus_tag="OO315_000409"
     CDS             complement(25650..27509)
                     /gene="gss"
                     /locus_tag="OO315_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5889445.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27714..28580
                     /gene="yghU"
                     /locus_tag="OO315_000410"
     CDS             27714..28580
                     /gene="yghU"
                     /locus_tag="OO315_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5889446.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28703..28951)
                     /gene="hybG"
                     /locus_tag="OO315_000411"
     CDS             complement(28703..28951)
                     /gene="hybG"
                     /locus_tag="OO315_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5889447.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(29085..29426)
                     /gene="hypA"
                     /locus_tag="OO315_000412"
     CDS             complement(29085..29426)
                     /gene="hypA"
                     /locus_tag="OO315_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5889448.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29419..29907)
                     /gene="hybE"
                     /locus_tag="OO315_000413"
     CDS             complement(29419..29907)
                     /gene="hybE"
                     /locus_tag="OO315_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5889449.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29900..30394)
                     /locus_tag="OO315_000414"
     CDS             complement(29900..30394)
                     /locus_tag="OO315_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5889450.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30394..32097)
                     /gene="hybC"
                     /locus_tag="OO315_000415"
     CDS             complement(30394..32097)
                     /gene="hybC"
                     /locus_tag="OO315_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5889451.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(32094..33272)
                     /gene="hybB"
                     /locus_tag="OO315_000416"
     CDS             complement(32094..33272)
                     /gene="hybB"
                     /locus_tag="OO315_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5889452.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33262..34248)
                     /gene="hybA"
                     /locus_tag="OO315_000417"
     CDS             complement(33262..34248)
                     /gene="hybA"
                     /locus_tag="OO315_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5889453.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34251..35369)
                     /gene="hybO"
                     /locus_tag="OO315_000418"
     CDS             complement(34251..35369)
                     /gene="hybO"
                     /locus_tag="OO315_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5889454.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35558..35845)
                     /locus_tag="OO315_000419"
     CDS             complement(35558..35845)
                     /locus_tag="OO315_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5889455.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35964..36851)
                     /locus_tag="OO315_000420"
     CDS             complement(35964..36851)
                     /locus_tag="OO315_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5889456.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            37008..38048
                     /gene="gpr"
                     /locus_tag="OO315_000421"
     CDS             37008..38048
                     /gene="gpr"
                     /locus_tag="OO315_000421"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5889457.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(38088..38582)
                     /locus_tag="OO315_000422"
     CDS             complement(38088..38582)
                     /locus_tag="OO315_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5889458.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38773..39657
                     /gene="yghA"
                     /locus_tag="OO315_000423"
     CDS             38773..39657
                     /gene="yghA"
                     /locus_tag="OO315_000423"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5889459.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPSIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39929..40354)
                     /gene="exbD"
                     /locus_tag="OO315_000424"
     CDS             complement(39929..40354)
                     /gene="exbD"
                     /locus_tag="OO315_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5889460.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40361..41095)
                     /gene="exbB"
                     /locus_tag="OO315_000425"
     CDS             complement(40361..41095)
                     /gene="exbB"
                     /locus_tag="OO315_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5889461.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41347..42534
                     /gene="metC"
                     /locus_tag="OO315_000426"
     CDS             41347..42534
                     /gene="metC"
                     /locus_tag="OO315_000426"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5889462.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42674..43333
                     /locus_tag="OO315_000427"
     CDS             42674..43333
                     /locus_tag="OO315_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5889463.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43373..44272)
                     /gene="yqhC"
                     /locus_tag="OO315_000428"
     CDS             complement(43373..44272)
                     /gene="yqhC"
                     /locus_tag="OO315_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5889464.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44466..45629
                     /gene="yqhD"
                     /locus_tag="OO315_000429"
     CDS             44466..45629
                     /gene="yqhD"
                     /locus_tag="OO315_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5889465.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45734..46561
                     /gene="dkgA"
                     /locus_tag="OO315_000430"
     CDS             45734..46561
                     /gene="dkgA"
                     /locus_tag="OO315_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5889466.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46761..47687
                     /locus_tag="OO315_000431"
     CDS             46761..47687
                     /locus_tag="OO315_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5889467.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47738..47995
                     /gene="yqhH"
                     /locus_tag="OO315_000432"
     CDS             47738..47995
                     /gene="yqhH"
                     /locus_tag="OO315_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5889468.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(48038..50257)
                     /locus_tag="OO315_000433"
     CDS             complement(48038..50257)
                     /locus_tag="OO315_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5889469.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50368..51780)
                     /gene="ftsP"
                     /locus_tag="OO315_000434"
     CDS             complement(50368..51780)
                     /gene="ftsP"
                     /locus_tag="OO315_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5889470.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51855..52592)
                     /gene="plsC"
                     /locus_tag="OO315_000435"
                     /gene_synonym="parF"
     CDS             complement(51855..52592)
                     /gene="plsC"
                     /locus_tag="OO315_000435"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5889471.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52826..55084)
                     /gene="parC"
                     /locus_tag="OO315_000436"
     CDS             complement(52826..55084)
                     /gene="parC"
                     /locus_tag="OO315_000436"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5889472.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55630..56112)
                     /locus_tag="OO315_000437"
     CDS             complement(55630..56112)
                     /locus_tag="OO315_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5889473.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSELIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(56165..56557)
                     /gene="ygiW"
                     /locus_tag="OO315_000438"
     CDS             complement(56165..56557)
                     /gene="ygiW"
                     /locus_tag="OO315_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5889474.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56709..57368
                     /gene="qseB"
                     /locus_tag="OO315_000439"
     CDS             56709..57368
                     /gene="qseB"
                     /locus_tag="OO315_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5889475.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57365..58639
                     /gene="qseC"
                     /locus_tag="OO315_000440"
     CDS             57365..58639
                     /gene="qseC"
                     /locus_tag="OO315_000440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5889476.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58685..59017)
                     /locus_tag="OO315_000441"
     CDS             complement(58685..59017)
                     /locus_tag="OO315_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5889477.1"
                     /translation="MLKQKIKTIFEALLYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59336..59917
                     /gene="mdaB"
                     /locus_tag="OO315_000442"
     CDS             59336..59917
                     /gene="mdaB"
                     /locus_tag="OO315_000442"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5889478.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59948..60262
                     /locus_tag="OO315_000443"
     CDS             59948..60262
                     /locus_tag="OO315_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5889479.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60310..62202)
                     /gene="parE"
                     /locus_tag="OO315_000444"
     CDS             complement(60310..62202)
                     /gene="parE"
                     /locus_tag="OO315_000444"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5889480.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62231..62812)
                     /gene="yqiA"
                     /locus_tag="OO315_000445"
     CDS             complement(62231..62812)
                     /gene="yqiA"
                     /locus_tag="OO315_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5889481.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62812..63639)
                     /gene="cpdA"
                     /locus_tag="OO315_000446"
     CDS             complement(62812..63639)
                     /gene="cpdA"
                     /locus_tag="OO315_000446"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5889482.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63664..64086)
                     /locus_tag="OO315_000447"
     CDS             complement(63664..64086)
                     /locus_tag="OO315_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5889483.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(64087..64716)
                     /gene="nudF"
                     /locus_tag="OO315_000448"
     CDS             complement(64087..64716)
                     /gene="nudF"
                     /locus_tag="OO315_000448"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5889484.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64921..66402
                     /gene="tolC"
                     /locus_tag="OO315_000449"
     CDS             64921..66402
                     /gene="tolC"
                     /locus_tag="OO315_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5889485.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66550..67221
                     /locus_tag="OO315_000450"
     CDS             66550..67221
                     /locus_tag="OO315_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5889486.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67227..68387
                     /locus_tag="OO315_000451"
     CDS             67227..68387
                     /locus_tag="OO315_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5889487.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68425..69213)
                     /gene="ygiD"
                     /locus_tag="OO315_000452"
     CDS             complement(68425..69213)
                     /gene="ygiD"
                     /locus_tag="OO315_000452"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5889488.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     LNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69356..70129
                     /gene="zupT"
                     /locus_tag="OO315_000453"
     CDS             69356..70129
                     /gene="zupT"
                     /locus_tag="OO315_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5889489.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(70187..70357)
                     /locus_tag="OO315_000454"
     CDS             complement(70187..70357)
                     /locus_tag="OO315_000454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889490.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70618..71271)
                     /gene="ribB"
                     /locus_tag="OO315_000455"
     CDS             complement(70618..71271)
                     /gene="ribB"
                     /locus_tag="OO315_000455"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5889491.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71375..71523)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71645..71935
                     /gene="ubiK"
                     /locus_tag="OO315_000456"
     CDS             71645..71935
                     /gene="ubiK"
                     /locus_tag="OO315_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5889492.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        18039 a        19162 c        17738 g        17158 t
ORIGIN      
        1 ttacgtaccg tttaaacaaa gacaatacgt ctctttattt ctcataaaac cccaatccct
       61 tacagggctt attgaaaaat ttaaaaatca caaatttctc aatactcgaa atgagatcac
      121 ataagaaatc aaaaagcctt ctcatctatt ttaagttttc ccaacggaaa ataagagctt
      181 tcattaatac tgaaccactt tactgttcta tttgttcagg tattgcatta cggtgtgctg
      241 agatccatcg gtgcatccct gtatcccgcg atgagactaa aatagtcgtg atgtaagctc
      301 gtatcagatt cttagtgaat accacatttg ttataattaa gtgaaaagat acattgctga
      361 tttacattat ttggtttaca aaatgtcatc acttgcagag ttatcgctga cagatacctt
      421 taatctctta ctactttcgt gttattgcaa tacaaccatc cgtaaagcag ggctattttt
      481 agcaggaaat actcaggtgt caacattcga actccaactc tgaaatattc atataagtta
      541 atgattttaa tgattttaat gattttaatg attttaatga ttttaatgat tttaatgatt
      601 ttaatgattt taatgatttt ttcatttatt cacacctaat aaaagtattc aaattcagat
      661 gatttttctg ctcaacagac acaaacaggc aataatactc tccaaagcga acaatatttg
      721 gcattacgat ttaatgcttc ataatcacca aattatttaa ccataaattt tcttatttct
      781 aacaaaatga ttggtgtgtt gttggagtta aaatacatac ataatattaa catctgccaa
      841 catttcactt tccgcaagac ggatgatttc gaatcctcat ccacgttacc ctatcccaaa
      901 tatctttatt aataggcagt aacgaacact gttatagcaa gattaactta ttaacctaac
      961 ggcatctttt atgtaacaag tggagataaa ttgcttaaaa ttgatcctcg acaaatctta
     1021 aaagttctca ttattctgca aaatcagctt tgcgaataat cttatgtttt tgggagtttt
     1081 gggaattttg atctactcat tgttctagtg aaaaatataa ataagagaaa aaacgccatt
     1141 cgtcatgaaa gcaacagatg aagcaatgta ataacatact aaaattgatc tcatgaaagt
     1201 gggtacttta taaccagcac agaagccaag ttgtataaat attaaacaat tcgcatcgta
     1261 aaacggagac tacgtataaa gaagccaatc gtaaaaacgg ttccctggcg agataaacga
     1321 cagacaaatg gaaagccggg tgatatttac cttcatccgg ctttattgat ttacgagact
     1381 aacatcccgg taaacacata cgcctgcagc agggtgataa cgctggcaaa aatcagactg
     1441 tgcttcacgg tgtagcggaa cagttcagat tctcggccca ccatgcccgt cgcggcgcag
     1501 gccacggcga tagattgcgg ggagatcatc ttgccagtta cgccgccgct ggtatttgct
     1561 gccaccagca gagtgtcaga gacgttgatt tgctgcgccg tggtcgattg cagtgaacca
     1621 aacagggcgt ttgaagaagt gtccgacccg gtaaggaata cgcccagcca gccgagaaac
     1681 ggtgagaaaa acgggaacat cacgcctgta cctgccagta ccagcgccag cgtggtggac
     1741 atgccggaat agttggtgac gaaggcgaat gccagcacca tgccaatcga caatatcggc
     1801 cacttcagac taattagcat ttcggcaaag acgccaatcc ctttcttgat gcccacgccg
     1861 aggatgaaga tagagataat cgccgcaata aaaatagcgg tgccgccagc cgagagaggg
     1921 tcgaatttaa acaccgcatc cattggcgtt ggttgggcga caatgggtgc cgctttcaac
     1981 acttgttgat gcaaatgagg gatctggaaa ttaatcacca gtgaataaaa cgcgccgccc
     2041 ggagcaaata acgctttaaa cggcttcatg gtccagatgg tgaccagcac cgttaagatt
     2101 aaaaacggtg accacgcacg aatgatttgc cccagactat attctgaagg cacgggaccg
     2161 ccagaagatg gcttatttac caccatcgca cctgcggatt gccccatgct gattgccgtt
     2221 tcggtatttt tcggccgcca gactttaagg aataaagcga gtgagacgat actcaccagc
     2281 gccgaagtaa tatccggcag ttccggacca atatagttag aggtaaagaa ctgagtgaca
     2341 gcgaagcttc ccccagcaac cagcgccgct ggccacgtct ctttcacccc tttccagccg
     2401 tccatcattg ctaccagcca gaacggcaca agaaccgaca ggaacggtaa ctgacgtccc
     2461 gccattgcgc caatgtggaa cggatcgatt cccgttacct gaccggcgac cagaatcggc
     2521 acgcccaacg caccaaacgc caccgtcgca ccaaagccag cagctccttc cagcaacgca
     2581 ccaaaggaga aaccaatcag taacacctgc aaacgctgat cgtcggtgat ggagataacc
     2641 gagctgcgga taatatcgaa ctgcccgctg gcaacggtta atttatacag gaacaccgcc
     2701 gcgacaataa tccacgcaat tggccataat ccataaataa agccatagcc cgcagcagca
     2761 aatgccatat caatcggcat tttaaaggcg aatatcgcaa tcaggataga taatataagg
     2821 gttattgctc cagcaacatg tcctttcaga cgtaataccg cgagtgcaac gaagaagaat
     2881 attatcggga tcagggcgac cagagcggat agccccagtc ctcccatcgg catatacatt
     2941 tgggtccagg taaccatatt gttcagtctc ttattatctt tatatgcttg atatacttaa
     3001 ggttgtaata agcaaaagag gactgaactg taaaatatag gcgttatact ttacagcaac
     3061 agtacgccgc taacgcaatt gctacctctg gcataacaag tatatcgggt aagggtttct
     3121 gttccgcaca cgcaggcgca gagtatcgtt aagatgtcca tattgttgtt ttaggcccgc
     3181 tagtaatgcg ctacgggtat ttaatattgt taaaccctga taatcgctcc ggttatttcc
     3241 gggataaatg tactaccgca gttactatca tagccccgac aataaaactt gccggggctt
     3301 ttttgacgct attaatgact ttctttttcg cgtaaacgcc aggcgtgtaa taacggttcg
     3361 gtataaccgt ttggctgttt cacgccgagg aagattaaat cgctggcagc tttaaaagca
     3421 cacgagttag cgaaattccc cgccatcgga cgataagccg gatcgccagc attttgctga
     3481 tcaaccactt tcgccatatt ctccagcgac gcctgcacct gttctttggt cagaataccg
     3541 tgacgtaacc agttggcgat atgctggctg gagatacgca gcgttgcgcg gtcttccatc
     3601 agcgccacgt tgtgaatatc cggcactttt gaacaaccaa tcccctgctc cacccaacgc
     3661 accacgtacc ccagaatccc ctgcacgttg ttatccagct cttgttggat ctcttgcgcc
     3721 gaccagttag cgttttcagc caccggaata gtcagcagat cgtccagcag cggttcaaat
     3781 tcagcactga actcggtctg ggcaatgttg gcttgcacgc tctgtacgtt ggtatggtgg
     3841 tagtgcagcg catggagcgt agcagcggtt ggtgacggaa cccaggctgt gtttgccccg
     3901 gcacgcagtt ggtcgccctt ctggctgtac atgtctgcca tcaggtccgg cattgcccac
     3961 atgcctttac cgatttgcgc tttaccgcgc agcccacaga acagaccgga aagcacgtta
     4021 ttacgttcgt aggctttgat ccacggcgtc gatttcatct ggtttttacg cagcatcggg
     4081 ccagcttcca tcaccgaatg catttcatcg ccggtacggt cgaggaaacc ggtattgatg
     4141 aacgccacgc ggttgcgcgc ctgagcgata cagctacgca agttcagcga ggtccggcgt
     4201 tcttcatcca taatgcccat tttcagggta ttcggtgcca taccgagcat tgtctcaatg
     4261 cgggtaaaca gtttgttggc gaacgccact tcctgcggac cgtgcatttt cggtttcaca
     4321 atatagacgc tgccagtgcg cgagtttttc tgcactttta aatcatagag ggcaatcgcg
     4381 ccagtcatga cgccatcaag aataccttcc gggatttcat tgccttcgct gtcccaaatc
     4441 acaggaatgg tcatcaaatg acccacgttg cggataaaca gcaacgagcg tccgtgcaga
     4501 gaaatttcag agccatcggc ggcggtgtac tgacgatcgt cattcagttt acgtacgatt
     4561 tgccgaccgt ttttctccat tttctcttgc agagtcccct gcatcaggcc cagcagattg
     4621 cggtacagca ggattttatc ttccgcatca accgccgcga ccgaatcttc gcagtcgaga
     4681 atggtactga tagcagcttc gacgataaca tcgttgatgt gcgccggatc gtctttgcca
     4741 atccgcccgt tggcatcaat ttgcagttca atatgcaggc cgttattttt cagcaaaatg
     4801 caggtcggcg cagcggcatc gccacggtaa ccgacaaact gtgctggagt acgtaacgtg
     4861 gtttctttac catttttcaa ctggatgcgt aattgtttat cgaccacctt aaacgccacc
     4921 acatcctgat agctgccgtt ttccagcggt agtgattcat cgaggaaacg tcgaacccag
     4981 gcgataacct gctcaccgcg ctgcggatcg tagccactga ccatcgcccc ttcctgcggg
     5041 atgatgtcgc tgccgtataa cgcatcgtac agtgagcccc agcgagcgtt cgccgcgttc
     5101 agcgcgtagc gggcgttcat tgccggaacc accagctgtg gccccgcctg actggtgatt
     5161 tcgctgtcaa tgcccgtggt ttccaccgtc acgcgctccg gttgcggcac caggtagccc
     5221 agttcacgaa ggaaagattt ataggccgct ttatctttta ccggccccgg attgctgcga
     5281 tgccactcat caagcgccgc ctgaatgcga tcgcgttctg ccagcaactg acgattttct
     5341 ggtgccagat cgtgaacgat ctcatcaaca ttgtgccaga atgcagcagc gtccagttcc
     5401 acgcccggca aaacttctcc atccacaaaa cgtttaaaat tggcgtcaat gcgtaaacgg
     5461 ccctgggtta tggtttgact cattgtttct ctcctcgttt tcgcttattt cgccaacacc
     5521 gctgctgcca ctttcgcgac ctgcgcatcc tgtgctccgg ttaaaccaga aacgcccacg
     5581 gcaccaataa tttgcccatc aacaacaacc ggtacgccgc cttccagcga cgtcagtaac
     5641 ggcgcagtca cgaacgcggt acgtccgttg ttcaccatct cttcatagcc cttagtttca
     5701 cgacgcccca gcgcggcggt acgcgctttc tcctgggaga tgtaagccgc aatcggcgcg
     5761 caatcgtcca tgcgacttaa cgccagcaga tgaccgccgt catcggcaac agcaatggaa
     5821 acagaccagt tatttttctg cgcctcttcc tgacctgcgg caataattgc actcgccatt
     5881 tgctggctaa gaatgacttt agttttcatt tttttattcc ttttcaaggg cttgttctac
     5941 aatttcaatc cagtgacgca cagaggtacg accggcgctc gccagatgcg tctggcaacc
     6001 aatgttggcg gtgacgatca tttccggttt gccgctttcc agcgcattca ttttgttatc
     6061 ccgcagctgg cgtgccagat cgggatgcgt taacgcatat gttcccgctg aaccgcagca
     6121 cagatggctg tcgggaacgt ccgttaaggt aaatccaaga cgaagtaaca ctttttccac
     6181 ttcgccgttc agcttttgcg catgttgtag ggtacacggg cagtggaagg ccagtttttt
     6241 atcgccgcga attgccagtt tttccagcgg ttcctcgcgc agaagttcga ctaaatcgac
     6301 cgccagttca ctgacctgac gcgctttatc ggcatataac gcatcgtttt tcagcatctg
     6361 cccatactct ttgacgaacg cgccgcaacc gctggcggtt tgcaaaattg cctcggcacc
     6421 tgcttcaatc gcgggccacc aggcatcaat attattgcgc gcccgtgcca accctttctc
     6481 ctgcgcatta agatgatagt ccaccgcgcc acaacagcct gcttcgttag ctgacatgac
     6541 gctgatcccc agacgatcca gcactcgcgc agttgccgcg ttggtgttgg gcgaaagcgt
     6601 aggctgggcg cagccttcca gcattaaaac ccgacgctta tggcgcagcg gcggacgcgg
     6661 tttagctttc accgtttcag caggcagttt tgctctgacc tgttccggta aaaacggtcg
     6721 cagcaccagc cctacctgcg tcagcgcacg gaagaccgcc ggacgcggca ctacctggcg
     6781 caatccttcg cgcagcattc gctccggcag tgggcgtttc actttctgct cgacaatatc
     6841 acgcccgata tccagcaaat tgtgatagcg cacaccagaa ggacaggtgg tttcacaatt
     6901 gcggcaagtg aggcagcgat cgagatgctc ctgtgtttta agcgtgactt cgttgccttc
     6961 cagcacctgt ttaatcagat agatgcgccc gcgcggcccg tccagttcat cgcccagaag
     7021 ctgataggtt gggcaggttg cggtacaaaa tccgcagtga acacaggcgc acaggatgct
     7081 gtcggcttcc agcgcgcgtt ctgccgcatc tcttcggtta atcgggtttg catagcctgc
     7141 tcctcaaagt tccgcgtaca tgcgaccggg gttaaacacg ccgcaagggt cgagctgctg
     7201 tttaagctgc tggtgatagc ggaataaagg agccgatagc ggggcaaagc caccatctcc
     7261 ggcactaaag cgggtcgcat gaccgccagc gttgcgggcg atgcgatgga tttgattgtc
     7321 atcggctgtc gatttcagcc agcgtaacgc cccgccccag tcgatcagtt gctcgccggg
     7381 taaatccatc atcggcgcat cgctgggtaa tgaaatgcgc cataaggtac ctggtaacga
     7441 gaagaacggc agttgttgtt cacgcaattg ctgccagaac tgacccacaa cctcttcgcc
     7501 acccagcagt tcacgcgctg cttttaccga tccttcgccg ccctcaaggc ggatccacaa
     7561 cgcattgtcg aagtaacata agccactaat gggtaatggc tggagttgcc actcggcgat
     7621 ttcactcatg gcttcttgca ggctgatttc ccgacgcagg ctcagagagg cacgcggtcg
     7681 cggtaacact ttcattgaga tttcagtgag cacgccaaga caaccgtagc ttccggccat
     7741 taaccgtgag agatcgtatc cggcaacgtt tttcatcact tcgccaccaa aacgcagatg
     7801 ttttccagtg ccggtaatga tgcgcgtgcc gaggacaaaa tcgcggaccg aaccgctcca
     7861 cgggcgacgc ggccccgcca gcccgcaggc gaccatcccg ccccaggtgg cttcttcacc
     7921 ataatgcggc ggctcgcagg gaagcatttg cccggcgctc tccagcgccg cttcaatcgc
     7981 caccagcggc gttccggcac gagcggttat caccagctcg gtcgggtcgt aattaacaat
     8041 gccgcgatga caacgcacat ccagcgtttg cccggtgact gggcgaccta aaaaggcttt
     8101 gctattgctg ccctgaatca ccagcggcgt tttatcgcta attgcctgat tcacctgctc
     8161 cagcagcgcc tgactgtaat cacactcgcg tagcatcaaa agcgctccag ttcaggaaaa
     8221 ggtaaatgac cgtgatgcac atgcatggca ccaaattcag cacagcggtg tagcgtggga
     8281 atgtttttcc cagggttcag caaaccatcg gggtcaaacg ccgccttgac cgcatggaag
     8341 gtcgtgattt catcgctgtt gaactgggcg cacatttgat tgattttttc tcgcccgatg
     8401 ccatgttccc cactgatgct gccgccaact tcaacgcaga gttcgaggat cttcccgccc
     8461 agctcttccg cgcgggcaaa ttcaccaggt tcgttggcat cgaaaaggat taacgggtgc
     8521 atgttgccat ctccggcatg aaagacgttg gcaacacgta aatcatattg ctgcgataaa
     8581 cgggcaatgc cttccagtac gccaggcagg gcgcgacgcg ggatggtgcc atccatgcag
     8641 tagtaatccg gggagatacg tcctaccgcc gggaacgcat ttttgcgacc ggcccagaaa
     8701 cgtacgcgct ctgcttcgtc ctgtgccaaa cggacgtcag tcgcgcccgc tttcaacaag
     8761 atgtagttaa cccgctcgca gtcttcctgt acgtcagact ccacgccgtc cagttcgcat
     8821 aacaaaatcg cttcggcgtc gacgggataa ccggcatgaa taaaatcttc cgccgcgcgg
     8881 atcgacaggt tatccatcat ctccagcccg ccggggataa tgccattggc gatgatgtca
     8941 ccaaccgcaa gtccggcttt ttctaccgag tcaaagctgg ctaacagaac ccgcgccacg
     9001 ggcggcttcg gcagcagttt taccgtcact tcggtggtca cgccgagcat accttccgat
     9061 ccggtgaaca gcgccagcag gtcaaaacca ggtgaatcca gcgcgtccga tcccagcgtc
     9121 agcgcctcgc cgtccagcgt ttgcacttca attttcagca ggttatgtac ggtcagacca
     9181 tatttcaggc agtggacgcc accggcattt tccgccacat tgccgccaat tgaacaggcg
     9241 atttgtgagg aaggatccgg cgcgtaatag agattatgcg gcgcaacggc ctaggagatc
     9301 gccaggttac atacgcccgg ctgcacgcgc gcgcggcgac caacggggtt aatgtcgagg
     9361 ctctctttaa agcgcgccat caccaacaac acaccttttt ccagcggcag cgcgccacca
     9421 gaaagcccgg tgcctgcacc acgggtcacc accggtacac gcaggcgatg gcagacagcc
     9481 agaatcgctg tcacctgttc catttgctta ggcagaacaa ccagtaatgg acgcgtgcga
     9541 tacgcgctca acccgtcaca ctcgtaagga atgatctcct catcggtatg caggatctca
     9601 agtccaggga catgctcacg cagtgccatc agtaccgatg tgcggtcgac atcgggtaaa
     9661 gcgccatcaa gacgctcttc gtacaagatg ctcatgagta ggcttcgctt tgttgtgttg
     9721 tgtggcagct gatttttgcg cgctgcttct gtgaacagtt attaagcggg cttttcgttt
     9781 tcgtctatct ctttagctac cggtcagacc attttttttc cagctctgtg accttgtctt
     9841 ggttaactta atgttaaatt gatgtaacat aatcacttgc gtgatgtgcg tgttttgcga
     9901 gttaagaaca gaaaaattgg tcctacctgt gcacgaggtc cgggaatgaa agatgaacgt
     9961 cgccctattt gcgaagtggt tgcagagagt atcgaacggt taattatcga cggcgtactg
    10021 aaggtcggtc agccgcttcc ctcggaacgt cgactgtgtg aaaagctcgg cttctcacgc
    10081 tccgcactgc gtgaagggct gaccgtgctg cgcgggcgcg ggattattga aacggcgcag
    10141 ggtcgcgatt ctcatgtcgc acggcttaat cgggagcagg acaccagccc gctgatccat
    10201 ctgttcagta cgcagccgcg aacgctgtac gatctgctcg acgttcgcgc attactggag
    10261 ggcgaatcgg caaggctggc ggcaacgctg ggaacgcagg ctgattttgt tgtgataacc
    10321 cgctgttatg aaaaaatgct cgccgccagt gagaaccaca aagagatttc gctgatcgaa
    10381 catgcgcagt tggatcacgc tttccatctc gccatttgtc aggcttctca caatcaggtg
    10441 ctggtgttta cgctgcaatc attgaccgat ctgatgttta attcagtgtt tgccagcgta
    10501 aataatctct accatcgacc acagcaaaaa aagcagatcg atcgccagca tgcgcggatc
    10561 tacaacgcgg tgttgcagcg gctgccgcac gtcgctcagc gcgcagcgcg cgatcatgta
    10621 cggaccgtga aaaagaatct ccacgatatc gagctggaag gccaccattt gattcgctcg
    10681 gcggtgccgc tggagatgaa caaggtggga atgtgagcag agttttaccc tatccccaag
    10741 gcgaggggac cgcccctgca cttaacagga tctggtgtta aatctgttca tctataccct
    10801 ctcccgaatg accgcccctg cgctaaacag gatctggtat taaatttgct tttcatacaa
    10861 tcggtaacgc ttgtacggct ccgccccaat gcgttccagc atgttattca tgcctgtatt
    10921 ggtttcgagg atccatgaca tctccagcgc atcgatcttc cggcgggcaa acggatcgcg
    10981 taaggcttca atcaataaca gcgcaatcac cgggccgatg cggctgaact gatactcgtc
    11041 gcgcacgccc atcagcggta ctcgcgcagt tcgcacaccg ctgactttca aacgccacag
    11101 caattttgcc cagccgaagg gaaagagcga tccgttcaga tcggcaatcg cctcgttgat
    11161 gttaggcaag ccacaataaa cgcgcagggt gcagaatcaa tctcagcgat gtagatcata
    11221 tcgtccggca ccagatattt aagttggtcg cccatggtcg cgaattcatg ttcggtaaac
    11281 ggcacaaatc cccagttgtg ctgccagcca gagttgaaaa tctcacgcag gatctgcatc
    11341 tcttcggcaa accgctgacg attgatgcag cgaatggtca cctttttgcg cacctgatcc
    11401 atcagttttt ttagcgccgg agagaaagtg agatcggttc gctgcatcca ccacgccagt
    11461 aaatcaatgc ctttgtgata acccagttgt tcaatatgcg cggcatacca cggtttgccg
    11521 tgtggcatca tcgcacaggg tggtgtgtca aaaccttcaa tcagtaatcc gctttcctga
    11581 ttgatattca ggctgaaagg cccgctgatc ttacttgcac cttgtgactt caaccacgct
    11641 tccgctgcgc caaacaacgc ggcaaaatcc tgcggatcat caatggcgtc aatcatgccg
    11701 aaatgaccgg tatcttcacc gtaacgctca cggtgcaagg tatctatttg cgcggtaata
    11761 cgcccaacta tctgccccgc tttttttgcc acccacgcct gccagatgat atggtccgtc
    11821 cccggatttt tcgcagacaa atgctcgttg cgttcaatga ataaaggggg tatccagttt
    11881 ggatcgtcgg gataaagtga tgacggaaaa gcgataaatg ccttaaggtc atttttatta
    11941 attacttttt caattttaat catcagcaga tcgcattcca tatctctgtg gataacaaat
    12001 aattaatggt gaaaaacagg ttgcaacagc aacgagcggc atcttaataa tcaactcaat
    12061 gaatacaaac gacgaataaa tagtattaat taatgattat gataaggtca gaggctacac
    12121 agaaacgcct caatgccttt tgtcaattta aggcgaccgg acaaggcttc ttcttaatat
    12181 attcataaat taaatatcat tcataataaa aacaaaaaac ttaaactcat tatttaaatc
    12241 aatgttcatt ttcacaccga tgaaaatact atctcattta tcttttatgc ctatataatc
    12301 caccgcatta ttgtcatttt atttttgata tccaattttt tcgtggtgta tatgtctaat
    12361 aaaatcttta cgcattccct acctatgcgc tatgccgatt ttccaacgct ggttgatgct
    12421 ttggactacg ccgctctgag tagagccgga atgaattttt atgacagacg ttgccaactt
    12481 gaagatcaac tggaatatca gacattaaaa acgcgtgccg aagctggtgc gaagcggttg
    12541 ttatcgctga acctgaaaaa aggcgaacgc gtggcgctga ttgccgaaac gagtagcggg
    12601 tttgtagagg ctttttttgc ctgtcagtat gctggcttag tcgccgtccc gttggcgatt
    12661 ccaatgggcg tcggtcagcg ggattcctgg agcgccaaac tccagggttt actggcaagt
    12721 tgccagcccg cagccattat cactggtgat gagtggttgc cactggtcaa tgccgcgacg
    12781 cataacaacc ccgaattaca tgttttaagc cacgcttggt ttaaggcatt accggaagcc
    12841 gatgttgcgc tccagcgtcc agttccgaac gatatcgcct acctccagta cacctccggc
    12901 agcacccgtt ttccccgtgg cgtcattatc acccatcgcg aagtgatggc taatctacgt
    12961 gctataagcc acgatggcat taaattacgc cctagcgacc gctgcgtctc ctggcagcct
    13021 ttctaccatg atatgggact ggtcggcttt ctcctgcccc ccgtcgccac gcaactttca
    13081 gtagattatt tgcgcactca ggattttgcc atgcgtcctt tgcaatggct taaattgatc
    13141 agtaaaaatc gcggcaccgt ttccgttgcg ccgccgtttg gctatgaatt gtgccaacgc
    13201 cgcgtgaatg aaaaagatct cgctgaactg gatctttcct gctggcgcgt cgctggtatt
    13261 ggtgctgagc cgatctccgc agaacaactc catcaattcg ctgaatgttt ccgtcaggtt
    13321 aactttgaca ataaaacttt catgccgtgc tacggactgg cagaaaatgc gctggctgtc
    13381 agcttctctg atgaagcctc cggggttgtg gttaacgaag tggatcgcga catcctcgaa
    13441 tatcagggta aagccgtcgc gccgggtgca gagacacgcg ccgtatcgac tttcgtcaac
    13501 tgcggcaaag cgttgccgga acatggtatt gaaatccgca atgaagcagg tatgccggtc
    13561 gcggaacgtg tggtaggcca tatttgcatc tccggtccca gtctgatgag cggttacttt
    13621 ggcgaccagg tttcgcaaga cgagattgcc gcgacgggct ggttagacac cggcgacctc
    13681 ggttatctgc tggacggtta tctgtatgtc accggacgca ttaaagatct gattattatt
    13741 cgtggccgta atatctggcc gcaggatatt gaatatatag cggaacagga accggaaatt
    13801 cattctggcg atgcgattgc ttttgttacc gcccaggaaa aaatcatttt gcagatccag
    13861 tgtcggatca gcgacgaaga acgtcgcggg cagcttatcc acgcgctggc ggcacggatc
    13921 caaagcgaat ttggcgtgac cgcggctatc gatctgttgc cgccccacag tattccccga
    13981 acgtcctccg gcaagcctgc ccgtgcggaa gcgaaaaaac gttatcagaa ggcttatgct
    14041 gccagtctta atgtgcagga atccctggca taaatcaaac tgtcgcggtg acgggcgcta
    14101 ccgggtttat cggtaaatat attatcgata acctgctcgc ccgcggcttt cacgttcgcg
    14161 cattgacgcg tactgcccgc gctcacgtca atgataatct cacctgggtg cgcggttcgc
    14221 tggaagatac tcattcactt agcgagctgg ttgccggagc cagcgtagtg gtccattgcg
    14281 ccgggcaagt gcgcgggcac aaagaagaga ttttcacccg ctgtaacgtt gacggcagcc
    14341 tgcgcctgat gcaagcagca aaagagagcg gcttttgcca acgttttctg tttatctctt
    14401 cgctggcggc gcgccatccc gagctctcct ggtatgcaaa atccaaatac gtcgccgagc
    14461 aacgactggc agcaatggcg gacgaaatta cgctgggcgt ttttcgcccg acagccgtgt
    14521 atggtcccgg cgataaagag ttaaaaccgc tgtttgactg gatgctacgc ggcctgctgc
    14581 cacgacttgg tgcgccagat acacaactct cttttcttca cgtcaccgat tttgcgcaag
    14641 cagtgggtca gtggttaagc gccgaaactg tacagacgca aacctatgaa ttatgcgatg
    14701 gcgtcgccgg tggctatgac tggcaacgcg tacgacagct tgccgccaaa gcccgttgtg
    14761 gttccgtgcg aatggttgac attcctctgc cggtactcac aggccttgcg gatatcagta
    14821 ccgcgttgag tcgcctggca ggtaaagaac ctatgctgac ccgctcgaaa attcgtgaat
    14881 taacccacgc cgactggtcg gcaagtaata accgtatttc tgaagatatc aattggtttc
    14941 ccgggattag cctggaacac gcattacgca acgggctatt ttgattgtct tgttttaaag
    15001 gtgtgttaac gtttctatgg taaatcgtga aatagtaatg gattacatcc tttcctgttt
    15061 acaggatttg gtcgaaaacg gtgtggaaat taagcccgac agcgatctcg tcaacgatct
    15121 tggcctggag tcgatcaaag tgatggatct cttaatgatg ctggaagaca gattcgatat
    15181 ttccattcct attaatattc tgctggatgt caaaacccct gcgcaattaa tggaaactct
    15241 acttccatgg ctggagaata aataatgggg ctatacgata aatatgcgcg ccttgctggc
    15301 gagcgtctgc aatttagtga caacgggtta acaccgtttg gcacctgtat tgatgaagtg
    15361 tattccgcca ccgaaggccg gatcggcaat aaaaaagtga ttctggccgg gaccaataat
    15421 tatctcggtt taacctttaa tcatgatgcc atcagcgaag gtcaggccgc gttagcggcc
    15481 caaggcaccg gcaccaccgg ttctcgtatg gcaaacggca gttatgcgcc gcacctggcg
    15541 ttagagaaag agattgccga gtttttcaat cgccctaccg ctatcgtttt tcaaccggtt
    15601 ataccgctaa tttaggcgtc atcagcgcac ttgccgacca taacgccgtg gtgttactgg
    15661 atgccgacag ccacgccagt atttatgacg tctgttctct cggtggcgcg gaaattattc
    15721 gtttccgcca taacgatgct aaagatctgg aacggcgcat ggttcgcctc ggtgaacggg
    15781 cgaaagaggc gattatcatc gttgagggca tctacagcat gctcggtgac gttgcgccgc
    15841 tggcggaaat tgttgatatc aaacgtcgtc tcggtggtta tttgattgtc gatgaagcgc
    15901 attcgtttgg cgtgttaggc gcaacggggc gcggactggc tgaagctgtc ggcgttgaag
    15961 atgatgtgga tattatcgtt ggtactttca gtaaaagtct ggcctcgatt ggcggttttg
    16021 ctgtcgtttc ggaagcgatg gaagtgctgc gctacggcag ccgcccgtat atttttaccg
    16081 cctctccttc tccgtcctgc attgcgaccg tgcgctcgtc gttaagaact attgccacac
    16141 aaccagagct gcggcaaaag ctgatggata acgccaacca tctgtatgac ggtctgcaaa
    16201 aactgggtta tgagttaagt tcacatatta gcccggtagt gccagtaata atcggttcga
    16261 aagaggaagg tttacgcatc tggcgcgagc ttatctcgcc gggcgtgtat gtgaatctta
    16321 tcctgccgcc tgccgcgcct gcaggcatca cgctgctgcg ctgtagcgta aatgctgccc
    16381 acagtcgtga gcaaatcgat gccatcattc aggcatttgc tactctgaag cagtaatgtg
    16441 agcccgccgt ggtgtttgcg ccacggcggg tttaatgttg tctgtcatac caatacacca
    16501 ggctcaacgc caccaacaca aacgctaccg gtaaaccgag cgcggtaaca ggtgcgctta
    16561 acgcaaatag caatcccaga ttaaccatga tttgatagct gatccaggtg agtaagccca
    16621 cgatgacgcc tacagcgaga cggctaccca tcccaggcga acgcggggca ctaaaggtaa
    16681 acggcacagc cagcaaaatc atcgccaggg tcaggatggg ttgccccagt ttttcccaca
    16741 gtgcaaggtg gtattcgctg ctgggttgcc cggtttcctg caaataatgg atgtaatgat
    16801 taagctgacg gacagaaaaa gtattccccg gcattgataa ctcttcaaga ttcatactgg
    16861 tgaaggccga ttgccaggca agattatcta atgtttccag cgtttctttg ccattcagcc
    16921 attttttatg attcacacca tgcaggaccc acgttttgtc gtcttcaatg gtggcactgc
    16981 gtgcataaat gtaggattca agcgagagat cgtcgcgata atgaaatatc tccacgccca
    17041 ccggctggcc ctgctcgttc agcgatttca ccgtcacaaa ttcattacca cgcctggccc
    17101 acagcatatt gccggtaatg tcatcgtcct cccccaacgc ggtggcggtc gatttgattt
    17161 gcaacgcctg ttgctgcaat ggcgacgcca cccactcatc tatcgcgcct aacgaaacag
    17221 tccacaatat cccggcaacc agcgccacca gtgcaatacg gaagatagaa aaccccgtgc
    17281 tgcgaatggc ggtaagctca ctgtttttcg ataactgccc cagtccaaca ataccaccca
    17341 ataacgcgat aaacggcgaa agttcgacca gtgtgcgcgg taaggtcatt agcaccacca
    17401 gcaccgcctg agtccagcga taaccgcccg ggctgacacc atccagttcg ttaatcaggt
    17461 tgaaggtggt aaaaagcggc agcaatagcc ctgcagcggc ggcaaaaccg agaaagagat
    17521 gacggattaa atagcgactg aaaacattca tcgggaaaat ttctgcagca aagagaagtc
    17581 acgtgcgacc agtatcagca ggcccattag catgagtccg ggcactaacc acaaaccagg
    17641 aatgagggga atcgcgccgt tagccaccag tgtacggcag acgtcgccgc cataaaaaat
    17701 ggcaataaac agcaacgtca gtggcaataa cgttgaaaat cgcccttgcc gcggttttac
    17761 ccgacttaat gaaatggcca gcagcgccat caacaatgcg gtcaggccac ggctttgtcg
    17821 ccattgcagt tcggcatgat cggcaggaaa ggcggagcgc gccagctccg tgaccgattt
    17881 cgctttgcgt ttgacgttcg ggctttgatc cagcggattc agatgtaatt gcagattacg
    17941 gtagatctgt tcgttgtcgt cacgcccctg atgatcgaga agataggcag tgccgttgtg
    18001 caacataacg gtcggtttct cgggtgatgg gtcaaccaca tcaaccgaac cggcgcggaa
    18061 aatacgggtt cgattggcag tagaagtata aatcagcgcg tcagtcaggc gattattatc
    18121 ctgatcaacc gtctgcgaaa ggatcattcg tccgttatcg ttagtgttaa atttctttgc
    18181 ccgcaactgg cgaacatcca gctccgactg cgactgctgc tccagttgat aaatctgcgc
    18241 ataagcccac ggacgaccat acatcgacag aagggtgaca aaaatactta gcggtatcgc
    18301 cagataaaga acggctttgt acaagcgtcc cggactgccc cccgccgcag agatagcggt
    18361 aatttccgag tcggtataca tctgccctag cgtcacgcca actgacacat acagaccaac
    18421 aggtaacaac atctccagtg caatcagtac tttgtagaaa acgatatcca gcacaacatc
    18481 aagagctaat gtcccatttg ccgcttcggt cagataacgc tgtgcggagt agctggcgaa
    18541 aataaagatc agaaaaccga cgataatcag caccagacgg cgcgtaccac gcatgatgta
    18601 gtgttcaacc agcttcatct cactaacgcc ctcaatcctt tgagtttttt attcattgat
    18661 cagtttctgc gccaacgcaa tgacatgatc tacctcttcc ggcgtcaggc tgccgtcctt
    18721 tgcccactga atttgcccgg ctttattcag caccagaatg gttgaactct gctccggcaa
    18781 gcgccaggca acgcgtccct gtccattgcc gtcaataacg aattgcgccc acggatagcg
    18841 gcgtttattt ttttcgattt tgccaacaac aaaatagcca gtaccaaaga tggcatcatc
    18901 ggtgttcaca atggtggtgg gctgaaaacg atcctgcgga aagtttgccg cttcaaccgc
    18961 tttaattaac agtgagttct ttttcttcgc cgacttacgc ccggcgatat actgaataat
    19021 gcggactttt cccgctaatt gtgtgctgtt ccacttttgg tagtgaattt cgtcgtcgct
    19081 attcagaagc agctcgccgc cttcctggat ataaacaggg gtaaccgttt tgccagtgac
    19141 gaaattgtgt gcctgcacac ccggggctaa gagcatgata atcaacgcaa taatcaaacg
    19201 agatgacatc attgctccgg ctatagcgga aaacgcgaaa tcagcagctc taagcgctga
    19261 caaatatcgc tatccagcgc caacggtgca taatacgact ggcggttttc catatcatcc
    19321 gccaccatgt cgtcgtgttt atctcccatc aggccattaa tggcggcgag gaaacgggaa
    19381 atctgtaacg cctctttatt ttcaggatca agcgatctta aggtgtcgta ataagaaacc
    19441 tgctggctga tgatttttcg cacatcatga aattccctgc cggttagtcg cggtttggca
    19501 ataatctctt tcagggtatg catttgcttt tgccgatagg caataaaact gcttacgctt
    19561 tctaactggc tatggcgtaa tgaatagttc accaacgacc agaccggaga gcttaaataa
    19621 atccgtaaca ggttgccgta atacgacaca atatttttct taccgttacg aaagccgtcc
    19681 tgaaaacgcc ccagaaaaac ggtcgttttg ccgaataaaa atccggcctg atgcttcttt
    19741 aaatacagcc gttgcgcaaa acgcaggtgc ttgtagcgcg ctcgcatgta tttaaattgt
    19801 ttgcgctcat cagcagtcag ctcatcgcct ttggcttgtt tgcgaactaa acgcagtaat
    19861 tcctgacgat caacgccatt gagccagaat tgcaggcata acgcgtaatt atcatgaatg
    19921 atttcggtgg tacaacttag tgcaatagtc ggcggcaaac taacctgtgc atcaacgaca
    19981 tcatcttctt caatcgcaac aaatagacgc tcgctcgcct caagcgggaa atattttctc
    20041 atcatcagga tttctctccg gctgcgttat aaagcaagga atggcgctga aaaaatgcct
    20101 ttaaggttgg aattaacgag agcgccacgt ccagacattg cccaccgaag taagcaaacg
    20161 ctgctcccag cacaccaaac agatgacaga acatgaacag caatacgatg tagccgattg
    20221 aggcgatggt ttgcgccacg agaaaagcgc gctgttttcc cgccatcaat agcaatgact
    20281 cctgcgggaa gcccagcatc gaaatcacaa ttgcgcccag catcacctga atcaggtcat
    20341 acgcttcgag atacttaacg ccaaacacca gcgaaatgag cggcttgccg acgatcaaca
    20401 ccgcgagcgc caccagaata ccgattccgc ccgccagtaa accggacttc acacctaaca
    20461 accacggtct ggtggtgcgc ggatctaaac gcatcacctc tgggtaaaaa cttttaccca
    20521 gcaaacccgc tggcgttccg gcggcgtcga agaatgtcat ggcgatttta aataacccgg
    20581 cggcagcggg tcctaacacg atccccacca acactgtgct gcacgagtta cgcgccgacc
    20641 agatggagtg ggcaatgttg gttgaccaga caaaactcca cgcgccttta atgtgtcggg
    20701 cagactcaaa cagattcaat ttgaaggcgt tatggatatt tctacggcgt aattcgcgcg
    20761 cggcaaacca ccagtacatg gtgccgccaa ccagattcga cacgtaccag gcaataacaa
    20821 aacccacaaa accaaagtca aaataccagg ctgcgacgct ccccgctgcg cgcagaaaag
    20881 gtttcgtcgc ctgctgtaca gcaatcaaat caaagcgatc taccgcacgc agaatgccgg
    20941 tcggcgtgga ggaagccatt gaaggaatga gcgtgcaata cagcgccgcc agccaaaagc
    21001 tttggtcatc cagacctaat gaatgggaaa ggaatggcag taaggcaatg ccgccgacaa
    21061 tcgccaccgc gccgctgacg atatccagcg agaaggaaaa tgagacgaca ttgcgaaatt
    21121 gctgcggatt attgttggtc aaggctggcg ttccgtactg aaccaccagc tgccatgtct
    21181 gaaacttaat aaaatcgctg atcgacttgg cgtacgattg cacaatcacc agtacaccaa
    21241 acatggcggg cgtcatccct ttaccggcac acgagagcgc caacagcccc agcaaggcgc
    21301 tctcgacatt actggagcct aaccaggcgc tattgcgaat aatggtgcga aacgcgccat
    21361 ctgcaaacca atgtttgatg ttaaaacccg ccaattcagc ctgaccttat cgttgatagt
    21421 aaaaaatatc ccgccagcct taagttaaac ttcggcggtc agaaacgatg gcaaccagag
    21481 aaaccgcctt ctgtacctgt tccagcactt cgctgtaggg cgctctggaa tcaatctcaa
    21541 gaatttttgt gccgttatag ccaatcgtcg acatgacgcc gattttgtcc tgcagctcgg
    21601 catactcatg gtcaggcttg cgggagatgg cagtatcaat atcaatcccc aaacgaatga
    21661 ttaattcagg acggtattgc gccatttttt ggtataaact ccgttcgcgc tgcgccagaa
    21721 acatgctgat tttcccggtc gcacgttcga cgccgatccc cggtccgtca taataaaagc
    21781 ccgaaatttc agcctgcggg aagcgatcgc tgaccaccag aacgccactt tgcgccagtc
    21841 gctgaacctt gcgtagattc gccattcttc gcagcgagaa gcagtacata atcaccgccg
    21901 cccatagcgc cggagatttg gttttcatgc tttgggtttt cgaggatttg gccgccaatc
    21961 gccgttccag ccagacgcca accaacggca atcgtttgat tttgtcgccg tcttcgccgg
    22021 agagcagccc cagatagcgg cgctcggttt gccagtgttg ttgcagcgat ttcaccaggt
    22081 cggtggtcag cgtggattta ccggtgccat cacacccgac caccgcaatc agccccggaa
    22141 tgtagttggg ctgcggcgcg gttgtactat taacagtttg tgtttgtagt gcatccattc
    22201 gttaaatcat cccctatcca ttttggtgat caggaaagcg cggcgtgaat tgcgcgtagt
    22261 gacgcttgca taatttcatc ggctggttgc cgaccatcca gttcaaggat cttggcgcca
    22321 ttgaatgtca gttgcggcgt aacggcgatt ttttcctgta acgctgccag ttggtggtca
    22381 ggtttacgcg caaacgcggt ttgttcatca atgccaagac gaatcaacaa tacgggtaaa
    22441 taagacgcca tccattggta cagcttcaac tcgcgctgcc ttaacatttt tatccaaccg
    22501 ttaccgcccg tggtttttgc caattgcggg ccatcaaagc gaaaacccgg cacttcaacc
    22561 tgcgggtagc ggtcggtgat gagcagatag ccttgctggc ttttacacaa cattttgcga
    22621 aacttgtacg cccgccagca ggaaagcaga tagataacca gcgcagtaat attgccaggc
    22681 ggagttaagg gcttttcgtg tacatgtgcc gctttacttc gcagataaca cccacaaggt
    22741 gcgccaataa cagggagctg tgaaatccat tcgccaattc gcccggacga ttgcccgaga
    22801 taaatgtgtt ctgttggcat tcttgctgcc agttcattta ccaggcttgc cgtgagggtc
    22861 gatttacctg aaccatcaca tcctacaatg gcaataacac gcactggcgt ggaattaatt
    22921 attgacatat tacgttgatt cacgaaaaac ccggcagtaa ataatgtatt gaatattacg
    22981 tggtccgtgc tcagaatatc cgttcaacct tattgtgaca atgactaatt gtgacattga
    23041 cctgaattac gctttcataa aaacatatta accaaataaa tatttttaat ggatatttaa
    23101 attaaaggat atattcatgc agtcaataac acctccatta attgccgtta ttggtagcga
    23161 tgcttcaggc aagtcaacgg tgtgtgaaca tcttattacc gttgtcgaaa aatatggtgc
    23221 tgccgaaaga gttcatttag gaaaacaggc cggaaatgtc ggtcgtgcag tgacaaaatt
    23281 acctttgatg ggaaaatcct tacataaaac aattgaacga aatcaggtga aaacagcaaa
    23341 aaaattgcct ggaccagttc cggcgctggt aattacagcg tttgtcgccc gtcgcttact
    23401 gcgctttcgt catatgcttg cctgtcgtcg tcgcgggtta attgttctaa ccgaccgtta
    23461 tcctcaggac caaattcctg gcgcttacga tggtacggtg ttcccaccta acgttgaagg
    23521 tggtcgtttt gtctcatggc tggcaagcca ggaacgtaaa gcgtttcact ggatggcgag
    23581 ccataagcct gatctggtca tcaaactcaa tgttgacctt gaagttgcct gtgcacgtaa
    23641 acccgaccat aaacgggaat cgctggcgag gaagattgcc ataacgccac agttaacctt
    23701 tggtggtgca caactggttg atatcgatgc caatcagcca ctggaacagg tgttggttga
    23761 tgcagaaaaa gcgattacgg attttatgac cgcgcgtggt tatcactagt caaaaatgga
    23821 aatgcccgat cgccaggacc gggcattttc aggaaggtta aatcaactgc aatgctatcc
    23881 agtacagccc accagaaaga aaaattgccg ccggtaaagt aaacacccac gccatcagga
    23941 tgctggttac cgttttacgc tgtaacccac cgccgtccac caccatcgtc cctgctaccg
    24001 cagacgagag gacgtgtgtt gtggagacag gcatcccaat ataactggca aggccgatag
    24061 acactgccgc cgtcatttgt gccgccatgc cttgcgcata cgtcatgccg cgcttaccaa
    24121 tcttctcacc gatggtcatc gctacacgac gccagccaat catggtgcca atgccgagcg
    24181 ccagtgctac cgccatgatg atccacaccg gagcgtactc aatggtgctt aacatatcgc
    24241 tgcgcagttt tttcagcagg ttctggtctt ctttactgac gcctggcagt ttcgccagct
    24301 tcgcggaggt atcggagatg cacagcataa tgcggcgcag ctggctgcgc tgactcacgc
    24361 ttaacggctc gtaactttcc atattgcctg gcagcatcgt tttaacgcgc gcaatcgcat
    24421 caaaggtatt tgccggatga cagtgaaact ctgttacttg cgtgccatca gtcgatgctg
    24481 caggcaatgg aggttccatc gcaatcaact tctgcggcag ttcaggatgc tgttgcaggt
    24541 agtgttcgaa gttggtaacg gcatcgcggg tacgggtaat ttcatagccg gacgcattca
    24601 tattgacgac gaagccagca ggggcaatcc ccaccagtac cagcattacc aggccgatcc
    24661 ctttctgccc gtcgttcgcg ccgtgcgaaa acgccacgcc cgcagcggaa acaatcagcg
    24721 caatacgcgt ccagaatggc ggtttacgtt tgcccttttt ctttttgcga tcttccggaa
    24781 tgcggtgaat acggtcacgc tttttcgtcc cactccagaa acggcgcagc aggaaaatca
    24841 gaccacccgc aatgaccagg ccgacgatag gggaaacaat cagcgaggag aaaattttgg
    24901 ttacttcacg caggtttaac gcatccatca ccgatgagcc ggttaacagc gcgttggtta
    24961 aaccgatgcc gataatcgca ccaatcaagg tgtgcgaact ggaggccggt aaaccaaaga
    25021 accacgttcc caggttccag ataatcgccg ccagcagcat ggaaaagacc atcgccaaac
    25081 cgtgagttga ccccatattc agcaacaaat cgattggtaa catatggaca atggcatagg
    25141 caacgctaag tccgcccaat aacacgccaa aaaagttaaa aaatgccgcc atcaccacag
    25201 caagttgtgg ctgcatggca cgagtataaa taacggttgc caccgcattc gccgtgtcat
    25261 gaaaaccatt gattgcttcg tagaacaaca caaatgccag agcaagtaat aacaaaagcc
    25321 ccgtatatat atcaaggcca acaaataaat ttagcatata ttagattacg ccattttgaa
    25381 tttacgaacg gacgcattat cagtgacttt aacggcatgg gcaaagtgaa atatcatttt
    25441 tttaacgata aaaagagtgt tatttcttgt gtatcagaca ataagcattc attcaaaacc
    25501 tttagaaata atcaaaacac aggtattcca ctggttaata tattcacata tgaaatgaat
    25561 aaatattgga atatataaat attgaatatt ttgattaatc cccgtactaa ttattcttca
    25621 taatcagtac ggggttgcta caacatgtat tactttttca ccacaattaa cggttcaata
    25681 tcactctctt ttttgatgac cagtgattca tcaccgcgca aacacgtccc accgtagttg
    25741 ccgccaacgg tgaaggtaca tacctgaatg tatttaccgt ccactttcgg caaacaccac
    25801 agttgctgat agatgttttt ctgctcggca aatttaccgc tggttttgtc cagcacctct
    25861 tcatgatggc tgaccaggtc gatattgcta ccacagcgac cggcgatcgg tttcactgcg
    25921 taacctgttt tcaccaattc atcattaaca gtgaaatcgg tatccagcag gtaacgatgg
    25981 tgcgggaaca gcgaccagag gatcggcaga atcgctttgt tgccggggat caccgtccac
    26041 agcggctcaa agaccagcac ttccgggcgc agcaatacgt cgataagacg cacttcgttt
    26101 tgcggatgac cggtacggat tggcaccgca gcaaactcac ggtcgctaac ttcacgaatc
    26161 tgatcaaacg cggtttccca cgcccaggtt ttccacacgc agttaaccag tcgcccttcc
    26221 ccatcaatca gttgcccggc agcatcccag cccagttcat ccagcccgcg caagatacgc
    26281 gtttcaaagc ccgcctggtg cagcgcctgc tccataaact gcgcgtgata gttttcctcg
    26341 atatctttgt cctgcatgat atggacaaac ggacgtgcac gactgtgttt ccaggcacca
    26401 gccagttcgt taatcagccc ttccgccgga ttgaagccgt tgcctttata gccctgctcc
    26461 gcccaacgtt cgaggatcaa gcccgcttcg gtatgacagg aggcggagtc ggcgttgtat
    26521 tcgtaaacct tcaggccacg ctcatccatg cagaaatcca tacgaccagt gatcatatgg
    26581 tgacggcgac gctgccagga gagacgcaaa cgtggccaga ggattttcgg aatgtcgaac
    26641 agcgccagca ggttgtcatc tttcagcacc ttgtcggttg cgtgaagata catcagatgc
    26701 agctcgttgg tggctttaat tagctcctgc tcggcactct cggtaatggt gtagtaatga
    26761 tacggatcct gattgatcac ctgaccgttg gcctgcacat aggcgttttg tagcggatct
    26821 ttttcatcca gccatttacc gtcaaactgg cctttgtttt ccagacgcgc tccactgatt
    26881 ttcagcagct cgcctgcaat ttccggctgc ggtaagctgt attcagtatc ttccgtctgg
    26941 atcatccagc ccagaatggt ggtgtcatca aaagtgtctt taagggtata gcagccgttt
    27001 tcgaccacca tctccagctc gcgcgtccac tgttgccctt gcggcaacgg ggaatgaatc
    27061 acgttctgtt ccgcaatacg gactttgttg ccatgcaatt gggtaatgat ggcgacatgg
    27121 ccagtgtctt taaattcacc gcctttatcc cagataagaa gcgcacccgc gaccggcgca
    27181 cgcggcgagc cgttaggaaa tgcctgcaat ggcaggatgt tgtcattaac cacttctcgc
    27241 aggaagcgca gcgagaaaat ctcccacgcc atacccacgt cagtaaagac cacaccgtaa
    27301 ttcagaaaga gaaaacggcg agcaaattca acgcattgcc acttgtggcc catatattcg
    27361 tcgtcgatat agctacggaa tacggcgtca tcttcgtatt cctgcggatc gagagaactg
    27421 taatctgaag agtagattgc taccccacct ggggcgtagc ccaataatgt cccgaacggg
    27481 gcatcctggc tggtcgttcc tttgctcatc actttacctt taacaataca acctaagcag
    27541 ttggcgtaat tgttgtgctc tgattacctg ctcagcgaca ttaaccggac agaggtcaga
    27601 ctaatcatac acctcatcgc agcggctggc gcaaagggtt aaaaaattca cattctgtac
    27661 agcaagtgac ctgctacact gcttcaacac taccactcag aaggcaactc actatgacag
    27721 acaatactta tcagcccgcg aaagtctgga cgtgggataa atccgctggc ggcgcgttcg
    27781 ccaatatcaa tcgcccgttt tctggtccga cgcatgaaaa aacgctgccc gttggcaaac
    27841 atccattgca actttattcg ctgggaacgc cgaacggtca gaaagtaacg attatgcttg
    27901 aggagctact ggcgctgggc gttactggtg cagagtacga cgcctggctg attcgtattg
    27961 gcgatggcga tcaattctcc agcggctttg tcgaagtgaa cccaaactcg aagatcccgg
    28021 cgctgcgcga tcatacgcat aatccgccga tccgcgtgtt tgaatctggt tcgatcctgc
    28081 tttatctggc ggagaaattt ggctacttcc tgccgcagga tctggcaaaa cggactgaaa
    28141 cgttgaactg gctgttctgg ttacagggtg ccgcgccgtt cctcggcggt ggttttggtc
    28201 acttttacca ttacgcgccg gtgaaaattg agtacgccat caaccgcttt accatggaag
    28261 ccaagcgcct gctcgacgtg ctggataagc aactggcgca gcataagttt gttgcgggcg
    28321 atgagtacac cattgcggat atggcgattt ggccgtggtt tggcaacgtg gtgctcggcg
    28381 gcgtgtatga tgccgcagag tttctggatg cgggaagtta taaacatgtg caacgctggg
    28441 cgaaagaagt gggcgaacgt ccggcagtga aacgtgggcg tattgttaac cgcaccaacg
    28501 ggccgctgaa cgagcagttg catgagcgcc atgacgccag tgattttgag acaaatacag
    28561 aagataagcg tcaggggtaa gggttggtgt tcgtcgcagc aagccatcca ggccggataa
    28621 ggcgttcacg ccgcatccgg caatcgtgca caatgcctga tgcgacgctg ccgcgtctta
    28681 tcaggcctac aaaagctaat catcacgcgc tggtaatgtc gtaatccatt tggcgcagtg
    28741 cgtctaatgt ggctttggct tcatcttcgt cgatgatgct catggcaaat ccgacgtgta
    28801 ccagcaccca ctggcccagc agatcggcag ggttaccttc gcaaatcagg gcgatattca
    28861 cgtcgcgctt gataccacat acttcaacct gcgcaagctg gtgaatatct tcaccgacag
    28921 ccagcacctg gcctggaacg ccaatacaca ttgttaactc cggttatttc gattgccgga
    28981 caaacgcgaa aaagaacctt tagcgggtgt tccctctcac cctaaccctc tccccggagg
    29041 ggcgagggga ccgtccgtgc gctttttagg cgttgtcctc agtcttattc aacttcaata
    29101 cttttgacga tcagcgaatc gccggtatcg acacgcaacc gctcgccgtg acagagcgga
    29161 cactgcgcat cgtgctgatg aatctccacc acctggctgc aatcccagca ccaggcctgg
    29221 gcgggtttat agacgatatg taaatcgcac ccttgcgcca ccgttccgtg gcagacaatt
    29281 tcaaaactaa aacggacggc gctctcctca acgcaggaga gcgcgccaat ttccagccac
    29341 acggcggtga cgcgcttaac atcgtgctgc tccgcctgcc gttggataat ttcaacggcg
    29401 ctctggcaaa gcgacaactc atgcattttc gcccctcctg cggccaaaca gcagggcgcg
    29461 acgccctgcg tgtggtacat ccggattggt gactggcaac gaaaggatca ttcgtgcgca
    29521 gtcatccgtc aggcgttggc cttcttcaat cgacatgctg tgcgaaagcg gcgacatcag
    29581 cgaacaggag aggtattgcg aaacaccgtc cagttcgcca acggtaaagg tcatggtgcc
    29641 atacggcagt tgcagaccaa ttttttcact cactttgcgc agcggccaga gttgatccgg
    29701 gccggggaag ataactgcac tcagcatcca cggggtgatc acacaccccg tccactgacc
    29761 ttcgaacagc gtaaaatcag aaacatacac cggcattgaa ggatgcagaa aagagagatc
    29821 gtgcatcgaa cgccgggcaa tttcttcaaa cgctgcctgt acttgcgcct tcggggaggt
    29881 ctggaaacct gctatctctt cagtcatgaa tcgcctcccg tgggatggct tccacgcctg
    29941 attcgcgcag cgcagccaga acctgctcaa gcgcaggttc aatcattgct tcaaccgtcg
    30001 gcgttaagcc gatgtgtggc tccagcgatt ccgggatcac gccgaccagg gtcagctttt
    30061 tcggaaactc accggtgaag cgcagggccg acaatacgtc ggccaggcca agctgatgcg
    30121 gagagatttt gttggtaaac aacgccggaa cttcttcatc ccgcaggatc atcatcgttc
    30181 ccggagtgtt ctttttcgac acaatggcat ccgcaataat cagatgatcg cgatttgcca
    30241 tgtcgccaag cagctccatt cccgccgtgc cgccatcgag gatctcaaca taatccggca
    30301 gaatgtatcg ttgctctaac gcttcgacaa tccgcacgcc gatggcttca tcggtcagca
    30361 aaatattgcc gacccctaag actaaaatac gcattacaga accttcactg aaaccacttc
    30421 gttgccgtcg gcatccacta cgtgtaccgc acaggccatg cacgggtcga aggagtgaat
    30481 ggtacgcacc acttccagcg gtttattcgg atcggcaacc ggtgtaccca ccagcgactg
    30541 ctcgtaagga ccgacgtcat cattgaagtt acgcggacca gagttccagg ttgatggaac
    30601 aaccgcctgg tagttgctga tgataccgtc tttaataacc atccagtgag agagcatacc
    30661 gcgcggcgct tcgaggaagc caacaccttt gaactcaccc gttgccggaa tgttcggctt
    30721 cacaaaggtg gtgtgatcgc ctttgccgat attggtgatc agtgcactgt attggttttg
    30781 caggatatcc tgcaattcac agcagtgaac ggtacgacca ataatacggc ccagcgtgga
    30841 gtgcagctgt gccacttcca gcgtgttgcc agtcagtttc tgataaatcg caacgatttc
    30901 attcagtttg ttctgggtag attcgcgacc tgccgccagt ttcaccagca tattagccag
    30961 cggacccact tctaccgttt tgccgtagaa agtcggtgat ttcacccagg aatatttccc
    31021 gtcgtcagac caaccatcat aagccggaat ggtggtacct tcccacggcg cctgtggcgc
    31081 ttcgtcttta taccaggagt gcttcgcgct ttcctgaatg cctttgatca ggtattcatc
    31141 ggaatgagaa gtgatcggac gatacgagga cagatccgca ttctcaatgt agcctcccgg
    31201 gaacaggaag ctgccgtttt tgctgtcggt cgggaattcc ggcacgctca ggtagttcac
    31261 cgcaccttta ccgcgcgtca gccattccgg gtagaacgcg gcgataaccg cggtatcaac
    31321 cttataaacc tgctcaacaa agtcgctcag tttgtcgatg aaagacttga tgtacatcag
    31381 gcgctcaagg ttcaacacgc ccaacccgtc gaggttgatt gggttcgcga caccacccac
    31441 agccaggttc tgaatgtgcg gcgttttacc gcccagcagc gccacgacgc ggttagcgtc
    31501 acgctggcac tccagcgcct gcaggtagtg cgctaccgca atcaggttca cttccggcgg
    31561 cagtttcatc gccgggtgac cccagtagcc attggcgaaa atacccaact gaccgctggc
    31621 aaccagatct ttgatcttgt tctgaacttt agtgaactct tccggactgt tcagatgcca
    31681 ggtcgaaacg cctttcagca tttcggaggc tttggttggg tcagcttgca gtgcagaagt
    31741 gatgtccacc cagtccagcg ccgaaagctg atagaaatga acgatatggt catgcgtggt
    31801 gtgcgcagcc agaatgatgt tacggatgta ttgcgcgtta accggaacgt cgatattcag
    31861 cgcactttct gccgcacgaa cggaagacag cgcgtgagtg gtagtacata cgccacagat
    31921 acgttgcaca atcatccatg catcgcgcgg atcgcggttt ttcacgatct cttccatgcc
    31981 gcgccacatg gtaccggaag cccatgcttt cgaaacgacg ccattttcga tttcgcaatc
    32041 gatgcgtaaa tgcccctcaa tacgggttac cggatcaata gtaattctct ggctcatgct
    32101 ttgctcgcct catgacgatt atgatcgttt tgttttaaag gaggaagtat cggcagtaga
    32161 cgaatgagta cgatgtaagc gcaaatctca atagccacaa aaccaataga aatcaacagt
    32221 tcttcccagg tcgggaagta ggcgtaaccg ccgcccgggt tgaatgccac caacgaatag
    32281 gtcagacgcc aggttgcaca acctaacagt gcgctcagtg ctgacaggaa cagcatacgg
    32341 gaatcattac gcagcttcgc cacacgcaga acgaccagcg ggaagagcat cagcaggact
    32401 tcaatccaga acatcacgga gtagaagtca ccggcaaacg ctaacgacag cttgtcgcga
    32461 tagataagct cgccaaagcg cagcccgatg aaaatcgcca gcaacacact gatggtgttg
    32521 gtcagcttga cgaacaggct cttttcatcc ggaccgttgc cacgcagacc cgcctgcacc
    32581 agtgaacctt caaagatgac aatcgagaag cccatgatga acgccgtcag cagcgagaac
    32641 agcggcaaca tttcatagct ctgccagagc ggatgcacct tgtagcccgc cgagatcatc
    32701 agcgacccca ttgaagactg gtgcatggtc ggcagcagcg caccgagcgc gatgatgaag
    32761 aacatcacct tgttcagacg ttgtagcgac accttccagc ccagacgttc aaacagtgcc
    32821 ggagcaaact ccagagccat cacgccgatg tagatggtca tacagaccgc cgtctcgaac
    32881 agtaccgagt tcacgttgaa gtgacccgga atgtagaagt acggcaggtt ccagtagcga
    32941 cccacgtcga tagtgatcga caagccaccc agtgagtaac caaacagact cgccaacagc
    33001 gccggacgca ccagcggatg gtattgccca cggttaaaga cgtataccgc ccacgccagc
    33061 gcccagccgc cacaggcaaa gccggtgcca atcagcaggt caaacgcgat ccacacgccc
    33121 cacgggaagc cgccgttcag gtcagagaca gagcccagac cgaacaccag acgcttcaca
    33181 atcaggagca tacagatgac gattaacggt ccaaaaatca tgaccggttt actgatgatt
    33241 ttgccgccca gcggttgtgg atcatgactc atgatcgtct cctccgtcgt gatggtcgtt
    33301 tttggtgttg cgacgaacca gcacggttaa gcccgccagc acagccagtg gtagcatcat
    33361 gcctttatac agggtgtgtt gaatattttc ggaacgcgca ccggtagaaa gatcgtccag
    33421 tttcggcagg tcgagatttt cataaggcac acccgtcagt accagaacct gagtaccgcc
    33481 gccctctttc tcgccgtaca gatgcggata atatttcggc accgtatgca ggtaagtgtc
    33541 gccagatttc agcgtctgac gcggatagtg gtattcgctg ccaggcttca gcgccagacg
    33601 ttttttcgcc tccgccatca gctcttcacg cgtaccgaaa atcaccgcgc ccgccgggca
    33661 cacttctacg cagccaggca gaccgccttt atcgagacgt tccacacctt tctggttgca
    33721 cagctcgcac ttatgcagcg caccaaacgg gttgttgtag tcgtacttcg gcacgttgta
    33781 cggacaggcg accatgcagt aacggcaacc ggtgcacacg tctttgtcgt aatggacaat
    33841 gccggttttc ggatcttttt tcagtgcaga gaccgggcac acagagacac agttcggatc
    33901 gacgcagtgc atacactgtt tcttaatgta cgcgtagccg ttctcctcct ggtctttgtt
    33961 gacccctgtg ccgctggtcc acacctgaat gatgttattg gtatacggcg acagtttgtc
    34021 gttgttcgac caggtctgtt ccccttgcgg gttacgttca gggaagttga tatcctgaca
    34081 cttggtgacg caagcctggc agcctacgca caaggtcgag tcgtacaaca tccccagcga
    34141 tcccggaatt ggcgggcggt tttcagcagc cgcatgactg acagacggca gcgcgcccgt
    34201 cagcaatgcc ccgcaggagg ctgctttaat aaaattacgt ctgttcacgg ttattctccc
    34261 cgtgagtcag cgttatcttt cttttgctga cgacccagtt cacgcaccgc catcacgctg
    34321 acaccggcaa ccagcccaac cacaccgccg agcaaaccaa tagcgcctgc agagacgttg
    34381 ccgccctctt tagcgttaac atccggtttc tgtgaacgcg gagtctgatt ttcgacgttg
    34441 gcaagctgat ggatgccttt atggaagccg ataccttttt cgttacagcc atagcacggg
    34501 tgaccaatcg ccaccggcca cacaccgcca acatcgcaga attgcagcgt tgagcagttg
    34561 ccgtaagttt ctggcccttt acagccgagg tggtacaagc accagccttc gcggtggcct
    34621 tcatcaccga actctttggc aaaacgacca gcatcgaagt gcgggcgacg ttcgcagtgt
    34681 tcgtgaatca gacggccata ggcgaaggtc ggacggtttt tgtcatccag tttcggcggt
    34741 ttgccgtaag tgatgatgtg cgcaacggtc gcgaggaagt tgtgcgggtt cggcgggcag
    34801 cccggaatat tgataacggt tttgcctggc agaacttctt gcaagctgac tgcgccagtt
    34861 gggttaactc cagctgcggc aacaccgccc cacgcggagc aggaaccaat agcgataatg
    34921 gctgctgagc cttccgccgc tttgcggatg tgatccacaa tcggctcacc ggcaaccatg
    34981 caataaatac cgttatcttt taatgggatg gaaccatcca ccactaacac atactgccct
    35041 ttgtacttct cgagagcgtt atgtttgttc tcttcgacct gatgaccgaa ggcggcggaa
    35101 agcacttcgt gatattccag agagatagtc tccagcacga ggttttctac cgttggatgc
    35161 gttgcacgaa gcagagattc cgtacaaccg gtgcactcct gcgcgccaat ccagataact
    35221 ggcggacgct gcgggttagt aaccgattcg gccatctctg cagcggcttt gctacttaac
    35281 cccatggtgg cggctaatgc tgcacaaagc ttcatgaaat cacgacggtt aatgccgtga
    35341 gaatggatga gggtgttatc tccagtcatt tatagttatt ccgttgcgag aacctggcat
    35401 atattttgcc tcaatcgcaa aatcaataat gcgatcgatg cgccatttac cacacattta
    35461 ttatggttat cgatatcatg atactgcggc ggggcgctaa aacgaaggga agaagcatta
    35521 gtgtaattaa ttaaagcaag ataatacgta tgtttgatca attttcgagg cgataaccgg
    35581 ccataaaaaa gcgcaacgta gaacaggaat tattctcacg aaagaaatcc attaccatct
    35641 ctttatccag tccatagcgg cgcgtcagaa tacccgcttc ccaggcgcta agctggcgat
    35701 ctccggtctt ttcgtacatc ctgtgtgagt agcctaatac aaaaccgcgt ttataatcgg
    35761 cacagaattt tgtcacatta accgcactgt cggcatgcgt tgcttttaat cccgccatta
    35821 agcctttacc aaaatggtta ttcatcgttc acctcattca ttatatatcg ccatatataa
    35881 cgatatttta ggcaaagaac gacttttgcg cacgctattt tttcatactt gtttaagacg
    35941 taatcccgta agagataaac ctatcaggag agatatttat cgaaccattt cagtgtcctt
    36001 tgccaggcaa gatcggcggc agatttgtca taacggggcg tggaatcatt atggaatccg
    36061 tgattaaccc ccggatagat ataagcctca taaaccttat tattgtcttt caacgccgcc
    36121 tcgtaaggag gccagccctc gttgattcgg gtgtccagtt ccgcgaagtg gagtagtaaa
    36181 ggcgcttcaa tcttcgccac atcggcagtg ggtgcctgac gaccataaaa cggcaccgcg
    36241 caggccagtt ccggatacgc gacagccgcc gcgttcgata cgccaccgcc atagcaaaat
    36301 ccggtaatac ccactttacc tgtcgcttgc ggatagcgtt gcataaactc aattgcggca
    36361 aagaaatcat tcatcagttt ggttggatca acctgctgtt gcagctcacg acctttatca
    36421 tcatttcccg gataacctcc aacggaactt aatccgtcag gtgccagggc gatatacccc
    36481 gccttcgcca ctcgccgtgc cacatcttcg atatacggat tcagtccacg attctcatgc
    36541 accaccccca cggctggcgt tttgccgctc atttttgcgg gtttcaccag ataaccccgt
    36601 acctcgccgt gaccatttgg cgaaggatac gtgatgtact cagcaacaat ctccgggtcg
    36661 gtaaactcta cctgagtcgc cagcgcataa tttggcttga gcaaatcaaa caacgctaat
    36721 gccgtcatcc cgcccaccgc atacttcgcc gcaaggttga ggaactcacg tttcgaaatt
    36781 ttcccgtgaa cgtaatagtc gtagtaatcc aacaactctt gtgggaaatc tttggcggtt
    36841 aaacgcggca tcgttgcatt cctcaactgg tgttttttta agcaaagcat aagcgcgtgt
    36901 ttttgcccag tttttcgtca ctctgtgagc cagactacgg gatacgcgct ggcgaatcgc
    36961 taaactagaa acattgtttc gaaattgaac ggtggaaagg agaggtcatg gtctggttag
    37021 cgaatcccga acgttacggg cagatgcagt accgctattg cggaaaaagt ggtttacgcc
    37081 tgcccgcatt atcgctcggt ttatggcaca atttcggtca cgttaacgcg ctggaatcac
    37141 agcgtgcaat cctgcgtaaa gcgtttgatt taggcattac gcactttgat ttagccaaca
    37201 attacgggcc gcctccagga agcgcagaag agaactttgg tcgcctgctg cgcgaggatt
    37261 ttgctgctta tcgcgatgaa ctgattatct ctaccaaggc tggctacgat atgtggcccg
    37321 gcccgtacgg ctctggcggt tcacgtaaat acctgctcgc cagcctcgac caaagcctga
    37381 agcgtatggg gcttgagtat gtcgatatct tttactctca tcgcgtcgat gaaaatacgc
    37441 cgatggaaga aaccgcctct gcgctggctc atgcggtaca aagcggtaag gcgctgtatg
    37501 tcgggatctc ctcttactcg ccagagcgga cgcaaaaaat ggtcgagttg ctgcacgagt
    37561 ggaaaattcc gcttttaatt catcaacctt cgtacaattt actgaaccgc tgggtggata
    37621 aaagcggcct gctggatacc ctgcaaaata acggcgtggg ctgtattgcc tttactcctc
    37681 tggctcaggg attgctgacc ggaaaatatc tcaacggcat tccgcaagat tcacggatgc
    37741 atcgtgaagg gaataaagtt cgtggtctga cgccgaaaat gcttaccgaa gccaacctca
    37801 acagcctgcg cttattgaat gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg
    37861 cgttaagctg gttgctgaaa gatgatcgcg tgacgtcggt attgattggt gccagccgcg
    37921 cggagcaact agaggagaac gtgcaggcgc tgaataatct gacatttagc accgaggagc
    37981 tggcgcagat tgatcagcat atcgccgatg gcgagctgaa tctgtggcag gcgtcgtccg
    38041 ataaatgacc tgttaataac gggcgcgcga accgcgcccg aatcagatca gtgattatga
    38101 cgagtcagtc ggtcaagata gcccatcaca aatgcagaaa gcacaaacgt cagatggata
    38161 atgacgtacc acatcagttt gttatccggg acatttttcg catccataaa gacgcgcagt
    38221 aagtgaatgg aagaaattgc cacaatcgac gctgctactt tgtttttcag cgacgttgcg
    38281 tccattttcc ccagccactt cagcttctct ttgttctcgg agatatccag ctgtgagacg
    38341 aaattctcat aaccggaaaa catcaccatc accagcaaac cgccaaccag cgtcatatcc
    38401 accagcgaca gcaacacgag gatcaaatct gattccgcca tcgagaagat attcggcagt
    38461 acgtgaataa tctcctggaa gaacttcagc gccagggcaa ctaacgccag cgaaaggcca
    38521 aagtacacgg gggcaagcag ccagcgagaa gcatacattg cattttcaag aaaacgttcc
    38581 atacattcct gtcgtgtagt taaaacgggc aacagtatat cgcaattgag taaacagcac
    38641 gcaacagcaa atcaacatat acgttaaacc tttgtcgcat aattctgagg tttagggatc
    38701 ttcctggttc ccttatcaac gcatcgagag cgtctaccct taaaggacgt tgaacctgaa
    38761 gggagaaaaa cgatgtctca tttaaaagac ccgaccacgc agtattacac tggtgaatat
    38821 cccaaacaga aacaaccgac gccaagcatc caggcgaaga tgacaccgat accggattgc
    38881 ggcgagaaaa cctatgttgg tagcggtcgc ctgaaagatc gtaaagcact ggtgacaggg
    38941 ggcgattccg gaataggtcg cgctgccgcc atcgcttacg cgcgtgaagg ggctgacgtg
    39001 gcgatcagtt atcttcccgt ggaagaagaa gacgctcagg atgtgaaaaa gatcattgaa
    39061 gaatgcggac gcaaagccgt tctgctgcca ggcgatttaa gcgatgagaa attcgcccgt
    39121 tcgctggttc acgaagcgca caaggcgtta ggcgggctgg atattatggc gctggtcgcc
    39181 gggaaacagg ttgccattcc ggatattgca gacctcacca gcgaacagtt tcaaaagacc
    39241 tttgccatta acgttttcgc gctgttctgg ctaacccagg aagcgatccc cctgctaccg
    39301 aaaggtgcaa gtgtcatcac cacttcgtca atccaggcat accagccaag tccgcattta
    39361 ctggactatg cggctacgaa ggcggcgatt ctgaactaca gccgtggcct ggcaaaacag
    39421 gtcgcggaga aaggtattcg ggtgaatatt gtcgcgccag gccctatctg gacggcactg
    39481 caaatttccg gcggacaaac gcaggataag atcccgcagt ttggtcagaa aacgccgatg
    39541 aagcgtgcgg ggcaaccggc ggaactggcc cctgtatatg tttatctggc aagtcaggag
    39601 tcgagctacg tcaccgcaga agtgcacggc gtgtgcggcg gcgagcattt aggttaaaaa
    39661 aatgcccggt tgtgaaaagc aaccgggcat cattgtgaat tcactcttac ctgatgtggc
    39721 gaaaacgcct tatccgccta caaacgcatg aagattcaac atgttgcaat agattttgta
    39781 ggcctgataa gcgtagcgca tcaggcattg agcacctaat gccggatgcg tcgtgaacgc
    39841 cttatccggc ctacaaacgc acgaagattc aacatgttgc aatagatttt gtaggcctga
    39901 tgagcgtagc gcatcaggca attctacttt acttcgcttt ggcggtttct tcgccgacca
    39961 gacctatctt caggtaaccc gcctgatgca gcgtatccat taccttcatc aacgtctcgt
    40021 aatcgacggt tttgtccgct cggaagaaga tggtggtgtc tttcttgcct tcggttaacg
    40081 cattcaacgc cgtaatcatt gtttcatcgg tgaccggatc gttaccgata aacatcgagt
    40141 tgtctgcctt caccgacaga taaaccggtt tttccgggcg cggctgcggc gtgctggtag
    40201 aagcaggcaa gttcaccttc acatctaccg tcgctaacgg tgccgccacc ataaagataa
    40261 tcagcagaac caacatcacg tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt
    40321 tatcgtcgag gttttcgtta agatgcattg ccatcggaca ttatcctgcg cgtaattttt
    40381 gtgcgacacg aaccggatgc gcagcggcgc tggcttccag atccaggtca cggctttgca
    40441 gcaacaatac ctgcgctgca acatcaccca gcatcgcttt aaagccgcca atctggcgtg
    40501 caaatacgtt atagataacg accgcaggaa tcgctgccac gaggccgatt gccgttgcta
    40561 acagagcttc tgcgataccc ggcgcaacga ctgccaggtt agtggtctgc gtttgcgcga
    40621 taccaataaa gctgttcatg atgccccaga ccgtaccaaa cagaccaacg aacggagaaa
    40681 tcgcgccgat ggttgccaga tagccgttac cgcggcccat ttgacgaccc actgcggcga
    40741 cccgacgctc cagacggaag ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt
    40801 ctgacagttc cagctcattc tgcgcttcat tgagcaaatg caggcttaag cttttgctac
    40861 caaaatcagc ggcgatatcg ttggcctggt ttaaggaacg tgcttcagcc agcagttgct
    40921 gctcgcgctt aaggcgacgc ttctgattga agaactctac gctcttactg aagaagattg
    40981 cccaggtgac tacagaggcc aaaataagcc caatcatcac gcacttaacg acaatatcgg
    41041 cgtactgata cataccccag acggaaaggt ccgtctgcat taaattatta cccactgtgt
    41101 atctccagga cgcaagtcac aaaatctgcg cataataata tcaaaacgac gtcgaattga
    41161 tagtcgttct cattactatt tgcatactgc cgtacctttg ctttcttttc cttgcgttta
    41221 cgcagtaaaa aagtcaccag cacgccattt gcgaaaattt tctgctttat gccaattctt
    41281 caggatgcgc ccgcgaatat tcatgctagt ttagacatcc agacgtataa aaacaggaat
    41341 cccgacatgg cggacaaaaa gcttgatact caactggtga atgcaggacg cagcaaaaaa
    41401 tacactcttg gcgcggtaaa tagcgtgatt cagcgcgctt cttcgctggt atttgagagt
    41461 atggaagcca aaaagcacgc gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg
    41521 cgcggaacgt taacccattt ttccttacaa caagcgatgt gtgaactgga aggtggcgca
    41581 ggctgcgcgc tatttccctg cggggcggca gcggttgcta attcgattct tgcttttgtc
    41641 gaacagggcg atcatgtgtt gatgaccaac accgcctatg aaccgagtca ggatttctgt
    41701 agcaaaatcc tcagcaaact gggcgtaacg acatcgtggt ttgatccgct gattggtgcc
    41761 gatatcgtta agcatctgca gccaaacact aaaatcgtgt ttctggaatc gccgggttcc
    41821 atcactatgg aagtccacga cgttccggcg attgttgccg ccgtgcgcag tgtggtgccg
    41881 gatgccatca ttatgatcga caacacctgg gcagccggtg tgctgtttaa ggcgctggat
    41941 tttggcatcg atgtttctat tcaggccgcc accaaatatc tggttgggca ttcagatgcg
    42001 atgattggca ctgccgtgtg caatgcccgt tgctgggagc agctacggga aaatgcctat
    42061 ctgatgggcc agatggtcga tgccgatacc gcctatataa ccagccgtgg cctgcgcaca
    42121 ttaggtgtgc gtttgcgtca acatcatgaa agcagtctga aagtggccga atggctggca
    42181 gaacatccgc aagttgcgcg agttaaccac cctgccctgc ctggcagtaa aggacacgaa
    42241 ttctggaaac gagactttac aggcagcagc gggctatttt cctttgtgct taagaaaaaa
    42301 ctcaatgatg aagagctggc gaactatctg gataacttca gtttattcag catggcctac
    42361 tcgtggggcg ggtatgaatc gttgatcctg gcaaatcaac cagaacatat cgccgccatt
    42421 cgcccacaag gcgagatcga ttttagcggg accttgattc gcctgcatat tggtctggaa
    42481 gatgtcgacg atctgattgc cgatctggac gccggttttg cacggattgt ataacattgc
    42541 cacttttgga caattttgca gacattttat tgtgaaaagt cttaaattgt tgcgtccggg
    42601 atcaaggcgt cccggacgat tcaggagtac aataggcaga taaaggctta aacgctgttc
    42661 cacaggaaag tccatggctg ttattcaaga tatcatcgct gcgctctggc aacacgactt
    42721 tgccgcgctg gcggatcctc atattgttag cgttgtttac tttgtcatgt ttgccacgct
    42781 gtttttagaa aacggcctgc tgcccgcctc atttttgcca ggcgacagct tgttgatact
    42841 ggcaggcgca ttgattgccc aaggggttat ggattttctg cctacgattg cgattctgac
    42901 cgccgcagca agtctgggct gctggctaag ttatattcag gggagctggt tagggaatac
    42961 caaaacggtg aaaggctggc tggcacagct tcctgctaaa tatcaccagc gcgccacctg
    43021 catgtttgac cgccacggtc tgctggcgct gctggctgga cgttttcttg catttgtccg
    43081 tacgctgctg ccaaccatgg cgggaatttc cggtctgcca aaccgccgct tccagttttt
    43141 caactggtta agtggattgc tgtgggtcag cgtggtaacc agttttggct atgccttaag
    43201 tatgattccg ttcgttaaac gccatgaaga tcaggtaatg acgttcctga tgatcctgcc
    43261 aattgccttg ttaaccgctg gcttgttagg cacgctgttt gtggtgatta aaaaaaaata
    43321 ctgtaacgcc tgacgatttt ccccgttccc ggctgctgta ccgggaacgt atttaattcc
    43381 cctgcatcgc ccgcattctt gccgcatctt cccccggcgt cacaccgaag taacgtttaa
    43441 actcacggct aaattgcgat gcgctttcat agccgacgcg catcgctgct gcgctggcct
    43501 tcatgccgtc atggatgatc atcatccgcg ccttatgcag acggtaattc ttcaaatact
    43561 gcaacggcga ggtgctggtg acagacttaa aattatggtg gaacgccgat acgctcatgt
    43621 tggcttctgc cgccagttgc tcgacgctca ggttttcggt gtatttattc tcaattcgtt
    43681 tcagcacgcg gctaatcaga ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc
    43741 cgccgcaagg tccggtcagc acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa
    43801 tacgcgcatc cagtggtcgc tccatcacgt cgagtaaccg ctccgccgcg cataaaatct
    43861 cttctgataa cgtggcggag ttaatcccgc tggctgccat cgacggctgg aaatgctcat
    43921 cttcgccaat gtccatcaac agttcctgta actgcaaaat atcgacattg agacgcaacc
    43981 ctgccagcgg cacctctgac gtggcatagg tttcgcactc aaacggcaac ggcaccgtca
    44041 gcagcaggta ttcattggca tcataacgaa acacgcgttc attgatataa ccgattttat
    44101 gcccggaaaa gagaattatg atgccaggct cgtacatcac cggtgtacgt gcgaaaggcg
    44161 tctcgccata caacaaacgc acatcgggca acagttctga caaactattt tctttatttt
    44221 tcagtttatt aactttatcc gccagcaagc gacaaatctc ttcacgtttc atatcgcgta
    44281 atttcttagg aataatgcgg caatttgatt gtgcgcaatt ttgtagcatt tctccagcac
    44341 tctggagaaa taggcaagac attggcagaa atgagcattg agagccaggg cgctggcgat
    44401 cacaatgaaa aacatcaggc agatcgttct ctgccctcat attggcccag caaagggagc
    44461 aagtaatgaa caactttaat ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa
    44521 tcgctggttt acgcgaacaa attcctcacg atgctcgcgt attgattacc tacggtggcg
    44581 gcagcgtgaa aaaaaccggc gttctcgatc aagttctgga tgccctgaaa ggcatggacg
    44641 tgctggaatt tggcggtatt gagccaaacc cggcttatga aacgctgatg aacgccgtga
    44701 aactggttcg cgaacagaaa gtgactttcc tgctggcggt tggcggcggt tctgtactgg
    44761 acggcaccaa atttatcgcc gcagcggcta actatccgga aaatatcgat ccgtggcaca
    44821 ttctgcaaac gggcggtaaa gagattaaaa gcgccatccc gatgggctgt gtgctgacgc
    44881 tgccagcaac cggttcagaa tccaacgcag gcgcggtgat ctcccgtaaa accacaggcg
    44941 acaagcaggc gttccattct gcacatgttc agccggtatt tgccgtgctc gatccggttt
    45001 atacctacac cctgccgccg cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca
    45061 ccgtggaaca gtatgttacc aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag
    45121 gcattttgct gacgctaatc gaagatggtc cgaaagccct gaaagagcca gaaaactacg
    45181 atgtgcgcgc caacgtcatg tgggcggcga ctcaggcgct gaacggtttg attggcactg
    45241 gcgtaccgca ggactgggca acgcatatgc tgggccacga actgactgcg atgcacggtc
    45301 tggatcacgc gcaaacactg gctatcgtcc tgcctgcact gtggaatgaa aaacgcgaga
    45361 ccaagcgcgc taagctgctg caatatgctg aacgcgtctg gaacatcact gaaggttccg
    45421 acgatgagcg tattgacgcc gcgattgccg caacccgcaa tttctttgag caattaggcg
    45481 tgccgaccca cctctccgac tacggtctgg acggcagctc catcccggct ttgctgaaaa
    45541 aactggaaga gcacggcatg acccaactgg gcgaaaatca tgacattacg ttggatgtca
    45601 gccgccgtat atacgaagcc gcccgctaag ctttttacgc ctcaaacttt cgttttcggg
    45661 catttcgtcc agacttaagt tcacaacacc tcaccggagc ctgctccggt gagttcatat
    45721 aaaggaggaa cgtatggcta atccaaccgt tattaagcta caggatggca atgtcatgcc
    45781 ccagctggga ctgggcgtct ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa
    45841 agcgttagaa gtgggttatc gctcgattga taccgccgcg gcctacaaga acgaagaagg
    45901 tgtcggcaaa gccctgaaaa atgcctcagt caacagagaa gaactgttca tcaccactaa
    45961 gctgtggaac gacgaccaca agcgcccccg cgaagccctg ctcgacagcc tgaaaaaact
    46021 ccagcttgat tatatcgacc tctacttaat gcactggccc gttcccgcta tcgaccatta
    46081 tgtcgaagca tggaaaggca tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg
    46141 cgtgtgcaac ttccagatcc atcacctgca acgcctgatt gatgaaactg gcgtgacgcc
    46201 tgtgataaac cagatcgaac ttcatccgct gatgcaacaa cgccagctac acgcttggaa
    46261 cgcgacacac aaaatccaga ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt
    46321 tttcgatcag aaagtcattc gcgatctggc agataaatac ggcaaaaccc cggcgcagat
    46381 tgttatccgc tggcatctgg atagcggcct ggtggtgatc ccgaaatcgg tcacaccttc
    46441 acgtattgcc gaaaactttg atgtctggga tttccgtctc gacaaagacg aactcggcaa
    46501 aattgcaaaa ctcgatcagg gcaagcgtct cggtcccgat cctgaccagt tcggcggcta
    46561 acgtgcaaat tctcccggtg gcggtaatgt tccgctaccg gacttttcag aaatcattta
    46621 ttcccctcgc gtcccgcccg ttgttactct tccttgttca ggaatgccaa atataaggac
    46681 atcatcatgc agagccggaa gctcttaaaa gaacaactca tctatatccg ggataaacgc
    46741 aacggagagg tgaaaaacag atgaaaataa tacttctgtt tttagcagcc ctggcaagtt
    46801 ttaccgtaca cgcgcagcct ccttcacaga ccgtagaaca aacagtccgg cagatttatc
    46861 agaactataa atcagatgcc agtacgcctt atttcggtga aaccggagag cgggcgataa
    46921 cttcagcgcg tattcaacag gcgcttaccc tgaacgacaa tcttacacta ccgggcaata
    46981 ttggctggct ggattatgat ccggtttgtg attgtcagga ttttggcgat ctggtgttag
    47041 aaagcgtagc gataacccaa actgacgtcg atcatgccga tgccgttgtg cgctttcgta
    47101 tctttaaaga tgataaagaa aagaccacac agacactgaa aatggtggcg gaaaatggtc
    47161 gttgggtcat tgatgatatt gtcagcaatc atggcagcgt cttacaagca gttaatagcg
    47221 agaatgaaaa aacgctggcc gctttagctt cgttgcagaa agaacagcca gaatcctttg
    47281 ttgctgaact ctttgaacat attgctgatt atagctggcc gtggacgtgg gtggtttccg
    47341 actcttaccg ccaggcggtt aatgccttct ataaaaccac cttcaagacg gccaataatc
    47401 ccgatgaaga tatgcaaata gaacggcaat ttatttacga caatccgatc tgttttggcg
    47461 aagagtcgct attttcacgc gttgatgaaa ttcgagtcct ggagaaaacc gccgattccg
    47521 cccgcattca tgttcgtttt acgctgacca atggcaacaa tgaagagcaa gagctggttt
    47581 tacagcggcg cgaaggtaag tgggaaatcg ctgattttat ccgcccgaac agcggcagcc
    47641 tacttaagca gattgaggca aaaaccgccg ccagattaaa gcaatgagct gaattaaata
    47701 acaattagcc ggaacaataa ataaaaggga acactatatg aaaacgattt tcaccgtggg
    47761 agctgttgtt ctggcaacct gcttgctcag tggctgcgtc aatgagcaaa aggtcaatca
    47821 actggcgagc aatgtacaaa cattaaatgc caaaatcgcc cggcttgagc aggatatgaa
    47881 agcactacgc ccacaaatct atgctgccaa atccgaagct aacagagcca atacgcgtct
    47941 tgatgcacag gactattttg attgcctgcg ctgcttgcgt atgtacgcag aatgataaaa
    48001 aaatccccgg cagcatgtca gttgccgggg atttttttta acgtccaacc gccgctttag
    48061 ggcgtttctt cgcaccagca ttcaccggac gagattgcgt agacgacgct ttttttgccg
    48121 tagcaggcgt ctgacgctga gtcgccatcg gcgtatgttt agtcaacgcc ggacgggtat
    48181 tgcggttctg gcgacgagct tcacgcatct cttcaatggt tggcgcaggc actaagcaat
    48241 cgcgacggct gccaatcaga tgctttttgc ccatcgcttc cagcgcctgg cggattaacg
    48301 gccagtttgc cggatcgtgg taacgcaaca acgctttatg caaacgacgc tgtttgtcgc
    48361 ccttcggtac gaagacgcct tcgctcttat aaccaatctt cgccagcggt tttttcccgg
    48421 tgtaatacat ggtggttgag ttcgccagcg gcgatgggta gaagttctgt acctggtcga
    48481 gacggaaacg atgctttttc agccacagcg ccaaattcac catatcttca tcacgcgtac
    48541 cggggtgcgc agagatgaaa tacgggatca gatactgctc tttacctgcc tgtttcgagt
    48601 aggtatcgaa cagctcttta aagcggtcat agctgcccat gcccggcttc atcatcttcg
    48661 acaacggccc ttcttcggta tgttccggag caatcttcag ataaccgccg acgtgatggg
    48721 tcgccaactc tttgatatag cgcggatctt ccacggcgat gtcataacgt acgcctgagg
    48781 cgataaggat ttttttaatg cctttcagat cacgcgcacg gcgatagagg ttgatcgtcg
    48841 gttcgtggtt agtgtccatg tgcggacaaa tatccggata aacgcacgac aaacggcgac
    48901 aagtttgttc agcgcgtggc gatttgcagc gcaacatata catgttggca gttggcccac
    48961 cgagatcgga aatcacgccc gtaaaacctg gaacggtgtc gcggatcgct tcgatctcat
    49021 taatgatcga atcttccgaa cggctctgaa taatgcgccc ttcgtgctcg gtaatagaac
    49081 agaaagaaca gccgccaaag cagccgcgca taatgttgac cgaaaaacgg atcatttcgt
    49141 aagccggaat acgggcattg ccataggccg gatgtggcac gcgcttgtac ggcagcgcaa
    49201 aaacgctgtc catctcttcg gtagaaagcg ggatagcagg cgggttgatc cacacatagc
    49261 ggtcaccgtg tttttgcatc aatgcgcggg cgcagcctgg gttggtttcg tggtgcagaa
    49321 tgcgcgaagc atgggcgtac agcactttat cgcccttcac tttctcgaaa gaaggcagca
    49381 atacgtaggt tttttcccac ggtttctggc gcggtggctg cacgattaca gctttcgctt
    49441 cctgcttttt cggcgcaaca ggtttgttat ccgcacacgg caaatcttcg ccatacggat
    49501 gcgggattgg gtcgattttc cctagggtat caagacgggt ggaatccaca ccgctccagc
    49561 caggcagcgc ctcttttacg ataattgcgg tattacgcac atcgcggatt tcactaatcg
    49621 gctcgcccat cgccagacga tgcgctactt ccaccagcgg acgttcaccg ttaccaaaca
    49681 tcagcatgtc ggctttcgaa tctaccagaa cggaacggcg cacggtatcg gaccagtaat
    49741 cataatgcgc ggtacggcgc aggctagcct caataccgcc gaggatcacc ggcacatctt
    49801 tccacgcctc tttacaacgc tgggtataaa ccagcgtggc gcgatccggt cgcttacccg
    49861 cgacattatc tggcgtgtag gcatcatcat gacgtaaacg gcgatcggcg gtatagcggt
    49921 tgatcatcga atccatgttg ccagcagtaa caccaaaaaa cagattcggt ttacccagac
    49981 gcataaagtc gtctttgctg ctccagtccg gctgggcgat gatcccgacg cgaaagccct
    50041 gcgcttccag catacgaccg caaatcgcca tcccgaagct tgggtgatcg acatacgcgt
    50101 cgccagtaac caaaatgatg tcgcagctat cccagccaag ttgatccatc tcttcacgag
    50161 acatcggcaa aaacggtgcc ggtccaaaac aggcggccca gtactgcggc caggagaaca
    50221 ggtcgcgatc cggttggatc agggagatag agctcatttt gcttccagaa atgataaaaa
    50281 aataatcaaa ggccggggat tataagccgg aacgaaagag aaatcgaaag gtattccata
    50341 ctcgccctcc tcgggcgagt atgaagatta cggtaccgga ttgaccaaca gttgcccaat
    50401 cgagccacgg tccgccattt ccagcgtctg actgttgaag tagaacggga agtgcgccca
    50461 ggaaggctga ccgaaataaa caagcagctc cacttgtccg tcaacccaaa cggtatcttt
    50521 ccagcctctg tcttccggga acggcatcgc gccattcacg ttacggatct ggaacattac
    50581 gccttcaata tggaacgctt gcggctcgtc cgcgcgtacc gtccagcgtt cccacgttcc
    50641 ttgctgcgcg gtgacatcaa tacggttgac gtcccacagc tgtccattaa tacccgggtc
    50701 atcacccaga ctgatatcgc gactgcgaat tggcgaacca gccatgattt cagttggcag
    50761 caagcgcatc ggaagactgt ctgtgaccag cggcagaagg ccggttgggc gcagcgttag
    50821 caccaaggta gaaaccagaa tactggatgg ctcaaagaag ccgcgaatac gatcaacaat
    50881 gctcgccgct tcaccacagg tgatcgacac ttcatcgccg ttgctcatat ccaccagaat
    50941 ctcgcggcgc tcgcccggtg ccagcgaaag ttgcttcacc gatacaggag caggcaggaa
    51001 tccctgatcg ccagaaatca catgtaacgg gcgaccatcg ctcatctgta gttgatagcg
    51061 acgagagttc gacgcgttca gcagtcgcaa gcgcacccag ccacgcgaga cttcaacgta
    51121 cgggctttgt acaccgttaa ccagcagcgt atcaccaaca aagccgccgc ttcccggttc
    51181 gttgtattct ggcgtaccaa agttatccag ccgtttatcc tggataatga ccggaaaatc
    51241 atccacacca taatggttgg ggataggcag cgacttgctg acttcatctt ccaccagcca
    51301 cattccggca aggccgttat agacctgctg agccgtgcgg ttgggagtat tggcgtgata
    51361 ccacagagta gctgcgttct ggcgaatagg cagtacgggt gcccagtcag cgtttggcga
    51421 catcatccgt gccggaccgc ccatcagcgg gcctggtacc tgtagcccgg ccaccgtcat
    51481 tgagacattt tctgtcaggc ggttgctgta aataagctta acatcgtcgc ccttccagac
    51541 gcggatagtc ggccccaggt aacgaccatt gattccccag accgacgcgc gtgtccctgg
    51601 cgtaaatgac cagtgcgcac gttgtacagt cataaacagc ggttgcccac ggcgagattc
    51661 aagtagcggc ggaacgggta gcggttgctg ttgcccggct gcgctggcct tcaggggaac
    51721 agcgcctgca caaagtgcaa tccccgatgc ctgaatgaac tgacgccgac tgagtgacat
    51781 atttgctcca tgtaaaactg gctaaagtaa taccgtgcag gaaagtgttc cccgcaaaaa
    51841 tacagattgc cgacttaaac ttttccggcg gcttcgcgtt ctgcgacttc tttatcgagc
    51901 tcggcgattt tttgttccat tatcgaacga caatgggcag ccagctcacg aacctgatct
    51961 ttgccatact gactgacgtc aattggcggc agcatttcga caatcaccag accgttgtgc
    52021 agtcgattaa gattaatctt attcgaagtt gtagagacgc acacgggaat aatcgggacg
    52081 cccgccgcaa ttgccgcgtg aaatgctcca gtcttgaacg gtagcaggcc gcgaccacgg
    52141 ctgcgggttc cttccgggaa catccagatg gaaatacggc gttttttgaa atgattcacg
    52201 acttccgcaa tggtgccgtg agctttagtg cgattgtttc tgtcgatcaa taagttgccg
    52261 gttaaccagt acaactgccc gaagaagggg atccacagca agctcttttt acctaccgtc
    52321 accgtcggcg gttgcacaat gttcgatgct gtcaccatgt catagttgtt ctggtggtta
    52381 gcgatataga tagcattgcc gtagctttca gcatctgcag gtttgcggca ctcaactttc
    52441 aggccgaaca gtggcgcaag acggccaaac atatgcccaa aggtggccac atgtttcggg
    52501 ttacgcgggc tgaaaaggca gtaaatggag ccgaatacac agactaagat gctgtaaatc
    52561 acggtaataa taagacgaaa gatatatagc ataacaccct ctgaaggtct gagagactgg
    52621 cattgtacgt cacctgaaat caggttaggg cattgttaaa agccgctctc tggaaaagcg
    52681 tattgttaat cgcaacgcat gaataaacaa cggtttcaag ggaattttta tcgtttccgg
    52741 gggttgaata cgctgccgga tgacgactta acgtttcatc cggcgttcct tgctagcggg
    52801 aggaaacagc gccctccccg gcatattact cttcgctatc accgctgctg gcacggcgag
    52861 gagagtcgat ctcaacacga tcgatacgct gcaaaccgcg catcaacgta ccgcggcgtc
    52921 cacgttcgcc agtgactttc tgtagctctt ccggacgcag tttaattttg cgtttcccaa
    52981 catgaatggt cagcgtgctt tgcggcggca gaacgtacag ttgcgccaga ccatcctcgc
    53041 cacgcgcggc ttctgccgat ggaatgttga taatcttgtt gcctttgccc ttcgacagct
    53101 gcggcagatc gcttaccggg aacatcaaca tacggcctgc ctgagtgatt gccagcagca
    53161 tatcggaagc atcttcaatc accaccggcg gcataacatg ggcattttcc ggtaaggtga
    53221 tcaaagcctt acccgcacgg ttacgcgcta ccagatcgtt aaaggtgcag acgaaaccgt
    53281 aacccgcatc ggaagccatc agcagtttct gatcgtcgct ttccatcagc atatggtcaa
    53341 cggtcgcccc aggcggcaac gttaatttgc cggtgagtgg ttcgccctga ccacgcgccg
    53401 acggcagcgt aatcgggtcg atggcatagc tacgaccggt ggaatcaaca aacactaccg
    53461 gttggttact cttacctttc accgccgctt tgaagctatc acccgcttta taattcaggc
    53521 ccggcgcgtc gatatcatgg cctttagcgc tgcgtaccca gcccatctgc gacagcacaa
    53581 tggtgacagg ttcagacggc agcatgtcgt gctcgctcat cgctttcgct tcttcgcgtt
    53641 cctgcaacgg cgaacgacga tcgtcaccgt aggcttgcgc gtctgcctgc agttctttct
    53701 tcagcaggtt attcatttta cgctcggaag ccaaaatgcc ctgcaactgg tcgcgctctt
    53761 tttccagctc actctgctca ccgcgaatct tcatctcttc cagtttggca agatgacgca
    53821 gtttcagttc gaggatcgct tccgcctggg tttccgtaag gccaaaccgc gacatcagcg
    53881 ccggtttcgg ttcatcttcg ttacgaatga tctcaatcac ttcgtcgatg ttgagaaacg
    53941 ccaccagcaa accttccagg atatgcaggc gcttgaggac tttctccaga cgatagttca
    54001 gtcggcggcg cacggtatcg cgacggaaca ccagccattc ggagaggatt tccagcaggt
    54061 ttttcaccgc cggacgacca tccagaccga tcatattgag gttaatgcga tagctctttt
    54121 ccagatcggt ggtagcgaag aggtggttca tcacctgatc catatccacg cggttggaac
    54181 gcggcacaat caccagacgg gtcgggttct cgtggtcaga ttcatcgcgc agatcgtcaa
    54241 ccatcggcag ctttttgttg cgcatttgcg cagcaatttg ctccagtacg cgcgcacctg
    54301 aaacctgatg cggtaatgcg ctgataacca ccgcgccatc ttctttcttc cacaccgcgc
    54361 gcatacgcac tgaaccacgt ccgttctcgt agattttacg aatctcggcg cgcgaagtga
    54421 taatttccgc ttcagtcgga taatccggcc cctgcacgat atccagcagc tgatcgagcg
    54481 tagttttcgg ctggtcgatt aatgcgattg ccgcctgagc tacttcacgc aggttatgcg
    54541 gtggaatatc ggtcgccatg ccgacggcaa taccggtggt gccgttaagc aaaatgtttg
    54601 gcagacgggc aggtagcatt ttcggctcct gcaaagtgcc gtcgaagttt ggcacccagt
    54661 cagccgtccc ctgccccagc tcgctcaata gcagctcgga atatttcgac aaccgggatt
    54721 cggtgtaacg cattgccgcg aacgatttcg gatcgtccgg cgcgccccag ttcccctgac
    54781 catcaaccag cggataacgg taagagaacg gctgcgccat caggaccatc gcttcataac
    54841 aggcgctatc gccgtgcgga tggtatttac ccagtacgtc accgacggta cgggccgatt
    54901 ttttaaattt ggcgctggca ttcaggccca gttcagacat cgcatacaca atgcggcgct
    54961 gaacaggttt cagaccatca ccaataaacg gcaacgcacg gtccatgatc acgtacatgg
    55021 agtagtttaa gtaggcgttt tccgtaaatt catgtagcgc aaggcgctct gccatatcgc
    55081 tcattaattc tgattcctca acttattcgc cagccttcaa aggggcaata ttgccggaga
    55141 tactacatta tctgccgcgt cgagtcacag gagatcagcg tgaatagcgc cgccggacgt
    55201 gctccgacgg cgataaaggc atcaatgtgc cttgatatac aactctttgc tgtagacttg
    55261 atcggcatag agggcagcac aaggttgcgc ccatgccaca ccactcggca tgggggtccg
    55321 ctccgggcga ttcgagaagt tgaggttatg cgagtccagg gaagcctaaa gcatcaaagt
    55381 agtcgactgt aagtatacgg ttcagcccag tttagctgtg tctccaccag tgccccgcgc
    55441 ctcctgccat aagtgcggct tgctcatgga tatccccgag acggcttacg gcgcaccgga
    55501 taataaattt cgcgtataca tggtattcct taccggagaa attataagcc caacaacaat
    55561 acgcctgtga cattgttcgc tcaaacttat cgcgagtgat ttggctcaca ctccggtaaa
    55621 gattccccat cacgccaacg gcacataaac atccgtttcc agtcgctgct ctgtcacccc
    55681 ttcggcaaga ttggtgtagt gaaacagaat cggtgcttta cgcattttct cgccgcttgc
    55741 aggcagccag tggcgaataa tgccccatat cgtgtgtgaa atatcgtcca gctcgccaac
    55801 gtggcgggcc acggcataac gtccaccggt aagttcacca ttgctcacac cataacgatt
    55861 atcgggaatc agttcgctaa cgctgccgca gatatcaaag cgaaacgctt ccggtgcggt
    55921 ggtggcaggg gcgtcccagg cgacgccaaa agtctggctt tggttaacag gagaaagccc
    55981 cgtctcttta cgccacatga taaatttcgc cacactgtag ttgagcaact cagggctaca
    56041 gcggtgcggc aacatcgcca cagggattga tgggaaatcg ataatgttta catccagtgt
    56101 caggtttgtc attttgcctc cctgcgaaat tattcccggg aacgcgtccc gggagcggta
    56161 acaattacgg atttactttg cggatctgtt tgacgtcaat ttcaacagaa ttccagtctt
    56221 tatcgacttc accctgaatc tcaaccgtat ctttcggcgt caccgtcaca ccgttccagc
    56281 gtttgtgatc gatatcaaca ttgatagtac cgctggcatc tttaaacacg tagagatcgt
    56341 cagagatgcg ttcaacgata ttgccgcgca gggttaccca ggtgtcgtca cgcagggatt
    56401 ttgcgctttc tacagtcgtt acgctgccgt tcggcccctg aaatcctccg gcctgacttt
    56461 gcgttaccga tgggccagaa aaaccgccct gctctgctgc catcaccggt gcactgcaca
    56521 gggccattac tgcgattact gctgcgaatt ttttcatgtt tattactccc tttaatgtct
    56581 gtttccgagc atttaacaag atagtcctta acaacttctt aagggaaaaa aataaaattt
    56641 aatgctgtac agagcgcgtt acaacacggt ttactggcag caaatacggt tatcgcaggg
    56701 atgaaaaaat gcgaatttta ctgatagaag atgacatgct gattggtgac ggcatcaaaa
    56761 ctggccttag taaaatgggt tttagtgtcg actggtttac acaaggtcgt cagggaaaag
    56821 aggcgcttta tagcgcacct tatgatgcgg tgatcctgga tttaacctta ccaggcatgg
    56881 atggtcgcga tattttgcgc gaatggcgag agaaaggtca gcgtgagccg gtactgaacc
    56941 tgaccgcgcg tgatgcgtta gcggaacgtg tagaagggct gcgtctggga gctgacgatt
    57001 atctgtgtaa accttttgcg ttgatagaag tcgccgccag gctggaggct ctgatgcgcc
    57061 gaaccaacgg ccaggccagc aacgagctgc gccacggtaa cgtcatgctc gaccccggca
    57121 aacgtatcgc cacgctggct ggcgaaccct taacactgaa accaaaagaa tttgccctgc
    57181 tggaattact gatgcgtaac gctggtcggg tactgccgcg caaactgatt gaagagaaac
    57241 tgtatacctg ggacgaagag gtcaccagta atgccgttga agtgcatgtg catcatctgc
    57301 gacgcaaact cggtagtgat tttattcgta ccgtgcatgg tattggctac acattaggtg
    57361 agaaatgaaa tttacccaac gtcttagtct gcgcgtcagg ctgacgctaa tctttttaat
    57421 tctggcctcg gtgacctggc tgctttccag ctttgtcgcc tggaaacaaa caacggataa
    57481 cgtcgatgaa ttgttcgaca cccaactgat gctgtttgcc aaacggttaa gtacgctcga
    57541 tctcaacgaa atcaacgcgg cggatcgcat ggcacagacg ccaaataaat taaaacacgg
    57601 tcatgttgat gacgatgcgc tgacctttgc catctttacc cacgacggca gaatggtcct
    57661 taatgatggc gataacggag aagatattcc ctatagctat caacgggaag gttttgctga
    57721 cgggcaactg gtcggtgaag acgatccttg gcgttttgtc tggatgacct cacctgatgg
    57781 caaatatcgc atcgttgttg gccaggaatg ggaataccgt gaagacatgg cgctggcgat
    57841 tgttgccggg caattgatcc cgtggctggt cgcactgccg attatgttaa tcatcatgat
    57901 ggtactactg ggtcgtgaac tcgcgccgct gaacaaactg gcgctggcac tacgtatgcg
    57961 tgaccctgac tcggaaaaac cactaaacgc gactggcgta cccagcgaag tgcggccact
    58021 ggttgagtcg ctaaatcaac tgttcgcccg cacacatgcg atgatggttc gagaacgacg
    58081 ctttacctcc gatgcagctc acgaactacg tagcccgtta acagcgctga aagtgcaaac
    58141 cgaagttgcg cagctctctg acgatgatcc gcaggcgcgg aaaaaagcac tgctccaatt
    58201 acattccggg atcgatcgcg ctactcgtct ggttgatcaa ctgctcacgc tatcgcggct
    58261 ggactcactg gataaccttc aggacgtcgc ggagatcccg cttgaagatc tcctacaatc
    58321 gtcggtgatg gatatttacc acacggcgca gcaggcgaaa attgacgtgc gactgacact
    58381 caatgcccac ggcatcaaac gcaccggaca accgctattg ctaagtttgt tggtgcgaaa
    58441 tttgctggat aacggccccg gtgtgacacc agaggcactg gcgcgaattg gcgaacgctt
    58501 ctatcgccca cccggacaaa ccgctaccgg cagtggactt gggctatcga ttgtccagcg
    58561 aatcgccaag ctgcatgaca tgaatgttga gtttgggaat gcggaacaag gtggatttga
    58621 ggcgaaggta agctggtaac ttctctgtct atgagagccg ttaaaacgac tctcatagat
    58681 tttactaata gcaaaatata aaccgtcccc aaaaaagcca ccaaccacaa accagacaaa
    58741 taacgagcga gaatacatat agccaaaact cgaacatttc ccgcataaag agttttctta
    58801 agataagaat aataagtggc gtaagaagaa aaaatgctgc aatcattgct tgcaatatac
    58861 gatcttcgcc tgaaacggaa ggtatactgc aaatattgcc tcccgactca agcatccagt
    58921 catacttatt gaaagcaaaa aaagaatcta ttaaccaata agtaagcatt atatatagca
    58981 atgcttcaaa tattgtttta attttctgct ttaacatata tcagtacgct cataaaaatc
    59041 catttataat tatcaacatg acacgttgat attttcatct tttacttatt caaagccata
    59101 ttttcaatcc ccaaaaaaac cgaaccaaac gtgtaaatta ttccggtgcg agtaagaatt
    59161 atgaggaatg gctatcagta ttgtcatttt cagaaaatat ttatcctgca tcggtgagtc
    59221 agagctagat cagacttttg ctaaattcgc aaaagacttt gcacattttg ctaatttcac
    59281 agtaccgctc tgtgacgtac tatagtcgac aaacgtctca ccttgagatt aaaaaatgag
    59341 caacatcctg attatcaacg gcgcgaaaaa attcgcccac tccaatggtc aactgaacga
    59401 caccctgacc gaagtcgcgg atggcacact gcgcgacctc gggcatgatg tccgcatcgt
    59461 tcgcgccgac agcgactaca atgtcaaagc ggaagtacaa aactttctct gggctgatgt
    59521 ggtgatctgg cagatgccag gctggtggat gggcgcgccg tggacagtga aaaaatatat
    59581 tgatgatgta ttcaccgaag gtcacgggac tctgtatgcc agcgatggtc gtacccgcaa
    59641 agatccgtcg aaaaaatacg gttccggcag cctggtacag gacaaaaaat atatgctttc
    59701 tctaacctgg aacgcaccaa tggaagcctt caccgaaaaa gatcagttct tccacggcgt
    59761 tggcgttgac ggtgtgtatc tgccgttcca taaagcaaac caattcctcg gtatggaacc
    59821 gctgccgaca tttatcgcta atgacgtgat aaaaatgcct gatgttcccc gctatactga
    59881 agaatatcgc aagcatcttg tggaaatttt tggttaacta gagctcaggc tttagaagga
    59941 gttaaccatg cttaccgtaa tcgcagaaat ccgtactcgt cctggtcaac atcaccgtca
    60001 ggcggtattg gatcagtttg ctaaaatcgt tccaaccgta ctgaaagaag aaggttgcca
    60061 cggctatgcg ccaatggtgg attgcgctgc tggcgtgagt ttccagtcta tggcaccgga
    60121 ttctatcgtg atgattgagc agtgggaaag catcgcgcac cttgaagcgc atctgcaaac
    60181 cccgcacatg aaggcgtaca gcgaagccgt aaaaggcgac gtgctggaga tgaatatccg
    60241 tattctgcag ccagggattt aatcctgcct tgtttgcccg gccatcctga ccgggcaatg
    60301 ttctttcctt taaacctcaa tctccgccat gtcgcctttc tcttgcaacc agttgcggcg
    60361 atcttccgag cgtttcttcg ccagcagcat atccatcatc gcgtcagtac gctgatcgtc
    60421 ttcatcatcg atagtcaact gcaccagacg gcgagtgttc ggatcaagcg tggtttcacg
    60481 caattgcatc gggttcattt cccccagacc tttaaaacgc tggacgttcg gcttgccttt
    60541 cttgcgtttt aattgctcaa gtacgccctc tttctcttct tccgtcagcg cgtaataaac
    60601 ctctttcccg agatcaatac ggtagagcgg tggcagtgcg acgtaaacgt gaccgtgttt
    60661 caccaacgcg cggaagtgtt ttacgaacaa agcgcagagc agcgtggcaa tgtgcagacc
    60721 atcagagtcc gcatccgcga ggatacagat tttgccataa cgaagctggc tcagatcgtc
    60781 gctgtcagga tcgataccga tcgctaccga aatatcatgc acttcctgcg aggccagcac
    60841 ttcgtcggaa gaaacttccc aggtgttaag gatcttacct ttcagcggca tgatcgcctg
    60901 atattcacga tcgcgcgcct gcttggcaga tccgcctgcg gagtcacctt ccacgaggaa
    60961 cagttcggta cggttaaggc cctgcgcggt acaatcagcc agtttgccag gcagcgccgg
    61021 gccgctggtc agctttttac gcaccacttt tttagccgca cgcatacgac gctgggcgct
    61081 ggaaatcgcc atctctgcca gcaactccgc cgcctgaacg ttctggttca gccacaggat
    61141 gaaggcatct ttcaccacgc ccgaaacgaa tgccgcacac tgacgcgaag agagacgctc
    61201 tttggtctgc ccggcaaact gcggatcctg cattttaacc gacagaacat aggcgcagcg
    61261 atcccagata tcttccgccg acagcttcac gccgcgcggc agaatgttgc ggtattcaca
    61321 gaactcacgc atcgcgtcca acaggccctg acgcagacca ttaacatggg taccgccctg
    61381 catcgttggg ataaggttga cgtagctttc ggtcagcagt tcaccgcctt ccggcagcca
    61441 cagtagcgcc cagtccacag cttcagtatc gccagcgaaa ttaccgataa acggtttttc
    61501 tggcagcgtc ggtaaaccgt ttaccgcttc cgccaggtaa tcattcagac cgtcctgata
    61561 gcaccagcgc tgttcggtgt tgttgatctc atctttaaag gtaatctcaa cgccggggca
    61621 cagtactgct ttggccttca gcacatgcgt caggcgtgaa acagaaaaac gcgggctgtc
    61681 aaagaaggtt tcatccggcc agaagtgcac gctggtaccg gtattgcgtt taccgcaagt
    61741 gccgacaacc tgtaaatcct gcaccttttc gccattttca aaggcgatgt tataaacctg
    61801 accatcgcgg cgcacgttaa cttctacgcg cttcgacaag gcgttaacta ccgaaatccc
    61861 cacgccatgc aggccgccag agaactggta atttttgtta gagaatttac cgcctgcatg
    61921 cagacggcaa agaatcagtt caaccgccgg tactccttct tccgggtgaa tatccaccgg
    61981 catcccgcgc ccatcgtcaa tcacttctaa cgactggtca gcatgtaaga ttacatccac
    62041 gcgttttgcg tgacccgcca gtgcttcatc gacactgtta tcaataactt cttgccccaa
    62101 atggttaggg cgagtggtat cggtatacat ccccggacgg cggcgaaccg gctcaagccc
    62161 ggtgagtacc tcaatggcat cagcgttata agtttgcgtc atggtttaag ttagcaattc
    62221 gagttgatcg tcagagatgg tgcagaccaa gaaaatcgac gatcgggttg aaataatctt
    62281 cgaagcccgt gaatgcgtgg ttgccgcctt ctatgacagt ctggcggcag gaagcatagt
    62341 acgccaccgc ctggcggtaa tccagcactt catctcccgt ctgttgcagc agccagatca
    62401 aatccggcgc ttccagcggg tcaatctgca tgactttgag atcgtaaata tggcgtgact
    62461 ctagcacata ttgctgcccg gtgtaggggt tctcgttctg accgagatag tccgtcagca
    62521 attcaaacgg gcgtaccgcc gggtttacca ccactgcggg cagcataaaa cattgcgaca
    62581 accaggtggc gtaatatccc cccagtgacg aaccgacaat acccagcgaa tcgccgccat
    62641 gttccaggac aatggattcc agcagttctg ccgcgtcgga aggatacggc ggcaactgcg
    62701 gaatgatcat ctcaacgtca gggtgatgtt ccgccagcca gttttttaac aagctcgctt
    62761 ttgcagagcg cggcgagctg ttgaaaccgt gtaaataaag aagcgtagac atcagtagcc
    62821 ttctgaagcg gtatcaggtt ggaaacgtgt gtccgccagg cgatgcacct cggtggtcag
    62881 cgtgccatca gcatgtaact cgagagtacg ccagccgggc gcgatagtat ccagcgtaaa
    62941 gttggaacag tgcggcttaa actgcacaca ggtcgacggc gttgccagca ggcggcgacc
    63001 gttccagtcg agatccagct cctgatgaat atgaccgcac agcaagtatt tgacgtgcgg
    63061 aaacttcgcc agcacggtat ccagttcgcc cgcgttacgc agactgtgtt gatcgagcca
    63121 actacaaccc gcaggtagcg gatgatgatg cagcagcagc aacgtatggc gttctggcgc
    63181 atcggccagt ttacgttcca gccactcaag ctgaaactcg ctcagctcac cgtgcggcac
    63241 gccaaacacc tggctatcca gcaacaggat ttgccattgc tcaccaataa acacccgctt
    63301 cgccggggag atacccgcat cctgtaacgc gctgtacatc gcgggctgga aatcgtggtt
    63361 gcccggcagc cagacgcagg gcgcacgaaa acttgcgatg ccttcagcga aatgctgata
    63421 ggccgcagag gattgatcct gcgctaaatc acctgtcgcg acaatcaggt cgaattcgtg
    63481 ctggtgtgga cgaatcgcct ccagcaccgc ctggtagctt tcccaggtgt ttacccctaa
    63541 cagggcttcg tgcttttgtg caaacaggtg agtatcggta atttgtaaaa tccttcctct
    63601 ggcctcacca gccagaggaa gggttaacag gctttccaaa tggtgtcctt aggtttcacg
    63661 acgctagtaa accggaatcg ccatcgctcc atgtgctaaa cagtatcgca accagtccgc
    63721 taaaaactgg ttaatttgat gcttttcgtc gcgttgatgc aactttttat taggataatc
    63781 ataccgcgct ttgaagcgaa aaatctgctg gcttgaacac acttcagcca ccatcgcgtc
    63841 atgatacaga cgcaccgtca ttgacggaag gctccagtaa ctgatcgcgg gcgcagtctg
    63901 ttctattgtc accagggtag tgtatcgggt cgattccaca atcgtcagcc gatattgtgc
    63961 gtttgccacc tgatagctta cagtttcgcc gggtgcgtca ttgcgcggta acaaacggcg
    64021 caattgtgaa aagttcatct cgcacaggcg catcatttca ggaaagtcag gtgtgtaacg
    64081 cttcatttat gcccactcat tttttaacgc ttgatgatgc agctgcagcc attgcaaagc
    64141 gatgaccgac gctgcgttgt cgattttccc ctcttctacc cactggtatg cctgttcccg
    64201 gcttaccaca tgaacgcgaa tatcttcgtt ttcatcagcc agaccatgaa taccgcttgc
    64261 ggtcgtggcg tccacttcgc ccaccataat tgacgaacgc tcactggtgc cccccgggct
    64321 tgccaggaaa cttaacaccg gtttggtccg tttgactatc agtcccgcct cttcaatcgc
    64381 ttcgcgacgg gcaacatctt ccacactttc accctcttca atcatcccgg caaccatctc
    64441 cagtagccag ggggtttcgc tggtgtcgta cgcggcaatc cgaatctgct caatcagcac
    64501 aacttcatca cgcactgggt caaagggtag caagactgcg gcgtgaccgc gctcaaaaat
    64561 ttcccgccgt acctcatgac tcatttgccc gttgaataga cgatgacgaa atctataaag
    64621 atctaatgaa aaaaagccgc gataaagtgt ttctcgtgca ataatttcta catcgttttt
    64681 gccaaatgta acgggcaggt tgtctggctt aagcattgtt aatgtcctag cactaatagt
    64741 gaattaaatg tgaatttcag cgacgtttga ctgccgtttg agcagtcata tgttaaattg
    64801 aggcacatta acgccctatg gcacgtaacg ccaacctttt gcggtagcgg cttctgctag
    64861 aatccgcaat aattttacag tttgatcgcg ctaaatactg cttcaccaca aggaatgcaa
    64921 atgaagaaat tgctccccat tcttatcggc ctgagccttt ctgggttcag ttcgttgagc
    64981 caggccgaga acctgatgca agtttatcag caagcacgcc ttagtaaccc ggaattgcgt
    65041 aagtctgccg ccgatcgtga tgctgccttt gaaaaaatta atgaagcgcg cagtccatta
    65101 ctgccacagc taggtttagg tgcagattac acctatagca acggctaccg cgacgcgaac
    65161 ggcatcaact cgaacgcgac cagtgcgtcc ctgcagttaa ctcaatccat ttttgatatg
    65221 tcgaaatggc gtgcgttaac gctgcaggaa aaagcagcag ggattcagga cgtcacatat
    65281 cagaccgatc agcaaacctt gatcctcaac accgcgaccg cttatttcaa cgtgttgaat
    65341 gctattgacg ttctttccta tacacaggca caaaaagaag cgatctaccg tcaattagat
    65401 caaaccaccc aacgttttaa cgtgggcctg gtagcgatca ccgacgtgca gaacgcccgc
    65461 gcgcagtacg ataccgtgct ggcgaacgaa gtgaccgcac gtaataacct tgataacgcg
    65521 gtagagcagc tgcgccagat caccggtaac tactatccgg aactggcggc gctgaatgtc
    65581 gaaaacttta aaaccgacaa accacagccg gttaacgcgc tgctgaaaga agccgaaaaa
    65641 cgcaacctgt cgctgttaca ggcacgcttg agccaggacc tggagcgcga gcaaattcgc
    65701 caggcgcagg atggtcactt accgactctg gatttaacgg cttctaccgg gatttctgac
    65761 acctcttata gcggttcgaa aacccgtggt gccgctggta cccagtatga cgatagcaat
    65821 atgggccaga acaaagttgg cctgagcttc tcgctgccga tttatcaggg cggaatggtt
    65881 aactcgcagg tgaaacaggc acagtacaac tttgttggtg ccagtgagca actggaaagc
    65941 gcacatcgta gcgtcgtgca aaccgtacgt tcctccttca acaacattaa tgcttctatc
    66001 agtagtatta acgcctacaa acaagccgta gtttccgcgc aaagctcatt agacgcgatg
    66061 gaagcgggct actcggtcgg tacgcgtacc attgttgatg tgttggatgc gaccaccacg
    66121 ctgtacaacg ccaagcaaga gctggcgaat gcgcgttata actacctgat taatcagctg
    66181 aatattaagt cagccctggg tacgttgaac gagcaggatt tgctggcact gaacaatgcg
    66241 ctgagcaaac cggtttccac taatccggaa aacgttgccc cgcaaacgcc ggaacagaat
    66301 gctattgctg atggttatgc gcctgatagc ccggcacccg tcgttcagca aacatccgca
    66361 cgcactacca ccagtaacgg tcataaccct ttccgtaact gatgacgacg acggggcttc
    66421 ggccccgtct gaacgtaagg caacgtaaag atacgggtta tctgccgcat tcttccccct
    66481 tctcgcttca atttcgacca gccatcctct attctgatgg gtatttacca ctggtcccgg
    66541 aagacaaaaa tgaaacggac aaaatccata cgccacgcat cgttccgcaa aaactggagc
    66601 gcacgccatc tgacaccagt cgctctcgcg gttgccactg tttttatgct ggctggctgt
    66661 gaaaagagtg atgaaacagt gtctctctat caaaatgctg acgactgttc agctgcaaac
    66721 ccaggcaaaa gcgccgaatg taccaccgcg tacaacaatg cgctgaaaga agccgaacgt
    66781 actgcgccga aatacgccac ccgtgaagac tgtgttgctg aatttggtga aggtcagtgt
    66841 cagcaagcac cagcccaggc tggcatggca ccagaaaacc aggcgcaggc tcagcaatcc
    66901 agcgggagtt tctggatgcc gctgatggcc ggttacatga tggggcgtct gatgggcggc
    66961 ggcgcgggat ttgcacagca gccgctgttc tcctcgaaaa acccggccag tccggcttac
    67021 ggtaaatata ccgacgcgac gggtaaaaac tatggcgcag cccagccagg ccgcaccatg
    67081 accgtaccga agacggcaat ggcaccaaaa ccggcgacca ccactaccgt tacccgtggc
    67141 ggttttggtg aatctgttgc caaacaaagc actatgcagc gtagcgcaac cggcacctct
    67201 tctcgttcaa tgggtggctg ataccgatgg aaagagtcag tattaccgag cgcccggact
    67261 ggcgtgagaa agcccacgaa tacggtttca attttcacac catgtacggc gagccgtact
    67321 ggtgtgaaga tgcttactac aagttgaccc tcgcccaggt tgaaaagctg gaagaagtca
    67381 ccgccgaact gcaccagatg tgcctgaaag tggtggaaaa agtgatcgcc agcgatgagc
    67441 tgatgaccaa attccgcata ccaaaacaca cctggagttt tgtgcgtcaa tcatggctga
    67501 ctcaccagcc atcgctttat tcgcgtcttg atctggcgtg ggatggcact ggcgaaccta
    67561 aacttctgga aaataacgcc gatacgccaa cgtcactata cgaggcggcg ttctttcagt
    67621 ggatctggct ggaagatcag cttaacgccg gtaacttgcc ggagggcagc gaccagttta
    67681 acagtctgca agaaaaactg atcgatcgct tcgttgagct gcgtgaacag tatggcttcc
    67741 agttgctgca tctcacctgc tgtcgcgaca cggtggaaga tcgcggaacc attcagtatt
    67801 tgcaggactg cgcaaccgaa gctgaaatcg ccaccgagtt cctctacatc gacgagatag
    67861 gtttaggcga aaaaggccag ttcacggatt tacaggatca ggtgatttct aacctgttca
    67921 agctttatcc gtgggaattt atgctgcgcg aaatgttctc caccaagctg gaagatgctg
    67981 gcgtacgctg gctggaaccg gcgtggaaga gcattatctc caacaaggca cttctaccgc
    68041 tactgtggga gatgttcccg aatcacccga acctgctgcc cgcttatttt gcggaagatg
    68101 atcatccgca aatggaaaaa tatgtggtta aaccgatctt ctcccgtgaa ggcgcaaacg
    68161 tgtcgatcat tgagaacggc aaaaccattg aagcagcgga aggtccgtat ggcgaagaag
    68221 ggatgattgt tcagcaattc cacccgttac cgaaattcgg cgacagctat atgctgattg
    68281 gtagctggct ggtgaacgat caacctgccg gaattggcat tcgtgaagac cgtgcgttga
    68341 tcacccagga tatgtctcgg ttttatccac atatttttgt tgaataagcc acgataccgg
    68401 atggcactcg ccatccggta attgttagcc tatctgcacc gacagcatac ttaaactgcc
    68461 catttctata ccatcgaccg gaatggtaat tggctcctgc ccatcccaca cacctaacac
    68521 atacaacaac ggcagatagt gctctggcgt tgggttcaat aacgcgccac cttcatggtc
    68581 gagataattc accagaggat gctgctctac cggcccttgc cacgtcagat tcgctttcac
    68641 atactcatta aacgacgtcg cccacggata cggtgaacta tcaccgtgcc acttcactgt
    68701 gcgcaggtta tgcaccacgt taccgctggc gaccaacatt attccttcat cacgcagcgc
    68761 cgcgagtttg cgccccattt cgaaatgcca ggcggcaggt ttgctactgt cgatactcaa
    68821 ctgcaccata gggatatcgg cgtcaggata catcttaatc agcacgcccc acgagccgtg
    68881 gtcaaagccc caggcttctt tatccagcgt caccgggatc ggcgctaaca gctcaaccag
    68941 acgctgtgcc agcgcaggcg aacccggagc aggataatgc gtatcgtaca gcgcctgcgg
    69001 gaagccacca aagtcatgaa tcgtgggcgg cgtctccatc gcggtcactc ctgttccacg
    69061 ggtaaaccag tgagccgaaa ccaccacaat cgcttgcggg cgtggcaatg tcatccccaa
    69121 cttctgccag ctgcgggtat acaaattatc ttccagcacg ttcatcggac taccgtgacc
    69181 taaaaacaat gctggcatac gtgttgaaga catgatgata tccttaacta aaggtgtcat
    69241 tttgatatcc tcacaatacg cttgttcggc ggagtaagaa cccggataac aatgatgatg
    69301 atcatcagtt attttgacga tctgcctgaa ggtgaagatt tataaggagt tgtcgatgtc
    69361 agtacctctc attctgacca tactggcggg ggcagccacg tttattggcg cgtttctcgg
    69421 cgttctcgga caaaaaccct cgaaccgctt actggcgttt tcgctaggtt ttgcggcggg
    69481 gatcatgttg ctcatctcat taatggaaat gcttcctgcc gcactagcag ctgaaggaat
    69541 gtcgccggtg ttgggttatg gaatgtttat cttcggtctg cttggctact ttggcctgga
    69601 ccgcatgttg ccgcatgcgc atccgcagga tttaatgcaa aaatcggtgc agctgttgcc
    69661 aaaatcgatc aagcgcacag ccattctgct cactctcggc atcagtctgc ataacttccc
    69721 ggaagggatt gccacctttg tcacggcgag cagcaacctg gagctgggat ttggcatcgc
    69781 actggccgtc gcgttgcaca atatccctga aggtctggca gtggcaggcc cggtttatgc
    69841 ggcaacgggg tctaaacgca ccgcgattct gtgggcgggg atttctggac tagcagaaat
    69901 tcttggtggt gtgctggcgt ggttaatcct cggtagcatg atttccccgg tggtcatggc
    69961 ggcaatcatg gcggcggttg caggaattat ggtggcgctc tcggttgatg aattaatgcc
    70021 gctcgccaaa gagattgacc ctaataataa ccccagctat ggcgtactat gtggaatgtc
    70081 agtgatggga ttcagtttag tgctgctaca aacggcggga attggttaaa aacaacatca
    70141 gaagttgtcc agcacatgga caacttctgc cagcttcacg aaagccttat tctacctttt
    70201 cggctgtctc ttctctcgta ctgtttaagt atttgttccg cgcttcaagc agtgcgtctc
    70261 tgtcctggcg aaacgcccga caataacgtt tcaaatgcag gaaataaccc accacaacca
    70321 gagcaatcaa tacaatccag taccaggcga taaacatcct tttaccctta atatttaaca
    70381 atactaaaca tttagcgcat aaatttcaca tatccttttt cggatatatt catatggtcg
    70441 cataataacg gaacaattat atgattttga tcataatgaa aaagaatgcc tgatattaat
    70501 gaaattttct catagaaaga gaagcggaat taacatcttg catagcaggg caaaataaaa
    70561 accgaagcca gctacaactt cggtttcttg attaaggcag taattaagca gcggttttca
    70621 gctggcttta cgctcatgtg cctgacggta tgccaccagg tcttcaatag tcacgagcgc
    70681 catattgtgt ttattggcaa actcaatgca ctctggtgca cgcgccatcg tgccatcgtc
    70741 attagtcagc tcacacagca caccagccgg tttaaagcct gccagcgtca tcaaatcaat
    70801 agtcgcttca gtatgaccgc cacgcgtcag tacaccacct gcctgcgcgc gaagtgggaa
    70861 aacgtggccc gggcgattca gatcagaagg ttttgcgcca tcggcaatcg ctgcgcgaac
    70921 ggtcgtaata cgatcagcgg cagaaacacc ggtagtcaca ccttcagccg cttcaatggt
    70981 cacggtaaaa ccggtgccat aggcgctggt gttattttct accatcattg gcagatcaag
    71041 ttgtttacgg cgatcttcag tgatgcacag gcaaacaata ccgctaccgt ggcgaatggt
    71101 cagcgccatc tgctcaacgg tcatggtttc tgccgggaag atcatatcgc cttcgttttc
    71161 acggtcttca tcatcaagca ccattacacc gcgtccttca cgcagcgcag ccagtgcatt
    71221 ttcaacacgt tcgaaaggcg taccaaaaga ggaaagtagc gtctgattca tggtaaaaaa
    71281 acctcactaa aattatggtt accagaatca gggcagtctt aggagtggcg gcatatagcc
    71341 aaaataacgt gagcgggtcc atgcccgaca gaatcgttac tctctcccat ccggactcta
    71401 accgtcggcc ccggaattac accggatctg ctgtcctttg agttcgcacc caaagcgctc
    71461 gcgggctttc aactgagttg atttaccgcc ggtggggaat ttcgccccgc cctgagaata
    71521 agcggattca ctataacgct aatgattagc ggcagcaacg catagcttca cataattctg
    71581 gtttatgact tacccttatc gcactacaat ggtactcaac acctatcagt acagggaaac
    71641 tacaatgatt gacccgaaaa aaattgagca aatcgctcgc caggttcacg aatcaatgcc
    71701 taaaggaatc agggagttcg gggaagatgt ggagaaaaaa atccgccaaa ccctacaagc
    71761 tcagctgacg cgcctcgacc ttgtaagccg tgaagagttc gacgtccaaa cgcaagtcct
    71821 gttacggact cgtgagaaac tggcgctgct tgaacaaaga atcagcgaac tggaaaatcg
    71881 cagtactgag atcaaaaagc agccagatcc agagactctc cccccaacgc tgtaaacatt
    71941 tgtctttaat gcactgtaaa ttatgaatta atcctctaat ttacagtgtt ttctattaca
    72001 ttatcgctta atcccattca gagtatcatc acaaaaatga atgattgcaa ttgaaaatat
    72061 aattgttatg tattctttaa gttttactta atcgata
//
LOCUS       DAJTRD010000006        70706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTRD010000006 DAJTRD010000000
VERSION     DAJTRD010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.006"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..788
                     /locus_tag="OO315_000457"
     CDS             <1..788
                     /locus_tag="OO315_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5889493.1"
                     /translation="RSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDN
                     NHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALI
                     DANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVV
                     PNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSG
                     ISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            799..1890
                     /locus_tag="OO315_000458"
     CDS             799..1890
                     /locus_tag="OO315_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5889494.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1933..2793)
                     /gene="rsmI"
                     /locus_tag="OO315_000459"
     CDS             complement(1933..2793)
                     /gene="rsmI"
                     /locus_tag="OO315_000459"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5889495.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2858..4894
                     /gene="lpoA"
                     /locus_tag="OO315_000460"
     CDS             2858..4894
                     /gene="lpoA"
                     /locus_tag="OO315_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5889496.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4852..5247
                     /locus_tag="OO315_000461"
     CDS             4852..5247
                     /locus_tag="OO315_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5889497.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5267..5857
                     /gene="diaA"
                     /locus_tag="OO315_000462"
     CDS             5267..5857
                     /gene="diaA"
                     /locus_tag="OO315_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5889498.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5867..6442
                     /gene="yraP"
                     /locus_tag="OO315_000463"
     CDS             5867..6442
                     /gene="yraP"
                     /locus_tag="OO315_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5889499.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6556..7596)
                     /locus_tag="OO315_000464"
     CDS             complement(6556..7596)
                     /locus_tag="OO315_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5889500.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVSGLALLF"
     gene            complement(7669..8304)
                     /locus_tag="OO315_000465"
     CDS             complement(7669..8304)
                     /locus_tag="OO315_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5889501.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8432..8950
                     /gene="yhbO"
                     /locus_tag="OO315_000466"
     CDS             8432..8950
                     /gene="yhbO"
                     /locus_tag="OO315_000466"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5889502.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8930..9373)
                     /locus_tag="OO315_000467"
     CDS             complement(8930..9373)
                     /locus_tag="OO315_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5889503.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9424..9726
                     /gene="yhbQ"
                     /locus_tag="OO315_000468"
     CDS             9424..9726
                     /gene="yhbQ"
                     /locus_tag="OO315_000468"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5889504.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9713..10216)
                     /locus_tag="OO315_000469"
     CDS             complement(9713..10216)
                     /locus_tag="OO315_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5889505.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10210..10734)
                     /locus_tag="OO315_000470"
     CDS             complement(10210..10734)
                     /locus_tag="OO315_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5889506.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10943..11938
                     /locus_tag="OO315_000471"
     CDS             10943..11938
                     /locus_tag="OO315_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5889507.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11947..12825
                     /locus_tag="OO315_000472"
     CDS             11947..12825
                     /locus_tag="OO315_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5889508.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12906..13913
                     /locus_tag="OO315_000473"
     CDS             12906..13913
                     /locus_tag="OO315_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5889509.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14031..15275)
                     /gene="mtr"
                     /locus_tag="OO315_000474"
     CDS             complement(14031..15275)
                     /gene="mtr"
                     /locus_tag="OO315_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5889510.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15429..17318)
                     /gene="deaD"
                     /locus_tag="OO315_000475"
     CDS             complement(15429..17318)
                     /gene="deaD"
                     /locus_tag="OO315_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5889511.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17311..17391)
                     /gene="yrbN"
                     /locus_tag="OO315_000476"
     CDS             complement(17311..17391)
                     /gene="yrbN"
                     /locus_tag="OO315_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5889512.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17498..18382)
                     /gene="nlpI"
                     /locus_tag="OO315_000477"
     CDS             complement(17498..18382)
                     /gene="nlpI"
                     /locus_tag="OO315_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5889513.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18491..20626)
                     /gene="pnp"
                     /locus_tag="OO315_000478"
     CDS             complement(18491..20626)
                     /gene="pnp"
                     /locus_tag="OO315_000478"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5889514.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20873..21142)
                     /gene="rpsO"
                     /locus_tag="OO315_000479"
     CDS             complement(20873..21142)
                     /gene="rpsO"
                     /locus_tag="OO315_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5889515.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21291..22235)
                     /gene="truB"
                     /locus_tag="OO315_000480"
     CDS             complement(21291..22235)
                     /gene="truB"
                     /locus_tag="OO315_000480"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5889516.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22235..22636)
                     /gene="rbfA"
                     /locus_tag="OO315_000481"
     CDS             complement(22235..22636)
                     /gene="rbfA"
                     /locus_tag="OO315_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5889517.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22800..25448)
                     /gene="infB"
                     /locus_tag="OO315_000482"
     CDS             complement(22800..25448)
                     /gene="infB"
                     /locus_tag="OO315_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5889518.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25473..26960)
                     /gene="nusA"
                     /locus_tag="OO315_000483"
     CDS             complement(25473..26960)
                     /gene="nusA"
                     /locus_tag="OO315_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5889519.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26988..27440)
                     /gene="rimP"
                     /locus_tag="OO315_000484"
     CDS             complement(26988..27440)
                     /gene="rimP"
                     /locus_tag="OO315_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5889520.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27647..27723)
                     /locus_tag="OO315_000485"
     tRNA            complement(27647..27723)
                     /locus_tag="OO315_000485"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27687..27689),aa:Met,seq:cat)
     gene            28071..29414
                     /gene="argG"
                     /locus_tag="OO315_000486"
     CDS             28071..29414
                     /gene="argG"
                     /locus_tag="OO315_000486"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5889521.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29422..31029)
                     /locus_tag="OO315_000487"
                     /pseudo
     CDS             complement(29422..31029)
                     /locus_tag="OO315_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31507..31593)
                     /locus_tag="OO315_000488"
     tRNA            complement(31507..31593)
                     /locus_tag="OO315_000488"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31557..31559),aa:Leu,seq:gag)
     gene            complement(31608..31940)
                     /gene="secG"
                     /locus_tag="OO315_000489"
     CDS             complement(31608..31940)
                     /gene="secG"
                     /locus_tag="OO315_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5889522.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32168..33505)
                     /gene="glmM"
                     /locus_tag="OO315_000490"
     CDS             complement(32168..33505)
                     /gene="glmM"
                     /locus_tag="OO315_000490"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5889523.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33498..34346)
                     /gene="folP"
                     /locus_tag="OO315_000491"
     CDS             complement(33498..34346)
                     /gene="folP"
                     /locus_tag="OO315_000491"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5889524.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34436..36370)
                     /gene="ftsH"
                     /locus_tag="OO315_000492"
     CDS             complement(34436..36370)
                     /gene="ftsH"
                     /locus_tag="OO315_000492"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5889525.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36470..37099)
                     /gene="rlmE"
                     /locus_tag="OO315_000493"
     CDS             complement(36470..37099)
                     /gene="rlmE"
                     /locus_tag="OO315_000493"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5889526.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37225..37518
                     /gene="yhbY"
                     /locus_tag="OO315_000494"
     CDS             37225..37518
                     /gene="yhbY"
                     /locus_tag="OO315_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5889527.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37674..38150)
                     /gene="greA"
                     /locus_tag="OO315_000495"
     CDS             complement(37674..38150)
                     /gene="greA"
                     /locus_tag="OO315_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5889528.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38398..39831
                     /gene="dacB"
                     /locus_tag="OO315_000496"
     CDS             38398..39831
                     /gene="dacB"
                     /locus_tag="OO315_000496"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5889529.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40017..41189)
                     /gene="cgtA"
                     /locus_tag="OO315_000497"
     CDS             complement(40017..41189)
                     /gene="cgtA"
                     /locus_tag="OO315_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5889530.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41205..42170)
                     /locus_tag="OO315_000498"
     CDS             complement(41205..42170)
                     /locus_tag="OO315_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5889531.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42297..42554)
                     /gene="rpmA"
                     /locus_tag="OO315_000499"
     CDS             complement(42297..42554)
                     /gene="rpmA"
                     /locus_tag="OO315_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5889532.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42575..42886)
                     /gene="rplU"
                     /locus_tag="OO315_000500"
     CDS             complement(42575..42886)
                     /gene="rplU"
                     /locus_tag="OO315_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5889533.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43144..44115
                     /gene="ispB"
                     /locus_tag="OO315_000501"
     CDS             43144..44115
                     /gene="ispB"
                     /locus_tag="OO315_000501"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5889534.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44342..44620
                     /gene="sfsB"
                     /locus_tag="OO315_000502"
     CDS             44342..44620
                     /gene="sfsB"
                     /locus_tag="OO315_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5889535.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44668..45927)
                     /gene="murA"
                     /locus_tag="OO315_000503"
     CDS             complement(44668..45927)
                     /gene="murA"
                     /locus_tag="OO315_000503"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5889536.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45982..46236)
                     /gene="ibaG"
                     /locus_tag="OO315_000504"
     CDS             complement(45982..46236)
                     /gene="ibaG"
                     /locus_tag="OO315_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5889537.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46396..46689)
                     /gene="mlaB"
                     /locus_tag="OO315_000505"
     CDS             complement(46396..46689)
                     /gene="mlaB"
                     /locus_tag="OO315_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5889538.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46689..47324)
                     /gene="mlaC"
                     /locus_tag="OO315_000506"
     CDS             complement(46689..47324)
                     /gene="mlaC"
                     /locus_tag="OO315_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5889539.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47343..47894)
                     /gene="mlaD"
                     /locus_tag="OO315_000507"
     CDS             complement(47343..47894)
                     /gene="mlaD"
                     /locus_tag="OO315_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5889540.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47899..48681)
                     /gene="mlaE"
                     /locus_tag="OO315_000508"
     CDS             complement(47899..48681)
                     /gene="mlaE"
                     /locus_tag="OO315_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5889541.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48689..49498)
                     /gene="mlaF"
                     /locus_tag="OO315_000509"
     CDS             complement(48689..49498)
                     /gene="mlaF"
                     /locus_tag="OO315_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5889542.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49708..50673
                     /locus_tag="OO315_000510"
     CDS             49708..50673
                     /locus_tag="OO315_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5889543.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50687..51673
                     /gene="kdsD"
                     /locus_tag="OO315_000511"
     CDS             50687..51673
                     /gene="kdsD"
                     /locus_tag="OO315_000511"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5889544.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51694..52260
                     /gene="kdsC"
                     /locus_tag="OO315_000512"
     CDS             51694..52260
                     /gene="kdsC"
                     /locus_tag="OO315_000512"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5889545.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52257..52832
                     /gene="lptC"
                     /locus_tag="OO315_000513"
     CDS             52257..52832
                     /gene="lptC"
                     /locus_tag="OO315_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5889546.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52801..53358
                     /gene="lptA"
                     /locus_tag="OO315_000514"
     CDS             52801..53358
                     /gene="lptA"
                     /locus_tag="OO315_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5889547.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53365..54090
                     /gene="lptB"
                     /locus_tag="OO315_000515"
     CDS             53365..54090
                     /gene="lptB"
                     /locus_tag="OO315_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5889548.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54138..55571
                     /gene="rpoN"
                     /locus_tag="OO315_000516"
     CDS             54138..55571
                     /gene="rpoN"
                     /locus_tag="OO315_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5889549.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55594..55881
                     /gene="hpf"
                     /locus_tag="OO315_000517"
     CDS             55594..55881
                     /gene="hpf"
                     /locus_tag="OO315_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5889550.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            55999..56490
                     /gene="ptsN"
                     /locus_tag="OO315_000518"
     CDS             55999..56490
                     /gene="ptsN"
                     /locus_tag="OO315_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5889551.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56536..57390
                     /gene="rapZ"
                     /locus_tag="OO315_000519"
     CDS             56536..57390
                     /gene="rapZ"
                     /locus_tag="OO315_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5889552.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57387..57659
                     /gene="npr"
                     /locus_tag="OO315_000520"
     CDS             57387..57659
                     /gene="npr"
                     /locus_tag="OO315_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5889553.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57872..58504
                     /gene="yrbL"
                     /locus_tag="OO315_000521"
     CDS             57872..58504
                     /gene="yrbL"
                     /locus_tag="OO315_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5889554.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="OO315_000522"
                     /pseudo
     CDS             complement(58501..59228)
                     /gene="mtgA"
                     /locus_tag="OO315_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="OO315_000523"
     CDS             complement(59225..59878)
                     /gene="elbB"
                     /locus_tag="OO315_000523"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5889555.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="OO315_000524"
     CDS             complement(60108..62444)
                     /gene="arcB"
                     /locus_tag="OO315_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5889556.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62540..63469)
                     /locus_tag="OO315_000525"
     CDS             complement(62540..63469)
                     /locus_tag="OO315_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5889557.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64144..68604
                     /gene="gltB"
                     /locus_tag="OO315_000526"
     CDS             64144..68604
                     /gene="gltB"
                     /locus_tag="OO315_000526"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5889558.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68617..70035
                     /gene="gltD"
                     /locus_tag="OO315_000527"
     CDS             68617..70035
                     /gene="gltD"
                     /locus_tag="OO315_000527"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5889559.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70219..70698
                     /locus_tag="OO315_000528"
     CDS             70219..70698
                     /locus_tag="OO315_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5889560.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17314 a        19173 c        17558 g        16661 t
ORIGIN      
        1 cgcgtagcct ggtttcctat cagatgacca gccagaaaga tcgcccaacc caacatgaaa
       61 tgcgtcttga tggctcactg ctggatgatg gtcgcctgag ctatagcctg gaacaaagtc
      121 tggatgacga taacaaccat aacagtagcc tgaacgccag ttaccgttca ccttatggaa
      181 ccttcagtgc cggatacagt tacggtaatg acagcagcca atacaattac ggcgttaccg
      241 gcggcgtggt tatccatcct catggcgtga cgctctcgca atatctgggc aacgcttttg
      301 cgcttatcga tgctaacggg gcttctggcg tgaggataca aaactatccg gggattgcta
      361 ctgatccctt tggctatgca gtggttcctt atctcacgac ttatcaggaa aaccgtctct
      421 cggtagatac tacgcagctg cccgataacg tcgatcttga acaaacaaca cagtttgtgg
      481 tgcccaacag aggtgcaatg gtagcggcgc gtttcaacgc caatatcggt tatcgcgtac
      541 ttgttacagt cagcgatcgc aacggtaaac cgttgccctt tggcgctctt gccagcaacg
      601 atgatacggg gcaacaaagt atcgtcgatg agggcggcat actatatctc tctgggatat
      661 cgagtaaatc acaaagctgg actgtacgct ggggaaatca ggcagatcaa caatgtcagt
      721 ttgcttttag tacaccggat tcagaaccaa caacctctgt attacaaggc acagcgcagt
      781 gccattaagg ataaaaaaat gaaaagagcg cctcttataa caggactttt gttgatatcc
      841 acatcctgcg cttatgcctc ctcaggaggg tgtggagccg acagcactag cggtgcgaca
      901 aattacagca gtgtggttga tgatgttacg gtgaaccaga cagataacgt gacaggacgg
      961 gagtttacct ctgcaacgct aagtagcact aactggcaat acgcctgttc ctgctctgcg
     1021 ggtaaggcag ttaaacttgt ctatatggtc agccccgtac ttaccaccac tggacatcag
     1081 acaggatatt acaaactcaa tgacagcctg gatattaaaa ccacattaca ggcaaacgac
     1141 attccaggac tcacaaccga ccaggttgtc tctgttaaca cccgattcac acagataaaa
     1201 agcagcaccg tatattctgc tgcaacccaa acgggtgttt gccagggtga cacgtctcgt
     1261 tatggacccg ttaatattgg tgcaaatacc acctttaccc tgtatgtcac caagccattt
     1321 ctcggctcga tgaccattcc gaaaacggat attgccgtca ttaaaggcgc gtgggtcgat
     1381 ggaatgggaa gcccgtctac aggtgacttc catgatttag tcaagttatc gattcaggga
     1441 aatctcaccg ccccacagtc gtgcaaaatt aatcagggcg atgttattaa agttaatttt
     1501 ggattcatca atggtcagaa gtttaccacc cgcaatgcca tgccagacgg ttttactcca
     1561 gtagactttg atatcactta tgactgtggt gatacttcaa agattaaaaa ctcgttgcaa
     1621 atgcgcatcg acggtacaac tggggtagta gaccagtaca acctggtcgc caggcgaaga
     1681 agttcagaca atgtgcccga tgtcggtatt cgtattgaaa atctcggcgg cggagttgca
     1741 aatattcctt ttcagaacgg tatccttccc gttgatcctt ccgggcatgg caccgtcaat
     1801 atgcgcgcct ggccagttaa tctggtcggt ggtgagctgg aaacaggaaa atttcagggc
     1861 acagccacca ttaccgtcat ggtgcggtaa aagaagacgt tgcgttttga ttaacgcaac
     1921 gcttcggcac tgttacccct gctgctccag cgcatactta tacagcgcat ttttcttcac
     1981 gccatgaatt tctgcggcca gcgccgccgc ttttttcagc ggcagttctg cctgtagcag
     2041 cgccagcgtg cgcagggcat cggcgggtaa gtcttcttcc tgtgctttat gaccttcgac
     2101 aatcagcacc atttcgcctt tgcgacggtt ttcatcttcc tttacccacg ccagcagctc
     2161 gccaacgggc gcgccgtgaa tggtttccca ggttttggtc agctcacgcg ccagaaccac
     2221 gtagcgggat tcgcctaata ccgcaacgat atcttccagg ctatctaaca gacggtgggt
     2281 agattcataa aaaatcagcg tgcgcggctc cgcttcaatg gcttttagcg catcacggcg
     2341 gccttttgat ttggcaggta aaaagccttc gtaacagaaa cggtcagatg gtaaacccgc
     2401 ggcgcttaac gcagtgatag cagcacacgg tccgggtagc ggcaccacac gaatccccgc
     2461 ttcacggcag gtacgcacca gatggtagcc aggatcgtta attagcggcg ttccggcatc
     2521 ggaaaccagc gcaatgtttt gcccctcttg cagctttgcc agcagcgttt cggctttttg
     2581 ttgttcgtta tggtcgtgca gcgcaaacaa ccgggcatta atcccaaaat gttgcagcaa
     2641 taaaccggtg tgacgagtat cctcggcggc aatcagatca acggcctgta atacctctaa
     2701 cgtacgctgg gtgatatccg ccagattgcc gattggcgtc ggtacaatgt aaagctggcc
     2761 ctgagaatta tccgccgatt ggtgttgttt cattgtgtcg tccgtattgc cgatttaata
     2821 ttgagcattg cgtaaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg
     2881 tttgaaagcc gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg
     2941 cacccatact cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc
     3001 cttttatctt cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact
     3061 cgccattcgt gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca
     3121 actaccgcaa gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggcagagat
     3181 taaactggcg cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc
     3241 cgatttagaa caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg
     3301 gcgtccttcc attgatttac tgcgcgcgtt aattgctcag gaaccgctgc ttggcgcgaa
     3361 agaaaaacag cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca
     3421 ggcgaatacg ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca
     3481 gcgcgtctgg tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg
     3541 gcagaaacgt tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt
     3601 aaaagcgttt aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca
     3661 ggcagcggta tttggtcgca ctattcagca aggctttgaa gccgcgaaaa atatcggcac
     3721 tcagccagtg gcggctcagg tagctgccgc acctgccgca gacgtagcag aacaacctca
     3781 gccgcaaact gcggatagcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg
     3841 tgatcagcct gcggcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt
     3901 aagcgcaccc gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact
     3961 tagccagatc ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct
     4021 gaaaaataac gttgaagagt tgctgaagag caacaccccg ctgaacgtac tggcactgaa
     4081 ccagccggag aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt caccggaaga
     4141 cgaagcgcgc gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct
     4201 gatcccacgc agtgcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa
     4261 actgggcggc ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg
     4321 tgttaacggc ggttctggta ttgctttaac gggtagcccg attactccca gagcgacaac
     4381 cgactccggc atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt
     4441 caccaataat ggcggtcgtg tcgatgcggt ctacattgtg gcaacgccgg gtgaaatcgc
     4501 ttttatcaaa ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa cgctgtacgc
     4561 cagttcccgc agcgcacaag gcaccgcagg cccggatttc cgactggaga tggaaggctt
     4621 gcagtacagc gaaatcccga tgctggcagg cggtaatctg ccgttaatgc agcaggcgct
     4681 cagcgcggtg aacaacgatt attcgctggc tcgcatgtat gcgatgggcg ttgatgcctg
     4741 gtcgttggca aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa
     4801 taccggaagt ctgacggcta acccggattg cgtgattaac aggaagttat catggctaca
     4861 gtaccaacaa ggtcaggtag tccccgccag ttaaccacca aacagaccgg cgatgcgtgg
     4921 gaagcacaag cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg
     4981 aacgagcgtg gcggcgagat cgatctgata atgcgtgaag gcctgaccac cgtttttgtc
     5041 gaggtacgct atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc
     5101 aaacaacaca aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt
     5161 gatactgtgg attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt
     5221 aaggatgcct ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat
     5281 taaagcttgc ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga
     5341 tgccatctcc cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct
     5401 ctgttgtggt aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa
     5461 ccgtttcgaa acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt
     5521 cttaacggcg attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc
     5581 gctgggtcat gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat
     5641 tgttaaagca gttgaagccg ccgttacgcg tgatatgacc attgtggcat tgaccggcta
     5701 tgacggcggc gaacttgcag gtttgttagg gccacaggat gtggagatcc gcattccttc
     5761 gcatcgtagt gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct
     5821 gatcgataac acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc
     5881 gccaatcgca gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt
     5941 gggtaccgct gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt
     6001 ggacgatggt accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa
     6061 gaaagaaacc cgtattaatg tcacggccta tcagggcaaa gtgctgctgg ttgggcagtc
     6121 accaaatgct gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa
     6181 cgaagtgtat aacgagattc gtcagggcca gccgattggt ctgggcgaag catctaacga
     6241 tacgtggatc accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc
     6301 caacgtgaaa gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg
     6361 tgaagcgaaa gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggttaccac
     6421 agcgtttacg tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc
     6481 ttatcaggcc tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac
     6541 atgttttccc tcaaatcaga acaacagcgc cagcccgctg acaatcacac cagacactgc
     6601 caccatcgcc cctgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag
     6661 tgacggcaaa ctcactgccg ggagcgtcat caacaatgcc agcgccggag cggttcccat
     6721 acctgccagc atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa
     6781 gcatcctgct accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc
     6841 atgggggaat aaccagacgc gtgcggcacc caacaccagt actgcaagga tgtaaaccgg
     6901 gatcgtactc cagaaaagtg tccatagcgc cctgccccag cggctaaaaa aaccgctctg
     6961 tgcttccggt atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt
     7021 ttgcaccagc gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc
     7081 aaaaccccag ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt
     7141 gcccatccag aatgccagcg caccgcccat cgatacctgt tggcgacgca ttcccgccgc
     7201 gaccggagcc gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag
     7261 cgtgccgcga aagcgcgatt gcccaagcgt acgcaacaac cagtcacgcg ggatcagcac
     7321 ctgaatcaac gaaccgagga tcacccccag caccgccgct ttccatactg cgaggaaata
     7381 gatcatcgcg taatccaacg ccgcttgcca cggattagca tccgcctgcg caaggataga
     7441 tttaccgata ctgtgggttt cggctgcagt aaacgctttg ccgtagtaag gttgccattt
     7501 cacataccag agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat
     7561 gggcgttgcc gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg
     7621 ataattgagc gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct
     7681 tttgcgtaat tctgaagagc ttaaaatcgt cacgccttca tgctcaggcc gcatcgcctc
     7741 tgccagcatg acccgcgcca cgtctctcgc atcaatggat ttccagttac ctggtaacaa
     7801 acggaacagc ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagtaa
     7861 catcgacggg cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat
     7921 ctcccctttg acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac
     7981 caccagcata tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt
     8041 gtaatcggca tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca
     8101 acaaaacaca atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca gttgcggatc
     8161 atggggatta aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cagcaatggc
     8221 gttaactttc ggttcattaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc
     8281 gcctgtaatc agtacctgac tcatcttcgt tcctttacag aattgtctgc cttgcgctcc
     8341 ccggctcatg caccagtctt aatagaccat gaggtaatta tccccgattg tggaaaattc
     8401 gcctcatcca atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcac
     8461 tgatgaattt gaagattcag aatttacttc acccgcagac gagttccgta aagccggaca
     8521 cgaagtgatt actattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc
     8581 cagcgtgacc atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgtt
     8641 gctaccgggt ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac
     8701 ccgtgatttt gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct
     8761 gatcagcgcc gatgtgattc gggggcgcaa actgaccgca gttaaaccga tcattattga
     8821 tgttaaaaat gcgggcgtgg aattttacga tcaggaagtc gtggttgata aagatcagct
     8881 ggttaccagc cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct
     8941 cggtgcctga gtcgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga
     9001 atttgatttc atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa
     9061 agcgacgatt gtacgccttg cgcgcgaggt cgctttcctc accttccagc ctgcggatct
     9121 cacctttaaa ctgcacaccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc
     9181 cggcaaccgc agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa
     9241 tgtagaaggc gactttctgc gcatcaaaaa gataaaaggc attagcgcac cagagttccc
     9301 cttcctgctg cacacaccag gtgacaacat gttgttttgc cagccaacgg ctgatggcga
     9361 tgagtgtttc cattgctgtt ctcttttata ctgtgggcct gaccttaaca tgcgatgcag
     9421 acgatgacac cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg
     9481 atcaccacgg atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca
     9541 ctgcgcggga aaggagaact gacgctggcg ttttctgcgc cagtgggcga ccgttcgctg
     9601 gcactacggg cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta
     9661 gcggaaggcg cagggtttgc agaactgttg agcagcctgc aaaccccgga gattaaaagc
     9721 gattgaaatg ctcgtgatac tcaaccagac cggttacgcc attcagcgcg tcatccgcta
     9781 aacgatgtac caggaaggcg ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg
     9841 ccgccagttc aaagccgaaa cgactgtaca gcgccggatc gcccagcgtc accactgcgg
     9901 catagccgaa ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct
     9961 gtccacggta tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc
    10021 cctgcacatc aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg
    10081 ccaccagccc cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg
    10141 catcactttc gaatgagcga cgcagcaggg catcaatacc cggcgcatca atgggaattt
    10201 ctactcgaat tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat
    10261 tcccgcctca acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg
    10321 ctccagttca atggcgtcca tcaggttttt cacatacacc cccagctccg tatcgccttc
    10381 aatcaccagc cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat
    10441 cagcagatcg ctggcgtcgg cactaaaact cacatcagct tgcgtgttct ggctgaccac
    10501 cagtttgcca ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact
    10561 taaccagcgg ccttcaagaa actccagctc gccatcatcc agcgcctgac ggaattgcca
    10621 gcttaagacc tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac
    10681 actcaacaga gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt
    10741 tactccctgt ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata
    10801 aatcaacaat tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc
    10861 aaaaattgtc gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgat
    10921 agcgcaacct tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct
    10981 taaggcggcc atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgataccaa
    11041 tgcccgtcac ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt
    11101 tgtccatcaa catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg
    11161 ttacgcccgt tggcagcgtg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat
    11221 cctcgccgac ctcgccatgc tggagtacgc cgccgagcgt tacccgcata ttgaacgtca
    11281 tgtgtcagtg caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt
    11341 tgacgttgcc cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacagctggc
    11401 acgggttact cctgtcccgc tggaagtgtt tgctttcggc agcctgtgta ttatgtcgga
    11461 aggccgttgc tatctgtcgt cgtatctgac gggtgagtcg cccaacactg tgggtgcgtg
    11521 ttctccggcc cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa
    11581 cgaagtgctg atcgaccgtt atcaggacgg cgaaaacgca ggttatccga cgctgtgtaa
    11641 agggcgttat ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa
    11701 taccctggaa ctgctgccgg agttaatggc tgcgaatatt gcttcggtga aaattgaagg
    11761 ccgccagcgt agcccggcgt atgtcagcca ggcggcgaaa gtctggcgtc aggctatcga
    11821 ccgttgtaag gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg
    11881 gtcgatgtcc gaaggcacgc aaaccaccct tggcgcgtat caccgtaaat ggcagtgaga
    11941 agagcaatga aatattcctt agggccagtg ctgtggtact ggccaaaaga gacgctggaa
    12001 gatttttatc agcaagccgc cgccagcagc gccgacgtga tttatcttgg tgaagcggta
    12061 tgcagcaagc gccgggcaac caaagttggc gactgtctgg agatggcaaa atcgctcgcc
    12121 ggaagtggca agcagattgt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg
    12181 ggcgaactga aacgctatgt tgagaacggt gaatttctga ttgaagccag cgatctcggc
    12241 gtggtgaata tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc
    12301 tacaacgcgg tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg
    12361 gtggagcttt cccgcgactg gctggtgaat ctgcttaatc agtgcgatga gctgggcatt
    12421 cgcaaccagt ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgcc
    12481 cgctgcttta ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt
    12541 aagtatccga acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat
    12601 ggcattcaga ccatgagcgg ctacgtttac aacctcggta acgagctggc atccatgcag
    12661 ggattggttg atgtagtccg cctgtcaccg cagggcactg acactttcgc gatgctcgac
    12721 gccttccgcg ctaatgaaaa tggcgctgtg ccactgccgt tgacggcaaa cagcgactgt
    12781 aacggctact ggcggcggct ggcaggactg gagctgcaag cctaagtaaa tagctcactt
    12841 tgttaacaac tttaactatt ctttaatgca gtattaaaga ttaatcggta acaaagtgag
    12901 ctgttatgac tgataaaacc attgcgtttt cgctactcga tctggccccc atacccgaag
    12961 gctcttcagc gcgggaagcg ttctcccact ctctcgatct cgcccgtctg gctgaaaagc
    13021 gcggctatca tcgctactgg ctggcagaac accacaatat gaccggcatt gccagtgctg
    13081 caacatcggt attgattggc tatctggcgg cgaataccac cacgctgcat ctggggtctg
    13141 gcggcgtgat gttgcctaac cacttaccgt tggtcattgc cgaacagttc ggcacactca
    13201 atacgctcta tccggggcga atcgatttgg ggctgggtcg tgctccgggt agtgaccaac
    13261 ggacaatgat ggcgctacgt cgtcatatga gcggcgatat tgataatttc ccccgcgatg
    13321 tcgcggagct ggtggactgg tttgacgccc gcgatcccaa tccgcatgtg cgcccggtac
    13381 caggctatgg cgagaaaatc cccgtgtggt tgttaggctc cagcctttac agtgcacaac
    13441 tggcggcgca gcttggtctg ccatttgcgt ttgcctcaca cttcgcgccg gatatgttgt
    13501 tccaggcgct gcatctttat cgcagtaact tcaaaccgtc ggctcggctg gaaaaaccat
    13561 acgcgatggt gtgcatcaat attatcgccg ccgacagcaa ccgcgacgct gaatttctgt
    13621 ttacctcaat gcagcaagcc tttgtgaagc tgcgccgtgg cgaaaccggg caactgccgc
    13681 cgccgattca aaatatggat cagttctggt caccgtctga gcagtatgac gtgcagcagg
    13741 cgctgagtat gtcgttggta ggcgataaag cgaaagtgcg tcatggtttg cagtcgatcc
    13801 tgcgcgaaac cgacgctgat gagattatgg ttaacgggca gattttcgac caccaggcgc
    13861 gcctgcattc gtttgagctg gcgatggatg ttaaggaaga gttgttggga tagtgtgtct
    13921 taacgcggga agccttatcc gagctggcaa cgctgtccta cgtaggcctg ataagcgaag
    13981 cgcatcaggc attgtgtagg cagcagaaat gtcggataag gcaccgctga ttactgatac
    14041 accggcagta aattaaagct cgataaaata tgcaccagtg cgttgccgac gccaaacacc
    14101 agaatcagcg caatcatcgg cttgccaccc cagacgcgga atttcgggct gccaaagcgt
    14161 ttacgcgatg cgcgggccag cagtgccgga acaattgccg cccagatggt agccgctaaa
    14221 ccagcataac caatggcata caggaatccg ttcgggaaca gcagaccacc cacaacaggc
    14281 ggggcaaagg tcagcaatgc cgttttcaag cggcccacag ccgagtcgtc gaaaccaaac
    14341 agatctgcca gatagtcaaa caaacccagc gttacgccaa ggaacgaact cgctaccgca
    14401 aagtttgaga acacgaccag cagaaggtcc aggctacggc tgttcagcac gccgcttaat
    14461 gcttgtacca acacatcaat attaccgccc ttctcggcga tgccgataaa ctccggacga
    14521 gggatgttgc ccattgtcgc cagcaaccag atggtataca gcgccagtgc catcagcgta
    14581 ccgtacacca ggcatttcac gatggttttc ggatctttgc cgtaatactt catcaggctt
    14641 ggcacgttac cgtgataacc aaacgacgcc aggcagaacg gcagggtcat cagcaaatac
    14701 ggcgcataag atgcattgct ttcggcaacg ttgaacaatg tcgcaggctg cacatgcccc
    14761 agcaggctac cgaaggtgag gaagaaggta atgactttcg cccccagcac aatcgccgtc
    14821 atgcggctga cggctttagt actcaaccac accacaaacg ctaccagcag tgcaaaacca
    14881 aaacccgccg cccgtgccgg gacgtttagc gacatctctg cgaaggtgtg atgcagaatc
    14941 gaaccactgg cagaaatata ggcataggtc aggatataga gcacaaaggc aatggaaatg
    15001 ccgttgacca cgttccagcc tttgcccagc aaatctttgg tgatggtgtc aaaactcgaa
    15061 ccgattcggt aattcaggtt agcttccaga atcatcaagc cggaatgcag catacagaac
    15121 caggtaaaga tcagcgctgc cattgaccag aaaaaccacg ccccggacat gaccactggc
    15181 agagaaaaca tccctgcgcc aataatggtg ccgccgataa tcaccacgcc gccaagcagc
    15241 gacggtgacg tttgggtggt ggttagtgtt gccatgaggg cttctctcca gtgaaaaata
    15301 gtgcgactgc gttgttatgc attgcactgt accagtacat gagtacaaaa gacagaaaaa
    15361 aagccccgat ggttaaaatc ggggctgtat atattatttt acagattgtg ttcgctgttc
    15421 agcgatgatt acgcatcacc accgaaacga cgacgaccgg tagagtcatc acgacgcggt
    15481 gcgcggcctt cacgacgttc gccgctaaaa cgacgaccat caccacgacc accttcacgg
    15541 cgttcaccgc tgaagttacg accgccttca cgacgctcgc caccgaaacc acgaccaccg
    15601 ccacgacgct caccgccagt atgcggctgt gcatcgccca gtaactgcat gttcatcggc
    15661 ttgttgagaa tgcgagtgcg cgtaaagtgt tgcagcactt cacccggcat acctttcggc
    15721 agttcgatgg tggagtgaga agcaaacagc ttgatgttac cgatgtaacg gctgctgatg
    15781 tcgccttcgt tagcaatcgc gccaacgata tgacgaactt caacaccatc atcgcggccc
    15841 acttcaatgc ggtacagctg catatcgcca acatcacgac gttcacgacg cggacgatct
    15901 tcacggtcgc cacgcgggcc acggtcgtta cgatcgcgcg gaccacggtc atcacggtca
    15961 cggaattcac gtttcggacg catcggcgca tctggcggaa caatcagagt acgttcaccc
    16021 tgtgccattt tcagcagtgc cgcagccaga gtttcgagat ccagctcttc accttctgca
    16081 gtcggctgaa ttttgctcag cagtgcgcgg tattgatcca gatcgctgct ttccagctgc
    16141 tgctgtactt tagcggcgaa tttttccaga cggcgtttgc ccagcagttc tgcgttcggc
    16201 agttctactt ccggaatagt cagcttcata gtacgttcaa tgttgcgcag cagacgacgc
    16261 tcgcggttct caacgaacag cagcgcgcgg ccagcacgac ccgcacgacc ggtacgaccg
    16321 atacggtgaa cgtaagactc agaatccatc gggatatcgt agttaaccac caggctgata
    16381 cgctcaacgt ccaggccacg ggccgcaacg tcggtcgcaa tcaggatgtc cagacgaccg
    16441 tctttaaggc gttccagtgt ctgctcacgc agcgcctggt tcatgtcacc gttcagcgcg
    16501 gcgctattgt agccgttacg ctcaagagct tcagccactt ccagagtcgc gtttttggta
    16561 cgaacgaaga taatcgccgc atcaaaatct tccgcttcca ggaaacgtac cagtgcttcg
    16621 tttttacgca taccccacac agtccagtag ctctggctga tgtcaggacg ggtagtcacg
    16681 ctggactgaa tacgcacttc ctgcggctct ttcataaagc ggcgggtaat gcgacgaatc
    16741 gcttccggca tggttgcaga gaacagagcg gtctgatgac cttccgggat ctgcgccata
    16801 atggtttcaa cgtcttcgat gaagcccatg cgcagcattt cgtcagcttc atccagaacc
    16861 agaccgctca gtttagagag gtccagagtg ccacgtttca ggtggtccag cagacggccc
    16921 ggagtaccga caacgatctg cggcccctga cgcagggcgc gtaattgcac gtcataacgc
    16981 tggccgccgt acagggcaac cacatttacg ccgcgcatgt gtttagagaa atccgtcatt
    17041 gcttcagcaa cctgtaccgc cagttcgcgg gtcggtgcca gcaccagaat ctgtggtgct
    17101 ttcagctcag gatcaagatt ctgcagcaga ggtaaagaga atgctgcagt ttttccgctc
    17161 cccgtctggg ccatacccag aacgtcgcgg ccattcagca gatgtggaat acactctgcc
    17221 tgaattggag atggtttttc gtaacccaga tcgttaaggg cttcaaggat aggagccttc
    17281 aggcccagat ctgcaaaagt ggtttcgaat tcagccatgt agtacgtgtg cctcaaaatt
    17341 aatggcggcc agtctacata actcatcatg aaattgatca gcaattttca ttgaaaagtg
    17401 tgaaccggct caaagtaggt gtattaacga acaacaacgc cctcacccgt taaggtgatg
    17461 gcaatcaaaa aagattacgg gctgatgtgt acgtcagcta ttgctggtcc gattctgcca
    17521 ggtcatcttg gtcctggccc aggagcgata attccaacaa tgcgtatcgg tgctcaacaa
    17581 agttatgaac gttgttggca accgccagtt tgaacagtgc cgtggcgctg tccaaatccc
    17641 ccagacttag gtagtactta cctaaataga agttggtttc actgagatgc tcagcgagcg
    17701 aggtgttatc cgttgcgtcc gccttgagcc tttccattaa cgtttgttcg ctaatgttgc
    17761 ccaggtagaa ctcgacaatg ttccatcccc actgttcctt atccgatttt tcgaagtgct
    17821 gtttcaatac ttctttagcc tgcttctcat cgagcttctg ctcggcgaga taaagccaca
    17881 gactacggaa aggatcattg ggatcgtctt gataaaacgc cagcagatca tcttgcgcta
    17941 acttgtcacg accgccgtaa tataatgcaa tcccgcgatt caagtgcgcg tagttgtaag
    18001 ttggatcaag ctcaagtaca gaatcaaacg cttcataggc agcatcaaaa ttgcctgcct
    18061 gcgttaaata tatgcctaag taattgaata cttcaggcat atccggtcgg attgccagcg
    18121 cttgcgaaaa atcgttacgc gctaatgccc tcagaccgag actatcatac aacactccgc
    18181 gctcatataa aagctgtgcg cgttcgtcat cggttaaagc ccgactggca aggatttgtt
    18241 ccatacgtgc cagaatcact tcctgctgta aagtcggttg caatggtacc gcgaggactt
    18301 cacttttacg ccaggaagta ttactgcatc ctgcaagcgt aagtgctgtc gcaacgaaac
    18361 accagcgcaa aaaaggcttc atttcccact cccgaagacc acggttgaat gaacgtcctg
    18421 ttcccggttg ctaacaaggc gtcctgcccg gttaaaagcc ccccgccgca gcggagggca
    18481 aatggcaacc ttactcgccc tgttcagcag ccggagcttc cggtgctgca gcaggttgag
    18541 actgctcagt cgcttcttta atgctcagac ggatacggcc ctggcgatca acttccagaa
    18601 ctttcaccgg tacttcctga cccatctgca ggtaatcggt cactttctca acgcgtttgt
    18661 cagcgatttg agagatgtgt accagacctt ctttaccgcc gccgatggca acaaatgcgc
    18721 caaagtcaac gatacgggtc actttaccag tgtagacgcg gcccacttcg atttctgcag
    18781 tgatctcttc gatacgacga atagcatgtt tcgctttctc gccgtcggtc gctgcaatct
    18841 tcacagtacc gtcatcttcg atttcgatgg tggtgccagt ttcttcggtc agggcacgga
    18901 taacagagcc gcctttaccg ataacgtctt tgatcttgtc cgggttgatc ttgatggtat
    18961 ggatacgtgg tgcgaactca gagatatcgc cacgcggcgc gttgatcgcc tgttccatta
    19021 cgcccaggat atgcagacgc gcacctttag cctggttcag cgcaacctgc atgatctctt
    19081 tggtgatacc ttcaattttg atatccatct gcaatgcaga gataccctcg cgggaacccg
    19141 caactttgaa gtccatatcg cccaggtgat cttcgtcgcc caaaatgtca gacagtacaa
    19201 cgtagttgtc gccttctttc accagaccca ttgcgatacc cgcaacggca gctttgatcg
    19261 gcacacctgc gtccatcagc gccagagacg cgccgcacac ggaagccata gaagaggaac
    19321 cgttggattc agtgatttca gacacaacac gtacggtgta cgggaatttg tccatatccg
    19381 gcatgactgc cagcacgccg cgcttcgcca gacgaccgtg accaatttca cgacgcttcg
    19441 gagaaccgac catgccggtt tcgcctacgg agtacggagg gaagttgtag tggaacagga
    19501 aggtatcggt acgttcgccc atcagttcat caagaacctg cgcgtcacga gcagtaccca
    19561 gcgttgcggt aaccagcgcc tgcgtttcac cacgggtgaa cagcgcagaa ccgtgagtac
    19621 gcggcagcac gccagtacgc acatccagac cacggatcat atctttttca cgaccgtcga
    19681 tacgcggttc gcctgccagt acgcggctac gaacaacgtt tttctcgatc gcgtgcagaa
    19741 tttcacccag ttcgttttcg tccagggttt cgtcttcagc aagcagcgtc gcgatggttt
    19801 cagatttgat gacatcaacc tgcgcataac gctcttgttt gtcggtgatg cggtaagcat
    19861 cgctcaggcg agcttcagcc agtgcagcaa cgcgcgcgtt cagcgcttcg tttaccggct
    19921 ccggctgcca gtcccaacgt ggtttaccgg cttctttcac cagttcattg atgttctgaa
    19981 taacaacctg ctgttgttca tgaccgaaca ctactgcgcc cagcatctgg tcttcgctca
    20041 gcagttcagc ttcagattca accatcagta cagcggcttc agtaccggca acaaccagat
    20101 ccagtttgct ctctttcaac tcgtcctgag tcgggttcag tacgtactgg tcattgatgt
    20161 aacctacgcg agcagcacca atcggaccat tgaacggaat accagacaga gacagtgctg
    20221 cggaagcacc aatcatcgcg acgatatccg ggttaacttg cgggttaaca gaaaccacgg
    20281 tggcgataac ctgaacttcg ttgacgaagc cttccgggaa cagcggccga atcgggcggt
    20341 caatcagacg cgcgatcagg gtttcgcctt cgcttgggcg gccttcacga cggaagaagc
    20401 tacccgggat acgaccagca gcgtaggtac gctcctgata gttaacggtc agtgggaaga
    20461 agtcctgacc tggtttggct tttttctggc caacaacggt aacaaatacc gcggtgtcat
    20521 ccatgctaac cataacagcg gcagtagcct gacgagccat catgccggtt tccagagtca
    20581 cggtgtgttg gccgtactgg aatttacgaa cgatcggatt aagcaatgta atatcctttc
    20641 tctttcttag acagtacctt acggcactgg tgttaatacc cgatcttctg cgcatcctcg
    20701 cgactaatga caaccctaac ccagctctat gtgggtaaag cctctcatta gccgcgcgaa
    20761 cctctgcaac ggaagatcat tcatagcaac aatacattag tttccagtga attgctgccg
    20821 tcagcttgaa aaaaggggcc actcaggccc ccttttctga aactcgcaag aattagcgac
    20881 gcagacccag gcgctcgatg agctgggtgt aacgtgctac gtctttacgt ttcaggtagt
    20941 cgagcagttt acgacgctga gaaaccatgc gcagcagacc acgacggctg tggtgatctt
    21001 ttttgtgctc tgcaaagtgg ccctgcaggt ggttgatctg tgcagtcagc agtgctacct
    21061 gaacttcagt agaaccggtg tcgtttgcgt cacgaccaaa ctcagaaacg attttagctg
    21121 ttgcttcagt acttagagac attttaaaac tccaaagtat atagaatgaa aggacgccga
    21181 tctctaattc agcgatccca gtgtacgtta cgcaaagtgt taaacaattt acgcgacgtt
    21241 aagcggcagt attctactcg tagcgacctg ttatcgcaag acggttaaca ttacgccggg
    21301 tattcaacca ccaggcgacg aggcgcaacg cggccttcat cgtcaatttc gcccataccg
    21361 ataaatttgc cgttctcacc ttctgtaacg cgaaccagtc cttccagcgg cgcaccggat
    21421 gtacgaactg ggttaccatt tttgaagtaa acagaagacg ttaacggaag attcaccacc
    21481 gggtagtccg aagctggact gtccattggc atcagtaatg gatcaagtaa ctccgcagcc
    21541 ggaatatcct gctgttcggc ttgttcgacc agttcacgca gatgttccag ggtcacaatc
    21601 cgttcaaccg gatatttact taccgccaga cgacgcaggt aaataacatg cgcgccacaa
    21661 ccgagttttt cacccaggtc atcaatgatg gtgcggatat aagtgccttt tgagcagtga
    21721 atttccagct ccagctcatt gccttcgtgg cgaataaaca gcaattcata aacggtaatc
    21781 gggcgcgctt cacgcggtac ttcaatgccc tgacgcgcat attcgtacag ttttttaccc
    21841 tgatatttca gtgctgaata catcgaaggg atctgttcga tatcgccacg gaaagtatcc
    21901 agcgccgccg ccagttgctc tgcgctaaag gttaccggac gttcttcaac gatttgtccg
    21961 tcggcatcag aagtatcggt acgctgtcca agacgcgcaa tgacccgata gcgtttgtcg
    22021 gagtccagca gatactggga aaacttcgtc gcttccccga ggcaaattgg caacatgcca
    22081 gttgccagcg ggtccagcgc accggtatgc ccggcacggt tggcgttata aatccgtttc
    22141 actttttgca gcgcatcatt gctggacata ccctgcggtt tatccagcaa caaaacgccg
    22201 ttaatgtcgc gaccgcgacg acgaggacga ctcattagtc ctccttgctg tcgtccgggt
    22261 taacacgacg ttcttcgtca tgtttgacca cgctggtcac caggtttgac atgcgcatcc
    22321 cttcaaccag agagttgtcg tagaagaagg tcagttccgg cacgatacgc aggcgcatcg
    22381 ctttccccag caggctgcgg atgaaaccag aagcttcttg caacgctttg atgcccgctt
    22441 taaccgcgtc ttcatctttg tcgttgagga acgtcacata tactttggca tacgccaggt
    22501 cgcgagacat ttcgacaccg gaaacggtgg tcatcatgcc caggcgagga tctttaattt
    22561 cacgctgcag gatgagagcg atctcttttt gcatttcctg cgctacgcgc tgcgggcgac
    22621 caaattcttt cgccataata aattctcctg acaaaaaagg ggctgttagc ccctttttaa
    22681 aattaatttc aggtggaagg gctgttcacg ttggcctgat aagacgcgcc agcgtcacat
    22741 caggcaatcc atgccggatg cggcgtaaac gccttatccc gcatggaacc ctaaaaacct
    22801 taagcgatgg tacgttggat ctcgatgatt tcgaatactt cgatcacatc gccagtgcgg
    22861 acgtcgttgt agttcttaac gccgatacca cattccatac cgttacggac ttcgttaacg
    22921 tcatctttga agcggcgcag ggactccagc tcgccttcgt agataaccac gttgtcacgc
    22981 agaacgcgga tcgggttgtg acgtttaacc acaccttcgg taaccataca gcctgcgatg
    23041 gcaccaaatt tcggcgattt gaacacgtca cgaacttccg ccagaccgat aatctgctgt
    23101 ttcagttccg gagacagcat accgctcatc gccgctttca cttcgtcaat caggttatag
    23161 atgacggagt agtaacgcag atccaggctt tccgcttcaa tcactttacg tgcagaggca
    23221 tcagcacgta cgttgaagcc aaccaggatg gcgttggacg ccgcagccag ggtggcgtcg
    23281 gtttcggtga taccacctac gccagaaccg atgatcttca ctttaacttc gtcagtagac
    23341 agtttcagca aggagtcgga gatcgcttcc acagaaccct gtacgtctgc cttcaggacg
    23401 atgttcactt cgtgaacttc gccttcggtc atgttggcga acatgttctc gagtttagat
    23461 ttctgctgac gcgccagttt aacttcgcgg aatttaccct gacgatacag tgcaacttca
    23521 cgcgctttct tctcgtcacg tacaacggta acttcatcac ccgcagccgg tacgccggac
    23581 aggccgagga tttccaccgg aatggacgga cccgcttcca gcacttcctg acccagttcg
    23641 ttacgcatcg cacgaacacg accgtattcg aagccacaca gaacgatatc gcccttgtgc
    23701 agagtacctt cacgtaccag aacggtagca accggaccac gacctttatc gaggaaggat
    23761 tcgataaccg caccgctcgc cataccttta cgtaccgctt tcagctccag aacttccgcc
    23821 tgcagcagga tagcgtccag cagttcatcg ataccagtac ccgctttcgc agacacatgt
    23881 acgaactggc tttcaccgcc ccactcttcc ggcaggatgc cgtactggga gagttcgttc
    23941 ttaacgcgat ccggatcggc ttctggttta tcgatcttgt tcactgcaac gaccaccgga
    24001 acctgcgccg ctttcgcgtg ctggattgct tcgatcgtct gcggcattac gccgtcgtcg
    24061 gcagcaacaa ccaggactac gatgtccgtt gcctgcgcac cacgagcacg cattgaagta
    24121 aacgcggcgt gccccggggt gtccaggaag gtgatcatgc cgttttcagt ttcaacgtgg
    24181 tatgcaccaa tgtgctgggt aatgccgccc gcttcgccag aggccacttt cgttgaacga
    24241 atgtagtcca gcagagaggt tttaccgtgg tcaacgtgac ccatgatggt cacaaccggc
    24301 gcgcgcggtt cagccgcagc acccgtgtca cggtcgctca ttaccgcctc ttccagctcg
    24361 ttttcacgac gcaggataac tttatggccc atctcttcag caaccagctg tgcggtttcc
    24421 tgatcgataa cctggttgat ggttgccatt gcgcccagtt tcatcatcgc tttgatgacc
    24481 tgagagcctt taaccgccat cttgttcgcc agttcgccaa cggtgatagt ttcgccgatc
    24541 acaacgtcac ggttaacggc ctgagcaggc ttctggaagc cttgctgcag cgaagaacct
    24601 ttacgttttc cgcctttacc gccacgtact gctgcgcgtg cttcttcacg atcagctttt
    24661 gattcagcgt gtttgttgcc tttcttcgga cgcgctgctt tcgcgttacg accacggcca
    24721 cggccgcctt cgacttcacg atcgctttcg tcttctgcct ggcgagcatg ttgagaagta
    24781 gtgacgtgat aatcgctgga atcttcagtc ggttccgcgt tatcagtcca tttgttttct
    24841 tccgccatac gacgtgcttc ttcagcaacg cgacgtgctt cttcttcgag tttacgacgc
    24901 gcttcttctt cagctttacg cttgagctct gcagcttcct gctcacggcg ggctttttca
    24961 gcctgggcgt ttttagtcat atcgtcttgt tgattgctca ctttgtcttt ttccgcagct
    25021 tcacgtttcg cttgttcagc agcttcacgc ttagcttgtt ctgcggcctc acgttcagct
    25081 ttttgttgcg cctcgcgttt agccgattct tctgcctcac gacgggcttg ctcttccgct
    25141 gcaaggcgtt cagcctcttg cggatcgcgt ttcacaaagg tgcgtttctt gcggacttcg
    25201 atttgtaccg atttgctttt tccaccggta ccaggaatgt taagggtgct gcgtgtttta
    25261 cgttgcagcg tcaatttgtc cgggcctgaa tttttctgat tcaggtggtc aatcaaagtc
    25321 tgtttctctt gtgcagacac agagtcgtca gcagacttcc ggatacctgc atcagcaaat
    25381 tgctgtacca ggcgttccac ggaggtctgt cgctctgcgg ccagcgtttt aatcgttaca
    25441 tctgtcatgc tgttccttcc tgctacagtt tattacgctt cgtcaccgaa ccagcaaata
    25501 ttacgggcag ccataatcag tgctccggct ttttcgtcgg tcaacccttc gatatcagcc
    25561 agatcatcaa tgccctgttc ggcgagatct tccagcgtac aaacgccacg ggcggccagt
    25621 ttgaatgcca aatcacgatc taccccttca aggttcagca gatcgtcagc cggtttgtta
    25681 tcaccgaggc tttcttcctg ggcctgtgca atggtggcca gtgcattttt agcacgctcg
    25741 cgcagtgctt caacggtcgg ctcatcaagg ccttcgattt ccaacagctc tttcatcggc
    25801 acataggcca gttcttccag cgtcgagaaa ccttcttcta ccagaacagt cgcgaagtct
    25861 tcgtcgatgt cgagatattt ggtgaaggtg tcgatcgctg cgtgcgcttc cgcctgatgc
    25921 ttagcctgca ggtcgtcaac ggtcattacg ttgagttccc aaccgctcag ctgcgaagcc
    25981 agacgcacgt tctgaccgtt acggccaatc gcctgcgcca ggttaccagc ttcaacggcg
    26041 atatccatgg tgtgtttatc ttcatccacc acgatagaag caacgtctgc cggtgccatt
    26101 gcgttaatca caaactgcgc cgggttatca tcccacagga cgatatcgat acgctcgccg
    26161 cccagttcag tagaaaccgc ctgaacacgc gcgccacgca tacctacgca agcacctacc
    26221 ggatcgatac gtttatcgtt ggttttcacc gcgattttcg cacgagaacc cgggtcgcga
    26281 gccgctgctt taatttcaat cacttcttcg ccgatttccg gcacttcaat acggaacagt
    26341 tcgatcagca tttccggctt ggaacgagtg acgaacagtt gcgcgccacg cgcttccggg
    26401 cgaacggaat aaagcacgcc acgaacgcgg tcgccagggc ggaagttttc acgcggcagc
    26461 atatcttcgc gcaggatcac ggcttcagcg ttgttgccca gatccagaga gatgttgtcg
    26521 cggtttactt ttttcaccac gccggtgatg atttcacctt cgtgttcacg gaactgatca
    26581 accaccatcg cacgttcggc ttcacgcact ttctgcacga taacctgttt tgccgtctgg
    26641 gtggtgatac ggtcaaaggt aacagactca atctgatctt caacgtaatc gcccaagttc
    26701 aggctttcat cttcataacg tgcggcttca agggtgattt ccttggtcgg ctgggtgact
    26761 tcatcaacaa ctaaccagcg acggaaggtg tcaaaatcac cgcttttgcg atcgatctgt
    26821 acacggacgt cgatctcttg ctcatatttt ttctttgttg ctgtcgccag cgcgctttcc
    26881 aatgcttcga aaatcttctc gcgaggtagc gccttttcat tggatacggc ttcaactaca
    26941 gccaaaattt ctttgttcat cgcgggcttt tcacctcatc cagactatta aaagtgggga
    27001 accaggttcg ccttctggat attactcagc gcgaacactt catctttacc ttcgacggta
    27061 actgtgatca tttcaccgtc taccgctttg ataacgccct gccatttacg acggttttgt
    27121 accgccatac ggagaaccag agtcacctct tctccgacaa aacgggcgta gtgttcagcc
    27181 gtgaacagtg ggcgatcgag acccggtgag gagacttcca ggttataagc aacggtgatg
    27241 ggatcttcaa catccagcac agcacttacc tggtggctca catcagcaca atcatcaaca
    27301 ttgatgccat cttcactatc aatatagatg cgcagtgtgg atgtgcgacc gcgaataaat
    27361 tcgatgccta ccagttcaaa acccagggcc tcaactggcg cagtaatcat ctctgttaat
    27421 ttttgctcta atgtggacaa gcccaccccc aagacataaa aaaagggcct aaagcccagt
    27481 tattctgtag tcagataaca aaaaaccccg ataaatcggg gctttatata actgaaccct
    27541 ataaccgcaa ctgcggtctg gagcactttc cagaaggatt ttttcaaatc ccactacgaa
    27601 ggccgaagtc ttcacagtat atttgaaaaa ggactctaag ggaaagtggt tgcgggggcc
    27661 ggatttgaac cgacgacctt cgggttatga gcccgacgag ctaccaggct gctccacccc
    27721 gcgcctgaaa cgtggcaaat tctactcgtt ttgggtaaaa aatgcaaata ctgctgggat
    27781 ttggtgtacc gagacgggac gtaaaatctg caggcattat agtgatccat gccacatttt
    27841 gtcaacgttt attgctaatc acgtgaatga atatccagtt cactttcatt tgttgaatac
    27901 ttttgccttc ccctgctctc ccttaagcgc attattttac aaaaaacaca ctaaactctt
    27961 cctgtctccg ataaaagatg attaaatgaa aactcattta ttttgcataa aaattcagtg
    28021 agagcggaaa tccaggctca tcatcagtta attaagcagg gtgttatttt atgacgacga
    28081 ttctcaagca tctcccggta ggtcaacgta ttggtatcgc tttttccggc ggtctggaca
    28141 ccagtgccgc actgctgtgg atgcgacaaa agggagcggt tccttatgca tatactgcaa
    28201 acctgggcca gccagacgaa gaggattatg atgcgatccc tcgtcgtgcc atggaatacg
    28261 gcgcggagaa cgcacgtctg atcgactgcc gcaaacaact ggtggccgaa ggtattgccg
    28321 ctattcagtg tggcgcattt cataacacca ccggcggcct gacctatttc aacacgacgc
    28381 cgctgggccg cgccgtgact ggtaccatgc tggttgctgc gatgaaagaa gatggcgtga
    28441 atatctgggg tgacggtagc acctataaag gaaacgatat cgaacgtttc tatcgttatg
    28501 gtctgctgac caatgctgaa ctgcagattt acaaaccgtg gcttgatact gactttattg
    28561 atgaactggg cggccgtcat gagatgtctg aatttatgat tgcctgcggt ttcgactaca
    28621 aaatgtctgt cgaaaaagcc tactccacag actccaacat gcttggtgca acgcatgaag
    28681 cgaaggatct ggaatacctc aactccagcg tcaaaatcgt caacccgatt atgggcgtga
    28741 aattctggga tgagagcgtg aagatcccgg cagaagaagt cacagtacgc tttgagcaag
    28801 gtcatccggt ggcgctgaac ggtaaaacct ttagcgacga cgtagaaatg atgctggaag
    28861 ctaaccgcat cggcggtcgt cacggcctgg gcatgagcga ccagattgag aaccgtatca
    28921 tcgaagcgaa aagccgtggt atttacgaag ctccggggat ggcactgctg cacattgcgt
    28981 atgaacgtct gttgaccggt attcacaacg aagacaccat tgagcagtat cacgcgcatg
    29041 gtcgtcagtt gggccgtctg ctgtaccagg ggcgttggtt tgactcccag gcgctgatgc
    29101 tgcgtgactc tctgcaacgc tgggttgcca gccagatcac tggtgaagtt accctggagc
    29161 tgcgccgtgg gaacgattat tcaatcctga ataccgtctc agagaacctg acctacaagc
    29221 cagagcgtct gacgatggaa aaaggcgact cggtgttctc gccagatgat cgtattggtc
    29281 aattgaccat gcgtaatctg gatatcactg atacccgaga gaaacttttt ggttatgcca
    29341 aaactggcct gctttcctcc tctgccactt caggcctgcc gcaggtggag aatatggaaa
    29401 acaaaggcca gtaattcgac atcaaccctg cccttcaggg gtggggttac cttgcttatc
    29461 ctctgtaaac tcctttctga gcatcacata atcgaaaaca tcatggtttg catccacaac
    29521 ccgtgagtct cctttatagt gtgcaatcac ttcctcaagc gtttgcggct gttccggctt
    29581 tgttaccccc atccacgcat taataaggta attattgtaa gctgtcgaaa agatattgtt
    29641 ttccgttgtg cgatccacgc catccccaag aacggggcta taccagataa acatcgggac
    29701 atgatatgcc tgctggctag cctccctgcc tccatgaaaa tagacgttct tcttcgtagg
    29761 gtcacgttcc aggccgtggt cggcaaaata catgaccgag gcgcggcgat cttttaataa
    29821 ttcaaaaacc tgacctagca aactatctgt gtaatgaatg gagttgtcat agcaggcatc
    29881 ctgatcgtcc tgcggttgaa acacggcgct ggattgcgga taggcgctac aagccggttc
    29941 atggcttcca tttaaatgaa gaacaatcag tttcttttgc tgcgtatttt gctgtaatgc
    30001 ttgcgataaa tgcggcaaca acaattcatc aaatcctctg acataaactg tttccatggc
    30061 gcgcatggcg atactggtaa ctgctgtacc attctgccga aaagcggatt gcgagcttag
    30121 ccagaaagtc tgaaatcctg cttgattagc catattaata atgttgtcgg ggtaattatg
    30181 aatgtcatga ctcaaaacag aatcagcagt taaagaaagg ggaaccgaca gcgcagtgta
    30241 aggtgcgccg cttattgctt gattaaacag tttgatctgt tttctttgtg cttcaacttg
    30301 cggtgtcgta gagcgtgtat atccatacaa agacatattg tcgacacgta cagactcccc
    30361 cacaatcaac acgtaggtat caatacttgt atccctgact gataattgaa aatacggcac
    30421 cgtgtttgca attgagagta atctttgatg cgctttcgct gctaaagcaa aatagttgag
    30481 attgaaaaac ggcgtatagg tagcaaatca cgacgctagt atatatggac tgaacgcttt
    30541 tttatttttt gcatctttat aagcaaattg acaagcggaa aataaactgc ccgaaatgac
    30601 aatcagcaat aatattcctg tcactttttt tgtcggcaag gaaacatcat attttattat
    30661 tactgccaaa aaaagaaggg ataaaaaagc aaaagcacat agataaggga tatacatccc
    30721 cagcattttc acaacttcat ccggatcact ctgcagcaca ctaatcgcaa atccatcatt
    30781 gaaagttgta ccaaaggtac accatgaata gaggctaata ctcatgtctg ccgcagtcag
    30841 taacacaaat ggtgcggcaa caagcaggcg aagcggaagg gatgatatac gtttaacaac
    30901 caggatggtc aggacgaaga acagcaatcg ctgaaccata tgcgctccgg aagaggcgac
    30961 taagttcgta ataccaaaaa caataacaca aagatacaac aaccaatgag atttaaaatc
    31021 ttctagcaaa tttgttgaat actgtcatcc agaaatccaa gttcacaacc aatataaaca
    31081 attatcaaca atgttcaatt ttatatcaga catgtttttc aactctgatc catatcagac
    31141 cgaaacactg ttagtttttt ctttttaaat gatgcgacca ttgcgttctc agtaattaaa
    31201 taacattatc aatgcgtctc taaacattac gcagaacgta gccatcagta gttatactga
    31261 caggagcagg atggtttatc gagaagtaaa catatccgta ttcgtaccgg aactggagtt
    31321 gacacaataa agtgccaatt atgtcagcag tagggaaaag tttataacta aggcgtaccg
    31381 gcaccatcgt ttcaaggtac cagctacgag taaagcaact ggacgagata cagatacctg
    31441 acaaccattc atcagaccag gaccaaaacg aaaagagacg cttttcagcg tctcttttct
    31501 ggaatttggt accgaggacg ggacttgaac ccgtaagccc tattgggcac taccacctca
    31561 aggtagcgtg tctaccaatt ccaccacctc ggcacggata ctacttttta gttcgggata
    31621 tcgctggtcg gcttagccgg agcagctggc tgagtttgtt cggttttcgc cggtgcactc
    31681 agattttccc attcgctacc tctattggtt ttgttgctat tgatgttacc cagcaccaga
    31741 ctgatgatga agaataacgt tgccagcagc gccgtcatgc gggtcatgaa gttaccagaa
    31801 ccacttgaac caaacagcgt agcggaagcg cctgctccga aggaggctcc catatcagcg
    31861 cctttacctt gctgcagcat gatcagacca acaaggccaa ttgccacaat aaggaaaact
    31921 actaaaagag cttcgtacat aatcaacctg ttccttgcgg agttgccgcg taccaatgct
    31981 tcaaccaata aagcgggagt ttttaatctt tcccactgaa gcgggtgtga atactaacca
    32041 aaacgaatga ccttcgcaag ggcaatttta tcgcattgta tcaactgcgg aaaaaaacag
    32101 caaaaccgat gtgttcgccg agaaaaaagg cgacaatcgc cgccttttta gccagttatc
    32161 taacgcttta aacggctttt actgcatcgg cgatgcggtg tgcaaattca gtcacctgcg
    32221 cttcgtcttc gccttccacc atcacgcgaa ttaacggttc ggtgccggat ttacgcagca
    32281 acacgcgtcc acggttgccc agcgcagctt caacctctgc ggtcacggct ttaactgact
    32341 catgctcaag cggatcgccg ctacctgcgg tgtaacgtac gttaaccaga atctgcggga
    32401 acattttcat gccgctgcaa aggtcgtgca ggctcatatg gttacgtgcc atcgccgcca
    32461 gcacctgcaa gccagcaacg atgccgtcac cggtagtggt tttatccagc aggatcacat
    32521 gaccggaatt ctctgcaccg atacgccagc ctttctcctg cattttttcc agtacgtagc
    32581 ggtcacccac tttcgcgcgc gcaaatggaa ttcctaactg tttcagcgcc agttcaagcc
    32641 ccatgttgct catcaatgta cccacagcac caccacgcag ctggccctga cgaagacctt
    32701 cacgcgcgat gatatacatg atctgatcgc catcgacttt attgccttca tggtcaacca
    32761 taatcacgcg atcgccgtcg ccgtcgaagg caataccgag atccgctttt tcagccagca
    32821 cacgagcctg gagcgcgcga acgtcggtag cccccacttc ggcattgatg tttacaccgt
    32881 ttggctcaca accgatagcg ataacgttcg cccccagttc gcgcagcacg ttcggcgcga
    32941 tgtgataagt cgcaccgttt gcacaatcca ccacaatctt cagttcactg aggctaagtt
    33001 cgttcgggaa cgtggctttg caaaactcga tatagcgacc cgcggcatca acgatacggc
    33061 tggctttacc cagttcagcc gaatcaacgc agctgatctc cttttccatt tccgcttcga
    33121 tggcctcttc taccgcatcc ggcagtttag tgccgtcgat agagaagaat ttaatcccat
    33181 tatcgtagaa cgggttatgc gatgcagata tcacaattcc ggcctctgcg cggaaggtac
    33241 gcgtcagata agccacggcc ggtgttggca tcgggccagt gaagagtgcg gaaaggcccg
    33301 ctgccgccag acccgcttcc agtgctgact ccagcatata gccagaaata cgcgtgtctt
    33361 taccaataat aatcttacgg gagccgtggc gcgccagcac tttacccgcg gcccaaccca
    33421 gcttaagcac aaaatcaggt gtgatcggcg catcccctac acgaccacga atcccatcgg
    33481 taccgaaata tttacgatta ctcatagcgt ttgttttcct ttgcagacag agtggcttcc
    33541 accacccgca tcgcttctac ggtttctttg acgtcatgaa cacgaatgat gtgcgcgcct
    33601 tgcattgcgg caatgaccgc acaggccaga ctaccgctca ggcgctcgga cggccccacg
    33661 ttcagcagct gcccaatcat cgattttcgt gacataccca ccaacagcgg caggttgaaa
    33721 tggtgaaatt cagccaggcg cgccagtaat gaatagttat gggagagatt tttaccgaaa
    33781 ccgaatccgg ggtcgagcaa caatttctct tttgcgatac ccgcctgctc gcaacgtgct
    33841 atttgctcaa taaagtagcg attcacttct gcaaagacat cgtcatactt cggagcttcc
    33901 tgcatggttt ttggatttcc ctgcatatgc atcagacaaa ccggtaaacc ggtttctgca
    33961 gccgcctcca gagcgccagg ttcggaaagg gagcggatat cattaataat gtgagcgcca
    34021 actttcgctg actcacggat gacttctggt ttggatgtat cgaccgagat ccagacttcg
    34081 aagcgttgag caattgcctc aaccacagga ataacacgtt gcaactcttc ttcaacgcta
    34141 acttccgccg cccctgggcg cgtggactcg ccaccaacgt caatgatcgt cgcgccagca
    34201 ttgatcatca gattcgcatg tttcaccgca tctatcagcg agttatgcgt gccaccatcc
    34261 gaaaaggaat caggcgtgac gttgaggatc cccattacgt gaggatggct aaggtccagt
    34321 gaagtaccct gggcaaagag tttcattatg ttatccctgg tatgaattga taagaaaaaa
    34381 ccccggagca cgccccgggg ttttcggtac aaatacagtc atctgatgcg ggaacttact
    34441 tgtcgcctaa ctgctctgac atggtgttac ccgggttcgg cgtacgcggt tcatcaaccg
    34501 gacgaggagc ctttggacta ccattgtcgc cagaattgtt agaagcgcct ggttcttccc
    34561 agcccgctgg cggacgtaca tcgcgacgtg ccatcaggtc atcaatctgc ggtgcgtcga
    34621 tagtctcata tttcatgaga gcatctttca tcgcatgcag aatatccata ttgtcggtca
    34681 gaagctgacg cgcacgatta tagttacgct caatcagtgc tttcacttcc tggtcgatga
    34741 tacgtgcagt ttcatcggac atatgtttcg ctttcgctac gctacggccg aggaatactt
    34801 caccttcttc ttccgcgtac agcagtggac ccaatttctc agagaagccc cactgagtca
    34861 ccatgttacg tgccaggttg gtcgcaactt taatatcgtt ggacgcaccg gtagaaacat
    34921 gttccggccc gtagatgatc tcttctgcca gacgaccacc gtacagcgta gaaatctggc
    34981 tttccagttt ctgacggctg gcgctgattg cgtcgccctc aggcaagaag aaagtcacac
    35041 ccagcgcacg accgcgtggg ataatcgtca ctttatgcac cggatcgtgt tccggcacca
    35101 ggcgaccgat aatcgcatga cccgcttcgt ggtaagccgt tgattctttc tgcgcttccg
    35161 tcatcaccat ggagcgacgt tccgcaccca tcatgatttt gtctttcgct ttctcgaact
    35221 caaccatcga cacaacgcgt ttgttgccac gagcagcgaa cagtgccgct tcgttcacca
    35281 ggttcgccag gtcagcaccg gagaaaccag gagtaccacg agcaatgatt gccgcgtcga
    35341 tatcgggtgc caatggtacg cgacgcatgt gaactttcag gatctgctca cgaccgcgaa
    35401 catctggcaa gccgaccaca acctggcggt cgaaacggcc aggacgcagc agggccgggt
    35461 cgagaacgtc cggacggtta gtcgcggcga taacgatgat accttcgtta ccttcgaagc
    35521 catccatctc aaccagcatc tggttcagag tctgttcacg ttcatcgtga ccaccgccca
    35581 gaccagcgcc acgctggcgg cctacggcgt cgatttcatc gataaagatg atgcacggtg
    35641 ccgctttctt cgcctgttcg aacatgtcac gaacacggga tgcacccaca ccgacgaaca
    35701 tttctacgaa gtcagaaccg gagatagtaa agaacggaac tttcgcttcg cctgcaatcg
    35761 ctttcgccag caacgtttta ccggtacccg gaggaccgac catcaggacg cctttcggga
    35821 tcttaccgcc gagtttctgg aagcggctcg gctcgcgcag atactctacc agttcagcaa
    35881 cttcttcttt tgcttcgtcg cagcccgcaa cgtcagcaaa ggtcgttttg atctgatctt
    35941 ccgtcagcat gcgcgctttg ctcttaccaa acgacatggc acctttgcca ccgccgccct
    36001 gcatttgacg catgaagaag atccagacac caatcagcaa cagcatcggg aaccaggaga
    36061 tgaagataga agccagcagg cttggttctt caggcggttc accgacaacc ttgacgttct
    36121 tggtcaacag gttatccagt aatttcggat cctgaaccgg aatgtaagtg gtataacggt
    36181 tactatcttt cttggtaacg ttgatttcac gtccgttgat acgcgcttca cgaacctggt
    36241 cgttattgac ctcttgtagg aaggtagagt aatccacctt acggccatta gactcgctgg
    36301 gcccaaagct ctggaatact gacatcagca caacggcaat gaccagccag agtattaggt
    36361 ttttcgccat gtcactcaag ggattaacct cttattacaa ctgtgttaaa aacagcgtca
    36421 ggatactcta tatccagcat ctttcaaact ttcgtctgaa atctcccggt tagggtttac
    36481 gcccggtcgc tacaatatac acttctcgcg aacgtgcacg agaagagtcc ggcttacgaa
    36541 ctttgacctt cgtaaacagg gagcgaattt cccttagata ctcatcgaaa ccttcgccct
    36601 ggaacacctt cactacaaaa ctgccacctg gcgctaatac atcacgacac atttctagcg
    36661 ccagttccac cagatacatg gctcggggga tatccaccgc cggtgttccg ctcatgtttg
    36721 gtgccatatc ggacatgaca acctggactt tgctgtcgcc aacgcgctcc agcagtgctt
    36781 tcatcaccag ttcatcacga aaatcgccct gaagaaagtc cacaccaacg ataggatcca
    36841 taggtaaaag atcgcaagcg atgatgcggc ctttgccgcc aatttgggtg accacatatt
    36901 gtgaccaacc acccggagca gcaccaaggt cgacaaccgt cattcccggt ttaaagagtt
    36961 tgtcactttg ctgtatttca tcaagtttaa accaggcacg ggaacgtaat ccctttttct
    37021 gtgcctgttg aacatattta tcgctaaagt gttcctgaag ccagcggctg gagctggcag
    37081 aacgcttctt acctgtcatt taactttccc atggggataa ctcatcgtaa ccaattgcgt
    37141 aaatttttac gcgcctattt ggtgatatat gggagatggc ggtagaatga cccgttttca
    37201 atcccaacgt aagcaaaaaa tacgatgaat ctgagtacta aacaaaaaca gcacctgaaa
    37261 ggtctggcac atccgctcaa gccagttgtt ctgcttggca gtaatggttt gaccgaaggg
    37321 gtgctggccg agattgaaca agcgttagag caccatgaac tcatcaaggt gaaaatcgcc
    37381 accgaagatc gcgaaacaaa aaccttgatc gtggaagcta tcgtgcgcga aaccggcgcc
    37441 tgtaatgtac aggtcatcgg taaaacgctg gtgctttatc gcccaactaa agaacgtaaa
    37501 atctcgctgc cacgctaaga ttatcctaaa gttacacaca tttgctgtgt aaaacgaggg
    37561 gttttccgca ggcaggagag caaaatgcca cgctctgttc gttgataaaa ggccgcagag
    37621 cggccttttt cctttcttta caatacatca acatcttgag tattgggtaa ttcttacagg
    37681 tattccacct taattacttc aaattctact tcgccgcccg gcgttttgat gaccacaaca
    37741 tcatcttctt ctttgccgat caggccacga gcaataggcg agtttacaga aatcaggttt
    37801 tgtttaaagt cagcttcgtc atcgccaacg atgcgataag tctgttcttc gtcagaatcc
    37861 agattcagca ccgttacggt agcaccaaaa ataacgcgcc cattgttggg cattttggtg
    37921 acatcaatca cctgcgcgtt tgacagcttg gcttcgatgt ctttgatacg gccttcgcag
    37981 aagccctgct gttcacgagc tgcgtggtat tcggcgtttt ctttcaggtc gccatgctca
    38041 cgcgcttccg cgatagcagc aatgatttca ggacggcgca cagatttcag aaaatccagc
    38101 tcttcgcgta atttttcagc gccgcgtaag gtcatcggaa tagcttgcat ttgttatacc
    38161 tcttgaatat tcctgatggg gcaagtcttc acccaccccg gctgttcggc ctgcccggca
    38221 taactcctca ccgggaccag aagcaaaaaa ataccgaccc gggtacaagt cccaggtcag
    38281 ctacaattca cattttgatg gtcattttac cctgaagttc ccgaagggtc atcgtttact
    38341 ttatagggcg ttgcgccgta gtatgacggc tcgattccag gttgttagcg cgagattatg
    38401 cgattttcca gatttatcat cggattgacc agctgtatag cgttcagtgt tcaggccgcg
    38461 aatgttgatg agtacgttac tcaactcccc gcaggtgcca accttgccct gatggtgcaa
    38521 aaagtcggtg cgtcggcccc cgctattgat taccacagtc agcagatggc gctgcccgcc
    38581 agtacccaga aagtgattac tgcgctggcg gcgttgattc aactcggccc cgattttcgt
    38641 ttcaccacga cgcttgaaac caaaggcaat gtggaaaacg gcgtacttaa gggtgactta
    38701 gtggcgcgat ttggtgccga tccgacgtta aaacgtcagg atattcgcaa tatggtcgcg
    38761 actttgaaaa aatctggcgt caaccaaatc gatggcaatg tgttgataga tacctccatt
    38821 ttcgccagcc acgataaagc ccccggctgg ccatggaatg acatgacaca atgctttagc
    38881 gctccgcctg ctgccgccat agttgaccgc aactgtttct ccgtctcgct ctacagtgcc
    38941 ccaaagcctg gtgatatggc ttttatacgc gtggcatctt attaccccgt tacgatgttc
    39001 agccaggtac gcaccctccc ccgtggttct gccgaagcgc aatactgcga actggatgtg
    39061 gtgccaagcg acctgaaccg ctttacgctg acgggatgcc tgccacaacg ttctgagccg
    39121 ctcccgttgg cttttgccgt gcaggatgga gccagctatg ccggtgcaat tctgaaagat
    39181 gagttaaaac aggcgggtat cacctggagc ggaacactgc tgcgccagac tcaggttaac
    39241 gaacctggaa cggtagttgc cagtaaacag tcggccccgc tgcacgatct gcttaagatt
    39301 atgctgaaaa agtcggacaa catgatcgcc gatacggttt tccgcatgat cggccatgcg
    39361 cgcttcaatg tgcctggaac atggcgggct gggtcggacg ccgtgcgtca gatcctgcgc
    39421 cagcaagccg gtgtcgatat tggtaacacc attattgccg atggttcagg gctttcgcgg
    39481 cataacctga ttgcccccgc caccatgatg caggtgctgc aatacattgc ccaacacgac
    39541 aatgaactta actttatctc catgctgccg cttgcgggct atgacggctc tttgcagtac
    39601 cgtgcaggtc tgcatcaggc gggcgtggat ggcaaagtct cagcgaaaac cggttcgttg
    39661 cagggggtat ataacctggc gggattcatt accacagcga gcgggcaacg aatggcattt
    39721 gtacaatatc tttctggcta tgcagtaaaa cccgcggatc agcgtaatcg ccgtattccg
    39781 ttagtgcgtt ttgaaagccg tttgtacaaa gatatttatc agaacaatta gtcaaaaaga
    39841 aaccccggcg catggccggg gtttcagatt attgacaaag tgcgctttgt ttatgccgga
    39901 tgcggcgtga acgccttatc cggcctacaa aatcgtgcaa attcaatata ttgcaatcat
    39961 cttgtaggcc tgataagtgt agcgcatcag gcttatttgg cattggtcat tagtgattaa
    40021 cgcttgtaaa tgaactcaac gccttcttcg tcgtcttcgt cccagtcgtc atcccagtct
    40081 tcatcatcct cttcagcaat ctcttcaagc tgctgacggt gataatcatc ccacatgaat
    40141 tcgactttct ctggctgttt cgcttcttca gcctgcacga ccgggttttc aatgataaag
    40201 gtcatcacat cccagcagag atctttcacg cccagtccgc tcgccgcaga gatcagataa
    40261 tatttatctt cccagcccag cgcttcagcg atcgctttcg ctttctcttc ggcttctgcc
    40321 ttatccagca gatcgatctt gttgaagact aaccaacgcg gtttcgccgc cagatcctgg
    40381 ctgtattttt ccagctcgct gataataata cgcgcgtttt caaccggatc ggtgccgtca
    40441 atcggatcga tatcgatgag gtgcaacagg acgcggcaac gttccaggtg cttcaggaag
    40501 cgaatgccca gacctgcgcc ttccgcagcg ccttcaatca gtcctggaat atcggcaaca
    40561 acgaagctct tttcgttgtc cattcgtacc acacccagac ttggcaccag agtggtaaac
    40621 ggataatccg ccactttcgg tttagccgcc gataccgcac gaataaaggt cgatttaccc
    40681 gcgtttggca tccccaacat accgacgtca gccagcagca tcagctccag cagcagctca
    40741 cgcttatcgc ccggcgtacc gttagttttc tgccgtggtg tacggttaac tgacgatttg
    40801 aaacgggtat tgcccagacc gtgccagccg cccttagcaa ccagcagacg ctgaccgtgt
    40861 ttagtcatat cacccatggt ttcaccagta ccctggtcga ttacacgcgt acctaccggc
    40921 actttaatcg tcacgtcttt accgcgctta ccggtacaat cgcggcttgc gccgttctga
    40981 ccgcgctctg cacggaaaga tttttcaaaa cgataatcga taagcgtgtt caggttctcg
    41041 tcggcttcca tccatacgtc accaccatca ccgccgtcgc cgccatccgg gccgcctttc
    41101 ggaatatact tttcgcggcg gaagctcacg caaccattac cgccatcacc tgcaacgacc
    41161 agaatcgatg cttcatcaac aaacttcatt ttattctccg taaatcattc gcctgcgcgg
    41221 ggttgcgata ccaccgttgt atgcttacgt aatccgcccc aaatacgatg accaatggcg
    41281 gaatacatcg cgcccgcaac cacgacaaac gcaccgagat aacctaaaag gtttaacatc
    41341 ggtctggcga agaaatcggg ccaggccagt gataaaagat ctgaaaaaaa cagcgtaaac
    41401 agtggggtga gcgtgatgat cgcgctcacc tgcgctgcct gccagcgagc catcgcttcc
    41461 gccaaggcgc catatcctac caaggtattc agtccgcaaa aaattaaaca tgcgagctgc
    41521 cagtggctaa gctgcgctat cactccaggc tttgccagag ggaagagcgc gattgtacat
    41581 aaagtgtaca gtaaaaacag gatctgcggt gaggccagcc gacgcaataa aaccttttgc
    41641 gccacgccat aactcaccca aaccgtcgcc gcaccgaccc caaagataac tccccaggtg
    41701 taatcggtga gctttgtaaa tatctcgacc agactggtgt taaaaaacat caccagaccg
    41761 ctcaggagca tcaatgcccc tacaacctga gtgctgcgca ttttctcttt caggataaat
    41821 acgctggcaa ccatcatgcc aactggcgag agttgcccaa tcacctgcga agcggtcgga
    41881 ctcaggtatt gcaaggatga gctgaacagg atgaagttcc caaacagccc ggcggtcgcc
    41941 actgccaaca aaatcaacca gcgtggctta cgaaacacgc gtaatggcgg caacctcttc
    42001 ttcaccgcaa gaatggcacc aaggccaata ctcgccatca agaaacggta aaacacgatt
    42061 gtcggaggtt ccatcacctc cagcacctgc ttcattgcga ttggcaacgc cccccagcaa
    42121 attgctgtgg taagcgccaa aagaatgcca atgcctgcct gctgcttcat accgtattcc
    42181 ctgcaaagcg catttgcgga ttaacgtcgc accatgcgca tttttaccgg ttatcgaatg
    42241 taaaaagccc cgcaacgtgt tgcggggctt tcatccgtta ccgggacgcg aaaaacttat
    42301 tcagcttcga tgctgataaa tttacggttt ttcgggcctt taacttcgaa tttcacttta
    42361 ccgtctgctt tagcaaacag agtgtggtca cgaccgcaac ctacgttagc gccagcgtgg
    42421 aatttggtac cacgttgacg aacgatgatg ctacccgcca gaacggattc gccaccgaaa
    42481 cgcttaacgc ccaagcgttt agcttctgaa tcgcgaccgt tacgtgtgga gccgccagcc
    42541 tttttatgtg ccatttgaaa tctctcctca ggtcttaggc gctgatgcca gtaattttca
    42601 catcagtgaa ccactgacga tggccctgct gcttacgata gtgtttacga cgacgaaact
    42661 taacgatttt aactttctcg ccacgaccgt gagcaacaac ttcagctttg attacgccgc
    42721 catcaacgaa aggaacgccg attttgactt cttcaccgtt tgcgatcatc agcacttcag
    42781 cgaactcaac agtttcgcca gttgcgatgt ccagcttttc caggcgaacg gtctgacctt
    42841 cgcttactcg gtgttgttta ccaccacttt ggaaaaccgc gtacatataa aactccgctt
    42901 ccgcgcacac cttttcaatg attcagagtg cgctataaat attcacaata gggcgcgaat
    42961 attacgcaaa acgcacgcct ttgacaagtg ctacagtcaa tacacgaaga aaaaaacaca
    43021 acttgtacgg taacgtttat ctgtgccatt ttttcagtac aattacccta tattcctaac
    43081 cataaaccct aagttgcctt tgttcacagt aaggtaatcg gggcgaaaag cccggctttt
    43141 gcgatgaatt tagaaaaaat caatgagtta accgcgcaag atatggcggg tgttaatgcg
    43201 gcaatccttg agcagcttaa ttccgacgtc caactgatca atcagttagg ctattacatc
    43261 gtcagcggcg gcggtaaacg tattcgtccg atgattgctg tactggctgc acgagctgtt
    43321 ggctatgagg gaaatgcgca tgtcaccatt gctgccctga tcgagtttat ccacacggcg
    43381 actctgctac acgacgacgt tgtggatgaa tcagatatgc gcaggggtaa agctaccgcc
    43441 aacgccgcat ttggcaatgc cgccagcgtg ctggtaggcg attttattta tacccgcgcg
    43501 ttccagatga tgaccagcct cggttcactc aaagtgctgg aagtcatgtc agaagctgta
    43561 aacgtcatcg cagaaggtga agttctgcaa ctgatgaacg ttaacgatcc ggacatcact
    43621 gaagaaaact acatgcgcgt tatctatagc aaaaccgcgc gtctgtttga ggctgccgcg
    43681 cagtgttccg ggattctggc tggctgtacg ccggaggagg agaaaggcct gcaggattat
    43741 gggcgctatc tcggcactgc tttccagttg atcgacgatt tactcgatta caatgccgat
    43801 ggcgaacagt taggtaaaaa cgtcggcgac gatctgaacg aaggtaaacc gacgctgccg
    43861 ctgctgcatg cgatgcatca tggcacacca gaacaggcac agatgatccg taccgccatc
    43921 gaacaaggta acggtcgcca tcttctggaa ccggttctgg aagcaatgaa cgcttgtgga
    43981 tctcttgaat ggacgcgtca gcgcgccgag gaagaagcag acaaagccat cgcagcgtta
    44041 caggtgctcc cggacacccc ttggcgagaa gcactcatcg gcctcgcgca catcgctgtt
    44101 caacgcgatc gttaatcccc tcccttcatc ccgcgcagtg cgcgggatga ttccagtaaa
    44161 ttccataaaa acactaattc agcgctataa cacctgaaaa aagggcgatg aatctttata
    44221 tattctgaat attcacactc tttacaggaa ctttttagcg caatagacca tcaggagtat
    44281 agtgatgccc gacagaagaa gtgttctgaa tgaaagcgac taacttaagg agtgaggaaa
    44341 gatggaaagt aatttcattg actggcatcc cgctgacatc attgcaggtt tgcgcaagaa
    44401 aggaacgtca atggcggcgg aatctcgcag aaatggtttg agttcctcaa cgctggcaaa
    44461 cgcattatcg cgcccatggc cgaaaggaga gatgattatt gcgaaagccc tgggaactga
    44521 cccctgggtt atctggccat cacgctacca tgatccgcag acccatgagt ttatcgacag
    44581 aacgcagttg atgcgtagct acactaaacc gaaaaaatga gtggtctggc ggtagccccg
    44641 cgaacggggc tgccagctct cagacgatta ctcgcctttc acacgctcaa tatttgcacc
    44701 taaagcgcgc agtttgtctt caatgcgttc gtagccacga tcgatgtgat aaatacgatc
    44761 aaccaccgtc gtcccttccg caatacagcc agccagcacc aggcttgctg atgcacgcag
    44821 atcggttgcc ataacctgtg caccagaaag tttttcaaca ccatgacaaa taacggtatt
    44881 gctttcgatt tcggcgtgcg cgcccatacg gctcagctct ggcacatgca taaagcggtt
    44941 ttcaaagacc gtttcggtga tgaacccggt cccttcagcc accaggttca acagcgtgaa
    45001 ctgggcctgc atatcggtcg ggaatgccgg atgcggcgcg gtacgtacgt taacagcctt
    45061 cggacgtttg ccatgcatat ccaggctaat ccagtcttcg ccgacttcga tgtccgctcc
    45121 agcatcacgc agtttcgcca gcacggcgtc cagagtatct ggctgcgcgt tacggcagat
    45181 aattttgcca cgagagatcg ccgccgccac caggaaagta ccggtttcga tacgatccgg
    45241 cagcacgcga tagacaccgc cgcctaaacg ttccacacct tcgatgacga tacgatcggt
    45301 gccctgaccg ctaattttcg cacccagcgt aatcaggaag ttcgcggtat cgacgatttc
    45361 cggttcacgc gctgcgtttt caataatcgt ggtgccttcc gccagggttg cagcacacat
    45421 gatggtcacc gttgcgccaa cgctgacttt atccatcacg atatgtgcgc ctttcagacg
    45481 accatcgacg gaagctttaa cgtaaccttc ttccagtttg atggtcgcgc ctaattgttc
    45541 gaggccagaa atgtgtagat caaccggacg cgcaccgatc gtacaaccgc caggtagtga
    45601 aacttgcccc tgaccaaagc gcgctaccag cggccccagc gcccagatag aagcacgcat
    45661 ggttttaacc agatcgtaag gtgcgcagaa tacattaacg tcgcgggcat caatatgcac
    45721 agaaccatta cgttctactt tcgcacccag ctggcttagc agtttcattg atgtatcgac
    45781 gtctttcagt ttcgggacgt tctggatctc taccggttct tccgccagta gtgcggcaaa
    45841 aagaataggc agagcagcat ttttagcgcc ggaaattgtg acttcgccct ggagcttcgt
    45901 tggcccctga acacgaaatt tatccattta gtttgttctc agttaacaat tcatatccgc
    45961 taccggcgaa tcgcccatag ctcaaaagcc gttcagtttg cgatcgcgcg cccactccgc
    46021 aggggtatac gctttgatcg acacagcatg aatgcggtta tccgcaatat attccatcag
    46081 cgggccatag accgtctgct gttttttaac ccgactcata ccgtcaaaca actcacccac
    46141 ggcaataacc tgaaagtggc tgccatcgcc ggaaacgtgg acttcctgga gggagagagc
    46201 gttcatcaac acgctctgaa tttcattatt ttccatggga tcttcaatca tcagttaata
    46261 aaccagcgaa acatcttaga gcaaagttgc gctggcataa ataagcaaaa agcctcgctg
    46321 ataaatcaga caaggctcga cttgcaggca ggtttgccgg acaggcggtt aacgccatat
    46381 ccggcctgaa aaaatttaac gaggcagaac atcagcaggc aaattataca atttcgcgag
    46441 ggtatacact ttgtcgttta ccccctgaag cgtcacgttg ttgccctgct ttttcgccag
    46501 atcgataaga tggagcagca gtgccagtcc ccccgtatcc acgcgggaca cacggctaag
    46561 atcgatgcag gtaatcccct tcaccgcctc ctcacgcatt tcccaaagcg gtagcagaac
    46621 gtcctgatcc agctctccgg ataacgccag cgtgtcaccc gtctgcatcc agctcagtga
    46681 ctcgctcatt attttttctc ttccagagtg attttctgtt gagaaatcga tttcagttgc
    46741 gcagtcaggc cgtcgatacc tttggtacgc agcagcgttc cccactcgtt ttgtttggtg
    46801 gtgatcatac tgacgccttc agcaatcatg tcgtaagctt gccagtttcc cgtctgggag
    46861 tttttacgcc actggaagtc cagacgcacc ggcggacggc cattcgggtc aataatggta
    46921 acgcgaatag gcacaatggt tttatcgccc agcggctgtt ctggcgcaat ctgataggtt
    46981 tgaccgtgat acatcgccag cgcctgaccg taagcctgct tcaggtactc acggaaagcg
    47041 gcaaagtagg cttcacgttg agcaggggtc gcactcttgt aatactggcc cagcaccagc
    47101 gcaccggcgt atttcacctg tacgtatggc agcagttcct gatcaacaat ggtacgcaga
    47161 taatccgggt tggcccgaat ttgcggttgc tcattcttca ggcgatcgaa cgttttctgc
    47221 gccgcctcgt ccatcagctt ataaggattg gtctggtctg ccgcggttgc cgcactcaga
    47281 ggtgcaatca ccagcaaagc gaccatcatt aaacgtttaa acatgcgtcg gttctcctga
    47341 aattatttcg ttgtacccac aggttcagtg gtttcattat tacctggcgc agcagctggc
    47401 gcatcgccac tattcttatt gtcatcgcct ttactaccgt aaaggaactg accaatgaga
    47461 tcttccagca ccatcgcaga cttggtgtcc tgaattgtat cgccatcctt caggatagca
    47521 gtccccagtt ccgggtcttc aaaaccgacg tttaatgcca gatattgttc tcccagcagg
    47581 ccggaagtac gaatgctcag cgaactggta tcaggaatgt ggttataacg ttgttcaatt
    47641 tccagcgtta cgcgcggcag ataggttttc gggtccagcg taatatccgc cacccggccc
    47701 acaacaacac caccaatgct gaccggagag cgcgctttca ggccgccaat gttatcgaac
    47761 gtcgcataaa gcgtgtaggt cgattcagta cgtatggacg tcacgttcgc cgccttcagg
    47821 caaacaaaca gcgccgccag cagtgctgct aataaaaaga tacccaccca aatttcattt
    47881 tttttcgttt gcatgaactc aattcccaaa catcaatgcg gtcagcacaa aatccagccc
    47941 cagaacagcc agagacgagt ggacaacggt gcgagtggtt gcccggctaa tcccggcaga
    48001 cgtcgggatg gcgtcgtagc cgttaaacaa cgaaatccac gtcaccgtga tggcgaacac
    48061 cacgctctta atcagacagt tgaccagatc catacgccag tcgacggcat tttgcattgc
    48121 cgaccagaag aacccgctat caatgccttt ccaactgacg ccgaccagcg atccgcccca
    48181 gatccccacg gcgacgaaga taaccgtcaa cagtggtaat gaaataaccc cagcccagaa
    48241 acggggagaa ataacccgac gcagcggatc caccgccatc atctccatac tggagagttg
    48301 ctctgtagcg cgcatcaggc cgatttctgc ggttaacgcc gaaccagcac gcccggcaaa
    48361 caacagcgcg gcaaccaccg gccccagttc acgcagtagc gataacgcca ccagcatacc
    48421 cagactggtt tccgcgctat aagtggtcag aaccagataa ccttgcagcc ccaacaccat
    48481 tccgatgaac acgccagaaa ctacaataat cagcatcgac aggacgccga cattatagag
    48541 ctggcgcacc agcagcggcg catgtttgcg aaattccggt ttgccgacca gcgcattgaa
    48601 taacattaac ccggcccgcc cgaacgttct cagggtttta atccctttat gtccgagcga
    48661 cgccagcgca tttaacagca tgagtggctt aactccctgg taaaagatca gcgtgataat
    48721 cgccggcagg atagcggaac ggaacaggcc cgtcagctat cccgtccaga aactgacgta
    48781 cgcgcggatc aggattcgtc tgcaacgcct gggcactgcc atgagcgaca atttttttgt
    48841 ccgccaggat ccaggcgtga tccgcaatac ttaacacttc cggcacatcg tgagaaacca
    48901 ccacacaagt cacgcccagc gcgctgttca gctcagaaat cagcttcacc agtacgccca
    48961 tggtgatggg atcttgccca acaaaaggtt catcaaacat gatgagatcc ggctccagcg
    49021 caatcgcacg cgccagcgct gcacgccgcg ccatcccacc ggaaagttca gaaggcatta
    49081 gtttagccgc tccacgcagc cccacggcct ccagcttcat catcaccgta ctatgcaaca
    49141 atggcgcggg aagttgggta tgttcgcgca gtggataggc gacgttgtca aatacgttca
    49201 tatcagtgaa caacgccccg gactgaaata acatgctcat ccgtttacgc actgtataca
    49261 ggcgcgaacg agacatcgcc ggaatattct caccatcgaa aaggatctca ccatgatctg
    49321 gtgcgatttg cccgccaatc agacggagta gcgtcgtttt accgatgccc gatggcccca
    49381 tgatcgccgt gatcttccct cgcggcacgg tcagggaaat attatcgaag atgcagcgat
    49441 tgccacgcgt aaaactgaca tcgcgcatat cgactaaatt cgccacagac tgctccataa
    49501 ttcacccttc gtcttgcgtt gattttctaa gcatggcgct caatttaacc ttgaacccaa
    49561 catatttaca gaatattacc cgccgtggtt agcgaaagct ggcatttgtt ttacttttta
    49621 gccgcataaa gtcaaaatta agaatccgtt acggctttct gaaaatcttc agcggaccgg
    49681 cgagtatacc tgaagaaagg acgttagatg cttttagcta cggcactgtt aattgttggt
    49741 ttacttttgg tcgtttacag tgccgaccgc ctggtttttg ccgcgtctat tctttgccga
    49801 acctttggca tcccgccgct gatcatcggc atgacggtgg tcagtattgg tacatcgtta
    49861 ccagaaatca tcgtctcgct tgccgcgtct ctgcacgaac aacgcgattt agccgttggt
    49921 acagccctcg gctcaaacat tatcaatata ttgctgatcc tcggtctggc tgcgctggtt
    49981 cgtcctttta ccgtccattc cgatgttctg cgccgtgaat tacccttaat gttgttggtc
    50041 agcgtggtgg ccggttccgt actctatgac ggacaactta gtcgaagcga tggtatcttt
    50101 ctcctctttc tggctgtgct atggctgctg ttcattgtta aacttgcacg tcaggggact
    50161 gacagcctga ccagagagca gcttgcagag ctgccgcgtg acggcggatt gcccgtcgcg
    50221 tttttatggc tcggcattgc gcttatcatc atgccagtgg ccacgcggat ggtggttgat
    50281 aacgccacgg tgctggcgaa ttactttgcc atcagcgagt tgacgatggc tctgacggca
    50341 attgctatcg gaaccagtct gccggaactg gcaaccgcaa tagcgggggt tcgcaaaggt
    50401 gaaaacgaca ttgctgtcgg aaatatcatt ggcgcaaaca tttttaatat tgtcatcgtg
    50461 ttgggtttac ccgcgctgat aacgccagga gagattgatc cactggcgta cagtcgtgac
    50521 tacagcgtga tgttgctggt gagcattatt tttgcgttgc tgtgctggcg gcgctccccg
    50581 caaccgggcc gtggtgtagg ggtattatta actggcggat ttatcgtatg gctggcgatg
    50641 ttgtactggt tatcgccaat actcgttgaa taactggaaa cgcattatgt cgcacgtaga
    50701 gttacaaccg ggttttgact ttcagcaagc aggtaaagaa gtcctggcga ttgaacgtga
    50761 atgcctggcg gagcttgatc aatacatcaa tcagaatttc acgcttgcct gtgaaaagat
    50821 gttctggtgt aaagggaaag ttgtcgtcat ggggatgggg aaatcggggc acatcgggcg
    50881 caaaatggcc gcaacgtttg ccagcaccgg tacaccttca tttttcgtcc atcctagtga
    50941 agccgcgcat ggtgatttag gcatggtcac cccacaggat gtggtgattg ctatctctaa
    51001 ctcaggtgaa tccagcgaaa tcacggcctt aattccagtg cttaagcgtc ttcacgtacc
    51061 gttaatctgc atcaccggtc gcccggagag cagcatggcg cgcgccgcag atgtgcatct
    51121 gtgtgttaaa gtagcgaaag aagcctgtcc gttagggctg gcaccgacca gcagcaccac
    51181 cgccacgctg gttatgggcg atgccctcgc tgtcgcgctg ttaaaagcac gcggctttac
    51241 tgctgaagat tttgcgctct cacacccagg cggcgcactg ggtcgtaaac ttctgctgcg
    51301 cgtaaacgat attatgcata cgggcgatga gatcccgcat gttaagaaaa cggccagtct
    51361 gcgtgacgca ttgctggaag ttacccgcaa aaatcttggt atgactgtca tttgcgatga
    51421 caatatgatg attgaaggca tctttaccga cggtgattta cgccgtgtct tcgatatggg
    51481 cgtggatgtt cgtcagttaa gtattgccga tgtgatgacg ccggggggaa tacgtgtgcg
    51541 ccctggcatt ctggccgttg aggcactgaa cttaatgcag tcccgccata tcacctccgt
    51601 gatggttgcc gatggcgacc atttactcgg tgtgttacat atgcatgatt tactgcgtgc
    51661 aggcgtagtg taaagattca aggataaaca acaatgagca aagcaggtgc gtcgcttgcg
    51721 acctgttacg gccctgtcag cgccgacgtt atggcaaaag cagagaacat tcgtctgctg
    51781 atcctcgacg ttgatggcgt actgtcagat ggcctgattt atatgggcaa taacggcgaa
    51841 gagctgaaag cgttcaatgt tcgtgacggt tatggcattc gttgtgcgct cacctctgat
    51901 attgaagtcg ctatcattac cgggcgaaag gctaaactgg tagaagatcg ttgtgccaca
    51961 ttggggatca ctcacttgta tcaggggcag tcaaacaaac tgatcgcctt tagcgatttg
    52021 ctggaaaaat tggcgattgc cccggaaaat gtggcttatg ttggcgatga tctcatcgac
    52081 tggccggtaa tggaaaaagt gggtttaagc gtcgccgtgg ccgatgcgca tccactgttg
    52141 atcccgcgcg ccgattacgt gacgcgcatt gctggcggtc gtggcgcagt gcgcgaagtt
    52201 tgcgacttat tactcctggc gcagggcaaa ctggatgaag ccaaagggca atcgatatga
    52261 gtaaagccag acgttgggtt atcattgtgc tatcactggc ggttctggtg atgatcggca
    52321 ttaatatggc cgaaaaagac gataccgccc aggtggtcgt caacaacaat gatcccacct
    52381 ataaaagcga gcatacggac acgctcgtct ataacccaga aggggcacta agctatcgat
    52441 tgattgctca acacgttgaa tattattccg atcaggccgt ttcgtggttt acgcagccgg
    52501 tacttaccac gtttgataag gataaaatcc cgacatggtc cgtaaaagca gataaagcca
    52561 agctgaccaa tgaccggatg ctctatttat atggacacgt tgaagtcaac gcacttgtgc
    52621 cagactctca acttcgcaga atcacgacgg ataacgcgca gatcaatctg gtgacgcagg
    52681 atgttacctc tgaagacctc gtcacgttat acggaacaac atttaactcc agcggtctga
    52741 aaatgcgcgg caacttacgc agcaagaacg ccgagctgat tgaaaaggtt agaacatcct
    52801 atgaaattca aaacaaacaa actcagcctt aatcttgtgc ttgccagctc acttctggcc
    52861 gccagcattc cggcatttgc cgtaaccgga gacactgatc agccgatcca cattgaatcg
    52921 gaccagcaat ctcttgatat gcaaggcaac gtggttacct ttaccggtaa tgtcatcgtc
    52981 acccagggca ccatcaaaat taatgccgac aaagtggtcg ttacccgtcc gggcggcgaa
    53041 caaggtaaag aagtgattga cggatacggt aaaccggcaa cgttctacca gatgcaggac
    53101 aacggtaaac ccgttgaagg tcacgcttcc cagatgcact acgaactggc aaaagatttt
    53161 gtcgttctga cgggtaatgc ttatctgcag caggtcgata gcaacattaa gggcgataag
    53221 atcacttacc tggtgaaaga gcagaaaatg caggctttca gcgacaaagg caagcgcgta
    53281 accaccgttc tggtgccgtc gcagctgcag gacaaaaaca acaaaggcca gaccccggca
    53341 cagaagaagg gtaattaatt cgttatggca acattaactg caaagaacct tgcaaaagcc
    53401 tataaaggcc gtcgcgtggt agaagacgtc agcctgaccg tcaactccgg ggaaattgtc
    53461 ggtctgctgg ggccaaacgg tgccggtaag accaccactt tctacatggt tgtaggcatt
    53521 gtgccgcgcg atgcgggcaa catcattatt gatgatgacg atatcagtct gctgcctctg
    53581 catgcacgcg cgcgccgcgg tatcggctat ctgccacagg aagcctccat tttccgtcgc
    53641 ctcagcgttt acgataacct gatggcggta ctgcaaattc gtgacgactt gtctgctgaa
    53701 caacgtgaag accgcgcgaa cgagctgatg gaagagtttc acattgagca cctgcgtgac
    53761 agcatggggc agtcactctc cgggggtgaa cgtcgccgtg tagaaattgc ccgcgcactg
    53821 gctgcgaatc cgaaatttat tctgctcgac gaaccgtttg ccggggttga cccgatctcg
    53881 gttatcgaca ttaaacgcat cattgaacac ctgcgcgaca gcggcctggg cgtgctgatc
    53941 actgaccaca acgtgcgtga aacactggcg gtttgtgaac gcgcttatat cgtcagtcag
    54001 gggcatttga tcgcccacgg cacgcctaca gaaatcttac aagacgaaca cgttaagcgt
    54061 gtataccttg gggaagactt cagactctga tagggtagaa gtttgcgacg ttttagcagg
    54121 agagtacgat tctgaacatg aagcaaggtt tgcaactcag gcttagccaa caactggcga
    54181 tgacgccaca gctccaacag gcaattcgtc tgttgcagtt gtcgacgctg gaacttcagc
    54241 aggagctaca gcaggcgctg gagagtaatc cgctgcttga gcaaatcgac actcatgaag
    54301 aaatcgacac ccgcgaaacg caagacagtg aaacgctgga caccgccgac gcgctcgaac
    54361 aaaaagagat gccggaagag ctgccgctcg atgccagttg ggacaccatt tacaccgctg
    54421 gtacaccatc cggcaccagc ggtgactaca ttgacgacga gctgccggtc taccagggcg
    54481 aaacgacgca gaccttgcag gattacctga tgtggcaggt cgagctgaca ccgttttccg
    54541 acactgaccg cgctattgct acctctatcg tcgatgccgt tgatgaaacc ggttatctga
    54601 ctgtcccgct ggaagatatt ctcgaaagta tgggcgatga agagatcgac atcgacgagg
    54661 ttgaagccgt ccttaagcgg atccaacggt ttgacccggt cggtgtagcg gcaaaagatc
    54721 tgcgtgactg cctgctaatc caactctccc aattcgataa gaccacgccg tggctggaag
    54781 aggccagatt gatcattagc gatcatctcg atctgttagc caatcacgac ttccgcactt
    54841 taatgcgcgt cacgcgtctg aaagaagatg tgctgaaaga agccgtcaat ctgatccagt
    54901 cgctcgatcc gcgccccggg cagtcgatcc agactggcga acctgagtat gtcattccag
    54961 atgtgctggt gcgtaagcat aacggtcact ggacggtaga actcaacagt gacagcattc
    55021 cgcgtctgca aatcaaccag cactacgcct cgatgtgcaa taacgcgcgc aacgatggtg
    55081 acagccagtt tatccgcagc aatctgcagg atgccaaatg gttgatcaag agtctggaaa
    55141 gccgtaacga tacgctactg cgcgtgagtc gctgtatcgt tgaacagcag caagccttct
    55201 ttgagcaagg tgaagaatat atgaaaccga tggtactggc cgatatcgcc caggctgtcg
    55261 aaatgcatga atcgacgata tctcgcgtga ccacgcaaaa atacctgcat agtccacgag
    55321 gcatttttga actgaagtat ttcttttcca gtcacgtcaa taccgagggc ggcggcgaag
    55381 cttcctccac ggcgattcgt gcactggtga agaaattaat cgcggcggaa aacccagcga
    55441 aaccgttgag cgacagcaag ttaacctctt tgctgtcgga acaaggtatc atggtggcac
    55501 gccgcactgt tgcgaagtac cgagagtctt tatccattcc gccgtcaaac cagcgtaaac
    55561 agctcgtttg acccaaccga taaggaagac actatgcagc tcaacattac cggaaataac
    55621 gtcgagatca ccgaggcact gcgcgaattt gttacagcta aatttgccaa acttgagcaa
    55681 tattttgacc gaatcaacca ggtctatgtt gttctgaaag tggagaaagt cacccacacc
    55741 tcagatgcaa cactgcatgt aaacggtggt gaaattcatg ccagcgcgga aggtcaggac
    55801 atgtacgctg ccattgatgg cttaattgat aagctggcac gccagttgac caaacataaa
    55861 gataaactga aacaacacta attgtccggg caattagcat gtgcatggcg gcctgttgtg
    55921 cggcacaacg ggccatttgt acggttaatg ctccgagcct gttccactgt ttgagtgggc
    55981 aggttcttag gtgaaattat gacaaataat gatacaactc tacagcttag cagtgttctt
    56041 aacagggaat gtacgcgaag ccgcgtccac tgtcagagca aaaaacgcgc cctggaaatc
    56101 atcagcgagc tggcggcgaa acaacttagc ctgccgcctc aggtggtttt tgaagctatc
    56161 ctgacgcggg aaaaaatggg cagtaccggt atcggcaatg gtattgccat tccgcatggc
    56221 aaacttgaag aagatactct gcgtgccgtt ggcgttttcg ttcagctcga aacgcctata
    56281 gctttcgatg ccatcgacaa ccaaccggtg gacttacttt ttgccctgct ggtgccggca
    56341 gaccaaacta aaacgcacct gcatactctg tcgctggtgg cgaaacgtct ggcggacaaa
    56401 accatctgcc gccgtttacg cgcagcccag agcgatgaag agctgtatca aatcattacg
    56461 gataccgaag gtactccgga tgaagcgtag ttattcggta atgtctcttt tagacgttgt
    56521 gaggagaaac agtacatggt actgatgatc gtcagcggac gttcaggttc aggtaaatct
    56581 gtcgccctgc gtgcgctgga agatatgggt ttttactgcg tggataacct tcccgtagtg
    56641 ttgttacccg atctggctcg aactctggcc gatcgcgaga tttctgccgc cgtcagcatt
    56701 gatgttcgta atatgccgga gtcaccagaa atattcgaac aggcgatgag taacctgcct
    56761 gacgctttct caccgcaact actgttcctg gatgccgacc gtaatacctt aattcgtcgt
    56821 tacagtgaca cgcgccgact gcatccgctt tccagcaaaa acctgtcgct ggaaagtgct
    56881 atcgacaaag aaagcgattt gctggagcct ctgcgttcgc gagcggatct gattgtcgat
    56941 acctcagaaa tgtccgttca cgagctggct gaaatgctgc gtacccgtct gctgggtaaa
    57001 cgtgaacgcg aactgaccat ggtctttgag tctttcggct tcaaacacgg tatccctatc
    57061 gatgcagatt acgtctttga cgtgcgcttc ttgccgaacc cgcactggga tccgaaactg
    57121 cgtccaatga caggtcttga taaacctgtc gccgcgttcc tcgaccgcca cacagaagta
    57181 cacaatttta tctaccagac gcgaagctat cttgagctat ggttacctat gctggaaacc
    57241 aacaaccgta gctacctgac ggtcgccatt ggttgtaccg gcgggaagca ccgttcggtg
    57301 tatattgcag agcaactggc agactacttc cgctcgcgcg gtaaaaacgt ccagtcacgc
    57361 catcgtacgc tggaaaaacg taaaccatga ccgtcaagca aactgttgaa atcacaaaca
    57421 agctgggcat gcatgcccgg cctgcaatga agctgtttga attaatgcag ggttttgacg
    57481 ctgaagtgct gttacgtaat gacgaaggca ccgaggctga agccaacagc gttattgcgc
    57541 tgctgatgtt ggattctgcc aaaggacggc agattgaagt tgaagcgacc ggtccacagg
    57601 aagaggaagc actggccgcc gttatcgccc tctttaattc tggttttgat gaagattaat
    57661 cttcatcact ttgacataca actcccttca agctcccccg acaataagaa aatcacgtac
    57721 tgaaatcgtt ctcaatcaac gtcatttgta cattttgtgc gcttttcact ttcagaagaa
    57781 ccttaagaaa accttaagag gcattgttta ggttttgttt aagttaatcg accatactgg
    57841 agatcgtcag aaaatatttc caggagatgg catgattcgc ttatctgaac aaagtcccct
    57901 gggcaccgga agacatcgga aatgttatgc gcatccggaa gatgcccaac gctgtatcaa
    57961 gattgtctac catcgtggcg atggtggcga taaagagatc cgccgtgagt taaagtacta
    58021 cgcgcatctt ggtcgccggt taaaagactg gagtggaata ccgcgctatc acggtaccgt
    58081 tgagacggat tgcggaaccg gttatgtcta cgatgtgatt gccgattttg acggcaagcc
    58141 ttccattacg ctgaccgaat ttgctgaaca atgccgttac gaagaagaca ttgcacaact
    58201 gcgccagcta ctgaaacagc tgaagcgtta tttgcaggat aaccgtatcg tgacgatgtc
    58261 gctgaagccg cagaatatcc tctgtcaccg catcagtgag tcagaggtta tcccggtcgt
    58321 ctgcgacaac attggtgaaa gcacattgat tccactggcg acctggtcga agtggtgttg
    58381 tctgcgtaag caagagcggt tatggaaacg atttatcgcc caaccggcgc tggctattgc
    58441 cctgcagaaa gatctgcaac ctcgtgagag caaaacgctg gccctcactt cgcgcgaagc
    58501 ttaatccagc tggtgttgtt gcataaacgg ctcaccgcct aactgataca tctgccgtaa
    58561 aatccacgcc tgacggctac gcacgtagcc cgatggcgag gagactttga aacgaagtgg
    58621 attaggtaat acagctgcca gtaatgcagc ttccgaccgg gtaagtttgc tcgcgggttt
    58681 gtggaaataa cgttgtgccg cagcttcgac gccaaacaca ccgtcgccaa attcggcgat
    58741 attcaggtaa accgtcagga tacgcttttt gctccagact gtttctatcc ccagcgttaa
    58801 tccagcttcc agcccttttc gtacccagct acgcccatcc cataaaaaga gattttggcc
    58861 gtctgctgag aaatcgttga agcaccgcga atacggtttt cattgcgctc gttgtgcgcc
    58921 agggctttct caatggaagc gacatcaaag ccccagtgct caggaaattt ctgatcttct
    58981 gcggcaatca ccgccagtcc catccacggc gagatttgat ccatactgac ccagtcagaa
    59041 tgtgccacgt aacgaaaatt gccatgcagc caggcgctga cctgtcgctc gaccattacc
    59101 gctgagaagg gaacaggcgc aacgctaaac aacgcgatgc ccccgcccca gaaaacggcg
    59161 aggacaacca ttaaccgcaa aagaaaacgg cgaacgaaac taaacaccgt taagcggctt
    59221 ttactcattc agccagaacc agcacgcggg aaaccagctt atcaatgccg ctcgccgctt
    59281 ctgcaatgtt ctgcgccagc atatatgctg gggtggtgac aatcttattg tcttcatcaa
    59341 ccacgatatc atcgacagga cacggcacat gctccgcgcc catctcttcc agcacttctg
    59401 cggtatcgat atcagtaccg atggtcaaac gcagcgggaa atcgaaaatt ttcggcagca
    59461 tcgccggggc aatacacata aaaccaagcg gttttccggc ctgatgcatc gcttgtgcca
    59521 gcgcctttaa ttcacggtca acggtgcatt cgctaccaag actggcaaaa ttgcttaaat
    59581 tcttcgccgc gccaaacccc cccggcacaa tcaacgcatc cagttcagcg gcatcggcct
    59641 gggccagagg acggatttca ccgcgcgtta ttcgtgccgc ttcaatcagc acattgcgcg
    59701 tttccgtcat cgcttcgcca gttaaatggt tgataacatc aacctgctgc ttatccggtg
    59761 caaagcagac cgcctgcgca ccgctgcgtg aaatagctaa caacgtcaac accgcttcat
    59821 gaatttcaga accgtcatag acgccgcatc cgctcagaat tacgccaatt ttcttcattg
    59881 tgatcatcct ttcgcaattg actgaaacac attaattttt ttaataaaaa tggtacgcat
    59941 cacacattta actgattcat gtaacaaatc atttaagttt tgctatctta actgcgtgcg
    60001 gcctgaaaaa cagtgctgtg cccttgtaac tcatcataat aatttacggc gcagccaaga
    60061 tttccctggt gttggcgcag tattcgcgca ccccggtcta gccggggtca ttttttagtg
    60121 gcttttgcca cccacgcttt cagcacttct acgtcgtgac gccactcttc tttcatctct
    60181 tcaatccatt caccgacgtt atcttcccag gcgggaaggt caggagactg aatttgctga
    60241 cccagctgtt gcagatggcg taaccccact gaccccgccg cacctttaat tttatgtcct
    60301 tcctcaacaa tgcctttttt atcctgcgcc gtcagattcg actccagcac gctgacatag
    60361 cccggcatca tcttctcaaa caccgctaac ccgtcggtga tcagcttcgg tcctacaagt
    60421 tcgagatact gttccagcat gggaatatcg agcaatgctt ctgatttact gttctcttct
    60481 gtcgtcaccg tactctcctc atcatcctgg gtatcccaga atttcttgat catcgcggtt
    60541 agcgccggaa ccgaaagcgg cttactcagc acatcatcca ttccagcatt gaggtactct
    60601 tgtttgtctt tcagcacgtt agcggttaag gccaccagcg gcggtaaatc ctcgcgcgga
    60661 taacgcttcg tcagttcacg agagatatcc agcccggtca tatctggcaa ctgaatatcc
    60721 agcaacacca ggtcgtattc gcccggttta aacatctcca gcgccgcctt gccggtcatg
    60781 gcgacatcaa cgctgttacc taatttttcc agcacagaac gcgcgacaat cacgttcagt
    60841 tcaatgtctt ccaccagcag cacattcagc gccggtaaag gcatatcgtc ttcatcaaac
    60901 gcatcatcga cctcttctgc caccgacggt gcgtggatcg tcaacgtaaa ggttgaacct
    60961 ttgccctgtt cactggtaac cgtaatatcg ccgcccatat ttttcgccag acgacgagaa
    61021 acggccagac caataccggt gccggtggca ggtttaccgc catgactgtc tttcacctgg
    61081 taatacatgg cgaagatttt atccagctca tcctgcggaa tgccgatacc agaatcttcc
    61141 acttcaaaat gcagcatatc gccttcatcg tagcgcacgc gcacggtaac ctggccttgc
    61201 tgggtgaatt tgacggcgtt actgatgagg ttccacagga tctgccgtaa acgcgtcccg
    61261 tcggtaatga cctgatgcgg taatggcagc gtaggctcca ggttaaagcg caatcctttt
    61321 tgttgcgcct gcaatgcgga gagattttcc agatcggcaa ggaagctggt gaaatcaacc
    61381 ggctgattat caagctggac cttgcgccgt tccatcttat ccatgtcgat aatatcgtta
    61441 aagatattcc ccagcgtgac ggccgaaaca tggatagttt tgagatattt ttcctgctcg
    61501 gcggtgagtt cggtatccag cagaatgcgg ctcaggccga cgataccgtt cagcggtgta
    61561 cgcaattcgt gactgatggt ggaaataaac gtcgttttgt cgcggctggc gcgttcaagc
    61621 gcatcctgat accgcttacg ctcggtaatg tcgcgaccaa agcccatcaa accgtgacgt
    61681 ttacccacgc ggtcgtagta cggcacttta cggatttcaa agcaggcttt gcgcccgtcc
    61741 gggtaatcca gccactgttc ataggtcagt gacacattat gacggaacac tttttcatcg
    61801 gtttcaatga cttttgcggc ggcttccggt gagtaaacat cagcaggttt caggtgaacc
    61861 agttgttttt cgctttttcc ggtcagcagc tccatcgcgc ggttacagcc ggaaaactct
    61921 ttatcttcgt tacgataaaa aaccaagtcg ggtgaagcat caaggaagga acgtaagaat
    61981 gaggattgct gctcgagctg aatttgtgtc tcttcgcgct ctttgatttc aattttcagt
    62041 tggccgaagg tttcctgcag ttctgcttcc gctttttcac gaacggcaat ttcctgattt
    62101 agctgggcaa tattatcttt taactgaacg ttgaggctca aatcgcgctc gcgcatctcc
    62161 tccagttttt gcaccagccg tgacagacgt tgtcgtgact cctccagttg ctcgacgacc
    62221 accgatagaa agtagaccgc ccacggcgta atcagcaaac caaagaagat agaacgaata
    62281 acatcaatgc tttcgacctg accatgcagc accatggtta ccgccatttg taccacaatg
    62341 gcaagaacga cgagggccag cgccagcaac attgagaagc gcaccagacc taacttcatc
    62401 atcaggtcaa cataatactg cgccagcaga cgaatttgct tcattaggga attccttcac
    62461 gacaacctgc cccaataata cccaattctg acagttacgt tataaattgt gtgagaaatg
    62521 cggaattccc ctcaacgacc tactccgttg gaggtagcca cggacgtccc agcgccgatc
    62581 cctgtacgcc atgttcattc agatagcgat ccagctcgac cattcccgtc catcgatttt
    62641 cgcaccacag cggagcaagc agcgtcgggc gacgggcgct ggcggaaatg cggtggtaga
    62701 tcacttccgg tggcgtatgg cgaatcattt ctccggcagt gagcgtataa tcctccagtt
    62761 caataccgtt caaacgcccc gcttcccagg ctttcgccat aatgctgcct ttcacaatat
    62821 gcagcggatg cagctttatg ccgtccacgc tggtttcaac cactcgctcc agcgtttgca
    62881 aacattcagc ctgcccttcg ccaggcaggc cgacaattaa gtgggaacac accttcagcc
    62941 cgcgctgacg tgccagctgg gttgtacgct gataacaggc aaaatcatgg ccgcggttga
    63001 tgcgatgcag tgttttgtcg tgggcggttt gtagccccag ctccagccac acttcgtaac
    63061 cctggtcctt atattcgcaa agcagatcca gcaccgcatc cggcacgcag tccgggcggg
    63121 taccaacaca caaaccgaca atattggcct ggctcaccgc ctgctgatac atagaacgca
    63181 gcacctgaac ttccgcaaag gtgctggtat acgcctgaaa gtaggccaga tagcgtttag
    63241 cgcggttaac taaattcgcc tggtgcgcca gttgctcggc aatggaacga tgctgctgcg
    63301 cttcatcggc aaacgaggca acattacaga atgtgcagcc accacgcccg atggtaccgt
    63361 cacggttagg gcagctaaaa ccgccgtgca gcgtcagctt atgcaccttt tgcccataac
    63421 gacgggtgag atcaccacca aacatattga ctaatttctg taactgcata atctgataga
    63481 ccgcgccttg aaaagaggcc aaagcctgcc atttttagct caattcggcg atgacctgga
    63541 tcaatcgtcc cggcctgctt ttatcaactg cataatcaat caaaattacc gaaatttcat
    63601 gcacaatcac ataaatcact tttgcttatc ttgtgtcaga tttttttatc tccttatgga
    63661 ttttaggcaa aaacagtagc atgaaacgtc attaccaatt aaggcagtat aaaatgctgg
    63721 ttttctcgtc agttcaaggc aggataaggg ttaacacgcc tttatgacag tcaggaattg
    63781 actgtttctc taacgactct ccttttagcc ttaaagataa aatctatttt aatttcagct
    63841 atttaataaa gaattttgcg ctaaagcaca tttctgtacc aataagcttg ccatttgacc
    63901 tgtatcagct ttcccgataa gttggaaatc cgctggaagc tttctggatg agcagcctgc
    63961 tcatcatatt tatgcagtaa ttgagatccc ctcttcaccg tattaaccga tgcgaaaagg
    64021 acaacaaggg ggcgaatgcg aggcgcgcgt atgacacgca aaccccgtcg ccacgctctt
    64081 tctgtgcccg tgcgcagcgg ttcggaagtg gggttcccgc agagcctggg ggaggttcac
    64141 gatatgttgt acgataaatc ccttgagagg gataactgtg gtttcggcct gatcgcccac
    64201 atagaaggcg aacctagcca caaggtagtg cgtactgcaa tacacgcact ggcccgcatg
    64261 cagcaccgtg gcgcgattct cgccgatggt aaaaccggcg acggttgcgg cttgctgtta
    64321 caaaaaccgg atcgcttttt tcgcatcgtt gcgcaggagc gcggctggcg tttagcaaaa
    64381 aactacgctg tcgggatgct cttcctgaat aaagatcctg aactcgccgc tgccgcacgc
    64441 cgcatcgttg aagaagaact gcaacgcgaa accttgtcga ttgtgggctg gcgtgatgtc
    64501 cccactaacg aaggcgtgct gggtgaaatc gccctctcct ctctgccacg cattgagcaa
    64561 atttttgtga acgccccggc aggctggcgt ccacgcgata tggagcgtcg tctgtttatc
    64621 gcccgccgcc gcattgaaaa gcgtctcgaa gccgacaaag acttctacgt ctgtagcctg
    64681 tcgaatctgg tgaacatcta taaaggtctg tgtatgccgg cggatctgcc gcgcttttat
    64741 ctggatcttg cggacctgcg tctggaatcg gccatttgcc tgttccacca gcgcttctcc
    64801 actaacaccg taccgcgctg gccgctggcg caaccgttcc gctatctggc gcataacggt
    64861 gaaatcaaca ccatcaccgg taaccgccaa tgggcgcgtg cgcgtaccta taaattccag
    64921 acgccgctga tccctgacct gcacgacgcc gcgccattcg tcaacgaaac cggttctgac
    64981 tccagttcca tggataacat gctggaactg ctgctggcag gcgggatgga tatcatccgc
    65041 gccatgcgcc tgttagtgcc acccgcctgg cagaacaacc cggatatgga cccggaactg
    65101 cgtgccttct ttgactttaa ctccatgcat atggagccgt gggatggtcc ggcgggcatc
    65161 gtgatgtccg atggtcgctt cgctgcctgt aacctcgacc gtaacggtct gcgtccggca
    65221 cgctacgtca ttaccaaaga caagctcatc acctgcgcct ctgaagtcgg tatctgggat
    65281 taccagcctg acgaagtggt cgaaaaaggc cgcgtcgggc caggcgaact gatggtgatc
    65341 gacactcgca gtgggcgtat tctgcactcg gcagaaaccg atgacgatct gaaaagccgc
    65401 cacccttata aagagtggat ggagaaaaac gtccgccgac tggtaccgtt tgaagatctg
    65461 cccgatgaag aagtgggtag ccgcgaactg gacgacgaca cgcttgccag ctaccagaaa
    65521 cagtttaact acagcgcgga agagctggac tccgtaattc gcgtactggg cgaaaacggt
    65581 caggaagcgg tcggttcgat gggcgatgat accccattcg ccgtgctctc cagtcagccg
    65641 cgcattattt acgactactt ccgccagcag tttgcccagg tgactaaccc gccaatcgac
    65701 ccgctgcgtg aagcgcatgt tatgtcgctc gccaccagta tcggtcgtga aatgaacgtc
    65761 ttttgcgaag cagagggcca ggcgcaccgt ttaagcttta aatcgccgat tctgctctac
    65821 tccgatttca aacagctcac gacgatgaaa gaggagcact accgcgcaga tacgctggat
    65881 atcacctttg acgtcactaa aaccacgctc gaagcgacag tcaaagagct atgcgacaaa
    65941 gccgaaaaaa tggtacgtag cggcaccgtg ttgctggtgc tctccgaccg gaatatcgct
    66001 aaagatcgcc tgccggttcc ggctccgatg gcggttggcg cgatccagac ccgtctggtc
    66061 gatcaaagcc tgcgttgcga tgccaacatc atcgtcgaaa ccgccagcgc ccgcgatccg
    66121 caccacttcg ccgtgttgct gggtttcggc gcgacggcta tttatccgta ccttgcctat
    66181 gaaacgctgg gccgcctggt agacacccac gcgattgcca aagattatcg taccgtgatg
    66241 ctcaactacc gtaacggcat caacaaaggc ttgtacaaaa tcatgtccaa aatgggcatc
    66301 tccaccatcg cctcttaccg ctgctcgaaa ctgtttgaag cggtcggtct acacgatgat
    66361 gtagtgggcc tgtgcttcca gggcgcggtc agccgcattg gtggggcaag ctttgaagac
    66421 ttccagcagg atctgctgaa tctgtcgaaa cgtgcctggc tggcgcgcaa gccgattagc
    66481 cagggcggcc tgctgaaata cgtccacggc ggcgaatacc acgcctacaa cccggacgtg
    66541 gtgcgcacgc tgcaacaggc ggtacaaagc ggcgagtaca gcgactatca ggaatacgcg
    66601 aagctggtta atgagcgtcc ggcaaccacg ctgcgcgatc tgctggcaat tacgccgggt
    66661 gaaaacgcgg tcaacattgc tgatgttgaa ccggcaagcg aactgtttaa acgctttgat
    66721 accgccgcga tgtctatcgg cgcgttaagc ccggaagccc acgaggcgct ggcggaagcg
    66781 atgaatagca tcggcggtaa ctcgaactcc ggtgaaggcg gcgaagaccc ggcgcgctat
    66841 ggcactaaca aagtgtcgcg catcaagcag gtggcttctg gtcgcttcgg cgttactccg
    66901 gcgtatctgg tcaatgccga cgtcattcag attaaagtcg cccagggcgc gaagccgggc
    66961 gaaggtggtc agttgccggg cgacaaagtc actccttaca tcgccaaact gcgctattcg
    67021 gtgcccggag tgacgctgat ctccccgccg ccgcaccacg atatctactc tatcgaagac
    67081 ttagcgcagc ttattttcga cctcaagcag gttaacccga aagcgatgat ctccgttaag
    67141 ctggtgtccg aaccaggcgt tggcaccatc gcgactggcg tggcgaaagc ttatgcggac
    67201 ttgatcacca tcgcgggcta tgacggcggc accggcgcaa gcccgctgtc gtcagtgaaa
    67261 tatgcaggct gcccgtggga actcggtctg gtggaaaccc aacaggcgct ggttgccaac
    67321 ggcttgcgtc ataaaattcg tttgcaggtt gatggcggcc tgaaaacggg tgtcgatatc
    67381 atcaaagcgg cgattctcgg cgcagaaagc ttcggcttcg gcactggccc aatggtggcg
    67441 ctcggctgta aatatctgcg tatttgccac ctgaacaact gcgccacggg tgtagcaact
    67501 caggatgaca aactgcgtaa gaatcactat cacggcctgc cgttcaaggt gactaattac
    67561 tttgagttta tcgcccgtga aacccgcgag ctgatggcac agctgggagt aacgcgtctg
    67621 gtggatctga ttggtcgcac cgacctgctg aaagagctgg acggtttcac cgccaaacag
    67681 cagaaactgg cgctgtcgaa gctgctggag actgccgagc cacatgcagg taagtcactc
    67741 tactgcactg aaaacaaccc gccgtttgat aacggcctac tgaacgcgca gttgctgcaa
    67801 caggcgaaac cgtttgtcga tgaacgccag agcaaaacct tctggttcga tatccgcaac
    67861 accgaccgtt ctgtcggcgc gtcgctttca ggctatatcg cccagacgca cggcgatcag
    67921 gggctggcag ccgatcctat caaagcgtac ttcaacggca ccgcaggcca gagtttcggt
    67981 gtgtggaacg cgggcggcgt ggaactgtac ctgaccgggg atgccaacga ctatgtcggt
    68041 aaaggcatgg cgggcggctt aatcgccatt cgtcctccgg ttggttccgc cttccgcagc
    68101 catgaagcaa gcattatcgg caacacctgc ctgtatggcg cgaccggtgg tcgtctgtat
    68161 gccgcaggcc gcgcgggtga acgtttcggc gtgcggaact ccggtgctat caccgtggta
    68221 gaaggcattg gcgacaacgg ctgtgaatat atgacgggcg gtattgtctg tattctgggt
    68281 aaaaccggcg ttaacttcgg cgcgggcatg accggcggct tcgcttacgt tctcgatgaa
    68341 agcggtgatt tccgcaaacg cgttaacccg gaactggtcg aagtcttaag cgtcgacgat
    68401 ctggcgatcc atgaagagca tctgcgcggt cttatcaccg agcatgttca gcataccggt
    68461 tctcagcgcg gtgaagagat tctggcgaac tggtcaacct tcgccacgaa attcgcgctg
    68521 gttaaaccga agtccagtga tgtaaaagca ctgctgggtc accgtagtcg tagcgcagca
    68581 gagttgcgcg tgcaggcgca gtaaggggta gcaacaatga gtcagaatgt ttatcaattt
    68641 atcgacctgc agcgcgttga tccgccaaag aaaccgctga agatccgtaa aattgagttt
    68701 gttgaaattt acgagccgtt ttccgaaggc caggccaaag cgcaggctga ccgctgtctg
    68761 tcgtgcggca acccatactg cgagtggaaa tgcccggtac acaactacat cccaaactgg
    68821 ctgaagctcg ccaacgaggg gcgtattttt gaagcggcag aactgtcgca tcagaccaac
    68881 acactgccgg aagtatgcgg acgtgtctgc ccgcaagacc gtctgtgcga aggttcctgc
    68941 accctgaacg atgagttcgg tgcggtgacc atcggcaaca ttgagcgtta tatcaatgat
    69001 aaagcgttcg aaatgggctg gcgtccggat atgtccggcg tgaagcaaac cggtaaaaaa
    69061 gtggcgatta tcggtgcagg cccggcaggt ctggcgtgtg cggatgtcct gacgcgtaac
    69121 ggcgtaaaag ccgttgtctt cgaccgtcat ccagaaattg gcgggctgct gaccttcggt
    69181 attccggcct tcaagctgga aaaagaggta atgacgcgtc gccgtgaaat cttcaccggc
    69241 atgggtattg aattcaaact caataccgaa gtgggccgcg acgtacagct ggacgatctg
    69301 ctgagtgatt acgatgccgt gttccttggc gttgggactt atcagtcaat gcgcggcggg
    69361 ctggaaaacg aagacgccga tggcgtgtac gcagcgctgc cgttccttat cgccaacacc
    69421 aaacagttaa tgggctttgg tgaaacccgc gacgaaccgt tcgtcagcat ggaaggcaaa
    69481 cgcgtggtag tacttggcgg tggcgacact gcgatggact gcgtgcgcac gtctgtacgc
    69541 cagggggcaa agcacgttac ctgtgcctat cgtcgtgatg aagagaacat gccgggttcc
    69601 cgccgcgaag tgaaaaacgc gcgggaagaa ggcgtagagt tcaaattcaa cgtccagcca
    69661 ctggggattg aagtgaacgg taacggcaaa gtcagcggcg taaaaatggt acgtaccgaa
    69721 atgggcgaac cggatgccaa aggccgtcgc cgcgcggaga tcgttgcagg ttccgaacat
    69781 atcgttccgg cagatgcggt gatcatggcg tttggtttcc gtccacacaa catggaatgg
    69841 ctggcaaaac acagcgtcga gctggattca caaggccgca tcatcgctcc ggaaggcaac
    69901 gacaacgctt tccagaccag caacccgaaa atctttgctg gcggcgatat cgtccgtggt
    69961 tccgatctgg tcgttaccgc catcgccgaa ggtcgcaaag cggcagacgg cattatgaac
    70021 tggctggaag tttaagctct gtcccagcgc cgcctcctca gaaaagagtc ggcgctgaaa
    70081 ttctcctttc tttctggaaa tcgcctcgca taatctgaag aaaaccaatg taatacgctc
    70141 atctggattg ttaccggtag atgcccggcg tatagctgat tccattattc aacgcagcgc
    70201 catataaagg gacagataat ggaatcgctc tcagagagaa cctcaacagg ctaccagcaa
    70261 atccacaacg gcattattca tctggtcgat agcgcccgaa cggaaacggt acgtagcgtt
    70321 aacgcgctga tgaccgcgac gtactgggaa attggccgac ggattgtcga atttgaacaa
    70381 ggtggtgagg ccagagcagc ttatggtgca cagttgattg atcggctatc gaaggattta
    70441 agtcgacggt ataaacgtgg ggtctctgca aaaaacttac gccaaatgag gcttttttac
    70501 ctcttttttc aacatgttga aattcgccag acagtgtctg gcgaattaac accattggga
    70561 attccgcaga caccatctgc agaattccca tcggcaaaga tttggcagac gctgtctgcc
    70621 aaattgagag gctgtttgat atttccacag cttttgtatt gcatctccta ttttcggttt
    70681 gtatattcat gcaaataaac gaagga
//
LOCUS       DAJTRD010000007        67449 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTRD010000007 DAJTRD010000000
VERSION     DAJTRD010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67449
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.007"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..229
                     /locus_tag="OO315_000529"
     CDS             <1..229
                     /locus_tag="OO315_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259191.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
                     /protein_id="HCR5889561.1"
                     /translation="ESNSDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLD
                     KYGDARINASYTQLEIKHSQPKNRASGKRVS"
     gene            624..1263
                     /locus_tag="OO315_000530"
                     /pseudo
     CDS             624..1263
                     /locus_tag="OO315_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            1366..1689
                     /locus_tag="OO315_000531"
     CDS             1366..1689
                     /locus_tag="OO315_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5889562.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO315_000532"
     CDS             complement(1756..3213)
                     /gene="nuoN"
                     /locus_tag="OO315_000532"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5889563.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO315_000533"
     CDS             complement(3220..4749)
                     /gene="nuoM"
                     /locus_tag="OO315_000533"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5889564.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO315_000534"
     CDS             complement(4913..6754)
                     /gene="nuoL"
                     /locus_tag="OO315_000534"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5889565.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO315_000535"
     CDS             complement(6751..7053)
                     /gene="nuoK"
                     /locus_tag="OO315_000535"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5889566.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO315_000536"
     CDS             complement(7050..7604)
                     /gene="nuoJ"
                     /locus_tag="OO315_000536"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5889567.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO315_000537"
     CDS             complement(7616..8158)
                     /gene="nuoI"
                     /locus_tag="OO315_000537"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5889568.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO315_000538"
     CDS             complement(8173..9150)
                     /gene="nuoH"
                     /locus_tag="OO315_000538"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5889569.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO315_000539"
     CDS             complement(9147..11873)
                     /gene="nuoG"
                     /locus_tag="OO315_000539"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5889570.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO315_000540"
     CDS             complement(11926..13263)
                     /gene="nuoF"
                     /locus_tag="OO315_000540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5889571.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO315_000541"
     CDS             complement(13260..13760)
                     /gene="nuoE"
                     /locus_tag="OO315_000541"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5889572.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO315_000542"
     CDS             complement(13763..15565)
                     /gene="nuoC"
                     /locus_tag="OO315_000542"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5889573.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO315_000543"
     CDS             complement(15659..16321)
                     /gene="nuoB"
                     /locus_tag="OO315_000543"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5889574.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO315_000544"
     CDS             complement(16337..16780)
                     /gene="nuoA"
                     /locus_tag="OO315_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5889575.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO315_000545"
     CDS             complement(17411..18349)
                     /gene="lrhA"
                     /locus_tag="OO315_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5889576.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            19268..20485
                     /gene="alaA"
                     /locus_tag="OO315_000546"
     CDS             19268..20485
                     /gene="alaA"
                     /locus_tag="OO315_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5889577.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20569..21168
                     /gene="yfbR"
                     /locus_tag="OO315_000547"
     CDS             20569..21168
                     /gene="yfbR"
                     /locus_tag="OO315_000547"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5889578.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(21227..23059)
                     /locus_tag="OO315_000548"
     CDS             complement(21227..23059)
                     /locus_tag="OO315_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5889579.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILTVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(23146..23796)
                     /gene="hxpA"
                     /locus_tag="OO315_000549"
     CDS             complement(23146..23796)
                     /gene="hxpA"
                     /locus_tag="OO315_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5889580.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23807..24301)
                     /locus_tag="OO315_000550"
     CDS             complement(23807..24301)
                     /locus_tag="OO315_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5889581.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24384..24839)
                     /locus_tag="OO315_000551"
     CDS             complement(24384..24839)
                     /locus_tag="OO315_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5889582.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            25177..26379
                     /gene="ackA"
                     /locus_tag="OO315_000552"
     CDS             25177..26379
                     /gene="ackA"
                     /locus_tag="OO315_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5889583.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26454..28598
                     /gene="pta"
                     /locus_tag="OO315_000553"
     CDS             26454..28598
                     /gene="pta"
                     /locus_tag="OO315_000553"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5889584.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28788..30308
                     /gene="yfcC"
                     /locus_tag="OO315_000554"
     CDS             28788..30308
                     /gene="yfcC"
                     /locus_tag="OO315_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5889585.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(30341..30883)
                     /gene="yfcD"
                     /locus_tag="OO315_000555"
     CDS             complement(30341..30883)
                     /gene="yfcD"
                     /locus_tag="OO315_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5889586.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30941..31492)
                     /gene="yfcE"
                     /locus_tag="OO315_000556"
     CDS             complement(30941..31492)
                     /gene="yfcE"
                     /locus_tag="OO315_000556"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5889587.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31548..32192)
                     /gene="yfcF"
                     /locus_tag="OO315_000557"
     CDS             complement(31548..32192)
                     /gene="yfcF"
                     /locus_tag="OO315_000557"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5889588.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            32328..32975
                     /gene="yfcG"
                     /locus_tag="OO315_000558"
     CDS             32328..32975
                     /gene="yfcG"
                     /locus_tag="OO315_000558"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5889589.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            33032..33394
                     /gene="folX"
                     /locus_tag="OO315_000559"
     CDS             33032..33394
                     /gene="folX"
                     /locus_tag="OO315_000559"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5889590.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33415..34308
                     /locus_tag="OO315_000560"
     CDS             33415..34308
                     /locus_tag="OO315_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5889591.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(34356..35246)
                     /gene="rpnB"
                     /locus_tag="OO315_000561"
     CDS             complement(34356..35246)
                     /gene="rpnB"
                     /locus_tag="OO315_000561"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5889592.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35443..36216)
                     /gene="hisP"
                     /locus_tag="OO315_000562"
     CDS             complement(35443..36216)
                     /gene="hisP"
                     /locus_tag="OO315_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5889593.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(36224..36940)
                     /locus_tag="OO315_000563"
     CDS             complement(36224..36940)
                     /locus_tag="OO315_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5889594.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36937..37623)
                     /gene="hisQ"
                     /locus_tag="OO315_000564"
     CDS             complement(36937..37623)
                     /gene="hisQ"
                     /locus_tag="OO315_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5889595.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37713..38495)
                     /gene="hisJ"
                     /locus_tag="OO315_000565"
     CDS             complement(37713..38495)
                     /gene="hisJ"
                     /locus_tag="OO315_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5889596.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38716..39498)
                     /gene="argT"
                     /locus_tag="OO315_000566"
     CDS             complement(38716..39498)
                     /gene="argT"
                     /locus_tag="OO315_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5889597.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39764..40333)
                     /gene="ubiX"
                     /locus_tag="OO315_000567"
     CDS             complement(39764..40333)
                     /gene="ubiX"
                     /locus_tag="OO315_000567"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5889598.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40428..41945)
                     /gene="purF"
                     /locus_tag="OO315_000568"
     CDS             complement(40428..41945)
                     /gene="purF"
                     /locus_tag="OO315_000568"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5889599.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41982..42470)
                     /gene="cvpA"
                     /locus_tag="OO315_000569"
     CDS             complement(41982..42470)
                     /gene="cvpA"
                     /locus_tag="OO315_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5889600.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42729..43391)
                     /gene="dedD"
                     /locus_tag="OO315_000570"
     CDS             complement(42729..43391)
                     /gene="dedD"
                     /locus_tag="OO315_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5889601.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43381..44649)
                     /gene="folC"
                     /locus_tag="OO315_000571"
     CDS             complement(43381..44649)
                     /gene="folC"
                     /locus_tag="OO315_000571"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5889602.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44719..45633)
                     /gene="accD"
                     /locus_tag="OO315_000572"
     CDS             complement(44719..45633)
                     /gene="accD"
                     /locus_tag="OO315_000572"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5889603.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45789..46448)
                     /locus_tag="OO315_000573"
     CDS             complement(45789..46448)
                     /locus_tag="OO315_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5889604.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46531..47343)
                     /gene="truA"
                     /locus_tag="OO315_000574"
     CDS             complement(46531..47343)
                     /gene="truA"
                     /locus_tag="OO315_000574"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5889605.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(47343..48356)
                     /locus_tag="OO315_000575"
     CDS             complement(47343..48356)
                     /locus_tag="OO315_000575"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5889606.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48422..49558)
                     /gene="pdxB"
                     /locus_tag="OO315_000576"
     CDS             complement(48422..49558)
                     /gene="pdxB"
                     /locus_tag="OO315_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5889607.1"
                     /translation="MKILVDENMPYARDLFSRLGEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49657..50652
                     /gene="flk"
                     /locus_tag="OO315_000577"
     CDS             49657..50652
                     /gene="flk"
                     /locus_tag="OO315_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5889608.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50649..51827)
                     /locus_tag="OO315_000578"
     CDS             complement(50649..51827)
                     /locus_tag="OO315_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5889609.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(52082..53323)
                     /gene="fabB"
                     /locus_tag="OO315_000579"
     CDS             complement(52082..53323)
                     /gene="fabB"
                     /locus_tag="OO315_000579"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5889610.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53482..55488
                     /gene="mnmC"
                     /locus_tag="OO315_000580"
     CDS             53482..55488
                     /gene="mnmC"
                     /locus_tag="OO315_000580"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5889611.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55609..55887)
                     /locus_tag="OO315_000581"
     CDS             complement(55609..55887)
                     /locus_tag="OO315_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5889612.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55921..56469)
                     /gene="epmC"
                     /locus_tag="OO315_000582"
     CDS             complement(55921..56469)
                     /gene="epmC"
                     /locus_tag="OO315_000582"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5889613.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56469..57278)
                     /locus_tag="OO315_000583"
     CDS             complement(56469..57278)
                     /locus_tag="OO315_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5889614.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(57278..58102)
                     /gene="mepA"
                     /locus_tag="OO315_000584"
     CDS             complement(57278..58102)
                     /gene="mepA"
                     /locus_tag="OO315_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5889615.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(58106..59191)
                     /gene="aroC"
                     /locus_tag="OO315_000585"
     CDS             complement(58106..59191)
                     /gene="aroC"
                     /locus_tag="OO315_000585"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5889616.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(59226..60158)
                     /gene="prmB"
                     /locus_tag="OO315_000586"
     CDS             complement(59226..60158)
                     /gene="prmB"
                     /locus_tag="OO315_000586"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5889617.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            60324..60875
                     /gene="smrB"
                     /locus_tag="OO315_000587"
     CDS             60324..60875
                     /gene="smrB"
                     /locus_tag="OO315_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5889618.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60979..61869)
                     /locus_tag="OO315_000588"
     CDS             complement(60979..61869)
                     /locus_tag="OO315_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5889619.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61856..62380)
                     /locus_tag="OO315_000589"
     CDS             complement(61856..62380)
                     /locus_tag="OO315_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5889620.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62377..62847)
                     /locus_tag="OO315_000590"
     CDS             complement(62377..62847)
                     /locus_tag="OO315_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5889621.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62844..63392)
                     /locus_tag="OO315_000591"
     CDS             complement(62844..63392)
                     /locus_tag="OO315_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5889622.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63397..64119)
                     /locus_tag="OO315_000592"
                     /pseudo
     CDS             complement(63397..64119)
                     /locus_tag="OO315_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(64139..64861)
                     /locus_tag="OO315_000593"
     CDS             complement(64139..64861)
                     /locus_tag="OO315_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5889623.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64921..66075)
                     /gene="yfcU"
                     /locus_tag="OO315_000594"
                     /pseudo
     CDS             complement(64921..66075)
                     /gene="yfcU"
                     /locus_tag="OO315_000594"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(66024..66779)
                     /locus_tag="OO315_000595"
     CDS             complement(66024..66779)
                     /locus_tag="OO315_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5889624.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66861..67424)
                     /locus_tag="OO315_000596"
     CDS             complement(66861..67424)
                     /locus_tag="OO315_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5889625.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16840 a        18248 c        16745 g        15616 t
ORIGIN      
        1 ggaaagcaat tctgacagcg ccccctatac gttattaaaa aactatgctg acagatttaa
       61 aaaatattca gcagaagagc agtacgaaat tgattttcaa catcgactgg taaacagaaa
      121 ttgttattta gataaatatg gcgatgcacg aatcaatgct tcttatacac aattagaaat
      181 aaaacactca caacccaaaa atagagcatc cggcaaaaga gtgagttaac atcattgtta
      241 atcaattcga ccagtcagta aatatgctga ctggtcgaac ataatttgct aaaaagtcat
      301 cactgactga tgtcagtccc accatctgcc agttcaatct acgggtattt ccagcatatc
      361 gcggcacgcg attgctggtc tgttgatgta gtggattgta gttgctgact tttcttaccc
      421 tcagatttct gccccacttg tcaattcaat ctgccaataa ataacataat tatattttta
      481 ttcctcacag aaattccttt ttaaaaatag cgaacccact gtaaaaaaca ctttttgtat
      541 acctctacca agcgtaataa aataaacgct cataattcat tataaataat aatgaaataa
      601 cgccttttat tgataattta catatgaaaa aattttcctt tttttatatt atttctgttt
      661 tcactccctc tgattatttt taccaaccct gggtcactgc attatcgcca acgccccgac
      721 acgtaagtcc tgagcaatta gaaaaaacag ttcgttatct tacacaaact gtgcatccac
      781 gtaatgccgg caatattgat aatctgaata ggtccgcaga gtatataaaa gagttctttg
      841 tcagtagcgg tgccagagtt accgcgcagg gcgtccccga ttggctatta cgactctgcg
      901 cctggaagcc agaattaccc ttacccggca atgtcctggc tttatcccga tcggggagac
      961 tttattgctg tggtcggcag aatgcaggat atcaacgccg ttcgtcaggt aaaagcggca
     1021 ttgttgtcat cccgggattt atctgtttat tctatgaatg ccccagggtt tattcccggt
     1081 attgattttt ctgatcacct caattattgg caacacgata ttcccgccgt aatgattact
     1141 gacaccgctt tttatcgtaa taaacaatac cacttgcccg gtgataccgc agacagattg
     1201 aattatcaga aaatggctca ggtggtagat ggtgttataa ccttgttata caacagtaaa
     1261 taataaaaat ttctcgaggc gggtccagaa acagcgacat gtacatttta ttttccctcg
     1321 tctgttttaa gatgcgaaac ataaaattca atggaggaaa aataaatggg aatgattggc
     1381 tattttgcag aaattgactc agaaaagatt aaccaactgc ttgagtctac tgaaaaacct
     1441 ttgatggata atatacatga cacgctttca ggattacgcc gactggaatc actggatcga
     1501 agtgagctgc gtaaacagtt ttccattaag cgactcaatg aaatggagat ttatccggga
     1561 gtgacctttt cagaagagtt agaggggcaa ctctttgcct cgatcatgct tgatatggaa
     1621 aagctgatta gcgcgtatcg acgtatgctg cgacaaggga atcatgcgct gacggttatt
     1681 gttgggtgat ggtgtcagag cgccaaaaaa aaccgccgaa catgtcggcg gctttctgac
     1741 ttacaaacta acagtttaca tcagcggcat tgctaaccgc acaatactaa tcagcggttg
     1801 tggccataca cccagcacca gtaccaacag tgcagaaatc agcaccacga taccgcccgc
     1861 gctgtactgc cagtttgacg gtgcatcgcg acccggttgt tccggtgcgt gcaggtacag
     1921 gctcaccgcc acgcgcaggt agtagtagag gccgattgcc gaaccgacaa ccacggcacc
     1981 caccagccac cacaagtgtg cctggacacc gaccgccagc acgtagaact taccgataaa
     2041 gcccagcgtc atcgggatac cggccagaga cagcatcatc accgtcatca ctgccgcgag
     2101 gatcggacga tgccagaaca gaccgcggta agagaacagg gaatcagcat ccgggccacg
     2161 atacgggctg gacatcaggc tgaccacgcc gaacgcgccg aggctgctga acagataacc
     2221 ggccaggtaa acccctaccg cttccatcga catctcgccg gtttgcagcg caatcagcgc
     2281 caccagcaga tagccgaggt gagagataga tgagtaaccg agcagacgtt tgatgttggt
     2341 ctggctcaac gccatcaggt taccgaagat gatggaggca aaggcgataa tcgccagtac
     2401 tacgcgaatc gcttcgctgt cacccaccgg tgcgtagagg aacagacgca tcaccacacc
     2461 gaagatagcg attttgctcg ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg
     2521 gtatacgtct ggcgtccaca ggtggaacgg caccagagag agtttgaagc cgaggccaac
     2581 aatcatcatg ccgaaacctg ccagcaacag cggctcgttg agcataccgt cgccgaggtt
     2641 tttacccagc gcgacaaacg acaggtcgcc agactgcgca tacaccagcg ccataccgaa
     2701 cagcaggaaa gaagacgctg cggcagaaag gatggtgtat ttgatactgg cttccagcga
     2761 acgtttctgg cggaaagcgt aaccgaccag gccaaacagc ggcaaagaga tcagttcaat
     2821 accgaggaac agagacgcca gatggtttgc attcgccagc aggatcccgc ccagcgcggc
     2881 aattaacacc aacaggtaga actcatcctt gttgtcgtta tagccttcaa gccacgggta
     2941 ggcgaaagta caggtggcga ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa
     3001 accatcaacg cgcatcagcg gcgtaacgtc catagcgccc gcctggccaa caaaccagag
     3061 cgaaaccagc gccgcgttaa gcccaataac cgagagcgta gcgttgagga aatgattgcg
     3121 tcgccacgca atggagagca tcacaaccac caccgtcaag ccgacgatca gcaacggtag
     3181 cagtgcgatc aggttttgtg gagttattgt catggcgatt tacggccttg tagtagtaac
     3241 ggaattaaca aaccactgct ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg
     3301 ctgcggatag aagcccagca gtaccagcag caccaccagc aacaggatca taaacagctc
     3361 acgcagcgac atccctggca gttcctggct ggcaatctgg cttttcgctt taccgaagta
     3421 agcgcgatgc aacatcgcca gcgaataaac agatgcaaag accagcccaa aggtagagat
     3481 aacggtaatc accgggacaa cctggaagct gccgaacaga atcataaatt cgccgacgaa
     3541 gttaccggtg ccaggcatcc caagcgttgc caccgcaaag aacagcgaca gtgctggcag
     3601 ccatttcatc ttgctccaca gaccgcccat catgcgcatg tcgcgggtat ggatacgttc
     3661 ataaagctga ccgcagagaa taaacagacc cgccgccgac aagccgtgcg caatcatctg
     3721 gattaccgcg ccctggtagg ccaactggct gccggtgtag attgcaatca gcacgaagcc
     3781 catgtgggaa accgaggtgt aggcgatcag acgtttgata tcggtctgag cgaaggtcat
     3841 ccacgcaccg tagaagatgc cgataacacc cagccacata gcgattggcg cgaactctgc
     3901 cgacgcgttc gggaacagcg gcagggagaa acgcaacaaa ccgtaagcgg cggttttcag
     3961 caagatcccc gcgaggtcca cggaaccggc ggtcggagcc tgggagtgcg catccggcag
     4021 ccagccatgc agcggaacca ccggcatttt gactgcgaag gcgatgaaga agcccagcat
     4081 caacagatat tccacaccac tggacattgg cgtattcagc agctcttcat agttgaaggt
     4141 ccagacgccg gtcgcattgt agtgaacaaa aaccagcgcc aggatggcga tcaacatcac
     4201 cagaccactc gcctgggtgt aaatgaagaa cttggttgcc gccgtgatac gcgttttacc
     4261 gtcagaggct ttatgccccc acagtgcgat caggaagtac atcggcacca gcatcatttc
     4321 ccagaagaag aagaacagga acatgtcgat ggcaaggaac acgccgataa cgccgcccag
     4381 gatccacatc aggttgaggt ggaagaagcc ctgatatttt tcgatctctt tccacgaaca
     4441 tagtaccgcc agcacaccga gcagaccggt cagcacgacc atcagcagcg acagaccgtc
     4501 aatggcgaga tgaatagaga taccaaaacg cgggatccac ggcatgtcga attcagactg
     4561 ccactgcgga attccggcgg attgcgtcag tgaataaccg ccctgcaacc acagttgcag
     4621 cgacagcgcc agcgtcaatc ccatggtgat cagcgcgatc cagcgtggca ccttgacgcc
     4681 aaagcgttcg gtctgccagc acaggaagcc gccaataaag ggaattaata ttagccaggg
     4741 tagtaacatg gcgatcttta ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa
     4801 ttctcacgac agaaaattct cttcggactg ggggtagtgt cggatgcgcc gcgaaggcag
     4861 cttatccgac ctacgttctt atatgcccag gggcaatccc acaatcctta actcaacgca
     4921 gtaccatcaa cagtgccagc accacgaccg caccgatgct catggatgcc acataccagc
     4981 gcagataacc gttctcgctt aacagcagac ctttacctgc aaagcgggaa agaaccgccg
     5041 ggatgttcat cattgagttc agcggatcgc gtttcagcaa ccaggcaata cccaggaacg
     5101 gcttgacgaa cactttgtcg tacagccagt caaagcccca agcgttgtac caccaggtac
     5161 tcagcaggcg gcccggcgca ctgttggcga tggaggtcac cagagaacgt ttacccagcc
     5221 acagccaggc tgccagcaga atgccgacca ccgcgaccac gccagaggta atttccaggg
     5281 tcaacatgct gccgtgcgcc agttccgtcg tttgcggaag cacgccctgc agcggcggta
     5341 caatcagtgc gccaacgaag gtggaaagga tcagcagcac aatcagcggc aggctgtgag
     5401 ttaccccttt cacggcgtga gcgtgaattt gttcttttcc gtggaagacg atgaaaatca
     5461 tacggaaggt gtagagcgag gtcataaacg caccgaccag acctgccacc atcagattga
     5521 tatgaccatt cgccatcgca cccgcgagga tctcatcctt actgaagaag cccgcagtga
     5581 ccagcggcag tgccgacagt gctgcgccgc ccaccaggaa gcagagataa accagcggaa
     5641 tagatttacg cagaccgccc atcttgaaga tgttctgttc gtgatggcag gccagaatga
     5701 cggaaccgga tgccaggaac agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa
     5761 tcgccgcatc ccatgcctgc acgccaagcg cgaggaacat gtagccaatc tggctcatgg
     5821 tagagtaagc gagaacacgt ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca
     5881 gcagcgtaac cgccccgaca atacccacca gatgcagaac ttccggcgtc atcaggaaca
     5941 ggccgtgggt acgggcgatc aggtagacac ccgcggttac catcgttgcg gcgtggatca
     6001 gcgcggagac aggcgtcggg cccgccatcg cgtcagcaag ccatgtctgc aacggcaact
     6061 gcgcagattt accgaccgca ccgcccagca gcatcagcgt cgcccacatc agcatgttat
     6121 tgccgtcagc aaagtgcgct ggtgccagtt ccaccatatc gcggaagttc agggtgccca
     6181 gttcgttgta aagaatgaac agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga
     6241 cgaacgcttt cattgctgct gcgccattct tcggatcggt gtaatagaaa ccgatcagca
     6301 gataggagca caggcccacg ccttcccagc cgaggtacat cagcagcagg ttatcggcga
     6361 gaaccagaac taccatgctg gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc
     6421 cctcttcacc gcgcatgtac caggaggcgt acatgtgaat aaggaaaccc acaccagtga
     6481 ccaccgagag catggtcagc gacaggccgt ccagcaccag gttaaaaccg atgttaaagt
     6541 cgcctaccga catccacgtc cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga
     6601 aatcaacgcc gataaaggcg gttaccagcg ccgccaggcc cacagagcca acgccgacga
     6661 tcgccgaaac gttttcagac cagcgcccac gggagaacgc cagcaggacg aagccaatca
     6721 atggcaaaat aatggttaag gcaagcatgt tcatccgcgc atctcactta ctgaatcgat
     6781 gttcaggttc tggcgacgac ggtgaagttg cagcagcagc gcaaggccga tactcgcttc
     6841 tgccgccgcg aggctgatgg cgagaatgta catcacctga ccgtcggtct gaccccagta
     6901 gcttccggcg accacaaagg ccagcgcgga ggcgttaatc atgatttcca gaccaatcag
     6961 cataaacagc agattgcgac ggataaccag accggttaag ccaagaacga ataagattgc
     7021 cgcgaggatc agtccatgtt gtaaggggat catgcgtgct cctccgtttt tcttttcgcg
     7081 ctgtcgtctt tacgattgct cagcacttca cccgcacgct cttcacgacc gacgtggaag
     7141 gccacaacca gacccgcgag cagcagcata gaagccagtt ccaccgccag tacgtaaggc
     7201 ccgaacagcg taatacccac tgctttagca ctgattggcg taccgtcgat accctgatcg
     7261 ttaacaccga ggatggcgta aacaatcacc accagcatga tggccgacaa aattgccggg
     7321 ccaatccaca cctgcggttt cagccactgg cgttcctgtt cgatttctga accgcccagg
     7381 ttgagcatca tcaccacgaa cacgaacagc accataatgg cacccgcgta gacgataatt
     7441 tccagcgcac cggcgaagta agcgcctagt gagaagaaca ccccggaaat cgccagcagc
     7501 gaaataatca ggtacagcag tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc
     7561 gcaagtatgg ctatcaggcc acagatataa aaagcgaact ccattgcccc tctccttacg
     7621 gtaacaggct cttgacgtcg ataggcttgg cttcgttctc tgcttcgccc ttatctttgc
     7681 cgtcgattgc catacctgcc atccggtaga agttatattc cgggtatttg cccggaccgg
     7741 agatcagcag atcctctttc tcgtaaacca gatcctggcg cttgtattcc cccatttcga
     7801 aatccggggt taactgaatc gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa
     7861 tgcagcgtga gaagttaatg cggaaaaatt ccggatacca gcgaccgtct ttggtttctg
     7921 ctttttgcag cgagatacag ccgaccgggc aggctaccgc acagaggtta caggctacgc
     7981 aacgctcttc gccgtccggg tcgcgggtca gaacgatacg accacgataa cggggcggca
     8041 gatagaccgg ctcttccggg tacattcgcg tttcgcgttt ggcgaacgcg tgcaggccga
     8101 tcatccagat actacgaacc tgggtgccga aacctactaa caattctttt aaggtcatgg
     8161 tcttattgcc ccttattgcg cctgccagag aatgacagcc gccgttacca gcaagttgat
     8221 cagcgtcagc ggcaggcaga ttttccagcc gaaggacatt acccggtcat aacgcggacg
     8281 cggtaacgac gcacgaatca aaatgaacat catcataaag aacgcggttt tcagcgcgaa
     8341 ccagatgaat ggcggtaaca acgggccttg ccagccaccg aagaacagcg tcaccatcaa
     8401 tgcagagatg gtcacaatcc cgatgtactc acccacgaag aacagaccga acttcatacc
     8461 ggaatattca atgtggtaac catccgccag ttcctgctcg gcttccggct ggtcaaacgg
     8521 gtgacggtga cacaccgcca cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg
     8581 gatgacgttc cacacatgcg cctggctgtt gacgatgtcg gtcatgttga atgaaccggc
     8641 ctgcgccacc acgcccatca gggaaagccc gaggaacact tcgtagctca gggtctgcgc
     8701 agaagcacgc atcgcaccca gcagcgagta tttgttgtta cttgaccagc ccgcaaacag
     8761 caccgcgtaa accgccagac ctgccatcat caggaagaac aaaatcccga tgttcaggtc
     8821 ggcaaccacc caacccggac tgactggcac aattgcaaag gccagcagca gcgaggtaaa
     8881 ggcaatcatc ggtgccaggg taaagatgac gcgatccgag aatttcggga tccagtcttc
     8941 tttaaagaac attttgatca tgtccgcaac cagctggagc gaaccgcccc agccaacacg
     9001 gttaggtcca taacggttct ggaacagacc cagcaggcga cgttcgccaa agctcatgaa
     9061 tgccccacag gtgacaacca ccagcaggat caccaccgct ttgaggatgg tcagcaggat
     9121 ctcaatcagt tccggtgata tccaactcat tgttgtgcct ccttgagatc ctcaagatgc
     9181 gcgccagcca gcaccggagc aatgccggac atacccatcg gcaagcccac ctgccctgcc
     9241 gtcagtcctt cggcgatttc aaccggcagc gtgaccgtgt tgccatcgta actaaaggag
     9301 acgcgtgtac ctgcgttcac acccaacttc gcggcatccg ctgggttgag tttgatgtac
     9361 ggctgcggca tacggctctg gaagaccgga gcacgctgtg acaattcatc gctgccaaac
     9421 aggtggtagt acggcgcgat acgccatttc ccgtcctgcg gctggaagcg tgccggtacg
     9481 ctggtgaagt aatccagacc gttttcgctg gtttcaaaca gacgcacgcc cggatcgcca
     9541 aagcgcagtt tgccgcccac ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag
     9601 cccggcgccc aggcaaacgg cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc
     9661 atcgagaagg tgaacatggt gtcaatatcc tgcggctgac gcggctcatg aacgctgata
     9721 ttggcgcgca tggcggtacg accgctgtaa cggtgcggtt cacgggccag tttctgccca
     9781 cgaatacgga atgtcgcatc cggcgcagca tctttgatac ccgccagttc tgggattttc
     9841 gccacaacag cgtcaattac atggtcgagc tgcgtccagt ccacttcacg gctcagcaag
     9901 gtgctgtgca gcgagtgcag ccagcgccag ctttccagca tgacagtttt gctgtcgtaa
     9961 taagccggat cgtaaacctg gaagaaacgt tgggcgcgac cttcattatt aattaccgta
    10021 ccgtcgcttt cagcaaagct ggcagaggaa agcaccagat gggcgttttc catgatcgct
    10081 gtgcgttgat gatcaaccac catcaccagc ggcgctttag ccagcgcagc attcacgcgg
    10141 gtagcagaag cgtaacgatg cagatcgttt tccaacacca ccaccgcgtc ggcgcgtccg
    10201 gtttccagtt cggttaacgc ttcttcaagc gaaccgccac ccataatgcc cagccccatg
    10261 ctgttgacgg aacgggcaat catggtgata ccaacgtcag cgccgcgacc tttcagggct
    10321 ttcgcgacgt tagccgcagc ctgaatcacc tctatgctac cggcgttcgt cccggagata
    10381 atcaacggtt tcttcgcacc tgccagtgcc tgcacgatga cgtcgatttt gttttgcagc
    10441 tcaggttcga taccgtcaac cgctggtgcg gagttatcca gcgcatgggc gatggcaaaa
    10501 cctaaacgcg cctgatcttc aaccggtgcg cggtaagtcc acgccgcgat atcatccaga
    10561 cgagtgtcat caacgttggt aacaaacagc ggatgcttcg cacgttgacc gatgttgagg
    10621 attgccgcaa tctgccagtc agccactttc tgtgctgcag ccatttcgcg cgctttacct
    10681 ttcaccgcct gacgcactgc cagtgcgacg cgcgcgccgg tctgggtaac gtcttcgccc
    10741 agcaccagta ccgcatcgta agattcgatt tcgcgcagag ccggagtata aatgccgcct
    10801 tcacgcagca ctttcagcgc cagttgcaga cgttcctgct caccgtgagc gataccggtg
    10861 tagaagtttt cttcgcccac cagttcacgc agcgcaaagt tgctttccac gctggcacgc
    10921 ggagaaccaa taccgatcac tttcttcgac tgacgcagaa tatctgccgc gccctgcatt
    10981 gcctgttcgg cgttgagggt aatgaaatca tcgccacgac gctgtactgg ctgacgcgga
    11041 cgatccttca ggttgacgta accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg
    11101 tttaccgtac cgttgtaacg gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg
    11161 ctgatgttac agccgatgga acattgctgg cagatgctcg gcgcaaactg catatcccat
    11221 ttacggttgt aacgctcgga gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg
    11281 accaggttac cggaaaattc gctttccagc gtgccgtctt ccgggcgacc gaagtagacg
    11341 ttgtcgtgcg cgccataaac gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc
    11401 acacagcggt agcaggcgat gcagcggttc atttcgtgag agatgaatgg ccccaaatcc
    11461 tgattacggt gggtacgttt ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg
    11521 gtcatatcct gaagatggca gttaccgcct tcttcacata ccggacagtc gtgcgggtgg
    11581 ttggtcatta accactcgac cacactttca cggaactgtt tcgcttcttc gtcgtcaatg
    11641 gaaataaagg tgccatcgga agccggtgtc atacaggaca tcaccaggcg accacgcgtg
    11701 tcttccgcgt tttggtattg cttcaccgca cactggcggc aagcaccgac gcttcccagc
    11761 gccggatgcc agcaaaagta aggaatatca aggcccagag acagacaagc ttccagcagg
    11821 ttgtccgctc cgttgacctc gtattctttg ccgtctacat gaattgtagc cattagcatg
    11881 cttccagttt tctcggtcag ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt
    11941 cagcaggttc ggctgaatcc cattaatcaa atgggtattg ctgaacggct gtttgattcc
    12001 cgcctcaaat tcttcgcgga aatatttgat ggcgctctgt aacggctcca ctgcaccagg
    12061 tgcgtgggca cagaaagttt tacccgggcc taagaatcga cacagttgct caagtgtttc
    12121 gatatcgccc ggctggcctt cgccacgctc cagcgcacgc agaattttca cgctccacgg
    12181 cagaccgtcg cggcacggcg tacaccagcc gcaggactca cgggcgaaaa actcttccag
    12241 gttacgcacc agcgacacca tgttgatctc atggtcaacc gccatcgcca gcgccgtacc
    12301 cagacggctg cccgctttac cgatactttc gaattccatc ggcagatcaa ggtgcgcttc
    12361 ggtcaggaag tcagtccccg cgccgcctgg ctgccaggct ttaaatttca gaccatcacg
    12421 cataccaccg gcgtaatctt cgaggatctc gcgtgcggtg gtgccgaacg gcagttccca
    12481 cagtcccgga tttttcaccc gaccggagaa gcccatcagc ttggtgccag catctttact
    12541 tttcgagatg ttctgatacc actccacgcc gttagcgagg atcgccggaa cgttacacag
    12601 ggtttcgacg ttgttgacac aggtcggttt gccccatacg ccggaggttg ccgggaaggg
    12661 gggtttcgag cgtgggttag cacgacgccc ttccagggag ttgattaacg ctgtttcttc
    12721 cccgcagatg tagcgccctg ccccggtatg gacgaacagt tcgaagtcga aacctgttcc
    12781 cataatgttt ttgccaagca agcccgcttc agtggcttcg gcaatggcgc ggcgcagatt
    12841 tactgccgct tcgatatatt cgccacgcag gaagatgtag ccacggtaag ctttcagcgc
    12901 aaacgcggag atgagcatac cttccaccag caggtgcggc agttgctcca tcaacaggcg
    12961 gtctttatag gtgcccggct ccatttcatc ggcgttacac agcaggtaac ggatgttcat
    13021 ggattcgtct ttcggcatca ggctccactt caggccagtc gaaaagcccg cgccgccgcg
    13081 ccctttcaga ccagcgtctt ttacctgatt aacgatttcg tccggagaca gcccggtcag
    13141 cgccttacgc gccccttcgt aaccgttttt gctgcggtat tcgtccagcc acactggctg
    13201 tttgtcatcg cgcagacgcc aggtcagcgg atgcgtttcg ggagtacgga taatgttttt
    13261 catttatacc gctccagcag ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc
    13321 tcatcgatca tcatgtttgg ccctttatca cagttcccca ggcagcaagt tggcagcagc
    13381 gtaaagcggc catcaaatgt cgtttgccct ggtttgatgt tcagcttttt ctcgagcgcc
    13441 gcctgaatac cctgataccc gttgatatga cagaccacgc tgtcgcaata acggatcaca
    13501 tggcgaccaa ccggctggcg gaagatctga ctgtagaacg ttgccacacc ttcgacgtcg
    13561 cttgccggaa tacccagcac atcggcgatc gcgtggatcg caccatccgg cacccagcca
    13621 cgctgcttct gaacgatttt cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg
    13681 tgcatctcgt gctcaatcgc ttcacgctct gccgcactca gctcaaaagc ctcggtttgt
    13741 ggttgttgat tctcgtgcat aattagcggt ccacatctga cataacaaaa tcgatactgc
    13801 ccagataaac aatcaggtca gacaccaggc tgccgcggat cgccgccgga atttgctgca
    13861 aatgcgcata actcggcgtg cggatacgag tgcgataact catggtgcta ccgtcgctgg
    13921 tcaggtagta actgttgatc cccttggtcg cctcaatcat ctggaaagat tcattggcag
    13981 gcatcaccgg cccccacgac acttgcagga agtgggtgat cagggtttcg atatgttgca
    14041 gcgtgcgctc tttcggcggc ggcgtggtca gcgggtgatc cgctttgaac gggccttccg
    14101 gcatgttgtt gaggcactgc tcaagaatgc gcagactctg gcgcagctct tccactttca
    14161 gcattacgcg ggtgtagcag tcagaaacac caccaccaac cgggatttca aagtcgaagt
    14221 tttcatagcc agaataagga cgcgccttac gcacgtcgaa gtcgatcccg gtagcacgca
    14281 ggcccgcgcc agtggtgccc cactccagcg cctctttcgc gccataggcg gcaacgccct
    14341 gggaacgacc tttcagaatg gtattttgca gcgccgcttt ctcgtaagac gccagacgtt
    14401 tcggcatcca gtcgaggaac tcacgcagca gacgatccca gccgcgcggc aggtcgtgcg
    14461 ctacgccgcc aatacggaac cacgccgggt gcatacggaa acccgtgatc gcttccacca
    14521 gatcgtaaat tttctgacga tcggtaaagg cgaagaacac gggcgtcatt gcgccgacgt
    14581 cctgaataaa ggtcgagatg tacagcaggt ggctgttgat acggaacagt tcggagagca
    14641 taacgcgaat gacgttaacg cgatccggca cggtgatccc ggccagtttc tctaccgcca
    14701 gcacgtaagg catttcgtta acgcagccgc cgaggtattc aatacggtcg gtatacggaa
    14761 tgtagctgtg ccaggactgg cgctcgccca ttttctccgc accacggtgg tggtagccga
    14821 tgtctggtac gcagtcgaca atctcttcgc cgtcgagttg taaaacgata cggaaagccc
    14881 cgtgcgccga cgggtggttc ggaccgaggt tgaggaacat gaagtcctca ttttcggtgc
    14941 cgcgcttcat cccccactct tccggtttga aggtcagggc ttccatctcc agatcctgtt
    15001 tggctttggt cagctcaaac ggcgagaatt cggtagcgcg tgccgggtaa tctttacgca
    15061 gcgggtgacc tttccaggtt tgcggcatca tgatgcggcg caggttcggg tgaccgtcga
    15121 aagtaatgcc aaacagatcc caggtttcac gctcatacca gttagcgttc gggaacagtt
    15181 tggtgaaggt cggtacgtgc aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc
    15241 ggttacgatc gatagaaatc agatggtaga aaacggaaaa atccgcggca ggtaaccctt
    15301 cgcggtgtgt gcgcagacgt tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt
    15361 tcggcagttt ctttaagaaa tcgccaactt ccagtaattg ttcacgcttg atccacacaa
    15421 cgggaacccc ggtgcgagtc gcctgaacag taaaggcatc cggcccaaaa cggttgcgca
    15481 gttcgccaat taccggatca tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg
    15541 ttaagtcggt catattgttc accattgcaa atggtccgtg gtgactgtcg gcgcagtaag
    15601 cttcgcgaaa tagatttagg aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt
    15661 aaatctcgtc aggtgtacgc aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc
    15721 gctctgattg catattggcg cgataaacgc cctgatcgcc aaccacccag gagagcggac
    15781 gacgttcttt gccgatagat tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac
    15841 gcggcgggca gcccgggata tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa
    15901 cggaataaat atcgtacata ccaccagagt tggcacaggc acccattgag ataacccatt
    15961 ttggttccag catctggtca tacagacgct gaataaccgg tgccattttg gtaaagcagg
    16021 ttcctgcaac caccatcagg tcagcctgac gcggcgaagc acgcaatact tctgcgccaa
    16081 aacgcgccac gtcatgcacc gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa
    16141 gaccgaagtt atacggccaa attgagtttt tacgacccca gttaaccatg tcattgagct
    16201 tgcccataaa cacgtttttg ttaacttctt gctccagagg gtcggttacg atctcctgct
    16261 tttgcagggg gtaacggtcg ttctcaccgt tgggatctat gcgggtgagc gtataatcca
    16321 tcttaatgcc tcgcggttag cgttgacgat tagcgatact gttcgtttcc gggttcatac
    16381 gctcgcggcg tgaacgcgcg ggcgtccagt ccagcgcgcc aatacgcacc agataaacca
    16441 gacctgccag taacacaaaa ataaaaattg cagcttccac aaagcctacc cagccgcttt
    16501 cgcggataga ggttgaccat gcgaacagat acagcgcttc aacgtcgaag ataacgaaga
    16561 acatggccac cagataaaac ttggcggaca ggcgtaagcg ggcggagccg accgagtcga
    16621 taccggattc aaacggcacg tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac
    16681 cgcctaccag catcaggcaa cacaggccaa tggcaacgat aagaaagata gcgaatgccc
    16741 agtgatgagc gatgacttca gtggatgttg acatactcat tgcttactca tcaaaagtag
    16801 cgccagattc actgctcttc acggcagatg gacgccacat cgattcatgg ggaggaataa
    16861 aaaaaacctt acaataactg tagaaattct tttatacagc taattgatgt ggtcttttac
    16921 tcctttctat aaccttttgt caactttaac aaaagcttct tcacattagt ttacataata
    16981 tcaacaccat tagcatttaa tgccctttca ccccagatcc ttgacgactc caggataatt
    17041 agatgttgtt gaatcgtgtc cgttgtgaag caatggaaaa aatacgggtc tattttgaca
    17101 ggaatttgtg tcgattcctc cccccaaaag agagtatttt cttgatctgt gacacgcttt
    17161 tgttattcca taacaaaaac gcagcaacaa atttacgtat tttttaacat cattgtagca
    17221 ggtgattttt ttcaggcgat tatttgtgca ttcgggacgt gaatctctgg tgattgaaaa
    17281 ataaacagtt ttgtacgttc tgcactatgc gaggaaggat ttttactaaa aaaaagccgc
    17341 tggggtttaa aacaccccca gcggctcgtt ttttacacta ttgtctcagg aattatctat
    17401 cgtccgtcga ttactcgata tccctttcaa tcaacaatga atcatcccct tccggagcag
    17461 acattgggct gtattgccac ggattatggt agctctccat cgcctgatag atcacctggg
    17521 ccagctcatt attactggac gggtcatagc acagcaaata ttcggtatca ggtagcggcg
    17581 gtaagccatc tactccactc agcacacgca ggtccgggct catcatctca accggccttg
    17641 ccgtcacgcc aagaccggct ttcactgccg cacgaacggc cggaagcgtc gaggcgacat
    17701 aagccagtcg ccatggaata tctactttat taagcgtcgc cagcaccata tcgcgaaacg
    17761 gacttggatc atccagcaat acaagaggga tcggctcgcc tttttgcaat acgtattccg
    17821 ctgcgcagta ccagtgtgtt ggcgaggtac gcaggttcaa tgccttaaac gctgaaggac
    17881 gatgggtggt taccatcaaa tccacttcct gggattccag catttctgcc atataggcat
    17941 tacgtttaac gcggacatcc agcgctaatt tcggataaac cgaactcacg cgatttaaca
    18001 ggaaaggtaa gatcgtatcg gcagattcat ctgaagcacc gatagttaac acgccctgaa
    18061 gattactgaa cattaatgat gagcaggcct catcattaaa acgcaggatt ttcctggcgt
    18121 aaccaagaag ttgaatgcca tgttcagtta acagtttgtt gcgaccgtga cgagcgaaca
    18181 gttctttccc aacgagttgt tccagacgct gcatttgctg acttacggcg gactgagtac
    18241 gacacacagc ggcagctgcg gcagcaaaag tgttcagatc ggcaacagca acaaatgttc
    18301 tcagcagatc gaggtcgagg ttaattatcg gacgatttgc acttatcata tattatcact
    18361 tactggcggc tcatactgag ctggttaatg ctgtgcacac acaaacaagc aattccattt
    18421 gtaatgtgcc tccctggcag tttcatccgg aacccggacg aaagtaaaaa tgcatatgag
    18481 ttgcactaaa aaagcgactc acattgttcc gttataatgc ctgaagtaga tcacagaata
    18541 tatcttcagg gatcgcatat ctattaagtt actcactctt ttctatttat gacatgcgcg
    18601 tgtttgtata aatgtaaatg tgagtccttg ttccactctc gtgcagcatc gctggtcata
    18661 cgcgaacacg tacaaacagc aatggtgaag cggcatcaag agcagggatc cgtcatttat
    18721 gcgagcattt taccccaaaa accttttatt tataagggtc attgcgaatt atctgatgca
    18781 aagttatgtt atgttaggca aagtaatctt ctattattaa taagcacatc aaaacctttt
    18841 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
    18901 tttcacacct tacccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
    18961 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
    19021 attatcttac agataaaaaa accagacttg caattaagaa ttagaacaat tcactatata
    19081 acattgcatg taaagcatat aagcctcatt atttgtcatt attaagttat taacagcaca
    19141 atcgagcctt cccctctggc aaaatcttat tctgcagacc ttcaaaacac cgtcctgggg
    19201 gagtacattg ttctaagctg acttccacgg cagggagtgg cgataacagc aaaaaaggtc
    19261 aagattcatg tcccccattg aaaaatccag caaattagag aatgtctgtt atgacatccg
    19321 tggtccggtg ctgaaagaag caaaacgcct ggaagaagaa ggtaacaagg tactgaaact
    19381 gaacatcggc aacccagccc cattcggttt tgacgcgcca gatgaaatcc tcgttgacgt
    19441 gatacgcaac ctgcctaccg ctcaagggta ttgcgattcc aaagggcttt actccgcgcg
    19501 taaagccatc atgcagcact accaggctcg tggcatgcgt gatgttaccg tggaagatat
    19561 ttacatcggc aatggtgtat cggagcttat cgttcaggca atgcaggcat tgctgaacag
    19621 cggggacgaa atgttggttc ctgcaccaga ttacccactc tggaccgcgg cggtttcgct
    19681 ttccagcggt aaagcggtgc attatctttg cgatgaatcc tctgactggt tcccggacct
    19741 cgatgatatt cgcgctaaaa ttacgcctcg tacgcgtggg attgttatta tcaacccaaa
    19801 taacccaacc ggcgcggtat attccaaaga gcttttaatg gagattgtgg agattgcacg
    19861 tcagcataat ctcattatct tcgccgatga aatttatgac aaaattctct acgacgatgc
    19921 tgagcatcac tcaattgcgc cgctggcacc tgacctgctg accattacct ttaacggact
    19981 gtcgaaaacg taccgcgttg caggcttccg tcaggggtgg atggtgttga acgggccgaa
    20041 aaaacacgcc aaaggctaca tcgaaggtct ggaaatgctg gcttcaatgc gcctgtgtgc
    20101 taacgttcct gcgcaacacg ccattcagac cgcgctgggt ggttatcaga gcatcagtga
    20161 atttattacc cctggcggtc gtctttatga gcagcgtaac cgcgcgtggg aactgatcaa
    20221 cgatattccg ggcgtttcct gcgtgaaacc tcgtggtgcg ctgtatatgt tcccgaaaat
    20281 cgacgccaaa cgctttaaca ttcatgacga tcagaaaatg gtgctggatt tcctgttgca
    20341 ggaaaaagtt ctgttggtgc aagggacggc attcaactgg ccgtggccgg atcacttccg
    20401 cattgtcacg ctaccgcgtg tcgatgatat cgagctgtct ttgagcaagt tcgcgcgttt
    20461 cctttctggt tatcatcagc tgtaatctta atttcactgc cggagattgc atccggcagt
    20521 gttatcccgc cacaatgacc tgatgatgtc atcatacgta aggtcactat gaaacagagc
    20581 catttttttg cccatctctc ccgcctgaaa ctcattaacc gctggccgct aatgcgcaac
    20641 gtgcggacgg aaaatgtgtc cgaacacagt ttgcaggtag cgatggtcgc ccatgcgctg
    20701 gcagctatca aaaatcggaa atttggcggt aatgtcaacg ccgaacgtat cgctttactg
    20761 gcgatgtacc acgatgcctc agaagtgctc accggcgatc tccctactcc ggtgaaatac
    20821 ttcaatgcgc aaatcgctca ggagtacaag gctattgaaa aaatcgctca gcagaaactg
    20881 gtcgatatgg ttccggaaga actgcgggat atctttgcgc cgttaattga cgagcatgca
    20941 tatagcgatg aagaaaaatc gctggtgaaa caggcagatg cactgtgtgc atatctgaaa
    21001 tgtctggaag aactcgcggc cggaaataat gaattcttgc tggcaaaaac gcgactggaa
    21061 gcgacgcttg aagcgcgtcg cagccaggag atggactact tcatggaagt atttgttccc
    21121 agcttccatc tttcgctcga tgagattagc caggattcac cgctgtaagc agccggagtc
    21181 tgcgtcgcat cagtcaataa gcgccggatg cgacatcagg ctcttgtcaa aacggaaaca
    21241 gcatcgggat catcaccaca caaaccgcca tcacgataat ggtgaacggt acccccaact
    21301 tcacaaagtc actgaagctg taatttcccg gacctaaaac cagggtgtta acaggtgaag
    21361 acaccggtgt cataaaggcg gcggatgctg ccatcgccac gaccatcgca aatggataag
    21421 gcgacactcc catcgttttg gcagcagcca gcgcaatcgg agccatcaac accgccgtcg
    21481 cggtattaga gataaacagc ccaataaccg ccgacaagac aaacaaacac cccagcatca
    21541 tatgtggccc ataaccgccg ccaatatcca tcagcccttt caccgccagc gcgacacctc
    21601 ccgttttctg taatgccaca gcaaacggca tcatcccaac ggtcaaaata atgctcggcc
    21661 agtgaatgga tttataggcg ctttcagcat ctatacagcg gaatttcccc atcagcaggc
    21721 aggcgatgat agcggcaaca ggattaggaa tttcatttgt cagcattaac gccaccatta
    21781 gcaccagaca gaaaatggca tggggtgcct ggctgtgcgc gggcgatgct tcactcacct
    21841 cttccggtaa gttcagcgct acgaagtcgc ggccctgttt ggccagcata ccgatcagtt
    21901 tccagttacc cacaaccagg atgatatcgc ccagcagcag aggctcatcc gccagcgaac
    21961 cttccagcgc cacgccattg cgcttgagcc ccaccacatt cagtccgtag cgggtacgaa
    22021 aaccaatttc gcgcaccgat ttaccaatca gttctgactc aggaattaat gaaatctctg
    22081 ccatgcccac atcaagggcc tggtcagaaa aatactcgcc gcgcagtacc atcggctcca
    22141 gcaattgctc actacaaaat tgccggagat cgacatcagc cgcagacata tcaataagca
    22201 aaacgtcacg cgcgcgaaat tcagaaaccc cattaacgtt cacgataacg cgacgaaaac
    22261 gccgccagcg ttcaacaccg atgacgttag cgccataacg ctcacgtaat ttgagatcat
    22321 ccagccgttg acctatcatt ggcgatccgg ggcgaatagc cagacgtcgc gcacgcccgg
    22381 tcagtcgata ttcacggata agatcgcgaa aggttcgacg cgtccagcct tcgcgctgcg
    22441 gggtctgggt atcccctttc agcatgaaac gcatcactaa catatacaaa atacccagca
    22501 ccagcacgac caggccaata ggtgttacgc taaagaaact gaagccgtga tagccttcac
    22561 gcagcaattc actgttgact accaggttcg gcggcgtcgc caccagcgtc atcatgccgc
    22621 taatcagccc ggcaaaactt aacggcatca tcagacgtga cggcgaagtt tgcatacgca
    22681 tggcaacgct taacaccacg ggaataaaga tagcgacaac gctggttgaa ctcataaacg
    22741 cgccaagccc cgcgacggtc agcatcaaca aaaccaacat tttgatttca ctgttgcccg
    22801 ccactttgac cagccatgtt cccattacgg tggcaacacc ggtacggacc aaaccatcgc
    22861 caataataaa caaggcggca atcaggacaa cgttaggatc agaaaagccg gaaaatactt
    22921 cggggaccgt cagcgttccg cttaatgcaa acgcgacaat aacaaacaaa gcgaccgcat
    22981 ccatacgcac tctgcccgtc gcaaacaaga cgatggcaac ggccagtaat gaaagaaccc
    23041 aaatcaattc accgttcaca acttatcctt gttaattgag ggggatgact tgattctgcc
    23101 ataaaaaagc cccgacgaga cggggctaaa tcatgatcag gtgtttcact gaataataac
    23161 atcgccattt ggctgtttgg tcacagtaat ttgctccaga ctgtggagga ccaaatcgac
    23221 ctcattcagg cgcggggtat ctgccggagc gttaaccgca atgacatggc aacccgccgc
    23281 caggccagaa agcacgccag cgggagcatc ttccaccacc acacactcct gcggcgcaag
    23341 ccccagcagc tgcgcaccta acagatacgc atcaggttct ggttttccgc gcttcactcg
    23401 ctcagcggtt acaaacacct ctggtgcggg aagccccgct attttatggc gcgctcgcgc
    23461 taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat tcaaatgatt
    23521 gagtaaggcg attgccccag gaagcgcggt aataccttcg gtttccgtgg cctcgatttg
    23581 ctccagacgc gtaaactcgg cggcaatatc agcctcggat ttgcccgcca taaaatggcg
    23641 caaagaggtg atcgcctgtt taccgtgaat gaaagccagc acctcttccg gcgctaaccc
    23701 atgacgtctg gcccagttgc tccacgcccg ttctaccgca ggcagggaat ccaccagcgt
    23761 tccatcaaga tcaaacagaa aacctttgca ccgcacgcgg gcctcctcag gcgttgataa
    23821 tttgattaat ttcgttggcg ctcaaatggt actgacgcgg gcaggcatgc cacacattaa
    23881 gcatgcgctg atatttttcc cacattggcg tctgggcgtt aaaaccatga gtcccagcgt
    23941 caaaatgggt atagcgccct tccacattaa ccataaagcg gacataaccg aggtaacgtg
    24001 cttcagtggc ggcgtcaaag ccgaggaagg tgacacgacg ttcatcgatg gattgctggt
    24061 cctgcaaatt agaccaggaa acatacaacg catgatacat atccataatg tcgatgatag
    24121 tgcggcaggt ttcttctttc agctcgccaa actcgcgatc caattcacgc atctgtaatc
    24181 cgtaaccacg ctcaataatt gtttgcaggc gacggtaacg ttcagcattt gccggatcga
    24241 gcatagtcat catcttgtac tgattagaca aaataagacg ttgcgcgttg gtcatttcca
    24301 ttgttgactc ctgtatcact ctactacggt gaaaaaaaag aaggctgagt atgccttctt
    24361 ttatatgcgt aatcaggggt caattacaaa tcatcaagga aagttttatc cagttgtttg
    24421 aaggcgcgct taagcgtgtc agctaatgcc tggtaatcag gcttgccttc aacgggtgcc
    24481 aacacctgtc cagactcctg caatttaccg cgaacttcat aaaaccagtt aaggattgca
    24541 gggggtaatg gcgttacaga acgcttgccc agccaccaca atccctgcat gggtaaactt
    24601 aaggcgaaca gtgcagtggc aactgccggc ccaagcagac cgcccagggc aatctgccag
    24661 cagagagtaa atacggcgat cggcggcata aaacggatcg cataacgcgt catcttgata
    24721 acgcgatttt cgacaaagac cggagcaagg cgtttttcca gtggccacgt ctttgagtaa
    24781 tgctgtccgc ggcgaaacaa gctaaaaaaa ttaacagaac gattatccgg cgttgacatg
    24841 cttcacctca acttcacata taaagattca aaaatttgtg caaattcaca actcagcggg
    24901 acaacgttca aaacattttg tcttccatac ccactatcag gtatccttta gcagcctgaa
    24961 ggcctaagta gtacatattc attgagtcgt caaattcata tacattatgc cattggctga
    25021 aaattacgca aaatggcata gactcaagat atttcttcca tcatgcaaaa aaaatttgca
    25081 gtgcatgatg ttaatcataa atgtcggtgt catcatgcgc tacgctctat ggctccctga
    25141 cgttttttta gccacgtatc aattataggt acttccatgt cgagtaagtt agtactggtt
    25201 ctgaactgcg gtagttcttc actgaaattt gccatcatcg atgcagtaaa tggtgaagag
    25261 tacctttctg gtttagccga atgtttccac ctgcccgaag cacgtatcaa atggaaaatg
    25321 gacggcaata aacaggaagc ggctttaggt gcaggcgccg ctcacagcga agcgctcaac
    25381 tttatcgtta atactattct ggcacaaaaa ccagaactgt ctgcgcagct gactgctatc
    25441 ggtcaccgta tcgtacacgg cggcgaaaag tataccagct ccgtagtgat cgatgagtct
    25501 gttattcagg gtatcaaaga tgcagcttct tttgcaccgc tgcacaaccc ggctcacctg
    25561 atcggtatcg aagaagctct gaaatctttc ccacagctga aagacaaaaa cgttgctgta
    25621 tttgacaccg cgttccacca gactatgccg gaagagtctt acctctacgc cctgccgtac
    25681 aacctgtaca aagagcacgg catccgtcgt tacggcgcgc acggcaccag ccacttctat
    25741 gtaacccagg aagcggcaaa aatgctgaac aaaccggtag aagaactgaa catcatcacc
    25801 tgccacctgg gcaacggtgg ttccgtttct gctatccgca acggtaaatg cgttgacacc
    25861 tctatgggtc tgaccccgct ggaaggtctg gtcatgggta cccgttctgg tgatatcgat
    25921 ccggcgatca tcttccacct gcacgacacc ctgggcatga gcgttgacgc aatcaacaaa
    25981 ctgctgacca aagagtctgg cctgctgggt ctgaccgaag tgaccagcga ctgccgctat
    26041 gttgaagaca actacgcgac gaaagaagac gcgaagcgcg caatggacgt ttactgccac
    26101 cgcctggcga aatacatcgg tgcctacact gcgctgatgg atggtcgtct ggacgctgtt
    26161 gcattcactg gtggtatcgg tgaaaatgcc gcgatggttc gtgaactgtc tctgggcaaa
    26221 ctgggcgtgc tgggctttga agttgatcat gaacgcaacc tggctgcacg tttcggcaaa
    26281 tctggtttca tcaacaaaga aggtacccgt cctgcggtgg ttatcccaac caacgaagaa
    26341 ctggttatcg cgcaagacgc gagccgcctg actgcctgat ttcacaccgc cagctcagct
    26401 ggcggtgctg ttttgtaacc cgccaaatcg gcggtaacga aagaggataa accgtgtccc
    26461 gtattattat gctgatccct accggaacca gcgtcggtct gaccagcgtc agccttggcg
    26521 tgatccgtgc aatggaacgc aaaggcgttc gtctgagcgt tttcaaacct atcgctcagc
    26581 cgcgtaccgg tggcgatgcg cccgatcaga ctacgactat cgtgcgtgcg aactcttcca
    26641 ccacgacggc cgctgaaccg ctgaaaatga gctacgttga aggtctgctt tccagcaatc
    26701 agaaagatgt gctgatggaa gagatcgtcg caaactacca cgctaacacc aaagacgctg
    26761 aagtcgttct ggttgaaggt ctggtcccga cacgtaagca ccagtttgcc cagtctctga
    26821 actacgaaat cgctaaaacg ctgaatgcgg aaatcgtctt cgttatgtct cagggcactg
    26881 acaccccgga acagctgaaa gagcgtatcg aactgacccg caacagcttc ggtggtgcca
    26941 aaaacaccaa catcaccggc gttatcgtta acaaactgaa cgcaccggtt gatgaacagg
    27001 gtcgtactcg cccggatctg tccgagattt tcgacgactc ttccaaagct aaagtaaaca
    27061 atgttgatcc ggcgaagctg caagaatcta gcccgctgcc ggttctcggc gctgtgccgt
    27121 ggagctttga cctgatcgcg actcgtgcga tcgatatggc tcgccacctg aatgcgacca
    27181 tcatcaacga aggcgacatc aatactcgcc gcgttaaatc cgtcactttc tgcgcacgca
    27241 gcattccgca catgctggag cacttccgtg ccggttctct gctggtgact tccgcagacc
    27301 gtcctgacgt gctggtggcc gcttgcctgg cagccatgaa cggcgtagaa atcggtgccc
    27361 tgctgctgac tggcggctac gaaatggacg cgcgcatttc taaactgtgc gaacgtgctt
    27421 tcgctaccgg cctgccggta tttatggtga acaccaacac ctggcagacc tctctgagcc
    27481 tgcagagctt caacctggaa gttccggttg acgatcacga acgtatcgag aaagttcagg
    27541 aatacgttgc taactacatc aacgctgact ggatcgaatc tctgactgcc acttctgagc
    27601 gcagccgtcg tctgtctccg ccagcgttcc gttatcagct gactgaactg gcgcgcaaag
    27661 cgggcaaacg tatcgttctg ccggaaggtg acgaaccgcg taccgttaaa gcagccgcta
    27721 tctgtgctga acgtggtatc gcaacttgcg tactgctggg taatccggca gagatcaacc
    27781 gtgttgcagc gtctcagggt gtagaactag gtgcaggcat tgaaatcgtt gatccagaag
    27841 tggttcgcga aagctatgtt ggtcgtctgg tcgagctgcg taagaacaaa ggcatgaccg
    27901 aaaccgttgc ccgcgaacag ctggaagaca acgtggtgct cggtacgctg atgctggaac
    27961 aggatgaagt tgatggtctg gtttccggtg ctgttcacac taccgcaaac accatccgtc
    28021 cgccgctgca gctgatcaaa actgcaccgg gcagctccct ggtatcttcc gtgttcttca
    28081 tgctgctgcc ggaacaggtt tacgtttacg gtgactgtgc gatcaacccg gatccgaccg
    28141 ctgaacagct ggcagaaatc gcgattcagt ccgctgattc cgctgcggcc ttcggtatcg
    28201 aaccgcgcgt tgctatgctc tcctactcca ccggtacttc tggtgcaggt agcgacgtag
    28261 aaaaagttcg cgaagcaact cgtctggcgc aggaaaaacg tcctgacctg atgatcgacg
    28321 gtccgctgca gtacgacgct gcggtaatgg ctgacgttgc gaaatccaaa gcgccgaact
    28381 ctccggttgc aggtcgcgct accgtgttca tcttcccgga tctgaacacc ggtaacacca
    28441 cctacaaagc ggtacagcgt tctgccgacc tgatctccat cgggccgatg ctgcagggta
    28501 tgcgcaaacc ggttaacgac ctgtcccgtg gcgcactggt tgacgatatc gtctacacca
    28561 tcgcgctgac tgcgattcag tctgcacagc agcagtaatc tcgtcatcat ccacagcttt
    28621 gcgctgcgga tatctgaacc ggaaataatc actatttccg gttttttatt ctcttaattt
    28681 gcattaatcc tttctgatta tcttgcttaa ctgcgctgca tcaatgaatt gcgccatctc
    28741 actttgcata cttaccactt tgttttgtgc aagggaatat ttgcgctatg tccgcaatca
    28801 ctgaatccaa accaacaaga agatgggcaa tgcccgatac gttggtgatt atcttttttg
    28861 ttgctatttt aaccagtctt gccacctggg tagttccggt ggggatgttt gacagtcagg
    28921 aagtgcagta tcaggttgat ggtcaaacaa aaacacgcaa agtcgtagat ccacactcat
    28981 ttcgcattct gactaacgaa gcaggcgaac ctgagtatca ccgcgtacag ctgttcacga
    29041 cgggcgatga acgcccgggc ctgatgaact tcccgtttga agggttaacc tcaggatcga
    29101 aatacgggac agccgttggc atcatcatgt ttatgctggt gattggcggc gcgtttggca
    29161 ttgtgatgcg tacaggaacc attgataacg gtatcctggc gcttattcgc catacccgcg
    29221 ggaatgaaat tctctttatt cctgcgctgt ttattctgtt ttcacttggc ggcgcggtat
    29281 ttggtatggg agaagaggcc gtcgcctttg ccattatcat cgcaccgcta atggtccggc
    29341 tgggctatga cagtattacc accgtcctgg tgacttatat tgccacgcaa atcggttttg
    29401 ccagttcgtg gatgaacccg ttttgtgtgg ttgttgctca ggggattgcc ggcgttccgg
    29461 tgctttctgg ctccgggttg cgcatcgtgg tgtgggttat cgccactctg attggcctga
    29521 tctttaccat ggtgtacgcc tcacgagtga aaaagaatcc tcttctgtca cgcgtgcatg
    29581 agtccgaccg cttctttcgt gaaaagcagg cggatgttga acaacgtccg tttacctttg
    29641 gtgactggct ggtattgatt gtcctgacag ccgtaatggt ctgggtgatt tggggcgtga
    29701 tcgttaatgc ctggtttatt ccagaaattg ccagccagtt cttcaccatg ggtctggtga
    29761 ttggcatcat cggcgttgtt ttccgcctta acggcatgac ggttaatacc atggcttcat
    29821 cctttaccga aggggcgcga atgatgatcg cccctgccct gctggtgggt ttcgccaaag
    29881 ggattttgct gctggttggt aatggtgaag caggtaatgc cagcgtgtta aatactatcc
    29941 tcaacagcat tgccaatgcc attagcggtc tggacaacgc ggtcgcggcc tggtttatgt
    30001 tgctcttcca ggctgtattt aatttcttcg tgacgtccgg ttctggtcag gcggcattaa
    30061 ctatgccgtt actggcaccg cttggcgatc tggtcggtgt taaccgtcag gttaccgtgc
    30121 tggcgttcca gtttggtgat ggcttcagcc acatcattta cccaacctca gcttcgttaa
    30181 tggcaacgct cggtgtgtgc cgggtggact tccgtaactg gctgaaggtg ggcgcgaccc
    30241 tgcttggact gctgtttatt atgtccagcg tcgtagtgat cggcgctcag ttgatgggct
    30301 accactaaaa atgttaagag ccgcaattgc ggctcttttt tcattctgcc gtttcagtct
    30361 ctacggcttc atttttggca ttgcgcttca tccacaacgc cagcgctttc agcgagtctg
    30421 gagtgaactc atcgcagcgt gcggtgattt cttccggcgt cagccagcaa actccactga
    30481 cttcatcttc ctgtagggcg aagggaccgt gagagacgca actgaacaac gcgccccaga
    30541 cacggcaatt tttatcttcg aaatagaact gcccgtgctc ggcaaagggg acaccggcaa
    30601 tgcccaactc ttcttccgct tcgcgacgcg cggattccag cagttgctca tcggcctgaa
    30661 ctacaccgcc tgcggtcgca tctaacatgc cgggtaaaaa gtcttttgtc tcggtacgac
    30721 gctggaccag aattttgccc atgccatcat gcacgacgat gtaagttgca cgatgacgca
    30781 gacactgtgc ccgcatttgt tcccggctgg cttgtgcaat gacttcgttc tcttcattga
    30841 caatatccac ccattcagta cttgccaaac gacgctgttc cattatcagg aaaccttctt
    30901 tttctggcgc tcttacggcg cattttgagt tgtgggtaaa ttacggatta atcgcgacct
    30961 gcgcaatgat actttgatca ttgagtgcga ttacactaag tacgtcatta tccagcatgc
    31021 cataacccgc cggattaccg cctttcggaa tactcaccga gccggggttg aagtggaaaa
    31081 tctccccccg ctgttctgcc actggcagat gggtatgacc gtacaccagc acatcgttct
    31141 ggtttaaagc aggtagattt tccgggccaa aaagatgacc atgcgtcaaa aacagacgtt
    31201 gtttttccag taatacctgt tgccacggcg cggttatcgg gaaatgcagc atcatttgat
    31261 ccacttcgct gtcgcagttg ccacgcacag cgataacctt atgtgccact tcattaagcc
    31321 gttcagcgac tttggctggc gcgtaaccct ccggtaaagc attacgcggg ccgtgattca
    31381 acacgtcgcc aaggatcacc agccactggg caccgctttg gacaaacaac tccacaacac
    31441 gttccgtcgc cggtaacgac ccatgaatgt ccgatgcaaa catcagtttc atcactcact
    31501 cctcgtcgaa aaaacgtaag ctatgatact ggattctgtc gttgctatca gccagattgc
    31561 ttcgccgaaa gtgcaataaa acgctggaca gacgctcgct gccactggaa tgtcgcataa
    31621 tccaccaggc gttctggcac ctcatcgcca tgtagtacca ggcggttaat cattagcgcc
    31681 agatcagtat cagcaatgca ccattcacca aataaattcg gctgacccag tgctaacaaa
    31741 tgttctgcca tcgcgaacag tttctctgca ctggcttttc cctcggccgt tagtggcgct
    31801 tttttcgccc ccgcaaagac aacatccgtc ggacgctctt cgcggatggg catcagatcg
    31861 ctgcgcagcc aggcctgaat ctgtcgtgca cgcgcacgat tttctaaatc aagcggataa
    31921 atacgttccc aggtcggtgg cgcaaatcga tcttccagat actccgcaat ggcagaagat
    31981 tcactcaact caaaatcatc gatttgtaat aacggcacac ggcgtgtctg accgtaacct
    32041 tgccacgtcg gctgcaaatg ttcaccgctg tcgaggtcga tggtcttgat atgaaacgac
    32101 aggccttttt cctgcaacgc cacccaggcg gataacacat aaggggagaa aaagtgggca
    32161 tctgaccaaa gcgtgatagc gggtttactc attatgtcct cggcaataaa gggggctggc
    32221 tatcaaacta tagagccttt ttgtcactgt cacgtgacga atactcacgt aagtcgccgc
    32281 gctttctata cttgtggtgt tcagatgatt aaacaaccgg agctgcaatg atcgatctct
    32341 atttcgcccc gacacccaat ggtcacaaaa ttacgctgtt tctcgaagaa gcagagcttg
    32401 attatcgctt gattaaggta gacttgggga aaggcggtca gtttcgtccg gaatttttgc
    32461 gcatttcgcc caacaacaaa attccggcaa ttgttgatca ttctcctgcc gatggcggcg
    32521 aaccgttaag cctctttgag tctgctgcca ttttgttgta tctggctgag aaaacaggac
    32581 tctttttgag tcatgaaacg cgtgagcgcg ccgccacatt acagtggtta ttctggcagg
    32641 taggcggact ggggccgatg cttgggcaaa atcatcattt taatcacgca gccccccaaa
    32701 ccattcctta cgctattgaa cgttatcagg ttgaaactca gcgtctttac catgtactaa
    32761 acaagcggct ggaaaactcg ccctggctgg gaggcgagaa ctacagcatt gcggatattg
    32821 cctgctggcc gtgggttaat gcctggactc gcctgcgaat tgacctcgca atgtatccgg
    32881 cagtcaagaa ctggcatgag cggatccgtt cgcgccctgc caccgggcag gcactgctaa
    32941 aagcacaact cggtgatgag cgttcggata gttaacagaa acaggttctc gtgtattatt
    33001 tcatcctaag taaaacaacg gagaacctgc aatggcacaa cctgccgcta ttattcgtat
    33061 aaagaacctt cgtttgcgta cgtttatcgg aattaaggaa gaagaaatta acaaccgtca
    33121 ggatattgtt atcaatgtga cgatccacta ccccgccgat aaagcgcgca ctagcgaaga
    33181 tatcaacgat gcgctgaatt atcgcaccgt aacgaaaaac attattcagc atgtagagaa
    33241 taaccgtttc tctttgctgg aaaaattaac tcaggatgtg ctcgatatcg cacgtgaaca
    33301 tcactgggtg acgtatgctg aagtggagat cgataaactg cacgcgctgc gctacgccga
    33361 ttcggtatcc atgaccttaa gctggcagcg ttaatcgcca tatcgggagg ctgcatgaac
    33421 atagtgatca ccggagggac gggattaatt ggtcgccatt tgattccgcg tttgctggag
    33481 ctgggccatc aaattacggt agtgacgcgt aacccgcaga aagccagttc cgttctcggc
    33541 cctcgggtga cactatggca agggcttgcc gatcaaagca acctcaacgg cgttgatgcg
    33601 gtaatcaacc tggccggaga accgattgct gataaacgct ggactcacaa gcaaaaagag
    33661 cgtctctgcc aaagccgctg gaatatcacg caaaaactgg tcgatttgat taatgctagc
    33721 gacacgccgc cgtcggtact catttccggc tcggcaacgg gctattatgg cgacttaggt
    33781 gaagtggtgg ttaccgaaga ggaaccgccg cataacgaat ttactcataa actctgcgcc
    33841 cgctgggaag agattgcctg ccgggcgcaa agtgacaaaa cgcgagtgtg cctgctgcga
    33901 accggcgtgg tactggcacc ggacggcggt attctcggta aaatgctgcc accgtttcgt
    33961 cttggcctgg gcgggccgat tggttccggt cggcagtatc tggcctggat tcatattgat
    34021 gatatggtca acggcattct ctggctgctg gataacgagc tgcgcgggcc atttaatatg
    34081 gtttcgccct acccggtacg caatgaacaa tttgcccatg cgctcggtca tgcgctgcat
    34141 cgcccggcta ttttgcgcgt ccccacaacc gccattcggc tgttaatggg cgaatcttca
    34201 gtactggtat taggtggaca acgcgcgctg cctaaacggc tggaagaagc gggttttgcg
    34261 tttcgctggt acgatttaga agaggcgctg gcggatgtcg ttcgctgatg tggtttacag
    34321 caaacatccg ccagttaact ccctgtgtta caggattagt ggctttgcgc gataagatcg
    34381 tctggtgaaa gtcgggtcac catcataact aactctctgt ctaaacctct atccagcatc
    34441 tcctgagcaa tacgcagggc ttcttcgtgt ttgccctgca ttgcgccttc ttcacgtaat
    34501 ctgtcagcaa tggtcatcag tttctccttt tcttgtggtg cgcgttctgt tatctcacca
    34561 ataaatgcac ggaaacgctg ggcatcccct gtttgtaata cgtaattaaa cagggctttt
    34621 agctgtctgt cattagtgtt ccctgtaact agcagcgaaa caatttggtc gactaatccc
    34681 aacagatcgc gctgacgaat atgtttctga attaactcca acagcgccat tttgcggtgt
    34741 tgcataatct cgtcatccgg caccacggta atatccacca acggaaacgc tgatgaatat
    34801 attttgcggg ctatagcagg ctcggcaaat tcatcaagcc agcagagtga ataaggataa
    34861 ggacttctgc aaccatgata aaacagcatc gggatcacca atggaagctc tttatagccc
    34921 gcatcaagat ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat
    34981 tcttccggct tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt
    35041 ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt
    35101 ggttctagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata
    35161 ttaataaaat tccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc
    35221 ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc
    35281 gacaataacc acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt
    35341 cagaagtatt tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc
    35401 cccttgaaaa tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt
    35461 aaggaactgt tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc
    35521 ttcttctatt ttcccctgat ggaggaaaat gacgtgagta gaaacatggc gagcaaagcc
    35581 catttcgtga gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg
    35641 taacacttcg cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac
    35701 ttctggttcc atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag
    35761 atgcaccgga tatttcccct gcgcacgttc gtctatcccg actttcgcca gatacttcac
    35821 cgcccgctcg cgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac
    35881 gttttccaac accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg
    35941 tgtgcgcagt aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg
    36001 caccagattg atcgtctggc cattgaccac gatcgatcct tcactcggtt tttcgaggaa
    36061 gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac
    36121 atctccggca ttcgcttgca gtgagacccc tttcagcact tcgtgttcgc catagcgttt
    36181 gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag
    36241 gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag
    36301 agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg
    36361 cgttaatatc gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcgcag
    36421 tagagtgcag catcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag
    36481 aaggcaaaat aatgcagcga tacattttaa aggtcgagaa gccataggct ctggcggctt
    36541 caatttcccc atgcggaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc
    36601 aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa
    36661 ggaattcggt tcccttaaca atctcaagcg tgtacatgcc ggaatagaac accagcaact
    36721 gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt
    36781 atttattact ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta
    36841 ccgacaaaat aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc
    36901 acagcagcgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc
    36961 cacggagtag cggcgctcaa ggaacagcag cacaccattg gaaacggtgg tgaaaaccag
    37021 gtaaatcacg ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag
    37081 ctgcgtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag
    37141 gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat
    37201 gatccgccga aacacttgcc cgcgagtaaa accgaacgcc gtcgccgcct ctatatgccc
    37261 tttcggcact gccataaacg cgccacggaa cgtttcggta aaataagcac cgtagataaa
    37321 accgagagtg ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc
    37381 ctccgtcacc atgtttagcg caatctgcaa accatagaaa atcagcagca tcaacaccaa
    37441 gtccggcacg cctcgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt
    37501 ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct
    37561 gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatacaa
    37621 catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc
    37681 gaagggagcg ggctttcttt caccgatggc gattaaccac cataaacatc aaaatcgaag
    37741 tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc
    37801 agtgcctcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac
    37861 agtttttcat ctttaacaga cggaccaccg aatttgtaat ctttaccgac gggttgtttg
    37921 aggaaacctt cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc
    37981 aggtcagaat aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc
    38041 cagtgttcat taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct
    38101 ttcagcgact cgactgtcgg ctgaatgtca gaatttttcg ccaccaccag acgagaatcg
    38161 gcagcgtaca gtttgtcggt gaaggcaatt tcttgctgac gtttttccgt aatggaaagc
    38221 gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcggg
    38281 ttttcgacaa acgtacattg cgtattgatg cgcttgcaca actccttcgc cagctcgata
    38341 tcgaaaccaa ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg
    38401 gtaccgatgc ggatgttttg cggaattgca gcaagtgccg cagttgcgct ggagaaggcc
    38461 agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt
    38521 atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa
    38581 aatatcccgc cccgttagac gtattatttt tcattaaaaa tgcttaagta cgcatttcaa
    38641 tgcaccacga tagtgcaaca aacctggcgt gcaccatgat gatcattcca tcaggtacag
    38701 cttcccagcg acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt
    38761 gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc
    38821 agcataatct ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac
    38881 tgatgagcca gcaaaagcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc
    38941 agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg aatagaccaa
    39001 atcctggttg gcataggcca ccacatccac gcctttacta cgccaggtct cgttagcgta
    39061 agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt
    39121 tggctgaatc ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc
    39181 ggagaaggca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatagcgtc
    39241 gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cccaggtaca
    39301 tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc
    39361 acctttagca tctttcgacg agaacagcgc ataggtggta tcggttccga tacgtaccgt
    39421 ctccggtagc gccgcatagc tggaagccgc tgcggagaga ccgactaata aagacagagc
    39481 gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt
    39541 gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa
    39601 cattcaacgt taaatatgtt aataagacat tgcattattg tcctgaagtt gaagatagca
    39661 ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt
    39721 gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc
    39781 gggcaaagag atcttcagga agggttattg caaactggtc aagaacacga ttaaccgtct
    39841 gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaacg ggaggcataa
    39901 tcaccgcacc gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg
    39961 tttcacgcac gcagagcacc aacgggcgac gctctttcag caccacatct gccgcacggg
    40021 tcagtaagcc atcagtatag ctatggacaa tgccggacag ggttttgatt gaacagggta
    40081 aaatcaccat ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg
    40141 catcgagcgt gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg
    40201 ataaggtctg gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc
    40261 gcagaacctg taataagcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa
    40321 tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg
    40381 cgggagttgc aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta
    40441 tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt
    40501 aacgtatcga ggaaatcgag gtagccctga tcaacatctt tggtgacgta gacgccgttg
    40561 aacaccgagc attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga
    40621 tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatcaact
    40681 tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg
    40741 aagcgaattt ccggtgccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc
    40801 atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg
    40861 tttttatcgc ggaactcggc gcggttggca ttcagtttac ggcgcacgga cttacgacgc
    40921 agctgctggc ccggcatgat aaaggtgcgg ccaacatagc ggtttttaac gaagccctgg
    40981 cggtacggtt tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttctggg
    41041 atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcgcccagt
    41101 ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg
    41161 cgggcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga
    41221 cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc
    41281 aggaaatcaa agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta
    41341 cggttctcgt caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg
    41401 aaagcaacca taccgtggcc gataatcatc gccacacagg catacgcgcc gcggattaag
    41461 cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag
    41521 ttgtccagct cgctggcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg
    41581 tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg
    41641 ttgtgggcaa gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg
    41701 ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc
    41761 tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag
    41821 cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc
    41881 gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg
    41941 cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa
    42001 cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa ttgcgggatc
    42061 agctgtgatt tgctccagtc ttcacttttc gacaccccgg taaaggagtc gagaaagaag
    42121 agaatggcgg caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga
    42181 tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca
    42241 ccaacgatca gggtagcgat aaacagtacc gcgatggcaa tcccatttcg aaccagttcg
    42301 tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa
    42361 gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgtggat caggctaacc
    42421 agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca
    42481 cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc
    42541 gtagggattt ccttcccgcg catcaataaa aatggcgctg aaaaaatatt caacgccatc
    42601 gactttttat gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat
    42661 ccgacctacg gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca
    42721 agactgtatt aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc
    42781 aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg
    42841 gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc
    42901 agcttaccga caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca
    42961 taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc
    43021 gcaggtggta cttcaacctt tggtttaggc ggctccaccg gtttcggctt cggtggggcg
    43081 accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc
    43141 gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc
    43201 ggcaacgcct gggtggcggc tggcatcata tcaggctcat cacgatcgcc cgctttcggc
    43261 accagcggga tagccgcgaa ctcatcctga taatgttttt tctgcccgtc cagcagccct
    43321 ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac
    43381 ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa
    43441 agaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc
    43501 ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt
    43561 ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca
    43621 ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc
    43681 gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc
    43741 gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc
    43801 gctggcaatc ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc
    43861 cgccagcgcc gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt
    43921 gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc
    43981 ccgacgttgt aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc
    44041 cggctcaccg acaattgccg gtttttcgct gcggaagatg cctgctttct cgagaccaat
    44101 actttcgcga tctggaccca gccagtcggt atgatccagc gcaatactgg ttactaccgc
    44161 gacatcggca tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat
    44221 caccacgtca agttgtgcct gcttgaacag ccacaacgcc gacagcgtac cgtactcgaa
    44281 ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc
    44341 cgattccggc aattcctggc cctgcacacg tacgcgctcg gtataacgca ccagatgagg
    44401 cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca
    44461 ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcaggac
    44521 gccaagacgc gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg
    44581 caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt
    44641 gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg
    44701 ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac
    44761 gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct
    44821 cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgcgc ctttctcgat
    44881 caggaattca ctgcgctgga atctaggcgg cagtttttcg cgaacggtct gttcgataac
    44941 acgcggaccg gcaaagccga ttaacgcttt aggttcagcg atgttgagat cgcccagcat
    45001 ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa
    45061 gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat
    45121 cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc
    45181 ttcctgcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc
    45241 gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt
    45301 catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagatgcca gacggtcttt
    45361 atacttcttg gagtcacgaa acttcagctc atctttcggc tcaagctcgc tacccagctc
    45421 cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg
    45481 atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg
    45541 accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt
    45601 aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt
    45661 cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa
    45721 tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga
    45781 gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacggattat ttcgatgacg
    45841 cccggcaaaa tagaaaccac aataatcccg acgatcagca gcttaaggtt atcctgaacc
    45901 atcgggattg taccgaagaa atagcctgcg taggtaaaaa gcagtaccca caacagtgcg
    45961 ccgatcacgt tataagcagc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac
    46021 ggggcgaacg ttctgacgat cggcacaaaa cgggcgagaa taatcgtttt gccgccatgt
    46081 ttctcataaa actgatgggt tttgtcgaga taactgcgac ggaaaatttt cgaattggga
    46141 ttactgaata acttttcacc gaacaaccgc ccgatagtgt aattgaccgc gtcacccaca
    46201 atcgcggcga tcagcatcag cactaccatc atatggacgt tgagatcgtt ggtttccagt
    46261 gatgccaacg ctccggcaac aaacagcaat gaatcacccg gtaaaaacgg cattaccacc
    46321 agaccggttt cacagaacaa aattaagaac aaaatggcat aaacccagac gccgtactcc
    46381 gcgactagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc
    46441 aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta
    46501 cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg
    46561 cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc
    46621 ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct
    46681 ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg
    46741 atgtacaaag gcattcgctt tgatatcaac caccacataa ggaccgtgac gcgtgacatt
    46801 aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt
    46861 gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg
    46921 ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat
    46981 gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc
    47041 tttaacccaa cgcacggcga tgtcaccagg taaattcgca tttacgccca aggtccacgc
    47101 cgcgtctttg cgctgcgcgg ttgtttcgaa atgcaccact tgcccggtac cgtgtacccc
    47161 tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc
    47221 cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt
    47281 actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga
    47341 cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg
    47401 ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca
    47461 ttacgcacgc agccaacaga aagatgcggc gtacccgaag catcccctac ctgagtcggg
    47521 aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct
    47581 tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc
    47641 ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact
    47701 tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc
    47761 atattgaacg ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc
    47821 aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa
    47881 atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc
    47941 gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg
    48001 gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac
    48061 agtccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg
    48121 gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg
    48181 gcatcctgca cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta
    48241 cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg
    48301 gcttcgccca tagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg
    48361 gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag
    48421 attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac
    48481 tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc
    48541 gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc
    48601 gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc ggaccatgca
    48661 gcgtaatgcg gccaaactca ggcgcgggca gcaacgtatc cagcgcaacg tgttgttcat
    48721 gcccaataaa cttgctatag gcttcaaaca cttgcgtcgt gccacgcgct ttaccctcca
    48781 gggtatagcc tgcgatatgc ggcgtgccga tatccacttt tgtcagcaat tccacattaa
    48841 gttccggctc gccttcccag acatccagca ccacgcttaa cttctggcct tcattcaggc
    48901 aggtcagcag cgcggtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc
    48961 cgggcttcag gctacggatc agtttttcat ccgccagatg tagcgttttg tacggaccat
    49021 ctttaaagag tggcgtatgg aaagtcagaa tatccgcgcg ctggactaac tcatccagcg
    49081 agcggaaatc accctcatcc ccacggtcgg cgcgaggcgg atcgcaaagt aaggttttaa
    49141 tccctaacgc ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg cccacgatcc
    49201 ccaccgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagc agggaggaga
    49261 aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg
    49321 cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtt ccaacaaatt
    49381 taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg
    49441 catccgcgtc tgccagttga gcgacgggga ttggacgccc gggaaccgcg atcacctcac
    49501 ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt
    49561 ttgtgttacc tgtatgagac gagagttaac cggacaagta tgccataatc tcgcggccag
    49621 gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct
    49681 cctcctgggc agccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct
    49741 ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg
    49801 caacaacaaa gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat
    49861 tcaccgctgc tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg
    49921 gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt
    49981 ctggggaatc atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg
    50041 ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag
    50101 atggttattc cgcaaccaca gcagcgcgag gcgaccgacc gtcctttatt accggcggag
    50161 cacaatgcgc taaaacagct ggtgaccaaa cttgcggcgg caacggggga acccagcaaa
    50221 cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg
    50281 aaactgttta accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat
    50341 acgccgacgc tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg
    50401 gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct
    50461 tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat
    50521 ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat
    50581 atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg
    50641 ctggttcgtt aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag
    50701 atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc
    50761 ggcccggaga cgccgagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg
    50821 cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa
    50881 catccggctc cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag
    50941 agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca
    51001 aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg
    51061 gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc
    51121 ccgataaccg caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc
    51181 ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg
    51241 ccgttacagg cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa
    51301 ccgtaatggc tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc
    51361 gccattccgt tccatgacat cactttgcca gagtgttttg gccctacgat gcctaacccc
    51421 caggtcagag cgcctgtcag taactggctt tcaccaaacc caagaatcaa acgcccgatg
    51481 accaacaggg caaatttgaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg
    51541 ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc gccatattga
    51601 tcggccagtc gcccggcgta accacgcgtc agcaccgtag ccagaaactg aatcccgacg
    51661 gcaatgccga ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc
    51721 ggcaacggca accctacggt catgtaggtg agaaaaaccg caaaagagat gcggaagagc
    51781 gaaaaactgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga
    51841 atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacggtgcg
    51901 ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc
    51961 tagaactggt ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt
    52021 tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga
    52081 cctactccgg cctactgcga attaatcttt tcagcttgcg cattaccagc gtggcgttgg
    52141 tgccgccgaa gccgaagctg ttagacataa cagtggtcag ttcgcgatcg gtcgtttcgg
    52201 tcacgatgtt cagacccgca gcctgctcgt ccagctcttc aatgttgatg ctcggggcga
    52261 taaagccgtg ttccagcatc agcagagagt agatagcttc ctgcacaccc gcagcgccca
    52321 gtgagtgacc ggtcatggct ttggttgcag aaatcgccgg gctcttatcg ccgaacactt
    52381 cacggatagc tgccagctct ttcacgtcgc caaccggagt cgaagtaccg tgagagttca
    52441 ggtaatcgat tggggtatca acgccatgca tcgccatctg catacaacgt actgcgcctt
    52501 cgccagacgg agcaaccatg tctgcaccat cagaggttgc gccgtagcca acgatttcag
    52561 catagatgta agcaccacgc gccagcgcgt gttccagctc ttcaaccact accataccgc
    52621 cgccgccagc gataacgaaa ccgtcacggt gagcgtcgta agtacgggag gctttttccg
    52681 gggtgtcgtt gtatttagta gatagcgcac ccatcgcgtc gaattcgcag gccatttccc
    52741 agcacagctc ttcgccgccg ccagcaaaca cgatgtcctg tttgcccagt tggatctgct
    52801 ctactgcgtt accgatacag tgtgcggaag tcgcacacgc ggagctgatg gagtagttaa
    52861 cgccatgaat tttaaacggc gtggcgagac aggcagaaac gccggatgcc atcgctttag
    52921 tgaccacata cgggccaacc gctttcaggc cgcgcgggcc gcgcattgcg tcagcgccga
    52981 acacctggaa acgcggggaa ccgccgccgg aacctgcaat caggccaacg cgcgggttat
    53041 tctggtaagc ttccggagag aggcccgcat cagcgattgc ctgctccata gaaaggaatg
    53101 cataaatgga tgcgtcgctc ataaagcgca caactttgcg gtcaatgagg ccagtggtat
    53161 ccagttttat gttgccccag acgtggctac gcatgccgga atccttcagc tcctgagaga
    53221 aagtgatccc tgaacgtcct tcacgcagag atgccaggac ttcctgctgg ttattaccga
    53281 tgctggaaac aatgcccagg ccagtaatca ctgcacgttt cattcaatac ctctgtaagt
    53341 cgcacataga gtaagtttcg aatgcacaat agcgtacact tgtacgccga acaagtccga
    53401 tcagccattt aatagagata tttgcgcagc cttacacaca tcgctaagat cgagccaccg
    53461 cctgtaagac gagtaactta cgtgaaacac tactccatac aacctgccaa cctcgaattt
    53521 aatgctgagg gtacacctgt ttcccgagat tttgacgatg tctatttttc caacgataac
    53581 gggctggaag agacgcgtta tgtttttctg ggaggtaacc aattagaggt acgctttcct
    53641 gagcatccac atcctctgtt tgtgatagca gagagcggct tcggcaccgg attaaacttc
    53701 ctgacgctat ggaaggcatt tgatcagttt cgcgaagcgc atccgcaagc gcaattacaa
    53761 cgcttacatt tcattagttt tgagaaattt cccctcaccc gtgcggattt agccttagcg
    53821 catcaacact ggccggaact ggctccgtgg gcagaacaac ttcaggcgca gtggccaatg
    53881 cccttgcccg gttgccatcg tttattgctc gatgaaagcc gcgtgacgct ggatttatgg
    53941 tttggcgata ttaacgaact gaccagccaa ctggacgatt cgctaaatca aaaagtagat
    54001 gcctggtttc tggacggctt tgcgccagct aaaaacccgg atatgtggac gcaaaatctg
    54061 tttaacgcca tggcaaggtt ggcgcgtcca ggcggcacgc tggcgacatt tacgtctgcc
    54121 ggttttgtcc gccgcggttt gcaggaagcc ggattcacga tgcaaaaacg taagggcttt
    54181 gggcgcaaac gggaaatgct ttgtggggtg atggaacaga cattaccact cccctgctcc
    54241 acgccgtggt ttaatcgtac gggcagcagc aaacgggaag tagcgattat cggcggtggt
    54301 attgccagcg cgttgttgtc gctggcgtta ttacggcgcg gctggcaggt agcgctttat
    54361 tgcgcggatg aagcccccgc gctgggtgct tccggcaatc gccagggggc gctgtatccg
    54421 ttattaagca aacacgatga ggcgctaaac cgctttttct ctaatgcgtt tacttttgct
    54481 cgtcggtttt acgaccaatt acccgttaaa tttgatcatg actggtgcgg cgtcacgcag
    54541 ttaggctggg atgagaaaag ccagcataaa atcgcacaga tgttgtcaat ggatttaccc
    54601 gcagaactgg ctgtagccgt tgaggcaaat gcggttgaac aaattacggg cgttgcgaca
    54661 aattgcagcg gcattactta tccgcaaggt ggttggctgt gcccagcaga actgacccgt
    54721 aatgtgctgg aactggcgca acagcagggt ttgcagattt attatcaata tcagttacag
    54781 aatttatccc gtaaggatga ctgttggttg ttgaattttg caggagatca gcaagcaaca
    54841 cacagcgtag tggtactggc gaacgggcat caaatcagcc gtttcagcca aacgtcgact
    54901 ctcccggtgt attcggttgc cgggcaggtc agccatattc cgacaacgcc ggaactggca
    54961 gagctgaagc aggtgctgtg ctatgacggt tatctcacgc cacaaaatcc ggcgaatcaa
    55021 catcattgta ttggtgccag ttatcatcgc ggcagcgaag atacggcgta cagtgaggac
    55081 gatcagcagc agaatcgcca gcggttgatt gattgtttcc cgcaggcaca gtgggcaaaa
    55141 gaggttgatg tcagtgataa agaggcgcgc tgcggtgtgc gttgtgccac ccgcgatcat
    55201 ctgccaatgg taggcaatgt tcccgattat gaggcaacac tcgtggaata tgcgtcgttg
    55261 gcggagcaaa aagatgaggc ggtaagcgcg ccggtttttg acgatctctt tatgtttgcg
    55321 gctttaggtt ctcgcggttt gtgttctgcc ccgctgtgtg ccgagattct ggcggcgcag
    55381 atgagcgacg aaccgattcc gatggatgcc agtacgctgg cggcgttaaa cccgaatcgg
    55441 ttatgggtgc ggaaattgtt gaagggtaaa gcggttaagg cggggtaatc tgctctcgca
    55501 gtgtttgccg gatgcggcgt gaacgcctta tccaacctac gtgtgacgtg taggcctgat
    55561 aagacgcgat aagcgtcgca tcaggcatgg tgctccagac gccgcaactt acagctgtga
    55621 cgcctgctga aacaggtttt cccacatatc ggtcaccagc gcctggtcac gcggcgacaa
    55681 ttcaccggca ccaatggctt tttccagact ctggctaacg gtcgtatgca ccgcctgagc
    55741 ggagtggtca tcaccacttt ccagttctgc gatggctaat gtcaggtggc cacgcaaata
    55801 cccactggca aacaactcat catcactggc atggtcaacc atgccgtcga ttaatgccag
    55861 aatgcgtgat tcaaactccg cgatcatctt ctttcctcat tgtaaaacgt ggcgctggct
    55921 tcagttgagc gcttccggcc acgggaactg ttccgccgta agttccggcg tgtgataata
    55981 attttgtagt gctttaatga agcgtgccgg gcgttccgga atgccatttg tcagataatc
    56041 catcacctgc gcatgtaccc gccgttgaaa caccacacga tccggttcga aatccccttc
    56101 cagattgtcg caactgacgt taaacggata tccagccgcc acgcagaata accagtcgaa
    56161 cgcctgcggc ttcacttcaa catcttcaaa ctggctttgc gtctgggcat cgcgtccgtc
    56221 cgggcaatac cagtagccga aatcaaccag ctcacggcgc gcttttccgg caatacacca
    56281 gtgcgaaatc tcatgaattg cgctggcata aaagccatga gcaaagacga ttcggttata
    56341 cggtacttcc gcatcagcag gaagatagat cggttcgtcg tcgcctttaa tcagacgggt
    56401 attaaattca tcggcaaagc agctattaaa aatttcaatc aactgctcgt agtggtgtgt
    56461 actgttcatt agttcatccc caaccagtgg aggatctcct gtccgtggct atcgtaaagt
    56521 aatttggcac tcatcaccgc cgagacgata acaatcatcg ggcggatcag cttttgtcct
    56581 ttgctcaaca ccaaccgtga acccatgcgc gcgccgagga actgccccac cagcatgaca
    56641 aagcccgtcg cccaaatcac tttgccgccg agaataaaca gcagcagacc gccaatgttt
    56701 gacgttgcgt tgagtaattt ggcgtgagcc gtggctttgg cgaggttgaa tccgcacagc
    56761 ataacgaagg ccagcgcgta aaacgatccg gcagccgggc caaagaatcc gtcataaaaa
    56821 ccgacgcagc caccggcaat taacgcgaag ggtaaaccgt acatccggcg ctggcgatct
    56881 tcttcaccca gctttggcat cagcaagaaa tagaggccga tacaaatcac cagaatgggc
    56941 aaaatctgcc gtaagacatc agcctgaacg tactgtacca acagtgcgcc gctcattgag
    57001 ccgacaaagg tcatggcaat attgagtttc tgatcgctta aactaaccac tttgcggcga
    57061 ataaagtaga tagtggcgga aatagagccg ccgcaggctt gcagtttatt ggttgccagc
    57121 gcgttagcgg gagacattcc cgcagccatc aatgccggaa tggtgagtaa cccaccgcca
    57181 ccggcaatcg agtcgataaa tcccgccagc atggcgacaa aaaagagaac tcccagcaac
    57241 agcggagaaa ccataaacag gctattaaac gtttccatta gatcacgtgc tcatccagta
    57301 gcgcctggca ggaaggcggc aacggaggcg gtgtcttctt ctcaggcttt gttgttcccg
    57361 gttttggagg ttcaaaccag ctttgcagtt ctgccccgca accatcgcct gatggcggta
    57421 aaggttgatc ttcacactcc agactatcgg cagggcaacg taatcgtaca tgcatatgcg
    57481 cgcgatgctg gaaccagggt cgcactttgc gcaaccagtc gcgatcggtg cccgcatcaa
    57541 ggcaaagttg ttgtttaatc gccggattaa caaaaatgcg cgtgacgtct ttgtcctggg
    57601 cggcgagttt gatcaagctg aaaatttctg gcttccacag cgtggagaca acgtgtttac
    57661 cgtcgcggga aactaagtcc agtgcttgcg ggcgcaagag ctgcgcggag gtccagcgag
    57721 ttttcggcag ttgcagaaag atatcgacat ccagtccggt ctggtggctg gcatgaccgc
    57781 cgttgaaacg cccaccagcg ggcatcccca tatcgccaat cagcaccgta cccatgccca
    57841 gattgctcac ctggctactc agacgctgga taaacatcac cagatccggg tgaccgaaat
    57901 agcgacgctg atcggtacgc atgacctgat aatgttcgga ctgtatcggc aacgtgtcag
    57961 cgccgacaat acagccatta gaaaaactgc ctatcgattg tgcgctaccc ggcacaggtt
    58021 gggttatttt ttgccacggc gtcgctgcca ggctagcact actggcaagc agagccagca
    58081 gcgcaatcgc ggttttgttc attttttacc agcgtggaat atcagtcttc acatcggcat
    58141 tttgcgcccg ctgccgtaac aggtgatcca ttaaaacgat cgccagcatc gcttcagcga
    58201 tcggcactgc acggatcccg acacagggat cgtgacggcc tttggtgatc atctcaactt
    58261 cttcgccaaa gcggttaata gtgcgccccg gcacggtaat gctggaggtc ggtttcagcg
    58321 ccatatgagc aatgatttgc tgcccgctgc tgataccgcc gaaaatgccg cccgcatggt
    58381 tgctctggaa accgtctttg gtgatttcgt cgcggttctg gctgccgcgc agcgccacta
    58441 cgtcaaaacc gtcgccaatt tccacgcctt tcaccgcgtt gatgctcatc agcgcatggg
    58501 cgatgtcggc atccaggcgg tcaaagaccg gctcgccaag tccggcgggg acaccactgg
    58561 caacaacggt gactttcgcg ccgatggagt cgccctcttt tttcagcgcg cgcatcagtt
    58621 catctaacgc gtcgattttg tcggggtccg ggcagaagaa cggattttgt tcaaccagcg
    58681 accagtcttt gatttccagc ggaatgtcgc ccatctgggt caggcagccg cggatttcaa
    58741 taccaaattt ctcggcgaga tattttttgg caatcgcccc tgccgccacg cgcatggcgg
    58801 tttcgcgggc ggaagaacgt ccaccgccgc gataatcgcg cagaccgtat ttttgttcgt
    58861 aggtgtaatc agcatggcct ggacggaaga cgtctttaat tgcgctgtaa tcctgagaac
    58921 gctggtcggt attttcgatc aacaagccaa tgctggtgcc agtagtaacg ccttcaaaaa
    58981 caccggagag tattttgacc tgatccggct cgcggcgctg ggtggtatag cgcgatgtcc
    59041 cagggcgacg acgatcaagg tcatgttgca ggtccgcttc cgtcagcgga atgcctggcg
    59101 gaacaccatc gacgatgcag ccgagcgcca gcccgtgcga ttcgccgaag gtggttacgc
    59161 gaaagagttg tccaattgtg tttccagcca tcacggctcc gttattgttg tgtttgcgtg
    59221 tttacttaat ctttataaat cgcgaaatgt tctcgtgcgg caataagctg ctctttagtg
    59281 agcataaaca caccatcgcc gccgttatca aactccagcc aggtgaacgg aacatcggga
    59341 tattgttcca taagatgtac catgctgttg cccacttcac aaatcaacac gccatcatca
    59401 gcaaggtaat ctgccgcgtt accgagaatg cgacgcgtca gtttcaggcc gtcggtgcca
    59461 gatgccaggc ccagttccgg ctcgtgacgg tattcgtttg gcaggtcgga catatcttcc
    59521 gcgtcgacat acggcgggtt agtgacaatc agatcgtatt gcactttcgg caaatcacgg
    59581 aacagatcgg aacgaatcgg aatgacgttg tggatcagac cgtgttcttc gatgttctgt
    59641 tcggcaaccg ccagcgcgtc gggagagata tctaccgcgt cgacttccgc ttccgggaag
    59701 gcataagcac aggcaatggc gatgcagccg ctaccggtac acatatctaa aatatgctgc
    59761 ggttgcttgc tgatgagtcc ggcaaattta ttgttgatca gttcaccaat cggcgagcgc
    59821 ggcaccagca cgcgttcatc gacgtaaaat tcatgaccgc agaaccaggc tttgttggtc
    59881 aggtaagcca ccggaatgcg ctcgttgacg cggcggatca cgcgttcaac aatacggtgt
    59941 ttttcgctgg aggtcagacg cgcggtgcgc atatcttccg ggatatccag cggcaggtag
    60001 agcgatggta acaccagctg tacggcttca tcccacgggt tatcggtgcc gtgaccgtac
    60061 cagatatttg ccgcgctgaa gcggcttacc gaccagcgca atatgtcctg aatggtttgc
    60121 agctcattta ctgcttcatc aacgaaaatt ttatccacgt attcctccag ggcatgatcg
    60181 caataatttc ggcggctagt ttgccatgaa gatgacgata aatcagcatt cacgcgcggt
    60241 gagtgaggaa aaatacgttt aaaacgatcg attgtgctac gagtcgggta aactgtagga
    60301 aaattagaaa tagagacaga taaatgaaaa agaaaacaac acttagcgag gaggaccagg
    60361 ctctgtttcg ccagttgatg gcggggactc gcaagattaa gcaggacacg attgtccacc
    60421 gaccgcagcg taaaaaaatc agcgaagtgc cggtgaaacg cttgatccag gagcaggctg
    60481 atgccagcca ttatttctcc gatgagtttc agccgttatt aaataccgaa ggtccggtga
    60541 aatatgttcg cccggatgtc agccattttg aggcgaagaa actgcgccgc ggcgattatt
    60601 caccggaatt gtttttggat ttacacggtc tgacgcagct gcaggccaag caggaactgg
    60661 gcgcgttgat tgccgcctgt cgccgcgaac atgtgttttg tgcctgcgtg atgcatgggc
    60721 acgggaagca tattttgaag caacaaacac cgctgtggct ggcacaacat ccgcatgtga
    60781 tggcatttca ccaggcaccg aaagagtacg gcggtgatgc ggctttgttg gtgttgattg
    60841 aagtggaaga gtggttgccg ccggagttgc cctgaatgct ggcagcaaat gccggagcag
    60901 cgtaaacgcc ttatccggcc tacaaatatg tgataattca ataaattgca attcacttcg
    60961 taggcctgat aagacgcatc agagcaatac tacgaacttc agttcattca atacatactg
    61021 aaaacgacac gtcgaatcaa tcggttgccc actactggta ttcacattca ccacaatatc
    61081 gtgtagtact ttatccgtta ccggatgacg agttggcgta accgttaccg ttgatttatc
    61141 acaaccgtaa gaggtaaagg ttaacaattc tgataccgcg cggcagtatg ctcctctgcc
    61201 atcattatca gaaaaattac tgccatcaca gagtctgccg ccttccatca ggtaggtcga
    61261 aagcgtggtg gtcactgttg accccgtgga ctgaattcgt aacgccttat tatggctgac
    61321 ataattttca ctccaggaga tatcgtatga agcgaccggt atcgttgggc atattgtgtt
    61381 atgcactgta ggggccgtat agcttccaaa tccgggtaaa tctgtatagg aataaaagga
    61441 tgcgatccaa gggcaaccat aatcatcaga tgcaacggta attttaaacg tcatatatgc
    61501 acaacgaaaa attttcgtat ctatgccagg gcaatttgag ttattaattg tatcaaagtc
    61561 agtcagtacc aaagttggag ctgaacccga cccagaccag gaaataaccc gcaacgtatc
    61621 gcctttatac gcaaaagaat catatttccc atatgcaacc tcctttggcg ttatcaccgt
    61681 aacattgaaa acactgctcg ctacagcacc ggttaccttg tccatggcat aataaactct
    61741 cacactgtca aaactcgcac tcataagttt atctccagca taactcttag atgcgccgac
    61801 aacactcaga acgagcagca ttaggctcgc gaaaattatg gtccattttt tcatttcaga
    61861 aatactcaaa ggttgccgtc actgtggcac taaagtctcc ggacgttaca tctcgcccgc
    61921 tctttgcctg taaccaggta ttaaaattta ggctgttatt tccctgattc agcgggagcg
    61981 tgacgcctgt tgtattgttg attggcaccg atgtgcctgc cgcagtttca ataccaatcc
    62041 cagcgccgga tgctgatgac gaggagtcaa aggccagaaa gccgggtaga gcactatctt
    62101 ctgttcctgt caacgttact ttgacattaa acaatgtaga actatgacaa tctttcaatt
    62161 gaaacaccaa tggcactcgc tctgactgcc caaggttcat taaatcactg gcggcaatgg
    62221 caggaaaatg tacttctgcc aactggctac cattaatcac tggcgtacac gatttactga
    62281 ttaaattgcc ggtaaagtgc aaattatctc ctgctattgt ggttccactg cccagcaaca
    62341 tcaggctact aataaaaaac aacgtacgct ttttcttcat tggtaatcta cctgtaacgt
    62401 ggcccaggcc tcaaagtccg cctctggcag aatgacacca ctctttttta ctggcacagc
    62461 cgttaacacc ggtaaatcgg tttcattcac cacaagtggc gtattgatcg taaaactctg
    62521 ccctgcctgt ttgagctgaa tgcctaaacc agtgatattg ctgcttactg cagcattatc
    62581 aaaatcgctt ggtgtgccac tccagctcaa ggtcatttcc attaatgggt cgcgaaccgc
    62641 cgtatcgcag gtaatggaat acggcacggt ttgctcataa ttgctgccat taatgtcatc
    62701 aataagcaca tcctgaaaag ttatttcaat ggtttgagcg ttgctaatcg tgcagttagg
    62761 cggctgcact aaataaccgt gaaaagagac gtttgagagt gccatcgagc aaaagcccca
    62821 cagcaaaata agcgagatcc gtttcattgg taatccacca caattgtggc actggcgtta
    62881 aattcagctg ccgctaacgt tgtaccactc tgtttcaccg gcacagcttc cagaacggga
    62941 actcccgagg aaaaattaaa tggcaaccat gagccagagg ttaagtcgag tattgtgtga
    63001 tcactacttt tctgaacccg aataccaaac ccaggaatac ccgtgctgat aactgtttca
    63061 ccgttaatca cgaccgttgt cgcctgcatc tgtagacgca ggtcatccat tgccagattg
    63121 ttacaaacaa gcgagtagtc aatgggacgt ttataatcaa ccctattgat cttattaatg
    63181 aatacattac caaactcaac tgccgcacca tttaccgtac aaggaggagg ggcatcgaca
    63241 agcacccgta aattcatact tactgactta ctcgctgcaa ctgcaaagcc gctaccaaca
    63301 caacatatca gtaacccagt taccgcccag acagaacaag atcctttcat actggctctc
    63361 ccggcctcag cctttgcggc tctgctcttc atcgacctag caactgttac cgttgcaagt
    63421 gaaaaagagc ggcatccgag cgccatagtc gttgacatag gtcagtaccg gcacgctacc
    63481 catcttcaca ttcagcggtg cagaggtttt cggcgacatc actattggcg agaaaccggc
    63541 tgccggattg ccatttttct gtgtgctggc attactgaaa atgacgtaat acgacgtcgg
    63601 gttattgacg gtaaattcct gaccgttgcg ggttaaagta atcttatatt gctaggggtt
    63661 tttcatgctg acgttctcca gcgccttcgg tcgccagaac aacttaatac gcgtttgtag
    63721 tgctatttgc agcgtgttgg ctttgtttga ttttggcggg atctcacgca cgttgaagta
    63781 gaacaggctt tcacgatcgg caggcaattt gttgatatcc ggcattccct gcacttttac
    63841 ctgcccgttc atcattgaat cgatgcgttg caccggtggc agaacagtta acggtgaact
    63901 gatcttgtta cccttttcat cttcgatcca gctttgggcc agataaggca gtttcggatc
    63961 gttattgcgc aacgtcacgc tgattgattt gtcgctttca ttaaatatca aacgggtacg
    64021 atctggcgtt acagaagcct gggcagacaa actggcagcg ctaagcagta acgctagcgt
    64081 cgtagcaccc aatttggcag agcaaagtaa atcagacatg gatcattcct taatcacgtt
    64141 attggttagc tgaaacagaa acagggggat tggtgggtgt cacctgttgc gtctgctcct
    64201 gaatgacagg ctttatgtca tcaggaacaa ccggcgcgac gttgccttta tgctggcatg
    64261 gcagtaacaa gccgttaaac agatcggcag gcagcgggtc cggcaggttg atatcgcaat
    64321 gcgcaacacc actccagaac acactcatgt gttcgccagg tttcactcca gccagataaa
    64381 cattgccgtc atcatcgaca aggcccactg tctgctcgtt atcatttttt acttctgcgc
    64441 caaacggagg atggctgccg tcttgtaaac gcagcaccgc catcgctttt tgaccactga
    64501 tgacggcaaa tttgcggtag ccaatcgccc cttcagttag cgtggcttgt accaccgact
    64561 gggttgcttc ggcgttttcc ggcagtttgt tgaggtcgat atacgcctga ttgcggtaat
    64621 agttattgac gtcagaaacg acggctttac caaacatatt ggtataaaca gcagcaccgt
    64681 taccttctac cggaacatcg gcaacgccat cggcatcaat caacaagcgt gtcccgccca
    64741 tattctgggt acggtgaagt gcgccaccgt gggcagtaag cgttgcgccg ccctgtaacg
    64801 acaagcccgc agaggtgtat tgcccttcat ggtagttcgc actgaggtca acctgcgcca
    64861 gcgaaccatc atggctgtaa tagccgtcaa cgctggtgtg tttgtcactg gtgccgacgt
    64921 tcaactgata gtgagtcgcg tcatcgacac ggctgaaata accgacctga ctgctgtccg
    64981 tcccactgcc atagtaccgt tatagctaac ggtactgttg tcgccccacg gcatactgag
    65041 cgaaatgtac atgcctttgt cggcctggtt gtcatactcg taacggtagc cagtcatcga
    65101 gatgctgaca ttacgaatac tgcccatatt gaaatagtgc gagagcatga tgttgtagtt
    65161 tgtctgctcc tcgcgatccc agtaggtatg gcgggtatag ttgagataaa ccgaaacacc
    65221 cgcatcgcgg aagttctggt tataagtggc ggtgtacatc tctttgtcgt tgcccgtgcg
    65281 taccattccg ctgtcactgg catccagata ctcgctcatg gtcataaagt tctcttccga
    65341 gaagcgatat ccagcaaaag taacgcggct gttgagctgg tcaaagtctt tggaataact
    65401 cacacggaag gagttaccgt ccagcgaacc tttgccataa gcggtgtctt tatccagttt
    65461 ggtgtgcgag tgagtaacat caaacgcgac cgcgccgaat gtagacaaat cgcgaccgac
    65521 accaagcgcc gcagactgat agttttcatc tcccagtgcg ccgccatata acgaccagcc
    65581 gttagcgatc ccccaggaag cttcagcacc agaaaaaaat tcaccctcga catggtgtcc
    65641 ccactcttgc ggacggccca tcatgatctt atagcgaacc tgacctggac gagtgaggta
    65701 tggcatcgag gcggtgctga tgtcatattc ctgcacctgg ccgttctgtt cttcaatgcg
    65761 aatatgcaac gtaccggaga cagaatcacc aagatcctga atacgaaacg gtccagccgg
    65821 aacctgcgtt tcgtaaatca cacgccccat ctggctgacg gtcacttttg ctgtggtgtg
    65881 tgccacgccg gaaatgtctg gcgcgtagcc gcgcagattg ggaggcaaca tttgatcgtc
    65941 agtactgaca ctgccaccaa catagttaaa accatcaaaa atatcggatc tgaggtaatc
    66001 ctcgcccagc gccagtttgg cttttaatga tggtaacgcc cgcctggcat agtagcgact
    66061 ccactcccat ttttttgagt ttcatcgccg ctgaattcat cgtcatcatt actgcgagta
    66121 tgttgatagt tagtctgcca gtcagcacgc atacgccacg gccctaggtt aaccccaacc
    66181 gtcccgttgc cgctgatctc gttactatca tcaccgccat tttcttcgtg ccgggtttgt
    66241 gcgttgatgc tgtagtccgc gacgatcccg gagatgccgt catcccaacg tgaaggcgga
    66301 tccctgtcgg gataagtata ttcgaggtaa gcctgtggca gtgaaatgac taatgcggac
    66361 tggcttaaat cagccttaat ttccatgcct tccagttgac cggatttcag gcatttagca
    66421 tcgtggctcc attgcagatt tttcgccacg tcttctttta aaccaaactg cgctaccagt
    66481 tccggtgtca gacaagcata gcttttgctc gcgtcatctt caccagcata ccagtaaata
    66541 tcgtactctt ccgccaatgg ctgtttattt agttgaacct gtaaattgta ttttccgggc
    66601 tcaacatatc cctggctgga aaaacgcttc atatcaattt ttgtgtcgcc ttttaattcc
    66661 agaaaacggg aatcaaactg aatgtcgtct tcagcccaga cactgctata actccctgac
    66721 attgccagcg caatgcacca cggaagaatc cgtaatcgaa aaagagaatg gtcaggcata
    66781 cacgtttcta tccgttttta ttcttgttcg gtcaaaatac atttaaatcc cccatagcag
    66841 tgtcgctatg ggggtatgcc ttacaggtaa gtaatctgga aggtggtatt tgcttcaaaa
    66901 ttacccagat ctggcgcatc agctgcgccg accagccagg cgttaaagtt cagtgtctgc
    66961 ttcgctttgc ctttgttggt agaagtgtct ttcactattt tctggtcaat acccatgccg
    67021 cttttgtaag aggtacccag agagtcaccg atcgccaggc taacattgtt aaatgccgca
    67081 ccggtatcgg tattgaaaat ggtgtaataa ttgccgctat ttgcagaaga aacggtacca
    67141 gtaaaggtag tggtcattgt ttcctgagta tcaaatacgc aatcttgcag acgaatctgg
    67201 aaacttttcg gcgtggtggt gccgttattt ttcagttttt cggcaccgat atcacccata
    67261 tcaacttcca gtttgtgatc gtccggaacg atagaacaca ctgagtcttc aatggtgccg
    67321 taaaaacgtg cagtaccgtt gttacccgca gcgattgttg ccgtagaggt gaacacgccc
    67381 atcaccattg ccgcagcaat agctgtttta acaaacttac tcattatttg tccttaaact
    67441 ataaataat
//
LOCUS       DAJTRD010000008        66992 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTRD010000008 DAJTRD010000000
VERSION     DAJTRD010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66992
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.008"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(72..1076)
                     /gene="cra"
                     /locus_tag="OO315_000597"
     CDS             complement(72..1076)
                     /gene="cra"
                     /locus_tag="OO315_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5889626.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1158..1244)
                     /locus_tag="OO315_000598"
     CDS             complement(1158..1244)
                     /locus_tag="OO315_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5889627.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1256..1747)
                     /gene="ilvN"
                     /locus_tag="OO315_000599"
     CDS             complement(1256..1747)
                     /gene="ilvN"
                     /locus_tag="OO315_000599"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5889628.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1750..3474)
                     /gene="ilvI"
                     /locus_tag="OO315_000600"
     CDS             complement(1750..3474)
                     /gene="ilvI"
                     /locus_tag="OO315_000600"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5889629.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3593..3637
                     /locus_tag="OO315_000601"
     CDS             3593..3637
                     /locus_tag="OO315_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889630.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3792..4736)
                     /gene="leuO"
                     /locus_tag="OO315_000602"
     CDS             complement(3792..4736)
                     /gene="leuO"
                     /locus_tag="OO315_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5889631.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5395..5481
                     /gene="leuL"
                     /locus_tag="OO315_000603"
     CDS             5395..5481
                     /gene="leuL"
                     /locus_tag="OO315_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5889632.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5574..7145
                     /gene="leuA"
                     /locus_tag="OO315_000604"
     CDS             5574..7145
                     /gene="leuA"
                     /locus_tag="OO315_000604"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5889633.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7145..8236
                     /gene="leuB"
                     /locus_tag="OO315_000605"
     CDS             7145..8236
                     /gene="leuB"
                     /locus_tag="OO315_000605"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5889634.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8239..9639
                     /gene="leuC"
                     /locus_tag="OO315_000606"
     CDS             8239..9639
                     /gene="leuC"
                     /locus_tag="OO315_000606"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5889635.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9650..10255
                     /gene="leuD"
                     /locus_tag="OO315_000607"
     CDS             9650..10255
                     /gene="leuD"
                     /locus_tag="OO315_000607"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5889636.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10304..11482)
                     /gene="setA"
                     /locus_tag="OO315_000608"
     CDS             complement(10304..11482)
                     /gene="setA"
                     /locus_tag="OO315_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5889637.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11584..11715)
                     /gene="sgrT"
                     /locus_tag="OO315_000609"
     CDS             complement(11584..11715)
                     /gene="sgrT"
                     /locus_tag="OO315_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5889638.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11804..13459
                     /gene="sgrR"
                     /locus_tag="OO315_000610"
     CDS             11804..13459
                     /gene="sgrR"
                     /locus_tag="OO315_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5889639.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13493..13592
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13623..14606
                     /gene="thiB"
                     /locus_tag="OO315_000611"
     CDS             13623..14606
                     /gene="thiB"
                     /locus_tag="OO315_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5889640.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14582..16192
                     /gene="thiP"
                     /locus_tag="OO315_000612"
     CDS             14582..16192
                     /gene="thiP"
                     /locus_tag="OO315_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5889641.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16176..16874
                     /gene="thiQ"
                     /locus_tag="OO315_000613"
     CDS             16176..16874
                     /gene="thiQ"
                     /locus_tag="OO315_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5889642.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16988..17752)
                     /locus_tag="OO315_000614"
     CDS             complement(16988..17752)
                     /locus_tag="OO315_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5889643.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17838..18716)
                     /gene="araC"
                     /locus_tag="OO315_000615"
     CDS             complement(17838..18716)
                     /gene="araC"
                     /locus_tag="OO315_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5889644.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19055..20755
                     /gene="araB"
                     /locus_tag="OO315_000616"
     CDS             19055..20755
                     /gene="araB"
                     /locus_tag="OO315_000616"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5889645.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20766..22268
                     /gene="araA"
                     /locus_tag="OO315_000617"
     CDS             20766..22268
                     /gene="araA"
                     /locus_tag="OO315_000617"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5889646.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22468..23163
                     /gene="araD"
                     /locus_tag="OO315_000618"
     CDS             22468..23163
                     /gene="araD"
                     /locus_tag="OO315_000618"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5889647.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23238..25589
                     /gene="polB"
                     /locus_tag="OO315_000619"
     CDS             23238..25589
                     /gene="polB"
                     /locus_tag="OO315_000619"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5889648.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25754..28660
                     /gene="rapA"
                     /locus_tag="OO315_000620"
     CDS             25754..28660
                     /gene="rapA"
                     /locus_tag="OO315_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5889649.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28672..29331
                     /gene="rluA"
                     /locus_tag="OO315_000621"
     CDS             28672..29331
                     /gene="rluA"
                     /locus_tag="OO315_000621"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5889650.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29448..30263)
                     /gene="djlA"
                     /locus_tag="OO315_000622"
     CDS             complement(29448..30263)
                     /gene="djlA"
                     /locus_tag="OO315_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5889651.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30518..32872
                     /gene="lptD"
                     /locus_tag="OO315_000623"
     CDS             30518..32872
                     /gene="lptD"
                     /locus_tag="OO315_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5889652.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32925..34211
                     /gene="surA"
                     /locus_tag="OO315_000624"
     CDS             32925..34211
                     /gene="surA"
                     /locus_tag="OO315_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5889653.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34211..35200
                     /gene="pdxA"
                     /locus_tag="OO315_000625"
     CDS             34211..35200
                     /gene="pdxA"
                     /locus_tag="OO315_000625"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5889654.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35197..36018
                     /gene="rsmA"
                     /locus_tag="OO315_000626"
     CDS             35197..36018
                     /gene="rsmA"
                     /locus_tag="OO315_000626"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5889655.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36021..36398
                     /gene="apaG"
                     /locus_tag="OO315_000627"
     CDS             36021..36398
                     /gene="apaG"
                     /locus_tag="OO315_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5889656.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36405..37247
                     /gene="apaH"
                     /locus_tag="OO315_000628"
     CDS             36405..37247
                     /gene="apaH"
                     /locus_tag="OO315_000628"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5889657.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37325..37804)
                     /gene="folA"
                     /locus_tag="OO315_000629"
     CDS             complement(37325..37804)
                     /gene="folA"
                     /locus_tag="OO315_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5889658.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37995..39857)
                     /gene="kefC"
                     /locus_tag="OO315_000630"
     CDS             complement(37995..39857)
                     /gene="kefC"
                     /locus_tag="OO315_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5889659.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREA
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39850..40380)
                     /gene="kefF"
                     /locus_tag="OO315_000631"
     CDS             complement(39850..40380)
                     /gene="kefF"
                     /locus_tag="OO315_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5889660.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40428..41822)
                     /locus_tag="OO315_000632"
     CDS             complement(40428..41822)
                     /locus_tag="OO315_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5889661.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41881..42168)
                     /gene="fixX"
                     /locus_tag="OO315_000633"
     CDS             complement(41881..42168)
                     /gene="fixX"
                     /locus_tag="OO315_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5889662.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42165..43451)
                     /locus_tag="OO315_000634"
     CDS             complement(42165..43451)
                     /locus_tag="OO315_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5889663.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43502..44443)
                     /locus_tag="OO315_000635"
                     /pseudo
     CDS             complement(43502..44443)
                     /locus_tag="OO315_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44458..45228)
                     /locus_tag="OO315_000636"
     CDS             complement(44458..45228)
                     /locus_tag="OO315_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5889664.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45584..47098
                     /gene="caiT"
                     /locus_tag="OO315_000637"
     CDS             45584..47098
                     /gene="caiT"
                     /locus_tag="OO315_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5889665.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47129..48271
                     /gene="caiA"
                     /locus_tag="OO315_000638"
     CDS             47129..48271
                     /gene="caiA"
                     /locus_tag="OO315_000638"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5889666.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48400..49617
                     /gene="caiB"
                     /locus_tag="OO315_000639"
     CDS             48400..49617
                     /gene="caiB"
                     /locus_tag="OO315_000639"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5889667.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49691..51244
                     /gene="caiC"
                     /locus_tag="OO315_000640"
     CDS             49691..51244
                     /gene="caiC"
                     /locus_tag="OO315_000640"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5889668.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51353..52138
                     /gene="caiD"
                     /locus_tag="OO315_000641"
     CDS             51353..52138
                     /gene="caiD"
                     /locus_tag="OO315_000641"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5889669.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52144..52734
                     /gene="caiE"
                     /locus_tag="OO315_000642"
     CDS             52144..52734
                     /gene="caiE"
                     /locus_tag="OO315_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5889670.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52820..53215)
                     /gene="caiF"
                     /locus_tag="OO315_000643"
     CDS             complement(52820..53215)
                     /gene="caiF"
                     /locus_tag="OO315_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5889671.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMCR"
     gene            complement(53477..56698)
                     /gene="carB"
                     /locus_tag="OO315_000644"
     CDS             complement(53477..56698)
                     /gene="carB"
                     /locus_tag="OO315_000644"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5889672.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIIEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56716..57864)
                     /gene="carA"
                     /locus_tag="OO315_000645"
     CDS             complement(56716..57864)
                     /gene="carA"
                     /locus_tag="OO315_000645"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5889673.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58320..59141)
                     /gene="dapB"
                     /locus_tag="OO315_000646"
     CDS             complement(58320..59141)
                     /gene="dapB"
                     /locus_tag="OO315_000646"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5889674.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59308..60222)
                     /gene="rihC"
                     /locus_tag="OO315_000647"
     CDS             complement(59308..60222)
                     /gene="rihC"
                     /locus_tag="OO315_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5889675.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60288..61238)
                     /gene="ispH"
                     /locus_tag="OO315_000648"
                     /gene_synonym="lytB"
     CDS             complement(60288..61238)
                     /gene="ispH"
                     /locus_tag="OO315_000648"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5889676.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61240..61689)
                     /gene="fkpB"
                     /locus_tag="OO315_000649"
     CDS             complement(61240..61689)
                     /gene="fkpB"
                     /locus_tag="OO315_000649"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5889677.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61776..62270)
                     /gene="lspA"
                     /locus_tag="OO315_000650"
     CDS             complement(61776..62270)
                     /gene="lspA"
                     /locus_tag="OO315_000650"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5889678.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62270..65086)
                     /gene="ileS"
                     /locus_tag="OO315_000651"
     CDS             complement(62270..65086)
                     /gene="ileS"
                     /locus_tag="OO315_000651"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5889679.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65129..66070)
                     /gene="ribF"
                     /locus_tag="OO315_000652"
     CDS             complement(65129..66070)
                     /gene="ribF"
                     /locus_tag="OO315_000652"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5889680.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66078..66296)
                     /locus_tag="OO315_000653"
     CDS             complement(66078..66296)
                     /locus_tag="OO315_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5889681.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66399..66662
                     /gene="rpsT"
                     /locus_tag="OO315_000654"
     CDS             66399..66662
                     /gene="rpsT"
                     /locus_tag="OO315_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5889682.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16249 a        18241 c        17246 g        15256 t
ORIGIN      
        1 taccttgatg tccagtcccg tactctacgc gccagaggga aattcacctg gcgcgtattt
       61 ttgttcgcgg attagctacg gctgagcacg ccgcggcgat agagattacg tttaatgcgc
      121 gttaaaccag gttttggctt acgcggttcg tccaggcttg ccaggacaat ctccagcaca
      181 cgctctgcga catcgcggtg acgttgagcc actgccagca ccggacactg taagaagtcg
      241 agcagttcgt tatcgccaaa ggtggcaatt gccaggtcag aaggcagttt gccgtcgcga
      301 cgcagcgtga catccatcac tccttgcaac aacgcaaacg acgttgtgaa cagcgcctgc
      361 ggcatcggat gcgtttccag ccatttttcg aataactggg cagccgcctc ccgctcatag
      421 ctgttggcat acaggaaatg cacttcgcgc ggatcatctt tccaagcagt acggaaacct
      481 tgttcacgca ggaagctgac agaaagctcc ggcagcgcgc caagataaag caccgtctcg
      541 gcgggaaact tacgtaactc ttccgccagc atttcggcat catcctgatc ggcaccaacc
      601 acgctggtga aatgttcacg atcgagggcg cggtccagcg cgacaatcgg gaacgggtcg
      661 ttagcccagc gttgataaaa aggatgctca ggtggcaacg acgtcgaaac aataatggca
      721 tcaacctgac gctgtaaaag gtgctcaatg cagcgcattt cgttgtctgg ctgatcttct
      781 gagcaggcaa tcagcagttg ataaccccgt tgccgcgcct ggcgttcaag atagttagcg
      841 atgcgggtat agctggtgtt ctccagatcg gggatcacaa gaccaataga acgtgtgcgt
      901 ccagcacgaa gcccagctgc cacggcgttc gggtggtaat tgtgctcacg caccacagcc
      961 atgacttttt caacggtttt gtcgctcaca cggtattgct tcgctttgcc gttaataaca
     1021 tagcttgcag tggtccgcga cactcccgcc agccgagcga tttcatccag tttcacaatt
     1081 gccccttgcg taaaatgtaa aaaccatagc cattgtactg gtatgggtta aattcattaa
     1141 catctaagcg cagaaaatta actgcggcaa ctgcttttat tccaggttcc cactgatttc
     1201 gcaaaaaaag cccaacgtga catgttgggc tgcaaattgc gcactgagat cattctcaac
     1261 gcattatttt atcgccgcgc gaaagtccga ccacaccaga gcgagcaact tccacaattt
     1321 tcgccacatc gcgaatcgat gctaaaaatg catcaagctt atcgctggtg cctgctaatt
     1381 gaacggtata aagcgagggg gtgacatcga taatttgccc acggaatatt tccgtattac
     1441 gtttcacttc gtcacgcccg taaccgctgg cctgaatttt caccagcatg atttcccgct
     1501 caacatgcgc gccctgcccc aactcactca cgcgcaagac atcgaccagc ttgtgtaatt
     1561 gcttttcgat ctgctcaagt actttttcat cgcccacggt ctggatggtc atacgcgata
     1621 atgtcggatc gtcggttggc gcaacggtca ggctttcaat gttgtagcct cgctgggaaa
     1681 aaaggccaat cacgcgggat aacgcgcctg attcattttc gagtaagact gataatatcc
     1741 ggcgcataat caggttctct ccgttttgct taaccacatt tcatccattc cgcccccgcg
     1801 aatctgcatc gggtagacgt gctcactgcc atcgacggta acatcaacaa acaccaggcg
     1861 attattgcgc acctgttcca gcgcctcgct aagtttgctt tccagctcat gcggatgaga
     1921 gatctggatc ccgacgtgac cataggcttc cgccagacgg acgaaatcgg gtagtgattg
     1981 catataagat tgtgaatgac ggccggaata gatcatgtcc tgccactgtt tcaccatccc
     2041 cagatagcgg ttattgagat tcaccaccag tacgggcaac tcgtattgca acgcggtaga
     2101 cagttcctgg atgttcatct gaatactgcc gtcgccagtg acgcaaacca cggtttcttc
     2161 tggcaacgcc attttgacgc ccagtgccgc aggtaaacca aaacccatcg tgccgagacc
     2221 gccggaattg atccagcgac gcggtttgtc gaatggataa taaagcgcag caaacatctg
     2281 gtgctgcccg acatcggacg tcacgtaagc gtccccatta gtcaaccgcc aaagagtctc
     2341 gatcaccgcc tgcggtttaa tcttttcact gtgagtgtca tatttcaggc actgaagagc
     2401 gcgccactgt tcaatctgct gccaccagtc gcggatctca tccagcggtt gatgggcgga
     2461 ttcttgcgac aggagttcaa gcatttgttc gaggacctgg cgagcatccc ccacaatcgg
     2521 gatatccgca gtcacggttt tagaaatgga agtaggatca atatcgatat gcagaacagt
     2581 ggcatttggg cagtactttg ccagattgtt cgtcgttcgg tcatcaaatc gtaccccgac
     2641 ggcgaaaatc acatccgcgt tatgcatcgt catattggct tcgtaggtac cgtgcattcc
     2701 cagcatgccc agtgcctgac gatgcgttgc cggaaacgcc cccattccca tcaatgagga
     2761 aacaacgggc agattcaacg cctccaccgt ttctttcaac tgctgatggc agcccgccat
     2821 gattgcccca ccgcctacgt agacaaccgg tttttttgcc gctaccagcg tttgcagagc
     2881 acgcttaatt tgccctttat gtccggtagt agtgggattg taagaacgca tactgaccga
     2941 ctcagggcag acatagggta atttgttcgc cggattaaga atatctttcg gtaaatcaac
     3001 gaccactggt cctgggcgac cacttgccgc cagccagaaa gcctttttca gcacctgcgg
     3061 aatgtcttcc gtttgcttaa ccagaaaact gtgtttaacc accggtcgcg aaatccccac
     3121 catgtcgcac tcctgaaagg catcgtaacc tatcaacgag gtcgctacct gcccggaaag
     3181 gacaactaat ggaatggaat ccatataagc ggtggcgatg ccggtaatcg cattggtcgc
     3241 ccctggaccc gacgttacca gcacgacgcc gacttccccg gtcgcacgcg ccaggccatc
     3301 ggccatatgc accgccgcct gctcatgacg aactaataca tgatcaatac cacccacggt
     3361 atgcaatgca tcgtaaatat caaggactgc gcctccggga taaccgaata cttgtttaac
     3421 gccctgatcg ataagcgatc ggacgaccat ctcggctcca gacaacatct ccatggcctg
     3481 cctcactgtt tgacggaaaa aatgtgtaaa acacattaac cgtccgatct tgcttaagca
     3541 attggcagcc agagacataa acgcagggta ataagaataa acaggaggaa aggtgaaaag
     3601 aaacgataaa atcctccatt acgaaggatt aaattgataa taaatcggct gaatcccaca
     3661 acttacagca atttctctat ttttttaatt aatcctaaaa tagagagtcc acacattcag
     3721 aataaaattc tacattcaat aaaaaatgca gaataaacca gacattcatg tctggcctat
     3781 tctgcattca gttagcgttt gcaaattgag actaattgtt cttccatcca ctgatggcct
     3841 ttatcgcgcc cggcagcttc atgccaagag agataacagg ttctgctgtt ttgttttaac
     3901 ggcagcggta atacctgtaa ttctaaggat tcagcgaact cttcagccag ccaacgcggc
     3961 gcaatagcga ccaaatgcgt ttgcgacacc acgctaagta cgctcatcat tgccatgccc
     4021 tgatacgcga tactggcttg cttatctacc gtgtcatacc aaggttgact aaatgacgcg
     4081 aaacgatcga gcgaaaccgc cgcatgttgt tcgttataaa catcatgttt cagtaacggg
     4141 cctttaattg ttggatgatt tttgctggct accagcacca tttcatcttt aaataatggc
     4201 acgctggtaa attcaggacg atggaagtct tcataactaa tcacaaactc cgtttcctga
     4261 taacgcagct gatgttcagt gttctgattt aatgaagact tgaacataac atgtatattt
     4321 ggcgcaatct gctcaatgtg attataaatc tgcgaggtca gaatgctgtc taacgggctg
     4381 caaacacaaa gatgaaatac acgttcactg ctcgcgggtt caaaacctga accaggcaat
     4441 tcattttgta ctagttgcaa tgcctggcga actgaaccaa aaagttgaaa tgcgcgagca
     4501 gtcggttgaa taccacggcc ataacgaaca aaaagctcgt cattaaacat caccttcagg
     4561 cgtgcaacag cgttactgac cgcaggttgc gacataccca gaacatgagc ggcacgggta
     4621 atgttttgct cctgcatcac ggcatcgaaa acggttaata agttgagatc gaccatgcgt
     4681 agctgtggct tgcttaactc cgccgtctct gaatgatctg tttgtacctc tggcatactt
     4741 aactccactg tcacacttaa ctccctttcc cttattggaa tgcagagaaa taatcctgaa
     4801 gaatatgatt tatcatgcat ataaaataag aaaaagcaaa atgagtaaaa ttcggaaaac
     4861 ataaagatac gggttctgat aaaaacagaa tccattgccg tggaatgagt catttaccac
     4921 agcataataa tccataatgt tttgttgcga aaacaatcta atcataacta ccgcgaatac
     4981 tcaatcatct acaaaatgga ttaaatgtgc ttatttaata attaatttat atatttaatg
     5041 cattaattct taacattaat tgttcaataa tattcaccaa atcaatatca aaaaaaatcg
     5101 caaaacatat aattcaatac aaatcatcag gataggtttt gcaacgcgtg cattttgtcc
     5161 cctttttcct cgttgattag atgcaaaaat ttatgctgaa atatgtcaac cgatgaaaag
     5221 cgtcggtagt taagcagaaa ttaatatcgc ttactttaac caccgtagca caattagcta
     5281 attttacgga tgcagaactc acgctggcgg gacgttttta ttgcgtcagg gttgacatcc
     5341 gtttttgtat ccagtaactc taaaagcata tcgcattcat ctggagctga tttaatgact
     5401 cacatcgttc gctttatcgg tctactacta ctaaacgcat cttctttgcg cggtagacga
     5461 gtgagcggca tccagcatta agccagcacg cagtcaaaca aaaaacccgc gccattgcgc
     5521 gggttttttt atgcccgaag cgaggcgctc taaaagagac aaggacccaa accatgagcc
     5581 agcaagtcat tattttcgat accacattgc gcgacggtga acaggcgtta caggcaagct
     5641 tgagtgtgaa agaaaaactg caaattgcgc tggcccttga gcgtatgggt gttgacgtga
     5701 tggaagtcgg tttccccgtc tcttcgccgg gcgattttga atcggtgcaa accatcgccc
     5761 gccaggttaa aaacagccgc gtatgtgcgt tagctcgctg cgtggaaaaa gatatcgacg
     5821 tggcggccga atccctgaaa gtcgccgaag ccttccgtat tcataccttt attgctacct
     5881 ccccaatgca catcgccacc aagctgcgca gcaccctaga cgaggtgatc gaacgcgcta
     5941 tctatatggt gaaacgcgcc cgtaattaca ccgatgatgt tgaattttct tgcgaagatg
     6001 ccgggcgtac acccattgcc gatctggcgc gagtggtcga agcggcgatt aatgccggtg
     6061 ccaccaccat caacattccg gacaccgtgg gctacaccat gccgtttgag ttcgccggaa
     6121 tcatcagcgg cctgtatgaa cgcgtaccta acatcgacaa agccattatt tccgtacata
     6181 cccacgacga tttaggcctg gcggtcggca actcactggc ggcggtacat gccggtgcac
     6241 gccaggtgga aggtgcaatg aacgggatcg gcgagcgtgc cggtaactgt tcgctggaag
     6301 aagtcatcat ggcgattaaa gttcgtaagg atattctcaa cgtccacacc gccattaatc
     6361 accaagagat atggcgcacc agccagttag ttagccagat ttgtaatatg ccgatcccgg
     6421 caaacaaagc cattgttggc agcggcgcat tcgctcactc ctccggtatc caccaggatg
     6481 gcgtgctgaa aaaccgcgaa aactacgaaa tcatgacacc agaatctatt ggtctgaacc
     6541 aaatccagct gaatctgacc tctcgttcgg ggcgtgcggc ggtgaaacat cgcatggatg
     6601 agatggggta taaagaaagt gaatataatt tagacaattt gtacgatgct ttcctgaagc
     6661 tggcggacaa aaaaggtcag gtgtttgatt acgatctgga ggcgctggcc ttcatcggta
     6721 agcagcaaga agagccggag catttccgtc tggattactt cagcgtgcag tctggctcta
     6781 acgatatcgc caccgccgcc gtcaaactgg cctgtggcga agaagtcaaa gcagaagccg
     6841 ccaacggtaa cggtccggtc gatgccgtct atcaggcaat taaccgcatc actgactata
     6901 acgtcgaact ggtgaaatac agcctgaccg ccaaaggtca cggtaaagat gcgctgggtc
     6961 aggtggatat tgtcaccaac tacaacggtc gccgcttcca cggcgtcggc ctggccaccg
     7021 atattgtcga gtcctccgcc aaagccatgg tgcacgtact gaacaatatc tggcgtgccg
     7081 cagaagtcga aaaagagttg caacgcaaaa ctcaacacaa cgaaaacaac aaggaaaccg
     7141 tgtgatgtcg aagaattacc atattgccgt attgccggga gacggtattg gtccggaagt
     7201 gatgacccag gcgctgaaag tgctgaatgc cgtgcgcaac cgctttgcga tgcgcatcac
     7261 caccagccat tacgatgtag gcggcgcagc cattgataac cacgggcaac cactgccgcc
     7321 tgcgacggtt gaaggttgtg agcaagccga tgccgtgctg tttggctcgg taggcggccc
     7381 gaagtgggaa catttaccac cagaccagca accagaacgc ggcgcgctgc tgccgctgcg
     7441 taagcacttc aaattattca gcaacctgcg cccggcaaaa ctgtatcagg ggctggaagc
     7501 attctgtccg ctgcgtgcag acattgccgc taacggcttc gacatccagt gcgtgcgcga
     7561 actgaccggc ggcatctatt tcggtcagcc aaaaggccgc gaaggtagcg gacaatatga
     7621 aaaagcgttt gataccgagg tgtatcaccg ttttgagatc gagcgtattg cccgcatcgc
     7681 gtttgaatct gctcgtaaac gtcgccacaa agtcacctca atcgacaaag ctaacgtgct
     7741 gcaatcctct attttatggc gtgaaatcgt caacgagatc gccacggaat acccggatgt
     7801 cgaactggcg catatgtaca tcgacaacgc caccatgcag ctgattaaag atccatcaca
     7861 gtttgacgtt ctgctgtgct ccaacctgtt tggcgacatt ctgtctgacg agtgcgcaat
     7921 gatcactggc tcgatgggca tgttgccttc cgccagcctg aacgagcaag gttttggact
     7981 gtatgaaccg gcgggcggct cggcaccaga tatcgcaggc aaaaacatcg ccaacccgat
     8041 tgcacaaatc ctgtcgctgg cactgctgct gcgttacagc ctggatgccg atgatgcggc
     8101 ttccgccatt gaacgcgcca ttaaccgtgc attagaagaa ggcattcgca ccggggattt
     8161 agcccgtggc gctgccgccg ttagtaccga tgaaatgggc gatatcattg cccgctatgt
     8221 ggcagaaggg gtgtaatcat ggctaaaacg ttatacgaaa aattattcga cgctcacgta
     8281 gtgtacgaag ccgaaaacga aaccccgctg ttatatatcg accgccacct ggtgcatgaa
     8341 gtgacctcac cgcaggcgtt cgatggtctg cgcgcccacg gtcgcccggt acgtcagccg
     8401 ggcaaaacct tcgccactat ggatcacaac gtctctaccc agaccaaaga cattaatgcc
     8461 tgtggtgaaa tggcgcgcat ccagatgcag gagctgatta aaaactgcaa agaatttggc
     8521 gtcgagctgt atgacctgaa tcacccgtat caggggatcg tccacgtaat ggggccggaa
     8581 cagggcgtca ccttgccggg gatgaccatt gtctgcggcg actcgcatac cgccacccac
     8641 ggcgcgtttg gcgcactggc ctttggtatc ggcacttccg aagttgaaca cgtactggca
     8701 acacaaaccc tgaaacaggg ccgtgcgaag accatgaaaa ttgaagtcca gggcaaagcc
     8761 gcgccgggca ttaccgcaaa agatatcgtg ctggcaatta tcggtaaaac tggcagcgca
     8821 ggcggcaccg ggcatgtggt ggagttttgc ggcgaagcaa ttcgtgattt aagcatggaa
     8881 ggtcgtatga ccctgtgcaa tatggcgatc gaaatgggcg caaaagccgc tctggttgct
     8941 cctgacgaaa ccacctttaa ctatgtcaaa ggccgtctgc atgcgccgaa aggcaaagat
     9001 ttcgacgacg ccgttgccta ctggaaaacc ctgcaaaccg acgaaggcgc aactttcgat
     9061 accgttgtca ctctgcaagc agaagaaatt tcaccgcagg tcacctgggg caccaatccc
     9121 ggccaggtga tttctgtgaa cgacaatatt cccgatccgg cttcgtttgc cgatccggtt
     9181 gaacgtgcgt cggcagaaaa agcgctggcc tatatggggc tgaaaccggg tattccgctg
     9241 accgaagtgg ctatcgacaa agtgtttatc ggttcctgta ccaactctcg cattgaagat
     9301 ttacgcgcgg cagcggagat cgccaaaggg cgaaaagtcg cgccaggcgt gcaggcactg
     9361 gtggttcccg gctctggccc ggtaaaagcc caggcggaag cggaaggtct ggataaaata
     9421 tttattgaag ccggttttga atggcgcttg cctggctgct caatgtgtct ggcgatgaac
     9481 aacgaccgtc tgaatccggg cgaacgttgt gcatccacca gcaaccgtaa ctttgaaggc
     9541 cgccaggggc gcggcgggcg cacgcatctg gtcagcccgg caatggctgc cgctgctgct
     9601 gtgaccggac atttcgccga cattcgcaac attaaataag gagcacacca tggcagagaa
     9661 atttatcaaa cacacaggcc tggtggttcc gctggatgcc gccaatgtcg ataccgatgc
     9721 aatcatcccg aaacagtttt tgcagaaagt gacccgtacg ggttttggcg cgcatctgtt
     9781 taacgactgg cgttttctcg atgaaaaagg ccaacagcca aacccggact tcgtgctgaa
     9841 cttcccgcag tatcagggcg cttccatttt gctggcacga gaaaacttcg gctgtggctc
     9901 ctcgcgtgag cacgcgccct gggcattgac cgactacggt tttaaagtgg tgattgcgcc
     9961 gagttttgct gacatcttct acggcaatag ctttaacaac cagctgctgc cggtgaaatt
    10021 aagcgatgca gaagtggacg aactgtttgc gctggtgaaa gccaatccgg ggatccattt
    10081 cgacgtggat ctggaagcgc aagaggtgaa agcgggagag aaaacctatc gctttaccat
    10141 cgatgccttc cgccgccact gcatgatgaa tggtctggac agcatcggcc tgaccctgca
    10201 gcacgacgac gctattgcgt cttatgaagc aaaacaacct gcgtttatga attagacccc
    10261 ttgcccggtc aaatgaccgg gctttccgct atcgtccacg tcatcaaacg tctttaacct
    10321 ttgcggttaa aaataatgcg acaacagaaa taatcgcaat tacccagtag acagcaaagt
    10381 gcccccaact ttgtgcaatt gctccctgaa taacaccagc cagaattacc ccggtagaga
    10441 tagtgttggt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa tcctgaaacc
    10501 atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt tgcagcgtca
    10561 tcaacgccat acggctatga aagaaaatca atccagtgta aaacagcact cctgccacca
    10621 ctgcaatgac catcattcgc cgcttaccat aacgtttgac atagtagcca gccagaatca
    10681 ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt ttgtctggca
    10741 atcctaaatc gctactgatc cacaatggca tatcaataat gtacatagtg ttgcaggtcc
    10801 acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag tcaccttgca
    10861 ttgataaagc attttccgac ggcagttcta cccgcgccac agacagaagc ataaatgcaa
    10921 tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcact gtaaagccgt
    10981 aattcaacgc cagcataaag gccagcggtg gaccgattac ccatgccaga gaaagctgcg
    11041 cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgcg tattcccgcg
    11101 ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc acaccacagg
    11161 tgataagcgt cagataatga cgattaaatg caaacagtag cgcattgcca atagccatca
    11221 aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt tttgccaacc
    11281 agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg atccagaaag
    11341 gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt agcgccccgg
    11401 ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc atacggcgag
    11461 ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata aaaaaaacca
    11521 gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca tgatgcaggc
    11581 aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca tggttaatcg
    11641 ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa aatagtgctg
    11701 ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg acttaatata
    11761 gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat ctgctcgtct
    11821 gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg acacaacgct
    11881 caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc tgctcaacac
    11941 catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta aacgctcgcg
    12001 tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag acctgctgga
    12061 gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg tgcggcaaat
    12121 gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc gcgtgctcta
    12181 ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg aaacccatat
    12241 tgcccggcaa atcttcagtt cgctaacgcg cataaatgag gaaaatgggg aactggaagc
    12301 agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct ttttgcgtcc
    12361 aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg cctctttaaa
    12421 acgaatcaat acgctgccgc tctattcgca tattacggac attgtgtcgc cgacgccctg
    12481 gacgctggat atccatctca cgcagccgga ccgctggttg ccgttactgc tggggcaagt
    12541 tccggcgatg atcctgccgt gcgaatggga aaccctcagt aactttgcca gccatcctat
    12601 tggcaccggt ccgtatgcgg tgattcgcaa cagcaccaat caactgaaaa ttcaggcatt
    12661 cgatgacttc ttcggttacc gggcattaat cgacgaagtt aacgtctggg ttctgccgga
    12721 aattgccgac gagccagccg gtggtctgat gctaaaaggt ccacagggcg agaaaaaaga
    12781 gattgaaagc cgcctggagg aaggttgcta ctatttactg ttcgacagcc gcacccatcg
    12841 cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa ctaatctggt
    12901 ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac tgctcccccg
    12961 ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa gcctcaccct
    13021 gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc agcagattct
    13081 ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt ggcatgaagg
    13141 agagatcgag agcgatatct ggcttaatag cgccaacttt acgctgccgc tggatttttc
    13201 gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga tcgactggca
    13261 agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt gccagcaact
    13321 ggtcgccagt aaagcaatgg tgccgcttat ccaccactgg ctgatcattc agggacaacg
    13381 cagtatgcgc ggcctgcgta tgaataccct cggctggttc gattttaaat cagcgtggtt
    13441 tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa cgccgttctc
    13501 aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg atccggataa
    13561 cgccggcgaa gggatttgag gctccttctc aagtcctttg ccactctttt ttgaggtgca
    13621 aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt ttcgctaaac
    13681 ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct ggtccaaaga
    13741 tcaaaaaagc ttttgaagcc aactgtaact gcgaactgaa actggtggcg ctggaagatg
    13801 gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc gatgtggtgc
    13861 tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt gccaaaagcg
    13921 gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact ttcgtcccgt
    13981 ttgattacgg ctatttcgcc ttcgtctatg acaagaacaa actgaaaaac ccgccgcaaa
    14041 gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag gatccgcgta
    14101 ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc gatgacgccc
    14161 cacaagcctg gcaaaaactg gcgaagaaaa ccgtcacggt caccaaaggc tggagcgaag
    14221 cctacggcct gtttttaaaa ggtgaaagcg atctggtact gagttacacc acctctccgg
    14281 cttatcacat tctcgaagag aaaaaagata actacgccgc cgcgaacttt agcgaaggtc
    14341 actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg gagctggcgc
    14401 aaaaattcct ccagtttatg gtttctccgg cttttcagaa tgcgatccca accggtaact
    14461 ggatgtatcc ggtggcaaat gtcacgctgc ctgccgggtt tgaacaattg accaaaccgg
    14521 ccaccacgct ggagttcaca ccagctgaag tggcggcaca acgtcaggca tggattagcg
    14581 aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg taagcgccgc
    14641 cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga acgcgccgca
    14701 gggtaactgg gtggcagtct ggcaggacag ctacctgtgg catgtggtgc gcttctcctt
    14761 ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc tcgcccgcgc
    14821 cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg caatgacctt
    14881 gatcctcccg gtgctagttg ctgttttcgg cattcttagc gtctatggtc gtcagggctg
    14941 gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct acggcctgca
    15001 aggcattttg ctggcgcacg tattttttaa tctgccgatg gcgagccgct tattactcca
    15061 ggcactggaa aacattcccg gcgaacaacg tcagcttgcc gcccagcttg ggatgcgcgg
    15121 ctggcatttt ttccgcttcg tcgaatggcc gtggctacgg cgacaaatcc cgccggttgc
    15181 cgcgcttatc tttatgctct gtttcgccag cttcgccacc gtgctatcgc tgggcggcgg
    15241 tccgcaggca accactatcg agctggctat ttatcaggcg ctgagttacg actacgatcc
    15301 tgcccgcgcg gcgatgctgg cgctgctcca gatggtgtgt tgccttgggc tggtgctgtt
    15361 gagtcagcga ctaagtaagg ccattgcgcc cggcaccacg ctgctgcaag gctggcgcga
    15421 tccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc tggcgctgct
    15481 gctgttgctg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc agttgccgga
    15541 agtgctggca caaccggtgc tgtggcaggc gctgtggacc tcgttgcgta ttgcgctggc
    15601 ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc gcgaactgcg
    15661 ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt tgatcctcgc
    15721 catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca ctatcggcct
    15781 gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga tcccttatgc
    15841 gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagta tgttgtgtca
    15901 gtcgctgggc attgaaggct ggtcgcgctt aaaagtggtc gagctgcgcg ccctgaaacg
    15961 tccactggcg caggcgctgg cttttgcctg cgtgctgtcg attggtgatt ttggcgtagt
    16021 ggcgttgttc ggtaacgatg atttccgtac cctgccgttt tatctctacc agcaaattgg
    16081 ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac tctgttttct
    16141 gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact gatatcacct
    16201 ggctttatca ccatttgccg atgcgtttta gcttaacggt agaacgcggc gagcaggtgg
    16261 caatcctcgg gccaagcggc gcgggtaaaa gtaccctgct gaatttgatt gccggttttc
    16321 tgacgccagc cagcggttcg ctgactatcg atggtgttga tcacaccacg acgccgccgt
    16381 cacgccgtcc ggtgtcgatg ctgtttcagg agaacaacct gttcagccat ctgacggtcg
    16441 cgcagaacat cgggctgggg ctaaatccgg ggttgaaact gaacgcggca cagcaggaga
    16501 aaatgcacgc gattgcccgt cagatgggga ttgataattt aatggcgcgg ttaccgggcg
    16561 agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggtg cgcgaacagc
    16621 cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgttacgt caggagatgc
    16681 tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gctattgatg gtgtcgcaca
    16741 gtgtggaaga tgcggcgcgg atcgccacac gctcggtggt ggtcgccgac gggcgcatcg
    16801 cctggcaggg taagactgaa gaattgttga gcggtaaggc gagtgcttcg gcaatattgg
    16861 ggattacggg ttagtatgca ctgtcagatg cggcgtgaac accttatccg acctacggac
    16921 agtgcgtttt gtaggcctga taagacgcgg tcagcgtcgc atgaggcaac ccgcacaaga
    16981 caatccccta aaccccaacc actttacgca aaatatcgat atacaccggc atcaacgggt
    17041 ggcgaattaa caccaccagc gccaccacgc cgatggcaga aatcaacggc gtcagccaca
    17101 acaaacggcc gcgcgacaaa taatgactta aacgatcggt tgctttaccg ctgcgccata
    17161 accgccagca cagccagcca ccaacccaca aaaataccgc cgtcgccagc agcaaccatt
    17221 taaactcgcc gctctgcatt ccggctggaa tatcgatcgc cgcgcccgcc agaatcccag
    17281 gcaggaagta aaacggtggc cacagcaggc agccgataat attcggcgta atgaattttg
    17341 ccaccggcag atccagcatt cccgccacca ttggcaccag cggacgcgtc gggccaacaa
    17401 agcgaccgac cagaatggtg aacatgctgt gttgatgcaa cgcatgttca gttttatcga
    17461 gtaatgcttt gtttttcttc agaaatgacc agcgatgcaa cggcttttta aaacgccagc
    17521 ccagccagaa ggagatccag tcgcccagca gacaaccgac aatccctgcc agccaagcgt
    17581 gccagaaact taactcgccg ctgccaatca gcgctcccag ccccgccatc agcaccgtac
    17641 cgggtagaat caaaccgacc agcgccagcg actccagaaa ggccaccaac accaccgcca
    17701 tcaatgaata cacggtagat tgggtaataa agtgttccag caatgcttgc ataatgtgcc
    17761 tgtcaaatgg acgaagcagg gattctgcaa accctatgct actccgtcaa gccgtcaatt
    17821 gtctgattcg ttaccaatta tgacaacttg acggctacat cattcacttt ttcttcacaa
    17881 ccggcacgga actcgctcgg gctggccccg gtgcattttt taaatacccg cgagaaatag
    17941 agttgatcgt caaaaccaac attgcgaccg acggtggcga taggcatccg ggtggtgctc
    18001 aaaagcagct tcgcctggct gatacgttgg tcctcgcgcc agcttaagac gctaatccct
    18061 aactgctggc ggaaaagatg tgacagacgc gacggcgaca agcaaacatg ctgtgcgacg
    18121 ctggcgatat caaaattgct gtctgccagg tgatcgctga tgtactgaca agcctcgcgt
    18181 acacgattat ccatcggtgg atggagcgac tcgttaatcg cttccatgcg ccgcagtaac
    18241 aattgctcaa gcagattcat cgccagcagc tccgaatagc gcccttcccc ttgcccggcg
    18301 ttaatgattt gcccaaacag gtcgctgaaa tacggctggt gcgcttcatc cgggcgaaag
    18361 aaacccgtat tggcaaatat tgacggccag ttaagccatt catgccagta ggcgcgcgga
    18421 cgaaagtaaa cccactggtg ataccattcg cgagcctccg gatgacaacc gtagtgatga
    18481 atctctcctg gcgggaacag caaaatatca cccggtcggc agacaaattc tcgtccctga
    18541 tttttcacca ccccctgacc gcgaatggtg agattgagaa tataaccttt cattcccagc
    18601 ggtcggtcga taaaaaaatc gagataaccg ttggcctcaa tcggcgttaa acccgccacc
    18661 agatgggcat taaacgagta tcccggcagc aggggatcat tttgcgcttc agccatactt
    18721 ttcatactcc cgccattcag agaagaaacc aattgtccat attgcatcag acattgccgt
    18781 cactgcgtct tttactggct cttctcgcta acccaaccgg taaccccgct tattaaaagc
    18841 attctgtaac aaagcaggac caaagccatg acaaaaacgc gtaacaaaag tgtctataat
    18901 cacggcagaa aagtccacat tgattatttg cacggcgtca cactttgcta tgccatagca
    18961 tttttatcca taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt
    19021 tctccatacc cgtttttttg gatggagtga aacgatggcg attgcaattg gcctcgattt
    19081 tggcagtgat tctgtgcgag ctttggcggt ggactgcgcc agcggtgaag agatcgccac
    19141 cagcgtagag tggtatcccc gttggcaaaa agggcaattt tgtgatgccc cgaataacca
    19201 gttccgtcat catccgcgtg actacattga gtcaatggaa gcggcactga aaaccgtgct
    19261 tgcagagctt agcgtcgaac agcgcgcagc tgtggtcggg attggcgttg acagtaccgg
    19321 ctcgacgccc gcaccgattg atgccgacgg taacgtgctg gcgctgcgcc cggagtttgc
    19381 cgaaaacccg aacgcgatgt tcgtattgtg gaaagaccac actgcggttg aagaagcgga
    19441 agagattacc cgtttgtgcc acgcgccggg caacgttgac tactcccgct atattggcgg
    19501 tatttattcc agcgaatggt tctgggcaaa aatcctgcat gtgactcgcc aggacagcgc
    19561 cgtggcgcaa tctgccgcat cgtggattga gctgtgcgac tgggtgccag ctctgctttc
    19621 cggtaccacc cgcccgcagg atattcgtcg cggacgttgc agcgccgggc ataaatctct
    19681 gtggcacgaa agctggggcg gcttgccgcc agccagtttc tttgatgagc tggacccgat
    19741 cctcaatcgc catttgcctt ccccgctgtt cactgacacc tggactgccg atattccggt
    19801 gggcacctta tgcccggaat gggcgcagcg tctcggcctg cctgaaagcg tggtgatttc
    19861 cggcggcgcg tttgactgcc atatgggcgc agttggcgca ggcgcacagc ctaacgcact
    19921 ggtaaaagtt atcggtactt ccacctgcga cattctgatt gccgacaaac agagcgttgg
    19981 cgagcgggca gttaaaggta tttgcggtca ggttgatggc agcgtggtgc ctggatttat
    20041 cggtctggaa gcaggccaat cggcgtttgg tgatatctac gcctggtttg gtcgcgtact
    20101 cggctggccg ctggaacagc ttgccgccca gcatccggaa ctgaaagcgc aaatcaacgc
    20161 cagccagaaa caactgcttc cggcgctgac cgaagcatgg gccaaaaatc cgtctctgga
    20221 tcacctgccg gtggtgctcg actggtttaa cggtcgtcgc acgccaaacg ctaaccaacg
    20281 cctgaaaggg gtgattaccg atcttaacct cgctaccgac actccgctgc tgttcggcgg
    20341 tttgattgct gccaccgcct ttggcgcacg cgcaatcatg gagtgcttta ccgatcaggg
    20401 gatcgccgtc aataacgtga tggcgctggg cggcatcgcg cggaaaaacc aggtcattat
    20461 gcaggcctgc tgcgacgtgc tgaatcgccc gctgcaaatt gttgcctctg accagtgctg
    20521 tgcgctcggt gcggcgattt ttgctgccgt cgccgcgaaa gtgcacgcag acatcccatc
    20581 agcccagcaa aaaatggcca gtgcggtaga gaaaaccctg caaccgcgca gcgaacaggc
    20641 acaacgcttt gaacagcttt atcgccgcta tcagcaatgg gcgatgagcg ccgaacaaca
    20701 ctatcttcca acttccgccc cggcacaggc tgcccaggcc gttgcgactc tataaggaca
    20761 cgataatggc gatttttgat aattatgaag tgtggtttgt cattggcagc cagcatctgt
    20821 atggcccgga aaccctgcgt caggtcaccc aacatgccga gcacgttgtt aatgcgctga
    20881 atacggaagc gaaactgccc tacaaactgg tgttgaaacc gctgggcacc acgccggatg
    20941 aaatcaccgc tatttgccgc gacgcgaatt acgacgatcg ttgcgctggt ctggtggtgt
    21001 ggctgcacac cttctccccg gccaaaatgt ggatcaacgg cctgaccatg ctcaacaaac
    21061 cgttgctgca attccacacc cagttcaacg cggcgctgcc gtgggacagt atcgatatgg
    21121 actttatgaa cctgaaccag actgcacatg gcggtcgcga gttcggcttc attggcgcgc
    21181 gtatgcgtca gcaacatgcc gtcgttaccg gtcactggca ggataaacaa gcccatgagc
    21241 gtatcggctc ctggatgcgt caggcggtct ctaaacagga tacccgtcat ctgaaagtct
    21301 gccgatttgg cgataacatg cgtgaagtgg cggtcaccga tggcgataaa gttgccgcac
    21361 agatcaagtt cggtttctcc gtcaatacct gggcggttgg ctatctggtg caggtggtga
    21421 actccatcag cgacggcgat gttaacgcgc tggtcgatga gtacgaaagc tgctacacca
    21481 tgacgcctgc cacacaaatc cacggcgaaa aacgacagaa cgtgctggaa gcggcgcgta
    21541 ttgagctggg gatgaagcgt ttcctggaac aaggtggctt ccacgcgttc accaccacct
    21601 ttgaagattt gcacggtctg aaacagcttc caggtctgcc cgtacagcgt ctgatgcagc
    21661 agggttacgg ctttgcgggc gaaggcgact ggaaaaccgc cgccctgctt cgcatcatga
    21721 aggtgatgtc aaccggtctg cagggcggca cctcctttat ggaggactac acctatcact
    21781 tcgagaaagg taatgacctg gtgctcggct cccatatgct ggaagtctgc ccgtcgattg
    21841 ccgtagaaga gaaaccgatc ctcgacgttc agcatctcgg tattggtggt aaggacgatc
    21901 ctgcccgact gatcttcaat acccaaaccg gtccagcgat tgtcgccagc ctgattgatc
    21961 tcggcgatcg ttaccgtctg ctggttaact gtatcgacac ggtgaaaaca ccgcactccc
    22021 tgccgaaact gccggtggcg aatgcactgt ggaaagcgca accggatctg ccaactgctt
    22081 ccgaagcgtg gatcctcgct ggtggcgcgc accataccgt cttcagccat gcgctgaacc
    22141 tcaacgatat gcgccagttc gccgagatgc acgacattga aatcacggtg attgataacg
    22201 acacccgcct gccagcgttt aaagacgcac tgcgctggaa cgaagtgtat tacggatttc
    22261 gtcgctaagt agccacatca ggtatgtaac gcctgatgcg acgctgacgc gtcttatctg
    22321 gcctacacgc tgcgattttg taggccggat aagcaaagcg catccggcat tcaacgcctg
    22381 atgcgacgct ggcgcgtctt atcaggccta cgcgctgcga ttttgtagac cggataagcg
    22441 aagcgcatcc ggcacgaagg agtcaacatg ttaaaagatc tcaaacgcca ggtattagaa
    22501 gccaacctgg cgctgccaaa acacaacctg gtcacgctca catggggcaa cgtcagcgcc
    22561 gttgatcgcg agcgcggcgt ctttgtgatc aaaccttccg gcgtcgatta cagcgtcatg
    22621 accgctgacg atatggtcgt ggttagcatc gaaaccggtg aagtggttga aggtacgaaa
    22681 aagccctcct ccgacacgcc aactcaccgg ctgctctatc aggcattccc ctccattggc
    22741 ggcattgtgc atacgcactc gcgccacgcc accatctggg cgcaggcggg tcagtcgatt
    22801 ccagcaaccg gcaccaccca cgccgactat ttctacggca ccattccctg cacccgcaaa
    22861 atgaccgacg cagaaatcaa cggcgaatat gagtgggaaa ccggtaacgt catcgtagaa
    22921 acctttgaaa aacagggtat cgatgcagcg caaatgcccg gcgttctggt ccattcccac
    22981 ggcccgtttg catggggcaa aaatgccgaa gatgcggtgc ataacgccat cgtgctggaa
    23041 gaggtcgctt atatggggat attctgccgt cagttagcgc cgcagttacc ggatatgcag
    23101 caaacgctgc tggataaaca ctatctgcgt aagcatggcg cgaaggcata ttacgggcag
    23161 taatgactgt ataaaaccac agccaatcaa acgaaaccag gctatactca agcctggttt
    23221 tttgatggat tttcagcgtg gcgcaggcag gttttatctt aacccgacac tggcgggaca
    23281 ccccgcaagg gacagaagtc tccttctggc tggcgacgga caacgggccg ttgcaggtta
    23341 cgcttgcacc gcaagagtcc gtggcgttta ttcacgccga tcaggttccc cgcgctcagc
    23401 atattttgca gggtgaacaa ggctttcgtc tgacaccgct ggcgttaaag gattttcacc
    23461 gccagccggt gtatggcctt tactgtcgcg cccatcgcca attgatgaat tacgaaaagc
    23521 gtctgcgtga aggtggcgtt accgtctacg aggccgatgt gcgcccgcca gaacgctatc
    23581 tgatggagcg gtttatcacc tcaccggtgt gggtcgaggg tgatatgcac aatggcacta
    23641 tcgttaatgc ccgtctgaaa ccgcatcccg actatcgtcc gccgctcaag tgggtttcta
    23701 tagatattga aaccacccgc cacggtgagc tgtactgcat cggcctggaa ggctgcgggc
    23761 agcgcatcgt ttatatgctg gggccggaga atggcgacgc ctcctcgctt gatttcgaac
    23821 tggaatacgt cgccagccgc ccgctgctgc tggaaaaact caacgcctgg tttgcaaacc
    23881 acgatcccga tgtgatcatc ggctggaacg tggtgcagtt cgatctgcga atgctgcaaa
    23941 aacatgccga gcgttaccgt cttccgctgc gtcttgggcg cgataatagc gagctggagt
    24001 ggcgcgagca cggctttaaa aacggcgtct tttttgccca ggctaaaggt cggctaatta
    24061 tcgacggtat cgaggcgctg aaatccgcgt tctggaattt ctcttcattc tcgctggaaa
    24121 ctgtcgctca ggagctatta ggcgaaggaa aatctatcga taacccgtgg gatcgaatgg
    24181 acgaaattga ccgccgtttc gccgaagata aacctgcgct ggcaacttat aacctgaaag
    24241 attgcgagct ggtgacgcag atcttccaca aaactgaaat catgccattt ttactcgaac
    24301 gagcaacggt gaacggcctg ccggtggatc ggcacggcgg ttcggtggca gcgtttggtc
    24361 atctctattt tccgcgaatg catcgcgctg gttatgtcgc gcctaatctc ggcgaagtgc
    24421 cgccgcacgc cagccctggc ggctacgtga tggattcacg gccagggctt tatgattcag
    24481 tgctggtgct ggactataaa agcctgtacc cgtcgatcat ccgcaccttt ctgattgatc
    24541 ccgtcgggct ggttgaaggc atggcgcaac ctgatccaga gcacagtacc gaaggttttc
    24601 tcgatgcctg gttctcacgg gagaaacatt gcctgccgga gattgtgact aacatctggc
    24661 acgggcgcga taaagccaaa cgtcagggta ataagccgct ttcgcaggcg ctgaagatca
    24721 ttatgaatgc cttttatggc gtgctcggca ccaccgcctg ccgcttcttc gatccgcgcc
    24781 tggcatcgtc gatcaccatg cgtggtcatc agatcatgcg gcagaccaaa gcattgattg
    24841 aagcgcaggg ctacgacatt atctacggag ataccgactc gacttttgtc tggctaaaag
    24901 gtgcacattc ggaagaagag gcggcgaaaa tcggtcgtgc actggtgcag cacgttaacg
    24961 cctggtgggc ggaaacgctg caaaaacaac ggctgaccag cgcattagaa ctggagtatg
    25021 aaactcattt ctgccgtttt cttatgccaa ccattcgcgg agctgatacc ggcagtaaaa
    25081 agcgctacgc cgggctgatt caggagggcg acaagcagcg gatggtgttt aaagggctgg
    25141 aaaccgtgcg caccgactgg acgccgctgg cccagcagtt tcagcaggag ctgtacctgc
    25201 gtatcttccg caacgagcca tatcaggagt acgtccgcca aaccatcgac aaactgatgg
    25261 cgggtgaact ggatgcgcgg ctggtttacc gtaaacgcct tcgccgtccg ctgagcgaat
    25321 atcagcgtaa tgttccgcct catgtacgcg ccgctcgcct tgccgatgaa gaaaatcaaa
    25381 agcgcggtcg ccccttgcaa tatcagaatc gcggcaccat taagtacgta tggaccacca
    25441 acggcccgga gccgctggac taccaacgtt caccactgga ttacgaacac tatctgaccc
    25501 gccagctaca acccgtggcg gagggaatac tcccttttat tgaggataat tttgctacac
    25561 ttatgaccgg gcaacttggg ctattttgag caaaaaaaag agttcgccag ataccatttt
    25621 gatgcgtgac gaatgctttg ccatccagta ccatagcgcc ctttccattc ctggacctga
    25681 ataacaccac tacctcataa gcacggtagc gggtggttat tgcctgcaat taaagatata
    25741 gagccgaaca catatgcctt ttacacttgg tcaacgctgg atcagcgata cagaaagcga
    25801 attgggactt ggaaccgttg tcgcggtgga tgcgcgaact gtcactttac ttttcccatc
    25861 tactggtgaa aaccgtctgt acgcacgcag tgattccccc gtgacccgcg tgatgttcaa
    25921 ccctggtgat accattacca gccatgacgg ctggcagatg caagtcgaag aagtaaaaga
    25981 agaaaatggc ttgctgacct atatcggtac tcgcctggat actgaagagt ccggcgtagc
    26041 cctgcgtgaa gttttccttg atagcaaact ggtgttcagc aaaccgcagg accgtctgtt
    26101 tgccgggcag attgaccgta tggaccgctt tgcgctgcgt tatcgcgcgc gtaagtattc
    26161 cagcgaacag ttccgtatgc cgtacagcgg cctgcgcggt cagcgtacca gcctgatccc
    26221 gcatcagctc aacatcgctc atgatgttgg tcgccgccac gcgccgcgcg tcctgctggc
    26281 tgacgaagtg ggtttaggga aaaccattga agccgggatg atcctgcatc agcaactgct
    26341 ctctggcgct gctgaacgtg tgctgattat cgtcccggaa accttacagc atcagtggct
    26401 ggtagaaatg ctgcgccgtt tcaacctgcg ctttgctctg tttgatgatg agcgttatgc
    26461 cgaagctcag cacgatgctt acaacccgtt tgacaccgaa cagctggtga tttgctcgct
    26521 ggattttgcc cgtcgtagca aacagcgctt ggaacatctc tgtgaagccg aatgggacct
    26581 gctggtggtc gatgaagcgc atcacctggt gtggagcgaa gatgcgccaa gccgtgaata
    26641 tcaggccatt gaacaactgg cagagcacgt gccgggcgtt ctgctgctga ccgcgacccc
    26701 ggaacagctg gggatggaaa gccacttcgc ccgtctgcgt ctgctggacc cgaaccgttt
    26761 ccacgatttt gcgcagttcg ttgaagagca gaaaaattat cgtccggttg cggacgccgt
    26821 tgccatgctg ctggcaggta acaaactgag caatgacgaa ctgaacatgc tcggcgagat
    26881 gatcggcgag caggatatcg agccgctgtt gcaggcagca aacagcgaca gcgaagatgc
    26941 ccagagcgcc cgtcaggagc tggtttcgat gctgatggat cgccacggca ccagccgcgt
    27001 gctgttccgt aacacgcgta acggtgtgaa aggattcccg aaacgcgagc tgcacaccat
    27061 taagctgccg ctaccgacgc agtatcagac ggctattaaa gtctccggca ttatgggcgc
    27121 acgtaaaagt gcggaagatc gtgctcgcga tatgctctac ccggagcgta tttatcagga
    27181 atttgaaggt gataacgcca cctggtggaa cttcgatccg cgcgttgagt ggctgatggg
    27241 ctacctgacc agccatcgct ctcagaaagt gctggtgatc tgcgccaaag ctgccactgc
    27301 gctgcaactg gagcaggtac tgcgcgaacg tgaaggtatt cgcgctgcgg tgttccacga
    27361 aggtatgtcg attatcgaac gtgaccgcgc tgccgcctgg tttgccgaag aagacaccgg
    27421 cgcacaggta ctgctgtgct cagaaatcgg ttctgaagga cgtaactttc agttcgccag
    27481 tcacatggtg atgtttgacc tgccattcaa cccggatcta ctggagcagc gtattggtcg
    27541 tctggatcgt atcggccagg cgcacgatat tcagatccat gtgccttatc tggagaaaac
    27601 cgctcagtcg gtgctggtgc gctggtatca cgaaggtctg gatgcatttg agcacacctg
    27661 cccgaccgga cgcactattt acgatagcgt atacaacgat ctgattaact atctggcttc
    27721 accggatcaa accgaaggct ttgacgatct gatcaaaagc tgccgcgagc aacatgaagc
    27781 gctgaaagca cagctggaac agggtcgtga ccgcctgctg gaaatccact ccaacggtgg
    27841 cgaaaaagcc caggcactgg cagaaagcat tgaagagcag gatgacgata ccaacctgat
    27901 cgccttcgcc atgaacctgt tcgatattat cggtatcaat caggacgatc gcggcgacaa
    27961 catgatcgtg ctgacgccgt ccgatcatat gctggtgccg gacttccctg gcctgtcgga
    28021 agatggcatc accatcacct ttgatcgtga agtggcgctg gcgcgtgaag atgcacagtt
    28081 tattacctgg gagcatccgc tgatccgcaa cggtctggat ctgatccttt ctggcgatac
    28141 cggtagcagc acgatttcac tgttaaaaaa caaagcgttg ccggtaggta cgctgttggt
    28201 ggaactgatt tatgtggttg aagcccaggc tccgaagcag ttgcagctca accgcttcct
    28261 gccaccgacg ccggtacgta tgctgctgga taaaaacggc aacaacctgg cggcgcaggt
    28321 agagtttgaa acctttaacc gccagcttaa cgcggttaac cgtcacaccg gcagcaaact
    28381 ggttaacgcc gtgcagcagg atgttcacgc tatccttcaa ctgggtgaag cgcagatcga
    28441 gaaatctgcc cgtgcattga ttgatgcagc gcgtaacgaa gccgacgaaa aactgtctgc
    28501 cgagctgtct cgtctggaag ctctgcgtgc agtgaacccg aacattcgtg acgacgaact
    28561 gaccgccatt gagagcaacc gtcagcaggt aatggaaagc ctggatcagg caggttggcg
    28621 tctggatgcc ctgcgtttga tcgttgtaac gcatcagtaa cggagccgaa aatggggatg
    28681 gaaaactaca atccaccgca ggaaccctgg ttggttatcc tgtatcagga tgaccatatt
    28741 atggtggtca acaagccgag cggtttgttg tcagtgccgg gtcgtctgga agagcacaaa
    28801 gacagcgtga tgacgcgcat tcagcgtgat tatccgcagg cagaatcggt gcatcgtctg
    28861 gatatggcta ccagcggcgt gattgtagtg gcgctgacca aagccgcgga gcgggagtta
    28921 aaacgccagt tccgcgagcg cgagccgaaa aagcagtatg tggcccgcgt ctgggggcat
    28981 ccatcccccg cagaaggtct ggtggatctg ccgctgattt gcgactggcc aaaccgcccg
    29041 aaacagaaag tctgttacga aacgggtaaa cctgcgcaga cggaatatga agtggtggag
    29101 tatgcggcgg ataacacggc aagagtggtg ttaaaaccga ttaccgggcg ttcgcatcaa
    29161 ttgcgtgtgc atatgctggc gctggggcat ccgattctcg gcgatcgttt ttatgcatca
    29221 ccagaagcga gagcgatggc accgcgtttg ttgctacatg cagagatgtt gacgattacc
    29281 catccggcgt atggcaatag tatgacgttt aaagcgccag cggattttta atcgtcacgc
    29341 tggcgcagtt cggtaggcgg ataaggcgtt cacgccgcat ccggcaattg tgcgccgatt
    29401 gcctgatgcg acgcgggccg cgtcttatca ggcctacagt ttaaaactta tttaaaccct
    29461 ttctgctgct ttatcagctc atatgcctgc tgaatttcct gcgctttctg cttcgccatc
    29521 tccatcatct caggcggcaa acctttcgcc accagcttat cgggatggtg ttcactcatc
    29581 agcttacggt aggcacgttt gatggtggtc gcatcatccg tcggcttcac gcccagcaca
    29641 ttacaggcat cttccagcgt cgggccacgc tgcgcttgct gccagttacc accgccagat
    29701 tgctgctgat aaccgccgcc aaactgtgca ccgccctgca tcatgcgcaa aaactggtca
    29761 aactgggcgc gggagatccc taattcttca gcaatgacat acagcaccgc ccgttcattc
    29821 gggtgcagtg aaccatcagc aaacgccgcc tgaatctgga tctccagaaa catacgaatt
    29881 aagtcaaaac gaccaaagca gacactgcga aactggcgca tcttttcgcg cagcgggtaa
    29941 ttatctgatt ttcccacccg gaacgcgttt tgcgccgcag tacgggaagc gccatgaagg
    30001 ttcattcggt ccatcaactg gctggcgata tgaatatcag cctccgtgac gcgacctttg
    30061 gatttggtta aatgccccat cacttcaaaa gtggtggcaa aaaacagcgc ctgacgctca
    30121 cgctggttgg cgaaccacgc cattttacgg ctacgggctt tatcaaacat atggccaatt
    30181 aacaggccta acactacgcc ccaaaagccg ccgcccatca gtaaggccac ggccacgcca
    30241 atgatttttc cccaatactg catatattcc ccaaatcgac acacggatat cagggctatc
    30301 tcccacaata taaaggtgct tttaccgttt tccggctgcg gtcaattgtg ggacatcgcc
    30361 tataatttgc attatcatac ctgtcattca atgccgtgcc taacaccact gacgctattc
    30421 ggacaggatt aacactagcg tagagatgac gagtacgtta gtctctgacc gtttgtcacg
    30481 cgcaacgtta ccgatgatgg aacaataaaa tcaacgtatg aaaaaacgta tccccactct
    30541 cctggccacc atgattgcca ccgcccttta tagtcaacag ggactggcag ccgacctcgc
    30601 ctcacagtgc atgttgggcg tgccaagcta tgaccgtcct ctggtacagg gcgataccaa
    30661 tgacttaccc gtgactatca atgctgacca cgcgaaaggg gactacccgg atgacgccgt
    30721 gtttactggc agcgtggata tcatgcaggg taacagccgt ctgcaggccg acgaagtgca
    30781 gctccatcaa aaagaggcac caggacaacc ggagccggta cgtaccgttg atgcgctcgg
    30841 taatgtccat tacgacgata accaggtgat cctcaaaggg ccgaaaggct gggcgaatct
    30901 gaacaccaaa gataccaacg tctgggaagg tgattaccag atggtgggtc gccagggtcg
    30961 cggtaaagcg gacctgatga aacaacgtgg cgaaaaccgc tataccattc tggataacgg
    31021 tagctttacc tcctgtctgc cgggttctga cacctggagc gtggtaggta gcgaaattat
    31081 tcatgaccgc gaagaacaag ttgctgagat ctggaacgcc cgctttaagg tgggtccggt
    31141 accgatcttt tatagcccct atttgcagtt gccggtgggt gacaaacgtc gctctggttt
    31201 cttgatcccg aacgccaagt acaccaccac caactacttt gagttctacc tgccatatta
    31261 ctggaacatc gcgccaaata tggatgccac catcacgccg cattatatgc atcgtcgtgg
    31321 caacatcatg tgggagaacg aattccgcta cctctcccag gcgggcgctg gcttgatgga
    31381 actggactat ctgccttcag ataaagtcta tgaagatgaa cacccgaacg atgacagttc
    31441 acgtcgttgg ttattctact ggaaccactc cggggtcatg gatcaggtgt ggcgtttcaa
    31501 cgtcgactac accaaggtca gcgatcctag ctacttcaat gatttcgata acaagtacgg
    31561 ttccagtact gacggctacg caacgcaaaa attcagcgtt ggctatgcgg tgcaaaactt
    31621 caatgccacc gtttcaacca agcagttcca ggttttcagc gaacagaaca ccagtagcta
    31681 ctcggcagag ccgcagttag acgttaatta ctaccagaat gatgttggtc cgtttgatac
    31741 gcgtatttac ggccaggcag tgcactttgt taacaccaga gacgacatgc ctgaagcaac
    31801 ccgtgttcac ctggaaccga ccatcaattt gccgctctct aataactggg gcagcatcaa
    31861 taccgaagcg aagtttctgg caacccatta tcagcaaacc aatcttgact ggtataactc
    31921 cagaaacacg accaagctgg acgaatccgt taaccgcgta atgccgcaat tcaaagttga
    31981 cggcaaaatg gtctttgaac gcgatatgga aatgctggct ccgggttata cccaaacgct
    32041 ggaaccgcgc gcgcagtatt tgtacgtgcc gtatcgcgat cagagcgaca tctataacta
    32101 cgactcgtct ctgctgcaat ctgactactc tggcctgttc cgggaccgga cttacggcgg
    32161 tcttgaccgt attgcctccg ctaaccaagt gacgaccggt gtcacatctc gcatatatga
    32221 tgatgctgcc gttgaacgtt ttaatatttc cgttggtcaa atctactatt tcacggagtc
    32281 tcgcactggc gatgacaaca taacatggga gaatgacgac aaaacgggct cactggtgtg
    32341 ggcaggcgac acctactggc gtatctccga gcgttgggga ttgcgtggcg ggattcagta
    32401 cgatacacgt ctggataacg tagcgaccag taactccagc attgaatacc gtcgggatga
    32461 agaccgtctg gtacagctga attaccatta cgccagcccg gaatatattc aggctacgct
    32521 gcctaagtac tattccactg ctgagcaata taagaatggt atttcgcagg taggtgctgt
    32581 cgccagcagg ccaattgccg atcgttggtc cattgttggg gcctactact acgacaccaa
    32641 tgcgaacaag caagccgact ctatgttagg tgtgcaatac agctcctgct gctatgcaat
    32701 tcgcgtcggt tacgagcgga agctgaacgg ttgggataac gataaacaac atgcggtata
    32761 tgacaacgca atcggcttta acatcgaact tcgcggcctg agctccaact acggtctggg
    32821 tacgcaagag atgctgcgtt cgaacattct gccgtatcaa aacactttgt gatttgttga
    32881 tttaccacgt aatccgcagt gcggttaatt gaaatggaaa aagtatgaag aactggaaaa
    32941 cgctgcttct cggtatcgcc atgatcgcga ataccagttt cgctgccccc caggtagtcg
    33001 ataaagtcgc agccgtcgtc aataacggcg tcgtgctgga aagcgacgtt gatggattaa
    33061 tgcagtcggt aaaactgaac gctgcgcagg caaggcagca gcttcctgat gacgcgacgc
    33121 tgcgccacca aatcatggaa cgtttgatca tggatcaaat catcctgcag atggggcaga
    33181 aaatgggagt gaaaatctcc gatgagcagc tggatcaggc gattgctaac atcgcgaaac
    33241 agaacaacat gacgctggat cagatgcgca gccgtctggc ttacgatggt ctgaactaca
    33301 acacttatcg taaccagatc cgcaaagaga tgattatctc tgaagtgcgt aacaacgagg
    33361 tgcgtcgtcg catcaccatc ctgccacagg aagtcgaatc cctggcgcag caggtgggta
    33421 accaaaacga cgccagcact gagctgaacc tgagccacat cctgatcccg ctgccggaaa
    33481 acccgacctc tgatcaggtg aacgaagcgg aaagccaggc gcgcgccatt gtcgatcagg
    33541 cgcgtaacgg cgctgatttc ggtaagctgg cgattgctca ttctgccgac cagcaggcgc
    33601 tgaacggcgg ccagatgggg tggggccgta ttcaggagtt gccggggatc ttcgcccagg
    33661 cattaagcac cgcgaagaaa ggcgacattg ttggcccgat tcgttccggc gttggcttcc
    33721 atattctgaa agttaacgac ctgcgcggcg aaagcaaaaa tatctcggtg accgaagttc
    33781 atgctcgcca tattctgctg aaaccgtcgc cgatcatgac tgacgaacag gcccgcgtga
    33841 aactggagca aattgctgct gatatcaaga gtggtaaaac gacttttgct gccgcagcga
    33901 aagagttctc gcaggatcca ggttctgcta accagggcgg tgatctcggc tgggctacac
    33961 cagatatttt cgatccagcc ttccgtgacg ccctgacccg cctgaacaaa ggtcaaatga
    34021 gtgcaccggt tcactcttca ttcggctggc atttaatcga actgctggat acccgtaatg
    34081 tcgataaaac cgacgctgcg cagaaagatc gtgcataccg catgctgatg aaccgtaagt
    34141 tctcggaaga agcagcaagc tggatgcagg aacaacgtgc cagcgcctac gttaaaatcc
    34201 tgagcaacta atggttaaaa cccaacgtgt tgtgatcact cccggcgagc ccgccgggat
    34261 tggcccggac ttagttgtcc agcttgcaca gcgtgagtgg ccggtcgaac tggttgtttg
    34321 tgccgatgcc actctcctta ccgaccgggc agcgatgctc ggtttgccgc tcaccctccg
    34381 cccttattcc cccaactccc ctgcacaacc gcaaactacg ggcacattaa cgctacttcc
    34441 tgtcgcgcta cgtgaatctg tcactgcggg gcagttagcg attgaaaatg ggcattacgt
    34501 ggtggagacg ctggcgcgag cgtgtgatgg ctgtctgaac ggtgaatttg ctgcgctgat
    34561 cacaggcccc gtgcataaag gcgtcattaa cgacgcaagc attccgttta ccggtcatac
    34621 cgagtttttc gaagagcgtt cgcaggcgaa aaaagtggtg atgatgctgg cgaccgaaga
    34681 acttcgcgtg gcgctggcaa cgacgcattt accgctgcgc gatatcgcag atgctatcac
    34741 ccctgcactt ttgcacgaag tgattgctat tttgcatcac gatttgcgta caaaatttgg
    34801 tattgccgaa ccgcgcattc tggtctgcgg gctgaatccg cacgcgggcg aaggcggtca
    34861 tatgggtacg gaagagatag acaccattat tccggtgctc gacgagctgc gggcgcaggg
    34921 gatgaaactc aacgggccgc tgcctgccga taccctgttt cagccgaaat atctcgataa
    34981 cgccgacgcc gtgctggcga tgtaccacga tcagggtctt cccgtgctaa aataccaggg
    35041 cttcgggcgc ggtgtgaaca ttacgctggg cctgcccttt attcgcacat cagtggacca
    35101 cggcaccgcg cttgaactgg cgggacgtgg cgaagccgat gtcggcagtt ttattacggc
    35161 gcttaatctc gccatcaaaa tgattgttaa cacccaatga ataatcgagt ccaccagggc
    35221 cacttagccc gtaaacgctt cgggcaaaac tttctcaacg atcagttcgt gatcgacagc
    35281 attgtttctg ccattaaccc gcaaaagggc caggcaatgg tcgaaatcgg ccccggtctg
    35341 gcggcattga ccgagccggt cggcgaacgt ctggaccagc tgacggtcat cgaacttgac
    35401 cgcgatctgg cggcacgtct gcaaacgcat ccattcttag gcccgaaact gacgatttat
    35461 cagcaggatg cgatgacctt taactttggc gaactggccg agaaaatggg tcagccgctg
    35521 cgtgttttcg gcaacctgcc ttataacatc tccacgccgt tgatgttcca tctgtttagc
    35581 tatactgatg ccattgccga catgcacttt atgttgcaaa aagaggtggt gaatcgtctg
    35641 gttgcaggac cgaacagcaa agcgtatggt cgattaagcg tcatggcgca atactattgc
    35701 aacgtgatcc cggtactgga agttccgccg tcagccttta caccaccacc caaagtggat
    35761 tccgccgtcg tgcgcctggt ccctcatgca acgatgcctc acccggttaa agatattcgc
    35821 gtgttgagcc gcatcaccac cgaagccttt aaccagcgtc gtaaaaccat tcgtaacagc
    35881 ctcggcaacc tgtttagcgt cgaggtgtta accggaatgg ggatcgaccc ggcgatgcga
    35941 gcggaaaata tctctgtcgc gcaatattgc cagatggcga actatctggc ggagaacgcg
    36001 cctttgcagg agagttaacg atgatcaatt cgccccgagt gtgtattcag gttcaaagcg
    36061 tctacattga ggcacaatct tcacctgata atgaacgtta cgtttttgct tataccgtaa
    36121 ccatacgcaa tctggggcga gcgccagtgc agttgttggg gcgttactgg ctgatcacca
    36181 atggcaatgg ccgtgaaacc gaagtccagg gcgaaggagt ggttggcgtc cagccactta
    36241 tcgcgcctgg cgaagagtac cagtacacca gcggtgcaat cattgaaacc ccgctgggca
    36301 ccatgcaggg tcactacgaa atgatcgatg aaaatggcgt ccctttcagc atcgacattc
    36361 ccgtattccg actcgccgtt cccacactca ttcattaaaa gaatatggcg acatacctta
    36421 ttggcgacgt tcatggttgt tacgatgaac tgatcgcatt gctgcataaa gtagaattta
    36481 cccccgggaa agataccctc tggctgacgg gcgatctggt cgcgcgcggc ccgggttcgc
    36541 tggatgttct gcgctatgtg aaatccttag gcgacagcgt acgtctggtg ctgggcaatc
    36601 acgatctgca tctgctggcg gtatttgccg ggatcagccg caataaaccg aaagatcgcc
    36661 tgacaccgct gctggaagcg ccggatgccg acgagctgct taactggctg cgtcgccagc
    36721 ctctgctgca aatcgacgaa gagaaaaagc tggtgatggc ccacgcaggg atcacgccgc
    36781 agtgggatct gcagaccgcc aaagagtgcg cgcgcgatgt agaagcggtg ctgtcgagtg
    36841 actcctatcc cttctttctt gacgctatgt acggcgatat gccaaataac tggtcaccgg
    36901 aattgcgggg gctgggaaga ctgcgtttta tcaccaacgc ctttacccgt atgcgttttt
    36961 gcttcccgaa cggtcaactg gatatgtaca gcaaagaatc gccggaagaa gcccctgccc
    37021 cactgaaacc gtggtttgcg attcctggcc ctgtcgctga agaatacagc atcgcctttg
    37081 gtcactgggc atcgctggaa ggcaaaggta cgccggaagg tatttacgcg ctggataccg
    37141 gctgctgctg gggtgggtca ttaacctgcc tgcgctggga agataaacag tattttgtcc
    37201 agccgtcgaa ccggcataag gatttgggcg aagcggcggc gtcttaaaca cagcctgata
    37261 caggaaggcc ggataagacg cgaccggcgt cgcatccggc actagccgta aattctatac
    37321 aaacttaccg ccgctccaga atctcaaagc aatagctgtg agagttctgc gcatcggcgt
    37381 cgtggaattc gctgaatacc gattcccagt catccggctc gtaatccggg aaatgggtgt
    37441 cgccttccac ttctgcgtcg atatgcgtca gatacagttt ttgcgctttt ggcaggaact
    37501 gctcataaac gcgaccgccg ccaatcacca tgatttctgg tacgtcacca cacgccgcaa
    37561 tggcttcatc caccgatttc acccacgtta cgcgatcgtc cgtacccggt tgactgctga
    37621 ggataatatt tttgcgtcct ggcaacggac gaccgattga ttcccaagta tggcggccca
    37681 taatcacggg tttatttaag gtgttgcgtt taaaccaggc gagatcggca ggcaggttcc
    37741 acggcatggc gttttccatg ccgataacgc gatctaccgc taacgccgca atcagactga
    37801 tcattgagat ttcccgataa aaaaattgtc gccactatac gtaaagcgta aaccgtcgtc
    37861 gactggtgcg aggatgatgt tgaggaaaat tttatattct gctggcgagt ccacgctctc
    37921 cccctggact cgccgcatta caatgaaaca aaaacaaaca gttagctgta aagtgtgatt
    37981 tacgtcactc tttattaaga tgagggtttc gtttccggtt catccgccat gttgccggta
    38041 tgtttacctt cttctgttcc ctgccagcca tgtcgttgaa ttaatgacag atgttcgcgg
    38101 tcctcggtga tgatctcact taacatcgcg ctggtgcgtt tatagaccgc cgcgcgggct
    38161 ttggtgtcat tctcaaccat tgccatctct tccaccatct gaatattaaa gcggcggaac
    38221 acatcggcac gttctcgcgc ttcatacggc cccagaccta aactttccag cgccagacgc
    38281 ccggttttca gcgcaccttc aaaggtttca cgctccggct tttcaacgcc tgcctgacgc
    38341 aaacgaatgt agtgatcgac atcgcgggcg cgggcaataa tctgcaaatg cgggaaatgt
    38401 tctttcacca tctccgtcag ttgcaggttg gtttgcggat cgtcaatagc gttaatcagc
    38461 acttccgctt tcgccgctcc ggcagattcc agtaaatcca tccgcgtggc atcgccataa
    38521 aacactttca taccaaattt acgcagcgtt tcgatatggt ccggatcgtg atcgagcacc
    38581 accattttca ccccgctgga gagcagtaaa cgtccggtaa tctgtccaaa acgaccgaat
    38641 ccggcgataa tcacgcgcgg ctgttcttca tcgatctcat cggcttcacg cgcctcctcc
    38701 gtagaagatt gctcaaggcg attgaggatc accagcagaa tcggcgttgc tgccatcgac
    38761 agcgccaccg ccagggtcag cgatttcgcc cactccggct ccagcacatt cgccatctgc
    38821 gccgcaccaa ataccacaaa ggcaaactca ctgccctgcc ctaacaacac cgcaaaccag
    38881 cgacgctgtt tatttggcac ctgcaacggt cgggcaatca gccacagcat ggcgattttg
    38941 atgatgagga aaccgagcag caaaatgaca atgcgcaatg ggttttcaat cagcgtgcca
    39001 aagtctatcg acatgccaac accgatgaaa aacagcccca acagcaaacc tttaaacggt
    39061 tcgatatcgc tttccaacgc atgacggtat tccgagcttg ccagcagtac gcccgccaga
    39121 aacgcgccca tcgccatcga caagccgacc tcttccagca gcaaaccaaa gccaaacaca
    39181 aggaataacg ccacggcact aaacgcttcc cgcaagccag agcgggcaac aaaacgcagc
    39241 gccggacgcg taacatagcg ccccagcaat accaccagta ccagcgcacc cgccactttt
    39301 aacgccgaga gagcaaatgc gcccatcgtc gtcgaggcac tgctcgccgc cagtagcggg
    39361 atcatcgcca ccagcgggat tgccgcgata tcctggaaca gcagcactgc aaaggcactg
    39421 cggcccattt gcgtcaccat cagattgcgt tcattcatcg cctgcatggc aatcgccgtt
    39481 gatgagagcg ccagcgtcat accgatcaat tccgcgacct gccagcgcag cccaagtaac
    39541 atacagaaca gccccagcag gccaccgcaa atcaccatct gcaatgcgcc accgccgaac
    39601 actgccgcac gcagcttcca cagcctttgt ggatcgagtt cgaggccgat aataaacagc
    39661 atcagcacca ccccaatctc ggcaaagtgc agaatagatt cggcatcggt caccagtcgc
    39721 agcccccacg ggccaataat gcagccggcg atcaggtagc caagtaccga tcccagacca
    39781 agacgtaccg caatgggtac aatcagcgcc gccgatccga ggtagatcag cgcctgaacc
    39841 agcgtatgac tatccatgat gggcctcctg ccattccagc agacgttgct tatagtggcg
    39901 cgcctgccct tcgagggttt cgtcgtcaca aataaaggtg cagtgcatgg caaacggtgg
    39961 cagccagttc agcccgcagt agattgccgt cgcctgtagc ggctgcgaca gcacatcaaa
    40021 gcccggatgc gcaccaattt caaaatggct ttccccgccg ccggtcgtca ccgcccacag
    40081 caaatgtttg ccatgcagcg ccgtgccgcc gtgaccgtaa gcccagccgt gcgacaaaac
    40141 tttatcgatc caaagtttaa gaagcggagg aatgctgtac cactgcatcg ggtgctgcca
    40201 gacgatcaga tcggcgcgag agagcgcctc ctgctcggcg gcaatatcga tattgaagtc
    40261 aggatagagt tgataaagag agcgaatttc gacgccttcc agcgtccttg cctgttcaag
    40321 catccgttta ttcgcatggg aatgatgcgg atacggatgc gcataaatga tgaggatcat
    40381 agttattagc ccgcgtgttt acgcctttgt tttcccacaa agtgtagtca tatattcacg
    40441 cggcttatag tcaataatat tgattcagaa aagagagaaa tctgaacaaa taatcccatt
    40501 ctctgcccgc ggatcgtcat attgctggtc tgcgccagtg acattcctcg agtctccggg
    40561 gcaaacgcta cggaaatcaa caagccaaac agcgagatac ccgcccccat tagcatcgtg
    40621 ttactgatac cgtaattatt gataaatatc ggcagtgccc aggtcgaaac aatggtgcca
    40681 atacgactta aggacataat cacgcccacg gcagaggcgc ggatatccgt cgggaagagt
    40741 tcattaggat agagccactg caaattaccc ggcccgccag agaaaaaggc atacaccgca
    40801 aaggccatca ctaccagcca gatccccata tccgggatta gccccaaaac cgccaacgcc
    40861 agcgtcatca tggcaaagct gccaatcaac aatggacgcc gtccggcagt gtttagccac
    40921 agcatcggcg gaatacagcc gagcataaag aacaggctaa tcaccacatt ccctagtgcc
    40981 gcgtttttgc caacccccaa tcccaacgaa ccaacgattt gcgggccaaa ggtgtaaatg
    41041 gcgaacattg ggatcacctg gcaggtccag atggcggcaa caaacagaac aaaaggaaaa
    41101 tggcggcgat taaacagatc gcgaaaacgg gtttgctgcg gctgctcttc atcgaaagcc
    41161 accggttcgc caaacagttt tatcatcatc tcttcgcact cttttactcg ccctttgcgt
    41221 aataaccagc ggggagattc tggcagttcg aatcgaccaa tcaaaatcaa caaacaaggg
    41281 atcgccgcgc tacccagcat ccagcgccaa ccaccttcca catcataaag ccagtagccg
    41341 accagatcgg cgcaggtcgc gccgacatac cacatcgcgg caataaagct gatggaaaac
    41401 gcccgctgac gggtactgga gaactcggtg atcatcgaag tggcgatggg ataatctgca
    41461 ccgatgacaa tgccgatgag tacccgcatc accaacagtt cgacggggga tgaaacacac
    41521 atcgtcgcca ccgatatcac gccgatggcg atgatatcaa tgaggaacat tttgcgccgt
    41581 ccgactttat cggagatata gccaaacagc gatgtgccaa cgaacagccc ggcgagcgtt
    41641 cccgcgccca gcaagccaat ccagtcagcg tccagtttca gcgccggagt cagttgctcc
    41701 agcgccacgc caatcattac cagtacataa ccatccagaa acggaccacc gcttccccac
    41761 agcaaaatgc ggcggtgaat agaggagaat ttgagatcgt caaagtttct ggacggttgc
    41821 atgggttatt cctgtttttt tgcaatgaga gtcagtacgg cgttacgtat caaaccaaca
    41881 tcagccgtaa cggaactcca caccaaaggt gccgcgcggg tattcccact gttccagcgc
    41941 cgaccccagc cccagaatgc gacaggtgcc gcactccaga catccggcgt aatcgaagcg
    42001 cacactgccg tcatcctgct tcttgtacag acctgcgggg cacgctttca ccagcagctc
    42061 cagcacctgt ttatcagcat cggccttcac aacaatgtgc ggatgctctt catcgacatt
    42121 gaatttattg acgcccagtt tgacgtccac attgacggga gaagtcataa aacggtcact
    42181 cctttcatgc catccttgat cagattgatg aagcccactt tcttgccgtg gcggaggatt
    42241 ttcttgcgca tcagttccgg cgcactgccg tcaatggtga acaggtcacg cgccacgccc
    42301 accgccagtt ccgggttgcc gctaaacatg cgtgggttat caaggaacgc cggtagtttc
    42361 tggtacatgc gcatatcgcg cagcgggcca ctctccagat gctgacgata ttccgcgagt
    42421 ttttgcttac tgaaatcgtc gcttttcatc gctgaaagca cggtttttgc tgcagcttcc
    42481 ccggcggcaa tcgccagatc cataccgcga atggtaaaac cgaggttcat acacattccg
    42541 gcggcatcac cggcaatcaa tacgccgtca ccgaccagct ccggcagcat gttgatgcct
    42601 gcttccggca ctacgtgagc ggaatattcc accagcttgc cgcccgcgat cagcggtgca
    42661 acggccggat gctgtttgaa atcttccagc atttgcggca ccgatttttt cgcgtcatgc
    42721 agatgatgca gaccacaaac cagccccagc gacagggtat tttcattggt ataaaggaag
    42781 ccgccgccca tcaggccatc ggtgggtgat cccgcaaaca gacaagccgc gccctgatta
    42841 ccctgcaact gaaaacggtc ttcgattacc gacttcggta actcgatcag ttccttcacg
    42901 ccaaccgcca catccgtcgg tttaacgcgt tttgccatcc ccaacttttc ggcaaggatg
    42961 gagttcaccc catcagcaag gagcaccgtt ttcgcttcaa tcacatcacc atcggcttct
    43021 acaccgacga ctttgccatc gcgctgtacg aggttatcga cgcggatccc ggtaattaac
    43081 tgcgcgcccg cttcttcggc ctgctccatc aaccaggcat caaatttact gcgcaaaacg
    43141 gagtaagaac gctgggatgg cgaggtttcg tcaccgttgc agtagtccat agtcatcgct
    43201 gacttttccg tcataaacgc gagtttttca tgggtgatca ggcgttctac gggggcggaa
    43261 tcggcgaaac ctggaataat gtgttccaga ctgtgggcat agagacgccc gccagtaacg
    43321 ttcttggcac ctgcggaatt gccacgctcg ataactaaca cttgcgcccc ttcgcgggcg
    43381 agcaccagtg cggcaaccga accggcaagc cctgcaccga cgatgatggc gtcaaagata
    43441 tcttcggaca taagagctcc ctgtcagcgg cagagccgta atgcagccct gccagagtgg
    43501 atcaacgcgc taaagctgcg gtcagtgccg gaaggatctt cacggcatcg ccaacaatgc
    43561 cgtaatccgc gtactggaag atcggcgcat ttttatcttt attgatggcg aaaatggttt
    43621 gcgacgcgtt agcgccaacc atgtgctgga tctacccgga gatccccacc gccaggtaca
    43681 gttcaggttt cagcatcagg ttggagatac cgacatagcg ttcgtgctcc atccattttt
    43741 cgttttctgc caccggacga gaacaggcca actccgcacc tatcgccttg caaagctgtt
    43801 ctgccagcgc aatgttctct ttgctgccaa tgccgcgacc gacgctgacc accagacggg
    43861 ctttgtcgag atcgacgctg ttgctctggc gcgcctgggt tgccgtgcgg gtaatcgcca
    43921 cagccggagc ctgccactcc acggtgtgcg tttcgccagt gcgtgacgcg tctggctgag
    43981 ccgcatcgaa cgtgccgctg ctgatggtca gtaccgcata cggcgtggca atgcgttctt
    44041 cgccaatcgc cagaccgccg taaaccatgt gtttcactgt tgctttaccg tcctgtacgc
    44101 tgacggtgct ggcatcgtta gacaccgccg ctttaaggcg ataacccagt tttgccgcca
    44161 gtaatttgcc gcgacgggtg tttggcaaca gcaccagacc gtcagcgccg tgctggcgaa
    44221 tagtgtcagc catgacaccg gcgtaatctt cgatcatccg atcgtccggt ttgccgttta
    44281 atttccagac atgattagcg ccgagctgga ttgcctgtgc gccgtcggca tcattgagga
    44341 caaaggcatt gatttgatta gctaaagcct gcgcaccgtt catcagttcc ggcagacgag
    44401 agggggtatc gctgaatacc cagacttgag aaaacgtgtt catagcatcc cctgtaatta
    44461 aatgactttg cgaagatttt cagcaaatgc ggcgatctgt tcttcgccgt cgccttcaat
    44521 cacgatgcgc tgacgctcgc gctgtttcgg cgcggcaacc tgttgttcag accaggcgtc
    44581 cacggcgtta aaaccaatat ccgccgccga ccatacctgg actggctttt tcgccgcgcc
    44641 gagaatggct ttcatcgaag gaatttgtgg ggagttgata tcagtggaaa cagcaacaac
    44701 cgcaggcagc ggaatgctta aggtttcggt ttcatcttcc agttcgcgct caacggtgag
    44761 ggtatctgcc gtcagggaga taattttgct gacgccgtta actgccggaa tattgaggat
    44821 ttcgcccacc agcagaccaa cctgctgggc ataaaggtcg gaagaaccat cgccacagag
    44881 gatcagatca aagcctgctt tctgggcggc tgcagccagt gcgctcgccg tttgttgcgg
    44941 cagtgcctgc tcgaactggt catcaatcac cacaatcagt tcatccgggc cgcgcgatag
    45001 cacatcttta cgccctttgg cgttggtcag ggctttaccg cccacactta aggctgtcac
    45061 ctgcgcctct gctgcctgtt gctttagctg gcaagccgct tcaatagcgt tgagatcgta
    45121 ttggcttatt ttggcatcgg ctttgctgaa gtctaatgaa ccatcagcat tattgaccgc
    45181 aatatcctgt tcatcaggca cgcacttata gcaagtaata atcttcattg catctccaga
    45241 aatcatgaag gaaacgtcgt tcatatcggt ggtgttaatt acagatatca cgccctttaa
    45301 tttggaaata aaactttaat caccaatatt gaaagtcaga tgctattttt ttacccacaa
    45361 aagaatataa caaactgatt aataaccaaa attaacgaaa aacgccctct gttcactcga
    45421 aacaaaaatg tgataccaat cacagaatac agcttattga atacccatta tgagttagcc
    45481 attaacgcgt ccacgaggtt aataataatt atattaaatg ttaacaaaaa taaaacaaac
    45541 gggaacgcaa aataaatatt cgttttcaca gtggaattaa ctcatgaaga atgaaaagag
    45601 aaaaacggga atagaaccga aggttttctt tccgccatta ataatcgtcg gcatactttg
    45661 ttggcttaca gtcagagatc tggatgcggc gaatgtcgtt attaatgctg tattcagtta
    45721 cgtcaccaat gtatggggat gggcatttga atggtatatg gtggtgatgc ttttcggttg
    45781 gttctggctg gtgtttggcc cgtatgccaa aaagcgttta ggtaacgaac cgccagaatt
    45841 tagcaccgcc agttggatct ttatgatgtt cgcctcctgt acgtctgctg ccgtactgtt
    45901 ctggggatcg attgagatct actactacat ctccaccccg ccgtttggct tagaaccgaa
    45961 ctcgacagga gcgaaagagt tggggctggc ttacagcttg ttccactggg gacctctgcc
    46021 gtgggccact tacagcttcc tttcagtcgc cttcgcttac ttcttctttg tccgcaaaat
    46081 ggaagtgatt cgccccagct cgacactggt gccgctggca ggtgaaaaac acgccaaagg
    46141 gttgttcggt accatcgtcg acaacttcta tctcgtcgcc ttgatcttcg cgatgggtac
    46201 cagtctgggc cttgccacgc cgctggtgac cgagtgtatg caatggttgt ttggcattcc
    46261 gcataccctg caactggacg ctatcatcat tacctgctgg attatcctca acgccatttg
    46321 cgtcgcttgc ggtctgcaaa aaggggtacg tatcgccagt gacgtgcgta gttacctgag
    46381 cttcctgatg ctgggttggg tgttcatcgt cagcggtgcc agcttcatca tgaactactt
    46441 caccgattcg gtggggatgt tgctgatgta tctgccgcgc atgttgttct ataccgatcc
    46501 catcgctaaa ggcggcttcc cgcagggctg gaccgtgttc tactgggcat ggtgggtgat
    46561 ttacgccatc cagatgagta tcttcctcgc ccgcatctcc cgtggtcgta ctgtgcgtga
    46621 actgtgcttc ggcatggtga tggggctgac agcatccacc tggatcctgt ggaccgtgct
    46681 cggtagtaac actctgctgt tgatggataa aaacatcatc aacattccaa atctgatcga
    46741 acagtacggt gtggcgcgcg ccatcatcga aacctgggcc gctctgccac tcagcaccgc
    46801 caccatgtgg ggcttcttca tcctctgctt tattgccacc gttacgctgg ttaacgcctg
    46861 ctcttatacc ctggcgatgt ctacttgccg cgaagtacgc gatggtgaag aaccaccgct
    46921 gctggtgcgt atcggttggt caattctggt tggcattatc ggtattgttc tgctggcgct
    46981 cggcggcctg aaaccgattc aaaccgccat tatcgccgga ggatgcccgc tgttcttcgt
    47041 caacattatg gtgacgctct cctttattaa agacgcgaaa cagaactgga aagattaatt
    47101 aacccccaaa atatcaagag gttgaaagat ggattttaat ttaaatgatg agcaggaact
    47161 gtttgtcgcc ggtatccgcg aactgatggc cagcgaaaac tgggaggcct attttgccga
    47221 gtgcgaccgt gacagcgtct acccggaacg ttttgtcaaa gcactggcgg atatgggtat
    47281 cgacagtctg ctgatccctg aagagcacgg tggtctggac gcggggtttg ttactctcgc
    47341 cgccgtgtgg atggagctgg gacgtctggg ggcaccaacc tatgtactgt accagctgcc
    47401 gggcgggttc aataccttcc tgcgcgaagg cacacaagag cagatcgaca agattatggc
    47461 tttccgcggc accggtaagc agatgtggaa ctcagcgatt accgaaccgg gcgcgggctc
    47521 cgacgtgggt agcctgaaaa cgacttatac ccgtagaaat ggtaagattt atcttaatgg
    47581 tagtaagtgt tttattacca gtagcgccta caccccgtac atcgtggtga tggcgcgcga
    47641 cggggcttct ccggacaaac ctgtctacac cgaatggttt gttgatatga gcaaaccggg
    47701 catcaaagtg accaaacttg agaagctcgg tctgcgtatg gatagctgct gtgaaatcac
    47761 ctttgacgat gtggaactgg acgagaaaga catgttcggt cgggaaggta acggctttaa
    47821 ccgcgtcaaa gaagagttcg accatgaacg tttcctggta gccctgacca actacggtac
    47881 ggcgatgtgc gcctttgaag atgcggcgcg ctacgccaac cagcgcgtgc agtttggcga
    47941 ggctattggt cgtttccagt tgattcagga aaaattcgcc cacatggcga tcaaattaaa
    48001 ctccatgaaa aacatgctgt atgaagcagc gtggaaagca gacaacggca ccatcacctc
    48061 tggcgatgca gcgatgtgca aatacttctg cgccaatgcg gcatttgaag tggtggatag
    48121 cgcaatgcag gtgctgggcg gtgtcgggat tgcgggcaac caccgcatca gccgcttctg
    48181 gcgtgacctg cgtgtagacc gcgtttccgg aggatctgac gaaatgcaga tcctgacgct
    48241 gggtcgtgcg gtgctgaagc aataccgcta agtccgtgcg ctgcctgatg cgacgctgac
    48301 gcgtcttatc aggcctacgg gagcacgaat gtaagccgga taaggcgttt acgccgcatc
    48361 cggcagtcat gcgctacata aatttttcag gagctaataa tggatcatct acccatgccg
    48421 aaattcggac cgctggccgg attgcgcgtt gtcttctccg gtatcgaaat cgccggaccg
    48481 tttgccggac aaatgttcgc agaatggggc gcggaagtta tttggatcga gaacgtcgcc
    48541 tgggccgaca ccattcgcgt tcaaccgaac tacccgcagc tctcccgccg caatttgcac
    48601 gcgctgtcgt taaatatttt caaagatgaa ggccgcgaag cgtttctgaa attaatggaa
    48661 accaccgata tcttcatcga agccagtaaa ggtccggcct ttgcccgtcg tggcattacc
    48721 gatgaagtac tgtggcagca caacccgaaa ctggttatcg ctcacctgtc cggttttggt
    48781 cagtacggca ccgaggagta caccaatctt ccggcctata acaccatcgc ccaggccttc
    48841 agtggttacc tgattcagaa cggtgatgtt gaccagccaa tgcctgcctt cccgtatacc
    48901 gccgattact tttctggcct gaccgccacc acggcggcgc tggcagcact gcataaagtg
    48961 cgtgaaaccg gtaaaggcga aagtatcgac atcgccatgt atgaagtgat gctgcgtatg
    49021 ggccagtact tcatgatgga ttacttcaac ggcggcgaaa tgtgcccgcg catgagcaaa
    49081 ggtaaagatc cctactacgc cggttgcggt ctgtataaat gtgccgacgg ctacatcgtg
    49141 atggaactgg tgggcattac ccaaattgaa gagtgcttta aagatattgg cctcgcacat
    49201 ctactcggta cgccggaaat cccggaaggc actcagctta tccaccgtat cgaatgccct
    49261 tacggcccac tggtagaaga aaaactcgat gcctggctgg cggcacatac catcgcagaa
    49321 gttaaagaac gctttgccga actgaatatc gcctgtgcca aagtactgac cgtaccggaa
    49381 ctggaaagca atccacagta tgttgcccgt gaatcaatca ctcagtggca aacgatggat
    49441 ggtcgcacct gcaaagggcc gaacatcatg ccgaaattca aaaataaccc cggacaaatc
    49501 tggcgcggaa tgccctcaca tggcatggac acggctgcca ttttgaaaaa tatcggctac
    49561 agcgaaaacg acattcagga gttggtcagc aaaggtctgg ccaaagttga ggactaaacg
    49621 cgtggcgtca gtcggcttgc ctgactagcg cccgcccaat aacaaacgaa caaaaatgga
    49681 tagaggtgca atggatatca ttggcggaca acatctacgt caaatgtggg acgatctggc
    49741 ggacgtttac ggtcataaaa cggcgctgat ttgtgaatcc agcggcggag tcgttaaccg
    49801 gtatagttat cttgagttaa atcaggagat taaccgcacg gcaaacctgt tttatacgct
    49861 ggggattcgc aaaggcaaca aggttgcact acatctcgac aactgcccgg aatttatctt
    49921 ttgctggttc gggctggcaa aaattggcgc gattatggtg ccgattaacg cccgcctgtt
    49981 acgcgaggaa agcgagtgga tcttgcaaaa tagccaggcg tgcctgctgg tgaccagtgc
    50041 gcaattttat cctatgtatc aacagattca gcaggaagat gccactcaat tgcggcacat
    50101 ttgcctgaca gatgtggcac ttcccgctga tgatggagtg agttcattta ctcaactgaa
    50161 aaatcaacaa cctgccacct tgtgctatgc accgccgcta tcgactgacg atacggcgga
    50221 aattctcttc acctccggca ccacctcccg accgaaaggt gtggtgatta cccattacaa
    50281 cctgcgcttc gctggatatt actccgcctg gcagtgtgca ctgcgtgacg atgacgtcta
    50341 catgacggta atgcctgcgt ttcatatcga ttgccagtgt actgcggcga tggcggcgtt
    50401 ttctgccggg gccacctttg tgctggtcga gaaatacagc gcccgcgcct tctggggaca
    50461 ggcgcagaag taccgcgcca ccattaccga atgtattccg atgatgatcc gtacgttgat
    50521 ggtgcagccg ccttcagcga acgatcggca acaccgcctg cgggaagtga tgttttatct
    50581 caacttgtcg gagcaggaaa aagacgcatt ttgtgaacgc ttcggcgttc gcttgctgac
    50641 gtcttatggg atgacggaaa ccattgtggg cattatcggc gatcgcccta gcgataaacg
    50701 acgctggccg tcgattggtc gggcggggtt ttgctacgaa gcggagatcc gcgacgatca
    50761 caatcgcccg ctcccggcag gtgagatcgg tgaaatctgt attaaaggcg taccagggaa
    50821 aaccatcttc aaagagtatt ttctcaaccc gaaagccact gcgaaagtgc tggaagccga
    50881 cggctggcta cataccggcg ataccggata ccgcgacgaa gaaggctttt tttatttcgt
    50941 cgatcgccgc tgcaatatga tcaaacgcgg tggcgagaat gtctcctgcg tggagctgga
    51001 aaatatcatc gccacccatc cgaaaattca ggacatcgtg gttgtgggta ttaaagattc
    51061 gattcgcgat gaagccatca aagcatttgt ggtgctgaat gaaggtgaaa cattgagcga
    51121 agaggaattt tttcgcttct gcgaacaaaa tatggcgaaa tttaaagtgc cctcttatct
    51181 ggagatcaga aaagatctgc cacgtaattg ctcggggaaa ataattagaa agaatctgaa
    51241 ataaatgaaa cgtcagggaa ctaccctgcc agctaataat cacgccctaa aacaatatgc
    51301 attttttgca gggatgttgt cgtccctaaa aaagcaaaaa tggagaaaag gaatgagtga
    51361 atcattacat ctgacccgca atggatcaat tctggaaatt acccttgatc gcccaaaagc
    51421 gaatgctatt gatgcaaaaa ccagctttga aatgggcgaa gtatttctaa atttccgtga
    51481 cgatccgcaa ttacgtgtcg ccattattac cggtgccgga gagaaattct tttccgcggg
    51541 ctgggattta aaagcggcag cagaaggcga agcaccggat gctgactttg gcccgggtgg
    51601 ttttgcggga ttaaccgaaa ttttcaatct cgacaaacca gttatcgcag ctgtgaacgg
    51661 ctacgccttt ggcggcggtt tcgaactggc gctggcggca gattttattg tttgtgccga
    51721 taacgccagc ttcgccctgc cggaagccaa actgggcatc gttcctgaca gcggcggtgt
    51781 gctgcgtctg ccgaagatcc tgccgcctac catcgtcaat gaaatggtga tgaccggcag
    51841 acgaatgggc gcagaagagg cgctgcgttg ggggatagtc aaccgcgtgg ttagccaggc
    51901 ggaactgatg gataacgccc gcgaactggc tcagcagctg gttaacagcg ccccgctggc
    51961 gattgcggcg ctgaaagaga tctaccgcac caccagcgaa atgccggtag aagaagcgta
    52021 tcgctatatt cgcagcggcg tgttgaaaca ctatccatcg gttctgcatt cggaagatgc
    52081 cattgaaggg ccgctggcgt ttgccgagaa gcgcgatccg gtgtggaaag gacgttaaca
    52141 accatgagct attacgcctt tgaggggtta attccggtgg ttcacccgac ggcgtttgtc
    52201 catcccagtg ccgtcttgat tggcgatgtg attgtgggag ccggtgtcta catcggccca
    52261 ctcgcctcac tgcgtggtga ctacgggcgg ttgatcgtgc aagcgggagc caatattcag
    52321 gatggctgca ttatgcatgg ctactgcgac actgacacca tcgttgggga aaacggccat
    52381 atcgggcatg gagcgatcct gcatggttgt gtgattggtc gcgatgcatt ggtcgggatg
    52441 aacagcgtaa ttatggatgg cgcggtcatt ggcgaagaga gcattgttgc cgcaatgagc
    52501 tttgtcaaag caggctttag tggcgagaaa cgccagttgt tgatgggtac gccagcccgc
    52561 gctgtacgca gtgttagtga cgacgagtta cactggaaac ggttgaatac caaagagtat
    52621 caggatcttg tcgggcgctg tcatgcatcg ttacatgaaa cgcagccact gaggcaaatg
    52681 gaggaaaatc gcccccgttt gcaggggacg acggatgtga cgcctaaacg gtgatcttgc
    52741 cggatgcgac ctggttgctg gataagatgc tacaacatcg tatctggcaa gcaggacgcc
    52801 cggtatcaac gagataaaat taacgacaca tactctttga caacatcatc tgccacttct
    52861 gctcgcggtt aagttccgtg agcggaacgg caggaatacg cggtgtcttt cttacgccga
    52921 ctaatttttc ccgactgtta ttgcgcaggc gcgcgtaaat ttgctcatcg atatcgacca
    52981 ctttaaccag tcgctgacac tggcatcccc gcccttccag cttattaggg atcatcttaa
    53041 cttcgcagct tatttctgtg acttccgaca gaatataagt cagggtatta accgccttgc
    53101 tgtgttcaat atcgaaatga atagagatct ctcttgctat cacccaccga ttttcagcca
    53161 tcatccattc ggcgattaac aagtagagtg gtttttcaac atatccttca cacatacgaa
    53221 ctccagtttc acaaattaaa agtctagcta tgactcgttt tgaagcaatt aaacaaggcc
    53281 atattaaaat tgtggatata tcgatagtat gcaacttcac cgtcgataag tgtgagctaa
    53341 atcctgctta tgtcatcaaa aatatcgatt ccccaaaaga tttacttaat gggcaaaaaa
    53401 aaacggtcct catcagagaa ccgtattagt tattcgatca aattagcgga tgaaaaatat
    53461 ccgccacgac acgctattat ttgatctgtg cgtgcatttc ctgcaccgaa attacttttt
    53521 cagtcgcatc ggcattcagc gccatcgcgg tagcgaaacc accgttcagg gtggtgtcat
    53581 aatgcacttt atattgcagc gcactgcgac ggatcacgcg ggagtcttca atcgcacgac
    53641 ggcctgaggt ggtgttgatg atgtaggtat attcgccatt cttgatacgg tcctgaatgt
    53701 gcggacggcc ttcatgcacc ttgtttacca gacgcggatt gatacccgct tcgcccagca
    53761 caatcgccgt gccgtgggtc gcatccagct cgaagccctg tttcagcagt tttgccgcca
    53821 ggtccaccac gcgttcttta tcgccttcgc gcacggaaag cagcgcacga ccgtgtttct
    53881 tcatggtgga gttgctgccc agctgcgctt tggcgaacgc ttcagcgaag gtgcggccca
    53941 cgcccatgac ttccccggta gagcgcattt ctggccctaa cagcgggtca acgcctggga
    54001 atttattgaa cggcagcacc acttctttca ccgagtagta cggcggaata acttctttgg
    54061 ttacgccctg ctcagccagc gatttgccag ccatcacacg cgccgccact tttgccagcg
    54121 gtacgccggt ggctttggag acgaacggaa cagtacgcgc cgcacgcggg tttacttcaa
    54181 tcaggtaaac ttcgttgttt ttcaccgcaa actgcacgtt catcagaccg cgcacctgca
    54241 attcgaaggc cagtttctgc acctgctggc gcatcacatc ctgaatttcc tgacttaagg
    54301 tgtacgctgg cagcgaacat gcggagtcac cggagtgcac acccgcctgc tcgatgtgct
    54361 ctatgatgcc gccaatcagc accatttcgc cgtcgcagat ggcatccacg tcaacttcta
    54421 ctgcgtcatc aaggaaatga tccagcaaca ctggcgcatc gttagacacg ctaaccgccg
    54481 tctggaagta gcgacgcagg tcagcttcgt catagacgat ttccatcgcc cgaccgccga
    54541 gaacgtaaga cggacgcacc accagcgggt agccaatctc ttttgccttc tcaactgcca
    54601 tttcaatagc agtaacggtg gcgttcgccg gttgtttcag tttcagacgg tcaaccgcat
    54661 gctggaagcg ttcacggtct tccgcacggt cgatagcatc cgggctggtg ccgataaccg
    54721 gtacgccagc ggcttccagt gcacgcgcca gtttcagcgg ggtctgaccg ccgtactgaa
    54781 cgataacgcc tttcggcttc tcaatacgca cgatttccag cacatcttcc agagttaccg
    54841 gctcgaagta gaggcgatcg gaagtgtcgt agtcggtaga gacggtttcc gggttacagt
    54901 taaccataat ggtttcgtaa ccgtcttcac gcagcgccag cgaggcgtgt acgcagcagt
    54961 agtcgaattc gataccctga ccgatacggt ttggaccgcc gccaagcacc atgatttttt
    55021 cacggtcggt agacggattc gcttcgcact cttcttcata agtggagtac atgtaagcgg
    55081 tgtcggtggc aaactctgcc gcacaggtat ccacgcgctt gtagaccggg tgcaggtcat
    55141 attggtcacg cagcttacgg atttccgctt cgcgtacgcc cgccagtttt gccaagcgcg
    55201 catcggcaaa gcctttgcgt ttcagctggc gcaggaattc agcgttcagg ccagtgatgc
    55261 ccacttccgc cactttctct tccagacgca ccagttcttc aatctgtacc aggaaccagc
    55321 ggtcaatgtt agtcaggttg aagacgccgt ccacagacag gcccgcgcgg aaagcatcgg
    55381 cgatgtacca gatacgctct gcgcctgcgt ctttcagttc gcgacggatt ttggttaacg
    55441 cttccgggtc atccaggctc actttcgggt cgaagccagt tgcgcccact tccaggccgc
    55501 gcagcgcttt ttgcagggat tcctgctgcg tgcgaccaat cgccatcact tcgccaaccg
    55561 atttcatctg agtggtcaga cggtcgttag caccggcgaa tttttcgaag ttgaagcgag
    55621 gaattttagt aaccacatag tcgatggacg gctcgaagga ggccggagta cgtccgccag
    55681 tgatgtcgtt catcagttcg tctagggtgt aacccaccgc cagtttcgcc gccactttag
    55741 caatcgggaa accggtcgct ttcgacgcca gcgccgaaga acgggacacg cgtgggttca
    55801 tttcgataac aatcaggcga ccgtttttcg ggttcaccgc aaactggaca ttggaaccac
    55861 cggtttcaac gccgatttca cgcagcaccg ccatcgaggc gttacgcatg atttgatatt
    55921 ctttgtcggt cagcgtttgg gctggcgcga cagtgatgga gtcaccggtg tggatgccca
    55981 tcgcatcaaa gttttcgata gagcagacga tgatgcagtt gtcgttttta tcacgcacca
    56041 cttccatctc gtactctttc cagccgatca gcgactcatc aatcagcaac tctttggtcg
    56101 gagagagatc cagaccgcgg gcgcaaattt cttcaaactc ttcacggtta taagcgatac
    56161 cgccgccgct accgcccatg gtaaaggatg ggcgaataat gcacgggaag cccacgtcag
    56221 cggcaacagc cagcgcttct tccatcgtgt gtgcgatacc ggaacgcgcg gtttccagac
    56281 caattttctt catcgctacg tcgaaacgac ggcggtcttc tgctttatca atcgcatcgg
    56341 cagtggcacc aatcatggtg acaccgaact cttccaacac gccctgacgt tccagctcca
    56401 gcgcgcagtt cagcgccgtc tgaccgccca tggttggcag caccgcgtcc gggcgctctt
    56461 tttcaataat cttgcgtacc acttcccagt gaatcggctc gatgtaggtc gcatcggcca
    56521 tttccgggtc ggtcatgatg gtcgccgggt tggagttcac cagaatgacg cggtaaccct
    56581 cttcgcgcag ggctttacat gcttgcgcgc cagagtagtc aaactcacac gcctgaccga
    56641 taacaatcgg gcccgcaccc agaatcagga tactttttat atctgtacgt tttggcatgg
    56701 ctcttttact cctgattact tagcggtttt acggtactgc tcaattaact tgataaagtg
    56761 gtcgaacaac ggcgcggcgt cgtgtggacc agggctggct tcagggtgac cctggaagct
    56821 gaacgccggc ttatcgatgc gatgaatgcc ctgtaacgta ccgtcgaaca gggatttatg
    56881 cgtgacacgc aggtttgcag gtaatgttgc ttcatccacc gcaaaaccgt ggttctgggc
    56941 ggtgatcatc accacgtttt tctccacatc tttaaccgga tggttgccgc cgtggtgacc
    57001 aaatttcatt ttgacagtct tcgcaccgct cgccagcgcc agcagctgat gaccgagaca
    57061 gatgccgaat accggaatat cggtttcgag gaacttctgg atggcggtaa tggcgtaatc
    57121 gcacggggcc gggtcgccag gaccgttgga gaggaagatg ccgtctggat tcattttcag
    57181 cacatcttcc gcagaagttt gcgccggaac gatggtcagg cgacagcctc tatccaccag
    57241 catccgcagg atgttgcgct tggcaccaaa atcataagcc acgacgtgga acggcagctc
    57301 gtcttctttt ttcgcttctg gcaggccgcc ggtcaacgtc cagctccctt gtgtccagct
    57361 ataggcttct gcggtggtca cttcttttgc cagatccatg ccgttcagac ctgggaacgc
    57421 gcgggctttt tctaacgcca gcgccgcatc cgggttatcg cctgcgataa tgcagccatt
    57481 ctgtgcgcct ttctcgcgca gtaaacgcgt cagcttacgg gtatcgatat cggcaatcgc
    57541 cacgatgtta tggcgcttca ggtaagaaga gaggtcttcg gtattacgga agttgctggc
    57601 aatcagcggc aggtcgcgaa tcaccagacc ttgtgcatgt acctgagaag attcttcatc
    57661 ggcgtcatta gtgccgacat tgccaatatg gggataagta agagtaacga tttgacgaga
    57721 ataggaagga tcagtgagga tttcttgata accggtcatt gaagtattga aaacgacttc
    57781 cccaaccgcc gaacctgttg cccctatggc ccgaccgtga aactgggttc cgtcttccag
    57841 aaccaatagc gctgacttaa tcaaaacacc ctccagagaa tactcactca ctttatttgc
    57901 atattaattc acagtgatga cgtaaatcaa tgcaaatctg cttaccgacg aatttctggc
    57961 aaacggcggc attctggaga tatgggcgct aaaagtcaac ttaatggcag gattttttat
    58021 attattttgt ggctcatgca tgttttacag gcattaagcg acaaaaacat catctaacct
    58081 gttctggaaa acgcttgcgc agcaagaaga ttgcatgaaa taaaaacgag caggaaaaag
    58141 tggaccaaat ggtcaaaatt tacattaaaa caacaaaaat agaagacgaa acatgtaatt
    58201 tttggcagtt atagaaaatt taaaagcata acaccaaaaa taaaaggact aaaaattaaa
    58261 gccctttata attaataaat taaatgtgtt acttttgcat cataataaac attttgtggt
    58321 tacaaattat tgagatcaag cacatctcgc atatcaaaaa gaccactttc cttaccactc
    58381 aaccacaaag ccgatcttac cgcaccgtta gcaaatgtca tgcggctgga cgccttatgg
    58441 gtgatctcca gacgctcgcc aatatcggca aacatcgcgg tatgttcgcc aacgatgtca
    58501 cctgcacgca cggtagcaaa accaatggta cctggcacac gttcaccggt atggccttca
    58561 cgactgtaga ccgcgcaatc tttcagatct ttatcaaggg cgtgggcgat cgcctctccc
    58621 attgccagtg cggtgcctga cggcgcatca actttatgtc tatgatgtgc ttcaataatt
    58681 tcgatatcgg tgtagtcacc catcactttg gctgctttct ccagcagctt aagcatgacg
    58741 ttaacgccaa cgctaaaatt ggcggcaaag acaatcgcaa tatcggcggc ggcgtcacga
    58801 attgcctgtt taccggcttc gtcaaacccc gtagtgccga tcaccatccc tttgccatgc
    58861 tgccgacaaa aagcgagatg gttcagcgta ccttccggac gggtaaaatc gataaacaca
    58921 tcaaaatcat cttttatcgc atcaagactg ctttgcacgg taacgcctgt tttccctgca
    58981 ccagccagct caccggcgtc gctgcccagt aaagaagatc cttcgcgctc cagcgccgcg
    59041 cccagctgca cgccttctaa tgccagtgcc gcctgaatca actggcggcc catacgcccc
    59101 ccggctcccg cgatggcaac gcggatgttt gcatcatgca tagctattct cttttgttaa
    59161 tttgcataga ccgttttcag agtaaccagc gcatcgctac gccgccagcc aaaaacgctg
    59221 ataattagaa attaatgata aacagggagg gatatcagtg aaaggcatga gtcgatgaat
    59281 gactgcatgc cgataacatg tgacaggtta cggcaccaga gccagcacct cagccaccca
    59341 ctgttggaag ccttttacat tcagatccaa tgccacctgt acattggctg gtttgcccag
    59401 gcaaccgtcg atatcaacca ccgtcgtgcc tgaggtaaat tcgccttgag tttctaccgc
    59461 gacaaaacac ggcttgaggg tgaataagtc cgggcgcacc agccaggcga tggcgcagag
    59521 atcgtgcatt cgcagaccgc tttgcatact gccgctacgg tagtggctaa acagggcgtg
    59581 gagcattttt ccggtgcgat tcaactccgg taaagtagcg agatagtcag gggttaatat
    59641 tgcctgattg gtgacatcca gaccgcacat gacaatttcg atgccgctac ggaagacaca
    59701 ggcagccgct tctggatcgg cagcgatatt aaactcggca tttggtgtac agttgccgcg
    59761 accagcagaa ccgcccatga tcaccagacg acgaatatac ggtttacatt ccgggcattg
    59821 taaaagtaac agcgcaatat tggttaacgg gccgatggcc accagggtaa cgggctctgg
    59881 tgcacgcatc agggcatccc gaatcgccag aaacgccggt atcccgagtg gctttcggtt
    59941 gtgctcaaca aagtcgtagc cagccattcc cgattcgccg tgcacagatg ctgcatcacg
    60001 cggtgcgcgt accagcggca cagcggctcc ctgggcgagc ggaatctccg cattccagaa
    60061 atgcagcagt tgcagggcat tgcgggtagt tttctcaacc gagacattac ccgcgacggt
    60121 ggtcatcagt tgcaggtcga gttcgggggc aaaaatcgcg gcggcaatgg cgacggcatc
    60181 gtcaatgccg gggtcagtat cgaggaagat aggtaaacgc atgttttctt cataaaaaat
    60241 gccggtaaca agaccggcat tttcgcataa cttaggctgc taatgactta atcgacttca
    60301 cgaatatcga cacgcagctc tttcggcact tcgaaaacaa tattttcttc acggccttcc
    60361 agcggaatgg cttcaccacc acccaactgc tgcaaacgtg ccaccacatt ctgcaccaga
    60421 atatccggag ccgatgcgcc cgcagtcacg ccgacgcatt taacctcttt cacccactct
    60481 tcctggatat ctttcgcatc gtcaatcaaa aacgcgtgtt tgcccatacg ctgggccagc
    60541 tccgccagac ggttggagtt ggaggagttt ttcgaaccga ccaccaacac aacttccgcc
    60601 tgttctgcca gggcgcgtac cgcttcctga cggttagtcg tggcgtagca gatgtcatct
    60661 ttgcgcggac cgacaatttt cgggaagcgt ttacgcagcg cgtcgatcac atcagacgtg
    60721 tcatccaccg acagcgtggt ttgggtcata aaggagagct tctcttcgtt tttgaccgtc
    60781 agtttccaca catcgtctgg cgattcgact agatacattc ccccttcggg gttgctgtac
    60841 tgccccatcg tcccttccac ttccgggtgc ccggcgtggc cgatgagaat agattcttcg
    60901 ccacggcgac tggcgcgggc aacttccata tgcactttgg tcaccagcgg acaggtggca
    60961 tcaaacaccg tcaaatcgcg gcttttcgct tcgttacgaa ccgcctgaga aacaccgtgt
    61021 gcggagaaaa tcaggatcgc gccgtccggc acttcgctaa tctgctcaat aaagatagcc
    61081 ccacgctcgc gcaagctatc gaccacgtag cggttatgca ccacttcgtg acggacatat
    61141 atcggtgcgc cgtaaatggc cagcgcgttt tcaacaatgc tgatagcgcg gtctaccccg
    61201 gcacaaaaac cacgcgggtt ggccaacagg atctgcatgt tacgcctcca gtgccggatc
    61261 gatttccagc acttcaatat caaaatgaac ggtctgcccg gccagcggat ggttgaaatc
    61321 aacggtaatg gagtcgccgt taatttcgcg gatcacacca ggcatctcac tgccatccat
    61381 tgcggtaaaa agcatgattg cgccaatttc tggctcgcct gcatccataa attcacggcg
    61441 ggagaagtac tgaatcaggt ccggtgacgg cacgccaaac gccgcatctg gctccagcga
    61501 gaaggtggtt ttgtcgccca ctttcagccc cagcaggtgt tgctccagcc cttcagaaag
    61561 agaagcatcg cccaggcgga acagcgccgg tttaccgttg ttgcgagtag actcggcggt
    61621 ggtgccatcg tcgagtttta gcgtgaagtg caccaggacg gcgctattgc tctgtacaga
    61681 ttcagacatg caggtttgct cttatttttg aggatttttg ccggatgcgg cctacgattt
    61741 cgtagcaatc tgtaggccgc atccggcagg atttattatt gttttttcgc tttggaaggt
    61801 aaaaaccctt ccagcacgat cagtgccgca ccgacacaaa tggcagtatc ggcaaggttg
    61861 aaggtggcga agtgccagtc gccgacgtag aagtcgatca tatcgacaac gaagccgtgc
    61921 cacaggcggt cgaacaggtt gcccagcgcg ccgccaataa tcagcgcgta agcgatattg
    61981 tttagcttct gcgtggcctt cgagcgatac atcatcactg ccaggagcac gctaatacca
    62041 atcgcaatac cggcaaagaa ccaacgctgc cagccgccgc tatcggcaag gaaactaaac
    62101 gccgcgccat agttacgcgc ataatgcaga ttaagcgacg ggaacagcgg gaccgtatcc
    62161 cccagagcaa agttctggag gatcaggtat ttgctgccca gatcgataat cagcacgact
    62221 accaccagcc acagccagcg tagccctgtt gaacagatcg attgactcat caggcaaact
    62281 tacgtttttc accgtcaccg gcgacgttgc tgacacagcg gccgcagatt tctgcgtgtt
    62341 ccgccacctt gccgacatcc tgggtgtagt gccagcagcg tgggcacttc tcaccttcgg
    62401 ctttactcaa cgcgactttc agccctttga gtacttcgct ctgctgagca tcagcaggtg
    62461 cgtcgttata gtctgcaacg gtagcgccgg aggtcaacag gacaaatcgt aattcatcgc
    62521 ccagcgcggt cagtttcgcc gccagttccg gttctgcata caaggttact gccgcttcca
    62581 gcgagccacc cactttcttg tcggcacgcg cttgctcaat gactttgttc acttcgccac
    62641 gcactttcaa cagctcgtcc cagaacgcat cgttcattgc ttcactgtct gccagaccaa
    62701 acaggccttc gtaccactca ccggtgaaga cgtatttttc acgttcgccc ggcagatagc
    62761 cccacacttc atcagcggtg aaggagagga ttggtgccat ccagcgcacc agcgcttcag
    62821 cgatgtgata cagcgcagtc tggcagctac gacgcgccac gctatccgct ttggcggtgt
    62881 actgacggtc tttgatgatg tcgaggtaga aggaacccat ctcaacggag cagaagcgca
    62941 tcagacgctg taccacttcg tggaaatcgt atgcttcgta cgccttgagg atgtcttcct
    63001 gtgccgcttt cgcacaacct acggcccagc gatccagtac caccatctct tccggtttca
    63061 ccatatcttt tgctggatca aaaccattca ggtttgccag caggaagcgc gcggtgttac
    63121 ggatacgacg atagctatcg gcagcacgtt tcaggatctc gtcagaaacg gccatttcac
    63181 cggtgtagtc ggttgatgcc acccacagac gcagaatatc cgcgcccagt ttgttcatca
    63241 catcctgcgg cgaaacggtg ttgccgatgg atttagacat cttgcgaccc tgaccatcca
    63301 cggtaaagcc gtgggtcagt acctgacgat acggcgcttt gcctttcatc gcggtggaga
    63361 tcattaggga agacatgaac caaccacggt gctggtcaga accttccaga tacatgtccg
    63421 ctgcgtgacc ggcaaattcc ggacgcacgt caacaacaga agagtgggta gatccggagt
    63481 caaaccatac gtcgagagta tccggcactt tcacatactg gtcagcttcg tcgccgagga
    63541 tctctttcgc atcgagatcc caccacgcct ggatgccgtc aacttcaacg cgtttagcca
    63601 cttcttccat cagttcgagg gtacgcggat gcagctcttc cgtgtctttg tgcacgaaca
    63661 gtgacatcgg tacgccccag gtgcgctgac gggagataca ccagtcagga cggttagcaa
    63721 ccatcgactc gatacgcgcc tggccccagt ccgggagcca ctgcacgcct ttgatctctt
    63781 tcagtgactg cgcacgcaga cctttctgat ccatgctgac gaaccactgc ggtgtcgcgc
    63841 ggaagatgat cggcgttttg tgacgccagc agcacggata gctgtgctgc attttctcaa
    63901 cgtgcagcag cgcgcctttt tcctgcagca gcgcaacgac gatgtcgttc gctttgaaga
    63961 cgttcacgcc atccagcgtc ggataagtgc ccggcagata agtgccgtcc gggccaaccg
    64021 ggttagcggt ttccaggccg tatttctgac cgatcacata gtcgtccggg ccgtggccag
    64081 gcgcggtgtg aacggcaccg gtaccggcat ccagggtaac gtgatcgccg aggattgccg
    64141 gaacgtcgaa gcccataaac ggatgggtaa agcgcagcag ctcaagctct gcacctttta
    64201 ccgtgccgag aatggtgtaa tcggtcacgc cgatacgctg cattacgctt tcaaccagat
    64261 ctttcgccag aatcacggcc tgaccgtcga tctgcaccag cgcatagtcg aaatcaggtg
    64321 caatagagat tgcgcggttc gcaggcagag tccacggcgt agtggtccag atcaccagcg
    64381 agattgggcc gttaacgttg cttacgccaa atttcgcttt cagcgcatcc tgatcgaccg
    64441 cctggaaagc gacgtcaatg gacggagaag ttttgtcgta atactcaact tccgcttctg
    64501 ccagtgcaga acggcagtct acgcaccagt gcaccggctt cgcgcctttg tgcaggtgac
    64561 cattgccgat gattttgccc agcgcgcgga tgatgttggc ttcagttttg aagtccatag
    64621 tcagatacgg gtgcgaccag tcgcccagca cgcccagacg gataaagtct ttgcgttgac
    64681 cgtcaacctg ggtcgccgcg tattcgcggc acttggcgcg gaactcggcg gcggtgaatt
    64741 tctcacccgg cttaccgtat tcttgctcta ctttcagctc gatcggcaga ccgtggcagt
    64801 cccagccagg cacatacggc gagtcataac cggaaagccc tttggacttc acgataatgt
    64861 ctttcagaat cttgttaacc gagtgaccaa tatgaatgct gccattcgca taaggagggc
    64921 catcatgcag aatgaaggtt tttttgcctt ttttagccgc acggatgatg ccgtacagat
    64981 catcatcagt ccaacgcgcc agcattcccg gttcgcgctt ggcgagatcg ccacgcatcg
    65041 ggaaccctgt ttccggcaaa ttcagggttg atttatagtc actcatcaga ttctcggttc
    65101 cgtgtttcgg tttgattaca taacaggctt aagccggttt tgttagccca aaaaattcgc
    65161 gggcggttaa ttcatcacgc gcaatctgcg ctttcagttc gtccagcgac gcaaatcgct
    65221 gctcattgcg tattttttta cgcagcacta cttgtatatg gcgaccgtaa aggtccattg
    65281 caacatctaa caaatgcact tccagctgct ggcgaatacc ggcaaccgtt gggcgtgttc
    65341 cgatgtttgc cacgccgggt aacggctttt caccgaggcc cagcacttct accgcataaa
    65401 cccctttcac cggggaaacc tgacggcgta gcggtacatt cgccgtcggg aaacctatag
    65461 tgcgccctaa ttcatcaccg tggactacac gcccggagat ggcaaacggg tgccccagta
    65521 aactttctgc cagagccaga ttgtcatcag ccagcgcctg acgcacggcg gtgctgctga
    65581 tacgcacacc accttcgcaa aaagtttgcg tactggtgat atcgaagccg tattccatgc
    65641 cagctttctg taataacaag aaatcgcctt cacgaccagc gccaaagcgg aaatcatcac
    65701 ctacggcaag aaattttacg cgcaaatgct tcaccaaaag atcgctgatg aaattttgcg
    65761 cggttaacgc cgcaaaacgc ctgtcgaaac gcacgcacag cacgtaatca acgccacact
    65821 ctgcaaggta acgcagtttt tcccgcagcc gggtcagtct tgccggggct ttatcggtag
    65881 caaacagttc cagtggttga ggttcaaaaa gcatcaccat caccggtaag ttgcgcttgc
    65941 gcccttcttc ctgcaagccc tgtaacagcg cgcgatgacc gcgatgcacg ccgtcgaaat
    66001 taccaatagt cagcacacac ccttcttgcg gggcctggct gagattatgt atgccgcgta
    66061 tcagcttcat gtctggctca aaacagtgaa aatcgtccga gtataccttg tacagcggtc
    66121 aaggttaacc ggcgattgag taccgaaatc agaaagaagt ggcttttcat tgccatggag
    66181 caaatcacgg gaagaaactg accgcctgct gcaattttta tcgcggaaaa gctgtattca
    66241 caccccgcaa gctggtagaa tcctgcgcca tcactacgta acgagtgccg gcacattaac
    66301 ggcgcttatt tgcacaaatc cattgacaaa agaaggctaa aagggcatat tcctcggcct
    66361 ttgaattgtc catatagaac acatttggga gttggacctt ggctaatatc aaatcagcta
    66421 agaagcgcgc cattcagtct gaaaaggctc gtaagcacaa cgcaagccgt cgctctatga
    66481 tgcgtacttt catcaagaaa gtatacgcag ctatcgaagc tggcgacaaa gctgctgcac
    66541 agaaagcatt taacgaaatg caaccgatcg tggaccgtca ggctgctaaa ggtctgatcc
    66601 acaaaaacaa agctgcacgt cataaggcta acctgactgc acagatcaac aaactggctt
    66661 aatcgccaat ttgctgaagc tttgtgaaaa agcccgcgca agcgggtttt tttatgcctg
    66721 ctgcttttgt gatgcgtgag agaacacact gccggttgcc agatagtcaa aagagacatg
    66781 ccgttgtact atctgaactc ttcgtggctt aagaatcgta gaaccatcgg ctgatacatt
    66841 ctgaacaaga atatctactc aatctccaga ctcccccaca aagaatatgg atattgtgat
    66901 acacattgag gtattaaact taagaccact ctatttttga attaaattcc tatatacttt
    66961 aaccattcat aaatatctcc tcattagacc tt
//
LOCUS       DAJTRD010000009        66630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTRD010000009 DAJTRD010000000
VERSION     DAJTRD010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.009"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO315_000655"
     CDS             complement(32..2479)
                     /gene="glgP"
                     /locus_tag="OO315_000655"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5889683.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO315_000656"
     CDS             complement(2498..3931)
                     /gene="glgA"
                     /locus_tag="OO315_000656"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5889684.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO315_000657"
     CDS             complement(3931..5226)
                     /gene="glgC"
                     /locus_tag="OO315_000657"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5889685.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO315_000658"
     CDS             complement(5244..7217)
                     /gene="glgX"
                     /locus_tag="OO315_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5889686.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO315_000659"
     CDS             complement(7214..9400)
                     /gene="glgB"
                     /locus_tag="OO315_000659"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5889687.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO315_000660"
     CDS             complement(9673..10776)
                     /gene="asd"
                     /locus_tag="OO315_000660"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5889688.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10968..11561
                     /gene="yhgN"
                     /locus_tag="OO315_000661"
     CDS             10968..11561
                     /gene="yhgN"
                     /locus_tag="OO315_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5889689.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO315_000662"
     CDS             complement(11618..12958)
                     /gene="gntU"
                     /locus_tag="OO315_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5889690.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO315_000663"
     CDS             complement(12962..13489)
                     /gene="gntK"
                     /locus_tag="OO315_000663"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5889691.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO315_000664"
     CDS             complement(13628..14623)
                     /gene="gntR"
                     /locus_tag="OO315_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5889692.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO315_000665"
     CDS             complement(14847..15542)
                     /gene="yhhW"
                     /locus_tag="OO315_000665"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5889693.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15665..16702)
                     /locus_tag="OO315_000666"
     CDS             complement(15665..16702)
                     /locus_tag="OO315_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5889694.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17035..17523
                     /gene="yhhY"
                     /locus_tag="OO315_000667"
     CDS             17035..17523
                     /gene="yhhY"
                     /locus_tag="OO315_000667"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5889695.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17582..19312)
                     /gene="ggt"
                     /locus_tag="OO315_000668"
     CDS             complement(17582..19312)
                     /gene="ggt"
                     /locus_tag="OO315_000668"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5889696.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSRIITTVLQMVVNSIDYGMNVAEATNAP
                     RFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASDP
                     RSVDDLTAGY"
     gene            19432..19872
                     /locus_tag="OO315_000669"
     CDS             19432..19872
                     /locus_tag="OO315_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5889697.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19859..20602)
                     /gene="ugpQ"
                     /locus_tag="OO315_000670"
     CDS             complement(19859..20602)
                     /gene="ugpQ"
                     /locus_tag="OO315_000670"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5889698.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20599..21589)
                     /locus_tag="OO315_000671"
                     /pseudo
     CDS             complement(20599..21589)
                     /locus_tag="OO315_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21591..22436)
                     /gene="ugpE"
                     /locus_tag="OO315_000672"
     CDS             complement(21591..22436)
                     /gene="ugpE"
                     /locus_tag="OO315_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5889699.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22433..23320)
                     /gene="ugpA"
                     /locus_tag="OO315_000673"
     CDS             complement(22433..23320)
                     /gene="ugpA"
                     /locus_tag="OO315_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5889700.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23418..24734)
                     /gene="ugpB"
                     /locus_tag="OO315_000674"
     CDS             complement(23418..24734)
                     /gene="ugpB"
                     /locus_tag="OO315_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5889701.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25131..25844)
                     /gene="livF"
                     /locus_tag="OO315_000675"
     CDS             complement(25131..25844)
                     /gene="livF"
                     /locus_tag="OO315_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5889702.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25846..26613)
                     /gene="livG"
                     /locus_tag="OO315_000676"
     CDS             complement(25846..26613)
                     /gene="livG"
                     /locus_tag="OO315_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5889703.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26610..27887)
                     /gene="livM"
                     /locus_tag="OO315_000677"
     CDS             complement(26610..27887)
                     /gene="livM"
                     /locus_tag="OO315_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5889704.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27884..28810)
                     /gene="livH"
                     /locus_tag="OO315_000678"
     CDS             complement(27884..28810)
                     /gene="livH"
                     /locus_tag="OO315_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5889705.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28858..29967)
                     /gene="livK"
                     /locus_tag="OO315_000679"
     CDS             complement(28858..29967)
                     /gene="livK"
                     /locus_tag="OO315_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5889706.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30391..30774
                     /gene="panM"
                     /locus_tag="OO315_000680"
     CDS             30391..30774
                     /gene="panM"
                     /locus_tag="OO315_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5889707.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30973..32076)
                     /gene="livJ"
                     /locus_tag="OO315_000681"
     CDS             complement(30973..32076)
                     /gene="livJ"
                     /locus_tag="OO315_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5889708.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32347..33201)
                     /gene="rpoH"
                     /locus_tag="OO315_000682"
     CDS             complement(32347..33201)
                     /gene="rpoH"
                     /locus_tag="OO315_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5889709.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33446..34504)
                     /gene="ftsX"
                     /locus_tag="OO315_000683"
     CDS             complement(33446..34504)
                     /gene="ftsX"
                     /locus_tag="OO315_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5889710.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34497..35165)
                     /gene="ftsE"
                     /locus_tag="OO315_000684"
     CDS             complement(34497..35165)
                     /gene="ftsE"
                     /locus_tag="OO315_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5889711.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35168..36664)
                     /gene="ftsY"
                     /locus_tag="OO315_000685"
     CDS             complement(35168..36664)
                     /gene="ftsY"
                     /locus_tag="OO315_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5889712.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36814..37410
                     /gene="rsmD"
                     /locus_tag="OO315_000686"
     CDS             36814..37410
                     /gene="rsmD"
                     /locus_tag="OO315_000686"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5889713.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37400..37669
                     /locus_tag="OO315_000687"
     CDS             37400..37669
                     /locus_tag="OO315_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5889714.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37672..38031)
                     /locus_tag="OO315_000688"
     CDS             complement(37672..38031)
                     /locus_tag="OO315_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5889715.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38172..38798
                     /locus_tag="OO315_000689"
     CDS             38172..38798
                     /locus_tag="OO315_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5889716.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38872..41070
                     /gene="zntA"
                     /locus_tag="OO315_000690"
     CDS             38872..41070
                     /gene="zntA"
                     /locus_tag="OO315_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5889717.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41172..41417)
                     /gene="tusA"
                     /locus_tag="OO315_000691"
     CDS             complement(41172..41417)
                     /gene="tusA"
                     /locus_tag="OO315_000691"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5889718.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41638..42303
                     /locus_tag="OO315_000692"
     CDS             41638..42303
                     /locus_tag="OO315_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5889719.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42376..42933
                     /gene="dcrB"
                     /locus_tag="OO315_000693"
     CDS             42376..42933
                     /gene="dcrB"
                     /locus_tag="OO315_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5889720.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42937..44154)
                     /locus_tag="OO315_000694"
     CDS             complement(42937..44154)
                     /locus_tag="OO315_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5889721.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44288..45337
                     /locus_tag="OO315_000695"
     CDS             44288..45337
                     /locus_tag="OO315_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5889722.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45392..45979
                     /gene="acpT"
                     /locus_tag="OO315_000696"
     CDS             45392..45979
                     /gene="acpT"
                     /locus_tag="OO315_000696"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5889723.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46090..47664
                     /gene="nikA"
                     /locus_tag="OO315_000697"
     CDS             46090..47664
                     /gene="nikA"
                     /locus_tag="OO315_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5889724.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47664..48608
                     /gene="nikB"
                     /locus_tag="OO315_000698"
     CDS             47664..48608
                     /gene="nikB"
                     /locus_tag="OO315_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5889725.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48605..49438
                     /gene="nikC"
                     /locus_tag="OO315_000699"
     CDS             48605..49438
                     /gene="nikC"
                     /locus_tag="OO315_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5889726.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49438..50202
                     /gene="nikD"
                     /locus_tag="OO315_000700"
     CDS             49438..50202
                     /gene="nikD"
                     /locus_tag="OO315_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5889727.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50199..51005
                     /gene="nikE"
                     /locus_tag="OO315_000701"
     CDS             50199..51005
                     /gene="nikE"
                     /locus_tag="OO315_000701"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5889728.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51011..51412
                     /gene="nikR"
                     /locus_tag="OO315_000702"
     CDS             51011..51412
                     /gene="nikR"
                     /locus_tag="OO315_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5889729.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51532..51891)
                     /locus_tag="OO315_000703"
     CDS             complement(51532..51891)
                     /locus_tag="OO315_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5889730.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51888..52163)
                     /locus_tag="OO315_000704"
     CDS             complement(51888..52163)
                     /locus_tag="OO315_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5889731.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52222..53346)
                     /locus_tag="OO315_000705"
     CDS             complement(52222..53346)
                     /locus_tag="OO315_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5889732.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53346..56081)
                     /gene="rbbA"
                     /locus_tag="OO315_000706"
     CDS             complement(53346..56081)
                     /gene="rbbA"
                     /locus_tag="OO315_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5889733.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56078..57145)
                     /locus_tag="OO315_000707"
     CDS             complement(56078..57145)
                     /locus_tag="OO315_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5889734.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57511..59133)
                     /locus_tag="OO315_000708"
     CDS             complement(57511..59133)
                     /locus_tag="OO315_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5889735.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59395..60984)
                     /locus_tag="OO315_000709"
     CDS             complement(59395..60984)
                     /locus_tag="OO315_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5889736.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61369..62400
                     /locus_tag="OO315_000710"
                     /pseudo
     CDS             61369..62400
                     /locus_tag="OO315_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62487..62963)
                     /locus_tag="OO315_000711"
     CDS             complement(62487..62963)
                     /locus_tag="OO315_000711"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889737.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62956..63720)
                     /locus_tag="OO315_000712"
     CDS             complement(62956..63720)
                     /locus_tag="OO315_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5889738.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGLAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63966..64727
                     /locus_tag="OO315_000713"
     CDS             63966..64727
                     /locus_tag="OO315_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5889739.1"
                     /translation="MLSKITQELLKMNNVLLMRPDDSEFMIPDDWSGLNAKGITYEQD
                     NRVTESFLPFKSFYCSPVCTTVELISQNNCFAMGIRYLLEKKRDLRLNHFKAFSHKCE
                     TKPDVLIMTGCTVADTYSIFSRIVEENIMKTMTPIVIITGHHSEDLCSLASKLGGVKI
                     ISPTEKIAKIENAINDAFNSKPKKIRFLTDILSPQQIETMKMVAQGRSVNEISAAMNI
                     SIKTVYAHKVRAINKLGIKKKVYEALFYKALIQFN"
     gene            complement(64834..65334)
                     /locus_tag="OO315_000714"
     CDS             complement(64834..65334)
                     /locus_tag="OO315_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889740.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65485..>66630)
                     /locus_tag="OO315_000715"
     CDS             complement(65485..>66630)
                     /locus_tag="OO315_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5889741.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16186 a        17885 c        16347 g        16212 t
ORIGIN      
        1 ccctttggcc ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga
       61 tatgatcagc gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt
      121 tcagcatcgc ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct
      181 gacaatcgac atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca
      241 gcgaatcaac cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga
      301 tttgcgtcag cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc
      361 cctgacgacg cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag
      421 caccgacatg atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac
      481 cgttaagcgc aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct
      541 gttcagacag atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga
      601 tgaacacgac tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt
      661 cattgatcaa atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa
      721 aaatattcac gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg
      781 taatcacatg caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta
      841 catcgaacaa cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca
      901 gacgcttttt gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac
      961 agtgttgttg cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt
     1021 cgtccagcac ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac
     1081 cgttggtgac gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt
     1141 gcaccatcag attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg
     1201 ccagccaggc catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca
     1261 gcagatcggt atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt
     1321 caaagatgat ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt
     1381 ccagcgcctc gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa
     1441 acgcgtcgtc ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg
     1501 acagtaccgg atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg
     1561 ttttatgcaa ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt
     1621 attcctgacg caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc
     1681 gagatacgtt ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga
     1741 atttaccgag gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg
     1801 cgtcggtgtc gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt
     1861 caatccagcg cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc
     1921 gcgtgttgtg gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact
     1981 ctttctggct accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt
     2041 aaccgcgccc cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc
     2101 ccaggccacc gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta
     2161 accccatcgc ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt
     2221 tggagagcgt acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt
     2281 gcgacaactg ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa
     2341 ataacgttgc gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa
     2401 acatcagctt gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat
     2461 atgtaaacgg agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct
     2521 cacggtacga cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca
     2581 caaaccgcca cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca
     2641 gcgaccaggc attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct
     2701 caagagaaca gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg
     2761 taccgtactt caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca
     2821 gaatgacgtc cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa
     2881 cgcccacctg accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat
     2941 cgcccgcgcc gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca
     3001 gcaccaggtc gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt
     3061 tatcgtcaac cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt
     3121 ccaacgtatc gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt
     3181 tctcgtccac gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca
     3241 gaccttccat accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga
     3301 ccgccgtaat gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga
     3361 attccagccc atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg
     3421 cataaaacat gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc
     3481 cgcgcgccgc cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat
     3541 caggacgcca gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg
     3601 caaaacgcaa tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg
     3661 gacgatcata gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga
     3721 acaacagcgt gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca
     3781 cgccacggcg aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga
     3841 tttgtgctgc gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga
     3901 acatctctga acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg
     3961 tagcatttcg cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc
     4021 ttcctctgcg ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac
     4081 gcagcggcgc agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc
     4141 aatgttgcag aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga
     4201 accggagatc acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga
     4261 gcgatcctgc acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt
     4321 gcgatcgtac atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta
     4381 agcttccagc gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca
     4441 agagagcggg aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc
     4501 tttgccaaag tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag
     4561 atagtcggcg tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg
     4621 cattgacggt gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac
     4681 cgccataacg ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca
     4741 acgtgcgcct ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg
     4801 gtcgcccgcc aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg
     4861 ggtgaccgca tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg
     4921 cagcagatcg acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg
     4981 ctgcaccaga gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt
     5041 gatgcagtta gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg
     5101 ttttgctcgc ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag
     5161 ggcaacagat ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact
     5221 aaccatgact aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc
     5281 tgccagacaa ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac
     5341 tccccagcag gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag
     5401 cgatccgaga gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa
     5461 ggttgagcat atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc
     5521 tccaccaaag cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa
     5581 ccactgcttg cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg
     5641 ttattgccat gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg
     5701 gagagcaaca acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca
     5761 agagtaccgc ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg
     5821 cgattttctt ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc
     5881 gtaaaaccgt catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt
     5941 ttaaaaacat cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa
     6001 tgtagccaga aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc
     6061 gggaaatttc ccacctgata accactagga gcgatatccc acggttcagc aattaacttc
     6121 acctgcgaga gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac
     6181 tctggcgtac ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt
     6241 tctacccaat aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg
     6301 agcgtgttgc cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag
     6361 ctacggttat cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta
     6421 tggttgagca cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca
     6481 tcgcgaaact catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac
     6541 atcgccaccg ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca
     6601 ctggcaaact gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag
     6661 ttgatcatca ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc
     6721 gggtgcaagt acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc
     6781 gtgcgcggcg gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc
     6841 gcaatggcgg cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta
     6901 tctttaaact ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta
     6961 aagcgatgcc cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc
     7021 gggcgcgcat ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag
     7081 cgatgttcct ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag
     7141 aaaagtgtga agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca
     7201 atggcgagtt gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg
     7261 tcaggcttag tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc
     7321 cattgcctgc attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt
     7381 tacctggctg attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg
     7441 caacgataat ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt
     7501 tgtcatccac caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt
     7561 tatggtggcg gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt
     7621 tatcgccgcc ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct
     7681 gggcaaattc gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat
     7741 agtaagcgcg caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg
     7801 attttttacc gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt
     7861 tgtagagcat cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct
     7921 tcatgtagtc cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac
     7981 cgcccatatc ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca
     8041 ccgcaccgga aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt
     8101 caagattctc gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt
     8161 cacgataaat catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa
     8221 tccagtaaag cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga
     8281 tcagcgtgtt ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg
     8341 tgccatcaaa ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga
     8401 gaatcacgtt cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag
     8461 taccaaaacg gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga
     8521 agggatgctc gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag
     8581 gcaccagttg atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac
     8641 gccaggaacc caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt
     8701 tgcgctcttc agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg
     8761 ggcgcatttg cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat
     8821 caatcatctc gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga
     8881 tgccgctctc tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc
     8941 caaccaccga gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc
     9001 catccatagt atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag
     9061 ataatagcca ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca
     9121 ggttttgctg accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg
     9181 gaatgacgcc gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc
     9241 cggttttcgg ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca
     9301 gtcccgcggt ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa
     9361 ttagcgcgtt aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt
     9421 gtgtcacccc atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg
     9481 gcgcagcact ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact
     9541 catgtattca gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg
     9601 aaacatctgc gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt
     9661 aatgaataaa gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca
     9721 cagcagctgg tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg
     9781 caggcggcct accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat
     9841 agtgatttcc cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc
     9901 ttcgacggtc ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca
     9961 gcgcaatgcc ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag
    10021 gatcttgttg gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg
    10081 tttgtcgatc cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag
    10141 ctcaccgcta cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg
    10201 ggtcgcgagt tcatctgcca catggccata cagatggccc atctgggtta ataactcacg
    10261 catatgtcgc gcaccaccgc cggaagcggc ctggtaggtt gcaacggaca cccaatcaac
    10321 aagatcattg gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc
    10381 gccaacaaaa gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg
    10441 gtcaagaatg atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta
    10501 accttgccat ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg
    10561 acaggtcaca atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt
    10621 gccagtggtt ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac
    10681 agggcgaatg gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac
    10741 cataccgcgc cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt
    10801 gtgctgtata aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca
    10861 aagtcgcaag tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct
    10921 ttgttagcac gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca
    10981 tttctgcagc agttttattg atcctgatta tggatccgct cggaaacctg cctattttca
    11041 tgtccgtact gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc
    11101 ttattgctct cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc
    11161 tcagcctgcg agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca
    11221 ttaagatgat tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc
    11281 catttatcgt gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga
    11341 tgttgttgtc tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg
    11401 cctggggcgg cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg
    11461 agaaaggggt gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc
    11521 agatgttcct cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa
    11581 agagcccggt agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa
    11641 tcatcccaac gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca
    11701 gcgtttcggc ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa
    11761 cgtggctgac aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac
    11821 cagagtagtt cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta
    11881 cggtggcagc cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa
    11941 tcggcaggcc catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca
    12001 gcacctgttt gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca
    12061 gcgcgtgacc gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc
    12121 cataaatcgc taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga
    12181 accattcgta agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca
    12241 ccagcaccag cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt
    12301 cgccgagatg cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga
    12361 agttacccca cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca
    12421 ggatcatcca gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag
    12481 gcaccaggaa cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca
    12541 ggttcgtacc ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca
    12601 cttcaaagaa cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc
    12661 ggctgtgacc gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat
    12721 ataagatctt gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc
    12781 ctcccatccc tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc
    12841 cagcccccat ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta
    12901 aaaacagcag cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta
    12961 cttatttgcc ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat
    13021 cgatatccac caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact
    13081 gcgtcaccaa catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa
    13141 tcacatcaaa atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca
    13201 agtcgcgata gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct
    13261 gcatagcaaa cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca
    13321 gcggttcgcc agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa
    13381 gaaacgcggc atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg
    13441 atacgcccat caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc
    13501 cacaatacaa gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga
    13561 caatatcaga agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt
    13621 tgtgggctta aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg
    13681 tcacagattc gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct
    13741 cacgcggcgt cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt
    13801 ggaaaccggc aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg
    13861 ccgcgccgac cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc
    13921 gccgcgcctg gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca
    13981 cgctatatgg caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga
    14041 tagtacgttc gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa
    14101 tggcggtagt catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc
    14161 acggcgactg gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta
    14221 aggtgcgcgg cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca
    14281 tggattcgag acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct
    14341 gataaccgtg cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt
    14401 tggtgagaga aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg
    14461 gcgcacgatt gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag
    14521 cgacggaaac ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta
    14581 cacggtcagc cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa
    14641 agagatagat tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc
    14701 ttgtttacaa ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg
    14761 caaaaccccg gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag
    14821 gcaggcttca aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg
    14881 ctgtcgctat ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg
    14941 gtcgaggctt tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg
    15001 cgttcagcgg caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt
    15061 tccatatcct gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt
    15121 ttgccctgca cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca
    15181 ggcatgatcc agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag
    15241 tgacgaatac ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta
    15301 ttgcccatgc tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata
    15361 tctttatgcg ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg
    15421 cgcagcgcgg agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc
    15481 caggagtcca gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc
    15541 atttgtattc gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga
    15601 tgatagaggg tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc
    15661 aaacttaatt cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga
    15721 tttccagatt ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg
    15781 tttgatacaa cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc
    15841 tgaccgtcac accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga
    15901 atcccggttc gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt
    15961 atttaataaa cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca
    16021 gatggctggt tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt
    16081 cgtcaggatt ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac
    16141 cgaacagaga aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct
    16201 gcggcagtcc aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt
    16261 cgacaatctc tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat
    16321 caaagcgacg attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca
    16381 gctctttcgc ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg
    16441 cttccagcgc gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca
    16501 acttaacatc gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt
    16561 aaatgggagc ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc
    16621 tatccttgcg gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa
    16681 taaaggcgca gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc
    16741 gcaaagcgga cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata
    16801 aattgagaac gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag
    16861 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa
    16921 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    16981 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    17041 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    17101 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    17161 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    17221 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    17281 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    17341 atgattgaaa tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc
    17401 gataacgcgc cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt
    17461 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa
    17521 tagagaattt gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat
    17581 attaataccc cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac
    17641 cgtccggccc aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct
    17701 gaccttttgc ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct
    17761 cgtccggcaa ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc
    17821 cataatcgat gctattcacc accatttgca gcaccgtggt gataatccgg ctaccggtaa
    17881 ccagccaggt tttaccgtct ttcaccacaa tggtcggcga catcgacgac agcgggcgtt
    17941 tgttcggccc gacggcgttg gcatcaccgc ccaccagccc gtaaacgttc ggtacgcccg
    18001 gtttggcgga gaaatcatcc atctggttat taagcagaat accgctctca cccgcgacaa
    18061 tacccgtacc gaaggtggtg ttcagcgtat aggtcaccgc caccgcgtta ccgtctttat
    18121 ccactacaga gtaatgggta gtttgattac tctcataagg cgcaagcttg ccagggcgaa
    18181 tctcactgga cggcttcgct tttttgatat caatttgatc ggcaatggat ttggcatagg
    18241 ctttattggt cagtgcctgc cacgggactt tgacaaaatc cgggtcgcca agatattccg
    18301 agcggtcggc gtaggcgtat ttctccgctt ctgccatgat ttgcatcgca tcggcgctgc
    18361 caaagccgta tttcttcata tcgaagtttt ccagaatatt gaggatttgt acgatatgga
    18421 tcccgccgga ggatggcggt ggcatggagt aaacctgata cccgcgataa tcgccgctta
    18481 tcggagtgcg ttcgaccgct ttataggccg ctaaatcttc tttagtgatc aagccaccgt
    18541 ttttctgcat ctcctgggcg atctgttccg caatcgtgcc tttatagaat tcgtccgggc
    18601 cgttttcagc aatcatctcc aggctctttg ccaggttcgc ctgcaccagc gtgtcgccct
    18661 ttttcagcgg ctcgccctct ttccagaaga tagctttact gttttcgtga ttcggcaaca
    18721 cttcgctacc gtaggttttg agatcgtcag ccagcgcgtc gttaacgata aaaccatcgc
    18781 gtgccagttt aaacgcgggc tgcacgactt tgttcagcgg catggtgccg tatttatcca
    18841 gcgccagcga gaaacctgct accgtacccg gtgtgccgga agccagatgc gaagtgagtg
    18901 attttttgct gtccgggttg ccctgatcat cgaggaacat atcgcgggtc gctttggcgg
    18961 gtgccatttc gcggaaatcg atagccgtgg tattgccatt tttcgagcgg attaacataa
    19021 aaccaccacc gcccagattc cctgcctgcg gatgcgttac cgccagcgcg tagcccaccg
    19081 ccacggcggc atcaacggca ttcccgccct ccttgaggat atccaccccc acctgagtgg
    19141 cagtggcgtc cacagacgct accattccct gtttcgcgcg taccggatgg aagacatctt
    19201 cctccacacc atacgagacg ggcggcgcag gaggcgcggc ggcggcgcta aaacaacttc
    19261 ctgagagcag agcagcaatg gccacccggc gtaaaaacgt cggttttatc atcgttattc
    19321 tccagagatt aaggggcaac cccagctaag cctggtatat aactctgaat taatcatcgt
    19381 tttgtcggga agcgagtaaa cttaaaggat atcttcagag gaggatacgc gatgaaacga
    19441 cttctgattc ttacggcact cctgccgttt gtcggctttg cacagcccat taatactctg
    19501 aacaacccta accagccggg gtatcagata cccagccagc agcggatgca aacccagatg
    19561 cagactcagc aaatccagca aaaagggatg ctgaatcagc aactgaaaac gcaaactcag
    19621 ttgcaacagc agcatttaga aaaccagata aacaataatt ctcagcgggt gttgcagtca
    19681 cagccggggg agcgaaatcc cgcccggcag caaatgctgc ccaacaccaa cggcgggatg
    19741 ttaaacagca accgtaatcc ggatagttcg ttgaatcagc agcatatgct gccggagagg
    19801 agaaacggcg acatgctgaa tcagcccagc acgccgcagc ctgacattcc gttgaaaact
    19861 attgggccgt aaagttcgga ccaatcacgt caatcgcatc ggtacagatg caatccacgc
    19921 cccagcgcag caactctgcc gcgtgctggg gtttattgac ggtataaacc agaatccgta
    19981 atccggcgtc tttcaactgc atcactcgcg ctttatcgag taacttatga ttgagatgaa
    20041 tagagacgca gcccagccgc gcggtcagtt cgcgccagtc gtcgcgccac tcatcaagca
    20101 ataaaccacg cggcagttcc ggtgccgcct gttgtgcagc ttctaaagca tcaatctcaa
    20161 acgatgacag cagcggcggc gtcataccgg cccacagttg gcgtgccgcc agcgccacca
    20221 ttttgcccgt taatggtcca gtgccagtgg tgggtttgat ttcgatattc gccatcatcc
    20281 cgtgttcgcg acagcgttcc gccacctgcg aaagcaacgg cagcggctca cctttaaacg
    20341 ctttgctgta ccaactgccc gcatccacgc gcagtaaatc ctgccagttc agttcacccg
    20401 cgacgcccca gccgttgctg gtgcgttcga gattgtcgtc atggagcagg aagatctcgc
    20461 catctttcga taacttcgcg tcaaattcga tcatcttatg accgtatttt gccccgacgt
    20521 cgattgccgc cagggtgttt tccggggcca gcttaccgcc gccacgatga gcgacgatgc
    20581 ggggataagg ccagttactc atactcgttg tcctgtttca ccatcaaaaa gatgcagctg
    20641 attttccgcc agatgcagcc acagcgtgct gcctgccgtc gggcgctcct gatgcgccag
    20701 tcgcaccacc agcttctgct tgcgagctta gcgcaatatg ttccgggcga atgccgaggg
    20761 tcattttacg cccggcatac tgacggtagc caccgtttag cggtagcacg ataccgccgt
    20821 ccagttcgaa atgcgtgcct tcattattca tgcggcctgt cagcaggttc atcgccgggc
    20881 tgccgataaa actcgccaca aacaggctgg cgggcttttc gtagacttcc accggcgtgc
    20941 caatctgttc ggcaacgccg ccgttcatca ccattactcg ctgggcgagc gtcatcgcct
    21001 caacctgatc gtgagtaacg tagagtgaag tcgttttcag gcgacggtgc agctgttgca
    21061 gttcaagacg catctgtacg cgcagcttgg catcgaggtt agagagtggc tcatcaaaca
    21121 agaacaccgc cggatcgcgc acaatcgcgc gtcccatcgc cacacgctgg cgctgaccgc
    21181 cggaaagctc gcgcgggcga cgtttgagca gaccgtccag ctccagaatg cgcgccgctt
    21241 ctttaacgcg ctcggctatt tgctgcttgc ccatgccgcg aattttcagc ccccacgcca
    21301 tgttttcttc gacgctcata tgcggataaa gcgcgtaatt ctggaacacc atcgcaatcc
    21361 cgcgatcttt cggctccatt tcggtcacgc gctggtcgtt gatccagata tcgccttcgg
    21421 tcaccctctc cagcccggca accatacgca gcagcgtcga tttcccgcag ccagacggcc
    21481 cgaccatcac gataaattcg ccatccgcca catcaagggt cagcggttta atgacctagg
    21541 ttttgccatc ccagcttttg gttactgcct gtaatttcag tcctgccatc ttatttctca
    21601 ctatcgacca ggccgcgcac gaaggcacgc tgcatcacta aaacaatcac tactggtggg
    21661 atcagggtta acaacatcgc cgccatcact gagttccatt ccgtggtgcc ttcgccagtg
    21721 gcgatcatcc ctttgatccc tgccacggtg gtgccgagat ccacgtcggt aataatcaac
    21781 aacggccaca aatactggtt ccagccgtaa ataaaggtga tcacaaacag cgccgccaga
    21841 ttagttttgg agagcggaaa aacgatgtcg caaaagaagc gcattggtga cgcaccgtcg
    21901 atccgcgccg cttccaccag ttcatccggc agcgtcataa agaactggcg aaacagaaac
    21961 gtggcggtcg ccgaggccat cagcggcagc gttaaaccgg cgtagctgtc gagcatcttc
    22021 aggttggcga tgacttccac cgtcgggaag atacgtactt caaccggcag catcagggtg
    22081 ataaaaatca tccagaagaa gaggttacgt agcggaaaac gaaaccagac aatggcaaat
    22141 gccgagagca tcgagacagt aattttgccg agcgtaatgc tgaacgccat cacaaagctg
    22201 ttaagcaaca tccgccagaa cggcgcgcta tttgtgccta ccccgttcac ccagatgttg
    22261 tggatgtttt ccagcagatg cgtgccgggg atgagcgtca tcggcgcggc gtagacggcc
    22321 tgtttatcca gcgtcgccgc gacaaacgcc acgtacagcg ggaagaggat caccgcgatc
    22381 ccgaggatca gcatggtatg gctgaatatc gtcagccacg gacggttctc aatcattggt
    22441 aacgcacctt gctttcaaca tagcggaact gcaccaccgt cagcacgatg acgaggaaca
    22501 tcagcaccat cgactgtgcg gcagacgaag ccagatccag tccggtaaaa ccttcgcggt
    22561 agatcttata aatcagcgtc gtggtggcct gaaccggccc gccggacgtg gcggcgtcga
    22621 tcaccgggaa ggtgtcgaag aaggcataca ccaggttcac taccagcagg aagaaactca
    22681 ccggggcgat aagcggcagc gcaatcttaa agaagcggcg aatcggccct gcaccgtcga
    22741 tggctgcggc ttcgatcaac gaacggggaa tggattgcag cgcggcatag aagaacagaa
    22801 agttgtagct gatttgcttc cacaccgagg caaacaccac cagaaacatc gcctgaccgc
    22861 tattttgcgc gtggttccag tcgtagccga actcagcgag aaaatgggtg atcagcccgc
    22921 gaccggggtt aaacaggaag atccacaata cggcggcaac ggcgggagcc acggcatacg
    22981 gcagcaacat taaggtttgg tagaaacgac tgccgcgcac gatgtactcc accagcgccg
    23041 caaagaacag cgataccagc aaaccgctga cggtgacaaa ggtgctgaat tttatcgtcg
    23101 tccagaagga gtcgagataa tagctgtcat gaaacagcgt gacgaagtta tccagaccga
    23161 caaactggct ggagaaacca aacggatcga cgctttgtag cgagtaccac aacgcttcgc
    23221 ccgcaggcca gataaaaaag ataacggtga tgatgagctg cggcgcgacc agcagataag
    23281 gcagccagcg cgagcggaac accggacggg atgatgacat tacggttaat tcctgaactg
    23341 tgccggatgc gcttcgctta tccggcctac acgcgatgcc aggtcagata agcgaaacgc
    23401 atccggcatt acactgatta agacttcgtc gatttctcaa agcggcgcag taactggttc
    23461 ccacgctcaa ctgcggtatc cagcgcctgc tgtggcgtct tcttaccggt ccacacgctc
    23521 tcaagctctt catccacaat cacgcggatc tgcggcatgt tgcccagacg aagccctttg
    23581 gtgaacggca acggcggctt gttcagcatc tgacgcgtcg cggtatccgc acctgggttt
    23641 ttctcgtaaa agccctgctc acgggtcagg tcatacgccg ctttggtaat tggcagataa
    23701 ccggttttct gatgccactc agcagcgttt tctggcttcg cgaggaagtc gagaaacttc
    23761 gccacgccgg tatacgtttc tttatctttg ccctgcatca cccacaggct ggctccgccg
    23821 ataatggcgt tttgtggcgc atctttcgca tcggcgtcgt aaggcatcat acctacgccg
    23881 tagttaaatt tggcgtactc gcgaatgttg gcaagagaac cggaagaggc ggtggtcatc
    23941 gcgcaatcac cgttatagaa cttctcggtg gattcatcct tacgaccgac gtagctgaag
    24001 tcgcccttct tgttcatctc ctcgagcatg gcgatgtgtt tcacctgctc cggcttgttg
    24061 aactccagca ccgcgtccgt gccgtcaaag ccgttgtttt tgctggcaaa cggcagaccg
    24121 ttccaggcgc taaagttttc cagttggatc cagccctgcc agccgctggc gtagccgcac
    24181 ttaataccgg aggctttcag ttttgcggaa tagtccgcca gatcctgcca ggttttcggc
    24241 ggctgttccg ggtctaaccc cgctttcttg aaggcgtctt tgttgtaata gagaacggaa
    24301 gtcgagctgt tgaacggctg ggagagtaag tggccggttt tgctgtcgga gtagtaaccg
    24361 gaaaccgtcg gcacaaactg cgactcatcg aactgaatcc ctgcctcttt aaacacgtca
    24421 tacaccggtt taatggcttt cgacgccatc atggtggcgg tgccaacttc ataaacctgc
    24481 aaaatagccg gagcgttgcc ggtacgaaat acggcaatcc ccgcgcttaa attctgttcg
    24541 tagttgcctt tataggtcgg tacaattttg taatccggat tttcggcgtt aaaacgttgg
    24601 gccagagaat ccacctcttt acccagttcc ccttccatag aatgccagaa cggaatggtc
    24661 gtcactgcct gtgcattccc cattaacgcc agtccgagcg ccagtgctga agctgtataa
    24721 tgtaacggtt tcatcgttta tctctcttgt tgtaccgaat gcgcgaattc acgcgtttta
    24781 tgctcgcggg gtaacatgac atgctcgaat tacagaaaaa taactttttt gttacatttg
    24841 taagatagta aggtgtcaga aagatgacaa ggcggtgacg gcgtgggtga gggaaaatgg
    24901 gagatggggc acggataagc ggaaaaatag aaggtctgaa tcaaactcta cagattactc
    24961 atcgtttcat gccggatgcg gcgtaaacgc cttatccggc ctacaagatc gtgcaaattc
    25021 aatatattgc aactcaccca gtaggcctga taagcgcagc gcatcaggca attttgcatt
    25081 tgtcacctgt ctcaaaggag tcttttgact ccttatcaat caacgtgtta ttacccgcct
    25141 aaatacgcac ttctcactgc ttcgttcgcc agcagcgcgt cgccggtatc ggagagcacc
    25201 acatggccgt tttccagcac gtagccgcga tccgccagct ttagcgcctg gttggcgttc
    25261 tgctcgacga ggaagatagt catcccctgc tcgcgcagct gctcgatggt gtcgaaaatt
    25321 tgctggatga taatcggcgc aagaccgagc gatggctcat caagtaacaa caaacgcgga
    25381 ttgctcatca gcgcgcgacc aatcgccagc atctgctgtt caccgccgga catggtgccc
    25441 gcccgctgaa cacggcgctc atgcagacgt ggaaacagtt catacaccca ctttatgcgc
    25501 tcctgaaact ggtcacgttc ggcaaagaag ccgcccatcg ccaggttctc ttccaccgtc
    25561 atccgcgaga agacgcgacg cccttccggg acaatcgcta ccgcttcgcg catgattttc
    25621 gccgtctgcc agtcggtaat gtctttatca tcaaacacaa ttcgcccgct ggtggcgcgc
    25681 ggatcgccgc ataacgtgcc gagcaaggtg gttttccccg ccccgttcgc gccaatgaga
    25741 gtgacaatct cgccctgatt gatatgcagg ctcacctcat gcagcgcctg gattttgccg
    25801 tagtgggcgc tgactttgtc aaaggacaac atgacttttt ccatcttatg cctcacctaa
    25861 ataggcacgg atcacgtccg ggttattacg gatttgctcc ggcgtaccgt ttgccagtgg
    25921 cgtcccctga ttgaccacat aaattcggtc tgaaatcccc atcaccagct tcatatcgtg
    25981 ttcaatcaac aaaatagtgg tgttgtgatg attgcgcagt tcggcaatca gctcatccag
    26041 ctctttggtt tcttttgggt taagacctgc cgccggttcg tcgagcatta aaatctccgg
    26101 ctgcgtcacc atgcagcggg caatctccag acggcgttgg tcaccatagg ccaggttact
    26161 cgcctgacgg ttggcgtgtt ccagcaaacc aatgcgctca agccaggtgg cggcgcggtc
    26221 gagcgcttcg ctctgggcgc gacggaaaga aggcgttttc aacaggccag agaacagccc
    26281 ggttttcagt tgctgatgct gcgccaccag caggttttca attaccgtca tttcacggaa
    26341 cagacgcaca tgctggaagg tgcgcaccac gcccatgcga gcaatttgct gccccggtaa
    26401 accttcaaga tgctgatcgc gcagtaaaat ggtgccgccg gtgggtttgt agaaaccggt
    26461 caggcagtta aaaaccgtgg tttttccggc accgttaggg ccgattaacg agacgatttc
    26521 ctgcgggtac agttcaagat tgacgttgtt caccgccagc aggccgccga agcgcatcat
    26581 cagaccgtta acagataata atggctgact catgcctgct ctcctttcgc tgcgccgttt
    26641 ttcagcttca gttgcgggcg cgtcatgggc agcaaaccct gcggacgcca gatcatcatc
    26701 agcaccatca aaccaccgag cattaacatg ctgtattcgt tgaaatcacg catcaattcg
    26761 cgcgacacca ccagcagaac agccgccaga atcactgcaa attgcgagcc cataccgccg
    26821 agcaccacta tcgccagcac aaaggccgat tcagcaaagg tgaaggattc cgggctgaca
    26881 aagccctgac gcgccgcaaa cagcgttccg gcaaaaccgg caaatgcggc gctgatggta
    26941 aaagcagtca gcttgatacg gcgcgggctt aagcccagcg aacgacaggc gatctcgtct
    27001 tcacgcaacg cttcccacgc gcggcccagc ggcatccgca gcaggcggtt aatgacaaac
    27061 aggcttaaca ccaccagcag caacgccacc aggtagagga agataacgcg atcggacgga
    27121 tcgtatttca ggccaaagaa attactgaac gtgtcccagc cgccttcacg agcggtacgg
    27181 ctgaactcga gtccgaagag tgtcggtttc gggatctgac tgattccgtt cgggccgccg
    27241 gtgatttcag tgttattgag cagcaatatg cgcacaattt cgccgaaacc aagggtaacg
    27301 atcgccagat agtcaccgcg cagacgcagc accggaaaac cgagcaggaa acccgccgcc
    27361 gctgccatca gtccggcaat cggcaaacag gtccagaacc ccagaccgta atagtgattg
    27421 agcagcgcaa aagtgtaagc gccgatggcg taaaaaccgc cgtaccccag caccagcaga
    27481 ccagaaagac caacaaccac attcagcccg agaccgagga taatgtagat catggtcaga
    27541 gtggcgatat ccaccgtccc acgtgacacc ataaacggcc acgccaccac aagcaccagc
    27601 agcgccacga ggaacagctt ttgcttcacc gtggacccat caatcgcggg cagaataaac
    27661 ttcggcccgg aaacgctttt caaccctttc tggaaagccg gtcgcaaaag ctggaagaaa
    27721 aagaccaccg ccgtgccgat aaacatccac tgccaacgga tgtctgaagc cgtgtcgacc
    27781 accagtttgg tgccatccag ctccagttgt acgcccataa agacgcccgc caggacaaag
    27841 aacatcgcgg cagagagcag tgccattgca atatgcatcg gtttcatact ttctctacct
    27901 ccgggcgacc cagaataccg gtcggcatca ccagcagcac cagaatcagc agggcgaatg
    27961 agaccacatc tttatattcc gtactcagat aggcagaaga gagcgcctcc gcaatcccca
    28021 ggatcagccc gccaatcatc gcccctggaa tgctgccaat cccaccgagc accgcagcgg
    28081 taaaggcttt catcccggcc ataaagccga tataggggtt aatgacgcag tagaactgac
    28141 cgagcagtac gccagccact gccgccatcg ccgcgccaat aacaaaggtc aaagcaatca
    28201 cccggtcggt gttgatgcca agcaggctcg ccattttcag atcttccgcg caggcacgac
    28261 aggcgcgccc catacgggaa tagcgaatga aaatcgtcag cgccagcatg gcgaggaagg
    28321 tgacaatcca gatcaccgcc tgcatggtgg taatagaggc agagaagttt tcgctatggc
    28381 ccaccaccca ctgaccgtta aacaggctcg gcagcgccac gtcgcgcgaa ccttcggtca
    28441 ggctgacgta gttttgcagg aagatggaca taccgattgc ggaaataagc gcaatcaggc
    28501 gcttagagtt acgcaccggg cggtaagcta cccgttcgat actccagccg taggcgctgg
    28561 cgatgacgat tgcgccgacg aatcccgccg ccaccagcag ccagccggta tcaatgccca
    28621 tcatcatcag cgcggcgatg atcataaatg agacgtagct gccaatcata taaacctcgc
    28681 cgtgggcgaa gttgatcatg ccgataatgc cgtaaaccat ggtgtagccg atggctatca
    28741 gcgcgtaggt actgcccagc gtgacgccgt taaacatctg ctgcaagaaa tacaaaaact
    28801 gctcagacat aaggtaacct ttctaaaccc gcccgcattt tacgggcggt gggatgatca
    28861 cttggctgcc gtggatgaac cgtcggcgtg ccactggaag acaccaaaat caaatccctt
    28921 aagatcgcct ttttcatccc agttcagcgg cccaatcacg gtgtttgcac cgttagcttt
    28981 taaatctttc accagcgcca gcggctcatc gctgccggta cgctcaaggg cagttgccag
    29041 agattgcact gccgcgtaag tgatccagac ataaggcccg gacggatctt tcttgtctgc
    29101 tttcagcgca tcaacgatgc cctggtttgc cggatcctgg tcatagcgtt ttggcatagt
    29161 gaccaacatg ccttcggcgg catcaccggc aatgttcgac agcgatgcgt taccgacacc
    29221 ttccggcccc ataaactggg ttttcaggcc aacggaacgg gcttggcgca gcatctgccc
    29281 catttccggg tagtaaccgc cgtagtaaac gaagtcgata ttttcttttt tcaggcgggc
    29341 gatcagcgcg gagaaatctt tctccccggc ggtaataccg tcgaagaaga cgacgttggc
    29401 gttagccgct ttcagcccgt cctgcaccga acgcgccagc ccttcgccat actgttgttt
    29461 gtcgtgaatg atggcgatgc gctggggctt caccgtctca agaatgtatt ttgccgccgt
    29521 aggcccctgg gaagagtcca gcccggcagt acgcataatg tgttgataac cgcgttgggt
    29581 cagctccggg ttggtcgctc ccggcgagat catcagaata ccttcgtctt cgtagatatc
    29641 cgacgctggc tgggtagagg aagaacacag atgaccaata acgtatttaa tgccgtcgtt
    29701 aacgattttg ttggcgaccg caacggcttg tttcgggtcg caggcgtcgt catattccac
    29761 gccaaccagc ttgtcgccct taattccccc tttgacatta atgtctttaa tcgcctgacg
    29821 cgcgccgtta aattccatat cgccccactg ggcaatcggg ccggacatcg cgccgacaac
    29881 ggcgacttta atatcgtcag ccatagcggt gtgtgaaatt gccagtgcaa tcatccctgc
    29941 gatgatagtt ttcgcattcc gtttcatagt caaaaatccc cattcgtgat gttgtgttgc
    30001 tttgttttta tgtgttaaca aatcagactg ttcttttttt atactgcact gtttttgcct
    30061 gtctgattaa ggggttagcg cagtattttg tgatgatagc gattaaaatc cctatttttc
    30121 agtcgattaa gaacagataa tattctgaat ttattgatag ataaacagaa aaaagtgcct
    30181 ttgtcagcat aaaataacgg cacaaagggc ggaataattc actatcattc aggggattat
    30241 gctggacatt tttcattctc taatgtttta actttgtaat tattgctgtt aaaaaattaa
    30301 tcacctgcca aaagaaataa aaaagagaaa gcctccgatt aaattatttc gctacactgg
    30361 ttccactttt gtgatttaca cgggttaccc atgaagctga ccatcattcg attagaaaac
    30421 tttagcgacc aggaccggat tgacctgcaa aagatctggc cggagtattc cccttcctct
    30481 ttacaggttg acgataacca ccgtatctac gccgcgcgtt ttaacgagcg tctgcttgcg
    30541 gccgtgcggg taaccttaag cggcaccgag ggagcactgg attctctacg cgtgcgggaa
    30601 gtcacccgcc gtcgcggtgt ggggcaatat ctgctggaag aggttttgcg taacaatcct
    30661 ggcgtttcat gctggtggat ggcggatgca ggcgtggaag atcgcggtgt gatgacggcg
    30721 tttatgcagg cactgggatt tacggcacag cagggcggct gggagaagcg ttaatcgtca
    30781 agttgatttc aaaagtgatg accaacgaga aattgcctga tgcgctacgc ttatcaggcc
    30841 tataatgtgt gctgcaattt actgatttct ttggttcttg taggccggat aaggcgttta
    30901 cgccgcatcc ggcatgaagc aacgtattcg atattagcaa tttggcggca acccaaagtt
    30961 gccgattaac aattacttcg catcggtcgc cgtgccgttg gcgtgccagt caaatacgcc
    31021 gaactcaaag ccctttagat cgcctttctc atcccaggtc agtggcccca tcacggtatc
    31081 cacggagttc gcttttaggt atttggcgat ttcagccgga tcgtcagact gattcaggcc
    31141 cgcctgcaaa gattgcagcg cggcgtaggt ggtccagacg aatgcgccgc ttgggtcctg
    31201 ttttttcgct ttgatcgcat caacaatggg tttgttcgcc ggaacctgat cgtagttctt
    31261 cggcttggtc accagcagcc cttccgctga ttcgcccgca atgttagaca gcgaaacgtt
    31321 agccacacct tccggcccca taaactgagt tttcagccct gccgcgcgtg cctgacgcag
    31381 gatttgcccc atttccgggt gataaccgcc gtagtaaacg aagtcgatat tctctttttt
    31441 cagacgcgcc accagcgttg agaaatcttt ttccccggcg gtgataccat caaagaacac
    31501 cacgttagca ttgcctttct tcaggccgtc ctgcaccgct cgtgccagac cttcgccgta
    31561 ttgctgtttg tcgtgaacga tagcaatacg ctgcggtttc actttctcaa gaatatattt
    31621 cgccgccgtc ggcccctggt cggagtccag gccggtggtg cgcaggatca gctgatagcc
    31681 acgggcggtc agctccggcg cggttgccgc tggggtgatc attaaaatgc cttcgtcttc
    31741 gtagatgtca gacgcaggct gcgttgatga agaacagagg tgaccaatca catatttaat
    31801 gccgtcgtta acgactttgt tcgccaccgc aaccgcctgt ttcgggtcac aggcatcgtc
    31861 atattttacg atttgcagtt tgttgccttt aatgccgcct ttagcgttga tatccgcaac
    31921 cgcctgctct gcgccggtaa actcctggtc accgtactgc gcaaccggac cggacattgc
    31981 acccacgacc gcgactttaa tatcttctgc cagagccata ttgctgaatg ccagcgcgat
    32041 acatcctgcc agtaacgctt taccctttat gttcatcctg agaatcccca ttcttctggt
    32101 tattacgtgt gttgtgatgt tgttgttcag cactttattt cgttttatgc atgactaccc
    32161 gtgctttagc agcatactct gctaaaacat acccgatttt tatgatattg gaatagctat
    32221 tttgacagtt tattaacaat ctgcgtgggg attggcgttt tgccggaggg ggaattgatt
    32281 attgtagagg cccaaaaaac aaaaaccccg gactctcatc cagggttctc tgcttaatag
    32341 cggaaattac gcttcaatag cggcgcgaag ttttttcatc gcgttctttt ccagttggcg
    32401 cacacgttca gcggaaacac cgtaacggtc agccagttcc tgcaacgtgg acttgttgtc
    32461 ttcgtccagc cagcgcgcac ggatgatgtc ctggctgcgt tcgtccagac catgcatcgc
    32521 gtcggtcaga cggtttgccg cctgctcttc ccagttatca tcttcgatgc cgtcggcaaa
    32581 gttagatgat ttatcctgca gatagagcac cggtgccatc ggctggctgt cggaatcgtc
    32641 gtcggaagac aggtcaaagg tcatgtcctg tgccgccata cgtgattcca tctcacgtac
    32701 gtctttgctg gttacgccca gttcacgggc caccatttcg acttcatcct ggttaaacca
    32761 gcccagacgc tgcttggttt tacgcaggtt gaagaacagt ttgcgctgcg ctttggtggt
    32821 cgcaactttg acgatacgcc agttacgcag aacgtattcg tggatctctg ctttgatcca
    32881 gtggacggcg aaggagacca ggcgcacacc cacttccggg ttaaaacggc gcactgcttt
    32941 catcaggccg atgttacctt cctgaatcaa atccgcctgt ggcaggccat agcccgcata
    33001 attacgagca atatgaacaa caaaccgcag gtgagacagg atcagcgttt tagctgcttc
    33061 cagatcgcca tggtaatgca gcttttcagc cagcgcccgc tcctcgtcag ccgacaacat
    33121 cggccacgcg ttagctgccc ggatgtagga atccaggttg ccaactgggg ctaaagctaa
    33181 actttgcatt ttgtcagtca ttcaaatcct ctcaatcgat atcttctggc gcttcagtgg
    33241 tagcaacaac tgtgccaaag cttaagagca acgaggttat cattcactgt tttatcagac
    33301 cgtgatttta tccacaagtt caatgcaagc ttgtgaataa attacgcaca aaatgtgaca
    33361 tagagatgaa ataccgggaa gagacaacgg ggtctctttc cctgctacgg aacccattgc
    33421 agggaaagag tataacacgc ttttattatt caggcgtaaa gtggcgtaaa tgttgtaccg
    33481 tggcaagcca cgctgccacc cagccaatca tcgagcatac cagcagcaat agcaggcatt
    33541 catcgaacga taaaccattg atatcaaact tcgttccgaa aacctgtgcc acttccgcca
    33601 ccgctgacga caaacgcagt accagaattt ctgacaaaat taatgacaac aatgcgccag
    33661 aaaatcccag cagcgcaccg ccgtacagga acgggcgaag gatgaaacca tccgtcgcac
    33721 caatcagttt ctgcacgttg atggagtcac ggcgagcaaa gatgctcaga cgtacgctgt
    33781 tgccgatgac gaggaacact gccgccacca tcaacacgcc gatcatcgcc gaaacgcgcc
    33841 caaccagccc ggtcaacgcc gccagacggg caaaccagct gtcatccatc cgcacttcgt
    33901 caataccgtt aatctgcgtg atacgatcac gcagcgtatt gagtgattcc gtcccctgaa
    33961 aatcgagttt cgggatcacc accgccactg ccggaagcgg gttttcttcc agcatatcca
    34021 gcgcaccacc aaaaccagac cagttacgga actcgcccag tgcgtcttca cgagaaagat
    34081 agttcacttt ctccacgcct tgctcggcct gcaactgtgc caccacgccc gcagcggcgt
    34141 catcgtccag cgttttttgc aagtaaacag tgatttgcgg tgacggataa tactgcgtcg
    34201 ccgcctgatt aacgtttttg tacaccatat aacagacgct gggcagcgtc agggagatgg
    34261 cgataaccat caccgttaaa aacgtggcga acggtttgct tttcagatcc tgcaatgcgc
    34321 cgtggaaggc atagcgcacc tgttcgttga aaacgttggt tttgcgattt accggttttg
    34381 gcgaggattt cgcgcgtttc ggtgcgttac gaccgccgtc gcctgagccg ccgaccgatt
    34441 tacggaagcg atccagacgc ccaccaaact gccgaatatg attgattgca tcgcgcttat
    34501 tcatggccca cgcctccatg caagtgacca tcgctcaggg tgagcatgcg ataggaacgc
    34561 cgtgaaatca gattaatgtc gtgcgttgcc atcaatacgg ttaccccaac gcggttaaac
    34621 tcttcaaaca gacgtagaat cccttccgac aacgcgtcgt ccaggttacc ggtcggttcg
    34681 tccgccagca gtaccgcggg cttattcacc accgcgcggg caatgccaac acgctgttgt
    34741 tcaccgccgg aaagctgaat agggaagttc ttcgctttgt ccagtagccc gactttatcc
    34801 agtgccgccg acacccggcg acgaatatcg tcaccgctgg caccagcgat aatcagcggg
    34861 atcgccacgt tatcgtagac agtacggtcc atcagtagat ggtgatcctg gaaaatcatg
    34921 ccaatctggc ggcgcagaaa cggaacttca cggtttttca gacgcgtgat gtcatggccg
    34981 ctaaaccaga ttttcccggc gctgggccgc tcaatcccac agatcagctt caggagggta
    35041 cttttccctg cgccggaatg accggtcaga aacgccatct cacccggctg catatggaac
    35101 gtaacgccct gcagcgcctg tctcccaccg agataagcct tgctgacatg ttcaaagcga
    35161 atcattgtta atcctctcgg gcaaaaagtg cctctataaa gtcgtccgcc ttaaacggac
    35221 gcaaatcctc aatacgttcg ccgacaccaa tgtagcggat agggatacca aactggtcag
    35281 ccaccgagaa aattaccccg cctttcgccg tgccgtccag tttcgttagc gtgatgccgg
    35341 ttaagccaac agcttcgtgg aacagtttgg cctggcttac cgcgttctgc ccggtgctgg
    35401 catcaatagt cagcataact tcatgcggcg cttcaacgtc gagtttcttc atcacgcgga
    35461 cgattttctt caactcttcc atcagatgcg atttgttctg cagacgtccg gcggtatcgg
    35521 caatcaggac gtcgatatta cgcgctttag ctgcctgaat ggcgtcgaag ataacagagg
    35581 cggaatccgc accggtatgc tgggcaatca ccggaatatt gttgcgctga ccccagacct
    35641 gaagctgttc aaccgcagct gcacggaaag tatcgcccgc cgccagcatc accgatttac
    35701 cctgctgctc aaactgacgc gccagcttac caatcgtcgt ggttttaccc acaccgttga
    35761 cgcccaccat caggatcacg aacggcgttt tatcttcaac attcagcggc tcatcgactt
    35821 tcgccagaat ctcgcccatc tcttctttca gcaggccata gagcgcctcg gcatcacgaa
    35881 gctgcttgcg ggatgcgcct tccgtcagat tggtgataat tttacgtgtg gtttccacgc
    35941 ccacatcggc gatcaacagc tgttcttcca gctcttcaaa cagatcatcg tcgatttttt
    36001 taccgcggaa caggctgata aatccggaac cgagattttc tttggttttt aacaggctgc
    36061 gtttcaggcg cgcgaagaag ccttctttgg tcggtttttc ttgctcctgg gcaatttctg
    36121 caaccggctg ctcttcttct gccggaggaa ctaccatcac cgcctcttct gccgcttcgg
    36181 cagccagcgc ctgtgcttcc agctcttcgt cggtgatttc ttctttagcc gcttcttctt
    36241 ctgccgcttc gacaatctct acggtttccg cttcagcctg ccactcttct ggcgaaacct
    36301 cttcggcgtt gacgtcttcc ggcaacggca gctcttcacg ttcgatagcc actggctccg
    36361 gcgtttcttc tacgaccggt tccggctgtg caacaacttc cgcttcaggc tgcgcttttt
    36421 cactttcaac aacctgttca gtgacttcca caacgtcggc agcaaaagtt tccgcctcgg
    36481 cttcagtatg cgcctgcggc tgctcttcaa cggcttgttc agaagccttc gcaggctctt
    36541 gcgcctgaac gatttcttct acaaccggtt gttcattctg aacttctgtc tctttttccg
    36601 gggtctgctc tttttgacca aagcccagcc aggaaaaaaa gccacgtctt ttttctttcg
    36661 ccatttgcga ctacactcct cgctgttgct tcatggcaca gcgttaacgc tatgtacata
    36721 gcagctaaaa aaatgatgaa atagtctatc acttaactta attcacatca ccgcctgcaa
    36781 gtatgtgtta tctggcggat tgagcaattt atcatgaaaa aaccgaatca ttccggcagc
    36841 ggccaaatcc gcattattgg cgggcagtgg cgaggccgta aactcccggt tcctgatagc
    36901 ccaggtctgc gccccaccac cgaccgcgta cgcgaaacgt tgtttaactg gctggctccg
    36961 gtcattgttg acgcccaatg tctggattgc ttcgccggga gcggcgcgct ggggctggaa
    37021 gcgttatcgc gctacgctgc gggggcaacg ttgattgaga tggatcgcgt ggtttctcag
    37081 cagttaatta agaatctggc gacactaaaa gcaggcaatg cacgcgtggt gaacagcaac
    37141 gcaatgtcat tcctggcgca aaaaggcaca ccgcataata tcgtgtttgt cgatccaccg
    37201 ttccgccgtg gcttgttaga agagacgata aatttactgg aagataacgg ctggctggct
    37261 gacgaagccc tgatttatgt cgaaagcgaa gtcgaaaacg gtctgcccac tgttccggca
    37321 aactggtcat tacatcggga aaaagtggcg ggtcaggtgg cttatcggct gtatcaacac
    37381 gaagcacaag gagaaagtga tgctgattaa tattggtcgt ttgttaatgc tctgcgtttg
    37441 gggattttta atcctcaatc tggtgcatcc cttcccgcgc ccgctaaata ttttcgttaa
    37501 cgtggcgctg atttttaccg tgctgatgca tggtatgcag ctggcgctat tgaaatccac
    37561 tttaccgaaa gatggcccgc agatgaccac cgccgaaaag gtgcggattt tccttttcgg
    37621 cgtgtttgaa ctgctggtct ggcagaagaa atttaaagtt aaaaaataac cttattgttc
    37681 gccaacaaag ctaacaaagc gcgttccttt atagctcagc gtacctttat cgcccactgt
    37741 cagggcgtgg tactgctggg cgtcgaggcg aaacgtctgc tccattcctc cgctttgcgg
    37801 tttgaagctt gcctcatagc gcatactggt gcctgccgga gtcacttcct gctggcgcga
    37861 acggcgatcg ttaatcggtt tttcccgctt gttgctcacc accaccagct tttgctggag
    37921 cggagccata tcattatcag ctttttcccg tcgctgttgc ataaaacgaa acgatgcggc
    37981 gacgacaatt aagccaatga taacaataaa gaaaagaggt ggtttactca tctttatccc
    38041 tcatcggaaa atgcggaatt aagcataccc tgccagttat ggtgttgtca tccgtccacc
    38101 ctcgccacta aactggaagc aagaccgtag gcattctgct tacgaaaaaa taacgaattc
    38161 aaggaactaa gatgctttgg tcgtttatcg ctgtctgtct ttccgcatgg ctatctgtgg
    38221 atgcatcgta tcgtgggcca acctggcaac gctgggtgtt taaaccgtta acccttcttc
    38281 tcctgctgtt actggcctgg caagcaccga tgttcgacgc cattagctat ctggtgctgg
    38341 cgggactgtg cgcctcactg ctgggcgatg cgctaaccct gttgccacgt caacgtctga
    38401 tgtacgccat cggcgcgttt ttcctctcgc acctgctgta caccatctat ttcgccagtc
    38461 agatgacgct ctctttcttc tggcctctgc cactggtgtt gctggttctg ggtgcgctgc
    38521 tactggcgat tatctggacg cggctggaag agtaccgttg gcctatctgc acgtttatcg
    38581 gcatgacgct ggtgatggtg tggctggcag gtgaactgtg gttcttccgt ccgaccgctc
    38641 cggcgctctc tgcgtttgtt ggcgcttcgt tgctgtttat cagtaacttt gtctggctgg
    38701 ggagccacta tcgccgacgc ttccgtgcgg ataacgcgat tgctgcggcc tgctactttg
    38761 ccggtcactt cctcatcgtc cgctcactgt atctctgata aaacttgact ctggagtcga
    38821 ctccagagtg tatccttcgg ttaatgagaa aaaacttaac cggaggatgc catgtcgaca
    38881 cctgacaatc acggcaagaa agcccctcaa tttgctgcgt tcaaaccgct aaccacggta
    38941 cagaacgcca acgactgttg ctgcgacggc gcatgctcca gctcgccaac gctctctgaa
    39001 aacgtctccg gcacccgcta tagctggaaa gtcagcggca tggactgcgc cgcctgtgcg
    39061 cgcaaggtag aaaatgccgt gcgccagctt gcaggcgtga atcaggtgca ggtgttgttc
    39121 gccaccgaaa aactggtggt tgatgccgac aatgacatcc gtgcccaggt tgaatctgcg
    39181 gtgcaaaaag cgggctattc cctgcgcgat gagcaggccg ccgacgagcc acaagcatcg
    39241 cgcctgaaag agaatctgcc gctgattacg cttatcgtga tgatggcaat cagctggggt
    39301 ctggagcagt tcaatcatcc gttcgggcaa ctggcgttta tcgcgaccac gctagttggg
    39361 ctgtacccga ttgctcgtca ggcattacgg ctgatcaaat ccggcagcta cttcgccatt
    39421 gaaaccttaa tgagcgtagc cgctattggt gcactgttta ttggcgcaac ggctgaagct
    39481 gcgatggtgt tgctgctgtt tttgattggt gaacgactgg aaggctgggc cgccagccgc
    39541 gcgcgtcagg gcgttagcgc gttaatggcg ctgaaaccag aaaccgccac gcgcctgcgt
    39601 aacggtgagc gggaagaggt ggcgattaac agcctgcggc ctggcgatgt gattgaagtc
    39661 gccgcaggtg ggcgtttgcc tgccgacggt aaactgctct caccgtttgc cagttttgat
    39721 gaaagcgccc taaccggaga atccattccg gtggagcgcg cgacgggcga taaagttcct
    39781 gcaggagcca ccagcgtaga ccgtctggta acgctggaag tgctgtcaga accgggtgcc
    39841 agcgccattg accggattct gaaactgatc gaagaagccg aagagcgccg cgcccccatt
    39901 gagcggttta tcgaccgttt cagccgtatt tatacgccag cgattatggc cgtcgccctg
    39961 ctggtaacgc tagtgccgcc gctgctgttt gccgccagct ggcaggagtg gatttataaa
    40021 ggtctgacgc tgctgctgat tggttgcccg tgtgcgttag ttatctcaac gcctgcggcg
    40081 ataacctccg ggctggcagc agcagcgcgt cgtggggcgt taattaaagg cggtgcggcg
    40141 ctggaacaac taggtcgtgt tacccaagtg gcgtttgata aaaccggtac gctgaccgtc
    40201 ggtaaaccgc gcgttaccgc gattcatccg gcaacgggta ttagtgaatc tgaactgctg
    40261 acactggcgg cggcggtcga gcaaggcgcg acgcatccac tggcgcaagc catcgtacgc
    40321 gaagcacagg ttgctgaact cgccattccc accgccgaat cacagcgggc actggtcggg
    40381 tctggcattg aagcgcaggt taatggtgag cgcgtgttga tatgcgctgc cgggaaacat
    40441 cccgctgatg catttgctgg tttgattaat gaactggaaa gcgccgggca aacggttgtg
    40501 ctggtagtac gtaatgatga cgtgctgggt atcattgcat tgcaggatac cctgcgcgcc
    40561 gacgctacaa ctgccatcag tgaactgaac gcgctggggg ttaaaggagt gatcctcacc
    40621 ggcgataatc cacgcgcagc ggcggcaatt gccggggagc tggggctgga gtttaaagcg
    40681 ggcctattgc cggaagataa agtcaaagcg gtgaccaagc tgaatcaaca tgcgcccctg
    40741 gcgatggtcg gtgatggtat taacgacgcg ccagcgatga aagctgccgc catcgggatt
    40801 gctatgggta gcggcacaga cgtggcgctg gaaaccgccg acgcagcatt aacccataac
    40861 cacctgcgcg gcctggtgca aatgattgaa ctggcacgcg ccactcacgc caatatccgc
    40921 cagaacatca ccattgcgct gggactgaaa gggatcttcc tcgtcaccac gcttttaggg
    40981 atgaccgggt tgtggctggc agtgctggca gatacgggtg cgacggtgct ggtgacggcg
    41041 aatgcgttaa ggttgttgcg caggagataa ggcaaaccga tcgcaacatt gagcgcgatc
    41101 ggtcccctcg cccctctggg gagagggtta gggggtgagg aaaaggcggc atcgaagcca
    41161 atcagcccct atcaaccgcc tttacgaatc aaataacgat aaggcagtcc atccgtctct
    41221 ttcgcaacca gttcgtgttc cataaaggta caaaacccag gaatatcgcg ggtagtggcc
    41281 ggatcgtcgg cgataatcag caacgtttcg ccaggctgca tattgcgcac ggttttgcgc
    41341 accatcatca ccggttccgg gcagcgcagg ccaagcgcgt cgagtgtgtg gtcagggctg
    41401 gaaaagagat cggtcatttt cttctcatca cttaaaaaaa cggcgctagt ttacgccctg
    41461 tgagtccgta agcaaaccag gttaacgatt gcgtgaaaat taaccattgc attgtcaacg
    41521 taaagcagta tcatgcggcg gctcgaaaaa agggtaagca cgttattatg ttaaggtaac
    41581 agacgtgtcg tacgtattgg gttccctcac cccaatggtt aatcaaaaag gtacaatatg
    41641 aacgttttct cgcaaactca acgctataag gcgttgttct ggttatcgtt atttcatctt
    41701 ctggtgatca cctccagtaa ctatctggtt cagcttcccg tctccatttt tggtttccat
    41761 accacctggg gcgcgtttag ctttccgttt atttttcttg ctaccgacct gaccgtgcgt
    41821 atttttggcg caccgctggc ccgacgcatt atcttcgcgg taatgatccc tgcgttatta
    41881 atctcctacg tcatctcgtc gctattctat atgggttcct ggcagggatt cggcgcactc
    41941 gcccacttca acctgtttgt cgcccgtatc gccaccgcca gtttcatggc ctacgcgctg
    42001 gggcaaatcc tcgacgtgca cgtttttaac cgcctgcgtc agagtcgccg ctggtggctg
    42061 gcaccgacag cgtccacact gttcggtaac gtcagcgaca cgctggcctt tttcttcatt
    42121 gccttctggc gtagcccgga tgcctttatg gctgaacact ggatggaaat cgcgctggtc
    42181 gattactgtt tcaaagtgtt aatcagtatc gttttcttcc tgccaatgta tggcgtatta
    42241 ctcaatatgc tgttgaaaag actggcagat aaatccgaaa tcaacgcttt gcaggcgagt
    42301 taaaggttcg ttatcagagt tgtgataaga tggatgaatg agccgttatg gccgtttatc
    42361 gaaaggaaga agtcaatgcg caatctggtt aaatatgtcg gaattggcct gctggttatg
    42421 gggcttgcgg cctgtgatga taaagacact aacgctacgg cgcagggttc ggttgcggaa
    42481 agtaacgcta ccgggaatcc cgtcaacctg cttgatggca agttaagttt ctcgctgcca
    42541 gcggatatga ccgaccagag cggtaagctg ggaacgcagg ccaataacat gcatgtctgg
    42601 tccgacgcca ccgggcagaa agcagtcatc gtcatcatgg gcgatgatcc gaaagaagat
    42661 ctggcggtgt tggcgaagcg tctggaagat cagcaacgta gccgcgatcc gcagctgcaa
    42721 gtggtaacca ataaagccat tgagctgaaa ggtcacaaaa tgcagcagtt agacagtatt
    42781 atctccgcga aaggccagac ggcgtactct tccgttattc tgggtaacgt gggtaatcaa
    42841 ctgctgacca tgcaaattac gctgccagct gacgatcagc aaaaagcgca aaccaccgca
    42901 gaaaacatca ttaatacgct ggttattcag taagttttaa gatgatgagg cggcctcagg
    42961 gacgtgttcc ggaggccgtt tttttaatcg ccacgtcagt aataacgcga ttgcgaccag
    43021 tcccgccgcc gccagataaa tcaccggtac gcccgcccag ctcatcacca gcccagccag
    43081 tggtccagtc acgccaagcg ataaatccat aaatacggtg taagttgcca gcgccgcccc
    43141 ctgattttgc tgcggaaccg cttttaccgc cactacaccc aatgccggga acaccagcga
    43201 aaacccggcc cccgccagta agacgccgat tttcgccatc cacggcatag tcgccacgcc
    43261 aaccagtagc aggccgatta tctcaacgct aaagcaaatc atcgctacgt ttaagccacc
    43321 gatacggtta atgccgttag ggaataacaa acgcgtaccg acaaacgcac agctaaacag
    43381 cgtcagcgcg aaagccgcac cgtcccaacc tttagcgtca taaaacagcg tgataaaggt
    43441 ggcgatgacg ccaaatccgg cggaagccag tgccagcgcc ataccgtaca gccagacgcg
    43501 cccaagcacc gcgcgaaacg gcagcggttt gcctttactg gcttttaccg tcggacgcgg
    43561 gatcgccaac aaaatggcca ccagcgccac gcccataatg attaacgcta acgcctgcaa
    43621 gccgccccag tgataaaaca cgacgcctaa cggcgcaccc atcgccatcg ccccgtaagt
    43681 gacaatgccg ttccacgaaa tcacccgccc gatatgcagc gagccaacca ctccaacgcc
    43741 ccatagggtc gatcccgttc cggcaaaact ttgcccaatc ccaaggatga cgcgccccag
    43801 gcaaagtaat aacaggctga tgacaggcag actggcggtt aatcctgccg tcagataccc
    43861 cagaccgctc aaaaagcagc cgcataaacc gaagacgaca atctttttgg gtcccagcaa
    43921 atcggcgtaa cgtccggcat gagggcggct cagcaaggtg gcgaaatatt gcaggctgat
    43981 aaccaatcct gcccagaagg cgctaaagcc catcacatca tggacatagc ccggtaatac
    44041 agcgagcggc aacccgatgg tgaggtagct ggcgaagtta aacatgacaa tggagacaat
    44101 gcgcaaattc aggcgcaatc cgtttagcgc gggttcggct acgggttcgg gcatgaggat
    44161 caccacattt ttacaacagt gtttcatttt taccatgtgc tgacgtgaaa atcagcagta
    44221 agaatcagaa tattgctggc gtgactcccg cgctacactt aacacaaaaa gtcacaagga
    44281 agccccaatg gaaacccctc aacccgataa aacgggcatg cacattctgc tcaagttggc
    44341 ctcgctggta gtgatcctcg cgggcattca cgcagcggca gatatcattg tgcagctgtt
    44401 actggcgctg ttttttgcca tcgtcctcaa cccgctcgtt acctggttta ttcgtcgggg
    44461 agtacaacgc cccgttgcca ttacgattgt agtggtggtg atgctgatcg cactaaccgc
    44521 gctggtcggc gtactggcgg catcgtttaa cgaatttatc tctatgctgc cgaagtttaa
    44581 taaggagctg acgcgcaaac tttttaaatt gcaggagatg ttgccttttc ttaatttgca
    44641 tatgtcgccg gagcgaatgc tgcagcggat ggactcggaa aaagtggtta ccttcaccac
    44701 agcgctaatg accgggcttt ccggggcaat ggcgagcgtg cttttgctgg tgatgaccgt
    44761 agtttttatg ctgtttgaag tgcgccacgt cccttacaaa atgcgttttg cgctgaataa
    44821 cccacagatt cacatcgcag gattacaccg cgcacttaaa ggcgtttcgc attatctggc
    44881 gttaaagacg ctactcagtt tatggacagg tgtcatcgtc tggctggggc tggcgctaat
    44941 gggcgtacag tttgcgctga tgtgggcagt actggcgttt ttgctcaact acgtgcccaa
    45001 tatcggcgcg gtaatttccg ccgtaccgcc aatgattcag gtgctgctgt ttaatggtgt
    45061 ttacgaatgt attctggtcg gcgcattgtt tttagtggtc catatggtca tcggcaatat
    45121 tttagaacca cggatgatgg gccatcgcct ggggatgtcc accatggtgg tatttctttc
    45181 attgttaatc tggggatggc tgctcggccc ggtagggatg ctactttcgg tgccattaac
    45241 cagcgtgtgt aaaatctgga tggaaacgac gaaaggtggc agcaaactgg cgattttact
    45301 ggggccgggc agaccgaaaa gtcggttacc gggatgaggc gacaagtgat acgatacgca
    45361 ctttcatttt ccattaaacg ttggccctga tatgtatcgg atagttctgg ggaaagtttc
    45421 gaccttaagc gcagctccac tgccaccggg tttacgcgag caagcaccgc aaggtccacg
    45481 acgcgaacgc tggctggcgg ggcgtacatt gctttcgcac acgctttccc cgctaccgga
    45541 gatcatctat ggcgaacaag gcaaacctgc attcgcgccg gaaacgccgc tatggttcaa
    45601 cttaagccat agcggtgacg atatcgctct gctgttgagc gatgaaggcg aagtcggctg
    45661 cgatatcgaa gtgattcgcc cgcgcgtcaa ctggcgctgg ctggcgaacg ccgtgttcag
    45721 cctcggggaa cacgctgaaa tggacgccgt gcatcctgat cagcaactgg aaatgttctg
    45781 gcgcatctgg acgcgcaaag aagccatcgt taaacagcgt ggcggcagcg cctggcaaat
    45841 cgtcagcgta gatagcacct atcactcctc gctgtcagtc agccattgtc agctagaaaa
    45901 tttaagcctt gcgatctgca cccccacccc ctttacgctc accgccgaca gtgtgcaatg
    45961 gatcgattca gttaactgat ccgcccaccc gactgcccat ctattgatcc agaacaggta
    46021 atcagtatga cgaatactta aaatcgtcat acttatttcc gccatctatt ttaatccatt
    46081 ggggttacca tgctctccac actccgccgc actctatttg cgctgctggc ttgtgcgtct
    46141 tttatcgtcc atgccgctgc accagatgaa atcaccaccg cctggccggt gaatgtcggg
    46201 ccactaaacc cgcaccttta cacgcctaac cagatgttcg cccagagcat ggtttatgaa
    46261 ccattggtga aatatcaggc agacggttcg gtgatcccgt ggctggcaaa aagctggact
    46321 cattcagaag atggtaaaac ctggaccttc accctgcgtg atgacgtgaa attctccaac
    46381 ggtgaaccgt tcgatgccga ggcggcggca gaaaacttcc gcgcagtgct cgataaccgt
    46441 caacgtcacg cctggctgga gctggcaaac cagattgttg atgttaaagc actcaataaa
    46501 acagagctgc aaattaccct gaaaagcgcc tactatcctt tcctgcaaga actggccctg
    46561 ccccgtcctt tccgttttat cgctccctcg cagtttaaaa accatgaaac catgaacgga
    46621 attaaagcgc cgattggcac cggaccgtgg attttgcagg aatcgaaact gaatcagtac
    46681 gatgtcttcg tccgtaacga aaactactgg ggcgaaaagc cagcgattaa aaagatcacc
    46741 tttaacgtca tcccggaccc gactacccgc gcggtggcgt ttgaaactgg cgatatcgac
    46801 ctgctgtacg gaaacgaagg gttattaccg ctcgatacct tcgcccgctt tagccagaat
    46861 ccggcttacc acacccaact gtcacagccg atcgaaaccg tgatgctggc gctcaatacc
    46921 gccaaagccc ccaccaacga gctggcagta cgtgaagctc ttaattacgc ggtaaacaaa
    46981 aaatcgctga ttgataacgc gttatatggc acccagcagg tcgccgacac cctgtttgcc
    47041 ccttctgtgc cttacgctaa tctcggcctg aaaccgcgcc agtacgatcc gcagaaagcg
    47101 aaagagttgc tggaaaaagc cggttggacg ctgcctgcgg gcaaagacat ccgcgagaaa
    47161 aatggtcagc cgctgcgcat tgaactgtcg ttcatcggca ccgatgcgtt aagcaaatcg
    47221 atggcggaaa tcattcaggc tgatatgcgc cagattggcg cagatgtctc gctgattggc
    47281 gaagaagaga gcagtatcta tgctcgtcag cgcgacggtc gttttggcat gattttccac
    47341 cgcacctggg gcgcgccata cgatccacac gccttcctga gttcaatgcg cgtaccgtcg
    47401 cacgctgact tccaggcaca gcaaggatta gccgacaagc cgctgattga taaagagatc
    47461 ggcgaagtgc tggcgaccca tgacgaaacg caacgtcagg cgctgtatcg cgacattctg
    47521 acccgtctgc atgacgaggc ggtttatctg cctatcagct acatctcgat gatggtggta
    47581 tcaaaaccgg agctgggtaa tatcccctac gcgccgatcg ccaccgaaat tccgttcgaa
    47641 cagattaaac cggtgaaacc ttaatgttgc gttacgtatt acgccgcttt ctgctgctga
    47701 tcccgatggt gctcgccgcc tcggtgatca tttttctgat gctgcgcctc ggtaccggcg
    47761 acccggcgct cgattatctg cgtttatcta acctgccgcc gacgccggag atgctggcct
    47821 ctacccgcac catgctcggg ctggatcagc cgctgtacgt ccagtacggt acctggttgt
    47881 ggaaggcgct gcatcttgac tttggtatct cattcgccag ccaacgcccg gtactggacg
    47941 atatgctgaa cttcctgccc gccacgctgg aacttgcagg tgcggcgctg gtattaattc
    48001 tgctcacttc cgtaccgctc ggtatctggg cggcgcgcca ccgcgatcgt ttgccggatt
    48061 tcgccgtgcg ctttatcgcg tttcttggcg tgtcgatgcc caacttctgg ctggcatttt
    48121 tgctagtgat ggcgttttcg gtgtatctgc aatggctacc cgcgatgggc tacggcagct
    48181 ggcagcacat cattttacct gcggtttcca tcgcctttat gtcgctggcg attaacgcgc
    48241 gactgctgcg cgccagtatg ctggacgtcg ccggtcagcg tcacgtcacc tgggcgcggc
    48301 tacgcggcct gaatgacaaa cagaccgaac gccgccatat tctgcgcaat gcctcgctgc
    48361 cgatgatcac cgccgtgggg atgcacattg gcgaattgat tggcgggacg atgattatcg
    48421 aaaacatctt tgcctggcct ggcgtcgggc gctatgcggt gtcggcgatt tttaaccgtg
    48481 actatccggt gattcagtgc tttacgctga tgatggtggt ggtttttgtg gtctgtaatt
    48541 tgattgtcga tttgctcaac gccgcgctgg acccgcgcat tcgtcgtcat gaaggagcgc
    48601 acgcgtgaac tttttcctct cttcccgctg gtcggtacgc ctggcgctga tcattatcgc
    48661 cctgctggcg ctgattgcgc tcaccagcca gtggtggcag ccgtacgatc cacaggcaat
    48721 tgatttgccg tcgtgcctgc tttcgccgga tgcgcagcac tggctgggca ccgatcactt
    48781 aggtcgcgat attttctcgc ggctgatggc agcgacccgc gtgtcgctcg gttcggtaat
    48841 ggcctgcctg ctgctggtgc tgacattagg gctggttatt ggcggcagcg ccgggttgat
    48901 tggcgggcac gttgatcagg ccaccatgcg cgtcgccgat atgtttatga ccttcccgac
    48961 ctcgattctg tcgttcttta tggttggcgt gctcggcacc gggctgacca acgtaattat
    49021 cgccatcgcc ctgtcgcact gggcgtggta tgcacgcatg gtgcgcagct tggtgatttc
    49081 actacgccaa cgcgagtttg tgctggcgtc acggctttcc ggtgcgggcc atgtgcgggt
    49141 gtttgtcgat catctggcag gcgcggtgat cccttcgctg ctggtgctgg caacgctgga
    49201 tatcggccat atgatgctgc acgtcgcggg gatgtctttc cttggcctcg gtgtgaccgc
    49261 gccgaccgcc gaatggggcg tgatgattaa cgacgcgcgc cagtatatct ggacccagcc
    49321 gctgcaaatg ttctggccgg ggctggcgct gtttatcagc gtgatggcct ttaacctggt
    49381 gggtgacgca ctgcgcgatc atctggatcc tcatctggtg acggagcacg cacactaatg
    49441 ccgcaacaga ttgaactacg taatatcacg ctacaggccg cgcagccgct ggtacacggt
    49501 gtatcgttaa ccctgcaacg cgggcgcgtg ctggcgttag tcggcggtag cggcagcggg
    49561 aaatcattaa cctgcgccgc gacgctgggc attttgcccg ctggcgttcg ccagacggcg
    49621 ggggaaattt tagccgatgg caaaccggtt tcgccttacg ccctgcgcgg catcaaaatt
    49681 gccaccatca tgcagaaccc gcgcagcgcc tttaatccac tgcacaccat gcacacccac
    49741 gcgcgggaaa cctgcctggc gttagggaaa cccgccgatg acgctacgct taccgctgcc
    49801 atagaagcgg tggggctgga aaacgccgcg cgcgtgctga agctgtaccc gttcgagatg
    49861 agcggcggca tgttgcagcg catgatgatt gcgatggcgg tgctgtgtga atcaccgttt
    49921 atcatcgccg atgaaccgac caccgacctc gacgtggtag cacaggcgcg catcctcgat
    49981 ctgctggaaa gcattatgca aaaacaagcg ccgggaatgc tgctggtcac ccatgatatg
    50041 ggcgttgtgg cgcgtctggc ggatgacgtg gcggtgatgt cacaaggtaa aattgtcgaa
    50101 cagggcgatg tagaaacgct gtttaacgcc cccaaacata cggtgacgcg cagcctggtt
    50161 tccgctcatc tcgccctcta cggtatggag ctggcatcat gaccttactt aacgtctgcg
    50221 gcctttccca tcactatgcc cacggtggat ttagcggaaa acatcaacat caggcggtgc
    50281 tgaataacgt ttccctgacc ctgaaaagcg gcgaaaccgt cgccctgctg ggacgcagcg
    50341 gctgcggaaa aagcaccctc gcgcggctgc tggtaggctt agagtctccc tcacaaggga
    50401 atattagctg gcacggcgaa tcgctggcga aacttaatcg cgcccaacgt aaagccttcc
    50461 gccgtgatat tcagatggtg tttcaggact ccatcagtgc cgtgaatccg cgcaaaaccg
    50521 tgcgcgagat cctgcgtgaa ccgatgcgcc acctgctatc actgaaaaaa tccgaacaac
    50581 tggcgcgcgc cagcgaaatg ctgaaggcgg tcgatctcga tgacagcgtt ctcgacaaac
    50641 gcccaccgca gttaagcggc ggccagctcc agctcgtctg cctggcacgc gcgctggcgg
    50701 tcgaaccgaa attgctgatt ctggatgaag ccgtttctaa ccttgatctc gtgttacagg
    50761 cgggcgtcat tcgcctgctg aaaaagctac aacaacagtt tggcactgcc tgcctgttca
    50821 tcactcatga cttacgcctg gtggaacgct tttgccagcg agtgatggtg atggacaacg
    50881 gacaaatcgc cgaaacccag gcggtgggag ataaattaac cttttcctct gacgccggac
    50941 gtgtgctaca aaacgcggta ttacccgcat tccccgtgcg ccgtcgcacc tcagaaaagg
    51001 tttaacgcca atgcaacgag tcaccatcac gcttgatgac gatttactgg agacgctgga
    51061 cagcctgagc cagcgtcgtg gttataacaa ccgttccgaa gctatccgcg atattctgcg
    51121 tagtgccctg gcgcaggaag ctacccagca gcacggcaca caaggtttcg cggtgctgtc
    51181 gtatgtgtat gaacacgaaa aacgcgactt agccagccgt attgtttcca cccagcatca
    51241 tcaccacgac ctctccgtcg ccacgctaca tgtgcacatc aaccacgacg actgtctgga
    51301 aatcgccgtg ttgaaaggtg acatgggtga cgtgcagcat tttgccgatg acgttatcgc
    51361 ccagcgcggc gtgcggcacg ggcatttgca gtgcctgccg aaggaagatt gagttatttc
    51421 taaaacaagg aaagaaaaga tattaactga acttaattta taaaattaag ttcagttgca
    51481 tagatagatc ctgtaaaggc agatattctg cgtaatgtac gtcgctaaaa tttataaatg
    51541 attcaaacgc tcattcagtg tttcaataat ataggtatta acagagactt gttgctgcgc
    51601 agccgcatta ttaaggcgtt cactcagcga ttctggatag cgtaacgtaa aggtcttaat
    51661 cttttctgta cgcgcatacg gctcaatacc cgcagcttta caatcctcaa gatactctcg
    51721 cagggaaagc tctccttctt tttgcagccc ctgaatactg tcagagacaa aatcacaata
    51781 acctgacaag cccaggaatt tgccacgaaa tgcattcagt tctggcacgt aggtaataac
    51841 agctggttgc ccggcaatct ccatagagtt tggcgtcttc agtttgatca tggttttacc
    51901 cctatactta gtaaccagtc acacacgctt tctacagcgc ctttatcggt atctggcgac
    51961 ggatgaggcc gatggaaaca cgcaacgctc atatttagta tgaacttgca acgcgaacct
    52021 cttccttcct taatttctcc gcctaatgct ttaaccagtg actctatatc agaccacttg
    52081 atcccttgag gaacaggagt tttaaatatt tgatccaatg tatttttttg tttcttccgt
    52141 aaagcctgta ccttggttct catggcatgt ccttatgact tcactttatg acatcacagt
    52201 atgaagtcaa gtttcccttc cttacgccat tgtcccaatc gtcttcctga atcgcagcag
    52261 tgcgatggtg aaaaacgcgc cgccaattgc catcagcgtc aggaactgcg gccagacgat
    52321 ttcgaatccg gcaccccggt agaggatggc ctgcgcgagg ctaacaaagt gtgtcgtcgg
    52381 catggtcagc ataatgtcct gcaccatctg cggcatactt tcgcgcggcg tggatccacc
    52441 ggaaagcatt tgcagcggca gcagcaccag aatcaccagc agccccagtt gcggcattga
    52501 acgcgctatc gtccccataa aaatgccgat tgacgtggtg gcaaacagac tgagcgccac
    52561 gcccagcata aacagcggga tcgagccttc aatcggtacg cccagtacac ctttcaccat
    52621 cagcaccagc gataatcccg ataccaccag caccaccagc cccatcgacc agatcttcgc
    52681 catcatgatc tcaaacggcg ttatcggcat caccagtaaa tgttccaccg tgccgtgttc
    52741 acgctcgcgg atcagcgccg atccggtcaa tacaatcgcc agcatggtaa tgttgttgat
    52801 gatcgccatc accccgccaa accacgcggg atcaaggttc gggttaaagc gcatccgggt
    52861 ttccagcgat accaacggtt cgctgttatc acggtagcgc gcgacaaagc tgttcacttc
    52921 accgttgata atattctgga tatacccatt gccggtaaat gcctggctca tgcgcgtggc
    52981 atcgacgttc acctgaatat ccggctggcg tccggcgagg acatcacgct gaaaattagg
    53041 cggaatattt atcgcgaagg tatagcgtcc ggcgtccagt ccggcatcca tctcatcggc
    53101 ggtgatcatc tccggtggca aaaaccacgg acgatagaag ctgttaacga tccggttcga
    53161 taactgcgat tgatccatat cggcaatggc gatcggcgcg aggttcaacg atcctggcgt
    53221 aacggtcgct gacgaataca ccgacaccgt aaacgagaag acaatcagcg tcagcatcgc
    53281 tttatcaccg agcagactgc gcaactcttt gatacccaga ttaaaaatat tgcgtaaatg
    53341 gcgcatcatc cctcctgttt tttcagcagc aggatgctta agcccatcac cagcgggatg
    53401 gctatcagca acgggataaa aagttgccac aaatcagtca gatccagcgc tttcgagaac
    53461 gttccgcggg cgatagtcag aaaatgactg gtcgggtaaa cctcgccgat ccaacgtcca
    53521 ggcccttcca gcgaagctac cggatcgatc atcccggaaa actgtgtcgc cgggatcaat
    53581 gtgataatcg ccgttccgaa aatggcggca atctggcttt tcataaaggt ggagatcagc
    53641 agccccattc cagtggcaat gatgatatac agcagcgccg ccaaggtgag cgtcaggaaa
    53701 ctgcctttat gcggtacgcc aaacacaaac accgacaggc cgcagagcag gaaaaagttc
    53761 agcatcccca gcgcgatgta cggcagctgt ttgccaagca aaaattcgct acgggtggtg
    53821 ggggtcacgt aaaggttgat aatcgaccct agctcttttt cccgcaccac gctaagggcg
    53881 cttagcattg acgggatcat catcagcaga agcgggatca ccgccggaac aatcgctggc
    53941 aggcttttta cgtccgggtt atagcgataa cgcgtctcaa tgttcatcag cccgctttgg
    54001 ctggcgggtg tcgattgtcg gctcgccaca tcctgaagcc aactctggtg cattgcctgc
    54061 acgtaacctt ttaccgtttc ggcacgactg ggcatcgctc cgtcgatcca gacgccgagt
    54121 tccacaggcg taccacgcgc gatatcgcgc ccgaaattgg gcgggatctc aatggccacc
    54181 gtgatatcgc ccgcacgcat ccgacgatca agctcgtcat aactggtgag cggcggctgt
    54241 tcgataaagt aacgggaacc ggagaggttg agtgtccacg cctggctact gacggtctgg
    54301 tcgcggtcga gcaccgcaaa gcgcaggttt tccacatcca tactgatgcc gtaacccatt
    54361 atcagcatca ggatcaccgt tcccatcagc gccagcgtcg aacgtactgg atcgcgtcgc
    54421 agttccagcg cttcgcggcg gctgtagcta aacagacggc gcaggctaaa tccctgacgc
    54481 ggcgcgtggg tggtgtcgtg taccacgggc ggcgcttcgg cttcgttgct ctgccctgcc
    54541 gcttcctgca aataggcgat aaatgcctct tccagactgg cggctccgcg tttctcaacc
    54601 agttcctgcg gtgtaccgct ggcaagcact tttccggcgt gcatcagtga gatgcggtcg
    54661 caacgttccg cttcgttcat aaagtgggtg gagatgaaga tagtcacttt gtcctggcgc
    54721 gagagatcga ccatcaactg ccagaacata tccctcgcca ccggatcgac accagaagta
    54781 ggctcatcga ggattaacat ctccgggcga tgaatcaccg ccaccgccag cgaaagccgc
    54841 tggcgaatgc cgagcggcaa tgactccggc agaacatctt caacgtcgtt gagcttaaaa
    54901 cgctcgctca tttcagccac tcgcgcggga atttccgctt ccgggatgtg aaacaaacgg
    54961 gcatgtaact ccaggttttg ccgcacggtg agttcgttat agagcgaaaa cgcctgcgac
    55021 atatagccca cacgacggcg ggtatcgata tcttttggat caaccggttg cccaaatagc
    55081 cacgcctcac cttcgctggc gggcagcagt ccggtaagca ttttcatggt ggtggattta
    55141 ccgcagccgt tcgaaccaag gaatccaaaa atctccccgc gtggaatgcg gaaattgacg
    55201 tgatcaacgg caacgaagga accaaaacgc atggtcagat cgcgcgcttc gatggcaatc
    55261 tctgcgtttt caggttgata cggtgggatc actaccgcct gatgcgcctg gcgttgcgct
    55321 tgcggtaaca gattaataaa tgcttcttcc agcgtggcgc tttgcgtttg ctgccgcagc
    55381 tcttcggcgc tgccggttgc cagcacttct ccggcattca tcgctaccag ccagtcgaag
    55441 cgttcggcct cttccatata ggcggtggcg accagcacgc tcatattgct ctgccgctgg
    55501 cgaatactgt cgatcagatc ccagaactgg gcgcgggaga gcgggtcaac ccccgttgtt
    55561 ggctcatcaa ggatcaacag ttccgggtcg tggattaacg cgcagcacag cccaagtttt
    55621 tgcttcatcc cgccggagag tttacctgcc ggacgatcgc gaaacggtgc taacccggtg
    55681 ctggtcagca gctcattaat tcgcgcttcc cgctccgctt tatcgtgacc gaacaggcga
    55741 gcgaaaaaat cgacattttc atacaccgac aaggtgtggt agaggttttt gcccagcccc
    55801 tgcggcatcc aggcgatgcg cgggcagacg tctcggcgat gcttcgggtc gtgcatatcg
    55861 ccacccagca ccaccacatt gccctgctca atgacgcggg caccggaaat cagtgacaat
    55921 aaactcgact tcccgacgcc atccggacca atcagcccga ccatgcaacg ggccggaata
    55981 tcgagagtga tattgttcag cgcaacggtt tttccataat gctggctcac gcccgccagt
    56041 tgcgcgacag gcgggacggg aaccagttcc agatgcgtca ttgcggcaac ctcaccacga
    56101 ggtcgtcagg ccacggaagt tcttcattca cccgcaccca cgctacgccc ggtaaaccgg
    56161 ttttgacata ttccagatgc tgctggagta attccggtgg gatacgcgct ttgacgcgga
    56221 acatcagttt cagccgttca tcgctggttt cgacggtttt tggcgtgaac tgggcgacac
    56281 tggcgacaaa actgatggtt gcaggaatac gcagatctgg cgcggcatcg aggatcagcc
    56341 gggcttcacc gcccagtttc agcgtgcccg cctgttcggt tggcaggaag aaggtcatat
    56401 agacgtcgct gagatcgacc atattcagca cccgaccgcc tgccgccagc acttcccctg
    56461 gctcggcaac ccgatactgc acgcgtccgt cacgcggggc tttcagttca ctgtcatcga
    56521 tatctgcggc aatgcgccgt tcagtggctt gcgctgcttc gacccgagtt tgcgcctgaa
    56581 tgatattggt gcgtgctgct tctatagccg ctttagaagc cgaaacctgg gctttcgctg
    56641 attccagcgc agctcgggcg ctctcagcgg cggcgcggtc gtcatccagc tgttgcgcag
    56701 aaatagcccc tcgttgggcg agcgagcggg aacgcgtatg acgtttggca acagagtcca
    56761 gttcggcctg gcgttgattg accagcgact gtgcggcacg agtttcactt tgtcgttgct
    56821 ccagcaaagc ctgcgcggcg gcaacggcgc tttgtgcctc tttgatttgc gcgatggctt
    56881 ccagtcgctg ttcctgcaac acgcgagtat ccatcttcgc cagcacttcg ccttcgcgaa
    56941 caaactggcc ttctttcacc agaatggtgt cgatacgccc ggcaattttg ctggcgatat
    57001 ccacttccgt cgcttcaatg cgcccattgc tgacagcaaa gccttccggc acacccgccg
    57061 ggcgcaacag ccaccacgcc acgacggcag ccaccgccag tgccccgaca acccaccacg
    57121 ccagatggcg cttactctta tccataatcg acccgccata atccctgtaa gcaaacgacg
    57181 cgtgttacaa ctgccgccga acgacgttca gcggattctt ccatccggga taagaaggaa
    57241 acgacaaata cgaccagcgc gcagagcatt gagcgccaac ggggaaggct tagtgaaaac
    57301 gggtagtaat gttgtcatca tcagcattcc tggccgtaaa tgaaacacgc atactcataa
    57361 gtgtgatcta taaaactggc tgacaaaacg gtatccatcc tgttggatcg catttcacgc
    57421 ctgttcagtt gcaggaacag agcattcctt tttacgtttt atcaacagtc tcaaccagat
    57481 agtaacatta atattcatgt tgtctatggt tcagagctgt aacattgtgc gccttctaat
    57541 caaaggcata agtccatttc tgtttttttc atgctggtaa gacttccagg gagtggttat
    57601 ctctggccct gcaccagggt taatttggca attatgcatg ctgaacattc ttccagcatg
    57661 tttactgtca cgggcgttaa gacctaaaac tgtccccgtt tcacctatgc gttctctccc
    57721 accatttccg ttatgtgcaa caacttgatt tatacctaac atttgtgaac atgctaatag
    57781 tgcttcaagt ttaggtctgt aatctgtcga aacagaaaaa taacgattaa aggatcgaca
    57841 agagatagcc cggctagttt taaacttttt gccattaatt atagcatgat gctttttatt
    57901 aataatttcc gctaattcga ccggatcgag agcattggtc atttcatcaa tagagacagt
    57961 tatcggtcct ataatgtaac gttttcgctg atcatcaaaa gttatcccat gatgatttgc
    58021 cattattttg tatttggggt catagaaaca tacaggataa tcttttatcc cgtcgtaggt
    58081 atcctcatca agtggtttca tgcgcgtgaa gttttgcata tagttaccat tgcaattcgt
    58141 ttcatgattt ccagctaata catgaacatt ttcattgata ctcattatgc gttttaacaa
    58201 atcgatgata aattgatcgc cacagatatt actaaatcga tcgccggttt ggtcgccaag
    58261 aaacgtacat ggcgtgactg cgtctttatt aataaccaaa taaggctgaa gactttctaa
    58321 aacattatct cttctgttca gtatactgat tattgtatta acattagtgt caagttgctc
    58381 attaaagttc tcccttagca atacgttgag aacttcatct tctatattaa gcaactggct
    58441 aaggaaattt tctccttcag ccatgattcc caaatgtcct gttgcaaaaa gcgcgtacaa
    58501 aactgcaccg actgagccat cggtatcgcc aaaataggtc ggcctggtac ttgtgggatc
    58561 aacaggtgaa caattggaga aataggttct gtcagcgaac tggggtttta taattgtttc
    58621 gaatttaata tcaaatacat tgtattgcga tatttcccag ttccagatgg gtttgtacga
    58681 ctcagtaact gcaacatcat tagcattatc atttactgtc atattattaa gtaaataaac
    58741 aatgctgcac caattaatat cggtattatc aagagggcaa ccgtaatttt tactattaat
    58801 tgtcacaata aactgataac agttattcct acgtagaata tgagcgcgca tgcccgatag
    58861 catactatat ttttctgaat gattcgtttg tatggtaaat gaaattttgt catcttgaat
    58921 agattgttgg aaacgttcta tttgccaatc atcaattttt tccacaactt caccgagcat
    58981 tgtcctggca atacttgata tatcatcacc tataccattt gcaatatacc caaacagttc
    59041 tttagtgaag ttggtaacat gctcttgcac catatcattt gttgcgattg tcgttgtact
    59101 gccattagtg cctgccacag ttccaatttt catcttttac ctcatattat tcaccgcgag
    59161 gaatacatag ttaacatttt atcttatgac atacacctca tattcgatgg ggaaataatt
    59221 atattttgca caaatcattc agccttaaag cataatatga aatttcttat ctcatagcag
    59281 agaaagtccc taaatattgt agaggcgtca tcagtacata cctctacaat cgattaagaa
    59341 aacctatgaa atactggcaa aattaaaaag agataccaca gcactcatga agttttattg
    59401 aggttgaaaa caactaagcc ctgggacacc ttttttatca ccacgttcgt taggtgctat
    59461 cttccaagaa tttagcccca tggtataatt atcaattttt tgatgctggt ctacccctaa
    59521 tccataatgg ccaaatttct gtttagtttt tttgagcgtc tgtccacatt tgaagatatc
    59581 ttcaggtttt ggtctgaaag cagtagtgtt attaaaataa ttttcatata caggtgtggg
    59641 tgctatagca ttgcttctaa ttagatggag aagatgatca tccatgtgtt gtttatgttt
    59701 tttattacat atatttgcta attcctcagg atcaattggg tctttcatct gattaaatgg
    59761 aacctgcaaa gcaccgagac aataacattg attttcttca tttcttatta ttccgtgatg
    59821 actggttaaa actttagttt ctgagttata attacaaaca tcgagcgttt taattaagtt
    59881 atacgtatca ccggcagaga gtttataatt ttctagtcgt gcagtatagt taccattaaa
    59941 attgatttca tgatttccag ccagaacgat gacattttta ttaatacggc tatctttatt
    60001 accctccata tttcgcatag aatttagcaa agtaaggata tatttatcac caaaaatagt
    60061 actaaatcta tcgcctgtat gatccccaag gaagatacaa ggggttatcg catcgtcctt
    60121 taaaaaaaca tgttgatgca actcattcaa tatcatgtct ctcgtattta ataaattgta
    60181 gagcaattta ttatttttat gtatgtatgc agatgaagcc atgtcttcgt gttttaataa
    60241 ttcacataat aaactccaac ccttttccct gatgcctata tgccctgaaa cgagcaatga
    60301 ataaagtaca gcgcctactc ttccatctgt atcgccaaaa taagccgtgt tggcattttg
    60361 tgggttaatc acaccacatt gtttataaaa gtctatttta gaaaacacag atttaacata
    60421 gctatcaaac ttatccttag gaaacgtctc tttagagaca tcccaacgcc agtcagcttt
    60481 aaaattaccg ctaagaagag taaagaaatt ttcaggtatg atagaaagcc cattaacttt
    60541 ataaattatg tctgatttat ctccggagaa attaataaaa taataaccat ccttacactc
    60601 aatcttaact gtaatgggag acgataattt tatcacatgg ttattgtctg gtataattgt
    60661 aaagataaac gtgttagtag tattatcggc aaaactatta tattcttgaa tatctataga
    60721 tggctcacta ttgtaaaacc atattttctc catctgaaga ctatgttcat ttaccctcca
    60781 gtgaccatgt tgcaggaaat agatgatatg catgccaaaa tcgttatcta tttgcgaatc
    60841 attatccagc ttatcaattt caactatctt tgaacgatgt gttttatcaa ccttgctatg
    60901 gtgttgatga ttattaactt tatctgaatg cttatcttta tggtttgaag ggatgtactt
    60961 aaatatattg tcaattgctt tcataaataa cccgcgatat ttttgacctg ttagtaaaaa
    61021 ataaatgata catgtctgaa agacatattt acgacgatat aaaacaataa tacttatatg
    61081 ttcgctgatt ttaagcaccg agactatccg gtcttttact aaataatgga aaacaatcca
    61141 tccctggaag acaattgtaa aaaaaaacaa cagaataaga attaatctta ggataaatat
    61201 ttatttatca tggccttttg accgctaaaa aatggcactc ggcacagtaa accccgctat
    61261 agttatgcta tgcatttgtc tttaatgata tctcgtcgtt atattaacag gctgaaatta
    61321 tcattaatgc acatttcaat attagcaggg atacctgagg agtttaaagt gaacatatat
    61381 atcgggtagc ttttcaaatt aatccctttg attataggct taatttgcat cgcgttaggt
    61441 ggctttgtgc tggaaagttc agggcaacgt cacgggtcat gtgctgattt ctctggcggc
    61501 catatgcctg gcattattca ctaccgcatt tattatcatt tcgcaactca cgcgcggcgt
    61561 taatacgttt tacaatacat tgttccccat tattggctat gcggggtcaa ttatcaccat
    61621 gatatggggt tgggcactgt tagcaggtaa tgatgtgatg gcagacgagt ttgttgccgg
    61681 ccatgttatt tttggcgttg gtatgattgc cgcatgtgta tcgacggtgg cagcgtcgtc
    61741 cggtcacttt ctgctcattc ccaaaaatgc agcggggagc aagagcgacg gaacaccggt
    61801 acaggcttat tcttcattaa tcggtaattg cctcattgcc gttcccgttt tactcaccct
    61861 gctcggtttc atttggtcta ttacgctgtt acatagtgcc gacataactc cgcattatgt
    61921 cgccttgggt taaccgcaat ctgtgcctgt ctaattggcc tagtggccac aatcgtccat
    61981 caaacacgta atacgttctc atccaaagag cactggctgt ggtgttattg ggttattttt
    62041 ctcggctcag tcacggtgct gcaggggata tacgtcttag tcagttccga tgcaagcgcc
    62101 cgactggctc ccggcattat tctcatttgc ctcggaatga tctgttacag catattctca
    62161 aaagtctggc tactggcact ggtctggaga cgtacctgtt cgttagccaa cagaataccg
    62221 atgataccgg tatttacctg tctgttttgc ttgtttttgt catcattcct cgcggaaatc
    62281 gcacaaactg agctggacta ctttattcca tctcgggttc tggttggatt aggcgcagta
    62341 tgttttacat tgttctctat tgtttcaata ttagaggccg gttctgcgaa aaaaaattaa
    62401 aatttacata atcccagcgt tattcatatc ttaacgctgg ttttttacga acttagtgaa
    62461 ataatgtaga atataaaatt ataattttat attggagcac atgtgttaat gatcaacccc
    62521 gggatactaa aaatatagac attattaaac ttgcttagcc tcgcattgcc tttacctgtt
    62581 aaagatgcat cgagaaaatt gagtctgaag ttctcatcct ttatctgatt ttccagtccc
    62641 gaaactttgg ccttagcatt acctgagatc cgtccgttac tatctatttt gtaggtaaaa
    62701 aaatacgaat gggaaaattt cctcggtgta tcttcatcag ttaagccaac aatcgtaaaa
    62761 ctaccgctac catctttttt taaaatgact gaaaggttag cagagtagta taaagaatga
    62821 tctgttttag catttcttgt taacgtcgac gtacattcaa gagacgttgt gctaccatca
    62881 cgagccttcc agagagcaaa aaaacacacc tgtagcaatg tgaaaacaag tattaatata
    62941 aaatacattt tcttattagt catataatag cacctctgaa caaattgcat aagtttcgtc
    63001 attagaggat tttatacatc ttgataaata ccgctgccct tccccaagca aataatgcat
    63061 actatttaca tcatatataa aatttgcacc tggcacacag ggtaaatgct ttttcgaaat
    63121 ttcagcggcc tctatttgag atttagcgag gccaatctcg ttaagattct ctgaaagata
    63181 taaatcacag gtgtcaatct tacctgattt aaccaatgga taatccgtta tactgtaggt
    63241 atctttaaaa gccacggcaa acatttcaat aataacaatt actaccacaa gcgaaatcaa
    63301 aatgcgaaaa agaggaatac tccttttgcg ctctactttt tcaacaacat cttcttgtaa
    63361 atgagaatca tcataagatt gtttacttat tgttatgttt tcactgatgt aaaagcctac
    63421 tttgggtatc gtaagaatca cattttcttc aacaccaata tttttaaaac ttttgcgtaa
    63481 cagggatata tattggttca atgtgttgtt tgacagatca aatgaaaatc tctccaaaac
    63541 gctatcaatg attgagttcc tgtcaaccgc tccaccatta ctttccagta acactgccaa
    63601 aatacacccc gccgtagctg gaaaatttat ctcatcctgg gtatttacat tcgagagcga
    63661 gcatttgttc ggatcaaaaa taatcgcgtc attgataatg aaaaaacatc tccgctccat
    63721 gcaggcaaca acacaaaata agattttgtc attttagcct attcctggtg gaactgacac
    63781 aagaaactaa aatacaaata taaccctgaa aaagtcatat aaaacttata tccacagaat
    63841 aaaaaatcaa acatgtcagt tttatcaata taaagtatcg caaggcgaaa agtgatgcaa
    63901 gaatactcct aagtaaatga aagcgcacca catgaaatta tcaacacatg taatttcatc
    63961 attaggtgct atcgaaaata actcaggagc tacttaagat gaataatgtc ttactcatga
    64021 gacccgatga ctcagaattt atgatacctg atgactggtc agggctaaac gcaaaaggca
    64081 ttacatacga acaagataat agagtgacag agtcttttct gccttttaaa tctttttatt
    64141 gttcaccggt gtgcacgaca gttgaactca tctcacaaaa taattgtttt gctatgggta
    64201 tccgctacct tctggaaaag aaaagagact taaggctaaa tcactttaaa gccttctctc
    64261 ataaatgcga gacaaaacct gacgtgttaa ttatgaccgg gtgcaccgtc gctgatactt
    64321 atagtatatt ctcacgcatt gttgaagaaa atataatgaa aacgatgaca cctatcgtca
    64381 tcattactgg gcatcactca gaagatcttt gttctctggc atcgaaactg ggaggagtta
    64441 aaataatatc accgacagaa aagattgcaa aaatagaaaa cgctatcaat gatgctttta
    64501 acagcaagcc taaaaaaata agatttttaa cagatatact ctctcctcaa caaattgaga
    64561 ccatgaaaat ggttgcgcag ggtagatccg ttaatgagat ttcagccgca atgaatatta
    64621 gcatcaagac cgtgtatgca cataaagttc gtgctatcaa taagctggga attaagaaaa
    64681 aagtttatga agcgcttttt tataaagcgc taattcagtt caactgatgt aaaaatcacc
    64741 ctcgcttaat aaataatata ttctatgtct ttttttataa gttaaaaaag gcgggatatc
    64801 aaatcccgcc atgaaattaa ctaatcatct gcattattct gcgtagatat aagcctgggt
    64861 acggatggtg taagagtctg gctcaacaac ctgttctttc atatgggaaa cgacgcgata
    64921 ggcaccacct gttccagtat ttttcagcat catataacgc ttaccgccaa tctcttcgga
    64981 ggcagctgaa ggaggatcct gctcaagcgt tgtcagggaa actttcagtt tatggtccgg
    65041 gttattgtca ccagaaatta ctgcatccgc tgaagtactg ctctccgcat aaatatcatc
    65101 cgggaaagtt acagcatagt cgtggctctg cccatcagat gcgccaacaa tacctgttgc
    65161 caaaggcgtc catgcagtta acgtaccgct atatacccct ttcactggtt gaatggtatt
    65221 attaacaacg ataggcttgg tcaaagtaag attagctgac gcttcagtac cctggcttac
    65281 taaagttccc gcctgagaag caaaagctgt ccccgccagc atgattgcta acaatgtttt
    65341 cttcattata ttttctccat gattttcatt tacactgcaa aatgcagttt ctttctttgc
    65401 atttacgtgc tatcgcgaaa atgcttccac caaataatgg aaacatcttc acaaaaataa
    65461 attatgagtt tcttaattta gaaactataa acatgtcgcc gctatctggt ataaccctga
    65521 tgcacttttc ttcgcaggaa gttggtattt tgcgatacag gattgatctt tagaataacc
    65581 ccatttcaca ttaaactcgc cttccgcact caagccagaa acataagcaa taccatcttc
    65641 acctacaatg ccggccatat tttcagtgtc tttgctataa ctaatcacag cgccgaaggg
    65701 gatgggttta ccctctttct gttttaaggt tactaatcct ttatccccgc tcttcactgc
    65761 caatgtcgca ggcacaacgg ctccagctgt cggtacaacc aattgactag tttgtggtaa
    65821 gtcgtaattc ttacctatcg ttttttcatc cagaataaca tcgtctcttc ggtatggctg
    65881 cactggcagt attgcatacc cacgaaaatc agtactcagt cccggccagc cattaacgtt
    65941 aacccccgtt gctccgggtg cctctataag ggctaatgtc ccatccacat tttgaccaaa
    66001 tgtaagtcca tcccttgtaa taagtatccc accgcttaaa tttgcaccta gctgtttcat
    66061 gttttcgctg gagctgtaat ggctactaaa ctgaccatat gatccatttt tgccaacttg
    66121 cacaatgttc atcgtgctct tcggttgtga ctgatgagcg ttagttatat ttgtcgctac
    66181 atcccaccat acaccaccac tattctgacc tgataatgaa acctgatagt tattaccctg
    66241 ctctcccgaa cgactcatct gtgaagacgc ataaatttga ttttcgtcac ccagcgataa
    66301 tggcactgaa atacccacgc ttaatttccg atctactttc catttatgcg atgtcagcaa
    66361 gtcgtttttt gcccagttaa tggagaatgt tatgcctctc cataccgtac tataactcag
    66421 gctggtagac cgcgtgtttc ctgtgccccg ggaagaatta tacctcgtgg tattgaagtt
    66481 caaattcccg atattaccaa ggggctggga taaggatatc gtagagccat cgtgactatt
    66541 cttatctctt tttctgttcc gttcatcctg tgcacttact gcttcagtca gtgtggcata
    66601 acctgatgca taacggttcg tggttataga
//
LOCUS       DAJTRD010000010        65688 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTRD010000010 DAJTRD010000000
VERSION     DAJTRD010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 65688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65688
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.010"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="OO315_000716"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="OO315_000716"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5889742.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO315_000717"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO315_000717"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5889743.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO315_000718"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO315_000718"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5889744.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO315_000719"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO315_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5889745.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO315_000720"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO315_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5889746.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO315_000721"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO315_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5889747.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="OO315_000722"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="OO315_000722"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5889748.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="OO315_000723"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="OO315_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5889749.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="OO315_000724"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="OO315_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5889750.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTNKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="OO315_000725"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="OO315_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5889751.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="OO315_000726"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="OO315_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5889752.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="OO315_000727"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="OO315_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5889753.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO315_000728"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO315_000728"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5889754.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="OO315_000729"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="OO315_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5889755.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /locus_tag="OO315_000730"
     CDS             19304..19369
                     /locus_tag="OO315_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889756.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="OO315_000731"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="OO315_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5889757.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="OO315_000732"
     CDS             complement(19753..20376)
                     /locus_tag="OO315_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5889758.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO315_000733"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO315_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5889759.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO315_000734"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO315_000734"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5889760.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="OO315_000735"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="OO315_000735"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5889761.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="OO315_000736"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="OO315_000736"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5889762.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /gene="cdaR"
                     /locus_tag="OO315_000737"
                     /pseudo
     CDS             25215..26371
                     /gene="cdaR"
                     /locus_tag="OO315_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26460..26846)
                     /locus_tag="OO315_000738"
     CDS             complement(26460..26846)
                     /locus_tag="OO315_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5889763.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO315_000739"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO315_000739"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5889764.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO315_000740"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO315_000740"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5889765.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO315_000741"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO315_000741"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5889766.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="OO315_000742"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="OO315_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5889767.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="OO315_000743"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="OO315_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5889768.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="OO315_000744"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="OO315_000744"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5889769.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="OO315_000745"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="OO315_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5889770.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="OO315_000746"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="OO315_000746"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5889771.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="OO315_000747"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="OO315_000747"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5889772.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="OO315_000748"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="OO315_000748"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5889773.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="OO315_000749"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="OO315_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5889774.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="OO315_000750"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="OO315_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5889775.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="OO315_000751"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="OO315_000751"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5889776.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="OO315_000752"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="OO315_000752"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5889777.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="OO315_000753"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="OO315_000753"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5889778.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="OO315_000754"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="OO315_000754"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5889779.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="OO315_000755"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="OO315_000755"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5889780.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="OO315_000756"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="OO315_000756"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5889781.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="OO315_000757"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="OO315_000757"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5889782.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="OO315_000758"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="OO315_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5889783.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="OO315_000759"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="OO315_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5889784.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="OO315_000760"
     CDS             53914..54303
                     /locus_tag="OO315_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5889785.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="OO315_000761"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="OO315_000761"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5889786.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO315_000762"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO315_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5889787.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="OO315_000763"
     CDS             complement(55962..56162)
                     /locus_tag="OO315_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5889788.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="OO315_000764"
     CDS             56328..56873
                     /locus_tag="OO315_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5889789.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="OO315_000765"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="OO315_000765"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5889790.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="OO315_000766"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="OO315_000766"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5889791.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="OO315_000767"
     CDS             complement(58171..58995)
                     /locus_tag="OO315_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5889792.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO315_000768"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO315_000768"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5889793.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO315_000769"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO315_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5889794.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO315_000770"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO315_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5889795.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO315_000771"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO315_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5889796.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO315_000772"
     CDS             complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO315_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5889797.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO315_000773"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO315_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5889798.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="OO315_000774"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="OO315_000774"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5889799.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT        15533 a        16277 c        18097 g        15781 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac caataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcctgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggggcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgctaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag gcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaacaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcggcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttgac agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc aactgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt tattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctgtgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgatggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacacctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaatatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttaagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctaccg
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagctat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcaaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcaag tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa ataggaaagt gtaatatacg
    65581 ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa tttatcagac
    65641 aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacga
//
LOCUS       DAJTRD010000011        64193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTRD010000011 DAJTRD010000000
VERSION     DAJTRD010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.011"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            131..574
                     /locus_tag="OO315_000775"
     CDS             131..574
                     /locus_tag="OO315_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5889800.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            810..1628
                     /gene="ybhA"
                     /locus_tag="OO315_000776"
     CDS             810..1628
                     /gene="ybhA"
                     /locus_tag="OO315_000776"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5889801.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1629..2687)
                     /gene="modC"
                     /locus_tag="OO315_000777"
     CDS             complement(1629..2687)
                     /gene="modC"
                     /locus_tag="OO315_000777"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5889802.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2690..3379)
                     /gene="modB"
                     /locus_tag="OO315_000778"
     CDS             complement(2690..3379)
                     /gene="modB"
                     /locus_tag="OO315_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5889803.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3379..4152)
                     /gene="modA"
                     /locus_tag="OO315_000779"
     CDS             complement(3379..4152)
                     /gene="modA"
                     /locus_tag="OO315_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5889804.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="OO315_000780"
     CDS             complement(4319..4468)
                     /gene="acrZ"
                     /locus_tag="OO315_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5889805.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4597..5385
                     /gene="modE"
                     /locus_tag="OO315_000781"
     CDS             4597..5385
                     /gene="modE"
                     /locus_tag="OO315_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5889806.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5453..6925
                     /gene="modF"
                     /locus_tag="OO315_000782"
     CDS             5453..6925
                     /gene="modF"
                     /locus_tag="OO315_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5889807.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7186..8202
                     /gene="galE"
                     /locus_tag="OO315_000783"
     CDS             7186..8202
                     /gene="galE"
                     /locus_tag="OO315_000783"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5889808.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8212..9258
                     /gene="galT"
                     /locus_tag="OO315_000784"
     CDS             8212..9258
                     /gene="galT"
                     /locus_tag="OO315_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5889809.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9262..10410
                     /gene="galK"
                     /locus_tag="OO315_000785"
     CDS             9262..10410
                     /gene="galK"
                     /locus_tag="OO315_000785"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5889810.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10404..11444
                     /gene="galM"
                     /locus_tag="OO315_000786"
     CDS             10404..11444
                     /gene="galM"
                     /locus_tag="OO315_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5889811.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11647..12399
                     /gene="gpmA"
                     /locus_tag="OO315_000787"
     CDS             11647..12399
                     /gene="gpmA"
                     /locus_tag="OO315_000787"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5889812.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="OO315_000788"
     CDS             complement(12566..13618)
                     /gene="aroG"
                     /locus_tag="OO315_000788"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5889813.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLSAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13934..14314
                     /locus_tag="OO315_000789"
     CDS             13934..14314
                     /locus_tag="OO315_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5889814.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14428..15369
                     /gene="zitB"
                     /locus_tag="OO315_000790"
     CDS             14428..15369
                     /gene="zitB"
                     /locus_tag="OO315_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5889815.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="OO315_000791"
     CDS             complement(15366..16085)
                     /gene="pnuC"
                     /locus_tag="OO315_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5889816.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="OO315_000792"
     CDS             complement(16123..17166)
                     /gene="nadA"
                     /locus_tag="OO315_000792"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5889817.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDAFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17415..17490)
                     /locus_tag="OO315_000793"
     tRNA            complement(17415..17490)
                     /locus_tag="OO315_000793"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Lys,seq:ttt)
     gene            complement(17494..17569)
                     /locus_tag="OO315_000794"
     tRNA            complement(17494..17569)
                     /locus_tag="OO315_000794"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17534..17536),aa:Val,seq:tac)
     gene            complement(17621..17696)
                     /locus_tag="OO315_000795"
     tRNA            complement(17621..17696)
                     /locus_tag="OO315_000795"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17661..17663),aa:Lys,seq:ttt)
     gene            complement(17699..17774)
                     /locus_tag="OO315_000796"
     tRNA            complement(17699..17774)
                     /locus_tag="OO315_000796"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17739..17741),aa:Val,seq:tac)
     gene            complement(17910..17985)
                     /locus_tag="OO315_000797"
     tRNA            complement(17910..17985)
                     /locus_tag="OO315_000797"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17950..17952),aa:Lys,seq:ttt)
     gene            complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="OO315_000798"
     CDS             complement(18150..18941)
                     /gene="cpoB"
                     /locus_tag="OO315_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5889818.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18951..19472)
                     /gene="pal"
                     /locus_tag="OO315_000799"
     CDS             complement(18951..19472)
                     /gene="pal"
                     /locus_tag="OO315_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5889819.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="OO315_000800"
     CDS             complement(19507..20799)
                     /gene="tolB"
                     /locus_tag="OO315_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5889820.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20932..22173)
                     /gene="tolA"
                     /locus_tag="OO315_000801"
     CDS             complement(20932..22173)
                     /gene="tolA"
                     /locus_tag="OO315_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5889821.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22238..22666)
                     /gene="tolR"
                     /locus_tag="OO315_000802"
     CDS             complement(22238..22666)
                     /gene="tolR"
                     /locus_tag="OO315_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5889822.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22670..23362)
                     /gene="tolQ"
                     /locus_tag="OO315_000803"
     CDS             complement(22670..23362)
                     /gene="tolQ"
                     /locus_tag="OO315_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5889823.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23359..23763)
                     /gene="ybgC"
                     /locus_tag="OO315_000804"
     CDS             complement(23359..23763)
                     /gene="ybgC"
                     /locus_tag="OO315_000804"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5889824.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23913..24206)
                     /gene="ybgE"
                     /locus_tag="OO315_000805"
     CDS             complement(23913..24206)
                     /gene="ybgE"
                     /locus_tag="OO315_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5889825.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24206..24319)
                     /gene="cydX"
                     /locus_tag="OO315_000806"
     CDS             complement(24206..24319)
                     /gene="cydX"
                     /locus_tag="OO315_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5889826.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24334..25473)
                     /gene="cydB"
                     /locus_tag="OO315_000807"
     CDS             complement(24334..25473)
                     /gene="cydB"
                     /locus_tag="OO315_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5889827.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25489..27057)
                     /gene="cydA"
                     /locus_tag="OO315_000808"
     CDS             complement(25489..27057)
                     /gene="cydA"
                     /locus_tag="OO315_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5889828.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27906..30539)
                     /gene="mngB"
                     /locus_tag="OO315_000809"
     CDS             complement(27906..30539)
                     /gene="mngB"
                     /locus_tag="OO315_000809"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5889829.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30557..32473)
                     /gene="mngA"
                     /locus_tag="OO315_000810"
     CDS             complement(30557..32473)
                     /gene="mngA"
                     /locus_tag="OO315_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5889830.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32643..33365
                     /gene="mngR"
                     /locus_tag="OO315_000811"
     CDS             32643..33365
                     /gene="mngR"
                     /locus_tag="OO315_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5889831.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33468..34337)
                     /gene="sucD"
                     /locus_tag="OO315_000812"
     CDS             complement(33468..34337)
                     /gene="sucD"
                     /locus_tag="OO315_000812"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5889832.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34337..35503)
                     /gene="sucC"
                     /locus_tag="OO315_000813"
     CDS             complement(34337..35503)
                     /gene="sucC"
                     /locus_tag="OO315_000813"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5889833.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35597..36814)
                     /gene="odhB"
                     /locus_tag="OO315_000814"
     CDS             complement(35597..36814)
                     /gene="odhB"
                     /locus_tag="OO315_000814"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5889834.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36829..39630)
                     /gene="sucA"
                     /locus_tag="OO315_000815"
     CDS             complement(36829..39630)
                     /gene="sucA"
                     /locus_tag="OO315_000815"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5889835.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39931..40647)
                     /gene="sdhB"
                     /locus_tag="OO315_000816"
     CDS             complement(39931..40647)
                     /gene="sdhB"
                     /locus_tag="OO315_000816"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5889836.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40663..42429)
                     /gene="sdhA"
                     /locus_tag="OO315_000817"
     CDS             complement(40663..42429)
                     /gene="sdhA"
                     /locus_tag="OO315_000817"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5889837.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42429..42776)
                     /gene="sdhD"
                     /locus_tag="OO315_000818"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42429..42776)
                     /gene="sdhD"
                     /locus_tag="OO315_000818"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5889838.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42770..43174)
                     /gene="sdhC"
                     /locus_tag="OO315_000819"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42770..43174)
                     /gene="sdhC"
                     /locus_tag="OO315_000819"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5889839.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43555..43662
                     /locus_tag="OO315_000820"
     CDS             43555..43662
                     /locus_tag="OO315_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889840.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43868..45151
                     /gene="gltA"
                     /locus_tag="OO315_000821"
     CDS             43868..45151
                     /gene="gltA"
                     /locus_tag="OO315_000821"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5889841.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45542..46108
                     /locus_tag="OO315_000822"
     CDS             45542..46108
                     /locus_tag="OO315_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5889842.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46159..48618
                     /locus_tag="OO315_000823"
     CDS             46159..48618
                     /locus_tag="OO315_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5889843.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48633..49364
                     /locus_tag="OO315_000824"
     CDS             48633..49364
                     /locus_tag="OO315_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5889844.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49361..50437
                     /locus_tag="OO315_000825"
                     /pseudo
     CDS             49361..50437
                     /locus_tag="OO315_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50589..51635
                     /locus_tag="OO315_000826"
     CDS             50589..51635
                     /locus_tag="OO315_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5889845.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51632..52423)
                     /gene="nei"
                     /locus_tag="OO315_000827"
     CDS             complement(51632..52423)
                     /gene="nei"
                     /locus_tag="OO315_000827"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5889846.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52459..53193)
                     /gene="pxpA"
                     /locus_tag="OO315_000828"
     CDS             complement(52459..53193)
                     /gene="pxpA"
                     /locus_tag="OO315_000828"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5889847.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53183..54115)
                     /gene="pxpC"
                     /locus_tag="OO315_000829"
                     /pseudo
     CDS             complement(53183..54115)
                     /gene="pxpC"
                     /locus_tag="OO315_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54109..54765)
                     /gene="pxpB"
                     /locus_tag="OO315_000830"
     CDS             complement(54109..54765)
                     /gene="pxpB"
                     /locus_tag="OO315_000830"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5889848.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54788..55531)
                     /gene="ybgI"
                     /locus_tag="OO315_000831"
     CDS             complement(54788..55531)
                     /gene="ybgI"
                     /locus_tag="OO315_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5889849.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55802..57283
                     /gene="dtpD"
                     /locus_tag="OO315_000832"
     CDS             55802..57283
                     /gene="dtpD"
                     /locus_tag="OO315_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5889850.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57433..58851)
                     /gene="phrB"
                     /locus_tag="OO315_000833"
     CDS             complement(57433..58851)
                     /gene="phrB"
                     /locus_tag="OO315_000833"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5889851.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58848..59357)
                     /locus_tag="OO315_000834"
     CDS             complement(58848..59357)
                     /locus_tag="OO315_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5889852.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59486..59773
                     /locus_tag="OO315_000835"
     CDS             59486..59773
                     /locus_tag="OO315_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5889853.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59828..60208)
                     /locus_tag="OO315_000836"
     CDS             complement(59828..60208)
                     /locus_tag="OO315_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889854.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60211..63597)
                     /locus_tag="OO315_000837"
     CDS             complement(60211..63597)
                     /locus_tag="OO315_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5889855.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(63616..63819)
                     /locus_tag="OO315_000838"
                     /pseudo
     CDS             complement(63616..63819)
                     /locus_tag="OO315_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15694 a        17278 c        15620 g        15601 t
ORIGIN      
        1 tgccgaagaa gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg
       61 actgttaatt gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt
      121 cggcctttgc atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat
      181 gagatgggag gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa
      241 aattcgcatt tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact
      301 ggataacttg aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag
      361 gacaattgca gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga
      421 cccaccgcca tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg
      481 cagtgatttc actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg
      541 tgacggacga gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcgaaatg
      601 ccacttgtaa ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg
      661 ctcctttgca tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg
      721 ctgaattaaa acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga
      781 aaattcttaa ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag
      841 acggcacctt attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc
      901 gcgctcgcga agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc
      961 atccttttta tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt
     1021 tgtatgatta tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc
     1081 tgcaactcat tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg
     1141 caatggtcta tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc
     1201 tgccgccgga acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac
     1261 aagttaacgc cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt
     1321 ttggtaagca tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg
     1381 ttgatattgc acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc
     1441 aaggttggtc gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc
     1501 tggaagccgc tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg
     1561 ccaacattgt gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc
     1621 tgatttaatc aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt
     1681 ttgatcgcca gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg
     1741 acttccagct ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc
     1801 aatacgttac gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc
     1861 tgaatgcgga tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc
     1921 tgatcgccca gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact
     1981 ttcagaatgc tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac
     2041 acttcctcca gcgcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct
     2101 gccagatgga ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc
     2161 cgtgtcagcc gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc
     2221 agcggttcat ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg
     2281 cgctgttttt cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct
     2341 aaaagcgcca ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga
     2401 ttgccacgca ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga
     2461 cgcttttccg gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg
     2521 acaatccgcc ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt
     2581 ccggcaccgg agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg
     2641 gtcaggcaat ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg
     2701 cccgttcacg gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg
     2761 caatagaaat aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg
     2821 tatacatggc agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg
     2881 ttgcaccaaa ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg
     2941 gtaaggtcag cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc
     3001 ttgcggcctg ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca
     3061 tcagcggaaa cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg
     3121 taataccaaa ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta
     3181 ataataagta accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga
     3241 gcagagcttt gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg
     3301 gcaggctaaa cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt
     3361 ctggatcggt cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc
     3421 ctgcggtccc ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac
     3481 aacagcaacc ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt
     3541 tacccctttg ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg
     3601 ttcgaccagc gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt
     3661 atcccatgcg cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc
     3721 cggatcgcca accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc
     3781 gatggtgaaa tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag
     3841 cagtgtctga cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt
     3901 ctgatcggca gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt
     3961 agatgacgaa gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc
     4021 aatgtcctgc attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc
     4081 tgccagtgca ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca
     4141 tttacgagcc attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa
     4201 ctcggaattt tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg
     4261 caggaaaagc aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc
     4321 aatgattttg tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac
     4381 caagaccgta aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga
     4441 ataccagact ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc
     4501 taaccgctaa gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt
     4561 tttccgtcac aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc
     4621 cttaagctcc aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt
     4681 gcgctttccg gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc
     4741 tgggatgcca ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca
     4801 ggcggtaaag gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc
     4861 tatgacttac tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc
     4921 ctgccgctga acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt
     4981 aaccagtggt tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat
     5041 gtcttactgg ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca
     5101 cgtctggggc tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc
     5161 attactcagg acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt
     5221 catattgagc gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca
     5281 ctgtgcgcca cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg
     5341 gcctacttta atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt
     5401 gttatgaaac acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc
     5461 gttgcaaatt ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca
     5521 gctaacgtta aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc
     5581 ggcgctggcc cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca
     5641 gttttcccac atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg
     5701 gcaacggaat aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc
     5761 tgagattatt caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt
     5821 cggtattacc gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa
     5881 aaccctgctg tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt
     5941 cgatggcctg gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca
     6001 gtccggtatt actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca
     6061 gtttgctggc gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct
     6121 ccagcaagca ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc
     6181 ggagccggat gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct
     6241 gaacaatggc gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt
     6301 gaatccaggc gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt
     6361 aagcctgatt actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg
     6421 acgtcgcggc agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag
     6481 tagtttgcat ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta
     6541 ttttgattcg attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca
     6601 gtggctggat attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc
     6661 ctggggacag cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct
     6721 tattctcgat gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt
     6781 tgttgatgtg ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga
     6841 agatgcgcct gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg
     6901 ctatgtgctg acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag
     6961 cgcactctcc ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata
     7021 aaaagcgcaa tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg
     7081 gctaaattct tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt
     7141 gttatgctat ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt
     7201 accggtggta gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat
     7261 gatgtcatca ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag
     7321 cgtttaggcg gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg
     7381 accgagatcc tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc
     7441 gtcgccgaat cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg
     7501 cgcctgatta gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc
     7561 accgtttatg gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg
     7621 caaagccctt atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa
     7681 gcccagccgg actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg
     7741 tcgggcgata tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc
     7801 caagttgctg taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa
     7861 gatggtactg gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg
     7921 gcgatggaaa aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta
     7981 ggcagcagcg tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac
     8041 tatcattttg caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa
     8101 gccgaccgtg aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc
     8161 tggcactggc agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa
     8221 tttaatcccg ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg
     8281 gtttcaccgc accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag
     8341 gtgttacctg cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc
     8401 gataaaaacc ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg
     8461 tctgacacgc cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc
     8521 ggtaccagcc gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc
     8581 gttgcggcat tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa
     8641 acgtacccgt gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg
     8701 catccgcacg gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac
     8761 cgcctgcaaa aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag
     8821 cgcgagctgg cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg
     8881 ccttactggg ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg
     8941 atcaccgatt tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc
     9001 agtcgttatg acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca
     9061 ccatttaatg gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg
     9121 ctgcgctccg ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag
     9181 cgagacctga ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt
     9241 cgcgaatccg gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac
     9301 gcatttggct accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt
     9361 gaacacaccg actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg
     9421 atcagctgtg caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat
     9481 cagctcgacg agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg
     9541 aactacgttc gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg
     9601 gacatggtga tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg
     9661 gaagtcgcgg tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa
     9721 atcgcgctta acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg
     9781 gatcagctaa tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca
     9841 ctggggacta aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac
     9901 ttcaaacgta ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt
     9961 gcgcgtttct tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt
    10021 gcacatgagc tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc
    10081 cgcaccgttg aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg
    10141 atggccgagt ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac
    10201 actctggtag aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc
    10261 ggcggatttg gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag
    10321 caagctgtcg ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt
    10381 aaaccatcac aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc
    10441 agccgtaccg actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact
    10501 ggggtgcgac tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc
    10561 tgctcggctg tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta
    10621 ttggtcgtta tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga
    10681 cgctttcgcc aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac
    10741 gtcgctggca gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg
    10801 atggcgatca gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg
    10861 ataaccgtat ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga
    10921 ctaatcacgt ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc
    10981 agattctggc ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga
    11041 aatctgtcgc cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc
    11101 ttgccgacga cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca
    11161 aaggcgatgg caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga
    11221 aggtctacac caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac
    11281 cgtcgcgggg aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc
    11341 tgccggacag cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg
    11401 aagagtattc cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca
    11461 tgagggcttt atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca
    11521 atattcacaa caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc
    11581 tgttgcttcg tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt
    11641 aagcatatgg ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa
    11701 gaaaaccgtt tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca
    11761 aaagcagcag gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct
    11821 gtgctgaaac gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg
    11881 ctgcccgttg agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg
    11941 aacaaagcgg aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt
    12001 tttgcagtga ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg
    12061 cgttacgcga aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt
    12121 gaccgcgtga tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc
    12181 gtaatcatcg ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg
    12241 agcgaagaag agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc
    12301 gacgagaatt tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg
    12361 aaagcagcag ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg
    12421 agaaagccga ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat
    12481 tgcctgatgc gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc
    12541 tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt
    12601 gacgtaacag agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt
    12661 aggccagcgg ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca
    12721 tcacgccaat aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat
    12781 ccatctgctt tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag
    12841 ctgacagacc tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt
    12901 taggctcttt accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa
    12961 tcgccgaatg cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa
    13021 tggcatcgat agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag
    13081 aaagaccaga cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc
    13141 cccagctcat caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg
    13201 ctggcagacc gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt
    13261 tgatctggaa gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg
    13321 tacgcggctt ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac
    13381 gcagcgccag caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg
    13441 ggccaatcac caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc
    13501 gggcatgggc aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga
    13561 caggaggaag taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt
    13621 ctgttccagt gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg
    13681 tcagaatgtg taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa
    13741 aacgccagat tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg
    13801 tttgttaatt atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca
    13861 atgccttata ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata
    13921 acaggagcat cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag
    13981 ccttgccagc ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc
    14041 aaacgccgca gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa
    14101 taacgtcgac aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg
    14161 ttcttcaatg aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa
    14221 agatggtcga tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga
    14281 tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag
    14341 agcggagtct ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa
    14401 gtgagaataa ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac
    14461 ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt
    14521 atgctggtag aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg
    14581 ggtcatatgt tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca
    14641 cgtcgtcctc ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg
    14701 tttgtgaatg ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa
    14761 cgcttccgca cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg
    14821 ttgctggcaa atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc
    14881 aacgtacgag cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt
    14941 attgccgcgt tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata
    15001 ctggtgtcgc ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa
    15061 ttacttgaag gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg
    15121 gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg
    15181 gtgatgacgt tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag
    15241 atccaacact acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat
    15301 caaccttgtc ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat
    15361 caccattaat gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg
    15421 ctgccgttga gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc
    15481 tgaagtgcaa aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc
    15541 acatacttac gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc
    15601 cagaacggga aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc
    15661 atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc
    15721 agaccaatcg aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag
    15781 cgaattttca actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa
    15841 atattcgcgg caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag
    15901 aaaataatgc caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc
    15961 agactggcaa ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag
    16021 agatcataac cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa
    16081 tccatctttc ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc
    16141 cgcaaaatcc agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac
    16201 atgaacctcg tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt
    16261 catggccatc cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg
    16321 tgcttccagt aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc
    16381 ggttgccaca ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt
    16441 ggaaccgacc gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat
    16501 ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa atgcatcatg
    16561 cacaatgcag gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg
    16621 ccccagatgt ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat
    16681 aagttcgacg gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga
    16741 agtgttggcg tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc
    16801 ttcaacaggg cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt
    16861 tttttccgga ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa
    16921 agtagaagcg ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc
    16981 accggtttct tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac
    17041 cgcattacgt tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat
    17101 gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac
    17161 gctcatctta ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc
    17221 gctgttatcg gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag
    17281 tcactcgaat tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga
    17341 tgtcatcagg tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg
    17401 gcgctttttt gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt
    17461 caactgctct accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc
    17521 cgaccccctc cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct
    17581 ggatactaca aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc
    17641 gaacctgcga ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg
    17701 gtgggtgatg acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc
    17761 tgagctaatc acccgacact acgctggata ctacaactaa ctatgttagt gggggtcgtg
    17821 caggatgact cgacttcgtc tcgcccttcg gcgccgttgc tgatgcaacg ttatccttca
    17881 cgttttacta ccgcttccac cttagcgatt ggtgggtcgt gcaggattcg aacctgcgac
    17941 caattgatta aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt
    18001 tcggtttgtt tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg
    18061 caacaaaaat ttcgatgcag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa
    18121 taatgcgact tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt
    18181 ttagcgccat cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt
    18241 gcggtgtcac ctttgtcctg catgatgacg ccgactttaa acatcgcatc agcagccttt
    18301 ggtgacttcg gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta
    18361 cccttgttgt agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca
    18421 gggtaatttt tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc
    18481 tgcaccagcg caatagctgc attgtaatcc gtgtttgcat cgccgctttt caccggcgcg
    18541 ccagcattcg cagtaccagc atcagctgtc ggcgtcgttg atgccgtcgc accgctttga
    18601 tcgctgctgg ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg
    18661 atctgctttt gccgctccac gacctgattc agttgatact gattttcctg aatctgacca
    18721 cgcagggaat caatatcgga ttgattatca gaaagttgtt gctggagttg ggttaaaagc
    18781 tggctgtgag cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag
    18841 ctgacactac tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt
    18901 aacgacagac tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc
    18961 agtaccgcac gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt
    19021 tctttaccgt aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac
    19081 atcttaacgg cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt
    19141 tcgtccgcgt gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca
    19201 tgtgcatcca gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa
    19261 acgatgttgt tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg
    19321 ttgccgttgc cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg
    19381 tcattgctgg cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc
    19441 atcagccctt tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa
    19501 ttattatcac agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag
    19561 acgcgctttg aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga
    19621 agagctgtag attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt
    19681 ggacgacaga acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc
    19741 accattggag ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt
    19801 ctgcgaacct tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac
    19861 ctgcggacga ccggcctggt cagaagtaaa tgccaggttc tggctatccg ggaaccaggt
    19921 cggttcggta ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc
    19981 cattacgtac aggttcagac taccggtttt cgacaaggcg aatgccagtt tgctgccgtc
    20041 tggcgagaat gcaggtgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc
    20101 atttgccagc gtctgaataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag
    20161 tttagaaccg tctggtgacc acgccggaga catcagcggc tgcggtgaac ggtgaacgac
    20221 aaactggttg taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt
    20281 ggtctgaaca acgtaggcaa tacgggtacg gaacgcacct ttaatgccgg tcagtttttc
    20341 aaacacttca tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta
    20401 cgagttctga gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac
    20461 attgtaagaa ccatccggat tcggagtgac ttgaccgaca actacagcgt caatgcccag
    20521 tgcggaccat gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc
    20581 gcgatctaac ggattaaatt taccgctgtt acgcaagtca gcagcaacga tgccgccaat
    20641 atcttcaggt gccgcaccag gccccgccca ctggaaagga acaacaccaa taggacgacc
    20701 ggaatctaca ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag
    20761 tatgagaaaa ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc
    20821 gatggcccac gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac
    20881 cccgtggcaa ccggtgcctg atgttgaccg tccgaacagt caacatcgcg attacggttt
    20941 gaagtccaat ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat
    21001 cttcgcaagt ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg
    21061 tttgatatcc agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc
    21121 tgcataggac gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata
    21181 gttattgata tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga
    21241 agcattgttc cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc
    21301 accgaaaata tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc
    21361 ttttgccgct gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc
    21421 tgctgcctta tcagcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc
    21481 tgccgctttc ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt
    21541 ggctttttca gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc
    21601 cgcttccgct ttcttcttca gtgccgcagc ggctacctcg gcttttttct gcgcttcggc
    21661 tgcggctttg gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc
    21721 tgcttcttcc gcagctttag catctgccgc cgctttcgct gccgccactt cagcttgctt
    21781 ctgctttaac tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc
    21841 taaccgctct ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag
    21901 ttcttcagca gcctgctgtt ccttcatctt gcgctgttca tcagaacgct tcgcgcttga
    21961 ttcctggctt tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc
    22021 gtcgatggac gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact
    22081 ccagatcagc gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt
    22141 gagcttgtcg ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg
    22201 ttacccgctc tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac
    22261 caaccgattt cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag
    22321 gcacatcttt tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg
    22381 acacttccgc caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca
    22441 cggtgtactg accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca
    22501 ccgcctgtga ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg
    22561 ctgtcgccat aaagatcagc aacagcacca gcagtacgtc cagcaacgga acaatgttga
    22621 tttcggactt gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt
    22681 tgctctcgct aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt
    22741 tgtcgtaatt cagttccagt ttgtttacgc gctggttgag acggttataa gccattaccg
    22801 ccggaattgc ggcgaacaga ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg
    22861 caaccatttg cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc
    22921 cccagaccgt accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg
    22981 gaatgtgcgt ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg
    23041 ccccttccac tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt
    23101 tgaacccgct gtaaaagatt tgttccgaac cagtcagatt atcccgtttc ccctgactct
    23161 cttgatagag gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt
    23221 cacgcgccgc tgcgttaaga atacgggttc gctggataat aatggcccag gatgcgattg
    23281 aaaaaccaat caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat
    23341 caaggatatt catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg
    23401 aggcttcatt ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt
    23461 attctcggcg ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat
    23521 ttcagtctgt atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat
    23581 tttacgtacc acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg
    23641 acgcagcatc tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac
    23701 accaccggca tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt
    23761 cactttacat cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg
    23821 cgcgattgag gtttgggaat gagggcaagt taagggcgcg taaaaattta tgggcctaga
    23881 gcggcccata attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca
    23941 atgagaacaa tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga
    24001 cgaaagccca cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc
    24061 cagatttcca gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct
    24121 gctaacagaa gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca
    24181 tacaaggtcg cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga
    24241 cgtgttcaag cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa
    24301 tccatgcgaa ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc
    24361 aatatcttct ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag
    24421 aatgatcggt accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga
    24481 agttgcatcc cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg
    24541 gaacattgcg ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa
    24601 cgcccacgcg gctttatcca tacgtgcagt caggatggtc agcagcagca gaaccacacc
    24661 cagtgccgga atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc
    24721 ttcacgaacc acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt
    24781 cacgacataa ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag
    24841 tgtcaccagc gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt
    24901 acgcatttgc agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc
    24961 cagcaggccg aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata
    25021 ttcatcaacg ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag
    25081 cggcggaacg aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc
    25141 ttcaatcttg gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag
    25201 gatcatcgcc acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa
    25261 gagtgcgccg cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga
    25321 gttaatcata attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac
    25381 ccccatgtcg aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca
    25441 ccagataaaa cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg
    25501 ctgagtagtc gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc
    25561 gaggcgtgca aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc
    25621 gcaaatcagc accattgaga agatgagatc gcctgcggtc agtgacgaat tcgccacagc
    25681 tgtcggcagc acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc
    25741 cgcttctaca gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt
    25801 tttctcgcca atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagcag
    25861 caggaagcca cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat
    25921 ggagtctttg gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata
    25981 gcgtttcagc agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac
    26041 cgtctggtcg gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt
    26101 acgaatgcgt tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac
    26161 ggaacgcgtt gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc
    26221 ctcttcctga tcaggaatgc cgaacaaagt aaaggcagca ggcgcaggtt gcgtttccca
    26281 ctcggcttca atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc
    26341 atcacccaga acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa
    26401 ggagcgttta gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag
    26461 gatgaacatc gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc
    26521 aaccgggtta agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt
    26581 gaaatcggac gcgattgggt tttgcatcca gccgttcgca accagaatcc acagtgcgga
    26641 caggtttgaa ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg
    26701 atcccaaccg aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc
    26761 ttcgattgcc agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt
    26821 agtcccgaac tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc
    26881 aaacaacttg ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta
    26941 gaccgtttcc ataatggcca ggaggaacgc cataccgagc gtcagtggca caaaaaggaa
    27001 gtggtacatc gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat
    27061 gactccttgc tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc
    27121 atgccccaat agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac
    27181 ggcgtaaaga aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt
    27241 acgccgcaaa atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg
    27301 gtgatcaatt tatagctaaa ttgccgcctt tcagccaatt tgatcgagaa caatttatct
    27361 ctttttgatg cccatttcca agacttatac attgataaat atcaatttct acaaattatg
    27421 ttaatcatgt ttcaacaagg taactttagt gatatttatg tttaaaatgt gtgaattata
    27481 agactgaaat tagtttcggt tggcaattaa agataaaaag agacgaagca aaaacagcac
    27541 ggttgacctt aattcttttt ttggtcactg gctaatacga cgcagagttt caggattatt
    27601 aataaaggca taaatctccc ccccgccgat attaacattt aattaactta taattatatt
    27661 ataattaact cacccgtggt tattattttg ttaaaaataa agccacatat acgtagcttg
    27721 agagtgaaga taaacacaaa attacctgat gcgctacgct tgccagtgtc tacatcatct
    27781 ctgcaatata ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg
    27841 gcatgaacaa agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt
    27901 gctattcagg caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat
    27961 agatttgaac cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa
    28021 gaaatcacct cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta
    28081 aacagccgta aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggcat
    28141 cccactgggg gcattttcaa caaactataa ctttccggca cgttgaatcc ggctttgttg
    28201 agcttcatcg catcccatgg gattttgttg taacactgta ctggagtcag ccatgctcgc
    28261 gcctgctgag ctacaccagc ggcggttggc gtaccggtat aactcaataa acttaggcga
    28321 caagaaagca gaccacgtag ttgtgagtcc gggactggca ttttaattcc cgaaggccgc
    28381 ctaggcctta aaagcagatc ttctttcccc agtaagccca cgccgcgcag caacgtaatg
    28441 gcaaaggttt tcttctcttc accgatgact tcaaattcac gtagcccttc gctaaagaca
    28501 gccatgccgt tacgcccttc ctgtaaggct gcatagttga gcatattcca taccggaacc
    28561 ggcgcttctt tccagctttc ttgctgccag ttgttcattg cactgtcgtt cacggggcgc
    28621 gttagcgaac caaactgggt atctgccaga acactatcgg tgttaaaaga tgtagggatc
    28681 aggacgcgaa ggcgatgatc gtcagcctgg ttatcaaggt tgatatccac atcaatgcgc
    28741 ctgttgttat gactaagagt gacaaccaac tcgacatcta ctctgccagt ggattgtctt
    28801 gcgctgcgtt gttgcagatt acgcggcacc gccatttcat agcggataac agccctgctc
    28861 tgccaggcct catgaataat ctcgcattgc ggtttcgcat tcgccgaagt aattacccac
    28921 tcttcttttg cgggtgaata gtcatattca tcaccatcat cagagctttc ttcaatttgc
    28981 aacacccggt catagcgcac accgctatct ttatctacca gttgtagcgt tccatcctca
    29041 ttgagcgcaa tttgccagaa agcattttcc agtatccctt cagcgtcact ttttgccgca
    29101 atcacgttgc caggctgatt cgcttcgata taaagcgtgc gatagcccat agaagggaca
    29161 atctggttga tctgtatatc aaactccata aagggatcgt aattaccgta atgaactatt
    29221 tgccgatcga ttaggcctgg atcgatctca cgggcatggc gaataaaata cggtacaggc
    29281 tgaccgcgat cgtctcgtag attaaactgg ctggcgcgca gccgcacagt ggtgttgatc
    29341 acttcttccc gcggccaggg catcaggtta aacaggacga gtttgtcggc gtcgctctgc
    29401 ggcatgttgt cggcgatttt acgcatatag aaacgcagca gattatccgc catgtcttca
    29461 gccagttcga agcgggcgac gatttcgcga tgaactttgt cacgacagca gcagccgatg
    29521 ctgtcgtggg catgattttt taagatctct ttccacattt tctccagcaa gccgtggtgg
    29581 tatccaaaac ccaacgtcct ggccagcgtt gccagcggtt ccagcagatt aacaatctta
    29641 ttttcaatac gtgcgtgggc aactttgata tccatgcgcg tggaaccgat ggtgcgatgc
    29701 acgcgcatat atttgccatc aataaattcc cctttcaggg ttgccagatt atctcgctgc
    29761 gcttcgatct tctcaaatac ctcttcaaag cggctcatca caaactttcg ttgaggataa
    29821 atttcgcgca gtttctccat cacttcgaag atattttgct gcaatggcat ctgatcatgc
    29881 ccattcggca gcaaaatctc tttggttaca gacgcttttt ccagcacgtc aaaataactg
    29941 tcgaggcgtt tgcgtaatcc gttttcgtcc gcaggtaagt acttaccgat ggcataaccc
    30001 aacggcatca cctgcgccgt cacttcgcta ccgtcactgc tttgccacaa aaactcggtt
    30061 ttatccgtac cgtggcgctc agaacatcca cgccagaaca tggtgcaggt aatgccaaat
    30121 ccattgtaga tatgcggcag ttgcccggac atgccaaagg aatcgggtaa ataacctatt
    30181 ttcatcggct cgccaaacgc gaggcagtca cgcattccgt acatcagatt acggacgatg
    30241 gattccgcag aaacaatcgt ggtatcggtc tgggtatacc aggggccgat aatcaacttg
    30301 ccggcttcta ccagtttctt caagcggtct ttgttttccg gtttcaccgc gaaataatct
    30361 tcgaggatcg ccgtttgccc gtcgagtacg taatatttgt attcgttgtc ctgttccagt
    30421 cggcacagga tctcttccat attattgacc agcagaatgc gtgactcttc ggtggtgaaa
    30481 taccactccc gatcccagtg catatgcggg gtgatgtgaa cgcgagatac cgctttcatc
    30541 gtcgtttcct ttttgtttat ggcattacgc catcagtcag atagttgcca tgcttaaccg
    30601 cgtgacgccg ccagatcagg agaattgcag tcgagattgc agcccccacc aatgccgcac
    30661 caaaccagcc aattgccgcc ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg
    30721 agaaaatgcc agcaccgggt gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg
    30781 ttaccataga gcccagcaca aacgaaccaa taacccgcag cggatcttca atcgccatcg
    30841 ggatcgcccc ttcggtaata cctgccagcc ctaacagcca ggtggatttc ccggtctcaa
    30901 tttcaaactc tttaaacagg cgcggtgcga gcatcgtgga agcggttacg gtaaatgccg
    30961 aaaccatttt gacggaggcg aaaatggcat acgggccgta aacgccgttc gccattgcgc
    31021 ccaggcagaa tgcataagcg gctttattca ctggccctcc aaggtcaaag gaacacataa
    31081 aaccgagaat ggcacccagc aacagcgcgt tacttcctga cagaccgttc agccaggcgg
    31141 taagcgagtt attgatccag gcgacaggtt cccccaccac aaacagcatc agactgcccg
    31201 ctcccaatgt accgagcacc gggtagagat aaaaagtcag gaagccattg aatttactgc
    31261 taagacgcaa gtgatttttc atccagcgta tcaagtaacc ggctatcaat ccgccaacga
    31321 ccgcgccgag aaacccggag ccgatcatgt tggcggcaag tccagccgca aagcctggcg
    31381 ctaacgccgg tttatctgcc agcgaatagg cggtataggc tgccagcacc ggtaccatca
    31441 aaattccgag cagcccgccg cccagtttgc ggtacatcca cagccaggag ttttcttcat
    31501 taaacagatc ttgtagcccg aagatttgcg acagtaatac cgcgaccgcc agcaccgtcc
    31561 cccccgcgac aatcaacggt acggcaaaag agatcccgct caacagtgcc tgtttcagct
    31621 ccgttttgac gcttttcacc ggttgcgtat cttgctgtaa ggtacgcgtc tcatcgccac
    31681 gctcgagggt aagcgattgt tggatcaacg cttctgcatg gcgaatcggc tcggcaacag
    31741 gcacagaaag cgcaggaatg ccgttaaaac gctcactctc cttgatggcg acttcagccg
    31801 caaaaataca ggcagttgca ctattgagtt gatccgccgt taaacgccct tcaataccgt
    31861 tagcaccttg cttttcaacg tagacattca cgccgagttt gcgtccggct ttttccagat
    31921 actccgcagc catgtaggtg tgagcaatac ccgccgggca ggccgttacg caaacgatag
    31981 ttggcgcgtt ggaaaaagag gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga
    32041 gcaattcatc aggcgtcgtc gccgactgta tacgcgcccg aatctcatca tccgccaggc
    32101 gcgtggtcag cgctgtcagc agttgcatgt gcgtagtacc cgcttcattg aggggaatcg
    32161 ccagcaacac cactaaatca accgcttccg ggccatccac gccttcccac tgaagcggct
    32221 cgctgagtgt ggcgaccgca aacgccgctt cttttaccgc agcagttttg ccatgcggca
    32281 cagccaaccc ttcacttaag gcggtcgggc caaggctttc acggcgatac acttctttca
    32341 gaaattgctc agtactggaa attttcccca gagcagcaag acgttgagtc aacgcgtgaa
    32401 tggcctcttc acggctggta aagcgcgcat tcagacacaa cgcatcgcgg tgggtcagag
    32461 tcgtcaggtt catcatgatc ctcacttgat cgtttttata gtcgcgccag tgtgcccgac
    32521 aaaacaatac cattgcgtaa tcaatttcac ttgattgaaa ttatttgcat tttatttgta
    32581 ttgtttacag gatgagttta tcgggcataa tggctaaatt cgtattaatg agatgctaaa
    32641 taatgggaca caaaccctta taccgacaga ttgctgatcg cattcgtgag caaattgcgc
    32701 gtggcgagct taaacctggc gatgcgttac ctaccgaatc ggcattacaa acagagtttg
    32761 gcgtcagccg ggttacggtg cgtcaggctt tgcgccagtt agtcgagcaa cagatcctcg
    32821 aaagcattca gggcagcggg acttacgtca aagaagagcg ggtcaattac gatatttttc
    32881 agttaaccag ttttgatgaa aaactgtcgg accgtcacgt cgatacacac agtgaagttc
    32941 tgatatttga agtgattccg gctgacgatt ttcttcagca acagctacaa atcaccgtgc
    33001 aggatcgtgt ttggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg
    33061 aagaaacctg gatgccgctt gcgttgttcc cggatctcac ctggcaggtc atggaaaatt
    33121 cgaaatatca ctttattgaa gaagtgaaga agatggttat cgatcgtagc gaacaggaaa
    33181 tcattcctct gatgccaacc gaagagatga gccgtctgct taatatcagc cagacaaagc
    33241 cgattcttga aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt gaatatagcc
    33301 gcaacgcttt taataccgat gactataaat ttaccctgat agcgcagcga aaatcatcgc
    33361 gataagcaca aaaaggccat catacgatgg ccaaccatgt cgaaaccgac agcccttcgc
    33421 cattcctgta gagggaagcg gcgagggcta tttcttatta cagatattta tttcagaaca
    33481 gttttcagtg cttcaccgat atccgccagg ctgcgaacgg ttttcacgcc tgcggcttcc
    33541 agagcagcga atttctcatc cgcagtccct ttcccaccgg caatgatcgc acccgcgtgg
    33601 cccatacgtt tgcctttcgg cgcagtcaca ccagcgatgt aacccacaac tggcttggta
    33661 acatgctctt tgatgtacgc tgccgcttct tcttcagcgc taccgccgat ctcaccgatc
    33721 atcacgatcg cttcggtctg cggatctttt tcgaacattt cgagaatgtc gataaagtta
    33781 gagccaggga tcgggtcacc gccgataccg acacaggtcg actgaccgaa accgtaatcc
    33841 gtggtctgtt taaccgcttc ataggtcagt gtaccggaac gggaaacgat acccacttta
    33901 cccggtttgt gaatgtgacc cggctggata ccaattttgc attcacccgg ggtgataacg
    33961 cctgggcagt tcgggccgat catacgaacg cctgcttcgt ccagtttcac tttcacggtc
    34021 agcatatcca gcgtcgggat gccttcagtg atggtgataa tcagtttgat gcctgcatcg
    34081 atagcttcca gaatggagtc tttgcagaac ggtgctggta cgtaaataac agacgcggaa
    34141 gcgccagtag cagcaacagc ttcacgcacg gtgttgaaca ccggcaggcc gaggtgggta
    34201 gtgccgcctt tacctggagt tacgccacca accattttag tgccgtatgc aatggcctgt
    34261 tctgagtgga aagtcccctg gctaccggta aagccctggc agataacctt ggtgttttta
    34321 tcaattaaaa tggacattat ttcccctcca ctgcggcaac aacctgctga gctgcatccg
    34381 tcagaccttt tgctgcaata atattcaggc cgctgtcagc cagtttcttc gcgccgagtt
    34441 cggcgttgtt accttccaga cgtaccacga ccggtacgtt aacacccact tccgctaccg
    34501 cgccgatgat accgtcagcg atcaggtcgc aacgaacgat accgccgaag atgttaacca
    34561 gaacggcttt cactttgtcg tcagagagga tgattttgaa cgcttcggtt acacgttctt
    34621 tggttgcgcc gccgccaacg tccaggaagt tagccggttc gccgccgtgc agtttaacta
    34681 tgtccatcgt acccatcgcc agacctgcgc cgttaaccat acaaccgatg ttaccgtcca
    34741 gcgcaacgta gttcagttcc cactgtgcag cctgtgcttc acgcggatct tcctgcgact
    34801 ggtcacgcat ttcgcgcaga tcaggctggc ggaacagggc gttgccatca gcgcccagtt
    34861 tgccgtcgag gcaaatcaga tcgccctgtt tggtgatgac cagcgggttg atttcgatca
    34921 gcgccaggtc gcgctccagg aaaatggtcg ccaggcccat gaagattttg gtgaactgct
    34981 gaaccagttt accttccaga cccagtttga acgccagctc gcgtccctga tacggcatcg
    35041 ggccagtcag cggatcaagc gcaactttat ggatcaggtg cggagtttct tccgccactt
    35101 tttcgatttc cacgccgcct tcggtggagg ccataaagac cacgcgacgg gaactacggt
    35161 ctacaacggc accgagatac agctctttag cgatatcggt cgccgcttca accagaatct
    35221 ggttaaccgg ttggccattg gcatctgttt gatacgttac cagacgcttg cccagccagt
    35281 tttctgcaaa agcacggatg tcttctttgc tgtttacaac tttcacaccg cccgctttac
    35341 cgcggccacc agcgtgaacc tgacatttca ctacccacgg accggcaccg atttttgaag
    35401 cggcttcttc tgcttcgcgc ggagtagtac aggcataacc caccggtgct ggtaagccat
    35461 agcgggcaaa cagttgtttt gcctgatatt catgtaagtt catgtgttct gtccatcctt
    35521 cagtaatcgt tatcttttaa accgtagacc gacaccctat ccacgtcggt ctacagtgca
    35581 ggtgaagctt aaactactac acgtccagca gcagacgcgt cggctcttcc agtaactctt
    35641 tgatcgttac caggaagccc acggattcgc gaccatcgat cagacggtga tcgtaggaca
    35701 gcgccaggta catcatcggc aggatctcaa cctgaccatt caccgccatc ggacgatctt
    35761 tgatagcgtg catacccaga attgcgctct gcggcgggtt gatgatcggc gtagacatca
    35821 gggaaccgaa cacaccaccg ttggtgatgg tgaagttacc accggtcaga tcttcaaccg
    35881 tcagcttgcc atcacggcct ttgactgcca gctctttgat tttcttctcg atgtctgcca
    35941 tgccgagggt atcgacatca cgcagaaccg gcgtcaccag gccgcgcggc gtagaaaccg
    36001 ccatgctgac gtcgaaatag ttgtggtaaa ccacgtcatc gccgtcgata gacgcgttca
    36061 cttccgggta acgtttcagg gcttcaacca ccgctttcac gtagaaggac ataaagccca
    36121 gacggatgcc gtggcgtttt tcaaacgctt caccgtactg cttacgcaga tccataatcg
    36181 gcttcatgtt gacttcgttg aacgtggtca gcatggcggt ggagtttttc gcttccagca
    36241 gacgctctgc cacacgctta cgcaggcgag tcatcgggac acgtttttca ctacgtgcag
    36301 ccagagccgg ttgcgccgcc ggagcagccg ctgccggagc agactctttc gccggggctt
    36361 tcgccagatg tttttccaca tcttcacgag tcagacgacc acccacaccg gtgcctttaa
    36421 tggcgctggc gtcgagattg tgttcagcca gcaggcgacg gatcgccggg cttaacgcat
    36481 cgttgttttg ctcttccaga gacgcctgct ggcgttgcgc cggagtggac gctttctctt
    36541 cagatttggc gctggtttct ttaccggcgc tgttgccttc acgcaggcga ccaaggatct
    36601 gacgagacgt taccgttgta ccttcatctt ccagaaccgc atccagaatg ccgtctgctg
    36661 atgccggtac ttccagtacc actttgtcag tttcgatttc taccagcact tcatcacgta
    36721 cgactgcgtc gccgggtttt ttatgccagg ttgcgacggt ggcatcggct acggattcag
    36781 gcaggtcagg gaccagaata tctacgctac tcattgtgta tcctttattt attcgacgtt
    36841 cagcgcgtca ttaaccagat cttgttgctg tttctggtga acggacatat accctaccgc
    36901 cggagaggcg gaggccgggc ggcctgcata acgcagagaa gccccaaacg gaatcacttc
    36961 acggaaatga tgctggctgc agtaccatgc gccctggttg agcggctctt cctggcacca
    37021 gacaaaatcc ttgacgtgag caaactgctg caacacttcc tgcatcgctt tatgcgggaa
    37081 cgggtagagt tgctcgatac gcacaatggc gacatcgtgt tgattgttct tacgacgctg
    37141 ttccagcagg tcgtaataaa ccttaccaga acacatcact acgcgcttca cgcccttcgg
    37201 atcaagctcg tcgatttcac cgatggctgg caggaaggtg ccgttcgcca gttcttcgag
    37261 gctggaaacc gccagcggat gacgcagcag ggatttcggc gacatcacga ccagcggacg
    37321 acgcatcccg cgcagcgcct gacgacgcag catgtggtaa acctgtgccg gggtagacgg
    37381 tacgcaaacc tgcatgtttt gctcagcaca aagttgcaga taacgttcca gacgcgcgga
    37441 ggagtgctcc ggcccctgcc cttcgtaacc gtgcggcagc aacatcacca gaccacacat
    37501 ccggccccat ttctgctcgc cagaggagat gaactggtcg ataaccacct gcgcaccgtt
    37561 ggcgaagtca ccgaactgcg cttcccagat ggtcagggtg cgtggttctg cggtggcata
    37621 accatattca aacgccagca cggcttcttc tgacagtacg gagtcccaga cacggaacgc
    37681 gccctgaccg ttatggatgt gttgcagcgg cgtgtaagtg gaaccgttag actggttgtg
    37741 gatcaccgcg tggcggtgga agaaggtacc gcgaccggag tcttcacccg acaggcgaac
    37801 cggaatgcct tcatcaacca gcgtggcgta agcgaggttt tccgcaccgc cccagtcgaa
    37861 cagtttctca ccggctgcca tcgcctggcg atcgccataa atcttggcaa cgcgagactg
    37921 catttcaact gcttctggta ccgtactgat acgtttagcc agttcctgca ggcgcttcat
    37981 ctcaactttg ttcgggtact cttcgtccca ttcgtggttg aggtacggcg accaggtgaa
    38041 agagtgcatg ttcatcggac gccactctgc cactacgcaa tcgccagcat ccagcgcatc
    38101 gcggtacagg ttaaccatct cggtggcatc ttccagcgtc gccacttttt cctgctccag
    38161 cttgtcagcg tagattttgc gcggtgtcgg atgttttttg attttctgat acatcagtgg
    38221 ctgggttgcg ctcggctcgt cggcttcgtt gtggccgtga cggcggtagc ataccaggtc
    38281 gatgaagaca tcacgtttaa aggtgttacg gaaatcgagc gccagacggg tcacaaaggc
    38341 aacggcttcc ggatcatccg cgttaacgtg gaaaatcggt gcctgaacca tcttaccgat
    38401 atcagtacag tacggtgtag aacgggcatc cagcgggtta gaggtggtga aaccaacctg
    38461 gttgttgata acgatacgta ccgtaccgcc aacttcataa ccacgcgctt tcgacatgtt
    38521 cagagtttcc tgaaccacgc cctgcccggt cactgcggcg tcaccgtgga tggtgattgg
    38581 cagcactttg ttgctgttcg gctcatcaag tctgtccaga cgggcacgaa cagagccgat
    38641 aacaaccggg cttacaatct caaggtgaga cgggttaaac gccagcgcca ggtgcaccag
    38701 gccgccatcg gtctggaagt cagacgagaa gcccatgtgg tatttcacgt cgcctgtgcc
    38761 gaggtgttct ttatgtttac cggcgaactc gtcgaacaag tcttgcggtt ttttacccag
    38821 cacgttcacc agcacgttca gacgaccacg gtgcgccatc ccgagaacca cttcgcgggt
    38881 gccgctgttg ccagcgtggc ggatcatctc tttaagcatc gggattaacg cgtcaccgcc
    38941 ttccagcgag aagcgttttg cgccagggaa ttttgcgccg aggtaacgct caaggccttc
    39001 agcggcggtc agttcgctta agaagcgttt tttctcttcg ctattgaaag tcgcgcgacc
    39061 agactcgata cgctgttgga tccagcgttt ttcttcggtg ctggtgatgt gcatatactc
    39121 ggcaccaatc gggccgcagt aggtttgctt gagggcggaa ataagctcgc cgagtttcat
    39181 ggtttctttg ccgctggcaa atgaaccgac gttgaaggtt tcctggaagt ctgcttcggt
    39241 cagatcgtgg aaagacggat ccagatcggc cactttatct tgctgccaca gtcccagcgg
    39301 atctagattc gcatgctggt gaccacggaa gcggtatgcg ttaatgagct gcaggacttt
    39361 aacctgcttc acattggtgt cagggtcgga gatcgttgaa gagtaacgtg aagcgtcttt
    39421 cgccaggcgg cggaaatatt cacgcgtttg agagtggaat tgatccggtt tgactcccgt
    39481 accaggtaac tgctggaacg tcgaacgcca gttagcgtca accgagtcag gatcggttaa
    39541 gaagtcttca tagagctgtt ctatccagct ctggtttgcg ccagagaggt aagaagagtc
    39601 caaccaggct ttcaaagcgc tgttctgcat cgtgatccct taagcatctt ttttatgctt
    39661 acttcgccgt ggatactacc acgcacagtg atgtgcgtgc cggggttcac ttgcgagctc
    39721 ttgtctacgt agtggctcgc gaaggaacct ttaaaaactg tcttagtgtc aggggcagtt
    39781 tttaaagatt tcctgcattt ctttgttacg cctgatgcgc tgcgcttatc aggcctgcgg
    39841 gtaatgactt gtaggccgga taaggcgttc acgccgcatc cggcactggt tgcctgatgc
    39901 aacgcttgcg cgtcttatca ggcctacagt ttacgcatta cgttgcaaca acatcgactt
    39961 gatatggccg atggcgcgcg tcgggttcag ccccttcgga catacactga cgcagttcat
    40021 gatgctgtga cagcggaata cgctgaatgc atcgctcaaa ccgtcgaggc ggctgtcagt
    40081 ctcggtatca cggctatcga tcaggaaacg atatgccgct aacaagcctg ccgggccgat
    40141 aaacttatcg ggattccacc agaaagacgg acaagaggtt gaacaacatg cgcagagaat
    40201 acattcatac agcccgtcga gtttttcgcg ctgctctggc atctgtaaat gctcgcgagc
    40261 tggcggattt tgtccattat tcaacaggta aggcttaatt ttctcatatt gcgcatagaa
    40321 ttgtcccatg tctaccacca aatcgcggat caccggtaaa cctggcagcg ggcgaatcac
    40381 aatcttcttg cccggctggt tgagtgccga aatcggggta atacaggcca gaccattctt
    40441 gccgttcatg ttcagaccgt cggaaccgca cacaccttca cggcaggagc ggcggaacga
    40501 caggctggga tctttctctt tcagctggat aagcgcatcc agcagcatca tgtcgcgacc
    40561 ttcttccgct tccagggtgt aatcctgcat acgcggagca tcatcaacat ccgggttata
    40621 gcgataaatt gaaaactcga gtctcatttt cctgtctccg cattagtaag tacgaatctt
    40681 cggcgggaat gccgggcgca gtttcggttc catgttgacg cttcggcgcg tcatggattc
    40741 cgactcaggc agatacaggg agtggcacag ccagttttca tcatcacgat ccgggaagtc
    40801 gaagcggcta tgtgcgccac ggctttcggt acggaagttg gcagaaacag ccgttgcata
    40861 cgccgtttcc atcaggttat ccagttccag gcactcaacg cgctgggtgt tgaactcgct
    40921 ggaagtgtca tccagacggg catttttcag acgctcgcgg atcactttca actgctcaag
    40981 ccctttcgcc atcgcatcac cttcacggaa gaccgagaag ttatgctgca tacattcttg
    41041 cagcgcttta cggatcgcca ccggatcttc accgttacga ttattgttcc accggttcag
    41101 gcgatccaga gacgcttcaa catcagactc gctggcatcg tgcagtgcgc cctgctcggc
    41161 gatagactct tgcagatgca gacctgccgc gcgaccaaag accaccaggt ccagcagcga
    41221 gttgccgccc agacggttag cgccgtgtac cgatacacaa gcgatttcac caacggcaaa
    41281 cagtcccgga acaaccacat cttcgccttt ctcattcaca gtcagcgcct gaccggtaac
    41341 tttggtcgga ataccgccca tcatgtagtg acaggttggg ataaccggaa tcggctcttt
    41401 caccggatcg acgtgagcga aggtacggga aagctccagg atacccggca gacgggattc
    41461 gagaacttct ttacccaggt gatcgagttt cagtttcgcg tgtggccccc acggaccatc
    41521 acagccgcga ccttcacgga tttcaatcat gatggaacgc gccaccacat cacggcccgc
    41581 caggtctttg gcgttcggcg cataacgctc cataaagcgt tcgccatgtt tgttcagcag
    41641 ataaccgcct tcaccacggc aaccttcggt gaccagtacg cccgcaccgg caatgccggt
    41701 cgggtggaat tgccacattt ccatatcctg caccggtacg cctgcgcgga tagccatgcc
    41761 gacaccgtcg ccagtgttaa tgtgggcgtt agtggtggac tgataaatac gccctgctcc
    41821 gccagtcgcc agcacggtag cgcgggcttt gaaataaacc acttcaccgg tttcgatgca
    41881 cagtgcggta caacccacca ccgcgccatc ctggtttttc accagatcca gcgcatacca
    41941 ctcggagaaa atggtggtgt ggtttttcag gttctgctga taaagcgtgt gcaacagtgc
    42001 gtgaccggta cggtcagcag ccgccgcagt gcgtgccgcc tgctcgccgc cgaagttttt
    42061 cgactgaccg ccaaacggac gttgatagat acgaccatca tcgagacgcg agaacggcag
    42121 gcccatgtgt tcgagttcca gaatcgcttc cggcccggtt ttacacatat attcaatcgc
    42181 gtcctggtca ccgatatagt ccgacccttt tacggtgtcg tacatatgcc attcccagtt
    42241 atcttcatgg gtattaccca gcgcaacggt aatgccgcct tgcgcagaaa cggtatggga
    42301 acgggtcggg aagactttag agagcagggc acaggtctgg ccgctctggg aaatttgcag
    42361 cgccgcgcgc atacctgcgc caccggcacc aatcacaact gcatcaaatt ctctgactgg
    42421 caatttcatc acacacccca caccacaacg aatccataaa tcacgtaaac caccagtgca
    42481 acgacaatca ccagttgcag catcaggcgc aaggccagcg gtttaacgta gtcggtcaac
    42541 acctgccaca tgccgatcca ggcatgaatc aagatagaaa acagcgccag cagggtgaac
    42601 actttggtga acgcagaggc gaagaaaccg atccagactt catatgtcag ttcgccactg
    42661 gtggcgaaaa aaccgaccat ataaatgatg tagagcgtca ggacgatagc ggtagcgcga
    42721 acgaggatga aatcatgtac gccattgcgt cctaatgcgg aggcgttgct taccatacga
    42781 ggactcctgc gagaagtgaa agcacgacag taataacaaa ggagattttg gcggagcgtt
    42841 tacccgcttc gaatgtttct tccagatagc caaaatccat catcatgtgg cgaataccta
    42901 cgacgacgtg atacgccaga gcggtaagga tgccccacat gataaatttg acgaagaagc
    42961 tgcccataat cgcggaagct tgctcgaaac cttcagggga agagaggctg gtacccagaa
    43021 gccactgcag gatgcccact gcaacaaagg tgatcacacc ggaaacgcga tggagaatgg
    43081 acgctatcgc cgtgatgggg aaccggatgg tctgtaggtc cagattaaca ggtctttgtt
    43141 ttttcacatt tcttatcatg aataacgccc acatgctgtt cttattattc cctggggact
    43201 acgggcacag aggttaactt tctgttacct ggagacgtcg ggatttcctt cctccggtct
    43261 gcttgcgggt cagacagcgt cctttccata actgcgcgtc atgcaaaaca ctgcttccag
    43321 atgcgaaaac gacacgttac aacgctgggt ggctcgggat tgcagggtgt tccggagacc
    43381 tggcggcagt ataggctgtt cacaaaatca ttacaattaa cctacatata gtttgtcggg
    43441 ttttatcctg aacagtgatc caggtcacga taacaacatt tatttaattt ttaatcatct
    43501 aatttgacaa tcattcaaca aagttgttac aaacattacc aggaaaagca tataatgcgt
    43561 aaaagttatg aagtcggtat ttcacctaag attaacttat gtaacagtgt ggaagtattg
    43621 accaattcat tcgggacagt tattagtggt agacaagttt aataattcgg attgctaagt
    43681 acttgattcg ccatttattc gtcatcaatg gattctttac ctgcaagcgc ccagagctct
    43741 gtacccaggt tttcccctct ttcacagagc ggcgagccaa ataacaaacg ggtaaagcca
    43801 ggttgatgtg cgaaggcaaa tttaagttcc ggcagtctta cgcaataagg cgctaaggag
    43861 accttaaatg gctgatacaa aagcaaaact caacctcaac ggggacacag ctgttgaact
    43921 ggatgtgctg aaaggcacgc tgggtcaaga tgttattgat atccgtactc tcggttcaaa
    43981 aggtgtgttc acctttgacc caggcttcac ttcaaccgca tcctgcgaat ctaaaattac
    44041 ttttattgat ggtgatgaag gtattttgct gcaccgcggt ttcccgatcg atcagctggc
    44101 gaccgattct aactacctag aagtttgtta catcctgctg aatggtgaaa aaccgactca
    44161 ggaacagtat gacgaattta aaactacggt gacccgtcat accatgatcc acgagcagat
    44221 tacccgtctg ttccacgctt tccgtcgcga ctcacatcca atggcagtca tgtgtggtat
    44281 taccggcgcg ctggcggcgt tctatcacga ctcgctggat gttaacaatc ctcgtcatcg
    44341 tgaaattgcc gcgttccgcc tgctgtcgaa aatgccgacc atggccgcga tgtgttacaa
    44401 gtattccatt ggtcagccat ttgtttatcc gcgcaacgat ctctcctatg ccggtaactt
    44461 cctgaatatg atgttctcca cgccgtgcga accgtatgaa gttaatccga ttctggaacg
    44521 tgctatggac cgtattctga tcctgcacgc tgaccatgaa cagaacgcct ctacctccac
    44581 cgtgcgtacc gctggctctt cgggggcgaa cccgtttgcc tgtatcgcag caggtattgc
    44641 ttcactgtgg ggacctgcgc acggtggtgc taacgaagcg gcgctgaaaa tgctggaaga
    44701 aattagctcc gttaaacaca ttccggaatt tgttcgtcgt gcgaaagata aaaatgattc
    44761 tttccgcctg atgggcttcg gtcaccgcgt gtacaaaaat tacgacccgc gcgccaccgt
    44821 aatgcgtgaa acctgccatg aagttctgaa agagctgggc accaaagatg acctgctgga
    44881 agtggctatg gagctggaaa acatcgcgct gaacgacccg tactttatcg agaagaaact
    44941 gtacccgaac gtcgatttct actctggtat catcctgaaa gcgatgggta ttccgtcttc
    45001 catgttcacc gtcattttcg caatggcacg taccgttggc tggatcgccc actggagcga
    45061 aatgcacagt tacggtatga agattgcccg tccgcgtcag ctgtatacag gatatgaaaa
    45121 acgcgacttt aaaagcgata tcaagcgtta atggttgatt gctaagttgt aaatatttta
    45181 acccgccgtt caaatggcgg gttaattttt atatgcctaa acacaaaaaa ttgtaaaaat
    45241 aaaatccact atcggacatt catatattct aaaaaaaaat acatcggtat aaattataaa
    45301 caaatagatg atttttaatc atctatcttc ctctcagtta catgaataaa ccaccaaata
    45361 tagcgcaccc ctataattcc caatacttta ttcatcaaac atttaattca taccatcaat
    45421 taattacttt tacatttaat aaaattaata atatttttaa gaaaaactaa aaacattcga
    45481 caatcaaata agatattctt gcaccgatat acttttttct tttctgttaa aggactcagt
    45541 tatgtttaaa ggacaaaaaa cattggccgc actggccgta tctctgctgt tcacagcacc
    45601 tgtttatgcg gctgatgaag gatccggtga aattcacttt aaaggtgaag ttattgaagc
    45661 accgtgtgaa atacatcagg atgatattga taaagaggtt gaactcggtc aggtgaccac
    45721 cagccacatt aatcagtcac atcacagcga tgccgttgct gtcgacctgc tcttagtcaa
    45781 ctgtgatctg gaaaactcca gcaacggttc cggtggcaag atttctaaag ttgcagtcac
    45841 ctttgatagc tcagcgaaaa ccaccggcgc agatccgatt ctcaacaaca ccagcacggg
    45901 tgaagccacc ggcgtcggcg tacgtttaat gaataaagat caaagcaata tcgttttagg
    45961 caccgctact ccagatatcg acctggctcc gacctccagc gaacaaacgc tgaatttctt
    46021 tgcctggatg gaacaaattg atcaggctac acctgtaacg ccaggcgcag ttacggcgaa
    46081 tgcaacctac gtgctggatt ataaataagt attactttgg cggcgctatc gccgcctttc
    46141 ataaaatatt tatcaggtat ggacaccgtg aatatttatc gactctcttt tgtatcctgc
    46201 ctggtcgtgg cgatgccttg cgcattggcg gtcgaattca atctgaatgt tctcgataaa
    46261 tcaatgcgcg accgcattga tatttcatta ttaaaggaaa aaggagtcat tgctcccggt
    46321 gagtattttg ttagcgttgc ggtaaataat aaccaaatca gtaacgagca aaagattaac
    46381 tggcacaaaa atgacgataa aaccattccg tgcatcaatg atttactggt cgataaattt
    46441 ggcttaaaac ctgaagtccg tcagtcgtta ccattgataa atcagtgcgt cgattttagc
    46501 tcccgacctg aaatgctctt caatttcgat caagccaatc agcaactgaa tattagtatt
    46561 ccgcaagcct ggctggcgtg gcactcagaa aactggaccc caccctccac atggaaagaa
    46621 ggtgtcgccg gtgtcctgat ggattacaac ttgtttgcca gcagctaccg cccacaggat
    46681 ggcagcagca gcactaatct taacgcctat ggaacagccg gaatcaacac gggggcatgg
    46741 cgcttacgca gtgattatca gttgaatcat actgatagcg atgataacca tgaacagtca
    46801 ggcgaaatat cacgtaccta tctttttcgc ccgttaccgc aattaggttc aaaattaacc
    46861 ctcggcgaaa cggattttag tcccaatatt ttcgacggtt tttcttatac cggcgcggca
    46921 ctggcaagtg acgatcgaat gttgccatgg gaactacgcg gctacgcccc acaaattagc
    46981 ggtattgcac agaccaatgc cacggtgacg atcagtcaat caggccgcgt catttaccag
    47041 aaaaaagtcc caccaggccc atttatcatt gacgacctta atcagtctgt tcagggcaca
    47101 ctggatgtca aagtgacgga agaagatggt cgggtgaaca atttccaggt ttcagcagca
    47161 tcgacgccct tcctgactcg tcagggacag gttcgctata aactggccgc gggtcagcca
    47221 cggccctcca tgtcacatca aactgaaaat gaaacctttt ttagcaatga agtttcctgg
    47281 gggatgctgt caaacacctc gctgtacagc ggcctgctgc tttctggtga tgactaccat
    47341 tctgccgcaa tgggtattgg gcaaaatatg ctgtggcttg gtgcgctgtc gtttgatgtc
    47401 acgtgggcca gtagccattt tgatactcag caggacgagc ggggcttaag ctaccgtttt
    47461 aattacagca aacaagtgga tgccactaac agcacgattt ccctcgccgc ttatcgtttc
    47521 tccgatcgtc attttcacag ctacgccaac tatctggatc acaaatacaa cgacagcgat
    47581 gcgcaggacg aaaaacagac gatcagctta tctgtgggcc aaccgattac cccactaaac
    47641 ctcaatcttt acgccaacct gctacatcaa acctggtgga atgcagacgc ctccacgacc
    47701 gccaacatca cggcaggttt taatgttgat attggtgact ggagagatat ctctatttct
    47761 acgtcattca atacaaccca ttacgaagat aaagatcgcg acaaccagat ttacctgtcg
    47821 atttcgctcc ccttcggtaa cggtggtcgg gttggctatg acatgcaaaa cagtagccac
    47881 agcaccacac accgcatgtc gtggaacgat acgctggatg aacgtaatag ctggggcatg
    47941 tctgccggac tgcaatccga ccgtcctgac aatggagccc aggtgagcgg taactatcag
    48001 cacctgagtt cagcgggtga gtgggatatt tctggtacct atgccgccaa tgattacagt
    48061 tccgtcagca gcagctggag cggttctttc accgcaaccc aatatggtgc agcgtttcat
    48121 cgccgcagct ccaccaatga accacgcctg atggtcagca ccgatggcgt ggcagatatt
    48181 ccggttcagg gcaatctcga ctacaccaac cattttggca ttgcggtggt gccgttgatt
    48241 tccagttacc agccttccac cgtggcggtg aacatgaatg acttacccga cggcgtaaca
    48301 gttgcagaaa acgttatcaa agaaacgtgg attgaaggcg cgataggtta caaatcactg
    48361 gcttcccgtt ccggtaaaga cgttaacgtc atcattcgca acgccagcgg tcagtttcct
    48421 cccctcggag cggatatccg ccaggatgac agcggcatta gcgtggggat ggttggcgag
    48481 gaaggacatg cctggttaag cggagtcgct gaaaatcaaa agtttaccgt ggtctggggt
    48541 gatagccagc attgctcgct ccatcttcct gaacatatgg aagacaccgc aaatcgcctg
    48601 attttacctt gtcattaatt aaggaaatga aaatgacatt tatgaaagga ctaccccttt
    48661 tgttgttggt tgccagtctg tgcagccacg ctgcactaca acccgatcgc actcgtattg
    48721 tgtttaacgc taatgataaa gctaccagtc tgcgggtgga taatcgtagc gataaactcc
    48781 cctacctcgc ttattcctgg ctcgaaaatg aaaaagggga gaaaagcgat gatcttctgg
    48841 tcgccctgcc gccgatacaa cgtctggaac cgaaagcaac aacacaggta cggatagtaa
    48901 agcaagcctc gaccacaaaa ttaccgggcg atcgcgaaac gctatttttc tacaatatgc
    48961 gtgaaattcc tcccgccccg gaaaaaaaca gcgaccatgc cgttctgcaa gtcgccattc
    49021 agagtcgtat caaggtgttc tggcgaccgg ctgcattacg caagaaagca ggagaaaagg
    49081 tcgaactgca gttacaggtc agccagcagg gcaaccaact gacactgaaa aatcctaccg
    49141 cgtattatct gaccattgcc tacctgggac gaaatgaaaa aggcgttctc cccggcttca
    49201 aaaccgtgat ggttgcgcct tttagtacgg tcaacacaaa caccggaaat tatagcggca
    49261 gccagtttta tctcggttat atggatgatt acggcgcgtt acgcatgacg acgctcaact
    49321 gcagcggaca atgccattta caagccgtgg aggcgaagaa atgagtgctg gcaagggagt
    49381 gttgctcgtc atctgtctgt tatttctgtc gttgaagtct gcgcttgccc tgaactgcta
    49441 ctttggttca tccggtggct cggtagaaaa atcagaagcg attcaacctt ttgctgtacc
    49501 aggcaatgcc aaactcggcg ataagatctg ggaatctgac gatattaaaa taagatctgg
    49561 gaatctgacg atattaaaat tcccgtctat tgtgacaaca ataccaacgg caactttgaa
    49621 agcgaacatg tctacgtatg ggtaaacccc tacccggggg tgcaggatcg ttattatcaa
    49681 ctcggagtaa cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac
    49741 actaaccagt gtatcgacag taagaacatt gacatttata cccctgatgg gatggcagaa
    49801 caaaatttgc tcgggtgatc ccgctaatat tcacatgtcg cgaacctttc tcgctcgcat
    49861 gcggttatac gtcaaaatac gagaaatgcc gccgcatgat tatcaaagta cgcttagcga
    49921 ctatatcgtc gtgcaatttg acggtgccgg tagcgttaac gaagacccta ctgcccaaaa
    49981 cctgaaatat catattactg gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt
    50041 ttccatttca ccggaaacac agatgattga ttttggtaaa tttaatttgc tggatattcg
    50101 tcgccacaca atgtcgaaaa cattcagtat taagacgaca aaatcacaaa atgaccaatg
    50161 caccgacgga tttaaagtca gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga
    50221 taaagcattg ttgattggta atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc
    50281 ttataccttc aataaatatg ctgaatacgc cgatttcacc agcgacatgt tggtctacga
    50341 aaaaacctat acagctgaac tttcgtccat tgcaggtacc cccatcgagg ctgggccctt
    50401 cgatacagtg gtgcttttta aaattaacta taactgattt gccatgatgc cctttgcagc
    50461 cggaaagggc atctctttca gaaaattgct gattttcccc ttttccttga ttgctgttat
    50521 tttttcctag cccggctttc tttatgctgt atcgatacac caaagagaaa ataatgaggg
    50581 agcgaaggat gccagttttg cagtggggaa tgttatgtgt gctatcactt ctcctttcta
    50641 ttggtttcct cgcggtacat ctcccggcgg cgctattact tgggccgatg atcgccggga
    50701 tcatcttcag tatgcgcgga attactctgc aacttccccg ctccgctttt ctcgccgcgc
    50761 aggccattct tggctgcatg attgcgcaaa acctcactgg ttcgattctc accacgctgg
    50821 ccgccaactg gccgatcgtg cttgctattt tactggtgac gctgctttcc agcgccatcg
    50881 tgggttggtt attggtgcgc tatagctcac tgcctggaaa taccggtgcc tggggttctt
    50941 ctccaggcgg cgcagcggca atggtcgcta tggcgcaaga ttacggcgca gatattcgcc
    51001 tggtggcgtt tatgcaatat ctgcgagtgc tgttcgtcgc gggggccgct gttctggtca
    51061 cccgcatgat gctgggtgat aacgctgaag cggttaacca gcaaatcgtc tggttcccgc
    51121 cagtgagcat taatctcctg cttacgattt tgctcgcagt cgttgccggt acggcgggat
    51181 gcatgctgcg tcttccttcc ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc
    51241 agtctggtca gctcatcacc atcgaactac cggaatggct gctggcgatg gcgtatatgg
    51301 caattggctg gcggattggt cttggtttcg ataaacaaat cttactgcgg gcactacgcc
    51361 cgctaccgca aatcctgctg tcgatttttg ctctgctggc tatttgtgca ggtatggcgt
    51421 ggggactgac ccggtttatg catattgatt ttatgactgc ctacctcgcc accagtcccg
    51481 gcgggcttga taccgtggcg gtcatcgccg cagggagcaa tgccgatatg gcgcttatta
    51541 tggcgatgca aaccctgcgc ctgttcagta ttttgctgac ggggccagcc attgcgcggt
    51601 ttatttcaac ctatgcgccg aagcggtcgg cctagtgctg gcagcctgga caccagtaaa
    51661 acgggcgcga ggacaacgtg gttttctcaa tgatgccgcc acagcgttcg cacagttcgc
    51721 catcacgatg aaaaacctta aagcgaaaca gcgccccatg atgcttattc tcatccacct
    51781 gcccccgcgt agcgtaagaa aaccgaggaa tctccagtaa cgcgtgtgcg agtgcatcca
    51841 gttgcgccgc attgagatct ttcgctttat gatttccagt caacccaacc tgccagagga
    51901 tctccacccg caaataattg ccaagcccag ccagaaacgc ctgatcgagc agtaatcctg
    51961 caaactgacg gttacgaaag cgcggcgaca ataaccgttc ctttaccacc tccggcgtca
    52021 gattcggatc cagcacatcg ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt
    52081 ccggcctcaa catctcaata tccgaggcgc tataaagcag aatggttttg tcagccgttt
    52141 gcagttttac ccgcaatact cgcgtggtct gcggctcttc gccggtatca accacgcgcc
    52201 agacgccgta aagctgatta tggctgtaga gcgttaaacc gttgggaaaa tgagttaaca
    52261 acgccttacc acgcgtttcc acatgggtaa cgtgttgacc gataagttgt gattgataag
    52321 tttttaactg cgggaaggca aaccagacat cagttagtgg tttgcctttg atcgccgcct
    52381 ccaggttatc cgctgcacgg cggatctccg ggccttcagg catggtgata tccttttttc
    52441 attctttatt aagcggggtt atgctgcgac aacaatcccc tttttggcaa atgtagctcg
    52501 taatcggcgg gcgaacgcca gagcgtgctc gccgtcgcca tgcaggcaga ccgtttgcgc
    52561 cgcgaccgtt gcccattcgc cggtgatact tttgactctg ccgtgttgca ccatctccag
    52621 cgtttgcgcc aacgcctgtt cttcgttttc aatcaacgcg cctggctgac ttcgcggcac
    52681 cagcgagccg tcagcctgat aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc
    52741 atattgcttg cctgcacgaa tcagctcgct tcctgccagc ccaacgagaa ccaatgccgg
    52801 atcgcaagcg tataccgctc tggcgatggc gtctgccagt tgtgcttctt tcgccgcctg
    52861 gttgtacaac atgccgtgcg gtttgacatg acgcattacg ccgccttgcg cacgggcaat
    52921 cgttgccagc gcgccaattt gatacagcgt ctgggcgtaa acggtttccg gcggcagctg
    52981 catggcgctg cgaccaaaat tttccctgtc gggaaaactc gggtgagcgc caatcgcgac
    53041 accatttttt attgcttcac gcacgcaagc ctgcatggtt tgcgcatcgc ctgcatgaaa
    53101 tccacaagca atattggcag aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc
    53161 ttcgcccaga tcggcgttca ggtcaatttt cattgtgcag ccgccacgct aattgttcaa
    53221 aataacgttg ctgatcttgc cgcgctttca gtgcctcttc cagtgaacac tggacaaaat
    53281 gaatcggctg accgagcgga atttgcgcca gatggtacat atcagcctca atgatacagg
    53341 caatacgtgg ataaccaccg gtggtctgtg cgtcgttcat caacacaatc ggctgcccgt
    53401 tatgcggcac ctgcaccacg cccggtaaca aaccgtgaga taacagttcg cgatcggtga
    53461 tgcgttttaa aatttgcccc tgtaaacgat agcccattcg gttactttgc gagctaagct
    53521 gccagggcga acgccagaat gcatcctgcg aggcgcgatc gaactcatga tattccggcc
    53581 ccggcaaggc gcgaatgcgg ttgccccaca gcagctgttt aacgccctgc gcttccataa
    53641 aatcacgctt cgatttgcca atcgggagtc agtcaccatc cttcagtaaa cggccttcca
    53701 gcccgccaat ccccactttg agatcggtgc tgcatgaccc cattaccggc ggaacatcaa
    53761 taccacccgc gaccgccaga taactgcgca tcccgtgctg cgggcgttta agcgttaaac
    53821 gctggcctgc tttcatcggc aatcgccagc cggtccagac ggcgttatca tccaaccgcg
    53881 cttcgcaacc tgcacccgtc agggcaaacc acccatcagt ttcgaactca actgttaact
    53941 gaccgagcgt gatctccagc gcgggggcat tggcgtcatt acccaccagc aggttagcaa
    54001 tgcgcagcgc aggcatatcc agtgcgccac agtggctgat acccgactgg caaaaaccgt
    54061 gacgaccgcc atcctgcaca gtggtgtaca tgcccgcacg aataatcttc agcatattcc
    54121 ctccttctgc ggtacaaatc gcacgctgtc tcccggacgt aataagatgg gttcgtcacg
    54181 cgccggatca aacaggctga gtgaggtatg accaatcaac tgccagccac ccggcgttgc
    54241 cagcggataa acaccagtct gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa
    54301 gcgcggttca gcgcgccgtg gcgtgtgtag ttgttccggc aaactcccga gatacgggaa
    54361 gcccggttga aaacctaaaa accagaccac gtattccacg gaggagtgca attcaacaac
    54421 ctgtttttcg ctcaacccgc aatacgccgc gaccaccgcc aaatccggtc cgcctgcacc
    54481 accgtaaacc accggaattt caataaagcg agactcaggt tccagggcct cgctctcctc
    54541 ccaccagcgt tgcaaacgct ctatggcatc cagcgccagc gactcaggat tacgcaaaat
    54601 caccgtgata ttgttcatgc cgggaatggc ttcaaccaca ttcggcatat ccaccagacg
    54661 ctgcgccagt cgccagatcc gtttctggct agccagcgtc accggcggtt ccagttccag
    54721 cactaccgcc gtttcaccta tcagataaca acgcgctcgt tgcactttcg atccctctga
    54781 ttattcgtta tgcaggatta ggaatatcaa taaaggtcac atcaagatcg gtattttcat
    54841 tcagccactc gctcaatgcg cgaataccac cgcgttcagt ggcatggtga cctgcagcat
    54901 aaaaatgcaa tccctgctcg cgggctgaat gaatggtctg ttcagaaact tcgccagtaa
    54961 taaaagcatc cacgccaaaa cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc
    55021 accaggcgac gcgctggacc acctcaggtc cggtatcgcc acaccataac ggcttacgtc
    55081 ccagacgcgc ttcaatccag gaggctagtt ccattcccgg cacaggcatg gtcagctctc
    55141 cccacggcac caacggctca atttcgccca tgaccgtgat ccccagtaac gccgccagct
    55201 gtgcgttatt gcccagctca ggatgtgcgt caagcggcaa atgccagcca tacaggttga
    55261 tatcattcgc cagcaacgtt tttaaacggt tacgcttcat tccgcgaatg accggagact
    55321 ctcctttcca gaagtagcca tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct
    55381 catcgagcaa agcctggctg gcggtgacac cggtaacaat tttttgcacc gtctctttgc
    55441 cttccacctg caaaccattc ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt
    55501 taatcagttg ttccagttcg gtgtttttca tcttcgcgcc ttccgtattc tgatagctac
    55561 accgttaaca tagcgttaca tcgcctgttc gtcgatagcc aatcttttga catattaaga
    55621 aacaaacccg acgccaaaag acgcaaattg tttatatcat cacgcttcag ctaatgtata
    55681 tttatgcaat cagacagcat attgcataaa taatcagatc tgtatcaaag ttttaacttt
    55741 aaggaaacac aagcacgccg cgcaactatt acgtgacgtt gccgtcaatt gtggaattat
    55801 tatgaataaa cacgcatcac aaccgcgcgc tatttactat gtcgttgcgc tgcaaatctg
    55861 ggaatatttt agcttttacg gcatgcgtgc cctgctgatt ctctatctca ccaatcaact
    55921 aaaatacaac gatactcacg cctacgagtt atttagcgcc tactgttcgc tggtgtatgt
    55981 cacgccaatc ctcggtggct ttttggcgga taaagttctc ggcaatcgca tggcggtgat
    56041 gctgggggcg ttgttgatgg cgatcggtca tgtggtgctg ggtgccagtg agatccatcc
    56101 gtcattcctc tatctgtccc tggcgattat cgtctgcggc tatggcctgt ttaaatctaa
    56161 cgtaagctgt ctgctcggcg agctgtatga gccaaccgat ccgcgtcgtg atggcggttt
    56221 ctcgctgatg tatgcggcgg gtaacgtggg gtctattatc gcacctatcg cctgtggtta
    56281 cgcccaggaa gagtacagct gggcgatggg ctttggcctg gcggcggttg gaatgatcgc
    56341 gggtctggtc attttcttat gtggcaatcg tcatttcact catacccgcg gcgttaacaa
    56401 aaaagtactg cgtgcgacaa actttctcct gccgaactgg ggatggctgc tggttctgct
    56461 ggtggcaacg cctgcactga ttaccgtgct gttctggaaa gagtggtcgg tatacgcctt
    56521 aattgtcgcg actattattg gcctgggtgt actggcaaaa atttatcgca aagcagaaaa
    56581 ccagaaacag cggaaggagc tggggctgat tgtgacgctc accttcttca gtatgttgtt
    56641 ctgggccttc gcacaacagg gcggtagctc gattagcctt tatatcgacc gcttcgttaa
    56701 ccgcgatatg tttggttata ccgttccgac cgcgatgttc cagtcgatta atgccttcgc
    56761 agttatgctg tgcggtgtgt tcctggcgtg ggtggttaaa gaaagcgtcg caggtaatcg
    56821 taccgtgcgc atctggggaa aatttgctct tggccttggc ctgatgagcg ccggattctg
    56881 cattctgacc ttaagcgccc gctggtccgc aatgtatggt cactcttctc tgccactgat
    56941 ggtgttaggc ctcgcggtga tgggctttgc ggaactgttt atcgacccgg ttgccatgtc
    57001 gcaaattacg cgcattgaaa ttccgggcgt gaccggcgta ttaaccggca tctacatgct
    57061 gctttctggc gcgatcgcga actatctggc gggcgtgatt gccgatcaga catcgcaggc
    57121 ttcgtttgat gcttccgggg cgatcaacta ctccatcaat gcatatattg aagtatttga
    57181 tcaaattacc tggggcgcac tggcgtgtgt aggactggta ctgatgattt ggctgtatca
    57241 ggcgctgaaa ttcagaaacc gcgcgctggc gctggagtca taataggcca gcaccgatgc
    57301 ctgatgcgac gcttgacgcg tcttatcagg cctacgtatt tcctgcaatt tattgaattt
    57361 gcacaaattt gtaggctgga ttaggcgttc acgccgcatc cggcatctgg gctcgattgc
    57421 ctgacgcgtc tgttatttcc ccttccgcgc cgcctcatac gctgccaacg tttgtactct
    57481 cgcctctttg tgctcgacta tcggttgcgg ataatccagc ttcacacctg ctttctgcgc
    57541 ccacttccac ggctcatgca ccgctttccc tggtacatcg cgcagctccg gtaaccatcg
    57601 acgaataaac tcgccctcac ggtcaaattt ctcgccctga gttatcggat tgaaaatacg
    57661 aaaatacggc gcagcatcag tacccgtaga agccgcccac tgccagccac cgttattagc
    57721 tgccaaatca ccatcaatca gctgcgacat gaaatatcgc tcgccttcgc gccagtcgat
    57781 caacaaatct ttcaccagaa aactggctgt aatcatccgt agccggttat gcatccagcc
    57841 agtgctgtta agctgacgca tagcggcatc aataatcggg tatcccgttt tgcctttctg
    57901 ccaggcctgt aaatgtgcgg gattgctttg ccactgcaca cgatccgtcc aggcaataaa
    57961 tggacgatgt ttacacaacg aggggtaata cgtcatcaga tgacggtaga actcgcgcca
    58021 gatcagctca ctaagccaga cactaccggc cccaccgtcc agcgcctgcg gctgttccgc
    58081 caacaagcga tgcaaacact ggcgaggcga taacccgccc gttgccaagc tggcggacaa
    58141 acggctggtg ccttccactg ccggaaaatc tcgttgttgc tcatactctc cagcaccgtt
    58201 ctcgcaaaac tggcgtaatt gcgcaatcgc cgctttttct tccaccggga aatgcgcagt
    58261 atcgaaagac tgacgaggat aattcagcgt aatggatggc gcgggcttta tcgatccgct
    58321 actacgaact tttggtgcag cgacgcactc cggcatccct tcccgcagcc gtttcagcca
    58381 ggcattctta aaaggcgtaa agactttgta catctcgtga ttgccggtca tcaccgcccc
    58441 cggcggcagg atcacgctgt catcaaatcc ttcacacacc acgttacgca gcgctctttc
    58501 aacgtccaca tcccgcgctc gctcattcac ctcatactga tagttataaa aaagatgagt
    58561 aacgctgttt tccgcgcaca cctgtttaac tatttcgaca ctggcgacaa agtcatccac
    58621 ttcacggaac aataaaggaa tacctttttc cgccagcgcg atttgtagcc cattcagttg
    58681 agcattgata agttcagcct gacgcggcga catgttatgc gtctcccact tgcgtggtgt
    58741 agcgatatac agcgccagca cgcgtgcaga cgaattgcgg caggcggcag ccagtgcgag
    58801 attatcgtgc agacgtaaat cctggcgaaa ccaggccaga tgggtagtca taaaactcct
    58861 gaatggcgca agttaatcac acgcggccaa agttcgaaat aacgctgccc tgaaagccag
    58921 gcgtcaggat aaagcgccat atagtgtttg atacgcatca gcggcgcaag aagtggttgc
    58981 tcgccacggc gataactgtc gataagcgca gccagctgct ggcgttctgt ggaatcaata
    59041 tgcgggcgaa aataaccctg aacgtgcatc aaaacgttgg tgtgatcgcg cgggttggcg
    59101 ggatgagaaa gcaaaacaat tacgcgttgg cggtactggt tgtaaaaatc atccagattt
    59161 tgccactgat gaatatcagc cacaaacggg ccaagtttgc ggtactcggg ctgagaatgg
    59221 gccagaaaga cgagtttata gcggctgtga taatcgagaa gcgccccgcg cgttaagcca
    59281 tgttctttca gtcgatgtaa ctcatgaagg gcgaagatac ggattagggt gctgtcatca
    59341 aatcgttgta gattcattac tgctcctgaa cggtatacag gagtaagtgt aggcgttggt
    59401 aaatgatgtt tgtgaagtta atccgctagc agggcaagat tacgaaagcg cgctccccgc
    59461 aagcaactaa tcaaggtagt taatcatggc tgaacacgat attacgccag aagtcactat
    59521 ttccaaaaca cagcgccgct ataaagtcgg ctatgtcaga gcccgccacc aggaccgcaa
    59581 aaccggtata gcccggtatt attcacaaca tcccagtctg caccttaagg gcgactggct
    59641 gaaagaggcc ggatttgata ccggacgggg tgtcacggtg aagatttcgg aggggggcct
    59701 gaccatcatc gctgacagtg acgaggtgca ggaactgcgg gatgggatgt ttagtgtgct
    59761 gaacgaaagt taaatattaa aaaagctggg aagatttaaa accttcctag ctttttattc
    59821 tgtcttatta taatggatac tcatcatctt ccgatgaatt tataaattgc tccaagttta
    59881 catgaagagt acttaaccta gtttttaact cattaagtga agcgataatt tcatattttc
    59941 caacataatc atatgatgga tcgtattcat tatctatgtc agaagctaaa aatgatgata
    60001 ttcgtagttt aaatttagct tcatctttat tacattcatt ggcataaggt gaatgcccta
    60061 tcatagcata ttcatctaat ctaaaaggat aagatggttt taattcatcg atcaatgaaa
    60121 tttcaatcca tgttctatat ccaggcatta atggactaat ttctttgagc aaaacctttc
    60181 cacttaataa tgtattgaga ttgttcatta ttatgtctcc cacgggaaaa atatatgtct
    60241 aacacttcca ccatgttgat gaacttgtaa atgtggtaaa tcaccatcag gatttcctgg
    60301 tccatgacct gacactctat atgaaggttt tgttacagaa ggatctttta tatcatccca
    60361 atgatatgaa cctgcttttg ttttattttc agtaagatat tccattaagt tcttagtgct
    60421 ggggcctgac tcacctgtca tatttttata attatgacct aaatatctat ccataaacaa
    60481 ttgttcagcg tcacttctag acctaacagt aactttggtt tcactactat ctaataaacg
    60541 attcgctttt tggttactaa cagcacatgg agttaatcca agtggatcag catattttag
    60601 aggattaggt gcataactgt agagatttaa accgcctctc aaccctatcg ggtcagttac
    60661 catgaaccgc ccgacatccg gatcatagta cctgtgcaga ttgtagtgca gccccgtttc
    60721 cctgtcgagg tactgtccct ggtagcgcag gttctgctcc accccgccgt ggtccttctc
    60781 ctgtatcgtg ttaccccaga cctgataccc cgtctcccac acgattttcc cgtcgctgtc
    60841 cgtcatctct tccggcgcgc cgttcacatc cgtgtgaaaa tacagtatcc ggttcgggcc
    60901 ctctttgccc gccttatcca cccgcgccag cggttcataa ctcccctggt ccctgtagat
    60961 gtacaggctg cctgttccct ccggcctgct ctcctgcgcc agccgcattc cgtcccataa
    61021 aaattttgtc cggttgtacg gcttaccgtc agggctgatg tgctgttttt ccgtccgcct
    61081 ccccagtgca tcgtacacat accggtatgt cccccgcgct gtcgtcactt ccaccatccg
    61141 gtgttccgca tcataacggt aactctgcgt tccgctccgg ccctcctttt ccaccgcacg
    61201 gccaaattcg tcataacggt agtgcacacc acggtaagac atcagccggt tgaacggcac
    61261 cacgctgttt tctgccgtgg ccctttcccc cgcgcgcctc cccagcaggt ttcccgccgc
    61321 gtccacctcc gtttccggct ggtgccacac cgggtgccac tgcgcctgct ccacccgccc
    61381 cagcgggtcg tgcgtctccg tgatatgccc ctgcaggtca tacacatagc gccatttgcc
    61441 gccctgaggg tcggtcatcc ccagcaacag tcgttcttcg tcgctccagc ggaatgtcgt
    61501 cacctgtccg gcttcgtcct ggtacgccgt cacctgattc tgcgcatccc actggtgata
    61561 acgtgtgcgc cccagtccgt ccgtcacgca cagcctgcgc tgcgccaggt cccacgccag
    61621 ccggtactgt tcaccgctgt ccgtgtggtg ccccgtcacc cgccatgccg cgtcatccgg
    61681 accttcatac cagccccact ggtaataata cccttcgccg cccggctggc ggtgcagcgt
    61741 catgcagtgg ttttcgtcgt aagcaaactc acgcagcagg tgccccgtgc tgtcgtacac
    61801 gccgttcagg tggccggcct cgtcatagct gtaacgacgc agcagtaaag gtgcagtctc
    61861 agggaaatgc tgataaatct gtgtcacccg ccggggatgc gccgccagtt cgtaatacag
    61921 acggatgcac ggacgctgct gttcgccgct gatttccaca atgcggcccc ggtcgtcgta
    61981 gtacagattc agacagttgc tgttacggtc gcccagcatt ttcagccgct ggcgctgcgg
    62041 gtggtgcggg tcagcctcaa acagaaaaca ctgcccgtca tcgctgctga taagccagtg
    62101 cccctgctca ccacgcctta ccgccagccc ttcaccgggg ctgtaaaacc cgccccccgg
    62161 cagtacggct tccatatcca agcgtcgccc catgcccgac acaaaggtca tgcagttttc
    62221 gtctgcgcta cccggtgtgc acacgaggct gacttcatac attacactcc agccctgacc
    62281 aaacattcct tccgtacgtt cgtcacgact gctgtacacg cgctgccagt ccagcggaaa
    62341 gtggcccggc agactgaaat cgaagtcatc gtcatcggcc aggtatttgg ccccggtggc
    62401 ggcattgacc ggatgcggaa gggcactggt ggccttctgc gtcagcatgc tcatcccgac
    62461 actggcagca aaacaggcga ttttagaaag cattttgccc gggcgaagaa acatcagcgc
    62521 cacggttgcc accagggtta tctgcgacct gccgcttctg atatccctga ccaccagtaa
    62581 cggaccgcca atgcgcacat ccggtgacac aaacgccccg ttccccggct cattcaccac
    62641 cctggcctcg caggtacagc gtgccccgct gcgtgtcgcc ggctggctgt tgatacgcac
    62701 atcccgcgcg ccttccgcga tatattccgc tgcgggcggc tcgtcatcgc ctttgaacag
    62761 attactgatg ctgccaataa ccgcattcac tccctgaaag gcggtttcca gcataccgcc
    62821 cggattcagc aggttaaggg ccgttcccag ggggtcatcc agaaaggcgg cttttttttg
    62881 tgcgagaaac tgcgtcagtg aggaaggatg tttatggcag tcgattttat cgtcaggggt
    62941 cggggtggat cccggggccg cactggccac agtgggctgc catagttccg acacaaaatg
    63001 tgtcacgcca tccaccattt cagaacgccc ttcttccgta ctgagctggt cgtgcagagc
    63061 ccgggacatc agcacaggcc ctgtcaccgg gttaaccgcc agggctatgg cccccgccat
    63121 ttccagatat tccccggcca catcccggaa cgttctggca tcctcctgac gctgcgcttc
    63181 ttcctgttct gccttcgcct gcgcttcctg cgcctctatg tcctgccgcg atgccgcagt
    63241 cgccgctgcc cgcgcggcag gtttgctgtt gatccatgtg tcaccagagc cggtgaggat
    63301 gtaaccttca atctgaggcg ggaaaagcat atttgcgagg gctgaacagg cttcgctgac
    63361 cttttctgtc acgccggtgt acaccatcgc cacacccgcc acaaaaccgg tcagcacgac
    63421 ggccgtgcac cccatcgtga tccccactga cgtcgccagt aacgaggttg tgccgacaat
    63481 gagggcatca ataacgacat ttgcggccac ctccagcacc gcccccagca catctgccat
    63541 aaacgacgta tgcagcaggg catcaccctg tcttgccgcc cagaatgcct cttccatgtc
    63601 ccgttccctg tatgattaat tcgtcctgac ttcctgccgc atcctgaagc gcagattctt
    63661 tttaatttca gcccagcgcg tcatatctgc cggggtcagc ggtgtggttc tggcgtaggt
    63721 gaaggccatc atgacccgct cacccggcaa tacaaatgcc acctgacact ggtacacctg
    63781 ctcgttaccc cggagaaact ggctctgtat ttccaaaccg cctcgttgag ataatattcc
    63841 gtcataatgt aaatagtccg ttttaataaa ggaattattt attgacaggt tctgtttttt
    63901 taatcatgaa tgtaataccc atcaccgctt tacagcacac tttatctttc cagtcataat
    63961 cactaataat attaagcaat tagcaatatt tacaacgacc agagcggata catagttatg
    64021 tatttaacaa tcccaatata ttcactatgc aaataaaacc actagtaaca gacacgctac
    64081 cgcctccata taaaaatgga caccttataa aattgtatct tgcatagaaa aagctgaaat
    64141 atcggatgcg ttttaattat caactataaa tctataagct ttttattaac aac
//
LOCUS       DAJTRD010000012        60577 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTRD010000012 DAJTRD010000000
VERSION     DAJTRD010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60577
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.012"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            535..1824
                     /gene="sdaC"
                     /locus_tag="OO315_000839"
     CDS             535..1824
                     /gene="sdaC"
                     /locus_tag="OO315_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5889856.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            1882..3249
                     /gene="sdaB"
                     /locus_tag="OO315_000840"
     CDS             1882..3249
                     /gene="sdaB"
                     /locus_tag="OO315_000840"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5889857.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            3360..4115
                     /gene="xni"
                     /locus_tag="OO315_000841"
     CDS             3360..4115
                     /gene="xni"
                     /locus_tag="OO315_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5889858.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(4168..5316)
                     /gene="fucO"
                     /locus_tag="OO315_000842"
     CDS             complement(4168..5316)
                     /gene="fucO"
                     /locus_tag="OO315_000842"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5889859.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(5344..5991)
                     /gene="fucA"
                     /locus_tag="OO315_000843"
     CDS             complement(5344..5991)
                     /gene="fucA"
                     /locus_tag="OO315_000843"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5889860.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            6538..7854
                     /gene="fucP"
                     /locus_tag="OO315_000844"
     CDS             6538..7854
                     /gene="fucP"
                     /locus_tag="OO315_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5889861.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            7887..9662
                     /gene="fucI"
                     /locus_tag="OO315_000845"
     CDS             7887..9662
                     /gene="fucI"
                     /locus_tag="OO315_000845"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5889862.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEASACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            9771..11190
                     /gene="fucK"
                     /locus_tag="OO315_000846"
                     /pseudo
     CDS             9771..11190
                     /gene="fucK"
                     /locus_tag="OO315_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            11192..11614
                     /gene="fucU"
                     /locus_tag="OO315_000847"
     CDS             11192..11614
                     /gene="fucU"
                     /locus_tag="OO315_000847"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5889863.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            11672..12403
                     /gene="fucR"
                     /locus_tag="OO315_000848"
     CDS             11672..12403
                     /gene="fucR"
                     /locus_tag="OO315_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5889864.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(12447..13547)
                     /gene="rlmM"
                     /locus_tag="OO315_000849"
     CDS             complement(12447..13547)
                     /gene="rlmM"
                     /locus_tag="OO315_000849"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5889865.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(13540..13935)
                     /locus_tag="OO315_000850"
     CDS             complement(13540..13935)
                     /locus_tag="OO315_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5889866.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(13954..14871)
                     /gene="gcvA"
                     /locus_tag="OO315_000851"
     CDS             complement(13954..14871)
                     /gene="gcvA"
                     /locus_tag="OO315_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5889867.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(15222..15449)
                     /locus_tag="OO315_000852"
     CDS             complement(15222..15449)
                     /locus_tag="OO315_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5889868.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            15641..16846
                     /gene="csdA"
                     /locus_tag="OO315_000853"
     CDS             15641..16846
                     /gene="csdA"
                     /locus_tag="OO315_000853"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5889869.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            16846..17289
                     /gene="csdE"
                     /locus_tag="OO315_000854"
     CDS             16846..17289
                     /gene="csdE"
                     /locus_tag="OO315_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5889870.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(17340..18146)
                     /gene="tcdA"
                     /locus_tag="OO315_000855"
     CDS             complement(17340..18146)
                     /gene="tcdA"
                     /locus_tag="OO315_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5889871.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(18384..19481)
                     /gene="mltA"
                     /locus_tag="OO315_000856"
     CDS             complement(18384..19481)
                     /gene="mltA"
                     /locus_tag="OO315_000856"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5889872.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFVEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            19690..19766
                     /locus_tag="OO315_000857"
     tRNA            19690..19766
                     /locus_tag="OO315_000857"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19724..19726,aa:Met,seq:cat)
     gene            19800..19876
                     /locus_tag="OO315_000858"
     tRNA            19800..19876
                     /locus_tag="OO315_000858"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19834..19836,aa:Met,seq:cat)
     gene            complement(19951..21204)
                     /gene="amiC"
                     /locus_tag="OO315_000859"
     CDS             complement(19951..21204)
                     /gene="amiC"
                     /locus_tag="OO315_000859"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5889873.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            21436..22767
                     /gene="argA"
                     /locus_tag="OO315_000860"
     CDS             21436..22767
                     /gene="argA"
                     /locus_tag="OO315_000860"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5889874.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(22829..24655)
                     /gene="recD"
                     /locus_tag="OO315_000861"
     CDS             complement(22829..24655)
                     /gene="recD"
                     /locus_tag="OO315_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5889875.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(24655..28197)
                     /gene="recB"
                     /locus_tag="OO315_000862"
     CDS             complement(24655..28197)
                     /gene="recB"
                     /locus_tag="OO315_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5889876.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(28190..31078)
                     /gene="ptrA"
                     /locus_tag="OO315_000863"
     CDS             complement(28190..31078)
                     /gene="ptrA"
                     /locus_tag="OO315_000863"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5889877.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(31254..34622)
                     /gene="recC"
                     /locus_tag="OO315_000864"
     CDS             complement(31254..34622)
                     /gene="recC"
                     /locus_tag="OO315_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5889878.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(34635..34985)
                     /locus_tag="OO315_000865"
     CDS             complement(34635..34985)
                     /locus_tag="OO315_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5889879.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(34970..35377)
                     /locus_tag="OO315_000866"
     CDS             complement(34970..35377)
                     /locus_tag="OO315_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5889880.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(35374..35937)
                     /locus_tag="OO315_000867"
     CDS             complement(35374..35937)
                     /locus_tag="OO315_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5889881.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(35928..36398)
                     /locus_tag="OO315_000868"
                     /pseudo
     CDS             complement(35928..36398)
                     /locus_tag="OO315_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(36582..37376)
                     /gene="thyA"
                     /locus_tag="OO315_000869"
     CDS             complement(36582..37376)
                     /gene="thyA"
                     /locus_tag="OO315_000869"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5889882.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(37383..38258)
                     /gene="lgt"
                     /locus_tag="OO315_000870"
     CDS             complement(37383..38258)
                     /gene="lgt"
                     /locus_tag="OO315_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5889883.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(38409..40655)
                     /gene="ptsP"
                     /locus_tag="OO315_000871"
     CDS             complement(38409..40655)
                     /gene="ptsP"
                     /locus_tag="OO315_000871"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5889884.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(40668..41198)
                     /gene="rppH"
                     /locus_tag="OO315_000872"
     CDS             complement(40668..41198)
                     /gene="rppH"
                     /locus_tag="OO315_000872"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5889885.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            41883..42572
                     /gene="mutH"
                     /locus_tag="OO315_000873"
     CDS             41883..42572
                     /gene="mutH"
                     /locus_tag="OO315_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5889886.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            42641..43354
                     /locus_tag="OO315_000874"
     CDS             42641..43354
                     /locus_tag="OO315_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5889887.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            43492..43710
                     /gene="ygdR"
                     /locus_tag="OO315_000875"
     CDS             43492..43710
                     /gene="ygdR"
                     /locus_tag="OO315_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5889888.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            43818..44858
                     /locus_tag="OO315_000876"
     CDS             43818..44858
                     /locus_tag="OO315_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5889889.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(44891..46081)
                     /gene="lplT"
                     /locus_tag="OO315_000877"
     CDS             complement(44891..46081)
                     /gene="lplT"
                     /locus_tag="OO315_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5889890.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(46074..48233)
                     /gene="aas"
                     /locus_tag="OO315_000878"
     CDS             complement(46074..48233)
                     /gene="aas"
                     /locus_tag="OO315_000878"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5889891.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            48819..49850
                     /gene="galR"
                     /locus_tag="OO315_000879"
     CDS             48819..49850
                     /gene="galR"
                     /locus_tag="OO315_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5889892.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(49857..51119)
                     /gene="lysA"
                     /locus_tag="OO315_000880"
     CDS             complement(49857..51119)
                     /gene="lysA"
                     /locus_tag="OO315_000880"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5889893.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            51241..52176
                     /gene="lysR"
                     /locus_tag="OO315_000881"
     CDS             51241..52176
                     /gene="lysR"
                     /locus_tag="OO315_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5889894.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(52163..52855)
                     /gene="ygeA"
                     /locus_tag="OO315_000882"
     CDS             complement(52163..52855)
                     /gene="ygeA"
                     /locus_tag="OO315_000882"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5889895.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(52984..54402)
                     /gene="araE"
                     /locus_tag="OO315_000883"
     CDS             complement(52984..54402)
                     /gene="araE"
                     /locus_tag="OO315_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5889896.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(54717..55478)
                     /gene="kduD"
                     /locus_tag="OO315_000884"
     CDS             complement(54717..55478)
                     /gene="kduD"
                     /locus_tag="OO315_000884"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5889897.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(55508..56344)
                     /gene="kduI"
                     /locus_tag="OO315_000885"
     CDS             complement(55508..56344)
                     /gene="kduI"
                     /locus_tag="OO315_000885"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5889898.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(56631..57812)
                     /locus_tag="OO315_000886"
     CDS             complement(56631..57812)
                     /locus_tag="OO315_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5889899.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            58067..59296
                     /locus_tag="OO315_000887"
                     /pseudo
     CDS             58067..59296
                     /locus_tag="OO315_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
     gene            59931..60577
                     /locus_tag="OO315_000888"
                     /pseudo
     CDS             59931..60577
                     /locus_tag="OO315_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT        14969 a        16262 c        14764 g        14582 t
ORIGIN      
        1 tgttgccggg ttttctcgtt tttggtcact tactcatcaa ctcatttcat ttgttatatg
       61 aatgtttctt accaccctca cgcgacaaat atcatcacag ttaatatgtc atacaattta
      121 tgttgcaaca caaacgtttc cctattttca taaaccgtat tttatcgcta tgaaaaagaa
      181 attatcgcca tgattaacta aaagtattga ttttttcagt tcaacctaca tatattgcgc
      241 gccccggaag aagtcagatg tcgtttaatg ggcaaatatt gcccttaaat tctcttttac
      301 ttttgattta cagagtaaag cgttgggata atctatcttc caagcagatt attgtatttg
      361 ggatcaagat cactgataga tacataactt gtgtgtatct ttccgccctc aaattattac
      421 ggcggtaaat gattaagcca tcgccgatag acagatttca tttttacggt caggcacctt
      481 cccgggctga actggctaaa agctgaatta tttgcattcc tccaggagaa atagatggaa
      541 acgactcaaa ccagcacgat tgcgtcgaaa gactctcgta gtgcctggcg caagacagac
      601 accatgtgga tgctgggcct ttacggcacg gcaatcggcg cgggcgtgct gttcctgcca
      661 atcaacgccg gtgttggcgg tatgatcccg ctgatcatca tggctatcct tgcgttcccg
      721 atgacgtttt ttgctcaccg cggcctgact cgcttcgtac tgtctggtaa aaacccgggc
      781 gaagacatca ccgaggttgt agaagaacac tttggtattg gcgcaggtaa actgattacc
      841 ctgctctact tcttcgctat ctacccgatc ctgctggttt atagcgtggc aatcaccaat
      901 accgttgaaa gcttcatgtc tcaccagctg ggtatgacgc caccgccgcg tgcgattctg
      961 tcgctgatcc tgatcgtggg tatgatgacc atcgttcgct ttggtgagca gatgatcgtt
     1021 aaagcgatga gtattctggt attcccgttt gttggcgtac tgatgctgct ggctctgtac
     1081 ctgatcccgc agtggaacgg cgcagcactg gaaacgctgt ctctggacac tgcatctgca
     1141 accggaaacg gtctgtggat gaccctgtgg ctggcaattc cggtaatggt gttctcgttc
     1201 aaccactctc cgatcatctc ttctttcgcc gttgcgaagc gtgaagagta cggcgatatg
     1261 gcagaacaga aatgctcgaa gatcctggca ttcgcacaca tcatgatggt gctgaccgta
     1321 atgttcttcg tcttcagctg cgtactgagc ctgactccgg cagacctggc tgcggctaaa
     1381 gagcagaaca tctcgattct gtcttacctg gctaaccact ttaacgcacc ggttatcgcg
     1441 tggatggctc cgattatcgc gattatcgct atcaccaaat ccttcctcgg ccactacctg
     1501 ggcgcacgtg aaggcttcaa cggtatggtg attaaatctc tgcgtggtaa aggtaagtct
     1561 atcgaaatca acaagctgaa ccgtatcact gcgctgttca tgctggtaac gacctggatt
     1621 gttgccaccc tgaacccgag catcctgggt atgattgaaa ccctgggcgg cccaatcatc
     1681 gcgatgatcc tgttcctgat gccgatgtac gcaattcaga aagtaccggc aatgcgtaag
     1741 tacagcggtc acatcagcaa cgtattcgtt gtcgtgatgg gtctgattgc aatctccgca
     1801 atcttctact ctctgttcag ctaagtcctt tcgcgccgct ttcgggcggc gcttcctccg
     1861 ttttaacgcg atgtatttcc tatgattagc gtattcgata ttttcaaaat cggcattggc
     1921 ccttccagtt ctcataccgt tggaccaatg aaagcgggta aacaatttac cgacgatctg
     1981 attgcccgta acctgcttaa agacgtgacc cgcgtggtgg ttgacgtgta cggctcgctc
     2041 tctctgactg gtaaaggcca ccacacagat atcgccatta ttatgggcct ggcgggtaac
     2101 ctgccggata ccgtggatat cgattccatc cccggtttta ttcaggatgt gaatactcat
     2161 ggtcgcctga tgctggcaaa cggtcagcat gaagtggagt tcccggttga tcagtgcatg
     2221 aacttccatg cagacaacct ttctctgcat gaaaacggta tgcgcattac cgcgctggcg
     2281 ggcgataaag tcgtttacag ccagacttac tactctattg gcggtggctt tatcgttgat
     2341 gaagagcatt ttggccagca ggatagcgca ccggttgaag ttccttatcc gtacagttca
     2401 gcagccgatc tgcaaaaaca ttgtcaggaa accgggctgt cactctctgg cctgatgatg
     2461 aaaaacgagc tggcgctgca cagtaaagaa gagctggaac agcacctggc gaacgtctgg
     2521 gaagtcatgc gtggcggtat tgagcgcggt atttccaccg aaggcgtgtt gcctggcaaa
     2581 ctgcgcgttc cacgccgtgc tgcggcacta cgccggatgc tggtcagcca ggataaaacc
     2641 accactgacc cgatggcggt tgttgactgg atcaacatgt ttgcactggc agtgaacgaa
     2701 gagaacgctg ctggcggtcg cgtggtgact gcgccgacta acggtgcgtg cgggattatc
     2761 ccggctgtgc tggcgtacta cgataagttt atccgcgaag tgaacgctaa ctcactggct
     2821 cgttacctgc tggtagccag cgccattggt tctctttata agatgaacgc gtcgatttct
     2881 ggtgccgaag tgggttgcca gggtgaagtt ggcgtggcgt gctctatggc ggcagctggt
     2941 ctggcagaac tgttaggcgc aagcccggcg caggtttgca tcgcagcgga aatcgcgatg
     3001 gagcacaacc tcggtctgac ttgtgacccg gtcgcggggc aggttcaggt accgtgcatt
     3061 gagcgtaacg ccattgcagc agtgaaagcg gtgaacgccg cgcgtatggc actgcgccgt
     3121 accagcgaac cgcgcgtctg cctcgataaa gttatcgaaa ccatgtacga aacaggtaaa
     3181 gacatgaacg ccaagtaccg cgaaacctct cgcggcggcc tggcgatgaa gatcgttgcc
     3241 tgcgattaat ctctcccaga ggcctcgttt tgcgaagcct cttcctgttt tctcaaccag
     3301 ccatagcctg ttccggcatc gaatgttacc cttatcgcct gatctttaag ggggttatcg
     3361 tggctgttca tttgcttatt gtcgatgcac tgaatcttat ccgtcgcatt catgccgttc
     3421 agggttcgcc ctgtgtcgaa acctgccagc atgcgctcga tcagctcatt atgcacagcc
     3481 agccaaccca cgcggtcgcc gtttttgatg atgaaaaccg cagtagcggc tggcgtcatc
     3541 agcgtttacc agattacaaa gcggatcgac cgccaatgcc ggaagagttg cacgacgaga
     3601 tgcctgcatt acgcgccgcc tttgagcaac gcggcgtccc gtgctggtca gccagcggca
     3661 acgaagccga tgacttagcc gccacactgg cggtcaaagt gacacaggcc gggcatcagg
     3721 caacgattgt ttcgacagat aaaggctact gtcagttact ttcaccgaca ttacgtattc
     3781 gtgattactt ccagaaacgt tggctggatg cgccatttat cgataaagaa tttggcgttc
     3841 aaccgcagca gttgcccgat tactggggac ttgcggggat cagcagttca aaggtaccgg
     3901 gtgttgcggg aatcggacca aaaagcgcca cgcagctgct ggtcgagttt cagagtctgg
     3961 aagggatata tgagaatctg gatgcggttg ccgaaaagtg gcgcaaaaaa ttagaaaccc
     4021 ataaagagat ggcgtttctg tgccgcgata ttgcccgctt acaaaccgat ttgcatatcg
     4081 acggcaattt acagcaattg cggttggtac ggtaacggcg agccggatac gccgcaaatc
     4141 gtatccggca ttatcacatc agcgcattta ccaggcggta tggtaaagct ctacaatatc
     4201 ctcaagcgtt gcttcacgcg ggttgccacc ggtacaaaca tcatccagtg ccgcctgagc
     4261 cagtgccgaa atgtcttcct tgcgtacacc aacatcacgc aaatgtggcg gaataccgac
     4321 atcacggttg agggcaaaca ccgcttcaac agcggcatta cgcgcccctt ccaggctcat
     4381 accttccact ttcacgccca taacgcgcgc gatatcgcgg tacttctcac cggtaaagtc
     4441 agcgttatag cgcatgacat gcggtaacag gatggcgttc gcaacaccgt gtggagtgtt
     4501 ataaaacgcg cccagtggat gcgccatacc atgcaccaac cctaacccaa cattcgagaa
     4561 gcccataccc gcaacatact gcccgagcgc catttcttct ccggcatcct tatcaccagc
     4621 aaccgatcct cgcagcgccc cagcaatgat ttcaatcgct ttaatgtgca gtgcatcggt
     4681 tagtgcccac gcgccacggg taatataccc ctcaatagca tgagtgagcg catcaacacc
     4741 cgtcgcggct ttcagcgccg gaggcatacc atccatcatg tcagcatcaa taaacgccac
     4801 ctgcgggata tcatgcggat caacgcaaac aaacttgcgc cgtttttctt cgtcagtgat
     4861 cacgtagtta atggtcactt ctgccgcagt acctgctgtg gtggggattg ccagaatcgg
     4921 tacactgggt ttattggtcg gggaaagccc ttccaggcta cgcacatcgg caaactccgg
     4981 gttgttgctg ataatgccaa ttgctttaca agtatcctgt ggagaaccac caccaatagc
     5041 gatcaggtaa tccgcgccgc tattctggaa tacaccgagc ccttctttga cgacagtaat
     5101 tgttgggttg ggcactacgc cgtcgtaaat cgcccatgcc agccctgcag catccatctt
     5161 atcggtcact ttcgccacca cgccgcattg caccagcgtt ttatcggtga cgatcagcgc
     5221 cttctgataa ccacggcgtt tcacctcatc ggttaaagcc ccaacagcac cccgaccaaa
     5281 ccatgccgtt tcgttcagaa tcattctgtt agccatcatc cttctccttg ttgctttacg
     5341 aaattactct tcaatccgta acccataggt tttgaatttc tccagcacta cggcaatctc
     5401 ttcatcgctc agcactggca ccgggtccgt aatcgccagg gtcgtcaggt aaagttgcgc
     5461 cagcacttca acttcatgcg ccagccataa cgctttttcc agattcacct cacaagcgat
     5521 aagcccatga tgttgtaaca aagttgcctt acgatttttg agagccagcg caacatgttc
     5581 agaaagttcg cgtgttccaa aggtcgcata aggcgcgcaa ggaatagaat taccgccagc
     5641 cgccgcaatc atgtagtgaa tagcggggat cgatcggtta agaatggaaa ctgccgtgca
     5701 atgaacggca tgattgtgaa caaccgcgtt ggcatccggt ctgctttgat aggctgccat
     5761 atggaaacgc cattcgcttg aggggagctt tccttcctca tgtttaccgt tgccatcaat
     5821 aaagacaata tgcgactccg tcagtttttc atatggaatg cctgtaggcg taatcagcat
     5881 cccatcctga taacgtacac tgacgttccc cgctgtcccc tggttcagtc ccaggcgggt
     5941 catttccagg caagtgtcaa taatctgacg agcaagttta tttcgttcca ttagctacct
     6001 ctctctgatt caaaacaggg caataatgtt gttcctttca cactattgaa ttagccgttt
     6061 aattacccac catcttcttc ctgattaaca agaaagaaat tcacaagctt atattttgtg
     6121 acctagctca actaatcaca gtaaataact gcaagttctc tttttataac cccgttaaaa
     6181 atgaccgctc ttaaaaatat ttatcaaaac ggtcattttt ccatttcccc tcgccagaaa
     6241 tgcccgtttt cggcacaaac aattaatacg gtcatctggt ttgtgttttt tatgatttat
     6301 tttctgaaac gggcatgaaa tttcgattat taaagtgatg gtagtcacat aaagtcacct
     6361 tccagctaat aagtgtgacc gccgtcatat tacagagcgt tttttatttg aaaatgaatc
     6421 catgagttca tttcagacag gcaaatattc accgatatga agcccgaact cgctggtttt
     6481 gcacttttga aaacataacc gattacgtgc ttaagcttct gaacctaaga ggatgctatg
     6541 ggaaacacat caatacaaac gcagagttac cgtgcggtag ataaagatgc agggcaaagc
     6601 agaagttaca ttattccatt cgcgctgctg tgctcactgt tttttctttg ggcggtagcc
     6661 aataacctta acgacatttt attacctcaa ttccagcagg cttttacgct gacaaatttc
     6721 caggctggcc tgatccaatc ggccttttac tttggttatt tcattatccc aatccctgct
     6781 gggatattga tgaaaaaact cagttataaa gcagggatta ttaccggatt atttttatat
     6841 gccttgggcg ctgcattatt ctggcccgct gcagaaataa tgaactacac cttattttta
     6901 gttggcctat ttattattgc agccggatta ggttgtctgg aaactgccgc aaaccctttt
     6961 gttacggtat tagggccgga aagcagtagt cacttccgct taaatcttgc gcaaacattt
     7021 aactcgtttg gcgcaattat cgcggttgtc tttgggcaaa gtcttatttt gtctaacgtg
     7081 ccacatcaat cgcaagacgt tctcgataaa atgtctccag agcaattgag tgcgtataaa
     7141 cacagcctgg tattatcggt acagacacct tatatgatca tcgtggctat cgtgttactg
     7201 gtcgccctgc tgatcatgct gacgaaattc ccggcattgc agagtgataa tcacagtgac
     7261 gccaaacaag gatcgttctc cgcatcgctt tctcgtctgg cgcgtattcg tcactggcgc
     7321 tgggcggtat tagcgcaatt ctgctatgtc ggcgcacaaa cggcctgctg gagctatttg
     7381 attcgctacg ctgtagaaga aattctaggt atgactgcag gctttgccgc taactattta
     7441 accggaacca tggtgtgctt ctttattggt cgtttcaccg gtacctggct catcagtcgc
     7501 ttcgcaccac acaaagtcct ggccgcctac gcattaatcg ctatggcact gtgcctgatc
     7561 tcagccttcg ctggcggtca tgtgggctta atagccctga ctttatgcag cgcctttatg
     7621 tcgattcagt acccaacaat cttctcgctg ggcattaaga atctcggcca ggacaccaaa
     7681 tacggttcgt ccttcatcgt tatgaccatt attggcggcg gtattgtcac tccggtcatg
     7741 ggttttgtca gtgacgcggc gggcaacatc cccactgctg aactgatccc cgcactctgc
     7801 ttcgcggtca tctttatctt tgcccgtttc cgttctcaaa cggcaactaa ctgaacatat
     7861 tttccgaata aagtgaggaa tctgtaatga aaaaaatcag cttaccgaaa attggtatcc
     7921 gcccggttat tgacggtcgt cgcatgggtg ttcgtgagtc gcttgaagaa caaacaatga
     7981 atatggcgaa agctacggcc gcactgctga ccgagaaact gcgccatgcc tgcggagctg
     8041 ccgtcgagtg tgtcatttcc gatacctgta tcgcgggtat ggctgaagcc tctgcttgcg
     8101 aagaaaaatt cagcagccag aatgtaggcc tcaccattac ggtaacgcct tgctggtgct
     8161 atggcagtga aaccatcgac atggatccaa cccgcccgaa agccatttgg ggctttaacg
     8221 gcactgaacg ccccggcgct gtttacctgg ctgcggctct ggcagctcac agccagaaag
     8281 gcatcccggc attctccatt tacggtcatg acgttcagga tgccgatgac acatcgattc
     8341 ctgccgatgt tgaagaaaaa ctgctgcgct ttgcccgcgc cggtttggcc gtcgccagca
     8401 tgaaaggtaa aagctatctg tcgctgggcg gcgtttcgat gggtatcgcc ggttctattg
     8461 ttgatcacaa cttctttgaa tcctggctgg gaatgaaagt ccaggcggtg gatatgaccg
     8521 aactgcgtcg ccgtatcgat cagaagattt acgacgaagc cgaactggaa atggcactgg
     8581 cctgggctga taaaaacttc cgctatggcg aagatgaaaa taacaaacag tatcaacgca
     8641 atgccgagca aagccgcgca gttctgcgcg aaagtttact gatggcgatg tgtatccgcg
     8701 acatgatgca aggcaacagc aaactggccg atattggtcg cgtggaagaa tcacttggct
     8761 acaacgccat cgctgcgggc ttccaggggc aacgtcactg gaccgatcaa tatcccaatg
     8821 gtgacaccgc cgaagcgatc ctcaacagtt catttgactg gaatggcgtg cgcgaaccct
     8881 ttgtcgtggc gaccgaaaac gacagtctta acggcgtggc aatgctaatg ggtcaccagc
     8941 tcaccggcac cgctcaggta tttgccgatg tgcgtaccta ctggtcacca gaagcaattg
     9001 agcgtgtaac ggggcataaa ctggacggac tggcagaaca cggcatcatc catttgatca
     9061 actcaggttc tgctgcgctg gacggttcct gtaaacaacg cgacagcgaa ggtaacccga
     9121 cgatgaagcc gcactgggaa atctctcagc aagaggctga cgcttgcctc gccgctaccg
     9181 aatggtgccc ggcgatccac gaatacttcc gtggcggcgg ttactcttcc cgcttcctta
     9241 ccgaaggcgg cgtcccgttc accatgactc gtgtcaacat catcaaaggc ctgggaccgg
     9301 tactgcaaat cgcggaaggc tggagcgtgg aattgccgaa ggatgtgcat gacatcctca
     9361 acaaacgcac caactcaacc tggccaacca cctggtttgc accgcgcctc actggtaaag
     9421 ggccgtttac ggatgtgtat tcggtaatgg cgaactgggg tgccaaccac ggagttctga
     9481 ccatcggcca cgttggcgca gactttatca cccttgcctc catgctgcgt atcccggtat
     9541 gtatgcacaa cgttgaagag accaaagtgt atcgtccttc tgcctgggct gcgcacggca
     9601 tggatattga aggccaggat taccgcgctt gccagaacta cggtccgttg tacaagcgtt
     9661 aatacattac accctgatgt gatgcccggt cgctccggct accgggcctg aacaagcaag
     9721 agtggttagc cggataaaca atgttatccg gctatattgc aggagcgatt atgaaacaag
     9781 aagttatcct ggtactcgac tgtggcgcga ccaatgtcag ggccatcgcg gttaatcggc
     9841 agggaaaaat tgttgcccgc gcctcaacgc ctaatgccag cgatatcgcg atggaaaaca
     9901 acacctggca ccagtggtct ttagactgcc attttgcaac gctttgctga ttgctgtcgg
     9961 caaatcaata gtgaactgac tgaatgccac atccgcggta tcgccgtcac cacctttagt
    10021 gtggatggcg ctctggtaga taagcaaggc aatctgctct atccgatcat tagctggaaa
    10081 tgtccgcgaa cagcagcggt tatggacaat attgaacggt taatctccgc acagcggttg
    10141 caggctattt ctggcgtcgg agcctttagt ttcaatacgt tatataagtt ggtgtggttg
    10201 aaagaaaatc atccacaact gctggaacgc gcgcacgcct ggctcttcat ttcgtcgctg
    10261 attaaccacc gtttaaccag cgaattcact actgatatca cgatggccgg aaccagccag
    10321 atgctggata tccagcaacg cgatttcagt ccgcaaattt tacaagccac cggtattcca
    10381 cgccgactct tccctcgtct ggtggaagcg ggtgaacaga ttggtacgct acagaacaac
    10441 gccgcagcaa tgctcggctt acccgttggc ataccggtga tttccgcagg tcacgatacc
    10501 cagttcgccc tttttggcgc tggtgccgaa caaaatgaac ccgtgctctc ctccggtaca
    10561 tgggaaattt taatggttcg cagcgcctag gttgatactt cgctgttaag tcagtacgcc
    10621 ggttccacct gcgaactaga tagccaggca tggttgtata acccaggtat gcaatggctg
    10681 gcatccggcg tgctggaatg ggtgagaaaa ctgttctgga cggctgaaac accctggcaa
    10741 atgttgattg aagaagctcg tctgatcgca cctggcgcgg atggtgtgaa aatgcagtgt
    10801 gatttattgt cgtgtcagaa cgctggctgg caaggagtga cgcttaatac cacgcggggg
    10861 catttctatc gcgcggcgct ggaagggtta actgcgcaat tacagcgcaa tctacagatg
    10921 ctggaaaaaa tcgggcactt taaagcctat gaattattgt tagtcggtgg aggaagtcgc
    10981 aacacattgt ggaatcagat taaagccaat atgcttgata ttccggtaaa agttctcgac
    11041 gatgccgaaa cgaccgtcgc aggagctgcg ctgttcggtt ggtatggcgt aggggaattt
    11101 aacagcccgg aagaagcccg tgcacagatt cattatcagt accgttattt ctacccgcaa
    11161 actgaacctg aatttataga ggaagtgtga aatgctgaaa acaatttcgc cgttaatttc
    11221 tcccgaacta ttgaaagtgc tggcagagat gggacatgga gatgaaatta ttttttccga
    11281 tgctcacttt cccgcccatt cgatggaacc gcaggtgatc cgcgctgatg gcctgttggt
    11341 gagcgacttg ctccaggcga ttatcccgtt atttgagctg gacagttatg ccccgccgct
    11401 ggtgatgatg gcggcggtag agggtgacac tctcgatcct gaagtagaac gacgttaccg
    11461 taatgcgctt tcactacaag ccccgtgtcc tgacatcatc cgcatcaatc gttttgcgtt
    11521 ttatgaacgg gcgcaaaaag cctttgcgat cgttatcaca ggcgaacgag cgaagtacgg
    11581 gaatattctt ttaaaaaaag gggtaacacc gtaatctcat accggtacgc ccgcatgacg
    11641 cgggcggtta tcgaatgatg gggtgaaaaa tatgaaagcg gcacgccagc aagcgatagt
    11701 cgacctgctg ctgaaccata ccagcctgac cacggaagct ctctctgaac agctaaaggt
    11761 cagtaaagaa accattcgtc gcgatctcaa tgaattacag acgcagggta aaattctgcg
    11821 caatcatgga cgcgctaaat atatccaccg tcaaaatcaa gacagtggcg atccctttca
    11881 catcaggctg aaaagccatt atgcgcataa agcagatatc gcgcgcgagg cgctcgcgtg
    11941 gattgaagaa gggatggtga tagccttaga cgccagttca acttgctggt atctggcacg
    12001 ccagttgcct gacatcaaca ttcaggtctt caccaatagc catccgattt gccatgaact
    12061 cggtaaacgc gaacgcattc aactgatcag ttccggcggc acacttgagc gcaaatatgg
    12121 ctgttacgtc aatccctcgc tgatttccca acttaaatcg ctggaaatcg atctgtttat
    12181 tttttcttgt gaagggatcg atagcagcgg cgcactgtgg gactccaatg cgatcaacgc
    12241 tgattacaaa tcgatgctat taaaacgtgc cgcgcaatcg ttgttattga ttgataaaag
    12301 taaatttaat cgttcagggg aagcccgcat cgggcatctg gatgaggtaa cgcacattat
    12361 ttctgatgag cgccaggttg caacttcttt ggtaacagcc tgacggaaag ggtagcaggc
    12421 cggagacgac gccccggcct tgcctgttat cgctcgtcgc gacgaccacc caccgcagcc
    12481 cagatgcggc ggacgtgcac cgtcacttct tcgcgatcgt gatacaactg ccgcgcctga
    12541 atctgagcat ttatgccatg ttcatcaagc tgtgcctgaa tatacgccag attgtgtgat
    12601 acttcttcgt agcgtttttt catcggcagt ttgagattga aaatggtttc acggcaccag
    12661 ccattaacca gccactgcgc catcaatgcc gcaactttag ccggtttttc aaccatatcg
    12721 cataccatcc aggagatatt gctgcgtgtc ggacggaatt tgaaaccgtc ttcccgcagc
    12781 cacgtcacct gcccggtatc catcagactt tgcgccatcg ggccgttgtc gacggaataa
    12841 acccacatgt tgcgcttcac cagttggtag gtccagccgc cagggcaagc gcctaaatcc
    12901 accgcccaca tcccgttcgc caggcgttca tcccactcat ccgcaggaat aaacacatga
    12961 aatgcctctt ccagtttgag cgtggaacga ctcggcgcct ctgccggaaa tttcaggcgc
    13021 ggaatgccca tatagaacgg cgaattattg ttgctgtatg agtaaccggt atagcagcat
    13081 cctggtgcaa taaagaatac atgcacaacc ggacgcttcg gcgtttcata gttcgccagc
    13141 accccagcat cgcgcaaggc agcgcgtagc ggaacagtga acttacggca gaatttcagc
    13201 aactctttgc tttcgttggt atcggcaact tcaacacgca gttcaccgcc cttctctact
    13261 acaccctgta acatgccgac aatgggggta atacgatctt ctggcggcaa atgctgcagg
    13321 agttccccca ccacaaacca ctggcgggca aaaattaatg aactgaacgg cagctcacgg
    13381 attaacttat cgccatcatc aggttgataa cattcataaa tgacataacc cgcattctct
    13441 ttcacgcggg caaaaccgaa aatttcccgc tggccggctt tatcggtaat ttctgcggcg
    13501 cactcttttt caaagcccgg acggcacagc aatacaacct tattcatgac ttacgccctt
    13561 gcgctttaaa cggatagcac caactaacat taacgcccag cccgcaagga agctcacgcc
    13621 gccaaccggt gtgacaaacg cccacaaacg cagatgggac agcgccaggc aataaaggct
    13681 gccgctgaac agcaccgtgc ctaacgcgag gaaaacgcta ctccagtaaa accagatact
    13741 gatgcgacgc tgcattgcca ccgccagacc taagatcgcc agcgtatgaa acgcctggta
    13801 ttcgagaccg gtttggatcc agcccatctc aacggccccc atggttttac ttaacacatg
    13861 cgcaccaaaa gcgcccagtg ccacaaaaat gaagccgcta atggcggcga aaatcagcat
    13921 aaaacggctg gtcatggtcg taccctacgt aaattattgt tcataacgaa agcggaattt
    13981 ttcttgttca gcagcggctt tcgccaggat ccattggcga aaggcggcta ttttacccag
    14041 ttctgcctgg ctgtcatgac aaaccagata aaaagcattt ttactgacca gaacatcatt
    14101 aaacgggcaa acaagacgtc cggcctcgat ttcagattgc gccatcacgt tatttgccag
    14161 cgccactccc tgcccgtgaa tagccgcttg cagcaccatg gcgctatgac taaaaattgg
    14221 cccttgctga acgttgatat gattaaaccc caactgtcgg gtatatgtct gccagtcacg
    14281 gcgtgacgca tcatgtaata acgtatgttt agccagatct tccggggtct tcaagggttt
    14341 ttcgcctgtc agcagtagcg gcgaacacac cggcaataaa tattcggcgt acagtttttc
    14401 cacccgtagc cccggccagt tgccccgacc ataaaatatc gccacatcaa catcatccgc
    14461 cagcttatct tcctgacgat caaccgcctg gattcgaacg tcaattcccg gataagctga
    14521 attaaagctg gaaagtcgcg gaaccaacca atgaatggcg aaactgggga gtaaactgac
    14581 cgtcaacgcc cccttggcgc tacgggcctg gagtttacgc gtcgcttcgg ttaattgcga
    14641 aaatatctct ttgatatcga ggaaatagct ttgcccttcc tcggtcagca ggagtgaacg
    14701 attacggcgg cggaacagtt ttagccccaa aaaatcctca agagacttga tttgatgact
    14761 tactgcggcc tgggtcacaa aaagctcttc tgctgcgcga gtaaaactta aatggcgtgc
    14821 tgcggcatca aaaactcgta aggcatttag cggtggtaat cgtttagaca tggcttttaa
    14881 actttgatgt caaatgaatt taacaattag atcacactgt gtaacctatt agttttttta
    14941 atctgagcca ttataaattg tccgttgagc ttctaccagc aaatacctat agtggcggca
    15001 cttcctgagc cggaacgaaa agttttatcg gaatgcgtat tctggtgaac ttttggctta
    15061 cggttgtgat gttgtgttgt tgtgtttgca attggtctgc gattcagacc atggtagcaa
    15121 agctaccttt tttcacttcc tgtacattta ccctgtctgt ccatagtgat taatgtagca
    15181 ccgcctaatt gcggtgcttt tttttacctt gcgatcgcga attactgttc cagttcgacc
    15241 atctctttca cgtcagtacg gttgatctgc tgtttgttgc cattagcgtc tttatacgaa
    15301 atcataccga tatcgttatc agtctgtggt ttgccgtcag agacgatggt acgtccgtca
    15361 ttggtgtgca tcacatagtt cgaaccggaa caggcgctca gggcaaaagt cagcatacag
    15421 gcagaaataa ttgcggcagt ctttttcatc atcttctcct taaaacgatt aataaatctt
    15481 aatagtattg attaacaggc taaaattaac gcctaacact attcagcata tgttacttac
    15541 gcggcttcgc caggatatcc agataattct gatggttagc actctccttg tatcaaagtg
    15601 aattttgcgt cacgatcggt gcatcaagcc gaggagtacc atgaacgttt ttaatcccgc
    15661 gcagtttcgc gcccagtttc ccgcactaca ggatgcgggc gtctatctcg acagcgccgc
    15721 gaccgcgctt aaacctgaag ccgtggttga agccactcga cagttttaca gcctgagcgc
    15781 cggaaacgtc catcgcagcc agtttgccga agcccaacgc ctgaccgcgc gttatgaagc
    15841 tgcacgagag aaagtggcgc aattactgaa tgcaccggat gataaaacta tcgtctggac
    15901 gcgcggcacc actgaatcca tcaacatggt ggcacaatgc tatgcgcgtc cgcgtctgca
    15961 accgggcgat gagattattg tcagcgtggc agaacaccac gctaacctcg tcccctggct
    16021 gatggtcgcc caacaaacta gagccaaagt ggtgaaattg ccgcttaatg cgcagcgact
    16081 gccggatgtc gatttgttgc cagagctgat tactccccgt agtcggattc tggcgttggg
    16141 tcagatgtcg aacgtgactg gcggttgccc ggatctggcg cgagcgatta cctttgctca
    16201 ttcagccggg atggtggtga tggttgatgg tgctcagggg acggtgcatt tccccgcgga
    16261 tgttcagcaa ctggatatcg atttctatgc tttctctggt cacaaactgt atggcccgac
    16321 gggtatcggc gtgctgtatg gcaaatcaga actgctggaa gcgatgtcgc cctggctggg
    16381 tggcggcaaa atgattcacg aagtgagttt tgacggtttc accactcaat ctgcgccgtg
    16441 gaaactggaa gcaggaacgc caaatgtcgc tggcgtcata ggattaagcg cggcgctgga
    16501 atggctggca gattacgata tcaaccaggc cgaaagctgg agccgtagct tagcaacgct
    16561 tgcagaagaa gcgctggcga aacgtccagg ctttcgttca ttccgctgcc aggattccag
    16621 cctgctggcc tttgattttg ctggcgttca tcacagcgat atggtgacgc tactggcgga
    16681 gtacggtatt gccttgcggg ccgggcaaca ttgcgctcag ccgctactgg cagaattagg
    16741 cgtaaccggc acactgcgcg cctcatttgc gccatataat acaaagagtg atgtggatgc
    16801 gctggtgaat gccgttgacc gcgcgctgga attattggtg gattaatgac aaacccgcaa
    16861 ttcgccggac atccgttcgg cacaaccgta accgcagaaa cgttacgcaa taccttcgca
    16921 ccgttgtcgc aatgggaaga taaatatcgc cagttgatca tgctggggaa acagcttccg
    16981 gctttgccag acgagttaaa agcgcaggct aaagagattg ccggatgcga aaaccgcgtc
    17041 tggctgggat ataccgtggc tgaaaacggc aaaatgcact tctttggcaa cagcgaaggg
    17101 cgcattgtgc gcggcctgtt ggcggtgttg ttgactgccg ttgaggggaa aaccgccgcc
    17161 gaattacagg cacagtcgcc gctggcattg tttgatgagc tgggattacg tgcgcagctt
    17221 agcgcctcac gcagccaggg gttaaatgcg ttaagcgagg cgattatcgc tgcgacgaag
    17281 caggtttaag cacagaaccg ggtcggataa gacgttcgcg tctcatccga cctgattgtt
    17341 taaccctgac gcgccgcttt cgccatcatc ttcttcagcg catgagaaac cgcaacaaac
    17401 ccaaaggtgg cggtcaccat cgttgccgcg ccaaatccag atgcacaatc catccgcttc
    17461 ggcccttctg ccgtggcttt catcgcacac accgtaccgt ctgactgcgg gtacaccagc
    17521 gcttcagtag aaaacacgca atccacgccg agcttacctt tactgttttt cactacgcca
    17581 aaatcgcttt tcaggcgctc gcgcaacttc gccgccagcg gatcctgaat cgttttcgcc
    17641 agatcggtaa cctgaatctg cgtcgggtca atctgcccac ccgcgccacc ggtcgtgacc
    17701 agcgggattt tattgcgccg acaataagca atcagcgccg ctttgggccg tacactatca
    17761 atggcatcaa tcacgtacga ataaccggca ctcatatatt gcgctacgtt atccggcgtc
    17821 acgaaatcat ccaccaccgt tacacggcac tccgggttaa tctggcgaat gcgctccgcc
    17881 atgacttccg cttttgccag cccgacgtta tcgcgcagag cgtgaatttg ccgattggta
    17941 ttggtgacgc acacatcatc catatcgata agcgtgattg cgccaatccc cgtgcgcgcc
    18001 agcgcttccg ccgcccagga accgacacca ccgataccaa ccacacaaat atgcgcgtca
    18061 gcaaacagtt gcaacgcttt ttcaccatac agacgcgctg tgccaccaaa acgctgacgc
    18121 catgcatcac taattaccac agacatagca cctcagaatt aaaaagggcg aggttatccc
    18181 tcaccctaaa cgcataaatg cctgatgcgc tacgcttatc aggcctacat aactcctgca
    18241 atattttgga tttgcatgat ttgtaggccg gataaggcgt tcacgccgca tccggtatga
    18301 acaacgcgca cttagtcaat aatcaggatg tgaggttacc cctcccctgt catatccgta
    18361 aaaacggcat acagaatatc acatcagccg ctaaagacgt tacctgcgcc cggagccgtt
    18421 ttcagcaccc aaacccgtcc atagtggttg taccaacctg cgcggtgtcc ggcttccggc
    18481 ccgatccctt gatagatatc gaagtgttgg cctttgattg cgcccccgac atccaacgcc
    18541 accatcagac gcaattcgta ctgaccgtta aatttgccgt tattatccag cagcggcact
    18601 tctgccagca aggtagtacc tggcggaata attgaacgat cggaggcaac agaagcacgg
    18661 ccaaccagcg gcaccgcact tgcccctttc accggtgcaa aagattgcgg tttaaagaag
    18721 acgaaagacg ggttctgttc cagcagctcg cgaacctcgg cttcactgtg tgtttcgccc
    18781 caatgacgaa tcgcctgcat cgacatatct tcttttttca cttcgccacg gtcgatcagc
    18841 accttaccaa tgctgcgata ggcatgaccg tttttccctg catagctgaa aaagttaagc
    18901 ggactgccat caccaaagtc gatataccca ctaccctgca catccataat gaagttatcc
    18961 atcagagagt tgctgtaagc gaggatgtat ttatcgccca atgccccggc gtagatctcc
    19021 gcacgagacg gcagacgacc acgttttggc ggcatacggt aaataggata ctggaactcg
    19081 ccctggcggg tatggcgcgc ctgaattacc ggcgtgtaat agcctgtaaa ctgcacgtta
    19141 ccatagttgt cggcaccttc catctgccag gcatcaatgc cgaactggcg catattgcgg
    19201 gtatcaccgc ctgcgcgcag ccactcttgc accgcgttat aaacattact ctggttgcca
    19261 tacagacgcg gtgacgaatt acggatatgg ttaatttgct cgacaaaatc accggcgtta
    19321 atcggcgcgc caacggcatc tggctggttc accagagaga aaggctgggt aaatttcccg
    19381 tctttatatt gctgtccgcg atcggttggt ttggaagagc aggcggcaag cattgccaca
    19441 accgtgccca taagaaggta ctttacccaa cgtcctttca ttgttctctt cttcaggtta
    19501 aaaaataagg cgcaacgaag ataacaaacc gccgcgttta atgaaatgag cagcctctcc
    19561 ctgacgcaaa ttttgcacaa aaaataggct ttagtggttt gtttttgttc aaaatcatgc
    19621 taaatccgtg atcggggtaa aaaaaaggtt gcatgaaaac gcgagcggag tatagtgcgc
    19681 atccacggac gcggggtgga gcagcctggt agctcgtcgg gctcataacc cgaaggtcgt
    19741 cggttcaaat ccggcccccg caaccaatca aatttgatga agtaaaagca gtacggtgac
    19801 gcggggtgga gcagcctggt agctcgtcgg gctcataacc cgaaggtcgt cggttcaaat
    19861 ccggcccccg caaccaatta ttgaacaccc taacgggtgt ttttttgttt ctgatctccc
    19921 ataaaaaaag cgccattcag cgccttttta tcatcccctt ctcgccagcg ttgccccatc
    19981 ggcaaaatac gctttaatcc ccgcaagaat agactccgca acttcctgct ggaaagtcgc
    20041 cgttttcagt ttacgctctt cctcaacgtt actgataaac gccgtttcga ccagaatgga
    20101 gggaatatct ggtgccttta gtacggcaaa cccggcctgt tcaacttgat ttttatgcag
    20161 cttgttgatt tttccgagct tattcagtac cgctttacca aacttaaggc tgtcagcaat
    20221 agtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac cgcttttgct
    20281 cacgccacca atcaagtccg aggcgttctg ggtttgtgcc agatattttg ccgcagtact
    20341 ggttgcgcct ttggttgaga gcgcaaacac cgaggaaccg ctcggctgac gactggtaaa
    20401 ggcgtcggca tggatagaga caaacaggtc agcacgctgt ttctgggctt ttgctacgcg
    20461 cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca tattgccctc
    20521 ttcctcgatc agagagcgca aacggcgagc tatttgcaat accacatctt tttcgcgcgt
    20581 tttgtatttc cccaccgcac cggagtcttc gccaccgtgg ccagggtcaa gcataatgac
    20641 aatcggacga tcgcgccctg ctttacccgg ttgtggacca ctttgcgctg gcggcacctg
    20701 cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt cctgcatatc
    20761 ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg cgaccggcgc
    20821 aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatgc gtacggtttg
    20881 cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac gaatttgtgc
    20941 cgccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca ccacgcgttc
    21001 agggttactc aacgcgaact gcttatattt cagctgacga tttgattcta ccgtcacgcg
    21061 ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctaaccg cagccaggct
    21121 gacctgactt acgctcaata gccacatagc gcccgcgcct tgcagtaaac gacgacggct
    21181 gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa gctgtgtaac
    21241 aagtaaacga ctaatttgac cggaaacttt agcgaaagac gcataatctg tcatctaata
    21301 aacggtaaac attcttttta tattcacggc attactgata aaaaagtcgc tctcgcataa
    21361 aatttacact tgcaccctgc gaaaaaacag aataaaaata caataatttc aaataatcat
    21421 gcaaaggggt gtgccgtggt aaaggaacgt aaaaccgagc tggtcgaggg attccgccat
    21481 tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct cggcggtgaa
    21541 gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttgtt gcacagcctc
    21601 ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa tctggctgcg
    21661 catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa aacactggaa
    21721 ctggtgaagc aggctgcggg aacattgcaa ctggatatta ctgctcgcct gtcaatgagt
    21781 ctcaataaca cgccgctgca gggcgcgcat atcaacgtcg ttagtggcaa ttttattatt
    21841 gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg tatccggcgg
    21901 attgatgaag acgcgctcca tcgtcaactg gaaagcggtg caatagtgct aatggggccg
    21961 gtcgctgttt cagtcactgg cgagagcttt aatctgacct cggaagagat tgccactcaa
    22021 ctagccatca aactgaaagc tgaaaagatg attggttttt gctcttccca gggcgttact
    22081 aatgacgacg gtgatattgt ctccgaactt ttccctaacg aagcgcaagc acgggtagaa
    22141 gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttgcg tggcgcagtg
    22201 aaagcctgcc gcagcggcgt gcgccgctgt catttaatca gttatcagga agatggcgcg
    22261 ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat ggaaagcgcc
    22321 gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt gattcgcccg
    22381 ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat ggaaatcgac
    22441 aaattcacca ttattcagcg cgataacacg actattgcct gcgccgcgct ctatccgttc
    22501 ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta ccgcagttca
    22561 tcacggggcg aggttctgct ggaacgcatt gccgctcagg cgaagcagag cggcttaagc
    22621 aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg atttaccccg
    22681 gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca gcgtaaatcc
    22741 aaagtgttga tggcggattt agggtaagcg atgaaaatca ccggatgcgg caggcgtaac
    22801 actcgtgcgt cgcatccggc aattacgttt attcccgcga actaaacaac gccgccagac
    22861 cactgcgccg ctcagtacga gtggcgattg ccgcacttaa tatacgctca tcggcataca
    22921 gcgacagacg acgacgcgcg cgggtcaccg cggtgtatac cagctctcgc gttactaccg
    22981 gcgtgcgttg gctcggtaaa atcaacgccg catggtcgaa ctccgatccc tgcgatttat
    23041 gtaccgtcat cgcccacgta gtttcgtgct ctggcaagcg actcggttgc acagacttaa
    23101 tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc agcgcaatac
    23161 cgatatcgcc attaaacaac ccaagcgcgc tgtcattacg ggcaatcatc accggtcggc
    23221 cttcgtacca acgagagtgc ggatgacgat gaattttgcg cttctgttgc ataaactgtt
    23281 caattcgctc attcagtcca gccacgccaa acggcccttc ccgcagggcg cacaaaagct
    23341 ggtactcatt gaacgcctga atgattaaat ccggctcggc acgcgcttgc agcagatcca
    23401 gataacgtcc gtaacccgca agagcttcct caagcatcgc aatataatct tcgccgctct
    23461 gtaaaagtcg tttttcgata tcagtaaaat cctgctgaaa aacggttttc actgccgttt
    23521 tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcgctg ccgaaacgat
    23581 agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg ccggttcctg
    23641 ccggaacgtg agtccccgtc aggcggctta gctgcctggc acgctcggcg gtaaagcccg
    23701 cgttggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag gccagttgat
    23761 cacgatcgcc gagaaagatc actcgcgcat gatctggcaa ggcgtcgatc agtctcgaca
    23821 tcataggcag atcgatcatt gacgcttcat ctaccaccag cacatcaaga tgcagcgggt
    23881 taccggcatg atgacgtaaa cgctggctac ccggctgcgc gcccagcaat cggtgcaaag
    23941 tgctggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaac tgtcgcaaag
    24001 ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca gccagacgga
    24061 tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagcaacttc gccacggtgg
    24121 tcgttttacc ggtgcctggg ccgccggaaa tcaccgagat ccgccgcgtc agcgccactg
    24181 ccgctgcaac tttttgccag ttaatttcat cgcttactgg aaaaagtttg tccagggttt
    24241 gcgccagtag agcttcatca acctcaatgg tatgattcac ctcattgaag aagcgcgcta
    24301 ccgtccgctc gttacaccac atgcgattca aataaagacg atcgccacag aggatcatcg
    24361 gcgtgggttc atctcccctg ctgaccgctt gagaagccag caagcattct tcccaatttt
    24421 gtagctcacc gatttcactg acacaggtcg ccaacagcgg attcgacgcc tcgttatttt
    24481 ccagtcgtga aagcggcaaa cagacgtgtc cctctccggc atcatgactt aacagtgccg
    24541 ccgcgagggt gacggcagga tgttcatctc ccgccacggt cagggcaaat tgcacatcca
    24601 gcgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat ttcattacgc
    24661 ctcctccagg gtcataccgg caaacatctc atccatcagg gcaatcaacc cggcgttggg
    24721 tcgggttgtg taaatccctt gttgcggatg ttctttatca acgccacgca ggaacagata
    24781 gataacgccg ccaaagtgaa gatcatagtc gtaatcagca atgcgatggc gcagataacg
    24841 atgcagcgcc agggtataaa gctgatattg cagatcatag cggtgtgcct gcatcgctgc
    24901 tgccatagcc tgttgggtgt aagccgaact gtcttcaccc agccagttgg atttatagtc
    24961 gagcaggtaa taacgccctt cgtggcggaa taccaggtcg ataaagcctt ttaacatgcc
    25021 gcgtacctgc atgaactcca gcggcgggca gccagcggat agcgggtcaa actggcgaat
    25081 tagtgcatca agctggctgg cgatgagcgg ttcgctaatc ggcaggtaaa actccatctc
    25141 cacctgttta tcgcggtcgg aaagctgact caggctaaca cccgtttcat tgagaggtgc
    25201 ctggaggaca gccgtgatcc actcggtcaa caccggttcc cactgcgatt caaagccgcc
    25261 gagttccagt ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat cgaggtcttc
    25321 aaacaaactg tgcaagaatg tccccggtga cgcaccgcgc gggaactgat gcggtgttaa
    25381 cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc gtggcatcag
    25441 atcctgagcg ataccgtgac cacgctgctg caagccggag tagctggtga cacgccagtt
    25501 atcgccgggc aatcgttgta gtgtcctcgc attcagttct gcagttaaag catcattaac
    25561 ctgccagggc tggttatcac cagtttgtgc cgtttgccag gcaatatcat catcgcataa
    25621 cgcttcaata caggtgcgaa gccctgccgc atcttgcggt tccccttttt gcagcaaacg
    25681 cccgagcgca ctttggtgga cgtcggtgtc acctttttta tcgccacgac ggcgcaccag
    25741 cggtgcaacg ccgagactgc aatgccaaac cgaacgcgtc agcgccacgt aaagcaaacg
    25801 cagatcttcc gccagacgtt cgacctccgc gaggtcgacg ctttctggcg cagcattaag
    25861 atccaaaact gcctcaaacg agtggcgatc gtgataaaac gcctgatcct ggacgcggaa
    25921 attggtaata aatggcaacc agaccagcgg atattccagc cctttcgatt tatggatcgt
    25981 gacaatctgc accagatgtt tatcactttc gaggcgcatt tgttggctgg aggcattact
    26041 gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac tttccagctg
    26101 cgttccggct tcttgaagca gttcgctgat atgcaagata tcggtaagac gccgctcacc
    26161 gcctgccgtt gccagcaagt tttctgcaat gttacgcgcc gacatcagcg cccgcagcat
    26221 cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt cgactaccgc
    26281 atcccacgca tgttcgtcat tattcagcgt ttcaatatcc agcgcattca gccccatcat
    26341 tgacgttgcc agcgcactgc gcagggtgtt ctcacgttcg ggcgtcatca ccgcctgcaa
    26401 caaccaaagc atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt tcgaaaggta
    26461 aacggaaggg atttccagca acgttaaggc atcgcgcacc tgggcggcct cctggcggct
    26521 gcgcaccagc acgctgatgt ccgaagcacg caccggacgc gcgtcgtcgc cgttcatcag
    26581 caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag cacatacctg
    26641 cgccatggta ctttgatagt cgccaacgcc gcagctttcg ccttccatca gccacatttt
    26701 catcgcaggc tgcgtttcac ctttaaatac aaaacgtaac gcctgatttt tcccggctga
    26761 tttcactgga ataaacggta tttcgcgaaa catgaacgtg tcatcagtct ggctgaaaag
    26821 cttgttcaca ctgttcacca ttccaggtgc ggagcgccag ttggtgtcta aagtgtagtg
    26881 ggcatgaact tcactacgcg ccttcatata agtgaagata tccgcacccc ggaatgcata
    26941 tatggcctgc ttcgggtcgc caatcagcaa caatgcggtt tccggctgat ggtgccagat
    27001 acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcga tcattgccac
    27061 cgggaatcgc gtacggatcg ccgctgccaa cacctcgccg ctttcgctac gcagcgcgga
    27121 atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt tttcacgcgc
    27181 tactgtttcg cggatctcag ccaatgcgcg ggtgatcaac agatcgcgga tcgacaatgg
    27241 ttctgcaagc agttgatcga tcgcctcaaa cagtggatgt cgcggggttt cccccccggc
    27301 cttcgtgcga tcttctaaga aacgctggga gaatttttcc agcgactccg gcaactgata
    27361 actgtttgtc tcttcttctg cccaggcgct gatcttttcg atccatttag cctgattgct
    27421 acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca gttcacccac
    27481 tgcgtcgcgc cactgctgtt ttaccgtatc aatacgcgcc acaatttgcg cgtggcggga
    27541 agccagcgtt tcatcatcgg gcggcggtgc tttgataacc ggcggttcgc cttgcagata
    27601 acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga cgacctgggc
    27661 tatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg cctggtagcg
    27721 tagcagagac tcatcttcaa tcagctgctg ctcaaacagc atgccggatt caaaggcatt
    27781 caggttgagc atgcgctggc aaaagccgtg aatagtaaag actgccgctt catccatctg
    27841 ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct cttccagcag
    27901 gcgttcgtac agtggattgt cggtggtttc acgcagacag gcgatgcgca actcgtggat
    27961 attgctacgg atacgaccgc gcaattctgc cgtggcagcc tcggtaaagg tgaccaccag
    28021 cagttcttca acgatcagcg ggcggggaaa ggcggcggaa ccgcctagtc caagtaacag
    28081 gcgcaaatag agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg cttcaatcag
    28141 gcgctcgccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat cactcattct
    28201 tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac actttccagc
    28261 cttcaggatg cacatattcg gctttcccgt tctggctgcc ggaaatctgc gacagaatag
    28321 ccatgccttg cggctcgacc accgcctgat ggaagaaatc agcaagtttt tgcggcgtca
    28381 gcagttttat ctgggccacg attttatcac gcgaatcgaa gcgcatattg ccgcgatcga
    28441 aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc agcatctggg
    28501 taattaccgc ctgctggatt tgcgcaaact catctggctt catcgctcgc aattttgcct
    28561 ctgcggttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt ttatcattgc
    28621 tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaac gcaaacacgg
    28681 catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt acgatctgcc
    28741 ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt acaaataccg
    28801 ctgccagtgc ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat tgttttttat
    28861 cgaccactac atctttgttt cgacaccact ctgaaccatc agcgcccaac tgtttttgca
    28921 catcgcgtgc cagcgttgtt gcctgggcct cggtcatgtt gccgataacc ataaactctg
    28981 gtcgagcccc tgattttaag gcgtcgcgat aggccagcac ctctttcaac gtaatggagg
    29041 gcaaaatttt acgccgttca tctcgcgaga agtacggcac ttgcgagagc atctgcgcgg
    29101 gcataatcgc ctgctcaaac gctttaccct tttctgcgga atccatcatc tggttatacc
    29161 aggacttcgc ctgctcaagc tgctcttccg tagcggtata gctaaagtac ccctcgagca
    29221 atgcctggaa cagctgcggc agacgctggg tgtaaccatt agcattaatc ataaggccgt
    29281 tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt aactgatcaa
    29341 gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg gcgctgtcca
    29401 tggctttcgg attacgcaaa atcaggctga catcagcttt gggctcgctg gcaaaataac
    29461 ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcagctct ggatggtcgt
    29521 atttcttctc tgacttaatc agcgagaaat catcaggaat ataagggtta agctctggca
    29581 aagagagcgc aatgtcggcg gcttttttct gccagtcggc gaaagtttgt gcgctgattt
    29641 tatcgacctg atacggcgca tcgacaaagt aagccgtttt gttgtgcggc tctttcgggc
    29701 tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttcc tttactgctt
    29761 tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaaca ggaacgcgaa
    29821 tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgatcgac ggataacgga
    29881 agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcaatg cctttttcac
    29941 gtaacagatt gagatagcta aaaattgccg ccacaacctg atcgcgatta gccaggcctt
    30001 tatcagttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg ataggatcgg
    30061 agttggcgct aatgccctca actaatccct gcttttgcag ccagtcagaa agtgtacctg
    30121 ggctgcgatt gccaatcaga taggtaatca attcatcggt tttactacgg aacttcgctg
    30181 agttgttatc gatgcgaaac tcaacgcgca acactttacg cggcagcgca gggacgtaat
    30241 gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc ggttttttgc
    30301 tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac tccggcagcg
    30361 gtttattact gtaaataacc gccttcatca aattggcgga atagtacttc tcgtggaaat
    30421 ctttcagcgc ctgctgcacc ggattaccag gtttgtcgct taaagtttcg aggttaccac
    30481 cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc tgtgccatgc
    30541 gcatcccgtc acgcgtacgc gccatggtta attcagcgtt cactgcatta cgctcacgtt
    30601 cggcgtattt cttgtcgagc aagggttctg caatagcatc ggccaggcgg tctaccgcac
    30661 cgggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc gcagtgctgg
    30721 cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc tgcgggtact
    30781 ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg tacgcctcgg
    30841 gatcttccag cgacccaacg ggcactacca gcgccgagag cgatttaact gcctgcggat
    30901 cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac tggcggttat
    30961 ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct gcctgactta
    31021 atggtgccca gagggcaaca aacaacaata atgctttgaa ccaggtgctg cggggcattc
    31081 acggacctca taatcaactt tattttctgg acagattcaa caggttaacg caaatcatct
    31141 gccagaattt aatcttgtgc tgcacgagtc agcctatgtt tatataacca tcagtccgtg
    31201 actggtgcgc atcataaagt aagcagatag attgcgcaat ttttatacaa cactcatgac
    31261 tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat ggcctccatt
    31321 gtctctggtg ttaattgccg ccagagccgt tgataccaga tatcatcacc ttcgccacgc
    31381 accatcatgt tgccttcgta agcctgaagg aatttcgtac gggctttttg caacgtggaa
    31441 tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca cgcgccgcca
    31501 ctttcaggta acaccagcaa tggcgcggac attccttcac gatacccctc aatcagttgt
    31561 gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc accgtctttg
    31621 cgtagaaaaa ggcgactttc gccattacca ccgctggcac agtagacaag gtgttccagc
    31681 caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa caggccatcc
    31741 ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag atcaatttcc
    31801 atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg catctcctgg
    31861 cactgtgttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc tgccgcccgg
    31921 aagcggcgga acaagcgttc ggcatcatcc tgctcaacca gtgcattcaa taactgctga
    31981 ttgatttgat aacggctaag tccttccaga ataaatggct cggtgtctgg gatttcgctg
    32041 tcttcagtat ggaagttcac ctgcaaacgc atctggaaga acgcccgcac cggatgtgcc
    32101 cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg cagcggctga
    32161 acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc acgagcatag
    32221 ctttgtcgtt cgcctggctg gtagttttgc ggatcaaacg gcatccgggt atggaggcaa
    32281 gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc atcgcccggt
    32341 agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga cgggaaacgt
    32401 tcactgttat cctgaatgga acgcccgata tagctgatat agagtttttg ctgcgcggaa
    32461 attaacgctt ccaggaacag gtagcggtcg tcatcgcgac ggctacggtc gccacgcttc
    32521 ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata aacgccgtcg
    32581 ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat cagagtacaa
    32641 atgttaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag acgctgtgcc
    32701 agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc taaaccttcg
    32761 gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt ttccgcatcc
    32821 ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc tagcggacgc
    32881 tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc cagatgcccc
    32941 accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg ccactcgccc
    33001 tgcgcgctct ccatcgcgta gcccagcaac atacgcgtca ggccaaatcg ccaggtgtgt
    33061 tgtccggtgg cggggagttc cagctcgcga acgttgtcgt catctatccc ccaacgaatg
    33121 ccggattcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat gtcaaaccgc
    33181 gccgccagca ccggcacatc cagcagcgcc agcacatcct ctgacacaaa gcgactgtca
    33241 ggcagtgata acaggctgat aaacgcttcc agtaccggat gtgactgccg cgcacgacgg
    33301 tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac tgcctgaata
    33361 aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg agtaagtgtc
    33421 ggggcttcct ccagcatcgc cagcaagcga tcgtgtaaaa cttcaacttc acgctgcggg
    33481 ctatggcaaa cgtggaatgt gatactgcta tccagtggat caagcgggcg tttgttatcg
    33541 ctacgggaaa actcttcgat gttcacaccc gcaacggcgc ggttttccag ttccagaatg
    33601 tcagactgaa tgttatgcag caggttatct ggcgtcacat cgacaaaagc gtccagctcc
    33661 tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt cccccatgag
    33721 gccagcagcg agttgccgac atcctgttca ccatcgctgt taaagagctg tccggcattt
    33781 tcgctatcgc gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg ctgacgagtc
    33841 agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg gcaggggttg
    33901 gtaaacagga gatggatttc aatatgttta cccagcgcct ggagcgcctg gagataaaca
    33961 ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtaaccccgg cgggcaggtc
    34021 gtcgcggact ccagcgtttc gataaagcgc tgatagagat tcgcgcggtg ccagcgcggt
    34081 tgtccgagtt catgggtata ttccaccagc gccttccaca acggggcttg ccaggcctgt
    34141 gcttctccca gcccttcaac caaatgtcct gtttcccact gtgccagcca gtccggacga
    34201 tagaccagat actggtcaaa caggtccgcc gcttttgagg aaagctggaa cagttttcgc
    34261 ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg ctccagcaat
    34321 tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc gctctctttg
    34381 gggatttccg gtaacacccg gacgaacata tcccagataa agctcgctgg cagcggaaaa
    34441 tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca ctgcgccata
    34501 ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg ttcgcgttcg
    34561 acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta gacccttaac
    34621 atagcggctc ctgactactg acaattcggg caatgcaggc gggtcatctc gccttctctg
    34681 cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga tgtctgcatt
    34741 cggttgacct gccagttggc aggtggcgaa atcgcgcgca gttgcgtttg ctgccaacca
    34801 tgccgccaga gcttttggta ctggtttctg ctggcgagac tgttcattga tgttcgctgg
    34861 atacccgata acgcagtgac aatcatcacc atcagcacca tcagcaccat cagcaccatc
    34921 agcaccatcg caaccattac ctccggcagg ctaaagcctt gttgattctt cagggaagct
    34981 gacataatgc cccctctttc agcggacaaa aatcgctcca gccgcgtggc gaaaaaacaa
    35041 tttttccatc aatgacttcg cctgttcgcc acaacgaaac gccttcataa ccggcaatca
    35101 ataaggcttc attatctgcc agtaaacgca aacaaacctg ggcatcggtt ccagcgtact
    35161 gcgagcactg aactgctggc tgcgtctgcc aggagtgcat ttttccccac gccagcgccg
    35221 actgaacgat ggcctggcgg cgcaatgact gactttccat gctcacgcga gaggcaaaac
    35281 tgcgatcctg ctgactcatt ccttgcaata gcaagctacc caaaaccagc agcatcagga
    35341 ccagagccag tgacgaaaca cctttttcgc ggttcacagg ttgaatcctg tcacgctata
    35401 gctggcatcc accacggttt gcggttcaga cttactggca gcacgcatat taaccgtcaa
    35461 caccggcgag aagccgctga catcctgacg tacgacctga aaagtgtcga taatgatggc
    35521 atccggatta gtgactttat cccagccctt accttcacag gatgtcgcac cgcgtagcgt
    35581 ttccagcaca tgctccttca gacgaaatcc aatctggtcg gactctttta ccggttcgcg
    35641 atcccagata ccgttactgt tcgcatccca ctgcacaatg atacagtcac cctgtccgac
    35701 aatttccagc ccttcgccgg tacagctgcc atgacaataa cccgccctct ggagatgctt
    35761 cgcgacggta aatacccgca gccagatttc atcttccagt gccagcttac gagtgctcgt
    35821 taaactttca cgctgtaacg caggcagaaa gcgtgcagcc cccagcaaca atacgctact
    35881 gatcgccata gcaatcaaca cttccagcag agaaaaacct tgctctttta caggcatcct
    35941 tctgtttctc cttgctgata aagccggagt cttccccacg gcgaaaccac cagccaccac
    36001 tcgcccgttg agtttttgaa gcgaatatgc ccggcccatg cggtattgcg caggccaaag
    36061 aaagcaagcg aaggtgtcag gtcgctcatt tcgacttcgg gccagcgtgg cacaaagacc
    36121 aatggtgaac tgccatgaca ggtattggcc ccagcagcgg aactcacaag gcaccataac
    36181 gtcccctccc tgataacgct gatactgtgg tcgcggttat gccagttggc atcttcacgt
    36241 aaatagagca aatagtcccg cgcctagctg gcggtttgcc aaagccgttg cgactgctgc
    36301 cagtattgcc agccatagag tccacttgcg cttagcatga ccaaaatcag catcgcgacc
    36361 agcgtttcaa tcagcgtata accacgttgt gttttcatgc cggcagtatg gagcgaggag
    36421 aaaaaaagac gagggccagt ttctatttct tcggcgcatc ttccggacta tttacgccgt
    36481 tgcaggacgt tgcaaaattt cgggaaggcg tctcgaagaa tttaacggag ggtaaaaaaa
    36541 ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta attagatagc caccggcgct
    36601 ttaacgcccg gatgtggatc gtagccttca atctcaaagt cttcgaaacg gtagtcgaag
    36661 atggattcgg gtttacgttt gataatcaac ttcggcagcg gacgcggttc gcggcttaat
    36721 tgcagatgag tttgatccat atggttgctg tacaggtgcg tatcgccacc ggtccagaca
    36781 aaatcaccca cttccagatc gcactgctgc gccatcatat gcaccagtaa cgcgtagctg
    36841 gcaatgttga acggcaggcc gaggaagacg tcacaggagc gctgataaag ctggcaagag
    36901 agtttgccgt ctgccacata gaactggaag aatgcatggc acggtgccag cgccatttta
    36961 tccagttcgc ctacgttcca cgctgaaaca ataatgcggc gcgaatccgg gtcgtttttc
    37021 agctggttca gtaccgtagt aatctggtca atatgacgac catctggcgt tggccaggcg
    37081 cgccactgtt taccatacac tggcccgagg tcgccatttt catcggccca ttcgtcccag
    37141 atggtgacat tgttttcgtg tagataagca atgttagtat cgccctgcag gaaccacagc
    37201 agttcgtgga tgatggaacg caggtggcaa cgtttggttg tcaccagcgg gaatccatcc
    37261 tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg ttccggttcc ggtacggtcg
    37321 tttttctgtg tgccttcgtc gagcactttt tgcatcagtt ctaaatactg tttcatggtt
    37381 cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc agaccatcat gatcacaccc
    37441 gcgacaatca tcgggatgga aagaatttgc cccatgctga tgtactgcac ccaggcaccg
    37501 gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga tgcgaaacgc gccgtagcca
    37561 atcaggaaca agcctgagac agctcccatt gggcgcggtt tacgaatata caggttgagg
    37621 ataataaaca gcaccacacc ttccagcagc agttcgtaaa gctgtgatgg gtggcgcggc
    37681 agcacaccgt aagtgtcgaa aatggattgc cactgcgggt tggtttgcag cagcaaaata
    37741 tcttctgtac gggagcctgg gaacagcatg gcaaacggga agttcgggtc aacgcggccc
    37801 cacaattcac cgttaataaa gttgcccaga cgcccggcac caagaccaaa cggaatgagt
    37861 ggtgcgataa aatcagagac ctggaagaag gaacgtttag tacggcgggc gaagataatc
    37921 atcaccacga taacgccaat caggccgccg tggaaagaca tgccgccgtc ccagacacgg
    37981 aacagataca gcggatcggc cataaactgc gggaaattgt agaacagaac ataaccaata
    38041 cgtcccccga ggaagacgcc gaggaagccc gcatagagta agttttcaac ttcatttttg
    38101 gtccagccac tgcccggacg attcgcccga cgtgttgcca gccacattgc aaaaatgaac
    38161 cccaccagat acatcaggcc gtaccagtga agcgccacgg gtcctattga gaaaatgacc
    38221 ggatcaaact ccggaaaatg cagatagcta ctggtcatct gtcaccacaa gttcttgtta
    38281 tttcgctgaa agagaacagc gattgaaatg cgcgccgcag gtttcaggcg ctccaaaggt
    38341 gcgaataata gcacaagggg acctggctgg ttgccggata ccgttaaaag atatgtatat
    38401 gatccgcgct ataaccctcc gcgaatcagc ccgcccatgc cgcgacgctc cataaaggct
    38461 gcaacctgat gacgaacttc ggtcgccagt tgcgcttcca gactacgctg cgcaagattt
    38521 tctgcttcgg caaaatcaat gcgccgcagc aggtatttta cccgcgctac agaacgtccg
    38581 ttcatagaca gatggcgata cccaagccca atgaggattg ccacgcacat gggatcgccc
    38641 gccatttcac cgcacaaacg aagatcgata ccatgtattt ccgcttcccg ggcgatcatc
    38701 gccagagctc gtaacattgc aggatgaaga ctgtcgtaaa tgttcgccac ccgggtattg
    38761 ttgcgatcaa cggccagaat gtattgagtc agatcgttgg tgccaacaga gatgaaatcg
    38821 accctctttg ccagatgcgg cagcataaat accattgacg gcacttccag catgatgccg
    38881 atacgtggtt tgggaatttc gtaaccgatc atctcctcga cttcacgtcc ggcacgttca
    38941 atcaggcggc gtgcttcgtc aacttcatcg aggctggtga ccatcggcaa cagaatattc
    39001 aggttgcccg tagcggcatt agcacgcagc atcgcccgca cctggatcaa gaagatctcc
    39061 ggctgatcga gcgtaatgcg aatcccacgc caacccaggc atggattctc ttcgctgatc
    39121 ggcatgtaag gcagctgctt atctgctccg acatccagcg tacgcaaggt gacgggttta
    39181 tcattgaaca tttgcagcat cccctgatac tgcgccacct gttcttcttc cgacgggaaa
    39241 ccactttgca gcatgaatgg gatttcagtg cgataaagac cgatgccatc aatacagctg
    39301 cccagttttt cttcatgttc cgggcttaaa ccagcattga gcatgacttt tatacgctca
    39361 ccgcttttta actgcgcggg taaattgacg tcatcttccg ccagacggct aagctcaatc
    39421 tcttcactaa ttagccgctg atattcttgc agcagtaccg gctccggatc gaccagcaat
    39481 tcaccgcgat agccatcaac gatcagcgtc ctacgatgca gcaccgaagg ctgaatatcc
    39541 gcgcccatca cggtagggat ccccagcgca cgtaccatga tcgcagcatg ggagttggct
    39601 gcgccatctc gcacgacaac accgactaag cgatcctggg gcagctcagc aagagttgtc
    39661 gctgacagtt catctgccac cagaatgaaa cgttccggcc aggcgttcgg cccttgatta
    39721 gcgtcatcaa gatgaaacag caatcgctga cccagcgcac gtaaatcacc agcccgctct
    39781 ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa atttttcaat gaccgttttt
    39841 accgcccact ctgccaccga gcctttatca acctcggcaa acaattcgcg acgcagccgg
    39901 gtgtccgaaa gcaggtgcga gtaaagatcg aaaatagccg ccgtttcttt ttgtgctccg
    39961 gcggcaaagc gtttgctgta gcggcgaaac tcgtttgccg cttcttccag cgccccggtc
    40021 agtcgttcgc gttccagagc cggatccagc gttgatgcct gatacacctg ttccattaaa
    40081 ggtaacgtgg catcctgcca gccttcggca atcgccacac caggtgctgc cggtaatgcg
    40141 cggattcgcg tctggcgata ttgcccaaac agggcagtca actgcgactg agaaagaata
    40201 gctgccatct gggtggcaag cgtcaccagg aaggattctt cactttcgtc atactggcgc
    40261 aactctcgtt gctgtaccac cagtacacca agcaactggc gacgttgaat aattggtacg
    40321 cctaaaaacg cgcggaaacg ttcttctttt acggagggga tgtatttgaa gctggggtgc
    40381 ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc caaccaggcc gacgatccct
    40441 tcatcaaacg cgagcgttac agtgcgaccg cgtggttttt tcagcccccg ggtcgccatc
    40501 aggtagtaac aacgtcgatc atgatcggcc aggtagaccg aacagacctc ggtatccatc
    40561 gcaagacaga tgtcggtaac cagaatattt aacgcctcat tcaggcgtgg tgcgctggct
    40621 accttttcga ctatttcgcg caggcgagtg agcataattg gcgtgactta acctctttta
    40681 cgtcgataag cagatgcgtt ttgtggtttt ggcgtatttt cctgcagtga catcaccaca
    40741 ctcgcgaact ctttcattac cctacggtag acatcacgtt taaatgacac cacctgtctg
    40801 accggatacc agtaacttac ccatcgccag ccgtcaaact ctggtgtact gctggtttgc
    40861 atattgattt ctgcatcgcc gcttaccagc tgcaagagaa accatttttg tttttggccg
    40921 atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg gtaatttgta gcgcaaccag
    40981 ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta atcctacttc ttcaaacaat
    41041 tcacggtaca tcgcctgctc tgcggattct ccggggttga ttccgccttg cggaaattgc
    41101 caggagtgct gaccaaatcg ccgggcccac attacctgcc cctggcgatt acaaatcacg
    41161 atacctacgt ttgggcggta gccatcgtca tcaatcaccg gactacctca aaataaagct
    41221 ttatatacga atgattgttt catactccag gaagacggta aaccactctc tgcagggcat
    41281 tacacactaa taacaattga ataactcaca gttatgtgca gagttataaa cagaggaagg
    41341 ggtggatagc cgtttttatt caccttttct gtggatagag ttgtgaagaa ctacggaatt
    41401 actacgggaa aacccggagc attctgaata agcccgatat agacatttta aaatattcat
    41461 tattagtcaa tgcattacat gtaatttgag taatgaaaaa gagtaattcg tgacccaggt
    41521 cacacctctc atttacgggt tggcgaaaga tcaaccaatg ccgtgtttat ccacagaatg
    41581 tgccactaag ttaagcactg aaccactaaa aactggagtt tcgtcgcacg tcaaggctgt
    41641 aaatgaaaac agtagtggag gtttttcaca gttatcccag ctttctgtgg ataacatggt
    41701 gtaagatcct gtttattttc agtgaccaga tttggaaaac ctattgcagt gttgcgcaac
    41761 tcgattaccg gcagcctaaa aagcaatata aatcagagaa ttgaacaaca catgtggaaa
    41821 aagttacact gcgaatattc ggcacataat tgctgtttgt tttttaacca aggtatcatg
    41881 acatgtccca acctcgccca ctgctctctc ctcccgaaac tgaagaacag ttgttagcgc
    41941 aagcacagca actttctggt tatacattgg gagaactggc ggcacttgac gggctggtta
    42001 cgccagagaa tttaaaacgc gataagggct ggattggcgt gttactggag atctggctag
    42061 gtgccagcgc agggagtaaa cctgagcaag attttgctgc tctgggcgtg gaacttaaaa
    42121 ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac attcgtttgt gttgcccggt
    42181 taacgggtaa tagcggggtg acctgggaaa ccagccatgt gcgccacaag ctcaaacgcg
    42241 tactgtggat accggttgaa ggcgagcgca gcatcccgct ggcgcagcgt cgcgtaggat
    42301 caccgttgct gtggagcccg aatgaagagg aagaccgaca gctacgcgaa gactgggaag
    42361 aattaatgga tatgattgtt ctcggtcagg ttgagcggat caccgctcgt cacggggaat
    42421 atttacagat aagaccgaaa gcagcgaatg cgaaagcgct taccgaagcc attggtgccc
    42481 ggggcgaacg gattctgacg ctgccacgcg gcttttattt gaagaagaat ttcaccagtg
    42541 cactactggc ccgtcatttt ctgatccagt agccatcgct ttgacctgcc gctttccggg
    42601 catataatta ccgcttcatt tttttggcag ggctttttag atgttatttg catggataac
    42661 cgatcctaac gcttggcttg cgctcggtac gctgacgctg ctggagatcg ttcttgggat
    42721 cgacaatatt attttccttt ctctggtggt ggcaaagctt cccacagcac aacgtgctca
    42781 tgcgcgccgt ctggggttgg cgggagccat ggttatgcgt ctggcgctgc tggcatcaat
    42841 ctcttgggtt acgcgcctga caaatccgct ttttacaata ttcagtcagg aaatttctgc
    42901 ccgtgatttg attctgcttc tgggtggctt gttccttatc tggaaagcca gcaaggaaat
    42961 ccacgaatcc attgaaggtg aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg
    43021 cgctatcgtg cagattatgc tgctggatat tatcttcagc ctcgactcgg tgattaccgc
    43081 tgtgggtctg tcagatcacc tgtttattat gatggcagcc gtggtgattg ccgtaggcgt
    43141 gatgatgttc gccgcgcgct cgattggtga ttttgtcgaa cgccatcctt cggtaaaaat
    43201 gctggcgctc tctttcctga ttctggtggg ctttaccctg attctggaaa gtttcgacat
    43261 ccacgtaccg aaaggttaca tctacttcgc gatgttcttc tctattgcgg ttgaaagcct
    43321 caacttgatt cgcaacaaaa agaatccgct ctgatactcc gtacgctctc ctgcgggagg
    43381 gcgtactcac tccttttacc ctcacagttt aagattttcc tgctttcaag atatataacg
    43441 tcggtttata aacagactat tatcataggt aggccaaatg aggataaccg gatgaaaaaa
    43501 tgggcagtaa taatttctgc agtcggactg gcgtttgctg tttccgggtg ttccagtgat
    43561 tacgtcatgg cgaccaaaga tggccgtatg attttgaccg atggaaaacc tgaaattgat
    43621 gatgataccg ggctggtgag ttatcacgat cagcaaggta acgcgatgca aattaaccgt
    43681 gatgatgttt cgcaaattat tgaacgttaa caaataaggt cagcatccgg ctggccttaa
    43741 gatttttctc tttccctttt cccttccctc tgccattttt atattcctta tgtcgtgatt
    43801 ataaaaagga aacggctatg caatatcacc gtatacccca cagttcgctg gaagtcagca
    43861 cgctggggct tggcacgatg acgtttggtg aacagaacag cgaagccgac gcccacgcac
    43921 aactcgacta tgccgtcgct cagggcatta accttatcga cgttgccgaa atgtacccag
    43981 tacctccgcg ccccgaaacg caagggttaa ccgaaaccta cgtcggcaac tggctggcga
    44041 aacatggcag ccgcgaaaag ttaattatcg cctccaaagt gagcggaccg tcgcgcaata
    44101 atgacaaggg catccgcccg gatcaggcgc tggatcggaa gaatatccgc gaagcgctgc
    44161 atgacagcct caagcgccta cagactgatt acctcgatct ttatcaggtg cactggccgc
    44221 agcgcccgac caactgcttc ggcaaactcg gttatagctg gacggattct gcgcctgcgg
    44281 tttcgctgct ggatacgctg gacgcactgg cagagtacca acgcgcggga aaaattcgtt
    44341 atatcggcgt gtcgaacgaa actgcatttg gcgtaatgcg ctacctgcat ctggcggaca
    44401 aacacgatct gccgcgtatt gtcaccattc agaaccctta cagtctgtta aaccgcagtt
    44461 ttgaagtagg tctggcagaa gtcagccagt atgaaggggt cgaactgctg gcctattcgt
    44521 gcctgggttt cggcacgctg accgggaaat atctcaatgg tgcaaaaccc gctggcgcac
    44581 gtaatacgct ctttagtcgg ttcacccgct atagcggtga gcaaacgcaa aaagccgtcg
    44641 cggcgtatgt tgatatcgcc agacgtcatg gccttgatcc tgctcagatg gcgctcgcgt
    44701 ttgtacgccg tcaaccgttt gttgccagca ctctgctggg cgcaaccacg atggatcagc
    44761 tgaaaactaa catcgaaagt ttgcatctgg agttaagcga agacgtatta gctgaaattg
    44821 aagcggtaca tcaggtttat acttatcctg caccataaag attaacgccg gttaaaaccg
    44881 gcgttaaata ttaatgacgg cgctgccaga tccacagcgc cgttattgcc agcgcaaata
    44941 gcgcaccgaa gccaatgcca atgggcacga ccgggatgcc taccattacc gccagcgagt
    45001 aaatgcccag catcaacaac atggcgctgt tttcgccaag gttttgtact gcaatcgcat
    45061 tccccgcccc gacgcttttt ttaccccgct cctgtagcaa cgcattgagc ggaacgacaa
    45121 aaaagccccc cagcacgcca atcagcatca acaaggcata ggctggcagc agctcgtgtt
    45181 gcagggagaa aatcggtacc accacgccaa tcaaaatccc ggctggcata cagcgtgaca
    45241 cggtttccag cgtaaccagc ttcgctgccg cacctgcgcc aaccacgata ccaatcgcta
    45301 ccatcgcgtt gagataggtc ggcgtagcgt tatcggtaat gcccagcgcc accggtaccc
    45361 acagcaccaa caggaaacgc agcgtgacac ccgctcccca gaataaactg gtgcccacca
    45421 gcgaaaaacg cgtttcacca ttgcaccata gcgaggtgca ggcattcagg aaactgcggg
    45481 tcatgttgat gagattccag gactgccccg gacgcgccgc cagtttggga atgtagatat
    45541 tggcaacgac cgcaccaccg taggccagtg cgcatgcggc cagagcgacc aggacatgcc
    45601 agtcagccag cacaccaccg gctacggaac cgagtaaaat cgccgctatg gcagaagctt
    45661 ccattaaacc gttagctttt actaacttac tacccgtggt taattcgccg agaataccgt
    45721 atttcgccgg tgaataggct gcagcaccaa cacccaccag cgtatagccg agaaacggat
    45781 tgataccaaa gcagatactg gctgcgccca gcagcttcag gccgttggca aacatcatca
    45841 cccggccttt ggcgaagcta tccgccacct gcccgacaaa cggcgccaaa agaatgtaag
    45901 cacctacaaa caccatttgc aggatgggct ggctccactc cggatagaac tgcgctttca
    45961 gtaacgccag agtggcaaac agtagcgcgt tatcgccaaa cgcagagaga aactgcgcca
    46021 cgataaccgc tttcatcccc ttcgaccaca acgaagtgtt agtgtgcact gactcactca
    46081 tcgtgttgtt ccgcttcgtc tacccagctt ttcaacgtga caaagtcagg tttgccgctg
    46141 ccaagtaatg gcatctgttt cagatagcga atatcgcgcg gtacagcaag ctccggcacg
    46201 ccgtgctcgc gggcatactg ttgcaactta tcgcgcgtca gttcgttatc tgtggtgaaa
    46261 agcaccagtg cctcgccttt gctggcatcg ctcttaatcg cagtggcatg gactttatct
    46321 ggcgaaacac caagtgccag ttgttccacc atttccagcg acaccatttc gcctgcaatt
    46381 ttggcaaagc gttttgcgcg gccctgaatc tgcacaaagc cctgctcgtc aaaacgcaca
    46441 atatcgccag tgtcatacca gccgcgctcc atttcgccgc gaacattctc ggcggtgggc
    46501 acttccagta cacctggctt ctccacccgc agatagccgt tcattatgtt cggccctttc
    46561 agttgcaggc gtccgccctc ttcgatacca gggaccgaca acaggcgtgc atccattcct
    46621 ggtagaatac gccctaccgt accgggtttc gccgccatcg gtacgttgat agaaacgaca
    46681 ggcgcgcatt cggtcacacc gtagccttca aggatgcgca ggccaaattt atcctgccaa
    46741 agctgtttgg tactttcttg tagtttttct gcgcctgcca ccacatagcg tagacgatag
    46801 aagtcatacg ggttggcgaa acgcgcgtag tgaccgagga aagtcgaggt accgaacaac
    46861 acggtgcaac tgcggtcata caccaagtcc ggcacaatgc ggtaatgcag cgggcttgga
    46921 taaaggaaca cttctgcacc tgtaagcagt ggcgtaaaca ggcctaccgt cagcccaaag
    46981 gagtgaaaca gcggtaatgc cgacataaag cgatcgttgg tggtgaagtc ggcaatcgtt
    47041 ttaatctgct cgacgttcgc cagaatgctt ttatggctat ggacgacgcc ttttggatgg
    47101 ccttcagaac cggaggtaaa gaggatcagc gcctcttctt ccggctgctg ttttacctgc
    47161 gccaggcgcg gcatcagcaa atgagcgaag atccatactt tgtcggcagt ggtgacatct
    47221 gcttttaaat cttccagata gacccagcgc acctgagtaa gttgctccgg cagatgccag
    47281 agtttgcctt tatcgagaaa ctggcgggaa gtgaagatgg ttttgatttc agccgccgta
    47341 atagcactgg tcagcccttt taccccggca gtgtagttca tcattgcggg aatgcggcga
    47401 cgcgcgatgg ccccaaaaat cactgccgca ctgatgcctg cattgggcag cattaagccg
    47461 atgcgttcgc cttcaacact gtatttttca aggatgcgtc caacaaacag cgttttcgta
    47521 agcaatttgc gataggagtc tggggtaaag ttgacgtctt cgacacattt cttcccggct
    47581 ccgaagcggt acattgcact cagtaaagat tcgtacagag tttcacgcgg gcgcaccgcc
    47641 atacgcgctt ccatcattat ttgatgcagc atttcgccag cgattttgcg acggtcacgg
    47701 gcacgcggcg catccggcat cgccacctgc gttggaggca aaatatgcag agtaatttgc
    47761 gggaacaagc gacgtttaac cagacctttc aggcggctga agtgcgtaag ttccgcccct
    47821 tcaatacgca caggaataac cgttgcacca gacttcgccg cgacaaaacc cgcgccatcg
    47881 tagattttca tcagcgagcc tgtagtggtg atgcgtcctt cagggaaaat caccactggt
    47941 cggccctgtt ccaccagacg taccagatgt ttaatagcca taggttgcgt cgggtcgaga
    48001 ggaacaaagt cgataaatga tttcagccaa cgcatatacc actgttggct tattgaggtg
    48061 taaacggcaa acactggacg cacaggtaaa aacagtccaa gcaaaatgcc atcaataaaa
    48121 gagacgtgat taggcgtaat tagtacgcgc tcgcccttca gtgcctgggt gtcacccgta
    48181 acgcgaacgc gatacaaaac acggcacaaa tttcgaaaaa agctaaaaag cattccaact
    48241 ccctttgctc tgattcagta aaagcgaatg gagggagatt acacgagata aagaacgcga
    48301 gcgacagtaa attaggtgcg aaaaaaaacc tgcgcatccg cgcaggttgg tgcaagagac
    48361 agggtacgaa gagcgtaccg aataatctca ccaatcaata cctctgggat cttgattgtg
    48421 gtctgcacga cgactcttcg ccagcgagaa aacgcaaagg aatgaaggga aatgcaacga
    48481 ggtgtgtaaa ttgtcggtta ctgttacaga ttgatgaccg gcaaaaaaaa acctgcgcat
    48541 ctgcgcaggc tggtgtaatt catgtgctca acacgaagtt gacttcacct atcaatacct
    48601 ctgggatcac cactttagca acctgaagcc aaacgccacc agcggtcaat cgcaacagcg
    48661 tttcgcaaag tgtaaccaaa ggtttgcaat ctcttttttt gtcttactta tttgcgtttg
    48721 gctcacattc ccacgatgaa aacacgccac cccttgaacc aacgggcgtt ttccgtaaca
    48781 ctgaaacaat gtaagcgttt acccactaag gtattttcat ggcgaccata aaggatgtag
    48841 cccgactggc aggcgtttca gtcgccaccg tttcccgcgt cattaataat tcacccaaag
    48901 ccagcgaagc ttcccggctg gctgtgcata gtgcaatgga gtctcttagc tatcacccga
    48961 acgccaacgc ccgtgcgctg gcgcagcaga ccactgaaac ggtcggtctg atcgttggtg
    49021 atgtttccga tccgtttttc ggtgcaatgg tgaaagcggt cgaacaggtg gcttatcaca
    49081 ccggtaattt cttattgatt ggcaacggtt accacaacga acaaaaagag cgtcaggcta
    49141 ttgagcaact gatccgccat cgctgtgctg cgttggtcgt ccatgccaaa atgatcccag
    49201 atgccgattt agcctcatta atgaaacaaa tgcccggtat ggtgctgatc aaccgtatcc
    49261 tgcctggctt tgaaaaccgt tgtattgctc ttgacgatcg ttacggtgcc tggctggcaa
    49321 cgcgtcattt aattcagcaa ggtcataccc gcattggtta tctgtgctct aaccactcta
    49381 tttctgacgc cgaagatcgt ctgcaagggt attacgatgc ccttgctgaa agtggtattc
    49441 cggccaatga tcggctggtg acatttggcg aaccagacga aagcggcggc gaacaggcaa
    49501 tgaccgagct tttgggacga ggaagaaatt tcactgcggt agcctgttat aacgattcaa
    49561 tggcggcggg cgcgatgggc gttctcaatg ataatggtat tgatgtaccg ggtgagattt
    49621 cgttaattgg ctttgatgat gtgctggtgt cacgctatgt gcgtccgcgc ctgaccaccg
    49681 tgcgttaccc aatcgtaacg atggcgaccc aggctgccga actggctttg gcactggcgg
    49741 ataatcgccc tctcccggaa attactaatg tatttagtcc gacgctggta cgtcgtcatt
    49801 cagtgtcaac tccgtcgctg gaggcaagtc atcatgcaac cagcgactaa ccgcagttaa
    49861 agcaattcca gcgccagtaa ttcttcgatg gtctggcgac ggcgaatcaa ccgcgcctga
    49921 ccattatcaa acagaacttc tggtaacagc ggacggctat tgtagttgga tgacattgat
    49981 gcgccatatg cccctgtatc atgcagtacc agataatcac ctgccttcac ttccggcaag
    50041 gcgcgggttt caacatttcc cccttcctgc tgggtaaaga catcgcccga ttcacataac
    50101 ggcccggcga cgacggtttc caccgttggc gcgtgttcca gagaacgacc atcagctgcc
    50161 agggcactga tatggtggta actaccgtac attgccgggc gcatcagatc gttgaacccg
    50221 gcatcaacca gcacaaagtg gcggctaccc atttgtttga cgctccgcac ctgagtaatt
    50281 aatacgccag actgcgctac caggaagcga cccggttcaa tttccagttt cacagggtgg
    50341 cccaaatggc gagcgatttg ctcacgcgcg gcattccaca gaccataata atgttcggta
    50401 tcaaccgcct cttcaccctg ttgataagga atagaaagcc caccgcccgc agaaatagcc
    50461 tgtaaatcct gaccgaattc gataacctga cgcaccatag caccacacac ctgctccaga
    50521 tgggcataat caacgccaga accaatgtgc atgtgaatgc cgactagctg cagatgatga
    50581 cgttgtatca cgtccagtgc ggcgggcaga tcggtgtacc agataccgtg cttgctgttt
    50641 tcgccaccgg tattggtttt ttggctatgt ccgtgaccaa accccggatt aacgcgcagc
    50701 catacccgat gccctggcga aacctggccc agttggtcga gcgtatcaac agaacccgca
    50761 ttcaccggaa tttgcaattc actgacgcgt tcaagcgtcg cctgatcgat aacatctgcc
    50821 gtaaaaacaa tatcatcggg gtgcgtttgc ggattgtaac ccgccgccaa cgcacgctct
    50881 atttcgccta acgagacgga atccactttc acgccctgct cacgcattaa gcgcaaaata
    50941 tgaatattgg aacaggcttt ctgtgcaaag cgcaccactt caaactgttt cagcgctgca
    51001 atctgccgac gaataatttg cgcatcgtag acccacaccg ggcagccaaa ttcagcgggc
    51061 aaacgcagca gattttcggc ggtgagatcg gtatcggtgc tgaacagtga atgtggcata
    51121 acaaactcca gatgagtgct tttttatgat tacgccacat cataaaaaga ataaaaaata
    51181 tcgatttatg tcgagtctat gcaaaaatga tatgaattac cggattgcga gagagcgcta
    51241 atggccgccg ttaacttacg tcatattgaa atttttcatg cggtaatgac cgccggaagc
    51301 ctgactgagg cagcacacct gctacacacc tcacagccaa ccgtcagccg cgaactggcg
    51361 cgctttgaga aggtgatcgg gctgaaattg tttgagcgcg tacgtggacg attacatcct
    51421 accgtgcaag gactgcgtct gtttgaagaa gtgcaacgat cctggtacgg actggatcgc
    51481 attgtcagcg ccgcagaaag tctgcgcgag tttcgccagg gagaactgtc tattgcctgc
    51541 ctgccggtct tttcgcaatc ttttttaccg cagctcctgc aaccctttct ggcacgttat
    51601 cccgatgtca gcttaaatat cgtgccccag gaatcaccgc tacttgaaga gtggctctcg
    51661 gcccagcgtc atgatttagg actcactgag acactccata cgcctacggg aacagaacgt
    51721 accgaattac tctctttaga tgaagtgtgt gtgttacctc cgggtcatcc gctggcggta
    51781 aaaaaggtat taacgccgga tgattttcag ggtgagaatt acatcagcct ttcccgtact
    51841 gacagctatc gccagttgct ggatcagcta tttactgaac atcaggttaa acgacgcatg
    51901 atcgtagaaa cccacagcgc cgcgtcagtc tgcgcaatgg tacgggcggg ggtaggtatt
    51961 tcggtggtta acccgctcac cgcactagat tatgcggcaa gcggtttagt ggtgcggcgg
    52021 ttcagtattg cggttccgtt caccgtcagc ctgatccgcc ccctgcaccg cccgtcatca
    52081 gcgctggttc aggcgtttag tgggcattta caagcggggt taccgaaact ggtcacttct
    52141 cttgacgcta ttttgtcgtc agctacgaca gcataaaagc gacagcatcc tcggcatgga
    52201 tcgccgcggt atcaaacaca ggcaaaacac tgcgctcttc tggcaccagt aaaccaattt
    52261 ctgtgcagcc aaaaatgacg ccctgtgcgc cctgttctgc aaggcgagca atcacttgcg
    52321 cataataagc gcgtgacgct tcggtaaatt gccccagaca cagttcttca aaaataatct
    52381 gattaatttt cgcccgttca tccgcttcag gaataagaca attgatggaa aattgttccg
    52441 tcagccgccc gcgataaaaa tcctgttcca tggtgtaacg cgtacccagc agcgccacac
    52501 gagtcattcc ggccccggta attgcacgtc cggtggcatc cgcaatgtgt aagaaaggca
    52561 gagtgcaacg tgactcaatg gcatccgcca ctttatgcat cgtattggta cataacacaa
    52621 taccttctgc gcccgcccgc tgtaagccaa gcgccgcctc agccagaatg tccccggttt
    52681 tatcccattc cccgcgacgc tggcactctt ctatttcatg aaaatcgacg ctatgtagca
    52741 gcatttgcgc agagtgaagc ccaccaagcc gctgtttaat gccttcattt atcagacgat
    52801 agtaaggaat ggtggattcc cagctcattc ctcccagcaa accaattgtt ttcattcctc
    52861 tctcctgatg tgtgttaccg caacaaggct gaataaggaa cgagacaaac gcctcaacgg
    52921 ccaggtgccc aatctctatt aacgaaaaaa gggccggatg tacagcacat ccggcccgtg
    52981 aaatcagacg ccgatatttc tcaacttctc gcctgccatc agtttgcgtt cgatatgttc
    53041 cagcgtgaca tttttggttt ccggaatgag ccagaaagta atacccacaa acgcaatgtt
    53101 cagcgcagtg tagagccaga acgtaccggc agcgccaatg ctatcaagca gtgtcaggaa
    53161 ggccgcgccg ataatcatat tcgacaccca gtttgtcgtc gtcgaacagg taataccgaa
    53221 atcgcggcat ttcagcggct gaatttcaga gcacaggatc cacaccactg gcgcggcgct
    53281 catcgcataa ccggcaatac acatcatcgt catgccaaca gagagccagg acaagccact
    53341 ggaagccgta ccgttatcaa actgcatcag gcaatagccc agcaccagag tgcctaacgc
    53401 catcacgctg aaaccaattt tcagagccgg tttacgacct gctttatcta ccgtaaagac
    53461 cgcaatgaag gtcgcgaaca taaaggtcag tcccacgacc agagtcgcaa tcatctgttg
    53521 ttctgtggtc gtaaagcccg ccattttgaa gatacgcggc gcgtagtaca tgatgatgtt
    53581 cataccggta aactgctgca tcgcctgcaa caacataccg agaaacacag cacgacggac
    53641 gttacggttg atcttaaaca gtgcccaacc gccctgtttt aacttcaggc tttcacgaat
    53701 ttcgttgagt tcttctcgcg ctttttccga cgtatcgcgc agcatacgca acacttcttc
    53761 cgcctcaata tgacgcccct tttccgccag ccagcgcggg ctatttggca ggaagaccac
    53821 cagaataatc agcagaactg ctggtaaagc aagaaccccc aacattgcgc gccagttacc
    53881 gctataactg aacgctgtat cggataaaaa cgccagcacg atgccgagtg tgaccatcaa
    53941 ctggtacata ctgatcatct taccgcgaac gttttcactt gccatttcag aaagatacag
    54001 aggagcggtg taagacgcga tcccgacagc aatgcccagc accacacgag cggcgattaa
    54061 catctctacg ctggtcgcaa aagcggaccc tatagaaccg agtacaaaca ggatggcccc
    54121 cgccatcagg ctgtatttac gccccaggcg gaacgacagc caaccattaa acagcgcacc
    54181 aattgctgcg ccgagcatca tgctactaac cacccattcc tgcaaacgac tggtcagcac
    54241 aaagtgatcg gtaatgaacg gcaacgctcc ggcgattacg ccgatatcaa gaccaaataa
    54301 caatcctgcg accgcagcag ctaccgaaac aaacatattc atacgccgcg tatcacgcaa
    54361 agaacgtggc gttaaagcag attccgtatt gatagtaacc attttttcct gccagcagag
    54421 agtaagacat tgtgaaaaaa tacgtgaaca attcacacag gtgtcaggtc ggaaacagca
    54481 taaatatgga ttaaattgct gcgacatgcc gttatgtgat ggatattcca attttaaaat
    54541 taagttgaat tattgagatt attattaacc tcctaatttt aaagcagata aaattcataa
    54601 agttcattaa ttgataattg atatggatta tttcataacc atgatatgga ttatgatgat
    54661 ctagagatat aaaaaaccct gccatgcggc agggtcataa aagtaagagg aatgaattaa
    54721 cgcgccagcc aaccgccatc cacagcaatg gtataaccat ttacataatc tgaagcgctg
    54781 gaggcaagaa acactaccgg ccccatcagg tcactcggca gtccccaacg gccagctgga
    54841 atgcgatcga gaatttccgc gctacgttgt tcatctgccc gcagttgttg ggtattgttg
    54901 gtcgccatgt aacccggagc tatcgcatta acattaatgt tgtgtttagc ccattcgttt
    54961 gccatcaatc gcgtcacacc catcacgccg ctttttgatg cggtataaga aggcacacgg
    55021 atcccgccct ggaaggagag cattgacgcg atattgataa tcttgccgcc attgccttgc
    55081 gcgataaagt gtttcgccgc tgcctgagac atgaagaata cgctcttgat attcaggttc
    55141 atgacatcgt cccagtcttt ttcgctgaac tcgagagcgt cttcgcggcg aatcaatccg
    55201 gcgttattca ccaggatatc aatatgacca aattcagcta ccgcgcgatc cagcagtcca
    55261 ggaatgccat caatctttcg cagatcggcg gtcaggctta aaaaacgacg ccccagcgcc
    55321 gtgacctgct cgatggtttc agtcggttca acgatgttaa tgccaacaat gtcacagccc
    55381 gcttgcgcca gccccaacgc catcccctgg cccagcccag tatcacaacc agtgacgacc
    55441 gcaactttac cttggagaga aaatgcactt aaaatcataa caacacctta ttcgtttatg
    55501 cccacaacta gcgcaaatct ttaacgccca catggtccat atcattaaag acctggtttt
    55561 caccgaccat gccccagata aaggtataag ctttggttcc gacgccggaa tggatcgacc
    55621 agctcgggga gatcaccgcc tgctcgttat gcatcacaat gtgacgcgtt tcttgcggct
    55681 gccccatcat gtggaaaacg caggcgtcat catccatatt gaaatagaaa taaacttcca
    55741 tccggcgctc gtgggtgtga cacggcatgg tgttccacaa gttacctgga gccagctccg
    55801 tcagccccat actcaattga caggtttcca gtacatccgg gacaaaatat ttgttaatcg
    55861 tgcgacggtt actggtgagg ttatcgccta acgtgactgg agatacttcg tccggtgtga
    55921 cttttttggt gggatacgtc gtatgcgcgg gtgcgcaatt gtaataaaac ttcgccggag
    55981 tggcggtatc aatactggca aagacaactt cttttgcacc tttaccaaca tacagggcgt
    56041 cgcggtgacc gatttcatag cattgaccat cgacagtaat cgtaccggca ccgccaatat
    56101 tgataacacc taactcgcga cgttcaagga aatagcttac gccgagttgt ttaccaactt
    56161 ccccgccaac ggaaaccgtt ttagttatcg gcataatgcc gccaacaata attcggtcaa
    56221 tgtggctgta aaccatggtg tactcatcgg cgacaaatac cttttcaacc aaaaattcat
    56281 tgcgcagccc ttgggtatcc agcgtttttg cgtgcgcacc gtggatgctc tgtcttacgt
    56341 ccacatcaac ctccgataat cagtgaagcg tgaaaataga aaacgatagt gaaacactgt
    56401 ttcgttttct ctataaaggg atgatatgcc catgggctag ggttttcaat taaaaataaa
    56461 acaatgtttt atttttaatt taaggattaa aaaaagtgca gtgtcgatca cgaatatgtc
    56521 atgagaatgg aaaaatgagg gagcccagaa aatgtatgtc gacttgccag ccatcattat
    56581 tcaacagatt aatgacggca gatatttatc ggtaaggagg aaatgcagag ctactcgtca
    56641 cgttcaatgg tcaatgccac gccctggccc ccgccgatac aaagcgttgc cagccctttg
    56701 cgggcattac gtttcaccat ttcatgaacc agagaaacca ggattcggca accggaagcg
    56761 cctatcgggt gaccgagtgc gatcgcgcca ccattgacat tgacccgacg ctcatcccac
    56821 tcaagcatct tgccaaccga aagcgcctgt gcagcaaacg cttcattagc ctcgataaga
    56881 tcgacatcag ccaactgcca gcctacacgc tccaggcaac ggcgggtcgc atacaccggc
    56941 gcaattccca tcaatgccgg atctacacca acgctggcaa atgcgcggat gcgggccagc
    57001 acgggtaaat tcaacgctcg tgctttggct tcgctcatca tcattaccgc agctgcgcca
    57061 tcgtttatgg atgatgcatt ccccgcggtc acagaaccga gactatcaaa tgaaggattt
    57121 aaacgggcta aaccttctgc actggcgtca gtgcgtggct gttcatcggt atcaacaacc
    57181 aacgtctgac cgttactttg gatcattacc gggacgatct catctttaaa tcgtccggcg
    57241 tcaatcgccg ctcgcgcttt ttgttgcgag ctaagtgcgt aagcatcctg caactgacgg
    57301 ctgatgccat attcgcgagc cagattttcg gcggtgacac caatatgata atcattgaag
    57361 gcatcccaca acccatcatg caccagactg tcaaccaact ggctattgcc aagctgttca
    57421 ccggtgcggc tatcagtcag aacatgtggt gcgcggctca tgttttcctg gccacctgcg
    57481 atgacaatat cagcctcgcc acactgtatc gcctgagtag ccagatgcag tgctttaaga
    57541 ccggaaccgc aaacgtcatt aatcgtgatt gcagaaacgc tgttaggaag accaccttta
    57601 atagccgatt gccttgccgg attctgccct gcccctgcag tcaacacctg accaagaatt
    57661 acttcatcca ccgcatatgc aggaacgccg gtacgttcta ttaacgcttt cacgaccaga
    57721 ctaccaagtt ccacggcgga atgacccgct aacgcaccac gaaagcagcc gataggtgtc
    57781 cgtaacgccc cgacaatcac aacgtctttc atcacaacct cgcaccaatt aacagtgcaa
    57841 tagtaaatga ttgttaatta cagttatctt aattgtttaa aaaaagtgat ttttatcaca
    57901 aaggaaatat gcctgagcag tagtcagagt cataactggc acgtaaggtt tgcaaccact
    57961 aacccaccaa tagatgggta gatagggcgt taatctccca tacttaactt ggtttatggt
    58021 aaattgccct ccattttgtt taatttgtag atgatacgtt cagataatgt ctaatatttg
    58081 gtctaaagaa gaaactctgt gaagtttcgc gctctatggc acagccgttg atgcaggcac
    58141 gctattcctt cctattcagt taggttcggc aggggctgtg gtcctgttta ttactgctct
    58201 ggtcgcctgg cctttaacat attggccaca taaagcctta tgccagttca tcctctcatc
    58261 aaaaacatca gcaggtgaag ggataacggg cgcggtaaca cactactatg gcaagaagat
    58321 tggtaatctg attaccacgc tgtacttcat cgcctttttt gtcgtcgtgt tgatatatgc
    58381 agtggcaatt accaactcac ttacggaaca gctggcaaag catatggtta ttgatcttcg
    58441 catccgtatg ttggtgagtc tgggtgttgt attaattctg aatctcattt ttctgatggg
    58501 acgtcatgcc actattcggg taatgggatt tttggtattc ccattgattg cctatttctt
    58561 atttctttcc atttaccttg tcggtagttg gcaacctgat ctattaacaa cccaggtaga
    58621 gttcaatcag aatacccttc accagatatg gatatcgatt cccgtgatgg ttttcgcctt
    58681 tagccatacg cccattattt ctacgtttgc catagacaga cgtgaaaaat atggcgaaca
    58741 cgctatggat aaatgcaaaa aaattatgaa agtcgcttat ctcatcatct gcataagtgt
    58801 actgttcttt gtctttagct gcctgctttc tattccatct tcgtatattg aagcggctaa
    58861 agaagaaggg gtcaccattt tatcggcgct ttctatgctg ccgaacgccc cagcatggtt
    58921 gtcaatttcc gggattattg tcgcagtagt tgcgatgtcg aaatcattcc tgggtacgta
    58981 ctttggcgtt attgaaggtg ccacagaggt cgtcaaaaca acactacagc aggttggtgt
    59041 aaagaaaagt cgtgcattta accacgcact atcaattatg ttggtatcgc tgattacctt
    59101 cattgtttgt tgcattaacc cgaacgcgat ttcgatgatt tacgcgatca gcggcccgct
    59161 cattgccatg atacttttca tcatgcctac gctgtcaacg tatctcatcc cggcgcttaa
    59221 accctggcgt tccatcggaa atctgattac gctgatcgtg ggtatcctgt gcgtatcggt
    59281 aatgttcttt agctaatatc cgttgtggat ggttcaaaat tgctacaatt ttgaaccatt
    59341 ccatttttca taatattcat ttgttattat tccgccctat cacttctctc gtcatatcct
    59401 agaattatca gttagttaaa cacacaaaga gatctgaaca tcttaaataa gactatttaa
    59461 gatgcataac ttagattcgc aagatatctt ctggtcattc aaaaacaatt tgcgatatag
    59521 atattctcat accatgagat agttatctgg accacgttcg accatgcaag attaaatcag
    59581 tgaaacataa tcatatttaa taacgagaaa tgcattttta aatatcttaa tcagaattat
    59641 agcaaataca gattaatcgc aatttttccc agcaaaatta caaaatcaag gtgtattgac
    59701 atcatcaatc accaatgaaa ttatgcaatt atatacggat agggaggttc ttaacatggg
    59761 gctttgtagt cgttataaaa gtcttacatg caatagttgc tcaatgcatt gccaaataat
    59821 gccagaagag tcaccgcgtt tgcagtactg cgcaaactcg tgttttgatc ttacccagca
    59881 atagtggaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac atgacaaaaa
    59941 cagtatcaac cagtaaaaaa ccccgtaaac agcattcgcc tgaatttcgc agtgaagccc
    60001 tgaagcttgc tgaacgcatc ggtgttactg ccgcagcccg tgaactcagc ctgtatgaat
    60061 cacagctcta caactggcgc agtaaacagc aaaatcagca gacgtcttct gaacgtgaac
    60121 tggagatgtc taccgagatt gcacgtctca aacgccagct ggcagaacgg gatgaagagc
    60181 tggctatcct ccaaaaggcc gcgacatact tcgcgaagcg cctgaaatga agtatgtctt
    60241 tattgaaaaa catcaggctg agttcagcat caaagcaatg tgccgcgtgc tccgggtggc
    60301 ccgcagcggc tggtatacgt ggtgtcagcg gcggacaagg ataagcacgc gtcagcagtt
    60361 ccgccaacac tgcgacagcg ttgtcctcgc ggcttttacc cggtcaaaac agcgttacgg
    60421 tgccccacgc ctgacggatg aactgcgtgc tcagggttac ccctttaacg taaaaaccgt
    60481 ggcggcaagc ctgcgccgtc agggactgag ggcaaaggcc tcccggaagt tcagcccggt
    60541 cagctaccgc gcacacggcc tgcctgtgtc agaaaat
//
LOCUS       DAJTRD010000013        60419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTRD010000013 DAJTRD010000000
VERSION     DAJTRD010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.013"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..322
                     /locus_tag="OO315_000889"
     CDS             <1..322
                     /locus_tag="OO315_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5889900.1"
                     /translation="KVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPND
                     SNSVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQ
                     VTYP"
     gene            435..1229
                     /gene="panB"
                     /locus_tag="OO315_000890"
     CDS             435..1229
                     /gene="panB"
                     /locus_tag="OO315_000890"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5889901.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1241..2092
                     /gene="panC"
                     /locus_tag="OO315_000891"
     CDS             1241..2092
                     /gene="panC"
                     /locus_tag="OO315_000891"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5889902.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OO315_000892"
     CDS             complement(2166..3068)
                     /gene="rpnC"
                     /locus_tag="OO315_000892"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5889903.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            3342..3722
                     /gene="panD"
                     /locus_tag="OO315_000893"
     CDS             3342..3722
                     /gene="panD"
                     /locus_tag="OO315_000893"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5889904.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3726..4955)
                     /locus_tag="OO315_000894"
     CDS             complement(3726..4955)
                     /locus_tag="OO315_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5889905.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(5019..5459)
                     /locus_tag="OO315_000895"
     CDS             complement(5019..5459)
                     /locus_tag="OO315_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5889906.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5564..6334)
                     /locus_tag="OO315_000896"
     CDS             complement(5564..6334)
                     /locus_tag="OO315_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5889907.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6331..7257)
                     /locus_tag="OO315_000897"
     CDS             complement(6331..7257)
                     /locus_tag="OO315_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5889908.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7366..8028
                     /gene="can"
                     /locus_tag="OO315_000898"
     CDS             7366..8028
                     /gene="can"
                     /locus_tag="OO315_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5889909.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OO315_000899"
     CDS             complement(8069..8605)
                     /gene="hpt"
                     /locus_tag="OO315_000899"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5889910.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8811..11201
                     /gene="gcd"
                     /locus_tag="OO315_000900"
     CDS             8811..11201
                     /gene="gcd"
                     /locus_tag="OO315_000900"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5889911.1"
                     /translation="MAINNTASRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQIHADNVHKLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPIIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPTKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPSNLGMFEWGGISVDPNREVAIANPMALPFVSKLLPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OO315_000901"
     CDS             complement(11248..12798)
                     /gene="cueO"
                     /locus_tag="OO315_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5889912.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12964..13311
                     /locus_tag="OO315_000902"
     CDS             12964..13311
                     /locus_tag="OO315_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5889913.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13417..14283
                     /gene="speE"
                     /locus_tag="OO315_000903"
     CDS             13417..14283
                     /gene="speE"
                     /locus_tag="OO315_000903"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5889914.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14299..15093
                     /gene="speD"
                     /locus_tag="OO315_000904"
     CDS             14299..15093
                     /gene="speD"
                     /locus_tag="OO315_000904"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5889915.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKARYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15131..15493)
                     /locus_tag="OO315_000905"
     CDS             complement(15131..15493)
                     /locus_tag="OO315_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5889916.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OO315_000906"
     CDS             complement(15668..18265)
                     /gene="acnB"
                     /locus_tag="OO315_000906"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5889917.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVNVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18620..20473
                     /locus_tag="OO315_000907"
                     /pseudo
     CDS             18620..20473
                     /locus_tag="OO315_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OO315_000908"
     CDS             complement(20544..21968)
                     /gene="lpdA"
                     /locus_tag="OO315_000908"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5889918.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OO315_000909"
     CDS             complement(22293..24173)
                     /gene="aceF"
                     /locus_tag="OO315_000909"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5889919.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OO315_000910"
     CDS             complement(24188..26851)
                     /gene="aceE"
                     /locus_tag="OO315_000910"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5889920.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OO315_000911"
     CDS             complement(27012..27776)
                     /gene="pdhR"
                     /locus_tag="OO315_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5889921.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28317..29690
                     /gene="aroP"
                     /locus_tag="OO315_000912"
     CDS             28317..29690
                     /gene="aroP"
                     /locus_tag="OO315_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5889922.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OO315_000913"
     CDS             complement(29733..30587)
                     /gene="ampE"
                     /locus_tag="OO315_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5889923.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OO315_000914"
     CDS             complement(30584..31135)
                     /gene="ampD"
                     /locus_tag="OO315_000914"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5889924.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31223..32116
                     /gene="nadC"
                     /locus_tag="OO315_000915"
     CDS             31223..32116
                     /gene="nadC"
                     /locus_tag="OO315_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5889925.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32319..32759
                     /gene="ppdD"
                     /locus_tag="OO315_000916"
     CDS             32319..32759
                     /gene="ppdD"
                     /locus_tag="OO315_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5889926.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32769..33916
                     /gene="tadA"
                     /locus_tag="OO315_000917"
                     /pseudo
     CDS             32769..33916
                     /gene="tadA"
                     /locus_tag="OO315_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33906..35108
                     /gene="hofC"
                     /locus_tag="OO315_000918"
     CDS             33906..35108
                     /gene="hofC"
                     /locus_tag="OO315_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5889927.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OO315_000919"
     CDS             complement(35143..36186)
                     /gene="guaC"
                     /locus_tag="OO315_000919"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5889928.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(36348..36386)
                     /locus_tag="OO315_000920"
     CDS             complement(36348..36386)
                     /locus_tag="OO315_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889929.1"
                     /translation="MIIKTLKMSARK"
     gene            36411..37031
                     /gene="coaE"
                     /locus_tag="OO315_000921"
     CDS             36411..37031
                     /gene="coaE"
                     /locus_tag="OO315_000921"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5889930.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37031..37774
                     /gene="zapD"
                     /locus_tag="OO315_000922"
     CDS             37031..37774
                     /gene="zapD"
                     /locus_tag="OO315_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5889931.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37784..37981
                     /gene="yacG"
                     /locus_tag="OO315_000923"
     CDS             37784..37981
                     /gene="yacG"
                     /locus_tag="OO315_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5889932.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OO315_000924"
     CDS             complement(38081..38470)
                     /gene="mutT"
                     /locus_tag="OO315_000924"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5889933.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OO315_000925"
     CDS             complement(38530..41235)
                     /gene="secA"
                     /locus_tag="OO315_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5889934.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OO315_000926"
     CDS             complement(41297..41809)
                     /gene="secM"
                     /locus_tag="OO315_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5889935.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OO315_000927"
     CDS             complement(42040..42957)
                     /gene="lpxC"
                     /locus_tag="OO315_000927"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5889936.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OO315_000928"
     CDS             complement(43058..44209)
                     /gene="ftsZ"
                     /locus_tag="OO315_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5889937.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OO315_000929"
     CDS             complement(44270..45532)
                     /gene="ftsA"
                     /locus_tag="OO315_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5889938.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OO315_000930"
     CDS             complement(45529..46359)
                     /gene="ftsQ"
                     /locus_tag="OO315_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5889939.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OO315_000931"
     CDS             complement(46361..47281)
                     /gene="ddlB"
                     /locus_tag="OO315_000931"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5889940.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OO315_000932"
     CDS             complement(47274..48749)
                     /gene="murC"
                     /locus_tag="OO315_000932"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5889941.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OO315_000933"
     CDS             complement(48803..49870)
                     /gene="murG"
                     /locus_tag="OO315_000933"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5889942.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OO315_000934"
     CDS             complement(49867..51111)
                     /gene="ftsW"
                     /locus_tag="OO315_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5889943.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OO315_000935"
     CDS             complement(51111..52427)
                     /gene="murD"
                     /locus_tag="OO315_000935"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5889944.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OO315_000936"
     CDS             complement(52430..53512)
                     /gene="mraY"
                     /locus_tag="OO315_000936"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5889945.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OO315_000937"
     CDS             complement(53506..54864)
                     /gene="murF"
                     /locus_tag="OO315_000937"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5889946.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OO315_000938"
     CDS             complement(54861..56348)
                     /gene="murE"
                     /locus_tag="OO315_000938"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5889947.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OO315_000939"
     CDS             complement(56335..58101)
                     /gene="ftsI"
                     /locus_tag="OO315_000939"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5889948.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OO315_000940"
     CDS             complement(58117..58482)
                     /gene="ftsL"
                     /locus_tag="OO315_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5889949.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OO315_000941"
     CDS             complement(58479..59420)
                     /gene="rsmH"
                     /locus_tag="OO315_000941"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5889950.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OO315_000942"
     CDS             complement(59422..59880)
                     /gene="mraZ"
                     /locus_tag="OO315_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5889951.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14473 a        16732 c        14850 g        14364 t
ORIGIN      
        1 aaaggttggt actgaaaacg ggcaactcct tggtaatact ctcacaggta atgatgcagc
       61 aaaaggagtc ggtgtactca tagaaggttt agcaactagt aaaaatcctc taatgacatt
      121 gaaacctaat gattcaaatt ctgtttataa agattacgac ccgagaggca aagacgatac
      181 aacaggagga gtttacccgg atcaagatac aggtataaca taccctctcc atttccaggc
      241 cacgctacaa caggatggaa ctataccaat agaagctggt gagtttaaag ccaccagtac
      301 tttccaggta acctaccctt aataagtcca ccgcaccgcc atcatctggc ggtgcggtaa
      361 ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca cgacatcatt
      421 tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat aagcaggata
      481 aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta tttgctgatg
      541 aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag ggccacgatt
      601 ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta cgtcgcggcg
      661 caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg ccggaacaag
      721 cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa attgagggcg
      781 gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct gtatgtggtc
      841 acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta caggggcgcg
      901 gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc gcgggggcac
      961 agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc gaagcactgg
     1021 cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc ctcgtgatgc
     1081 acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat ttcctcgccg
     1141 aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag tccggcgttt
     1201 atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta tcgaaaccct
     1261 gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg tggcgctggt
     1321 gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag ccaaagcccg
     1381 cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc gcccggaaga
     1441 tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca aacgtaaagt
     1501 ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg aaacccacac
     1561 ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc cgggacattt
     1621 tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg acatcgcctg
     1681 cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg ccgatatggg
     1741 cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc tggcgctaag
     1801 ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc tgtacaaagt
     1861 tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg aaattattac
     1921 cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc agattcgcga
     1981 tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc tggtagccgc
     2041 ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt aatacttaac
     2101 tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt cataacacag
     2161 gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt tgccatctct
     2221 gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc ttgcctttcc
     2281 tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca ctgcgccagc
     2341 gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa caaatccgct
     2401 tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg acttccgcta
     2461 gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa gtcgcgctgg
     2521 cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat gatttcgtca
     2581 tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg tcgcgccagc
     2641 tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt ggcctcgccc
     2701 tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc cagatgccgg
     2761 tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc cggcttgctt
     2821 tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat ggaatagagc
     2881 acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc taaatgaagc
     2941 gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag aaagtcgcga
     3001 gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt ggtactcggt
     3061 gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca gtcaaggcgt
     3121 gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat atcaattcgt
     3181 tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca aatgccatca
     3241 gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct caaagcaggc
     3301 accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc acgatgctgc
     3361 agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa ggttcttgcg
     3421 ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc atcgatatct
     3481 ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa cgcggttcga
     3541 gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat attgtcatca
     3601 tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc aatgtcgcct
     3661 attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta caggttgctt
     3721 gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag cgaatccgtt
     3781 cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac tttgcctttc
     3841 atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc tttgtcatta
     3901 aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc cagagcgcgg
     3961 cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa tatgggtcgg
     4021 cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa aacatcgcga
     4081 atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca cttctgcggg
     4141 tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata ttgtttcaac
     4201 acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat caccaccgct
     4261 cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat gctcagtgtc
     4321 gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac cgaggtggtc
     4381 gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg ataagttagc
     4441 accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag tcgatcgcca
     4501 atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa aatcgggtag
     4561 cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc gttgtagacc
     4621 ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag gtcacccaaa
     4681 ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc gataaaccct
     4741 ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac ggcaatgatt
     4801 tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc ccagaccgca
     4861 gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact tgcggtaaac
     4921 agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag ggtgaaacaa
     4981 cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata caaattatgt
     5041 ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac ctccggccca
     5101 cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg gcaagccatc
     5161 atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga gtgtttgtgg
     5221 cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa gaagatgaca
     5281 ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag catattagag
     5341 cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg ttttttctcc
     5401 agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca acctaacatc
     5461 gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag ggtgatagcc
     5521 gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta cgcaaaccac
     5581 gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag accaccagta
     5641 cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag cggaaaccac
     5701 tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc ggcaacaaag
     5761 tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag gttggcacca
     5821 ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca agggagaata
     5881 gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc tgaaacggca
     5941 caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc gccacgccac
     6001 cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc agctcttcaa
     6061 tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag gcgttggtga
     6121 tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag ccatgcatat
     6181 cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc atggtgatga
     6241 ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct ttcgcccaga
     6301 tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt tttcattaac
     6361 cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta atacctgaat
     6421 gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta gcacttcaac
     6481 ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg gtaacggact
     6541 tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca gcgccttcat
     6601 cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc acagcatttc
     6661 tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt ttaaatcctt
     6721 caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg gttcgtcgag
     6781 aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc gcttcatccc
     6841 gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct gcttaagata
     6901 tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg cctgattcac
     6961 cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca ccagtcccaa
     7021 ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc tgacccgccc
     7081 ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc cggccccgtt
     7141 cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc cacgaagcgc
     7201 ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa tggtcataaa
     7261 tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc ggagttgccc
     7321 tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa agacatagat
     7381 acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga tcccgggttt
     7441 tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg ttccgacagt
     7501 cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt tcaccgtaat
     7561 gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca gtatgcagtg
     7621 gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg cggcgtacaa
     7681 gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca tatccgcgat
     7741 atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg tctggatacc
     7801 ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac cattatgcaa
     7861 tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg cattcacgac
     7921 ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga gcaacgttac
     7981 cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa atgccatgcc
     8041 ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact ttgccaatat
     8101 acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca aactcatccg
     8161 ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac ggtttatcca
     8221 gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca cgcactttcg
     8281 acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg ccacggatat
     8341 cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg ctaccgtagc
     8401 tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac aggtccgcca
     8461 taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg ctgtctttgt
     8521 aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc gcttcgggga
     8581 tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac aaatcacttc
     8641 gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc agtaaaacta
     8701 ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac atgttaaaac
     8761 ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt atggcaatta
     8821 acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca gcgctttgcg
     8881 ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc tggtactacc
     8941 ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt aaacgcgccg
     9001 cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc tgggaagttg
     9061 gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc ggcatctggc
     9121 tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca gttgccgcac
     9181 tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt aacgatccgc
     9241 aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc tcccccgttg
     9301 ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt tcaccactga
     9361 agcaaattca cgccgataac gtccacaagc tgaaagaagc ctgggtgttc cgtactggcg
     9421 atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg attaaagtgg
     9481 gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat gccgccagcg
     9541 gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc cagcacgtaa
     9601 cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg gaagtgatgg
     9661 cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt gcgattaacg
     9721 ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat ctgcaaagca
     9781 atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt atcaccgata
     9841 aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa acgtctggcg
     9901 tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat ccgggcgcga
     9961 aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca ccaaactcgt
    10021 gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg ggcgtgacca
    10081 cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc agctcgattc
    10141 tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt caccacgacc
    10201 tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt aatggtcaga
    10261 aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc gatcgtcgta
    10321 atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct gccaaaggcg
    10381 attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg aaagatttga
    10441 gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc gtgatgttcc
    10501 accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg ctggtcttcc
    10561 cgagtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat cgtgaagtgg
    10621 caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt ggtcctggca
    10681 acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc attcagccac
    10741 agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt ggtctgccat
    10801 gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat gaagtggtgt
    10861 ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg gttccggtgc
    10921 cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt aacgtgctgt
    10981 ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac ggtgaaaaac
    11041 tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat gaagtgaatg
    11101 gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg aagatgggcg
    11161 actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc ccggagagat
    11221 ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca tccccgtatc
    11281 ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg cgttgtgatt
    11341 aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt tccagcccgc
    11401 gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg tgccgtgaat
    11461 atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt cgtactgccc
    11521 tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga ttttattggc
    11581 atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt gccccatatg
    11641 gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt tctccattag
    11701 catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct tgcgtaccgt
    11761 cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca aagcaccgga
    11821 ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa acggtgcaat
    11881 cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt tgtcgttaac
    11941 ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca ccttcaccgg
    12001 ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt cgctggtggc
    12061 gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc aaccacgcgg
    12121 ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa accagcccac
    12181 ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa atttcttatc
    12241 ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta atttcaggat
    12301 ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc gcccggtctt
    12361 gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt tcaacgtcac
    12421 cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt cacccggtac
    12481 ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga tatcaaccgt
    12541 taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt tatagcccca
    12601 ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta actgaatgcg
    12661 attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt ccgccgcaaa
    12721 gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg aatatttcaa
    12781 gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca aaatcaaacg
    12841 ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt aatcgacaat
    12901 aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc cctggtagaa
    12961 gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt ttgcgcaaac
    13021 agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc agtttttgtc
    13081 tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg tcgcgcactg
    13141 gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt cgacatgaaa
    13201 gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt cgctaaaaaa
    13261 tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata atccctgata
    13321 cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta tgttgcgccc
    13381 tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa acagtggcat
    13441 gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct gtatcatgaa
    13501 aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg cgtaatggcg
    13561 ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga gatgatgacc
    13621 catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg cggcggcgac
    13681 ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac gatggtggaa
    13741 atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa cgccggtagc
    13801 tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt taatcaaacc
    13861 agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc cggcgaaagc
    13921 ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg cggtatcttc
    13981 gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag ccatcgcaaa
    14041 ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac ctattacggc
    14101 ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct ctcaaccgaa
    14161 attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa tccggcaatc
    14221 catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc acagccgtcc
    14281 taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa tctgaccaaa
    14341 agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga gcgcgacggt
    14401 tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat cctgtcagaa
    14461 acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta cgaaccacag
    14521 ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact catcgacaaa
    14581 acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa aagtcatatt
    14641 tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt ccgcgccgat
    14701 attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta cctgatccac
    14761 cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac tcgcgacatt
    14821 aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt tatgtctgac
    14881 gatatgaagg cgcggtatga catggtggat gtaaacgtct atcaggaaaa tatcttccat
    14941 accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac caaaccggaa
    15001 gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga aatgcgcgag
    15061 atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc ccaggctccg
    15121 ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc tgcagaaaat
    15181 cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc tcttccattt
    15241 catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg tccatccaca
    15301 gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt gcgtgcgctg
    15361 cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta aaccagtgcc
    15421 cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata tcgcgcagaa
    15481 attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg catcgcgcca
    15541 ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata gtttagaaat
    15601 aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca gcgcgtgcgc
    15661 ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg tactgagaaa
    15721 gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact tgcgcaacgt
    15781 aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca gccgccagtt
    15841 ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag ttacgggtag
    15901 aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata cacagggaac
    15961 agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag ccttcttccg
    16021 tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc ggcaactgac
    16081 ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta gtcatgcaag
    16141 aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga cgtgcgtcat
    16201 ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg atgtcgatca
    16261 ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc agccattttt
    16321 ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc gcgatcatcc
    16381 acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg ttcagcttga
    16441 tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag gcctgctcaa
    16501 ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg tttttcttgc
    16561 ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg gcgtgtacca
    16621 gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga acggattccg
    16681 gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca gaacccgccg
    16741 ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg acggtatccg
    16801 gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc agcgacacac
    16861 cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca actggcttcg
    16921 gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag cccaggcacg
    16981 ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag cctacagaag
    17041 tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag gcacggccta
    17101 ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc gcctgacgga
    17161 acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg ccacgcccga
    17221 taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg gttttcagtt
    17281 cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg tacgggtaaa
    17341 cgtcaatcac gtcgcccatg ttcaggttag agacgttgac ttcgattggc agtgcacccg
    17401 cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac aaaccaccgc
    17461 cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg gagttcgtgg
    17521 cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc gggaaacctt
    17581 tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg tctggctcaa
    17641 taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc gggcgtgacc
    17701 acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact ttaaagacgg
    17761 taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca tccgcccagg
    17821 actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct acgtcataga
    17881 agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt gccagtttgg
    17941 catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg gtgcccagca
    18001 gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta gcgatagcag
    18061 ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca cggttggtta
    18121 acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc tctacaagcg
    18181 cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca cgctcagcta
    18241 cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc atagtgcggc
    18301 aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc ccagtaccgc
    18361 aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa agcaacataa
    18421 aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa aaacttcgtt
    18481 aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa aatttaaact
    18541 accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg accattgcga
    18601 tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg tgctggcgct
    18661 aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa gccgcacagt
    18721 ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg ccgctgttac
    18781 gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat ccgcattcag
    18841 cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg ccctactttc
    18901 gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc aatggtcgca
    18961 cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc agccgtggga
    19021 cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg gcgaaaaccc
    19081 gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg tgatggactc
    19141 ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat caaccgaaca
    19201 gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca cggcacccgt
    19261 cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg caacaaccgt
    19321 catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca acccaaccgt
    19381 ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac caccagccgg
    19441 agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg ttgctaccac
    19501 gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc atcatcatga
    19561 cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct ggcaacacaa
    19621 cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg agcatcttac
    19681 tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc cctatcatga
    19741 tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga gtgccacgca
    19801 gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt ttcagcaacg
    19861 aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg cacagcggtt
    19921 taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc gtcgccaacc
    19981 actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct ctcctgagca
    20041 gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc gaagggaggc
    20101 ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc gcgagaaaat
    20161 gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta ttcagtctgc
    20221 aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac tgaaccaaca
    20281 gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac gtcaggtttt
    20341 tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc atactgtcag
    20401 gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg gagcaagaag
    20461 actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt ttttaattgc
    20521 cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg caggtcggta
    20581 atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt cgggtgcgcg
    20641 tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag gccgatttca
    20701 cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg gtgagattct
    20761 ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc acgaccagaa
    20821 gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc ttctttctca
    20881 gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat aactttcgga
    20941 tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc gtgaacacct
    21001 ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg cggtacgttg
    21061 gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac gccagctttg
    21121 cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac ggcgtcgtaa
    21181 cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc gtcttctttc
    21241 gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct gatacgcttg
    21301 gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa catttcaacc
    21361 acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc gatgataccg
    21421 ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc agtggagtcc
    21481 cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga acccgctgca
    21541 atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac ttccagggtg
    21601 ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc tttcgccata
    21661 ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg aatcttgtca
    21721 atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt ggcttcttcg
    21781 ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt caggcaaaca
    21841 ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc agcgcaacgg
    21901 aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt tttgatttca
    21961 gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt ctttcaggcc
    22021 gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc tcctggggat
    22081 tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag taatacccta
    22141 accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa tttttaaaca
    22201 acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg agattaccag
    22261 aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg aatgtcagac
    22321 agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat cacgcggtgg
    22381 tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc attccacacc
    22441 ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt cacaatcggc
    22501 gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc gccctgcatt
    22561 tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag ctcgcgagac
    22621 agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag accgttcggg
    22681 gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg accgtcttcc
    22741 gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc tttcatgatg
    22801 aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc gttctgctgt
    22861 ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac atgcgggatc
    22921 atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc cagttccact
    22981 tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat gccagggata
    23041 ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt cacgtaagcc
    23101 tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt cgccaggtta
    23161 acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta agcgtcgttt
    23221 tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt tgctgccgga
    23281 gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc ttcaacttcg
    23341 aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt cagttccttc
    23401 acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtaatc
    23461 agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt cacttcaact
    23521 tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg tgcaggggtc
    23581 gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc acccgcgact
    23641 tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac tttgatctct
    23701 ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc ttctacggtg
    23761 atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc ggtcacttca
    23821 acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc cgcgggtgct
    23881 gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc tgctgcaccg
    23941 tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc aacagagact
    24001 ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc tttgtcgcct
    24061 tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac caggatctcg
    24121 gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc cattattctt
    24181 ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat ttggcgattg
    24241 cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc agcgccgcaa
    24301 ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg ctgtcggaac
    24361 gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa gtacggacct
    24421 gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg ttcatcacct
    24481 gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag cgttcacaat
    24541 cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca gaacctacgc
    24601 cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg gaaccggagc
    24661 ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag atacctttac
    24721 ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg ttcagcgtag
    24781 tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga ccgtcatgca
    24841 taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc gggatagtca
    24901 gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc agggtggtac
    24961 gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc gcccagcaca
    25021 gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg atcatcggca
    25081 gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg cccagctcgt
    25141 tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca acctgctcgc
    25201 ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga cggaacagac
    25261 cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga tctttgatcg
    25321 acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta gagagctctt
    25381 tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca agcttctcgg
    25441 tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga gcgtgcagat
    25501 aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg atatctgcat
    25561 cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg ttcattttct
    25621 taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa cctttaatgg
    25681 tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg aatgcagcgt
    25741 agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg tcagtccagt
    25801 ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga acgtacgcac
    25861 catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc agctggatca
    25921 gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac atcactttga
    25981 tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc ttgccgttac
    26041 cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc aggttatcca
    26101 gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc atttcaccgt
    26161 cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg tgttccagat
    26221 atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata gatacggtcg
    26281 ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg ccattgccgt
    26341 gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc aggaaagcac
    26401 gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg ccatcctgct
    26461 cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg gaagactgga
    26521 aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc accgtcatga
    26581 tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta cccggatact
    26641 ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg cctgcggcta
    26701 cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac tgagcacgct
    26761 caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga gtttcgatcg
    26821 gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc taataacgtt
    26881 gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag acaggttcta
    26941 cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga taatttttac
    27001 cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc acggcggctc
    27061 tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg atgcgatgct
    27121 tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt gcggtgacta
    27181 ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg gcggacattc
    27241 tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt gtgggccgct
    27301 tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag atcgccagac
    27361 tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc ttcatcggta
    27421 ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt ttcgagcaag
    27481 tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct gaagctttgc
    27541 cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa gcccttcgct
    27601 tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg ttttgccagt
    27661 tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat caaaaactcc
    27721 agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta ggccatgagt
    27781 tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt ctgtgacgct
    27841 aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca tgaaactgtg
    27901 cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca caaatgtaga
    27961 ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg aattgaaccg
    28021 attcacttac caattttttg atttttaagt caataaaaac gaatttaaat tcactctgca
    28081 tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa ctcatcttca
    28141 accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc tgacggaagt
    28201 ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa acggaattgc
    28261 aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga ggtttcatga
    28321 tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc catattcagc
    28381 ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc tccgtaatac
    28441 agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc gcctttctga
    28501 tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc tttagccact
    28561 ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac tactgggtac
    28621 tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt cagttctggt
    28681 atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt aacgccatca
    28741 acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt atcaaagtta
    28801 ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc aacggcggcc
    28861 cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac ggcttcaccg
    28921 gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa ctggtgggga
    28981 tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact aaccaggtta
    29041 tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca ctgatgccgt
    29101 ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag ttaggcgata
    29161 cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc gtgtacaaca
    29221 gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg aacgcgccaa
    29281 aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg gtgtctgcgc
    29341 tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca tttggcctgt
    29401 taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt ctggcgcata
    29461 tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct gctctgcttt
    29521 atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg attatgctga
    29581 tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc gtgttaggta
    29641 tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa tctctctacg
    29701 ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta aatcgtcagt
    29761 agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac cgctgccttc
    29821 ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc cagacgcgtt
    29881 aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc cggtaacgct
    29941 ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg cacccaatcc
    30001 agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt ctggtatcgc
    30061 gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac gggtccccag
    30121 gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt aatccacagc
    30181 aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg gacgccgtga
    30241 atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg tgaagcagct
    30301 gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag caaaccaatc
    30361 agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc gcgtaacagt
    30421 aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag agaaaaatgt
    30481 ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc gcccaactta
    30541 aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt gtctccttgc
    30601 tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt ttccgatccg
    30661 gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga tctatcagtg
    30721 cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga gtatcggtgc
    30781 cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga tactgagaga
    30841 ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca ccatcacggc
    30901 gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa ggatgtgcct
    30961 gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac tcgcctggcg
    31021 gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg cggcaatcgt
    31081 aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac aacatgcagg
    31141 atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac cttatgattc
    31201 gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca cccgacgtga
    31261 cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg cgctgcggga
    31321 agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt taccggaaaa
    31381 ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg gcaaacgctg
    31441 ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct ggcatgtgga
    31501 tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc catcccgcgt
    31561 gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag gagttgccag
    31621 taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt tggatacgcg
    31681 caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg gcggcggagc
    31741 gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata ttattgcctc
    31801 cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg cgccagtaga
    31861 agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag ccgatatcat
    31921 catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca ccaacggcaa
    31981 ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat ttgccgaaac
    32041 gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac tcgacctttc
    32101 aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg ataatctccc
    32161 cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc aacgaaataa
    32221 aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc tgatggcgtt
    32281 tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa cgcggtttta
    32341 cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc attggtattc
    32401 ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa acctttgtgc
    32461 cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat acctgcgacg
    32521 gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc atgagtgtgg
    32581 caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc gtcgtcatga
    32641 cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc aatattcaaa
    32701 gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac gccaactaag
    32761 gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag ggagtcttgc
    32821 tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca catgagttgc
    32881 tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg acgcgccaac
    32941 aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt caggagaagc
    33001 atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa caacgcgcgt
    33061 ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt atcgacggcg
    33121 tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca gccagattaa
    33181 aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg caattcactg
    33241 tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt cggggtggtg
    33301 aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt aacacgctgg
    33361 gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca cagggactgg
    33421 tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt ccacgctaca
    33481 caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg cccgctggat
    33541 gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac tttgcccaca
    33601 ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc cgtcgccgct
    33661 gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg gtcgtaccgc
    33721 cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg ctaatactga
    33781 agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt ttgaaaacgg
    33841 ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg tactggggat
    33901 gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac ggcaatgcgc
    33961 aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta cagcaacaga
    34021 tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg cgaggagata
    34081 aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta acgctttctg
    34141 aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg ttgctgcaat
    34201 cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta ccctggtcag
    34261 aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc ggtaagctgg
    34321 atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg accgacaaag
    34381 tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg gtggttgtgg
    34441 caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc aacaccccac
    34501 taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa tggggctggc
    34561 tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg cgccgcccga
    34621 cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt tcactgatga
    34681 ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt gcaggcatta
    34741 cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac tgggtgcaac
    34801 ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg gcgctaaaaa
    34861 atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag gcatccggct
    34921 cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca atggcgctgg
    34981 cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga ggaattatcg
    35041 gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg atgagtggga
    35101 tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt tgttgaagat
    35161 gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct ctttcaggcg
    35221 tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac gcgcggtatt
    35281 ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc ggtattccgc
    35341 aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga acagcataaa
    35401 tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg ccagcatgcc
    35461 gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc ccggcgtggt
    35521 acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg cacattcgat
    35581 taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac aaacagaacc
    35641 tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct cacacatttc
    35701 accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac gcgctttcgc
    35761 aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa cgaagtttaa
    35821 tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag acgtgccggt
    35881 agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg cttgccactc
    35941 ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca gcgcagaggc
    36001 catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc cggaccagct
    36061 ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac ggcttttaag
    36121 agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat cttcttcaat
    36181 acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac tccagtgacg
    36241 ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt ttacgctaca
    36301 atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca ctttcgcgcg
    36361 ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt atggggtata
    36421 tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat gcgtttgctg
    36481 atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt gaaccaggtg
    36541 cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct gctgatggaa
    36601 cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag aaaaactggc
    36661 ttaacgacct gctgcatccg ctgattcagc aagagacgca acaccagatc cagcaagcaa
    36721 cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg tataaaaaag
    36781 cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc accatgcagc
    36841 gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca acgcgcgaag
    36901 cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat gctatcgcat
    36961 cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt gtctcacagg
    37021 aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa aaatgcgtac
    37081 atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca tcgttgacca
    37141 cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt tcgagcgcgg
    37201 cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac tccagacctg
    37261 gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc agttaaaagc
    37321 ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg aagatcgttt
    37381 gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct ttgatttacc
    37441 tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg tagaaacctg
    37501 gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt taattcgcca
    37561 gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata acggtggcga
    37621 tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc aaatttccgg
    37681 acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg gacaggtacc
    37741 ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag aaactattac
    37801 ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc cgtttcggcc
    37861 attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg aagaaaaacg
    37921 aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc caaagcagtg
    37981 acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc atcaggcatc
    38041 ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc ttaagcttcg
    38101 caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc agcgacatcc
    38161 actcaccggg ttgcccttct ttaccccacg gcaccccttc ccaactttcg accagccaga
    38221 accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat agcgaaaaat
    38281 gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt tccggcgttt
    38341 cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc gcatctgctg
    38401 cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc gcaatttgca
    38461 gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt ttacttcaac
    38521 tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag aaccgcacgg
    38581 gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg cagctgcggc
    38641 tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct cggcttccat
    38701 acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa ctttgctcag
    38761 cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg agaacgattc
    38821 acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga taccctgacg
    38881 cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt gcagcatgac
    38941 gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac gctgatacac
    39001 ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt ctggttcttt
    39061 atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac gttcctgcag
    39121 ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat caatggtggc
    39181 tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga catccagcag
    39241 ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat catattccag
    39301 cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt tggcaatcgc
    39361 tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt tacgcatcat
    39421 gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg acaggtagaa
    39481 acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt tatcgatacg
    39541 acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca gtaccgcatc
    39601 gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg gattttccag
    39661 cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac gacccgccat
    39721 gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg ccgcttcgtt
    39781 ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca gttcgtttga
    39841 caccagctcc gatttttcga tggagatagt acccaccagc accggctggc ctttcgcagt
    39901 acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca tgtagaccag
    39961 gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg tatccagttt
    40021 gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc ccgccagttt
    40081 ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt tttcgttctg
    40141 gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc agcgtcggcc
    40201 ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt taacgatgta
    40261 gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt ggtgcatcag
    40321 catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct ctttcaccag
    40381 cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag atttttcgtc
    40441 caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct gacggatcag
    40501 gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg ccgggccgga
    40561 aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt ccaccagcgc
    40621 atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt cgcgcaggta
    40681 gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag cttcgcgctt
    40741 tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga attcaaacag
    40801 cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa ctacgtgcac
    40861 gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg ttttaccttc
    40921 accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga gtaactgaac
    40981 gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg cgaaagcttc
    41041 cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact ctgcggtttt
    41101 ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat tgatgatgtt
    41161 gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa ctttagttaa
    41221 caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa taaaatgaag
    41281 taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct tgcgcctggc
    41341 ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa tcaatgcgat
    41401 aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta tccagtaatg
    41461 caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt tgcggtgcca
    41521 ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag taatcaacgg
    41581 aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga ccaaagttaa
    41641 ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt ggttcggcgg
    41701 cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag agatgcggcc
    41761 agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg ttattgccat
    41821 cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct gaagtccaca
    41881 gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc gcaccgtttt
    41941 ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga gagagtgcca
    42001 gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta cagctgaagg
    42061 cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt cccaggcttc
    42121 ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg atttataagc
    42181 ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg catcgagcat
    42241 tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta cgcgataatc
    42301 gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg actgcagata
    42361 ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca taaacgcatc
    42421 agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat ggttaaaatc
    42481 gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact tatcgccatc
    42541 ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca actcgtcgat
    42601 accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga tcgggatttc
    42661 cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag cagcattgag
    42721 gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac agagcatggt
    42781 atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt cggtgcgacg
    42841 atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt tcttgccggt
    42901 atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt tgatcatcgt
    42961 attatctcgc caaattacct atccaaccga agtgtactat acattcggcg ggccagttta
    43021 gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc ttacgtagga
    43081 atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg tcattcacta
    43141 ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc tggtagcgat
    43201 ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga cgtttgtcca
    43261 tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc gggtcaagag
    43321 aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg ttacctaccg
    43381 tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc acgccgcgcg
    43441 cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca gcagcttctt
    43501 ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc atttcagaca
    43561 ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta atcagttcgg
    43621 cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca tccagcaggg
    43681 agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc agagagtcca
    43741 catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc ttgccttcaa
    43801 agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt gccacttcag
    43861 cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca ataaagacca
    43921 tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc gcattgcggc
    43981 caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg atttgaatcg
    44041 tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg aagaattcaa
    44101 caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg ccgccgacgc
    44161 cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata gtttctctcc
    44221 gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat taaaactctt
    44281 ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta acacgttttt
    44341 ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat cccaccgccg
    44401 tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc ggcgcgccga
    44461 tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg atctgcgctg
    44521 cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt tggcgaagct
    44581 tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta tagcgcggct
    44641 cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg cctacgctcg
    44701 gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg tggcgaactt
    44761 taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca ctggtcacga
    44821 cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta taaacggcga
    44881 tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt tcatcttccg
    44941 tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact ttcagcccac
    45001 aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg atcaggtgca
    45061 cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct tcctgatagt
    45121 caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc acacgcaccg
    45181 atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct tcagaaatag
    45241 gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc agatataccg
    45301 aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt acgcacttga
    45361 ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg cagctgccca
    45421 cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg gcaaccttcg
    45481 cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc attgttgttc
    45541 tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg cccagcctac
    45601 tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat cggtttgcgc
    45661 ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca tcgtatcgcc
    45721 ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac gccgcgcggt
    45781 catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca tttcgcgata
    45841 gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa gcacctgctt
    45901 gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca tgtgttgatc
    45961 attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat caggccactg
    46021 ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg tctggatgat
    46081 gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg actgccggat
    46141 atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga gcggcaggcg
    46201 ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg tcgttaaaac
    46261 ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag aagaaacctc
    46321 ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc agttccagaa
    46381 ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc ggcaccaggc
    46441 tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt ccatcactgt
    46501 ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat gctttcagca
    46561 ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag aaatactgtg
    46621 tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt tgaatacgta
    46681 ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca cttagccatt
    46741 tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct tgtagagcat
    46801 tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc ttaacgataa
    46861 ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct ttttcaaact
    46921 ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc cacagaagtt
    46981 tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg gtataaggca
    47041 agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga ccgtgtagcg
    47101 cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact tctttcgggt
    47161 cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct gcgccagaat
    47221 tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt ttatcagtca
    47281 tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga acgggcaatt
    47341 tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa taccggtgcc
    47401 agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt cccacgtcca
    47461 cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc gccagccgga
    47521 tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa atcatcatac
    47581 aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt tttatccggc
    47641 cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg gccgtagtca
    47701 tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa ttcaccgagg
    47761 aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat agcctcgtcg
    47821 tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc gttatgacgg
    47881 cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag cgtaaagtgc
    47941 ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc gctgaagccg
    48001 taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg atcatcaaca
    48061 cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa ggtctgtttt
    48121 aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt ggtgacaatc
    48181 gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc ggcaatcagg
    48241 taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc gccgttaacg
    48301 aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt agttgtcgtt
    48361 ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc agccagcatt
    48421 tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc cgggttgtcg
    48481 gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc cgggcgatgg
    48541 ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt tggcgctaaa
    48601 tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc gcccataccg
    48661 gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg cacgatggaa
    48721 cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt aaattcatac
    48781 gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca tttgccactc
    48841 gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt aataaggttt
    48901 ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt ggctgctcga
    48961 taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc tggcggtctt
    49021 tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca ctcacagtta
    49081 aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc atatcatcaa
    49141 taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt tcaacggatt
    49201 gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt ttcgcagcaa
    49261 cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact accaacacac
    49321 gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc agcacatcgg
    49381 tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac gcctgcatca
    49441 ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg ttttgttcat
    49501 gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct gatacatagc
    49561 cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc gcctgacgcc
    49621 aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt ccacgcaaac
    49681 cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc gcttccatac
    49741 ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag tgcgcaaccg
    49801 ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat cgctttcctt
    49861 gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg tttcataatc
    49921 aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac caccgtaact
    49981 gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc ctacgttaac
    50041 cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg aaaaacggtg
    50101 gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga agaataccat
    50161 taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg cgaaaataaa
    50221 gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta aaccttgccc
    50281 ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc cgctgccaaa
    50341 gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg gttcggcgag
    50401 gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact gccacaattt
    50461 cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca ccgtaccaag
    50521 gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg gtttcaggaa
    50581 gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg cgatatagca
    50641 aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga gatcgatcca
    50701 acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca ggatgataga
    50761 tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac gcagcgtaat
    50821 gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga agaagaacgg
    50881 atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga taaagccaat
    50941 cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca ggctgtcggt
    51001 atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc agaccaggat
    51061 actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca tcaacctaac
    51121 tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa ctgatcaagg
    51181 ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg agccagcaag
    51241 cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag cgccgccagc
    51301 tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag gtaacgcacc
    51361 agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag cgtgccgtct
    51421 acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt tgaatcgtta
    51481 atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag accggtgaag
    51541 gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc cagcgccagc
    51601 gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt cacattcagc
    51661 actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag gtgatagtca
    51721 cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg cattgttaag
    51781 gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag ttttgctgca
    51841 cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac gttcagaatg
    51901 gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga cagttccagc
    51961 acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat attgccaccc
    52021 acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt gaccgtgctt
    52081 ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca aaacagctcg
    52141 atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg atgcgccagt
    52201 gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc attcagactg
    52261 cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt catacgcgta
    52321 tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga aagcccggtg
    52381 aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat taacgtacct
    52441 tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa cgcacaatga
    52501 cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc atgcggaaaa
    52561 tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc gtttctacca
    52621 cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg gcgataatgc
    52681 ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc gccggatagg
    52741 tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca ataaccagtt
    52801 ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg ttgccggtcg
    52861 cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt gccagaccgt
    52921 caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag taagccagca
    52981 gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc accagctgcg
    53041 ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc gcaatgaccg
    53101 acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc accactttgc
    53161 gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac acgcaccaga
    53221 cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct gtcaggatca
    53281 taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga ccgtcgttac
    53341 gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg cccatccaca
    53401 atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga taggaaaaga
    53461 cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac atgtcccatt
    53521 ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct taactaaaat
    53581 cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg cagttttatc
    53641 agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt tacccatgct
    53701 taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat ggcaggcttc
    53761 gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc ccggcatttc
    53821 agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg agtcgtcgag
    53881 cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag ctttcagatt
    53941 tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga gcgcagcggc
    54001 tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat cgacgctacc
    54061 ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat tggtggcggt
    54121 gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc caattacgct
    54181 ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt tttccggcag
    54241 gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa aaccttccag
    54301 atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac tcacagtcca
    54361 ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt attccggcgt
    54421 taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg ccgtataaag
    54481 cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg ttttgccgga
    54541 ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg cagccagttc
    54601 accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt ccagcggacg
    54661 gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat cgtgggcatc
    54721 aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc gggtatcagt
    54781 ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa tatcggtaag
    54841 ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac cgtgacgcga
    54901 tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc tttgcccgcg
    54961 accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc ttcagcacgg
    55021 ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc gttgatgatg
    55081 gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc agcaaactct
    55141 tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc acagccaaag
    55201 acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt ttccagtgca
    55261 tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt gaacacttcc
    55321 atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc cagtggatag
    55381 cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa agcgcccatc
    55441 agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt cgcgccgctg
    55501 tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg attgatatga
    55561 tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg gcccacttca
    55621 tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag ccatttcgca
    55681 gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa gttggtaaag
    55741 accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg ggaggaaact
    55801 tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg ctgaacatca
    55861 actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc gttaccaacg
    55921 gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag aagctgggtc
    55981 gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc agagggttca
    56041 tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag atagatgacc
    56101 ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc ctctgcaata
    56161 atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc cgcctgatga
    56221 cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag tgtcatctct
    56281 cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg caaattacga
    56341 tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc ccgttgtcag
    56401 cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac caaagaccgg
    56461 cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa caacaaccag
    56521 cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat atttattgat
    56581 gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga tacgatagcc
    56641 tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca tcatatgcac
    56701 cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg ggtcaacttt
    56761 ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga ctcgcgctaa
    56821 ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct ctatgtcaga
    56881 ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca aattggtcgc
    56941 ttttcccagt ccaaaacgtg agtaagtatc tactaacgct gaggacggca tcgttaacgc
    57001 cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg ttaattcgct
    57061 gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg tattgagtac
    57121 cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg gtttaaccgt
    57181 tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt tcggcgtgcc
    57241 gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt cacctgtgtt
    57301 gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg cgttgttcag
    57361 ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt tgtgcgctgc
    57421 ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt tacgcacaat
    57481 gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc cctcaatccc
    57541 ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg acggatagta
    57601 acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga tgtagtccgc
    57661 catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt tggcgttaat
    57721 gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct tccagcggtc
    57781 accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa tcgcttttac
    57841 cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc gggaggtgga
    57901 aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat ccggggagat
    57961 aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa tacagccgca
    58021 taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt ttggtttctg
    58081 cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta cgatattttc
    58141 ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt ccacccggct
    58201 atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt ctaaagcatc
    58261 tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt gcgccgtggt
    58321 taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca gcttcccaaa
    58381 tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc ttcccatcga
    58441 tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt cctctctgca
    58501 atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc gcccggcatt
    58561 aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc ttcagtcatc
    58621 ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg tttcacaata
    58681 cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc cagcacgttg
    58741 agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca aatgcgcacc
    58801 gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt tgcagcagcc
    58861 acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg ctcgacaatg
    58921 gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac ccaggcgata
    58981 tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt tgggtccata
    59041 cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc atcaagttgc
    59101 ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag atcgcgctcg
    59161 gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg cggatcatca
    59221 atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa acgcccctct
    59281 tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt cccatcaatg
    59341 tagatgccat caggacggat attgaggcca ttaacggctt catccagcag caccgtagta
    59401 tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg ataagtctcc
    59461 ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg ttgtttcatc
    59521 ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca gcccggcatg
    59581 ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac attcgctggc
    59641 atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca acagacgcga
    59701 taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc acgggtgatg
    59761 aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt cccgataacg
    59821 ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc cccggaacat
    59881 gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag gagtttacgg
    59941 agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa acccgatgtt
    60001 tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat taacgttatg
    60061 aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact cactattttc
    60121 cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca tatcttcccc
    60181 ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg cgcaaaagca
    60241 aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata atgcaaaaac
    60301 gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt aacgggcggg
    60361 tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag gatattgat
//
LOCUS       DAJTRD010000014        58101 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTRD010000014 DAJTRD010000000
VERSION     DAJTRD010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58101
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.014"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..628)
                     /gene="ycjM"
                     /locus_tag="OO315_000943"
     CDS             complement(<1..628)
                     /gene="ycjM"
                     /locus_tag="OO315_000943"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5889952.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLL"
     gene            complement(840..1187)
                     /gene="pspE"
                     /locus_tag="OO315_000944"
     CDS             complement(840..1187)
                     /gene="pspE"
                     /locus_tag="OO315_000944"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5889953.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1230..1451)
                     /gene="pspD"
                     /locus_tag="OO315_000945"
     CDS             complement(1230..1451)
                     /gene="pspD"
                     /locus_tag="OO315_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5889954.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1460..1819)
                     /gene="pspC"
                     /locus_tag="OO315_000946"
     CDS             complement(1460..1819)
                     /gene="pspC"
                     /locus_tag="OO315_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5889955.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1819..2043)
                     /gene="pspB"
                     /locus_tag="OO315_000947"
     CDS             complement(1819..2043)
                     /gene="pspB"
                     /locus_tag="OO315_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5889956.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2097..2765)
                     /gene="pspA"
                     /locus_tag="OO315_000948"
     CDS             complement(2097..2765)
                     /gene="pspA"
                     /locus_tag="OO315_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5889957.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2932..3909
                     /gene="pspF"
                     /locus_tag="OO315_000949"
     CDS             2932..3909
                     /gene="pspF"
                     /locus_tag="OO315_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5889958.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4029..5294)
                     /gene="puuE"
                     /locus_tag="OO315_000950"
     CDS             complement(4029..5294)
                     /gene="puuE"
                     /locus_tag="OO315_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5889959.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5332..6612)
                     /gene="puuB"
                     /locus_tag="OO315_000951"
     CDS             complement(5332..6612)
                     /gene="puuB"
                     /locus_tag="OO315_000951"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5889960.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(6614..8101)
                     /gene="puuC"
                     /locus_tag="OO315_000952"
     CDS             complement(6614..8101)
                     /gene="puuC"
                     /locus_tag="OO315_000952"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5889961.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8251..8808)
                     /gene="puuR"
                     /locus_tag="OO315_000953"
     CDS             complement(8251..8808)
                     /gene="puuR"
                     /locus_tag="OO315_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5889962.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8835..9599)
                     /gene="puuD"
                     /locus_tag="OO315_000954"
     CDS             complement(8835..9599)
                     /gene="puuD"
                     /locus_tag="OO315_000954"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5889963.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            9811..11229
                     /gene="puuA"
                     /locus_tag="OO315_000955"
     CDS             9811..11229
                     /gene="puuA"
                     /locus_tag="OO315_000955"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5889964.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11378..11542
                     /locus_tag="OO315_000956"
     CDS             11378..11542
                     /locus_tag="OO315_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889965.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            11532..12917
                     /gene="puuP"
                     /locus_tag="OO315_000957"
     CDS             11532..12917
                     /gene="puuP"
                     /locus_tag="OO315_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5889966.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13051..13296
                     /locus_tag="OO315_000958"
     CDS             13051..13296
                     /locus_tag="OO315_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5889967.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13609..15252
                     /gene="sapA"
                     /locus_tag="OO315_000959"
     CDS             13609..15252
                     /gene="sapA"
                     /locus_tag="OO315_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5889968.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15249..16214
                     /gene="sapB"
                     /locus_tag="OO315_000960"
     CDS             15249..16214
                     /gene="sapB"
                     /locus_tag="OO315_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5889969.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16201..17091
                     /gene="sapC"
                     /locus_tag="OO315_000961"
     CDS             16201..17091
                     /gene="sapC"
                     /locus_tag="OO315_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5889970.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17091..18083
                     /gene="sapD"
                     /locus_tag="OO315_000962"
     CDS             17091..18083
                     /gene="sapD"
                     /locus_tag="OO315_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5889971.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18085..18891
                     /gene="sapF"
                     /locus_tag="OO315_000963"
     CDS             18085..18891
                     /gene="sapF"
                     /locus_tag="OO315_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5889972.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18959..19312
                     /locus_tag="OO315_000964"
     CDS             18959..19312
                     /locus_tag="OO315_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5889973.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19680..20468
                     /gene="fabI"
                     /locus_tag="OO315_000965"
     CDS             19680..20468
                     /gene="fabI"
                     /locus_tag="OO315_000965"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5889974.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTRESFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20612..21739
                     /locus_tag="OO315_000966"
     CDS             20612..21739
                     /locus_tag="OO315_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5889975.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21807..23741
                     /gene="rnb"
                     /locus_tag="OO315_000967"
     CDS             21807..23741
                     /gene="rnb"
                     /locus_tag="OO315_000967"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5889976.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23819..23922)
                     /locus_tag="OO315_000968"
                     /pseudo
     CDS             complement(23819..23922)
                     /locus_tag="OO315_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23976..25950
                     /gene="pdeR"
                     /locus_tag="OO315_000969"
                     /pseudo
     CDS             23976..25950
                     /gene="pdeR"
                     /locus_tag="OO315_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26098..26271
                     /locus_tag="OO315_000970"
     CDS             26098..26271
                     /locus_tag="OO315_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889977.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26361..27110
                     /locus_tag="OO315_000971"
     CDS             26361..27110
                     /locus_tag="OO315_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5889978.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27379..27597
                     /gene="osmB"
                     /locus_tag="OO315_000972"
     CDS             27379..27597
                     /gene="osmB"
                     /locus_tag="OO315_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5889979.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(27723..28049)
                     /gene="yciH"
                     /locus_tag="OO315_000973"
     CDS             complement(27723..28049)
                     /gene="yciH"
                     /locus_tag="OO315_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5889980.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28049..28786)
                     /gene="pyrF"
                     /locus_tag="OO315_000974"
     CDS             complement(28049..28786)
                     /gene="pyrF"
                     /locus_tag="OO315_000974"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5889981.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(28979..30148)
                     /gene="lapB"
                     /locus_tag="OO315_000975"
     CDS             complement(28979..30148)
                     /gene="lapB"
                     /locus_tag="OO315_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5889982.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30155..30463)
                     /gene="lapA"
                     /locus_tag="OO315_000976"
     CDS             complement(30155..30463)
                     /gene="lapA"
                     /locus_tag="OO315_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5889983.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(30612..31376)
                     /gene="pgpB"
                     /locus_tag="OO315_000977"
     CDS             complement(30612..31376)
                     /gene="pgpB"
                     /locus_tag="OO315_000977"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5889984.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            31546..32136
                     /gene="ribA"
                     /locus_tag="OO315_000978"
     CDS             31546..32136
                     /gene="ribA"
                     /locus_tag="OO315_000978"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5889985.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32200..34875)
                     /gene="acnA"
                     /locus_tag="OO315_000979"
     CDS             complement(32200..34875)
                     /gene="acnA"
                     /locus_tag="OO315_000979"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5889986.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35039..35134)
                     /locus_tag="OO315_000980"
     CDS             complement(35039..35134)
                     /locus_tag="OO315_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889987.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35248..35415)
                     /locus_tag="OO315_000981"
     CDS             complement(35248..35415)
                     /locus_tag="OO315_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889988.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35418..35582)
                     /locus_tag="OO315_000982"
     CDS             complement(35418..35582)
                     /locus_tag="OO315_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5889989.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(35877..36851)
                     /gene="cysB"
                     /locus_tag="OO315_000983"
     CDS             complement(35877..36851)
                     /gene="cysB"
                     /locus_tag="OO315_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5889990.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37061..39658)
                     /gene="topA"
                     /locus_tag="OO315_000984"
     CDS             complement(37061..39658)
                     /gene="topA"
                     /locus_tag="OO315_000984"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5889991.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40038..40289
                     /locus_tag="OO315_000985"
     CDS             40038..40289
                     /locus_tag="OO315_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5889992.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40325..41374)
                     /gene="sohB"
                     /locus_tag="OO315_000986"
     CDS             complement(40325..41374)
                     /gene="sohB"
                     /locus_tag="OO315_000986"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5889993.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            41594..42352
                     /locus_tag="OO315_000987"
     CDS             41594..42352
                     /locus_tag="OO315_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5889994.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42349..42939
                     /gene="cobO"
                     /locus_tag="OO315_000988"
     CDS             42349..42939
                     /gene="cobO"
                     /locus_tag="OO315_000988"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5889995.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(42979..43851)
                     /gene="rluB"
                     /locus_tag="OO315_000989"
     CDS             complement(42979..43851)
                     /gene="rluB"
                     /locus_tag="OO315_000989"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5889996.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(43952..44572)
                     /locus_tag="OO315_000990"
     CDS             complement(43952..44572)
                     /locus_tag="OO315_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5889997.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(44569..45450)
                     /gene="yciV"
                     /locus_tag="OO315_000991"
     CDS             complement(44569..45450)
                     /gene="yciV"
                     /locus_tag="OO315_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5889998.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            45588..45632
                     /gene="trpL"
                     /locus_tag="OO315_000992"
     CDS             45588..45632
                     /gene="trpL"
                     /locus_tag="OO315_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5889999.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    45609..45702
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            45724..47286
                     /gene="trpE"
                     /locus_tag="OO315_000993"
     CDS             45724..47286
                     /gene="trpE"
                     /locus_tag="OO315_000993"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5890000.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47286..48881
                     /gene="trpD"
                     /locus_tag="OO315_000994"
     CDS             47286..48881
                     /gene="trpD"
                     /locus_tag="OO315_000994"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5890001.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            48882..50243
                     /gene="trpCF"
                     /locus_tag="OO315_000995"
     CDS             48882..50243
                     /gene="trpCF"
                     /locus_tag="OO315_000995"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5890002.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50255..51448
                     /gene="trpB"
                     /locus_tag="OO315_000996"
     CDS             50255..51448
                     /gene="trpB"
                     /locus_tag="OO315_000996"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5890003.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51448..52254
                     /gene="trpA"
                     /locus_tag="OO315_000997"
     CDS             51448..52254
                     /gene="trpA"
                     /locus_tag="OO315_000997"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5890004.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            52634..52813
                     /locus_tag="OO315_000998"
     CDS             52634..52813
                     /locus_tag="OO315_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5890005.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            52899..53399
                     /locus_tag="OO315_000999"
     CDS             52899..53399
                     /locus_tag="OO315_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5890006.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53445..53951
                     /locus_tag="OO315_001000"
     CDS             53445..53951
                     /locus_tag="OO315_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5890007.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54011..54649)
                     /gene="ompW"
                     /locus_tag="OO315_001001"
     CDS             complement(54011..54649)
                     /gene="ompW"
                     /locus_tag="OO315_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5890008.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            54889..54978
                     /locus_tag="OO315_001002"
                     /pseudo
     CDS             54889..54978
                     /locus_tag="OO315_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55006..55749
                     /locus_tag="OO315_001003"
     CDS             55006..55749
                     /locus_tag="OO315_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5890009.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            55779..56318
                     /locus_tag="OO315_001004"
     CDS             55779..56318
                     /locus_tag="OO315_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5890010.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56423..56821
                     /gene="yciA"
                     /locus_tag="OO315_001005"
     CDS             56423..56821
                     /gene="yciA"
                     /locus_tag="OO315_001005"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5890011.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(56861..57589)
                     /gene="tonB"
                     /locus_tag="OO315_001006"
     CDS             complement(56861..57589)
                     /gene="tonB"
                     /locus_tag="OO315_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5890012.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            57804..58100
                     /locus_tag="OO315_001007"
     CDS             57804..58100
                     /locus_tag="OO315_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5890013.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT        13947 a        15017 c        14533 g        14604 t
ORIGIN      
        1 ccagcaacac ttcagggcta cggtaattca ggtcaatttg atcgtcacta aaggtggtcc
       61 ataaatggcg cgtggaatta tcatccattt gaaacggcgt taatagcggt aacgcacgcg
      121 gaagggtgac ggcactgaga tcggttttcg gatcaaccgc aataaagaaa tcttcaaaac
      181 ccggaagctg ttgtaaatag tttttaaacc attcactttt tgcagacata tggttgcaga
      241 caaaatcaaa cattaaatga ctgcattcac cgagttgctg aatatcctgc cactcccccg
      301 cttcactggc gacctgatga taatcaatta ccgaaaatcc atcatcagat gaccatgggt
      361 aaaaaggtaa taaatgaaca tgtgaaaaaa tgctttgcag ccattgatgg tagaactgat
      421 taaatatggg tagtggtttt aaatcattac tgtgaaattg atcggcatag gtaattaaca
      481 cgacatcact ttcatcccag tgttttttac gtcgctgtgt aattaatcgt ttcgcactct
      541 caagacgcgt tacaagtttc tgtaaatgag ttgcggtaaa tgttccaccg tagatttcgt
      601 ccatgtaatc cgtaattttc tgtttcatgt tgcctttccg tggtagcggt cccatgaggc
      661 gagagactac tccgcagaaa ataaactgtc aattgagcac aagggtttta gtcacgcagg
      721 aaatcatttt ttaccaggga aaaagcgtat ttgtgagctg acgcaaataa tctataggga
      781 tgtaaataag gtgtgacaga aaaaaaacgg cgcataagcg ccgctcatgg tgaattcttt
      841 taacctttga ccttcggcat tgcgatgtct ttcaggccac cggcgttctc aacgtgggta
      901 tatcccatct cgctaaggag ctcttttgcc tgcccggact ggcgtccggc attgcaatac
      961 actttcacgg tgtcgttttt atccggaacg gcggtggcaa tgcgctcttt cacttccttc
     1021 aggggaatat tgatggcccc ctgaacgtgc tcttgctgat actgctctgg aacacgaaca
     1081 tcgatccagt gttcagcggc gaaaacgggc agtgaaaaca ccagtgccag agcaagtaag
     1141 ccttttttta aacatgtaag cgtccttcta tggatgaaaa ttgccatcca ttatacgcct
     1201 gagatttaac aatttttgcg aaactcccct caccttttat aacgctgtgc cagtttatta
     1261 gcagcccgac ttaacagcgg ttccagcgcc acagccagca acattttcag cggtcggcga
     1321 gcaactgatt ttatcgccca gcccgccaca cccgccgggc catagcgcag tgcggtaaga
     1381 agtaccagct tgcctgctaa tttgaaacca agcttcacct tttgcccggc ctgttgccag
     1441 cgagtattca taactttcct cacagttgac ggaaacggct acgtaacgtg aaagtatcgg
     1501 aagtgacata acgttccatc tcgcgtaaac gcgtttcact tgccgccagt tcacggtcga
     1561 cttcatccag caattcgctg ctggaaggta gctgctcacc aaaggccatg ttgtccggca
     1621 ttggatcaag cgcaaatgac aaaatgatgt aagcaaccag ggtaaacagc gccagaccga
     1681 aaaaaatcga cagcaccacc aggatacgca ccagttttac cggtacatca aaatagttgg
     1741 caatcccggc gcagacgccg cggaccatgc cctgctgtgg aatacgccat aactttttat
     1801 tgagattaat gcccgccatt agcgatccct ccagttcgga tgttctgcat caagaataga
     1861 ttccagcgcc tgaatacgtt cgcgcatccg ttttgcttca tcaaccagtt gcgctaatcg
     1921 ctgctgctca ctttgcgaca attcactgcg accagaacga ttgctgtaat gcagccataa
     1981 ccagatcggt aaaacaaaca gcacaaaaat ggttaacgga atagccagaa atagcgcgct
     2041 cataagtact ccttagacga tgagcggcgg cgcgttcgga cgccgcctgg atgttattat
     2101 tgattgtctt gcttcatttt ggcttttaat tgtgccagtt gttcgctgat tgcatcatcg
     2161 gctttcagtt cggcaaactg atcgtccagc gatttttgtt taccgaagct gtggctttct
     2221 gcttccgctt ccatctggtc aatacgacgt tcgaaagatt cgaaacgagc cattgcttca
     2281 tccagtttgc cactgtccag ctgacgacgc acatcgcgcg acgagtttgc cgcctgatga
     2341 cgtaacatca atgcctgctg gcgagcgcgt gtttcgctca atttgttttc cagctcgcca
     2401 atctctttct tcatgcgtgc cagcgtatcg tccaccagcg tcacttcatg ttccagggac
     2461 ttaatcagat cggtcagttt ctgtttttca attaacgctg cacgtgccag atcctctctc
     2521 tctttcagca gcgccagttc ggctttttcc tgccattcaa cttcacgcgc cgacgcttgt
     2581 tcaatacggc gagtcagctg tttcttttct gccaacgcac gcgccgaagt agaacgtact
     2641 tcaaccagtg tatcttccat ctcctggatc atcagacgca ccagtttctg tggatcttcc
     2701 gctttctcta acagagcgtt gatgttggca ttcacgatgt cggcaaagcg agaaaaaata
     2761 cccataatgt tgtcctcttg atttctgcgt tcaggattgt cctgctgaac tgttaataca
     2821 atttgcgtgc caatttttta tctttttgat ttataaagat ctgattgaag aatcaacagc
     2881 aacatgccag gatgaattag cgaattacac taacaagtgg cgaatttcat catggccgaa
     2941 tacaaagata atttacttgg tgaggcgaac agctttctcg aagtgctgga acaggtttcg
     3001 catctcgcac cgctggacaa accggtgctc atcatcggcg aacgcggcac cggtaaagag
     3061 ttgattgcca gccgcctgca ttatctctcc tcccgttggc aagggccgtt tatttccctt
     3121 aactgcgcgg cgttaaatga aaatctgctg gattccgaac tgtttggtca cgaagcgggg
     3181 gcgtttaccg gtgcgcaaaa acgtcatcct gggagatttg aacgtgccga cggcggtacg
     3241 ctatttcttg atgaactcgc tacggcaccg atgatggtgc aggaaaaatt attgcgtgtg
     3301 attgagtacg gtgaactgga gcgcgttggc ggtagccagc cgttgcaggt gaatgtgcgg
     3361 ttggtatgcg cgacgaatgc cgatctcccg gcgatggtca atgaaggtac ttttcgcgct
     3421 gacctgctcg accggctggc tttcgatgta gtgcaactgc cgcccctgcg cgagcgcgaa
     3481 agcgacatta tgctgatggc agaacacttt gccattcaga tgtgtcggga aatcaagctg
     3541 cctctgttcc cggggtttac ggagcgcgcc agagaaacat tgctgaatta tcgttggccg
     3601 ggaaatattc gtgaattgaa aaacgtggtg gaacgttcag tgtatcgcca cggcaccagc
     3661 gattatccgc ttgatgacat cattattgat ccctttaaac ggcgtccacc tgaagaagcc
     3721 atcgccgttt cagaaaatac ctcgcttccg acgctgccgc tggatttacg cgagtttcag
     3781 atgcagcagg aaaaagagtt gctgcaactc agtttgcaac aggggaaata taaccagaag
     3841 cgcgcggctg aattactggg gttaacttat catcagttcc gcgcgttgtt gaaaaagcac
     3901 cagatttagc gaagttgagc aatacaactt gccggatgcg gcgtgaatgc tttattcggc
     3961 atacataatc aatacgtcgt ttgcaggcct gataagcgca gcgcatcaga cattattgcg
     4021 ttgggctatt aatcgctcag cgcatcctgc aaaattttca ttgccgcatc gaattgcgca
     4081 tccgggatgg tcagcggata caggaagcga atcacgttgc cgtatgcgcc acaggtcagc
     4141 aggagcagcc cctgcgccag cgcgcgttgc tggattttct gtgcaatcgc cgctgacggc
     4201 tcgcccgttt gcggatcgtt aaactctgcc gcaatcatcg accccaggcc gcgtaccgca
     4261 gcaatggccg gaacgctttc tttggcatca atcaacgtgt ttttgagacg ctggcccagt
     4321 tgattcgcgc gttcgcagag tgattctttg tcgataatat tgagcaccgc gtgcgcggca
     4381 gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc cgcccggcgc gggcgcgtcc
     4441 ataatattcg cgttaccgac cacgcccgaa agcggcatcc cgcccgcgag gcttttcgcc
     4501 atcgtcatta agtccggctt atcggcgtaa tgatccatgg caaacagttt accggtacgc
     4561 gcaaagccgc tttgcacttc atcggcaatc atcacaatac cgtgctcgtc gcacaggcgg
     4621 cgaatggcgg caaccagctc ttttggcgca acgttgaaac cgcactcgcc ctgcaccggt
     4681 tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg atttaaacaa gcgttcgatg
     4741 gcgtcgaggg agtcctgtgt tgaaatgccg tgtaaatctg acggataagg tacgtgatac
     4801 accgaaccag ggaacgggcc gaagccgatt ttgtacggcg caacttttcc ggtcagcgcc
     4861 atggtcatat atgtacgacc gtggaagcca ccgctaaacg caatcacgcc aggtcgtccg
     4921 gtatgggcgc gagcaatttt caccgcgttt tccaccgctt ccgcgccggt agtgaagaat
     4981 gcggttttag cctgcccgct cactggggca agggtgttaa ttttctccgc cagcgtgacg
     5041 tagctttcgt acggcacaat ctgatacgcc gtgtgggtaa actgttgcaa ttgctgctcc
     5101 accgccgcga ccagatcagg gtggcgatgc ccggtattca gcaccgcaat gcctgcggcg
     5161 aaatcgatgt attcgttgcc ctctacgtcc ttcagcgtag cgttttctgc cgactgggcg
     5221 aagaagttac acatcacgcc aaccccgcgt ggggtagcag aaagacgacg ctgatggaat
     5281 tcattgttgc tcatgtttca caccttttga ctcttcactg ttaatcggaa attaaacgcc
     5341 cagacgatcg cgcaggctgt aataagccgc gcccatcgcg gtaaatggca cgcgcagcgt
     5401 gcgcccgccg gggaacgggt aatgcggcag attggcgaag gcatcgaaac gctcggcgtc
     5461 accgcgcagc agttcagcaa tcaaacgccc ggcaagatgg gtacaggtga cgccgtggcc
     5521 gctgtaaccc tgcatgtaat agatgttggt atcaaggcgg ccgaactgcg gcatgtgcga
     5581 caaggtcagc aggaagttgc ccgtccagcg gtaatcaatt ttcacgccct tcaactgagg
     5641 gaaggttttt agtagtttcg gtaccacaag acgctcaacg tcatccgggt tgcgtgcacc
     5701 atacactaca cccccgccgt acagcaggcg gttgtcggcg gtaagacggt agtaatcaag
     5761 cagatagtta cagtcttcca cacagtagtt tttcgggatc agcgaacggg ctaaatcttc
     5821 cgacagccgt tcggtggtga tcacctgcgt gccgcacggc atgctgcgtt tcgctagttc
     5881 cggctctact ttatcgccca gatacgcatt cccggcgaca atcacatact tcgccgtcac
     5941 ctgaccattg gcagtccgaa ccacggctgg cgtggtgtgc tggatctgcg tcacggcgga
     6001 aagctcatac acgcgcccgc cgttcaggcg gatggcatcg gcttcaccaa tggcgaggtt
     6061 tagcgggtga atatgcccac cgctgtgatc cagcagcgca ccagtgtagc gatcgctggc
     6121 gacctcgcgg cgaatagcgt tgacgtccag cagctccagc tgtttattgc cgtagcgtac
     6181 ccagttctct ttctgctctt cgagtgtggc gagttgttta tcattcatcg ccacaaacag
     6241 accgccgggg cggtagtcgc agtcaatctg ataacgtttg atacgttcgc ggatgatctc
     6301 gccaccctcg aacatcatgc tgccgagcat acgggcggtg tccatgccgt agcttttttc
     6361 gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc ccgccattgc gcccgcttgc
     6421 gccaaagccg atgcgtgagg cttcgagaac cactacgtca aagcccgctt ccgccagatg
     6481 cagcgcggag gagagcccgg tatagccgcc gccaaccacg caaacgtcgc aggtgattga
     6541 ctcattcagt gtgtcgaatg gtgcatattt attcgcactg gcggcgtagt aactgctggt
     6601 atgttcggtc atttcaggcc tccaggctta tccagatggt tttcagttca gtgaattttt
     6661 caagggcatg cagggatttg tcgcgaccgt tgccgctctg cttatagccg ccaaacggca
     6721 cggtcatatc gccgtcgttg tagttattga cgaagacgga accggctttc aggcgtcggc
     6781 tcatgcggtg cgcgcgggag aggtcgcgcg tccataccgc cgcgccaagg ccgtactggc
     6841 tgtcgttggc aagctgcagc gcctgatctt ctgatgtgaa acgggtgacc accagcaccg
     6901 gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc cacatccacg aagatggtcg
     6961 ggccgatggc cgctgccagc ccggcgttgc gaccatccag cagcagttgc cccttgcctt
     7021 cgccttcacg aataaaggtg tgtaccgagt cagcgtgagc gctgtcgatt aaggtgccca
     7081 tggtggttgc gggatcaagt ggatggcccg gctgccagtt ttgcgcctgc tgttttaaca
     7141 gggctaagaa ttcatcggcg atgctctctt ccagtaacaa acgcgttccg gcgatgcgca
     7201 cctggccctg gttgtagaag atgcctgcgg cggtggcgct tgccgctttt tgcagatccg
     7261 gacaatcggc gaaaacgatg ttggcgcttt tgccgcccgc ttccagccag acacgtttca
     7321 tgttgctatc acccgcgtct ttcagcagtt gtttacccgt acgcgtggag ccggtaaagg
     7381 cgatggcatc gatatcgtta tgacgcgaaa gcgcctgccc cgcttcatga ccaaaacccg
     7441 tcaccacgtt caacacacca tccggcaagc ctgcttcttt cgccagcccc gcgagacgaa
     7501 tcgcactgag cggtgatttt tcagacggtt ttagaatcac gctgtttccc gccgccagcg
     7561 ccgggccgag tttccagcaa gtcagcaaca gcgggaagtt ccacggcacg atggcggcaa
     7621 tcacgccgac cggttcacgc acgatcatcg ccagctcatg gctactggtg gtcgccactt
     7681 cgccatacac tttgtcgatc gcttcggcgt accagcgaat ggcgcgcgcc gcgccgggaa
     7741 tatcatcacg cagactgtga cgaatcggtt tgccggtgtc gagagtttcc agcagtgcca
     7801 gctcttcggc gtgggcttcc attaaatcgg cgagtttatt cagtaccgct ttacgtttag
     7861 ccggagaaga gagtgaccag tcgccgcgtt caaatacgcc gcgtgctgcg ctcaccgcac
     7921 ggtcgatatc gacgctcttg ccgcgggcaa ttttcgccag cggtgcctgg gtgaccggat
     7981 caacggtttc aaaggtttca ttttccgccg cagcagtata ttcaccgtta ataaataagc
     8041 ggttttcaat ggcgagactt aacgctttat cctgccagta agccagatga tgaaaattca
     8101 ttatgactcc tgtttcacgt ctatcagata tatgcagttt gtgaacgtca gggcgcagag
     8161 atccccgggc tgcatgcccg tttatttctg gcgacattct ttatggattg ccgggcgcat
     8221 tgcgcttacc cggcttacag aacaaaaaga ttaaaacgtg gtgggcgtat gggcgctgat
     8281 aattcggcaa atacctgccg acgtattact gaaactgtgc gggatgccgg tattaatggc
     8341 ataactttgc cccgcgacga ggtggtaatc ctgaccatta atcgtcagaa caatttcacc
     8401 ttccagtact gtgcctattt cctcaccctg atgcttaatt ctttccccag tggttgtgcc
     8461 cggctggtac gtttcaaaga tcatcgccag cgtgcgattc gggttaccgt tatgaaccag
     8521 cttcattgac acaccctgac tgcccatctc aattaagtcg tcctgattaa tgacgacctg
     8581 cggctcatca ggtttttccg gctcggaaaa gaattccgag agtgacagac catacacctt
     8641 cagcagcttt tgcagcgtac tgatggcagg gctgacttta tcttgttcta tcgtactgat
     8701 agcactgtga gtcagcccgg agagttcggc ggcacgacgt tgtgaaagcc cctgctgctg
     8761 gcggatttcc gacaagcgtt ttcctggcgc cagtccctca tcactcatat ttgcatttcc
     8821 ttaaactgta gtggtcagag tcgctgtttt tcagcgatat ggtgctgcca agcggtgata
     8881 aagccctcga acaatatacg cgaaagcgcg tactcgctac tgttccattc cgggtgccac
     8941 tgtacgccca gcgcaaaagg atgattgatg acgctaaccg cctcaactaa gccatccggc
     9001 gagcgagctt caacacgcaa ccgtgggcta acgaccttcg ccccttgtcc atgtagagag
     9061 tttacccaaa agttgctaca ttcaggtaac aacgcagaca gtaatccccc ctcttcaacc
     9121 tgaacttcat gcgacggtgc atattgctgt tccaccggca gttcgggatc ttcccgatgt
     9181 tccagcaatt caggctgttc gcacagcttg cgatgcaacg aaccaccggt tgccaccacc
     9241 agttcttgta aaccccggca gatggcgaaa atggggatgc gcctttcgag tgcggcatta
     9301 attatcgcca tgctcagaag atcacgcccg ggatcggcgt caggctcatc gccgttttca
     9361 ccatataggt gcggctgcac attgctggga ctactaggaa gataaatgcc atcgagtttc
     9421 ggcaaaagtt gttcaagtaa cgacggttcc gccagcgcat gtggtagcgc aattggcaag
     9481 ccgcctgcgt ggatgatggc attcaggtac ttttcttgca gagtctgggt cgcatgaccc
     9541 ttaagcctgt tcctgcacat tacgacaccg ataaccggat tgttcattat attttccatg
     9601 ctcaatctca caaaatggac taaattatcg ccattactgc gctttaaatg acctttatgt
     9661 tcaatatttt ttcaatctag cagtggattt tcatttttgc aaactcaatt taacatttga
     9721 caaacatttg gtttgcatac agattcgaat gatggtcatt atattttacg ctttgataac
     9781 gagcggaaaa caaaccaaag gcgaagaatc atggaaacca atatcgttga agtagagaac
     9841 tttgttcagc agtcagaaga gaggcggggt agcgccttta cgcaggaagt gaaacgctac
     9901 ctggagcgct acccgaatac gcaatatgtt gatgttttac ttactgattt gaatggttgc
     9961 ttccgtggaa aacgtatccc tgtttcaagc ctgaagaagc tcgagaaagg gtgttatttc
    10021 ccggcctcgg tatttgcaat ggatattctg ggcaacgtag tagaagaagc aggtctgggt
    10081 caggaaatgg gcgagccgga tcgtacctgt gttcctgttc tcggttcctt aactccttct
    10141 gccgccgatc cagagtttat cggtcagatg ctcctgacca tggtcgatga agatggcgct
    10201 ccctttgacg ttgagccgcg gaacgttctt aaccgcctgt ggcagcagct gcgccagcgc
    10261 ggattgttcc cggttgtagc ggtagagctg gagttctatt tactggatcg ccagcgcgac
    10321 gctgaagggg atctgcaacc gccctgcgcg cctggcaccg atgaccgcaa tacacaaagc
    10381 caggtttact ccgttgataa cctcaaccac ttcgccgacg tgctcaatga tattgatgaa
    10441 ctggcgcagt tgcagctgat tccggcagat ggcgcggtcg ctgaggcctc gccgggtcag
    10501 tttgaaatca acctttacca tactgataac gtgctggaag cctgcgatga tgcgctggca
    10561 ctaaaacgcc tagtgcgtct gatggcagaa aagcataaga tgcacgccac ttttatggcg
    10621 aagccgtatg aagagcacgc gggcagcggg atgcatatcc atatcagtat gcaaaataat
    10681 cgtggcgaga atgtgctttc tgacacagaa ggcgaagatt cgccgctgct gaaaaagatg
    10741 ctcgccggga tgattgacct gatgccgtcg tcgatggcgt tgctggcacc aaacgtgaac
    10801 tcgtatcgcc gcttccagcc gggaatgtat gtgccgacgc aggcgtcgtg gggccataac
    10861 aaccgcaccg tcgccctgcg tattccgtgc ggtgaccgtc ataatcaccg cgtggaatat
    10921 cacgtggcgg gtgccgatgc caacccatat ctggtgatgg cagcgatttt tgccggtatt
    10981 ttgcatggcc ttgataacga gctgccgttg caggaagaag tcgaaggcaa cgggctggaa
    11041 caggaaggct tacccttccc gattcgccag agcgatgccc tgggtgagtt tatcgagaat
    11101 gatcacctgc gccgctattt aggcgaacgg ttttgccatg tgtatcacgc ctgtaaaaat
    11161 gatgagctgt tgcagtttga gcgcctcatc acagaaacag aaattgagtg gatgttgaaa
    11221 aacgcgtaat tccccttgcg gggttgtttc atcggcctga gcggagccag gcctgacatt
    11281 tgctggtaat ccagacattt cccggatgtc gcgttatgga gcgcaggcgg caacgggctt
    11341 ccgttaacga ttaagactgt gacgaggaaa tgagatgatg ccgatgatta aatccccgca
    11401 tggcgaggga ggctgcgtat gcgcccctcc tgctactgac tggactccgc cccctttgct
    11461 tcccttgcta aacaggtttg atttcaggtc gacacgaccg caaaccttat tacgcagggg
    11521 aggcagcaat tatggctatt aattcaccac tgaatattgc tgcgcaaccc ggcaaaaccc
    11581 gtctgcgaaa atcactgaaa ttgtggcagg tggtaatgat gggtctggcc tatctcacgc
    11641 cgatgaccgt atttgatact tttggtattg tctccggcat tagcgacggt cacgttccgg
    11701 cgtcctattt gctggcgctg gcgggcgtgc tgtttaccgc tatcagctac ggcaaactgg
    11761 ttcgccagtt tccggaggcc ggttcggcct atacctacgc gcaaaagtcg attaacccgc
    11821 acgtcggatt tatggtcggc tggtcatcac tgctggatta tctctttttg ccaatgatca
    11881 acgttttgtt ggcgaaaatc tatctctccg ccctcttccc ggaagtgccg ccgtgggtgt
    11941 gggtggtaac cttcgtcgcc attttaaccg ccgcgaatct gaagagcgtc aacctggtcg
    12001 ctaacttcaa taccctgttt gtactggtgc aaatctccat catggtggtg tttatcttcc
    12061 tggtggttca gggactgcat aaaggagaag gcgttggcac cgtctggtca cttcagccgt
    12121 ttatcagcga gaacgcgcac ctgatcccga ttattaccgg tgcgacgatt gtctgtttct
    12181 cgttcctcgg tttcgatgcg gtgaccacgc tttcggaaga gacgccggac gccgcacgcg
    12241 tgatcccgaa agccatcttc ctgacggcgg tatacggcgg cgttatcttt atcgcggcgt
    12301 cgttctttat gcagctgttc tttcccgata tcagccgctt taaagacccg gacgccgcac
    12361 tgcctgaaat tgcgctgtac gtcggcggca agctctttca gtcgattttc ctctgcacca
    12421 cgtttgtgaa cacattagcg tctggcctgg cttcacatgc cagcgtgtcg cgtctgctgt
    12481 atgtgatggg gcgtgacaat gtgtttccgg agcgcgtgtt tggctatgtg cacccaaaat
    12541 ggcggactcc ggcactcaac gtcattatgg tcgggattgt cgcgctgtcg gcgctgttct
    12601 ttgatttagt caccgcgaca gcactgatta acttcggtgc tctggtggcg tttaccttcg
    12661 tgaatctgtc ggtgtttaat catttctggc ggcgtaaagg aatgaataaa agctggaagg
    12721 atcacttcca ctatttgctg atgccgctgg ttggcgcgct gacggtgggt gtgctgtggg
    12781 ttaacctaga gtcaacgtca ctgacactcg gtctggtatg ggcttcgtta ggcggcgcat
    12841 atttgtggta tctgatccgc cgctatcgca aagtgccgct gtacgaaggt gacagaacgc
    12901 cggtgagcga aacgtaatag ccggatgcgc ggcgcattat cctgcctgag ggtaacgcgc
    12961 cgcgccatta actataaaaa gatgtatgac ccggatcacg agtgctatca tccgccctta
    13021 tgcttagcgc attaacccca ggggcaaata atgaaccacg atatcccgct aaaatatttt
    13081 gatattgccg atgagtacgc gaccgaatgc gcagagcctg tcgcagaggc tgaacgcaca
    13141 ccgctggccc actacttcca gttactgctc acccgtttga tgaacaatga agagatcagc
    13201 gaagaagccc aacatgaaat ggctgctgaa gcggggatta atcccgtacg cattgatgag
    13261 atcgcagagt ttttgaatca atggggtaat gagtaattat ttaattgcag acgatcggtc
    13321 agtatcgttg tccggataaa gcgttcacgc cgcatccggc taacaatgac acgcactgca
    13381 gcgtgcttta atgggtttct cacgatcgta ccgctctgct cctcgctaag ttgttctgtg
    13441 agttatagtt atagagaaat cacgggtacg aatgttgaac agatggcgac gccacctgtt
    13501 caacgaccaa ccacacggcc ctcattacga cccctgaaca caaacggtaa tggcaaatgt
    13561 cattaaatat tagtaagata gcctctttac ataaaatccc ttaatattat gcgccaggta
    13621 ttatcgtctc ttttggtgat tgctggactt gtgagtggtc aggcaatcgc cgcgcctgaa
    13681 tcccccccgc atgctgatat ccgcgacagc ggttttgtct attgcgtcag cgggcaagtc
    13741 aacaccttta acccatccaa agcgagcagt gggttaattg tcgataccct tgccgcccag
    13801 ttttatgatc gactgctgga tgtcgatccc tatacctatc gcctgatgcc ggaacttgcc
    13861 gaaagctggg aagtactcga caacggcgcg acctatcgct tccacctgcg tcgcgatgtt
    13921 ccgtttcaaa aaaccgactg gtttactccc actcgtaaaa tgaatgccga cgatgtggtg
    13981 tttaccttcc agcgaatttt tgaccgcaac aacccgtggc ataacgtcaa cggcagcaac
    14041 ttcccctact tcgacagcct gcaatttgcc gataacgtga aaagcgtccg caaactggat
    14101 aatcataccg ttgagttccg actcgcccag ccggatgctt cttttttgtg gcacctcgca
    14161 acccattatg cttcggtcat gtcggcagaa tatgcccgga agttagagaa agaagatcgc
    14221 caggagcaac tcgaccgtca accggtcggc accggtccgt atcagttgtc ggaataccgc
    14281 gccgggcaat ttattcgcct acaacgtcat gatgacttct ggcgcggtaa accgttaatg
    14341 ccgcaggtag tggtggattt aggctccggc ggcaccggac gtctgtcgaa actcctgacc
    14401 ggggaatgcg acgttctggc ctggcctgct gccagccagc tatccatttt gcgtgacgac
    14461 ccgcgcttgc gtttaacgct gcgtcctggg atgaacgtcg cctatctggc atttaacacc
    14521 gccaaaccgc cgctaaataa tcccgctgtc cgccatgcac tggcactggc gattaataac
    14581 cagcgcctga tgcaatccat ctattatggt acggctgaaa cggcggcctc tattttaccg
    14641 cgcgcctcgt gggcctatga caacgaggct aaaattactg aatacaatcc ggcgaaatcg
    14701 cgcgaacagt tgaaggcgtt ggggctggaa aacttaacgc tgaaactgtg ggtgcccaca
    14761 cgttcgcagg cgtggaaccc cagtccactg aaaactgccg aactgattca ggcggatatg
    14821 gcgcaggttg gcgtaaaagt ggtgattgtg ccggtagaag gtcgctttca ggaggcgcgg
    14881 ttgatggata tgagccatga tctgacgtta tccggttggg cgacggacag taacgacccg
    14941 gacagtttct tccgtccgtt actgagctgc gcggcaattc attcacagac caacctcgcc
    15001 cactggtgca atccgaaatt cgacagcgtg ttgcgtaagg cgctctcctc gcagcagctg
    15061 gcagcgcgta ttgaagccta tgacgaagcg cagagtattc tggcgcagga attacctatt
    15121 ctgccgctgg cgtcgtcatt gcgtttgcag gcctatcggt acgatatcaa aggtctggta
    15181 cttagcccgt ttggtaacgc ctcctttgct ggggtgtatc gcgagaaaca ggatgaggtg
    15241 aaaaaaccat gattatcttc accttacgcc gcattttgtt attgattgtc accttgttcc
    15301 tgctgacctt cgttggcttc agcttgagct atttcactcc acacgcgccg ttacaaggcg
    15361 cgtcactgtg gaatgcctgg gtgttctggt ttaatggcct gatccactgg gattttggtg
    15421 tttccagcat taacggtcaa ccaattgccg aacagttaaa agagctcttc ccggcgacga
    15481 tagaactgtg catcctcgcc ttcggttttg cgctgattgt tggcatcccc gtggggatga
    15541 ttgccgggat tacgcgccat aagtggcagg ataacttgat caacgccatc gccttactgg
    15601 gcttttcaat cccggtgttc tggctggcgc ttctgttgac actgttttgt tcactcacgc
    15661 taggttggtt gcccgtttca gggcgtttcg atctgctcta cgaagtgaaa ccgattaccg
    15721 gttttgcgtt gattgatgcc tggctttcgg actcaccatg gcgggatgaa atgatcatga
    15781 gcgcaatccg ccatatgata ttgcccgtga tcaccctgtc ggttgcgcca acaactgaag
    15841 tgatccgcct gatgcgtatc agcactattg aagtgtatga ccagaactat gtaaaagcgg
    15901 cggcgacccg cggtttgtcg cgctttacta ttttgcgtcg ccacgttctg cataacgcgt
    15961 taccgccggt tattcctcgc ctgggcttac agttttccac catgctgacg ctggcgatga
    16021 tcaccgaaat ggtttttagc tggccgggtt tagggcgctg gttaattaac gccatccgcc
    16081 agcaggatta cgcagccatt tccgccggag tgatggtgtg tggctcactg gtcattattg
    16141 ttaacgtgat ttctgatatt ttgggtgcca tggctaaccc tctgaaacat aaggaatggt
    16201 atgccttacg atagcgtata cagcgaaaag cgcccgccgg gcacgctgcg taccgcctgg
    16261 cgcaaatttt atagtgacgc ctctgcgatg gtcggcctgt acggctgcgc gggactggct
    16321 gtactgtgta tttttggcgg ctggtttgcg ccttacggca tcgaccagca atttctcggt
    16381 tatcaattat tgccgccatc atggtcgcgc tatggcgaag tttctttctt cctggggact
    16441 gacgacctcg gacgtgatgt tttaagccgt ttactgagcg gagctgcgcc aaccgtgggt
    16501 ggcgcatttg tggtgacgct tgccgcgacg atctgtggcc tggtactggg caccttcgcc
    16561 ggggcgacac acggcctccg ctcagcggtg cttaaccata ttctggatac cttactggcg
    16621 atcccttcgc tgctgttggc aattatcgtt gtagcgtttg ccggaccgag tttgtcccac
    16681 gctatgtttg ctgtctggct ggcattgctg ccgcgtatgg tacgttcgat ttacagcatg
    16741 gtgcatgacg aactggaaaa agagtacgtt atcgccgccc gtctggatgg cgcatcaacg
    16801 ctgaatattc tctggtttgc tgtgatgcca aacatcaccg ccgggctggt caccgagatc
    16861 acccgcgcac tgtcgatggc aattctcgat atcgccgcgc tgggctttct cgatctcggc
    16921 gcacaactcc cctcgcctga atggggagcg atgctcggtg atgcgctgga actgatttat
    16981 gtcgccccgt ggactgtcat gctgccaggt gcggcaatta tgattagcgt gttgttggtt
    17041 aacctccttg gcgatggcgt tcgtcgtgcg attattgcgg gggtggaata atgccattac
    17101 tcgatattcg taacctgacc attgaattta aaaccggtga tgagtgggtt aaagccgtcg
    17161 accgggtaag tatgacctta accgaaggtg aaatccgcgg tcttgttggt gaatccggtt
    17221 ccggcaagag tttgattgcg aaagcgattt gtggggtgaa taaagataac tggcgtgtta
    17281 ctgctgaccg tatgcgtttt gatgatatcg atttgctgcg tctctccgca cgcgaacggc
    17341 gcaaactggt tggccataac gtgtcgatga ttttccagga accgcagtcg tgtcttgacc
    17401 cttcagaacg tgtgggtcgc cagttgatgc aaaacatccc agcctggacc tataaaggcc
    17461 gttggtggca gcgttttggc tggcgcaaac gccgtgccat tgaactgctg caccgcgtgg
    17521 ggattaaaga tcacaaagat gcgatgcgca gttttcccta tgagttgacc gaaggtgaat
    17581 gtcagaaagt gatgatagcc attgcactgg cgaatcaacc gcgtctgctg attgctgacg
    17641 aaccgacaaa ctcaatggag ccaacaaccc aggcgcaaat ctttcgcctg ctgacgcgtc
    17701 tcaaccaaaa cagcaatacc actattttgc ttatcagcca tgacttacaa atgcttagcc
    17761 aatgggcgga taaaattaac gtgctttact gcggtcaaac agtggaaacc gcgccgagta
    17821 aggagttggt gacgatgcca catcatcctt atacccaggc gctgatccgc gcgataccag
    17881 acttcggcag cgcgatgccg cataaaagtc gcctcaatac tctacccggc gctatcccat
    17941 tgctggaaca gttaccgatt ggttgtcgtc tggggccacg ttgcccgtat gcacaacgag
    18001 aatgcattgt gacgccacgt ttgacggggg cgaaaaatca tctctatgcc tgtcatttcc
    18061 cgctgaacat ggagaaagag tgagatgatc gaaacgctgc ttgaagtgcg taatttaagt
    18121 aaaacgttcc gctaccggac cggttggttt cgtcgtcaga ccgtagaagc ggtaaaaccc
    18181 ttgagcttta cgctacgtga aggccagaca ctggcgatta ttggcgagaa tggttcgggt
    18241 aaatccacac tggcaaaaat gctggcggga atgatagaac ctaccagcgg cgagttattg
    18301 attgacgatc atccgctgca ttttggcgat tattccttcc gtagtcagcg tattcgcatg
    18361 atttttcagg atccttcgac ctcgttgaat ccccgtcaac gtatctcaca aattctggat
    18421 tttccactgc gcctgaacac cgacctggaa ccggaacagc gtcgcaaaca gattattgaa
    18481 acgatgcgta tggttgggct gctaccggat cacgtcagtt actatccgca tatgttggca
    18541 cccggacaaa aacagcgtct gggtctggcg cgcgcgctga tattgcgccc aaaagtcatt
    18601 attgccgatg aagccctcgc ttcactggat atgtcaatgc gttcgcagtt gattaatctg
    18661 atgctggagt tacaggaaaa acagggcatt tcgtatattt atgttaccca gcatatcgga
    18721 atgatgaagc acattagcga ccaggtgctg gtgatgcatc agggcgaggt tgtcgagcga
    18781 ggcagcaccg cggatgtgct ggcatcgccg ctgcatgaac tcaccaaacg gctgattgcc
    18841 gggcactttg gcgaagcatt gacagcggat gcgtggcgta aagatcgcta attttctgaa
    18901 tcctccttcc agattattca cctcattctg acaagagatt aaatcttgtc agaatgaggt
    18961 gatggataaa ataaaatcaa atgcgcgtga tttaagacgc aatctcactt tgcaggaacg
    19021 aaagctctgg cgatatcttc gcagccgacg ttttggtgat ttcaaatttc gccgtcaaca
    19081 tccggtgggg agctacattc tcgattttgc ttgctgctcg gcgcgcgtag tcgttgagct
    19141 ggatggtggg cagcatgatt tggcagttgc ctatgatacc aggcgcacta gctggcttga
    19201 gtcgcagggc tggaccgtgc tgcgtttctg gaataacgag attgattgta atgaggaggc
    19261 ggtgctggag attattctgc aggaactgaa ccgccggtca ccctcaccct gaaagggcga
    19321 gggggccgac cgagccgaat agctgttgtg gtgaaaacat ggagacagtg ctggagaata
    19381 ttcggcaagg tctgaaccat cccagccatc gccataaaag ggttaggggc tgtatgagcc
    19441 tgtttgttgc tggggtaaca atatttgcac aatatggtcc cctcgcccct ctggggagag
    19501 ggttagggta aggggaaaag cgccccccct gccgcagcct gctccggtcg gacctggtaa
    19561 ctatagctac tcacagccag gttgattata ataaccgttt atctgttcgt actgtttact
    19621 aaaacgacga atcgcctgat tttcaggcac aacaagcatc aacaataagg attaaagcta
    19681 tgggttttct ttccggtaag cgcattctgg taaccggtgt tgccagcaaa ctgtccatcg
    19741 cctacggtat cgctcaggcg atgcaccgcg aaggagctga actggcattc acctaccaga
    19801 acgacaaact gaaaggccgc gtagaagaat ttgccgctca attgggttct gacatcgttc
    19861 tgcagtgcga tgttgcagaa gatgccagca tcgacaccat gttcgctgaa ctggggaaag
    19921 tttggccgaa atttgacggt ttcgtacact ctattggttt tgcacctggc gatcagctgg
    19981 atggtgacta tgttaacgcc gttacccgtg aaagcttcaa aattgcccac gacatcagct
    20041 cctacagctt cgttgcaatg gcaaaagctt gccgctccat gctgaatccg ggttctgccc
    20101 tgctgaccct ttcctacctt ggcgctgagc gcgctatccc gaactacaac gttatgggtc
    20161 tggcaaaagc gtctctggaa gcgaacgtgc gctatatggc gaacgcgatg ggtccggaag
    20221 gtgtgcgtgt taacgccatc tctgctggtc cgatccgtac tctggcggcc tccggtatca
    20281 aagacttccg caaaatgctg gctcattgcg aagccgttac cccgattcgc cgtacagtta
    20341 ctattgaaga tgtgggtaac tctgcggcat tcctgtgctc cgatctctct gccggtatct
    20401 ccggtgaagt agtccacgtt gacggcggtt tcagcatcgc tgcaatgaac gaactcgaac
    20461 tgaaataatc gttctgttgg taaagatggg cggcgttctg ccgcccgtta tctctgttat
    20521 acctttctga tatttgttat cgccgatccg tctttctccc cttcccgcct tgcgtcagga
    20581 taacgatttc ctttacgacc aaggagcgcc catggaacaa cgccacatca ccggcaaaag
    20641 ccactggtat catgaaacgc aatccagtac tacggagtat gacgttctgc ctctggtccc
    20701 ggaagccgca aaggtcagcg atccctttct gctcgacgtg atccttgatg aagagacgct
    20761 ggcccccttc ctttcatggc tggtccctgc gcgcgttctt gcagtggagt tgttccctga
    20821 ccagcttacc gtgacccgtt cacaaacctt caccgcttat gaacgcttgt cgacggccct
    20881 gacggttgct caggtttgcg gcgtccagcg gttatgtaac tactattcgg cgcgacttac
    20941 gccgctcccc ggacctgatt cctccaggga aagtaatcat cggttggcac aaatcacgca
    21001 atatgcccgc caactggcta gctcgccttc tattatcgac aaccgatcgc gccagcatct
    21061 gaatgacgtc ggtcttactg tctgggactg tgtgatcatt aaccaaatca ttggttttat
    21121 tggctttcag gcgcggacaa ttgcgacatt tcaggcttat ctcgggcatc cggtacgctg
    21181 gttacccggg ctggcgatac aaaactacgc cgacgcgtca ctgtttgctg atgaatcaat
    21241 acgctggcga agcagctatg aggtggaaaa actacctgaa gagcacacaa aaagttcaac
    21301 tgcagaactt tgccaactgg ccgaaacact ctctctccac cctatttcac tttcccttct
    21361 cgaaaagttg ttaaacagca cacgggtcaa tgcacagccg gataatcagc ttgcggcgtt
    21421 gttatgcgcg cgtataaatg gcagtcctgc ttgtttttcc acctgtatgg attcatcaaa
    21481 tgaatataaa aaaatcagca cccttctgcg caagggcgaa aatgaaatta accgatgggc
    21541 tgaccgtcat tctgttgagc gcgctaccgt tcaggcgata caatggctga cccgagcacc
    21601 cgatcgcttt agcgccgccc agttcagccc tttactcgaa cacgaaaaat catcaacgca
    21661 gattattaat ctgctggtat ggagcgggct gtgtggctgg ataaatcgct taaaaatcgc
    21721 gttgggtgag acatattaac cttgccgcgt cagacagatt cgcgtaaaac tgtcagccgc
    21781 tctaatggcc accaaaatag acaattatgt ttcaggacaa cccgctgcta gcgcagctta
    21841 aacagcaact gcattcccag acgccacgcg ctgaaggggt ggtaaaagcc acagaaaaag
    21901 gctttggctt cctggaagtc gacgcacaaa aaagttattt cattccgccg ccgcagatga
    21961 aaaaagtcat gcatggcgac cgaattatcg cggtgatcca cagtgaaaaa gaacgtgaat
    22021 ccgcagagcc agaagaactg gttgaaccgt tcctgactcg tttcgtgggt aaagttcagg
    22081 gcaaaaatga ccgtctgacc atcgttcctg atcatccact tttaaaagac gccattcctt
    22141 gccgcgcagc ccgtggcctg aaccacgagt ttaaagaagg cgactgggcg gttgccgaaa
    22201 tgcgccgtca tccgctgaaa ggcgatcgtt ctttctatgc tgaactgaca caatacatca
    22261 cttttggtga cgaccacttt gtaccgtggt gggttaccct tgcgcgccat aatctggaaa
    22321 aagaagcacc agacggcgtc gctaccgaaa tgctcgatga aggtctggtt cgtgaagatc
    22381 tgacctcgct ggattttgtc accatcgaca gtgccagcac tgaagatatg gatgacgccc
    22441 ttttcgctaa ggcgctgccg gatgacaaac ttcagctgat tgtggcgatt gccgatccaa
    22501 ccgcgtggat tgctgaaggc agcaagctgg acaaagccgc gaaaattcgc gcattcacca
    22561 actatctgcc tggcttcaac atccctatgc tgcctcgcga gctttctgac gatctctgct
    22621 cactgcgcgc caatgaagtc cgcccggtac tggcatgccg catgacgctc tccgctgatg
    22681 gcaccattga agataatatc gaattctttg ccgccaccat cgaatccaaa gcgaagctgg
    22741 tgtatgacca ggtttctgac tggctggaaa ataccggtga ctggaagcct gaaagtgaag
    22801 caattgccga acaagtccgt ttgctagcgc aaatttgcca acgccgcggc gagtggcgtc
    22861 ataaccacgc actggtgttt aaagatcgcc cggattaccg ctttattctc ggtgaaaaag
    22921 gtgaagtgct ggatatcgtc gccgagcctc gtcgcattgc caaccgtatc gtcgaagaag
    22981 cgatgattgc cgctaacatt tgtgcggccc gcgtactgcg cgataagctc ggttttggca
    23041 tctataacgt gcatatgggc tttgatccgg cgaatgccga cgcgctggca gcgttgctga
    23101 aaacgcacgg tctgcatgtc gatgccgaag aagtgctcac gctggacggt ttctgcaaac
    23161 tgcgtcgtga actggatgcg caaccaactg gtttcctcga cagccgcatt cgtcgcttcc
    23221 agtcatttgc tgaaattagc tctgaacccg gtcctcactt tggcctcggt ctggaagcat
    23281 acgccacctg gacttcgccg atccgtaaat atggcgacat gatcaaccac cgtctgctga
    23341 aagcggttat caaaggcgaa actgcgacgc gtccgcagga tgaaatcact gtccaaatgg
    23401 ccgaacgtcg ccgtctcaac cggatggcag aacgtgatgt tggcgactgg ttatacgcac
    23461 gcttcctgaa agataaagcc gggaccggca cccgtttcgc ggcggaaatt gtcgatatca
    23521 gccgtggcgg catgcgtgtg cgtttggttg ataacggcgc tatcgccttt attccggcac
    23581 ctttcttaca cgctgtgcgc gatgaactgg tttgcagcca ggaaaacggc actgtacaaa
    23641 ttaaaggtga aacggtttac aaagtaactg acgttattga cgtcaccatt gccgaagtcc
    23701 gcatggaaac ccgcagcatt attgcgcgcc cggtcgcgta atctcctttc acggcccatt
    23761 cctcatggat gggccgttta tttccccgct ctaccttcgt tatatccagg cagatttttt
    23821 aactgacttt cgtttgaaaa ctggcgtttt tccaaaactg gattacaaat aaatatataa
    23881 aaaaacaatg cgtttagccg ccgttaattt catgtaagtc atgttgttcc ttttatttaa
    23941 ccgcggactc cgctgttaac cggaggatat gcatcatgaa aaccgttagg gagtccacaa
    24001 cgttgtacaa ctttctcgga tcgcacaatc catactggcg gttgacggaa agcagcgatg
    24061 ctttgcgctt ttctaccacc aaaaccacag aacctgatcg tacattgcag ttatctgccg
    24121 aacaggctgc tcgcatcagg gaaatgacgg tcatcacctc cagcctgatg atgagtctga
    24181 ccgtcgatga aagcgatctt tctgtgcatc tggtaggacg aaaaatcaat aaacgggaat
    24241 gggctggcaa cgcgtctgca tggcatgaca caccggcggt tgctcgtgat ttatcacacg
    24301 ggctttcctt tgctgagcag gtagtttctg aagcacattc cgcaatagtg attctcgaca
    24361 gccgggggaa tatccaacgc ttcaatcggt tatgtgaaga ttacacaggg ttgaaagaac
    24421 acgacgtcat tgggtaaagc gtgtttaaac tgtttatgag ccgtcgtgaa gctgcggcat
    24481 ccaggcgcaa taaccgtgta ttttttcgaa gcggcaatgc atatgaagtc gaactgtgga
    24541 taccaacacg taaaggccag cggctgtttc tgtttcgcaa taaatttgtc cacagcggca
    24601 gtggcaaaaa cgagattttt ttaatctgtt ccggtaccga cattaccgaa gagcgccgca
    24661 ctcaggagcg actgcgtatt ctggcaaata ccgacagtat caccggactg ccgaatcgta
    24721 acgcaatgca ggagttaatc gatcacgcta ttaatcaggc agataacaat aaagttgggg
    24781 ttgtgtatct tgatttggat aattttaaaa aggtcaacga cgcctatggg catttgtttg
    24841 gtgaccagtt attacgcgac gtgtcattgg ctattttaag ctgtctcgaa catgaccagg
    24901 tgttggcgcg tccaggtggc gatgagtttc tggtactggc atccaatacc tcacaaagcg
    24961 cgctggaagc aatggcatca cgaattttga cccgcttacg cctccccttt cgtattggtt
    25021 taattgaagt ttataccagc tgttcagtag gtatttcgct ctctcccgaa catggttcgg
    25081 acagcgcgac tattattcgt cacgccgaca cagcaatgta cacagcgaag gaaggcggac
    25141 gaggacaatt ttgcgttttt actccagaaa tgaatcagcg ggtatttgaa tatctctggc
    25201 tggataccaa cttgcgtaaa gcactggaaa acgatcagtt ggttatcgaa aatcacctag
    25261 cgtgtcgaag tgcgcagtct ggaagcacta gtacgttggc agtcacctga acgtgggttg
    25321 attccaccgt tggacttcat ttcctacgcc gaagagtcag ggctaattgt gcctttaggc
    25381 cgttgggtga ttctcgatgt cgtacgccaa gtggcaaagt ggcgggataa aggcataaac
    25441 ctgcgagtgg cggtaaatat ttctgcacgt cagctcgccg atcaaaccat tttcaccgcc
    25501 ctgaaacagg ttctccagga actcaatttt gaatactgcc ctatagatgt tgaactgaca
    25561 gagagttgtc tgattgagaa tgatgaactg gcactgtctg ttattcaaca atttagccaa
    25621 ctaggtgcgc aagtgcatct ggatgatttt ggtaccggct actcttcact ttcgcaactg
    25681 gcgcgctttc cgatcgatgc catcaaactt gaccaggttt ttgttcgaga tattcacaaa
    25741 caacctgtct cgcagtcact ggtccgggcg atcgtcgctg tggcccaggc attgaatctt
    25801 caggtgatcg ccgaaggtgt agagagtgca aaggaagatg cttttttaac caagaacggg
    25861 atcaatgagc ggcaaggatt tttgtttgcc aaaccgatgc ccgccgtcgc cttcgaacgc
    25921 tggtataaac gctatctgaa gcgcgcataa gtagatgaat atatatactt agcgctatat
    25981 cccgtcatta tatatcgtat tcctggatga taattagccg ttgctgttaa acacttctct
    26041 tttagcggca atttttgcat tattcatttt cccccttttg ctataaggat gtccttgatg
    26101 tctgaatttg atgcccagcg cgttgccgaa cgtattgata ttgtgttaga tatccttgtg
    26161 gcaggcgatt accactctgc gatccataat ctcgagatac ttaaagcaga actcttgcgc
    26221 caggttgctg aatctacgcc agacatcccg aagacaccgt gggagattta acaatcgtgc
    26281 cttcaggttt tcctatttga ggatgcccgg cgcgtcagta tttttttatt tagtattata
    26341 acgttataag aattacagcg atgaattccc gacaacaaac tattctacag atggtcattg
    26401 accagggtca ggttagcgta accgatctgg caaaagccac tggagtttct gaagttacca
    26461 ttcgccagga tctcaacacc ctcgaaaaac tgagttacct ccgccgtgca catggctttg
    26521 cagtttcgct tgatagtgat gacgtcgaaa cccgtatgat gagcaactat acgctgaagc
    26581 gtgaactcgc cgagtttgcc gcgtcactgg ttcaaccggg cgaaaccatc tttatcgaaa
    26641 atggcagcag caatgccctg ctcgcccgga ctctggggga gcagaagaaa aatgtcacta
    26701 tcatcacggt cagcagctac atcgcgcatt tgctgaaaga cgcgccttgt gaagtcattt
    26761 tgctcggtgg cgtgtaccag aaaaaaagcg aaagtatggt tggccctttg acacgccagt
    26821 gcatccagca ggtacatttc agcaaagcat ttattggtat tgatggctgg caacctgaaa
    26881 ctggattcac cggtcgcgat atgatgcgca ccgatgtggt caatgccgtg ctggaaaaag
    26941 agtgcgaagc gatagtcctg actgacagct cgaaatttgg tgctgtacat tcatactcca
    27001 tcggtcccgt tgagcgattc aatcgcgtga ttaccgattc gaaaatacgc gccagcgatc
    27061 tgatgcatct tgagcacagt aaactcaccg ttcacgtcgt tgacatttaa ccatttccct
    27121 tctctacgga tgatttgcag tttggcaaat catccgctct aagatgattc ctggttgata
    27181 attaagacta tttacctgtt attaacactc tcaagatata aaattattat cagcgatata
    27241 acaggaagta attatcacct gcgtgatata accctgcgcg cgagcagatt tcacggaata
    27301 atttcaccag gcttattctt agctattata gttatagaga gcttacttcc gtgaatcata
    27361 aattcaggag agagtattat gtttgtaacg agcaaaaaaa tgaccgcggc tgttctggca
    27421 attactttgg caatgtctct gagtgcctgt tctaactggt ctaaacggga ccgcaacacc
    27481 gcaatcggcg cgggtgcagg ggcattaggc ggtgcagtac tgaccgatgg cagtacgttg
    27541 ggtacattag gtggtgcagc tgtcggtggt gttattggtc accaggtcgg taaataatcg
    27601 taaataaatg tcgattatcg actcgcaatt ataatacgct tgattctgtt cagtacgaaa
    27661 taactgaata atacacacag taaaatattc gaaagccacg gatatatccg tggcttttta
    27721 tgttaaccgc ctgcgagttt taccttcatc cctttcgctt caagtagtga ttttaataaa
    27781 tcacgcttat cgccctggat ttcaataact ccatctttaa ctgctccgcc gcagccgcat
    27841 tttttcttca gttccgctgc aagttttgtc agttcggcat catcgagatc gataccggta
    27901 atcaggcaaa cgcccttacc tttacgtcca ctggtctgac gctgaatacg caccgctcca
    27961 tcgcctttag ggcgtactgg ggccgctttg ggctcatcaa tacgcccggt ttccgttgag
    28021 tagaccagac ggctgttgga atcactcatc atgcactccg ctgtaaagag gcgttgatcg
    28081 ctttcagtgt ctgcgctgga tctaccgatt gcgttaccgg gcgaccaatc accatataat
    28141 caacaccagc cgccaacgcc tgttctggcg tcataatgcg gcgctggtca ccagcttcac
    28201 tcccctgcgg acgaatgccc ggtgtaacca gtttgaactc ctgaccgaat acctgtttaa
    28261 agcgcacagc ttcctgagca gaacacacca caccatcaag gtcacatttt tgcgtcagtg
    28321 ctgccagacg ttctgcataa tctgcaggtg acagtgtcac gccaagatcg gccaggtcgc
    28381 tggcttccat gctggtcaac actgtcacag caatcaaaag cggtgcatct ttgccaaacg
    28441 gaaccagtgc ctcacgcgct gcggtcatca tacgcgcccc accggaggca tgaacattca
    28501 ccatccacac gcctaagtca gctgcagcag cgacagcgtg cgctgcagtg ttggggatat
    28561 cgtggaattt caggtcaaga aagatatcaa aaccacgctg ttgaagttcg cgcacaaact
    28621 gtggcccaaa caatgtaaac atctctttac cgaccttcag acgacaatcg cgtgggtcga
    28681 tcttgtcgac aaaggacagc gcgtcatcac aattatgata atcaagggca acaaccacag
    28741 gagaattcgt aacggcgcgg gaagaagatg aagcagttaa cgtcatgacc agaccttctt
    28801 gatgatgggc accgggagtg gcgcgaaaca ggtaaacggc gagcattcta cccgtgaaaa
    28861 taaaaaatta acagttgcga tttttcccct agcaggcttt ttctgctccg tcgttgccgg
    28921 agcacaatgg gacatcaata actagtatgt tgtaactaaa gtcggatttt tttaaaaatt
    28981 acaggccatc aagaccgcga atcggtttaa tagttgacca ggcacgacaa gacggacaat
    29041 gccagtagag ggtgtatgcg gtaaaaccac atttctggca gctataacga ggcttactac
    29101 gcaccttctc gccaaccatg tcacgcagca ccatcagact ctctttggcg cgcccttctt
    29161 ccgcttcatt taagtggtaa tccattagct tatggaatac acgcatggtc ggatgacgct
    29221 gaagctggcg tgtaatatag acctgtgcag cctcactacc gtcgcgcgct tcgatgatat
    29281 cagcaagcat caattcagca tcggcaccgg tgttctcttc caccgcgcgt tgcaggaatt
    29341 ctgcccattc ggcagtttta cccaactgct gatagcaggt ttgcaacatt tccagcgttt
    29401 cgctgaccag ttctctgtcc tgggatatga cgcgttgcaa actttcgacg gctttggcgt
    29461 attctccttt cgccataaac acgcgtccca tcatgatgga tacgcgggcg ctgtttttat
    29521 ctgccgccgc cccttttttt agcaaggtca tggcacgatc gagatcgtcg ctggccatat
    29581 gctgcagggc taactcacag tagaaatggg caatttcgac gcgctgttta tctttaccca
    29641 gcttcaccag gcgttcggca acatcaattg ctttctgcca ctcactggta gcctggtaga
    29701 tttgtagcaa ctgttgcagc gcgccaatgc ggaagtcagt ttcatcggtc agctgattga
    29761 acatgtcttc cgcgcggtcg tataacccgg cggccatgta atcacgcccc agttgttgaa
    29821 tcgccaacag acgctgttca taggtcagcg aggcgctttc cattagggtc tgatggatgc
    29881 gaatagcgcg atcaacttcg ccacgcgaac ggaacaggtt tccgagcgta aggtgggctt
    29941 caacggtgcc tgtatcctct ttaagcatat cgagaaacag gtctaccgct ttatcctgtt
    30001 gattactaag caggaagtta acccccgcta cgtaatcacg cgacaagcgg ttggcttcat
    30061 cttgcttgtt ttgttgcgca cttctgcggc ccatatacca gccataggca gcggctacag
    30121 gcaaaagcag aaacaacaac tccagcatag aaagttattc cttcgccgct gacgagtgcg
    30181 gcactacagc cacgtcagtc gcgggtgaaa gctggttttc cagtcgcttt attttacgtt
    30241 cagcgcgcgc cagggaaaca cgaactcgca gccagaacag gccacaaatc aaccaaccga
    30301 tagcaaaccc cgcagcaaac aataccgcca gcaatgtgga aatacggtac tccccttgcg
    30361 ctaacagata attaaacgtc acctgttgat cgttctgcgc acccaacgtg accgaaatca
    30421 cgaagatcgc taacaccagt aagaaaatga gtaaatattt cacattactt cccgttatgt
    30481 ggttagagcg aataaattat ggtcaactta ccgcaatagc cctttaaagt accattttcg
    30541 cgccgtctgc gaaatggcat ccacgcgcat tgttgatgat ttaaggccta aattcggaaa
    30601 atcaaccagc gttaactttc ttgttctcgt tgtgcaattt cgcgattttc ttccgcaggt
    30661 ggtgttaatg gcccacaaat tcgttgcgca agccacgttg ccaccgccac cagcgcccac
    30721 gaaatcaacg tagctaccac cagatcgcgt ggccaatgca tcccgagcag caggcggctt
    30781 cccatgactc ccgttgccca gaccaacaag atagcaatgg ttaacgttcg ccgacgcggc
    30841 cacagcaaac caacggccag cagtgcccaa ctggcagcaa acatcgtgtg accggaagga
    30901 aaggcaaacc ccgtctcttt ctgccagtgt gaacgcaaat attgtggaat atttttctct
    30961 tcagccaact gttcttttac tagatttccg cgttctgctc gctttaaagt gtagaactca
    31021 tcaaccggaa tatgatgtgt tttttccaac cagataacaa aaggtcgtgg ttcctgtact
    31081 ttgtctttga tccaggattt aacgccttgt ccaacaagga ttgccgccgc cagaatggca
    31141 aataatacaa aggcagcctt aatgcgaaaa cgcagacacc agagaaacca gccgaacaaa
    31201 atcaaatgtg taatgacgcc ccagggctgg gtgacagttt cagtaaccca aaaagccgct
    31261 tttagtagcc aactttgttc tccaggttgc caacgccagc cagaaatcca tacggctact
    31321 ggcatgacaa gcaatagtgc agctcccact gcggtacgtc gggcaatcga acgcatggcc
    31381 tctccttttt gataagtccc acaatcataa ctgaaaatgc cagttccaag aaaaattgac
    31441 agatttgtgc cattccgtga acgatcgacg cgtcgtgatt aggtgaaccc cttctcgtta
    31501 tggcaaaata agccaataca gaaccagcat tatctggaga atttcatgca gcttaaacgt
    31561 gtggcagaag ccaaactgcc aaccccatgg ggcgatttcc tgatggtggg atttgaagaa
    31621 ctggcaaccg gacacgatca tgttgcgcta gtctatggcg atatttccgg gcataccccg
    31681 gtacttgcgc gcgtccattc cgaatgtctg accggtgacg ccctgttcag cttgcgctgc
    31741 gattgtggct tccagctcga agcggcattg acgcaaattg ccgaggaagg ccgtggtatt
    31801 ttgctgtatc accgtcagga aggtcgtaac attggtctgc tgaataaaat ccgcgcttac
    31861 gcactgcagg atcaaggtta cgataccgta gaggctaacc accagttagg cttcgccgct
    31921 gatgagcgcg acttcactct ttgcgctgat atgttcaaac tccttggcgt caatgaagtc
    31981 cgcttgttaa ccaataaccc gaaaaaagtc gaaattctga ccgaagcagg gattaatatt
    32041 gttgaacgcg taccattgat tgtaggtcgt aaccccaata acgaacatta tctcgatacc
    32101 aaagccgaga aaatgggcca tttgctgaac aaataaccct cttgcatcgt gtaattcatt
    32161 tgcttgccgg aagcaaaata accggcaagc aaatacttgt tacttcaaca tattacgaat
    32221 gacataatgc aaaatgccgt cgttctggta gtaggtcaac tccgtcgcgg tgtcgatacg
    32281 acaacggcag ggtacgactt cctggctacc atccgcgcgc gtaagcgtca ccggaaccgt
    32341 cgcgccaggt tgtagatttt gcagatcgcc aatatcaatc ttctcttccc cggttagccc
    32401 taacgtttta cgcgtcacgc cttgcggaaa ttccagcggc aggatgccca tgccaattaa
    32461 attcgaacgg tgaattcgtt caaacgattc ggcaatcacc acacgaattc caagcagacg
    32521 aggtcctttt gccgcccagt cacggcttga gcctgatcca tactctttcc cggcaatcac
    32581 cgccagcggc gtttgctcct gcttatagcg catcgcagca tcataaatag agactacgtc
    32641 gctgtcaggt aaatgccgcg tcatcccccc ttcaacgcca ggcaccattt cattacggat
    32701 gcgaatattg gcgaaggtgc cgcgcatcat cacttcatgg ttaccacgcc gcgaaccgta
    32761 ggagttaaag tcttttcgct caacaccccg accttgtaga tatcgacccg ctgggctgtc
    32821 gggcttaata ctgcccgccg gagagatatg gtcagtggtg actgaatccc ccagcattgc
    32881 gaggatccgc gcaccgtgaa tatcttccac tggtgctggt gttgcctgca tttcatcaaa
    32941 gaaaggcgat aagcgaatat aggttgagtc ctcctgccaa ccgtaggtat cggatcgtgt
    33001 gacgttaatt tccttccact ctgctgtgcc ttcaaaaact tctgcgtact ctttgcggaa
    33061 catttctgtg gagacttgtt ctaccgcacg ggcaatttct tgtgccgatg gccagatatc
    33121 tttcagataa accggctcgc ctttgcgatc atggccgata ggctcagaag ccaggttgat
    33181 attcatattt cccgccagcg cataggcaac caccagcggt ggcgaggcca gccagttagt
    33241 tttaaccagc ggatggatac ggccttcaaa gttacggttg ccggacagca ccgcaccgac
    33301 ggttaaatcg ccttttttga ttgccgtttc gataggatcg ggcagcggcc cagagttacc
    33361 aatacaggtg gtacaaccgt atcccacaag gttaaacccc agttcgtcga gatacggtgt
    33421 cagtttcgct tttgccagat aatcagaaac gactttcgaa cccggtgcca acgacgcttt
    33481 gacccacggt tgccgcttga ggccaagagt tacggctttt ttcgccagca agcctgcggc
    33541 catcagcaca cttgggttag aggtgttggt gcacgaggtt atcgcagcaa tgaccacagc
    33601 gccatcaggt aactgatact gatgtccgtt cataacataa tcgactggca ggcgatcttt
    33661 atgcgtggca ttcactttca gttcgttact ggcggcaaat gcttttggta catcgggcag
    33721 tgcaacgcga tcctgtgggc gttttggccc tgccaggctc gcttcaacgt cattcatatc
    33781 cagttctaac atactggtaa aaatcggttc atcgcccggg ttacgccaca tgccctgcgc
    33841 tttggcatat ttttcgacca actcgacctg atcttcgctg cgcccgctta aacgcatgta
    33901 atcgagggtt actgcatcga ttgggaagaa gccacaggtg gcaccatatt ctggcgacat
    33961 attggcaatg gtggcgcgat ccgccaacgg tagtgaatcc agaccatcac cataaaattc
    34021 gacgaatttc cccaccacgc catgtttgcg cagcatttgg gtaacagtga gaaccaggtc
    34081 tgtggcggta ataccttcac gtaattttcc ggtaagttta aagcccacta cgtccgggat
    34141 aagcatggaa accggctggc ctaacatggc ggcttctgct tcgatcccac ccacgcccca
    34201 ccccagcacg ccaaggccgt tgatcatagt ggtgtgcgag tcagtaccaa cgagtgtatc
    34261 cggataagca atccattcac cgtcctgcaa ttcgctccac actgctttgc cgagatattc
    34321 gaggttaacc tgatggcaaa tgcctgtgcc tggcggcacg acgctaaacc gactgaacgc
    34381 ttgctttccc catttcagga acacataacg ttcgtggttg cgctccattt ccaggcgtac
    34441 gttttcttca aatgcctcat catcaccaaa acgatcgacg gtcaccgagt ggtcaatgac
    34501 caggtcgacc ggtgagagcg ggttaacctt tgcagtatcg ccaccgaggc gtttaaccgc
    34561 ttcgcgcatt gccgccagat caacaacggc aggtacgccg gtaaagtcct gcatcagcac
    34621 ccttgccggg cggtaggcaa tttcacggtc agcatgggca tttttcagcc atcccgccag
    34681 tgcgtggata tcctcttcgg taactgagtt accatcctgc cagcgcagca ggttttcgag
    34741 caaaactttg agtgacttgg gtagacgggt gatatcgccc agtgatttag cagcaagcgg
    34801 caggctgtag tagtggtaag ttttatcttt ggcctgcaac gtgtccttac tggcttctcg
    34861 tagggttgac gacatagctc ctccttaatg acagggttgc gaagccctga ttctcttcag
    34921 ggtgaatatt aaagataaca caaacatcgc gtaacgattt gataacaacc caaattgata
    34981 aaagaggaga aaccttgtgg caaagcaaat gacaaccccg ccgcagcggg gtcaaggatc
    35041 agtgagtaat gatccagagg agttcggccc agaagagcat cgacagagaa aacgcgccca
    35101 tccaggacca atatttcata tttgtgttga tcataggcta cacccttaac ggttattatt
    35161 ttgtgttgag ataactcaag ctgttattgc tgcggtgtat taaaagccct ttaattcatt
    35221 aaaaatgggt aataagaatg atgataatta ttgtttcggc tgatcatctt cagcaaacag
    35281 ttctataaag gcttgctgct gcgatgttag ttgccagtcg tctggaataa cgacggaaca
    35341 ttcccgtttc gtttctggtt cattgtcact atgctggcat aatggtgacg actccagttg
    35401 ctcctgaccg accatagtta tccccacact ttccaaatca gcagtgcgac aaccacccag
    35461 aaaagtgcag aaccaagaaa aaccgccagc caggctttac gttttaattc aggatcacgg
    35521 cggggttcct gatttccaga aggcattgct aacctcatat aatcgccatc acttatcagc
    35581 aagacgttga atgattggtg taattaatca tacgatgaga caaatcctaa agaaacttta
    35641 gtcaaaaatc cagcgaattt acgaatcgtc tccagtgaat aattcaatct tttgacctga
    35701 aatagatatt tgaaaactgg aatttgcgag gcgggtaatt agacactact ttacaggtaa
    35761 tttaccctat aaatatgagg gataaaaata ttgtgcacgg tatagaaata agtaacaaaa
    35821 gagaatatat tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat
    35881 ttttccggca gttttatatc tttaaacatg acctcaattt cttcattgga gcgcaatgcg
    35941 acagccgcat caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac
    36001 atataactgc gcaaaaaagt actacggcga aaaccaattt tggttgtact gtggctgaag
    36061 atatcgtgag catcaacgcg cacaaggtcg ggatcggcga ccggatccac cgccatgctg
    36121 gcaatgaccc ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt
    36181 gccgtgaaaa cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa
    36241 cgtccggtaa agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta
    36301 atggcttttt tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg
    36361 tagcacggta acatcactaa atcttcatac agatgcagcg cttctgtggc gatagcgaaa
    36421 tcggcattgc ctttagagac ggcatcagca atttgtgtcg gcgggccctg gtgcatatgc
    36481 aaagaaacgc ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt
    36541 gcctgggtat gcgtggtggc gatatacagc gagcctttat ccggccaggt gtgctcaccg
    36601 gcaaccgatt ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct
    36661 tgtcctgctg gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct
    36721 agctcgtctt ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct
    36781 tccgctgttg atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt
    36841 tgtaatttca tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac
    36901 ctggcgggtg actaatacga tttaataata aaaaaggtgc taactataac cactatatca
    36961 tttatagcct tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt
    37021 tcgcgaccct ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc
    37081 atttgccatc aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga
    37141 catattgctg tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct
    37201 gctgtggcgc atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa
    37261 gctcttccac cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga
    37321 acacaccggc agcaccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct
    37381 caggtaatgg caccggatct tctttcggtg gtgccacttc accgttacgt aaaatcttac
    37441 gtgtgttttt acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca
    37501 ggtgcatttc agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc
    37561 ggaattcgcc ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca
    37621 acttacgttt cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac
    37681 gttttgcgcg cagcgcgttg gtttcagcat cttcgccttc cagcacgttc agcacttcgt
    37741 tttccggcac caggttaatg gtggttttgc aacgctcttt cggcggcagc gcatagccag
    37801 aacagccaag gaataccccg gtactcgctg tgcgaatccc cattttgcga ccacaagtcg
    37861 ggcagtcaat gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt
    37921 tttcagcttt atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc
    37981 actctgcttc gtgatttgcc acctggtcga ggctgttttc catctgcgcg gtaaagtcgt
    38041 agttcattaa ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg cccatttttt
    38101 ccgcatagaa acgacgattt tctactcgca catagccacg atcctgaatg gtcgaaatga
    38161 tcgacgcata ggtagacgga cgaccgatac cacgtttttc cagttcttta accagcgatg
    38221 cttcactgaa acgggctggc ggcttggtaa agtgctgggc tggtgttagt tcaacgagcg
    38281 tcagagcatc gcctttatcg actgccggta agatacgatc ttcatcgcct ttacgcagtg
    38341 caggcatcac ttttgtccag ccatcaaagc gcaaaatacg accgcgtgct ttcaggcgga
    38401 aatcgcccgc accaacggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg
    38461 caacgaactg acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca
    38521 gcgattccgc catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt
    38581 tttctttgct ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc
    38641 tgatataacc gcgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca
    38701 tgtaagtgat atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc
    38761 caaaaccaag acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag
    38821 gtttactggt tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca
    38881 gacttaccgc agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcattctgat
    38941 gagtcacctg caacgccaac gcttcaccag atggcgtagt cgtgctggca tcgacttccc
    39001 agaactcttc cggtacgaac gctttaattt cacgctcacg ctctaccacc aggcggaccg
    39061 ccaccgactg cacacgaccg gcagacagac cacgagcgat ctttttccac agcagcggcg
    39121 aaaccatata ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat
    39181 caatattcag ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt
    39241 taaacaccac tcggctatag cgcgcatcat caccaccaat cacttcccgc aggtgccatg
    39301 caatggcttc cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag
    39361 ccagttgttt cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct
    39421 cccaattgtg ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt
    39481 taggcttttt agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac
    39541 tggtcggcaa atcgcggatg tgaccgacgc tggatttcac cacgtagtca caacccagat
    39601 acttgttgat cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat
    39661 tcaccttacc taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg
    39721 caacgagtta ccgatatgga ctcgctctct gcggatatca accccttgtc gtgactctgt
    39781 tcacgaaaat tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg
    39841 gaattttggt cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac
    39901 ctgataaaat ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg
    39961 ctcttgccac cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct
    40021 tgtttaagga gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga
    40081 aagaggccaa caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg
    40141 gcgtcacgca gcgtaatggc gtgctggtat ttaccggcga ctacttttta gatgaacaag
    40201 gattgccaac agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc
    40261 tttcagaaaa atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg
    40321 tcttttacat caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc
    40381 tctctgccgc gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat
    40441 taaccacttc acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat
    40501 caaccaggcc tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt
    40561 caatatccag agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt
    40621 tcagctcttc gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg
    40681 tgcgcttata ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc
    40741 ggttaaagtt gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg
    40801 aaacaatttt gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta
    40861 ccgtaacagt taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc
    40921 cgtaaccatg caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt
    40981 tgaatgctgc gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca
    41041 tgctgccttt aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca
    41101 gtttggcttt cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt
    41161 gccactgttt ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct
    41221 tatactgttc gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag
    41281 caacattgac aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttag
    41341 ccaaaaacaa accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc
    41401 tttggctcac agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga
    41461 aagtatgaca aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc
    41521 tgtttcaggc ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg
    41581 ttgaggagta gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct
    41641 ggtgacggga gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg
    41701 tgcgacagtg attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat
    41761 aaacgaagaa actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc
    41821 cgaaaattgc caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt
    41881 tttgcataat gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt
    41941 ctggcaggac gtcatgcaga tcaacgttaa tgccaccttt atgctcaccc aggcactgct
    42001 tcctttatta ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttggacg
    42061 tcagggacga gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat
    42121 gcaggtactg gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg
    42181 tacgcgcacc gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac
    42241 acccgctgat atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac
    42301 cggcatgacc tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa
    42361 cgctaccaac agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc
    42421 caggatgaac gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg
    42481 gcatttggta cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt
    42541 attaaaggca cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt
    42601 caggtgatgg caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc
    42661 tgccgcgaag tctggcaaca tgcaaagcgg atgcttgctg attcctcact ggatatggtt
    42721 ttgcttgatg aactgacgta tatggtggcg tatgactatt tgccactgga agaagtggtg
    42781 caggcgttaa atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat
    42841 cgggatattc ttgaactggc ggatacggta agtgagctgc gccccatcag acatgcgttt
    42901 gatgccggtg ttaaggcgca aatagggatt gattattaat ctgtaaaaat gccggatatg
    42961 acgtccggca tactttgatt aaccgttatt acgcccgcca gaacgacgac tgccgctcac
    43021 ctgactgtga cgtttcaccg cacgacgaat ctgattagcc ttcatgcgac gacggtcttt
    43081 ttctacagcg actttagagc tggtttctgg cggaagctcc accagttcgc gcagatagtt
    43141 agtctgggcg aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat
    43201 atcaccgtaa cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg
    43261 acgaacttca cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc
    43321 gccgccgctg aacttgatgg ttttaaaagc tgccggacca tcttccagtt gcacgccacg
    43381 actcaaatca cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg cgtattcacg
    43441 ttcaacttca cggcttggat gcattaaacg gtttgccagt tcaccatcgg tggtgaacag
    43501 cagcagacca caggtgttaa cgtccagacg acctacggca atccagcgtg cgccacgcag
    43561 ctttggcaga cggtcaaaca ccgtcggacg cccttccgga tcgttacggg tacacaactc
    43621 accttccggt ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga
    43681 aatcagatga ccatcgatac gaattttcag cccaggggta acttcaacgc gatcgccgag
    43741 tttagcaatt ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg
    43801 gcgggagccg tggccagcgc gggccagcac tttctgtaat ttttcgctca tagagcttcc
    43861 ttttggtgtc gccttcacag gcgtcgaaca ggagaatcaa tggcgccgta tagcgccatt
    43921 ttaaggccgc gtagtataca gagttacgca cttataagaa aggcttcaca tcacctacgc
    43981 cttcacgcac cacgaccggc gtatcatcgg taagatcaat aaccgttgtt ggtttctgcc
    44041 cgagatagcc gccgtgaata atcaaatcta cctgtttttc cagacggtct ttaatttctt
    44101 cgggatcgga ttcggtaaat tcgctgcctg gcagcattag cgaagtggaa agcatcggtt
    44161 cgcccagtgc ttcaagcaac gcctgcgcaa tcgggttaga aggcacacgc ataccgatgg
    44221 ttttgcgttt ctcctgcaac agacggcgtg gcacttcctt cgtccccttc aggatgaagg
    44281 tatagttgcc cggcgtgttg tttttcatca aacgaaacgc aacgttatcc acgaatgaat
    44341 aggtcgagag ttcagaaaga tcgcgacaca tcagggtaaa gttgtgaccg tccggcagct
    44401 ggcgaatacg acaaatacgc tccatggcgt ttttatcttc aattttacag ccgagcgcat
    44461 agccggaatc agttggatag acaatcaccc cgcctttacg cacgatctcc accgcctggt
    44521 tgatcagacg ttgctgcggg ttatcaggat gaatataaaa aaactggctc ataattccct
    44581 ctctgtggtg ttctgcggct gttcccatag ctgccagacg ccttcaacgc ctgcgggcag
    44641 ccagagttta cgacccagtt cgatccacgg gcatggctga tgaaaatcag atccttgtga
    44701 tgcccataaa tgatgctgac gcgcaagtgt ggccagctgg gtgcgttcat tgggcgattg
    44761 ctggcactgc gcgacttcca tcgcgtcgcc gtggtgttcg gcaaaatgcg caaccaatct
    44821 tttcagccat ttagcactaa gattgtaccg cccaggatga gctagcaccg ccttaccgcc
    44881 agaatgatga atgacatcaa tagcttgttc tattgtacac cactgtggcg gaacgtatcc
    44941 ggttttcccg cgcgccagat actttttaaa gacatctgcc attgaactgg ctttgccgca
    45001 ctccacaagg aaacgcgcaa aatgaccgcg cgtcactgcg ccgccctgcg ccagtcgttg
    45061 tgcgccttcc agcgccccag gtatttgcgc tttttcaagt ctttcggcaa tttgctgcgc
    45121 ccgctgattg cgccgttctg tctgctgcgc gaggaactca cacattagcg gatgagtaat
    45181 atcaatattc agcccgacaa tatgaatttc atgattttcc cagaccgtgg aaatttccac
    45241 gccgggaata agattcagcg ccagtcccga acgtgaaatt tcctctcttg ctggcgcgat
    45301 tgcagctgtg gtgtcatggt cggtgatcgc cagggtgccg acgcgcatct cgactgcacg
    45361 gtgcaccaat gcttctggcg tcaggcagcc atcggaagct gtggtatggc tgtgcaggtc
    45421 gtaaatcact gcataattcg tgtcgctcaa ggcgcactcc cgttctggat aatgtttttt
    45481 gcgccgacat cataacgggt ctggcaaata ttctgaaatg agctgttgac aattaatcat
    45541 cgaaccagtt aactagtacg caagttcacg taaaaagggt atcgacaatg aaagcaattt
    45601 tcgtactgaa aggttggtgg cgcacttcct gaaacgggca gtgtattcac catgcgtaaa
    45661 gcaatcagat acccagcccg cctaatgagc gggctttttt ttgaacaaaa ttagagaata
    45721 acaatgcaaa cacaaaaacc gactctcgaa ctgctaacct gcgaaggcgc ttatcgcgac
    45781 aacccgactg cgctttttca ccagttgtgt gggaatcgtc cggcaacgct gctgctggaa
    45841 tccgcagata tcgacagcaa agatgattta aaaagcctgc tgctgataga cagtgcgctg
    45901 cgcattacag cattaggtga caccgtcaca attcaggcac tttccggcaa tggcgaagcc
    45961 ctgctggcac tactggataa cgccctgcct gcgggtgtgg aaagtgaaca atcaccaaac
    46021 tgccgtgtgc tgcgcttccc ccctgtcagt ccactgctgg atgaagacgc ccgcttatgc
    46081 tccctttcgg tttttgacgc tttccgttta ttgcagaatc tgttgaatgt accgaaggaa
    46141 gaacgagaag caatgttctt cggcggcctg ttctcttatg accttgtggc gggatttgaa
    46201 gatttaccgc aactgtcagc ggaaaataac tgccctgatt tctgttttta tctcgctgaa
    46261 acgctgatgg tgattgacca tcagaaaaaa agcacccgta ttcaggccag cctgtttgct
    46321 ccgaatgaag aagaaaaaca acgtctcact gctcgcctga acgaactacg tcagcaactg
    46381 accgaagccg cgccgccgct gccagtggtt tccgtgccgc atatgcgttg tgaatgtaat
    46441 cagagcgatg aagagttcgg tggcgtagtg cgtttgttgc aaaaagcgat tcgcgctgga
    46501 gaaattttcc aggtggtgcc atctcgccgt ttctctctgc cctgcccgtc accgctggcg
    46561 gcctattacg tgctgaaaaa gagtaatccc agcccgtaca tgttttttat gcaggataat
    46621 gatttcaccc tatttggcgc gtcgccggaa agctcgctca agtatgatgc caccagccgc
    46681 cagattgaga tctacccgat tgccggaaca cgcccacgcg gtcgtcgcgc cgatggttca
    46741 ctggacagag atctcgacag ccgtattgaa ctggaaatgc gtactgacca taaagagctg
    46801 tctgaacatc tgatgctggt tgatctcgcc cgtaatgatc tggcacgcat ttgcacccct
    46861 ggcagccgct acatcgccga tctcaccaaa gttgaccgtt attcctatgt gatgcacctc
    46921 gtctctcgcg tagtcggcga actgcgtcac gatcttgacg ccctgcacgc ttatcgcgcc
    46981 tgtatgaata tggggacgtt aagcggtgcg ccgaaagtac gcgctatgca gttaattgcc
    47041 gaggcggaag gtcgtcgccg cggcagctac ggcggcgcgg taggttattt caccgcgcat
    47101 ggcgatctcg acacctgcat tgtgatccgc tcggcgctgg tggaaaacgg tatcgccacc
    47161 gtgcaagcgg gtgctggtgt agtccttgat tctgttccgc agtcggaagc cgacgaaacc
    47221 cgtaacaaag cccgcgctgt actgcgcgct attgccaccg cgcatcatgc acaggagact
    47281 ttctgatggc tgacattctg ctgctcgata atatcgactc ttttacgtac aacctggcag
    47341 atcagttgcg cagcaatggt cataacgtgg tgatttaccg caaccatatt ccggcgcaga
    47401 ccttaattga acgcctggcg acgatgagca atccggtact gatgctttct cctggccccg
    47461 gtgtgccgag cgaagccggt tgtatgccgg aactcctcac ccgcttgcgt ggcaagctgc
    47521 ccattattgg catttgcctc ggacatcagg caattgtcga agcttacggg ggctatgtcg
    47581 gtcaggcggg cgaaattctt cacggtaaag cgtcgagcat tgaacatgac ggtcaggcga
    47641 tgtttgccgg attaacaaac ccgctgccgg tggcgcgtta tcactcgctg gttggcagta
    47701 acattccggc cggtttaacc atcaacgccc attttaatgg catggtgatg gcagtacgtc
    47761 acgatgcgga tcgcgtttgt ggattccagt tccatccgga atccattctc accacccagg
    47821 gcgctcgcct gctggaacaa acgctggcct gggcgcagca gaaactagag ccagccaaca
    47881 cgctgcaacc gattctggaa aaactgtatc aggcgcagac gcttagccaa caagaaagcc
    47941 accagctgtt ttcagcggtg gtgcgtggcg agctgaagcc ggaacaactg gcggcggcgc
    48001 tggtgagcat gaaaattcgc ggtgagcacc cgaacgagat cgccggggca gcaaccgcgc
    48061 tactggaaaa cgcagcgccg ttcccgcgcc cggattatct gtttgctgat atcgtcggta
    48121 ctggcggtga cggcagcaac agtatcaata tttctaccgc cagtgcgttt gtcgccgcgg
    48181 cctgtgggct gaaagtggcg aaacacggta accgcagcgt ctccagtaaa tccggctcgt
    48241 cggatctgct ggcggcattc ggtattaatc ttgatatgaa cgccaataaa tcgcgccagg
    48301 cgctggacga gttaggcgta tgtttcctct ttgcgccgaa atatcacacc ggattccgcc
    48361 acgcgatgcc ggttcgccag caactgaaaa cccgcaccct gttcaatgtg ctggggccat
    48421 tgattaaccc ggcgcatccg ccgctggcgt taattggtgt ttatagtccg gaactggtgc
    48481 tgccgattgc cgaaaccttg cgcgtgctgg ggtatcaacg cgcggcggtg gtgcacagcg
    48541 gcgggatgga tgaagtttca ttacacgcgc cgacaatcgt tgccgagctg catgacggca
    48601 aaattaagag ctatcaattg accgctgaag attttggcct gactccctac caccaggagc
    48661 aactggcagg cggaacacct gaagaaaacc gtgacatttt aacacgcttg ttacaaggta
    48721 aaggcgacgc cgcccatgaa gcagccgtcg ctgcgaacgt cgccatgtta atgcgcctgc
    48781 atggccatga agatctgcaa gccaatgcgc aaaccgttct tgaggtactg cgcagtggtt
    48841 ccgcttacga cagagttacc gcgctggcgg cacgagggta aatgatgcaa accgttttag
    48901 cgaaaatcgt cgcagacaag gcgatttggg tagaaacccg caaacagcag caaccgctgg
    48961 ccagttttca gaatgaggtt cagccgagca cacgacattt ttatgatgcg ctacagggtg
    49021 cacgcacggc gtttattctg gagtgtaaaa aagcgtcgcc gtcaaaaggc gtgatccgtg
    49081 atgatttcga tccggcacgc attgccgcca tttataaaca ttacgcttcg gcaatttcgg
    49141 tgctgactga tgagaaatat tttcagggga gctttgattt cctcctcatc gtcagccaaa
    49201 tcgccccgca gccgatttta tgtaaagact tcattatcga cccttaccag atctatctgg
    49261 cgcgctatta ccaggccgat gcctgcttat taatgctttc agtactggat gacgaccaat
    49321 atcgccagct tgcagccgtc gctcacagtc tggagatggg ggtgctgacc gaagtcagta
    49381 atgaagagga actggagcgc gccattgcat taggggcaaa ggtcgttggc atcaacaacc
    49441 gcgatctgcg tgatttgtcg attgatctca accgtacccg cgagcttgcg ccgaaactgg
    49501 ggcacaacgt aacggtaatc agcgaatccg gcatcaatac ttacgctcag gtgcgcgagt
    49561 taagccactt cgctaacggt tttctgattg gttcggcgtt gatggcccat gacgatttgc
    49621 acgccgccgt gcgccgggtg ttgctgggtg agaataaagt atgtggcctg acgcgtgggc
    49681 aagatgctaa agcagcttat gacgcgggcg cgatttacgg tgggttgatt tttgttgcga
    49741 catcaccgcg ttgcgtcaac gttgaacagg cgcaggaagt gatggctgcg gcaccgttgc
    49801 agtatgttgg cgtgttccgc aatcacgata ttgccgatgt ggtggacaaa gctaaggtgt
    49861 tatcgctggc ggcagtgcaa ctgcatggca atgaagatca gctgtatatc gatacgctgc
    49921 gtgaagctct gccagcacat gttgccatct ggaaagcatt aagcgtcggt gaaaccctgc
    49981 ccgcccgcga gtttcagcac gttgataaat atgttttaga caacggccag ggtggaagcg
    50041 ggcaacgttt tgactggtca ctattaaatg gtcaatcgct tggcaacgtt ctgctggcgg
    50101 ggggcttagg cgcagataac tgcgtggaag cggcacaaac cggctgcgcc ggacttgatt
    50161 ttaattctgc tgtagagtcg caaccgggca tcaaagacgc acgtcttttg gcctcggttt
    50221 tccagacgct gcgcgcatat taaggaaagg aacaatgaca acattactta acccctattt
    50281 tggtgagttt ggcggcatgt acgtgccaca aatcctgatg cctgctctgc gccagctgga
    50341 agaagctttt gtcagcgcgc aaaaagatcc tgaatttcaa gcccagttca acgacctgct
    50401 gaaaaactat gccgggcgtc caaccgcact gaccaaatgc cagaacatta cagccgggac
    50461 gaacaccacg ctgtatctca agcgtgaaga tttgctgcac ggcggcgcac ataaaaccaa
    50521 ccaggtgctg gggcaggcgt tgctggcgaa gcggatgggt aaaaccgaaa tcatcgccga
    50581 aaccggggcc ggtcagcatg gcgtggcgtc ggcccttgcc agcgccctgc tcggcctgaa
    50641 atgccgtatt tatatgggtg ccaaagacgt tgaacgccag tcgcctaacg tttttcgtat
    50701 gcgcttaatg ggtgcggaag tgatcccggt acatagcggt tccgccacgc tgaaagatgc
    50761 ctgtaatgag gcgctacgcg actggtccgg cagttatgaa accgcgcact atatgctggg
    50821 caccgcggct ggcccgcatc cttatccgac cattgtccgt gagtttcagc ggatgattgg
    50881 cgaagaaacc aaagcgcaga ttctggaaag agaaggtcgc ctgccggatg ccgttatcgc
    50941 ctgtgttggc ggcggttcga atgccatcgg catgtttgct gatttcatca atgaaaccaa
    51001 cgtcggcctg attggtgtgg agcctggtgg tcacggtatc gaaactggcg agcacggcgc
    51061 accgctaaaa catggtcgcg tgggcattta tttcggtatg aaagcgccga tgatgcaaac
    51121 cgaagacggg cagattgaag aatcttactc catctccgcc ggactggatt tcccgtctgt
    51181 cggtccacaa cacgcgtatc ttaacagcac tggacgcgct gattacgtgt ctattaccga
    51241 tgatgaagcc cttgaagcct tcaaaacgct gtgcctgcac gaagggatca tcccggcgct
    51301 ggaatcctcc cacgccctgg cccatgcgct gaaaatgatg cgcgaaaacc cggataaaga
    51361 gcagctactg gtggttaacc tttccggtcg cggcgataaa gacatcttca ccgttcacga
    51421 tattttgaaa gcacgagggg aaatctgatg gaacgctacg aatctctgtt tgcccagttg
    51481 aaggagcgca aagaaggcgc attcgttcct ttcgtcacgc tcggtgatcc gggcattgag
    51541 cagtcattga aaattatcga tacgctaatt gaagccggtg ctgacgcgct ggagttaggt
    51601 atccccttct ccgacccact ggcggatggc ccgaccattc aaaacgccac tctgcgcgcc
    51661 tttgcggcag gtgtgactcc ggcacaatgt tttgaaatgc tggcactgat tcgccagaaa
    51721 cacccgacca ttcccattgg cctgttgatg tatgccaatc tggtgtttaa caaaggcatt
    51781 gatgagtttt atgcccagtg cgagaaagtc ggcgtcgatt cggtgctggt tgccgatgtg
    51841 ccagttgaag agtccgcgcc cttccgccag gccgcgttgc gccataatgt cgcacctatc
    51901 ttcatctgcc cgccaaatgc cgatgacgac ctgctgcgcc agatagcctc ttacggtcgt
    51961 ggttacacat atttgctgtc acgagcgggc gtgaccggcg cagaaaaccg cgccgcgtta
    52021 cccctcaatc atctggttgc gaagctgaaa gagtacaacg ctgcacctcc attgcagggg
    52081 tttggtattt ccgccccgga tcaggtaaaa gcagcgattg atgcaggagc tgcgggcgcg
    52141 atttctggtt cggccattgt taaaatcatc gagcaacata ttaatgagcc agagaaaatg
    52201 ctggcggcac tgaaagtttt tgtacaaccg atgaaagcgg cgacgcgcag ttaatcccac
    52261 agccgccagt tccgctggcg gcattttaac tttctttaat gaagccggaa aaatcctaaa
    52321 ttcatttaat atttatcttt ttaccgtttc gcttaccccg gtcgaacgtc aacttacgtc
    52381 atttttccgc ccaacagtaa tataatcaaa caaattaatc ccgcaacata acaccagtaa
    52441 aatcaataat tttctctaag tcacatattc ctcaggtaat tgttaatata tccagaatgt
    52501 tcctcaaaat atatttccct ctatcttctc gttgcgctta atttgactaa ttctcattag
    52561 tgactaattt taatgagtgt cgacacacaa cactcatatt aatgaaacaa tgcaacgcaa
    52621 cgggagaaat aacatggccg aacatcgtgg tagttcagga aatttcgccg aagaccgtga
    52681 gaaggcatcc gacgcaggcc gtaaaggcgg tcagcatagc ggcggtaatt ttaaaaatga
    52741 tccgcaacgc gcatctgaag cgggtaaaaa aggcggtcaa caaagcggtg gtaataaatc
    52801 aggcaaatcc tgattcaccg ttaattatta atatcagctg ataaagataa ttgcatctgc
    52861 gggccgtatg gctcgcagtg atttcactgg agaaaaatat gaatatgaag accattgaag
    52921 atgtatttat tcaactgctt tcagatacct acagcgcaga aaaacaatta acccgggcac
    52981 tggcaaaact cgcaagagca acatcaaatg aaaaattaag tcaggctttt catgcgcacc
    53041 tcgaggaaac tcatggacag attgaacgaa ttgatcaagt tgtggaatca gaatcaaatc
    53101 tgaaaattaa gcgcatgaaa tgtgtggcaa tggaaggtct tattgaagaa gctaatgagg
    53161 tcatcgaaag taccgagaaa aacgaagtgc gtgatgccgc actgattgcc gcagcacaga
    53221 aagtcgagca ttatgagatt gccagttacg ggacattagc gacgctggct gaacaattag
    53281 gttaccgtaa agcagcgaag cttctgaaag aaaccctgga agaagaaaag gccaccgaca
    53341 tcaaactgac tgatctggcc cttaataacg taaataagaa agccgaaaat aaagcctgaa
    53401 atatgaattt taacttttag tcattttata aagaggacat tttcatgaat cgtattgaac
    53461 attatcatga ctggttacgt gacgcccacg caatggaaaa gcaagccgaa tctatgcttg
    53521 aatccatggc cagccgtata gataattatc ctgaactacg cgctcgtatt gaacaacatc
    53581 ttagtgaaac caaaaaccag attgttcaac tggaaactat tcttgatcgt aatgacattt
    53641 cacgttcagt cattaaagat tccatgagta aaatggctgc gcttgggcag tcaatcggtg
    53701 gtatattccc ttctgatgaa atagttaaag gctctattgg cggatatgtc ttcgagcaat
    53761 ttgaaatcac ctgttacacc tcactattag cagcagcaaa aaatgccggt gatacagtct
    53821 caattccgat catcgaagcg attttaaatg aggaaaagca aatggccgac tggctgattc
    53881 agcatattcc gcaaacaact gagaaatttt taattcgttc tgaaactgat ggcgtagaag
    53941 cgaagaaata atacccttta taccatgtcc ttattgaccc cgtatattac ggggtcgttt
    54001 ttgtgcggaa ttaaaaacga tatcctgctg agaacataaa cacccacgga tcgaggcgta
    54061 cgctatcgtg ttgctgtgca ccgcctagct tataattggc ggtggtatcg atatccatgt
    54121 accacactga catgttaacc aaccagtcac ggttaatcag ataatcaacc cccacctgcc
    54181 cggcagctcc ccaggaatct ttcagactga gatcggaaag ccccgcctct ttgccatgat
    54241 cgttaaatcc attatcaaag aaggtggtgt agttaatacc ggccccaacg taaggacgga
    54301 atttgctgct ggcatcacca aaataccact gcgccatcag tgttggcggc agatggtgaa
    54361 cggttgcaat atcgccggtc gcccgggtgc cgattttatg gcggaacggc gtcgctgcca
    54421 gtaattccac accaatgttg tcggtcgcca tataagtaaa cgtcaagccc agttgagtgt
    54481 tattggtcac gctgaatcca cccagacttc ctaacgtacc accagcacct tctgttggac
    54541 gtacggttgc agaacctgca cgcataaaaa attcgcctgc ttcatgcgca aaggcactgc
    54601 cagaaagaag agttgttact gccaaagccg ccactgttaa ctttttcata tccgctccgt
    54661 cgttatggtt ataaaaacgt gacgaatata cctacattgg agtaacaaat gatcctacat
    54721 agatcacatt acatatgata attcaccatt tattgatctg gattaattta gaagttgcat
    54781 tgagaatcct ggttaatgtg atttaaatca atttttaaat attttctcta cgcaaatatt
    54841 aactcgatcg ccacttgccg cgctttgccc acgcctgaag gccagcaatt acctccgctt
    54901 tacaaagata tgcttttcca tacaagacct gatgctgcca cccgaggctt aaccagggta
    54961 caattgcccg ctaattaaca cctgcataaa actcaaggag agtgcatgtc tatcacggcg
    55021 cagtccgtat accgtgacac cggaaatttc ttccgtaatc aatttatgac cattctgttg
    55081 gtatcgttgc tatgtgcgtt tatcacagtg gtgttagggc atgttttctc acccagtgat
    55141 gcacagcttg cgcagctcaa tgacggcgtg cccgttagcg gcagtagtgg gttgttcgac
    55201 ctggttcaga atatgtcacc ggaacagcaa caaattttgc tgcaggcttc agcggcgtcc
    55261 actttttcag aattaatcgg taacgccatt ctcgccggag gcgtaatatt aattatccag
    55321 ctggtgtctg cgggtcagag agtcagtgcg ctgcgagcta ttggtgccag tgcgccgata
    55381 ttgcccaagt tatttattct gatttttctg actacccttt tagtacagat tggcatcatg
    55441 ctggtggtcg ttccgggaat tatcatggcc attttactgg ccctggcacc ggtgatgctg
    55501 gtacaggaca aaatgggcat ttttgcctcg atgcgtagca gtatgcggct gacttgggcg
    55561 aatatgcgtc tggtggcacc cgcagtactg agctggttgc tggcaaaaac actgttgctg
    55621 ctttttgcct cttcttttgc cgcattaacc ccggaaattg gtgccgtact ggcgaacacc
    55681 ttgagcaacc tgatttctgc catattgctc atctatctgt tccgcctgta tatgttgatt
    55741 cgccaataac cttaagcatt tgattacgga atcgtaaaat gaagcagttt cttgattttt
    55801 taccgctggt tgtctttttc gcgttttaca agatttatga catctatgcg gctactgcag
    55861 cgctgatcgt cgccacggcg attgtgctta tctatagctg ggttcgcttt cgtaaggttg
    55921 agaagatggc cctgatcact tttgttctgg tggtcgtctt cggtggcttg acgctgttct
    55981 tccacaatga tgagtttatt aaatggaagg ttacagtcat ttatgctctg tttgcgggag
    56041 ccctgttagt cagccaatgg gtgatgaaaa agccgctaat tcagcggatg ctgagtaaag
    56101 aactcacgct gccgcaaccg gtatggtcga agctaaatct ggcctgggct gttttcttta
    56161 tcctttgcgg tctggcaaac atctacatcg cattctggct gccgcaaaat atttgggtca
    56221 actttaaagt ctttggcctg accgccctta ccttaatctt cacattgtta agcggtatct
    56281 atatctaccg ccacatgccg caggaagata aatcctaacc agactgccag tccttcgggg
    56341 ctggcgctgt atctcctctg ttctcctcag gaaaatcata gtagcatcgc gcctgtgatt
    56401 ttccttttaa gtcggtttta ccatgtctac aacacataac gtccctcagg gcgatcttgt
    56461 tttacgtact ttagccatgc ccgccgatac caatgccaat ggtgacatct ttggtggttg
    56521 gttaatgtca caaatggata ttggcggcgc tattctggca aaagaaattg cccacggtcg
    56581 cgtagtgact gtgcgggttg aaggaatgac tttcttacgg ccggttgcgg tcggcgatgt
    56641 ggtgtgctgc tatgcacgct gtgtccagaa agggacgaca tcggtcagca ttaatattga
    56701 agtgtgggtg aaaaaagtag cgtctgaacc aatcgggcaa cgctataaag cgacagaagc
    56761 attatttaag tatgtagcgg ttgatcctga aggaaaacct cgcgccttac ctgttgagta
    56821 atagtcaaaa gcctccggtc ggaggctttt gactttctgc ttactgaatt tcggtggtgc
    56881 cgttaatttt aaacaggata ttcaccacaa tcccactgcc tggcttaccc ggctcataac
    56941 gccatctgcg catcgcattt ttcacctcac gctcaaacat gttcgcaggc ttggctgaga
    57001 ggatttgtac gttatccacg cgaccatccg gcgtgacgtc aaatttaact ttaacctgcc
    57061 cttcaatgcg caatgcctgt gctcgtgccg gatactgcgg ctgattacgg cttaatgcgc
    57121 gtggtcctga agccacactg gtaaccggct tgctggttgc agccgttgcc gtacttgatg
    57181 tggggcgcgc gggtgccgta ttttcaaacg gtgatgccgg acgcgactct acgggtttga
    57241 catcgcgttt tggctgctcc tgtacctttt tcaccggctt cggttttggc ttaggtttcg
    57301 gcttcggctt ttcaatgacc accggtgctt cttttggcgg ttcggggatc ggctcaggtt
    57361 ccggttctgg ctctaccacc ggctccggtg gcggctgaac ggcttgtggc ggttcgagat
    57421 cagcaggcgc aaccatcgtg acagaaattg gttgcgcagg cgcaggtagt tcaataacct
    57481 gatgtaccga ggtatagagc agccccgcca caacagcacc atgaatgcag accgaaagta
    57541 acgtcggcca ggggaagcgg cgaggtaaat caagggttat tgaagtcata atcatttcag
    57601 tagaaaaact aggcctcgat tttaaatgca aatagcaatc atattcaata aggcaagacg
    57661 atcaggatca gtgaaaatgt catttttggc gtaattaccc ttactttatg catgacctta
    57721 acaaagacat catctttccg ttgcagatct tcgccctttc aaataacgta ctttacaact
    57781 ttcccgaaca aggagttgtg cccgtgttgt atgttattta cgctcaagac aaagctgata
    57841 gcctcgaaaa acgcctttcc gttcgtccgg cacatttagc acgtttacag ttactgcatg
    57901 atgaaggtcg tttgttgaca gctggtccaa tgccagcggt agacagtaac gatccagggg
    57961 ctgcgggatt tactggctca accgttattg ctgaatttga atcgctggaa gcagctcagg
    58021 cctgggccga tgctgacccg tacgtagcgg caggagtcta tgaacacgtt tcagtgaaac
    58081 catttaagaa agtgttctga g
//
LOCUS       DAJTRD010000015        58032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTRD010000015 DAJTRD010000000
VERSION     DAJTRD010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.015"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(24..278)
                     /locus_tag="OO315_001008"
     CDS             complement(24..278)
                     /locus_tag="OO315_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5890014.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            433..1005
                     /gene="yeiP"
                     /locus_tag="OO315_001009"
     CDS             433..1005
                     /gene="yeiP"
                     /locus_tag="OO315_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5890015.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1228..2694
                     /locus_tag="OO315_001010"
     CDS             1228..2694
                     /locus_tag="OO315_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5890016.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2812..3798
                     /gene="yeiR"
                     /locus_tag="OO315_001011"
     CDS             2812..3798
                     /gene="yeiR"
                     /locus_tag="OO315_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5890017.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3837..4550
                     /gene="lpxT"
                     /locus_tag="OO315_001012"
     CDS             3837..4550
                     /gene="lpxT"
                     /locus_tag="OO315_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5890018.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4962..5528
                     /gene="mepS"
                     /locus_tag="OO315_001013"
     CDS             4962..5528
                     /gene="mepS"
                     /locus_tag="OO315_001013"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5890019.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5709..7265
                     /locus_tag="OO315_001014"
     CDS             5709..7265
                     /locus_tag="OO315_001014"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5890020.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7347..9161
                     /locus_tag="OO315_001015"
     CDS             7347..9161
                     /locus_tag="OO315_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5890021.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9162..10256
                     /locus_tag="OO315_001016"
     CDS             9162..10256
                     /locus_tag="OO315_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5890022.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10256..11281
                     /locus_tag="OO315_001017"
     CDS             10256..11281
                     /locus_tag="OO315_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5890023.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11283..12872
                     /gene="yejF"
                     /locus_tag="OO315_001018"
     CDS             11283..12872
                     /gene="yejF"
                     /locus_tag="OO315_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5890024.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12876..13220)
                     /locus_tag="OO315_001019"
     CDS             complement(12876..13220)
                     /locus_tag="OO315_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890025.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO315_001020"
     CDS             complement(13553..14743)
                     /gene="bcr"
                     /locus_tag="OO315_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5890026.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVCRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO315_001021"
     CDS             complement(14771..15466)
                     /gene="rsuA"
                     /locus_tag="OO315_001021"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5890027.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15616..17376
                     /locus_tag="OO315_001022"
     CDS             15616..17376
                     /locus_tag="OO315_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5890028.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17501..17785
                     /gene="rplY"
                     /locus_tag="OO315_001023"
     CDS             17501..17785
                     /gene="rplY"
                     /locus_tag="OO315_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5890029.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO315_001024"
     CDS             complement(17924..18931)
                     /gene="yejK"
                     /locus_tag="OO315_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5890030.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19113..19340
                     /locus_tag="OO315_001025"
     CDS             19113..19340
                     /locus_tag="OO315_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5890031.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19360..21120
                     /gene="pbgA"
                     /locus_tag="OO315_001026"
     CDS             19360..21120
                     /gene="pbgA"
                     /locus_tag="OO315_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5890032.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21195..21271
                     /locus_tag="OO315_001027"
     tRNA            21195..21271
                     /locus_tag="OO315_001027"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21229..21231,aa:Pro,seq:ggg)
     gene            complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO315_001028"
     CDS             complement(21374..23965)
                     /gene="yejO"
                     /locus_tag="OO315_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5890033.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24285..24932
                     /gene="narP"
                     /locus_tag="OO315_001029"
     CDS             24285..24932
                     /gene="narP"
                     /locus_tag="OO315_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5890034.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO315_001030"
     CDS             complement(25143..26195)
                     /gene="ccmH"
                     /locus_tag="OO315_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5890035.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO315_001031"
     CDS             complement(26192..26749)
                     /gene="dsbE"
                     /locus_tag="OO315_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5890036.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO315_001032"
     CDS             complement(26746..28689)
                     /gene="ccmF"
                     /locus_tag="OO315_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5890037.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO315_001033"
     CDS             complement(28686..29165)
                     /gene="ccmE"
                     /locus_tag="OO315_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5890038.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO315_001034"
     CDS             complement(29162..29371)
                     /gene="ccmD"
                     /locus_tag="OO315_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5890039.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO315_001035"
     CDS             complement(29368..30105)
                     /gene="ccmC"
                     /locus_tag="OO315_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5890040.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO315_001036"
     CDS             complement(30147..30809)
                     /gene="ccmB"
                     /locus_tag="OO315_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5890041.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO315_001037"
     CDS             complement(30806..31423)
                     /gene="ccmA"
                     /locus_tag="OO315_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5890042.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO315_001038"
     CDS             complement(31442..32044)
                     /gene="napC"
                     /locus_tag="OO315_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5890043.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO315_001039"
     CDS             complement(32054..32503)
                     /gene="napB"
                     /locus_tag="OO315_001039"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5890044.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO315_001040"
     CDS             complement(32500..33363)
                     /gene="napH"
                     /locus_tag="OO315_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5890045.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO315_001041"
     CDS             complement(33350..34045)
                     /gene="napG"
                     /locus_tag="OO315_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5890046.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO315_001042"
     CDS             complement(34052..36538)
                     /gene="napA"
                     /locus_tag="OO315_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5890047.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO315_001043"
     CDS             complement(36535..36798)
                     /gene="napD"
                     /locus_tag="OO315_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5890048.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO315_001044"
     CDS             complement(36788..37282)
                     /gene="napF"
                     /locus_tag="OO315_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5890049.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37391..37555
                     /locus_tag="OO315_001045"
     CDS             37391..37555
                     /locus_tag="OO315_001045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890050.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37690..38178
                     /gene="eco"
                     /locus_tag="OO315_001046"
     CDS             37690..38178
                     /gene="eco"
                     /locus_tag="OO315_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5890051.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38300..39331)
                     /locus_tag="OO315_001047"
     CDS             complement(38300..39331)
                     /locus_tag="OO315_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5890052.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39565..39924)
                     /locus_tag="OO315_001048"
                     /pseudo
     CDS             complement(39565..39924)
                     /locus_tag="OO315_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO315_001049"
     CDS             complement(40142..41785)
                     /gene="yojI"
                     /locus_tag="OO315_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5890053.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO315_001050"
     CDS             complement(41861..42511)
                     /gene="alkB"
                     /locus_tag="OO315_001050"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5890054.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO315_001051"
     CDS             complement(42511..43575)
                     /gene="ada"
                     /locus_tag="OO315_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5890055.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO315_001052"
     CDS             complement(43649..44704)
                     /gene="apbE"
                     /locus_tag="OO315_001052"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5890056.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO315_001053"
     CDS             complement(44816..45937)
                     /gene="ompC"
                     /locus_tag="OO315_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5890057.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46675..49347
                     /gene="rcsD"
                     /locus_tag="OO315_001054"
     CDS             46675..49347
                     /gene="rcsD"
                     /locus_tag="OO315_001054"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5890058.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49364..50014
                     /gene="rcsB"
                     /locus_tag="OO315_001055"
     CDS             49364..50014
                     /gene="rcsB"
                     /locus_tag="OO315_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5890059.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO315_001056"
     CDS             complement(50214..53063)
                     /gene="rcsC"
                     /locus_tag="OO315_001056"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5890060.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53338..54114)
                     /locus_tag="OO315_001057"
     CDS             complement(53338..54114)
                     /locus_tag="OO315_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5890061.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54119..55768)
                     /locus_tag="OO315_001058"
     CDS             complement(54119..55768)
                     /locus_tag="OO315_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5890062.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55769..56328)
                     /locus_tag="OO315_001059"
                     /pseudo
     CDS             complement(55769..56328)
                     /locus_tag="OO315_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56332..56490)
                     /locus_tag="OO315_001060"
     CDS             complement(56332..56490)
                     /locus_tag="OO315_001060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890063.1"
                     /translation="MTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGG
                     GEDWRWVG"
     gene            complement(56487..>58032)
                     /locus_tag="OO315_001061"
     CDS             complement(56487..>58032)
                     /locus_tag="OO315_001061"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5890064.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14196 a        15655 c        14259 g        13922 t
ORIGIN      
        1 tgcttactgc ggattaaaga tgtttaaggc gcggtcagca tttccagatc gataagccag
       61 gtcatggcct gctgacgcga gttaccgcac atttcagcgc tggcttgtaa gcctgcacag
      121 actttcggtc gcagtggcga ggtgaaaatt ttacagcgtt gctgttcatc aagctgaatg
      181 cagggcgtat tggcgggctt gccatctggc ataccgggaa tagggctgga aattgaaggg
      241 gcagtgcaac acgccccgca acccggacgg cattccatac gttgttctct taaggttaac
      301 caccggattg atgagcgcgc acagtaccac cttttttgca ccagcaaaag tgcgaatacc
      361 acttgcccga aaggcccgtc gcgagtactt tgtcgcgata tttttgacat tttcgactac
      421 aggaattttt cgatgccaag agcgaacgaa attaaaaaag gtatggtact gaattacaac
      481 ggcaaactgc tgctggctaa ggatattgat attcagtcgc ccactgcccg cggtgccgct
      541 acgctgtaca aaatgcgttt ttctgatgtc cgtaccgggc tgaaagtaga agagcgtttc
      601 aaaggtgatg atatcgttga caccgtgacg ctgacccgcc gttacgttga cttctcctat
      661 gtcgatggca acgaatatgt ctttatggat aaagaagact ataccccgta taccttcacc
      721 aaagatcaga ttgaagaaga gttgctgttt atgccagagg gcggcatgcc ggacatgcag
      781 gtgctgacct gggacggtca actgctggcg cttgagctgc cgcagaccgt tgatctggaa
      841 atcgttgaaa ccgcaccagg catcaaaggg gcatctgcca gcgcccgtaa caaaccggcg
      901 acattgagca ctggtctggt gattcaggta ccagaatact taagcccggg cgaaaaaatt
      961 cgtatccata tcgaagaacg ccgttatatg gggcgtgctg actaacttca gccgcatgca
     1021 gaaaagggat agctcaggct gtcccttttt taatttatta taccaatctt ctattttgcg
     1081 cttcctgaac accttacgcc ctcaattttc actcgttgat tgatcgccct cacacttcat
     1141 cgcattaaca atccagacca atttcaattg ctgtcatata actttacact gtcattgtta
     1201 atttatcgtt actaagacgt gactcctatg aagacaattg cctccgttac gctcccgcat
     1261 catgtacacg ctccacgcta cgatcgccag cagttgcaat cacgtatcgt tcattttggc
     1321 tttggcgcct ttcaccgcgc tcatcaggcg ttactgaccg atcgtgtgct gaatgcccag
     1381 ggcggcgact gggggatctg tgaaatcagc ttgttcagcg gtgatcaact gatgagccag
     1441 ctccgcgcac agaaccattt atataccgtg ctggagaaag gtgcggacgg caatcaggtg
     1501 ataattgtcg gtgccgttca cgaatgcctt aatgcaaaac tggattcctt agcggcaatt
     1561 attgagaaat tttgcgagcc acaggtggca attgtttccc tgacgattac cgaaaaaggc
     1621 tattgtattg acccggccac cggtgcactc gacaccagta atccgcggat tattcacgat
     1681 ctacaaaccc ctgaagaacc tcactccgca ccgggtattc tcgtcgaagc actgaaacgc
     1741 cgccgtgagc gcagccttac accgtttacc gtgctctcct gcgacaatat tcccgacaat
     1801 ggccatgtgg tgaaaaacgc ggtgctgaga atggcagaaa aacgttcgcc agaactcgcc
     1861 gggtggataa aagaacacgt cagttttccg ggaaccatgg tcgaccgcat tgttccggct
     1921 gcaaccgacg aatcactggc agaaatcagc cagcatctgg gggtaaatga tccctgcgcg
     1981 attagctgcg aaccgtttat ccagtgggtg gttgaagata acttcatcgc tgggcgtcct
     2041 gcctgggaag tcgcaggtgt acaaatggtg aatgatgtcc tgccatggga agagatgaaa
     2101 ctgcgtatgc ttaatggcag ccactctttt ctcgcttatc tgggttacct ctcaggattc
     2161 gcctatatca gtgattgcat gcaggatcgc gtatttcgct atgctgccag aacattaatg
     2221 ctggatgagc aagcgccgac actgcaaatt aaagatgtcg atttaacaca atatgcggat
     2281 aagttaattg cacgttttgc taatccgtcg ctgaaacata agacctggca aatcgcgatg
     2341 gatggcagcc agaaattacc gcaacgtatg ctggcaggta ttcgcataca tctggggcgt
     2401 gaaacggact ggtcgttgct ggcattaggc gttgctggct ggatgcgtta cgtcagcggc
     2461 gttgatgatg ccggaaatgc cattgatgtt cgcgatccgc ttagcgataa aattcgcgaa
     2521 cttgttgcgg gcagcagcag tgaacaacgc gtaaccgccc tgctttccct gcgtgaagtt
     2581 ttcggtgatg atctgccaga taacccgcat tttgtgcagg ccatcgaaca agcctggcaa
     2641 caaatcgtac aattcggcgc acatcaggcg ctattaaaca ccctcaaaat ttaacgattt
     2701 ctgcggttaa agcggatgaa gctcaccttc gtccgctctc cccttctctt ttctgccttt
     2761 tttagccagg attaacgctc agttaactta ccagagtatt gcggagccgt tgtgaccagg
     2821 accaacctca tcaccggttt tctcggcagc gggaaaacca cgtcgattct tcatctgtta
     2881 gcccataaag atcccaacga aaaatgggcg gtactggtta atgaatttgg ggaagtcgga
     2941 attgatggtg ctttgctcgc cgatagcggc gcattgctga aagagatccc cggcggctgc
     3001 atgtgctgcg ttaatggttt acccatgcag gtagggttga ataccttact gcgtcaggga
     3061 aaaccagacc gcttgttgat agagccgacc gggctgggcc atccgaaaca gatcctcgat
     3121 ctgttaaccg caccagtcta tgaaccgtgg atagatctgc gcgccacctt gtgcattctc
     3181 gatccacgcc tgctgctgga cgaaaaaagc gccagcaatg aaaacttccg tgaccagctg
     3241 gctgccgcag acatcattgt cgccaataaa tccgaccgtg cgacgcccga aagtgagcaa
     3301 gcgctacagc gttggtggca gcaaaatggt ggcgatcgac aattaattca cagtgagcat
     3361 gggaaagttg acggtcatct tctggatttg ccgcgtcgca atttagccga gttgcccgcc
     3421 agcgccgcgc attctcatca gcatgtcgtg aaaaaagggt tagcagcgtt aagcctgcca
     3481 gagcatcaac gctggcgtcg cagtctgaac agcgggcaag gatatcaggc ctgcggctgg
     3541 atattcgacg ctgatacggt attcgacacc attggcattc tggaatgggc gcgacttgca
     3601 ccggtggaac gcgtcaaagg cgtgctgcgt attcccgaag ggctggtacg aatcaaccgt
     3661 cagggcgatg acctgcacat tgaaacgcaa aacgttgcgc caccggacag ccgtattgag
     3721 ctgatttcca gcagcgaagc tgactggaat gccttgcaga gcgcgctgtt gaagcttcgt
     3781 ttagcgacta ccgcgtaagg ttgcctgcgt ttttcagtaa gataattaga gaaaatatga
     3841 ttaaaaattt gccgcaaata gtgttgttga atattgtcgg ccccgcgctg tttctttcct
     3901 ggtatattcc cgttaatcat ggattctggt tgccgattga tgcggatatt ttttatttct
     3961 ttaatcagaa actggtcgaa agtaaggcct ttttatggct ggttgcattg actaacaatc
     4021 gcgccttcga cggttgttca ctgctggcga tgggtatgtt gatgctgagt ttctggctga
     4081 aagaaaacgc ccctggcaga cgacgtatcg tgattatggg tctggtcatg ctattaactg
     4141 cagtggtatt aaaccagctg ggtcaggcat taattcctgt aaaacgggcc agcccaacat
     4201 tgacttttac cgatattaac cgcgtcagcg aactgctctc tgttcccacg aaagatgcct
     4261 cacgagatag ctttcccggc gatcacggca tgatgctgct tattttttcg gcattcatgt
     4321 ggcgttattt cggcaaagtt gcaggcctta tcgcccttat tatttttatg gtttttgcat
     4381 ttcccagagt aatgattggc gcacactggt ttactgacat cattgtcggt tcgatgaccg
     4441 tgatattgat cggtttgccc tgggtgttgc tgacgccatt aagtgatcga ttaatcacct
     4501 tttttgacaa atcactacca ggaaaaaaca aacatttcca aaacaaataa ctcacagtaa
     4561 ttaacatcat cagggttatt tttatagtga ggataatcct gatgatgcgc accgtgcttt
     4621 catctatcga acgcaaaaat cattctctaa gtaaatgaat ggattgcatg cgtttcactc
     4681 aattgtactt taattgacca accccgctta ttaactttct gtatcacttt ttcttataaa
     4741 aaatcatgta aaaccgctcg ccaagaccgc accaatcggg taatctcgaa ctcgttttgc
     4801 ctcggcggta gattatcctc acagcatata attttgtgcg ttagtccaca gatttggcct
     4861 taaggaattg tttcaacatg cccaggtaat tagtctcgtg tcgcttggca tttttttata
     4921 acgatatttg tcgttaagga cttcaaggga aaacaaacaa catggtcaaa tctcaaccga
     4981 ttttgagata tatcttgcgc gggattcccg cgattgcagt agcggttctg ctttctgcat
     5041 gtagtgcaaa taacaccgca aagaatatgc atcctgagac acgtgcagtg ggtagtgaaa
     5101 catcatcact gcaagcttct caggatgaat ttgaaaacct ggttcgtaat gtcgacgtaa
     5161 aatcgcgaat tatggatcag tatgctgact ggaaaggcgt acgttatcgt ctgggcggca
     5221 gcactaaaaa aggtatcgat tgttctggtt tcgtacagcg tacattccgt gagcaatttg
     5281 gcttagaact tccgcgttcg acttacgaac agcaggaaat gggtaaatct gtttcccgca
     5341 gtaatttgcg tacgggtgat ttagttctgt tccgtgccgg ttcaacggga cgccatgtcg
     5401 gtatttatat cggcaacaat cagtttgtcc atgcttccac cagcagtggt gttattattt
     5461 ccagcatgaa tgaaccgtac tggaagaagc gttacaacga agcacgccgg gttctcagcc
     5521 gcagctaata aaccgtttgg atgcaatccc ttggctatcc tgacgagtta actgaaagca
     5581 ctgcttaggc agtgcttttt tgttttcatt catcagagaa aatgatgttt ccgcgtcttg
     5641 atccaggcta tagtccggtc attgttatct tttaaatgtt gtcgtaattt caggaaatta
     5701 acggaatcat gttcatacgc gctcccaatt ctggacgtaa gctcctgctt acctgcattg
     5761 tagcaggcgt gatgattgcg atactggtta gctgccttca gtttttagtg gcctggcata
     5821 agcacgaagt caaatacgac acactgatta tcgacgtaca aaagtatctc gatacctatt
     5881 ttgccgacct gaaatccact actgaccggc ttcagccgct gaccttagat acctgccagc
     5941 aggctaaccc cgaactgacc gctcgcgcgg cgtttagcat gaatgtccgt acgtttgtgc
     6001 tggtgaaaga taaaaaaaca ttctgttcat ctgcgactgg tgagatggac attccactca
     6061 atgaattgat tccggcgctc gacattaata aaaacgtcga tatggcgatc ttacccggca
     6121 cgccgatggt gccgaacaaa cccgcaatcg tcatctggta tcgcaaccct ttgctgaaaa
     6181 atagcggtgt gtttgccgct ctgaatctca acctgacgcc ttctctcttt tatagttcac
     6241 ggcaggaaga ttacgatggc gtcgccctca ttattggcaa tactgcgcta tctacctttt
     6301 cttcacgttt gatgaacgtt aatgaattaa ccgacatgcc ggtccgtgaa actaaaattg
     6361 cgggcattcc tctgaccgtt cggctttatg cggatgactg gacatggaac gatgtgtggt
     6421 acgcattttt actgggcggc atgagtggaa ctgtcgttgg cctgctttgc tattacctga
     6481 tgagcgtacg tatgcgcccc gacagagaaa tcatgaccgc catcaagcgc gaacaatttt
     6541 acgtggcgta tcaaccggtg gtggatacac aagctttgcg ggtaacgggc ctggaagtac
     6601 tgctacgctg gcggcatcct gtagcgggag aaattccccc ggatgccttc attaactttg
     6661 ccgaatcgca aaagatgatt gtgccgctga ctcagcacct gtttgagtta attgcccgcg
     6721 atgccgcaga attagaaaaa gtactgccgg taggcgtcaa atttggcatt aacattgcgc
     6781 cggaccatct gcacagcgaa agctttaaag cagatatcca gaaactgctc acttccctgc
     6841 ccgcacacca tttccagatt gtgctggaaa ttaccgagcg cgatatgctg aaagagcaag
     6901 aagccacaca actcttcgcc tggctgcact cggtcggcgt agaaattgct attgatgact
     6961 tcggcaccgg gcacagcgcg cttatctatc ttgagcgttt tacgctcgat tatctgaaaa
     7021 ttgaccgtgg atttatcaac gccatcggta cggaaacgat cacttcaccc gtacttgacg
     7081 cggtgctgac gctggcgaaa cgtctcaata tgctgacagt tactgaaggg gtcgaaacgc
     7141 cggaacaagc gcgatggcta agcgaacgcg gcgttaattt catgcaaggc tactggatta
     7201 gtcacccgtt accgctggac gattttgttc gctggctgaa gaaaccgtat acgccgcagt
     7261 ggtaaggtgt gcttacgtcc cttattattc atagtgaaag catgccggat tgcggctaat
     7321 gatgagtaaa aggaaatccg ttgcagatga ttgtgcgcat actgctgctg tttatcgctc
     7381 tgttcacctt cggtgcgcag gcgcaggcta tcaaggaaag ctataccttt gccgtgctgg
     7441 gcgaaccccg gtacgcgttt aatttcaacc attttgatta tgtgaacccc gccgcgccaa
     7501 aaggtgggca gataacgttg tcagccctcg gtaccttcga taatttcaac cgctatgcac
     7561 tgcgcggcaa cccgggcgca cgcaccgagc agctgtacga cacgttattt acgacttccg
     7621 atgacgaacc aggcagttat tacccgctga ttgctgaaag tgcacgctat gctgacgatt
     7681 attcctgggt ggaggtcgct attaatccgc gcgcccgttt tcatgatggt tctcccatta
     7741 ctgcccgcga tgtagagttt acttttcaaa aatttctgac cgaaggcgtg ccgcaatttc
     7801 gtctggtcta caaaggcacc accgtcaaag ccattgcgcc gttaaccgtg cgcattgagt
     7861 tagctaaacc cggcaaagaa gatatgttga gtctgttttc gctgccggta tttccagaaa
     7921 agtactggaa ggatcacaaa cttagcgacc cgctcgccac gcctccgctt gccagtggtc
     7981 cgtaccacgt tacgtcctgg aaaatggggc aaaatattgt ctattcccgc gtgaaagatt
     8041 actgggcagc aaacttaccg gtaaaccgtg gacgctggaa tttcgacacc attcgctacg
     8101 attattacct cgatgataat gtcgcctttg aagcgtttaa agcaggtgcc tttgatttgc
     8161 gtatggaaaa cgacgctaaa aactgggcca cgcgttatac cggtaaaaat ttcgataaaa
     8221 aatacatcat caaagatgag caaaagaacg aatcagccca ggatacgcgc tggctggcgt
     8281 ttaatatcca acgtgcggta ttcagcgatc gccgggtgcg ggaagcaatc actctcgcct
     8341 ttgactttga atggatgaac aaggcgttgt tttacaacgc ctggagtcgc acgaacagtt
     8401 attttcaaaa taccgaatac gcggccagaa attaccccga cgccgcggag ctggtgcttc
     8461 tggcaccaat gaaaaaagat ctaccgccag aagtcttcac acaaatctac cagccgccgg
     8521 tatctaaagg cgatggctac gatcgtgaca acctgttaaa agccgacaaa cttctcaacg
     8581 aagcgggctg ggtgctgaag ggtcagcaac gcgttaatgc cacaacgggt cagccgctca
     8641 gctttgaatt attgcttccc gcaagcagca atagtcagtg ggtattgccg ttccagcaca
     8701 gcctgcaacg gctgggtatc aatatggaca ttcgcaaggt ggataactcg caaatcacta
     8761 accgcatgcg caatcgcgac tatgacatga tgccgcgact atggcgggcg atgccgtggc
     8821 ccagttccga tttacagatt tcctggtcat cggaatatat caattccact tataatgccc
     8881 ccggcgtgca aagcccggtt atcgactcac tgatcaacca aattattgcc gcgcagggaa
     8941 ataaagaaaa attactgccg ttggggcgag cactggatcg cgtattaacg tggaattatt
     9001 acatgctgcc aatgtggtac atggcggaag accgtctcgc ctggtgggat aaattctccc
     9061 aacccgctgt acgccctgtt tacagcctgg gtatcgatac ctggtggtat gacgttaata
     9121 aagcggccaa actgccgtca gccagacaac agggagagta gatgggcgct tacctgattc
     9181 gccgtctgtt gctggtgatc ccaacgttat gggctattat cactatcaac tttttcatcg
     9241 tgcaaattgc gcctggcggt ccggtcgatc aggccatcgc cgccattgag tttggtaatg
     9301 ccggagtatt acccggcgca ggcggtgaag gtgttcgtgc cagccatgcg caaacgggtg
     9361 tcggcaatat cagcgacagt aattaccgtg gcggacgcgg attagatcca gaagtgatcg
     9421 ctgagatcac tcatcgctac ggtttcgata agccgatcca cgaacgttac ttcaaaatgc
     9481 tctgggacta catccgcttt gattttggtg atagcctgtt tcgcagcgcc tcggtgctga
     9541 cgctgattaa agacagtctg ccggtttcca tcaccctcgg attgtggagc acgctgatta
     9601 tctatctggt gtcgattccg ttaggcattc gcaaagctgt ttataatggg agtcgctttg
     9661 acgtctggag tagcgcattt atcatcatcg gctacgccat tccggccttt ttgtttgcca
     9721 tcctgctgat tgtcttcttc gcgggcggca gctatttcga cctgttccct ctacgcggcc
     9781 tggtttccgc taactttgat tcgctgccgt ggtatcagaa aatcaccgat tatctgtggc
     9841 atatcacgct accggtgctg gcgacagtga ttggtggctt tgcggcgctg accatgctga
     9901 ccaaaaactc attccttgat gaagtgcgca agcaatacgt ggtgaccgcg cgtgcgaaag
     9961 gggtaagtga gaaaaacatt ctctggaaac atatgttccg caacgccatg ctgctggtga
    10021 ttgccggttt tccggcgacg tttatcagca tgttttttac cggctcgctg ctgattgagg
    10081 tgatgttttc actcaatggt cttggcttac tgggctacga agcgaccgtc tcgcgcgatt
    10141 atcctgtaat gtttggtacc ttgtatattt tcaccctgat tggcctgctg ctgaatattg
    10201 tcagtgatat cagctatacg ctggttgatc cgcgtataga ttttgaggga cgttaatgtc
    10261 gcgactcagc cccgtcaatc aggcccgttg ggcgcgtttt cgtcataacc gtcgcggcta
    10321 ctggtcgtta tggattttcc tcgttttgtt tggtttgagt ttgtgttctg aacttatcgc
    10381 taacgataaa ccgttgctgg tgcgttatga cggcagttgg tatttcccgt tgttgaaaaa
    10441 ctacagcgaa agcgattttg gcggcccgct ggcaagtcag gctgattatc aggacccgtg
    10501 gctgaaacaa cggctggaaa ataacggctg ggtactgtgg gcaccgattc gctttggtgc
    10561 taccagtatc aactttgcta ccgataagcc cttcccttct ccaccctccc ggcaaaactg
    10621 gctgggaacg gatgcaaacg gcggcgatgt gctggcacgc attctctatg gcacgcggat
    10681 ctcggttctg tttggcctga tgctgactct ctgttccagc gtgatgggcg tgctggcggg
    10741 ggcgctacaa ggctattacg gcggtaaagt cgatctctgg gggcaacgct ttattgaagt
    10801 atggtcgggg atgccgacgc tgtttttgat tattttactt tccagcgtcg tacagcctaa
    10861 cttctggtgg ctgctggcaa ttactgtctt gtttggctgg atgagtctgg tcggcgtggt
    10921 gcgggcggag tttttacgta ctcgtaattt cgactacatt cgtgcggcac aggcgcttgg
    10981 cgtcagcgat cgcagtatca tcctgcgtca tatgttgcct aatgccatgg tcgcgaccct
    11041 caccttttta ccgtttattt tatgtagttc gataaccacc ctgacctcgc tcgatttcct
    11101 cggcttcggt ctaccgctcg gttcaccgtc actcggtgaa ctgctgttac aagggaaaaa
    11161 taaccttcag gccccgtggc ttgggatcac cgccttcttg tcggtggcga tattattgtc
    11221 tttgctgatc tttattggtg aagccgtccg cgacgcattt gatcctaata aggcggtgta
    11281 gcatgacgca aactctgtta gcgattgaaa atttgtcggt gggttttcgc catcagcaaa
    11341 ccgtacgtac agtagtcaat gatgtttcac tacagattga ggctggcgaa acgctggcgc
    11401 tggtgggtga gtcaggttca ggcaaaagcg ttaccgcgct gtcaatttta cgcctgctcc
    11461 cttccccgcc ggttgaatat ctctccggcg atattcgttt tcatggcgaa tcgctgcttc
    11521 atgccagcga tcaaacgttg cgcggtgtac gcggtaataa gatcgccatg atttttcagg
    11581 aaccgatggt gtcgttaaat ccattgcata ccctggaaaa acagctttat gaagtgcttt
    11641 cactccaccg cgggatgcgt cgggaagcgg ctcgtggcga aattcttaac tgccttgatc
    11701 gcgtcggtat ccgccaggcg gcaaaacggc tgacagatta tccgcatcag ctctccggcg
    11761 gcgaacgcca gcgggtgatg attgcgatgg cgctgttaac gcgaccggaa ttattaattg
    11821 ccgatgaacc gaccaccgca ctggacgtct ctgtccaggc gcagatttta cagctgttgc
    11881 gcgaactgca aggcgagctg aatatgggca tgctgtttat tactcataac ctcagcattg
    11941 tcagaaaact ggcccaccgc gtggcggtaa tgcaaaacgg tcgctgtgtc gagcaaaata
    12001 acgccgctac gctatttgcc tcacccactc atccttacac acaaaagcta ctcaacagtg
    12061 aaccgtcagg cgatccggta ccgttgccag aacctgcctc aacgttgctg gatgttgaac
    12121 agcttcaggt tgccttcccc attcgcaaag ggattttgaa gcgcattgtg gatcataatg
    12181 tggtggtgaa aaacatcagt tttacgctac gggcgggtga aacactgggt ttagtgggcg
    12241 agtctggttc cgggaaaagc acgacgggac tggcgctgct gcgactgatt acctctcagg
    12301 gcagcatcgt ctttgacggt cagccactgc aagatttaaa tcgccgccag ctgttaccta
    12361 ctcgtcatcg tattcaggtg gtatttcagg atccaaactc ctcgctcaac ccacgactca
    12421 acgttttgca gattattgag gaaggcttac gggttcacca gccgacgctt tctgccgcac
    12481 aacgcgaaca acaagtgata gccgtgatgc atgaagtggg attagatcct gaaacacgcc
    12541 accgttatcc ggcggagttc tctggtggtc agcgacaacg tattgcgatt gccagggcat
    12601 taattcttaa gccctcgctg atcatacttg atgaaccgac atcatcactc gacaaaacgg
    12661 tacaggcgca aatattgacg cttttgaaat cattgcaaca aaagcatcaa ctggcctatt
    12721 tgtttatcag ccacgatttg cacgttgtcc gcgcgttatg tcatcaggtt atcgtactgc
    12781 gacaagggga agtagtggaa caaggaccgt gcgcgcgcgt gtttgccaca ccgcagcagg
    12841 agtatacgcg tcagttactg gcgttgagct gacgcttaaa aaggattgta gtttgaaaag
    12901 ggttcggcaa ttgccacacc aaaatttttc aatcggcatg ttgcagcaaa ctcgtcctgg
    12961 cgatttacaa acaggcacgg ctcaccttca cactccaaca ccgaacatgg aacttcgatg
    13021 tcgcttaacg cctggcttag tttatacatc accgaccatg cattatcacc atccgggcta
    13081 agcaatttaa gcgccaccag gctgttgtca ccgcacggtc cattttgcgg aatcggttca
    13141 accttcgaac ctgcgaaacc agcagaccag cgatagttct gcggcagtcg atggacgatt
    13201 gagagttgta atgaagtcac ttattttccc cggaagtaca ttcacttcac aatttgttta
    13261 catcaatttt aacacatcat caacaatccg tctttataca gatcgtaaag attgatgcct
    13321 cgcctgcgtg gcctctatgg ctctgattta agtaatatct gcgggcgtgg tcacgtttgc
    13381 ggggctatat gtacccgttt ctgcgatcta actcaacctt tttaactaca atgatgtgac
    13441 tttttacata aattgatttt acataaaata aacatatatc ggggaaatga tagatttgtg
    13501 gttgacatgc atcaacaaag gaagcctttt accttcctcg ttgtgcaata gatcaccgtt
    13561 ttttcggccg actggcgtac agacagaaga gaatggagct ggttgcgcag aatgcaattg
    13621 accaaatcat cggccatgca gagttaaagg tcgcaagaga aagcaatgcg ccaacaattg
    13681 cccctatgcc aaaacggaag gttcctgcca gcgaagatgc cgttcccgcc atatggggaa
    13741 actcatcaag aatgaccgcc atcgcattgg atgacaccat cgacacgcag cccacaaacg
    13801 ccgcaacgcc aaccaccagc gaccagaatc ccagccccag cggcgcactg atgaccatcc
    13861 acgcagccat aataaattgt atccacaacc ccgagcggaa catatttaac gcgccaatgc
    13921 ggcagacgaa gcggctgtta aagatggtca tcacgaacag aaaaacaatg tttagcgcaa
    13981 agtaataacc aaagttttcc ggcgcgacgt ggttaatttc aatataaaca aacggtccgg
    14041 cgcttaagaa tgagaacatc ccggcaaagc tgaaaccact ggcaagcatg tagctcagga
    14101 cacgtttatg gcggaacagc gccgcaaagt taccaatagt ggtacgaatg tgaaatggct
    14161 gacgacgctc cggtggtaag gtttctttaa tcaggaagaa aatcattgcc gaagccagaa
    14221 tcgccgctaa tgccaggatc cagaagatgt aatgccagct cagccacacc agcacccagc
    14281 cgccaactat cggtgccatc agcagtgcaa tggtcgtcac cagcatgaca aacgacatca
    14341 tccgcgagaa ctcttctttc gggtaaatat cacgcatcaa agcgttgata accacgctgg
    14401 ccgcagccgc agccagcccg tggaagaaac gcatcacaat cagctgatcg atggtttgtg
    14461 ccaacgcaca cgccaccgcg gcggcggcaa acaccagcgt cccgccaaga accaccggct
    14521 tacgcccgaa gctgtctgcc atcggcccgt agattaactg ccccaacgca aagcccagaa
    14581 tataagtact gagggtcatc tgcgtactgc ccgccgatac gccaaactgc gctgaaatta
    14641 ccggtagcgc gggcagatac atatcaatcg acagcggcat caacatggcc agcaggccaa
    14701 ggataaaaac aatagcaaac gacgaatgct gtcgggtggt cacaacgggc tcctgaaagt
    14761 cattgaaaag ttagacgacg ctggcaattt cttcttcagt taacggacga tattcaccgg
    14821 gggctaaatc agcatccagc gtaataccgc caatacgttc acgatgcagc tcaaccacgt
    14881 ggttacccac ggcggcgaac atgcgtttca cctgatgata acgcccttcg ctgatggtca
    14941 gacgaacctg cgttggggta atcacttcaa gcaccgccgg cttagtgaga tctttttcgt
    15001 tatgcagctg cacgccttta gcaaattgct ctgccgtatc gtcagctaca ggtgattcca
    15061 gtgtcaccag ataggtcttc tcgcaatgat ggcgcggaga agtaatgcgg tgcgaccact
    15121 gaccatcatc agtcatcagc accagaccgg tggtatcgat atccagacgt cctgcggcgt
    15181 gcagtttcca cgctaccggt tcatccagaa aatagagcac cgtcgggtga tccgggtcgt
    15241 ccgtggagca aacatagccc tgaggcttat tgagcatgaa gtaacgtgga ccgtgttgct
    15301 gcgccagcgg gttgccatcg taagcgacat catgttcagg gagcagtttg aacgctgcat
    15361 tacgaacgat ttcgccatcg acggtgacac gattgccgcg gatttcacgc ccggcaatag
    15421 cacggctaac gccgagttgc tgtgcgataa atttatcaag tcgcatgtgt gtgattttgc
    15481 ctgtaaaaaa cggaggtcgg gtaatgaccc gaaaatctga actgttgtct gcccagtata
    15541 gcggtctaat aacttccctc aagggaaaaa gaaccgtggc atactattag cagaataatc
    15601 tacctacgcg aaaccatgat ttttacactc cgcccatatc agcaagaagc cgtggatgcc
    15661 acgctcaacc attttcgtcg tcataaaacc cctgccgtta tcgtgctgcc caccggcgca
    15721 ggtaaaagcc tggtgatagc ggaactggca cggctggcgc gtggtcgcgt actggtgctg
    15781 gcacacgtta aagaactggt ggcgcaaaac catgcaaagt atcaggcgct ggggctggaa
    15841 gccgatattt ttgccgccgg gctaaagcgc aaagagagcc acggtaaagt ggtatttggc
    15901 agcgttcagt cggtcgcccg taatcttgat gcctttcagg gtgaattttc gctgttgatt
    15961 gtcgatgaat gccaccgtat tggtgacgat gaagagagcc agtatcagca aatcctcact
    16021 cacctgacta aagtgaatcc ccacttacgc ctgctggggc tgactgccac gccttttcga
    16081 ttgggcaaag gctggatcta ccagttccat tatcacggca tggtacgcgg cgatgagaaa
    16141 gcccttttcc gtgactgcat ttatgagctg ccgctgcgtt atatgattaa acacggctat
    16201 ctgacgccgc cagaacgact ggatatgcca gtagtgcaat acgatttcag ccgcttgcag
    16261 gcacagagta acgggctgtt cagcgaagct gatctcaacc gtgagctgaa aaaacagcaa
    16321 cgcattacgc cacatatcat cagccagatc atggagttcg ccgaaaagcg caaaggggta
    16381 atgattttcg ccgccaccgt tgaacacgca aaagagattg tgggattact gcctgccgaa
    16441 gatgctgcac tgattactgg cgacaacccc ggcgctgagc gcgatgtgtt aattgaagat
    16501 tttaaagccc agcgttttcg ctatctggtc aacgtcgcag tactgaccac cggatttgac
    16561 gccccgcacg tcgatcttat cgccattctg cgccctaccg aatcagtgag tctttaccaa
    16621 caaattgtcg ggcgcggtct gcgtctcgct ccgggcaaga ctgattgttt aattcttgat
    16681 tatgcgggta atcctcacga tctctacgcg ccggaagttg gtacaccgaa aggcaaaagt
    16741 gacaacgttc cggtacaggt tttctgccct gcctgcggtt ttgccaacac cttttggggg
    16801 aaaacgaccg ccgacgggac attgattgaa cactttggtc gccgctgtca gggatggttt
    16861 gaagatgacg acggtcatcg cgaacagtgt gacttccgtt tccgttttaa aaattgcccg
    16921 caatgtaacg cagaaaatga tattgccgct cgccgctgcc gcgaatgtga cacggtactg
    16981 gttgatccgg atgatatgtt aaaagcggcg ctacgactga aagatgcgct ggtattacgc
    17041 tgtagcggca tgtctttgca gcatgggcac gacgagaaag gcgaatggtt gaaaatcacc
    17101 tattacgatg aagacggcgc ggatgtgagt gagcgtttcc gtctgcaaac gcccgcccag
    17161 cgtaccgcct tcgagcagct ttttatccgc ccgcatacgc gcacaccggg catccctctg
    17221 cgctggatca ccgccgccga tatcctcgcc cagcaagcct tactgcgaca tccggatttt
    17281 gtcgtcgccc gcatgaaagg tcagtactgg caagtgcgtg aaaaagtgtt cgattacgaa
    17341 ggtcgttttc gtcgggcgca cgaattacgc ggttaatggt agttttgatt gatgtataag
    17401 gcgattgagt atagaatctc gcccgctttt gcatacgcaa agcagatcac ttacctgttg
    17461 ctgggtcgcc tgtagcagga ttaatttaag agagaaagaa atgtttacta tcaacgcaga
    17521 agtacgtaaa gagcagggta agggtgcgag ccgccgcctg cgtgccgcta acaagttccc
    17581 ggcaatcatc tacggtggca aagaagcacc gctggctatc gagctggatc acgacaaagt
    17641 catgaacatg caagctaaag ctgaattcta cagcgaagtt ctaaccatcg ttgttgacgg
    17701 taaagaaatc aaagttaaag ctcaggacgt acagcgtcac ccgtacaaac cgaagctgca
    17761 gcacatcgac ttcgttcgcg cttaattgct gaataagttg taaaaaaccc cgctccggcg
    17821 gggttttttg tatctgcaga ttatgcctga ttacggtatt gctatttttt gcaggccaga
    17881 taaggcgttc acgccgcatc tggcattcag aacacaaaac cgattaattg ccgccagacg
    17941 tccggcgctg caattggtcg cgcaaattcg gcggcgtgcc tttaatggtc aaggtatcgg
    18001 ttgctggatc ccagaagatg cgttcaccta acagcatcgc atcaaagtta atagtcaagc
    18061 caccgccgct accggcaaac ttcgtcaact gacgcagtgt gctacggtct gccgggaagc
    18121 tctcttccag ttcgtagcct ttttcagccg caaactccgt gaagctcact tcgcttacgc
    18181 ctgccagctc tttcgacagc gattccagtt cgatctcttc gcctgcctgc agctgctcat
    18241 tacagtagct ataaacctgc tggcgcacgt tctgtcgctc tgctttatcc agttgtgctt
    18301 cggcagtgaa atcatcaacc gcttgcaaca gcccacggtt ttgcgctttc gcgttcaatc
    18361 cttcgctggc cccgaggaaa tccataaaga aatccgccac tttgcgccct actcgccctt
    18421 tcaggaaagt gagatagcgg gtggactctg gattggtttc ccattcggtt aaatcgatac
    18481 gcgcaacaat atccgcatga ttgatatcaa gataatgggt tgggttgatg tcgagatttt
    18541 cgttaacgcg catactgctc aggttgctca gaactgccac cagcaaatac tccaccgcca
    18601 gataacggta gtggcaaaac agcacgaatc caccgtcagc aaaaggatat ttcgctaatt
    18661 cgtcacgcag gcgaccggtt gccgcgcggc taaatgccag aaagtcctct tcgccctgac
    18721 gctgtaagcg cagcgtctgc gccagttcac tctcttcgct aaacaagccg taagctttat
    18781 ttttggcgct atagacccga tgcagttctg ccaccatctc aacgacggtt tctgtcggtt
    18841 ccagcaatga atcgcgcaac accagctcaa ggttctgctc atcacgcttg ataagctggt
    18901 gcagagcaat ctggttgata tccagactca tgataaactc tcctttaaga ccgggcggta
    18961 ttcaaccacc gccagttaag cgacgcaata aaagatggcg gtcattttac agaaggcagc
    19021 gtaacgagaa tgatttaacg cataaaaaag cagaaaaaaa accgttgcta cggtaatatg
    19081 ttgccctttc atcaactaac tgatttcgat ttatgccaca aatttcccgc tacagtaatg
    19141 aacaggttga acaattgctc gcagagctgc tcaacgtact ggaaaaacat aaggctccga
    19201 ccgacctttc cctgatggtg ttaggcaaca tggtcactaa ccttatcaac accagcattg
    19261 ccccggccca acgccaggca attgccaact cttttgccag cgccttacag tcctctatca
    19321 acgaagacaa agcgcactaa gggaaacaga taacaggtta tggtaactca tcgtcagcgc
    19381 taccgtgaaa aagtctccca gatggtcagt tgggggcact ggtttgcact gttcaatatt
    19441 ctgctttcgc tcgtcattgg cagccgttac ctgtttatcg ccgactggcc gacaacgctt
    19501 gctggtcgca tttattccta cgtaagcatt atcggccatt tcagcttcct agtgttcgcc
    19561 acctacttgc tgatcctctt cccgctgacc tttatcgtcg gctcccagag gctgatgagg
    19621 tttttgtccg tcattctggc gacggcggga atgacgctat tactgatcga tagcgaagtc
    19681 tttactcgtt tccatctcca tcttaatccc atcgtctggc agttggttat caacccagac
    19741 gaaaatgaga tggcgcgcga ctggcagctg atgttcatca gcgtgccggt tattttattg
    19801 cttgaactgg tgtttgcgac gtggagctgg caaaagctgc gcagcctgac gcgtcgtcga
    19861 cgcttcgcgc gcccgctggc cgcattctta tttatcgcct ttatcgcctc gcatgtggtg
    19921 tatatctggg ccgatgccaa cttctatcgc ccgatcacca tgcagcgcgc taacctgccg
    19981 ctttcgtacc cgatgacggc gcgacgtttc cttgagaagc atggcctgct tgatgcgcag
    20041 gagtatcaac gccgtcttat tgagcaaggt aatccagacg ccgtttccgt acagtatccg
    20101 ttaagcgaac tgcgctatcg cgatatgggc accgggcaga atgtgctgtt gattactgtc
    20161 gatggcctga actactcacg cttcgagaag cagatgcctg cgctggcagg ttttgctgag
    20221 caaaatattt cgttcacgcg ccatatgagc tccggcaaca ctacagacaa cggcatcttt
    20281 ggcctgttct atggcatctc gccgagctat atggacggca ttctgtcgac ccgtacgcct
    20341 gcggcattaa ttactgcgct taatcagcaa ggctatcagc tggggttatt ctcatcagat
    20401 ggctttacca gcccgctgta tcgccaggca ttgttgtcag atttctcgat gccgagcgta
    20461 cgcacccaat ctgacgatca gaccgccacg cagtggatca actggctggg ccgctacgca
    20521 caagaagata accgctggtt ctcgtgggtt tctttcaatg gcactaacat tgacgacagc
    20581 aatcagcagg catttgcacg gaaatatagc cgggcggcag gcaatgtcga tgaccagatc
    20641 aaacgcgtgc tcaatgcact gcgtgattct ggcaaactgg acaatacggt ggtgattatc
    20701 actgccggtc ggggtattcc actgagcgaa gaggaagaaa cctttgactg gtcccacggt
    20761 catctgcagg tgccattagt gattcactgg ccaggcacgc cggcgcagcg tattaatgct
    20821 ctgactgatc ataccgatct gatgacgacg ctgatgcaac gcctgctgca tgtcagcaca
    20881 cctgccagcg aatattcgca aggtcaggat ttgttcaacc ctcaacgccg tcattactgg
    20941 gttactgctg cggataacga tacgctggca attaccaccc cgaaaaagac gctggtgctg
    21001 aacaataacg gtaaataccg cacttacaac ttacgtggtg aaagagtgaa agatgaaaaa
    21061 ccacagttaa gtttgttatt gcaagtgctg acagacgaga agcgttttat cgctaactga
    21121 ttaattataa atcagttagc gaaatatctt acttgcaatc ggtgtggaaa acggtagtat
    21181 tagcagccac gagtcggcac gtagcgcagc ctggtagcgc accgtcatgg ggtgtcgggg
    21241 gtcggaggtt caaatcctct cgtgccgacc aaaaatccca agaaaaaacc aacccttacg
    21301 gttggttttt ttatatctgc aattaattcg ataaacagac cgtgacacat cacagcctgt
    21361 ttattttctg ttatcagaac gtccagacca cacccgcctg agtattccac ggcgattcta
    21421 cccctgcgcc attgccatag ctgactgaca aatgaccgct taacgtcggg gtaaacgatg
    21481 accttatccc agcctgataa acgccactgg ttccagccac atcattatta aatttgccat
    21541 cgtcattaac tttcacctgg ttagaatcgg cgtattcctg acgcacggcg gctttcagcc
    21601 agggttccag cgtcgtaccg ttttgcaggt ccatgtgata gcttaccgcc gttcccgctt
    21661 cagcgcgtaa tatccgggta tttcccacat ccgcgcgcat gccgtttgat aacgtgtagt
    21721 cctgaccatc tgtggtaaag ccggtaaagg ccagataggg tctaacactc cacaatccgt
    21781 caacccaacg gaacccgctc tcaacatgag cacccgcgcc gttactattg taatcgccaa
    21841 acgctgttgc cccattactc atcttgccat ggatggtgtt ggcaaaacgg tcaactttca
    21901 ccaccccatc aacataggca ccgttctgat gctcccaacc ggcataagcc cccagggtat
    21961 agctatcgat attacctttt ccgccgcgat caaaaccaat atcagaatga gagtaaccaa
    22021 agaacaagcc gcgaattgta ctgctttctt cacgggagaa acggctatcg ataccgagcg
    22081 tcaggcccgt caatgtttgc tcaaaaccag ctcccgcatc agtggtcacg ttgttgcggg
    22141 tgttaattgc cgaactccac atcaccgtat cgtaactaac gccttttacg ctaccaagac
    22201 gctcacgcac ggtgtccagt tctgcatcaa ataccagcgg ttgtgcggct gccatgttca
    22261 gcacatcagt ggttgaaggg gtaatttgcg cgcgattctc tgcgaggctc cagctatggt
    22321 tgccattatc cagcaaggta tattcatacg taccgatatc aacaacgcct ccggcattgc
    22381 ccaacgtaaa tgcagcatca ccgccgcccg ttgttaccag tgtaaggcta tctcctgctg
    22441 ccgggctggc accggtgtcc gtcacgaata ttttgaaatc acctgttgcc tgaccggtga
    22501 cgttgagctg atcgctctga tgattggcca tatcggtacg catataaaaa ttgccgtttc
    22561 ccgacagggt attggtggtc aacgtaatga aattttccgc acttgccgtc acagatgaac
    22621 gagtcactgg ctcagccatc ataatcacat ttgcgccgga catcgcaagg ttggctaagt
    22681 tgaggcgagt gttttggttc gtcgtagtga tatcggcaat gatggtccat gcgctatttt
    22741 cgagcgaaac gtccgctttg ctggtatcca cggcaccatg cgttctaaaa gaagcgccct
    22801 cagaaaccac taccttaccc accaacatag cgggtgcata atttatttgc ggcttcatga
    22861 cctcaagaat gccatcattc cctctgactg acacgttaac cattgtgcca gcccagacat
    22921 tagcgcggcc attgttgatt accatatttt cagagatggc ttttgaggaa tacgtgatac
    22981 ttccattctg gtctgatcga ccgagatcat ataccgcatt tgcctgcact ttcgtaccag
    23041 tatcctttcc cagcgattgc atagtggcat gctcatcaag gatagtgtca atggcctgcg
    23101 tgtcctccat aacaatgagc ccggaaccat catccagttc ataatttttt gacacaccat
    23161 cctttatact aaattggcct ttactgtttg taccactcac ttccagcgca ttcgttgaga
    23221 caattaattt cccgcctgct tttttatcaa cgccagttgc agtcccgcca tccataacct
    23281 ccagtaagcc gccactatcc acagtagtat tataagcgaa gtcattttct tcaactcgca
    23341 aacttccgcc gttttccaac aacatattat tagcaatacc attcttgata tcgaactgac
    23401 cgagacggtt tgttccgaat acctccatgg cccgcgtggt tgttttaata gcaccgcctg
    23461 ttttctgatc taccgcaagc gcaaatcccc cctctttaac ctccagtagt cctccctttt
    23521 cgatagcggc aaaaacggcc cccccatctt tttcaacgat taagcgcccg cctttaatcg
    23581 tggtatcatt cgctgggcca taaacctgta gcacaccatt attgagggta gtctggtttg
    23641 ccgcgccacc catttgaaca atttgataac catcatttaa tactgagtat tctgcggtac
    23701 cattcatttc aatgtattga taccctccgt taatttcagt attactactt acaccaaatt
    23761 cttttatatg ctgttctccg ccaggattga ttttcgtatt attggcggtc ccataaacca
    23821 gctggatatc tttttccagg ataacaccat caactgtctc accatactca actgttgatg
    23881 ccaatgctat gggtgaatat aacgctgtag ccaccagaac agatattgcg gaacgacaaa
    23941 gagaaacaga accagattga tgcattgagc tttcatccta tgaaattaat tgcagttaaa
    24001 agcattgggt acagaaaata cccatagctc catacccgga gtcagttttt aaaaactgtt
    24061 taaagaaatg cacaagtatt gtgattgatt ttttagttgt ttttcttgat gagaagctga
    24121 tgcaaaattc cgtctttata ataaaaatga tgccaaagcg aacgacaagg ttgtagtttt
    24181 cactacatgt ccatacataa aatggggtaa cattcacgcg cctggtagcg ttaccaacgc
    24241 tacgctcaaa cataatgatt ctaataaaac ctcaggagac tactatgcct gaagcaacgc
    24301 cttttcaggt gatgattgtg gatgatcatc cacttatgcg acgcggtgtt cgtcagttac
    24361 tggagcttga tcctggcttt gaagtggtcg ccgaagcggg cgaaggcgcg agcgcgatcg
    24421 atctggcgaa tagactggat atcgacgtga tcttgctgga tctcaatatg aaaggtatga
    24481 gtggcctgga taccctcaat gccttgcgca gggatggcgt taccgcgcaa attattatcc
    24541 tgaccgtatc cgatgcctcc agcgatgtct ttgcgctgat agacgcaggc gcagacggtt
    24601 atctgttgaa agacagcgac ccggaagtat tgctggaagc gattcgtacc ggagcgaaag
    24661 gcagcaaagt ctttagcgaa cgcgtcaatc agtacttacg tgaacgtgaa atgtttggcg
    24721 cggaagaaga tcccttcagc gtgctgacgg agcgcgagct ggatgttctg cacgagctgg
    24781 cacaggggct gtcaaataaa cagattgcat cggtgttgaa tatttccgag cagacagtaa
    24841 aagtacatat tcgcaatctg ctgcgtaaac tcaatgtccg ctcacgcgtg gcggccacca
    24901 ttctgttcct gcaacaacgc ggggcacaat aaaaatagcc cgatggattt atcatcgggc
    24961 tgagatttat gacaaacgca aaactgcctg atgcgctacg cttatcaggc ctacgtggat
    25021 cgatcaattt attgaattta cacaattttg taggccggat aaggcgttca cgccgcatcc
    25081 ggcataaact aagcgcactt tgtcatcagt ctagcccgat ggcatcacca tcgggcctct
    25141 ttttatttac tctcctgcgg cgacaaatgt tgcatcgcct gcgcgatact acgttcaatc
    25201 accgcacggc gagtatcgtt ggcaggtaag agtttcaaca tcatctccca cgcggcaacg
    25261 gcttcgccaa atcgctgctg ctcaaaggca ttaaacgcat acatgcttag cacacggata
    25321 ttgctatggt ccgttctcac cagctgacgt agcagttcac cgccgaggcg gttgtcgttg
    25381 ggatcagatg aacgagtcaa cgcttcagcg tatcccagtg cggcatcgct gttcttcgga
    25441 tcgaggcgat acgcagtagc gtatgcatcg gtggcgatac tggcgttacc cagcgccatg
    25501 ccaacgcggc ccaacataat ccagccttct atatctcccg gatttttttg cagttgagta
    25561 cgcatcccca gcgcaaggcg cgacatctct tcttcgttga gcggatcggc tttcgggtcc
    25621 agcgccctgt ccagcaacgc cggagcctgt gccgtggcct gctgccagat tttcacctgc
    25681 tgataattgc cagtctggta gtagctgacg ccagccacaa ttaacgccac cacaataccc
    25741 ggcagataaa caacatatcc ggcacgctta ccttccggca cgctttgttc aggaaacgct
    25801 tccggcacca cgcgtacccg acgccgcgag cgggcgtata tgacccaacc gccaatgcca
    25861 atagccacta ctggcagcac ccacagcagc acggtcagcg gcgttaacgg cggatcgtaa
    25921 gtgacgaagt tgccgtaacg cgccaccata taatcgacaa tctctttttt acttttacct
    25981 tcctgcatca gttcatacac tttctgacgc aggtcggtgg caatcatcga gttggaatcg
    26041 gcaatgctgt tgttctggca tttcgggcag cgcagttctt cagtgagctg acggaactgt
    26101 tgttcctgtg cttcatcttt aaactgcaac acgtcgatgg tcgccagcgc tgagccggag
    26161 atcatcagca tcagcacgcc caataaaaac ctcattgtgc ggcctcctta ctgtatttct
    26221 cccacagcgg cttgatctct tcttcccaga cgcgaggatt gagatcgccc gcatggcgat
    26281 agcgaatgat accgttgccg tcaataagga aggtttctgg cgcgccatag acaccgagat
    26341 ccagccctaa catgccgtcg ccatcaaaca ggcttagcgc gtaaggattg cccagctctt
    26401 tcagccagct gattgccttc tggcgatcgt ctttatagtt catgccgacc acgcgaatgc
    26461 cctgcgctga aagctgattc agatattgat gttccgcacg gcaggtcgga caccaagtcg
    26521 cccagacgtt aagcagtact ggtttgccct gagtcagcac atccgcctga taaaactgcc
    26581 ccggattgtc cagtgattcg agacgaaact tcggcacagg cttgccaatg agcgccgatt
    26641 ccagattggt cggatcatcc ccttcggcat tacgcgccag ctgccacagc agcgccgcgg
    26701 caatcgccag gaagataatc aacggaatta acaatacttt gcgcttcata cggcctccgg
    26761 cgcagttttt tgcggactca cgcgcttacg atagcgagga tcaaacagac acagcagtcc
    26821 gcccaacgcc atcatcagcc cgcccgccca aatccagcga acaaatggtt tgtagtaaag
    26881 acgcacggcc cacgcgccgt tttccagctc ttcaccgagc gcggcgtaca ggtcacgcgt
    26941 gatgccgccg tcaattgccg cttcggtcat catcgacccg gcagtgttgt aataacgttt
    27001 ttccgcatac agcaccgttt ccggtttacc ctctcgcgtt acgccaatag tcgccacacc
    27061 gccgcgccag ttcgggccag tcacctcttt gacatcacgg aaggtgaagc gatattcatg
    27121 aatatcgacg ctatcgccgg acttcatgcg cacatcacgc tcaacgctat aattctggct
    27181 aaaggcaatg ccaacaattg tcactgccag ccccaggtga gccgccacca tcccccaata
    27241 actgaaggtg gttttcgtgc cgcgtgaaat acgcagcgca gcttccgcag ttgccagcac
    27301 cgcaatccag caggccattg ccaggccgag caccgtcatc gccacaactt tgctttcgaa
    27361 cagccacggc aacagcagag acagcaccag cgtagagatg aaggcgataa tcaataaatt
    27421 gcggatttta cgtgggcgat cgcgccccca gcgcaccaga ggaccgacac caagcagtag
    27481 cgcaaacggc accatcagcc aggtaaacat ggtgttgaag aacggttcgc caatcgaaat
    27541 actgcccagt ccaagttgct tgtgcaccag cggcagcagc gtccccagca acaccaccag
    27601 catcgcggcg accagcaaaa cgttgttcgc taacagcaaa gattcccgcg accacagcgc
    27661 attgtttacg cgtgagcgaa ctttgtgtcc acgcgcggca aacagcagca gcgaaccgcc
    27721 aatcaccagc accataaagg cgaggataaa cataccgcgc gccggatcag acgcaaacgc
    27781 gtgtaccgat accagcacgc cggaacgcac gaggaaagtc cccaacagac acaacgagaa
    27841 ggcactgatt gccagcagta atgtccacgc tttgaagctg gcgcgttgtt cagtgaccgc
    27901 cagtgagtgc atcagtgcag tccccaccag ccacggcata aacgaggcgt tttctaccgg
    27961 atcccagaac caccagccac cccagccgag ttcgtaatag gcccatgcgg aaccgagcac
    28021 aatgccgagc gttaggaaaa tccacgccgc cagcgtccac gggcgagtaa aacgcgcata
    28081 agtgctgtcc agacgcccgc tcagcaaaga agcaatggca aaagcaaacg ccaccgagaa
    28141 acccacgtac cccatataaa gcagaggcgg atggaagatc agccccggat cctgtaacag
    28201 cgggttaaga tcgcgccctt caatcgggaa gttcggcagc gtgcgtgaga acgggttaga
    28261 ggtaaagaga atgaacagca aaaagccgac gctgaccatc cccattatcg ccagtacacg
    28321 ggccacaata tccagcggaa tacgctgact aaaaatcgcc accgcaaagg tccagccgct
    28381 catcagcagc acccacagca gaagcgaacc ttcatgcgcg ccccaagtag ccgccacgcg
    28441 ataccacacc ggaagctggg tattggagtt gctggcaaca taggtgacgg taaagtcgtt
    28501 gaccacgaaa gcattgacca gcaccagaaa tgcgccagcc acagacataa acagcagcca
    28561 ggcaaacaag cgggaagacg ccatcatgcg cgcatctccg cgcgccacgc cccatagcgg
    28621 atacacggac agcagcagcg caatccctaa cgccaagcac agcagcccgt ttccaatttc
    28681 cggcatcatg atgctgggtc cttataaaca ctcgccgggc gacggtggtt agcttccatc
    28741 gctttctcaa cttctggcgg cgtgtagttt tcgtcgtgtt tcgccagcac ttctttcgcg
    28801 aggatatgat tgcctttttc cagctcgccc tgcaccacaa cgccctgccc ttcacggaac
    28861 agatccggca aaatgccttc gtaagagaca tccactgagc cttcagcatc gtaaatggtg
    28921 aaggtcactt tcagcgaatt gggatcgcgc tgcacgctac ccggcatcac catcccgcca
    28981 acgcgcagac gctgaccgac ttccggcatc tgctgagttt cacgcttgcc gtagagaatt
    29041 tcccccggcg tataaaagag atcgatattc gagcgcagcg catatagcac cagaccgata
    29101 gtcagcgcta gccctgccaa cacggcacag gcaatccaca agcggttttt acggcgaata
    29161 ttcatgcagc ctcctgctgt tgcgcagcac gtaaacgcgc ctcacgcgcc cgctgttgcg
    29221 ccacaccacg cagaattgcg cgatgttgca tcaccgagtg cacgaccaaa accaccagcg
    29281 gaataacggt catcaccacc gccagccaga caaaaaaggc gtaaccgccc attgcgaaaa
    29341 attcattcca ggaagcaaat gcaggggtca tttacggcct cttttcagta tcagttcact
    29401 cacccacgga cggcgttttt ccatcagcaa aatcaaatta cgcatccgca tcagcgtcag
    29461 cgtggcagac aggagcagga agccaaaaat cgaccagcgc agcggcgaac gcatcgccgg
    29521 atcgatactt tgctgcatcc gcgttgatcc ctgatgcagg gtgttccacc actccacgga
    29581 gtaatgaata atcggcagat tcaccacgcc aatcagcacc aggatacctg ccgcacggcc
    29641 cgccagacgg cggtcgtcga aggcgtgcca cagggcaatc acacccacat acaaaaacag
    29701 cagcaccagt tcagaagtca gacgtgcatc ccatacccac caggtgcccc acatcggttt
    29761 tccccatgca gagccggtaa ccagggcaat aaaggtaaac acggcaccaa tgggggccat
    29821 cgccgccacc gccaggttgg ccattttcat ctgccagaca aggccaataa acgccgccac
    29881 cgccattgat gcataaatgc ccatcgacca gatcgccgca ggcacatgca ggtagataat
    29941 gcggtagcta tttccctgct gataatcagc cggagcaaag ccgaatcccc agatccagcc
    30001 gacggtaagc acgaccacac tggcaattgc cagccacggt ataaaccagc cacagatttg
    30061 atacagccgt ggtgggatcg ccagttgatg cagtgttttc cacatagttt cgataccaga
    30121 ctcgaacaaa aatcagtaat ccagcgttat tgaatgctga ttcgtaacgc tgccgccgtc
    30181 gcaaaaggac ttaatgtcgc ggtgcctgcc agcaacgcgc ctaaaattgc cagataccca
    30241 tcaacgggca aatgcataga agccgcgtcc atcgcggcgg tggcaaagat gagtaatggg
    30301 atagtcagcg gtaacaccag tatgctgagc agcacaccac cgcgcttaag tcccactgtc
    30361 agcgccacgc ccggtgcacc gagaaagcca agcgtaggcg ttcccagcag cagcgtcagc
    30421 gccatcactt gccagccata aacatccatt cccagtagca ttgctaccag tggcgaaagg
    30481 atgagtaacg gcagaccggt taccatccag tgcgccatca ccttcgccag cacaacggcg
    30541 ggcaagggta acggcaacaa catcaattgt tcaagactgc cgtcctgcaa atcgtcacgg
    30601 aacagtcgtt ccagcgccag caaggatgaa agcagcgcag caacccagat aatgcccggt
    30661 gcaatacgcg ccagcagttg cggctccgga ccgatactga gcggaaaaag ggtaattaca
    30721 atcaggaaga accacagcgg gttggcgatt tcggcgctat ggcgaaacgc tacgcgcagt
    30781 tcaagacgga aaatacgcca gaacatcatg cggccctcgt ttgcgtcagt gaaatgcggc
    30841 gaattttact ttcagcaacg ttgagcggct ggtgggtagt cagaatcaca atccccccct
    30901 gctccgtatg ctgcgccata cgctgggtca gacgatcgac gccgttaacg tcaatcgcgg
    30961 taaaaggctc gtcgaggatc cataatgtgg cacgggtcaa ccacagacgc gctaaagcga
    31021 cgcggcgttg ttgcccggca gaaagttgat ttaccggaat atcttcgaat ccggcaagcc
    31081 cggcctgcgc caaggcttcc agacattgtg cggcatcgcc atcgcgatga taaaagtgca
    31141 gattttctaa cgccgtcagc cgggttttga tccccggctg atggcctatc cataacaggg
    31201 tttgatggta gctgtcgcgt acctgatgca agggctgccc ttgccagaga acctcgccag
    31261 cgtcagggcg agacaacccc gtcagcaaac ggagaagcgt tgtcttcccc gcgccgttgc
    31321 taccggtgat ttgtacccac tctcctgcgt tcagcgtaaa tgacaagcca ctaaataagg
    31381 ttcgttcatc ccgctcacaa agtaactctc tggcttcaag catacccacg caataaccct
    31441 gttaaaaacc tggctcgact tcacgcatat cgggcagctt gtgcgcgatc cctttatggc
    31501 aatcaataca ggtttgccca tctttcaccg cctggtcatg catcttcgcg gcaaccgatt
    31561 tctgggcggt tgtatccata tactcgaagt tgtgacagtt acggcactcc tgcgagttat
    31621 tgtccttcat gcgccgccac tcattctgtg ccatcgtcag acgatgagct tcaaatttct
    31681 gcggcgtgtc aataacgcca aaaattttac catacagctc tttacttgct ttgagcttgc
    31741 gtatcatctt cggcacaaac tcgtgcggaa cgtgacaatc cggacaggtc gcacggacgc
    31801 cgctacggtt gttgtagtgc acggaatcca tgtattcctg atacaccgtg ttgcgcattt
    31861 cgtggcagct aatgcagaac tcttcggtat tggctttttc catcccggtg ttaaagcccc
    31921 cccagaagac gatgccgcca acaaaaccga tcaacagcag cgtccccagc gccagatggc
    31981 tgggggtacg ccaccatttc cacaggcgct taatcagacc aggcttacgg tcagaatttc
    32041 ccataataac ctcttatttc ccgtaacctt ttgatggggt aaaggtattc cccacgattg
    32101 gcgcggtatc ggcctgcggt acgtgacatt gcagacagaa ataacgacgc ggagccactt
    32161 ccgcgcccac tttgccgtcg ctgtccataa agtgagtagg actgatacgc ggcgcgccag
    32221 tggtgcgata gctttcgaca ccgtggcact gcaagcagcg attggtattg gtcgttacct
    32281 gataaccttc aacgctatgc gggatcattg gcggctgatt cacatagttc agcggcatcc
    32341 gatcctgctc ttttggcatc cgaatggccc cttcctgcgt cccggatact tccggcgact
    32401 ggctaaaatc gacgccatta gccgcccaaa ccgcgccgct taccaccagg gccagcatcg
    32461 ccgtccattg acacagcgct ttcttcaggt catggctttt catgatttcg ctcccgaact
    32521 ccatcgtgta gttattgtaa atacatcctc agaacagaca tccacgcagc gaccgcacgt
    32581 catgcaatcg cggctggtga cctgcaccgg gctttgttca tccagcaccg gggcacgtag
    32641 cacatgcggt tccgggcaaa catgaaaaca atccatacag cggttacatt tctgccgatc
    32701 gctggccgca acggtaatca cacctttgct acccagcacg ccatacagcg cgccaaccgg
    32761 acaaatgtgc ccgcaccagc cgtgttcaac gaccagtaga tcaaataaaa acagcgcgag
    32821 aataagcagc gcgccgctgc cgaagcccat taccaggcta cgccccatca gagaaacggg
    32881 gttgatccat tcccaaatga gcgtgccagt taaggctgat cccaccagaa tgaccaccaa
    32941 cagcacgtag cgaatgtggc gagggatcgt cgcagactga ttgaggtcaa agcgcctgcg
    33001 taaccagttc gctaagtcgg taatcgggtt cagcgggcag acccagctgc aaaataatcg
    33061 ctttcccgcc agggcgtaga gcacggtgat aatcaccgca ccggtcaacg ccaccgtggc
    33121 aggcagatga ccactggcaa gactttgcag cgtcatcagc ggatcggtca gcggaacggt
    33181 gtcgaataac aggctactgc tgtagttgcc gtgcaagatc cacacaccaa accatggacc
    33241 gctcaaaaac atccccagca cgaagaactg acaaaggcga cgtaacacca gccaacggtg
    33301 actgcgccac cagccttttt tctccagcgc ctcgcgcccg gcgtcacgtt tacgatttgc
    33361 cattgttccc ctccagccag ccgaagcggt aatggtgacc taactcccct ttcgccagtg
    33421 acagcggtaa caccttgatt gccggttgtt ccagcacgca tactttttcg cacttaccgc
    33481 aaccggtaca ggcgtcgctg tgaaccgtcg gcagaaagcg ggcgtgctta ccggtacgcg
    33541 tgttgcgctc cagctccaag gtgatggcct catcaatttt cgggcattcg cgataacaaa
    33601 catcgcagcg cagcccctga aagttgagac agttttcctg gtccaccagt accgccagcc
    33661 ccatccgcgc gtcgtcgatc gattcaatct cacgatccag cgcaccgctt gggcacactt
    33721 tggcgcacgg aatgtcctca cacatttcgc aaggaatatc ccgtgcgaca aaatatggcg
    33781 tgcccgccga cagaccagag gccagcgtcg ccagttttaa ggtgtcgtaa gggcaagcct
    33841 gaacacactg accgcaacgc acacaggcac tggcaaaggc gttctcgttt atggctccgg
    33901 gcgggcgcaa ccgcacgcca gatgcgcgtg cggtttgctg ttgtaacccc agcgccacac
    33961 ccacggcagc cagcccgcct gctgtgcgaa cgacatcgcg cagaaagcgg cggcgaccat
    34021 tttgaggttt cgctgaccgg gacataatgg cttacacctt ctccagtttg accgcgcact
    34081 tcttgaagtc cgtctctttc gagagcggat cggtcgcatc cagcgtcagt ttgttaacca
    34141 gctgtgcggc gtcgaagaac ggcatgtaca ccaggccctg tggcggacgg ttacgaccgc
    34201 gcgtttcaac aatcgagatc acttcgccac ggcgagaaac cactttcact ttgtcgccac
    34261 ggcgcagatc gcgcgctttc gcatccagcg ggtgaataaa caagaccgct tccgggaagg
    34321 cgcggtgcag ttccggcaca cggcgagtca tactgccggt gtgccagtgc tccagaacgc
    34381 gtccggtaga gagccacagg tcgtactctt catccggtgc ttccgccgcc ggttcgaacg
    34441 gcagtgcgaa gatcaccgct ttgccatccg gtttaccgta gaacttatag ccttcgcccg
    34501 ctttcacgta cgggtcgtta ccttcgctgt aacgccactg tgtttcttta ccattcacca
    34561 ccggccagcg cagaccgcgc gctttgtggt agtcatcgaa cggtgccaga tcgtgaccgt
    34621 gaccgcgacc aaaccatgcg tactcttcga acagcccttt ttgcagatag aagcccagct
    34681 cgcgggattc atcgttcagc tgatcttccg ccagttcgga taccgggaat ttgctcactt
    34741 cgggggtggc atacagaact tcgtacagcg ttttgccacg cagttccggt ttcttcgcca
    34801 gcagctcttc cggccatact tcttcagttt tgaagcggcg ggagaactgg actaactgcc
    34861 agagatccga tttcgcttcg cctggcgcct gtacctgctg acgccagaac tgagtacggc
    34921 gttcggcgtt accgtaagcg ccctctttct ctacccacat tgcggtcggc aggatcaagt
    34981 cggcggccag cgcactgact gtcggatacg gatcggagac gatgatgaag ttgcgcggat
    35041 cgcgccagcc cggcatacgc tcttcattga tgttcggccc ggcctgcatg ttgttggtac
    35101 acatggtcca gtaaacattc agcttgccgt ctttcagcgc acggtcttgt gccaccgcat
    35161 gaagaccgat tttcgccgga atggtgccgc tcgggatatt ccacttcttc tcacagatat
    35221 cacgatgttt ctcgttagtt accaccatgt ccgcaggcag acggtgagca aaggtgccca
    35281 cttcacgcgc agtaccacac gcagaaggtt gcccggtcag ggagaacgga ccgcaacccg
    35341 gctgggaaat tttgccggtc agcaggtgca ggttgtagac caggttgtta gcccacacac
    35401 cacgggtatg ctggttgaag cccatcgtcc agtaggagat gactttcttg ttcggatcgg
    35461 catacagctg cgccagttgt tctaactggt ctttcggcac gccggtcatt tcagcggttt
    35521 tttccagcgt atactcagca acgaaggctt tgtaatcttc aaagctcatc ggttcggagg
    35581 cgtcagaacc cggattcttc gctgcttttt ccagcggatg ggtcggacgt aaaccgtagc
    35641 cgatgtccgt cgcccctttg cgcaggttaa cgtgcttgct gaagaagtcc tgatttatcg
    35701 cattgttttg aatgatatag ttggcgatgt agttcaggat caccaggtca gattgcggcg
    35761 taaagatgat gccgttatcc gccagctcga agctacgatg ctggtaggta gaaagcaccg
    35821 ccacggtgac gttctggtta gagagacgac tgttagtgat gcgtgaccag aggatcgggt
    35881 gcatctccgc catgttagcg ccccacagca caaacgcgtc agcctgctcg atgtcgtcat
    35941 agcagcccat cggctcatcc ataccaaagg tacgcataaa gccaactact gccgacgcca
    36001 tacagtgacg tgcgttcggg tcgatgttgt tcgaacggaa gcccgctttg aacagcttgg
    36061 acgcggcata accttcccag atagtccact gaccagaacc gaacataccg atcgattccg
    36121 gccctttttc tttcagggcg gttttgaatt tctcttccat cacatcgaag gcctgatccc
    36181 aggtgattgg ggtaaattcg ccttctttgt catatttacc gtttttcata cgcagcagcg
    36241 gctgcgtcaa acggtcttta ccgtacatga tcttgggcag gaaatagccc ttaatgcagt
    36301 tcaggccacg gttaaccggt gcgtccgggt cgccctgaca ggccaccaca cgcccctgct
    36361 gcgttccgac cagaacgccg caaccagtac cgcagaaacg gcacggcgct ttatcccatt
    36421 taatggcttc ctgctgacca acaacggcgc gggcaacgcc cggcacgctg agaccggcag
    36481 ccgccgcagc ggccgcaacg gcgttagctt tcataaagct acgacgactg agtttcatgg
    36541 tgtttcctca ccttgctctt cctgctggtg ataaaccagc gacaccgcca gcacgccctc
    36601 tacgttgcgt actgactcaa tggtttggat cagcgtttcg ctgtcttctg cttccaccac
    36661 cacaatcaac tgaccgctcg gcgcgtcgct gacagcaact tcacagccgg gaaaggcgtt
    36721 cagttgggtg ctgatgtctg aaattcgttc gcttttggcc tgcacgacca ggctgcaaac
    36781 ttgccagtta gtgtgcatgg agatactccg cagttatggc tgataccgga cagctggctg
    36841 cacatgctcc acatccgttg caaagttggc tattaagttg cgcctggtag atcccggaga
    36901 gcgtcgggcg aaagataatt gccattggct cacagctatc ctgacagcgg cggcattcaa
    36961 ctgactgata cgccaggcac gcgtccccga tggtgaactg caaatcccag gccctggtgt
    37021 ggcgcggaga aaatagcgat tcggggcaag cctgcgcgca ggcgtaacag aagctgcact
    37081 cgttattttt gaaattaacg ctcggatagc cacccgtgcc gcgttgcaga atgttgtttt
    37141 cacaagcatt aatacaggcg tcgcagcggg tgcaatgggt cagaaaatga gattcatcac
    37201 cggaccaggg cggacggata ccgttactgg ctttgcgcca gcaaccagtg agtatgcccc
    37261 gacgggatgc atcaatcttc acattgacct tccatcatta acgcgctctg aaaattgaga
    37321 gcgaccaaat agaccgcata attaataagc catttttata gccgctaaga tattaaagga
    37381 tgtgtcaaag atgcataccc cgatcggggt aaaacctgta gcaggatcaa aagagtggcg
    37441 ggaagcgtgg caaaaacggg cttttgctca catttcaaat ggttataaac atatttatat
    37501 agcgattaat tcaccagaga tatttctgct ggtttgctcc ctcatcagaa tttaacacta
    37561 aaagagcagg taaaattgtc tgaatgttct ttaagttatt cataaagcaa attaataaat
    37621 ctgatgaata tgttaacctt cagcgacatc atcggtgaaa acctataaat gaagaaggaa
    37681 agcaaaaaaa tgaagaccat tctacctgaa gtattgtttg ccgctttcgc taccacttcc
    37741 gcctgggcgg cagaaagcgt ccagccactg gaaaaaatcg cgccttatcc acaagctgaa
    37801 acaggcatga agcgtcaggt gattcagtta accccgcaag aagatgaatc taccctgaaa
    37861 gtagaactgt taatcggtca gacgctggaa gtcgattgca atttgcatcg tctcggcggg
    37921 aagctggaaa gcaaaacgct ggaaggctgg ggctatgatt attatgtctt tgataaagtt
    37981 agctcgccgg tttccacgat gatggcctgc ccggatggca agaaagagaa gaaatttgtc
    38041 accgcgtatc tgggcgatgc tggaatgctg cgttacaaca gcaagctgcc gatcgtggtg
    38101 tatacgccag acaatgtaga tgtgaaatac cgcgtctgga aggcggaaga gaaaattgac
    38161 aacgcggaag ttcgctaaac tgttgtgaag tacggcaccc cgttggttgg ataaggcggt
    38221 tacgccgcat ccaacatcta acgcccgagc cggttgcctg atgcgacgct ggcgcgtctt
    38281 atcagtatac gccacatcat tacaacgcaa tatccgccac ttctttttga acgggttgcg
    38341 gtttcaactg cggtttcggc gtactatctg ctgcttgcgg tttgtcgtat ttcagcccca
    38401 gcacttcgct ggtgtactgc aactcgcgtt ctgttgccgc tacatcaccg ttcagcttgc
    38461 gtccataaga cggaacgatc gctttcaacg tagcctgcca ttgcgggctg gaaacgcgat
    38521 cgccaaatac tttttccagt agattcaaca taatcggcgc ggcggttgac gcccctggcg
    38581 atgcccccag cagcgccgca atggtccctt gctggtcact gaccacttcg gtacccagac
    38641 gcagtacgcc acctttctcg gcatcacgct tgataatctg cacacgctgc cccgcttgcc
    38701 acaaacgcca gtcctctttt ttcgcttgcg gatagtactc tttcaacgct tcaaaacgat
    38761 cctcttcact caacatcacc tgactcacca gatatttcac cagatcgaaa ttatccagcc
    38821 cgacgtgcat catcggcatc acgttagagg tggtggtgga actcatcaga tcccacaatg
    38881 aaccgttttt gaggaatttg gttgagaagg tggcaaatgg cccaaacagc actacgcgtt
    38941 taccgtccag aacacgggta tcgatatgcg gaaccgacat cggtggtgcg ccaacggatg
    39001 ctttaccgta aactttcgcc agatggcgat taaccacgtc tggattttcc gaaacaagga
    39061 actgtccgcc caccgggaaa ccggcgtagt ctttcgcttc cggaatcccc gattcctgta
    39121 acagcttcag cgccgcaccg cccgcgccga taaagacaaa tttggcgcgg atgttctgtg
    39181 cagtgccgtt tttcagatcg gcaacggtaa cggtccaggt gttatcgtca ttacgcttta
    39241 gggcgcggac ttcgctgctg agttgcagcg agaagttaga tttcttctgt aaggaagcaa
    39301 ttaactggcg ggtgatctca ccatagttca catcagtgcc aatttctgtt gcggatcgcg
    39361 cccttccatc actaacggtg cccactcttt gatctgcgcg tgatcttcag agtaacgcat
    39421 accgcgaaac agcgagcttt gttgcaacgc ggcgtaacgg gcgcgcagga aattaacgtt
    39481 atcctcgccc cagacaaagc tcatatgcgg aacggtattg ataaatgaac gcggagtacg
    39541 cagcacgccg cgctcgacct ggtgcgccca gaactggcgg gaaatctgaa atgcttcgtt
    39601 aatggcgact gctttttcaa tactgatgct gccatcggcg ttttgcgggg tgtagttcag
    39661 ttccatcagt gcagaatgcc cggttccggc gttgttctag ccgttcgaac tctcctgcgc
    39721 gcccccctcc aggcgctcca ccatggtcat cgaccattca ggctccagct cgcgtcaata
    39781 ggtccccaac gtggcgctca taatgccgcc gccaatcaac agtacatcag tttcctgctc
    39841 ctcggacgct ttcgcttttg ccgccatcga aacggcatta agccccacgg ccatcgagaa
    39901 gagcatggca gtcacttttt tcatcttgtt aatgccttac ttttagtcgc tttattgcag
    39961 gtgagatttg cgcggcatca acggtaacac ttaagtaaca acatttaaat aatgtttata
    40021 aattatattc taattttaga aacattataa tttcgttgat taattatagg gttattagaa
    40081 atgaagggat ttttagcata ttgacctgac ggcagcagtc cgccaggtca gcgtcgtaat
    40141 attatgccgt ccgggcaacg gcatcacgcg aagcggcatc gcgctcttcg cccgtcagct
    40201 cgctaagttg cccattgcgc atttccagca ggcggtcggc gtggataaag taatgatcat
    40261 catgactgat agcgaaaata gttttaccca tctcctgcat cagcggcagc aacacctgat
    40321 aaaactcacg acggaagtgt ggatcctgat ccaccgccca ttcatccagc aggataatat
    40381 cgcgttcttc tgccagcgcc agcaacagcg ccacgcgttt tttctgccct tttgataact
    40441 tcaggttaac aatacgcccg ttgcttaact caagcttatg agccattttc agctgcgcca
    40501 gccacttctc aaccagttgc gggttagcgg gttgcccctc cggccccagc agttgatcaa
    40561 acagccagac atcggtaaac actgccgaaa acagtttgcg ataatcttcc ggttgttcgc
    40621 cgctgacagg tttgccatcc agcaagattt cgccgctttg tggctgatac aagcccgtca
    40681 acaacatcgc cagcgtcgat tttccgctac cgttaccgcc aatcagaaac agcagctcgc
    40741 cacgtttgat ggtgagatta atcggaccaa cggaaaacgc gttatcctga taagcaaacg
    40801 tcacgttacg cagctccagc gtttgccagt tgggaaacgc ctgcgggcgc ggaaactcag
    40861 ctttgaaagg cgcgagcgcg aatttgttca gcttgttaaa cgccacctgc gccgtcagca
    40921 gcgtcggcaa tgcgccaacc gccgaaagca gcggcgtacg caggaataac agcgtcaacg
    40981 aataggtcgc agcaacgttg gtatcagccc aaccgagact gttcgccatc cagaacacca
    41041 ggccgatagc gcccagcatc atgatgtttg accagttcac ggcactaaga tggaaggtgt
    41101 ctgcgcgaat aatatgatgg cgatactctt gcgcatcggg gatgtagagg ttgttaaaca
    41161 catactcggc gcgttcccgg ttcagagtca gctctttgcg cccttcaagt acagtttgaa
    41221 aatccgtgta cagcttgtct tcggtttcac gtagggtcgc catatgtttg tacacccgcg
    41281 ccaccagcac aaaaccgccc cagatggtga tcgccatcca gatagccgtt accagcaaca
    41341 ttttgcccga cagcatccac agatacgccg atgaaccgat agtgagaatg atcccctgcc
    41401 ccagttccgg cagacgcaca aaagcaatgg tgatattgcg cacatcgctg gttaatcccg
    41461 ccagcaacga ggcgctaccg agttgttcaa tgcgctcgac gtgggtatcc agaatccgct
    41521 tgataaattc actacgcagt cggtaaacga agtgatgccc caaagtggtg agcgccagtt
    41581 gcgatccgag agtgactgcc atcaacagca gcaataatcc cagaaactcc ggtaacacca
    41641 gcagactggt atccgccgtt tcgataaggc gctgattgat aaaagcaatt aagccaatgc
    41701 ctaatgccgc actggcgagg cttagcgcca tcacactgat aaatggccag cgatactgcc
    41761 gccagacaag tacaagaagt tccatgcaga aaacccggac aatgaattac agcccgcagt
    41821 ttaaacatct tcgcgcgcac agcaataata attcttattt ttattctttt ttacctgcct
    41881 gacggaatgt caggttgtag cggcagtcgg tggtgagtgg atgaaacccc gctttcaacg
    41941 gttgaatacc gtgataaaac agccgcgatt caccgcccca taccaccaca tcgccatgtt
    42001 ccaacaacaa acgtttgagc ggatcattac gtttcaggcc accaaattga aaaatcgcgg
    42061 gtaagcccag agaaacagaa acaattggcg cgcgcagatc cggttcgtct ttatcctgat
    42121 gcagcgacag tttcgcgcca ggagcgtagc ggttgataag acaagcatct ggctggaaat
    42181 ctggatagcc cgccgccgta gccgcacgtt gacataaatc atgaaagctt tgtggcatag
    42241 cgggccacgg tttatttgtt tgcggatcaa tgggcgaata gagataacct tgccgatggg
    42301 tcgtccagcc cagatgccca cagttggtca tcgccaccga catggtatat ccgcctggag
    42361 cgaccatctg gcgaaacggc gactggctgg caacgtcatt aatatcgcgg attagttgct
    42421 ccgcagcatt aaaagcaaaa cgccgtaaaa ttaccgcacc agccgccagt ggctcttgcc
    42481 acggttcagc atcggcaaac agatccaaca ttacctctcc tcattttcag cttcgcggcg
    42541 cagcagttgc gctttacgcg acacgcccca gcggtaaccg gaaagtgtgc catcaccacg
    42601 gaccacccga tgacagggta ttacgatagc cagcttgttg gcggcacagg cgctggcaac
    42661 ggcccgtacc gctttcggtt tgccgatggc gttagccagt tgctgataac tgacggtttc
    42721 accacaaggt atcgtgcgca gtgcctgcca gacttgttgc tgaaaagcag tgccgcgaat
    42781 atccagcggt aacgtcagcg gcgtatcgcg ttgattgagg ctggcgatca cttcacgcac
    42841 atgttgctga aacgtcagat cggcaggcgc gttgtcggca gcgggaaaca tctgctgcaa
    42901 ctcgctgatt aatgtcgcgt catcatcgcc cagcaatatc gcgcaaatcc cccgctcgct
    42961 ttctgccacc aggcaacgac ccagctcaca atcagccagc gcgtaacgca ccgccagatt
    43021 ttcgccaccg tgacggaatt gtttagccgt catgcccagc gtttcatcgg ctttgcggta
    43081 gtaactgctg ctgtcgggga atccggcgtt aagaatagac gtcgtcacgc tctccccttt
    43141 cgccagcgat tcgcgcaaac ggcgagcgcg ccaggcctgt tgccaggctt taggcgtcat
    43201 tccggtagta gctttaaaca accgatgtag atgaaatgga ctcatcgcca cctggtcggc
    43261 taaggcttcc agcgttacag gcgtttcctg ttccagcagt cgacacgcgt gggtgatttt
    43321 atccaaccga tgttgccggg gattggcttt gtctggctga caacgtttgc aggggcgaaa
    43381 tccggcggcg agtgcctcgc tggcatttgc gtagaaggag acattttccc gcaaggcatg
    43441 tctggcgcgg caagaaggac ggcaaaagat acccgtggtg cgcacggcga aaacgaattc
    43501 gccgtcggca ttcgggtcgc gggctaagac agattgccag cgttgatcgt cagttaagca
    43561 tgtggcgttt ttcataatca gctccctggt taaggatagc ctttagtctg cccggtcacc
    43621 atcacgcaaa aaccaacaat cttgcgcttt aatttttttc gctgacaagg aagcttttaa
    43681 actgtggtga catccaggtt ttaaagctat cgccttcttt ggtgatcata taaaccgcca
    43741 gcccttcccg gcgaacaacc tctttggctt tctccggccc gagtaccatc aagccagtat
    43801 cccaggcatc ggcttccagc gccgtcggag caatcaccgt cacggatacc agattgtgtt
    43861 cgatgggacg cccggtttgc ggatcgataa catgggaaag acgtttgccg tccagttcgt
    43921 aatagttacg gtagctgcca gaggtgctaa tcccatgacc gttgatatcc accacagcct
    43981 gaaccgcgtt ttctttatcg gttggttttt gaatcgctac ccgccacggc tggccttcac
    44041 cgttcatacc acggctgttc agcgcgccgc ccaccgacac cagatagcgg gaaatccctt
    44101 cctgctccat caagcgtgcc aggtgatccg ccgcataacc ttcaccgacg gtggagagat
    44161 cgacatataa atccggcagg tctttttgca gatattgctg atgcgactga ttgatgaccg
    44221 tcaggtgctg taagccggtt ttggctttca tcgcatcgat ctgttcctgg ctcggaattt
    44281 gaaccggctg ttgttccggg ccaaagcccc acagattcac cagcggcccg acggtaatat
    44341 ccatcgcacc atcggtcctt gccccaatgc gcagcgaggt ggtgacgata tcggccatcg
    44401 cttcacttac cggccacggc gacaaacttt gcgagtcgtt aaagcgcatc agcgcggaat
    44461 cttttttata ggtcgaaagc agctgatcgt cggcgtccag ctgggtctga atcttttctt
    44521 taagttcggc actgcgtttg gcatcaatgc ccgggatgct ggcacgccag aaggtaccca
    44581 tggttttgcc ttcaagaacg gtaacttcag tagcgtgggt tttggcgggt tgtggttttt
    44641 gatcgcaacc aacaaagaag agcgcggcaa ccagcagtgc cacgcgggta aagcttattt
    44701 ccatacgtga ttatcctcat gcgaacggtc gcaagagtac accaaaaaac tgtgtttgta
    44761 cgctgaaaac aatgaaaaaa gggcccgcag gccctttgtt cgatatccat cgagattaga
    44821 actggtaaac cagacccaga gctacgatgt tatcagtgtt gatgccagcg tcacgagtga
    44881 actggttgtc gtccagcagg ttgattttgt agtcaacgta ggtggacatg tttttgttga
    44941 agtagtaggt cgcaccaaca tcaacatatt tcaggatatc ttcgtcgtcg tagttacgac
    45001 cattgatgac acccaggttt ttacctttag actgcaggta agccagggac ggacgcagac
    45061 cgaagtcgaa ctggtactga gcaacagctt cgaagttctg tgctttgttc gcccaaccca
    45121 agttgcctac gcgagttgcg ttgtaggtct gggtgtactg agcagctagg tagatgttgt
    45181 tcgcgtcgta tttcagacca ccggtgtagg tttcagcgcg gtcgccatta ccgatgtaac
    45241 gctcatcata acggttcaga ccgaagttct ggtcatcagt acgtttggag ctggaaactg
    45301 cagcaccgat accgaagcct tcgtaatcat aagtaataga accgccaacg ccgtcaccgt
    45361 tctgacgcag agcttcacga ccgttgttgg tcatgccttc accttccggg ctaccgtttt
    45421 tgccctgata ctgaacagcg aagttcagac cgtcaaccag accaaagaag tcggtgctac
    45481 ggtaggttgc gaagccgtta ccacgctgct gcatgaagtt gtcagaaccg taagtgtcac
    45541 caccgaattc tggcagtacg tcggtccagg aagttacgtc gtaaactacg ccgtagttac
    45601 gaccgtagtc gaaagaacct acatcctgga atttcagacc tgcgaatgcc acacgggtcc
    45661 aggagttgtt ttcgttttca gcgctgttgc cctggatctg atattcccac tggccgtaac
    45721 cggtcagctg gtcagtaacc tgagtttcac ctttgaagcc aagacgcatg taggtctggt
    45781 cgccatctac agacttgtcg tcagagaaat agtgcaggcc gtctacttta ccgtacagat
    45841 ctaatttgtt gccgtctttg ttgtaaactt cagcagcgtt tgctgcgcct gctaccagca
    45901 gagctgggac caggagggac agtactttaa ctttcatgtt attaaccctc tgttatatgc
    45961 ctttatttgc ttttttatgc cactgcatac tgattaaccc tcattaatca gtcggcaagt
    46021 ccattctccc caaaaatgca gaataatcca acacgaatat gatactaaaa cttttaagat
    46081 gtttcattta tcgctataga tgtttcaaaa tgtaaataca agggaacttt ttaagattat
    46141 tgcggaatgg cgaaataagc acctaacatc aagcaataat aattcaaggt taaaatcaat
    46201 aacttattct taagtatttg acagcactga atgtcaaaac aaaaccttca ctcgcaacta
    46261 gaataactcc cgctatcatc attaacttta tttattaccg tcattcattt ctgaatgtct
    46321 gtttacccct atttcaaccg gatgcctcgc attcggtttt tttacccttc tttacacact
    46381 tttcattatt ctgtgctacc acagaaaaac tataacgctt gttaactatt tcacaaataa
    46441 ttaacatcag cataatttcc agcaatcttt gtttatttgc aattaatttc gttgggcttt
    46501 ttgtaggtta tttgtacagc aaaatggcgc ttgtacatct atttcccccc atacaggatg
    46561 ataaatatca cgggagaata gagaatcatc aatcaggtaa gagtctggaa tttcacactg
    46621 taccctttat actgccctat cacttcgcga agttttaaca ggtcataaac acgaatgcgt
    46681 cagaaagaga caacggccac gacccgcttt tcactcctac cggggagcat tacccgcttc
    46741 tttttactgt tgatcattgt gttactggtg acgatggggg taatggtaca aagtgccgtt
    46801 aacgcttggc tgaaagataa aagttaccag attgtcgaca ttacccacgc aatccaaaag
    46861 cgcgtcgata cctggcgtta cgtgaccagg cagatctacg acaacattgc cgcgacgacc
    46921 tccccctcct ccggcgaagg tttacaagag acgcgcctga aacaggatgt ctactatctg
    46981 gagaaaccac gccgcaaaac ggaagcgtta atctttggct ctcacgacaa ctcaacgctt
    47041 gagatgactc agcggatgtc cacttatctg gatacattgt ggggtgcaga aaatgtaccg
    47101 tggtcgatgt attacctgaa tggtcaggat aacagtctgg tgctgatctc aaccctaccc
    47161 cttaaagatc tcacctccgg atttaaagaa tcgaccgtca gtgacattgt tgattcacgt
    47221 cgtgcagaaa tgttacaaca ggccaacgcc ctcgatgaac gcgaaagttt ttctaacatg
    47281 cgccgcctag cctggcagaa cggtcattac tttaccttgc gtaccacgtt caaccagccg
    47341 ggacatctgg caacggtcgt ggcttttgat ctgccgatta atgatttgat cccaccgggt
    47401 atgccgctgg acagtttccg ccttgagcca gacgcgacgg caacgggaaa caatgataat
    47461 gagaaagaag ggacggatag cgtcagtatc cactttaaca gtacgaagat tgaaatctcc
    47521 tcggcactca actctaccga tatgcgcctg gtctggcagg ttccttatgg caccttattg
    47581 ctggatacgt tgcaaaacat tctgctgcca ctgctgctga acatcggttt gctggcgctg
    47641 gcgttatttg gctataccac attccgccat ttctccagcc gcagtacaga aagcgtcccc
    47701 agcacggcgg tcaataacga attgcgcatt ttacgggcaa tcaatgaaga gatagtctca
    47761 ctgttgccgc tcggcctgct ggttcacgat caggaatcga accgcactgt cataagtaac
    47821 aaaattgccg atcatttgct gccgcatctg aatctgcaaa acatcaccac catggcggaa
    47881 cagcatcagg ggattattca ggcgacgatc aataacgagc tgtatgagat ccgcatgttc
    47941 cgcagccagg tcgcgccgcg cacacaaatt ttcattattc gcgatcagga tcgcgaagtg
    48001 ctggtaaaca agaaactcaa gcaggcgcag cgtctgtatg agaaaaacca gcaggggcgg
    48061 atgaccttta tgaaaaacat tggcgatgcg ctgaaagaac ccgcacagtc cctggcggag
    48121 agtgcggcta aactcaacgc cccggaaagc aaacaactgg cgaatcaggc agatgtgctg
    48181 gtgcggctgg tcgaagaaat acagttagcg aacatgcttg cggatgatag ctggaaaagt
    48241 gagacggtgc tgttctccgt gcaggattta attgatgaag ttgtgccttc agtgttgcct
    48301 gccatcaagc gtaaaggtct gcaactgctg attaacaatc atctgaaagc acacgatatg
    48361 cgccgcggcg atcgcgatgc cttacgacgt attttgctgc tactgatgca atatgccgtg
    48421 acctcaacgc aattgggaaa aatcaccctt gaggttgatc aggatgagtc ctctgaagac
    48481 cgcctgacat tccgcattct ggacacggga gaaggcgtaa gtattcatga aatggataat
    48541 ttgcacttcc cgtttatcaa ccagacccaa aacgatcgtt atggaaaggc ggacccgctg
    48601 gcattctggc tgagcgatca actggcacgt aaactgggcg gtcatttaaa catcaaaacg
    48661 cgggatgggc ttggtacacg ctactctgtg catatcaaaa tgctcgccgc tgacccggaa
    48721 gttgaagagg aagaagagcg tttactggat gatgtctgcg taatggtgga tgttacttcg
    48781 gcagaaattc ggaatattgt cactcgcctg ttagaaaatt ggggtgcaac ctgtatcaca
    48841 cccgatgaaa gattaattag tcaagattat gatatctttt taacggataa tccgtctaat
    48901 cttactgcct ctggcttgct tttaagcgat gatgagtctg gcgtacgaga aattgggcct
    48961 ggtcaattgt gcgtcaactt caatatgagc aacgctatgc aggaagcggt cttacaatta
    49021 attgaagtgc aactggcaca ggaagaggtg acagaatcgc ctctgggcgg agatgaaaat
    49081 gcgcaactcc atgccagcgg ctattatgcg ctctttgtag acacagtacc ggatgatgtt
    49141 aagaggctgt atactgaagc agcaaccagt gactttgctg cgttagccca aacggctcat
    49201 cgtcttaaag gcgtatttgc catgctaaat ctggtacccg gcaagcagtt atgtgaaacg
    49261 ctggaacatc tgattcgtga gaaggatgtt ccaggaatag aaaaatacat cagcgacatt
    49321 gacagttatg tcaagagctt gctgtagcaa ggtagcctat tacatgaaca atatgaacgt
    49381 aattattgcc gatgaccatc cgatagtctt gttcggtatt cgcaaatcac ttgagcaaat
    49441 tgagtgggtg aatgttgtcg gcgaatttga agactctaca gcactgatca acaacctgcc
    49501 gaaactggat gcgcatgtgt tgattaccga tctctccatg cctggcgata agtacggcga
    49561 tggcattacc ttaatcaagt acatcaagcg ccatttccca agcctgtcga tcattgttct
    49621 gactatgaac aacaacccgg cgattcttag tgcggtattg gatctggata tcgaagggat
    49681 cgtgctgaaa caaggtgcac cgaccgatct gccgaaagct ctcgccgcgc tgcagaaagg
    49741 gaagaaattt accccggaaa gcgtttctcg cctgttggaa aaaatcagtg ctggtggtta
    49801 cggtgacaag cgtctctcgc caaaagagag tgaagttctg cgcctgtttg cggaaggctt
    49861 cctagtgacc gagatcgcta aaaagctgaa ccgcagtatt aaaaccatca gtagccagaa
    49921 gaaatctgcg atgatgaagc tgggcgtcga gaacgatatc gccctgctga attatctctc
    49981 ttctgtgacc ttaagtccgg cagataaaga ctaatcatct gtaggccaga taagacgcgt
    50041 tagtgtctta tctggcattt gcaccgattg ccggatgcgg cgtaaacgcc ttatccggcc
    50101 tacgattccc attatttcaa cagattacat taaagtaggc cagataagac gcgtcagtgt
    50161 cttatctggc atttgcactg aatgccggat gcggcgtaaa cgccttatcc gtcctacgaa
    50221 tcccgcgatt tcctgaccct ctcggcatat accgtcagcg tctgttttat cacatccagc
    50281 gttaccggct tcgagaggca gctgtccata ccggactcca gacaccgctg cttctcttca
    50341 gccaacgcat tagcagttac tccgattacc ggcaacgtca gtcccaactg acgaatgcgt
    50401 tgcgtcaagc ggtaaccatc catatttggc atgttgacgt cgctaagcac gatatcaata
    50461 tgattcttac taagtacatt aagcgcatcg acgccatcat tcgcggtttt acattgatag
    50521 cccaacgatc ccaactgatc tgccagcaga cgtcggttaa tcggatgatc atccacgacc
    50581 agaatcatca tatcgtcgtt atcgctgacc gctttgtccg tcgacggcag agcgttagca
    50641 ggatcgtcgc tctccatctc gatcaaataa atacgcgcca acaatgccgg tagctcatgc
    50701 ggagcagcca cactgtgtac ccactcccct ggcgctttct ccagcggaat accaatatgg
    50761 cgacgacaga aggtcactac cgctctgccc tgccattttt tactcactac ctcgtcagtg
    50821 attaacacat cttcgggagt cggttcctgc ccttcgtatg ttgtaacgac gatgccgctg
    50881 cgctgcaaac tggtttccag gaactgacag agcgacgcat tgcggaccgc cagccagcag
    50941 cgtttaccac tcaacccttc cacgcctttt ttctgcgggt actgagcgcc gtacaacgga
    51001 atacgcacgg taaactggct gcccattccc ggttctgaat ctaccgagat atcgccgtcc
    51061 atcatgctga tcagtttttc acaaatcgcc agacccagac cggtcccctg gaagtttcgc
    51121 tgtacgcccg ttccaacctg gaagaaggga tcaaacaagc gcaccacttc tttcgccgga
    51181 atccccacgc cggtatcgcg aacacggata gagagataat cgccatcagc gcgaacatgc
    51241 aaaactatac agccggtatc ggtgaatttt atggcgttac tcaacaggtt ggagatgacc
    51301 tgctgtaaac gcatcgggtc gccatttaag gccactggca catccggttc aataaagcag
    51361 tacaagccta actgcttgcg taccaccagc ggtaaatagt tggcggtgat gtggttcatc
    51421 acttcacgcg gtgaaaactc acgcggttcg atcttcaact gttccgattc aatcttcgag
    51481 aaatcgagaa tatcgctgat aattttcaac aacaggctgg aagagttgtt cattgccgtc
    51541 accagacgat cgacgccttt cggtaactct ttggtttgca acagatccag gttaccgata
    51601 atgccataca gcggcgttcg cagctcatga ctgacggtgg caaggaacat cgattttgac
    51661 tggctcgcct gttccgctgc ttgtgccatc tcctgcaacg actcttccat cttcacgcgc
    51721 gaagaaacat ccaccagcac acaaatggcc acgttttcat tacgatagcg cgaatggacg
    51781 aagctgattt gcagattggt attgttgctg gtcaggacat caacaaaatt gacctgctgc
    51841 ccacagataa tttgcgtcag tcgttggcgg tcctcatgcg taagcatatt gagataggta
    51901 tgcgccagtt cgttacttaa aatattgacg ccatcagcgg tacgcaaaat gcagataccc
    51961 actggcgcgg aggcgacaat cttgcgattg aactgctcat gttcttccag tcgcagggcg
    52021 tcgctttccg ccggaatgaa aatacgtcgc tcgtacatcc gcgcgagagt aaacaatgca
    52081 gctccggcaa gcacattcag caaaattgcg ttaaggatca acatgcgaat gcgttccagc
    52141 accttatcaa ccggcaccga atacacgatg cttagcgatg agggtggcag atttttcttc
    52201 agcaccagct cccggaaccc ttccgtatag ccaaaccagg agcgttcctg catccagcga
    52261 ggatcgccct taattttact ttctggtccg gtaagcgaaa tcagggtatg accattttca
    52321 tcaagaatgg taacccccat cggcaacgta cccggtaaga aaaagttctc catccggatg
    52381 gtctgctcga cacccaaaag cgcctgcaac cggttcgcca gataaactgg cgtcaacgcg
    52441 taaaaatacc cgacgcaagg gcgcggacct tcgctgatcc agtagaggtt actgccgcta
    52501 tcatcttgtg gtgcatttcg atatttattg atgcgttcat gcaacgcttt caacgcggta
    52561 tcgcgttcca ctggcatatc acgcagaccg aaattggcca tgcagaggtt atcactgccg
    52621 attaaaaata cccggttgag atcgtaagcc gcagaaaaat tatcgcgcca gtagcgcata
    52681 aaccacgcca atgactccag agaacctcgc caggtgttac tcattgcgga acaatcggag
    52741 tcggcaaaca gcggttcaaa cgcaggcaca tccgcctgcg tttctcgtcc acgcggggaa
    52801 agcacaccgt tttctgccga taagcgattt tcggcgatgt acttaagctc tttcatcaca
    52861 tcagaggtgc gttgaataaa gcgctgagcc tgatcggaac tcagattaaa ttcctgacga
    52921 atttccgatt ctcgctgatg taacgcatta acgatgtaaa aaacggatga aaaagcaatc
    52981 aacagccaga gcactaacgc caatgctctg aacatgtagc gcgaggcttt cagggttgta
    53041 cgaaaagaag caaggtattt caaaggggag aagctccgcc tcaggtgacc gatggagtgt
    53101 ggttaaggta gcggtaaaag cgtgttaccg caatgttctg tcaccattgc aataatgcgt
    53161 atcgcttaga aaccattgcc ttaaccagga aagccggagt agatggacag ttctcagtac
    53221 gaaaaataag aaatgaccaa tctgccgacg cttgttaatg gttatatcct ggcgtcggca
    53281 gatcgcccag cagtcttatc tgaaaaaaga gagagccagg ttgtgtgaag ggatggatca
    53341 ccagtcaaaa cttttcacca gcgtcagctc gccagcatta cgcatcggta caataaatgt
    53401 ttcctgtttc tcattgaccg atccttcatc ggtgatcagc gtcagttggg cggtcgtcaa
    53461 ttccgtttcg ctgcgcccac catagtagtt gatatacacc tgatagcgcc cgtgaactgg
    53521 tgctggcatg gcgaaaatct cgggtccgta ccccgtcgtg acatccatat ccagtgcacc
    53581 actgtttttc agcacggtgt taccgtacca ggcgtgttcg ccatcgggcg tgacaacatg
    53641 gagatcgaga tcggtattgt ccgtatccca cgagagaacc agccgtaaac gtgcacgaat
    53701 agttcctgta cccggcgttg agtaaaactg cattttttgc cggctttgcc catccgggct
    53761 gatgacctgc acgctgttgc tgccttcagt gaaaatataa gggcgtgcaa atgaaccatc
    53821 ggattcaatt ctctgcggca tactggcacc gttgaccacc agccggccct gctgaacttt
    53881 acccgccgca gcataattct ttattttgcc gcggatctgc gctgagatat tttgatcatc
    53941 tgccatgttg acggacgacg ccgggtaatt gatcgattgt gaaaaactgg catcttcgcc
    54001 ctctgccgga tgccagccag agagcggtgc gtcaatctcg acttcctgca caccttcact
    54061 gtgagcgaca ggcgaaagtg ccaccagtaa caacggaaga aaaatctttc gcatagcatt
    54121 agtccattaa taattgtcgg gtcagcgttt cgatatagtt ctcatcaagc ccggtgggat
    54181 aaccatcaaa ggccaagtgc agatactcat gggttaaatc cagccgatcc tgaagcgtaa
    54241 agaagttacg aataaacagc cgtttctgct gccgatcggt ataggggaaa cctgagacta
    54301 aacgacagac cgcaaacacg tctggttcgt tgtaccccgt ctcgccttgt aatatacgcc
    54361 gccactgcgg catttttttc gccagccagg ctttcgcttt gggtaataac tggcaggttg
    54421 agcgtggagc tccccagcgg ctaaggctgt tgtcgggata agcaaacgcg agtatctggt
    54481 cgtaacgctc cccctgcccc gcctgggccg tggcttgccg ccaggaaagt gtcccttcgg
    54541 tagcgcgact gccatgataa tgaaccggat cgcctgcata gatgagctcc tgcgtccagg
    54601 cggtcatcgt tcgcgccccc gtggtcgctg gcgaagcaga aacacgctgc gtggcgctgc
    54661 tgtcggggat ggtcaggcaa tcaccttccc gattggcgtt ttgctgtaaa aaagtacgta
    54721 tcgccacagt catggctttc gctgcctcag gtggcgtact tttcgcctcg cgatccagaa
    54781 ctctggcaac gtactcttca cgatcgagat gcgactgcaa tttcagtttg cccttttcgc
    54841 tcagtaaggt cgtctcaccg tggctgacaa acgtaatgtg gttaccatta gtaaacgtca
    54901 cacgatagcg cccattaagt acgctgggtt ttactgccgt cgtactcttt tccgctgtga
    54961 ttttcttcag cggatagcgg gcaaacagct ccacctctac acattgccca ctggcaacct
    55021 gcgtcggcac tggcaaaacc cgattaagca ccgtcgcata acgggttagt accgttttgc
    55081 tggtgccact gccagtaacc cacagcggtg taccatcggt taaccagcca gcaaaaccac
    55141 cctgacggat ttcctgttta tcatcggcaa accagctcca ggttttcacc cgtacccgac
    55201 taccgagcat ggatgcgaca ccgattttcg cctcgtccag caccacatcc agtaagactt
    55261 cctgagcttt gttttgcgca ggtaacgtcg ctaatgaatc aagcagcgat ttaaccgtta
    55321 ccgaggtttc tggcttcagc gttgtaagag acgctaacca cgcaggtgcc tgacgctgtt
    55381 gccagtattg cccccacaca tctgcgccaa tatgcaaccg ctgcggcgca aaatagagcc
    55441 cacaggaacg taccagcgcg gtatcgcgcg ttatgctctc cccagcctga caacaataaa
    55501 cctcttccgg ggagtttccg cgacactggt agggctgctc cggttgatgg gtatcctcca
    55561 gccaggcgta aacatacagt ttccacaaac tccctaacgg cgtggtgagc gagtcaggta
    55621 atgctgagac tttggtcacg cctgatgacg acagctgata cagttgatcg tgctgcgcac
    55681 cgcgcagcac cagttgtaac ggcgtctcct ccgcaagcgt tggcagagta accaacgcca
    55741 atagccagac gattctgcgc cagttcactt atttcacctg catcccggtc cattcactcc
    55801 ctgccgcgac actttgctgc gcaggtgcat aggaacgcac ataacgagca ggaggcagaa
    55861 cgaattgccc tttttgcgag aagcgcagca aatggcggaa agtgaccgtt cccgtcagtt
    55921 ctttcaccgg caccatatag gccagttcgc ccatttcatt acgcgctttt tccagcaatt
    55981 gcccctgctg tttcgcggcg ttgggtttat tgactgaaat gccccatgtt gtgcgctcaa
    56041 cgtcagctcc cggcgggagc ggtacttcca cctgaccata gcgcagaact gcatcctgct
    56101 cgctggtaag cgtaatttca tcgagataca gcgcatcgct gtcaatctca ttgctggtca
    56161 ccggctgcag agtaaagctc atctcttctt caccggggat aagccgatac aactggcgtt
    56221 caacggtcac cggaatgtta ctttgttgag ccgtttttgc cggttcacgc cagcggacct
    56281 gcacatttgc ggcgataatt cgtcaccaaa agagagaatg tccggcacac cctaaccaac
    56341 ccaacgccag tcttcgcccc ctccagttaa cttatgttta gcccatgcgc ccgcaggcgc
    56401 aggcaaaaca actggaggca ttgtcgccat atatttcgcc agccagttca tggccagcgc
    56461 gcgctcaata gtggattgct cagcggtcaa accattaaaa tagcggccgt ctgggtagcg
    56521 tcaccaccgc tattgagcag cacaaccgtg tgcgccagcg gctgattgct gtttgccgct
    56581 ttatcccacg catattgctg aacctgtggc atcaccgtgg acttcaactg cgcttttttc
    56641 agcaacgtca tggttaagac gtttgccacc gcatctgcca gtggagattc cggtgtatcg
    56701 aggatcaggc tgtcattgat atcgcggatg tcttcctcag agaaatcttc atctttagtt
    56761 ccgcgccggg cgatagcttc atccccccct ttcaacaacg ttttgcacgg cagattcatc
    56821 tcctgtgccc acgccagcac cagcgcccga tgcaataacg gcatattatc cgcctgctcg
    56881 gcataactgt cgagcatatg ctgccagtat tccggttgtt gcgttacgcc gagcgcctgg
    56941 ctggcctgcc agtcggcgta ccatgcccat gccgtaagga aggcgtcacc attgccatct
    57001 tcaccccacc aggtaaagcg cgctccgggc cccgccagtt gcatcagccg cagacggtta
    57061 tcctgaatca tctgacgaat gtcgttagcg gcggcacttt gatgatcggc aagcgaacgc
    57121 caggcgagac tgagcgggat cagacggcta ccggtgttga tcaccccacc ccacggttca
    57181 tcgactaacg catcaagatt gttgcggaaa atctcctgcg gcgtttcact actttgtagc
    57241 cggatattac tcgcctgctc gggcaacatc agcgcgttct cgccaccgcc gagcatgaca
    57301 tttttctgct gttcaacggg ccagctgttg tccacaaaag acagttttgt gctaatgctg
    57361 tcctgcactt gcccattttg ttgcagttct gcacttaaca agccagattg ctgaatattc
    57421 tgcgtcagcg aaatataatt cgcccctttg tgcagcgtca gcgtctggcg catctcagcg
    57481 cctgcaaatt tagtcaccag cgccaccggt tcgttatcct gctgactgaa gataaacagt
    57541 cctgccgccg gtttgtcgcc cacgcgatac accgttggca tactccactt catgtagaga
    57601 tttttttccg aacgcagata agcacgcccc tgcccgacca gcccgtcgcc gttcatccca
    57661 cgcgcggtga tacgccagcg ggttaacgaa tcaggcatca ggaacgtgaa atacgctttg
    57721 ccttgtttat cggttgtgag tgacggcatc catgccgcgg tatccacctc ttcacgccgt
    57781 ggacgttcaa gcatttttac tcgccgctcg ctgcggttag tcgcgccagg cgcaaccggc
    57841 tcgctggaga gcgcctggtc atagctgata aacgacagac tggagctggt acgcacattg
    57901 ttacgcccca gcggatagaa aaatttgccg atattcggcg cgatttctgg ttgcagcgcg
    57961 tagatcattt catcgaccac gcctaccgtt agctgcgcag aaacaggttt acctttcagc
    58021 gacgaggtta at
//
LOCUS       DAJTRD010000016        55364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTRD010000016 DAJTRD010000000
VERSION     DAJTRD010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.016"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..354
                     /locus_tag="OO315_001062"
     CDS             <1..354
                     /locus_tag="OO315_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5890065.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO315_001063"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO315_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5890066.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO315_001064"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO315_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5890067.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3109..3261
                     /gene="mokC"
                     /locus_tag="OO315_001065"
     CDS             3109..3261
                     /gene="mokC"
                     /locus_tag="OO315_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5890068.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO315_001066"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO315_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5890069.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO315_001067"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO315_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5890070.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="OO315_001068"
     CDS             6877..7281
                     /locus_tag="OO315_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5890071.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="OO315_001069"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="OO315_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5890072.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="OO315_001070"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="OO315_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5890073.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO315_001071"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO315_001071"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5890074.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO315_001072"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO315_001072"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5890075.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12134
                     /locus_tag="OO315_001073"
                     /pseudo
     CDS             10704..12134
                     /locus_tag="OO315_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12204..12980
                     /gene="yaaA"
                     /locus_tag="OO315_001074"
     CDS             12204..12980
                     /gene="yaaA"
                     /locus_tag="OO315_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5890076.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13133..13429)
                     /locus_tag="OO315_001075"
     CDS             complement(13133..13429)
                     /locus_tag="OO315_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5890077.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO315_001076"
     CDS             complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO315_001076"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5890078.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPTDFSALRKLMMNHQ"
     gene            complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO315_001077"
     CDS             complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO315_001077"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5890079.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO315_001078"
     CDS             complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO315_001078"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5890080.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKNISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18352..18471)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO315_001079"
     CDS             complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO315_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5890081.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18686..19372)
                     /locus_tag="OO315_001080"
     CDS             complement(18686..19372)
                     /locus_tag="OO315_001080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5890082.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19772..19912)
                     /locus_tag="OO315_001081"
     CDS             complement(19772..19912)
                     /locus_tag="OO315_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890083.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20008..20724
                     /gene="arcA"
                     /locus_tag="OO315_001082"
     CDS             20008..20724
                     /gene="arcA"
                     /locus_tag="OO315_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5890084.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO315_001083"
     CDS             complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO315_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5890085.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO315_001084"
     CDS             complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO315_001084"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5890086.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO315_001085"
     CDS             complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO315_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5890087.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO315_001086"
     CDS             complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO315_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5890088.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25003..25872
                     /gene="robA"
                     /locus_tag="OO315_001087"
     CDS             25003..25872
                     /gene="robA"
                     /locus_tag="OO315_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5890089.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO315_001088"
     CDS             complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO315_001088"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5890090.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26568..27088
                     /gene="yjjX"
                     /locus_tag="OO315_001089"
                     /pseudo
     CDS             26568..27088
                     /gene="yjjX"
                     /locus_tag="OO315_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO315_001090"
     CDS             complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO315_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5890091.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO315_001091"
     CDS             complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO315_001091"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5890092.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29737..31404
                     /gene="ettA"
                     /locus_tag="OO315_001092"
     CDS             29737..31404
                     /gene="ettA"
                     /locus_tag="OO315_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5890093.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31511..31678)
                     /locus_tag="OO315_001093"
     CDS             complement(31511..31678)
                     /locus_tag="OO315_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890094.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO315_001094"
     CDS             complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO315_001094"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5890095.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO315_001095"
     CDS             complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO315_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5890096.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO315_001096"
     CDS             complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO315_001096"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5890097.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35470..36114
                     /locus_tag="OO315_001097"
     CDS             35470..36114
                     /locus_tag="OO315_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5890098.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36142..37157
                     /gene="lplA"
                     /locus_tag="OO315_001098"
                     /pseudo
     CDS             36142..37157
                     /gene="lplA"
                     /locus_tag="OO315_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37189..37452
                     /locus_tag="OO315_001099"
     CDS             37189..37452
                     /locus_tag="OO315_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5890099.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO315_001100"
     CDS             complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO315_001100"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5890100.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO315_001101"
     CDS             complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO315_001101"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5890101.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO315_001102"
     CDS             complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO315_001102"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5890102.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO315_001103"
     CDS             complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO315_001103"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5890103.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42150..43691
                     /locus_tag="OO315_001104"
     CDS             42150..43691
                     /locus_tag="OO315_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5890104.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43663..44526
                     /locus_tag="OO315_001105"
     CDS             43663..44526
                     /locus_tag="OO315_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5890105.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44740..45522)
                     /locus_tag="OO315_001106"
     CDS             complement(44740..45522)
                     /locus_tag="OO315_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5890106.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45519..46592)
                     /locus_tag="OO315_001107"
     CDS             complement(45519..46592)
                     /locus_tag="OO315_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5890107.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46714..46875)
                     /locus_tag="OO315_001108"
     CDS             complement(46714..46875)
                     /locus_tag="OO315_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5890108.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO315_001109"
     CDS             complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO315_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5890109.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO315_001110"
     CDS             complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO315_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5890110.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO315_001111"
     CDS             complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO315_001111"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5890111.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO315_001112"
     CDS             complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO315_001112"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5890112.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO315_001113"
     CDS             complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO315_001113"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5890113.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51293..52324
                     /gene="rsmC"
                     /locus_tag="OO315_001114"
     CDS             51293..52324
                     /gene="rsmC"
                     /locus_tag="OO315_001114"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5890114.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            52601..52687
                     /locus_tag="OO315_001115"
     tRNA            52601..52687
                     /locus_tag="OO315_001115"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52635..52637,aa:Leu,seq:cag)
     gene            52716..52802
                     /locus_tag="OO315_001116"
     tRNA            52716..52802
                     /locus_tag="OO315_001116"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52750..52752,aa:Leu,seq:cag)
     gene            52837..52923
                     /locus_tag="OO315_001117"
     tRNA            52837..52923
                     /locus_tag="OO315_001117"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:52871..52873,aa:Leu,seq:cag)
     gene            complement(52962..53198)
                     /locus_tag="OO315_001118"
     CDS             complement(52962..53198)
                     /locus_tag="OO315_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5890115.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            53339..54127
                     /gene="fhuF"
                     /locus_tag="OO315_001119"
     CDS             53339..54127
                     /gene="fhuF"
                     /locus_tag="OO315_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5890116.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            complement(54165..54842)
                     /gene="bglJ"
                     /locus_tag="OO315_001120"
     CDS             complement(54165..54842)
                     /gene="bglJ"
                     /locus_tag="OO315_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5890117.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(54800..>55364)
                     /locus_tag="OO315_001121"
     CDS             complement(54800..>55364)
                     /locus_tag="OO315_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5890118.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
BASE COUNT        13247 a        14940 c        13816 g        13361 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaagtc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagcaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga tttaataccg cgagtaccgc aattgctaca gccgcgacgc caagggaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cactaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aagccagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcat catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcca ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcgcc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ctctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggtgctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcgttcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagtggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagca tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggcgcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcaccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc gcgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacaat ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc agtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccaccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgtgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tatcgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 tgggaatggg atcgacgcca aacgcggcag cggcagctgc atcctggcct ccgcatccgg
    11581 cagcacaagg gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg
    11641 caagcgccat gctgatatta ctggcgggta acggcacaac ctacatgccg ctggaaggta
    11701 ttcagcttat ccagaaggcg atgcgggtgc taatgggttc ctagggtgct gaatttgtta
    11761 ccctcgtggt tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa
    11821 acaatatctt ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca
    11881 ccttcgcaac ggtcatcggc ggcaccttgc taagccttcc gctgatgtgg caactggcag
    11941 atatcataat ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg
    12001 tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt
    12061 ttgatccgtt gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg
    12121 tttcgcagga gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca
    12181 taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt
    12241 accaaagccc gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc
    12301 agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca
    12361 gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc
    12421 cggcgaatgc ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg
    12481 ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg
    12541 gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga
    12601 tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca
    12661 acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag
    12721 atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt
    12781 tcctcgatga gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg
    12841 gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt
    12901 ttaatagcga aggttacttc tttgatgaag attcctccag caatggcgaa ctggtgttta
    12961 aacgctacga gcagcgttaa gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc
    13021 ggcctacaaa agcatgcaga ttcaatatat tgcagaatta acgtaggcct gataagacgc
    13081 gccagcgtcg catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt
    13141 gatgtttgcc tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg
    13201 gcggtggcgg cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc
    13261 accagccgtg gtcgcgccag tggcctccat cccagtaata gccacgatta tcacgatcgc
    13321 ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag
    13381 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
    13441 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt
    13501 attctccgca atcctaatct ctaagttacc gcatttattg ataatttctc ctttttccct
    13561 gtcgtttttc tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc
    13621 tgattgagat aatgaataga ttttactgat gattcatcat caatttacgc aacgcagaaa
    13681 aatcggtggg cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg
    13741 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
    13801 cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg
    13861 ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt
    13921 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
    13981 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
    14041 acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat
    14101 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgaatgac ttcgccagca
    14161 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
    14221 cctcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc
    14281 tgatgttaat cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa
    14341 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga
    14401 tattgccgcc caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
    14461 agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact gcagctccgg
    14521 tatcaccgga ggtcgcggtc agaatggtca ctggcttatc atccgcaata tgggtcagca
    14581 tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt
    14641 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc
    14701 gcacgcgctc ttccaggatt tcctgtggga tttcatcacc aataaacgcc gagaggatct
    14761 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt
    14821 ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt
    14881 gcgcaaagct gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca
    14941 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
    15001 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag
    15061 cgaacaaggt cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg
    15121 cctgccgcgc ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat
    15181 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag
    15241 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
    15301 gggcttctgc cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa
    15361 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa
    15421 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc
    15481 ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcaatgttca ttcatcgcca
    15541 tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct
    15601 tttccagagt catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc
    15661 actgataaac gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgagtt
    15721 tgttgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
    15781 caacgggtgc caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag
    15841 ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
    15901 tcggcaaaga caccggcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac
    15961 ggcagcggct gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg
    16021 aacagcggat cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca
    16081 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca
    16141 aagagatcgt cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta
    16201 aactctgcgg gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
    16261 tcacgagcga gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg
    16321 tccggttcgg tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct
    16381 tcgtctaact tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc
    16441 aattcatcac ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
    16501 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga
    16561 tagtaatcca tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg
    16621 cgcaggaagt cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc
    16681 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
    16741 ttggcttgtg caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc
    16801 agagccttcg agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag
    16861 ctttgctgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
    16921 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
    16981 gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
    17041 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc
    17101 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc
    17161 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct
    17221 cgcacacagt cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
    17281 atcagcacca cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgcca
    17341 accatccctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa
    17401 atgcccttga ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct
    17461 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg
    17521 gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac
    17581 atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca
    17641 acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag
    17701 tagtcggaac cgttacgtcc aagcaccacc agttcgcctt tttcattacc ggcggtgaaa
    17761 cctgccatca acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac
    17821 tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga
    17881 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
    17941 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
    18001 ctaatgccat gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat
    18061 tgcgccagcg ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata
    18121 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatgttttt ttcaatcatc
    18181 gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc
    18241 ctggcattgc tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat
    18301 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
    18361 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
    18421 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
    18481 tgtactctgt aatttttatc tgtctgtgcg ctatgcctat attggttaaa gtatttagtg
    18541 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
    18601 agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
    18661 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
    18721 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt
    18781 cgaccagttt tatgtcatct gccactgcca gagtcgtcag caatgccatg actcgttcgc
    18841 gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgccggt tgttgtatta
    18901 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
    18961 ccgccaccat cggcacacca gtaagcacgt cagccaacgc taactcttcg ttagtcagcc
    19021 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
    19081 acagcggcac cagttctact ggcgtggcgt agtaatgata tttcgcccga ctacgcgcag
    19141 tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
    19201 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg
    19261 cctgactatc gacaatccgc agttcgctaa aacccatcgt tttcattgcc cgcgccgctg
    19321 ccccaatatt ttctgctctg gcgggtgcga ccaggataat cgttatacgc atattgccac
    19381 tcttcttgac caaataaccg cgaacagggt gatcactgtc aacttattac gcggtgcgaa
    19441 tttacaaatt cttaacgtaa gccgcagaaa aagcccttta cttagcttaa aaaaggctaa
    19501 actattttct gactgcacta acggtttaat tgttaaaaaa tgctacatat ccttctgttt
    19561 acttaggata attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt
    19621 gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga
    19681 tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag
    19741 ttttattggg tgtccggccc ctcttagctt gttatgttgc tgttaaaatg gttaggatga
    19801 cagccgtttt tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt
    19861 tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc
    19921 gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg
    19981 atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac
    20041 gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc
    20101 gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg
    20161 atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag
    20221 caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc
    20281 ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg
    20341 attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt
    20401 cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg
    20461 atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac
    20521 ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc
    20581 cgtgagctga aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat
    20641 ttcgaatcta cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc
    20701 ttctgcggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta
    20761 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt
    20821 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtctatatta cgagtcacaa
    20881 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
    20941 cagagttgag cagtccccac atcactccat ccagcaacaa cagcgcgagg gtaaacaaca
    21001 tgctattgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
    21061 tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga
    21121 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
    21181 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg
    21241 cgtacttagt cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc
    21301 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
    21361 tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt
    21421 tggctggtag aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
    21481 cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca
    21541 tattcagctg caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct
    21601 gcccgccttg ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag
    21661 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc
    21721 ctaagatgat atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta
    21781 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
    21841 tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc
    21901 gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa
    21961 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
    22021 tacggtaatc ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca
    22081 gcaatactgc accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct
    22141 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
    22201 agtgacggtg aagtcgtagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
    22261 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
    22321 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
    22381 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg
    22441 cgcttagcgt tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc agtaaattcc
    22501 ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg ggcataacat
    22561 gcaaagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata
    22621 atgccgccac atcaacagca gtcagaacga cttcctgacg attctccagt cttgcctggc
    22681 gtagtaacgt ttccaccagc gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa
    22741 aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
    22801 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
    22861 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
    22921 taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
    22981 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc
    23041 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    23101 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg cccataatcg
    23161 gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg
    23221 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa
    23281 cggctttatt tgccgaatcg aacaatactt tgccatgcgc atcggtcata tagacatgat
    23341 attcattgcg cactttgtta atgccaccaa tattggcgcg aaacgggcga tgttgtagct
    23401 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    23461 ccagctccgc aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc
    23521 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    23581 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aagcccctca ggctatatcc
    23641 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    23701 cagcgtttta atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    23761 gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    23821 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    23881 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt
    23941 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    24001 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    24061 cgtcaggaac agtacaggta acgccggatg gagcgccagt aactggcggc acaattcaaa
    24121 gccactgata tcaggcaaac caacgtcgag aatcatgacg tcgggaacct gctggcgagc
    24181 tttatccagc accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    24241 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    24301 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    24361 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    24421 ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    24481 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    24541 ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat
    24601 accaccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    24661 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    24721 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    24781 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    24841 aataacatat tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca
    24901 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    24961 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    25021 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    25081 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    25141 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    25201 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    25261 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    25321 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca
    25381 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    25441 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    25501 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    25561 agtcaggata aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag
    25621 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    25681 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg
    25741 tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    25801 gcagaagacg ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct
    25861 atccgccgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    25921 gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    25981 atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta
    26041 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    26101 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    26161 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    26221 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    26281 cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg
    26341 gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc
    26401 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    26461 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    26521 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    26581 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    26641 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac
    26701 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    26761 tacttccaga ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt
    26821 tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc
    26881 cgttacctgc ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc
    26941 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    27001 cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    27061 tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat
    27121 tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc
    27181 ttttcagcaa cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt
    27241 tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac
    27301 tcatttcgcc acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct
    27361 catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct
    27421 taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat
    27481 agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc
    27541 ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg
    27601 tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc
    27661 atcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac
    27721 aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg
    27781 tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata
    27841 ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct
    27901 gctataacca ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg
    27961 catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg
    28021 ggcaatcgcc atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag
    28081 atcgttgtaa gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt
    28141 tgcctgaacg ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg
    28201 ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag
    28261 attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc
    28321 aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc
    28381 aaccatcggg tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc
    28441 ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt
    28501 tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag
    28561 cgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc
    28621 gcgccatttc gcctgctcat cggtgacatc attgcccatc aaacgccagg cgacgatatc
    28681 gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat
    28741 cagccgtgca ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg
    28801 acgggtaaaa tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt
    28861 cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag
    28921 cactgttacc aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc
    28981 cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg
    29041 ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc
    29101 ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg
    29161 gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga
    29221 ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac
    29281 cgccggttga ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg
    29341 ataatccttc agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc
    29401 ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct
    29461 gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt
    29521 ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga
    29581 tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg
    29641 cgaaggtgat cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa
    29701 ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg
    29761 catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt
    29821 ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg
    29881 ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac
    29941 atcaagattg gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag
    30001 tccattgaag aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat
    30061 gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg
    30121 gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg
    30181 gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt
    30241 cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa
    30301 ccgaccaacc acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc
    30361 gaaggcaccg tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg
    30421 atcctcgaac tggaccgcgg tgaaggaatt ccgtgggaag gtaactactc ctcctggctg
    30481 gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg
    30541 atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa
    30601 gcacgtctgg cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc
    30661 aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac
    30721 ctgcgtaaat cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa
    30781 ggggcgatcg tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg
    30841 atctccggtc aggaacagcc ggacagcggc accatcactt tgggtgaaac ggtgaaactg
    30901 gcatcggttg atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt
    30961 tccggcgggc tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt
    31021 ggccgcttta actttaaagg ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt
    31081 gagcgcggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc
    31141 gacgaaccaa ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg
    31201 gagttcccgg gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc
    31261 acgcacatcc tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact
    31321 gagtacgaag agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc
    31381 aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga acgccttatc
    31441 cggcctacaa aaccagcaat ttcaatacgt tgtaaaactg taggcctgat aagacgcggt
    31501 aagcgtcgca tcaggcattg agcaccgaat gccggatgcg gcgtgaacac cttttccggt
    31561 ctacaacagc cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac
    31621 cttgtttaat ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt
    31681 tatacctcgc ctttgagcag tttcgtcacg gttatccttg ctcccccatc atctcccgca
    31741 ccagctccac gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt
    31801 cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg
    31861 aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca
    31921 ccagatcgaa acggtattca tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt
    31981 actttttgca gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc actttattgg
    32041 cgtatttcac tgcaaaatca atgtattgtg cgtgacccaa cgcgatttta tcgtagtcgg
    32101 aatactgcaa tgcgatctca tcaccgccga ggtgtgaaaa gacataatcg cggccatatt
    32161 cccacgcact ggtggtgttg aagatattgg caagtttgtt taccagggtg gatttaccgc
    32221 tcgactcgcc gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcggtaggaa
    32281 tatattccca gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg
    32341 tacgtttcgg atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat
    32401 cggcttcttc cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt
    32461 tgatgccgtt gctccacaca tcccagccat gcggatacgg ctccatgccc tcttcgttga
    32521 aagcatgaat acggatattt ttctgatatt taaaggtttg caatagccag cgcagacgat
    32581 ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat
    32641 cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt
    32701 agatatgtcc ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct
    32761 gccggggaaa ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc
    32821 ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag
    32881 ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg
    32941 acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag
    33001 ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg
    33061 aacaatcgcc cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct ggccactggg
    33121 caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag
    33181 cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc
    33241 ggttaccttc acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc
    33301 accgtgacgg tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc
    33361 cacgcggcgc ggattcgcca ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa
    33421 cggacgcgtt ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg
    33481 acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa
    33541 gacgcccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg
    33601 ggagtcggca tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttcgg
    33661 gccagccagc gaaccatctt tggttacgtg tccaaccatg acaatcgcca caccgcgcgt
    33721 tttggcgaaa cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga
    33781 tgattgtaca tccgccatat gcatcacctg gatcgagtca attaccatca gcttcggttg
    33841 ctcttcttcg gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt
    33901 gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc
    33961 ggtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt
    34021 ggatttcccc gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc
    34081 tcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct
    34141 gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg
    34201 ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc
    34261 atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac
    34321 aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg
    34381 atgacgggca ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat
    34441 caggtcagcg tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta
    34501 ggcaatcccc agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc
    34561 cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt
    34621 gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc
    34681 cagttcattg gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa
    34741 agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag
    34801 ctgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc
    34861 agcgcctttc agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg
    34921 catcgcccgt tcggttacct ccgccaccat ctcgcccgtt ccggccagct tggcaatttc
    34981 atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt
    35041 gcgcaggtgc gggattttcc ccagcggggc gacatccagc tgcgcttcgt gggccaggcg
    35101 tgtagcccgt gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca
    35161 ggcggcaaca atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt
    35221 atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat
    35281 cacttcatca ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag
    35341 gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat
    35401 aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa
    35461 gggtggaaga tggctcgcac aaaactgaaa ttccggctac atcgggcagt gattgtcctg
    35521 ttctgtcttg ccttgttagt ggcactgatg cagggggcgt catggtttag tcaaaaccac
    35581 cagcgacagc gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg
    35641 ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc
    35701 ctcgatcagt taacagatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc
    35761 gatcttatcg cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt
    35821 aaaaaagcag gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga
    35881 ccgctcggct atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag
    35941 gtggataaca ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta
    36001 gtgctgaccc gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg
    36061 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
    36121 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac
    36181 ccgtggttta acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc
    36241 gttctgtttc tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg
    36301 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc
    36361 ggtggcggcg cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag
    36421 ccggagtacg ataaaactat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc
    36481 gtcagcgccg aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc
    36541 aaagtctcag gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg
    36601 ctactcaatg ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg
    36661 gcggcgaaag gcattacttc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg
    36721 gggctcacca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg
    36781 agcacgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg
    36841 aaacctttgc ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc
    36901 atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag
    36961 gccatatcac ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg ctggaagccc
    37021 tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag
    37081 cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag
    37141 cgggggcggt aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg
    37201 gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc
    37261 gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg
    37321 attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa
    37381 atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct
    37441 gaacataaat gaatagattc gtgaatagat acttgaatgt tttttatttt ctatccatat
    37501 gaaaaataat agtaaatcta taaatcagtt gctattgatg aatagatact aaaaagccgg
    37561 agcagtcccc cactccggct taacacgaca atcgcctttc agcgaaacac aattactctt
    37621 tatcgcccag cagaacggat tccagtgcga ttttgatcat gtcgttgaag gtggtctgac
    37681 gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca
    37741 gggctttcgc gccaaactct gcagccacgc cgtagatacc agccgcttcc atttccacgc
    37801 cgaggatgcc gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt
    37861 cagcggagaa caggttaccc acgcgagcat caacgcccag tgctttagct gcatctactg
    37921 cgttacgcac catgtcaaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc
    37981 ggttaacttt ggaatcggtg caggccccca taccgataac gacgtcgcgc agttttacgt
    38041 gcggcagaac tgcgccacag gaaccaacgc ggataatttt cttcacgccg aaatcggtga
    38101 tcagttcttt ggtgtagatg gagcaggacg ggatacccat accgtgaccc attacggaaa
    38161 ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac
    38221 gggcatcttc aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca
    38281 aaactacgtc agcgaaatcg cccatttctg cattaatgtg tggggtagcc attgttttat
    38341 cctttttgtg acataacaaa ggcggagtca acgctccgcc caaatccatc agaacatggc
    38401 tttgccatat tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc
    38461 gaaggtttca cggtgaccca gtgagcccgg ttttactttc gggccatata ccagtaccgg
    38521 aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt
    38581 gaggatcagg atgtcgtcat cgcgcagcag agacatcagc tccggcagac ggcggtcgaa
    38641 cagttccaga cccgcagcat aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa
    38701 gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt
    38761 ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt ttggtgatac cgcagttggc
    38821 gtagatgtct gcaattttac cgacagaaac cacctggccg tgtttttcat caaccagttt
    38881 ctgcagcacg gtcggtgctg gcggctcaac agccaagtcg tgacggttac cggtacgctg
    38941 gaagttacca gctttgtcac cgataaacgg acgagcgata acacgaccga tattgtagcc
    39001 gccgttggtc agctcttcac gggcgatttc gcacagttcg tagagtttat ccagaccgaa
    39061 agtttcttca tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt
    39121 gccggttttc atgtgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca
    39181 gttaccgagg taacccggca gattagcgcg ttcgaccagt ttatccagca gctcttgcgg
    39241 gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac agaaccggga caccggcgat
    39301 ttcccagtga ccagacgggg tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc
    39361 gccgataact tcagcgttgc cgtccattcc cgccggaatg aaaccggtag aaccttcgtg
    39421 tgctttcgcc agccccagac gggtcagatt tggcagattg agcggccctt tacgaccatt
    39481 atcagcttcg cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc
    39541 accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa
    39601 tgcacgtttc atatgttctc cgtactcagt gcttcaaatt taaatgatca gatcagtata
    39661 ccgttattcg ctgatacggc gatagacagt tggtgtgctt tccggtgcgt tatcggcaag
    39721 tttaattgcc gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag
    39781 gataaccgcc agcggacgct gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa
    39841 gccgacgctg taatcgatgg tgtcagatgc ctgacgacga ccgccgccca ttgcaactac
    39901 tgccatcccc agcgcgcggg tatccatttc actgacaaaa ccttcggtat cagcatagac
    39961 tgctttcgtc agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg
    40021 gcctttttgt gccgctacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac
    40081 cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg agatcaacat
    40141 ctccacgcac agcgccatcg tgacatcaaa cagacgcggg ttacgatact cacccgtcag
    40201 gaactgcacc gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt
    40261 catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc
    40321 ttcggcaagg gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac
    40381 gtccatcacc agcgcgtcca gaccttccgc cagtttcttc gccaggatcg aggcagtgat
    40441 cagcgggatg gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc
    40501 cggagccagt gaactggtct gaccgataat cgccacgccg acgtctttaa taatttcgcg
    40561 gaaacggttg tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt
    40621 accgccagta tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cgcaggctgc
    40681 gaccatcggc cccaacatca gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc
    40741 aacaatcggg ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat
    40801 cgccatggtc agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc
    40861 gagggcggca atctgccctt cggaaatagt gttgtcgcga ataccgttga taaagaaacg
    40921 aatttcttca tcgctcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag
    40981 aaacaaggta acctcccgag gaaaagagta tgaggcggct tttgccggat gcgtctacgc
    41041 cttatccggc ctacgggtag atacgtctcg taggcctgat aagcgcagcg catcaggcgt
    41101 aaagcatctt acttagtagc tgctggcgct cttaccgtcg ccgtggccca gcgctttcag
    41161 caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc
    41221 accgaacagt tcatctgcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc
    41281 gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcacggatca cttccatcat
    41341 gatgcgagcg ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa
    41401 gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag
    41461 ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca
    41521 ggctttcacc aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa
    41581 caccacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat
    41641 ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt
    41701 gccctgctct ttcagagttt tgcgagcaat cgggataaaa cgaggataga tacagatagc
    41761 ggcggtattg ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt
    41821 gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt
    41881 cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt
    41941 agtattctaa catctactcc gcaacacact tcgatacaca tcacaattaa ggaaatctac
    42001 ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc
    42061 gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg
    42121 catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac
    42181 aacgttgcca gcaaattgtc accagcccag tgcttagccc ggagcagaag cgccattttc
    42241 tggcactgga agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg
    42301 cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct
    42361 atgtcttacc cgattacgct cgttttctgg cgaacggttc cgaatggctg gagctggaag
    42421 gcgcgaaaga tcttgatgac gcactctctc tgctgaccat tctttaccac cacgtaccgt
    42481 cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta
    42541 gaattctaac acaaaacgag atcgatgttc gaataaaacg tttctggcgt tacctcgaca
    42601 gaaccctgcc agatgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc
    42661 gcgcgatctt acgtgcagac gcgcaggttt caccgaacct gacctttatc tacgatcctg
    42721 aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac
    42781 cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg
    42841 tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc
    42901 tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc
    42961 actactgcca gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat
    43021 cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg
    43081 tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gagaaagaag
    43141 ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg
    43201 cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca
    43261 tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc
    43321 cgtatggcga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg catcatgctt
    43381 attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaacccgc
    43441 aggcactggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca
    43501 atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag
    43561 aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac
    43621 gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct
    43681 ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg
    43741 tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt
    43801 tgcaatgact gtggggagtg cgtgccacag tgtccgcatc aggcgttgca gattgttgac
    43861 ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt
    43921 ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg
    43981 cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagc cacgacccaa
    44041 ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccatctc
    44101 acctgtctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attactcccg
    44161 gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca tcaacaatta
    44221 accggacgcg ataatcaaca gattaagcgc agcatctatt tgctggcaga gcgcggcaag
    44281 ctggcggaac tgcgtttgct gatgatccca ggccaggtgg attatttgca acacatcgaa
    44341 gaactggcgg cgtttatcaa gggacttggt gatgttccgg ttcgcctgaa cgcgtttcat
    44401 gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag
    44461 ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat
    44521 ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcctg ataagacgcg
    44581 tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg
    44641 gcctacggtt cggcacaaac ctgtaggcat gataagacgc gttaagcgtc gcatcaggca
    44701 ttatgcgccg actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac
    44761 cgcatacgtg ttattaagca acacttcggc aatctcatcc gcgggttccg ggcgtaactc
    44821 gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc
    44881 gttgagcggc atatccggcg catcggtttc cagcaacaac gatgccagcg gtaattttgc
    44941 gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt
    45001 gtagcccagc tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc cgtggacaac
    45061 accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg
    45121 tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa
    45181 ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccga tctcccccac
    45241 cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgctcaa gagacacatc
    45301 gctatgtttt tccaacatac caggatgcaa gcccaatacg gcatacagcg gttgataatt
    45361 ttccgctaac gcctgcacac gggcaaaatt ttccgcctct gtcgccggaa caatgatctt
    45421 gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg
    45481 gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc aagccagatc ctcattgtta
    45541 aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg
    45601 actggcgcag gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta
    45661 agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa
    45721 cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt
    45781 aatggcttcg gcggataaat ttcggatatc cgcagcttgc ccggtggttt ctcaataaat
    45841 tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg
    45901 ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt
    45961 gacggcacag tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc
    46021 ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta
    46081 cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt
    46141 ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg cgcacatata aaacgatttg
    46201 ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc
    46261 tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa
    46321 aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag
    46381 ataaacgccg agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta
    46441 aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg
    46501 ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata
    46561 ttaccaagcg ttacaggtat tcgctgcccc acttctgcct tcctgttttt gttgtgaaag
    46621 aaagacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg
    46681 gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa
    46741 actcactagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt
    46801 accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat
    46861 gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg
    46921 tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc
    46981 gggaggacga attacttagt tttcagatca tttttaacgc ttttcacacc atctaccgct
    47041 ttggcgatac tttcagcacg gtcactttgt gcctgagaat ctacggtacc ggagagctga
    47101 accacgccat cggtggtttc aactttcact ttacgtgaag ggacgatatc gtccgccagc
    47161 agtttggctt tgatttcact ggtggtggcg gtgtcacccg cgtagccctt caccgagcct
    47221 tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg tcacgccttc aacgcctttc
    47281 gccactttca ctgcctcttc ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg
    47341 actttttgat cggttttgac agagatatcg gtgctcttga tgttgtcatg atccaccagc
    47401 gccgccttca ctttcgcggt gatggcgctg tcatccatga aattaccgac tttattcata
    47461 gagctatcga ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag
    47521 gcagagccgg tcgcgacggc agaggtcaac attacagcca gcagagtttt cgaaatcttc
    47581 agtcttgtca tagtcatcga tttattcctg tatgtttgct cgtaatttga gcctagcaac
    47641 acgaggttgc attgctgaat agggagagac ttcaccctct acagaagtca atggtcgcca
    47701 tcacaaaagc gatgggtgat gaataacgac cattacagcc tctgaatcag ttattaatat
    47761 cggtagaatg acaatcgacg gctttagata ctaatatcta cgcattgaac ggtatttaac
    47821 gccatcagaa atgtcatcac tttgttaaat atagatcaca attttgaaac cgctcgggat
    47881 atcagcgaaa acataagcaa aagtgaatgt tttaagaaca ttccgtaagc ggctaataag
    47941 gaagggaaat tgacagggcg cagcggctac cgcgccctgg aggcaagaat taatgctcgc
    48001 gggtctggtg gaactgaacg tccggataac gttcctgtgc caggcgcagg ttgaccatgc
    48061 tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc cagttggctt tcgttcttac
    48121 gcttgaactc ttcgaatttc ttcgcgtctg cacattctac ccagcgggca gtggcaacgt
    48181 tgactgactc atacactgct tcaacgttgt attcgctctt caggcgcgct accaccacat
    48241 caaactgcag cacaccaacc gcaccaacga tcagatcgtt gttggagatt ggacggaaca
    48301 cctgcaccgc gccctcttcg gaaagctgta ccagcccttt gagcagctgt ttttgcttca
    48361 gcggatcttt caggcggata cgacggaaca gttctggtgc gaagttcgga ataccggtga
    48421 acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca
    48481 ggccgaggat atcgcccgga tacgcttctt caacgtgcga acggtcaccc gccataaagg
    48541 tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt
    48601 tttcatattt accggacacc acacgcataa acgccacgcg gtcgcggtgt ttcggatcca
    48661 tgttggcctg aattttaaat acgaagccgg taaatttatc ttcgctcgct tctacggtac
    48721 gggtatcggt ctgacgcggc atcggcgcag gcgcccactc caccaggcca ttcagcatat
    48781 gatcaacgcc gaagttaccc agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa
    48841 ggaacagctc tttgtcgaac tcgttagacg cgcctttaac cagttccagt tcgtcacgca
    48901 gctgctgtgc cagatcttca ccaaccgcag catcgagatc cgggttattc agccctttaa
    48961 caatgcggac ttcctgaatg gtgtgccctt taccgctctg atagagatag gtttcatctt
    49021 tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc aattggccaa gtgatcggtg
    49081 cgcagccaat tttcagctcg ttctcaactt catcgagcag ttccatcggg tcgcggatat
    49141 cacggtcaag tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca
    49201 tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc gataaccatc aggcagcagt
    49261 ccaccgccgt cagggtacga taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga
    49321 gcaggttaac caggcaatcg tgatacggaa actgcatcac agacgtagta atggagatcc
    49381 cacgctgctt ttccatctcc atccagtccg acttagcgtg ctggttggaa ccacggcctt
    49441 ttactgtacc ggcggtctga atggcctgtc cgaacagcag caccttctcg gtgatggtag
    49501 ttttaccggc gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc acctctttca
    49561 aataaggaga caacgtcata attctttctt cttgagtaag cggcatcgcg ccgcgcttgt
    49621 tgaaaacgaa aaattgcggc tattttaccc atcaacgggg gggaggcaat cagtgtttac
    49681 acaggagctg ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg
    49741 gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag cccggcgttg atgccaccga
    49801 gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca ggattgcccg
    49861 cctgttccag cgcataatcg aaaattttct tattcggttt ggcaacgcca acttcttcgg
    49921 aaatcaccag caaatcgaag taatcacgca ggcccgtccg ttccagacgc acttgctgca
    49981 aggcgctaaa accgttggtg atgatgccga ttttagcgtt gctacgaatg gcgttaagca
    50041 gagaaaccgc gcccggcaac ggcgtacaga tttccgccat cgcattaata aaggcatcgt
    50101 taagcaagcc tgcttcaact ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa
    50161 gctgtaatga agtgatcgcg ccgttttgat aatccaccca cagtggcttg ttaacggcct
    50221 gatagtcctg aaaatcttca gcggtaaagg tgacgctgta atcaagaaac atccgctgca
    50281 ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa atccagtccc
    50341 acttcattat tccaccttgt cttacatact gattggcaac gccatgatga tggcgtcttc
    50401 gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact
    50461 ttcgtacagg gcaatggcgg cagcatttga ggcacggact tccagccata gtgtcgccac
    50521 gccgcgtttt tccagttcgt cgatcagatg ttccagcagc gccctaccca gcccctgacg
    50581 ctgatagtcg ggatcgaccg caatattgaa caatgtagct tcatccagca ccacttgcgt
    50641 aatcgcaaac gccgccattt tgccgttttg cgttaactga aagttgagat aacgctcgcc
    50701 ctggttgctg gcaaaggttt tttcactcca cggaaaggcg tgggcgcgtt gttcaatgtg
    50761 gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt
    50821 gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg
    50881 ccacctgagc gccttccaat gatagcggtt cgtcagtacc caaccgccag ctgttgcagc
    50941 gactgccttg cggcagcatc gcgatttttt ctggcgtcag ttgcagcact tgatcggggc
    51001 tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg ggaagatcgt
    51061 ttgccaccat caccagacgg acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg
    51121 cgccagggcg acgcagcgac cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc
    51181 gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata
    51241 ccgcgccaac atatggctat aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc
    51301 atttaccccg gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac aaagccgtat
    51361 tctgtttgcc ggagacttac aggatgacct gcccgcgcgt ttagataccg cggccagccg
    51421 tgctcatacc cagcaattcc accattggca ggtgttaagc tgccagatgg gggataacgc
    51481 ccgttttagt ctggtcgcca cggcgaatga tgtcgcagat tgcgatacgc tgatttacta
    51541 ctggccgaag aacaaaccgg aagcccagtt ccagttgatg aatttacttt ctctgctgcc
    51601 agtggggaca gatatttttg tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca
    51661 gatgctggca gattatgcgc cgttgaataa agtcgacagc gctcgtcgct gtggcctcta
    51721 ttttggtcgt ctggaaaaac agccagtatt tgatgcggat aaattctggg gcgaatacag
    51781 cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt
    51841 cggtagccag ttgctgctct cgacgttaac tccgcacacg aaaggtaaag tgctggatgt
    51901 cggctgtggc gcgggtgtac tttcagttgc ctttgcgcgc cattcgccga aaattcgtct
    51961 caccttgtgc gatgtctctg ccccggcggt agaagccagc cgcgcaacac ttgcagccaa
    52021 cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga
    52081 tatgatcatc tccaacccgc cgttccacga tgggatgcaa accagcctgg atgcggcgca
    52141 aacgctgatt cgtggcgcgg tgcgtcatct taatagcggc ggcgagctgc gaattgtagc
    52201 gaacgccttc ctgccttacc cggacgtgct ggatgagaca tttggcttcc atgaagttat
    52261 cgcgcaaacc gggcgcttca aggtatatcg cgccattatg acccgccagg cgaagaaagg
    52321 ttgagtatct gatatctgtc gccgcatacc gtgtcggatg cggcgtgaac gccttatccg
    52381 acctacaaaa cctgcacgtt agcccttcgt aggccagata agacgcgcca gcgtcgcatc
    52441 tggcatctgc ccccgcatgc ctaccaaacc tgcacgctag tttcctgatg gacatttttc
    52501 cagcaattac acatcaggcg ataattaact attgacgaaa agctgaaaac cactagaatg
    52561 cgcctccgtg gtagcaattc tttttaagaa ttgatggtat gcgaaggtgg cggaattggt
    52621 agacgcgcta gcttcaggtg ttagtgtcct tacggacgtg ggggttcaag tcccccccct
    52681 cgcaccaaaa atcacgttga tattgctcgc actgggcgaa ggtggcggaa ttggtagacg
    52741 cgctagcttc aggtgttagt gttcttacgg acgtgggggt tcaagtcccc cccctcgcac
    52801 caaacgaggc gatatcaaaa aaagtaagat gactgtgcga aggtggcgga attggtagac
    52861 gcgctagctt caggtgttag tgtccttacg gacgtggggg ttcaagtccc ccccctcgca
    52921 ccaattatct ttacttcctt tcttgtttct tccctgtttt gtcatccctg attcaaattc
    52981 attatcatca gcatgatgcc agcaataagc gccaggcacg atggtagcaa aatgtaatgc
    53041 cgcaactgtt tgtgatacag catcaccggc gtcaatacta atcccatcag aatgacaatc
    53101 cgccactgat ccggcgataa atccgcatac atcagccctg caatcagcaa tcccggcagc
    53161 aacactcccc agccactgcc cagcgtacgt tgcaacatgg tttcatctct ttcattgata
    53221 atgaaaacca atatcatatg ataattttta tcatttgcaa gccagagaaa tcccttgcta
    53281 tcgagtaaac ctatcgctag gattagcaat cattatcatt tagattacta tcacgattat
    53341 ggcctatcgt tccgcaccgc tctatgaaga tgtcatctgg cgaacgcatc tccagccgca
    53401 ggatgctggg cttgcacaag ccgtacgggc gatgatcgcc aaacatcgtg aacatttgct
    53461 ggagtttatc cgcctggatg aacccgctcc gcttaacgcc atgacgctgg cgcaatggtc
    53521 atcaccgaat gcgctaagtt ctctactggc ggtctattcc gatcatatct atcgcaacca
    53581 accaacgatg atccgcgaga acaaaccgct gatctcacta tgggcacaat ggtatatcgg
    53641 cctgatggtg ccaccattaa tgctggcgct actgacgcag gaaaaagcat tagatgtgtc
    53701 gccggaacat ttccatgctg agtttcacga aaccggacgc gtcgcctgtt tctgggtcga
    53761 tgtgtgtgaa gataaaaacg caacaccaca ttcgccgcag cagcgaatgg aaacgttaat
    53821 cagccaggcg ctggttccgg ttgtgcaagc actagaagcg accggagaga tcaacggcaa
    53881 acttatctgg agtaataccg gttatttgat caactggtat ctcactgaga tgaagcaact
    53941 gctcggcgag gctactgttg aatcgctgcg ccatgccctc ttttttgaga aaacgctcac
    54001 gaacggtgaa gataatccac tctggcgtac cgtggtactg cgcgacggcc tgctggtgcg
    54061 ccgcacttgc tgccagcgtt atcgcttacc ggatgtgcag caatgtggct attgtacgct
    54121 gaaatgaaag cactgccggg gaagtaaacc cggcatcatg cggattaata gggatgcaac
    54181 acattacttg cttcgcaatg ccgcatacat aacagaatat cttcggcctc caacaaccct
    54241 gcatcagaac tgacgcccag tttcgacatc acattaaatt tatgcgctcg gatcgttttg
    54301 atattacgtt taagctgctc ggcaatttgt gtcattgagt agccacgcga cataaagcgc
    54361 aatatttctc tctccgtcgg gcttaacgtc cggctttggt taatgtacca ctgattgttc
    54421 agtcggtcgg tcgcctgacg tacaccattt aatgacaaaa agagttcctg atgaaaaatc
    54481 tccagcgttg acgctttact taatacaccg tccagcggtg atggcgacaa tgaaccaatc
    54541 agccgagctt caatatcatc atccacaata actaaacgcc gggtacgcgg aaacttaatc
    54601 gccagctcag tcaggcaaga taatccctct ctgcgctcac ttctcatggc agaaaaagaa
    54661 aaaatgaccg ccgcaaacga gatccgcgac atggcatcct gaaatgactc ctgactggta
    54721 taggtatgga gcttataagg gttgcccgca aactttctga ataaactctc aataccgata
    54781 ctactcatga cgcatttttc tatgagtgcg acatttctct tcttaattcg gctgtgttcc
    54841 attctgcggc atctccataa acagaaggca agttaagttc tcttgcaccc tgcgcgctat
    54901 ttatccatgc atacatttca gcgttgctgt gaattgccag tcgacgcatc gcactgtttt
    54961 tttgggcgct gatagtttta ttacttttct taagcaatga agctatctgg ttgatgcccc
    55021 atcctttacc taatagccgc agtaccttgc gctctgaaag agtgagaatg acggagcgat
    55081 agctaaaatc atgagtttca gtcattgccg gagaggtcag catggcgctg atacgctctg
    55141 cgtgcgtatt gccggaacgt acggtcttga ccagattctc aatgggttca acatcagaca
    55201 ataacgtcgc cgtagggcac atgagcagtt ctacagcctg ggaataccag gagcgtgaca
    55261 ccatgaaaac ccagtgaatt tcccgatatt gtgagattaa agaataataa tgttcacaaa
    55321 cagaacgtgg atcttcactt tcaccggcta aatcagcaat gact
//
LOCUS       DAJTRD010000017        55167 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTRD010000017 DAJTRD010000000
VERSION     DAJTRD010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55167
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.017"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..1234)
                     /gene="argH"
                     /locus_tag="OO315_001122"
     CDS             complement(<1..1234)
                     /gene="argH"
                     /locus_tag="OO315_001122"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5890119.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQ"
     gene            complement(1295..2071)
                     /gene="argB"
                     /locus_tag="OO315_001123"
     CDS             complement(1295..2071)
                     /gene="argB"
                     /locus_tag="OO315_001123"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5890120.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2079..3083)
                     /gene="argC"
                     /locus_tag="OO315_001124"
     CDS             complement(2079..3083)
                     /gene="argC"
                     /locus_tag="OO315_001124"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5890121.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3237..4388
                     /gene="argE"
                     /locus_tag="OO315_001125"
     CDS             3237..4388
                     /gene="argE"
                     /locus_tag="OO315_001125"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5890122.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4740..7391
                     /gene="ppc"
                     /locus_tag="OO315_001126"
     CDS             4740..7391
                     /gene="ppc"
                     /locus_tag="OO315_001126"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5890123.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7574..9307
                     /locus_tag="OO315_001127"
     CDS             7574..9307
                     /locus_tag="OO315_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5890124.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9456..10307
                     /locus_tag="OO315_001128"
     CDS             9456..10307
                     /locus_tag="OO315_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5890125.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10294..10635)
                     /locus_tag="OO315_001129"
     CDS             complement(10294..10635)
                     /locus_tag="OO315_001129"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5890126.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10637..11515)
                     /locus_tag="OO315_001130"
     CDS             complement(10637..11515)
                     /locus_tag="OO315_001130"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5890127.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11481..13778)
                     /locus_tag="OO315_001131"
     CDS             complement(11481..13778)
                     /locus_tag="OO315_001131"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5890128.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(13829..14149)
                     /locus_tag="OO315_001132"
     CDS             complement(13829..14149)
                     /locus_tag="OO315_001132"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5890129.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14164..15243)
                     /locus_tag="OO315_001133"
     CDS             complement(14164..15243)
                     /locus_tag="OO315_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5890130.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15552..16655
                     /locus_tag="OO315_001134"
     CDS             15552..16655
                     /locus_tag="OO315_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5890131.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16656..18053
                     /gene="ptsP"
                     /locus_tag="OO315_001135"
     CDS             16656..18053
                     /gene="ptsP"
                     /locus_tag="OO315_001135"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5890132.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18065..18727
                     /gene="fsa"
                     /locus_tag="OO315_001136"
                     /pseudo
     CDS             18065..18727
                     /gene="fsa"
                     /locus_tag="OO315_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18738..19839
                     /gene="gldA"
                     /locus_tag="OO315_001137"
                     /pseudo
     CDS             18738..19839
                     /gene="gldA"
                     /locus_tag="OO315_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20114..20721
                     /locus_tag="OO315_001138"
                     /pseudo
     CDS             20114..20721
                     /locus_tag="OO315_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(20748..21653)
                     /gene="yijE"
                     /locus_tag="OO315_001139"
     CDS             complement(20748..21653)
                     /gene="yijE"
                     /locus_tag="OO315_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5890133.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21747..23927)
                     /gene="katG"
                     /locus_tag="OO315_001140"
     CDS             complement(21747..23927)
                     /gene="katG"
                     /locus_tag="OO315_001140"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5890134.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24256..25146)
                     /gene="metF"
                     /locus_tag="OO315_001141"
     CDS             complement(24256..25146)
                     /gene="metF"
                     /locus_tag="OO315_001141"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5890135.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25495..27927)
                     /gene="metL"
                     /locus_tag="OO315_001142"
     CDS             complement(25495..27927)
                     /gene="metL"
                     /locus_tag="OO315_001142"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5890136.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(27930..29090)
                     /gene="metB"
                     /locus_tag="OO315_001143"
     CDS             complement(27930..29090)
                     /gene="metB"
                     /locus_tag="OO315_001143"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5890137.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29367..29684
                     /gene="metJ"
                     /locus_tag="OO315_001144"
     CDS             29367..29684
                     /gene="metJ"
                     /locus_tag="OO315_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5890138.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            29868..30476
                     /locus_tag="OO315_001145"
     CDS             29868..30476
                     /locus_tag="OO315_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5890139.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30537..30749)
                     /gene="rpmE"
                     /locus_tag="OO315_001146"
     CDS             complement(30537..30749)
                     /gene="rpmE"
                     /locus_tag="OO315_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5890140.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            30952..33150
                     /gene="priA"
                     /locus_tag="OO315_001147"
     CDS             30952..33150
                     /gene="priA"
                     /locus_tag="OO315_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5890141.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33306..34331
                     /gene="cytR"
                     /locus_tag="OO315_001148"
     CDS             33306..34331
                     /gene="cytR"
                     /locus_tag="OO315_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5890142.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34423..35382
                     /gene="ftsN"
                     /locus_tag="OO315_001149"
     CDS             34423..35382
                     /gene="ftsN"
                     /locus_tag="OO315_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5890143.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35475..36005
                     /gene="hslV"
                     /locus_tag="OO315_001150"
     CDS             35475..36005
                     /gene="hslV"
                     /locus_tag="OO315_001150"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5890144.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36015..37346
                     /gene="hslU"
                     /locus_tag="OO315_001151"
     CDS             36015..37346
                     /gene="hslU"
                     /locus_tag="OO315_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5890145.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37413..38339
                     /gene="menA"
                     /locus_tag="OO315_001152"
     CDS             37413..38339
                     /gene="menA"
                     /locus_tag="OO315_001152"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5890146.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38432..38917
                     /gene="rraA"
                     /locus_tag="OO315_001153"
     CDS             38432..38917
                     /gene="rraA"
                     /locus_tag="OO315_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5890147.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39002..39247)
                     /gene="zapB"
                     /locus_tag="OO315_001154"
     CDS             complement(39002..39247)
                     /gene="zapB"
                     /locus_tag="OO315_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5890148.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39673..40518
                     /gene="glpF"
                     /locus_tag="OO315_001155"
                     /pseudo
     CDS             39673..40518
                     /gene="glpF"
                     /locus_tag="OO315_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40558..42048
                     /gene="glpK"
                     /locus_tag="OO315_001156"
     CDS             40558..42048
                     /gene="glpK"
                     /locus_tag="OO315_001156"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5890149.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42278..43288
                     /gene="glpX"
                     /locus_tag="OO315_001157"
     CDS             42278..43288
                     /gene="glpX"
                     /locus_tag="OO315_001157"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5890150.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43385..44131
                     /gene="fpr"
                     /locus_tag="OO315_001158"
     CDS             43385..44131
                     /gene="fpr"
                     /locus_tag="OO315_001158"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5890151.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44136..44564)
                     /gene="uspD"
                     /locus_tag="OO315_001159"
     CDS             complement(44136..44564)
                     /gene="uspD"
                     /locus_tag="OO315_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5890152.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44591..44890)
                     /locus_tag="OO315_001160"
     CDS             complement(44591..44890)
                     /locus_tag="OO315_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5890153.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45102..45542)
                     /locus_tag="OO315_001161"
                     /pseudo
     CDS             complement(45102..45542)
                     /locus_tag="OO315_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45643..46242
                     /locus_tag="OO315_001162"
     CDS             45643..46242
                     /locus_tag="OO315_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5890154.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKIQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46350..47117
                     /gene="tpiA"
                     /locus_tag="OO315_001163"
     CDS             46350..47117
                     /gene="tpiA"
                     /locus_tag="OO315_001163"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5890155.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47172..47927)
                     /gene="cdh"
                     /locus_tag="OO315_001164"
     CDS             complement(47172..47927)
                     /gene="cdh"
                     /locus_tag="OO315_001164"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5890156.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48034..49023)
                     /gene="sbp"
                     /locus_tag="OO315_001165"
     CDS             complement(48034..49023)
                     /gene="sbp"
                     /locus_tag="OO315_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5890157.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49343..50305)
                     /gene="pfkA"
                     /locus_tag="OO315_001166"
     CDS             complement(49343..50305)
                     /gene="pfkA"
                     /locus_tag="OO315_001166"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5890158.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50486..51388)
                     /gene="fieF"
                     /locus_tag="OO315_001167"
     CDS             complement(50486..51388)
                     /gene="fieF"
                     /locus_tag="OO315_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5890159.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51536..52036)
                     /gene="cpxP"
                     /locus_tag="OO315_001168"
     CDS             complement(51536..52036)
                     /gene="cpxP"
                     /locus_tag="OO315_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5890160.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52186..52884
                     /gene="cpxR"
                     /locus_tag="OO315_001169"
     CDS             52186..52884
                     /gene="cpxR"
                     /locus_tag="OO315_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5890161.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            52881..54254
                     /gene="cpxA"
                     /locus_tag="OO315_001170"
     CDS             52881..54254
                     /gene="cpxA"
                     /locus_tag="OO315_001170"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5890162.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54360..55034)
                     /gene="yiiM"
                     /locus_tag="OO315_001171"
     CDS             complement(54360..55034)
                     /gene="yiiM"
                     /locus_tag="OO315_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5890163.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13427 a        14946 c        14109 g        12685 t
ORIGIN      
        1 tctgcaactc actgagtggc agatcttcca gcggtttgcc ctgacgaatg gcttccacca
       61 ccgcttcacc cacaatatgg tgcgcctcgc ggaaaggtac gcctttcgcc accaggtaat
      121 ccgccagttc agtggcgttc gcatacccct gctgcgccgc ttcctgacaa cgcggacgtt
      181 tcacctgaat gccgtccagt accagcgccg ccatatgcag gcagtccagc caggtatcga
      241 gcgcgtcgaa cagaccttct ttgtcttcct gcatatcttt gttgtaagcc agcggcaaac
      301 ctttcagcgt catcatcatg ccggttaacg caccctgcac ccggccgcat ttaccgcgaa
      361 tcagctccag cgcatccggg tttttcttct gcggcattaa tgatgaaccg gaagtcacgc
      421 ggtccgaaag ctccacaaac cccgcttcgc cggtgttaaa gaaaatcaga tcttcagcaa
      481 aacgcgacag atgcaccatg ccgatagcgg cagcagaaag cagttccaac acatggtcac
      541 ggtcagaaac gctgtccaga ctgttacggg tcgccgaagc aaagcccagc cagcctgcta
      601 actgttcacg gtcgatttca taggccgttc ccgccagcgc gccacagcct aatgggctga
      661 catccagacg cttaagcgca tcctgcaaac ggctttcatc acgcgccagc atctcaacat
      721 aggccaggca ccagtgcgcg aacgtcaccg gctgggcgcg ttgcaggtga gtgtaacctg
      781 gcattaccgc gtcctgattg ttttgtgcgg tttccaccag cgccgattgc agctgccggt
      841 tagccgtcag taactcgcta acggtatctt tgcaccacag tttcaggtca gtcgctacct
      901 gatcattacg gctacgcccg gtatgcagct ttttgcctaa ctggcccact ttgtcgatca
      961 gtttgccttc cacccagcta tggatatctt cggcgtcgct ttcaaggatt tgttgtggcc
     1021 tggcgcgaac atcttccagc aacacgttca gcgcctcttc cagttgcgcc tgctcttctg
     1081 cggttaacac gccgaccgtt accaaggctt tggaccaggc cacagagcca acaatatcct
     1141 gctccgccag acggtaatca aagcgcagtg agtcgttgaa ttgtttgaac cgttgatctg
     1201 ctgcctgggt aaaacgcccg ccccaaagtg ccataactct gtttccttat tttgaaattc
     1261 aatgccggaa agctgcgcct ccggctaaca aaacttaagc taaaatccgc gtacccatcg
     1321 gcataccgtt aaacagtgcc ggaagctgct ccgcataacg ccaagaagcg atatctaccg
     1381 gacggcccag cgtgcgggcc gcatccagcg ccgcgttcac tttcactatc atgccgtcag
     1441 taataatgcc ctgctcaatc agttgttctg ctttcgcggc ggtcatttcg gcaatgcgtt
     1501 gccctttgcc gtcgagaatg ccgctgacgt cggagagcaa aatcagatcc gcgcccagcg
     1561 ttgccgccag cgccgttgcc gcctggtcgg cattgacgtt catcagttgc ccttcgtctg
     1621 ttacgccaat ggagctgacc accggcagat aaccgttctc cagcaaggag ttgataagct
     1681 taggcgaacc tggctgcgcc agtccaacat gacctaactc ttcatcaagc tgggtcactt
     1741 tgacgctgtc gccgtcaccg agaaacaaac ctacggccgc aatctgatgt ttcttcgccc
     1801 atgccaacag ggttttattt gccgttcccg ccagtgctcc ggtgataatg tctatctgat
     1861 cagcaggcgt cacccgcagg ccgtttttct ttttcaccgg cagattcagc cctttcatca
     1921 gctcatccac cacgcaaccg ccgccgtgca caatcaccag cggacgctga tgtgactcac
     1981 gataattcac caatgcgcta aacagacgtt ccagcgcctc ttcactatcc agcagtacgc
     2041 cgcccagttt gataattaat ggattcatca ttgcaccctt aaataagaga ctgcgtttcc
     2101 gcatagccga aacgaatatt ggcgcactgt accgcttgtg ccgccgcgcc tttcagtaag
     2161 ttgtcttcgg tcgccacaat aatcagatgc tcgccctgaa cggcaaaccc gatatcgcaa
     2221 aatggcagcc caacgacatt tttcagcgcc ggaacgcctt tgtcatacag ccgcaccagc
     2281 ggtttatggg catacgcctg ctgtaacgct tgcgcgactt gcgcctgggt cacacccgat
     2341 ttcaggcggc aggtaatggt ttcgagaatg ccgcgcggga aattgcccag atgtggggtg
     2401 aagataacgt cagcaccgag gtgtgtggcg atctctggtt gatggcgatg agtaaagacg
     2461 ccatacggtt gcaggctaac ttcacaaaaa ctatttgaaa tcgctgcctt acgtcctgca
     2521 ccgctcacgc cgctggtggc gttgatcacc ggccactgat tgaggtcaag aagatcggca
     2581 tcaatcaacg gtttcagcgc cagctgtgcc gccgtcggat aacagcctgg caccgcaatc
     2641 aaattcgctt cttttaattt attaccgcac cactccgcca gaccgtaggc tgcctgttcc
     2701 agcagttccg ggtattgatg ggtaaagccg taatattttt catagaaggt ggcgtcgtta
     2761 acacgaaacg cgccggaaag gtcgaacacc acgcagcccg cttcaagaaa ctgcggcgct
     2821 aaatcgtggc taacttcatg ggcggtggcg agaaacacta cgtccacccc tgggctaaac
     2881 tcgctgatat ccgacatcgg ctgcaacggc agctcaacga tgccttttag ctgcggatgc
     2941 aaatcggaga ttaactttcc cgcatcattg ctttgcgctg aaacagtcaa agcggttatg
     3001 ttcatatgcg gatggcgatt tacataggtc actagctctg cgccagcgta gccgctggca
     3061 cccacaatca gcgtattcaa catcggggct attcaccttc ttatgtctgg ttgccaggtt
     3121 aaacgtaaaa cattcacctt acggctggtg ggttttatta cgctcaacgt tagtgtattt
     3181 ttattcataa atactgcatg aatattgata ctatcatgac cagaggtgtg tcaacaatga
     3241 aaaacaaatt accgccattt atcgagattt accgcgctct gattgccaca ccttcaataa
     3301 gcgctacgga agaggcactc gatcaaagca atgcagattt aatcactctg ctggcggact
     3361 ggtttaaaga tttgggcttc aatgtggaag tgcagcctgt tccaggaact cgcaacaaat
     3421 ttaatatgct ggccagttac ggacaggggg ctggcggctt gttgctggcg gggcataccg
     3481 atacggtgcc atttgatgac ggtcgctgga cgcgcgatcc gtttacactg acggagcatg
     3541 acggcaagct ttacggctta ggcaccgccg acatgaaagg cttttttgcg tttatccttg
     3601 atgcgctacg cgatgtcgac gtcacgaaac tgaaaaaacc gctctacatt ctggcgactg
     3661 ctgatgaaga aaccagtatg gccggagcgc gttattttgc cgaaactacc gccctgcgcc
     3721 cggattgcgc catcattggc gaaccgacgt cactacaacc ggtacgcgca cataaaggtc
     3781 atatctctaa cgccatccgt attcagggcc agtcggggca ctccagcgat ccagcacgcg
     3841 gaattaacgc tatcgaacta atgcacgacg ccatcgggca tattttgcaa ttgcgcgata
     3901 acctgaaaga acgttatcac tacgaagcat ttaccgtgcc ataccctacg ctcaacctcg
     3961 ggcatattca cggtggcgac gcttctaacc gtatttgcgc ttgctgtgag ttgcatatgg
     4021 atattcgtcc gcagcctggc atgacactca atgaacttaa tggtttgctc aacgatgcat
     4081 tggctccggt gagcgaacgc tggccgggtc gtctgacggt cgacgagctg catccgccga
     4141 tccctggcta tgaatgccca ccgaatcatc aactggttga agtggttgag aaattgctcg
     4201 gagcaaaaac cgaagtggtg aattactgta ccgaagcgcc gtttattcaa acgttatgcc
     4261 cgacgctggt gctcggtccg ggttccatca atcaagctca tcagcctgat gaatatctgg
     4321 agacgcggtt tattaaaccc acccgtgagt tgattatcca ggtaattcac catttttgct
     4381 ggcattaaaa cgtaggccgg ataaggcgct cgcgccgcat ccggcaccgt tgccaaactc
     4441 cggtgccgca ataatgtcgg atgcgatgct tgcgcatctt atccgaccta cacctttggt
     4501 gttatgtatg gcgatttttt aacatttcca taagttacgc ttatttaaag cgtcgtgaat
     4561 ttaatggcgt aaattcctgc tatttattcg tttgctgaag cgatttcgca gcatttgacg
     4621 tcaccgcttt tacgtggctt tataaaagac gacgaaaagc aaagcccgag catattcgcg
     4681 ccaatgcgac gtgaaggata cagggctatc aaacgataag atggggtgtc tggggtaata
     4741 tgaacgaaca atattccgca ttgcgtagta atgtcagtat gctcggcaaa gtgctgggag
     4801 aaaccatcaa ggatgcgttg ggagaacaca ttcttgaacg cgtagaaact atccgtaagt
     4861 tgtcgaaatc ttcacgcgct ggcaatgatg ctaaccgcca ggagttgctc accaccttac
     4921 aaaatttgtc gaacgacgag ctgctgcccg ttgcgcgtgc gtttagtcag ttcctgaacc
     4981 tggccaacac cgccgagcaa taccacagca tttcgccgaa aggcgaagct gccagcaacc
     5041 cggaagtgat cgcccgcacc ctgcgtaaac tgaaaaacca gccggaactg agcgaagaca
     5101 ccatcaaaaa agcagtggaa tcgctgtcgc tggaactggt cctcacggct cacccaaccg
     5161 aaattacccg tcgtacactg atccacaaaa tggtggaagt gaacgcctgt ttaaaacagc
     5221 tcgataacaa agatatcgct gactacgagc gcaaccagct gatgcgtcgc ctgcgccagt
     5281 tgatcgccca gtcatggcat accgatgaaa tccgtaagct gcgtccaagc ccggtagatg
     5341 aagccaaatg gggctttgcc gtagtagaaa acagcctgtg gcaaggcgta ccgaattacc
     5401 tgcgcgaact gaacgaacaa ctggaagaga atctcggcta caaactgccc gtcgaatttg
     5461 ttccggtacg ttttacctcg tggatgggcg gtgaccgcga cggcaacccg aacgtcactg
     5521 ccgatatcac ccgccacgtc ctgctgctca gccgctggaa agccaccgat ctgttcctga
     5581 aagatattca ggttctggtt tctgaactgt cgatggttga agcgacccct gaactgctgg
     5641 cgctggttgg cgaagaaggt gccgcagaac cgtatcgcta tctgatgaaa aacctgcgtt
     5701 ctcgcctgat ggcgacacag gcatggctgg aagcgcgcct gaaaggcgaa gaactgccaa
     5761 aaccagaagg gctgctgaca caaaacgaag aactgtggga accgctctac gcttgctacc
     5821 agtcacttca ggcgtgtggc atgggtatta tcgccaacgg cgatctgctc gacaccctgc
     5881 gccgcgtgaa atgtttcggc gtaccgctgg tccgtattga tatccgtcag gagagcaccc
     5941 gccataccga agcgctgggc gagctgaccc gctacctcgg tatcggcgac tacgaaagct
     6001 ggtcagaggc cgacaaacag gcgttcctga tccgcgaact gaactccaaa cgtccgcttc
     6061 tgccgcgcaa ctggcaacca agcgccgaaa cgcgcgaagt gctcgatacc tgccaggtga
     6121 ttgccgaagc gccgcaaggc tccattgccg cctacgtgat ctcgatggcg aaaacgccgt
     6181 ccgacgtact ggctgtccac ctgctgctga aagaagcggg tatcgggttt gcgatgccgg
     6241 ttgctccgct gtttgaaacc ctcgatgacc tgaacaacgc caacgatgtc atgacccagc
     6301 tgctcaatat cgactggtat cgcggcctga ttcagggcaa acagatggtg atgattggct
     6361 attccgactc agcaaaagat gcgggcgtga tggcagcttc ctgggcgcaa tatcaggcac
     6421 aggatgcatt aatcaaaacc tgcgaaaaag cgggtattga gctgacgttg ttccacggtc
     6481 gcggcggttc cattggtcgc ggcggcgcac ctgcccatgc ggcgctgctg tcacaaccgc
     6541 caggaagcct gaaaggtggc ctgcgcgtga ctgaacaggg cgagatgatc cgctttaaat
     6601 atggtctgcc agaaatcacc gtcagcagcc tgtcgcttta caccggggcg attctggaag
     6661 ccaacctgct gccaccacct gagccgaaag agagctggcg tcgcattatg gatgaactgt
     6721 cagtcatctc ctgcgatctc taccgtggct acgtacgtga aaacaaagat tttgtgcctt
     6781 acttccgctc cgctacgccg gaacaagaat tgggcaaact gccgttgggt tcacgtccgg
     6841 cgaaacgtcg cccaaccggc ggcgtcgagt cgctgcgcgc cataccgtgg atttttgcct
     6901 ggacgcaaaa ccgcctgatg ctccccgcct ggctgggtgc aggtacggcg ctgcaaaaag
     6961 tggtcgaaga tggcaaacag agcgagctgg aagccatgtg ccgcgattgg ccattcttct
     7021 cgacgcgtct cggcatgctg gagatggtct tcgccaaagc agacctgtgg ttggcggaat
     7081 actatgatca acgtctggta gacaaagcac tgtggccgtt aggtaaagag ttacgcaacc
     7141 tgcaagaaga agacatcaaa gtggtgctgg cgattgccaa cgattcccat ctgatggccg
     7201 atctgccgtg gattgcagag tctattcagc tacggaatat ttacaccgac ccgctgaacg
     7261 tattgcaggc cgagttgctg caccgctccc gccaggcaga aaaagaaggc caggaaccgg
     7321 atcctcgcgt cgaacaagcg ttaatggtca ctattgccgg gattgcggca ggtatgcgta
     7381 ataccggcta atcttcctct tctgcaaacc ctcgtgcttt tgcgcgaggg ttttctgaaa
     7441 tacttctgtt ctaacaccct cgttttcaat atatttctgt ctgcatttta ttcaaattct
     7501 gaatatacct tcagatatcc ttaaggaatt gtcgttatat tcggcgatat ttttttcaag
     7561 acaggttctt actatgcatt ccacagaagt ccaggctaaa cccctttcta gctggaaagc
     7621 cctgggttgg gcactgctct acttttggtt tttctctact ctgctacagg ccattattta
     7681 catcagtggt tatagtggca ccaacggcat tcgcgactcg ctgttattca gctcgctgtg
     7741 gttgatcccg gtattcctct ttccgaagcg gattaaaatt attgccgcag tgatcggcgt
     7801 ggtgctatgg acggcctctc tggcggcgct gtgctactac gtcatctacg ggcaggagtt
     7861 ctcgcagagc gttctgtttg tgatgttcga aaccaacacc aacgaagcca gcgagtattt
     7921 aagccagtat ttcagcctga aaattgtgct tatcgcgctg gcctatacgg cggtggcagt
     7981 tctgctgtgg acacgcctgc gcccggtcta tattccaaag ccgtggcgtt atgttgtctc
     8041 ttttgccctg ctttatggct tgattctgca tccgatcgcc atgaatacgt ttatcaaaaa
     8101 caagccgttt gagaaaacgt tggataacct agcctcgcgt atggagcctg ccgcaccgtg
     8161 gcaattcctg accggctatt atcagtatcg tcagcaacta aactcgctaa caaagttact
     8221 gaatgaaaat aatgccttgc cgccactggc taatttcaaa gatgaatcgg gtaacgaacc
     8281 gcgcacttta gtgctggtga ttggcgagtc gacccagcgc ggacgcatga gtctgtacgg
     8341 ttatccgcgt gaaaccacgc cggagctgga tgcgctgcat aaaaccgatc cgaatctgac
     8401 cgtgtttaat aacgtagtta cgtctcgtcc gtacaccatt gaaatcctgc aacaggcgct
     8461 gacctttgcc aatgaaaaga acccggatct gtatctgacg cagccgtcgc tgatgaacat
     8521 gatgaaacag gcgggttata aaaccttctg gatcaccaac cagcagacga tgaccgcccg
     8581 caataccatg ctgacggtat tttcgcgcca gaccgacaag cagtactaca tgaaccagca
     8641 acgtacgcag agtgcgcgtg aatacgacac caacgtgctg aagccgttcc aggatgtgct
     8701 gaatgaccct gcgccgaaga aactgatcat cgttcatctg ctgggtacgc atatcaaata
     8761 caaataccgc tacccggaaa atcagggcaa gtttgatggc aataccgatc atgttccgcc
     8821 aggattaagc gcagaagagc tggaatcata taacgattat gacaacgcta acctgtataa
     8881 cgatcatgtg gttgccagcc tgattaaaga ctttaaagcg gcagacccga acggattcct
     8941 ggtttacttc tctgaccacg gtgaagaggt ttacgacacg ccgccgcaca aaactcaggg
     9001 gcgtaacgaa gacaacccga cgcgccacat gtacaccatt ccgttcctgc tgtggacgtc
     9061 ggaaaaatgg caagcgactc atccccgtga tttctcacag gatgtcgatc gtaaatacag
     9121 cctggcggaa ctgatccaca cctggtcaga tttggcgggc ttatcttacg acggttacga
     9181 tccaacccgt tcagtggtga atccgcagtt caaagaaact acccgctgga ttggtaaccc
     9241 gtacaagaaa aacgcgctga tcgattacga cactctgccg tatggcgatc aggtgggtaa
     9301 tcagtagatg acctttgtat cgcgtctggc gctatttgcc ggatgcgata ctgccgcaat
     9361 gtttacatcg aactcccctc aggccacact tttcaggtgg ccttttttaa taatgccagt
     9421 tactgttttc ccgtctacat acctaaggga ataacatgta tcacgacgtc agctatctgc
     9481 tctcccgcct gatcaacggc ccgctgtccc tgcgccagat ttactttgcc agcagtaacg
     9541 gcccggtgcc cgatcttgcg tatcaggtcg attttccacg gctggaaatt gtgctggaag
     9601 gtgagtttgt tgataccggc gctggagcaa cgttagttcc cggcgatgtg ctgtacgttg
     9661 ccgctggtgg ctggaatttc ccacaatgga aaacacccgc taccaccttt agcgtgctgt
     9721 ttggcaaaca gcaactcggc ttcagcgtcg tgcaatggga tggcaaacaa tatcaaaatc
     9781 tggcgaagca acacgtcgcc cgacgcggcc cacgcatagg ttcttttctg ctacaaacgc
     9841 tcaatgaaat gcaaatgcag ccgcaggagc agcaaacggc aaggcttatc gtagccagcc
     9901 tgcttagcca ctgccgcgat ttgctcggca gccaaatcca gaccgcctca cgcagccagg
     9961 cactatttga agctattcgc gattatatcg acgaacgcta tgcctccgcg cttacccgcg
    10021 aatctgtcgc acaggcattc tatatttcac caaactatct ctcgcacctg tttcaaaaaa
    10081 cgggggccat tggttttaac gaatacctga atcacacgcg actggagcac gctaagacgt
    10141 tattgaaagg ctatgacctg aaggtaaaag aagtggcgca cgcctgcggc tttgtcgaca
    10201 gcaactactt ctgccgtctg tttcgtaaaa acaccgaaca ctcgccgtcg gaatatcgcc
    10261 ggcagtacca cagccaactg acagaaaaac cgactactcc agaataagat gggtttgctg
    10321 cggcgcagaa agcactttgc gcaccgcgct cattacccgc tgcggctcac gcaggaatgc
    10381 gtagatgctg cattgcacat agcggcaatg ttcaaatcgc tcggcaccgg caagctcgat
    10441 atccgtaatc aacagagcaa catccgcccg acggatatcc tcgtccgcta aacgattctc
    10501 cgttcccagc gcgccctgag tttcaatgct gacgccccac ttctctaact ggcacaactt
    10561 ttccagccgt tccgccgcca tataagtatg cgccacgccg cttacgcagg cggttactgc
    10621 caccaggtat gccattttaa cctcccacgg taacctgaaa tccggcccgt tctgccattt
    10681 cgcgcatcgt tgtcacatcg gctgacgacg gcgcaggcac ctctttcatc gaccatgttt
    10741 tccccagcag gcggtatttc ggttcgccgt actgatgaaa cggtaacaga tggatctgcc
    10801 tgattttcag cgggatcagt acatccagcg cctgctgcat attctcccgg ctgagcgtga
    10861 aaccagggat caacggtaaa cgcgggatca cgttgacacc ctcactcacc agcaaacgca
    10921 gattctccag cacgcgtggc aggttcatct tcaccacatc ccgcgcctga gtcgcgtcca
    10981 taatttttaa atcgaacagc acttcatcgc acaatttcgc cagcggtaac agcttgcttg
    11041 ctggcgcgtc tccggcagtt tcaatggcgc atgagacacc ccacagccgc agtcgctgta
    11101 aaaaacgggt agcaaactcc gcctgcatta acacttcgcc gccagaaagc gtgacgccgc
    11161 cgccggacgt gcgaaagaaa atgtcatctt tcatcacttc ccgttccaga gcgtcaaggc
    11221 tgatatcgcg accaatccgt tcaaacgccc cggaggggca ttcatccgca tcacgcaaac
    11281 atttcgcaca gtgcagacat ttcgcctctc tgcgtaccgt ctggattttg ccggagatcg
    11341 actccggatt agcacaccac gggcaaagat gcggacagcc tttaaaaaag accaccgtac
    11401 gaatgccctc gccgtcattc agtgaataac gctgaatgtt gaaaatgcgc gccacatcat
    11461 cgcggcgcgt ttccacaacg ttacagctga tgcgctgtcc ggcggatgat gtcatcctgg
    11521 atctccttcg acagttcgac aaagaaggcg ctgtatccgg caacgcgcac caccagcccg
    11581 gcataatctt gtgggcgctg ttgcgcttcc cgcaacgtgt cggcgttcac cacgttaaac
    11641 tgaatatgct gcaacttaag ctgggtaaac acccgtaaga agtcggccag tttgcgtaat
    11701 cccgcttcac cttccagggt cgccggagtg aatttcacgt tcagcaacgt accgttagac
    11761 agcagcgtat tatcgagctt actgactgac ttcagtaccg ccgttggccc ttgtgcgtcc
    11821 tgacccagca taggtgacaa gccgccgtct gccagttgtt ccccggcaaa acgaccgtct
    11881 ggcgtcgcgc caaccaccga tcccaacgga acgtgagcag aaacggtata cgatcccggc
    11941 gtgaagtaac cgccgcgcgg gttctggtat ttttccactt ctttgcagta gtggcgcaac
    12001 agttcggcgc taatgttatc cacctcgtcg atatcgttac cgtatttctc aaagcggtta
    12061 attaagcgag cgcggacttt ttcgccttct ggcgttgcaa agttggcttt taataccgac
    12121 agcaattcgt caaaacttaa acgctgttgc tcaaaaacca tccctttcaa cgcatggaga
    12181 gaatcgctca ggttggcgat accaattccc tgtacgccgg agaagttata acgcgcgccg
    12241 ccatcggtaa tatcgcgacc tttttccaga caatcgctga taaaagacga gagcagcggt
    12301 acaggtgccc agtcgcgatg gccgatatca caaatattgc tgccctcaac catcagggtg
    12361 atgtagtggc tgatcttggc gcggatctgc tccagtaaac cttcataagt cagcgcagca
    12421 ttgccttcat tttcatgcag gcagatttcc atcactttca gcagattaaa catcgcgatg
    12481 tcatgcaagc cgtaggttct gccgggaata gataattcca cacagcccac tacggaatag
    12541 tcgcgcgcat cttccagcga aacgccacgg ttgaggaacg ctggcaccac cacttcatcg
    12601 ttaaagattt gcggaatacc ggtgccgagg cgaatggttt cggcggtttt catcaggaac
    12661 ggcgtgtcga tcaaggcgtt agtgcgcacg ccgaggttcg gttgcggtaa ttgcacgctt
    12721 tgataggcgt caaggcaaag gaacgaaagc acgttcaccg cgctacgtcc gttctcggtt
    12781 aacccgccga gcagtgcggt atagccggtc gggaaaccgg cgaaataacg cgcgctactg
    12841 gtggagcgca acagcacgat gtcgttgcat ttcacccata aagattcgag cagttctttc
    12901 aggaacgccg gatcttcgcc ctgggttaaa gatgcctgat agaacggcaa catatactgg
    12961 tcgaagcgcc ctaaagacag cgaactggcg ttggattcgt attgcagaat gatgttcatg
    13021 taccagaata actggcacgc ctgccagaac gtctgcggct tatgttgcgc gttatggcgg
    13081 gagatctccg caatagtcag cagctcttcg cgacgctggg catctgtgca gtttgccgcc
    13141 atcgtttccg ccagttcggc gtaacgcaaa atgtgtttct gcgaggcttc cagcagtaac
    13201 aacgcggcct gataaaagtg attctccggc tgttgctgac aatgttgctg catctgtgcc
    13261 accagctcac ccagaccgtg attcaacagg cgtgggtaat caataataat gtgcccctgg
    13321 cctttatccg tctggttgat gctgaaaatc tgcgtgttgg tcgcggcttt tacttcatct
    13381 gtcatctgcc cgttgatgaa atctttcatc gaacgttttt cccagtacgg gaacaactct
    13441 tcacggtaga tacgtttatc ttcttcgctg atagcaaagc ggtcctgcgg acgcgtcggg
    13501 aattgatcca gctctttcag cagccagtaa gggtccattt ccggcgacat aatcccggcg
    13561 cgcggtttta cggtgcggtt accggcaatc agttcttcat cacgaatcga aatttcaaca
    13621 tgttcaagga tatacgctgt tgctttcgcc cggcgcaata tcaccggttc gccttcggtt
    13681 tgccgatggc tggcggtata aagcagcgcc cgctccagcg agatttcacg ggtattggca
    13741 aacagtgcag ttttgaggcg agagatacga ttcgtcatga ggaactcctg tgggaaagcg
    13801 aacccgccct tgcgagcggg agaagaactt aagcggtttg cgccaggtga gcttcaattt
    13861 tgctcataat ggcgtcagca cgttttactg catcgctgat gttgacgcgc acgatggtct
    13921 tgccagcaaa acgctcttca aacttgatgc cgatatcttt ggtcagaatc accatatcag
    13981 cgctcgccac gtcttccgca gtcagttcat tttccagacc aattgacccc tgggtttcta
    14041 ctttcacttc ccagcctttc gctttcgcgg cactttccag cgcctctgct gccatatagg
    14101 tatgggcaac acctgaagga catgcggtta ctgcaataat tttcgtcatg gctcgttcct
    14161 caattaatta atttcaaaat ccaaatccag gtcgtcttct ttttcatcag tcgaagaccc
    14221 atttttacgc gccagacttt tcagcacgtt cacacaaaca gccgtcacca ctgctccgac
    14281 agccactgct gcgatataac ccaacttacc ttcaaccacc ggcagcacaa tcagaccacc
    14341 ccagcctgca tagcactgcg caccgaccag cgccgcagtt actgcaccac aaactgaacc
    14401 gaccataatg gaaggaatga cacgcagcgg atcggcagcg gcgaaaggaa tcgccccttc
    14461 agtaacgcca acacagccca tcaccagtgc cgctttgccg gtttcgcgct cttctgcgga
    14521 gaaattttta cgaccaatca gcgtcgccag ccccatcccc agcggcggga tacaaatacc
    14581 cacggcagca atggccacca cggtataaac accctgagca acgcaaatca gcatgaaggc
    14641 ataggccact ttgttaaccg gaccgcccat atcgaacgcc agcatcagcc ccatgatcac
    14701 cgccagcata acaatgctgc cctgctgcat cccctgaagc cactgagtca ggctgttggt
    14761 caacgcccct acaggctcgc ccaagcccca catcatgatg cctgcggtaa tcagtgtgcc
    14821 gatgatagga ataatgaaga tgggcatcac cgagcgcaga actttatgca ccggaatttt
    14881 cttcaggtaa tgcaccacga tgccgccgat aatcccggcg atcagcgcac caaagaagcc
    14941 cgcaccaaag ctgttaccaa cccaggcacc gatagcgcac ggagccagcg cagaacgctc
    15001 tgcaatggag taaccaatgt aagcggcgag gaaaggcacc atcagcgtca aacctgcaac
    15061 gccgatatca aacagttttt tcaaattcgg atcggctacg gcatccggca ctgcgccttt
    15121 gccatacaac atgacggaaa ccgccagcaa aataccgccc gataccacga agggaatcat
    15181 gtgtgaaacg cccgtcatta aatgctgacg ggtatttttc aggatctgca ccaactcatt
    15241 cataagctgc tcccgggctt gcaaaagccc atgtccatat ttttgatgga ggaaacaata
    15301 ggcaatcgca ggcggagcaa acagtggata aaaaggccat ttactggact tttgtaatat
    15361 cagtacaaaa atgcgatccg cctcataact tgcgataatc cggaagcatg ccgcaaaacg
    15421 gcgtcttgtg agacgcgtcg caaattgacg gttacattac atttgtccag cttttggcag
    15481 atttgtcaca tggcaggccg ttaccgtgcg ctcttatcct gtaaacagaa aaccgtaccg
    15541 ggagaaaggc aatggccctg attgtggaat ttatttgtga gctacctaac ggcgtacatg
    15601 cgcgtccggc aagccacgtt gaaacgctgt gtaatacttt ttcatctcaa attgagtggc
    15661 ataacctgcg tactgaccgt aaaggcaacg ccaaaagcgc tctggcgctg attggcaccg
    15721 atacgctggt gggtgataac tgccagctac tgatttccgg ggccgacgaa caggaagggc
    15781 accagcgttt aagccaatgg ctgcgcgatg aattccccca ctgcgatgcg ccgctggcgg
    15841 aagttaaatc tgacgaactg gaaccactgc cggtttcact gaccaatctg aatccgcaaa
    15901 ttatccgcgc ccgcaccgtg tgcagcggta gtgcaggcgg cattctgacg ccgatctctt
    15961 ctttagatct caatgcgctg agtaatcttc ccgcagccaa agacgttgac gccgagcaat
    16021 ccgcactgga aaacggcctg acgctggtac tgaaaaacat tgagtttcgt ctgctggata
    16081 gcgacggtgc taccagcgcg attctggaag cgcaccgatc cctggctggc gatacttccc
    16141 tgcacgaaca tttactggca ggcgtcagcg ccggattaag ctgcgccgaa gcgattgtcg
    16201 ccagtgcgaa tcacttttgc gaagagtttg cccgttccag cagcagatac ctgcaagaac
    16261 gtgccctgga cgtacgcgac gtctgcttcc agttactcca gcaaatctac ggtgagcaac
    16321 gcttcccggc accgggcaaa ctgacgcagt ccgccatttg tatggctgat gaactgaccc
    16381 ccagccagtt cctcgaactg gataaaaatc acctcaaagg attgttactg aaaagcggcg
    16441 gcaccacctc acatacggtg atccttgccc gttcgttcaa cattccgacg ctggttggtg
    16501 tggatattga tgccctcact ccgtggcagc atcaaacgat ttatatcgac ggcaacgccg
    16561 gggcgattgt ggttgagcca ggagaagccg tagctcgtta ttatcagcaa gaagcccgcg
    16621 tacaggacgc cctgcgtgag caacagcgtg tctagctgac ccaacaagcc cgtaccgctg
    16681 acggtatccg cattgaaatt gccgctaaca tcgctcactc cgtggaagcg caggccgcat
    16741 tcggcaatgg tgcggaaggc gttggtttgt tccgcactga aatgctctat atggatcgca
    16801 ccagtgcacc gggcgaaagc gagctgtaca acattttttg tcaggcgctg gagtctgcca
    16861 acggacgcag cattattgtg cgcactatgg acattggcgg cgacaaaccc gttgattatc
    16921 taaacattcc cgcagaggca aacccgttcc tcggttatcg cgccgtgcgt atttatgaag
    16981 aatacgcgtc gttatttacc acacaactac gctcgatcct gcgcgcctcc gctcacggca
    17041 gcctgaaaat catgatcccg atgatctcct caatggaaga gatcttatgg gtgaaagaaa
    17101 aactggcaga agccaaacag caactacgta acgaacacat tccgtttgat gagaagatcc
    17161 agctcggcat tatgctggaa gtgccgtcgg tgatgttcat catcgatcaa tgctgcgaag
    17221 agattgattt ctttagtatt ggtagtaatg acctgacgca atatctgctg gcagtggatc
    17281 gcgataacgc taaggttact cgtcactaca acagcctgaa tccggcattc ttgcgggcgc
    17341 tcgattacga cgtgcaggcg gtgcatcgcc agggtaaatg gattggtctg tgcggtgagc
    17401 tgggagcgaa aggttccgtg ctgccgttgc tggtcggctt agggctggat gaactcagca
    17461 tgagcgcacc atcaattccg gcggcgaaag cgcggatggc gcaacttgat agccgtgagt
    17521 gccgcaagtt gctcaaccag gcaatggcct gccgtacttc gctggaagtg gaacacctgc
    17581 tggcgcaatt ccgcatgacc caacaggacg caccgctggt caccgccgag tgcatcacgc
    17641 tggaaagtga ctggcgcagc aaagaagaag tgctcaaagg catgaccgat aacctgctgc
    17701 tggcgggccg ctgccgctat ccgcgtaaac tggaagccga cttatgggcg cgcgaggccg
    17761 ttttctctac cggtctgggc tttagttttg ccattccaca cagcaaatca gaacacattg
    17821 agcaatccac catcagcgtg gcgcgtctgc aagcgccggt gcgctggggc gatgatgaag
    17881 cgcaattcat cattatgtta accctgaaca aacacgctgc gggcgatcag cacatgcgca
    17941 ttttctcgcg cctcgctcgc cgcatcatgc acgaagaatt ccgtaacgcg ctggttaacg
    18001 ccgcctctgc cgacgctatc gccagcctgc tgcaacatga actggaactg taaaaggaaa
    18061 catcatggaa ctgtatctgg acaccgctaa cgtcgcagaa gtcgaacgtc tggcacgcat
    18121 attcccgatt gccggggtga caactaaccc gagcattatc gctgccagca aggagtccat
    18181 ctgggaagtg ctgccgcgtc tgcaaaaagc gattggtgat gagggcattc tgtttgctca
    18241 gaccatgagc cgcgacgcgc aggggatggt ggaagaagcg aagcgcctgc gcgacgctat
    18301 tccgggtatt gtggtgaaaa tcccggtgac ttccgaaggt ctggcagcaa ttaaaatact
    18361 gaaaaaatag ggtattacta cacttggcac cgctgtatat agcgccgcag aagggttatt
    18421 agccgcactg gcaggggcaa aatacgttgc tccgtatgtt aaccgcgtag atgcccaggg
    18481 cggggacggc attcgtacgg ttcaggagct gcaaacgctg ttagaaatgc acgcgccaga
    18541 aagcatggtg ctggcagcca gctttaaaac gccgcgtcag gcgctggact gtttactggc
    18601 aggatgtgaa tccatcaccc tgcccttaga tgtagcgcaa caaatgctca acacccctgc
    18661 ggtagagtca gctatagaga agttcgaaca cgactggaat gccgcatttg gcactactca
    18721 tctctaaagg agcaattatg gaccgcatta ttcaatcacc gggtaaatac atccagggcg
    18781 ctgatgtgat taatcgtctg ggcgaatacc tgaagccgct ggcagaacgc tggttagtgg
    18841 tgggtgacaa atttgtttta gtttttgctc aatccactgt cgagaaaagc tttaaagatg
    18901 ctggactggt agtagaaatt acgccgtttg gcggtgaatg ttcgcaaaat gagatcgacc
    18961 gtctgcgtgg catcgcggag actgcgcagt gtggcgcaat tctcggtatc ggtggcggaa
    19021 aaaccctcga tactgccaaa gcactggcac atttcatggg tgttccggta gcgatcgcac
    19081 cgactatcgc ctcgaccgat gcaccgtgca gcgcattgtc tgttatctac accgatgagg
    19141 gtgagtttga ccgctatctg ctgttgccaa ataacccgaa tatggtcatt gtcgacacca
    19201 aaatcgtcgc gggcgcacct gcacgtctgt tagcagcggg tatcggcgat gcgctggcaa
    19261 cctggtttga agcgcgtgct tgctctcgta gcggcgcgac caccatggcg ggcggcaagt
    19321 gcacccaggc ggcgctggca ctggctgaac tgtgctacaa caccctgctg gaagaaggcg
    19381 aaaaagcgat gcttgccgcc gaacagcatg tagtaacccc ggcgctggag cgcgtgattg
    19441 aagcgaacac ctatttgagc ggcgttggtt ttgaaagcgg cggctggcgg cggctcacgc
    19501 ggtgcataac ggcctgaccg ctatcccgga cgcgcatcac tattatcacg gtgaaaagtg
    19561 gcatttggta cgctgacgca gctggtactg gaaaacgcgc cggtggagga aatcgaaacc
    19621 gtagctgcac ttagccatgc ggtaggtttg ccaataactc tcgctcaact ggatattaaa
    19681 gaagatgtcc cggcgaaaat gcgcattgtt gcaaaagcat cttgtgcaga aggtgaaacc
    19741 atccacaaca tgcctggcgg cgcgacgcca gatcaggttt acgccgcact gctggtagct
    19801 gaccagtacg gtcagcgttt cctgcaagag tgggaataac ctactccaaa ctcccggctt
    19861 gtccgggagt ttgaacgcaa aattgcctga tgcgctacgc ttatcaggcc tacgcaatct
    19921 ctgcaatata ttgaatttgc gtgcttttgt aggccggata aggcgttcac tccgcatccg
    19981 gcactctgtg ccagtaatct gaaactccca cgttgttcag gagtttttta tccgcttctg
    20041 gacattttct ccacagaaca atcgttagcc cggaaggtcg aatctgtgac tcttattttc
    20101 acggacgaac cagatgaaag cttcactggc gctgctcagc ctgctaacgg catttaccag
    20161 ccactcctta aaatctcccg ccgttccacc aaccgtggta caaatccagg ccaataccaa
    20221 ccttgccatc gcagacggtg ccagacagca aattggcagc acgctatttt acgatcgcag
    20281 cttacctatc ctggcggtga tgttccgcaa gaacgcggtg tatgttccga tgtggtgatc
    20341 cgcgcattgc gcagccagaa ggtcgatttg cagaaactgg ttcacgaaga tatggcgaag
    20401 aattttgctg agtacccgca aaaatggaag ttaaagcgcc cggacagcaa catcgatcac
    20461 cgtcgggtgc ctaatctgga aacctggttt agccgccaca acaaaacacg ccccatcagt
    20521 aaaaatgccg gtgactatca agcgggcgat attgtctcct ggcgattaga taacgggctg
    20581 gcgcatattg gggtggcgtc agatggcttc gcccgcgacg gtacgccgct ggtgatacat
    20641 aacattggcg caggcgcgca ggaagaagat gtgctgttta gctggcggat ggtggggcat
    20701 tatcgttatt ttgttaaata agagatcgcc ccacatgaag aaaaaattca gatgcttttt
    20761 acactgacag cttctttttt cttacggctc accagcgcca gtgccagcac aatcagcaca
    20821 ataccgctac cttcaacggc ccccggattc tcaccgagca gccaccagga aaagagtacg
    20881 ccacaaacgg gaacggccag tgtgcttaag ctggcaatac tggcaggcaa gtttttcagc
    20941 acaaacagcc ataagctcca cgctagcgcc gtcgccagaa tcgcactgta ggccagcgcc
    21001 cagaacacgg tgggctgcca gtcaatttca cgttgcggca ccagtaaagc gaccacactc
    21061 atcaccagcg ccgcatacag catctgccag gatgttaacg ataataaatc cacgcgcgga
    21121 tgacgggcgt ataggcgttt agcaacaatc gcgctcgccc cccaactaac accggagagg
    21181 attgccagca tggcactttt catcgaagag aaatcgagtt gccacggctg caacaccaaa
    21241 aataagccga aagcggcaat cagaatcgcg aaatattgcc cacgtcgcag gcgttcaccg
    21301 agaaacaacg cggcgaaaat caccacccag aacggcatgg tatagctcag gatcgccacc
    21361 ttccccgcac ctccgctgac caacgcccac tgcgccagac caaccatccc gcaggtttgt
    21421 aacagggcaa tggctaaggt gtatttaaac ggcgtcgggc gcattccgcg accacgtaat
    21481 aaaaggacga tgaataaaac gagagcgccg aaaatgcagc gtaaggcggt aaagtcgaag
    21541 gcaccgatgt aactggtgac ttgcttcatg aaaatccagc tataactcca gataagtgtg
    21601 agcacaacca agccactgat tgccagtggg ttgctctttc ctgcggcaga catgagagat
    21661 ccagtgtgta gttttcctgt ggacactata cgccggtaaa gaaacttcag cgactgcctg
    21721 aaagacagtc gctgaagaag gtcagattac agcaggtcga aacggtcgag gttcatcact
    21781 ttcacccatg ccgctacgaa gtctttaaca aacttctcgt gggcatcgct gctggcgtaa
    21841 acttccgcta ctgcacgcag gacagagtta gaaccaaata ccagatccgc acggctggct
    21901 gtgtatttca cttcgccggt ttcgcggtca cgtccttcga acagctcttt cgattcgtca
    21961 gtcgctttcc actcgtaacg catatccagc aagttcacga agaagtcatt gctcaatacg
    22021 ccaacgcggt cagtgaagac gccgtttttg ctgccatcga agttggcacc cagtacacgc
    22081 atcccgccca ccagcgcagt catttccggc gcggtcagcg tcagttgctg tgctttgtcg
    22141 atcagcagtg actcggtggt ggaaacgtcc agacgagcgc gatagttacg gaaaccgtca
    22201 gcaattggct ccagcagctc aaacatctca atgtcagtct gatcctgacg cgcatcaacg
    22261 cgacccggcg caaacggtac atgaatgctc aaacctgcgg cgcttgcggc tttctcaaca
    22321 ccaaccacac cagccagcac gatgatatcc gccagcgagg ctttaccaga ctctttctgg
    22381 attttctcca gaacaggcag agcacgaacg gctgcggcgt tcacatccca gtcgcgctgc
    22441 ggcattaatg ccagacgcgc accgttggca ccaccgcgtt tgtcgccacc acggaaggta
    22501 gaagcagatg cccaggccac cgataccagc tcactaacag acagaccaga atccgcaatc
    22561 gcgaatttca gatcgataat gtcctgctcg gtcgggttgt agatcggctg cggcagcgga
    22621 tcttgccaga tcagatcttc tttcggcact tccggcccga tgtagcgaga tttcggcccc
    22681 atatccctgt gcgtcagttt gaaccaggca cgggcaaagg cttcgttgaa cgcctgcgga
    22741 tcgttgagga aacgacgaga gatcttctcg aactcaggat caaaacgcag cgtcaggtcg
    22801 gtcaccagca ttgtcggttt acgtttcttc gacggatcga acggatccgg gataatttcc
    22861 ggtgcgtcta ccgcttcgaa ctggattgct ccagccggac tgcgggtctg tacccactca
    22921 tacttgaaca gattctcgaa gaaatagttg ctccactgga tcggcgtctg ggtccagact
    22981 acttccagac cagaggtaat ggcatctgcg ccaacgccgc tgccgtaagt gctcgcccag
    23041 cctaaacctt gttcttcaat cggtgcagct tctggatcag gacctacgtt tgatgtcgga
    23101 ccggcaccgt gggttttacc cagcgtatga ccacccgcaa tcagcgccac ggtttcttcg
    23161 tcgttcatgc ccatgttgcc gaaggtcgcg cggatagctg ctgccgcaga aagcggttcg
    23221 ccgctgtgat ccgggccttc cgggttaacg taaatcaggc ccatctcggt tgcacccagc
    23281 ggtgctttcg ccagcgcttc cggatgacgg tgagtcagcc aggctttttc atcaccccag
    23341 ttaacatcca gatccggttc ccagacgtct tcacgaccgg caccaaaacc gaaggtacgg
    23401 aagccggagt tttccagcgc cacgttacct gcgaggataa acaggtcggc ccaggagatt
    23461 ttctgaccat atttctgttt gattggccac aacaggcgac gcgctttatc gaggcttacg
    23521 ttatccggcc aggagttcag cggtgcaaaa cgttgctgac cacgacccgc gccgccgcgt
    23581 ccatcgattg aacggtaagt acctgcgccg tgccaagcca tacgaataaa cagaccggcg
    23641 tagctgcccc agtcggctgg ccaccacggc tgagattctg tcagcagggc tttcagatct
    23701 tttttcaggc cgtagtaatc taatttgctg aattctttgc ggtagtcaaa gtcctcaccc
    23761 agcgggttag aacgattaga atgttggttt aacaggtcaa cacggagttg atttggccac
    23821 cagtcgcgag tggttgtgcc cgcccccgcg ctctggtcgt gaccgccctg atggaacggg
    23881 catttgccag tggctgtggt gttatggata tcgtctgacg tgctcatcaa tgtgctcccc
    23941 tctacagtgt taccgttacg atacacagcg ttagagagaa gttataattg aattaatcca
    24001 cggatgcgat agttgagatc ttacatattg ttggttaaag agatgtagat caaattgatc
    24061 gtaattaagc ttttttgatt atgtccgaat tcggacaaat gctttataaa aaagggtttt
    24121 agcttcatat ccttcaagta atgagggaga aaacaaggct gacaacctta ttttcccgcc
    24181 ctcatttcga ggcagcattt tgtgctctgt ttaaaatttg tgatcactgt gtgattttca
    24241 caaaagccac actatttata aaccaggtcg aacacccagc gtatggcaaa tcgcgtaact
    24301 catttcagcg cggttaagcg tatagaagtg gaaatctttc actccttcac ggcttaaaat
    24361 cttcaccata tccatggcaa tattcgcgcc caccagtttg cgggtttcgg catcgtcatc
    24421 cagcccgtca aacatttgcg ccatccacgc cggaatacgc acgttggtca tgtcggcaaa
    24481 tttcttcgcc tgtttaaagt tagataccgg caaaatgccc gggatgattt ccacatcaat
    24541 gcccgccgat acacagcggt cacgaaaacg caggtagctt tcgacatcga agaagaactg
    24601 agtaatcgcg cggttggctc cggcatccac tttgcgtttc agattaagca aatccgcctg
    24661 agcgcttttt gcttccgggt gaacttccgg atacgccgcc acggatatat cgaaatctgc
    24721 cacttctttt aacagcgtca ccaggtcaga agcatacatt tctggcttac cacttcccgg
    24781 cggcagatcg ccacgcagtg ccacgatatg acgaataccg ttattccagt agtcgcgtgc
    24841 aatggtgcgc agctcgtcgg gcgtcgcatc aatgcaagta agatgcggtg ccgcttccag
    24901 accagtgcga tctttaatgc ctttaataat gctgtgcgta cggtcgcgct cgccggagtt
    24961 cgcgccatag gtcaccgata caaacttcgg tttcaggctg ctaaggcgat cgatggagtt
    25021 ccacagggtc tgctccattt cactggtacg cggcgggaaa aactcgaacg aaacgttaat
    25081 ctgcccctgg acttctgcca ggctctgatt cagggcatcc cgctggctgg cgtgaaaaaa
    25141 gctcatacct tacctcatca atcgcttgta gttatatgtt gtgttgtgca catctatcca
    25201 tttagacgtc cagatgtaaa gatgaaggaa aagccgaagg gcgtcaaccg aaaaatgacc
    25261 aatgatggtg aggaaaaatc agggaagatg aaaaaacttc aggatggtaa gaaaaagaaa
    25321 atccgatatg caaagcaaac cggactgcaa atataaacta accatgtact tattgcttct
    25381 tgccggattc gtcgtaaacg cttcatccgg cctaattcta tgataattca atatatttca
    25441 ttagaaacca tagacccgaa aacatgagta ccgggcatta ttaaatttct gaaattacaa
    25501 caactgtgcc agccggttga tatccgactg aatcgccccg gcggtgacgt cgcgcccagc
    25561 gccaggccca cggatcacca gcggattatc gcggtaccag cggctttcga tggcaaagac
    25621 gttatcgcat ggcagcagtg atgccagcgg atagtcttca cgcaccgcct ccacgcccac
    25681 acgcgctttg ccgttggcat cgaaacgcgc cacgtagcgc agcaccagcc ccatttcgcg
    25741 ggccgcttcc agccgttgca ccatctgctc gttaagttca tcgccatttt caaagaaatg
    25801 gtcgatgctg ccgccttcgc aatgagcagg caccagcgat tccacacgta cctggtccgg
    25861 ttcaatgttg taacccgctt cacgcgccag aatcaccagc ttgcgcatta cgtctttgcc
    25921 ggagaggtcg tcacgcggat caggctcggt taagccctgc tgccacgcct gatccaccag
    25981 ctcggtaaac ggcacgctac cgtcgaattg caggaacagc caggagagcg tgccggagaa
    26041 gatcccactg atcgacaaaa tggtatcgcc gctgtcgatc agatcgcgca cggtatggtt
    26101 gatcggcaag cccgcaccaa cggtggcgtt gtatagccag tgacgcccgg ttttttcgaa
    26161 ggcgtcgtgg atctggcgat atttattgct gtcgcttgct cccgccagtt tgttggcact
    26221 gatgacgtgg aaaccgtggc tggcgaaatc gagatactgg tcagcaagct gctggctggc
    26281 ggtaacgtcc aacaccacta aatcatcata cggatgggcg cgcatccaca ggaaaagcga
    26341 ctcttcatcc tgctcgaccg cttcatcgtt gaagaaggct aacgcgcggc tggcgtccag
    26401 cccgtcataa ctcaacagac tgcggcggct gtccaccacg cctgccagca caaattcaaa
    26461 gccggtacgc gccgaaagcg tgctctgctc gcgggcgaac agttccagcc agcgagagcc
    26521 gatattgcct ttgccgaaca ataccaggcc gatgcgtttt tctgcgcgga agacggactg
    26581 atgcagcccc tgaatcaggc tttcggtcgg gccggtgcgc agtactgcca ccagactgat
    26641 gccgtcatcg gactgccacg taaactcgac cggctggcct ttcagttgct gccagaagcg
    26701 gtggcaatgc agcgggttac gggtgacacc cgccccgacc atcgccacca gcgccagccc
    26761 ctgacgcagg cgcagttctc caggtaatcc cgcttcgtcg aggattttca gcgcactgtc
    26821 ggccacttct gaggtgtagc aaaattgcaa caactggcga tcgttatgca cgccaaccgc
    26881 cagtggacgc acctgggcac gtttcaggat ctggtcgatc tctttatgcg ccagtttgaa
    26941 atcctgactg gcgggcacct gaaactcaat caaacagaca tcatcgtggc tggtgacaat
    27001 acgcgcacca gtaccggagg ccagcacgcg ttcaatgcgc gtggaacctt gatccggcgt
    27061 gtagctacag cgcagctgca ggtcgatttc gctgccagaa actggctgta aagtacgggc
    27121 gtgaagaacg ggagccgcca gacgcgccag ttcgctggcc tcatccagac gcagcaacgg
    27181 cagcaggcag gcatctttca ctttacgcgg gtcggcactg tataccccgg cgacgtcgct
    27241 ccagatggtt acacgagaaa cacccgccag cgcaccgatt tgtgtcgcgg aatagtcgga
    27301 accgttacgc cccagcaaca ccgtttcacc ggcgttgtta cggctgataa acccggtcac
    27361 caccagacgt ttgctcggat gttgtaccag tagctgttgc agcaacgggt aagaaagccc
    27421 ttcatcaacc tgcggttgcg cagcgcgttc agcgcgtaaa aactcacggg catcaagcca
    27481 ggccgctggc agcccttgtt gattaagtac cgcagacatc aggcgcgccg accacacttc
    27541 accgtggccc accacttccg catacaccgc atcgttaata ccactgtcga gcaacgccgc
    27601 caggcgctca agatcgctga caaaagcgct aatgaggcta tcggcttctt cagcgggtag
    27661 cagaccgcta atcagatcgc actgatagcg acgcagcgtt tgttgaacct gatgcgcaga
    27721 gagacgatcg gtctggctta gtttcaacca gttaatcaac tggttagtgg tgctaccggc
    27781 ggcggaaacc accatcatat cgtcaggctg agagaactcc gccataatgc ccgcgacacg
    27841 caaataacac ttcacatcag ccagactact gccaccaaat ttatgcagct gacgaccttt
    27901 cgcccctgcc tgcgcaatca cactcatttt taccccttgt ttgcagcccg gaagccattt
    27961 tccaggtcgg caattaaatc ttcgccatct tcaataccgg tggagatacg cagcagcgtc
    28021 tcggagatcc cggcggcagc acgcgcttct ggtgccatgc ctgcatgtgt catggttgcg
    28081 gcgtgagaga ttaaactttc cactccccct aatgattccg ccagcgtaaa caacgacagc
    28141 ccgcccagga aacgacacag cgtctgctca tcgccatcca gttcaaaact caacattgcg
    28201 ccaaagcctt tttgctgacg cgcggcaatt tcatgcccct gattttccgg cagcgacggg
    28261 tgatacagtt ttttcaccaa cggctgggtt tgcagatatt tcacaatcgc ctgcgcgttg
    28321 cgctgcgcca gctccatacg cggcaccagc gttcgcaacc cgcgtagcag cagatagctg
    28381 tcaaacgcgc cgcccgtcac gccaatattg tttgcccacc aggcaagttc agtgacaacg
    28441 tccgggtctt tagcaatcac cacgccagcc accacgtcag agtgaccgtt cagatatttc
    28501 gtacatgaat gcaacaccag atcggcaccc aatgccagcg gattttgtaa tgccgggctt
    28561 aagaaggtgt tatccaccac gctcaccgcc ccgacttccc ttgccagatg gcagattttc
    28621 gcaatatcca cgacgcgtaa caatggatta cttgggcttt ctaccagtac cagtttgggt
    28681 ttttctgcca gcgctgcccg taatgcctgt tcatcgcctt gatcaacaaa caacacgcga
    28741 tagcaaccgc gtttcgccag actgtcgaac aggcgatagc taccgccgta gcagtcgtgc
    28801 ggcgcaacca gcagatcgcc aggtttcaaa aagacggtcg ttaccaggtg aatcgcggac
    28861 atgccggtat tagtaagtac tgcgccagca ccgccttcca gttctgccag cgcacgctga
    28921 accacatcgc gcgttgggtt gccgcgacgc gagtaatcat gcgcgcgcgg ttcattaaat
    28981 ccggtaaagt tataggtgct ggaaagatgg atgggaggga caacgcaacc atactgttcg
    29041 tcgtcattta acccgctacg cactgcgatg gtggcctgtt tacgcgtcat gtgatgaagt
    29101 ttcctgggct ttgtcggtga aatgtcaggc accagagtaa acattgtgtt aatggacgtc
    29161 aatacatctg gacatctaaa cttctttgcg tatagattga gcaaatccca aatagccgtt
    29221 aaaattatat gcattatcac gccgacaggt gcattacacg atgtcacggt aacgcctgta
    29281 cggtaaacta tgcgggttta cggtcagtac ccacataaat tgtggggtct ggtctcaatt
    29341 tattgacgaa gaggattaag tatctcatgg ctgaatggag cggcgaatat atcagcccat
    29401 acgctgagca cggcaagaag agtgaacaag tcaaaaagat tacggtttcc attcctctta
    29461 aggtgttaaa aatcctcacc gatgaacgca cgcgtcgtca ggtgaacaac ctgcgtcacg
    29521 ctaccaacag cgagctgctg tgcgaagcgt ttctgcatgc ctttaccggg caacctttgc
    29581 cggatgatgc cgatctgcgt aaagagcgca gcgacgaaat cccggaagcg gcaaaagaga
    29641 tcatgcgtga gatggggatt aacccggaga cgtgggaata ctaaagcgga aaagagcggc
    29701 gcggagtgga atcgcctgat gcactacgct tatcaggcct acgtcatatt gcaatttatt
    29761 gaatttgcac gaacttgtag gccggataag gcgttcacgc cgcatccggc ataaacaacg
    29821 agcacgttgt ctgcgaccca ccgcttttta tacatggacg tttaactatg aaaaacaggc
    29881 tgctgatcct cagcctgctg gtttctgtac ctgcctttgc ctggcagccg caaactggcg
    29941 acatcatctt tcaaatctct cgctcatcgc aaagtaaagc gatccaactg gcaacccatt
    30001 ccgattacag ccacaccggt atgctggtga tgcgtaacaa aaagccctac gtttttgaag
    30061 cggtcggccc ggtgaaatac accccgctca agcagtggat tgcccatggt gaaaagggca
    30121 actacgttgt tcgccgcgtt gaaggcggac tgagtgttga acaacagcaa aaactgacgc
    30181 aaacggcaaa acgttatctc ggtaaaccgt atgatttcag cttctcatgg agcgacgatc
    30241 gccagtactg ttcggaagtg gtgtggaagg tttaccagaa cgcgctggga atgcgcgtgg
    30301 gcgaacagca gaagttgaaa gagtttgatc tcagcaaccc gctggtccag gcgaagctca
    30361 aagaacgcta cggtaaaaat attccgcttg aggaaacggt agtttcacca caagccgttt
    30421 tcgacgcgcc acaactcacc acggttgcca aagagtggcc gctgttttcg tggtgatacg
    30481 accaaaattc aggcacaaaa aaagcgccgt gcggcgcttt tttcggaaat ccggtcttat
    30541 ttgctgcccg ggatgttgaa acgcttgttg aagcggtcaa cacggccacc ggtagcaaca
    30601 tcacgctgtt tgccagtgaa gaacgggtgg cacttgctgc acacgtcgag gttcaggtca
    30661 tgaccaacgg tggagcggat ttttattacg ttaccgcaag agcagctagc agtaatttct
    30721 tcgtatttcg ggtgaatatc ttttttcatg ggaaaacctc ggtttaaggc cgcgtcgctc
    30781 ttccagccct aacgccagac accacgcgat gttaaaagta tagcttcaat acgatcattt
    30841 cgtacgaagg cgcgaaatca tacagaaatt aaccagcgta tgcaaactga tccgcactct
    30901 tctacggcaa tgtgtatact aacccaccga atttcaagtc aggatgatgc tatgcccgtt
    30961 gcccacgttg ccttgcccgt tccgcttcct cgtacctttg actatctgct gccagaaggc
    31021 atgacggtta aagctgggtg tcgcgtgcgc gtgccgtttg gcaaacagca ggagcgcatc
    31081 gggattgtgg tatcagttag cgatgccagc gaactgccgc tcactgagct aaaagcggta
    31141 gtcgaagtgc tggatggtga gccggtgttt actcactccg tctggcgatt gctgctatgg
    31201 gcggcagatt actatcatca tccgattggc gatgtgctgt ttcatgcctt gccgatttta
    31261 ctacgccagg ggcggcctgc ggcgaacgcg ccgatgtggt actggtttgc cactgaacaa
    31321 ggtcaggcgg tggatctgaa cagcctgaaa cgctcaccca agcaacaaca ggcgctggca
    31381 gcgttacggc aaggcaaaat ctggcgcgac caggttgcca cgctcgaatt taatgatgcc
    31441 gcgttgcagg cgctgcgcaa aaaaggtctg tgtgatttag caagtgaaac accagagttt
    31501 agcgactggc gaacgaacta tgccgtttct ggtgagcggt tgcgattgaa taccgaacag
    31561 gccaccgccg ttggcgcaat tcatagcgcg gcagatactt tttctgcctg gctgctggcg
    31621 ggcgttaccg gttccggtaa aacggaggtt tatctcagtg tactggaaaa cgtgctcgct
    31681 cagggcaaac aggcgctggt gatggtgccg gaaatcggcc tgacaccgca aactatcgcc
    31741 cgttttcgtg aacggtttaa tgcgcccgtg gaagttctgc attccggtct gaacgacagc
    31801 gagcgtcttt cggcgtggct gaaagcgaaa aatggcgagg cggcgattgt gatcggcacc
    31861 cgctccgcgc tgtttacgcc gtttaaaaat ctcggcgtga ttgtcattga cgaagagcac
    31921 gacagttcct acaagcagca ggaaggctgg cgctatcatg cccgcgacct ggcggtgtat
    31981 cgtgcgcata gcgagcaaat cccgattatt cttggctccg caacgcccgc gctggaaacg
    32041 ttgtgcaacg tccagcagaa aaaataccgc ctgctgcgcc tgacgcgccg ggcggggaat
    32101 gcgcgtccgg caattcaaca cgtgctggat ttaaaaggtc agaaggtgca ggcaggtctg
    32161 gctccggcgt taatcacccg tatgcgccag catttacagg ccaacaatca ggttatcctc
    32221 tttcttaacc gccgtggctt tgcgcctgcg ctactgtgcc acgactgcgg ctggattgcc
    32281 gaatgcccac gttgcgatca ctactacacg ctgcatcagg cgcaacagca tttgcgctgt
    32341 caccactgtg acagtcagcg tccggtgccg cgccagtgcc cttcctgcgg ttccacgcac
    32401 ctggtacccg tggggctggg caccgaacag cttgaacaga cgcttgcgcc gctgttccct
    32461 gatgtgccca tttctcgtat cgaccgcgat accaccagcc gcaaaggggc gctggaacag
    32521 caactggcgg aagtacatcg cggcggtgcg aggattttga tcggcacaca aatgctggcg
    32581 aaaggtcacc actttccgga tgtgacgctg gtggcgttac tggatgtgga cggcgcgctg
    32641 ttttctgccg attttcgctc ggcagagcgt ttcgctcagc tttacaccca ggtcgctggt
    32701 cgtgccgggc gtgcgggtaa acaaggcgaa gtggtgctgc aaacgcacca tccggaacat
    32761 cctctgttgc aaacgttgct ctataaaggc tacgatgcct ttgccgaaca ggcgctggct
    32821 gagcgacgga tgatgcaact gccgccatgg accagccatg tgattgtgcg tgcggaagat
    32881 cataacaatc agcatgcgcc gctgttcctg caacaactgc gtaatctgat cctctccagc
    32941 ccgctggcag acgacaaact gtgggttctc ggtccggttc cggcactggc acctaaacgt
    33001 ggcggtcgct ggcgctggca gatattgttg cagcaccctt cccgcgtgcg cttgcaacac
    33061 atcattagtg gtacgctggc gctcatcaat acaataccgg attcccgcaa ggtgaaatgg
    33121 gtactggatg ttgatccgat tgagggttaa accgcttacg atgcgaggcg gatcgaaaaa
    33181 ttcaatattc atcacacttt tcatgaaaat tctgtaaccg ttttcacgcg ctatctgcta
    33241 aaaatgttgc cgatgtgaag taaacatgga tgtagtacgc ctgacgtgcc aggcgaggag
    33301 tgagtgtgaa agcgaagaag caggaaactg ccgcgaccat gaaagacgtt gccctcaagg
    33361 caaaagtctc tacagcgact gtctcccgag cattaatgaa tcccgataaa gtctcccagg
    33421 ccacccgtaa tcgggttgaa aaagcggccc gggaagtggg ttatttaccg cagcctatgg
    33481 ggcgcaacgt caagcgtaat gaatcccgca ccattctggt gattgtcccg gatatctgcg
    33541 atcccttctt tagcgaaatt attcgcggta tcgaagttac ggcggcaaat cacggatatc
    33601 tggtgctgat tggcgactgt gcgcatcaaa atcagcagga aaaaaccttt atcgatttga
    33661 tcatcaccaa gcaaattgat ggcatgttgc tgctgggttc aaggctgccg tttgatgcca
    33721 gcattgagga acagcgtaat ctgccgccga tggtgatggc gaacgaattt gcaccggaac
    33781 tggagctgcc taccgttcat atcgacaatc tgaccgccgc atttgatgca gtaaattatt
    33841 tatatgagca agggcataaa cggattggct gtatagccgg tcccgaagag atgccactgt
    33901 gtcactaccg cctgcaaggc tatgttcagg cgctgcgtcg ctgcggaatt atggttgatc
    33961 cacaatacat cgcccgtggc gacttcacct tcgaagccgg aagcaaagca atgcaacagc
    34021 tgcttgatct tccacaaccg cctactgcag tcttctgcca tagcgatgtg atggcgctcg
    34081 gcgcactttc tcaggcaaaa cgccaggggc tgaaagtccc ggaagacctt tccataatcg
    34141 gttttgataa catcgacctg acgcaatttt gtgatccgcc gctgacaacc atcgcgcagc
    34201 cgcgttacga aatcggtcgg gaagctatgc tgttattgct tgatcaaatg caggggcaac
    34261 acgttggcag tggctctcgt ttaatggact gcgaacttat catccgggga tcaacacgcg
    34321 cgttacctta aagtaaacct ttaagactgc cgtgactggt caaaggcccg ccgcttaagt
    34381 aacatggcgg gctgacgaac gaataaatac agcgaaacga tagtggcaca acgagattat
    34441 gtacgccgca gccaaccggc accttcgcgg cgaaaaaaga gcacctcacg gaaaaagcaa
    34501 cgaaatctgc ctgcggtttc tcccgctatg gtcgctattg ctgccgccgt tcttgtgacc
    34561 tttatcggtg gtctgtactt cattacgcat cacaagaaag aagagtccga gacgctgcaa
    34621 agccagaaag tgaccggaaa cggactacca ccaaaaccag aagaacgctg gcgctacatt
    34681 aaagagctgg aaagtcgcca gccgggtgtg cgtgcgccca cagaaccttc tgccggtggt
    34741 gaagtgaaaa cgccggagca actgacacca gaacaacgtc agcttcttga acaaatgcag
    34801 gctgatatgc gccagcagcc aacgcagctg gttgaggttc cgtggaatga acagacgccg
    34861 gaacagcgtc agcaaacgct acaacgccag cgtcaggcgc agcaattagc ggagcagcaa
    34921 cgcctggctc agcagtcgcg tacgactgaa caaagctggc agcagcagac gcgtacgtcg
    34981 caagccgcgc cggtacaggc gcaaccgcgc cagtctaaac cagcttctac tcagcagccg
    35041 taccaggatc tgctgcaaac ccctgcgcac acgaccgcgc agtcgaagcc gcagcaagct
    35101 gcgccggttg ctcgtgccgc tgacgcgcca aaaccgacgg cggagaaaaa agacgaacgc
    35161 cgctggatgg tgcagtgcgg ttcgttcaga ggcgcggaac aggcagagac ggtacgtgct
    35221 cagctggcgt tcgaaggatt tgactcgaaa atcaccacca acaatggctg gaatcgtgtg
    35281 gtcattggcc cggtgaaagg caaagagaac gcagacagca ccctcaatcg gttgaagatg
    35341 gcgggtcata caaactgcat tcggctcgcc gccgggggtt gaaaccctca aaatcccccc
    35401 catctataat tgcattatgc cccgtacttt tgtacggggt ttgtactctg tattcgtaac
    35461 caaggggtca gctcgtgaca actatagtaa gcgtacgccg taacggccat gtggtcatcg
    35521 ctggtgatgg tcaggccacg ttgggcaata ccgtaatgaa aggcaacgtg aaaaaggtcc
    35581 gccgtctgta caacgacaaa gtcatcgcgg gctttgcggg cggtactgcg gatgctttta
    35641 cgctgttcga actgtttgaa cgtaaactgg aaatgcatca gggccatctg gttaaagccg
    35701 ccgttgagct ggcaaaagac tggcgtaccg atcgcatgct gcgcaaactt gaagcactgc
    35761 tggcagtcgc ggatgaaact gcatcgctta tcatcaccgg taacggtgac gtggtgcagc
    35821 cagaaaacga tcttattgct atcggctccg gcggccctta cgcccaggct gcggcgcgcg
    35881 cgctgttaga aaacactgaa cttagcgccc gtgaaattgc tgaaaaggcg ttggatattg
    35941 caggcgacat ttgcatctat accaaccatt tccacaccat cgaagaatta agctacaaag
    36001 cgtaaggatc tcccatgtct gaaatgaccc cacgcgaaat cgtcagcgaa ctggataagc
    36061 acatcatcgg ccaggacaac gccaagcgtt ctgtggcgat tgctctgcgt aaccgctggc
    36121 gtcgcatgca gctcaacgaa gagctgcgcc atgaagtgac cccgaaaaat atcctgatga
    36181 tcggcccgac cggtgtcggt aaaactgaaa tcgcccgtcg tctggctaag ctggcgaatg
    36241 cgccgttcat caaagttgaa gcgaccaaat ttaccgaagt gggctacgtc ggtaaggaag
    36301 ttgattctat tattcgcgat ctgaccgatg ccgccgtgaa aatggtacgc gtccaggcta
    36361 tcgagaaaaa ccgttatcgc gctgaagaac tggcagaaga acgtattctc gacgtgctga
    36421 tcccacctgc taaaaacaac tggggacaga ctgaacagca gcaggaacct tccgctgctc
    36481 gtcaggcatt ccgcaaaaaa ctgcgtgaag gccagcttga tgacaaagaa atcgagatcg
    36541 atcttgccgc agcaccgatg ggcgttgaaa ttatggctcc tccgggcatg gaagagatga
    36601 ccagccagct gcagtccatg ttccagaacc tgggcggcca gaagcaaaaa gcgcgtaagc
    36661 tgaaaatcaa agacgccatg aagctgctga ttgaagaaga agcggcgaaa ctggtgaacc
    36721 cggaagagct gaagcaagac gctatcgacg ctgttgagca gcacgggatc gtgtttatcg
    36781 acgaaatcga caaaatctgt aagcgcggcg agtcttccgg tccggatgtt tctcgtgaag
    36841 gcgttcagcg tgacctgctg ccgctggtag aaggttgcac cgtttccacc aaacacggga
    36901 tggtcaaaac tgaccacatt ctgtttatcg cttctggcgc gttccagatt gcgaaaccgt
    36961 ctgacctgat cccggaactg caaggtcggc tgccaatccg cgttgaactg caggcactga
    37021 ccaccagcga cttcgagcgt attctgaccg agccgaatgc ctctatcacc gtgcagtaca
    37081 aagcactgat ggcgactgaa ggcgtaaata tcgagtttac cgactccggt attaaacgca
    37141 tcgcggaagc ggcatggcag gtgaacgaat ctaccgaaaa catcggtgct cgtcgtttac
    37201 acactgttct ggagcgttta atggaagaga tttcctacga cgccagcgat ttaagcggtc
    37261 aaaatatcac tattgacgca gattatgtga gcaaacatct ggatgcgttg gtggcagatg
    37321 aagatctgag ccgttttatc ctataatcgc gttcaatcat tttcatcatt gtttgatggg
    37381 gctgaaaggc cccattttta ttggcgcgta ttatgactga acaacaaatt agccgaactc
    37441 aggcgtggct ggaaagttta cgacctaaaa ccctccccct cgcctttgct gcaattatcg
    37501 tcgggacggc gctggcatgg tggcaaggtc acttcgatcc gctggtcgcc ctgctggcac
    37561 taattaccgc cgggctatta cagatccttt ctaacctcgc caatgattac ggcgatgcgg
    37621 taaaaggcag cgataaacct gaccgcattg ggccgctacg cggcatgcaa aaaggggtca
    37681 ttacccagca aaagatgaaa cgggcgctca ttattaccgt cgtgctcatc tgtctctccg
    37741 ggctggcact ggttgcagtg gcatgccata cgctggccga ttttgtcggt ttcctgattc
    37801 ttggcgggtt gtcgatcatt gccgctatca cctacaccgt gggcaatcgt ccttatggtt
    37861 atatcggtct gggtgatatt tccgtactgg ttttctttgg ctggttgagt gtcatgggga
    37921 gctggtattt acaggctcat acattgattc cggcactgat ccttccggcg accgcatgcg
    37981 gcctgctggc aacggcagta ctgaatatta ataacctgcg tgatatcaat agcgaccgcg
    38041 aaaatggcaa aaacacgctg gtggtgcgct taggtgaagt gaacgcgcgt cgttatcatg
    38101 cctgcctgct gatgggctcg ctggtgtgtc tggcgctgtt taatctcttt tcgctgcata
    38161 gcctgtgggg ctggctgttc ctgctggcgg caccattact ggtgaagcaa gcccgttatg
    38221 tgatgcggga aatggacccg gtggcgatgc gaccaatgct ggaacgtact gtcaagggag
    38281 cgttactgac taacctgctg tttgttttag ggatattcct aagccagtgg gcagcataac
    38341 tgacaaatat caattaacaa ttgatgattt tgccaacagc ccacatagcg cgatatactg
    38401 aaaattctcg cagcaactga atgttaagcc tatgaaatac gatacttccg agctttgtga
    38461 catctatcaa gaagatgtta acgtcgtgga accgctgttc tccaactttg gcggacgggc
    38521 gtcgtttggc ggacaaataa tcacggtaaa atgtttcgag gacaacgggt tgctgtacga
    38581 tctgctcgaa cagaatggcc gtggtcgtgt tcttgtcgtc gatggcggtg gttctgttcg
    38641 tcgcgcactg gtcgatgctg aactggcgcg tctggcagta caaaatgaat gggaaggtct
    38701 ggtcatttac ggcgcggtgc gtcaggtaga tgacctggaa gagttggata tcggcatcca
    38761 ggcgatggcg gcaattccgg ttggtgccgc tggcgaaggc attggcgaaa gcgatgtccg
    38821 cgtcaatttt ggcggtgtca ccttcttctc cggcgaccat ctttatgccg acaataccgg
    38881 gattattctt tcagaagatc cgctggatat tgaatgataa gaaaggcacc gcaaggtgcc
    38941 ttttttctgc gttacctgtt ggcctacaca gtaaagaaat tacgcgaaag atgaagcgta
    39001 atcagacctc ttccatgcga cccagcaggg cctgcagacg ttcctgccag ccgttctgct
    39061 gttctttcag atggttgttc tcacgctcca gctcttcgcg ctgatgctgg gcattttgaa
    39121 cttcctgcga cagtgagttg tttttttctt tcagctcttc gatttccatc tgcaacagag
    39181 tgatggtatc aatcgcctgc tgtacttttg cttccagttt ctcaaacact tctaatgaca
    39241 ttgtcatacc tctcctgaat tgcaaggcgt tgatggataa aaaatcctcg tcccgattac
    39301 cggtgacgcc ttaataaata cgagcgcact ttagttagct ccgattgtat gaagccgcgc
    39361 catcgctgtc cagcggcacg ccttgcagat tacggtttgc cacacttttc atccttctcc
    39421 tagtgacata atccacatca atcgaaaatg ttaataaatt tgttgcgcga atgatctaac
    39481 aaacatgcat catgtacaat cagatggaat aaatggcgcg ataacgctca ttttatgacg
    39541 aagcacacac attttaagtt cgatatttct cgtttttgct cgttaacgat aagttaacag
    39601 catgcctaca agcatcgtgg aggtccgtga ctttcacgca tacaacaaac attaactctt
    39661 caggatccga ttatgagtca aacatcaacc ttgaaaggcc agtgcattgc tgaattcctc
    39721 ggtaccgggt tgttgatttt tttcggtgtg ggttgcgttg cagcactaaa agtcgctggt
    39781 gcgtcttttg gtcagtggga aatcagtgtc atttggggac tgggggtggc aatggccatc
    39841 tacctgaccg caggggtttc cggcgcgcat cttaatcccg ctgttaccat tgcattgtgg
    39901 ctgtttgcct gtttcgacaa gcgcaaagtt attcctttta tcgtttcaca agttgccggc
    39961 gctttctgcg ctgcggcttt agtttacggg ctttactaca atttattttt cgacttcgag
    40021 cagactcatc acattgttcg cggcagcgtt gaaagtgttg atctggctgg cactttctct
    40081 acttacccta atcctcatat caattttgtg caggctttcg cagttgagat ggtgattacc
    40141 gctattctga tggggctgat cctggcgtta acggacgatg gcaacggtgt accacgcggc
    40201 cctttggctc ccttgctgat tggtctactg attgcggtca ttggcgcatc tatgggccca
    40261 ttgacaggtt ttgccatgaa cccagcgcgt gacttcggtc cgaaagtctt tgcctagctg
    40321 gcgggctggg gcaatgtcgc ctttaccggc ggcagagaca ttccttactt cctggtgccg
    40381 cttttcagcc ctatcgttgg cgcgattgta ggtgcatttg cctaccgcaa actgattggt
    40441 cgccatttgc cttgcgatat ctgtgttgtg gaagaaaagg aaaccacaac tccttcagaa
    40501 caaaaagctt cgctgtaata tgactacggg acaattaaac atgactgaaa aaaatatatc
    40561 gttgcgctcg accagggcac caccagctcc cgcgcggtcg taatggatca cgatgccaat
    40621 atcattagcg tgtcgcagcg cgaatttgag caaatctacc caaaaccagg ttgggtagaa
    40681 cacgacccaa tggaaatctg ggccacccaa agctccacgc tggtagaagt gctggcgaaa
    40741 gccgatatca gttccgatca aattgcagct atcggtatta cgaaccagcg tgaaaccact
    40801 attgtctggg aaaaagaaac cggcaagcct atctataacg ccattgtctg gcagtgccgt
    40861 cgtaccgcag aaatctgcga gcatttaaaa cgtgacggtt tagaagatta tatccgcagc
    40921 aataccggtc tggtgattga cccgtacttt tctggcacca aagtgaagtg gatcctcgac
    40981 catgtggaag gctctcacga gcgtgcacgt cgtggtgaat tgctgtttgg tacggttgat
    41041 acgtggctta tctggaaaat gactcagggc cgtgtccatg tgaccgatta caccaacgcc
    41101 tctcgtacca tgttgttcaa catccatacc ctggactggg acgacaaaat gctggaagtg
    41161 ctggatattc cgcgcgagat gctgccagaa gtgcgtcgtt cttccgaagt atacggtcag
    41221 actaacattg gcggcaaagg cggcacgcgt attccaatct ccgggatcgc cggtgaccag
    41281 caggccgcgc tgtttggtca gttgtgcgtg aaagaaggga tggcgaagaa cacctatggc
    41341 actggctgct ttatgctgat gaacactggc gagaaagcgg tgaaatcaga aaacggcctg
    41401 ctgaccacca tcgcctgcgg cccgactggc gaagtgaact atgcgttgga aggtgcggtg
    41461 tttatggcag gcgcatccat tcagtggctg cgcgatgaga tgaagttgat taacgacgcc
    41521 tacgattccg aatatttcgc caccaaagtg caaaacacca atggtgtgta tgtggttccg
    41581 gcatttaccg ggctgggtgc gccgtactgg gacccgtatg cgcgcggggc gattttcggt
    41641 ctgactcgtg gggtgaacgc taaccacatt atacgcgcga cgctggagtc tattgcttat
    41701 cagacgcgtg acgtgctgga agcgatgcag gccgactctg gtatccgtct gcacgccctg
    41761 cgcgtggatg gtggcgcagt agcaaacaat ttcctgatgc agttccagtc cgatattctc
    41821 ggcacccgcg ttgagcgccc ggaagtgcgc gaagtcaccg cattgggtgc ggcctatctc
    41881 gcaggcctgg cggttggctt ctggcagaac ctcgacgagc tgcaagagaa agcggtgatt
    41941 gagcgcgagt tccgtccagg catcgaaacc actgagcgta attaccgtta cgcaggctgg
    42001 aaaaaagcgg tcaaacgcgc gatggcgtgg gaagaacacg acgagtaatg tcaggcacct
    42061 gaatcgattg cctgatgctg atcgcctgat gcgacgctta cgcgtcttat caggcctacg
    42121 agccaatgcc attttgtgta ggcgggataa ggcgttcacg ccgcatccgg catcacgcag
    42181 actctgaaac cgtgcggggg caaccccgca cacatcaata atcccaccct tcccctgtgc
    42241 tacacttcgc gccattcctt actgcttaga gtttgctatg agacgagaac ttgccatcga
    42301 attttcccgc gtcaccgaat ctgcggcgct ggctggctac aaatggttag gacgcggcga
    42361 taaaaacacc gcggacggcg cggcggtaaa cgccatgcgt attatgctca accaggtcaa
    42421 cattgacggc accatcgtca ttggtgaagg tgaaatcgac gaagcaccga tgctctacat
    42481 tggtgaaaaa gtcggtactg gtcgcggcga cgcggtagat attgctgttg atccgattga
    42541 aggcacgcgc atgacggcga tgggccaggc taacgcgctg gcggtgctgg cagtaggcga
    42601 taaaggctgc ttcctcaatg cgccggatat gtatatggag aagctgattg tcgggccggg
    42661 agccaaaggc accattgatc tgaacctgcc gctggcggat aacctgcgca atgtagcggc
    42721 ggcgctcggc aaaccgttga gcgaactgac ggtaacgatt ctggctaaac cacgccacga
    42781 tgccgttatc gctgaaatgc agcaactcgg cgtacgcgta tttgctattc cggacggcga
    42841 cgttgcggcc tcaattctca cctgtatgcc agacagcgaa gttgacgtgc tgtacggtat
    42901 tggtggcgcg ccggaaggcg tggtttctgc ggcggtgatc cgcgcattag atggcgacat
    42961 gaacggtcgt ctgctggcgc gtcatgacgt caaaggcgac aacgaagaga atcgtcgcat
    43021 tggcgagcag gagctggcac gctgcaaagc gatgggcatc gaagccggta aagtattgcg
    43081 tctgggcgat atggcgcgca gcgataacgt catcttctct gccaccggta ttaccaaagg
    43141 cgatctgctg gaaggtatta gccgcaaagg caatatcgcg actaccgaaa cgctgctgat
    43201 ccgcggcaag tcacgcacca ttcgccgcat tcagtccatc cactatctgg atcgcaaaga
    43261 cccggaaatg caggtgcaca ttctctgagt gatttgatcg attgagcctt ccagtccttc
    43321 gggactggaa tttttttgtt cggagaacga agataaggca agtcaatcaa aacaggagaa
    43381 aaacatggct gattgggtaa caggcaaagt cactaaagtg cagaactgga ccgacgccct
    43441 gtttagtctc accgttcacg cccccgtgct tccgtttacc gccgggcaat ttaccaagct
    43501 tggccttgaa atcgacggcg aacgcgtcca gcgcgcctac tcctatgtta actcgcccga
    43561 taatcccgat ctggagtttt acctggtcac cgtccccgat ggcaaattaa gcccacgatt
    43621 ggcggcactg aaaccgggcg atgaagtgca ggtggttagc gaagcagctg gcttctttgt
    43681 tctggatgaa gtaccggact gcgaaacgct atggatgctg gcaaccggta cagcgattgg
    43741 cccttattta tcgattctgc aactaggcaa agatttagat cgcttcaaaa atctggtcct
    43801 ggttcacgcc gcacgttatg ccgccgactt aagctatttg ccactgatgc aagaactgga
    43861 aaaacgctac gaaggcaaac tgcgcattca gacggtggtc agtcgggaaa cggcagcggg
    43921 gtcgctcacc ggacggatac cggcattaat tgaaagtggg gaactggaaa gcgcgattgg
    43981 cctgccgatg aataaagaaa ccagccatgt gatgctgtgc ggcaatccac agatggtgcg
    44041 cgatacacaa cagttgctga aagagacccg gcagatgacg aaacatttac gtcgccgacc
    44101 gggccatatg acagcggagc attactggta agcggttact tatcgataaa cggcacgatg
    44161 agcaaatccg cactcatctt attgatcatc ccgcgatatg ccggcatcaa acggttgata
    44221 aatgagtgat gatgaccaca gacaaggagg tcgcactgct ctttttgcat aatttccagc
    44281 agtgtttccg gcatttctcc gcgttcaata cgcagttttg tcttcggcca ttgaatattt
    44341 ttcgtcagtt tatacagctt gttatccgac ttattcttca acaattgaag aatatcttct
    44401 gttgcaggga agtagatacc cggatacaac tcgcttaagc catcatcaat atgaattaac
    44461 gtcaggtgag cgtcattatg tctggcgagc tccagggctt tattcaccag taaggcatct
    44521 tcttcattcc cggaaattgc cacgccaatg tgtttataag ccatgtttaa ctccttctaa
    44581 agcaactcca tcaagctaac gaacgcagtg ataactcaaa aataatcatc tcggcctggc
    44641 atgagaaagt gaaggccgca tcaagctcaa cttgcccctc cagatcctta atcttatagg
    44701 ccacgctggc cccttcatct gcagaggatg cagcggcggc aagctctttc agtttggtca
    44761 taaaagactc tgcttcttcg cgggtggcaa aaaagcgcga aaacttactg gtacagttgt
    44821 cgttatcaat caccgtaccg atatcaatcg cacatccttt agtactgcat ttatctacga
    44881 catctttcat agggcacctc tgtatattcg cctctctgtc gattacgact taagaataat
    44941 gcgccgttaa gggaagaaca aacaagtaat gatcttatgg attaccattt tttttgatat
    45001 tgcttaaggt gaagagttca gaaggatttt ccgggtattg tcatttgcat cgtggaaata
    45061 acaatcccgg aaaaaaatag cagtaagaga tatattaaag gttaattact tgatttattg
    45121 tcggctttat acttcacatc ctgagtatct ttaccatatt tattttcgcc ttgtgtgcca
    45181 acaaacgcac caagatcgat aagcatcatc accagaatca acgtcgggac aaaacgcccc
    45241 accgcccatt gccagacacc cggtaaaatc gctcagttac ccgccagcag catccacgcc
    45301 acaatcatca gaaatgccca tgcgccggaa cgcccgcgat catgcaagcg cttaacagtt
    45361 actgccgctg ttggccagag caagcacaca aggcaaaacg ccgcggtctg aatatcgagt
    45421 agattcttac ccgccagtga gaaaagcacc agcatgcctg cgaaccacag gcctatccaa
    45481 atccagaaat cacggcgtcc aatacgccct ttaaatgaga ataaccattg ctgtatggtc
    45541 atgtaagttc cttgatggtt gtcttttcca ggattctacc cttttgacaa gggcgacaga
    45601 tatcgtttta atcggagcca gtcataacaa aaggtactgt caatgaagcc agggtgtacg
    45661 ctgttttttc tcttatgttc tgcattaacc gttacaacaa cggcgcatgc acaaacacca
    45721 gatacggcaa cgaccgcgcc ttatctgctg gctggagccc ctactttcga tctctccatc
    45781 agccagtttc gagaagactt taacagccag aatcccagcc tgccactgaa cgaatttcgt
    45841 gccatcgaca gcagtcccga caaagccaat ctcactcgtg ctgcaagtaa aattaacgag
    45901 aacttgtatg cttctacagc gctggagcgc ggtaccttaa aaatcaaaag cattcaaatg
    45961 acctggctac ccatccaggg gccagagcaa aaagcagcga aagcgaaagc tcaggaatac
    46021 atggcagcag tgatccgcac actcacccca ttaatgacca aaatacaaag ccagaaaaaa
    46081 ctgcagtcgc tactaacggc ggggaaaaac aaacgttatt acaccgagac agaaggtgca
    46141 ctgcgttatg ttgtcgcgga caacggcgaa aaggggctga ccttcgctgt tgaaccgatt
    46201 aagctggcgc tatctgaatc gcttgaaggt ttgaataaat gacaaaaagc aaagcctttg
    46261 tgccgatgaa tctctatact gtttcacaga cctgctgccc tgcggggcgg ccatcttcct
    46321 ttattcgctt ataagcgtgg agaattaaaa tgcgacatcc tttagtgatg ggtaactgga
    46381 aactgaacgg cagccgccac atggttcacg agctggtttc taacctgcgt aaagagctgg
    46441 caggtgttgc tggctgtgcg gttgcaatcg caccaccgga aatgtacatc gatatggcga
    46501 agcgcgaagc tgaaggcagc cacatcatgc tgggtgcgca aaacgtggac ctgaacctgt
    46561 ccggcgcatt caccggtgaa acttctgccg ctatgctgaa agacatcggc gctcagtaca
    46621 tcatcatcgg ccactctgaa cgtcgtactt accacaaaga gtctgacgaa ctgattgcga
    46681 aaaaattcgc ggtgctgaaa gagcagggtc tgactccggt tctgtgcatc ggtgaaaccg
    46741 aagctgaaaa cgaagcggga aaaactgaag aagtttgcgc acgtcagatc gacgcggtac
    46801 tgaaaactca gggtgctgcg gcattcgaag gtgcggttat cgcttacgaa cctgtatggg
    46861 caatcggtac tggcaaatct gcaactccgg ctcaggcaca ggctgttcac aaattcatcc
    46921 gtgaccacat cgctaaagtt gacgctaaca tcgctgaaca agtgatcatt cagtacggcg
    46981 gctctgtaaa cgcgtctaac gctgcagaac tgtttgctca gccagacatc gacggcgcgc
    47041 tggttggcgg tgcttctctg aaagctgacg ctttcgcagt gatcgttaaa gctgcagaag
    47101 cggctaaaca ggcttaagtc tgacaggtgc cggatttcat atccggcact tactttcctt
    47161 aactcctcgc cttaacgcaa aatctcacac tcatgatcct ggatttcctc ggctgaagca
    47221 cggttcagcg tcagtagatt tcgttgtgtc gccagcaaca caaacgaatt atcactctgc
    47281 cgtaccatcg ccagcccgta gcttcccata tgttcccgcg cctcaggcac ttcttctgca
    47341 agcatcataa atgggctgcg ttgtaccagt tcgctttccg ttacccgacg cgccaggtat
    47401 tcatgcccgc gcaaaccacc tggcagtggc aaccagcggc tgctgatgtt cgccagattt
    47461 ttatccagtt gttcgcgcac atcaggacga atacaagaga tatgaatatg aaaatggttt
    47521 tgcgtatgcc cggtgcggga gttgatcgcc aaagaaaccg cgcgatcggg aaccggctgg
    47581 ccgtattttt tgctcataaa atcacgcgcc tgccaggcca accaaaagaa gttcggcgtt
    47641 gaaggatcgg tcaacaaagg actttcagta ccgttaatac gatacgttgg catcaacaga
    47701 tattgcagtg gaccattaag atcttttaaa accacgtatc cggcattggg tttgacttcc
    47761 gcacatggcg aaggattttg attttgctgc tgattgggca aacattcctc aaggacaatc
    47821 ttacgtaatg tatccgactc ttcaccggtt aatttccagt aaccaatacc ggcagccaca
    47881 acggcgataa ctatcatcac caaaaaaaga agacccgctt ttttcatctt tttttccctg
    47941 tacctcaaag agatgcaagg gtaacgcaaa atcgtgacaa ataaaaaacc cggtcgcgag
    48001 aaaccaccgg gtttgataat tatcctaggg aaatcagcgt ttgctgatct gatcgaacgt
    48061 accgccgtta gcaaaatgct ctttttgcgc tttcgtccag ccgccgaact cttcatcaat
    48121 ggtgaataac ttcagctttg gaaacgcatt ttcgtacttt ttcgctactt cagcgtcgcg
    48181 cggacggtag tagtttttcg cggcaatttc ctgaccttct ggcgagtaga gatatttcag
    48241 gtaggcttcc gccacctctt tggtgccttt tttctcgacc actttatcga ccaccgacac
    48301 cgttggctct gcgaggatag actcactcgg cgtgacgatt tcgaatttat ctttccccag
    48361 ttcattcgct gccagcagag cttcgttttc ccaggcgatc agtacatcgc caatcccgcg
    48421 ctcgacaaag gtgttagtgg agccgcgcgc gccagaatcc agaacttcga cgtttttata
    48481 cagtgcccga acaaaatcct gtgcttttgc ctgatcgttg ttgttgtgat gcagcgcgta
    48541 gccccaggca gccaggtagt tccagcgtgc gccaccagag cttttcggat taggcgtgat
    48601 caccgaaaca cccggtttaa tcagatcgtt ccagtcatgg atctgcttcg gattgccctt
    48661 acgcaccagg aaaacaatgg tggaagtgta cggtgcggag ttatccggca gacgtttgat
    48721 ccactcttta tcaatccgcc cgcgttccgc aatcgcgtcc acgtcatagg ccagagccag
    48781 cgtgacaaca tcagcttcaa taccgttgat taccgacgtc gcttgtttac ctgaaccacc
    48841 gtgtgactga cgaatcacca cgttatcacc agtttgctgt ttccagtggg cgctgaatgc
    48901 cttgttgtac tgttcgtaca attcgcgcgt tggatcatat gaaacgttaa gaagctgaat
    48961 atcctttgcc ataacgctgg ttgccgccag caaaaatgtt aaccctacgc cccacttgtt
    49021 catcgcccga ctctcttatg ttgtgttgtg atgagcaaag cgtgccagaa ggttaaccaa
    49081 acattaaaga ataaaaaaag attggctata acttgcgggt atatgttgag ggattaaaaa
    49141 ggcggaagaa atccgcctca tattgctgac aaagtgcgct ttgtccatgc cggatgcggc
    49201 gcgaacgcct tatccggcct acaaaagttt gcaaattcaa taaattgcag aattcatgta
    49261 ggcctgataa gcgaagcgca tcaggcattt ttgcttctgt catcggtttc aggctaaagg
    49321 aatctgcctt tttccgaaat cattaataca gttttttcgc gcagtccagc cagtcacctt
    49381 tgaacggacg cttcatgttt tcgatagcgt cgatgatgtc gtggtgaacc agctgttcgt
    49441 tctggatacc tacacaacga ccgccgtaac ctgccagcag cagatcgata gcgtaagcgc
    49501 ccatacggga agccagaata cggtcgtaag gcaccggaga accaccgcgc tggatgtggc
    49561 ccagcacagt tgcgcgggtt tcacgaccgg tttctttctc gatgaaatgc gccagttcgt
    49621 caacatcaca catatgttcg gtaatcgcca cgatcgcgtg ttttttacct ttcgcgatac
    49681 ccgctttgat ttcgtttacc aggtcttcac ggctgaattc aacttccgga actacaacga
    49741 attcacagcc accggcaatg gccgcagcca gggtcagatc gccacaataa cggcccatca
    49801 cttccaccac ggaaatacgc tggtgagaag aagaggtgtc acgcagacgg tcgatcgctt
    49861 ctacaacggt gctcagcgca gtgaagaaac cgatagtgta gtcagtgcct ttgatgtcgt
    49921 tgtcgatagt gcccggcaga ccgatgcacg ggaagcccat ttcggtcaga cgcattgcac
    49981 ccatgtagga accgtcaccg ccgataacca ccagcgcgtc gataccacgt tttttcaggt
    50041 tttcgatagc cacggcgcgg atgttctcgt cgcggaattc cgggaaacgc gcagaaccga
    50101 ggaacgtacc gccacggttg atcatgtcag acacgctgta acggtctagc tgtaccatac
    50161 ggtcttcata cagacccaga tagccgtcat aaatacccat tacttccaga ccttctgtca
    50221 gcgcagaacg aacaaccccg cgaattgcgg cgttcatgcc tggcgcatca ccgccgcttg
    50281 tcaacacacc tattttctta atcatgacta cctctgaact ttggaatgca aaatgaaatc
    50341 tgttgccgga agtcttcttg cacatcgaag tgatccaacg aatgtgcaaa tagtataaca
    50401 atcacttcct gctgaattga ttcaggtcag gccaaatggc ggtattttat acacaaaatg
    50461 cgggtctggc tctcttttat actgattatg aaagcataga ccgtttaccc tccctgggta
    50521 cgacggaaca ggggtcctga tggataatca catccgatcc cggaaaacgc cgtaaaatag
    50581 cctgctctac ctgatccgcc accatatgtg cctgaaccaa aggcagagag tcttccattt
    50641 ccaaatgaat ctgaataaag cgggtcggcc ctgactgccg cgtgcgaaga tcgtgagcgc
    50701 cgctaacacc cggccaggaa gtcacgatat caataatttc ttgccgttct tcatcaggca
    50761 atgcgcgatc cagtaatgac tgtaccgcct catatcccat gcgtaacgcg ctatataaaa
    50821 tatagatgcc gattcccaat gcaaacagag catcggcgcg atgccagccg taccaagaca
    50881 accccagcgc cagcagaatt gcgccgttca tcataacatc agactggtaa tgtagcatat
    50941 cagcccgcac cgcctggctt tgcgtccggc gcaccaccca ccgctgaaac gagacaagga
    51001 taatcgtaca aattagcgcc acaattgtca cgataacccc gacgcctgga tctgtcatcg
    51061 gtgttggaga taccagatgt tgaatacccg tcaaaaacag gaatagtgcc gaaccggaga
    51121 taaacatact ttgcgccagc gccgcgaggg actcagcttt accgtgacca aacgagtgat
    51181 tatcgtcggc aggttgcagg gaatatcgca ccaccagtaa attcgtcaac gacgcgccga
    51241 tatccaccag cgaatccacc agcgcggcga gaatactcac cgacccggta taccaccatg
    51301 caaaaatttt aatcagcaat agcaacgaag ccatcgccgt cgcagcaatc gccgcccgac
    51361 tgaccagccg tccataagat tgattcataa atactcccgc tatcaactga cgctagtata
    51421 acggaagcaa atcatctgca atgcattaag cagcaggcaa attgaggata aaaaaaaccc
    51481 cacatcatgt gggggaagac agggatggtg tctatggcaa ggaaaacagg gtttactact
    51541 gggaacgtga gttgctacta ctcaatagct tcaacgatga acttttttgc cattgcgtca
    51601 cgtcacgcaa ctgctccatt cgttgttgat gtttctcgtt taaaaccgct tgctgctccg
    51661 gcgttaacag gcgatacatt tggttgcgga cttttgccat ctcaacctga cgagcaattt
    51721 gctcattcgc cattttttct gcctgtgcgc gcacagcgtt ttcatcaaaa ttttctgcgg
    51781 tgacaaggcg atgcattgtc tccagttcgc taacattaac aggaggctgt tcgtgccggg
    51841 cctgttgcat aagatctcgc atctgctgac gctgatgttc ggttaaactt atgccgtcga
    51901 acatatggct ctgcgtactg cgctgcgtaa gttcttcacc cggatgccag ttatcgcctg
    51961 aaccgacttc agcagcgtgg cttaatgaac tgactgccag cgttgaggcc atgacggcag
    52021 cggtaactat gcgcatcatt tgctcccaaa atctttctgt cgcgattcaa cgatagagag
    52081 tttacgattc aggctgcaaa catgcgtcag ggggtgtaaa acaacgtaaa gtcatggatt
    52141 agcgacgtct gatgacgtaa tttctgcctc ggaggtattt aaacaatgaa taaaatcctg
    52201 ttagttgatg atgaccgaga gctgacttcc ctattaaagg agctgctcga gatggaaggc
    52261 ttcaacgtga ttgttgccca cgatggggaa caggcgcttg atcttctgga cgacagcatt
    52321 gatttacttt tgcttgacgt aatgatgccg aagaaaaatg gtatcgacac attaaaagca
    52381 cttcgccaga cacaccagac gcctgtcatt atgttgacgg cgcgcggcag tgaacttgat
    52441 cgcgttctcg gccttgagct gggcgcagat gactatctcc cgaaaccgtt taatgatcgt
    52501 gagctggtgg cacgtattcg cgcaatcctg cgccgttcgc actggagcga gcaacagcaa
    52561 aacaacgaca acggttcacc gacactggaa gttgatgcct tagtgctgaa tccaggccgt
    52621 caggaagcca gcttcgacgg gcaaacgctg gagttaaccg gtactgagtt taccctgctc
    52681 tatttgctgg cacagcatct gggtcaggtg gtttcccgtg aacatttaag ccaggaagtg
    52741 ttgggcaaac gcctgacgcc tttcgaccgc gctattgata tgcacatttc caacctgcgt
    52801 cgtaaactgc cggatcgtaa agatggtcac ccgtggttta aaaccttgcg tggtcgcggc
    52861 tatctgatgg tttctgcttc atgataggca gcttaaccgc gcgcatcttc gccatcttct
    52921 ggctgacgct ggtgctggtg ttgatgttgg ttttgatgtt acccaagctc gattcacgcc
    52981 agatgaccga gcttctggat agcgaacagc gtcaggggct gatgattgag cagcatgtcg
    53041 aagcggagct ggcgaacgat ccgcccaacg atttaatgtg gtggcggcgt ctgttccggg
    53101 cgattgataa gtgggcaccg ccaggacagc gtttgttatt ggtgaccacc gaaggccgcg
    53161 tgatcggcgc tgaacgcagc gaaatgcaga tcattcgtaa ctttattggt caggccgata
    53221 acgccgatca tccgcagaag aaaaagtatg gccgcgtgga actggtcggt ccgttctccg
    53281 tgcgtgatgg cgaagataat taccaacttt atctgattcg tccggccagt agttctcaat
    53341 ccgatttcat taacttactg tttgaccgcc cgctattact gctgattgtc accatgttgg
    53401 tcagtacgcc gctgctgttg tggttggcct ggagtctggc aaaaccggcg cgtaagctga
    53461 aaaacgctgc cgatgaagtt gcccagggaa acttacgcca gcacccggaa ctggaagcgg
    53521 ggccacagga attccttgcc gcaggtgcca gttttaacca gatggtcacc gcgctggagc
    53581 gcatgatgac ctctcagcag cgtctgcttt ctgatatctc tcacgagctg cgcaccccgc
    53641 tgacgcgtct gcaactgggt acggcgttac tgcgccgtcg tagcggtgaa agcaaggaac
    53701 tcgagcgtat tgaaaccgaa gcgcaacgtc tggacagcat gatcaacgat ctgttggtga
    53761 tgtcacgtaa tcagcaaaaa aacgcgctgg ttagcgaaac catcaaagcc aaccagttgt
    53821 ggagtgaagt gctggataac gcggcgttcg aagccgagca aatgggcaag tcgttgacag
    53881 ttaacttccc gcctgggccg tggccgctgt acggcaatcc gaacgccctg gaaagtgcgc
    53941 tggaaaacat tgttcgtaat gctctgcgtt attcccatac gaagattgaa gtgggctttg
    54001 cggtagataa agacggtatc accattacgg tggacgacga tggtcctggc gttagcccgg
    54061 aagatcgcga acagattttc cgtccgttct atcggaccga tgaagcacgc gatcgtgaat
    54121 ctggcggtac aggattgggg ctggcgattg ttgaaaccgc cattcagcag catcgtggct
    54181 gggtgaaggc agaagacagc ccgctgggcg gtttacggct ggtgatttgg ttgccgctgt
    54241 ataagcggag ttaaactccg catttgtagg caggataagg cgtttacgcc gcatccggca
    54301 tttgagcagg atgcctgatg cgacgctgat agcgtcttat caggcctaca ctccgggggt
    54361 tattttcccc acaaccggcg ggaaaaatct tcgatcttgc cgctcagtcg gcgcttttgc
    54421 atcgtccgcg tccagctttt cgataacccg gcggcggaga gtaaacggtg atactgatcg
    54481 tcatcaaacg gcatatgcca tgcgatggcg gcagcctcct gcacggtcac atcgctgaca
    54541 cgggaaacca actccagcgg cgcgtccgca gacactttcc ctggcgctat cacgctatac
    54601 aaccacccca ccttacctgt gttttgcatc aactgcgcta tatcgctgat atcaaaatgg
    54661 taattgagtt tatagcaagg cgagcgcggc tggctgacct gaattaatgc ctctccccag
    54721 cggaaaatat cgcccatatg aacattactt tccgtgagac cgtcggttga gagattttcg
    54781 ccaaacgcag gcgcaacaaa caactccgcc tgttccggaa attcccgcac ccagtagaga
    54841 tagtgctcac gaggataatg acacagcgct ctgtctggcc cgccgtgaac ctttttctcc
    54901 gcctgctcgt caccttccag ccccagctcc gtcagcatca actcaccatc aacctggatt
    54961 ttggcaattg cgctgggttt gccttcggga taagcctgaa tcttgcctgt gtatacatca
    55021 accggatatc gcatctgttc cctctccacg agaaatttcc ccattagacc atatttattt
    55081 tatgtcagcc gtgctgtttt cgcggtaagt atccaggcag gaactcaata gcaaaccgga
    55141 cgaacgttag ttcatccggc tgtgggg
//
LOCUS       DAJTRD010000018        53934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTRD010000018 DAJTRD010000000
VERSION     DAJTRD010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.018"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            96..395
                     /locus_tag="OO315_001172"
     CDS             96..395
                     /locus_tag="OO315_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5890164.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            405..929
                     /gene="ygaP"
                     /locus_tag="OO315_001173"
     CDS             405..929
                     /gene="ygaP"
                     /locus_tag="OO315_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5890165.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(976..1380)
                     /gene="stpA"
                     /locus_tag="OO315_001174"
     CDS             complement(976..1380)
                     /gene="stpA"
                     /locus_tag="OO315_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5890166.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2048..2497
                     /gene="alaE"
                     /locus_tag="OO315_001175"
     CDS             2048..2497
                     /gene="alaE"
                     /locus_tag="OO315_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5890167.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2534..2878)
                     /locus_tag="OO315_001176"
     CDS             complement(2534..2878)
                     /locus_tag="OO315_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5890168.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3030..3260
                     /locus_tag="OO315_001177"
     CDS             3030..3260
                     /locus_tag="OO315_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890169.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3607..3852
                     /gene="nrdH"
                     /locus_tag="OO315_001178"
     CDS             3607..3852
                     /gene="nrdH"
                     /locus_tag="OO315_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5890170.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3849..4259
                     /gene="nrdI"
                     /locus_tag="OO315_001179"
     CDS             3849..4259
                     /gene="nrdI"
                     /locus_tag="OO315_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5890171.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4232..6376
                     /gene="nrdE"
                     /locus_tag="OO315_001180"
     CDS             4232..6376
                     /gene="nrdE"
                     /locus_tag="OO315_001180"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5890172.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6386..7345
                     /gene="nrdF"
                     /locus_tag="OO315_001181"
     CDS             6386..7345
                     /gene="nrdF"
                     /locus_tag="OO315_001181"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5890173.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7700..8902
                     /gene="proV"
                     /locus_tag="OO315_001182"
     CDS             7700..8902
                     /gene="proV"
                     /locus_tag="OO315_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5890174.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8895..9959
                     /gene="proW"
                     /locus_tag="OO315_001183"
     CDS             8895..9959
                     /gene="proW"
                     /locus_tag="OO315_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5890175.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10017..11009
                     /gene="proX"
                     /locus_tag="OO315_001184"
     CDS             10017..11009
                     /gene="proX"
                     /locus_tag="OO315_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5890176.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11201..12380
                     /locus_tag="OO315_001185"
                     /pseudo
     CDS             11201..12380
                     /locus_tag="OO315_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12504..13241
                     /gene="ygaZ"
                     /locus_tag="OO315_001186"
     CDS             12504..13241
                     /gene="ygaZ"
                     /locus_tag="OO315_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5890177.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13231..13566
                     /gene="ygaH"
                     /locus_tag="OO315_001187"
     CDS             13231..13566
                     /gene="ygaH"
                     /locus_tag="OO315_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5890178.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13657..14187
                     /gene="emrR"
                     /locus_tag="OO315_001188"
     CDS             13657..14187
                     /gene="emrR"
                     /locus_tag="OO315_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5890179.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14314..15486
                     /gene="emrA"
                     /locus_tag="OO315_001189"
                     /pseudo
     CDS             14314..15486
                     /gene="emrA"
                     /locus_tag="OO315_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15503..17041
                     /gene="emrB"
                     /locus_tag="OO315_001190"
     CDS             15503..17041
                     /gene="emrB"
                     /locus_tag="OO315_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5890180.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="OO315_001191"
     CDS             complement(17105..17620)
                     /gene="luxS"
                     /locus_tag="OO315_001191"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5890181.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="OO315_001192"
     CDS             complement(17770..19326)
                     /gene="gshA"
                     /locus_tag="OO315_001192"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5890182.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19399..19827)
                     /locus_tag="OO315_001193"
     CDS             complement(19399..19827)
                     /locus_tag="OO315_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5890183.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="OO315_001194"
     CDS             complement(19824..20390)
                     /gene="yqaB"
                     /locus_tag="OO315_001194"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5890184.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20538..20614)
                     /locus_tag="OO315_001195"
     tRNA            complement(20538..20614)
                     /locus_tag="OO315_001195"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20578..20580),aa:Arg,seq:acg)
     gene            complement(20679..20755)
                     /locus_tag="OO315_001196"
     tRNA            complement(20679..20755)
                     /locus_tag="OO315_001196"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20719..20721),aa:Arg,seq:acg)
     gene            complement(20759..20851)
                     /locus_tag="OO315_001197"
     tRNA            complement(20759..20851)
                     /locus_tag="OO315_001197"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20815..20817),aa:Ser,seq:gct)
     gene            complement(21167..21352)
                     /gene="csrA"
                     /locus_tag="OO315_001198"
     CDS             complement(21167..21352)
                     /gene="csrA"
                     /locus_tag="OO315_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5890185.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(21587..24217)
                     /gene="alaS"
                     /locus_tag="OO315_001199"
     CDS             complement(21587..24217)
                     /gene="alaS"
                     /locus_tag="OO315_001199"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5890186.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(24345..24845)
                     /gene="recX"
                     /locus_tag="OO315_001200"
     CDS             complement(24345..24845)
                     /gene="recX"
                     /locus_tag="OO315_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5890187.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(25088..26149)
                     /gene="recA"
                     /locus_tag="OO315_001201"
     CDS             complement(25088..26149)
                     /gene="recA"
                     /locus_tag="OO315_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5890188.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(26229..26726)
                     /gene="pncC"
                     /locus_tag="OO315_001202"
     CDS             complement(26229..26726)
                     /gene="pncC"
                     /locus_tag="OO315_001202"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5890189.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(26871..27956)
                     /gene="mltB"
                     /locus_tag="OO315_001203"
     CDS             complement(26871..27956)
                     /gene="mltB"
                     /locus_tag="OO315_001203"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5890190.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            28212..28774
                     /gene="srlA"
                     /locus_tag="OO315_001204"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             28212..28774
                     /gene="srlA"
                     /locus_tag="OO315_001204"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            28771..29730
                     /gene="srlE"
                     /locus_tag="OO315_001205"
                     /gene_synonym="gutE"
     CDS             28771..29730
                     /gene="srlE"
                     /locus_tag="OO315_001205"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5890191.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            29741..30112
                     /gene="srlB"
                     /locus_tag="OO315_001206"
                     /gene_synonym="gutB"
     CDS             29741..30112
                     /gene="srlB"
                     /locus_tag="OO315_001206"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5890192.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            30116..30895
                     /gene="srlD"
                     /locus_tag="OO315_001207"
     CDS             30116..30895
                     /gene="srlD"
                     /locus_tag="OO315_001207"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5890193.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            31000..31359
                     /gene="gutM"
                     /locus_tag="OO315_001208"
     CDS             31000..31359
                     /gene="gutM"
                     /locus_tag="OO315_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5890194.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            31426..32199
                     /gene="srlR"
                     /locus_tag="OO315_001209"
                     /gene_synonym="gutR"
     CDS             31426..32199
                     /gene="srlR"
                     /locus_tag="OO315_001209"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5890195.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            32192..33157
                     /gene="gutQ"
                     /locus_tag="OO315_001210"
     CDS             32192..33157
                     /gene="gutQ"
                     /locus_tag="OO315_001210"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5890196.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(33154..34668)
                     /gene="norR"
                     /locus_tag="OO315_001211"
     CDS             complement(33154..34668)
                     /gene="norR"
                     /locus_tag="OO315_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5890197.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            34855..36294
                     /gene="norV"
                     /locus_tag="OO315_001212"
     CDS             34855..36294
                     /gene="norV"
                     /locus_tag="OO315_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5890198.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            36291..37424
                     /gene="norW"
                     /locus_tag="OO315_001213"
     CDS             36291..37424
                     /gene="norW"
                     /locus_tag="OO315_001213"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5890199.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(37552..39804)
                     /gene="hypF"
                     /locus_tag="OO315_001214"
     CDS             complement(37552..39804)
                     /gene="hypF"
                     /locus_tag="OO315_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5890200.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(39957..40484)
                     /gene="hydN"
                     /locus_tag="OO315_001215"
     CDS             complement(39957..40484)
                     /gene="hydN"
                     /locus_tag="OO315_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5890201.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(40633..41643)
                     /gene="ascG"
                     /locus_tag="OO315_001216"
     CDS             complement(40633..41643)
                     /gene="ascG"
                     /locus_tag="OO315_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5890202.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(41657..41776)
                     /locus_tag="OO315_001217"
     CDS             complement(41657..41776)
                     /locus_tag="OO315_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5890203.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(41786..43495)
                     /gene="hycE"
                     /locus_tag="OO315_001218"
     CDS             complement(41786..43495)
                     /gene="hycE"
                     /locus_tag="OO315_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5890204.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(43513..44436)
                     /gene="hycD"
                     /locus_tag="OO315_001219"
     CDS             complement(43513..44436)
                     /gene="hycD"
                     /locus_tag="OO315_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5890205.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(44439..46286)
                     /gene="hycC"
                     /locus_tag="OO315_001220"
     CDS             complement(44439..46286)
                     /gene="hycC"
                     /locus_tag="OO315_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5890206.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLY
                     HLLNHSLFKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPL
                     NGFAGEWVIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAP
                     RTKEAENATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQP
                     MITLLLIACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQA
                     FAPVLKLRKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(46323..46934)
                     /gene="hycB"
                     /locus_tag="OO315_001221"
     CDS             complement(46323..46934)
                     /gene="hycB"
                     /locus_tag="OO315_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5890207.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(47059..47520)
                     /gene="hycA"
                     /locus_tag="OO315_001222"
     CDS             complement(47059..47520)
                     /gene="hycA"
                     /locus_tag="OO315_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5890208.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            47732..48082
                     /gene="hypA"
                     /locus_tag="OO315_001223"
     CDS             47732..48082
                     /gene="hypA"
                     /locus_tag="OO315_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5890209.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            48086..48958
                     /gene="hypB"
                     /locus_tag="OO315_001224"
     CDS             48086..48958
                     /gene="hypB"
                     /locus_tag="OO315_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5890210.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            48949..49221
                     /gene="hypC"
                     /locus_tag="OO315_001225"
     CDS             48949..49221
                     /gene="hypC"
                     /locus_tag="OO315_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5890211.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            49221..50342
                     /gene="hypD"
                     /locus_tag="OO315_001226"
     CDS             49221..50342
                     /gene="hypD"
                     /locus_tag="OO315_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5890212.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            50339..51349
                     /gene="hypE"
                     /locus_tag="OO315_001227"
     CDS             50339..51349
                     /gene="hypE"
                     /locus_tag="OO315_001227"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5890213.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            51423..53501
                     /gene="flhA"
                     /locus_tag="OO315_001228"
     CDS             51423..53501
                     /gene="flhA"
                     /locus_tag="OO315_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5890214.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(53538..53891)
                     /locus_tag="OO315_001229"
     CDS             complement(53538..53891)
                     /locus_tag="OO315_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5890215.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT        12826 a        14570 c        13815 g        12723 t
ORIGIN      
        1 agcttacgct tttactctca tttacgctgc tgaggctggt atatgttgca tttttagttt
       61 agagtttact taatttagaa aatacttaaa taaatatgac tgaactcgcg caattacagg
      121 ccagtaccga acaggcagcg gccttattga aagcaatgag ccaccctaaa cggttgctga
      181 ttctgtgcat gcttagcggt tcccccggca ccagcgcggg agagctgacg cgcattaccg
      241 gactgagcgc ctctgcgaca tcacagcatc tcgctcgtat gcgggacgaa gggcttatca
      301 acagccaacg ggatgcccaa cgcattctat attccattaa aaatgaggcg gtaaatgcca
      361 ttatcgccac cctgaaaaat gtctattgtc cgtaaggagt cactatggct ttgacaacca
      421 tttcgccgca tgatgcacaa gaattaatcg cacgcggcgc aaagttaatc gatattcgtg
      481 acgctgatga atatcttcgt gaacatattc ctgaagcaga tctggctcca ttatccgtgc
      541 tggaacagtc aggtcttcca cctaaattac gtcgcgagca aattatattc cactgccagg
      601 caggtaaacg caccagtaat aacgccgata aattagcagc gattgccgcc cccgcagaaa
      661 tatttttact cgaagatggg attgatggct ggaaaagagc tggattgcca gtagcggtaa
      721 ataaatctca acccttgccg ttaatgcgcc aggtgcagat cgctgcgggt ggtttaatat
      781 taaacggcgt tgtactgggt tataccgtaa atagcggttt cttcttatta agtggctttg
      841 ttggtgccgg gttactgttt gcaggaatca gcggtttttg tggaatggca aggttgttag
      901 ataagatgcc gtggaaccaa cgagcttgag aagcgacgcc ggacgcgccc tagcagcgac
      961 atccggcctc agtaattaga tcaggaaatc gtcgagagat ttaccttctg ccagcgcctg
     1021 agcaattggc ttcggtgtac ggccctgacc ggtccaggtt ttagtttcac cgttaacatc
     1081 agtgaattta tatttcgccg gacgcggctg gcgtttttta ccagcacgtg gtgcagcagc
     1141 agagctatta cccaataact cttccgggtt aattccgtca gctttcatca gctccagcca
     1201 ggtgctaatt ttttcctgac gctcagccag ttcacgctgc tgctgttctt cttcttcacg
     1261 tctttcttta gtgacaaccc tgaatttttc gagcatttct tcaagaacgt caatggagaa
     1321 ttcgcgagcc atcgcacgga gggtgcgaat gttatttaaa ctttgtaaca ttacggacat
     1381 aataaagaaa accttttaac gccaaaacaa aaaagtattt cgtcgcgtag tgtaagcgat
     1441 cacttattat atttccacaa aatttaatct atttacagtc ctgcgcgaga ttatttcagc
     1501 tgttattttc tgaaactctc agaataattt caagattata tattagccat tttgatgagt
     1561 aatttaggtt attggtgaac agagtggcgc tctctgctcg ctaactccgc aagcctgaca
     1621 aaaccgaagt gaataaaaat acaatataac agaataaaag aaaacacccc tcactggtgc
     1681 tttctgctgt taatgtacat tttctggcat caaaaatgct gtaaatgacc cggtcatgac
     1741 aaacaccgaa ttctctacgc ttatagcgtt acctcccccc caaactttca ttagtgaaat
     1801 taaaggcaag atcatatcta attgatttaa ttgaaaataa atcatattgg catgtaaaaa
     1861 tccacaatgt acaaaaaacg agccgttacg gaatatttta tctacaaaaa ctaactaaat
     1921 aaaaattttt cactaattga ttagtcatag gcagcgatat acgctatgca aaaatgcaga
     1981 tggcaatgag atccactgct ttcatctcca ttaacatccc attacgcttt tattaaggag
     2041 cattagcatg ttctcaccgc agtcacgctt gcgtcatgca gttgcagata cgttcgcgat
     2101 ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt ttcctctccg gaatgagctt
     2161 cgaacagtct ttttattcca gattggtagc gattccggtg aacatcttaa ttgcatggcc
     2221 atacggtatg taccgcgatc tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg
     2281 gataaaaaat ctggctgata tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc
     2341 gatcttgtta gtggtgggcg cagactggca tcagattatg gcggcggtca gttcaaacat
     2401 cgttgtttcg atgttgatgg gggcggttta tggctacttc ctcgattatt gccgccgact
     2461 gtttaaagtc agccgttacc agcaggtaaa agcctgactc ttcttttcgc gactggcatc
     2521 gccagtcgcg tctttaactg gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa
     2581 cacgcgaact aaagccatgc gggataccgt aatgctcgtg tcgctcgcct gctaaataga
     2641 gcggaaattg ctggtaatga tcgcaagttt taatatcgga agcgggttca gccagtgttg
     2701 aactgcgttc ttttaatgtt ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc
     2761 gattaacata gacataattt tccccacggc ggtaaccata cgctttctgg gttaccacat
     2821 cgacagtaaa accgactttt tcaagtacgc gcgccacctc gtctggtcgt aaatacatat
     2881 ttgatcctcg ttattttcaa ccgcgggcct accttacctg attgcgcatt cacaacgctt
     2941 tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga aaattatggt ctacactgaa
     3001 aattacatcg aattctaatg ggggatcata tgtttaaccg accgaaccgc aacgacgttg
     3061 atgatggcgt gcaggatatc cagaatgatg tcaatcaatt agctgacagc ctcgaatctg
     3121 tattgaagtc ctggggcagc gacgccaaag gggaagctga agccgcacgc agtaaagctc
     3181 aggcgttgct gaaagaaacc agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg
     3241 cgcgcgatgc cgttggctga gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg
     3301 gtacagcagc tgcggtaggt atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt
     3361 gcaaaaatga taataaatac gcgtctttga ccccgaagcc tgtcttcggg gtttcttttt
     3421 gcctggtgaa tcacaaaaat ccccctcccc cgtcacgctc atatccaggg taatttcgat
     3481 cactatttgc tatatattgt gtagttgaat cttttttcaa ctacatctag tatctctgta
     3541 tcaacagaga gacaacccga cgcgtatcat cgcgccgtat cttcatttta aacggaaata
     3601 cgaatcatgc gcataactat ttatactcgt aacgattgcg ttcagtgcca cgccaccaaa
     3661 cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa
     3721 gcagcggaag cgttgcgtgc tcagggcttt cgtcagttgc cggtagtgat tgctggcgat
     3781 cttagctggt ctggtttccg tccggacatg attaaccgtc tgcatccagc gccacacgcg
     3841 gccagtgcat gagccagctc gtctacttct ccagcagctc cgaaaacacg cagcgtttta
     3901 tcgaacgttt aggtctgccc gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg
     3961 tagacgagcc ttacatcctg atcgtgccct cttacggcgg cggcggtacg gctggcgcgg
     4021 tgccacgaca ggtaattcgc tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg
     4081 ttattgcttc tggtaatcgc aactttggtg aggcgtatgg ccgcgccgga gatgtgattg
     4141 cccggaaatg cggcgtgccg tggctgtacc gttttgaact catgggtacg caaagcgata
     4201 tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg acaaccgcag aatgcctgac
     4261 gcaggaaacg atggattacc acgcgctgaa tgcgatgctt aatctttacg atagcgcagg
     4321 tcgcattcag ttcgataaag accgccaggc cgttgacgcc tttattgcga cgcatgtgcg
     4381 tccgaacagt gtgaccttca gtagccagca gcagcgcctg aactggctgg tcaacgaagg
     4441 ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc
     4501 ccacgcacac accagcggtt ttcgtttcca gacattcctc ggggcatgga agttttacac
     4561 cagctatacg ttgaagacat tcgacggtaa acgttatctg gaagattttg ccgatcgagt
     4621 cacgatggtg gcgctgacgc tggcacaagg cgatgagacg ctggcgttgc aactgaccga
     4681 tgaaatgctg tcaggacgct ttcagccagc cacgccaaca ttcctcaact gcggtaagca
     4741 gcagcgcggc gaactggttt cctgtttttt gctgcgtatt gaagacaata tggagtcgat
     4801 tggtcgggcg gtaaattccg cactgcagct gtcgaaacgc ggcggcggcg tagcattttt
     4861 gctgtcgaat ctgcgggaag cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg
     4921 cgtaattccg gtgatgaaaa tgttggaaga cgcattttcc tatgccaacc aactcggcgc
     4981 gcgtcagggg gcaggtgcag tctatttaca tgctcatcat cccgatattc tgcgttttct
     5041 cgacacgaaa cgggaaaatg ccgacgaaaa aatccgcatt aaaacactgt cgctgggcgt
     5101 ggtgatcccg gatatcactt tccatctggc aaaagagaat gcgcagatgg cgctgttttc
     5161 gccctatgac gttgagcgag tttatggcaa gccgtttgcc gatatcgcca ttagcgaaca
     5221 ctatgacgaa ctggttgccg atgaacgcat ccgcaaaaaa tacctcaacg cccgtgattt
     5281 cttccagcga ctggcagaaa tccagtttga gtccggctat ccctacatca tgtatgaaga
     5341 cacagtaaac cgtgctaacc ctatcgccgg gcgcataaat atgagtaatc tctgctcaga
     5401 aattttgcag gttaacagcg cctcagagta tgacgagaat ctcgactatg cccgcacagg
     5461 ccatgatatt tcctgcaatt taggttcgtt gaatattgcg cacaccatgg attcccccga
     5521 tttttcccgc acggtagaga ctgccgtgcg cggtttaacg gcggtgtcag atatgagtca
     5581 tatccgcagc gtgccgtcca tcgaagccgg aaatgccgcc tcgcacgcca tcggactggg
     5641 gcagatgaat ttacatggtt atctggcgag ggaaggcatc gcttatggtt cgccggaagc
     5701 cctggatttc accaatctct atttctatac catcacctgg cacgcactgc gtacctcgat
     5761 gttgctggcg cgcgaacgcg gtgaaacctt cgccgggttc aaacagtcac gctatgccag
     5821 tggtgaatat ttcagccaat atctgcaagg taactggcag ccgaaaacgg cgaaagttgg
     5881 cgaactgttt gcccgcagct gtattacgtt acctacccgt gagatgtggg cgcaactgcg
     5941 cgacgacgtg atgcgctacg gcatatacaa ccagaatctc caggcggtgc cgccaaccgg
     6001 gtctatctct tatatcaacc atgctacgtc gagtattcat ccgattgtgg cgaaagtaga
     6061 gatacgcaaa gagggcaaaa caggacgcgt ttactaccct gccccgttta tgactaacga
     6121 aaatctggcg ctgtatcagg acgcttacga aattggcgca gaaaagatca tcgacaccta
     6181 cgcggaagcg actcgccatg tcgatcaggg gctgtcgctg acgctgtttt tccccgatac
     6241 cgccaccact cgcgatatca acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac
     6301 gctctattac atccgcctgc gtcagatggc gctggaaggc actgaaattg aaggctgcgt
     6361 ctcctgtgca ctttaaggaa tatctatgaa actctcacgt atcagcgcca tcaactggaa
     6421 caagatatct gacgataaag atctggaggt gtggaatcgc ctgaccagca atttctggct
     6481 accagaaaag gtgccgctgt cgaacgatat tcccgcctgg cagacattaa ctgtcgtaga
     6541 acaacaactg acgatgcgcg tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt
     6601 tatcggcgcg ccttcgctga tgcccgatgc gctcacgcct catgaagagg cggtattatc
     6661 gaatatcagc tttatggaag cggttcatgc ccgctcttac agttcgattt tctcgacgct
     6721 gtgccagacc aaagatgtcg atgccgccta cgcctggagt gaagaaaacg caccgttgca
     6781 gcgaaaagct cagattattc agcaacatta tcgcggtgat gatccgctga aaaagaaaat
     6841 cgccagtgtg tttcttgaat cttttttgtt ctattccggt ttctggctgc cgatgtattt
     6901 ttccagccgc ggaaagctga ccaataccgc ggacctgatc cgtctgatta tccgcgatga
     6961 agcagtccac ggttactaca taggctataa atatcagaaa aacatggaaa agatatctct
     7021 gggacaacgt gaagagttga agagtttcgc cttcgatttg ttgctggaac tctacgacaa
     7081 cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc
     7141 gtttctctgt tacaacacca ataaggcttt gatgaatctg ggctacgaac cgttatttcc
     7201 cgcagaaatg gcggaagtga atccggcaat cctcgccgcg ctttcgccga atgccgatga
     7261 aaatcacgat ttcttttccg gttcaggctc ctcttatgtg atggggaaag cggttgaaac
     7321 agaagatgaa gactggaatt tctgagggtg ttattttcaa aaatatcact acccgcagca
     7381 gggaaataat tcccgccaaa tagcttttta tcacgcaaat attctgtagt gatctacact
     7441 gatactctgt tgcattattc gcctgaaact acaatattca ggcgtttttt cgctatctat
     7501 gacaaaaaat atcaactttt ctcgatttgc tctcagccct tatatcacgg aaaattccgg
     7561 cgatttgctc gcatcaatat tcatgccaca tttgccctca ggggttgcct cagattctca
     7621 gtatgttagg gtagaaaaaa gtgactattc ccattgggta atatatcgac ataaacaaat
     7681 aaagaaatca ttctattgca tggccattaa attaaaaatt aaaaatcttt ataaaatatt
     7741 tggtgagcat ccacagcgag cgttcaaata tatcgaacaa ggactttcaa aagaacaaat
     7801 tctggaaaaa actgggctat cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg
     7861 cgagatattt gtcatcatgg gattatccgg ctcgggtaaa tccacaatgg tacgccttct
     7921 caatcgcctg attgaaccca cccgcgggca agtgctgatt gatggtgtgg atattgccaa
     7981 aatatccgac gccgaactcc gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc
     8041 ctttgcctta atgccgcata tgaccgtgct ggacaatact gcgttcggta tggaattggc
     8101 cggaattaat gccgaagaac gccgggaaaa agcccttgat gcactgcgtc aggtcgggct
     8161 ggaaaattat gcccacagct acccggatga actctctggc gggatgcgtc aacgtgtggg
     8221 attagcccgc gcgttagcga ttaatccgga tatattatta atggacgaag ccttctcggc
     8281 gctcgatcca ttaattcgca ccgagatgca ggatgagctg gtaaaattac aggcgaaaca
     8341 tcagcgcacc attgtcttta tttcccacga tcttgatgaa gccatgcgta ttggcgaccg
     8401 aattgccatt atgcaaaatg gtgaagtggt acaggtcggc acaccggatg aaattctcaa
     8461 taatccggcg aatgattatg tccgtacctt cttccgtggc gttgatatta gtcaggtgtt
     8521 cagtgcgaaa gatattgccc gccggacacc gaatggctta attcgtaaaa ctcctggctt
     8581 cggcccacgt tcggcactga aattattgca ggatgaagat cgcgaatatg gctacgttat
     8641 cgaacgcggt aataagtttg tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac
     8701 gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac
     8761 gcctcttagc gagttgctct ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga
     8821 cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg
     8881 tgagggggta aataatggct gatcaaaata atccgtggga taccacgcca gcggcggaca
     8941 gtgccgcgca atccgcagac gcctggggta caccgacgac tgcaccggct gacggcggtg
     9001 gtgctgactg gctgaccagt acgcctgcgc caaacgtcga gcattttaat attctcgatc
     9061 cgttccataa aacgctgatc ccgctcgaca gttgggtcac tgaagggatc gactgggttg
     9121 ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc ggttgattat atcctcaacg
     9181 gtttccagca attgctgctg ggtatgccag cgccagtggc gattatcgtt ttcgctctca
     9241 tcacctggca gatttccggg gtcggaatgg gcgtggcgac gctggtctcg ctgattgcca
     9301 tcggcgcaat cggtgcctgg tcgcaggcaa tggtgactct ggcgctggtg ttaactgccc
     9361 tgctgttctg tatcgtcatc ggtttgccgt tggggatatg gctggcgaga agtccgcgag
     9421 cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac cacgccagcg tttgtttatc
     9481 tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca
     9541 tctttgcgct gccgccgatt atccgtctga ccattctggg gattaaccag gttccggcgg
     9601 atctgattga agcctcgcgc tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc
     9661 agttaccgct ggcgatgccg accattatgg cgggcgttaa ccagacgctg atgctggccc
     9721 tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg gctgggtcag atggtacttc
     9781 gcggtatcgg tcgtctggat atggggcttg ccaccgttgg cggcgtcggg attgtgatcc
     9841 tcgccattat cctcgatcgc ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca
     9901 accgtcgctg gtacaccact ggccctgttg gtctgctgac ccgcccattc attaagtaac
     9961 tctgcacttg cccggtgacg ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc
    10021 gacatagcgt actttttgcg acagcgtttg ccacgcttat ctctacacaa acttttgctg
    10081 ccgatctgcc gggcaaaggc attactgtta atccagttca gagcaccatc actgaagaaa
    10141 ccttccagac gctgctggtc agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac
    10201 ccagcgaagt ggattacaac gttggctaca cctcgcttgc ttccggcgat gcaaccttca
    10261 ccgccgtgaa ctggacgcca ctccatgaca acatgtacga agctgccggt ggcgataaga
    10321 aattttatcg tgaaggggta tttgttaacg gcgcagcaca gggttacctg atcgataaga
    10381 aaaccgccga ccaatacaaa atcaccaaca tcgcacaact gaaagatccg aagatcgcca
    10441 aactgttcga taccaacggc gacggtaaag cggatttaac cggttgtaac cctggctggg
    10501 gctgcgaagg tgcgatcaac caccagcttg ccgcgtatga actgaccaac accgtgacgc
    10561 ataatcaggg gaactacgca gcgatgatgg ccgacaccat cagtcgctac aaagagggca
    10621 aaccggtgtt ttattacacc tggacgccgt actgggtgag taatgagctg aagccaggga
    10681 aagatgtggt ctggttgcag gtgccgttct ccgcactgcc gggcgataaa aatgccgata
    10741 ccaaactgcc gaatggcgcg aattacggct tcccggtcag caccatgcat atcgttgcca
    10801 acaaagcctg gaccgagaaa aatccggcgg cagccaaact gtttgccatt atgcagttgc
    10861 cagtggccga tattaacgcc cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg
    10921 atattcaggg ccacgttgat ggctggatca aagcccacca gcagcagttc gatggctggg
    10981 tgaatgaggc gctggcagcg cagaagtaat ttttattcgg gcggataagg cgttttcgcc
    11041 tcatccgccg ctctgtacac aatgcctgat gcgacgctga cgcgtcctat catgcctaca
    11101 aaccacatcg cacatttcaa caagctatct ttcatcccat attcatcaac atccgctatt
    11161 attgatttcc agcttaatca tcacctgatg aacaaaaata atgactaaac ctaatcatga
    11221 gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc ggtctggcgg tagccagtaa
    11281 ctattacgcc cagccattgc tcgacaccat cgcgcgtaac ttttcccttt ccgccagttc
    11341 ggcaggcttt attgttaccg ccgcgcagtt gggctatgcc gcaggtctac tgtttcttgt
    11401 tcccctcggt gatatgtttg aacgccgccg cctgattgtc tcgatgacct tactggcagc
    11461 gggcggtatg ttaatcactg ccagcagtca gtcactggcg atgatgatcc tcggtacggc
    11521 attaaccggt ttattctcag tcgtggcaca aattctggtt ccgctggcag cgacgctggc
    11581 ttcaccggat aaacgcggca aagtggttgg caccattatg agcggtctgc tgttggggat
    11641 attgctggca cgaaccgttg ccgggttact ggcaaacctc ggcggctggc gcaccgtctt
    11701 ttgggttgct tcggtgttaa tggcactgat ggcgctggca ttatggcgtg gtctgccaca
    11761 aatgaaatca gaaacccacc tcaactaccc acagttgttg ggttccgttt tcagcatgtt
    11821 catcagcgat aagatcctgc gcacccgcgc gttgctgggc tgcatgacct ttgccaactt
    11881 cagcattctc tggacctcaa tggccttttt gcttgccgct ccacctttta actacagcga
    11941 tggtgacatt ggtctgtttg ggcttgcggg agctgccgga gcgttgggcg ctcgtccggc
    12001 gggcggtttt gccgacaagg gcaaatcgca ccacaacttt cggtctgctt ctgctgttac
    12061 tttcatggct ggcaatctgg tttggtcaca cttccgtact ggcgttgatt atcggcattc
    12121 tggtgctgga cctcaccgta cagggcgtgc atatcactaa ccagacggta atttatcgaa
    12181 tacatcctga tgcgcgtaat cgcctgaccg caggttacat gaccagctac tttattggcg
    12241 gtgccgccgg ttcgctaatt tcagcctcag cctggcaaca tggcggttgg gctggcgttt
    12301 gtctggctgg cgcgacgatt gccctggtta acttactggt ctggtggcga ggttttcatc
    12361 gtcaggaagc cgcaaattaa gcaatcagca accgttttcg tggctttaca cttataaggg
    12421 tgttaagaag cccatcagtc tgataaggtt aagatattca ttcagtctat ttataatatt
    12481 aacaatcgtt aagcgtacac tctatggaaa gccctactcc acagcctgct cctggttcgg
    12541 cgaccttcat ggaaggatgc aaagacagtt taccgattgt tattagttat attccggtgg
    12601 cctttgcgtt cggtctgaat gcgacccgtc tgggattctc tcctctcgaa agcgtttttt
    12661 tctcctgcat catttatgca ggcgcgagcc agttcgtcat taccgcgatg ctggcagccg
    12721 ggagtagttt gtgggttgct gcactgagcg tcatggcaat ggatgttcgc catgttttgt
    12781 atggcccgtc actgcgtagc cgtattattc agcgtctgca aaaatcgaaa accgccctat
    12841 gggcgtttgg cctgacggat gaggtttttg ccgccgccac cgcaaagctg gtacgcaata
    12901 atcgccgctg gagcgagaac tggatgatcg gcattgcctt cagttcatgg tcatcgtggg
    12961 tatttggtac ggtaataggg gcattctccg gcagcggctt gctgcaaggt tatcccgccg
    13021 ttgaagctgc attaggtttt atgcttccgg cactctttat gagtttcctg ctcgcctctt
    13081 tccagcgcaa acaatctctt tgcgttaccg cagctttagt tggtgccctt gcaggcgtaa
    13141 cgctattttc tattcccgtc gccattctgg caggcattgt ctgtggctgc ctcactgcgt
    13201 taatccaggc attctggcaa ggagcgcccg atgagctatg aggttctgct gcttgggtta
    13261 ctggttggcg cggcgaatta ttgcttccgc tatttgccgc tgcgcctgcg tgtgggtaat
    13321 gcccgcccaa ccaaacgtgg cgcggtaggt attttgctcg acaccattgg catcgcctcg
    13381 atatgcgctc tgctggttgt ctctaccgca ccagaagtga tgcacgatac acgccgtttc
    13441 gtgcccacgc tggtcggctt cgcggtactg ggtgccagtt tctataaaac acgcagcatt
    13501 atcatcccaa cactgcttag tgcgctggcc tatgggctcg cctggaaagt gatggcgatt
    13561 atataaccca caagaatcat ttttctaaaa caatacattt actttatttg tcactgtcgt
    13621 tactatatcg gctgaaatta atgaggtcat acccaaatgg atagttcgtt tacgcccatt
    13681 gaacaaatgc taaaatttcg cgccagccgc cacgaagatt ttccttatca ggagatcctt
    13741 ctgactcgtc tttgcatgca catgcaaagc aagctgctgg agaaccgcaa taaaatgctg
    13801 aaggctcagg ggattaacga gacgttgttt atggcgttga ttacgctgga gtctcaggaa
    13861 aaccacagta ttcagccttc tgaattaagt tgtgctcttg gatcatcccg taccaacgcg
    13921 acgcgtattg ccgatgaact ggaaaaacgc ggttggatcg aacgtcgtaa aagcgataac
    13981 gatcgccgct gcctgcatct gcaattaacg gaaaaaggtc acgagttttt gcgcgaggtt
    14041 ttaccaccgc agcataactg cctgcatcaa ctctggtccg cgctcagcac aacagaaaaa
    14101 gatcagctcg agcaaatcac ccgcaaattg ctctcccgtc tcgaccagat ggaacaagac
    14161 ggtgtggttc tcgaagcgat gagctaacgc gtcatctcgc tcaaaaatcc agatttataa
    14221 aagaaaaaat gaccggccag catcgcaaca tgctggcctt tttggcaagc aggtcggctc
    14281 agccgatgag ttaagaagat cgtggagaac aatatgagcg caaatgcgga gactcaaacc
    14341 ccgcagcaac cggtaaagaa gagcggcaaa cgtaagcgtc tgctcctcct tctcaccttg
    14401 ctctttataa ttattgccgt agcgataggg atttattggt ttttggtact gcgtcatttc
    14461 gaagaaaccg atgacgcata cgtggcaggg aatcagattc aaattatgtc tcaggtgtct
    14521 ggcagcgtga cgaaagtctg ggccgataac accgattttg taaaagaagg cgatgttctg
    14581 gtcactctcg acccgacaga tgctcgccag gcgtttgaaa aagccaaaac tgcactggct
    14641 tccagcgttc gccaaaccca ccagctgatg attaacagca aacagttgca ggcgaatatt
    14701 gaggtgcaaa aaatcgccct cgcgaaagca caaagcgact acaaccgccg tgtgccgctg
    14761 ggcaatgcca acctgattgg tcgcgaagag ctgcaacacg cccgcgacgc cgtcaccagt
    14821 gcccaggcgc aactggacgt cgctattcaa caatacaatg ccaatcaggc gatgattctg
    14881 gggactaaac tggaagatca gcctgccgtg caacaggctg ccaccgaagt acgtaacgcc
    14941 tagctggcgc tggagcgtac ccgtattgtc agtccgatga ccggttatgt ttcccgtcgt
    15001 gccgtgcaac cgggtgcgca aattagccca acgacgccgc tgatggcggt cgttccagcc
    15061 accaatatgt gggtggatgc caactttaaa gagacgcaga ttgccaatat gcgcatcggt
    15121 cagccggtca ccatcaccac cgatatttac ggcgatgatg tgaaatacac cggtaaagtg
    15181 gttggtctgg atatgggcac aggtagcgcg ttctcactgc ttccggcgca aaatgcgacc
    15241 ggtaactgga tcaaagtcgt tcagcgtcta cctgtgcgta tcgaactgga ccagaaacag
    15301 ctggagcaat acccgctgcg cattggtttg tccacgctgg tgagcgtcaa taccactaac
    15361 cgtgacggtc aggtactggc aaataaagta cgttcaacac cggtcgcggt aagtaccgcg
    15421 cgtgaaatca gcctggcacc tgtcaataaa ctgatcgacg atatcgtaaa agctaacgct
    15481 ggctaatcca gaggtgcgtg tgatgcaaca gcaaaaaccg ctggaaggcg cgcaactggt
    15541 cattatgacg attgcgctgt cactggcgac attcatgcag gtgctgaact ccaccattgc
    15601 taacgtggcg atccccacta tcgccgggaa tctgggctca tcgctcagcc aaggaacgtg
    15661 ggtaatcact tctttcgggg tggcgaatgc catctcgatc ccgcttaccg gctggctggc
    15721 aaagcgcgtc ggggaagtga aactgttcct ttggtccacc atcgcctttg ctattgcgtc
    15781 ttgggcgtgt ggtgtctcca gcagcctgaa tatgctgatc ttcttccgcg tgattcaggg
    15841 gattgtcgcc gggccgttga tcccgctttc gcaaagtcta ttgctgaata actacccgcc
    15901 agccaaacgc tcgatcgcgc tggcgttgtg gtcgatgacg gtgattgtcg cgccaatttg
    15961 cggcccgatc ctcggcggtt atatcagcga taattaccac tggggctgga tattcttcat
    16021 caacgtgccg attggcgtgg cggtggtgtt gatgacactg caaaccctgc gcggacgtga
    16081 aacccgcacc gaacggcggc ggattgatgc cgtggggctg gcactgctgg ttattggtat
    16141 cggcagcctg cagattatgc tcgaccgcgg taaagagctg gactgttttt catcacagga
    16201 aattatcatt cttaccgtgg tggcggtggt ggctatctgc ttcctgattg tctgggagct
    16261 gaccgacgat aacccgatag tcgatctgtc gttgtttaag tcgcgcaact tcaccatcgg
    16321 ctgcttgtgt atcagcctcg cgtatatgct ctacttcggc gctattgttc tgctgccgca
    16381 gttgttgcag gaggtctacg gttacacggc gacctgggca ggtttggcct ctgcgccggt
    16441 agggattatt ccggtgattc tgtcgccgat tatcggccgc ttcgcgcata aactggatat
    16501 gcggcggctg gtaaccttca gctttattat gtatgccgtc tgcttctact ggcgtgccta
    16561 tacctttgaa ccaggtatgg atttttgcgc gtcggcctgg ccgcagttta tccaggggtt
    16621 tgcggtggtc tgcttcttta tgccgctgac caccattacg ctgtctggtt tgccaccgga
    16681 acgactggcg gcggcatcga gcctctctaa ctttacgcga acgctggcgg ggtctatcgg
    16741 cacgtcgata accacgacca tgtggaccaa ccgcgagtcg atgcaccatg cgcagttgac
    16801 tgagtcggta aacccgttca acccgaatgc ccaggcgatg tacagtcaac tggaagggct
    16861 tgggatgacg caacagcagg catcaggctg gattgcccag cagatcacca atcaggggct
    16921 gattatttcc gccaatgaga tcttctggat gtcagccggg atattcctcg tactgctggg
    16981 gctggtgtgg tttgctaaac cgccatttgg cgcaggtggc ggcggaggcg gtgcgcacta
    17041 agtacaacta agccagttca tttgaactgg cttttttcaa ttaattgtga agatagttta
    17101 ctgactagat gtgcagttcc tgcaacttct ctttcggcag tgccagttct tcgttgctgt
    17161 tgatgcgtac ctcacgttcc agaatgctac gcgcaatatc ctgcgcttcc tgcaacgagt
    17221 gcatctggta agtgccacac tggtagacgt tcagttccgg gatctgattc tgatcctgca
    17281 ctttcagcac gtcttccatt gccgctttcc aggcatcagc aacacgctgc tcatctggcg
    17341 taccaatcag actcatataa aaaccggtgc ggcagcccat tggcgagata tcgataatct
    17401 ctacaccatt accgttaaga tggttacgca taaaaccagc aaacaggtgc tccagggtat
    17461 ggatccctct ttctggcatc acttctttgt tcggcacgca gaagcgcaga tcgaacacgg
    17521 tgattgcgtc gccatgcggg gtgttcattg ttttcgccac ccgaactgca ggcgcttcca
    17581 tccgggtatg atcgactgtg aagctatcta acaacggcat ttagccacct ccggtaattt
    17641 ttttaaaaat tttctgaact ctttcttccc aggcgagtct gagtatatga aagacgcgca
    17701 tttgttatca tcatccctga attcagagat gaaattttgg ccactcccga gtggcctttt
    17761 tcttttctgt caggcgtgtt tttccagcca caccgcaaac ggttcggtat cagcggtttc
    17821 catttcctgc tgacggcgtt cagatgcctc gcgctcggct acaaaatcct cttcgcgcag
    17881 aatttccagc ggctcttcac gcagcagatt acggtaggct tctgcaaacg ctttgcctgt
    17941 tccgccaata ccagtatcaa tcatagacct taagatacgg gcagagaaag tcagatcggg
    18001 attatcgaag caggcaacca gttcatcaca cactttctga tacgcttcgc cgccgttaat
    18061 gctatccagc gtttgcgcga cgcgtttcag atcgcggaac agatctttac ccacctgcag
    18121 taacgggaac tgcgcggttt cgcagccgat acccagcgtc aggcccggtt tgcgaccttc
    18181 aaggatcacc cggttccagt taacgcgcgt acaggcaagt tcgctactgc tcatttccgg
    18241 tgcatcagcc agcgcacacc agaccataaa caggtcgagg aatcgcacct gctgttcatc
    18301 tacaccaatc ggcgagaacg ggttgatgtc cagcgaacgc acttcaatat attcaatgcc
    18361 gccacgtaac agcgcatcag aaggtgactc gccgctgcgg gtaacgcgtt ttggacgaat
    18421 cggcgcatac agttcgtttt caatctgcag cacgttgcta ttgatttgca gcctcttacc
    18481 gtctttctca ataccaatct tcgcgtactc ttccgatggc gttttgattg cctgtttaag
    18541 gcccgctacg tactcgtaaa gatcgttgaa ggtaatacca agattgcttt gcgatttatt
    18601 ggtatagccg agatcgctca aacgaagaga ggtcgcatac ggcaggtaat acataccgca
    18661 ctcggttttc tcaaacggca gcgacgttgg ttttccttgc aggaaagaag aacaaatcgc
    18721 cggagatgca ccaaacagat aaggaataac ccaaccgaaa cgatagtaat tgcggataac
    18781 gcggaaatag cccgcagaaa ttttctcttt ggcatcagcg cccgagatat caccgcactt
    18841 cgcttgccag aatgccattg gcaaagagaa attgtagtgc acgccggaaa tggtttgcat
    18901 cagcgcgccg tagcgatttt tcagcccttc acgatacagc gttttaaagc gtccggtgtt
    18961 agaagtgccg tactgtgcca gttcgatgtc ctgaccttct gcgatgtagc atggcatact
    19021 taacggccac atccgctcat cgcccatatt gcgcgccgta taacgatgca gatcgcgcat
    19081 aaaggtcagc atatgttcaa tatcaccatc cactggtgta atgaattcca gcaatgcttc
    19141 cgcaaaatcg gtagtaatcc atttgtgcgt cagtgcggaa cctaatgctt caggatgacc
    19201 tgttgttgcc agtgtgccat cagcattaac acgcaaagtt tcgcgctcca gcccacgctg
    19261 tatccccttt aacgcctgag gatgtttttc cagccaggcc agcgcctgtg atacgtccgg
    19321 gatcaaattg acctcccgcc tgtcaaaatc gttttaatta gcataactgt aatggtgacc
    19381 atatgtgcag gcctacaatt agtgccacca catcatgccc tgaacggtcg ctgctgcaac
    19441 tgcaacatag cgtaacgctt taccaaggca caaaaaaaag attactggcc cccaggagat
    19501 gcgcatccat cccgctaaca gacacagtaa atcgccaacc acgggcatcc agcttaataa
    19561 tagcgtgact gcaccatagc gtttcagcca gccggtagct ttctcttgcc agcgcgatgt
    19621 tttacgcaat ggaaagaaac gcccaaggat aacgttagtt aaccctccaa ggctattacc
    19681 cattgttgct gttaaaacta aaacccaggg atgactgatc ccggaaagca acattgccac
    19741 cagcacgact tcggagttgc cgggtaatag tgtagcgctg agaaaactac tggcaaacaa
    19801 cgagaaaagc gataacgctt cactcacagc aagcgaacat ccacagcgtc catgcctgct
    19861 gcacgggccg cctgaatacc gaaatcggca tcttcaaaga ccacacactg cgtcggttgc
    19921 acgcccatac gctgcgcgca caacaaaaat gtgtctggcg cgggtttatg gtgtttgacg
    19981 tgatcagcag cgacgacggc gtcaaaataa cggcgtaatc ccaggtgcgc cagcaatgcc
    20041 tcagcgatgg cgctttcact ccccgttcct acagccattg ggcgacgacc atgccaactt
    20101 ttcaccactt caacaagagg aagcggctcg acactatcca gcagcatact tcttactgct
    20161 tctgtttttt cacgcgctaa cgcatgcggg tcgagatcgg cctgattcag ctcaataatt
    20221 gcctgagcaa tacgccaggt gggcgatcca ttaagcgcaa tcatcgcctg aatatcgtac
    20281 tgaagaccgt agtgccctaa tacttcgcgc cacgctttac ggtgcgtagg cgccgtatcc
    20341 aggattgtgc catccatatc aaaaattaaa cctgcataac gctcgtacat ggtcttctcg
    20401 caagtcgaaa aatatgacgc tactttagcg taattgcttg attttgtcgc tgatggcgaa
    20461 ggagaagaaa attcaggaaa tggataaagt agttatttag aaatgagata tttttgaagg
    20521 aaattttttg gagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
    20581 agccgagtac tctatccagc tgagctacgg atacatcggg aaacttactt tactgcagat
    20641 tttttgatac cgctactaaa gccgtatcaa gtaagagatg gtgcatccgg gaggattcga
    20701 acctccgacc gctcggttcg tagccgagta ctctatccag ctgagctacg gatgcaaatg
    20761 gcggtgaggc ggggattcga accccggatg cagcttttga ccgcatactc ccttagcagg
    20821 ggagcgcctt cagcctctcg gccacctcac cacacgcctc ttacgagtgc ttcgaagaac
    20881 ttgtttatcg ctcatcatcg ctgcgtggcg cacatattac tttctgagac ttataagtca
    20941 aacaattttt cccacacttt tatcgtttgc acacttcacg ctcaattagt ctgtaaaagc
    21001 gtcaaaaaga gtgttttatc tacagaaaaa cggaggtctg gcagatagca gtaatgcaaa
    21061 aaaatggaga cttaagttga atgaacggga gtaaagcgaa aagactatag agtgaaggag
    21121 aaattttgag ggtgcgtctc accgataaag atgagacgcg gaaagattag taactggact
    21181 gctgggattt ttcagcctgg atacgctggt agatctcttc acggtgaaca gaaacttcct
    21241 tcggggcatt tacgccaata cgtacctggt tgccctttac ccctaaaact gtcacggtga
    21301 cctcatcccc aatcatgagg gtctcaccaa ctcgacgagt cagaatcagc attctttgct
    21361 ccttgaaaga ttaaaagagt cgggtctctc tgtatcccgg cattatccat catataacgc
    21421 caaaaagtaa gcaatgacaa acacattaca tgtaagcagt catggcatta cattctgtta
    21481 aacctaagtt tagccgatat acacaacttc aacctgactt tatcgttgtc gatagcgtta
    21541 atgcgaatgc cgtgaagcga gtccacggca ttgcctgacg cttatattat tgcaatttcg
    21601 cgctgaccca gcctttcaca ctggctaacg ctgcaggtaa ggccgcagca tccgtaccac
    21661 cggcttgcgc catgtcagga cgtccaccac ccttgccgcc cacctgctga gcgaccatac
    21721 caatcagttc ccctgctttc acacgatctg tgacgtcctt agatacgcct gcaatcagag
    21781 aaaccttacc ttcggctacc gttgccagca cgataattgt cgaccccagc tgatttttta
    21841 aatcgtcaac catggtacgc aacattttcg gctcaacacc gctaagctcg ctaaccaaca
    21901 gcttaacacc attaacatca attgccttac tggaaagatt tgcgctctcc tgtgcggcag
    21961 cttgttcttt aagctgttgt agttcttttt ctaactgacg cgtacgttcc agtactgagc
    22021 gcactttatc cgccagatta ttgctatcgc ctttcagcag atgcgcgact tcgcttaagc
    22081 gatcgctgtc tgcatgaacg gtggtgatag cgccttctcc ggttaccgct tcgatacgac
    22141 gaacgcctgc agcagtaccc gattcagaga tgatgcggaa cagaccaata tcaccagtgc
    22201 ggctggcgtg agtaccgcca cacagctcgg tggagaaatc gcccatgctc agcacgcgta
    22261 cacgctcatc atacttctcg ccgaacagcg ccatcgcacc tttcgctttc gccgcttcga
    22321 gattcatgat gttggtttcg atcggcaaat tgcgacgaat ctgtgcgttc accaggtctt
    22381 cgaccgcacg aatctcttcc ggtttcatcg cttcgttgtg tgagaagtcg aagcgcagca
    22441 ccttgtcgtt aaccagtgaa cctttctgcg atacatgagt ccccagaacc tggcgcagcg
    22501 cagcgtgcat caggtgcgtt gcggagtgat tcagacgaat acgggcgcga cgagcctcat
    22561 caatatcagc ctgcacagcg tcgcccactt tcagagaacc cgcagcaagt ttaccgatgt
    22621 gaccaatcgc ctggccgtat ttctgcgtat cttccaccgc gaaggagaag ttagcgcctt
    22681 tcagttcgcc tttatcacca acctgaccgc cggattccgc atagaatggc gtttgatcca
    22741 gcacgaccac agcttcctgg ccggcattga tggcatcaac cgctttacca tcaacaaaca
    22801 gcgcggtcac tttgccgttc agttccagat ggtcatagcc tttaaattca gatgcactat
    22861 caacacggat cattgcgttg taatcggcac caaagccgct ggcttcgcgc gcgcgacgac
    22921 gctgctcttc cattgctgct tcaaaaccag cttcgtcaac tttgatgttg cgctcacgac
    22981 aaacatcagc cgtcaggtca accgggaagc cataggtgtc gtacagacgg aaagccgttt
    23041 caccatccag cgtatcacca gaaagttttg ccagctcttc atccagcaac gccagaccgc
    23101 gctccagagt acgggcaaac tgctcttctt cagtcttcag cacctgctca acctgcgcct
    23161 gctggcgttt caggtcttca cccgcagagc ccataacgtc gatcagcgga ccaaccagtt
    23221 tgtagaagaa ggtttctttc gcgccgagca tattgccgtg acgcactgcg cgacgaatga
    23281 tacgacgcag tacataacca cggttttcat tggacggcat tacgccatcc gcgatcagga
    23341 acgcacaaga acgaatgtgg tcagcgatta cgcgcagcga tttattgctc agatcggtcg
    23401 cgccagtgac tttcgctacc gcctggatca acgtgcggaa caggtcgatg tcatagttag
    23461 agttaacgtg ttgcagcacc gcagcaatac gctccagacc cataccggta tctacagacg
    23521 gcttcggcag cggttccatc gtgccatctg cctggcggtt gaactgcatg aagacgatgt
    23581 tccagatctc aatgtagcgg tcgccgtctt cttccggact tcccggaggg ccaccccaaa
    23641 tgtggtcgcc gtgatcgtag aagatttcgg tgcacgggcc gcacggacca gtgtcaccca
    23701 tctgccagaa gttgtcagat gcgtatggcg cacctttgtt atcgccgatg cgaataatac
    23761 gttcgcgcgg gatcccgact tctttttccc agatttcgta ggcttcgtcg tcgctttcat
    23821 agacggtaac ccacagacgc tctttcggca gggcaaacca tttttcgctg gtcagcagtt
    23881 cccatgcaaa ctgaatggca tcgtgtttga aatagtcgcc gaagctgaag ttgcccagca
    23941 tttcgaagaa ggtatggtga cgcgcggtgt aaccgacgtt ttccaggtcg ttgtgtttac
    24001 cacccgcacg cacgcagcgt tgggaagtgg tagcgcggga ataattacgc ttgtcgagcc
    24061 caaggaacac atccttgaac tggttcatcc cggcgttggt aaacaacaaa gttgggtcgt
    24121 tatggggtac cagggagctg ctggcaacta cctgatgtcc cttactatgg aaaaagtcga
    24181 gaaacgcctg acggatctca gcggtgctct tgctcataat tatcctgaaa tcaagctaac
    24241 gaaatatcgc caccagctcc agcgtgtctt aaccgccggg ctggtaactg aaaagtggga
    24301 ataagataag ttttcttgac tgggaagtaa aataccgtat gcgttcagtc ggcaaaattt
    24361 cgccaaatat cctggatatc ttccatcaga tagccacgat agagcagaaa acgctggatc
    24421 ttaacttttt ctgaaaagac agttggcaaa ggttcaccat attttcgcgt cgcctgatcg
    24481 cgcgccagtg cgcaccagtc gatgtcacat tcacgcatcg ctttttctgt cgcttcgcgg
    24541 gaaatacctt tctgattcag ttcctgacga atacgtgcag gtccataccc tttgcggcta
    24601 cggctggcga taaagcgcgc aacaaatcgg ctgtcatcga gatagccatg ttcatggcac
    24661 caggcaataa cgcgctcgta atcttctgcc gtagcatcaa tctcttctgg gccatttttg
    24721 cccataatcg gtgccgcgag tttacgtcgc agttcttgct cactgtgatc gcgcaccgcc
    24781 agaatgcgta ccgcacgatc caacaggcga gcatatgccg ggcgacggga tgttgattct
    24841 gtcatggcat atccttacaa cttaaaaaag caaaagggcc gcagatgcgc cccaggtttt
    24901 gctgataaac agatttaata gtcaatgagt atgcagaatg ttcccgcagg cccaattcgt
    24961 tagaggatcg aggtgagagt aatttttcac gccagtgctg gcctgttccc ctcaccctaa
    25021 ccctctcccc aaaggggcga ggggaccgtc cgtgcacaca ttatctatta tcatcaataa
    25081 cgaacgatta aaaatcttcg ttagtttctg ctactccttc gctgtcatct acagagaaat
    25141 ccggcgttga gttcgggttg ctcagcagca actcacgtac tttcttctcg atctctttcg
    25201 cggtttccgg gttatctttc agccaggcag ttgcattcgc tttaccctga ccgatcttct
    25261 cacctttgta gctgtaccac gcgcctgctt tctcgatcag cttctctttt acgcccaggt
    25321 caaccagttc gccgtagaag ttgatacctt cgccgtagag gatctgaaat tcagcctgtt
    25381 taaacggtgc agcgattttg ttcttcacca ctttcacgcg ggtttcgcta cccaccacgt
    25441 tttcgccctc tttcaccgcg ccgatacgac ggatgtcgag acgaacagag gcgtagaatt
    25501 tcagcgcgtt accaccggta gtggtttccg ggttaccgaa catcacacca attttcatac
    25561 ggatctggtt gatgaagatc agcagcgtgt tggactgctt caggttaccc gccagcttac
    25621 gcatcgcctg gctcatcata cgtgccgcaa ggcccatgtg agagtcgccg atttcgcctt
    25681 cgatttccgc tttcggcgtc agtgccgcca cggagtcaac gacgataacg tctactgcgc
    25741 cagaacgcgc cagggcgtca cagatttcca gtgcctgctc gccggtatcc ggctgggagc
    25801 acagcaggtt atcgatatcg acgcccagtt tacgtgcgta gattgggtcc agcgcgtgtt
    25861 cagcatcgat aaacgcacag gttttacctt cacgctgcgc tgcggcgatc acctgcagcg
    25921 tcagcgtggt tttaccggaa gattccggtc cgtagatttc gacgatacgg cccatcggca
    25981 gaccacctgc cccaagcgcg atatccagtg aaagcgaacc ggtagagatg gtttccacat
    26041 ccatggaacg gtcttcaccc aggcgcatga tggagccttt accaaattgt ttctcaatct
    26101 ggcccagtgc tgccgccaac gctttctgtt tgttttcgtc gatagccatt tttactcctg
    26161 tcatgccggg taataccgga tagtcaatat gttctgttga aacaattata ctgtatgctc
    26221 atacagtatc aagtgttttg tagaaattgt tgccacaagg tctgcaatgc atacgcagta
    26281 gcctgacgac gcaccgcatc acggtcgccg ctgaagcatt cccgccgggt aatgccttca
    26341 ccgcgggcag tggcaaaagc aaaccagacg gtgccgacag gcttctcttc actgccgcca
    26401 tccggcccgg cgataccact aatagacacg gcataatcag cacgagccgc tttcagtgca
    26461 cctatcgcca tttccaccac gacgggttca ctcaccgcgc catgctgcgc cagcgtctct
    26521 tcgcgtacgc cgatcatctg cgctttggct tcgttactgt aggtgacaaa tccgcgttca
    26581 aaccaggcgg agctaccggc aatatcggta atcactttcg ctacccaacc accggtacaa
    26641 gactcggcag ttgttacggt tgcgccacgg gctttcagcg cctgcccaac ctgttcgctt
    26701 aactgcatca gttcactgtc agtcatcaca acctctgtca gtcaaaaatt tatgccggac
    26761 aagatagcac tttcgtcagg aagatggtgt tggcgttgca attttacgag gaggattcag
    26821 aaaaaagctg attagccaga gggaagctca cgcccccctc ttgtaaatag ctactgtact
    26881 cgcgccagcg ccacggcttg tcctaactgc cagaccgcca ttgcgtaatg ggtgctgtgg
    26941 ttgtaacggg tgatggtgta gaagttcggc agaccgtacc agtactggta gccggtgcca
    27001 acatccagac gcagcaggct ggcttgttga tggttgccca gcggctgctg tggcgttaaa
    27061 cctgcggcgg caagctgcga aatgctgtac ttagttttga aaccatttgg caagcctgga
    27121 gcctgaccgt ttgccattat cgcgacctga tcgcctttca cccagccgtg cgctttgaaa
    27181 tagttcgcca cgctaccgat cgcatcaacc ggatcccaca ggttgatatg cccgtcgccg
    27241 ctgaaatcta ccgcatattg tttgtaagac gacggcataa actgtccgta gcccatcgcc
    27301 ccggcaaagg aacctttcag attgagcgga tcgtcctgct cgtcgcgcgc catcagcagg
    27361 aaggtttcca gctcgccaga gaaatactcc gcgcggcgtg ggtagttaaa tgacagcgtt
    27421 gccagcgcat cgaggatgcg agttttcccc atcacgcgcc cccagcgggt ttcaacgccg
    27481 ataatcccga cgataatttc cggcggtacg ccatacacct gccacgcgcg attcaacgca
    27541 tcttcatact gattccagaa aaccacaccg ttctgcacgt tgtccggcgt aataaatttt
    27601 ttgcgataac gcagccatgc gccgttcgga cctgatggcg gtttcaccga tgtggttggt
    27661 gcctggttat ccatcagccg cagtaccgaa tccagacgct tcgcctggga gagaatttcc
    27721 tgcaactgct gacgatcgaa accgtgtttg ttcaccattt tgtcgatgaa ctgctgggca
    27781 ttcgggttat tagcgaaatc gccgcccatc tgcatcacat tgtgctgcgg ctcaagcagg
    27841 aagccgccag acggcgttcc ggtggtcgtt tcagtctcag taggttttgg cttgctgcta
    27901 caggcggcaa gcaacacaaa aaggggaagc aatgttacat aacgacgctt gaacatgagg
    27961 ggtccattta acagattcaa ccaggggcaa gtatggtaaa gcatcacacc ccgcacaagg
    28021 aagcggtagt cactgcccga tacggacttt acataactca actcattccc ctcgctatac
    28081 ttttattcaa actttcaaat taaaatattt atctttcatt ttacgatcaa aataacactt
    28141 ttaaaccttt caacctgatt agattaggtt gccgtttggt aataaaacaa taaatcctga
    28201 aggagagaac aatgatagaa accattactc atggtgcaga gtggtttatc gggctgttcc
    28261 aaaagggcgg agaggtgttt accgggatgg tgaccggcat tcttccgctg ttgattagcc
    28321 tgcaggttat catgaacgca ctgattaatt ttatcggtca gcatcgtatt gaacgttttg
    28381 ctcaacgttg cgccggtaac cctgtttccc gttacctact gttaccgtgc attggcacgt
    28441 ttgtcttttg caatccgatg accttaagcc tgggtcgctt tatgccggaa aagtacaaac
    28501 ccagctacta cgcggcggcc tcttatagct gccactcaat gaatggcctc ttcccccata
    28561 tcaaccctgc gaactgtttg tttatcttgg cattgccagc ggtctgacaa cgctgaacct
    28621 gccacttggc ccactggcgg tgagttatct gctggttggt ctggtcacca atttcttccg
    28681 cggctgggtg accgatctga ccaccgccat ttttgagaaa aagatgggca ttcaacttga
    28741 acaaaaagtt cacctggcag gagcaacatc atgacgcgta ttcggatcga aaaaggaacg
    28801 ggtggctggg gcggcccgct tgagctggaa gccacgccgg gcaaaaaaat cgtctatatc
    28861 accgccggta cccggcctgc gattgttgac aaactggcac aacttactgg ctggcaggct
    28921 attgacggat ttaaagaagg tgaacccgcg gaggcggaaa ttggtgtcgc ggtaatcgac
    28981 tgtggcggca cattacgctg cggcatctat ccgaaacgac gtattcccac cattaatatc
    29041 cactcgacgg gcaagtccgg tccgctggcg cagtacattg tggaagatat ttatgtctct
    29101 ggcgtaaaag aagaaaacat cactgtagtg ggtgatgcga caccacaacc ctcttccgtg
    29161 ggccgtgact atgacaccag taagaaaatc accgaacaaa gcgatggttt actggcgaag
    29221 gtgggaatgg gcatggggtc cgccgttgcc gtgttgtttc agtctggtcg tgacaccatc
    29281 gacactgtat taaaaaccat tctgccgttt atggcattcg tctcggcgct cattggcatc
    29341 attatggctt ctggccttgg tgactggatt gcccacggtc ttgctccgct agcgagccat
    29401 ccactgggtc tggtcatgct ggcgctcatc tgctccttcc cactgctttc acctttcctc
    29461 ggcccaggcg cagttatcgc acaggttatc ggcgtattga ttggcgtgca gattggtctc
    29521 ggcaatattc cgccgcatct ggctttaccg gcactgtttg ccatcaacgc gcaggcggct
    29581 tgcgacttca tcccggtcgg tttgtcgctg gcggaagccc gtcaggacac ggttcgcgtc
    29641 ggtgtccctt ctgtactggt gagccgcttt ttaaccggcg cgccgactgt actgatcgcc
    29701 tggtttgtct ccggttttat ctatcaatag aggctgaaac atgaccgtta tttatcagac
    29761 caccatcacc cgtatcggcg cgagtgccac tgacgccctc agcgaccaga tgctcatcac
    29821 ctttcgtgaa ggcgcgccag cggacctcga agagtattgc ttcattcatt gccacggcga
    29881 gttgaaaggt gcactccatc ccggtttgca attttcactc gggcagcatc gctatccggt
    29941 gaccgctgtt ggcagcgtgg cggaagacaa ccttcgcgaa ctgggtcatg tcaccctgcg
    30001 cttcgatggt ttaaacgaag cggaatttcc gggcactgtc catgtggcag gccctgtacc
    30061 cgacgatatc gcgccgggat cggttttgaa gtttgaatct gttaaggagt aaaaaatgaa
    30121 tcaggttgcc gttgtcatcg gtggtgggca aaccttaggc gcgttcctgt gccacggtct
    30181 ggctgccgag gggtatcgcg tcgcggttgt cgatattcag agcgacaaag ccgcaaatgt
    30241 ggcacaagaa attaacgccg aatatggtga aggtatggcg tacggttttg gtgctgacgc
    30301 cactagcgag caaagcgttc tggcgctctc tcgtggggta gatgaaatct ttggtcgcgt
    30361 ggatttgctg gtctatagcg ccggaatagc aaaagcagcc tttatcagcg acttccagct
    30421 cggcgatttt gaccgttcgc tacaagtgaa tctggtgggt tatttcctgt gtgcccgtga
    30481 attttcgcgt ttgatgatcc gcgacgggat tcagggtcgc attattcaga tcaactcgaa
    30541 atccggcaaa gtgggcagca aacacaactc tggctacagc gcagcgaaat ttggtggcgt
    30601 cgggctgact caatcactgg cgctggatct ggcggagtac ggcattacgg tgcattcact
    30661 gatgctcggt aacctgctga aatcgcggat gttccagtca ctgttgccac aatacgcgac
    30721 caagctgggt atcaaaccgg atcaagtcga gcagtattac atcgacaaag taccgctcaa
    30781 acgcggctgc gattatcaag atgtgctgaa tatgctgctg ttctacgcca gtcctaaggc
    30841 gtcgtactgc accggacagt cgatcaatgt caccggcggt caggtgatgt tctgatcaac
    30901 agcggagatc cgttaaggat ctccgtgaga ctatagaatg cctgatgcgc tacgctcatc
    30961 aggcatacag gacttccacc actacattaa ggaaaagtta tggtatccgc actcatcacc
    31021 gtcgccgtta tcgcctggtg tgcgcaactg gccttaggcg gctggcaaat ttctcgtttt
    31081 aaccgtgcct tcgacacact atgccagcaa gggcgggttg gcgtggaccg ttccagcggg
    31141 cgctttaaac cgcgggtcgt ggtcgccatc gcgctggacg atcagcagtg catcgtcgac
    31201 accttgttta tgaaaggact gactgtcttc gcccgaccgc aaaaaattcc cgcaattacc
    31261 ggcatgcatg cgggtgattt acagcccgat gtgatctttc cccatgatcc actatcacag
    31321 aatgctctat cattggcgct taaactgaaa cgtggataat ttcgttgtga atgttacttg
    31381 cttgcgaagt tatcattttg aaacctaaat caggtaatca cgcccatgaa acctcgtcag
    31441 cgtcaggccg ccattctgga gtatctgcaa aagcagggta aatgctcggt tgaagaattg
    31501 gcgcaatact ttgacaccac aggcacaacc attcgcaaag atctggtcat tctggaacat
    31561 gccggaaccg tcattcgtac ttatggcgga gtggtgttga ataaagagga atccgatccg
    31621 cctatcgatc ataaaacgct catcaacacc cacaagaaag agctgattgc agaagctgcc
    31681 gttagtttta tccatgatgg cgattcgatc attcttgatg ctggcagtac cgttttgcag
    31741 attgttcccc tgctctcgcg ctttaataac atcacggtga tgaccaacag cctgcatatc
    31801 gtcaatgcgc tatccgaact ggataacgaa caaactatcc tgatgccagg cggaacattt
    31861 cgcaaaaaat cggcctcatt tcacgggcaa ctggcagaga atgccttcga gcatttcacc
    31921 ttcgataaat tgtttatggg caccgacggc atcgatctca atgcgggcgt aaccacgttc
    31981 aacgaggttt ataccgtcag taaggcaatg tgcaatgccg cgcgcgaagt gattttgatg
    32041 gcggactcat caaagtttgg ccgtaaaagc cccaacgtag tttgcagtct tgaaagcgtc
    32101 gataagctaa ttaccgacgc aggtatcgat ccggcgtttc gtcaggcgct ggaagagaaa
    32161 gggatcgatg tgatcataac cggagagagc aatgagtgaa gcactactga acacgggacg
    32221 tcagacgtta atgctggagt tgcaggaagc aagccgttta ccggaacgtc tgggcgatga
    32281 ttttgttcgc gccgccaata tcatcctgca ctgcgaaggc aaagtggtgg tttcgggaat
    32341 tggcaaatcg ggccacattg gtaagaaaat cgccgcaaca cttgccagta ccggcacacc
    32401 ggcttttttt gtccatccgg cagaagcact gcacggcgat ctggggatga ttgaaagccg
    32461 cgatgtgatg ctgtttatct cttactccgg tggcgcgaag gaactggatc tgattattcc
    32521 gcgtctggaa gataaatcta tcgcgctgct ggcgatgacc ggcaaaccga cgtcaccgct
    32581 gggcctggcg gcgaaagcgg tgctggatat ctccgtagaa cgcgaagcct gcccgatgca
    32641 ccttgcgccg acctccagca ccgtcaatac cctgatgatg ggtgacgcgc tggcgatggc
    32701 ggtcatgcag gcgcgcggtt ttaatgaaga agattttgcc cgctcccacc cagctggggc
    32761 actgggcgct cgcttgctga ataaagtgca tcatctgatg cgtcgtgatg acgctatccc
    32821 acaggtagcg ttaaccgcca gcgtgatgga tgcgatgctg gaactcagcc gcaccggtct
    32881 ggggctggtg gcggtatgtg acgctcaaca acaggtacaa ggcgtcttta ccgacggcga
    32941 tttacgtcgc tggctggtag gcggcggcgc actcaccacg ccagttaatg aagcgatgac
    33001 gacaggcggc accacattac aggcacaaag tcgcgccatc gacgccaaag aagttctgat
    33061 gaaacgcaaa atcactgccg caccagtggt agatgaaaac ggcaaactca ccggcgcaat
    33121 taacctgcag gatttctatc aggccgggat tatttaatcc ttcaatccca gacgtttcgc
    33181 cagccgatgc aggttggcga cgtcagtttc cagcattcgc gcacaggctg cccagttgtg
    33241 atgattttgt gccagtgcct ggcgaatagt ttcacgctgg aacgcttctg tcgcttcacg
    33301 caggttttgc ttaacaacgg gcaccgccgc cgcttctggc ggcggcaacg tcacctcagg
    33361 aaaagcaaaa tgttgcgcct caagaatcac ttcatcgccg ttgcgggtgg ctctcgccag
    33421 cactaccgcc cgatgaatag catgttccag ttcgcgcacg tttcccggaa aactgtagtg
    33481 ttgcagtaaa tttcgcgccc cggcacttaa taccacgcgg gagagcccct gccgcaaacg
    33541 acactgctcg cagaaatacc ccgccagcag aatgacatca tcgccccgct cacgcagcgg
    33601 cggcaccgaa agtggaaaca cgctcaggcg atgaaacaaa tcggcgcgga atcgccctgc
    33661 cagcacctct tcgcgtaagt cgcggttagt cgccgccagc acgcgcacat cgacccgcaa
    33721 actacggtca tcgccaacgc gctgaatatc gccatactgc aacaccctca gcagcttggc
    33781 ctgcaatgcc aacgacaact cgccgatctc atcgagaaac agagtaccgt tatccgccat
    33841 ttcaaacttc ccgctgcggt tactgatagc gccagtaaac gctcctttca catgcccgaa
    33901 caactcactt tccgccacac tttccggcag tgcggcacag ttgagataga ccagcggatt
    33961 caccgcccgt ggcgaggctt catgaatcgc tttcgccacc agctccttac cggttcccgt
    34021 ctcaccgctg atcagaacgt tgagatcgga cgccgccaca atctcaatct cttttttcag
    34081 ttgcgtcatg ccaggggaca agccaatcat ctgcgtctgt ttcaccgctt caaacggcgt
    34141 ggcatcgcct ggcagcatat tctggctttc cagttgttca atcagcaacg cattgcttaa
    34201 cgctcccgcc gccagcgcgg caatcagccg taactcttcg tcgctgaaaa catcgaactg
    34261 atcgggctgc atcccgtcga gcgtcagtgc gccgatcagg ttttgtccga caaacaatgg
    34321 cagaccaacg caggcgtgaa ccttcagact ctcctgccca ggaatcaaac cgtcataggg
    34381 atcgggcaat tcgctgtctg cgggaaagcg caccacatcc ccggcgcggg caatcgcttc
    34441 cagccgtgga tgcccttcca gcgcaaagcg tctaccgagt acatcctttg ccagaccgtc
    34501 gatggcaagc ggaataaact gccgcgaatc gtaacgtagc aacgcagacg catcgcactc
    34561 cagcacctga cgtagcgtgg tgatcaggcg ctgaaaacga tcctgatgac caatcccacg
    34621 ctgcaattcg atggcgatat tcgccagcac atcaacggaa aaactcatct ttgcctcact
    34681 gtcaatttga ctatagatat tgtcatatcg accatttgat tgatagtcat tttgactact
    34741 cattaatgag cataatttta tttatagagt aaaaacaatc agataagaaa ctggcacgca
    34801 atctgcaatt agcaagacat ctttttagaa cacgctgaat aaattgaggt tgctatgtct
    34861 attgtggtga aaaataacat tcattgggtg ggtcaacgtg actgggaagt gcgtgatttt
    34921 cacggcacgg aatataaaac gctgcgcggc agcagctaca acagctacct catccgcgaa
    34981 gaaaaaaacg tgctgatcga caccgtcgac cataaattca gccgcgaatt tgtgcagaac
    35041 ctgcgtaatg aaatcgatct ggcggatatc gattacatcg tgattaacca tgcagaagag
    35101 gaccacgccg gggcgctgac cgaactgatg gcgcaaattc ccgatacgcc gatctactgt
    35161 actgccaacg ctatcgactc gataaatggt catcaccatc atccggagtg gaattttaat
    35221 gtggtgaaaa ctggcgacac gctggatatc ggcaacggca aacagctcat ttttgtcgaa
    35281 acaccaatgc tgcactggcc ggacagcatg atgacttacc tgacaggcga cgcggtgctg
    35341 ttcagtaacg atgctttcgg tcagcactac tgcgacgagc atctgttcaa cgatgaagtg
    35401 gatcagacgg agcttttcga gcagtgccag cgttactacg ccaatatcct gacgccgttc
    35461 agccgcctgg taacgccgaa aattaccgag atcctgggct ttaacttacc agtcgatatg
    35521 atagcgacct cccacggcgt ggtctggcgc gataacccga cgcaaattgt cgagctgtac
    35581 ctgaagtggg ccgctgatta tcaggaagac agaatcacca ttttctacga caccatgtcg
    35641 aataacaccc gcatgatggc tgacgctatc gcccagggaa ttgcggaaac cgacccacgc
    35701 gtggcggtga aaattttcaa cgtcgcccga agcgataaaa acgaaatcct gaccaatgtc
    35761 ttccgctcaa aaggcgtgct ggtcggcact tcgacgatga ataacgtgat gatgccgaaa
    35821 atcgccgggc tggtggagga gatgaccgga ttacgcttcc gtaacaaacg cgccagtgct
    35881 ttcggatctc acggctggag cggcggtgcg atggatcgcc tttccacgcg cctgcaggat
    35941 gcgggtttcg aaatgtcgct cagcctgaaa gccaaatggc gaccagacca ggacgctctg
    36001 gagttatgcc gtgaacacgg tcgcgaaatc gcccgtcagt gggcgctcgc gccgctgccg
    36061 cagagcacgg tgaatacggt agttaaagaa gaaacctctg ccacgacgac ggctgacctc
    36121 ggcccacgaa tgcagtgcag cgtctgccag tggatttacg atccggcaaa aggcgagcca
    36181 atgcaggacg ttgcgccagg aacgccgtgg agtgaagtcc cggataactt cctctgcccg
    36241 gaatgctccc tcggcaaaga cgtctttgac gaactagcat cggaggcaaa atgagcaacg
    36301 gcattgtgat catcggttcg ggcttcgccg cccgccaact ggtgaaaaat attcgcaaac
    36361 aggacgccac tattccatta accctgattg ccgccgacag catagatgag tacaacaaac
    36421 ccgacctcag ccatgttatc agtcaggggc aacgtgccga cgaccttacc cgccagacgg
    36481 cgggtgaatt tgccgggcag tttaatctgc gcctgtttcc gcacacctgg gtgacggata
    36541 tcgatgccga agcccatgtg gtgaaaagcc agaataatca gtggcaatac gacaagttag
    36601 tgctggcaac cggtgccagc gcctttgtcc cgcctgtgcc tgggcgtgag ttaatgctga
    36661 cgttaaatag tcagcaagag tatcgcgcct gtgaaacgca actgcgggat gcccgacgcg
    36721 tgttgattgt cggcggtggt ttgattggta gcgaactggc gatggatttt tgtcgtgcag
    36781 gcaaagcggt cacgctaatc gacaacgctg ccagtattct ggcgtcatta atgccaccag
    36841 aagtaagcag ccgcttgcag catcggttga cggagatggg cgttcatctg ctgttgaaat
    36901 ctcagttgca ggggctggaa aaaacggatt ctggcattct ggcaacgctg gaccaccagc
    36961 gtagtatcga agtggatgcg gtaattgccg ccaccggact gcgcccggaa acagccctgg
    37021 cacgacgcgc cgggctgacg attaatcgcg gcgtttgcgt cgatagttat ctgcaaacca
    37081 gtaatgccga tatttacgcg ctgggcgatt gcgcggaaat taacggtcag gtattgccgt
    37141 tcctgcagcc gattcaactt agcgggatgg tgctggcaaa aaatcttctc ggcaataaca
    37201 cgccgctgaa actaccggcg atgctggtga aaatcaaaac gccggaatta ccgctgcatc
    37261 tggcaggcga aacccagcgt caggatttac gctggcagat taataccgaa cgccagggaa
    37321 tggtggcgcg cggcgttgac gatgctgacc agcttcgcgc ctttgtggtc agtgaggatc
    37381 ggatgaaaga ggcatttgta ttgttgaaaa cgttgccggt gtaggtgggc tactgtgcct
    37441 aaaatgtcgg atgcgacgct ggcgcgtctt atccgaccta cggggacgca tgtgtaggcc
    37501 ggataaggca tttacgccgc atccggcaag ggtgtccaaa tgcaacacgc tttatccgtt
    37561 ctggacttca cccgctaacc aacgcgccgc agcaataacc ccctgcccca gagacaaacc
    37621 gccatcaccc gccggtaaac tctgtggaaa gagcaatgtg aaatcagcga gataatgcgc
    37681 cagacgtgca cgcagcaaac ggttatgaat aaccccgccg ctaaatacca gcgtagtgat
    37741 accacgcatc gtggcctgct cacgcatcaa cgcggcaaaa ccctgcgcca gcgcatcatg
    37801 aaacgcccac gcgcgttgat taaccagtgc ctgccagttc agccactgct gccagaaagt
    37861 ggcgagatcc agttgattgt ccacccgcgg catcgtcacc ggatgcgtca ctccgtcgca
    37921 tgaggctgcg agcgcctcca gagcacaagc cgcttcacct tcataactta acgtggctgg
    37981 cgcacagccc agtgccgccg ccactgcatc gaacaaccgc ccacacgatg atgccagcgg
    38041 cgcgttaatt ccacgctcaa tggcccgcgc cagtacgctc cagttttgct gttgcacact
    38101 tgccgtttca gggtaattct gccactccgg cacaaagcgc aggcactgcg ccagcaggtt
    38161 tcgccacggc tgcttcgccg ccaaatcgcc acccggaagc gccactgcag gcaagccgcc
    38221 caggtgctca cattcgcgat agttcacccg caggcactcg ccgccccaca aagcgccatt
    38281 ctcccccata ccgataccgt cgagcgtcaa agcaatgaca tcaccgccat ccagcggcca
    38341 ctgatgctct gccagacacg ccgctgcatg ggcatgatga tgcagtaccg tttgcgtcgg
    38401 cagattcatt tcgctcgccc actggctgga gacatagccc ggatgcacat catgcacaat
    38461 gtattgcgga gtgaaatcgt agatgttttg catcaggcgt aacgcttcgc gccactgcat
    38521 ctggatgcca tcgtcactta aatcgcccag atgctgactc aacaccgctt gttcaccgcg
    38581 caccaggcag aaggtatttt tcagatccgc gccaagacac agcacaggcg gaacattttt
    38641 aaagcccgga ggcaaaacca gcgcatccgg cacatacccc cgcgaacggc gcagcatttc
    38701 gccgctttcg cgcaccactg aatcatccat ccgctgcacg atgtcgcggt tatgtagcaa
    38761 gaatccgtcg gcaatgccct gcaaatccgc cagcgcctgt tcgttgctga tagctggtgg
    38821 tttaccgctc aggttgccgg aggtcatcac cagcgggcat tgcagttcct gtaacagcaa
    38881 atgctggagc gggttcgcag gcaacatcac cccgacttcg ttaaggccag gggcgatatc
    38941 atcacaaagc tcaggtacgt attttttatc caccagcaca atcggcgcag cgggcgtggt
    39001 aagcaactgg cgcgcagcgt ctggtaaact ttctgccact ggcaacatga ctgccagcgg
    39061 tttcgccggg cgatgtttgc gctcccgcag tgtcgccacc gcgttactgt tacgtgcatc
    39121 gcaggcaaga tgaaatccgc caatcccttt gatggcgaca atgttgccca tttttaactg
    39181 tgcgatagct gcctgtaatg ccgcctcttg ttctgcatgt tcaccatgac ttacccattc
    39241 aagatgcggg ccacactccg ggcaggccac cggctgggcg tggaagcgac gatcgagcgg
    39301 gtcgcggtac tctttgtcac aggccggaca tagcggaaac gccgccatca cggtaaacgg
    39361 gcggtcgtaa ggcatcgcgc gaataatggt gaaacgcggg ccgcagtggg tacagttgat
    39421 aaacggataa cgataacgcc gctcgcctgg ggtattcatt tcggcaaggc aagcagggca
    39481 agtggcggca tccgggacaa tttgcgtatt catggtgccg ccggtgctct ggcggatggt
    39541 gaactcggtg ggcagttgtg accagataaa cggctcacgc tcgacgctat caatacgcgc
    39601 cagtggcgga cagtgctgat gcaattgaac aagaaacgtt tccgggtctt ccagcagccg
    39661 gacttctacg ccatcgccgt cattacatac atcgccgtga agatttaatt gctgtgccag
    39721 ctgccagaca aacggacgaa aaccgacgcc ctgcactttg ccacgaatac gcagttggac
    39781 accgcaagat gtgttttttg ccattgagtt attcccgcca tcatgaattg cgtaacccgc
    39841 cctgccggac acgacagcgt cgtatccggc agtctcaggt cggcgatacc gcccgctccg
    39901 tattctacga atatttccgg gaattccttt gatgccagaa cagttctgta agatttttag
    39961 aacatcagcg ccgtacggcg gcgtttttct gcgctcagtt gttcaagttt attacgatcg
    40021 acacaaatca gcgcatgggt cgggcaagcc gccatacacg ccgggccgtc ttcacgatgg
    40081 ttgcacaggt cgcatttatt ggcttcggct ttgtcagccc gtacattcag acccgcgccg
    40141 ctgttgcgaa tcacaggacg taccaccact tccatcgcgc catacgggca agccaccacg
    40201 caggttttgc aaccaatgca acgttcctgc atcacaggaa caaacccttt atcacggctg
    40261 atagcaccat tcgggcagac gttagcgcac ggtgcatctt cacactgacg gcaaactgtc
    40321 gccgtggaaa tgttcacacc tttaatgaca tggatacgcg gtaaaaatgt ttccggggtc
    40381 agcgatgcac agtcctgatt ttcctgatga gaaaccacgc acgccacttc acaggtacgg
    40441 caaccaatac atttactcgc gtcagcaatg atgaaacggt tcatcaaatt ctccagcaat
    40501 gacagttaat gcgccgatac actcacaaat catgccagtt tttaatttgc tgttatttaa
    40561 ggaaattaat ttctgtaatg caggaaaaaa gatgtcatcg acactagtga cgatgacatg
    40621 tgatgacaat gtttatcgcg aaagggcaat gagtgagtcg cggcggatca gttttccgct
    40681 aaaggttttc ggcggtgaga aatccccgcc atcgagcata aaaatcagcc gtccaataat
    40741 ttcctgaatc atctcagtca ccggaatttt tacgctggag agcgccggaa cgatataggg
    40801 ggcaattgcg atatcatcga atccgataac tgacacctgc tctggcaccg ctacgccgcg
    40861 ctcgtgcaac gctttgatcg cacctatcgc catatcgtcg ttactggcaa ctaacgcgct
    40921 aaatttagcc cctcgttcga gcaacgtttc taccccttcg gccccgctgg cgggcgtcca
    40981 tttaccgtta gcgataagtt tttcattgag cgcaatacca tgctgcgaca gcgcgtcttt
    41041 atacccggca agacgttcaa tgctggtggg ggaatccatc gagccggtaa ggaaagcaat
    41101 ctcctgatgc ccggcgttta tcaactctgc cacggcgtta aagctggtct gtttatgatc
    41161 gcaccagacg ctatggctgc tattattgcg caggcggcga ttaagcacca ttatcggctg
    41221 actgtgcgcg tcaatgatgt catcaatctc atccacgctt aaaaaacgcg ggtaaatcat
    41281 aatggcgtcg cagcgcaaat ccagcagata ctgaatcgcc tggcgctctt cttcggcgct
    41341 gtgtttacca tctgccaata gcaactgtcg ccctttctct tccgccattc gcgcggcatg
    41401 aaacagtaat tcactaaaat aaatgccgtg ataaagcgtg ttggtcacta ccagccccag
    41461 cgtctgagta ctcttcgccg acagattgcg ggccagcaaa tttgggcggt aactgctctc
    41521 ttctaccgcc tgaaacacgc gatctttagt ctcctggctg acgtagccat tacctgaaag
    41581 cacgcgggaa acggtcgctt ttgaaacccc ggcgcgcttc gccacttcca gcatcgtcgt
    41641 catcattttc atccctttac acgcaatcaa cgcagtgtac tgcaccgttt gccgattgtc
    41701 cttgcgcaat cggctccagc ggataagacg aggtcgccgt gccggttttg atgacttttt
    41761 tgataaaggt aaacatggcg attccttatt tcagcggcga gtttttacgc tcaatgctgt
    41821 aacgctcgag ttctttgtac ggcaccactt tgctcttctt cttacgcaca tcgactacgg
    41881 tcatgcggtc ggtacaggag tagcaagggt ccaggctacc gataatcagc ggcgcatagg
    41941 aaacggtatt gccacgcagc atgtagcgca gggtcggcca gttcgcgtag gtcgcggcac
    42001 ggcagcgcca gcggtacagc ttctggttgt cgccggtcat gctccagtgg atatcgtcgc
    42061 cgcgcggcgc ttcggcaaag cccagcgcga aacggtgcgg aatgtaggta aagccttcca
    42121 ccatcagcgg accgcccggc aggttatcca gaccgtagtc gatcatattc agcgcggtat
    42181 agacctcgtt gattcgcact ttcagacggg aaataacgtc gcagccctgc tcgctgtgga
    42241 cttccattgg cagcaggcca taaccgacaa acgggtgatc ggcgcgagta tcgcgggcga
    42301 gaccgctggc gcgaaccatc gggccaacgt tactgaaatc gcgggcgatt tccgggtcca
    42361 gacgaccaat gccgacggtg cgttgttcca tgttcggcgt actcagcagc acatccacca
    42421 gctcctgcac ttcacgacgc atttgttgcg ccagctggcg ggtctggatc atgtcatctt
    42481 tcagcagatc gcgacgaatc ccgccgatca ggttcaggcc gtaggtttta cgcgcaccgg
    42541 taaggatctc tgccattttc atggaggttt cacgcacgcg gaagaactgc ataaagccgg
    42601 agtcgaagcc ggtaaagtga caggcgaggc cgaggttgag cagatgcggg tgcaggcgtt
    42661 ccacctccag cagaatggcg cggatcatct gcgcgcgttc tggcacctga atacccatcg
    42721 cgttttccac cgacgtggtg taggcggtgc tgtgggcaaa gccgcagatc ccgcacacac
    42781 ggtcagagag gaaggtcact tcgttataac ccatgcgggt ttccgccagt ttttccatac
    42841 cgcgatggac gtagaacaga cggtagtcgg cgtcgataat gttttcgcca tcgacgaaca
    42901 gacggaagtg acccggttcg tcagaagtga cgtgcagcgg accaatcggc acgacgttgt
    42961 ttttcttgtc gcccagttcg ttgatgaact cgtaggtttc agcatcggta gtcggtgccg
    43021 gacgctgacg ataatccatg ctgtctttac gcagcggata aagttcatcc ggccagtcat
    43081 ccggcagcac cagacgacgt tcatccggca gaccaaccgg aatcaaaccg tacatatcgc
    43141 gcacttcgcg ctcgccccac accgccgccg gaacgcgcgg cgtcacggac ggatactccg
    43201 gtttgttggc gtcaacttcg acgcgaaccg ttacccaaca cttggtgccc ttctccatcg
    43261 acagcacgta ataaacggcg taatgaccat tcagtttgcg ttcgtcgtta ccaaacagca
    43321 ccgacagcca gccaccctgc ttgtagtaga gaaactccac cacttccggc agatagttca
    43381 cctttacggt gacagtcagc tgatctttgg tctgccaggc gtggtccagc acgacgccta
    43441 gaaatgcctc attcagcgcg gcgagataat gttgacctaa tttttcttca gacatgctca
    43501 aactctcttt aatcacgccg ccagcaagga gacgaacgct aaaaatgcaa agccaaaccc
    43561 agcccaggta atgcgcggag taatatcaag acgcagacgg gccatgctgt tttcgaacag
    43621 cgcgataacc aggacgccga ccaccagttt gacgatggca atcaccagcg ccagcagcag
    43681 tccaccgacg gtgaaggttt ccatttgtcc ccacggaata aacaccccga cgaacatctg
    43741 caacaccacc agctgtttca ggctgatacc ccatttcatg acgccaaagc cgctaccgct
    43801 gtattcggag agcggacctt cctgcaactc ctgctcggct tccgccaggt cgaacggcag
    43861 tttgcccatt tcgataaaag tggcgaacgc acaggcgcag agcgccagca ccagcgggat
    43921 gctttggctc agcggccagt gataaacggt gtcggtaatg ttgctgatgt tggtggaacc
    43981 ggcaacctgt gcggcaaccc acagaccaag cagcaacatc ggttcgacca gcacgccaag
    44041 catcgcttca cggctcgcac cgatagcggt aaacgggcta ccggtatcca gaccggaaat
    44101 ggcaaagaag aaacgcgcga tggcgaagag atacagtaag gtgatcaaat cgcccagttg
    44161 cggcagcgga gaaccgacgg tcaccaccgg cagcgcagta gcgatagtca gcatgacgcc
    44221 caccatcaca tacggcgtca ggcggaacat ccagccagag gcatccgggc caacgctctg
    44281 gcggcccagc agtttgataa tgtcgcgata ctcctgcaac acgcccggcc cgcgacggtt
    44341 atgcagacgg gcgcgcgcca cgcgggtaat accggagagc agcggcgcaa cggcaaataa
    44401 caccagcgcc tgaattaacg gatataaaac actcattctc aggctcctcg tgaaacaata
    44461 atcaccacca gtaccgccag ttcaaccagc gccatccggc ggaacagcaa cgcactacct
    44521 tcgcactgcc agcccggcac tagcgacacc ggattcagcc atttgcgcag tttcagcact
    44581 ggcgcaaacg cctgtttaac cggcatggca aaaccgtgag cggtaatcac cattgatttt
    44641 tcatggtcgt agccgcacac ccaggccgca ccgcgggaac gtgacggcag gcggtcgcct
    44701 ttgcaaatcg ccataatgat gaatggcagc agcgggcagg caatcagcag caatgtgatc
    44761 attggttgag aaacggtggt gttagcaggc tccagaggca gaggtacagc agcagagagc
    44821 atcggcagca gccacggcgc agcaacaccg ccaattacgc agcaaatcgc cagtgccact
    44881 acgcttacgc tcatcaggag cggcgcacag gtggcgtttt cggcttcttt ggtacgcggc
    44941 gcgccgagga acgtgacgcc atagactttc gccatacaca tcaccgccag cgcaccggta
    45001 attgccagac ccacagcgag cagcggcccc agcagacggg caacaaacgc gccgctattg
    45061 ctcagtttga agaaggattg atagataacc cattccccgg caaacccgtt cagtggcggc
    45121 agcgcagcca ttgccatcaa cccgactaac atggcgatgg agataaccgg cattttcttg
    45181 ccaataccac cgagtttttc gatatcgcga tgaccggtac ggaaccagac gctccccgcc
    45241 ccgaggaaca gtacgctttt gaacaggcta tggttaagca gatggtacag accaccgacc
    45301 aggccaagag caatcagcgc cggttgttcg agcgcgatac ccgttacgcc agcgcccagc
    45361 cccagcccca gccccagccc cagccccagc cccagcccca gccccagccc cagccccagc
    45421 cccagcccca gcaggataat gccgatattt tccagggtgt ggtaagccag caggcgctgg
    45481 atgttgtgtt ccatcaacgc atacagacca ccgacgaacg cggtgatcat gccgagcacc
    45541 agcagcgcga tcccccacca cagcggtgca ttaccgccca gcagtgacag ggttaacatg
    45601 cccagcaggc caattttcat gactaccgta gaaaacaacg cggcagctgg cgcagaggcg
    45661 ttagcatgtg cctgcggcac ccagccgtgc agcggaataa tcccagccag caggccaaag
    45721 ccaatcacac cgagcagcca gatatcggaa ccgagcggca gctgttgcat acgcatatcc
    45781 agcaggcgca gatccagcgt gccataacgc tgccacagca gccagcaagc aatcaccagc
    45841 agcagagtgc caagacgcct cagcgcaaac cacagtttgc cctctttgct gttgctggtg
    45901 aggaacaccg cacacaaggc catgacttcg gccattacca cgaacatgcc gaggttgctg
    45961 gcgatgacgg cgcagacggc ggcagccatc aacatattga tctgcaagcc gttgcacttc
    46021 acctgcgcgt ggcgatgcca gtcaatgtta tagaggctga caaacagacc gcaaaggccg
    46081 agggtaatca gccaaatcgc gttaagcggg gagatttgca catcgtagct taccagcgac
    46141 agcgcaccgc tcacgccaac cgcgccagtc agtacagtga agcccgcggc tgccgtatac
    46201 agactaccaa ccgcgccgcc aattccggct atccagccac ttagcgcttt ttgaaaagag
    46261 aaatgccaga acagcggcgg cgacaaacca tgccacgccg ctattgatca gggaaattgc
    46321 gctcatttag cctctccact ttgagcctgc tgaaacaagg tgagatcgcc aaagtccgtg
    46381 ttaaaggtca gctcacgctt acgtttgctg acgcgggcga tatcggtgtt atccaccaga
    46441 tgcagggctt tagtcgggca catccgcaca caggccggac cttgttcatc aaagctacaa
    46501 aggtcacatt tgacggcgat cgcgcgaata cctggcaccc agtcaagcaa tgtgctgaca
    46561 cgcgccggag caggcggtgc cggtggcgct ttcggggtat tggcgtttgc cggaatatcc
    46621 agcggacggc tgccggaaaa ttcaattgcg ccaaacgggc aggcgatgcc gcacagtttg
    46681 cagcttacgc acagactttc attcaactgc acggccccat caacgcgggt gatggcgtta
    46741 accgggcaga ccaccgcgca gggtgcatct tcacagtggt gacagagctg cggcgcagat
    46801 tctttttcat tgagcatcac tctcaggcgc ggcattgatt gcaggccgtg ctggcgatgc
    46861 gtctctgaac aggcggcctc acaggtgtgg cagccgatac agagcgtgga gtcggcaatt
    46921 acaaaacgat tcaccaggca ttcctcaggt gattgtcatt tttgacgaaa acatgccgat
    46981 gaaatgtcat tttcgacact catcgacacg cccatcccca aacaggcgta acgcctgcaa
    47041 aacgggcaaa gcctcagctc atgctgccgg gctttgtccc tttaccagtt ggcttaaatt
    47101 caccggcaca ttgttttcaa tggcggtgta taagctgtcg taaacgccag cgattttttc
    47161 gagatagtgt tccagaacct gttcgcgatc gcggatgttg accttgccat caatcatgcc
    47221 gtcaatgctc agttgcgcct gcgcaatccg ctgtttgtct tcgccatctt ttgtttcgac
    47281 gtaatactcg atggtgtggc tgttaaagcc atccgccagg gtgacgtaaa tacgaaaatg
    47341 ttcaaaaagg acgaaacaga gttctttgtc cggcgcgcag ctcatggcgt gatgcaggcg
    47401 cgctttcgat aacgtgatcc cctgaaccag cgaattgcag tagatgtgcc actggtcctg
    47461 taggcgacga tgccgctgtg cgatgtaatc ggctttctcg cttatttccc aaatagtcat
    47521 tgtcaggtta ctcgtttaac agagatgcca gctttaagca ttttttgtgc caacttttaa
    47581 tttattgtta ttaaagagat ttttaagcta aagatgaatt tcgtcgccgt gtcgacgtgt
    47641 catttcgaca tcatcgacat tattcaccgc agggataatc aacactggcg caattattgc
    47701 ttgtagctgg caatagttaa tgggaggcga tatgcacgaa ataaccctct gccaacgggc
    47761 actggaattg atcgaacagc aggccgcaaa acacggcgca aaacgcgtaa ctggggtctg
    47821 gctcaaaatt ggcgcatttt cttgtgtcga aaccagctct cttgcctttt gttttgatct
    47881 ggtttgccgc ggcagcgtgg cggaaggttg taaactgcac ctcgaagaac aagaggccga
    47941 atgctggtgt gaaacatgcc aacagtatgt gacgctactg acccagcgcg tccgccgctg
    48001 tccacagtgt catggtgaca tgctacagat tgtggcagac gacggtttac agattcggcg
    48061 gatagaaata gaccaggagt gagcgatgtg tacaacatgc ggttgcggtg aaggcaacct
    48121 gtatatcgag ggtgatgaac ataaccctca ttccgcgttt cgtagcgcgc catttgcccc
    48181 ggcggcacgc ccgcagatga aaatcaccgg cattaaagcg cctgaattta cccccagcca
    48241 gactgaagaa ggcgacctgc attacggtca tggcgaagcg ggcactcaca caccgggcat
    48301 gagccagcgt cggatgctgg aagtcgaaat tgacgtgctg gacaaaaaca accgtctggc
    48361 tgaacgcaac cgcgcgcgct ttgctgcccg caagcaactg gtgctcaacc tggtttccag
    48421 ccctggttcc ggtaaaacca ccctgctgac ggaaacctta atgcgcctga aagacagcgt
    48481 tccgtgcgcg gttattgaag gcgaccagca aaccgtgaac gatgccgcac gcattcgcgc
    48541 taccggtaca ccagcgattc aggtgaacac cggtaaaggc tgccatcttg acgcacagat
    48601 gattgccgac gccgcaccgc gtctgccact ggacgataac ggtattctgt ttatcgaaaa
    48661 cgttggcaac ctcgtatgcc cggccagctt cgatctcggt gaaaaacaca aagtagcggt
    48721 gctttccgtt accgaaggtg aagacaaacc gctgaaatat ccgcatatgt ttgccgccgc
    48781 ctcgctgatg ctgctcaaca aagttgacct gttgccgtat ctcaactttg acgttgagaa
    48841 gtgcatcgcc tgcgcccgcg aagtcaatcc agaaattgaa atcatcctta tttccgccac
    48901 cagcggcgaa gggatggacc agtggctgaa ctggctggag acacagcgat gtgcataggc
    48961 gttcccggcc agatccgcac cattgacggc aaccaggcga aagtcgacgt ctgcggcatt
    49021 cagcgcgatg tcgatttaac gttagtcggc agctgcgatg aaaacggtca gccgcgcgtg
    49081 ggccagtggg tactggttca cgttggcttt gccatgagcg taattaatga agccgaagca
    49141 cgcgacactc tcgacgcctt acaaaacatg tttgacgttg agccggatgt cggcgcgctg
    49201 ttgtatggcg aggaaaaata atgcgttttg ttgatgaata tcgcgcgccg gaacaggtga
    49261 tgcagttaat tgagcatctg cgcgaacgtg cttcacatct ctcttacacc gccgaacgcc
    49321 ctctgcggat tatggaagtg tgtggcggtc atacccacgc catttttaaa ttcggcctcg
    49381 accagttact gcctgaaaac gttgagttta tccacggtcc gggttgcccg gtgtgcgtac
    49441 tgccgatggg tagaatcgac acctgcgtgg agattgccag ccatccggaa gtcatcttct
    49501 gtacctttgg cgacgccatg cgcgtaccag ggaaacaggg atcgctgttg caggcaaaag
    49561 cacgcggtgc cgatgtgcgc atcgtttact cgccgatgga tgcgttgaaa ctggcgcagg
    49621 agaatccaac ccgcaaagtg gtgttcttcg gcttaggttt tgaaaccacc atgccgacca
    49681 ccgccatcac tctgcaacag gcgaaagcgc gtgatgtgca gaatttttac ttcttctgcc
    49741 agcacattac gcttatcccg acgctgcgca gtttgctgga acagccggat aacggtatcg
    49801 acgcgttcct cgcgccgggc cacgttagta tggttatcgg cactgatgcc tataatttta
    49861 tcgccagcga ttttcatcgt ccgctggtgg tcgctggttt cgaacccctt gatctactgc
    49921 aaggcgtggt catgctggtg gagcagaaaa tagcggccca cagcaaggta gagaatcagt
    49981 atcgtcgggt ggtaccggat gccggtaacc tactggcgca acaggcgatt gccgatgtgt
    50041 tctgtgtcaa cggcgacagc gaatggcgcg gcttaggcgt gattgaatct tctggcgtgc
    50101 acctgacgcc ggattatcaa cgattcgatg ccgaagcaca tttccgcccg gcaccgcagc
    50161 aggtctgcga tgacccgcgc gcgcgttgtg gtgaggtatt aacgggcaaa tgtaagccgc
    50221 atcaatgccc gctgtttggt aacacctgta atcctcaaac cgcatttggt gcactgatgg
    50281 tctcctccga aggagcgtgc gccgcgtggt atcagtatcg tcagcaggag agtgaagcgt
    50341 gaataatatc caactcgccc acggcagcgg cggccaggcg atgcagcaat taatcaacag
    50401 cctgtttatg gaagcctttg ccaacccgtg gctggcagag caggaagatc aggcacgtct
    50461 tgatctggcg cagctggtag cggaaggcga ccgtctggcg ttctccaccg acagttacgt
    50521 cattgacccg ctgttctttc ctggcggtaa tatcggcaag ctggcgattt gcggcaccgc
    50581 gaatgacgtt gcgggcagtg gcgctattcc gcgctatctc tcctgtggct ttatcctcga
    50641 agaaggattg ccgatggaga cactgaaagc cgtagtgacc agcatggcag aaaccgcccg
    50701 cacggcaggc attgccatcg ttactggcga tactaaagtg gtgcagcgcg gcgcggcaga
    50761 taaactgttt atcaacaccg ctggcatggg cgcaattccg gcgaatattc actggggcgc
    50821 acagacgcta accgcaggcg atgtattgct ggttagcggt acactcggcg accacggggc
    50881 gactatcctt aacctgcgtg agcagctggg gctggatggc gaactggtca gcgactgcgc
    50941 ggtgctgacg ccgcttattc agacgctgcg tgacattccc ggcgtgaaag cgctgcgtga
    51001 tgccacccgt ggtggtgtaa acgcggtggt tcatgagttc gcggcagcct gcggttgtgg
    51061 tattgaaatt tctgaatcag cgctgccggt taaacctgcc gtgcgcggcg tttgcgaatt
    51121 gctgggactg gacgccctga actttgccaa cgaaggcaaa ctggtgatcg ccgttgaacg
    51181 caacgcggca gagcaagtgc tggcagcgtt acattcccat ccactgggga aagacgcggc
    51241 gctgattggt gaagtggtgg aacgtaaagg tgttcgtctt gccggtctgt atggcgtgaa
    51301 acgaaccctc gatttaccac acgccgaacc gcttccgcgt atatgctaat aaaattctaa
    51361 atctcctata gttagtcaat gaccttttgc accgctttgc ggtgctttcc tggaagaaca
    51421 aaatgtcata tacaccgatg agtgatctcg gacagcaagg gttgttcgac atcactcgga
    51481 cactattgca gcagcccgat ctggcctcgc tgtgtgaggc tctttcgcaa ctggtaaagc
    51541 gttctgcgct cgccgacaac gcggctattg tgttgtggca agcgcagact caacgtgcgt
    51601 cttattacgc gtcgcgtgaa aaagacaccc ccattaaata tgaagacgaa actgttctgg
    51661 cacacggtcc ggtacgcagc attttgtcgc gccctgatac gctgcattgc agttacgaag
    51721 aattttgtga aacctggccg cagctggccg caggtgggct ataccccaaa tttggtcact
    51781 attgcctgat gccactggcg gcgggagggc atatttttgg tggctgtgaa tttattcgtt
    51841 atgacgagcg tccctggagc gaaaaagagt tcaatcgtct gcaaacattt acgcagatcg
    51901 tttctgtcgt caccgaacaa atccagagcc gcgtcgtcaa caatgtcgac tatgagttgt
    51961 tatgccggga gcgcgataac ttccgcatcc tggttgccat taccaacgcg gtgctttccc
    52021 gcctggatat ggacgaactg gtcagcgaag tcgccaaaga aatccattac tatttcgaca
    52081 ttgacgatat cagcattgtc ttacgcagcc accgtaaaaa caaactcaac atctactcca
    52141 ctcactatct tgataaacaa catcccgccc acgaacagag cgaagtcgat gaagccggaa
    52201 ccctcaccga acgcgtgttc aaaagtaaag agatgctgct gatcaatctc tacgagcggg
    52261 acgatttagc cccctatgaa cgcatgttgt tcgacacctg gggcaaccag attcaaacct
    52321 tgtgcctgtt accgctgatg tctggcgaca ccatgctggg cgtgctgaaa ctggcgcaat
    52381 gcgaagagaa agtgtttacc actaccaatc tgaatttgct gcgccagatt gccgaacgtg
    52441 tggcaatcgc tgtcgataac gccctcgcct atcaggaaat ccatcgtctg aaagaacggc
    52501 tggttgatga aaacctcgcc ctgaccgagc agctcaacaa tgttgatagt gaatttggcg
    52561 agattattgg ccgcagcgaa gccatgtaca gcgtgcttaa acaagttgaa atggtggcgc
    52621 aaagtgacag taccgtgctg atcctcggtg aaactggctc gggtaaagag ctgattgccc
    52681 gtgcgatcca taatctcagt gggcgtaata atcgccgcat ggtcaaaatg aactgcgcgg
    52741 cgatgcctgc cggattgctg gaaagcgatc tgtttggtca tgagcgtggg gcttttaccg
    52801 gtgccaacgc ccagcgcatt ggtcgttttg aactggcgga taaaagctct ctgttcctcg
    52861 acgaagtggg cgatatgcca ctggagttac agccgaagtt gctgcgtgta ttgcaggaac
    52921 aggagtttga acgtctcggc agcaacaaaa tcattcagac ggacgtacgt ttaattgccg
    52981 cgactaaccg cgatctgaaa aaaatggtcg ccgaccgaga gttccgtagc gatctctatt
    53041 accgcctgaa cgtattcccg attcacctgc cgccactacg cgagcgtccg gaagatattc
    53101 cgctgctggc gaaagccttt accttcaaaa ttgcccgtcg tctggggcgc aatatcgaca
    53161 gcattcctgc cgagacgctg cgcaccttga gcaacatgga gtggccgggt aacgtacgcg
    53221 aactggaaaa cgtcattgag cgcgcagtat tgctaacacg cggcaacgtg ctgcagctgt
    53281 cattgccaga tattgcttta ccggagcctg aaacgccgcc tgccgcaacg gttgtcgccc
    53341 aggagggcga agatgaatat cagttgattg tgcgcgtgct gaaagaaacc aacagcgtgg
    53401 ttgccgggcc taaaggcgct gcgcaacgtc tggggctgaa acgcacgacc ctgctgtcac
    53461 ggatgaagcg gctgggaatt gataaatcgg cattgattta actgcaaatt gccggacaga
    53521 tctgcctgtc cggcatacta ttcacgaggt tttttcggac gatatttttc cggcagttct
    53581 ggcaccggac gcttgtcatc gatgagatga cgcacggtta agatcggatg acgccacagc
    53641 attctcggcc cggcccaacg cataatctgt ttcatctctt cacgctttgc aggctggtaa
    53701 cagtgcaccg gacactgctt acaggctggt ttctcttcgc cgaacacaca tttatccagc
    53761 cgcttttgcg cgtaaacaaa caacgcctcg taatgctccg gctccgctga cgcctgcggg
    53821 catttcgctt gataaagatc gatcattttt ttaatcgtca gtttttcacg agagatacgc
    53881 ttgccggaca tgctgcctcc acctcattaa gatgcattta tattacatct taat
//
LOCUS       DAJTRD010000019        53212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTRD010000019 DAJTRD010000000
VERSION     DAJTRD010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.019"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..274)
                     /locus_tag="OO315_001230"
     CDS             complement(<1..274)
                     /locus_tag="OO315_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5890216.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
     gene            complement(287..2068)
                     /gene="gcl"
                     /locus_tag="OO315_001231"
     CDS             complement(287..2068)
                     /gene="gcl"
                     /locus_tag="OO315_001231"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5890217.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(2158..2973)
                     /gene="allR"
                     /locus_tag="OO315_001232"
     CDS             complement(2158..2973)
                     /gene="allR"
                     /locus_tag="OO315_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5890218.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(3051..3533)
                     /gene="allA"
                     /locus_tag="OO315_001233"
     CDS             complement(3051..3533)
                     /gene="allA"
                     /locus_tag="OO315_001233"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5890219.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            3763..4689
                     /gene="allS"
                     /locus_tag="OO315_001234"
     CDS             3763..4689
                     /gene="allS"
                     /locus_tag="OO315_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5890220.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            4758..5852
                     /gene="mnmH"
                     /locus_tag="OO315_001235"
     CDS             4758..5852
                     /gene="mnmH"
                     /locus_tag="OO315_001235"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5890221.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(6565..8980)
                     /locus_tag="OO315_001236"
                     /pseudo
     CDS             complement(6565..8980)
                     /locus_tag="OO315_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(8977..9663)
                     /locus_tag="OO315_001237"
     CDS             complement(8977..9663)
                     /locus_tag="OO315_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5890222.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            9634..10256
                     /gene="tesA"
                     /locus_tag="OO315_001238"
                     /pseudo
     CDS             9634..10256
                     /gene="tesA"
                     /locus_tag="OO315_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            10246..11055
                     /gene="ybbO"
                     /locus_tag="OO315_001239"
     CDS             10246..11055
                     /gene="ybbO"
                     /locus_tag="OO315_001239"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5890223.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            11116..11970
                     /gene="cnoX"
                     /locus_tag="OO315_001240"
     CDS             11116..11970
                     /gene="cnoX"
                     /locus_tag="OO315_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5890224.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(12033..12812)
                     /gene="fetB"
                     /locus_tag="OO315_001241"
     CDS             complement(12033..12812)
                     /gene="fetB"
                     /locus_tag="OO315_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5890225.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(12799..13476)
                     /gene="fetA"
                     /locus_tag="OO315_001242"
     CDS             complement(12799..13476)
                     /gene="fetA"
                     /locus_tag="OO315_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5890226.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            13622..14539
                     /locus_tag="OO315_001243"
     CDS             13622..14539
                     /locus_tag="OO315_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5890227.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            14536..14994
                     /locus_tag="OO315_001244"
     CDS             14536..14994
                     /locus_tag="OO315_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5890228.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(14995..15402)
                     /gene="cueR"
                     /locus_tag="OO315_001245"
     CDS             complement(14995..15402)
                     /gene="cueR"
                     /locus_tag="OO315_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5890229.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(15527..16819)
                     /locus_tag="OO315_001246"
     CDS             complement(15527..16819)
                     /locus_tag="OO315_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5890230.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            complement(16822..17754)
                     /gene="glsA"
                     /locus_tag="OO315_001247"
     CDS             complement(16822..17754)
                     /gene="glsA"
                     /locus_tag="OO315_001247"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5890231.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            18016..20520
                     /gene="copA"
                     /locus_tag="OO315_001248"
     CDS             18016..20520
                     /gene="copA"
                     /locus_tag="OO315_001248"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5890232.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            complement(20735..21076)
                     /locus_tag="OO315_001249"
     CDS             complement(20735..21076)
                     /locus_tag="OO315_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5890233.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            21214..22008
                     /locus_tag="OO315_001250"
     CDS             21214..22008
                     /locus_tag="OO315_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5890234.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            22212..22691
                     /gene="ybaK"
                     /locus_tag="OO315_001251"
     CDS             22212..22691
                     /gene="ybaK"
                     /locus_tag="OO315_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5890235.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(22728..24380)
                     /gene="ushA"
                     /locus_tag="OO315_001252"
     CDS             complement(22728..24380)
                     /gene="ushA"
                     /locus_tag="OO315_001252"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5890236.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            24598..25818
                     /gene="fsr"
                     /locus_tag="OO315_001253"
     CDS             24598..25818
                     /gene="fsr"
                     /locus_tag="OO315_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5890237.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            26056..27732
                     /locus_tag="OO315_001254"
     CDS             26056..27732
                     /locus_tag="OO315_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5890238.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27865..29169)
                     /gene="gsk"
                     /locus_tag="OO315_001255"
     CDS             complement(27865..29169)
                     /gene="gsk"
                     /locus_tag="OO315_001255"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5890239.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            29321..30280
                     /gene="aes"
                     /locus_tag="OO315_001256"
                     /pseudo
     CDS             29321..30280
                     /gene="aes"
                     /locus_tag="OO315_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            complement(30277..31239)
                     /gene="hemH"
                     /locus_tag="OO315_001257"
     CDS             complement(30277..31239)
                     /gene="hemH"
                     /locus_tag="OO315_001257"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5890240.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(31371..32015)
                     /gene="adk"
                     /locus_tag="OO315_001258"
     CDS             complement(31371..32015)
                     /gene="adk"
                     /locus_tag="OO315_001258"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5890241.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            complement(32196..34070)
                     /gene="htpG"
                     /locus_tag="OO315_001259"
     CDS             complement(32196..34070)
                     /gene="htpG"
                     /locus_tag="OO315_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5890242.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            complement(34180..34785)
                     /gene="recR"
                     /locus_tag="OO315_001260"
     CDS             complement(34180..34785)
                     /gene="recR"
                     /locus_tag="OO315_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5890243.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(34785..35114)
                     /locus_tag="OO315_001261"
     CDS             complement(34785..35114)
                     /locus_tag="OO315_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5890244.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(35167..37092)
                     /gene="dnaX"
                     /locus_tag="OO315_001262"
     CDS             complement(35167..37092)
                     /gene="dnaX"
                     /locus_tag="OO315_001262"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5890245.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    complement(35770..35834)
                     /gene="dnaX"
                     /locus_tag="OO315_001262"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(37221..37772)
                     /gene="apt"
                     /locus_tag="OO315_001263"
     CDS             complement(37221..37772)
                     /gene="apt"
                     /locus_tag="OO315_001263"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5890246.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(37925..38302)
                     /locus_tag="OO315_001264"
     CDS             complement(37925..38302)
                     /locus_tag="OO315_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5890247.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            38372..38899
                     /gene="priC"
                     /locus_tag="OO315_001265"
     CDS             38372..38899
                     /gene="priC"
                     /locus_tag="OO315_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5890248.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            38913..39074
                     /gene="rsmS"
                     /locus_tag="OO315_001266"
     CDS             38913..39074
                     /gene="rsmS"
                     /locus_tag="OO315_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5890249.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(39286..42648)
                     /gene="mscK"
                     /locus_tag="OO315_001267"
     CDS             complement(39286..42648)
                     /gene="mscK"
                     /locus_tag="OO315_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5890250.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(42776..43423)
                     /gene="acrR"
                     /locus_tag="OO315_001268"
     CDS             complement(42776..43423)
                     /gene="acrR"
                     /locus_tag="OO315_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5890251.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            43565..44758
                     /gene="acrA"
                     /locus_tag="OO315_001269"
     CDS             43565..44758
                     /gene="acrA"
                     /locus_tag="OO315_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5890252.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            44781..47930
                     /locus_tag="OO315_001270"
     CDS             44781..47930
                     /locus_tag="OO315_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5890253.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            48476..48850
                     /gene="tomB"
                     /locus_tag="OO315_001271"
     CDS             48476..48850
                     /gene="tomB"
                     /locus_tag="OO315_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5890254.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            48876..49094
                     /gene="hha"
                     /locus_tag="OO315_001272"
     CDS             48876..49094
                     /gene="hha"
                     /locus_tag="OO315_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5890255.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            49266..49817
                     /gene="maa"
                     /locus_tag="OO315_001273"
                     /pseudo
     CDS             49266..49817
                     /gene="maa"
                     /locus_tag="OO315_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            49933..50403
                     /locus_tag="OO315_001274"
     CDS             49933..50403
                     /locus_tag="OO315_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5890256.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            50567..52117
                     /gene="pdeB"
                     /locus_tag="OO315_001275"
     CDS             50567..52117
                     /gene="pdeB"
                     /locus_tag="OO315_001275"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5890257.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(52159..52512)
                     /locus_tag="OO315_001276"
     CDS             complement(52159..52512)
                     /locus_tag="OO315_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5890258.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(52632..52728)
                     /gene="ffs"
                     /locus_tag="OO315_001277"
     ncRNA           complement(52632..52728)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO315_001277"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            52891..53202
                     /locus_tag="OO315_001278"
     CDS             52891..53202
                     /locus_tag="OO315_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5890259.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
BASE COUNT        12675 a        14016 c        13689 g        12832 t
ORIGIN      
        1 gaatcgctgc tgctacgcca tcccgaaact cttcttcacg gccaggaata caggcaatac
       61 cgcgctcgcc cgccgcccag tcacctgccg gtaaattgtg cagcgtatgt tcgagtttat
      121 tactcgccag tacctgtttt aattcttcaa tgtcgtagtc ataaggaaac ataaattcaa
      181 cgccgcgaaa accacactgc gcagctttct caaaacgggc gagaaaatca tattcaccaa
      241 ataacatcga taaattagca gagaaacgta acatcattta tctcccttat tcatagtgca
      301 tgaagcaggt ttcagtcggt gcatccgctg cgttatcggc gatatcttca aattccatga
      361 cgttatccag ttcgctgccc atcgaaatat tggtcacacg ctcgagaata acttccacga
      421 ctaccggtac ccgatattgc gccattaagg ctttcgcctg ttcaaaggct ggcgcaatat
      481 cttccggttt gaagacccga atagctttac aacctaaacc ttccgctact tttacgtggt
      541 caacaccgta gccattcact tcactggagt tgatattctc gaaagcgagt tgcacgcagt
      601 agtccatgtc aaaagcgcgt tgtgactgac gaatcaggcc gagataggcg ttattcacca
      661 gcacatggat gtacggaatg ttgaactgcg cgccaacggc taactcttca atcaggaact
      721 ggaagtcaaa gtcgccagaa atcgccacca cattgcgttt cggatcagcg gcacaacccc
      781 ccagcgccgc cggaatcgtc cagcctaacg gaccagcctg accacagttg atccagtggc
      841 ggtctttaaa gacatgcagc atttgtgccg cagcgatttg tgacagacca atagtggtga
      901 cataacaaac atcgcgacca aaggctttgt tcatctcttc atacacgcgc tgcggtttca
      961 ccggcacgtt gtcgaagtgg gttttgcgca gcaaagtgcg tttacgctgc tggcagtcgg
     1021 cgacccattc tttacgacac ggcagacgac ccgctttttg catctcctgc gccacttcaa
     1081 ccagcagtgt cagcgccgct ttagcatcag agacaatgcc gagatccgga cacagcacgc
     1141 gaccaatttg cgtcggctca atatcaatat gaacgatttt gcgcccttcg gtgtatttct
     1201 ctaccgaacc ggtatgacgg ttagcaaaac ggttaccgat accaaacacc atgtcggacg
     1261 ccagcaacgt tgcgttaccg taacgatgcg cggtttgcag accgaccatc ccggccatca
     1321 gttcatgatc gtccgggata cagccccagc ccatcagcgt tgggatcacc ggaacgctgg
     1381 tcagttcagc aaactgttgt aacagtgcag ctgcgtcagc attgattacc ccgcccccgg
     1441 caacaatcac cggacgttcg gcctggatta acatttctac agctttttcg atctgcatac
     1501 ggctggcagc aggtttgtag accggcagcg gttcgtacat gtcaggatca aactcgattt
     1561 ccgcaacctg aacgtcgaac ggtaaatcca ccagtaccgg acccggacga ccagaacgca
     1621 tcaggtgaaa tgcctgttgc agcacgcgag gcaccagcgc cgcttcacga actgtaaccg
     1681 ccattttgct gaccggttta gcaattgctt caatatctac ggcctgaaaa tcttctttat
     1741 gcagacgggc gcgcggtgcc tggccggtaa tgcacagaat aggaatggaa tcagcagaag
     1801 cggaatagag cgcggtgatc atgtccgtgc ccgcaggacc ggaagtcccc agacatacgc
     1861 cgatattccc tgccgttgcg cgggtataac cttccgccat gtgcgaaaca ccttccacat
     1921 gacgcgccag aatgtgacga ataccgccgt gcttacgcat cgctgagtag aacggattaa
     1981 ttgcagctcc cggaacgccg aaggcggtgg tgataccttc tttctccagc acatacattg
     2041 ccgcgtcaac ggctctcatt tttgccattt tattcctacc tctattagtt ggaaaaattt
     2101 tccaactttc aaattttgtc aaaaaaaccc gctccgcaga gcgggatgcg acagacacta
     2161 tggatgtgct ttcagtccca acgccgtgct gatatcgcgg gcggtgtctc tgaccagctc
     2221 accctgactg acaaaacgat cttctgtcag tcttgatgaa ggcccggaga tggagatagc
     2281 ggcaacaact ctaccgacat catcgtaaat tgctgaagct atgcaattca gacctacaac
     2341 atgctcttct ttatctacgg tatagcccag ttcacgcgct tgttccaggt ccttcagcaa
     2401 ggtgggcata tccacaagcg tagttggcgt aaactgctgc aaaccggttt gcagaatgat
     2461 gctcatcaac tcctcttccg ccagcggata aagcagcgct ttgcccgcac cggaagcatg
     2521 cagcggcaga cgactgccca gtggcgcaca catcctgacc atcgatttac actctaactg
     2581 accaattaat accgcttcat tgccgttacg gatcgcgaca ttgaccgttt cgccggaaag
     2641 taacatcagg cggcgcataa acggcccggc gacggagagg acatcgcggt tatggatgta
     2701 cgccgcaccg acgttaaaga cacctaatcc tatatgccac cagcctaatt gactgtcctg
     2761 atagacaaaa tccgctgctt gtaaaacctt cagcaagcga aaggtcgtgg agagcggcaa
     2821 atccagattg agagaaatat cgctaaccga cgaacttccc ccactttttt ccagatattg
     2881 cagaatcgca attccccgct ctaacgcctg tgcgcccttc tgtgccacag gctccgcctg
     2941 tcctggcctg ccgcgccgtc taacttccgt catacctacg ttaactccga cctacaagtg
     3001 agcataaaca atgaacaatt agtttatctt atgcaagtcg gttgcaggcg tcacgcaaaa
     3061 cagagttcct gttcaggaat actttcaaca tcacagttgt cactgccgcc gcgatcgatg
     3121 gtcagaaaat cggtgacgcg ctgccaggcg aaaagtgggt gatgccagac gttacgatgg
     3181 taattcaccc cctgttcacc gttggtgata aacgcccgca gcgttgacag gtctggtttg
     3241 tcgtcgccta acgccacgac caccacaaac acttcacctt tcattgggat aaaggcctga
     3301 gtgcccagcg gatgacgttc gagttcgtga atggtcagcg gcagattcgc cggttgcgcg
     3361 cggttaatgc tgataagcgt acggtcttgc tcaagaatct caaccagcgc caaatcgtgg
     3421 taacgctcca ccaggccatt gttaatatgg aaaaaatccc gttgctgcgt ttcgattacg
     3481 tcgccataag cactaaaggc ttcctgactt aacggtaata cctgaagttt catctttttg
     3541 tccttgtaac ataattccgt tcaaccaccg ccgaccggtc agcttggttt caataacagg
     3601 caatctaaaa ccttaagaga acaacattaa tacaaaagtt ggaatgtttt tctaaaacta
     3661 gatttagtcc acaaattgaa caggattatc atgtaaaaat actgataaat cagaggataa
     3721 gaaaatcacg caacttgcgg atttaaaagg ggatacctga agatgttcga tccagaaacc
     3781 ttgcggactt tcattgcggt tgctgaaaca ggaagttttt caaaagcggc agaacgatta
     3841 tgtaaaacca cggcgacgat cagttatcgc attaaacttc tggaagagaa taccggagta
     3901 gcgctgtttt tccgtacgac tcgtagcgta acgttgacag cggctggcga gcatctactt
     3961 tcccaggcca gagactggct gagctggctg gaaagtatgc ccagcgagct gcaacaggtg
     4021 aatgatggcg tggaacgcca ggtgaatatt gtcatcaaca acctgctcta taacccccag
     4081 gccgtcgccc agttgctggc gtggctgaat gagcgttacc cctttaccca gtttcacatc
     4141 tcccgacaaa tctatatggg cgtctgggac tcgctattgt acgaaggttt ttcgctggct
     4201 atcggggtca cgggaactga ggcgctggca aataccttta gtcttgatcc cttaggatcg
     4261 gtgcaatggc gatttgtcat ggcggcggat catccgctgg cgaacgttga agagccgcta
     4321 acagaagcgc agttgcgtcg ctttccggcg gtcaatattg aagacagcgc cggcacctta
     4381 accaaacgcg tcgcctggcg attgccgggg caaaaagaga ttattgttcc cgatatggaa
     4441 acgaaaatcg ccgcccatct ggcgggtgtt ggcattggtt ttttgccaaa atcactttgc
     4501 cagtcaatga tcgataatca acaactggtc agccgggtaa tcccaacgat gcgccctcct
     4561 tcgccattga gtctggcatg gcgcaaattt ggcagctgca aagcggtaga agatattgtg
     4621 accttgttta cccaacacag gccggaaatc agcggatttt tagaaatttt cggcacccca
     4681 cgcagttaaa aatcaagttt ccttgcgcgt tggcgcgtaa aatagcgcgc aagctgattt
     4741 gaggttcact tttaactatg caagagagac acacggaaca ggactatcgt gccctgctga
     4801 ttgctgatac gcccattatt gatgttcgcg cccctatcga gtttgagcaa ggcgcaatgc
     4861 ccgccgctat caatctgccg ttaatggata acgatgaacg cgcctccgtt ggcacctgct
     4921 ataaacagca aggctcagac gcagcgctgg cgctggggca taaactggtg gcgggtgaaa
     4981 ttcgtcagca gcgtatgtac gcctggcggg cagcgtgcct gcaaaatccg caaggtattc
     5041 tctgctgcgc ccgtggcggt caacgttcac atattgtgca acgctggctg catgaagcgg
     5101 ggattgatta tccgctggtg gaaggcggtt ataaggcact gcgccagacc gcgattcagg
     5161 cgaccattga actggcacaa aaaccgatag tgctgattgg cggttgtacc ggcagcggta
     5221 aaacgctatt agtgcaacaa cagccaaacg gtgttgatct ggaagggctg gcgcgtcatc
     5281 gcggttcggc gtttggtcgc acgttacaac cacaacttag ccaggcgagt tttgaaaacc
     5341 tgctggctgc cgaaatgcta aaaaccgacg cccgtcagga tttgcgcctg tgggtgctgg
     5401 aagatgaaag ccggatgatc ggttcgaatc acctgccgga atgtctgcgc gagcgaatga
     5461 ctcaggcggc gattgcggtg gtagaagatc cgtttgagat ccgtcttgaa cgcctgaatg
     5521 aagagtattt cttgcgtatg catcatgatt ttacccacgc ttatggcgac gaacagggct
     5581 ggcaggaata ttgcgaatac ctgcatcacg gactttcggc gattaagcgt cggctggggt
     5641 tacagcgcta taacgaactc gctgcccagc tggatacggc gctgacaacg caactcacca
     5701 ccggcagcac cgacggtcat ctggcctggc tggtgccgtt acttaaagaa tattacgacc
     5761 caatgtatcg ctatcagctt gagaaaaaag cggaaaaagt tgtctttcgc ggtgagtggg
     5821 cggaagtggc ggtatgggtt aaggcgcagt gatgggcggt attctgaaac ggcgattgtc
     5881 gtgagaacaa aatggttgca ccagaataca gatataaaaa aaccgacatg acagtcggtt
     5941 tttttatttt ttccgcccaa gacttggtgg ttccgggcag aaattaaaca gtcctcatct
     6001 agcaagtgaa ttataagtca ggatgcctac atattcaaac agctatgttg gcgatacaat
     6061 cccgacgcta gtagtgatac tgcctcaagg gattaccgca gtgcggaaaa gtacttttaa
     6121 gtcctggtca ggatacaggt ggtgctgatt ctggtgctct aatttggaga gagacagtcc
     6181 tcatctagca catgaagaaa atcatagtta taggcaacgt aagggtaagc tgtgattgtc
     6241 tttctccctt aagggagcaa cggatgaaaa cttttctcga taaagttctc atcgttctta
     6301 aggcgctaat cgcgctgctg gaactgatcc gtcagtttat tgaataactg aggaaacgta
     6361 gctaaggccg aaggtttacc tccttcggcc tttaaaaaaa ccgacatgac agttggtttc
     6421 atatttattg ccaaaatgca cactgttcat gccagatgcg gcgtaaacgc cttatccggc
     6481 ctacacaatc ttgcaaaatc aatatattgc acgctcttcg taggcctgat aagcgtagcg
     6541 catcaggcaa tctaacacct gtcatcaccc cacaaactgc ctgaacagcg ccttaccctt
     6601 aaccagtcgc gcacccagcc agccgccgaa aagcgacagc agcagcgcac cgctgcacgg
     6661 cagaacaatc cacaatcgcc agtctggctc ccacgggaaa tcaaacactt tcgcctgcaa
     6721 cactgccagt gccgtttccg caccaattgc ggccaccagg ccggaaacaa acccaagcat
     6781 ggcgaacatc acaccacaat gtcgtgcgca gcagtttttt ccccgcaccg agtgtgcgcc
     6841 ataccaccag ctcctgatga cgctggcgca ttcccacctg cacctgtgcc agcaacagca
     6901 acataccgca ggcggtgacc agcaccacca tcacctccag cgcccgactt acctgctcca
     6961 gcacctgacc gacctgtttt aaaatcgcgc caatgtctaa caggctaatg gtcgggaact
     7021 ggcggttgag ttgcgtcaac atgccgttgc cattctccca gcggaaactg gtaagccagc
     7081 tctgcggttg cccgtctaat gccccttcag ggaaaataaa atagaaatta ggccgcagac
     7141 tttcccagtc cactttgcgc aggctggtca ctttagcgcg gaactcctgg gtatcgccca
     7201 taaaagtcac ggtatcaccg agggcaacgt ttaagcgttt cgccagcccc tcttccatcg
     7261 acacttcatc ggcttttggc ggccagttac cggcgacaat cggattatga tcgggccgcg
     7321 tattttgcca ggtaagattg agttcgcgat taagcgcctc atcttcatta ccttctgtcg
     7381 gcttatcatt aatcgccgtc agccgcgccc gcactaccgg ataaaatgat tccgggacta
     7441 tatgatgttc cgcgaggaac gcttttagcg gtgctacctg ttctgtggcg atgttaatta
     7501 aaaagtagtt cgggctttct ggcggtagct gctgctgcca gcggtcgagc agatcgccac
     7561 gcagcaccag cagcagtgcc agcagcataa aggagagcga aaatgccgaa agctggctta
     7621 acgttgacca cggctgacgt aacaggcggc taaccgccag gcgcagaggc agcgatttca
     7681 gcgtcatgcg gcgaagtaca ttcagcagca tccagcccag cacaccgcac agcaaagcca
     7741 gtactaccgc gcccgccagc accgcccaaa gcagcatgct gccacccatt aatccggcga
     7801 gcagcagcac aaccaccaca ctgacaatcg gcagataaaa cttcagcggc cagacgttcg
     7861 cgaccacatc attacgtaat acgcgtaaag gctgcgttgc cagcaacaag cggtatggtc
     7921 gtagccccac cagcagcgag atgaccgtca tggtgccaag cgcccacagc cacggccaga
     7981 ggctggctgg cggtagtgca gcaggcagaa cgggcttgag cagcaccatc aacacgtttt
     8041 cgaacaacag gcctatggcc ccaccggtaa cggctgaaag cgtcagcacc atcaaccact
     8101 gaccgacgat tagcttacgc agttgcgctc gccctgcccc cagcgttttg aggatcgcca
     8161 ccagatcgta gcgactgcga cagtaatgat tcatcgctac cgccaccgct gccactgcca
     8221 gcagcaaggt cagaagcgcc gaaagcagca ggaactgttg cgagcgttcc atcgatcgcc
     8281 ccagcgcgcc ttcgtcctgt tccagaccgt accagcgttg ttcgggttta agctgaggta
     8341 acaaccattt ctcatagccg tcgagctggt tctcgttgcc gccgaattta taacgccagg
     8401 tgacccgact ccccggctgc acggctccgg ttttatcgac atccgccaga ttcatcatca
     8461 gacgcggagc catctggaag gggttaaaac cggaatccgg ttcctgaatc acttctccgg
     8521 caatccgcaa ggtggcatcg cccacgtcaa tggtgtcgcc cgttttcagg ttaagcagtg
     8581 ccatcaggcg tggggccagc aatacgctgc ccgcctgcgg tttcaggcca gggggattag
     8641 tttgcagacc gccatacatc gggtagatat catccaccgc tttgacgttc gccagctgcg
     8701 gtgtgtcgcc tgcaaaggtc attgtggcga aagtcagctg cttgccgact ttcaggccgc
     8761 gcttttgcgc ttcctccagc cacgcttgcg gcacttcgcg tgaactgcgc aacgcccgat
     8821 cgcccgccat aaactcacgg ctttgctggc ttaagccctt ctccatgcga tcgctgatat
     8881 tgcccagcgc cagcacgcag gccaccgcca ggcttagcgc cagccagaca attaatagcg
     8941 acggcgaacg ccattcgcgc cagaaccaac gtgcaatcat gcttcctcct gcaactgccc
     9001 gttcaccagc cgtaagcagc ggtcgcagcg tgccgccagt tgcaggtcgt gggtcaccat
     9061 aatcaacgtg gtgccatgtt cacggttgag ggaaaacagc aggtcggcaa ttttatcgcc
     9121 cgtctggcgg tcaaggttgc cggtgggttc gtcggcaaac agcacatcag gtcgaccatt
     9181 aaaggctcgt gccagcgcca ctcgttgctg ttcaccgccg gaaagctgtg ccggaagatg
     9241 atccagacgt ttacccagcc ctaactgttc gagcaacgct ttcgccccgt tacgactttc
     9301 cgcgctactc tcaccgcgca gcagagccgg aagctcgacg ttttccagcg cgttaagggt
     9361 aggaattaac ataaatgact gaaaaacaaa gccgacgtgc ttcgcgcgca actttgcccg
     9421 cgcttcttcg tccatattat gtagcggttg tcccaccaga ctcacttcgc cactgctgcc
     9481 gtcatcaagc ccggcgagga tcgccagcaa ggttgactta cccgatcccg actcgcccac
     9541 cagtgcgatg gtctcgccac gtttgacaac cagctcaact ccggtgagga tggagagttc
     9601 atgctccccc tgaccgacgg acttcttaag atgatgaact tcaacaatgt tttccgctgg
     9661 catttgccct tcctgttcct ggtcctgtta accttccgtg ccgccgcagc ggacacgtta
     9721 ttgattctgg gtgatagcct gagcgccggg tatcgaatgt ctgccagcgc ggcctggcct
     9781 gccttgttga atgataagtg gcagagtaaa acgtcggtag ttaatgccag catcagcggc
     9841 gacacctcgc aacaagggct ggcgcgcctt ccggctctgc tcaaacagca tcagccgcgt
     9901 tgggtgctgg ttgaactggg cgcaatgatg gtttgcgtgg ttttcagcca cagcaaaccg
     9961 agcaaacgct gcgccagatt ttgcaggatg tcaaagccgc caacgctgaa ccattgttaa
    10021 tgcaaatacg tctgcctgca aactatggtc gccgttataa tgaagccttt agcgccattt
    10081 accccaaact cgccaaagag tttgatgttc cgctgctgcc cttttttatg gaagaggtct
    10141 acctcaagcc acaatggatg caggatgacg gtattcatcc caaccgcgac gcccagccgt
    10201 ttattgccga ctggatggcg aagcagttgc agcctttagt aaatcatgac tcataaagca
    10261 acggagatcc tgacaggtaa agttatgcaa aaatcggtct taattaccgg atgttccagt
    10321 ggaattggcc tggaaagcgc gctcgaatta aaacgccagg gttttcatgt gctggcaggt
    10381 tgccgaaaac cgaatgatgt tgagcgtatg aacaacatgg gatttaccgg cgtgttgatc
    10441 gatctggatt caccagaaag tgttaatcgc gcagcagacg aggtgatcgc cctgaccgat
    10501 aattgtctgt atgggatctt taacaatgcc ggattcggca tgtatggccc cctttccacc
    10561 atcagccgtg cgcagatgga acagcagttt tccgccaact ttttcggcgc acaccagctc
    10621 accatgcgcc tgttacccgc gatgttaccg cacggtgaag ggcgtattgt gatgacatca
    10681 tcggtgatgg gattaatctc cacgccgggt cgtggcgctt acgcggccag taaatatgcg
    10741 ctggaggcgt ggtcagacgc gctgcgcatg gagctacgcc acagcggaat taaagtcagc
    10801 ctgatcgaac ccggtcccat tcgtactcgc ttcaccgaca acgtcaacca gacgcaaagt
    10861 gataaaccag tcgaaaatcc cggcatcgcc gcccgcttta cgttgggacc ggaagcggtg
    10921 gtcgacaaag tgcgccatgc ttttattagc gagaagccga agatgcgcta tccggtaacg
    10981 ctggtgacct gggcagtaat ggtgctcgag cgcctgctgc ctgggcgcgt gatggacaaa
    11041 atattgcagg ggtgagttga agcgcgcgct taagccccca tgtcaaagaa aatatcgaca
    11101 acagagagcg actccatgtc cgtagaaaat attgtcaaca ttaacgaatc taacctgcaa
    11161 caggttcttg aacagtcgat gaccactccg gtgctgttct atttttggtc tgaacgtagc
    11221 cagcactgtt tgcagttaac cccgatcctg gaaagcctcg cggcgcagta caacgggcaa
    11281 tttattctgg cgaagctgga ctgcgacgcg gagcagatga ttgccgccca gtttggtctg
    11341 cgcgcgattc cgaccgtgta tctgttccag aacgggcaac cggtagatgg cttccagggg
    11401 ccgcaaccgg aagaggcgat ccgcgccctg ctggataaag tgctgccgcg cgaagaagag
    11461 ctgaaagcgc agcaggcgat gcaactgatg caggaaggca attacaccga tgccctgcca
    11521 ttgctgaaag acgcctggca gttgtcgaat cagaacgggg agatcggcct gctgctggca
    11581 gaaacgctga ttgcgctgaa ccgttctgaa gatgcggaag cggtgctgaa aaccattccg
    11641 ttgcaggatc aggacacccg ctaccagggg ctggtagcgc aaatcgaact gctgaagcag
    11701 gcggctgata agccggaaat tcaacagttg caacagcagg tggcggagaa tccagaagat
    11761 gccgcactgg cgacgcaact ggcgctgcaa ctgcatcagg ttgggcgcaa tgaagaggcg
    11821 ctggagttgc tgttcgggca tctgcgtaaa gatctcaccg ccgcagacgg tcagacgcgt
    11881 aaaacgttcc aggagatcct cgctgcgctg ggtacgggtg atgcactggc gtcgaagtat
    11941 cgccgccagc tgtatgcatt gttgtattga tattccgttg ttcggcacat gaaccacaac
    12001 gccggatgcg atgctgcgca tccggcatcg catcatttct tcttcaattg agtcaccacc
    12061 aactggtggc gcgaattata aaacttacga taggttaaat agcaggcaat aatggtcgac
    12121 aagctggcgg ttgagagcag cataaaggtc accataatct gatatttaat cgccttcacc
    12181 ggatcaatcc cggcaaatat cagcccggac atcattcctg gtaaactcac taagccaacc
    12241 gtttttgctg aatcgaccgt cggaattaaa gccgcgcgaa tactgtcgcg aatcaatatc
    12301 gctgaagcct gcttcggcgt cgcgccaaga ctcagtttct cctgaatctg ttgctgttcg
    12361 ctaatgaccc gttgccctaa attgttgtaa cacaacccca ccgctaccat ggcgttaccg
    12421 gcaatcatcc cggcgacagg gatcacctgc atcgggataa attcaatcgc ccctgagaga
    12481 atcagcaccg ccagggtaat tcccgccccg accgtaatgg caataaacga tgagataaaa
    12541 gctttagcaa tatatttact gcgtttttgc gcgttccacg ccgcattaaa gcagataaat
    12601 aacaccatca ataatgtcag gctggcatca tccacgctga aaatatactt cagcacatag
    12661 ccgacaataa tcagctgaat tatcgctcgc ccgacgctcc atagaatatc tttctccagc
    12721 gccagttttt ctttatggct aattaagatt gccaccacca ccagcattaa tgccagtgct
    12781 aatgattcgt tagtaatatt atgcgagttc atagcgtgct tcctgcattt ctccggcatg
    12841 cggttgcagt gtaatcactt tatccgcatg attaatttcg tctttatcgt gtgtcaccca
    12901 cagcacggca atattttgct cgcgcacata acgatggatc atctcattga cgttatgttt
    12961 attactttca tccagcgcac tggttatttc atccagcaat aaaaccttcg gcataaattg
    13021 caggttacga atcaatgaga tgcgttgttt ttcaccacca gatagctcgg cgatattctt
    13081 cgtcaaaatg ctgtccggca aagcgaagcg ttcgagaaaa tcgagaaaaa tggccgggtc
    13141 aggctgctgg tcacggatct gccagggaaa gatcagatta tcgtataccg tatcgccaaa
    13201 cagcgtcggc gtctgggcgc agtaagagac ttgctggcgg tagatttctg gttttagtgt
    13261 gctgacatcc tcaccttcaa acagtaacgt tccgctggtt gggctgatca atgaagcaac
    13321 tatttttagc agcgtacttt tgccacaacc agaaggaccg gtaattaact taaattcgcc
    13381 agcacgcagc gaaaaattga tgttattaag aatcttcgta tcacccgcca gatatcctac
    13441 gttttgtagc tgaagcaaag gactattttc ctgcatcgct gttccctttt tctgattttt
    13501 actaaaaaca gtttatcctt cgcaggaata agggggaact ctctttcagt aatcaggtat
    13561 aatttgttta atcaagcagc aattttgctg attatggacg gtacaacagg aggtttttcc
    13621 gatgcttatc tttatcccga ttctcatttt tgtcgcgctg gtcattgtcg gcgcgggcgt
    13681 caaaatcgtg ccgcagggct atcaatggac ggtagaacgt tttggtcgct ataccaaaac
    13741 gttacagccg gggctcagtc tggtggtgcc gtttatggat cgcattggtc gcaagatcaa
    13801 tatgatggag caagtgctcg atatcccttc ccaggaagtt atctcgaaag ataacgccaa
    13861 cgttaccatc gacgcagtct gttttattca ggtgattgac gcgccacgcg ctgcctatga
    13921 agtcagcaat ctggagctgg cgatcatcaa cctgaccatg actaacatcc gtaccgtgtt
    13981 gggttcaatg gaacttgacg aaatgctctc tcagcgcgac agcatcaact cacgcctgct
    14041 gcgtattgtc gatgaagcca ccaacccgtg ggggattaaa gtcacccgta ttgaaattcg
    14101 cgacgtgcgc ccaccggcag agcttatctc ttcaatgaac gcgcagatga aagcggaacg
    14161 taccaaacgc gcttacattc ttgaagcgga agggatccgt caggcggaaa tcctcaaagc
    14221 cgaaggtgaa aaacagtcgc aaatcctgaa agcggaaggc gaacgtcagt cggcgttttt
    14281 acaggctgaa gcgcgtgaac gttccgccga agcagaagcc cgcgccacca aaatggtgtc
    14341 tgaagctatc gcctccggtg atattcaggc ggtgaactac ttcgtagcgc aaaaatacac
    14401 cgaagcgtta cagcagatcg gttcctccag taacagcaaa gtagtgatga tgccattaga
    14461 ggccagcagc ctgatggggt cgattgccgg gattgccgag ctggtgaaag acagcgccaa
    14521 caaacggact cagccatgat ggagttaatg gtcgttcatc cacatatttt ctggctcagt
    14581 ctcggcgggt tgctgctggc agccgagatg ctgggcggaa atggttattt gttgtggagc
    14641 ggcgtggcag cggtgattac cggcctggtg gtctggctgg tgccgctggg ttgggagtgg
    14701 caaggggtaa tgtttgccat cctgacgctg ctcgccgcct ggctgtggtg gaaatggttg
    14761 tcgcggcggg tgcgcgaaca aaagcacagc gatagtcatt taaaccagcg cgggcagcag
    14821 ctgattggcc gacgttttgt gctggaatct ccgctggtca acgggcgcgg tcatatgcgc
    14881 gtcggtgaca gttcatggcc tgtcagcgcc agcgaggatc tcggtgcagg tacgcatgtt
    14941 gaagtcattg cgatagaagg gataacccta catatccgag ccgtctcgtc ttaatcaccc
    15001 tgcccgatga tgacagcagc cggagagatt ttcgataatc gggcagtcgg cgctgtcatc
    15061 gccagggcag gcattcgcca gtgccagcag ctggtcgcgc atggattgca gctcctcaat
    15121 gtgtcgttcg atctccgcca ccttctccag cgtgcgccgt ttgacgtcgg cgctgtgccg
    15181 ctgcgggtcg ttaaacagat tcaccagctc gccgctctct tccaggttaa agcccacctg
    15241 ccgtgcctgg cgcagtaagg tcagttcgtt gagatgctgc tgcgtgtagg tgcgataacc
    15301 gttttcgctg cgcatcggcg gcgtcaccag ccccttctct tcatagaagc gaatggcttt
    15361 gctggtcagg ccggtaattt ttgctacatc gctgatgttc atcgttcgcg caacgcctcc
    15421 tgtgctttgt taaagggttt caacagatat tcaagcacgt ttttttgcca gtgcgaatat
    15481 cgaccgccac cacaataata caggaagtac tgcgcacaga gaggtgttat acggttttat
    15541 tacgcttcat gaccattgcc acaataaggc tgagtaagat tgcaccaata atccatatca
    15601 acgcacggct gccctgggac cacaggctgt agataaagcc aacgatcacc aacaacatca
    15661 ccaacgtacc aacgataaga atcggcaacg aggcgtgaat atcatgacgc aggcggatcg
    15721 ccaccacaaa caccgccaga tagcagatca aaaaggtggc gctggcaacg ctggcgagtg
    15781 agtctaaatt tagcgccgcc gtcatcagca ttatcaacac cacgacgatg atattgcccc
    15841 aggtactctg ccgccacagg gatttattca ttagcttcgg cagttcgcgt tcgctgccca
    15901 tgttgtccat gatgttaaac acggcgaaca ggttcgcgtt aatgcctgaa gccgtcgcca
    15961 gtaaagcgcc gatgacgacg atcacataac ccacatgccc gagcagcgga gaagcagcct
    16021 gcgctacggc ggtatcagca tatttttcta actctaatgc cgatacatcg ctaagcaaaa
    16081 ccagcgccag cgagatataa agcaacgtgg taacgccaat cgccaccaga aacgcccgtg
    16141 gcataatgac ctgcggatct ttcactttat ccgccgcgtt cgccatcatg ccaaagcccg
    16201 cataggcaag gaaggttatc ccaatacagg agaagaacgc accggagctg gggggcgcag
    16261 agacggaaat atgcgccggt tgcagcgacc agacaccggc aataatcagc aataacagga
    16321 tcatcatttt aatgccgacg aggatcactt ccagccgccc taccgcatgg ttgcttaagg
    16381 agttgaaaag cgtcatcacc gcaatgatcc ccaacgcgta gagcaaaata aggtgctcct
    16441 cctggctgcc ttcatgcaaa aactgcacgg cataagcgcc aaaagcacgg gcaaccatgg
    16501 cgatgctcac cgccagcgtc aacaggtata gcaacgagag cgctagcgaa aagacgccgt
    16561 tgcctaatcc gcgacgaaaa aagtcgataa taccgccatt gctgggatag ctcgccccca
    16621 gacgcgcata ggcataaccg gaaaacatag ccacaatacc gccaaaagca aaggcgcccc
    16681 aggtcgaggc ttccattagc aacgcagcct gccccagcag cgcgaagatc cccgccccca
    16741 ccattgcccc aataccgatg gaaacgacgt tccatagacc gaggggtttg ttaccgttat
    16801 taccttccgt gttcatcatg atcagccctt aaacacgtta tagccgagtt gcttagcgac
    16861 cgatgccacc attttttgac cgcgaacact attgccttct tcgtccagcg gtggtgagaa
    16921 cgcggcaatt cccatcacgc cagggacgac cgccagaata ccgccaccta caccgctttt
    16981 gcccggtaaa ccaacgcgat acgcccagtc accggagcga ccatacagcc cttccatcat
    17041 catttcggcc agaatgtacg gcacgttgtc ggcctgaaga acgcgtttat gcgtcaacgg
    17101 attcacacca cccgccgcca gcgtcgcgcc aagcgttgcc agttcaacag tattgatgag
    17161 cgtagagcac tgacgggtgt acacgtcaca ggcttccatt gcatcacaat agagatatcc
    17221 ggcggagtac agcagccagg caatggcccg attatggaag ttggttgttt gttccgactg
    17281 gttgacttcg tcagagagcg ctacctgctc accagccagt tgctgttgga tatgtaaaat
    17341 tcgctgccag cgttgttcgg cgttttcagc gttaatcagg ctggtggtgg caatagcgcc
    17401 agcatttacc agtggcgaaa gcggtttgcc gccatgcaac tctaaggcga taactgagtt
    17461 aaagggcaat ccggtcgggt cagcgccaat tttgtcctgt accgcctgcg ggccgacatc
    17521 ttctaacgca agggctaacg tacagacttt agagatggat tccagtgcaa agcggtaatc
    17581 actgtcaccc gcactataga cgttgccatc gcaggtcacg atagccactg ccgccagttg
    17641 acctggtaca ttcgccagaa agggaatgta atcggcattt tgtccgccgt taagtgagtg
    17701 aaattgggtg taagcctgat ccactgcctg ctgtaatttg tttgcatcta acatcttttg
    17761 ttaactcctt tttatagatg cgggaggtaa ttcctcaccc cagtgccgat tttcaggcat
    17821 cctgatttaa cttagcaccc gcaacttaac tacaggaaaa caaagaaata aatgtctaat
    17881 cctgatgcaa atcgagccga ttttttaatc tttacggact tttacccgcc tggtttatta
    17941 atttcttgac cttccccttg ctggaaggtt taacctttat cacagccagt caaaactgtc
    18001 ttaaaggagt gttttatgtc acaaactatc gacctgaacc tggacggcct gtcctgcggt
    18061 cactgcgtta aacgcgtgaa agaaagtctt gaacagcgcc cggatgtgga acaagcggat
    18121 gtgtctatca ctgaagcgca cgttaccggg actgccagtg cagaacagct gatcgaaacc
    18181 atcaaacaag cgggttatga cgcatctgta agccacccaa aggctaaacc gctggcggag
    18241 tcatcaatcc cgtcggaagc actgacagcg gtttctgagg cgcttccggc agcgaccgcc
    18301 gatgacgatg acagccagca gttgctgctg agcggcatga gctgcgccag ctgtgtcacc
    18361 cgcgtacaaa atgcgctgca aagcgtaccg ggcgtcactc aggcacgggt aaacctggcg
    18421 gagcgtactg cgctggtgat gggcagtgcc tccccacaag atttagtgca ggcggtggaa
    18481 aaagcgggct acggcgcgga agcgattgaa gatgacgcga aacgccgcga acgccagcaa
    18541 gaaaccgccg tcgctacgat gaagcgcttc cgctggcagg caattgtcgc actggcggtg
    18601 ggtatcccgg tgatggtctg ggggatgatc ggcgataaca tgatagtcac cgccgacaac
    18661 cgcagcctgt ggttggttat cggcctgata accctggcgg tgatggtttt cgccagcggc
    18721 catttttacc gcagtgcatg gaaaagcctg ctgaacggtg cagcgacgat ggatacgctg
    18781 gtggcgctgg gaacgggcgt ggcgtggctc tattcgatga gcgtcaacct gtggccgcag
    18841 tggttcccga tggaagcgcg acatctttac tatgaagcca gtgcgatgat tatcggtctg
    18901 atcaatctcg gccatatgct ggaagcacgc gcacgtcagc gttcttctaa ggcactggag
    18961 aaattactcg atttaacccc accgacggcg cgcctggtta ctgacgaagg tgaaaaaagc
    19021 gtgcctctgg cagaagtgca gccaggtatg ttgctgcgcc tgacgaccgg cgatcgcgtg
    19081 ccggtagatg gcgagattac ccagggcgaa gcatggctgg atgaagcgat gctgacgggc
    19141 gaaccaatcc cgcagcaaaa aggcgaaggc gatagcgtcc atgccgggac agtggtacag
    19201 gacggcagtg tgctgtttcg tgccagtgcg gttggcagcc ataccacgct atcaaggatc
    19261 attcgcatgg tgcgccaggc ccagagcagc aagccagaaa tcggtcagct ggcggataaa
    19321 atctcagcca tatttgtgcc ggtagtagtg gttattgcgc ttgtcagtgc ggcaatctgg
    19381 tatttctttg gtccggcacc gcagattgtc tataccctgg tgattgccac tacggtgctg
    19441 attatcgcct gcccgtgtgc gctgggactg gcgacgccga tgtcgattat ttccggcgtc
    19501 gggcgggcgg ctgagtttgg cgtgctggtg cgggacgccg acgcgctgca acgcgccagt
    19561 acactcgata ccgtcgtgtt cgataaaacc gggacgctga ccgaagggaa gccgcaggtt
    19621 gtcgcagtga aaacatttgc tgatgttgac gaagcgcagg cgctgcgtct ggcggcggcg
    19681 ctggagcaag gttccagcca tccattggca cgagcgatcc ttgataaagc aggcgatatg
    19741 cagctaccgc aggtcaacgg tttccgcaca ttgcgcgggc tgggcgtgag cggtgaagca
    19801 gaaggtcatg cgttattgct gggtaatcag gcgttgttaa atgatcagca agtcgatact
    19861 aaagcgattg aagccgatat ttcagctcag gcatcgcaag gagcaacgcc tgtgctgctg
    19921 gcgattgacg ggaaagcagt agccctgctg gctgtacgcg atccgttgcg tagtgatagc
    19981 gtggcggcgc tgcaacgcct gcataaagcg ggatatcgtc tggtgatgtt gaccggggat
    20041 aacccaacca ccgccaatgc gatcgccaaa gaagctggaa ttgatgaagt gattgccgga
    20101 gtgctgccgg acggtaaagc cgaagcgatc aaacgtctgc aaagtgaagg acgtcaggtg
    20161 gcaatggtgg gcgacggcat taacgacgcg ccagcgctgg cccaggcgga tgtcggcatt
    20221 gcgatgggtg gcggcagtga tgttgccatt gaaaccgcgg cgattaccct gatgcgccat
    20281 agcctgatgg gcgttgcgga tgcgctcgcg atttcccgcg caacgctgcg caacatgaag
    20341 cagaacctgc tcggtgcctt tatctacaac agcatcggta ttccggtcgc cgccggtatt
    20401 ttgtggccgt ttactggaac actgcttaac ccggtagttg ccggagcggc aatggcgctc
    20461 tcgtcaatta ccgtagtaag taacgccaac cggttgctgc ggtttaaacc gaaggaataa
    20521 gtacgtcatt gcggatgcgc tgctttaggg cggcgcattt ggcatcgacg catgactgcc
    20581 ggatgcggcg taaacgcctt atccggccta cggttcaggc acaggtttgt aggcctgata
    20641 agacgcgtaa gcgtcgcatc aggcaatggt gctcggatgc cggatgcggc gtgaacgcct
    20701 tatccggcct acgagatgaa gcgatgtttt ggccttacgc gacctttttt gcacgctctt
    20761 cacggcgtgc caaatattca gccactgttt caatccgtcc caattcttcc tgggtgcgca
    20821 tgttgttttc aacttcccaa agatcaactg ccgcctggag gtttagccta aaatcgactg
    20881 tggtatcaaa aactttcgcc agacggaatg ccatctcagt ggtgagttta cgattgttat
    20941 tgatcagtgc actgacgcga ttacgatgaa catgcagcaa ctctgctaac tcattgattt
    21001 tcaaatcgag cggttccaga tattcataga gaagaatatc tccgggtgtc gtcggttttc
    21061 ttgttgcctg tttcatggtt tttaactgtt gcttccctgt gaatagcgat gcaagaacat
    21121 actgaatcat acaaacacat taaacaagtc cccaactcca tttgcgccat ccctcaccgc
    21181 gcgctagcat gatatttcac aaagggaggt cgtatggatc tgttgtaccg ggtaaaaacg
    21241 ctttgggccg cgctgcgcgg taatcattac acctggcctg ccatcgatat caccctcccc
    21301 ggcaatcgcc attttcatct gattggcagt attcatatgg gtagccacga tatggctccc
    21361 ctgcccaccc gtttgctcaa aaagctcaaa aacgccgatg cgctgatcgt cgaggcggat
    21421 gtttccacca gcgatacgcc ttttgctaat ttgcctgcct gcgaggcgct ggaagagcgc
    21481 attagcgagg agcaactcca aaacctgcag cacattagcc aggagatggg catttctccc
    21541 tcactctttt ctacccaacc gctgtggcaa atcgcgatgg ttcttcaggc gacgcaggca
    21601 caaaaactgg ggctgcgggc agaatacggt atcgattacc agctattgca ggcggcgaag
    21661 caacaacata aacccgtgat tgaactggaa ggggctgaaa accagattgc catgttgctc
    21721 cagctccctg acaaaggact ggcgctgctg gacgatacgc tgacccactg gcataccaac
    21781 gcacggttgc tgcaacaaat gatgagctgg tggctgaatg caccgccgca aaataatgat
    21841 ataacgctgc ccaatacgtt cagtcagtcg ctgtacgatg tgctgatgca tcagcgaaat
    21901 ctcgcctggc gggataaatt acgcgccatg ccgccggggc gatatgtggt cgcggtcggt
    21961 gcactacacc tgtatggaga agggaatttg ccgcaaatgt tgcgctaaaa aaatggccaa
    22021 tatcgctatt ggcccgtcaa agaggaattt cattttttta ttattatgcc gtcactttaa
    22081 gcgacggtgt aactcgattg tcgctcaatc tcaccatcct gccaatactc ttaggcaaga
    22141 tgactctttt ttttggaccg ccatcaggcg tatgcttgct tcgttttttt gtttaaggca
    22201 ggaaaattac catgacaccc gcagttaaat tactcgaaaa aaacaagatt tcgtttcaaa
    22261 tccataccta cgagcacgat ccggctgaaa ccaattttgg cgatgaagtc gtcaaaaaat
    22321 taggtttgaa tccggatcag gtctacaaaa cgctgctggt ggcagtgaac ggtgatatga
    22381 aacaccttgc cgtggccgtt acgccggtcg caggtcaact ggatcttaaa aaagtagcaa
    22441 aagcgctggg tgccaagaaa gttgagatgg ccgatccgat ggtcgcgcag cgttcgacgg
    22501 gatacctggt tggggggatt agcccactgg ggcaaaaaaa acgtctgcca acgattatcg
    22561 acgcccccgc acaagaattt gccactattt atgtttccgg cggcaagcgc ggactggata
    22621 tcgaactggc ggcaggcgat ctggcaaaga tcctcgatgc caaatttgct gatatcgccc
    22681 gccgcgatta agcattgtgc cggatgcaaa catccggcac tttcggatta ctgccagctc
    22741 acctcacctt tcggttcata aacactcaca tccagcggcg agcttttctg gatatacgct
    22801 ttcagcactt cggcatcaat aaagccggta ttcacatagc ccggtttgtt atcaaggcgc
    22861 ggatatccat caccgccggt ggcattgaag tttaatgtcg ccatacggta agttttcgcc
    22921 ggatcgaccg gttcgccttt gattttaagg tcgttcagtt tgccgtcttt cgccacaaag
    22981 ctaacgttgg caaattgcgg gtaggcacct gaatctggct tcatctgcgc gacggcggtc
    23041 aggtaatcaa tcacctcttt accggtcatg tcggcataca ccaccacatt gccgaatggc
    23101 tgcactttca gcacgttttt atagctgata tcgcctgctt cgatagaatc acgaattccg
    23161 cctccgctca tcaccgcaaa gtcggcacca gtgcgatcca tttgggctgc cagaatcaac
    23221 cgccccatat tggtctgtac aaaacgcact ttgtcacgat cgccttccag acgaccattg
    23281 gttttgccta ttttcacttc cagctgcgct ttgcctttgt tctggaacgg tgataacagc
    23341 gagatcattt gctggttttc agcgatttca ggagtgtaaa gcacgcgctc gcttttcccg
    23401 tcttcccagg tcactttctt cttcaggttc accggaatca gctggtagtt aaccattttc
    23461 atttcgccat tacgaaactc aaaatcagcc cgtcccacgt atttgcccca ctcatgcgcc
    23521 tgcacaatcc agatgccgtt ttgttgatct ggtttgcatg gcgtacccgg cacgtaatcg
    23581 acctgttttt tgttttctgc cgccatgcag accggatctt gcgagtgacc accgacgatc
    23641 atcgccagcg atccggcagg cagcgcgcgt gccatctcca catcgcccgg tgcgttagag
    23701 ccgtgctcac cattatcgta atgccccata tgggtcgccg cgataataat gtctggcttt
    23761 tctgtctgtt gcagctcctg aatcaccagc ttcgcttcat cggcgggctt acgaaattcg
    23821 atatcagtga agtattccag gttaccaatt tttgctgtgt catcggttgt cagcccaata
    23881 acggcaattt tcagatcctg acgcttaaac agcgcccacg gtttaaacag gcgctcgcca
    23941 gtacttttct ggtagatatt cgcggaaagc aacgggaact tggcccactt ttcctgctgg
    24001 cgtaatacgg tgagcggatt atcaaattca tgattaccga tcgccatcgc gtcatagccc
    24061 accagattca taccgcgaaa atcaggttcg gcatcctgta agtcagactc gggcacgcca
    24121 gtgttaatgt cgccaccgga aagtagcagc acgctaccgc cttcagccgc aacctctttg
    24181 cggataccat ccaccagcgt tttttgcgcc gccagaccat attcgccata ttcattgcgc
    24241 caaaaatgcc catgatgatc attggtatgc agaactgtaa ttttgtaggt tttatcctgc
    24301 tcatacgcca gagcagtttc actcgccagt gtaaatgtgg ttaacagcgc taacgccacg
    24361 ccccgctgca ataatttcat acttctctcc ctgacctgat ttcaacgtgt tgaaaaatag
    24421 tatgacgcaa catcatagac aagtctgcgt ccggtgaaga taaatctgca tccagattag
    24481 aaattctgta ttcggcgttg agacttcctt cagacgacgc atacaggtat gttagtcggg
    24541 taataatcac aaataagatc attctttaca tcgcaaccct gaaaaagtga ctttcctatg
    24601 gcaatgagtg aacaaaccca gcctgtggcg ggcgcggctg cgtcaacgac caaggcccga
    24661 acatcgtttg gtattttagg tgctatcagc ctctcacatc tgctgaacga catgatccaa
    24721 tcgctgattc tggcgattta tccgctgctt cagtcagaat tttctctgac atttatgcag
    24781 attggcatga taaccctcac cttccagctc gcctcttcgc tactgcaacc agtggtcggc
    24841 tactggaccg ataaatatcc gatgccgtgg tcgttgccaa ttggcatgtg ctttacctta
    24901 agtggtctgg tgctgcttgc gctggcgggc agttttggcg cagttctgct ggcagcggcg
    24961 ctggtcggta ccggttcatc ggtctttcat ccggaatctt ctcgcgtagc ccgtatggct
    25021 tccggcgggc ggcatggcct ggcgcaatct atctttcagg tcggcggcaa ctttggcagt
    25081 tccctgggac ccttgctggc ggcggtgatt atcgcgcctt atggcaaagg caacgttgcc
    25141 tggtttgtgc ttgcggcact gctggcgatc gtggtgttgg cgcaaattag ccgttggtac
    25201 tcggcacagc accgaatgaa taaaggaaaa cccaaagcga cgataatcaa tccactgccg
    25261 cgcaacaaag tggtactggc agtcagcatt ctgttaatcc tcattttctc gaaatatttc
    25321 tatatggcga gcatcagcag ctattacacc ttttatctga tgcaaaaatt cggattatct
    25381 atccagaatg cccagcttca tctgtttgcc ttcctgtttg ccgttgcggc aggtacggtg
    25441 atcggcgggc ctgtagggga taaaattgga cggaaatatg tgatttgggg ctctatcctc
    25501 ggcgttgcgc cgtttacgct gattttaccc tacgcccgcc tgcactggac gggggtttta
    25561 acggtgatta ttggatttat cctcgcttcg gcattctctg ccattctggt ctacgctcag
    25621 gagctacttc cgggacgtat cggtatggtt tctggactct ttttcggttt tgctttcggc
    25681 atgggaggtc tgggagcggc agttctgggg cttatcgccg atcacaccag catcgagtta
    25741 gtctataaaa tctgtgcttt cctgccacta ttggggatgt tgaccatatt cctgcctgat
    25801 aaccggcata aagactgatt tcattgcagc caaaggcaaa ctttggctgc atcgttttac
    25861 agtcgccata agcctttcct ctgttaaacc gccttctgtt ttccgaaccg tgcttttttt
    25921 gcagctaatt ccatttttct gcaaaattca acaattattc ataaacatca tcaatgaaat
    25981 tgtcataaac tattacctgt aattttggtt ttcccagcca aaaatggaca ataaccacca
    26041 ggaaaaggag acggaatgca tcacgccacc ccgcttatca ccaccattgt tggcggcctt
    26101 gtgctcgcct ttatcctcgg catgctggcc aataaactac gtatttctcc tctggtggga
    26161 tatctgttag cgggtgtgct ggcaggacca ttcactccgg gctttgttgc cgataccaag
    26221 cttgcgccgg aactggctga actgggcgtt attttgctga tgttcggcgt cggtttgcat
    26281 ttttcgctca aggatttgat ggcggtaaag tccatcgcca ttcccggcgc gatcgcccag
    26341 atagccgtgg cgacgctgct gggtatggcg ctctccgccg tgctgggctg gtcgttaatg
    26401 accggtatcg tgttcggctt atgtctttcc accgccagta ccgtggtgtt actgcgcgca
    26461 cttgaagaac ggcaattaat agacagtcag cgtgggcaaa tcgccatcgg ttggttgatt
    26521 gtggaagacc tggtgatggt tctgacgctg gtgttgctgc ccgcagtggc aggaatgatg
    26581 gaacagggcg atgtgggctt tgccactctt gcagtcgata tggggatcac catcggcaag
    26641 gtgatcgcat ttatcgccat tatgatgctg gtaggtcgcc gtctggtgcc gtggattatg
    26701 gcacgcagcg cggcaaccgg ttctcgcgag ctgtttaccc tgtcagtgct ggcgctggcg
    26761 ttagggattg cctttggtgc ggtagagctg tttgatgtct cctttgcact cggtgcgttc
    26821 tttgccggga tggtactgaa cgagtctgaa ttgagtcacc gtgccgccca cgatacgttg
    26881 ccattgcgcg acgcgtttgc ggtgctgttt tttgtctcag tcgggatgtt gtttgatccg
    26941 ttaattctga ttcagcaacc gctggcagtg ctggcgacgc tggcaattat tctgtttggt
    27001 aaatcggtcg cggccctgtt ccttgtccgt ctgtttggtc actcccaacg tacggcatta
    27061 accatcgccg ccagcctggc gcagattggt gagttcgcgt ttatcctggc gggactggga
    27121 atggcattga atttactgcc gcaggccgga caaaacctgg tactggcagg ggcgatcctg
    27181 tcgattatgc tcaacccggt actgttcgca ctactggaga aatatctggc gaagaccgaa
    27241 acgctggaag agcagacgct ggaagaggca atcgaagaag agaaacagat cccagtggat
    27301 atttgcaacc atgcgctatt ggtgggttac ggtcgtgtag gcagcctgct gggggagaaa
    27361 ttgctcgcct ctgatattcc gctggtggtg attgagacgt cacgaacccg tgttgatgag
    27421 ctgcgagagc gtggggtccg cgctgtattg ggcaatgcag cgaacgaaga aattatgcaa
    27481 ctggcacatc tggaatgtgc aaaatggctg atcctgacga tccccaacgg ttatgaagcg
    27541 ggtgagattg tggcgtctgc ccgcgcgaaa aacccggata ttgagattat cgcccgcgcc
    27601 cattatgacg atgaagtgac gtatatcacc gaacgtggcg cgaatcaggt agtgatgggt
    27661 gaacgtgaaa ttgcccgtac tatgctggaa ctgctggaaa cgccaccggc gggtgaagtg
    27721 gtgacggggt aaacggtggt tgccggatgt aaaatatgaa aaatatgtag gcatgataag
    27781 acgcgccagc gtcgcatcag gcatcgagag ccaaatgcca gatgcggcgt gaacgcctta
    27841 tctggcctac gaaccgacga taacttaacg atcccagtaa gactcttcct ggctgtcttc
    27901 acgctccggc aagccgcgcg ttaaacgagg tgaatgctgg ttcagtacct gatagctcac
    27961 acggttagca tatttacaca cctgcgctaa cgatgaataa gttaaccagg tgaatttatg
    28021 tttgctggag tttggtacgt tgctacgatg gtagctgttg gcggtaatgt catgcagcaa
    28081 cgctgccaat gcgccatccc ccgctccatt agtgttcatg attttttccg gcccgcccat
    28141 gtacggcgca atgtgcgaat agacacgcag cggattctgg caatccttgt ggcgcatggc
    28201 gcggctaaac tcatactggt tgaattccgc tatagcgccc ggcagcagcg gatgctgggt
    28261 tttacgtttc gcttcgtctt cggtaaagcc cgccatatac aagccgattg gcccggcggt
    28321 gcacagcacc agctctaccc agtccagcgc cttgtcagat gccaacaacg gatcgctttc
    28381 tccggtcaac gcttcggctt catcttcgtt catcgcaagg atagaaacgt ggtctttgag
    28441 gaattgctgc caccactgcg gattctcggc aatgacaaac ttggtgccca gcgtcagcac
    28501 caccggtacg ttatatttct tcgcgtactc aatggctttc atggttgctt ccggcatggg
    28561 ttcacccggc ttgcaacgca ccagatatga ggtgagaacc agtgccgagg ctccggcaat
    28621 cacatcttcc ggaatgcttt cagcccgcag ctggttcatg tggcctggac tgatagcaaa
    28681 ggtacgttcc ccggactcgc caatcagcgt aaagcaacga ccaatcggac catccacgcc
    28741 ttgtagatag ttaagatcgg tacggctgga agtgttacac aggtaacgat aggcataact
    28801 gccaatttca atattgctgc acatgacgcc cagcagcacc gaacggtcgt ccgcgagcac
    28861 cgagtagttg tgcatggtgt taccaatggt gccacccgca aactgatggg taatcaggtt
    28921 tttctgtttt agctcctgat aaagcacttc ggctacgtca tcctcaatca ccagtgaatg
    28981 cccggcgctt aatccataac gctcaatgaa ttcatcatcc actttcgctt caatatcgac
    29041 cagcgtctgg tcaataccta cgacccaggc ggcgctggtt tcgttttctg gctggaattg
    29101 ctgaagcagc ggatcgcgtg catttacagg gaagtaatgt ttggatttac gtttaccggg
    29161 aaatttcatg gttgttttta cgggtggtta actgagcggc gaatgttaac acaatctgac
    29221 gggagcgcgc gagacgcagc cagtttcaat atcagcaggc gggatcgcat tttcatggca
    29281 gtggttcctt acaatgacgt aatttgaaag gagtttttgt atgaagccgg aaaacaaact
    29341 acctgttctg gaccttattt ctgctgaaat gaagaccgtt gtgaatactc ttcagccgga
    29401 tttaccgtcc tggcccgcaa cgggaacgat tgctgagcaa cgacagtatt acacgcttga
    29461 gcgccgattc tggaatgcgg gcgctccaga aatggcaacc agagcttaca tggttccaac
    29521 aaaatatggg caggtggaaa cacgtctctt ttgtccgcag ccagatagcc cggcgacgct
    29581 attttatttg catggaggcg gttttattct cggcaatctc gatacccacg atcgcatcat
    29641 gcgcctgctg gcaagctaca gccaatgtac ggtgattggt attaattaca ccctttcacc
    29701 tgaagcgcgt tttccacaag cgatagagga aattgtggct gcttgttgtt atttccacca
    29761 gcaggcggag gattatcaaa tcaatatgtc ccgcattggc tttgccggtg attccgcagg
    29821 tgccatgctg gcgctcgcca gtgcgttgtg gttgcgtgat aaacagatcg attgcggtaa
    29881 aattgcgggt gttttgctgt ggtatgggct ttacggatta cgggattccg tgactcgtcg
    29941 tctgttgggc ggtgtctggg atggcttaac gcaacaggat ttgcagatgt atgaagaggc
    30001 gtatttaagc aacgacgcgg accgcgagtc accgtattac tgtctgttta ataatgatct
    30061 cactcgcgaa gttccgccct gttttattgc cggggcggag ttcgatccgc tgctggatga
    30121 cagccgtctg ctttactaga cgttagcggc gcatcagcag ccctgtgagt tcaaactcta
    30181 cccaggtacg ctgcatgcct ttttgcatta ttcacggatg atgaaaaccg ccgacgaggc
    30241 tcttcgcgac ggcgctcagt tctttaccgc tcagctttag cgatacgcgg caacaagatt
    30301 agccatcatt tcgatatgtt ccggcgtggc attaagcgct ggaatatatt catatttttt
    30361 cccgcccgca ccgaggaaga cctcacggtt ttgctcggca atctcttcca gcgtctccag
    30421 acaatccgca gcaaagcccg ggcacatcac ctgtatatga cctacgcctt tttctccgag
    30481 cattttcagc gtttcgtcgg tataaggcat cagccagggt tcccggccaa agcgcgactg
    30541 aaaggtcatc atcacttttt ccggtaccat ccccagtgcg gaagccagtt cgcgagtcgt
    30601 tgtgcggcaa cgttgcgggt aatcatcgcc ttcatctgca taacgctggg gaatgccatg
    30661 ataagagagc agcagcagat ccggttcgcc atgtttggca aaagaagcgc gtacgctgtt
    30721 cgccagtgca ttaatgtaat cgtggttatc ggcgtaatca cgaataaacg atatccccgg
    30781 aatgctacgt ttgcgcgcca gaatgcgtgc cagttcatcc cataccgcac cgaccgtaga
    30841 acaggagaat tgcggataaa gcggcagcac cacaatatga tctacatgct ctgccaggag
    30901 ttcatcgacg gagctttcca gtgatggcga gccgtagctc atgcccagcg ccactggcgt
    30961 atccggtaaa cgttgtgcca acgcctgctg ttgctggcgg ctgtaaacca tcagcggcga
    31021 gccgtcttcc atccagacag aggcgtacag cttcgccaca cgcggcgagc gcagcggcaa
    31081 aatcacgccg cgcagcaacg gccaccataa caaccgtgag gtatcaacca cgcgtctgtc
    31141 gcttaaaaat tgtttcagat agcgttttac cgcttcaggt gtgggggcat cgggcgtacc
    31201 caggtttgcc agcaggatac cggttttagt ctgacgcatt accgcctctt atcgattcaa
    31261 cttgttgata attgtagcgg aaaagtgagg aagaagaact aattgctgtg agaggtattg
    31321 cctgaggtgg ataagcagac ctgcccgaaa gcagatctgc tgcggcttga ttagccgagg
    31381 attttttcca gatcagcgcg aacttcagca acctgcttgg taccgtcaac tttcgcgtat
    31441 ttggtgttac ccgcttccgc ttctttggag tagtagccga tcagcggtgc agtcatctga
    31501 tggtattcaa ccagacgttt acgcacggtt tcttcctgat cgtctttacg ggtagtcagt
    31561 tcttcaccgg taacgtcgtc tttgccttct actttcggcg gattgaattt aacgtgataa
    31621 acacgaccag acggcgcatg aacgcggcgg cctacgatac gatcaacaat cagttcgtcc
    31681 ggtacgtcga attccagaac gtaatcaaca ttgatgcccg cttctttcat cgcgtctgcc
    31741 tgcggaatgg tacgcgggaa gccgtccaac aggaaaccat tacggcagtc ttcctgagca
    31801 atgcgctctt taaccagcgc gatcaccagt tcgtcggtga ccagtttgcc agcatccata
    31861 atgtcttttg cttgtttacc cagctcggag ccagatttga ccgcagcacg cagcatatcg
    31921 ccagtggaga tttgcggaat accatatttc tccatgatga actgagcctg agtccctttc
    31981 cccgcgcccg gagcgccaag cagaatgata cgcattgcga aaatcccctt aaaatgtgtc
    32041 gatttttttg aaaaagcgat aaacgatacc accaacaggc gattgcctca agaaaggcac
    32101 ctgaggctga aacggttaat ggcgagataa tttgcggaaa gtacagatga tgaaaagaaa
    32161 aatgccggat gacacaaagg tcatccggca tgacatcagg aaaccagcag ctgattcata
    32221 cgacgaataa acaggttcgg atcttccagc gtgccgcgtt ctgccagcag cgcctgatcc
    32281 agcagcagtt ctacccactc gctgaacttg gcttcatctt cagtatctgc cgcacgtttc
    32341 accagtacgt gatccgggtt cagttcgaag atgtatttca cttctggcac tttctggccc
    32401 gccgcagcga acagtttcgc catctgagtg ctcatttcgt ccgcgtcggt cgaaacgatc
    32461 gctggcgtat cggtcagacg gtgagtcaga cggacatctt tcacgcgctc gccgagcaga
    32521 gctttcacgc ggtcgatgaa cggagtcagt gctttctccg cttctttcgc gctctcatca
    32581 acttcgtcag ccagtttttc cagcgactcg tcaactttag acaccgactg gaacggttta
    32641 ccgtcgaact cagtcagata gttcatcatc cactcatcga tgcggtcgga aagcagcaga
    32701 acttcgatgc ctttcttacg cagcagttcc aggtgcgggc tgctcttcgc tgccgcatag
    32761 ctgtctgcag tgatgtagta gattttctcc tgcccttctt tcatgcggga aacgtagtct
    32821 tccagagaca cggtctgcgc agaagaatcg gtataagtag aagcaaaacg cagcagtttg
    32881 gcgatcgctt cctggttagc gaaatcttcc gccggacctt ctttcagtac caggccaaac
    32941 tgttgccaga aggtctggta tttttccgcg tcgtctttcg ccagtttttc cagcatttgc
    33001 agcacacgct tggtcagcgc attgcgcagg ttacgcgtta ccgtgctgtc ctggaggatt
    33061 tcacgggaaa cgttcagcgg cagatcgctg gagtcaatca gaccacgcac gaagcgcaga
    33121 tagttcggca tgaactgttc agcgtcgtcc atgataaaca cgcgttgaac gtacagtttc
    33181 aggccgtgtt tatgatcacg gttccacata tcccacggag cctgggacgg gatgtacagc
    33241 aggctggtgt actcctgctt accttcaacg cggttgtggc tccaggtcag cggatcgtta
    33301 aagtcgtggg cgatgtgttt gtagaactct ttatactctt catcggtgat ttccgacttg
    33361 ttacgagtcc acagcgcctg cgctttgttg atttttgccc aggagataac ggtttcgcca
    33421 tctttctctt cgcgtttttc gatctctacc ggcagcgcga tatggtcgga gtatttgctg
    33481 atgatggaac gcacgcgcca gtcatcgagg aactcgtctt cgccttcacg cagatgcagg
    33541 gtgatttcag taccacgatc ttctttggtg atatcggcaa cggtatattc accttcgcca
    33601 gccgattccc agaagacgcc gttttctggt ttttcgcctg ccgcgcgagt acgcacggtc
    33661 actttgtcgg ccacgataaa cgcagagtag aaaccaacac caaactgacc gatcagctgg
    33721 ctgtctttcg cttggtcaga acccagggat tcgaggaatg atttggtacc ggatttagcg
    33781 atagtcccca gatggtcaat cacttcgtcg cgggtcatcc ccacgccgtt atcggagatg
    33841 gtcagcgtgc gcttgtcttt atcgaaagag acacgaacgc gcagttcgcc atcaccttcg
    33901 tacaggtccg ggttagatag cgcacggaaa cgcagcttgt ccgccgcatc ggaggcgtta
    33961 gagataagct cacgcaggaa gatttcttta ttggaataga gagaatggat catcaggtgc
    34021 agaagctgtt tcacttctga ctgaaaacca cgagtttctt gtcctttcat gtaggtctac
    34081 ctcaataatg ccattttaag gttaaaaaca ggatgaggga gagatgggga caagggagaa
    34141 taatttcaag cgagagcagg tgctcctgct ctcgtttgct taaaaacgaa tcttatgacg
    34201 cccggcgagg gagtgtgaca gcgtggtgcc atcgaccatt tccagttcgc cgccaaccgg
    34261 tacgccatga gcgattcggc tggcttccac gtcatattgc gcgcaaagct cggcaatgta
    34321 gttagcggta gcttcacctt caaccgtggg attggtggcg aggatcactt cagtgatttt
    34381 ttcctctgcc agacgctgtt ccagacgatc aagcccgata tcatccggac cgatgccgtc
    34441 cagcggtgac agatgcccca tcaacacaaa ataacgacca gaaaactgcc ccgtctgctc
    34501 aatggcgtag atgtccgccg gactctccac cacacagatt tgaccgtttt cctgacgacg
    34561 cggattcgaa cagatgttac agacttcctg ttcggtgaaa gtgcggcaat cggcgcagtg
    34621 gccgatttcc gacatcgccc gggtgagcgc ctgcgccaga cgcatcccgc cgctacgatc
    34681 gcgctgaagc agcgtgaacg ccatacgctg cgccgacttc gggccaacgc ccggcagaca
    34741 gcgcagtgct tccataagct gtgttaacag cgggctggtt tgcatcagaa cggcatctta
    34801 aagccaggcg gcagctgcat tccggaggat acagaggcca ttttttcttt ctgcgtttct
    34861 tcaatacgac gtgctgcgtc gttgaatgct gcagccacca ggtcttccag catctctttg
    34921 tcgtcttcca gcaggctcgg gtcgatctct acgcgacggc agttgtgtgc accgttgatg
    34981 gtcactttta ccagacctgc gccagattcg ccggtaactt ccagctgcgc gatctcttcc
    35041 tgcattttct gcattttttc ttgcatctgc tgggcttgct tcatcaggtt acccagaccg
    35101 cctttaccaa acataggttt ctctctcaat cacgttaagg atgacgaacg taagctgtgc
    35161 ttacgatcaa atggggcgga tactttcttc atccagctcc gcatcgaaaa accgacgcag
    35221 ggtctgaata ttattatccg caataatgga ctcgcgcgcc tgcgcaagtt tttcttcgta
    35281 tatcgcctga cgccactcca gcggcgtacg caccgcggga ttatcatctt caacgatagt
    35341 cagttcaacc gttgaacctt ttaacatgct caacgcttca gccagttttt gctgtgcacc
    35401 gcggttgttc aaatgccgct gagaggagcg caaatgcaga catactgcgt tgtcgctctc
    35461 ctctttccag gcatttaacg ccacctgttc gaccagtttt ggtagcgaaa gttggctcac
    35521 ctgtgccgcc cacgggtcgc gctcaatggc ttctgccgct agcttcaccg ccagttccgg
    35581 cgttttttca tgttccagcg cttttttcag cgccttcggc gtggcgacca cttctttttg
    35641 ctgcatcacc ggagtggtcg ccttccagcg atacgcttct tttttggctg gcgctttttc
    35701 cagcgccgat ggcaccggac gcgcctgaac gcgatcggtg accgaagcca gtctttccag
    35761 cgcagcgtta ttcaccggcc gcgcgcgggt agcggctgcc ggttcactct tttttgcttt
    35821 ggttgctccc tgcacgcgct gcaactgctg gcgcgccgcc agcacctggc tggtggtttc
    35881 cgggagcggt acagtcggtg cctgctgcgg cgctgattgc ggcggcacca gggttggcgt
    35941 cattactgcc gttggcgcga cgggtgcaaa ggactggcgt ggcacttctg gctcaggcag
    36001 cggcatacgc ggatggaacg ccagcgcgcg cagcagcgtc atctcaacgc ccatgcggcg
    36061 gtccggcgca tacggtaatt ctttgcgacc aatcaacagc gtctgatagt aaagctgaat
    36121 atccgtcggc ggtatggtgc gcgccagttc acgcatccgc agctcgatgg cggccatgtc
    36181 gttgccaagt gcagcaggcg aaagttgtac catcgcaata cgatgcaaca agccgagcat
    36241 ttccaccagc aacgcttccc actcgatacc acgggcagcg gcttcattaa tcagtgccat
    36301 tacgcgctcg ccgttgccct cgaccatcgc ttcaaccagc gacagcgcct gatcgtcgtc
    36361 aagcgtaccc agcatcgcac tgaccgcctg ggttgaaacc tggccgtcac cgctggcaat
    36421 cgcctggtcg gtcagactta aggcatctcg caggctgcct tcagcggcgc gcgccaacaa
    36481 ttgcagcgcc cgcggctcgt gagcgatatg ttcttcattg aggatgtgct caagctgatg
    36541 gcgaatttgc tcgacatcca gcgccttgag atgaaattgc agacagcgtg acaaaatcgt
    36601 caccggcaat ttctgtggat cggtcgtcgc cagcagaaac ttaacgtgct ccggcggctc
    36661 ttcaagggtt tttaacagtg cgttaaagct gtggcgcgac agcatatgca cttcgtcgat
    36721 cagataaact ttgaaacgac cacgcgccgg agcgtactgg acgttatcca gcaggtcgcg
    36781 ggtatcttca actttggtgc gcgaggcggc gtcgatttca atcagatcga caaagcgccc
    36841 ctgctcgatt tcacgacagt tatcgcacac gccgcacggc gtcgcggtaa tgccggtttc
    36901 gcagtttagc cccttcgcca gcagtcgggc gatagaggtt tttccgacgc cacgggtgcc
    36961 ggaaaaaaga taagcatgat gaatacgccc taacgacaag ccgttcgcca gtgcggtcag
    37021 cacatgttcc tggccgacga cgtcagcaaa ggtttgtggg cgccattttc gggctaaaac
    37081 ctgataactc attggcaggc tctgaaacgc tggaaggtgg attcacgaag gggtaatgct
    37141 aacacagccc cgtcagaacg gcgaggctga atgcacatct tgtgctgtcc gtagcgtggg
    37201 cagcacaaga ctggcgataa ttaatggccc gggaacggga caaggctgta gctggtaatg
    37261 ccctgttttt cgagacgctg ttcgccgccg agatcaaaca ggttgataat gaacgcagcg
    37321 tcagccactt caccacccag acgacggatc agtttaacgg tcgcttcgat agtgccgccg
    37381 gttgccagca gatcgtccac caccagaact ttgtcgcccg gtttgatggc atcaacgtgg
    37441 atttccagct gatcggtgcc gtattccagg tcgtaatttt cactgatggt ttcacgcggc
    37501 agtttgcccg gtttacggac cggtacaaag ccaacgccca gacccagagc taccggagcg
    37561 ccaaacaaga agccacgcgc ttcggtgccg acaactttgg taatgcccgc atttttgtaa
    37621 cgctcaacca gcaagtcgat gctgagagcg taagctttcg ggtcttccag taagctggtg
    37681 acatcgcgga aaagaatgcc gggttttggg tagtcctgaa tgcttttgat gctatttttg
    37741 agatactcaa gctgctgtgc agtcgcggtc ataagtgtat gcctgcttgt tacggtggta
    37801 ctcacggcgc gttttaaacg tatcaaaagt aactgttgtt taacctttga ccgcctgcgc
    37861 ctgtgctcga aaacgccaga atgtactggc tgtacgcaat aattgcaact gcgattgttg
    37921 tgcttcagtg cttttcttgc ttttcatcaa tcaccggaat tcgccacata tagaaaagca
    37981 aacaggcgag aattaccagc aacatgatgc gcacccatgg catctggacg aaccacagag
    38041 aaatggcaaa cgtgagcaaa ataagcaaaa tcgcccgcgg tttgacgccg cgcggcatcg
    38101 catgatgttt ctgccagaaa cgtagatagc tgccaaacca tgagcggtac agcaaccagg
    38161 cgtgaaagcg cggggaagaa cgggcaaagc accaggccgc cagcaggata aacggcgtcg
    38221 tcggtaatac cggtaatacc acgcccagcg tacccagcac taccgccagc cagccaatga
    38281 tgattaaaat gattcgttgc atattgagtg ttgtcagctt acatagtggc tactttagca
    38341 taacaattat cattttcatt gaggtcttat cgtgaaaacc gccctgctgc tggaaaaact
    38401 ggaaggacag ctcgctacgc tgcgtcagcg ttgtgccccg gtgtcacagt tcgccacgct
    38461 aagtgctcgt ttcgacaggc atctttttca gactcgtgcg acaacactac agtcttgtct
    38521 cgacgaggcg ggcgataatc tggctgcgct tcgtcatgca gttgagcagc aacagctgcc
    38581 gcaagtggcc tggctggcgg aacatctggc ggcacaactg gaagccatcg cgcgtgaagc
    38641 ctccgcctgg tcattgcgcg agtgggacag tgcgccaccg aaaatttccc gctggcagcg
    38701 taaacgtatt cagcatcagg attttgagcg gcggctacgt gagatggttg ccgaacgcag
    38761 agcccgcctg gcgcgggtga ccgatctcgt ggaacagcaa acgctgcatc gtgaagtgga
    38821 agcctatgaa gcgcgcctgg cacgctgccg ccatgcgctg gaaaaaatcg aaaacaggct
    38881 agcgcgctta acccgctagc aatggaaaga gtatgtcact tgaaaatgcc cctgacgatg
    38941 tcaaactggc tgtcgatttg attgtgctgc tggaggaaaa tcagatccct gccagtaccg
    39001 tgctgcgcgc gctggatatt gtaaagcgtg attatgaaaa gaaattaacg cacgatgatg
    39061 aggcggaaaa gtgagaaata aatggggccg ctaaatgtcc cctaagattg cgggcaaacg
    39121 caaaactgcc tgatgcgcta cgcttatcag gcctacaccg ctcctgcaat atattgaatt
    39181 cttgcggttt cgtaggccgg ataaggcggt cacgccgcat ccggcatgaa caacgcgcac
    39241 tttgtcagta atctcggggc aactaaatgt cgcccccttt ggttattacc caaccgctgg
    39301 tgtcggatcg tcgcctttgt agtcgcgttt tacttccgtc acctcatcgc ccttctcgtt
    39361 gtgcagatgc acttcaagct ggttaaaggc aatgttgatg tcgttttcac ggcacagctg
    39421 atcgatagta cggttcagct catcgacagt acggctacgg tcacgcagtt cacgcacata
    39481 cagacgcagc tcatgatcca acgtgctggc accaaatgcc gtaaagaaga cttccggcat
    39541 tggttcgtgc atcacccttg ggtgctcagt cgccgccttc agtaacactt tacgcacttt
    39601 ttccagatcg gagccatagg ccacgccgag acggatcacc agacgcgtag tagtgtcagt
    39661 caacgaccag ttgatcagac gctcggtaac aaacgctttg ttcgggatga tcacttcttt
    39721 gcgatcgaaa tcggtaatcg ttgtcgcacg aatacggatc ttacttaccg tccccgagaa
    39781 gctaccaatg gttaccgtat cgccaatacg caccggacgt tcgaacagaa tgatcaaacc
    39841 ggagacgaag ttaccgaaaa tttcttgtaa accaaaacca agacctacgg ataatgctgc
    39901 ggccagccac tggagtttat cccaagagac gcccagcgat ccgaacaccg tcatcgcacc
    39961 aacggcaata atgatgtagt taaggatggt agtaatggca tacgacgcgc cctggcgcat
    40021 attcagtcgc gagagcacca gcacttccag taaaccaggc aggttgcgaa tcaacgccca
    40081 ggccaccatt gaggcgataa tcgcaaacaa cagactgccc atggtgacgt ttttcaccac
    40141 cgcagcgcca gcttcagtgc cgttgtaatg ccagagcgtg atgctgtcga gatagctgaa
    40201 cacggtgatc aaatcggacc aaattgccca gaacatgaca ccgaacagcg caaacatcag
    40261 caacatggta atacgcagcg tctgctggtt aacttgctcc agtgcaatgg tgggttcttc
    40321 cggcggttcg gcaccttctg cgccctcttt caccagattc tgccgacgcg ccagcgcacg
    40381 acgccaggcg atacgccgcg ccgctacgct taagccgcgc aataccgtct ggtagagcaa
    40441 gttccagatg atcaccagat aaacggtttc aatccaacgc ccggagaggc gcagcgtggt
    40501 gtagaaataa cccgtggcag tcagcaccat caatgcaatc gggataatcg acagcacggt
    40561 aatggtgact agtcgcatgg tgtgcgactc tttatcacgc cagctttcgc ggcacattgg
    40621 ccataccagg aaggcaatca gcagcaggtt gaagaaaatc atcgcctgcc ccagcacatc
    40681 atccatcaga tgcagcgggg aaagttctgc caccacagac cagaaatgga taggcagcaa
    40741 cgcgagactg atgcggacaa tttgccgacg ccagtggctg gtctgctgct ccggcatgcc
    40801 gaagtgacgc acggcaacgc catttttctc cagtaccttc cagcacaggc caaacaccag
    40861 ccagaaaatc gccagttttt tactgaacga ccatagcagt tcgctgatgt tgagctgcat
    40921 ggtcaacaga atcaggccaa ccgcgagaat aatcaggcac accggcagcg cgcggatcag
    40981 gtcgataagg atcgcttttg gtgtgttgag ctggctatcg ttacgcaggg aacccacagc
    41041 ggaagccagt ttctgttgat acgctttcag ccagcccaga cgccagtgga tcagcccggc
    41101 aatcaacagc agcggcaaac cggcgaggaa agcgataaaa acggcaggcc aggctttttc
    41161 ccagttcacc gtgattttca tcgacttaaa ttcatctttc aggctttgcg ggaacgcttt
    41221 gatccagtcc cagtccattg gacggttact gttcacccaa aagatttgct gagtcaggat
    41281 ggatttcagg tttttcgata cgctcattaa ctgctgctgg ttgatttgca ggttaatggc
    41341 catcatcagc tggttaccca actgtttgtt gagttgatcc agcaattcgc gacgcatatc
    41401 aaccacttgc aataacgcat cgtgaacttc gctgttgact tcgttggtgt gaccttcttc
    41461 cagtttgttg acgaacgcat cgctctggaa gagtgcatca cgctgctggt taacttcaaa
    41521 ctgttcgaga cgcaaatccg cgatgcggtt ggtcatgttt tccagttcat ccgccgaggg
    41581 cagcgtttgt tgttgctggt aaaggatacg agacaacagc aggctgccct tcaggacggc
    41641 aatctgctct ttaatattgc gttccgattg cagcgcccgc tccagccagt ttttgacttt
    41701 aatgttttgc tgcatcaact gattaccgtt ttcagtcgcg gtaatcagac gctgacttaa
    41761 ctgctggtta atttccagtt cctgtttcac cagcggatta gcctgaatac gcgcggcttc
    41821 atccggggag acggcttcct gcgccgtttt ttcagttaaa gtcaggcgct tgctgtttac
    41881 cgcttcttgc aacagctgca actggtgctc cagacgggcg ctgttcgccg tcacgtaatc
    41941 acgttgcttt tgcaaggtat cctgcaagac ggtgttccct tccaggcttt tacgctgctg
    42001 gtcaatctcc gcattcagca acgcctgctg agcctgcatt aacactttct ggctgggacg
    42061 taaggcagtc tcgccgacat cagtcccatc cagacgactg cgaatttgtt gcagctgctg
    42121 cgaagcgtta tacatcgcat tttgcacgcg ttcgggctgc gtctgtaacg aaaccagctg
    42181 gctgttataa gacgccagat cgttttgtgc gttttgcaaa tcgtccagcg cctgggcaac
    42241 gcgagtttcc agctggcgca acgaaagcgt actcagaatt ttgcgcgttt cttcgtcgtt
    42301 atcgacatcg ctaagtgctg ttaacgccgc ggtcgcctgg cgcatttttt ccggcgcttc
    42361 agcgactttt tgccgtagct gaactgtctc ttcttttatg cgatcgattt tatcgagggt
    42421 ggctaatgta tctgtcagat cctgctgcac cagtttgtcc tgagcagaaa gatctttttg
    42481 tttatttagt gagtcaagtt gcgcctgcag gtccgctttt gtcggcagat caccattcga
    42541 tgacgcccgc gcaaacgccg tgttctggca taacaagaca aaaacaaaag caataaatgc
    42601 tgaaaaaaca aaatgccgtg atcgtttgta atactggaac atagtcatga tgaatgaagg
    42661 tttctgaacc tgaagaacga cctgaaaaag tcaaaccgca agaatatcac gacgcagtga
    42721 accagaatag caacgacgaa aatgtccagg aaaaatcctg gagtcagatt cagggttatt
    42781 cgttagtggc aggattacga agcgtggggc acaggagata catctccagt aagatggcga
    42841 cgtaatcgcg ggcttctttt ttaagatcaa aagattgcgg ggcaaagagc cagttttcca
    42901 tcaggccgga aatatagccg cgcataataa ttgctgcccg acgcgtcatt aaatccgcag
    42961 gcaacatttt cgcttcaata caatgtttta acgtttgttc tatacggtca taactttcca
    43021 gacagagatt acgttgtgcc tgttgcacaa cagccatttc tccgacaaat tcgcatttgt
    43081 ggaatataat ctccatcaat aatcgacgtc gttcttctgt caccgtggat tcaagaacat
    43141 gaattaatat ctctcttaat actgagagtg gatcgccagg gaattttgcc tgatactcaa
    43201 gctctagttc accaatattg gattctgaca gttcccagat ctcactgaac aaatccgact
    43261 tgtctttaaa atgccagtag attgcaccgc gcgtaacgcc agctgctttt gcaatctcgc
    43321 ccagcgaggt ggatgatacc ccctgctgtg agaaaagacg tagagccaca tcgaggatgt
    43381 gttggcgcgt ttcttgcgct tcttgtttgg tttttcgtgc catatgttcg tgaatttaca
    43441 ggcgttagat ttacatacat ttgtgaatgt atgtaccata gcacgacgat aatataaacg
    43501 cagcaatggg tttattaact tttgaccatt gaccaatttg aaatcggaca ctcgaggttt
    43561 acatatgaac aaaaacagag ggtttacgcc tctggcggtc gttctgatgc tctcaggcag
    43621 cttagcccta acaggatgtg acgacaaaca ggcccaacaa ggtggccagc agatgcccgc
    43681 cgttggcgta gtaacagtca aaactgaacc tctgcagatc acaaccgagc ttccaggtcg
    43741 caccagtgcc taccggatcg cagaagttcg tcctcaagtt agcgggatta tcctgaagcg
    43801 taatttcaaa gaaggtagcg acatcgaagc tggtgtctct ctctatcaga ttgatcctgc
    43861 gacctatcag gcgacatacg acagtgcgaa aggtgatctg gcgaaagccc aggctgcagc
    43921 caatatcgcg caattgacgg tgaatcgtta tcagaaactg ctcggtactc agtacatcag
    43981 taagcaaggg tacgatcagg ctctggctga tgcgcaacag gcgaatgctg cggtaactgc
    44041 ggcgaaagct gccgttgaaa ctgcgcggat caatctggct tacaccaaag tcacctctcc
    44101 gattagcggt cgcattggta agtcgaacgt gacggaaggc gcattggtac agaacggtca
    44161 ggcgactgtg ctggcaaccg tgcagcaact tgatccgatc tacgttgatg tgacccagtc
    44221 cagcaacgac ttccttcgcc tgaaacagga actggcgaat ggcacgctga aacaagagaa
    44281 cggcaaagcc aaagtgtcgc tgatcaccag tgacggcatt aagttcccgc aggacggtac
    44341 gctggaattc tctgacgtta ccgttgatca gaccactggg tctatcaccc tacgcgctat
    44401 cttcccgaac ccggatcaca ctctgctgcc gggtatgttc gtgcgcgcac gtctggaaga
    44461 agggcttaat ccaaacgcta ttttagtccc gcaacagggc gtaacccgta cgccgcgtgg
    44521 tgatgccacc gtactggtag ttggcgcgga tgacaaagtg gaaacccgtc cgatcgttgc
    44581 aagccaggct attggcgata agtggctggt gacagaaggt ctgaaagcag gcgatcgcgt
    44641 agtaataagt gggctgcaga aagtgcgtcc tggtgtccag gtaaaagcac aagaagttac
    44701 cgctgataat aaccagcaag ccgcaagcgg tgctcagcct gaacagtcca agtcttaact
    44761 taaacaggag ccgttaagac atgcctaatt tctttatcga tcgcccgatt tttgcgtggg
    44821 tgatcgccat tatcatcatg ttggcagggg ggctggcgat cctcaaactg ccggtggcgc
    44881 aatatcctac gattgcaccg ccggcagtaa cgatctccgc ctcctacccc ggcgctgatg
    44941 cgaaaacagt gcaggacacg gtgacacagg ttatcgaaca gaatatgaac ggtatcgata
    45001 acctgatgta catgtcctct aacagtgact ccacgggtac cgtgcagatc accctgacct
    45061 ttgagtctgg tactgatgcg gatatcgcgc aggttcaggt gcagaacaaa ctgcagctgg
    45121 cgatgccgtt gctgccgcaa gaagttcagc agcaaggggt gagcgttgag aaatcatcca
    45181 gcagcttcct gatggttgtc ggcgttatca acaccgatgg caccatgacg caggaggata
    45241 tctctgacta cgtagcggcg aatatgaaag atgccatcag ccgtacgtcg ggcgtgggtg
    45301 atgttcagtt gttcggttca cagtacgcga tgcgtatctg gatgaacccg aatgagctga
    45361 acaaattcca gctaacgccg gttgatgtca ttaccgccat caaagcgcag aacgcccagg
    45421 ttgcggcggg tcagctcggt ggtacgccgc cggtgaaagg ccaacagctt aacgcctcta
    45481 ttattgctca gacgcgtctg acctctactg aagagttcgg caaaatcctg ctgaaagtga
    45541 atcaggatgg ttcccgcgta ctgctgcgtg atgtggcgaa gattgagctg ggtggtgaga
    45601 actacgacat catcgcagag tttaacggcc aacctgcttc cggtctgggg atcaagctgg
    45661 cgaccggtgc aaacgcgctg gataccgctg cggcaatccg tgctgaactg gcgaagatgg
    45721 aaccgttctt cccgtcgggt ctgaaaattg tttacccgta cgacaccacg ccgttcgtga
    45781 aaatctctat tcacgaagtg gttaaaacgc tggtcgaagc gatcatcctc gtgttcctgg
    45841 ttatgtatct gttcctgcag aacttccgcg cgacgttgat tccgaccatt gccgtaccgg
    45901 tggtattgct cgggaccttt gccgtccttg ccgcctttgg cttctcgata aacacgctaa
    45961 caatgttcgg gatggtgctc gccatcggct tgttggtgga tgacgctatc gttgtggtag
    46021 aaaacgtgga gcgtgttatg gcggaagaag gtttgccgcc aaaagaagct acccgtaagt
    46081 cgatggggca gattcagggc gctctggtcg gtatcgcgat ggtactgtcg gcggtattcg
    46141 taccgatggc cttctttggc ggttctactg gtgctatcta tcgtcagttc tctattacca
    46201 ttgtttcagc aatggcgctg tcggtactgg tagcgttgat cctgactccg gctctttgtg
    46261 ccaccatgct gaaaccgatt gccaaaggcg atcacgggga aggtaaaaaa ggcttcttcg
    46321 gctggttgaa ccgcatgttc gagaagagca cgcaccacta caccgacagc gtaggcggta
    46381 ttctgcgcag tacggggcgt tacctagtgc tgtatctgat catcgtggtc ggcatggcct
    46441 atctgttcgt gcgtctgcca agctccttct tgccagatga ggaccagggc gtatttatga
    46501 ccatggttca gctgccagca ggtgcaacgc aggaacgtac gcagaaagtg ctcaatgagg
    46561 taacgcatta ctatctgacc aaagaaaaga acaacgttga gtcggtgttc gccgttaacg
    46621 gcttcggctt tgcgggacgt ggtcagaata ccggtattgc gttcgtttcc ttgaaggact
    46681 gggccgatcg tccgggcgaa gaaaacaaag ttgaagcgat taccatgcgt gcaacacgcg
    46741 ctttctcgca aatcaaagat gcgatggttt tcgcctttaa cctgcccgca atcgtggaac
    46801 tgggtactgc aaccggcttt gactttgagc tgattgacca ggctggcctt ggtcacgaaa
    46861 aactgactca ggcgcgtaac cagctgcttg cagaagcagc gaagcaccct gatatgctga
    46921 ccagcgtacg tccaaacggt ctggaagata ccccgcagtt taagattgat atcgaccagg
    46981 aaaaagcgca ggcgctgggt gtttctatca acgacattaa caccactctg ggcgctgcat
    47041 ggggcggtag ctatgtgaac gactttatcg accgcggtcg tgtgaagaaa gtttacgtca
    47101 tgtcagaagc gaaataccgt atgctgccgg atgatatcgg cgactggtat gttcgtgctg
    47161 ctgatggtca gatggtgcca ttctcggcgt tctcctcttc tcgttgggag tacggttcgc
    47221 cgcgtctgga acgttacaac ggcctgccat ccatggaaat cttaggccag gcggcaccgg
    47281 gtaaaagtac cggtgaagca atggagctga tggaacaact ggcgagcaaa ctgcctaccg
    47341 gtgttggcta tgactggacg gggatgtcct atcaggaacg tctctccggc aaccaggcac
    47401 cttcactgta cgcgatttcg ttgattgtcg tattcctgtg tctggcggcg ctgtacgaga
    47461 gctggtcgac tccgttctcc gttatgctgg tcgttccgct gggggttatc ggtgcgttgc
    47521 tggctgccac cttccgtggc ctgaccaatg acgtttactt ccaagtagga ctgctcacaa
    47581 ccattgggtt gtcggcgaag aacgcgatac ttatcgtcga attcgccaaa gacttgatgg
    47641 ataaagaagg taaaggtctg attgaagcga cgcttgatgc ggtgcggatg cgtttacgtc
    47701 cgatcctgat gacctcgctg gcgtttatcc tcggcgttat gccgctggtt atcagtactg
    47761 gtgctggttc cggcgcgcag aacgcagtag gtaccggtgt aatgggcggg atggtgaccg
    47821 caacggtact ggcaatcttc ttcgttccgg tattctttgt ggtggttcgc cgccgcttta
    47881 gccgcaagaa tgaagatatc gagcacagcc atactgtcga tcatcattga tacaacatgt
    47941 aatcactaag gccgcgtaag cggccttttt tatgcataac ctacgaacat taaggagtaa
    48001 ttgaaccacc aactcaggat ctcatacgaa aaccagtatt aaccacgtat aaaattcata
    48061 aaaaatactg attgttagtt aatttatatt aagtagcgct aatagattta ataatccata
    48121 atcatttaga ggctattctt aattatttgc ggtaattctt tattcattcc tcggttatta
    48181 cgtcatattc agagcaatcc tggtattagt gtcaccaatt tcatctggcg ataatcctga
    48241 aatgttatga acagttcgag caaactgctt ttacctgctg cgggttagtg ctagtatgaa
    48301 aaagtgagtc ctgtcccgat tccttcctaa ttgtaatgtt tcgtaataat gcgatgaaaa
    48361 cctgcaaaga gtggcttata gttaagctaa caaacgagag ggcaagtcta ggtcagtaag
    48421 ttttttccat cccgaaaggt gtccgttagt tcaaccacta agaaggggac gcgttatgga
    48481 tgaatactca cccaaaagac atgatatcgc acagcttaag tttctctgtg aaaccctgta
    48541 tcatgactgt cttgcaaacc ttgaagaaag caatcatggt tgggttaacg acccaacctc
    48601 ggcgatcaac ctccagttga atgaactgat tgagcatatt gcgaccttcg cacttaatta
    48661 caaaattaag tataatgaag acaataagct cattgagcag atcgacgaat atctggatga
    48721 cacctttatg ttgttcagca gttatggtat taatatgcag gctcttcaga aatggcggaa
    48781 gtcaggtaat cgactattcc gttgttttgt caacgcgacg aaagagaatc ctgcgagttt
    48841 atcttgttag aattattaca accatgggta gaagtatgtc cgaaaaacct ttaacaaaaa
    48901 ccgattattt aatgcgttta cgtcgttgcc agacaattga cacgctggag cgtgttatcg
    48961 agaaaaataa atacgaatta tcagataatg aactggcggt attttactca gccgcagatc
    49021 accgcctcgc cgaattgacc atgaataaac tgtacgacaa gatcccttcc tcagtatgga
    49081 aatttattcg ctaataaata attcgctttc ggtgctataa ccggctgttt attaagaatt
    49141 ttatactttt tcgccatgaa gacataccct atgtgatctt tatcacacag atgtaatgag
    49201 aacgttctct tcactgactt ttcgtcttac tgtgttgccg cattttcagc aaccggaggt
    49261 cagtaatgag cacagaaaaa gaaaagatga ttgctggtga gttgtatcgc tcggcagatg
    49321 agacgttatc tcgcgatcgc ctgcgcgctc gtcagcttat tcaccgatac aatcattccc
    49381 tggcggaaga gcacacatta cgccagtaaa ttctcgctaa tttattcggt caggtgacag
    49441 aggcttatat tgagccaacg tttcgctgtg attatggcta taacattttt ctcggcaata
    49501 attttttcgc caacttcgat tgcgtgatgc ttgatgtctg ccctattcgc atcggtgata
    49561 actgtatgtt ggcaccaggc gttcatatct acacggcaac acatcccatc gaccctgtag
    49621 cacgtaatag cggtgctgaa ctggggaaac ccgtcaccat cggtaataac gtctggattg
    49681 gcggacgcgc ggtcattaac ccttgtgtga ccattggtga taacgtcgtg gtagcttcag
    49741 gtgcagttgt cacaaaaggt gtcccggaca acgttgtcgt gggcggtaat ccagccagaa
    49801 taattaaaaa attgtaatcg gtttttcgca actgttatac aaaattgtgg tagatctgtt
    49861 acttcccctc tactattccc acgttaaaat agggtgttcc ctggaaagtt gcagatacca
    49921 cgaaggcaaa cgatgaccga aatacaacgc ctgctgaccg aaacgattga gtctctgaat
    49981 acccgcgaaa aacgcgacaa caaaccccgc tttagtatca gttttatccg taaacatccg
    50041 gggctgttta tcggtatgta cgttgctttt tttgccaccc tggcggtgat gttgcagtcc
    50101 gaaacgctgt caggctctgt ctggctactg gttgtattat ttatcctgct taatggtttc
    50161 ttctttttcg atgtctaccc acgctaccgc tatgaagata tcgacgtgct ggatttccgc
    50221 gtttgctata acggcgaatg gtacaacacg cgctttgtac ctgccgcgct ggttgaagcc
    50281 atcttgaact ctccgcgtgt cgcggatgtt cataaggaac aactgcaaaa aatgatcgtc
    50341 cgtaaaggtg aactgtcttt ttacgatatt tttaccctcg ctcgcgccga atcaacatct
    50401 taagttaggg ttacatacca ggcgtaaagc tctgcgcctg gtcaaatgac aatgatcgtt
    50461 tccacccatc acttcatgaa ataccagctc tacctcctta tctcctgcca gcctttttcc
    50521 acaatcagat atactttccc tacactgtgt taataaggat atgctggtga gaacacgaca
    50581 tctggtcggc cttatttcgg gagtactgat tctttcagta ttgctgcctg tcggcttaag
    50641 catctggctg gcccatcagc aggtagaaac atcgtttatt gaagagctgg atacctattc
    50701 ctcccgcgtc gctattcgag ccaataaggt ggcgacacaa gggaaagatg cgctgcagga
    50761 gctggaaaga tggcaaggcg ctgcctgtag cgaagcccat ctcatggaaa tgcgtcgggt
    50821 atcttacagt tatcgctata ttcaggaagt ggtttatatc gataacaacg ttccccagtg
    50881 ttcgtctctg gagcatgaaa gtccgcccga tacctttccc gagctaggta aaatttcgaa
    50941 agatggttat cgtgtctggt taacatcgca taacgattta ggcattatcc gttacatggt
    51001 cgccatggga acggcacatt atgtcgtcat gatcgacccc gcttccttta ttgatgtcat
    51061 tccctatagt tcatggcaaa ttgatgccgc cattattggc aatgcccata acgttgtcat
    51121 aaccagcagc gatgaaattg ctcagggaat tattaccagg ctacaaaaaa cacccggtga
    51181 gcatatcgaa aataatggga tcatttacga tatactgccc ttcctggaga tgaatatttc
    51241 gatcatcacc tgggcttcaa cgaaaatgtt gcagaaaggc tggcatcggc aagtctttat
    51301 ttggttaccg ctcgggttgg tgattggcct gctggcagcg atgtttgtgc tgcgtatttt
    51361 gcgccgtatc caatcaccgc atcatcggct gcaggatgct atcgaaaatc gtgatatttg
    51421 cgtgcactat cagccgattg tctccttagc caatggcaaa attgtcggtg ctgaggcact
    51481 ggcgcgctgg ccgcagacag acggtagttg gttgtcacca gatagtttta ttccgctggc
    51541 acagcaaacg ggcctttctg agccattgac gctactgatt ataagaagcg tctttgaaga
    51601 tatgggcgac tgcttgcgtc agcatccaca gcagcatatt tcgatcaatc ttgaatccac
    51661 cgtgctcacc tcggaaaaaa tcccgcaatt gctgcgtgaa atgatcaatc actatcaggt
    51721 taatcccaga cagatcgcgc ttgaactcac tgaacgcgag tttgccgatc cgaaaaccag
    51781 cgccccgata atttctcgct accgggaggc gggccatgaa atttatcttg atgattttgg
    51841 tacggggtat tcaagtttaa gttatttaca ggatctggat gtcgacattc tgaagatcga
    51901 taaatctttc gttgatgcgc tggaatataa aaatgtcacg ccgcatatca tcgaaatggc
    51961 aaaaacactg aaactgaaaa tggtagcgga gggaatcgaa accagtaaac aagaagagtg
    52021 gctacgccag catggcgtgc actacggtca gggctggctc tacagcaagg cattaccgaa
    52081 agaagatttc ttacgctggg ccgagcaaca tttgtgaatt tttgggctga cggcacgccg
    52141 cacagcccgt cacgctacct attcgtcgat gattgactcg aatccgccat agatcattcg
    52201 cttgccgtca aacggcatgg actcgccgaa ctctttcatc cgtgggtccg acatcatctt
    52261 ttgattagct gcgtcgcgga cctcttttga agggtattca atccagctaa agacaacctc
    52321 ttcattctct tccgctttca ccgccatacg aaaatcggtc actttgccat ccggcacatc
    52381 gctggcccag cattcgacaa tacgaagcgc gccaaactct ttaaacaatg gcgcggcctt
    52441 agcggccatt tcccgatagg catccttttt atcggcagga acggcaacca caaaaccatc
    52501 aacatacttc atcagacata cctccgaagc gtactgcgca gccagacaat tcttgcgacg
    52561 tcagttgtgg ttaaagttta gccatgctgg gattttttga cgaaacggtg ggaggcagaa
    52621 atgggtgggg gccctgccag ctacatcccg gcacacgcgt catctgcctt ggctgcttcc
    52681 ttccggacct gacctggtaa acagagtagc attgcgggag aaccaacaga gcccccattg
    52741 agagcgttga aaaccaacgc gcaggcgcat tatcactgct gccccggcta attgcaaccc
    52801 attgcgtgcc agatgctggt ttcttgcgca atgcgacttc actcgggcgt ttttccagat
    52861 tatgctgaaa aattacctca ggaagaaaag atggaaaaag aagattcatt tccccaacgc
    52921 gtctggcaaa tcgtcgccgc tattcccgaa ggctatgtca ccacttacgg tgatgtggcg
    52981 aaactggcgg gatcgccccg cgccgcgcgc caggtgggcg gtgtgttaaa gcgtctccct
    53041 gaaggcagca ccttaccctg gcaccgggtg gttaatcgcc atggcacaat ttcgctaacc
    53101 gggccggatt tacagcgtca gcgacaggca ttactggcag aaggtgtgat ggtatcggga
    53161 agcgggcaaa tcgacttgca gcgttatcgc tggaactact aaccctctcc gg
//
LOCUS       DAJTRD010000020        49818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTRD010000020 DAJTRD010000000
VERSION     DAJTRD010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.020"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..1222
                     /locus_tag="OO315_001279"
     CDS             <1..1222
                     /locus_tag="OO315_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5890260.1"
                     /translation="ELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENHP
                     VITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMII
                     PEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNIA
                     LLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVYV
                     NTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKNF
                     KVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPETV
                     LNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEKY
                     LLAQFGVDIGEAAQ"
     gene            1219..2184
                     /gene="yggF"
                     /locus_tag="OO315_001280"
     CDS             1219..2184
                     /gene="yggF"
                     /locus_tag="OO315_001280"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5890261.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2206..2715
                     /gene="fumE"
                     /locus_tag="OO315_001281"
     CDS             2206..2715
                     /gene="fumE"
                     /locus_tag="OO315_001281"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5890262.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2712..3425
                     /locus_tag="OO315_001282"
     CDS             2712..3425
                     /locus_tag="OO315_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5890263.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLVALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3397..4074)
                     /locus_tag="OO315_001283"
     CDS             complement(3397..4074)
                     /locus_tag="OO315_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5890264.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4068..4745)
                     /locus_tag="OO315_001284"
     CDS             complement(4068..4745)
                     /locus_tag="OO315_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5890265.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4733..5116)
                     /locus_tag="OO315_001285"
     CDS             complement(4733..5116)
                     /locus_tag="OO315_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5890266.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5132..5440)
                     /locus_tag="OO315_001286"
     CDS             complement(5132..5440)
                     /locus_tag="OO315_001286"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890267.1"
                     /translation="MHPLTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5441..6019)
                     /locus_tag="OO315_001287"
     CDS             complement(5441..6019)
                     /locus_tag="OO315_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5890268.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6042..6473)
                     /locus_tag="OO315_001288"
                     /pseudo
     CDS             complement(6042..6473)
                     /locus_tag="OO315_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6845..7864
                     /gene="epd"
                     /locus_tag="OO315_001289"
     CDS             6845..7864
                     /gene="epd"
                     /locus_tag="OO315_001289"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5890269.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7914..9077
                     /gene="pgk"
                     /locus_tag="OO315_001290"
     CDS             7914..9077
                     /gene="pgk"
                     /locus_tag="OO315_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5890270.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9292..10371
                     /gene="fbaA"
                     /locus_tag="OO315_001291"
     CDS             9292..10371
                     /gene="fbaA"
                     /locus_tag="OO315_001291"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5890271.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10562..11422
                     /gene="mscS"
                     /locus_tag="OO315_001292"
     CDS             10562..11422
                     /gene="mscS"
                     /locus_tag="OO315_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5890272.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11561..12196
                     /gene="argO"
                     /locus_tag="OO315_001293"
     CDS             11561..12196
                     /gene="argO"
                     /locus_tag="OO315_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5890273.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12289..13029
                     /locus_tag="OO315_001294"
     CDS             12289..13029
                     /locus_tag="OO315_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5890274.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13196..14146
                     /locus_tag="OO315_001295"
     CDS             13196..14146
                     /locus_tag="OO315_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5890275.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14088..15566)
                     /gene="scpC"
                     /locus_tag="OO315_001296"
                     /pseudo
     CDS             complement(14088..15566)
                     /gene="scpC"
                     /locus_tag="OO315_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15590..16375)
                     /gene="scpB"
                     /locus_tag="OO315_001297"
     CDS             complement(15590..16375)
                     /gene="scpB"
                     /locus_tag="OO315_001297"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5890276.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16386..17381)
                     /gene="meaB"
                     /locus_tag="OO315_001298"
     CDS             complement(16386..17381)
                     /gene="meaB"
                     /locus_tag="OO315_001298"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5890277.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17374..19518)
                     /gene="scpA"
                     /locus_tag="OO315_001299"
     CDS             complement(17374..19518)
                     /gene="scpA"
                     /locus_tag="OO315_001299"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5890278.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19722..20615)
                     /gene="argP"
                     /locus_tag="OO315_001300"
     CDS             complement(19722..20615)
                     /gene="argP"
                     /locus_tag="OO315_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5890279.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            20998..21657
                     /gene="rpiA"
                     /locus_tag="OO315_001301"
     CDS             20998..21657
                     /gene="rpiA"
                     /locus_tag="OO315_001301"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5890280.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21913..23145
                     /gene="serA"
                     /locus_tag="OO315_001302"
     CDS             21913..23145
                     /gene="serA"
                     /locus_tag="OO315_001302"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5890281.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23374..23433
                     /gene="ibsC"
                     /locus_tag="OO315_001303"
     CDS             23374..23433
                     /gene="ibsC"
                     /locus_tag="OO315_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5890282.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23766..23825
                     /locus_tag="OO315_001304"
     CDS             23766..23825
                     /locus_tag="OO315_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5890283.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23926..24528)
                     /locus_tag="OO315_001305"
     CDS             complement(23926..24528)
                     /locus_tag="OO315_001305"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5890284.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24549..24732)
                     /gene="ssrS"
                     /locus_tag="OO315_001306"
     ncRNA           complement(24549..24732)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO315_001306"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24774..25103)
                     /gene="zapA"
                     /locus_tag="OO315_001307"
     CDS             complement(24774..25103)
                     /gene="zapA"
                     /locus_tag="OO315_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5890285.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25271..25849
                     /gene="ygfB"
                     /locus_tag="OO315_001308"
     CDS             25271..25849
                     /gene="ygfB"
                     /locus_tag="OO315_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5890286.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25875..27200
                     /gene="pepP"
                     /locus_tag="OO315_001309"
     CDS             25875..27200
                     /gene="pepP"
                     /locus_tag="OO315_001309"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5890287.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27197..28375
                     /gene="ubiH"
                     /locus_tag="OO315_001310"
                     /gene_synonym="visB"
     CDS             27197..28375
                     /gene="ubiH"
                     /locus_tag="OO315_001310"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5890288.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28398..29600
                     /gene="ubiI"
                     /locus_tag="OO315_001311"
                     /gene_synonym="visC"
     CDS             28398..29600
                     /gene="ubiI"
                     /locus_tag="OO315_001311"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5890289.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29674..29757)
                     /locus_tag="OO315_001312"
     CDS             complement(29674..29757)
                     /locus_tag="OO315_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890290.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30048..31142
                     /gene="gcvT"
                     /locus_tag="OO315_001313"
     CDS             30048..31142
                     /gene="gcvT"
                     /locus_tag="OO315_001313"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5890291.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31166..31555
                     /gene="gcvH"
                     /locus_tag="OO315_001314"
     CDS             31166..31555
                     /gene="gcvH"
                     /locus_tag="OO315_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5890292.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31674..34547
                     /gene="gcvP"
                     /locus_tag="OO315_001315"
     CDS             31674..34547
                     /gene="gcvP"
                     /locus_tag="OO315_001315"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5890293.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34814..35557
                     /locus_tag="OO315_001316"
     CDS             34814..35557
                     /locus_tag="OO315_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5890294.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35614..37047)
                     /gene="bglA"
                     /locus_tag="OO315_001317"
     CDS             complement(35614..37047)
                     /gene="bglA"
                     /locus_tag="OO315_001317"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5890295.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37092..37402
                     /locus_tag="OO315_001318"
                     /pseudo
     CDS             37092..37402
                     /locus_tag="OO315_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37566..38225
                     /locus_tag="OO315_001319"
     CDS             37566..38225
                     /locus_tag="OO315_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5890296.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38421..39401)
                     /gene="ygfZ"
                     /locus_tag="OO315_001320"
     CDS             complement(38421..39401)
                     /gene="ygfZ"
                     /locus_tag="OO315_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5890297.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39644..39910
                     /gene="sdhE"
                     /locus_tag="OO315_001321"
     CDS             39644..39910
                     /gene="sdhE"
                     /locus_tag="OO315_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5890298.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39891..40298
                     /locus_tag="OO315_001322"
     CDS             39891..40298
                     /locus_tag="OO315_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5890299.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40338..40859)
                     /gene="fldB"
                     /locus_tag="OO315_001323"
     CDS             complement(40338..40859)
                     /gene="fldB"
                     /locus_tag="OO315_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5890300.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40971..41867
                     /gene="xerD"
                     /locus_tag="OO315_001324"
     CDS             40971..41867
                     /gene="xerD"
                     /locus_tag="OO315_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5890301.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41892..42602
                     /gene="dsbC"
                     /locus_tag="OO315_001325"
     CDS             41892..42602
                     /gene="dsbC"
                     /locus_tag="OO315_001325"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5890302.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42608..44341
                     /gene="recJ"
                     /locus_tag="OO315_001326"
     CDS             42608..44341
                     /gene="recJ"
                     /locus_tag="OO315_001326"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5890303.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44432..45530
                     /gene="prfB"
                     /locus_tag="OO315_001327"
     CDS             join(44432..44506,44508..45530)
                     /gene="prfB"
                     /locus_tag="OO315_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5890304.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45540..47057
                     /gene="lysS"
                     /locus_tag="OO315_001328"
     CDS             45540..47057
                     /gene="lysS"
                     /locus_tag="OO315_001328"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5890305.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47100..47648)
                     /gene="idi"
                     /locus_tag="OO315_001329"
     CDS             complement(47100..47648)
                     /gene="idi"
                     /locus_tag="OO315_001329"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5890306.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47703..47774
                     /locus_tag="OO315_001330"
     CDS             47703..47774
                     /locus_tag="OO315_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890307.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47771..47896)
                     /locus_tag="OO315_001331"
     CDS             complement(47771..47896)
                     /locus_tag="OO315_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890308.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47898..49346)
                     /gene="uacT"
                     /locus_tag="OO315_001332"
     CDS             complement(47898..49346)
                     /gene="uacT"
                     /locus_tag="OO315_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5890309.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        12048 a        12449 c        13405 g        11916 t
ORIGIN      
        1 tgaactgcca gaaattaccg ataatgaact gttagtgagt gtaatttctg acagcgtctg
       61 tttatcgacc tggaaagcgg cgttactcgg tagtgaacat aaacgcgtac ccgacgattt
      121 agaaaatcat ccggtcatta ccgggcatga atgtgccggg gttattgtcg aagtgggtaa
      181 aaatctcact ggcaaatata aaaaaggcca gcgttttgta ttgcaaccgg cgatggggtt
      241 accaagcgga tattcagcgg gctacagcta cgaatatttt ggcggcaatg ccacttatat
      301 gattattccc gaaatcgcca ttaatttggg ctgcgtatta ccgtatcacg gctcttattt
      361 tgctgcggcg tcgctggcag agcctatgtg ctgcattatt ggtgcttatc atgccaatta
      421 tcacaccacg caatatgttt atgagcatcg catgggcgtc aaacctggcg gcaatattgc
      481 actgctggcg tgtgcgggtc cgatgggcat tggcgctatc gattacgcca ttaacggtgg
      541 catacaaccg tcacgggtgg tggtggtcga tatcgacgac aaacgcctgg cgcaggtgca
      601 gaagctgctg ccggtggatc tggcggccag taaaggcatt gagctggtgt atgtgaatac
      661 caaagggatg agcgatccta tccagatgct gcgggcgctg acaggagatg tcgggttcga
      721 tgacattttt gtttatgcgg cggtgcctgc tgtcgttgag atggctgacg aattactggc
      781 ggaagatggc tgtctgaact tctttgccgg gctgacggat aaaaacttca aagtgccgtt
      841 taatttctac aacgtccatt acaacagcac gcacgtagtc ggtacatccg gcggttcaac
      901 ggacgacatg aaagaggcga ttgcccttag cgccacagga cagttacagc cgtcgtttat
      961 ggtgacccat attggtggcc tggatgcggt gccagaaacc gtgctcaatc tgccggatat
     1021 ccctggcggt aaaaaactca tttataacgg cgtgaccatg ccgctcactg ccattgccga
     1081 ttttgccgaa aaaggcaaaa ccgatccgct gtttaaagag ttggcgcggc tggttgagga
     1141 aacgcacggc atctggaatg aacaggccga gaaatatctg ctggcacaat ttggcgttga
     1201 tatcggggag gccgcgcaat gatgtccctg gcgtggccat tatttcgcgt tacggaacag
     1261 gcagcgttgg ctgcctggcc gcaaaccgga tgtggcgaca aaaataaaat tgatggcctg
     1321 gcggtcaccg cgatgcgcca ggcattaaac gacgtcgctt ttcgtggacg agtggttatc
     1381 ggcgaaggag aaattgacca tgcgccgatg ctgtggattg gcgaagaggt gggcaaaggc
     1441 gatgggccag aagtcgatat cgcggttgat cccattgaag gcacgcggat ggtggcgatg
     1501 gggcagagca atgcgctggc ggtcatggct ttcgctcctc gagatagcct gttacatgcg
     1561 cccgatatgt atatgaaaaa gctggttgtt aatcgactgg ctgccggggc gattgatctg
     1621 tctttgcctc tggcggacaa cttgcgtaac gtggcgcggg cgttaggtaa accgctggac
     1681 aagctgcgca tggttacgct ggataaaccg cgtctgagtg ccgcgattga agaagcaaca
     1741 cagctgggcg tgaaggtttt tgccctgccg gatggcgatg tcgccgccag cgtgctgacc
     1801 tgctggcagg ataatcctta cgatgtgatg tacaccatcg gcggtgcgcc agaaggcgtg
     1861 atttctgcct gtgccgttaa agcgttaggc ggggatatgc aggccgagct gattgacttt
     1921 tgtcaggcta aaggggatta cacggaaaat cggcagattg cagagcagga gcgcaagcgt
     1981 tgtaaggcaa tgggcgttga cgttaaccgt gtgtactcgc tcgatgaact ggtgaggggg
     2041 aacgatatcc tctttagcgc tacgggcgtg acgggcggcg agttggtgaa cggtatccaa
     2101 cagacggcga atggggtgcg gacgcagaca ttactgatcg gcggcgcgga ccaaacgtgt
     2161 aatataatag actccctgca ttgatggtga tctatcggaa gaccaatggc gacgctgaca
     2221 gaagatgatg tgcttgagca actggatgca caggacaatt tattttcatt tatgaaaact
     2281 gcgcattcta ttttgctcca ggggatacgc cagtttctgc cgtcgctgtt tgtcgataac
     2341 gatgaagaga tcgtcgaata tgcagtgaag ccgttactcg cccaaagcgg cccgcttgac
     2401 gatattgatg ttgcgctgcg tttgatttat gcgctgggga aaatggataa atggctgtat
     2461 gccgatatca cgcacttttc ccagtactgg cattacctga acgaacagga tgaaacgccc
     2521 ggatttgccg atgatataac ctgggatttt atcagcaatg tcaacagtat aactcgcaat
     2581 gcgacgctct acgatgcgtt aaaagcgatg aagttcgccg atttcgccgt ctggtcagag
     2641 gcgcgtttta gcggaatggt caaaacggcg ctgacgctgg cagtaacgac caccttaaag
     2701 gaattaacgc cgtgaaaatt gaattaacgg tgaatgggct gaacgtgcaa gcgcagtacc
     2761 atgatgaaga gatagaacgc gtgcataaac tgttgctgcg tatgctggtg gccttacaga
     2821 cggtaaatcc gcagcggcgt acggtggttt ttctttgcgc tccgccgggg acgggtaaat
     2881 ctaccctaac caccttctgg gaatatctcg cgcaacaaga cccggagctg cccgctattc
     2941 aggcgctccc gatggatggt tttcaccatt acaatagctg gctggacgcg catcaattgc
     3001 gccccttcaa aggcgcacca gagacatttg acgttgcgaa actggcggaa aatctgcgcc
     3061 aggtggtgga aggggattgt acgtggccgc agtacgatcg acaaaagcat gatcctgttg
     3121 aagatgcgtt gcacgttacc gcaccactcg tcatcgtcga aggaaactgg ttgttgctgg
     3181 acgacgagaa gtggtgccag cttgcgcaat tttgtgattt ctcgatcttt atcaacgcgc
     3241 cagctgctgc gttgcgggag cgtctggtgg ggcgcaagct ggcgggaggg gtatcgctgg
     3301 cagatgcaga agccttttat gaccgtaccg acgggccgaa tgttcgccgg gggctggaag
     3361 agagtctgcc tgcaaatttg accttaatga tgaccgctac gggtgaatat cgtctggtgg
     3421 attaacgttc ctgattacac catcttccag ccgcaccaca cgagagaaat gacgccgggt
     3481 aaatgcggcg tcatgactga ttgctaccac gctcgtgcct cgctggcggc acttctccag
     3541 ccagctttca aacacgctca accaattttc gtcaaaatcc cgactcggct cgtccagcag
     3601 taatagtggc ggagagagtg cttccaggca ggcgacggcg accatccgtc gctgcgcatt
     3661 atgcagatcc aacgggtgcg agttggcagt gtctgctaac tgacaacatt gcagggcagc
     3721 atgggtacgt tgagtgattt catccgccgg gcatttttgc agtttcaaac caaatgcgat
     3781 ctcgtcagcg acggtgctgt gaaaaagttg gttttccgct tcctgaaaca gtacgccaac
     3841 ctttgccgcg cgctggcggt ttttcaggtt tttcatcgcc tgttgttgca gcataaccgt
     3901 accggctgtg ggcgtaagaa gacctgccat tactcgtaac agcgtcgatt tccctgcgcc
     3961 attgtcaccg gtcagcgcca accattcacc ttgtttgagt tgcagagaaa tgtcgcaaag
     4021 acaatccgtt gccgcgccag gccagcgata agatatctta tttaacgtta acatagtggc
     4081 tggagcgctc cgttttgcaa ttgccacgct tgctggcagt agttcaggaa aggtgtgcga
     4141 tgacgttcaa aaagaataat cagcgaacca cgttcaaacg cccagtgttg taagcgctgc
     4201 aacagcattt cgctggcctg cggtgtcagg cggctaaagg cttcatcaag aattaagagc
     4261 tttggctgca tcgcgatggc gcaggcgatg atcacgcgct gtgtctctcc accggagagt
     4321 gttgccgggt gacgatggcg caacggctgg cactcggtca gagtcagggc ggcatcaatc
     4381 cgcgccataa tctctgcttc agcaagacac agattttctg gcccaaaggc aacttcttct
     4441 tccacgctaa aggtacagcc ggagagctgc aaatagggcg attgctggac gagctggatg
     4501 gtggcggatt gttcattaag cgggagatgc ccaatgggcg taccgaggag cgtccccgtg
     4561 cctgttatct ctcttggcag aaagtccgga taccagcccg ccatcagctg tgccagcgta
     4621 cttttgccac tgccgttgtc accaaagatg gcgaccatcc ccggtttgac atagtgaaag
     4681 tcataacaga acggaggatg cgttgagtgt gtcgggcaat aacggaactg ttctaacgta
     4741 accatatcca cgctccgaat tctgtcagca tcagcaagat catggtgtaa cgcgctacgc
     4801 gttgtagggt gctatcggct ggtgcccaca acgttgttcg tcggttgtgg atccgaaatg
     4861 cccgcatatc cagcgccgca ccacggacag ccagatcgtt aagcgcattg tgtgttagcg
     4921 gaataattaa cgcgggcata gcacgtaaac gctgatacca gccctcatcc agcggcacgc
     4981 cgcgggcacg ctgcgcttca tgaataatcg ctaattgccg ttttaactgc tcaacgacca
     5041 ataacggccc ggcaaacaga taagcgacgc ctggcggcag gcgagaagca aacagagcgc
     5101 gaataaaaca ttgcacgggg acgtactgca ttcacaactg tgaggttgaa acaatcgcca
     5161 gaatcagcaa ccagagcgta atagcatgcg accaccgttc tggcgagcgt ggcgtgccgc
     5221 ttagccattc tggtagccag ccaccgtgta ccagccacag cccggctccc agcgagaaca
     5281 tcagccagac gacatatttt gcccgccgcc gcgtggcctt tagcgcaata aggcagaaaa
     5341 aggtcgccgc gctatagatc ggcaaaatgg tctgcgcggg caggataagc gtggtgcagg
     5401 ccgctaatgc ccacagtgtt aatgaggtaa gtggatgcat ttagcgcacg gcagccattg
     5461 ccggaaaatg gcgcgtggtg cgaatcggca gctggcgcag taaaagccag acaatcaccg
     5521 ctgtaagaat tttatcgacc aggttcgcgc ctatcaccgt aatcgccacc gactccacca
     5581 ggttttgccc catcgagtgc atccaggcaa cgaacaaatc ggctccgcta ccggtgacac
     5641 cgccgaacag cgcagtacgc agcggtacag cgacgaccgt taccgcaagc gtaataatca
     5701 cgccgctgac aacgactttt ggcagggtgc gaaaccaacc cgctcgcgcc agccaaccgg
     5761 agaccaggcc gatgaccatc gcgacgggtg caaatgcggc ggcgatcggg tcggtcagca
     5821 atccccagag taaattggtc agtaagccgg tcagcattcc gatcaccgga ccaagcagta
     5881 ccgcgctaat aagtgtgccg atggagtcga gaaatatagg taatttgacc atgctggcca
     5941 gttgcccgcc gatcatattg atggcgatag atataacgat caacaccagt gcctgactgg
     6001 aaaagtggct acgcgccata agaaaacctc tttgaaagta gttatttaac gggggaaata
     6061 tgcaactcgt cgtaaccaga aagcgcgcaa ggttggcggc taaacgccag ttcctcaagg
     6121 ctaccgatca ccagttcaag cgtggtccgc acggtacagc cgggcatcat atgaccgcca
     6181 agcatcgtgc cgtgtggatc ggaaacgcag agatgaagat gctcaccact ctgttccagc
     6241 gtgccgttta gtgagatcac ttcgaatgta ccgttcagga gcgtggtgcc ttcttgcccg
     6301 gcatagcgca aggcgacgtc agtcaggcta ccggtacagc ctgcaatcta ggcggcatga
     6361 agttgatgtt gctgtgcaaa agcgtgtagc tgggaaaaaa cttcctgccc ggggaggagg
     6421 cgcagtgcgt aaaagcgtgc ggaggaagaa tatgagtagg gcaatgtcat cattggagta
     6481 ctccgaaact cggacgtatt gcggataata tcgacgctgc atcagtcata ttctgatata
     6541 agtcagggtt tgtgacggca caacgaaaag attttgctac gcttattcgt gattctgcaa
     6601 agtggaataa attccacagg ggcggggctg aaaacgtgac gtaagtcaga taattgcttt
     6661 ctttgactgg acaatcattc cttttattcc acgtttcgct tatcctagct gaagcgtttc
     6721 agtcgattaa atgttcgaca attaaccaat cagtcgcagt ttgcgacagg taaggtttcc
     6781 ccggacgatt tgctggatta ctctgtctgg caaatttgct cagggaaaac cttgcaggag
     6841 atctatgacc gtacgcgtag cgataaatgg cttcggtcgc atcgggcgta atgtggttcg
     6901 tgctttgtat gaatccggac gccgggcgga aattaccgtg gtggcaatca acgaactggc
     6961 ggatgctgcg ggcatggcgc atttgttgaa atatgacacc agccatggcc gttttgcatg
     7021 ggaagtacga caggaacgcg atcaactttt tgttggtgat gacgccatcc gcgtattgca
     7081 tgaacgttca ctgcaatcgc tcccctggcg tgaacttggc gttgatgtag tcctcgactg
     7141 caccggcgta tatggctccc gcgagcatgg cgaagcgcat attgccgccg gggccaaaaa
     7201 agtgctcttt tcacatcctg gcagtaacga tctcgacgcg accgttgttt acggcgtcaa
     7261 tcaggatcaa cttcgtgcgg aacaccgcat cgtttctaac gcttcctgta ccacgaattg
     7321 cataattccc gtcatcaaat tgttagatga tgcgtacggt attgagtccg gcactgtgac
     7381 cacaattcac tccgccatgc acgatcaaca ggttattgat gcataccatc ctgacctgcg
     7441 tcgcacccgg gcagccagcc agtcgatcat tccggtcgat actaaactgg ccgctggtat
     7501 cacacgattt tttccgcaat ttaacgatcg ctttgaagcg attgcggtac gtgtgccaac
     7561 cataaatgtg acggcaatcg atttaagcgt gacggtgaag aaacctgtaa aagccaatga
     7621 agtcaacctg ttgctgcaaa aagcagcaca aggtgcattt catggtatag ttgactatac
     7681 ggaattgccg ttggtctctg tagattttaa ccacgatccg cacagtgcca ttgtcgatgg
     7741 cacccaaacc cgggtcagtg gcgcacacct gatcaaaacg ttggtctggt gcgataacga
     7801 atggggcttt gctaaccgaa tgctcgacac gacgttagct atggctactg ttgctttcag
     7861 gtaagacgca agcagcgtct gcaaaacttt tagaatcaac gagaggattc accatgtctg
     7921 taattaagat gaccgatctg gatcttgctg ggaaacgtgt atttatccgt gcggatctga
     7981 acgtaccagt aaaagacggg aaagtaacca gcgacgcgcg tatccgtgct tctctgccga
     8041 ccatcgaact ggccctgaaa caaggcgcaa aagtgatggt aacttcccac ctgggtcgtc
     8101 ctaccgaagg cgagtacaac gaagaattct ctctgctgcc ggttgttaac tacctgaaag
     8161 acaaactgtc taacccggtt cgtctggtta aagattacct cgacggcgtt gacgttgctg
     8221 aaggtgaact ggttgttctg gaaaacgttc gcttcaacaa aggcgagaag aaagacgacg
     8281 aaaccctgtc caaaaaatac gctgcactgt gtgacgtgtt cgtaatggac gcattcggta
     8341 ctgctcaccg cgcgcaggct tctactcacg gtatcggtaa attcgctgac gttgcgtgcg
     8401 caggcccgct gctggcagct gaactggacg cgctgggtaa agcactgaaa gaacctgctc
     8461 gcccgatggt ggctatcgtt ggtggttcta aagtatctac caaactgacc gttctggact
     8521 ccctgtctaa aatcgctgac cagctgattg ttggtggtgg tatcgctaac acctttatcg
     8581 cggcacaagg ccacgatgtg ggtaaatccc tgtacgaagc tgacctggtt gacgaagcta
     8641 aacgtctgct gaccacctgc aacatcccgg ttccgtctga tgttcgcgta gcaaccgagt
     8701 tctctgaaac tgcaccggct accctgaaat ctgttaacga tgtgaaagct gacgagcaga
     8761 tcctggatat cggtgatgct tccgctcagg aactggctga aatcctgaag aatgcgaaaa
     8821 ccattctgtg gaacggtccg gttggcgtgt tcgaattccc gaacttccgc aaaggtactg
     8881 aaatcgtggc taacgctatc gcagacagcg aagcgttctc catcgctggc ggcggcgaca
     8941 ctctggcagc aatcgacctg ttcggcattg ctgacaaaat ctcctacatc tccactggcg
     9001 gcggcgcatt cctcgaattc gtggaaggta aagtactgcc tgcagtagcg atgctcgaag
     9061 agcgcgctaa gaagtaaaaa atcacagggc agggaaacct gcacttgttt cagcgcgctt
     9121 ttagagcacg cattttttca tactctaaat aattcgagtt gcaggaaggc gacaagagag
     9181 tgaatcccca ggagcttaca taagtaagtg actggggtga gcgaacgcag acgcagcaca
     9241 tgcaacttga agtattatga gtataaggcc cgacgataca ggacaagaga catgtctaag
     9301 atttttgatt tcgtaaaacc tggcgtaatc actggtgatg acgtacagaa agttttccag
     9361 gtagcaaaag aaaacaactt cgcactgcca gcagtaaact gcgtcggtac tgactccatc
     9421 aacgccgtac tggaaactgc tgctaaagtt aaagcgccgg ttatcgttca gttctccaac
     9481 ggtggtgctt cctttatcgc tggtaaaggc gtgaaatctg acgttccgca gggtgctgct
     9541 atcctgggcg cgatctctgg tgcgcatcac gttcaccaga tggctgaaca ttatggtgtt
     9601 ccggttatcc tgcacactga ccactgcgcg aagaaactgc tgccgtggat cgacggtctg
     9661 ctggacgcgg gtgaaaaaca cttcgcagct accggtaagc cgctgttctc ttctcacatg
     9721 atcgacctgt ctgaagaatc tctgcaagag aacatcgaaa tctgctctaa atacctggag
     9781 cgtatgtcca aaatcggcat gactctggaa atcgaactgg gttgcaccgg tggtgaagaa
     9841 gacggcgtgg acaacagcca catggacgct tctgcactgt acacccagcc ggaagacgtt
     9901 gattacgcat acaccgagct gagcaaaatc agcccgcgtt tcaccatcgc tgcgtccttc
     9961 ggtaacgtac acggtgttta caagccgggt aacgtggttc tgactccgac catcctgcgt
    10021 gattctcagg aatatgtttc taagaaacac aacctgccgc acaacagcct gaacttcgta
    10081 ttccacggtg gttccggttc tactgctcag gaaatcaaag actccgtaag ctacggcgta
    10141 gtgaaaatga acatcgatac cgatacccaa tgggcaacct gggaaggcgt tctgaactac
    10201 tacaaagcga acgaagctta tctgcagggt cagctgggta acccgaaagg cgaagatcag
    10261 ccgaacaaga aatactacga tccgcgcgta tggctgcgtg ccggtcagac ttcgatgatc
    10321 gctcgtctgg aaaaagcatt ccaggaactg aacgcgatcg acgttctgta agtcattcct
    10381 ttctgcttat ctcaaggccc gctctgcggg tctttttttc gccaaaagca aggtgattca
    10441 gatgagaaat ctgtgatcta tttggcaaaa ttatgcttta ttgtttaccc ttgtcagact
    10501 gcccgtcata aggcggcgga gtgtatttct ccattttgag tcagttgaaa aggaatattg
    10561 aatggaagat ttgaatgttg tcgatagcat aaacggcgcg ggaagctggc tggtagctaa
    10621 ccaggcgctg ctgctaagtt atgcagtaaa catcgtggcg gcactcgcga tcatcatcgt
    10681 tggtttgatt atcgcgcgga tgatttccaa cgcggtgaat cgcctgatga tctcccgtaa
    10741 aatcgatgcc actgttgctg attttctttc tgcattagtc cgttacggta ttatcgcctt
    10801 tacgctaatc gctgccctgg gacgcgtggg tgtacaaaca gcgtcagtca ttgctgtact
    10861 cggtgccgca ggcttagctg ttggtctggc tttgcagggg tcactttcta acctggccgc
    10921 tggcgtgtta cttgtcatgt tccgcccgtt ccgtgccgga gaatatgttg acctcggcgg
    10981 cgtagccggt actgtgctga gtgtgcagat tttctccacc accatgcgta ctgcagacgg
    11041 taaaattatc gttattccga acggtaaaat tattgccgga aatattatta acttctcccg
    11101 cgagccagtt cgccgtaacg aatttattat tggcgtggcg tatgattccg atatcgatca
    11161 ggttaagcag atcctgacca atattatcca gtctgaagat cgcattttga aagatcgcga
    11221 aatgactgtg cgcctgaacg aacttggtgc atcgtcgatt aatttcgtgg tccgcgtctg
    11281 gagcaacagc ggcgatctgc aaaacgtgta ctgggatgtg ctggagcgta ttaaacgtga
    11341 atttgatgcc gccggtatca gcttcccgta cccgcaaatg gatgtgaact ttaagcgggt
    11401 gaaagaagac aaagctgcgt aatcaacgct gaggccagat aatactctct ggcctctctc
    11461 ttattagtta ttctgattgc caattaatat tatcaatttc cgctaataac aatcccgcga
    11521 tatagtctct gcatcagata cttaattcgg aatatccaac gtgttttctt attactttca
    11581 aggtcttgct cttggggcag ctatgattct gccgctcggc ccacaaaatg cttttgtgat
    11641 gaatcagggc attcgtcgtc agtaccacat tatgattgcc ttactttgcg ctatcagcga
    11701 tttggtcctg atttgcgcgg ggatttttgg tggtagcgcg ttattgatgc agtcgccgtg
    11761 gttgctagcg ctggtcacct ggggcggcgt agtcttcttg ctctggtatg gttttggcgc
    11821 ttttaaaaca gcgatgagta gtaatattga gttagctagt gccgaagtcc tgaagcaagg
    11881 cagatggaaa attatcgcca ccatgttggc agtgacctgg ctgaatccgc atgtttacct
    11941 ggatactttt gttgtactgg gcagccttgg cgggcagctt gacgtcgaac caaaacgttg
    12001 gtttgcgctc gggacaatta gcgcctcttt cctgtggttc tttggtctgg ctattctcgc
    12061 agcctggctg gcaccgcgcc tgcgcacggc aaaatcacag cgcattatca atctggtagt
    12121 gggatgtgtc atgtggttta tagccttgca gctggcgaga gacggtattg ctcatgcaca
    12181 agccttgttc agttaggcgc tatctgatgg aaaaataaaa cagaggcgct aagcttgcct
    12241 ccagaggtcc tgaattttgg gcaagtcaat taacacggag agactaacgt gaagttcaaa
    12301 gttatcgccc tggcggcatt aatgggtatt agcgggatgg cagcgcaggc taacgaattg
    12361 ccggatggac cgcatattgt cacctccggt acggcaagtg tggatgcggt gccagacatt
    12421 gccactcttg cgattgaagt taacgtggcc gcgaaggatg ccgctacagc caagaaacag
    12481 gcagatgagc gcgtcgcaca atacatttcc ttccttgaac tcaatcagat cgcgaaaaaa
    12541 gatatcagct cagcgaattt acgcacccag ccagattatg actatcagga tggtaaaagt
    12601 atcctgaaag gctaccgagc tgtgagaacg gtggaagtca cgctccgtca gttagacaaa
    12661 ctgaattcct tgctggatgg cgcgctgaag gcgggtctta acgaaattcg ttctgtgtcg
    12721 ctgggcgtgg cgcagccgga tgcctataaa gacaaagcgc gtaaggcagc gattgataac
    12781 gcgattcatc aggcgcagga actggcgaac ggctttcatc gtaaactggg gccggtatat
    12841 agcgtgcgct accatgtttc caactatcag cccagcccaa tggtgcggat gatgaaagcc
    12901 gatgccgcgc cggtgtctgc ccaggaaact tacgagcagg ccgctattca gtttgatgat
    12961 caggtcgatg tggtcttcca gttagaacct gtggatcaac aacccgctaa aacacctgca
    13021 gcacaataat acttgtagcc cgacgtattc gtcgggctaa tgcctttacc cgatattgcc
    13081 tcctttttct gtaattttct gtaaaaccat gacgtaatca cctatctttg ctcgcggttt
    13141 catcaaggat aggctgactg aaacggttaa gcggacaagg atgcggggag gggggatgga
    13201 catttttatt tcgaaaaaga tgcgcaattt cattttatta gcgcaaacca ataatattgc
    13261 ccgggcggca gaaaaaatcc atatgacggc ttcgccattt ggtaaaagca ttgccgcgct
    13321 ggaagaacaa attggctata cgctatttac ccgcaaagat aataatatca gcctcaataa
    13381 agccggtcag gaactgtatc aaaaactgtt cccggtttat caacgacttt ctgcgattga
    13441 taatgaaatc cataactcag ggcgtcgttc acgagaaatt gtgattggca tagataatac
    13501 ttatccgaca attattttcg atcagttgat tagtctcgga gataagtatg aaggcgtgac
    13561 cgcccagcca gttgagttca gcgaaaacgg cgttatcgat aatctttttg accgtcagtt
    13621 agattttatt atctccccac agcatgtatc tgcacgagtt caggaactgg aaaacctgac
    13681 tatcagtgaa ttaccaccgc tgcgattggg ctttctcgtt tctcgccgtt atgaagagcg
    13741 tcaagagcaa gagctgcttc aggagctgcc ctggctacaa atgcgctttc aaaaccgggc
    13801 taacttcgaa gcaatgattg atgcgaatat gcgaccttgt gggatcaatc ccaccattat
    13861 ttatcgtccc tacagtttta tggccaaaat cagcgccgtg gagcgcggac attttctgac
    13921 ggtgatcccg cattttgcct ggcgactggt gaatccggca acgctgaaat attttgatgc
    13981 gccgcacagg ccgatgtata tgcaggaata tctttattca atcagaaatc atcgttatac
    14041 cgccacgatc ttcagcatat tgctgaagat cgtgacggga caaaccatta acccagcatc
    14101 gagccggttg caattaaatt acggtgtaag tcgaagacgt ggctaagatc gtggtgaata
    14161 tgtccgccgg gcgcattttc cagatagcga tgcagataat cccgatacat aggatgtgca
    14221 caattatcaa tgatagtgcg ggcgcgttga agcggggaaa gaccgcgcag atcggcgatc
    14281 ccttgttcgg tgatgatcac tttgacgctg tgttcgctgt gatcaacatg gctgcacatt
    14341 ggcacgacgg ttgagatctt gccttcttta gcaatcgacg gagccataaa gatcgacaga
    14401 tacgcgttgc gttcaaaatc accgctgccg ccgatgccgt tcatcagatc gaccccggct
    14461 acgtgcgttg aatttgcatg cccgtaaata tcaaactcca ggccgacgtt cagagcgatg
    14521 acgcccagac gacggatgat ttccgggtta ttggaaatct cctgcggacg caacacaatg
    14581 cggctggcaa agtaatccat attgtcgtaa atcttgcgca gggaatcggc cgagattgtc
    14641 aggctggagg cgctggcccc gctgattttg ccggtttcca gtaaatgcac caccgattcc
    14701 tgtagcactt ccgaatacat catgaacgga ggaatttccg ggttttcccc cagacgtgcc
    14761 attaccgcat tattgatatt gcccacgcca ctttgcagcg gcagaaattc cggcggaata
    14821 cgcccatgcg ccatttcctg caataagaac gtgaccacgt tatcggcaat ctgctggcac
    14881 atgggatttt gcttatccag catattaccg gcgtcgggca agttggtttc cacgacggcg
    14941 acaatctttt tcggatcgat ttgcacatag cgggtaccga cgcgatccat tgcatggaag
    15001 atcgacacgc tattgcgccg cggtggcgcg ccaggaatca caatatctgc cagttctgca
    15061 acgcgcggat cgtgatagtg attaagttca atgatcactt tcttcgctcg cagcagctag
    15121 gtcggcgcat taccgatccc gctggttaac cagactcgac catccggtgc cagtgccgat
    15181 gcttcaatga cggcaacatc aatgtcgccg aagaaaccgt aattgaccat ttgcgccact
    15241 tcgctcaaat gcaggtcaac gaaactcacc gcgccctgat tgatcttttt acgtaaactg
    15301 gacgatgttt gatatggcgc acgccaggaa acagcatcgg cgtcagaaag tacatcgtca
    15361 gcggcggcgc tgattgacgc ccccgtcagc aggcgaattt gatacggctt tttggcctca
    15421 tgctgttcgt tagctctgcg ggcaatcgcg gcgggtaggg ctttcggcga acccgctggg
    15481 gtaaagccgc taaatgccac catgtcgtta tgctggataa tttctgccgc ttcatcggcg
    15541 gtcatccttg tccactgagt ttccattttt actccttcgt tcgcagggat taatgaccaa
    15601 cgaaattagg tttacgtttt tcgaggaaag cgttcatccc ttcctggtaa tcttcgctgt
    15661 cataaaccgc gcggcgcatc ccctgaatac gttcaaattc atcggagttc atggtgtgtg
    15721 cttcgcccag tacacgcagc tcttctttga taacggcaat ggctaacggc gctttctcag
    15781 agatgtggtg cgccatttgt aaggtgaaat cttccagttc ttccacttcc acaacatggt
    15841 tgaggatgcc gacagccagc gcgcgctggg cggtgattgg cgaagcggta aaaatcagct
    15901 ctttgacaat gtggaagccc gcgtcgcggg tcaggttgtg aatgccgacc aggttatacg
    15961 ggacgccgag gtttacaggc gtcattgaga aggttgaggt actggcggcg atgatcagat
    16021 cggaactcat gatcatttca aatgcgccac cccaaacact accttccacc atcgaaatga
    16081 tcggtttcgg gaatttttgg atcatgcggg tgatttgacg caatggatca tcataggaga
    16141 gcggatcgcg accgccagac ggcagttcgt gaatatcgtg acctgcggag aagactttgg
    16201 atccacttgg cgcccgcaaa atgatacagc gaatttccgg ccggttgaga tcgcttaacg
    16261 cctgcataag atcatcaata aagactttac ttaaggcatt aagttttcgt ccatagttaa
    16321 actcaatgac cgccactttg ttgatagtgg caacgttcac atactgataa gacataaaaa
    16381 ttcctttaat caaaatattg cgtctggata aattcactga gctgccgcag gccggtgcgc
    16441 ggtgagagcg tattgttttt gaccgctaaa agcgtctggc ggtaatagcg atcgaaatct
    16501 tcattcgcga acaggtgatt cagtacttct tcttcggtct gcttacgcag ccattccacc
    16561 gattgttgtt gccgcacttg ttgtaaacga ccactggcag ttagcgcggt tttgaagtcg
    16621 atgatggcgt gccagatctc atcgattcca cgtttttcca gtgcgctaca agtcagaccc
    16681 cgtggctgcc attcgtcgta tttacgtcgc agaatatgca gggcactctc gtacatatgc
    16741 cgggcaatgg cgacattggt atggttatcg ccatcgtctt tgttgataac gagcagatca
    16801 gccacttcca tcagcccttt tttaatgccc tgcagatcat cgccgccacc ggcaatttgc
    16861 aacgagataa aacagtccac catgcgggcg acttccgttt ccgactgcgc gatgccaacc
    16921 gtttcgacaa tcactacgtc ataacccgcc gcttcgcata acagcattaa ttcccgtgct
    16981 cgctgactgg caccgcccag atgaccggag gagggtaccg ggcgaataaa cgccgcctcg
    17041 gcacgcgcca ggtcattcat gcgggtttta tccccgagaa tgctaccgcc agtgaccggg
    17101 ctgctgggat cgaccgcaat aacagcaacc tttaatccct cacgaatcaa caacatgcca
    17161 aaggcttcaa gaaaggtact tttccccgcg ccgggtgtgc cggtaacgcc cagtcgcagg
    17221 gcgttaccac agaacggcat aatagcgtca agcagctgtg tacttagcgc ctgatgacgt
    17281 gggtgacggc tttccaccag cgtcatggct tgggcgagcg tggcacgctc accctgacgt
    17341 aagcggcgaa tactttctgc cagcgtggct tcattaatca tgatgctggc ttatcagatt
    17401 cagtacgtcg cgcacactgt cgagcatagg tgtacctgga ccataaatcg ccgccacgcc
    17461 gcgctcttgc aggaaggcgt aatcctgcgg cggaatgacg ccacccgcga ccacgcagat
    17521 atcttcgcgt ccccattttt tcagcgcttc gaccagttcc gggatcagcg ttttatgacc
    17581 ggcagccagt gaggatgcgc ccactacgtg aacgtcgttt tctacggcta ggcgggcgat
    17641 ctcttcaggt gtagagaaca tcgggcttaa atctacgtcg aaaccgagat cggaataggc
    17701 gctggcgatc actttcgcgc cgcgatcgtg tccatcctgg cccatcttag cgatcagaat
    17761 gcgcgggcga cgaccattgt cggcaaggaa ctgctccgtt tgcgcaacaa tggcatcgaa
    17821 ctcggaggcc gatttctcag actgatgata gctttgcgca atcacgccgg taacacactg
    17881 gcttggcacc agataacggt cgaaagcgac ttccagcgca tcggaaattt cacccagggt
    17941 ggcgcgaacg cgagcggcat taacagcggc agccagcagg ttttcgttat gctgtgcggc
    18001 gtgagtcagg gcgttcaacg cggcggttac ggcggcatca tcacgggtgg cgcgaatgcg
    18061 ttccagcgaa gcaatttgct cgttacgcac catcacgttg tcgatctcaa gtacatcggt
    18121 ttcgtcttcg tgatccagtt tgtacttgtt gacaccaacg atgacacgct tgccctggtc
    18181 gatcagcgac tgttcgcgcg ctgaggcctc ttcgatcatt cgttttggca gacctgcttc
    18241 gatcgctttc gccatgccac cggcttcgtc gatctgttgg ataatagctc tggcttgttt
    18301 gacgatttga tcggtcagcg actcaatgta ataggatccg gccagtggat cgacggtgtg
    18361 gcagagttct gattcttcct ggatgatgat ctgggtgttg cgggcaatgc gtgctgagaa
    18421 atcggtaggc aaaccaagcg cttcgtcaaa ggcgttggta tgcagtgact gagtaccgcc
    18481 cagcgtcgca gccagtgctt caatggtggt gcggataacg ttgttatacg gatcctgttc
    18541 agtcaggctc cagcctgagg tctggcagtg ggtacgcagc gccagtgatt tcggatcctg
    18601 tgcgccaaat ccactgaccg cttcgctcca taaataacgt gccgcacgca acatggcgac
    18661 gttcataaac agatccatgc cgataccgaa gaataacgac aggcgaggag cgaagtcatc
    18721 aattttcagt ccggcagaga ttgctgcttt gatgtactca atcccatcgg cgagcgtaaa
    18781 tgctacctgc tgcacgcagt tggcacccgc ttcccccatg tggtaaccgc tgatactgat
    18841 ggtattaaat cgcggcatgt tgccggaaca ccaggcgatg atgtcggcga taatgcgcat
    18901 tgacggtttt ggtgggtaaa tataggtgtt gcggcagagg tactctttga gaatatcgtt
    18961 ttgaatggtg ccggtcagtt tatcaggtgt aacaccttgc tcttctgcgg cgacgatata
    19021 aaacgccagt actggtagca ctgcgccatt catggtcatc gaaaccgaca ttttatccag
    19081 cgggatctgg tcgaacagga ctttcatatc ttccacggtg tcgatagcga cgcccgcttt
    19141 gccgacgtca cccgccacgc gtgggttatc ggagtcgtag ccacggtggg tggcaaggtc
    19201 aaacgcaacg gaaagacctt tttgcccggc ggccaggtta cggcgataaa aagcgttgga
    19261 ctcttttgct gttgaaaaac cagcatactg acggatggtc cacggttggg cggtatacat
    19321 agtggcacgc gggccacgaa cgtagggcgg caaaccagga agggtacctg tcacctccag
    19381 attatcgaga tcggcttcgg tatacagcgg cttgatggcg atcccttccg cggtttgctg
    19441 aaccagcgag tcgacagttt tctcccgacg gctcaattcc ttgttggcaa gctgttgcca
    19501 ctcctgctcg ttagacatta tttgctccgt aaaacgcaat caaaatgagg gtatttgcgc
    19561 ttacggtgag ctttttacgt gggtttgtcg ccagcaaaaa aaatgcattc agacgtcgtt
    19621 ttttgccgcc tgagatttgt ctattttgtg atgtgattca aggaatacaa aaagaagtga
    19681 ggacaacgcc tgatatgtgc tatcaggcat tatttgatgg attaatcctg acgaaggact
    19741 ttgtgaccat aatcgagtaa cgcatcagtg actttacgca tcatgcggct ttcaggagca
    19801 aagcggtgcc agtagagcat ccgtcgttga aatagcccag gcgttaagtc aatcagttca
    19861 ccgctggcca gctctttctc gatttgcagg tgcgggatca tacagcaggt ggtgccctgg
    19921 cgagcaagtt gtacgaacgc ttctgaagaa ttaacgatat ggcagggtac gctgccagga
    19981 ggaagatcga agttttgctg caaaaaggcc tggtgcatat cgtcaagatg gtcaaacgcg
    20041 accactggcg ctttcaataa tgccgaacgc gttacgccgt taggaaaata tttttcggca
    20101 aacggttttg agctgacgaa cagatagtcg agcgcaccga gtttatcgac aagacaactc
    20161 ggcagcgcct gatgttgaat actcaccgcg ccgaccactt cgccgcggcg cagacgttcc
    20221 tgagtacggg tttcatcttc tacctgcaag ttgaggcgga taggcgaatc agccaacaca
    20281 ggagccagtg ccggaagcaa ccacgtcgcc agactgtcgg cgttgaccgc cagtgaaagc
    20341 agcagcggag tcgaaccggt ttgttcatcg cccagccact cttcttccag caactccacc
    20401 tggcgcagca gtgccagcag tttttgccct tgttccgtcg ggcgcggcgg tacggtacgc
    20461 accaacagcg gctgcccgaa catattttcc agttgcttaa tgcgctgtga gacggctgat
    20521 tgtgtaatgc acagcttttg tgccgcgcgc tcaaatcctc gttcacgtat caccgcatcc
    20581 agtgcctgta atgttctgta gtccgggcgt ttcattgctg ttgtctgctc ctggtaaatg
    20641 ctttttcctg cactatgaca caattttgtg tcaggttgca tataccgaag ccgtcggcgg
    20701 gtaacatttg tatcaatggt taaacgcgca gcttaaaaga tcagggattt gcgctattgc
    20761 atttcgctta gcgacttcgc gccctggtta tgttgaatgg cgtggcgtta ttgcctctgt
    20821 tcgcaccgtg gagcggcata cccagaccgt tgtatgcttt atttgcgggg cgaaagggga
    20881 tgcccgccat tgcgcgatat tttatggatg agttaaccac ccggcttgcc aacggggtct
    20941 gaatcgcttt ttttgtatat aatgcgtgtg aaatttcata ccacaggcga aacgatcatg
    21001 acgcaggatg aattgaaaaa agcagtagga tgggcggcac ttcagtatgt tcagcccggt
    21061 accattgttg gtgtaggtac aggttccacc gccgcacact ttattgacgc gctcggcacg
    21121 atgaaaggcc agattgaagg ggccgtttcc agttcagatg cttccactga aaaactgaaa
    21181 agcctcggca ttcacgtttt tgatctcaac gaagtcgaca gccttggcat ctacgttgat
    21241 ggcgcagatg aaatcaacgg ccacatgcaa atgatcaaag gcggcggcgc ggcgctgacc
    21301 cgtgaaaaaa tcattgcttc ggttgcggaa aaatttatct gtattgccga cgcctccaag
    21361 caggttgata ttctgggtaa attcccgctg ccagtagaag ttatcccgat ggcacgtagt
    21421 gcagtggcgc gccagctggt gaaactgggc ggtcgtccgg aataccgtca gggcgtggtg
    21481 accgataatg gcaacgtgat cctcgacgtc cacggcatgg aaatccttga cccgatagcg
    21541 atggaaaacg ccataaatgc gattcctggc gtggtgactg ttggcttgtt tgctaaccgt
    21601 ggcgcggacg ttgcgctgat tggcacacct gacggtgtca aaaccattgt gaaatgatct
    21661 gacgggggaa cctcccccgt taaaaaaatt ctcttcatta aatttggtga catgtgtcac
    21721 gcttttacca ggcaattgtc gattgctcta aataaatcct ctaaaccagc atattcatcc
    21781 aagaattacc tttgcgtgat atttcctcaa catcgcgacg caaacgttca tattgccgca
    21841 atattatttt ttgatatgtt gaaaggcgga tgcaaatccg cacacaacat ttcaaaagac
    21901 aggattgggt aaatggcaaa ggtatcgctg gagaaagaca agattaagtt tctgctggta
    21961 gaaggcgtgc accaaaaggc gctggaaagc cttcgtgcag ctggttacac caacatcgaa
    22021 tttcacaaag gcgcgctgga tgatgaacaa ttaaaagaat ccatccgcga tgcccacttc
    22081 atcggcctgc gatcccgtac ccatctgact gaagacgtga tcaacgccgc agaaaaactg
    22141 gtcgctattg gctgtttctg tatcggaaca aaccaggttg atctggatgc ggcggcaaag
    22201 cgcgggatcc cggtatttaa cgcaccgttc tcaaatacgc gctctgttgc ggagctggtg
    22261 attggcgaac tgctgctgct attgcgcggc gtgccggaag ccaatgctaa agcgcaccgt
    22321 ggcgtgtgga acaaactggc ggcgggttct tttgaagcgc gcggcaaaaa gctgggtatc
    22381 atcggctacg gtcatattgg tacgcaattg ggcattctgg ctgaatcgct gggaatgtat
    22441 gtttactttt atgatattga aaacaaactg ccgctgggca acgccactca ggtacagcat
    22501 ctttctgacc tgctgaatat gagcgatgtg gtgagtctgc atgtaccaga gaatccgtcc
    22561 accaaaaata tgatgggcgc gaaagagatt tcgctaatga agcccggctc gctgctgatt
    22621 aatgcctcgc gcggtactgt ggtggatatt ccggcgctgt gtgatgcgct ggcgagcaaa
    22681 catctggcgg gggcggcaat cgacgtattc ccgacggaac cggcgaccaa tagcgatcca
    22741 tttacctctc cactgtgtga attcgacaac gtccttctga cgccacacat tggcggttcg
    22801 actcaggaag cgcaggagaa tatcggcttg gaagttgcgg gtaaattgat caagtattct
    22861 gacaatggct caacgctctc tgcggtgaac ttcccggaag tctcgctgcc actgcatggt
    22921 ggtcgtcgtc tgatgcacat ccacgaaaac cgtccgggcg tgctaactgc gctgaacaaa
    22981 atcttcgccg agcagggcgt caacatcgcc gcgcaatatc tacaaacttc cgcccagatg
    23041 ggttatgtag ttattgatat tgaagctgac gaagacgttg ccgaaaaagc gctgcaggca
    23101 atgaaagcta ttccgggtac cattcgcgcc cgtctgctgt actaattccc cttctctgaa
    23161 aattaacggg caggtcactg acttgcccgt ttttttatcc cttctccaca ccgttgccgc
    23221 gttgcgcatt ctactttgcg agtctgctcc agaaaataaa tcaaagtcag atcaatgcgt
    23281 tgtgtttatt taatggggat ttacgatggc gggacagcat ggggctgtaa cggtaaagcc
    23341 ctcaccgaag cgagggctta aaggagaagg gttatgatgc gacttgtcat catactgatt
    23401 gtactgttac tgataagttt cggagcttat taacagtcag tctcaaggga ggagtcatcc
    23461 tcccttaccc ttactcacta aattaggtca aagaatcaac gatgtcaatc agggcgatgc
    23521 ggggtgcatc gccctgaacc gaactttttg atagccactt catcgcccac gtttactgcc
    23581 gtaatagatc ctttttccca aaaattatct ctgttttgca tggggttttt cgagcacgct
    23641 cgaagaaaag atttaaaggt agatcaatgc gttgtggtta tttaaagaga gcttacgata
    23701 gctgggcagc atggggctgt aacggtaaag ccctcaccga agcgagggct tgaaggagaa
    23761 gggttatgat gcgacttgcc atcatactga ttgtactgtt actcataagt ttcccagctt
    23821 attaacagtc agtcacaggg gaggagcaat cctcccttac ccttactcac taaattaggt
    23881 taaagaatca acgatgtcaa tcagggcgat gcaggtgtat cgcccttacc actcccagac
    23941 tttcgacggt gtaaccaccg caggaagagg gatatcccac tcttcaacgg ggagtttttc
    24001 caccaactga caatcatgcg cataacccac cggttgcgtt ttatagtgct gccagttttg
    24061 taaggtccga tcataaaaac caccgcccat tcccagacgc tgaccgtact catcaaaggc
    24121 gaccagcggt gtgatcagca cgtctaatcg ggaaaggggt agcacgtcac gcacatccag
    24181 ttttggctca tggatcttca acctgttcat caccagttcg ctttgcggat ggtaattcag
    24241 gaacagcaaa ttaccggcac taaagggatg caaaactgga agatatacgc gcttaccggc
    24301 gcgccagagt tgttctatta gcggctgggt gtcgagttca ccatcaaaag agaggaatac
    24361 agcgaccgta tgcgccatca ccaccggggg ataagtcatc atccgggtag cggcttgttg
    24421 acccatttcc agctgttgtt ccggcgttaa cgcacgacga cgttgccgaa tcattttgcg
    24481 gatttcttgt cgggataatg tcagtgggag ctctggtagt tgcgtcatgg ctggtgccag
    24541 ataagaaggg aatctccgag atgccgccgc aggctgtaac ccttgaaccc ttggttcaag
    24601 gtgaatgtgt cgtcgcagtt ttaaggcttc tcggacgaac cgagcatgct caccaaccgc
    24661 ggagcgccac attcttgtgg tatgaaatat cggctcaggg gactggcccg cttgcgaaca
    24721 tctcagagaa attttgtctt cacggttact ctaccacagt aaaccgaaaa gtgtcattca
    24781 aagttttggt tagttttttc ggtgatgcga ccttgttcaa gtaacgcttg ttctatggtc
    24841 tgctgcagca tccgaatacg ctgttccata cttgccgcgt agtcacgagt ctttgctttt
    24901 tcttgcgcta actcatagct gatattcaat gcggcaatga agaccaactg ttcagtattt
    24961 gtgactctag tgcgttcttt cagatcttgc aaccgttggt tcagatcgtc cgctgcctga
    25021 ttcaacgcat ccctttggtc aggcgggcag ttcacacgca gtgaacggcc aaaaatttgg
    25081 atatcgacgg gttgtgcaga catgccacct tcctgctgat tgactgcgct gccttcgtct
    25141 ttagacccat ggtctgcgaa ggggcgacac tatagctacc ctgatgagaa gagacaagcc
    25201 cttttctggt ccaccagggc ccaaagtggt agcatatcat gaatattcct ccctttgacg
    25261 acgaatgctt atgtctatac agaacgaaat gcctggttac aacgaaatga accagtatct
    25321 gaaccaacaa gggacgggtc tgacccctgc cgagatgcat ggtttaatca gtgggatgat
    25381 atgtggcggt aacgatgaca gctcatggct gccgctactt cacgacctga cgaacgaagg
    25441 catggctttc ggtcatgagc tggcacaggc actgcgtaaa atgcactctg ccaccagcga
    25501 tgccctgcag gatgacggct tcctttttca gctttatctg cctgatggcg atgatgtcag
    25561 cgttttcgat cgggctgatg cactggcagg ttgggtcaat cacttcctgc ttggtcttgg
    25621 cgttacgcaa ccgaagctgg acaaagtgac cggcgaaacc ggtgaagcca tcgacgatct
    25681 gcgtaacatc gcgcagctgg gctacgacga agacgaagat caggaagagc ttgaaatgtc
    25741 gcttgaagag atcatcgaat acgttcgtgt tgccgcgctg ttatgccacg acacctttac
    25801 tcatccgcaa ccgaccgcgc cagaagtaca aaaaccgact ctacactaaa aacaaaaaac
    25861 gtaaggagag tgttatgagt gagatatccc ggcaagagtt tcagcgtcgc cgtcaggccc
    25921 tggtggagca aatgcaaccc ggcagcgccg cgctgatttt tgctgcacca gaagtaacac
    25981 gtagcgccga cagcgaatac ccctatcgtc agaacagtga cttctggtac ttcaccggct
    26041 ttaacgaacc ggaagcggtg ctggtgctga ttaaaagcga tgacactcat aaccacagcg
    26101 ttctgtttaa ccgcgttcgc gacctgacgg cggagatctg gtttggccgt cgcttaggcc
    26161 aggatgccgc gccagagaaa ctgggcgttg accgcgcact ggcattcagc gaaatcaatc
    26221 agcaacttta tcaactactt aacggcctgg atgtggttta ccatgcccag ggcgaatatg
    26281 catatgctga tgaaatcgtg aacagtgcgc tggaaaaact gcgtaaaggt tcgcggcaaa
    26341 atctcaccgc accggcaacg atgatcgact ggcgtcctgt tgttcatgaa atgcgcctgt
    26401 tcaaatcgcc agaagagatt gccgtactcc gccgcgcggg agaaatcacc gccatggcac
    26461 atacacgggc gatggaaaaa tgccgtccgg gaatgttcga gtaccatctg gaaggcgaaa
    26521 ttcaccacga atttaaccgc cacggtgcgc gctatccttc ctataacacc attgtaggca
    26581 gcggtgaaaa cggctgcatt ctgcactaca ccgaaaacga gagtgaactg cgcgacggtg
    26641 acctggtgtt gattgacgcg ggttgtgaat acaaaggtta cgcgggcgat attacccgca
    26701 ccttcccggt caacggcaaa ttcacccagg cccagcgtga aatctacgac attgtgctgg
    26761 agtctctcga aaccagcctg cgcctgtatc gtccgggaac ttccattctg gaagtcactg
    26821 gtgaagtggt gcgcatcatg gttagcggcc tggtaaaact cggcatcctg aaaggtaatg
    26881 ttgatgaact gatcgctcag aacgcccatc gtcctttctt tatgcatggc cttagccact
    26941 ggttaggact ggatgtccat gacgttggcg tttatggtca ggatcgctcg cgcattctgg
    27001 aaccggggat ggtactgacc gtagagcctg gtctgtatat cgcgccggat gcggatgtgc
    27061 cagaacaata tcgcggtatc ggcattcgta ttgaagacga cattgtgatt accgaaaccg
    27121 gcaacgaaaa cctcaccgcc agcgtggtga aaaagccgga agaaatcgaa gcgttgatgg
    27181 ctgctgcgag aaagcaatga gcgtaatcat cgtcggtggc ggcatggcgg gcgcgacgct
    27241 ggcgctggct atttcccggt taagtcacgg ggcgctgccg gtacatttga ttgaagcgac
    27301 tgcgccagag tcacatgctc atccgggctt tgatggacga gcgatagcgc tggcggcggg
    27361 tacctgtcag caactggcgc gcatcggcgt ctggcaatct ctggctgatt gcgcaaccgc
    27421 catcactacc gtgcatgtca gcgatcgtgg tcatgctgga ttcgtcaccc tcgccgcaga
    27481 agattaccaa ctggcggcgc tggggcaggt tgtcgaattg cataatgtcg ggcaacggct
    27541 gtttgcattg ctgcgaaaag cacctggcgt aatgctgcat tgccccgatc gcgtagctaa
    27601 cgttgcccgt actcagagtc atgttgaagt gacgctggag agcggcgaga tgctgacggg
    27661 ccgcgtgctg atagccgctg atggcaccca ttcagcgtta gccaccgcgt gcggcgttga
    27721 ttggcagcag gagccttacg aacaactggc agtgattgcc aacgttgcta cttctgttgc
    27781 gcacgaaggc cgcgcttttg aacgcttcac gcaacatggc ccgctggcga tgttgccgat
    27841 gtctgacgga cgctgttcgc tggtctggtg tcatccactg gaacggcgcg aagaggtgct
    27901 gtcgtggagc gacgagaagt tttgccgtga actccagtct gcctttggct ggcgactggg
    27961 gcaaattacc cacgccggta aacgcagtgc ttatccgctg gcattaaccc gcgccgccag
    28021 accgattacc catcgcactg tgctggtggg caatgcggcg caaactctgc acccgattgc
    28081 cgggcaaggg tttaacctcg gtatgcgaga tgtcatgagc cttgcggaaa ccctgactca
    28141 ggcgcatgag cgcggagaag acatcggtga ttacggcata ttgtgccgct atcagcagcg
    28201 tcgacagagc gatcgcgaag caaccatcgg cgtcacggac agcctggtac atctttttgc
    28261 caaccgttgg gcaccgctgg ttgtcgggcg caacatcgga ctgatgacga tggaattatt
    28321 caccccggca cgcgatgtgc tggcgcagcg caccctcggt tgggtggcgc gttgaaggcg
    28381 gttttaagga ataaacaatg caaagtgttg atgtagccat tgttggcggc ggcatggtgg
    28441 ggctggcggt tgcctgcggc ttacagggga gcggcttacg cgttgccgta ctggagcagc
    28501 gcgtaccgga acctctggcg gtagatgcac taccacaact gcgcgtttcg gctatcaatg
    28561 ccgccagcga aaaattactc acccgtcttg gcgtctggca ggacattctc gctcgtaggg
    28621 ccagttgtta tcacggcatg gaagtgtggg acaaagacag ttttggtcac atctcgtttg
    28681 acgatcaaag catgggctat agccatcttg ggcacatcgt tgaaaactca gtgattcact
    28741 acgcgctgtg gaacaaagcg cagcagtcgt cagatattac tctgttggcc cccacagaat
    28801 tacagcaggt cgcctgggga gaaaatgaaa ccttcctgac gctgaaagat ggcagcatgt
    28861 taacggcgcg tctggtgatt ggcgcggacg gtgctaattc ctggttgcgc aacaaagcag
    28921 atattccgct gactttctgg gattatcagc atcacgcgct ggtagcgacc attcgcacgg
    28981 aagaaccgca tgatgcggtg gcgcggcagg ttttccatgg cgaaggcatt ctggcctttt
    29041 taccgcttag cgatccgcat ctttgctcga ttgtctggtc actgtcgcca gaggaagcgc
    29101 agcggatgca gcaggcaagt gaagacgaat ttaatcgcgc gttaaatatc gcttttgata
    29161 atcgccttgg cttatgcaag gttgagagcg cgcgtcaggt gttcccgctg acggggcgtt
    29221 atgcgcgcca gtttgccgca caccgtctgg cgctggtggg cgacgccgcg cataccattc
    29281 acccactggc ggggcagggc gttaatctcg gatttatgga cgccgcagag ctgattgctg
    29341 aactgaaacg gttgcatcgt cagggtaaag acatcgggca gtacatttac ctgcgccgct
    29401 atgaacgtag ccgcaagcac agcgcggcgc tgatgctggc tggtatgcag ggttttcgcg
    29461 atctcttttc cggcgctaat ccggcgaaaa aactgctgcg tgatattggt ttgaaactgg
    29521 ccgacacgct tcctggcgtt aagccgcaac ttatccgcca ggcaatggga ttaaacgatt
    29581 tgcctgaatg gctgcgttaa aaatttctcc tctgttgttt atttgatacc catcacactt
    29641 tcatctcccg gttttttcgc cgggagattt tcctcatttg aaataaacta atttcacctc
    29701 cgttttcgca ttatattttc taataccatg attttttgat ttagtgtttt ttgacatttt
    29761 tttagctctt aatattgtct tattcaaatt gactttctca tcacatcatc tttgtataga
    29821 aattggtgta ttttttggtt ttttattctg tcgcgatttt tgcatttttt aaccataagc
    29881 taatgtgatg atcaatttta ccttatggtt aacagtctgt ttcggtggta agttcaggca
    29941 aaagagaacg attgcgttgg ggaccgggag tggctccgat gctgggtttt gtggtgataa
    30001 tttcaccatg aaaaagttgt cagccccgct tattcaatga ggacaagatg gcacaacaga
    30061 ctcctttgta cgaacaacac acgctttgcg gcgctcgcat ggtggatttc cacggctgga
    30121 tgatgccgct gcattacggt tcgcaaatcg acgaacatca tgcggtacgt accgatgccg
    30181 gaatgtttga tgtgtcgcat atgaccatcg tcgatctccg cggcagccgc acccgggagt
    30241 ttctgcgtta tctgctggcg aacgatgtgg cgaagctcac caaaagcggc aaagcccttt
    30301 actcggggat gttgaatgcc tctggcggtg tgatagatga tctcatcgtc tactacttta
    30361 ctgaagattt cttccgcctc gttgttaact ccgccacccg cgaaaaagac ctctcctgga
    30421 ttacccaaca cgctgaacct ttcggcatcg aaattaccgt tcgtgatgac ctttccatga
    30481 ttgccgtgca agggccgaat gctcaggcaa aagctgccac actgtttaat gatgctcagc
    30541 gtcaggcggt ggaagggatg aaaccgttct ttggcgtgca ggtgggcgat ctgtttattg
    30601 ccaccactgg ttataccggt gaagcgggct atgaaattgc gctgcccaat gaaaaagcgg
    30661 ccgatttctg gcgtgcgctg gtggaagcgg gcgttaagcc ctgcggtctg ggggcgcgtg
    30721 acacgctgcg tctggaagcg ggcatgaatc tttatggtca ggagatggac gaaactattt
    30781 ctcctttagc cgccaacatg ggctggacta tcgcctggga accgacagat cgtgacttta
    30841 tcggtcgtga agccctggaa gtgcagcgtg agcatggtac agaaaaactg gttggtctgg
    30901 tgatgaccga aaaaggcgtg ctgcgtaatg aactgccggt acgctttacc gatgcgcagg
    30961 gcaaccagca tgaaggcatt atcaccagcg gtactttctc cccgacgctg ggttacagca
    31021 ttgcgctggc gcgcgtgccg gaaggtattg gcgaaacggc gattgtgcaa attcgcaacc
    31081 gtgaaatgcc ggttaaagtg acaaaacctg tttttgtgcg taacggcaaa gccgtcgcgt
    31141 gatttacttt tttggagatt gattgatgag caacgtacca gcagaactga aatacagcaa
    31201 agaacacgaa tggctgcgta aagaagccga cggcacttac accgttggta ttaccgaaca
    31261 tgctcaggag ctgttaggcg atatggtgtt tgttgacctg ccggaagtgg gcgcaacggt
    31321 tagcgcgggc gatgactgcg cggttgccga atcggtaaaa gcggcgtcag acatttatgc
    31381 gccagtaagc ggtgaaatcg tggcggtaaa cgacgcactg agcgattccc cggaactggt
    31441 gaacagcgaa ccgtatgcag gcggctggat ctttaaaatc aaagccagcg atgaaagcga
    31501 actggaatca ctgctggatg cgaccgcata cgaagcattg ttagaagatg agtaacggct
    31561 ttattcctct tctgcgggag aggatcaggg tgaggaaaat ttatgcctca ccctcactct
    31621 cttcgtaagg agagaggttc acaattcact gcacgtttca ggaaccatcg ctcatgacac
    31681 agacgttaag ccagcttgaa aacagcggcg cttttattga acgccatatc ggaccggacg
    31741 ccgcgcaaca gcaagaaatg ctgaatgccg ttggtgcaca atcgttaaac gcgctgaccg
    31801 gccagattgt gccgaaagat attcagcttg cgacaccacc gcaggttggc gcaccggcga
    31861 ccgaatacgc cgcactggca gaactcaagg ctattgccag tcgcaataaa cgcttcacgt
    31921 cttacatcgg catgggttac accgccgtgc agctaccgcc ggttatcctg cgtaacatgc
    31981 tggaaaatcc gggctggtat accgcgtaca ctccgtatca gcctgaagtc tcccagggcc
    32041 gccttgaagc actgctcaac ttccagcagg taacgctgga tttgactgga ctggatatgg
    32101 cctctgcttc tcttctggac gaggccaccg ctgccgccga agcaatggcg atggcgaaac
    32161 gcgtcagcaa actgaaaaat gccaaccgct tcttcgtggc ttccaatgtg catccgcaaa
    32221 cgctggatgt ggtccgtact cgtgctgaaa cctttggttt tgaagtgatt gtcgatgacg
    32281 cgcaaaaagt gctcgaccat caggacgtct tcggcgtgct gttacagcag gtaggcacta
    32341 ccggtgaaat tcacgactac actgcgctta ttagcgaact gaaatcacgc aaaattgtgg
    32401 tcagcgttgc cgccgatatt atggcgctgg tgctgttaac tgcgccgggt aaacagggcg
    32461 cggatattgt ttttggttcg gcgcaacgct tcggcgtgcc gatgggctac ggtggcccac
    32521 acgcggcatt ctttgcggcg aaagatgaat acaaacgctc aatgccgggc cgtattatcg
    32581 gtgtatcgaa agatgcagct ggcaataccg cgctgcgcat ggcgatgcag actcgcgagc
    32641 aacatattcg tcgtgagaaa gcgaactcca acatttgtac ttcccaggta ctgctggcaa
    32701 acatcgccag cctgtatgcc gtttatcacg gcccggttgg cctgaaacgt atcgctaacc
    32761 gcattcaccg tctgaccgat attctggcgg cgggcctgca acaaaaaggt ctgaaactgc
    32821 gccatgcgca ctatttcgac accttgtgtg tggaagtggc cgacaaagcg ggcgtactga
    32881 cgcgtgccga agcggctgaa atcaacctgc gtagcgatat cctgaacgcc gtggggatca
    32941 cccttgatga aaccaccacg cgtgaaaacg taatgcagct tttcagtgtg ctgctgggcg
    33001 ataaccacgg gctggagatc gacacgctgg acaaagacgt ggctcacgac agccgctcta
    33061 tccagcctgc gatgctgcgc gacgacgaaa tcctcaccca tccggtgttt aatcgctatc
    33121 acagcgaaac cgaaatgatg cgctatatgc actcgctgga gcgtaaagat ctggcgctga
    33181 atcaggcgat gatcccgctg ggttcctgca ccatgaaact gaacgccgcc gccgagatga
    33241 tcccaatcac ttggacggaa tttgccgaac tgcacccgtt ttgcccgccg gaacaggctg
    33301 aaggttatca gcagatgatt gcgcagctgg ctgactggct ggtgaaactg accggttacg
    33361 acgccgtttg tatgcagccg aactccggcg cacagggcga atacgcgggc ctgctggcga
    33421 ttcgtcatta tcatgaaagc cgcaacgaag ggcatcgcga tatctgcctg atcccggctt
    33481 ctgcgcacgg aactaacccc gcttctgcac atatggcagg aatgcaggtg gtggttgtgg
    33541 cgtgtgataa aaacggcaac atcgatctga ctgatctgcg cgcgaaagcg gaacaggcgg
    33601 gcgataacct ctcctgtatc atggtgactt atccttctac ccacggcgtg tatgaagaaa
    33661 cgatccgtga agtgtgtgaa gtcgtgcatc agttcggcgg tcaggtttac cttgatggcg
    33721 cgaacatgaa cgcccaggtt ggcatcacct cgccgggctt tattggcgcg gacgtttcgc
    33781 acctcaacct gcataaaact ttctgcattc cgcacggcgg tggtggtccg ggtatgggac
    33841 cgatcggtgt gaaagcgcat ctggcaccgt ttgtaccggg tcatagcgtg gtgcaaatcg
    33901 aaggcatgtt aacccgtcag ggcgcagttt ctgcggcacc gttcggtagc gcctctatcc
    33961 tgccaatcag ctggatgtac atccgcatga tgggcgcaga agggctgaaa aaagcaagcc
    34021 aggtggcaat cctcaacgcc aactatattg ccagccgcct gcaggatgcc ttcccggtgc
    34081 tgtataccgg tcgcgacggt cgcgtggcgc acgaatgtat tctcgatatt cgcccgctga
    34141 aagaagaaac cggcatcagc gagctggaca ttgccaagcg cctgatcgac tacggtttcc
    34201 acgcgccgac gatgtcgttc ccggtggcgg gtacgctgat ggttgaaccg actgaatctg
    34261 aaagcaaagt ggaactggat cgctttatcg acgcgatgct ggctatccgc gcagaaattg
    34321 accaggtgaa agccggtgtc tggccgctgg aagataaccc gctggtgaac gcgccgcaca
    34381 ttcagagcga actggtcgcc gagtgggcgc atccgtacag ccgtgaagtt gcggtattcc
    34441 cggcaggcgt ggcagacaaa tactggccga cggtgaaacg tctggatgat gtttacggcg
    34501 accgtaacct gttctgctcc tgcgtaccga ttagcgaata ccagtaattc actgattcga
    34561 gtattttcta aaggcgcttc ggcgcctttt tagtcagatg acaaagtaca aaagtgctca
    34621 gacagtcccc tcgccccttt ggggagaggg ttagggtgag gggaacaggc cggcactggc
    34681 gcgaatattt accctcaccc cgaccctctc cctgaaaggg cgagggggaa aagcgtgcca
    34741 acattgaaga ttgagccagt ttgttagcaa tctcaaagat acgtcaacga attaattttt
    34801 ctcggaaaaa caaatggcta tagcacttgt gaccggtggc agtcgcggca tcgggcgggc
    34861 aactgcatta ctgttggcgc aagaagggta tacggtggcg gttaattatc agcaaaacct
    34921 ccacgcggcg caggaagtga tgaacttaat aatgcaagcc ggtggcaaag cattcgtgct
    34981 ccaggcggat atcagcgacg aaaaccaggt cgttgcgatg tttacagcaa tcgatcagca
    35041 cgatgaaccg ctagcagcgc tggtcaataa cgccgggatc ttgtttaccc agtgcaccgt
    35101 tgaaaacctt accgcagagc gaatcaaccg agtactttcc accaacgtga cgggatattt
    35161 tctctgctgc cgcgaggcgg taaaacgcat ggcgcttaaa aatggtggca gtggcggcgc
    35221 tatcgtcaat gtctcttcgg tggcctcacg gttgggttcg ccaggggaat atgttgatta
    35281 cgcggcatcg aaaggggcga ttgattcgtt aaccaccgga ctatcgctgg aagtcgccgc
    35341 gcaggggatc cgcgttaact gcgtgcggcc agggtttatt tataccgaaa tgcacgccag
    35401 cggcggcgag cctggacgcg tcgatcgcgt taagtcgaac atccccatgc agcgtggtgg
    35461 acaggcagaa gaggtcgcgc aggccattgt ctggctacta agtgataaag cctcttacgt
    35521 cacgggaagt tttatcgatt tggcgggcgg gaaataaaac agggaagttg tctgaccgga
    35581 tgcaacaagt attgcatccg gtacttcatc gacttaaagc ttctcgccgt tgctggcaat
    35641 cacctctttg taccagttaa agctcttctt acgtgaacgc gacatatcgc cagtaccgtc
    35701 gtcatgttta ttcacataga taaagccgta gcgtttgctg tactgcccgg tagtgaatga
    35761 cacgcagtcg atgcagcccc acggtgtgta gcccatcaga tccacaccat cgtaagtcac
    35821 tgcttttttc atctcttcaa tatgggcgcg caggtagtca atgcggtagt cgtcgttgat
    35881 gctgccatct tcttccactt tgtcgtaagc gccaaaaccg ttttcgacaa taaacagcgg
    35941 cctctgataa cgctcataca gttcgcaaag ggcatagcgc agacctactg gatcaatctg
    36001 ccagccccag tcagacgctt taacatacgg gttcggtacg ctgccttcaa aaccagagat
    36061 cgcatcgccg gtgccgcctt cggccttcac tgcgttggtc atgtaatagc tgaaaccaag
    36121 ataatcgcag gtgccttcgc gcagtacatc gagatcgccg tcttccattt tgatattaaa
    36181 tccgcgacgt tcccactcgt tcaatacata ggacgggtaa tagccgcata gctgcacatc
    36241 ggtaaagacg tagcgttcgc gcatcgactc ctgagcgaac atcacatcgt ccgggttaca
    36301 tgagtaggga tagagcggca ccatcgccag catacagccg actttcatct ccgggttaat
    36361 gcgacgcgcg gctttcactg ccagggcgct ggcgacaaac tggtgatgca gcacctggta
    36421 catcgtctct tccgggttgt catgctcggt atacaccacg ccggagcagc agtaaccaaa
    36481 cagcggcgca cgccagttac gctggttgtt tatttcgttg aaggtcatcc agtatttgac
    36541 tttatgcttg tagcgttcga ataccacttc cgcgaaacgg acaaagaaat caacgaattt
    36601 acggttggtc cagctaccgt attgctgcac cagatgcagc ggcatttcaa agtgggagag
    36661 ggttatcacc ggttcgatgt tgtatttcag cagttcatcg aacagagagt cgtaaaactt
    36721 cagcccttct tcgtttggct gagcttcatc accttttgga aaaatacgcg tccaggcgat
    36781 ggaagtccga aaacatttga agcccatttc ggcaaatagc ttgatgtctt ccttgtagtg
    36841 accataaaaa tcaacggctt catggtttgg atagtatttt cctggcaaga cttctttggt
    36901 gatttcgcgc ggcacgccgt gtgcgccaca ggtcagaacg tcacaaatgc tcggtccttt
    36961 gccgcctttg ttccagccgc cttcgacctg atgagcggca acggcgccac cccataagaa
    37021 atctttcggt aaggtgagtt ttttcactat cataggctcc agatcagtca attcttacag
    37081 gaaatagtac catgcagcca aacgacatca cttttttcaa cgtttccagg atgacattct
    37141 ggctgggcgt aaaaccatca ccatccgcga cgagtctgaa tcgcacttca aaacgggtga
    37201 tgtgcttcgt gtcggacgtt ttgaagatga cggttatttt tgcacgattg aagtcaccgc
    37261 aacctcaacc gtaacgctgg atacactgac ggaaaaacat gcagagcagg aaaatatgac
    37321 cctgcctgaa ctgaaaaagg tcattgctga catctatccc ggtcagacac aattttatat
    37381 gattgaattt aaatgtcttt aaattgaact cacgcctgtt agttgaaatt tcgcatttaa
    37441 tttcatgatt tttaggagat tagtctcaaa tcagaattta ttttagctaa caggtgttca
    37501 ctggaactat tctcagttac gctggagagg tatacatgtg cgtgtacgtt tctctggagt
    37561 aagttatggt tcagaagccc ctcattaagc agggatattc gctggcagag gaaattgcca
    37621 acagcgtcag tcacggcatt gggctggtgt ttggtatcgt tgggctggtg ttgctactgg
    37681 ttcaggcggt ggatctcaat gccagcgcca cagcgataac cagttacagc ctctatggcg
    37741 gcagtatgat cctgctgttc ctcgcttcga cgctctatca cgccattcct catcaacggg
    37801 caaaaatgtg gctgaagaaa tttgaccatt gcgctattta cctgttgatt gccggaacct
    37861 acacgccgtt tttgctggtg gggctggatt ctccgttagc gcgcgggttg atgattgtta
    37921 tctggagcct agcattgctg gggattctgt tcaaactgac catcgcgcac cgattcaaaa
    37981 ttttatctct ggtgacttat ctggcgatgg gctggctgtc gctggtggta atttatgaaa
    38041 tggcagttaa gctcgcggcg ggcagcgtta ccttactggc ggtaggcggc gtggtttatt
    38101 cgctcggggt gattttctac gtctgcaaac gcattccata caaccatgcc atctggcacg
    38161 gcttcgtgct cggcggtagt gtgtgccact ttctggcgat ctatttgtat attgggcagg
    38221 cgtaatgggt atgcctttgt aggcctgata agacgcgtca gcgtcgcatc aggcatcgga
    38281 gcgcaaccgc cggatgcggc gtgaacgcct tatccgatct acgggcctcg tccctgttgt
    38341 gataaaggca tcaaatccca gacatgataa gacgcgtcag cgtcgcatca ggcgataaat
    38401 gcctgatgcg atacagagat ttactcttcg agcgaatacg gcagcggctc gatatgcagt
    38461 gtattcgcat cgtcgcgtac gcggaagatg ctgtccggtt ccatatcgtt attcatgacg
    38521 acctgtacca cgacctgacc atcttccagt tttaccgcag ccagcaccgt accggtacga
    38581 cgccagttct cgcccatttt cagctctaag tcttcaccag cttccggcag tcggctggca
    38641 ctacctgcca gcaaccagag cgcgcgtttg ttggcaccac ggaattttgc tcgcgccacc
    38701 atctcttgtc cggtataaca gcctttctta aagctgatac cgcccagcgc ctggagattg
    38761 gtcgcctgtg ggataaactg cccgctgttg gcggcatcaa tcaccgggaa accagcttca
    38821 atgtttaatg ccagccactg ttggctattg ttcagttccg cttcaccgcg cagtttatcg
    38881 gtcagcatat tagcagtagc ttcatcggtt acgatcagga aacgttctgc cgggtgttca
    38941 aaccatagca aagtggtcgc gccttctttg actacctgtt tttctttcga aggcagttcg
    39001 ctaaagagat ttgccagcgc ggcgcgcgcc tgaaaaccgg caacaccaag cagcacacgc
    39061 tcgtcgtctg gcgcgatggt cactttagag aataccgcat attttttcag ttcagtcagc
    39121 tgcggttcac gcacgctgcg ccgttcaatc catgcaaagc catcgccgtc gcggaacaga
    39181 cgtaaattgc tccacatttt acctttggcg tcgcaatggg cggcgagcag gtgctgatct
    39241 tctgccatct ggctgacatc tgctgtcacc tgaccctgca tatatttttc gctgtccgcg
    39301 ccagtaatgg tggcaagcgc ccagtcatca agcgtcatca gcgtcagcgg taaacgggca
    39361 gaagccgtag gctgacgtgg aggaaaaggt gtaaaagcca taatagagtc ctgagttgct
    39421 taacggcgta ttaatgacta atggtaaaag agctatgagg caatgcaagt gctttcacta
    39481 cgcgatctgt gccttctgcg aggcgactgc caaaaaaatg aagcccttga attgttgtga
    39541 attaatttcg cgatcgcgat ggtgtttact gatattcccg taaaagaagt cgaaaaacac
    39601 gttacaatag ccgggtactg gattttcgtg agacaggaag aagatggaca ttaacaacaa
    39661 agcccgaatt cattgggcat gccgccgtgg tatgcgcgaa ctcgatattt caatcatgcc
    39721 gtttttcgaa catgagtacg acagcttaag cgatgacgaa aaacgcatct ttattcgtct
    39781 gctggaatgt gacgatccgg acctgtttaa ctggctgatg aatcacggta aaccggccga
    39841 tgcagaactg gaaataatgg tccgactcat ccagacacgg aaccgggaac gtggtcctgt
    39901 ggcaatctga tttgcgcgtc tcctggcgcg cacagtggct ttccttgctg attcatgggc
    39961 tggttgccgc tgttatttta ctcatgccct ggccgctcag ttacaccccg ttatggatgg
    40021 tgttactttc gctggtggta tttgattgcg ttcgcagcca gcggtgtatt aatgctcgcc
    40081 agggggaaat tcgcttgttg atggacgggc gtttgcgttg gcaagggcag gagtggagca
    40141 tcgtcaaagc accgtggatg attaagagcg gcatgatgct gcgtttacgt tctgatggcg
    40201 gtaaacggca acatttatgg ctggcagccg acagcatgga cgaagccgaa tggcgggatt
    40261 tacggcggat tttgttgcaa caagagacgc aaagataaag atgacggcaa agcatatgcg
    40321 ctaagccgtc agtgagatca ggcgtaatgc tctgccattt cgttgaggat ttgctcgcac
    40381 cagctctgaa tacgctcgtc gctaaggtca tactggttag tttcatccag cgccagaccc
    40441 acgaacagtt gcccgtcagc aatcaccggt ttcgggctgg taaattcata tccttccgtt
    40501 ggccagtagc cgacgaactt cacgcctttg gtcgagagtt tgtcatgcag cataccgagc
    40561 gcatcgagga accactcgcc gtatcccagt tgatcgccaa gcccatacag cgcaataatt
    40621 ttaccttcaa ggttcaggtc gtcgagctga tcccagacgg cttcccagtc ttcctggatt
    40681 tcaccaaaat cccaggtcgg gatacccaga atgagcacat cgtactgctc cattaatttc
    40741 ggggagtcgt ccttgaggtt atgtaaggtc accagttctg ggccgataat atcgcggatt
    40801 ttttctgccg ccatttcggt gtaacaggtg ctggaaccgt aaaaaagacc catattcata
    40861 acgtaagctt ctcgattctg ctgtggcttt tgcagctagt gtatcagaat cgctttagtc
    40921 acggcacaat aaggcataat ggatcaaatg agtgaccata aggggcgcaa gtgaaacagg
    40981 agctggcacg catcgagcag tttcttgatg ctctgtggct ggaaaaaaat ctggctgaaa
    41041 atacgttgaa cgcttaccgt cgcgatctgt caatgatggt ggagtggttg catcaccgcg
    41101 ggttgacgct ggcgacggcg caaagtgacg atttgcaggc attactggca gaacggctgg
    41161 agggcgggta taaagccacc agctcagcgc gtttgctgag tgcagtgcgc cgattgttcc
    41221 agtatcttta tcgcgaaaag tttcgtgaag acgatcccag tgcgcatctc gcttcaccga
    41281 aattgcccca gcgtttgcca aaagatttaa gcgaagcgca ggtcgaacgt ttattacagg
    41341 cgccattaat tgatcagcca ctggagctac gcgataaagc catgcttgaa gtgttgtatg
    41401 ctaccgggct gcgtgtctct gaactggtcg ggctgacaat gagtgatatc agcctgcgtc
    41461 agggcgtagt gcgggtcatt ggtaaaggca acaaagagcg cctggtgccg ttaggggaag
    41521 aggcggttta ctggctggaa acctatctgg aacatgggcg tccgtggctg ctgaatggtg
    41581 tgtcaattga cgtgttgttt cccagccagc gtgcgcagca gatgacgcga cagaccttct
    41641 ggcaccgcat taaacattat gccgtgctgg cgggtatcga cagcgaaaag ctgtcaccgc
    41701 atgtgttgcg tcacgctttt gccactcatt tattaaatca tggtgcggat ttacgcgtgg
    41761 tgcagatgct actgggccac agcgatcttt ccaccacgca aatttatacg catgtcgcta
    41821 ccgagcgtct gcggcaactt catcaacagc atcacccgcg ggcgtgatgt ctgaaaagaa
    41881 cgggaagatt tatgaagaaa ggttttatgt tgtttacttt gttagcggcg ttttcaggct
    41941 ttgctcaggc tgatgacgcg gcaattcaac aaacgttagc caaaatgggc atcaaaagca
    42001 gcgatattca gcccgcgccc gtagctggca tgaagacagt tctgactaac agcggtgtgt
    42061 tgtacatcac cgatgatggt aaacatatca ttcaggggcc aatgtatgac gttagtggca
    42121 cggctccggt caatgtcacc aataagatgc tgttaaagca gttgaatgcg cttgaaaaag
    42181 agatgatcgt ttataaagcg ccgcaggaaa aacacgtcat caccgtgttt actgatatta
    42241 cctgtggtta ctgccacaaa ctgcatgagc aaatggcaga ctataacgcg ctggggatca
    42301 ccgtgcgtta tcttgctttc ccgcgccagg ggctggacag cgatgcagag aaagaaatga
    42361 aagctatctg gtgtgcgaaa gataaaaaca aagcgtttga tgatgtgatg gcaggtaaaa
    42421 gcgtcgcacc agccagctgc gacgtggata ttgccgacca ttacgcactt ggcgtccagc
    42481 ttggcgttag cggtactccg gcagttgtgc tgagcaatgg cacacttgtt ccgggttacc
    42541 agccgccgaa agacatgaaa gaattcctcg acgaacacca aaaaatgacc agcggtaaat
    42601 aattcgcgtg aaacaacaga tacaacttcg tcgccgtgaa gtcgatgaaa cggcagactt
    42661 gcccgctgaa ttgcctccct tgctgcgccg tttatacgcc agccggggag tacgcagtgc
    42721 gcaagaactg gaacgcagtg ttaaaggtat gttgccctgg cagcaactaa gcggtgtcga
    42781 aaaggccgtt gagatccttt acaacgcttt tcgcgaagga acgcggatta ttgtggtcgg
    42841 tgatttcgac gccgacggcg cgaccagcac ggctctaagc gtgctggcga tgcgctcgct
    42901 tggttgcagc aatatcgact acctggtacc aaaccgtttc gaagacggtt acggcttaag
    42961 cccggaagtg gtcgatcagg cccatgcccg tggcgcgcag ttaattgtca cggtggataa
    43021 cggtatttcc tcccatgcgg gagttgagca cgctcgctcg ttgggcatcc cggttattgt
    43081 taccgatcac catttgccgg gcgacacatt acccgcagcg gaagcgatca ttaaccctaa
    43141 cttgcgcgac tgtaatttcc cgtcgaaatc actggcaggc gtgggtgtgg cgttttatct
    43201 gatgctggcg ctgcgcacct ttttgcgcga tcagggctgg tttgatgagc gcggcatcgc
    43261 aattcctaac ctggcagaac tgctggatct ggtagcactg gggacagtag cggacgtcgt
    43321 gccgctggac gcgaataatc gcattctgac ctggcagggg atgagtcgca tccgtgccgg
    43381 aaagtgccgt ccagggatta aagcgctgct ggaagtggca aatcgcgatg cacaaaaact
    43441 agccgccagc gatttaggtt ttgcgctggg accgcgtctc aatgctgccg ggcgactgga
    43501 tgatatgtcc gttggtgtgg cgctgttgct gtgcgacaac atcggcgaag cgcgcgtgct
    43561 ggcaaatgaa ctcgatgcgc taaaccagac gcgaaaagag atcgaacaag gaatgcaggt
    43621 tgaagccctg accctgtgcg agaaactgga gcgcagccgt gacacgctac ccggcgggct
    43681 ggcaatgtat caccccgaat ggcatcaggg cgttgtcggt attctggctt cgcgtatcaa
    43741 agagcgtttt caccgtccgg ttatcgcctt tgcgccagca ggtgacggta cgctgaaagg
    43801 ttccggtcgc tccattcagg ggctgcatat gcgtgatgcg ctggagcgat tagacacact
    43861 ctaccccggc atgatgctca agtttggcgg tcatgcgatg gcggcgggtt tgtcgctgga
    43921 agaggataaa ttcgaactct ttcaacaacg gtttggcgaa ctggttactg agtggctggc
    43981 cccttcgcta ttgcaaggcg aagtggtgtc agacggtccg ttaagcccgg ccgaaatgac
    44041 catggaagtg gcgcagctgc tgcgcgatgc tggcccgtgg gggcagatgt tcccggagcc
    44101 gctgtttgac ggtcatttcc gtctgctgca acagcggctg gtgggcgaac gtcatttgaa
    44161 ggtgatggtc gaaccggtcg gcggcggtcc actgctggat ggtattgctt ttaatgtcga
    44221 taccgctctc tggccggata acggcgtgcg cgaagtgcaa ctggcttata agctcgatat
    44281 caacgagttt cgcggcaacc gcagcctgca aattatcatc gacaatatct ggccaattta
    44341 gcgtcatctt ctctataaaa aagagcgtgg attgggtaca atcccgctct tatcaccgca
    44401 ttttgactag ctcaataaaa gaaatcagac catgtttgaa attaatccgg taaataatcg
    44461 cattcaggac ctcacggaac gctccgacgt tcttaggggg tatctttgac tacgacgcca
    44521 agaaagagcg tctggaagaa gtaaacgccg agctggaaca gccggatgtc tggaacgaac
    44581 ccgaacgcgc acaggcgctg ggtaaagagc gttcctccct cgaagccgtt gtcgacaccc
    44641 tcgaccaaat gaaacagggg ctggaagatg tttctggtct gctggaactg gctgtagaag
    44701 ctgacgacga agaaaccttt aacgaagccg ttgctgaact cgacgccctg gaagaaaaac
    44761 tggcgcagct tgagttccgc cgtatgttct ctggcgaata tgacagcgcc gactgttacc
    44821 tcgatattca ggcggggtct ggcggtacgg aagcacagga ctgggcgagc atgcttgagc
    44881 gtatgtatct gcgctgggca gaatcgcgtg gtttcaaaac tgaaatcatc gaagagtcgg
    44941 aaggtgaagt ggcgggtatt aaatccgtga cgatcaaaat ctccggcgat tacgcttacg
    45001 gctggctgcg tacagaaact ggcgttcacc gcctggtgcg taaaagcccg tttgactccg
    45061 gcggtcgtcg ccacacgtcg ttcagctctg cgtttgttta tccggaagtt gatgatgata
    45121 ttgatatcga aatcaacccg gcggatctgc gcattgacgt ttatcgcgcg tccggcgcgg
    45181 gcggtcagca cgttaaccgt accgaatctg cggtgcgtat tacccacatc ccgaccggga
    45241 tcgtgaccca gtgccagaac gaccgttccc agcacaagaa caaagatcag gccatgaagc
    45301 agatgaaagc gaagctttat gaactggaga tgcagaagaa aaatgccgag aaacaggcga
    45361 tggaagataa caaatctgac atcggctggg gcagccagat tcgttcttat gtccttgatg
    45421 actcccgcat taaagatctg cgcaccgggg tagaaacccg caacacgcag gccgtgctgg
    45481 acggcagcct ggatcaattt atcgaagcaa gtttgaaagc agggttatga ggaaccaaca
    45541 tgtctgaaca acacgcacag ggcgctgacg cggtagtcga tcttaacaat gaactgaaaa
    45601 cgcgtcgtga gaagctggcg aacctgcgtg agcagggaat tgccttcccg aacgatttcc
    45661 gtcgcgatca tacctctgac caattgcacg cagaattcga cggtaaagag aacgaagaac
    45721 tggaagcgct gaacatcgaa gtcgccgttg ctggccgcat gatgacccgc cgtattatgg
    45781 gtaaagcgtc tttcgttacc ctgcaggatg tgggtggccg cattcagctg tacgttgccc
    45841 gtgacgatct gccggaaggc gtttacaacg agcagttcaa aaaatgggac ctcggcgaca
    45901 tcctcggcgc gaaaggtaaa ctgttcaaaa ccaaaaccgg cgaactgtct atccactgta
    45961 ccgagctgcg tctgctgacc aaagcactgc gtccgctgcc ggataaattc cacggcttgc
    46021 aggatcagga agcgcgctat cgtcagcgtt atctggatct catctctaac gatgaatccc
    46081 gtaacacctt taaagtacgc tcgcagatcc tctctggtat tcgccagttc atggtgaacc
    46141 gcggctttat ggaagttgaa acgccgatga tgcaggtgat cccaggcggt gccgctgcgc
    46201 gtccgtttat cacccaccat aacgcgctgg atctcgacat gtacctgcgt atcgcgccgg
    46261 aactgtacct caagcgtctg gtggtcggtg gcttcgagcg tgtattcgaa atcaaccgta
    46321 acttccgtaa cgaaggtatt tccgtacgtc ataacccaga gttcaccatg atggaactct
    46381 acatggctta cgcagattac aaagatctga tcgagctgac cgaatcgctg ttccgtactc
    46441 tggcacagga tattctcggt aagacggaag tgacctacgg cgacgtgacg ctggacttcg
    46501 gtaaaccgtt cgaaaaactg accatgcgtg aagcgatcaa gaaatatcgc ccggaaaccg
    46561 acatggcgga tctggataac ttcgactctg cgaaagcgat tgctgaatct atcggtatcc
    46621 acgttgagaa gagctggggt ctgggccgta tcgttaccga gatcttcgaa gaagtggcag
    46681 aagcacatct gattcagccg accttcatta ctgaatatcc ggcagaagtt tctccgctgg
    46741 cgcgtcgtaa cgacattaac ccggaaatca cagaccgctt tgagttcttc atcggtggtc
    46801 gtgaaatcgg taacggcttt agcgagctga atgacgcgga agatcaggcg caacgcttcc
    46861 tggatcaggt tgccgcgaaa gacgcaggtg acgacgaagc gatgttctac gatgaagatt
    46921 acgttaccgc actggaacat ggcttaccgc cgacagcagg tctggggatt ggcatcgacc
    46981 gtatggtaat gctgttcacc aacagccata ccatccgcga cgttattctg ttcccggcga
    47041 tgcgtccggt gaaataagca ttacgttatg ctcacaaccc cggcaaatgt cggggttttt
    47101 tatttaagct gggtaaatgc agataatcgt attctggctt cgcgatttgt cgcctgcatc
    47161 accatccacg gactgaacgc ccacggcgtg gcatcaatac cgcgtaatac atcagctaaa
    47221 tcacaccatt gataatccat cacttcatca tcgttgatct gtaacgcact ggtggtgcgt
    47281 gcggcaaata ccggacacac ttcattttcc acaatgcctc tcggatcggt ggcgcggtag
    47341 cgaaagtcag gatagataga ttcaggaggc gtaatttcca cgccaagctc ataacggcaa
    47401 cggcggatca ccgcgtcttc gctgctttct cccagttgtg ggtgcccaca aaccgagtta
    47461 gtccacacgc caggccacgc ttttttgctc agtgcgcggc gggtaactaa taattgcccc
    47521 ttggcattaa acagccaact ggagaacgcg agatgtaaga gggtgtctgc cgtgtgtgcg
    47581 gcatactttt ccagcgtacc cgtgggaact ccctgtgcat tcaataaaat gacgtgttcc
    47641 gtttgcataa tttctcacat gtaattctga tcactcatca ttatagataa ttgaagagtg
    47701 aaatgattaa ccaagagagc gtttatcgac aaccgcccgt tttgagcgga tgccgacagg
    47761 taaaaaccat ttaaaaaagt agcatcatat atttgaagcg actttcaatg gccattgcaa
    47821 cacagtcaga aatgacagga atagagagag taaaaagcaa caacagactg aatatagcgc
    47881 gcattaaaaa attcatatta ttctccatgc tcatttttct tcagcttgcg catcagaagc
    47941 cacatacgta ctgtcctgac ttctaaatct ttatcagaga cggattcctt caccaggtca
    48001 gcatgatgct gatagccatt aaagaagacg ttcagcacaa cggcgctaag ggttgcgagc
    48061 ataatgccgc tatgtagcag cggttgcagt acggccggta acttagaaaa gaaatcgtga
    48121 gagagcgtcg gagtcatgcc aacgccgaga ctgatcgcca caatatagag gttgtaacgg
    48181 ttggtggtgt agttacagcg cgacagaatt cgaatccctg tcgccagtac catgccgaac
    48241 atcactagac cagcgccgcc cagcacaaat tgcggaatgg aggctaccag caccgccatt
    48301 tttggcacca tgccaaacag gattaatata attcccgaag aaatacacac ccagcggcta
    48361 tgaacgcgca tcacgctaac caggccaacg ttttgagaaa atgacgtgtg ggggaagctg
    48421 ttaaacgtgc cgcctatcat tgtccctacg ccatcgacac gcagcccgca aataatatcg
    48481 tgtgaagaga gtttacgacc gactatttca cccagtgcca ggaacatccc catcgactcg
    48541 ataaacacga tgattaacac ggcagtcatg gtcagaatgg aaacgggatc gaaaatcggc
    48601 ataccaaacg acatcggggt gacaatcgca aaccacgagg catcatgtag cccggataaa
    48661 ttgacttcat tcatcatcca cgaaagtaaa aagccaaata caatccccag taatacggcg
    48721 acgttggaca taaatccttt cgcatagcga gtaatgagca agataaaaat taagacggca
    48781 aaggagatac ctaaataaac gggattacca tattgcggat tccctttacc tccagcggcc
    48841 cagtcaatac ccacctgaat gatgctaagc ccaatagaag taataaccac accggtaacc
    48901 agtggcggga ataaaggcat caagcgacca ataagtggcg ctaataatgt ggtgataaaa
    48961 cccgcggcga tagtggcacc aaatatcccc agcaggccga tatccgggtt catcccaatg
    49021 gctatcattg gtgttacagc agcaaaggtc accgacataa tcaccggcag gcggatcccc
    49081 ataaagcggc cgataccgat acattgcaat aatgtgacga tcccgcagca aaagagatcc
    49141 gagctaatga gcatcgcaat agcatctttt gagaggccca gtcggtcacc aatcataaga
    49201 ggaacagcga ctgcacctgc gtacatgaca aggacgtgtt gcagaccgag tatgatcagc
    49261 tttcctggtg ataatatgcg gtcaacctca tcagttgggc ggtcttgccc agaagatgag
    49321 ggaagttggg aatctatggc gctcatcagt gtttctcctt tcgcccgcca gaataaaaag
    49381 caaccgttta cgttaatgaa actgtctaaa cacatgctcg tggagtgcca gtttttgcga
    49441 ggaaggggag ttagagacag gaattgcgga ggcaatcaca taatcgtgct taaaccacga
    49501 aaatgacaag agaaactgac aatggaatta tctgtaattt tctcatattg atatttcatt
    49561 aatcattctg gcatgtcgca ctctcgcatt taatcgtttt tatctggata gcgctctttt
    49621 gatcggcaat gttgaaataa tgaaagcggc atagattgag gggcaataat ttgaactcag
    49681 tcgcaaaatt ataattaaaa cggtcatgct acctaatata tgcccgttta ttgcatcgcg
    49741 aaatttgaga agacataact cattaaatga aagggatgca aatgaatacg tttatcgctg
    49801 ctgaagctgc ggaatgta
//
LOCUS       DAJTRD010000021        49530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTRD010000021 DAJTRD010000000
VERSION     DAJTRD010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.021"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..1151)
                     /gene="setB"
                     /locus_tag="OO315_001333"
     CDS             complement(<1..1151)
                     /gene="setB"
                     /locus_tag="OO315_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5890310.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATL"
     gene            1519..2649
                     /gene="fruB"
                     /locus_tag="OO315_001334"
     CDS             1519..2649
                     /gene="fruB"
                     /locus_tag="OO315_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5890311.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2649..3587
                     /gene="fruK"
                     /locus_tag="OO315_001335"
     CDS             2649..3587
                     /gene="fruK"
                     /locus_tag="OO315_001335"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5890312.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3604..5295
                     /gene="fruA"
                     /locus_tag="OO315_001336"
     CDS             3604..5295
                     /gene="fruA"
                     /locus_tag="OO315_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5890313.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5719..6660
                     /locus_tag="OO315_001337"
     CDS             5719..6660
                     /locus_tag="OO315_001337"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5890314.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6648..7586
                     /gene="psuG"
                     /locus_tag="OO315_001338"
     CDS             6648..7586
                     /gene="psuG"
                     /locus_tag="OO315_001338"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5890315.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7680..8930
                     /locus_tag="OO315_001339"
     CDS             7680..8930
                     /locus_tag="OO315_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5890316.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9001..9695)
                     /gene="yeiL"
                     /locus_tag="OO315_001340"
                     /pseudo
     CDS             complement(9001..9695)
                     /gene="yeiL"
                     /locus_tag="OO315_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9826..10767
                     /gene="rihB"
                     /locus_tag="OO315_001341"
     CDS             9826..10767
                     /gene="rihB"
                     /locus_tag="OO315_001341"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5890317.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            10867..12117
                     /locus_tag="OO315_001342"
     CDS             10867..12117
                     /locus_tag="OO315_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5890318.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12173..13261)
                     /locus_tag="OO315_001343"
     CDS             complement(12173..13261)
                     /locus_tag="OO315_001343"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5890319.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13264..14121)
                     /gene="nfo"
                     /locus_tag="OO315_001344"
     CDS             complement(13264..14121)
                     /gene="nfo"
                     /locus_tag="OO315_001344"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5890320.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14195..15244)
                     /locus_tag="OO315_001345"
     CDS             complement(14195..15244)
                     /locus_tag="OO315_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5890321.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15343..16224
                     /locus_tag="OO315_001346"
     CDS             15343..16224
                     /locus_tag="OO315_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5890322.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16430..17899
                     /gene="lysP"
                     /locus_tag="OO315_001347"
     CDS             16430..17899
                     /gene="lysP"
                     /locus_tag="OO315_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5890323.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18191..20182
                     /gene="cirA"
                     /locus_tag="OO315_001348"
                     /pseudo
     CDS             18191..20182
                     /gene="cirA"
                     /locus_tag="OO315_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20214..21049)
                     /gene="yeiG"
                     /locus_tag="OO315_001349"
                     /pseudo
     CDS             complement(20214..21049)
                     /gene="yeiG"
                     /locus_tag="OO315_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21307..21975
                     /gene="folE"
                     /locus_tag="OO315_001350"
     CDS             21307..21975
                     /gene="folE"
                     /locus_tag="OO315_001350"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5890324.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            21992..23068
                     /locus_tag="OO315_001351"
     CDS             21992..23068
                     /locus_tag="OO315_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5890325.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23210..24250
                     /gene="galS"
                     /locus_tag="OO315_001352"
     CDS             23210..24250
                     /gene="galS"
                     /locus_tag="OO315_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5890326.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24530..25528
                     /gene="mglB"
                     /locus_tag="OO315_001353"
     CDS             24530..25528
                     /gene="mglB"
                     /locus_tag="OO315_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5890327.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25589..27109
                     /gene="mglA"
                     /locus_tag="OO315_001354"
     CDS             25589..27109
                     /gene="mglA"
                     /locus_tag="OO315_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5890328.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27125..28135
                     /gene="mglC"
                     /locus_tag="OO315_001355"
     CDS             27125..28135
                     /gene="mglC"
                     /locus_tag="OO315_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5890329.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28171..29384)
                     /gene="preA"
                     /locus_tag="OO315_001356"
                     /pseudo
     CDS             complement(28171..29384)
                     /gene="preA"
                     /locus_tag="OO315_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29378..30616)
                     /gene="preT"
                     /locus_tag="OO315_001357"
     CDS             complement(29378..30616)
                     /gene="preT"
                     /locus_tag="OO315_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5890330.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30810..31049)
                     /locus_tag="OO315_001358"
     CDS             complement(30810..31049)
                     /locus_tag="OO315_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5890331.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31052..31771)
                     /gene="sanA"
                     /locus_tag="OO315_001359"
     CDS             complement(31052..31771)
                     /gene="sanA"
                     /locus_tag="OO315_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5890332.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(31921..32805)
                     /gene="cdd"
                     /locus_tag="OO315_001360"
     CDS             complement(31921..32805)
                     /gene="cdd"
                     /locus_tag="OO315_001360"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5890333.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(32935..33630)
                     /locus_tag="OO315_001361"
     CDS             complement(32935..33630)
                     /locus_tag="OO315_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5890334.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33627..34025)
                     /locus_tag="OO315_001362"
     CDS             complement(33627..34025)
                     /locus_tag="OO315_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5890335.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34264..35211
                     /gene="dusC"
                     /locus_tag="OO315_001363"
     CDS             34264..35211
                     /gene="dusC"
                     /locus_tag="OO315_001363"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5890336.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35582..35665)
                     /gene="yohP"
                     /locus_tag="OO315_001364"
     CDS             complement(35582..35665)
                     /gene="yohP"
                     /locus_tag="OO315_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5890337.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            35889..37325
                     /gene="mdtQ"
                     /locus_tag="OO315_001365"
                     /pseudo
     CDS             35889..37325
                     /gene="mdtQ"
                     /locus_tag="OO315_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37378..38139
                     /locus_tag="OO315_001366"
                     /pseudo
     CDS             37378..38139
                     /locus_tag="OO315_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38256..38834)
                     /locus_tag="OO315_001367"
     CDS             complement(38256..38834)
                     /locus_tag="OO315_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5890338.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39004..39591
                     /locus_tag="OO315_001368"
     CDS             39004..39591
                     /locus_tag="OO315_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5890339.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39765..40697
                     /gene="pbpG"
                     /locus_tag="OO315_001369"
     CDS             39765..40697
                     /gene="pbpG"
                     /locus_tag="OO315_001369"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5890340.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40735..42450)
                     /gene="dld"
                     /locus_tag="OO315_001370"
     CDS             complement(40735..42450)
                     /gene="dld"
                     /locus_tag="OO315_001370"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5890341.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42646..44943
                     /gene="bglX"
                     /locus_tag="OO315_001371"
     CDS             42646..44943
                     /gene="bglX"
                     /locus_tag="OO315_001371"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5890342.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45195..46112
                     /gene="osmF"
                     /locus_tag="OO315_001372"
     CDS             45195..46112
                     /gene="osmF"
                     /locus_tag="OO315_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5890343.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46119..47276
                     /gene="yehY"
                     /locus_tag="OO315_001373"
     CDS             46119..47276
                     /gene="yehY"
                     /locus_tag="OO315_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5890344.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47269..48195
                     /gene="yehX"
                     /locus_tag="OO315_001374"
     CDS             47269..48195
                     /gene="yehX"
                     /locus_tag="OO315_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5890345.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48200..48931
                     /gene="yehW"
                     /locus_tag="OO315_001375"
     CDS             48200..48931
                     /gene="yehW"
                     /locus_tag="OO315_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5890346.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(48912..49019)
                     /locus_tag="OO315_001376"
     CDS             complement(48912..49019)
                     /locus_tag="OO315_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5890347.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(49079..49530)
                     /locus_tag="OO315_001377"
                     /pseudo
     CDS             complement(49079..49530)
                     /locus_tag="OO315_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
BASE COUNT        11956 a        12203 c        13311 g        12060 t
ORIGIN      
        1 agagtggcga taatcatcac catcgcaaac cagaacacag cgtgataatt ccagatctcg
       61 gcgacgatgc ccgccactga tcctgcgatg atccagccca cgcgcgacgt gttggtatag
      121 agcgtggtgg ctgaacctgc ctgaccgggc atcaaatcct gaaaatagag catcccgatg
      181 ccgcccagaa tgccaataaa aatagcattt agcagctgca agcccaacag aatggcaggt
      241 gaatgcgcca tcagcattcc tgcgtaaaaa cagacgccac ccacggcagc aacgcgcatt
      301 aagaaacgct tacccagacg tttggcgaaa tatccggcaa tcaacatcgt cgggatttcc
      361 agcccggcgg cggtccccat catcacaccg gccagtttct cgggaagatg cagttcgttg
      421 ataataaata gcggcatgtt gatgatgtac aggctgttcg agccccacat caatgtacaa
      481 atgacaaaca gcagtagcgt atcgcgacgg ttacggcgcg gcgcttcaac cgtgccggtc
      541 gccagcggaa gctctttttg catcgacggt aaaaacagcc acaccatcac gccgcaaaca
      601 ataaacgcta ccgctgcgct cagatacatt accgtaaagc tgaaacccat cgctaacgca
      661 taagccagcg gtgggccaat gacccatgcc agtgaaacct gagcgcgtaa aaaagagctg
      721 aacatcaccg cctcacgtcc ggttttgtcg gcatgttcac gggcaagggc aaacatttgc
      781 gggttagcgg tcgagccaaa gctgctaaga aagacgccaa cgaatagcaa aacaaagtag
      841 ttgcgattcc aagcaaaaag ggtgcaggcc agcacgccta acaggcagca aaagacaatc
      901 agcgatttgc gatcgccgcg cttatcagag cgcccagcga gaaactgact taccagaatc
      961 ccaatgacag cgctgccggt aaagaagaat cccaccatcg ccggacgggc atgtacttca
     1021 tcggtaagga aaatactgag tgtcggggtt tgcagagcac ccgcaatacc ggtgagaaag
     1081 gcaacgatta aaaacgccgt cgaggtcagg tcaaacgatt tcgcgctgga gactgcgggg
     1141 gagttatgca tgctggtcga tcactggaag aggaagacgc ggagtttacg ctgcttgtca
     1201 gaaaataaac agacagcgaa atcaaaagcg ccacgaataa tcaaaatgcc atttcactgt
     1261 tgctgattgg ctgctgacgc tgctgaaatg aaattgctga taacggattt tcccatcagc
     1321 aattaggaaa aatgacaaaa aattgtgcag cacatcaaac ttttgctcat aactttacgg
     1381 ctttccttgc gtgctgaaac agaagtatta tgctttcttg aaacgtttca gcgcgatctt
     1441 gtctttaacc ctaagctagg ttggcgcttt ttttctcata gaggctgaat cgtttcaatt
     1501 cagcaagaga ggagaactat gttccagtta tccgtacagg acatccatcc gggcgaaaag
     1561 gccggagaca aagaagaggc gattcgccag gtcgctgcgg cgctggtgca ggccggtaat
     1621 gtagcagaag gctacgtcaa tggcatgctg gcgcgcgaac agcaaacctc aacgttcctc
     1681 ggcaatggta ttgctattcc acacggcact accgacaccc gcgatcaggt gctgaaaacc
     1741 ggcgttcagg tatttcagtt cccggaaggc gtcacctggg gtgacggtca ggtagcgtac
     1801 gtggcaatcg gtattgctgc cagctcggat aaacatctgg gcctgctacg ccagctgacc
     1861 cacgtactga gcgatgattc cgttgctgaa caactgaagt cagcaacaac agcagaagaa
     1921 cttcgcgcat tactgatggg cgaaaagcag agtgagcagc tgaagctcga caacgaaatg
     1981 ctgacgctgg atatcgtcgc cagcgatctg ctgactcttc aggcgctgaa cgctgcgcgt
     2041 ctgaaagagg cgagggcagt tgacgccact ttcgtcacca aagccatcaa tgaacaaccg
     2101 ctgaatctcg gacagggtat ctggctgagc gatagcgccg aaggcaatct gcgtagtgcg
     2161 attgcggtaa gccgtgcggc aaatgctttt gatgtggacg gcgaaacggc agccatgctg
     2221 gtgagtgtgg cgatgaatga cgatcagccc atcgcggttc ttaagcgtct cgctgatttg
     2281 ttgctcgaca ataaagctga ccgcttgctg aaagcggatg cggcaacgtt gctggcgctg
     2341 ctgaccagcg atgatgcgcc gaccgacgac gtgttaagcg cggagtttgt ggtgcgcaat
     2401 gaacacggcc tgcatgctcg tccaggtacc atgctggtca ataccattaa acaatttaac
     2461 agtgatatta ccgtgacaaa ccttgatggc accggcaaac cggcaaacgg acgtagtctg
     2521 atgaaagttg tggcacttgg cgttaagaaa ggtcatcgcc tacgctttac cgcccagggt
     2581 gcagatgctg aacaggcgct gaaagccatt ggcgacgcta tcgctgctgg tcttggggag
     2641 ggcgcataat gagcagacgt gttgctacga tcacccttaa tccggcttat gaccttgttg
     2701 gtttctgccc ggaaattgaa cgcggcgaag tgaacctggt gaaaaccacc ggtctgcatg
     2761 cggcgggtaa aggcatcaac gtggccaaag tattaaaaga cctgggaatt gatgtcaccg
     2821 ttggcggctt cctgggtaaa gacaatcagg atggttttca gcaactgttc agcgagctgg
     2881 gcattgccaa ccgtttccag gttgtacagg ggcgcacccg aattaacgtt aagctgacgg
     2941 aaaaagacgg cgaagtgacc gacttcaact tctcgggttt cgaagtcacc cccgccgact
     3001 gggaacgctt tgtgactgat tctctgagct ggctcggtca gttcgatatg gtctgtgtca
     3061 gcggaagctt accgtcaggc gtcagcccgg aagcgttcac cgactggatg actcgcctgc
     3121 gtagtcagtg tccttgcatt atctttgata gtagccgtga agcgttagta gcaggtttga
     3181 aagcggcacc gtggctggtg aaacctaacc gccgcgagtt ggaaatctgg gcaggccgta
     3241 aactgcctga aatgaaagat gtgattgaag ctgcacatgc gctacgtgaa caaggtattg
     3301 cgcatgttgt tatttcactg ggtgccgaag gcgcgctttg ggttaatgcc tccggcgaat
     3361 ggatcgctaa accaccgtca gtcgatgtcg taagcaccgt tggcgcaggg gattctatgg
     3421 ttggtggcct gatttatggc ttgctgatgc gtgaatccag tgaacacaca ctgcgtctgg
     3481 cgacagctgt tgcagccctg gcggtaagtc aaagcaatgt gggtattacc gatcgtccgc
     3541 agttggccgc aatgatggcg cgcgtcgact tacaaccttt taactgacag caggagaggc
     3601 ataatgaaaa cgctgctgat tattgacgct aatctcggtc aggcacgcgc ctatatggcg
     3661 aagaccctgc tgggcgcggc ggcgcgaaaa gcaaaactgg aaatcatcga caatccgaac
     3721 gacgcagaaa tggcgattgt tctcggtgat tccatcccga atgacagcgc gctgaacggt
     3781 aaaaatgtct ggctgggtga tatttcccgg gcagttgcgc accctgagct gttcctgagt
     3841 gaagccaaag gccatgcgaa accttacact gcgccggtca ctgcgacagc accggttgcc
     3901 gccagcggtc cgaaacgcgt agttgcggtg actgcttgcc cgactggcgt agcacacacc
     3961 tttatggcgg ctgaagccat tgaaaccgaa gcgaaaaaac gtggctggtg ggtgaaagtt
     4021 gaaacccgtg gttctgttgg cgcgggtaat gcaatcactc ccgaggaagt agccgcagcg
     4081 gatctggtga ttgtggcggc agatatcgaa gtggatctgg cgaaatttgc tggtaaaccg
     4141 atgtatcgta cctctaccgg tctggcgctg aagaaaacct cgcaggaact ggataaagcg
     4201 gttgctgaag caacgccgta tgaaccggcg ggcaaagctc aaacggcgac cactgaaggt
     4261 aagaaagaga gtgcaggcgc ttatcgtcac ttgctaacgg gcgtctctta catgctgccg
     4321 atggtcgttg ctggtggtct gtgtatcgcg ctttcttttg cttttggtat cgaagcgttt
     4381 aaagagccgg gtacgttggc agcggcgctg atgcagattg gtggtggttc agcctttgcg
     4441 ctgatggtgc cggtactggc aggttatatt gccttctcca ttgccgatcg tccgggcctc
     4501 acgccgggtc tgattggcgg tatgctggcg gtcagcaccg gttctggctt cattggcggt
     4561 attattgcgg gcttcctggc tggttacatt gcgaagttaa tcagtacgca actgaaactg
     4621 ccgcagagta tggaggcgct gaaaccgatc ctgatcattc cgctaatttc cagtctggtg
     4681 gtcggtctgg cgatgatcta cctgatcggt aaaccagttg ctggcattct cgaagggctg
     4741 actcactggc tgcagaccat ggggactgcg aatgcggttc tgctgggggc gatcctcggt
     4801 ggcatgatgt gtactgacat gggcggtccg gtaaacaaag cagcgtacgc attcggtgtg
     4861 ggtctgctga gtactcaaac ctatggcccg atggcggcga ttatggcggc aggtatggtg
     4921 ccaccgctgg caatgggcct ggcaacaatg gtggcgcgtc gcaaattcga caaagcgcag
     4981 caggaaggtg gcaaagccgc tctggtattg ggactgtgct tcatttcgga aggtgcaatt
     5041 ccgtttgctg ctcgtgatcc gatgcgtgtg ctgccgtgct gtatcgtggg tggggcgctg
     5101 actggcgcaa tctcaatggc gattggtgcg aaactgatgg caccgcacgg tggtctgttt
     5161 gttctgctga tccctggcgc tattacgccg gtattgggtt acctggtagc aattattgcc
     5221 ggtacgctgg tggcgggttt ggcctatgcc ttcctgaaac gtccggaagt ggacgcagta
     5281 gcgaaagcag cgtaataaaa ggtgtgttac agggcagaaa tttcctgccc tgtaacattt
     5341 gatgagcgca aagagaatga ccagcttgtg aatgtaattt tatgaattta aatggtttta
     5401 tctttcagta gaaacttcaa acgttcatca aacactaagg ttatttcctc aagggcagat
     5461 tattttctgc ccttttttag tgttcctgtt ctgtgcatcc cgtcacaaat tcctcgcttt
     5521 tgtaattcag gcatttaatc gcattttgtg agcctgctca aatctgccca ttttgcctct
     5581 ctggcggtaa tttactttac gtaaaacatg catactatga gcacatgttt aaatatgaac
     5641 acatgtttaa accgtctttg cagtatctat attcttgtgg attgacaggg ttggtaattt
     5701 tttaccagag gacgtgctat gcgcgaaaag gattatgtcg taattatagg ttcggcgaat
     5761 attgatgtcg ccggatattc acatgaatca ttaaattatg cggattcaaa tccaggtaaa
     5821 ataaaattta cgcctggtgg agtagggcgc aatattgcac aaaacctggc gttgctgggt
     5881 aacaaagcct ggctactgag cgccgtaggc agtgattttt atggtcaatc gctgctaacg
     5941 caaaccaatc aatctggtgt ttatgtcgat aaatgcctga ttgtgccggg agaaaatacg
     6001 tcgagttatt tatcattact cgataatacc ggtgaaatgc tggttgctat aaatgacatg
     6061 aatattagca acgctattac agctgaatat ctcgcacagc accgtgaatt tattcagagg
     6121 gcaaaggtca ttgtcgcgga ctgtaatatc agtgaagagg cactggcatg gattctggat
     6181 aatgccgcca acgtacccgt atttgtcgat ccggtttcag cgtggaaatg tgtcaaagtg
     6241 cgcgaccgtc taaatcagaa ccacactctg aagccaaacc gccttgaagc ggaaaccctg
     6301 agtgggattg cgctgtcagg gcgtgacgat gtggcaaaag ttgctgcctg gttccatcaa
     6361 catggcctga accgactggt attgagcatg ggcggcgacg gcgtttatta cagcgatatc
     6421 agcggtgaaa atggctggtc tgcgccgatc aaaaccaatg ttattaatgt taccggagcg
     6481 ggcgatgcca tgatggcggg acttgcttcg tgttgggtag acggaatgcc gtttgccgaa
     6541 tctgttcgtt tcgcacaggg atgttcgtca atggcgctct cctgtgaata caccaataac
     6601 cccgatttat cgattgccaa cgttatatcg ttagtggaga acgcagaatg tctgaattaa
     6661 aaatttcccc tgaattatta caaatttctc cggaagtgca ggacgcttta aaaaacaaaa
     6721 aaccggttgt ggcgctggaa tcgaccatta tttctcacgg gatgccgttc ccacaaaatg
     6781 cccagaccgc aattgaagta gaagaaacta ttcgtaaaca gggcgcagta cctgccacta
     6841 tcgccattat tggcggcgtg atgaaagtgg gtttaagcaa agaaaaaatt gaattactgg
     6901 gtcgtgaagg gcataacgtg accaaagtta gtcgtcgcga tttacctttt gttgttgccg
     6961 ccggaaaaaa tggcgcaacc actgtggctt caacgatgat tattgcggcg cttgccggaa
     7021 ttaaagtatt tgccaccggg ggaattggtg gtgtgcatcg cggggcggaa cataccttcg
     7081 atatttctgc cgatttgcaa gaactggcaa atactaatgt caccgttgtt tgtgccgggg
     7141 cgaaatctat tctcgattta ggattaacca ctgagtattt agaaaccttc ggtgtgccgt
     7201 taattggcta tcagactaaa gcgctgcctg cgtttttctg ccgtaccagc tcgtttgacg
     7261 tcagcattcg tctcgacagc gccagtgaaa ttgcccgtgc aatggcggtg aaatggcaaa
     7321 gcgggctgaa cggtggcctc gtggtagcga acccgatccc ggaacagttt gcgatgccgg
     7381 aggaatctat caatgcagcc atagatcaag ccgtcgccga agccgaagag cagggcgtta
     7441 ttggtaaaga aagtacaccg ttcctgctgg cgcgcgttgc tgagctgacc agcggtgaca
     7501 gcctgaaatc caatatccag ctggtgttca acaacgccat tctggcgagt gaaattgcca
     7561 aagaatacca gcgtctcgcg ggttaatttc tgacgggcag aaagccgtcg cccgtactca
     7621 tcaagcatac caaaacctgg cgctgacgcc gggtttactg gcatgaaggg aaaatcatta
     7681 tggatataat gagaagtgtt gtggggatgg tggtgttact ggcaatagca tttctgttgt
     7741 cagtgaataa aaagagcatc agtttgcgca cggttggagc cgcactgctg ctgcaaatcg
     7801 ctattggtgg catcatgctc tacttcccac cgggaaaatg ggcagtagaa caggcggcat
     7861 taggcgttca taaagtgatg tcttacagtg atgccggtag cgccttcatt tttggttcgc
     7921 tggttgggcc gaaaatggat gtcctgtttg acggtgcggg ttttatcttc gcctttcgcg
     7981 tacttccggc gattattttc gttactgcgc tcatcagtct gctgtactac attggcgtga
     8041 tggggctgct gattcgcatc cttggcagca ttttccagaa agccctcaac atcagcaaaa
     8101 tcgaatcttt tgttgcggtt accactattt tcctcgggca aaatgagatc ccggcgatcg
     8161 ttaaaccgtt tatcgatcgc gtgaatcgca acgagttgtt taccgcaatt tgtagcggga
     8221 tggcgtccat tgctggttcg gtgatgattg gttatgccgg aatgggcgta ccaattgact
     8281 acctgttagc ggcatcgctg atggcgatcc ccggcggtat tttgtttgca cgtattctta
     8341 gcccggcaac cgagccttcg caggtcacat ttgaaaatct gtcgttcagc gaaacgccgc
     8401 caaaaagctt tatcgaagcg gcggcgagcg gtgcgatgac cgggctgaaa atcgccgctg
     8461 gtgtagcgac ggtggtaatg gcgtttgtcg caattattgc gctgatcaac ggcattatcg
     8521 gcggaattgg cggctggttt ggtttcgcca atgcctctct ggaaagtatt tttggctatg
     8581 tgctggcacc gctggcgtgg atcatgggtg tggactggag tgatgccaac cttgctggta
     8641 gcctgattgg gcagaaactg gcgattaacg aattcgtcgc ttacctgagt ttctccccat
     8701 acctgcaaac gggcggcacg ctggaagtga aaaccgtggc gattatatcc tttgcgcttt
     8761 gtggttttgc taactttggt tctatcggtg ttgtcgttgg cgcattttcg gctatttcgc
     8821 caaaacgtgc tccagaaatc gcccagcttg gtttacgggc gctggcagca gcaacgcttt
     8881 ccaacctgat gagtgcgact attgccgggt tctttattgg tctggcgtaa tgctcgattt
     8941 gtagaccgga taaaacgtat taagcgttgc atccggcaat cgcgctattg caaataattt
     9001 ttactgcatc atcccggaga atttattctc cgggtccatc tccagcgcca gtcctgacaa
     9061 ctgctttctg tttttaatta gatatccttt cttttaatta ataaaccgtc gtgaatgaat
     9121 tgcgcgagaa catataaaag atgtcgataa gaaacgccta aatactctgc cgcttgcgta
     9181 tgcttttcgt gataaagatc accttcttgt gagagtaata taaatgctgc caggcgattc
     9241 actaacggaa atgattgatt ctgagttaaa gaaacaatat tacgataatt tttatgactt
     9301 aaggtgacgc aaagttttcg taaaaatagg gtgtcgttta ataacagcgg acggtaatgt
     9361 ttcataggga gcgcaaggca ccaacactct tcaatagcct gcaccgcacg cggttcatgg
     9421 tctttgtcga ttaactcaat ctcgccaata aaacagggag cggcaaagaa atcgatcagc
     9481 gacacgcgac cattggcaag cgtggcgtaa agcctggcgc ggcctcgcgt cagataaaac
     9541 agccatgacg gctgttgccc ttcctgcaca atgtagtcac gcgctaaaaa atgaaacagc
     9601 cgcgtatcgg ctgaaacatc cgttgaaaag caatccttaa acgcagattc actcatcaat
     9661 tgctgcttaa ggtcattatt gtggatttct ttcacgatgt ttatccgcta tgagatttct
     9721 catatttagc atacgtacgc tgttgttaag attcaatcat tggcccggtc gaattttctt
     9781 tgctgtagaa aaaaataacg caactggaaa cagaggaaat aaacaatgga aaagagaaaa
     9841 attattctgg attgtgagcc gggtcatgat gatgctattg caatgatgat ggcagcgaaa
     9901 catccggcaa tagatttatt aggcatcact attgtagcgg gtaatcagac gcttgataaa
     9961 acattaatta atggcctgaa tgtttgccag aaactggaga ttaatgttcc ggtttatgcg
    10021 gggatgccgc aacccattat gcgtaaacaa atcgttgccg ataatattca cggtgaaacc
    10081 ggactggatg gcccggtatt cgagccgctg acccgccagg cagaaagtac ccatgcggtg
    10141 aaatatatca tcgataccct gatggcaagc gatggcgata tcactctggt gccggttggt
    10201 ccgctttcaa atatcgcggt ggcaatgcgt atgcaacccg caatcctgcc caaaatccgt
    10261 gaaattgtgc taatgggcgg cgcttacggt acaggcaact tcaccccatc tgccgagttc
    10321 aatatctttg ccgacccgga agccgcccgc gtggtgttca cctccggcgt tccattagtg
    10381 atgatgggcc tcgatcttac caaccagacc gtttgcaccc cggatgtaat tgctcggatg
    10441 gaaagggtag gcggccctgc cggagagctg ttcagcgaca tcatgaactt cactctcaaa
    10501 acgcagttcg aatactacgg cctggctggc ggcccggtgc atgacgccac ctgcattggt
    10561 tatctgatca accctgatgg cattaaaacc caggatatgt acgtcgaagt ggacgtcaac
    10621 agcggccctt gctacgggcg tacggtctgc gacgaactgg gcgttcttgg caagcccgct
    10681 aataccaaag tcggcatcac tattgatacc gactggttct ggggcttagt cgaagagtgc
    10741 gtgcgcggct acatcaaaac ccattaagtg ttgataacaa gccgggacag tatcgcaaga
    10801 tgcaggcccg gctatttctg atgggcagaa agccgtcgcc caaaatgaca tgaagagata
    10861 atcactatgg atgtcatgag aagtgttctg ggaatggtgg tactgctggc aattgcgttt
    10921 ttactgtcag taaacaagaa gaagatcagc ctgcgtaccg ttggcgcggc gttagtgtta
    10981 caggtcgtga ttggcgggat tatgctttgg ttaccgccag ggcgttgggt cgcagaaaaa
    11041 gtcgcttttg gcgtccacaa agtgatggcg tacagcgacg caggtagcgc atttatcttc
    11101 ggttctctgg tcggaccgaa aatggatacc ttatttgatg gcgcaggatt tatctttggt
    11161 ttcagggtat taccggcaat tatcttcgtc actgcactgg tgagtattct ctactacatc
    11221 ggtgtgatgg ggatcttaat tcgcattctc ggcggtatct tccagaaagc tttaaatatc
    11281 agcaagatcg agtcattcgt cgcggtcacc accattttcc tcgggcaaaa cgaaattccg
    11341 gcgatcgtca aaccctttat cgatcgtctg aatcgcaatg aattatttac agcgatttgt
    11401 agtggcatgg cctcgattgc tggttcgaca atgattggtt acgccgcact gggcgtacct
    11461 gtggaatatt tgctggcggc atcgttaatg gcgatccctg gcgggatctt gtttgcccgc
    11521 ctgttaagcc cggctacgga atcttcgcag gtttctttta ataacctctc tttcaccgaa
    11581 acaccgccaa aaagcattat tgaagccgat gcgacagggg caatgaccgg gctgaaaatc
    11641 gccgccggtg tagcgacagt tgttatggca tttgtcgcca tcattgcgtt aattaacggt
    11701 attatcggcg gcgttggcgg ctggtttggt tttgaacatg cctcgctgga gtccatttta
    11761 ggttacctgc tggccccact tgcgtgggta atgggtgttg actggagtga tgcaaatctt
    11821 gccgggagtt tgattggaca gaaactggca ataaatgaat ttgtcgctta tctcaatttc
    11881 tcaccctatc tgcaaacggc tggcactctg gatgctaaaa ccgtggcgat tatttccttc
    11941 gcgttgtgcg gtttcgctaa ctttggttct atcggggtgg tggtgggggc gttttctgcg
    12001 gttgcgccac accgtgcgcc ggaaatcgcc cagcttggtt tacgcgcgct ggcggcggcg
    12061 acactttcta atctgatgag tgccaccatt gccgggttct ttattggttt agcgtagtgt
    12121 gcaatttatg agccggatta gatgcggtca agcgtcgaat ccggcaacat tgttacacca
    12181 ttggcactaa tgacagcgcg ttatcggcag acagggtagg gttgtttaag ctgccgctgg
    12241 cgcgtgaaat tgccgcgcag gccatcgcaa aacgggcgct gtcacggaaa ttgtttccct
    12301 ccagaaagct atataccaga cccgccataa aaccatcgtc agcaccaaaa ctgtctaccg
    12361 tcgtatgcgc tggcacagtc agcaaaaatt gttcaccatc cttttcgctg caataaactg
    12421 actcatcggg caaataaaca aacagttgct gaacaccttg ctgatgcaat gcattcactg
    12481 cggcattacg gtcagcatcg ctggtgatcg cctgtcccca taaaatttcc agctccggta
    12541 aagtgggttt cagggtgtga atatgcgcca gccagtgttt gattttgccc gctttgaatt
    12601 ctgaaacggt atcgacaaac accgggatct catcagcgag ggtaaagacc cattccagcg
    12661 cctcggctgt caggttgcaa tcagccagta ccacgcccgc atgacgaagt aaatcgcgcg
    12721 acccgttcag tagttgcggt gtcaactgtt ccagcagatg ggtatcgtta attgccagca
    12781 cggtttgatc gtctcgattg gcaattgcca gatacgtcga tgtgctttga ccatgcaaac
    12841 gaacgcagcc ggagacattc acgccggcac ggcgcgtttc ttccaggagc atttcgccat
    12901 aaaagtcatc gccaatcact gaaagcaaat ggacgtcacg gcctaacagc gccagattgt
    12961 gggcgatatt gcgtccaacg ccgcctgccg aacaatgaat tgtaccggga tgagaagccg
    13021 cttgcgggta atggatatcc gccatcccgc gaatatccat attgattgtc cccaccacta
    13081 cgcagtattc ctgctcggtg agaatgtaac ctttgtcttt aatccggcct ttgcgcatta
    13141 aatccataat atgcgccgca acgcgcgaac gactgatttg cagcatgtcc gcaatttcgt
    13201 tctgctgaat cagcgggtta cgccgtaaaa ttgcaaggat ctccttttcc cggttattca
    13261 tcttcaggct accgcttttt cagtttgttg cgctttcagc caggcgatct cttctgccca
    13321 gatatccggg ttgatggttt caaggatcag cggaatgccg tcgaaacggt cgttctgcat
    13381 aatccagcgg aacgcatcat gaccgatatt gccttcaccg aggctatgat ggcggtcaac
    13441 gcggctgcca aaggtacttt tcgcatcgtt aaggtgcatc ccgcgcaaat acttaaagcc
    13501 gacaatacgg gcaaaatcag cgaacgtttt ttcgcattcg gctggagtac gcaaatcata
    13561 cccggcagcg aaagcatggc aggtatcaat gcagacgccg acgcgggatt tatcttccac
    13621 gccgtcgata atcgccgcga gatgttcgaa tttaaaccct aagttactgc cctgaccggc
    13681 ggtgttttca atcaccgctg ttacaccttg agttttatcc agcgcaatgt tgatggattc
    13741 ggcaatacgc gcaaggcaat cctcttctga aatctgcatc agatggctgc cagggtggaa
    13801 gttgagcaaa gaaagcccca gttgttcgca acgctgcatt tcatctataa aggcatcgcg
    13861 cgatttttcc agagcttcag tgaccggatg tccgaggttg atcagataac tgtcgtgggg
    13921 aagaatttgc gccgatgtgt agtgatattt ttcacaggcg gctttgaatt catcgatggt
    13981 ttgcgtcgtg agcggtgcgg cacgccactg acgttggttt ttggtgaaca aggcaaacgc
    14041 ggttgcgtcg atttcggcgg cgcgaattgc ggcatttgcc agaccgccag cagcactaac
    14101 gtgcgctcca atgtatttca tgcgaggact cctgttaaac ccgctggagg aaaacggtaa
    14161 tgatagcggg ttaacaggag caagaagtag tggtttatgc gatgacgctt tgaatcacat
    14221 agttaatcgc accaccacca acaatcagcc aggcaaacag taccagtgcc atcagcagag
    14281 gtttcgcccc agcttttttc agcgcgctga cgtgagtggt cagacccagc gccgccatcg
    14341 ccattgccag caggaaggta tccagcgtta ccagcatgtt caccacgctc tgcggtaaca
    14401 ggtggaacga gttaaagatg gcaactacga tgaacaagat ggcaaaccac ggaatagtga
    14461 ttttgctttt ctcgccgctg ttcgccccag acagctgttt aacacgcgcc gccagcagga
    14521 tgaggaacgg agccagcatc atcacgcgca gcattttgga aataactgct gcgttttctg
    14581 catccgggct gatggcatga cctgccgcca ccacctgcgc cacttcgtgc acagtagaac
    14641 caatgtagat accgaaagtt tccggactaa accattgaga catcagcgga tatatcgccg
    14701 ggtagaggaa aatcgcgacg gtcccgaaga taacaacggt tgcaacagcc acggttactt
    14761 tactggcttc cgctttcact accggctcag tcgccagtac cgcggcagca ccacagatac
    14821 tgctaccggc accgatcaac caactggtgt gcttatccag accaaacact ttctgcccca
    14881 ggaagcaagc cagcaggaag gtactggaca gcgtcagcac gtcaatgatg atcccactga
    14941 taccgacatc ggcaatttgc gagaacgtca gacggaagcc ataaagaatg atacccagac
    15001 gtaataaata ttgcttggca aacagcacac caccgtcaca gcttttccag atgtgcggat
    15061 agatggtgtt gcctaaaacc atccccaaca agattgcgag ggtgagggca ctaaacccgg
    15121 cacccgcaac cgcgggaatg gaaccacccc acagggcgac cccggtgata actgcactca
    15181 gggctaaccc cggaataaaa tgccacagtg tacgatgttg tttctgtaag gtgatattcg
    15241 tcataaccct ctcctttaac cgggcataag gttacgacta actggtttaa aaataaaatt
    15301 gattatatat ttataattaa tctttataag tggtaagcga ctatgcacat caccctccgg
    15361 cagttggaag tttttgcaga agtattgaaa agtggatcaa ccacccaggc gtcggtgatg
    15421 ctggcgttgt cgcaatcagc agtgagcgca gccttgactg acctggaagg gcagcttggc
    15481 gttcaactgt ttgatcgcgt ggggaaaaga ctggttgtta atgaacacgg gcggctgctc
    15541 tatccgcgag cgttggcact gcttgaacag gcggttgaaa tcgaacaact gtttcgcgaa
    15601 gacaacggcg cgattcgtat ctatgccagt agtaccatcg gtaactacat tctgcctgca
    15661 gttatcgccc gttatcgcca tgattatccg cagttgccga ttgaacttag cgttgggaac
    15721 agccaggacg tgatgcaagc ggtgctggat ttccgcgttg atattggctt tattgaagga
    15781 ccgtgccaca gcactgaaat catttctgaa ccgtggctgg aagacgagct ggtggttttc
    15841 gccgcgccga cttcgccgtt ggctcgtggt ccggtcacct tagaacagct ggccgctgcg
    15901 ccgtggatcc tgcgtgaacg cggttccggc acgcgggata ttgtcgatta tctgttgctg
    15961 tcacatttac cgaagtttga gatggcgatg gaattaggta actccgaggc aatcaaacat
    16021 gcggtgcgtc atgggttggg aattagttgc ctgtcgcgac gtgtgattga agatcaattg
    16081 caggcaggca cattaagtga agttgcggtc cctctgccgc gcctgatgcg tacgttgtgg
    16141 cgtatacatc atcggcaaaa acacctttcc aacgcgctac ggcgctttct ggactattgc
    16201 gatcccgcaa atgtgccgcg ttaagttgct gtacaagaac atgctggtgc tgtgtcgatt
    16261 tcgtgacgca gcgccttcag catgcattcg ccagaaaaga gattggctgc tttacttata
    16321 atccctgggc gatcatgaag gtgtcttata accgtgtatt tctgccggaa ggattgccaa
    16381 tcgtctgcta caatcgcgcc atcattttta agatggatag catttttgta tgggttccga
    16441 aactaaaact acagaagcgc cgggcttacg ccgtgaatta aaggcgcgtc acctgacgat
    16501 gattgccatt ggcggttcca tcggtacagg tctttttgtt gcctctggcg caacgatttc
    16561 tcaggcaggt ccgggcgggg cattgctctc gtatatgctg attggcctga tggtttactt
    16621 cctgatgacc agtctcggtg aattggctgc atatatgccg gtttccggtt cgtttgccac
    16681 ttacggtcag aactatgttg aagaaggctt tggcttcgcg ctgggctgga actactggta
    16741 caactgggcg gtgactattg ccgttgacct ggttgcagca cagctggtca tgagctggtg
    16801 gttcccggat acaccgggct ggatctggag tgcgttgttc ctcggcgtta tcttcctgtt
    16861 gaactacatc tctgttcgcg gctttggtga agcggaatac tggttctcac tgatcaaagt
    16921 cacgacagtt attgtcttta tcatcgttgg tgtgctgatg attatcggta tcttcaaagg
    16981 cgcgcagcct gcgggctgga gcaactggac aatcggcgaa gcgccgtttg ctggtggttt
    17041 tgcggcgatg atcggcgtag ctatgattgt cggcttctct ttccagggaa ccgagctgat
    17101 cggtattgct gcaggcgagt ccgaagatcc ggcgaaaaac attccacgcg cggtacgtca
    17161 ggtattctgg cgaatcctgt tgttctatgt gttcgcgatc ctgattatca gcctgattat
    17221 tccgtatacc gatccgagcc tgctgcgtaa cgatgttaaa gacatcagcg tcagtccgtt
    17281 caccctggtg ttccagcacg cgggtctgct ctctgcggcg gcggtgatga acgcagttat
    17341 tctgacggcg gtgctgtcag caggtaactc cggtatgtat gcgtctactc gtatgctgta
    17401 caccctggcg tgtgacggta aagcgccgcg cattttcgct aaattgtcgc gtggtggcgt
    17461 gccgcgtaat gcgctgtatg cgacgacggt gattgccggt ttgtgcttcc tgacctccat
    17521 gtttggcaac cagacggtat acctgtggct gctgaacacc tccgggatga cgggttttat
    17581 cgcctggctg gggattgcca ttagccacta tcgcttccgt cgcggttacg tattgcaggg
    17641 acacgacatt aacgatctgc cgtaccgttc aggtttcttc ccactggggc cgatcttcgc
    17701 attcattctg tgtctgatta tcactttggg ccagaactac gaagcgttcc tgaaagatac
    17761 cattgactgg ggcggcgtag cggcaacgta tattggtatc ccgctgtttc tgattatttg
    17821 gttcggctac aagctgatta aaggaactca cttcgtacgc tacagcgaaa tgaagttccc
    17881 gcagaacgat aagaaataag tttcctccct tccttgctaa gccctctcaa ccgagagggc
    17941 tttttcaatt ccatttccct gacaaatcat gcggatataa aatttaacat ttggattgat
    18001 aattgttatc gtttgcatta tcgttacgac gcaatcaaaa aaggctgaca aatcagaggc
    18061 tgttccggct ttctgggatg atcacctgca tacaaataag tccaccgcga tgctgccgta
    18121 cgcaagggga cgtaaagaag atgtgagcga tacccatttt attttcgtag ttacctcatg
    18181 gagatatgga atgtttaggt tgaacccttt cgtacgggtc gggctgtgtt tgtccgctat
    18241 ttcttgtgca tggcctgtgt tagcggtcga tgatgatggc gaaacgatgg ttgtcactgc
    18301 atcttccgtg gaacaaaatc ttaaagatgc acccgccagt atcagcgtca ttacccagga
    18361 agacctgcag cgaaaaccgg tacagaatct gaaggatgtc ctcaaagaag tgcctggcgt
    18421 acaactgacg aacgaagggg ataaccgtaa gggcgttagt attcgtggtc tggacagcag
    18481 ctacaccctg attcttgtcg acggtaaacg cgttaactcc cgcaatgccg tcttccgcca
    18541 caatgatttc gatctgaact ggatcccggt cgattccatc gaacgtattg aagtggttcg
    18601 tggcccgatg tcgtcgctat acggctccga tgcgctcggc ggtgtagtga atatcatcac
    18661 caaaaaaatc ggtcagaaat ggtcgggtac cgttaccgtc gataccacca ttcaggaaca
    18721 tcgcgatcgc ggtgatacct ataacggtca gttctttacc agtggaccat taattgatgg
    18781 tgtgctggga atgaaagctt acggcagcct ggcaaaacgt gaaaaggatg acccgcaaaa
    18841 ctcaacgacc accgataccg gagaaacgcc gcgtattgaa ggattctcca gccgcgacgg
    18901 caatgtcgaa tttgcctaga caccgaatca aaatcacgat tttactgccg gatacggttt
    18961 cgaccgtcag gatcgtgatt ccgattcgct ggacaaaaac cgcctggaac gccagaacta
    19021 ctgcgtcagc cataatgggc gttgggatta cggcaccagc gaactgaaat cctacggtga
    19081 gaaagtcgag aacaaaaacc ctggcaacag cagcccgata acttccgaaa gcaatacggt
    19141 cgacggcaaa tacacgttgc cgctgacggc gattaatcag tttctcacgg ttggcggtga
    19201 atggcgtcac gacaaactta gcgatgcggt gaacctgacc gggggaacca gctccaaaac
    19261 gtctgccagc cagtacgcgc tgtttgtgga agatgaatgg cggatcttcg agccgctggc
    19321 gctgacgacc ggcgtgcgta tggacgatca cgaaacctac ggtgaacact ggagtccgcg
    19381 tgcctacatg gtttataacg ccaccgacac cgtaacggtg aaagggggct gggcgacggc
    19441 atttaaagca ccttctctgt tgcaacttag ccctgactgg acgagcaatt cctgccgtgg
    19501 cgcatgtaag attgtgggta gcccggatct gaaaccagaa accagcgaaa gttgggagct
    19561 ggggctttac tacatgggtg aagaaggctg gctggaaggg ggtgaatcca gcgttatcgt
    19621 tttccgtaac gatgtgaaag atcgtatcag catcagccgt acgtctgacg tcaacgctgc
    19681 accgggctac caaaactttg ttggttttga gacgggcgct aacggacggc gcataccggt
    19741 atttagctac tacagcgtta acaaagctcg tattcagggc gtggaaaccg aactgaaaat
    19801 tccgttcaac gatgaatgga aactgtcgat caactacacc tacaacgatg gtcgtgatgt
    19861 cagcaacggc gaaaacaaac cgctatccga tctgccgttc catactgcta acggtacgct
    19921 ggactggaaa ccgctggcgc tggaagactg gtcattctat gtttctggtc actataccgg
    19981 gcagaaacgc gccgacagcg cgacggctaa aacaccgggc ggttatacca tctggaatac
    20041 cggcgcggcc tggcaggtga ctaaagacgt caaactgcgc gcaggcgtgc tgaaccttgg
    20101 cgacaaggat ctcagtcgtg acgactacag ctataacgaa gacggacgtc gttactttat
    20161 ggcagtggat tatcgcttct gatgagaaga tgcccggcga accgggcgga ctttcacttc
    20221 agtaaatact gcgcatggaa gcgcaggtga tcctctataa aagaggcgat gaagtagtaa
    20281 ctgtgatcat atcccggctg aatacgcagc gtcatcggcc acgctttctg gcgcgcggct
    20341 tctgccaata ccgcaggttg caactggtcg gcaagaaact gatcattatc gccctgatcg
    20401 ataagcgtcg ggatcgcatc ctgcgcgtta ctggcataca tcagcgcgca actatcccat
    20461 tccagcatgc atttttgtcc tcacctaaat aggtgctaaa cgctttgatt ccccacggga
    20521 cgctgcacgg attcacaatg ggcgcaaagg ccgaaacgct ggtgtattta cccggatttt
    20581 tcagcgccat aatcagcgca ccgtgaccac ccattgagtg accgctaatg gcgcagcggt
    20641 cgctgacatt aaattgcgac tgaaccagcg ccggtaattc atcgcgcaga taatcataca
    20701 tccggtaatg cgtcgcccac ggcggttgcg tggcattaag ataaaagcct gcgccctggc
    20761 ctaaatcgta gccatcgtcg ttggcaacct tttcgccgcg cgggctggtg tctggcatca
    20821 ccagtacaat ccccagttcc gccgctaccc gctgggcacc cgccttggtg gtgaagttct
    20881 cgtcattgca ggttaatccg gaaagccagt acagcactgg tggcggggtg tgatcacgag
    20941 ggggggggag aaagatactg aacgtcatcg ggcagtttaa ggtactggag tcgtgtcgcc
    21001 agcgttgctg ccagccttca aaacagcggt gctcttcgag catttccatg cgtggctcct
    21061 tgttgtgttg tttgcaattt cttcatcata ccgtttattc aatgtgctgt gagtaacttt
    21121 cacttccgta tttgcataac gatgttttaa catctgctga tgaaaggcag cggcaattac
    21181 aataattatc gctgtgaata ctggattatg tgcgccgcct cacgcacaat aatcaagctg
    21241 taaatcagct taataacttt gcccccacgc agggtggagg cgtcacacct gcaggagaaa
    21301 tcataaatgc catcactcag taaagaagcg gccctggttc atgaagcgtt agttgcgaga
    21361 ggactggaaa caccgctgcg cccgcccgtg catgaaatgg ataacgaaac gcgcaaaagc
    21421 cttattgctg gtcatatgac cgaaatcatg cagctgctga atctcgacct ggctgatgac
    21481 agtttgatgg aaacgccgca tcgcatcgct aaaatgtatg tcgatgaaat tttctccggt
    21541 ctggattacg ccaatttccc gaaaatcacc ctcattgaaa acaaaatgaa ggtcgatgaa
    21601 atggtcaccg tgcgcgatat cactctgacc agtacctgtg aacaccattt tgttaccatc
    21661 gatggcaaag cgacggtggc ttatatcccg aaagattcgg tgatcggtct gtcaaaaatt
    21721 aaccgtatcg tgcagttctt tgcccagcgt ccgcaggtgc aggaacgtct gacgcagcaa
    21781 attcttattg cgctacaaac gctgctgggc accaataacg tggctgtctc gatcgacgcg
    21841 gtgcattact gcgtgaaggc gcgtggcatc cgcgatgcaa ccagtgccac gacaacgacc
    21901 tctcttggtg gattgttcaa atccagtcag aatacgcgcc acgagtttct gcgcgctgtg
    21961 cgccatcaca actgattaaa aggcaggaac catggagcgc aacgtcacgc tcgattttgt
    22021 tcgcggcgtc gccattctgg ggatcctgct attaaacatc agcgcctttg ggctaccaaa
    22081 ggcggcttat ctcaatcccg cctggtacga tgctattacg ccgcaggatg catggacctg
    22141 ggcatttctc gatctcatcg gccaggtgaa attcctcacg ctttttgcgc tgctgtttgg
    22201 tgctggcctg caaatgttgc tgccccgtgg cagacgctgg atccagtcgc ggttaacgct
    22261 gttagtcttg ttgggcttta ttcacggttt attgttctgg gacggcgata ttctgctggc
    22321 ttacgggctg gtggggcttg gcgttttgct gttattgggg ctgatttccg acagccagac
    22381 cagccgcgcc tggacgccgg atgcatcggc tattttgtat gaaaaatact ggaagcttca
    22441 cggcggcgtt gaagcgatca gtaatcgtgc cgatggtgtt ggcaacagtt tactggcact
    22501 gggcgcacag tatggctggc aactggcagg gatgatgctc attggtgcgg cgctgatgcg
    22561 cagcggctgg ctgaaagggc agttcagctt acgtcactat cgtcgtactg gttttgtgct
    22621 ggtggcgatt ggggtgacca ttaaccttcc tgccatcgcc ctgcaatggc agctggactg
    22681 ggcgtatcgc tggtgtgcct tcttacttca aatgccgcgg gaactgagtg cgccgtttca
    22741 ggcgattggc tatgcgtcgc tgttttatgg cttctggccg caattgagcc gctttaagct
    22801 ggtgcttgcg atcgcctgcg tcggacggat ggcgctgacc aactatctat tgcaaacgct
    22861 gatttgtacc acgctttttt accacctcgg tttgtttatg cattttgacc gcctggagct
    22921 gctggcgttt gttattccgg tatggctggc gaatatcctc ttctctgtta tctggctgcg
    22981 ttacttccgc caggggccgg tggaatggct ctggcgtcag ttaactttgc gtgctgccgg
    23041 accggcaata tctaaaacat caagataacg atctggatta cattcgttaa caaaacggct
    23101 gtaaccgttt ccattgctgt gactcgattc acgaagtcct gtattcagtg ctgacaaaat
    23161 agccgccagc aagcagtcat ttactgcaat ctcataacag gtagtgaata tgatcaccat
    23221 tcgtgatgta gcgcgtcagg ctggcgtctc tgtggcaacg gtttcccggg tgctcaataa
    23281 cagcacgctg gtcagtgccg acacgcgtga agcagtaatg aaagccgtga gtgagctgga
    23341 ttatcggcca aacgccaatg cccaggcgct ggcaactcag gttagcgaca ccattggcgt
    23401 ggtggtgatg gacgtttctg atgcgttttt cggcgcgctg gtaaaagcgg tggatctggt
    23461 cgcccagcag catcagaaat acgtgctaat cggcaatagc tatcatgaag cggaaaaaga
    23521 gcgtcacgcc attgaggtgt taattcgcca gcgttgtaat gcgttgattg ttcactcaaa
    23581 agcattgagt gacgatgaac tggcgcaatt tatggataac attcccggta tggtgttaat
    23641 caaccgcgtt gtgccggggt acgcccatcg ttgcgtttgc ctggataatc tcagcggtgc
    23701 ccgaatggcg acgcgcatgt tgctgaataa cggtcatcaa cgtattggtt atctttcttc
    23761 cagccacggc attgaagatg acgccatgcg taaagcaggc tggatgagtg cgttgaaaga
    23821 gcaggatatt attccgccgg aaagctggat tggcactggt acgccggaca tgccgggcgg
    23881 tgaggcggcg atggttgaac tgctggggcg caatctacaa cttaccgctg tatttgctta
    23941 taacgacaat atggccgctg gcgcactgac agcattaaaa gataatggca ttgcgattcc
    24001 gttacatctc tcaatcatcg gtttcgatga tattcccatc gcccgttaca ccgacccgca
    24061 attaacgacc gtgcgttatc ccattgcttc aatggctaaa ttagccaccg aactggcctt
    24121 gcagggggca gcaggcaata ttgatcctcg tgccagccac tgttttatgc cgacgttagt
    24181 gcgtcgccat tctgtcgcaa cgcgccagaa tgcggcggcg atcactaact caacaaatca
    24241 ggcgatgtaa ccgctttcaa tctgtgagtg atttcacagt atcttaacaa tgtgatagct
    24301 atgattgcac cgttttaatg ttgtaacccg tatgtaacag tgaataatca ctttcaccga
    24361 ggtaatagcg tcataacaac aattaaagcc gttttctgga gcgttaccgg gcatggaaga
    24421 acgaatttta aaaagtgagc ttcggcgttc agtaacactt cattaactct actgccccgc
    24481 cgagcattta tctcaagcac taccctgcat aagaaaaacc ggagatacca tgaataagaa
    24541 ggtgttaacc ctgtctgctg tgatggccag catgttattc ggtgccgctg cacacgctgc
    24601 tgatactcgc attggtgtaa caatctataa gtacgacgat aactttatgt ctgtagtgcg
    24661 caaggctatt gagcaagatg cgaaagccgc gccagatgtt cagctgctga tgaatgattc
    24721 tcagaatgac cagtccaagc agaacgatca gatcgacgta ttgctggcga aaggggtgaa
    24781 ggcactggca atcaacctgg ttgacccggc agctgcgggt acggtgattg agaaagcgcg
    24841 tgggcaaaac gtgccggtgg ttttcttcaa caaagaaccg tctcgtaagg cgctggatag
    24901 ctacgacaaa gcctactacg ttggcactga ctccaaagag tccggcatta ttcagggcga
    24961 tttgattgct aaacactggg cggcgaatca gggttgggat ctgaacaaag acggtcagat
    25021 tcagttcgta ctgctgaaag gtgaaccggg ccatccggat gcagaagcac gtaccactta
    25081 cgtgattaaa gaattgaacg ataaaggcat caaaactgaa cagttacagt tagataccgc
    25141 aatgtgggac accgctcagg cgaaagataa gatggacgcc tggctgtctg gcccgaacgc
    25201 caacaaaatc gaagtggtta tcgccaacaa cgatgcgatg gcaatgggcg cggtagaagc
    25261 actgaaagca cacaacaagt ccagcattcc ggtgtttggc gtcgatgcgc tgccagaagc
    25321 gctggcgctg gtgaaatccg gtgcactggc gggcacagta ctgaacgatg ctaacaacca
    25381 ggcgaaagcg acctttgatc tggcgaaaaa cctggccgat ggcaaaggtg cggctgatgg
    25441 caccaactgg aaaatcgaca acaaagtggt ccgcgtacct tatgttggcg tagataaaga
    25501 caacttggct gaattcagca agaaataaga ctgattgggc gcagtcgttt actgcgccct
    25561 tattgtatgg ccaacaaggt aaaattatat ggtcagctca acgactccgt cttccgggga
    25621 atacttgttg gaaatgagcg gtatcaacaa gtcctttcct ggtgttaagg cacttgataa
    25681 cgttaattta aaagtccggc cacattctat ccatgcatta atgggggaaa acggtgcagg
    25741 aaaatcgaca ttattaaaat gcctgtttgg tatttatcaa aaagactccg gcaccatttt
    25801 attccagggt aaagagatcg atttccattc cgccaaagag gcactggaaa atggtatttc
    25861 gatggtacac caggagttaa acctggtatt acaacgttcg gtgatggata acatgtggct
    25921 ggggcgatat cccaccaaag gcatgtttgt cgatcaggac aaaatgtacc gcgaaaccaa
    25981 agcgattttt gatgaactgg atattgatat cgatccgcgt gcgcgcgtcg gcacattatc
    26041 cgtttcgcaa atgcagatga tcgaaatcgc caaagcgttt tcctataacg cgaaaattgt
    26101 gattatggat gaaccgactt cttcgttaac cgaaaaagag gtcaatcatc tgttcactat
    26161 tattcgtaaa ttaaaagagc gcggctgcgg tattgtttat atctcgcata aaatggaaga
    26221 aatcttccag ttatgtgatg aagttaccgt attgcgcgac ggtcagtgga tcgccaccga
    26281 accgctggca ggactgacga tggacaagat catcgccatg atggttgggc gttctcttaa
    26341 tcagcgtttt cctgacaaag aaaacaagcc gggcgaagtc atcctcgagg tgcgtaacct
    26401 aacgtcactg cgccagccgt cgattcgcga tgtctcgttt gatctgcata aaggggagat
    26461 cctcggtatt gccgggctgg tgggggcgaa acgtaccgat attgttgaga cgttatttgg
    26521 tattcgcgag aaatcggctg gcaccattac gttgcacggc aaacagatca ataaccataa
    26581 tgccaacgaa gccataaacc acggatttgc actggtaact gaggagcgcc gctcaacggg
    26641 aatttatgcc tatctggata ttggttttaa ctcgttaatt tccaatattc gcaactacaa
    26701 aaataaagtt ggtttactgg ataactcgcg gatgaaaagc gatacccagt gggtgattga
    26761 ttcgatgcgg gtaaaaacgc cgggtcatcg gacgcaaatt ggttcgctct ccggtggtaa
    26821 tcagcaaaag gtgattattg gtcgctggct actaacgcaa ccagaaatat taatgctcga
    26881 tgaaccgacg cgcggtattg atgtcggggc gaagtttgaa atttatcagt taattgccga
    26941 actggcgaag aaaggcaagg ggattattat tatctcctct gaaatgcctg agttgttagg
    27001 gataacagac cgtattctgg tcatgagcaa tggtctcgtt tccggaattg tcgatacaaa
    27061 aacaacaacg caaaacgaaa ttctgcgtct tgcgtctttg cacctttaag attaggggct
    27121 tcccatgagt gcgttaaata agaaaagttt tcttacttac ctgaaagagg gcggtattta
    27181 cgtcgttctt ttagttttgc tggcgattat tattttccag gacccaacat ttttaagtct
    27241 gttgaactta agtaatattc tcacccagtc atcggtgcgt attattatcg cgctcggtgt
    27301 ggcagggtta attgtcaccc aggggaccga tctttctgct ggtcgtcagg tagggctggc
    27361 ggcagtggtg gctgcgacat tattgcagtc catggataac gccaacaaag tgttcccgga
    27421 aatggcgacg atgccgattg cgctggttat tctgattgtc tgtgccattg gtgcggtgat
    27481 cggtttgatc aacggtctga ttatcgctta tctcaacgtg acgccgttca ttaccacgct
    27541 cggcacgatg atcatcgtct atggcatcaa ctcgctctat tacgactttg tcggggcgtc
    27601 gccaatttct ggttttgaca gtggcttctc tacctttgct cagggctttg tcgcgctggg
    27661 gagtttccgt ctctcttaca tcaccttcta cgcgttgatt gcggtggcgt tcgtctgggt
    27721 gttgtggaac aaaacccgct tcggtaagaa catttttgcc attggcggta acccggaagc
    27781 ggcaaaaata tctggtgtca acgtcggcct gaacctgctg atgatctacg cgttgtctgg
    27841 cgtgttctat gcctttggcg ggatgttaga agccggacgt atcggctctg ccaccaacaa
    27901 cctcggcttt atgtatgagc tggatgctat cgcggcgtgc gtggtaggcg gtgtatcgtt
    27961 cagcggcggt gtggggacgg tgattggcgt ggtgaccggg gtaattattt ttaccgtcat
    28021 caactatggc ctgacgtata tcggcgtaaa cccatactgg cagtacatca tcaaaggggc
    28081 gattattatc ttcgccgtag cgctggattc actgaaatac gcgcgtaaga agtgatgtga
    28141 ttgcggatat tgccggatgc gtctctggtt ttacaacgtt accgggtgtt ctttctcgcc
    28201 tttcttaaac ttcacttccc cgagctcaat acaacccacc gggcagacgt gaccacaaag
    28261 cagacaaccc acacatttct cggtattaca atgcggtgtg cgggttttct cgctccattc
    28321 catcgcctgg tgaccgccgt cgtaacagga aatataacag cgtccacagc caacacattt
    28381 gtcaagattg atacggggat agacaatata actgcggtct aaatcttccg ccgggacaat
    28441 attgttattc gccagaccta ccatctcctg caacgaatca aatccctgat cggcgagata
    28501 atgcgataac ccgctcgcca tatcttctac gatgcgatag ccgtactgca taatgccggt
    28561 ggtgacctgt aacgttgctg cgccgagcaa taaaaactcc gccgcatctt cccaggtttc
    28621 aatgccgccg ataccgctga ttgggaaatc gcgcagttct ggatgggtgc gcatttgctg
    28681 aatgaagcgc agggcgatcg gttttaccgc tttaccggaa tatccggaaa tactcgattt
    28741 accgttaacg atcggcatac cgattttctg attaagatcg atattggtga tggatttaac
    28801 ggtgttaatt gccgcaatgc catctgcgcc gccgcgcttc gccgccagcg ccacttcgca
    28861 catatcaccg atattcggcg tcatcttcgc cagcattggc agcgtggaac cccgtttcac
    28921 tgcccgacaa tatttttcta ccagctccgg gctttgcccg acatcgctac ccatcgcatg
    28981 agaagtcatt tgcggacagg agaagttaca ctcgatcata tccgcgccag ctgctcccat
    29041 tgctgctcat tttcccccat gatcgaagcg atcaatactt tgtccgggta atcttccttc
    29101 agccgacgca gggcggtcag attctcttcc aacggatgtt cagcaatctg ctccatattt
    29161 ttgaagccga taaaaccggt atcttctttc accagatgat caaaacgcgg cgagacttcg
    29221 ttggcgataa aaaagccgat cgttttaaac accacaccgc cccaacctgt gtcgtaggct
    29281 ttggcacaca tctcatagca gttgcctacc ggcgaagaag agaggcagaa cgggttggga
    29341 aagttcacgc cgcaaaaagt aatcgaaaga tctttcgtta acatgagcaa gctccctcta
    29401 aatagtgatg aatcgcctcg gcggcttctt tcccggtttt cacggcatag accacggttt
    29461 tgtcaccctc aacaatatcg ccagcagcaa agacttgcgg atcgcggatc tgataatttt
    29521 gtgttttgat ggtgttacgc tgctgctcta actccgcaaa ggcatccagt ctggcatgct
    29581 gaccgacggc gagaataatt ttatctgccg ccatcgtcag ttcgcctgat aaccgtacat
    29641 gcttaaacgt gactttattg ccttccacgg ctactggcgt gaatccatcg atgatcgaaa
    29701 cgcccagctc ccgggcgctg gcaaactctt tttcgcttgc cggaaactca tctaactctt
    29761 cacgtgctac gcaagttacc gcctgacagc cgagaacttt cagcgtgctg gctacgtcca
    29821 tcgcgacatc accgccgccg ataattaatg cgctttgtgg aatgctgata tcgccttgcg
    29881 cctgtcgtac acgttgcagg aagtcgacgg caatctcaac gtcgctatgc tcaaacagcg
    29941 gtagcccgga accgcttgat aaccccacgg tgaccagtac cgcgcggttt tccgctttca
    30001 gttgctccag ggtgagtgtt ttaccgattt cgttattgca cttaatggtc acgcccattt
    30061 tttcaatacg ggcgatctct gcatccagca ctgattgcgg taaccggaat tgcggaatac
    30121 cgttacgcaa ccagccaccg gggtgcgctt ctttctcata aatcgtgacg tcataaccct
    30181 ggtttgtcag tgtcacactg gcctgcaatc cggcaggacc tgcgccaata atcgcgactt
    30241 tgccgagcgt tttagtaccg ggctgataaa tttccatccc ggtttgttgt tcaaaatcag
    30301 taacaaaacg ctgtaagcgg ccgatatcaa tgggagcatc aacaccggca cgggtacaac
    30361 cgctttggca taatttctcc gtcgggcaca ctctggcaca aacggcaccg agggcattat
    30421 tttcgcgaat tgtctcggca gcgcctttaa aattacgaaa gtagattgag cgaataaatt
    30481 tccccggatc ggtctgtgcc gggcaagcct gactacaggg agcgtcgtga cataataaac
    30541 agcgagaggc ttctttaatc gccagtaacg gcgtaaaagc cggagtgagt tcatccagat
    30601 aattttgttg cggcatgtga tcttccttaa tgctatgagt aattatggcg aacgctaaat
    30661 aaatattgcg tgctgatatt aaggctatgc gcgtggggaa atttaagctg tgaaaggatt
    30721 cacaaatagc tgcggttcag aatgttatat ccgcagctta ttgagtgaag taataattat
    30781 tctcaataat taacagaaaa gagaaaatat taacgataga aaataagata aacaatcatg
    30841 ccgatagata aaattccgaa gccaatatat gccaccatat aagcgaagaa gagtccggga
    30901 ttttttgctc gccagacata caccggttcc ggtgcatttt taacccgttt atcaatcgcg
    30961 cctgcgcgcc agcctttcca tgcttcgcgg aaacaaaata tcgggataag gaaaaaaacg
    31021 gcaacgacaa aaaactgctg tacatccata actactttcc ttgtttcttt tgtaattcaa
    31081 gtaactgttc gggtgtaacg gcgggatacc cctgcgcatc ttccggcacc tggtgcatag
    31141 ccggaatagg gaccagcggc cctaaaaaac gcggttcacg tttaaaaata taaaggtcag
    31201 ccagcgcacc gaaacgggcg gcaaattcgc gaatacgtac tgacagcata tctttcggtg
    31261 acggtacggc ataacactga gcctgaatcc ccatatgcag cgcaataaat aatgctcgct
    31321 cacagtggaa acgttgggtg ataataatga aatcattagt atcgaaaact ttgcgtgtac
    31381 gcacgatgga atccagcgta cgaaagcctg cgtaatcgag aacaatatct gatgggtcga
    31441 caccagcagc gattaaatct ttgcgcatgg tcatcggctc gttataactt tgcaatgcgt
    31501 tatcgccgct cagtaataga taatttacct taccgctgtt ataggcatta atcgctcctt
    31561 gaatgcggta gcgataatac tgattaataa cgccggtacg ataatatttt gctgtgccga
    31621 gcaccacgcc gacctggcgg taggggagat cctgcaattc gtcgtagata taaggcgcgg
    31681 ttttccagct catccagcga tcgaggccga gcacagtcaa cagcagcaag ccgatcagga
    31741 ctaacaggct gaggaacacg cgctttaaca tgcaggagac tcgagctaag gaaaatttcc
    31801 atcaggctac tttacccgct ggttttgcgc aagaaacggc aaactgtttg ccgggaattt
    31861 cgacaccagg agagggctgg caagccggac aaggcgttca cgccgcatcc ggcaccaggc
    31921 ttaagcgaga agcacgcggt cgatactgtg acagccgaga gctttcagcg ttgcggaggt
    31981 ggcatcccac tgaatcaacg gcgcatcggc tttttctgcc agaaccgcgc gctggatatc
    32041 cgggtaatca taacccttga gattcaacag aattaacgcc ccctgcaacg gcggcagagt
    32101 cgggttgaat gcggcgtttt cagcgtagct gccgctgaaa atacgaccgt ctttacattc
    32161 cagcgcgaca ccgcttggtg acttactgta aggcatgtgc gaacggtttg ccgctgcaat
    32221 cgccgcctga gaaagcgcat caccagtcag cgcatagccg tgatcctgct cgtccatcag
    32281 cagcgtttta atctccagat ctttcggccc aaaggcatcc ggcagatagt cacgcagcgc
    32341 gtgtgcctcg cggcccggca aatgaatacg cagatccaga ccgctgttca gttcattcat
    32401 aaactgacgg cagtgaccac aaggcgtata gttaacggtg atggctgcaa gcgctttttc
    32461 accactcaac caagcgtggc tgatcgcgct ttgttcggca tgaacggttt gctgcattgt
    32521 tgcaccaata aattccatat tggcaccaaa ataccaggtt ccgctcacac cgcgcgcaat
    32581 tgcgccaaca ttaaaattcg acaatggcgt acgcgcacag gccgccgcca gcggaagtag
    32641 tgcgaatgcc agcgcgtctt cgtccagtcc cgttgcgctc ttcagcgatg agacttgctc
    32701 cccggtcaac aaagcgggga agtacttgtc tgccagaata ggttccagtg cagattgcaa
    32761 gttatccgca agttgggcaa aagcggtttg aaaacgtgga tgcatggcgt tgcctcataa
    32821 tcatgtaatg ggataacagt ttacgaaccc gttatggctt tatatgtgat ctgaatctca
    32881 ttaattatgc aacacgtgaa acttacatca aaaatgcgcg acgtatcgca aattttagcc
    32941 cattactgcc agaataatcg ggaaaaggaa cggtgcgatc agcgaagtaa ttatcccgca
    33001 taacaccagc gctagcgaac taaatgcacc ttcctgataa tccagctcgg cgcagcgcgc
    33061 cgtaccgagg gcgtgcgagg cagtccccat tgccagaccg cgcgcagctt tggtacgaat
    33121 acgcatcgca ttaagcaatg tatggccaaa taccgcgccg aggatgccga cgaaaatcac
    33181 gcaaactgcg ctgattgccg gaataccgcc aatactgccg ccaaccgcca ttgcaatagg
    33241 cgtggtgact gatttcggca ggattgacgc ggcgatttcc ggtgaagcgc ccatcaacaa
    33301 tgccacggaa gtccccgtca ccatcgcaac cacgctgccg ataaaacaga tggtgatgat
    33361 cgatttccag cgcgcgcgga tctggtgtag ctgctcatat aaaggatagg ccagcgcgac
    33421 gaccgccggt tgcagcagat cgtttagcac ttcgctacct ttaaagtaac tgtcgtaaga
    33481 gatgccggtc agcattaaaa aaggaatgat caccaccatt gctaccagca acgggttaag
    33541 caggggaaac ttataccgtg ccgccagttt gcgggcggca aaaaagacaa tcaacgtcag
    33601 cggtaatgac caccagatat tcgccatcat tcttctgatc ctttctgacc tacgacttta
    33661 cgttcaccgt gtaccagttg tgaactccag ctcaccacca gaaaaaccac cagcgtactg
    33721 acagcacagg aaaccactac cgggccaaac tgcgcgcgga gcaaatcaaa atattgcatg
    33781 acgcctacgc caatcggcac aaacaatagc gccatatagc gaatcagtac gtagcacccc
    33841 ggattgaccc atttcgccgg aagaatttgc aaggccagca ggacaaacag gatcagcatc
    33901 ccgatgatgc tgcccggaat ggttaccggt agcagggaag caatgaaaat gcctgcatac
    33961 aggcaggcat aaatcaggac gaaagcgcgt aaatattgcc agataatgtt cagtgtctta
    34021 ctcataatca acaccccaga tgagatggat tcatcataca attaattcgt aaaacgtgct
    34081 accgatcaca ttaagaaatc agcgcttatt ttttcgctac ccggtattac gcaatgagca
    34141 gcgaactcag gcatacatca agcatagaga gggattgcag tttgcagcaa tccctctttg
    34201 cagaccttta atccgctgac cataggcgct aacatagcgc ctcatttttt tgcgggtgat
    34261 gatatgcgtg tgttactggc accgatggag ggagtgcttg actctctggt gcgtgaattg
    34321 ctgaccgaag ttaacgacta cgatctgtgc atcaccgagt ttgtccgcgt ggtggatcaa
    34381 ctgctgccgg taaaagtctt tcatcgcatt tgccctgagc tacaaaacgc cagccggaca
    34441 ccatctggta cgctggtgcg cgtgcagttg ttaggtcagt tcccacaatg gctggcagag
    34501 aacgccgccc gtgcggtgga gttaggtttc tggggcgtgg atctcaattg cggctgcccg
    34561 tcgaaaacgg ttaacggtag cggcggcggg gcgacgttac tcaaagatcc tgaactcatc
    34621 taccaggggg caaaagcgat gcgtgaagct gtaccggcgc atttgcctgt cagcgtgaaa
    34681 gtgcgtctgg gctgggacag cggtgagaag aaatttgaaa tcgccgatgc ggttcaacag
    34741 gctggcgcta cggagctggt ggtgcatggg cggacgaaag agcagggtta ccgcgcggag
    34801 catattgact ggcaggcgat tggcgagatt cgccagcggc tgaatattcc ggtgattgcc
    34861 aacggtgaaa tctgggactg gcagagcgcg caacaatgca tggcgatcag cggctgcgac
    34921 gcagtgatga ttggtcgcgg ggcgctcaat attcccaacc tgagccgggt ggtaaaatat
    34981 aacgaaccgc gaatgccgtg gccggaggtg gttgctttgc tgcaaaaata tacccgtctg
    35041 gaaaagcagg gcgataccgg gttatatcac gttgcgcgga ttaaacagtg gttgagttat
    35101 ttgcgtaaag aatacgatga agcaacggaa ttatttcagc atgttcgggt gttgaataac
    35161 tcccctgata ttgcaagggc tattcaggca attgatatcg agaaactcta aaaaccatag
    35221 tgatgttttt gacaatgtgt tagctcttta tcaataggcc gcacggttgt ttggtggcgt
    35281 caaaatcgcc gaggcgttcc ctgaaggccg gggcagccca catggatgtg ggctgagggc
    35341 gcgttttaca gggatgttac ctcgcgcccg acccggtagc cgtaagggat aagccgaggg
    35401 caccgcgcag cggcgatttt gttcgccaga gcccgggggg gcagggggcg gcggcgattg
    35461 gtcgccccct gcgcgctcct tgcgccagtg attatatgaa gcaatgcaca tgacaggagc
    35521 gcaacaggat gagagactga aatattctcg ttattttcgg atctgatgac attaattaat
    35581 tttaaaatat catcttaaat acgccagtca ccaccagtag cccaatcagg aaaataatca
    35641 atacagccca gagtataatt ttcattggcg ttcctttatt tgtgagtgct gaataagtat
    35701 aggaaaaaac gaagcccttt gccgttatcc ataaacgatt aagataaatc ggaaaatatc
    35761 atttacttag tctatatttt tctgggtatg tcgttttatt gctactgatg tattgccacg
    35821 cgaaactaaa aaatatatcg caacacacgg taatctccgc gcaccgtttc ttacctgatt
    35881 attcccccat gaatcgtgat tccttttatc cagccatcgc ctgttttccg ctgttactga
    35941 tgctggccgg gtgtgcgcct atgcatgaaa cccgccaggc gttaagccag caaacgcccg
    36001 ctgcacaagt tgacaccgca ttacccacgg cgctgaaaaa tggttggcca gacagccaat
    36061 ggtggctgga gtatcacgat aatcagctca cttcattaat taacaatgca ctacagaacg
    36121 caccggatat gcaggtggct gaacagcgca tttagctagc tgaagcccag gcgaaggcag
    36181 ttgccactca ggatggtccg cagatcgact tttcggcgga tatggagcgg caaaaaatgt
    36241 cggcagaagg gctaatgggg ccgtttgctc tgaacgatcc ggccgcaggt acgaccggtc
    36301 cgtggtacac caacggaact tttggcttaa cggcgggctg gcatctcgat atctggggaa
    36361 agaatcgggc ggaggttact gcccgcctgg gtacggttaa agcacgggcg gcggaacgcg
    36421 agcaaacccg ccaattgctg gctggcagcg tagcccgcct gtactgggag tggcaaaccc
    36481 aggcggcgtt aaacacggtc ttgcagcaaa tagaaaaaga gcagaacacc attatcgcga
    36541 ccgatcgcca gctatatcag aacgggatta cttcttcagt tgacggtgtg gaaaccgata
    36601 ttaatgccag caaaacccgg cagcagctca acgatgtcgc ggggaaaatg aaaattattg
    36661 aggcacggtt aaatgcactc acaaatcacc agactaagtc attgaagctt aaaccggtcg
    36721 cgttgccgaa agtggcaagc cagcttcctg atgaactggg gtactcctta ctggcccggc
    36781 gggcagattt gcaggcggcg cactggtacg ttgagtcatc gttaagcact attgatgcgg
    36841 caaaagcggc attttatcct gacatcaacc tgatggcctt cctgcaacag gatgcgttgc
    36901 acttaagcga tctgttccgt cattccgcgc agcaaatggg cgttacggca ggcctgacgc
    36961 tacctatttt cgatagtggt cgtcttaacg ccaatctcga tatcgcaaaa gccgaaagca
    37021 acttgtctat cgccagctac aacaaagcgg tggttgaagc agtgaatgac gtggcgcggg
    37081 cagccagtca ggttcagaca ctggcggaga aaaaccagca tcaggcgcaa attgagcgcg
    37141 atgccttgcg tgtggtaggt cttgcgcagg cccgctttaa cgcgggcatc attgctggtt
    37201 cccgcgtcag cgaagccaga atcccggcgc tgcgtgagcg ggccaatggc ctgttattgc
    37261 aagggcagtg gctggatgcc tccattcgac tcaccggtgc gctgggcggg ggttacaaac
    37321 gctgatgata tgcgccttct atacttaacg tttattcagc gttaagtgga gaactcgatg
    37381 gcataggtcg cgataattac cgcttccgat tcggggatcg gcaaagagtg cgcgttatta
    37441 ctggcgcagc aggggtttga tattggcatt acctggcact cagatgaaga aggggcaaaa
    37501 gataccgcgc gtgaggtggt tacccacggc gtacgtgcgg agatcgtgca actggatctc
    37561 ggcaatctgc cagaaggtgc gcaggcgctg gagacactca tccagcgatt tgggcgtatt
    37621 gatgtgctgg tgaataatgc gggtgcaatg accaaagcgc cgtttcttga tatggctttt
    37681 gatgagtggc gcaagatttt taccgttgat gttgatggtg catttttatg ctcgcaaatt
    37741 gcggctcgtc agatggtgaa ataagggcag ggcggtcgca tcatcaacat tacgtcggta
    37801 catgaacata cgccgctgcc ggatgccagc gcctacacag ccgctaaaca tgcgctcggc
    37861 gggttaacca aaacgatggc gctggagttg gtcaggcata agattttggt gaacgcagtc
    37921 gcgcctgggg cgatagccac gccaatgaac agcatggatg atagcgacgt gaagcccgac
    37981 gcggagcctt cgattcctct acggcgtttt ggcgcaacgc atgagattgc cagcctggtg
    38041 gtgtggcttt gttcggaggg cgcaaattac acgacagggc agtcgttgat agtggatggc
    38101 ggctttatgt tggcgaatcc gcagttcaac ccagaatagc gcagtgaggt gcgtggatat
    38161 ttcaatgggt taggccagat aagacaccag cgtcgcatca ggcatggaac acccggtgcc
    38221 ggatgtggag tgaatgctta tctgtggcaa ctgctttacg cctgattatc cggctttttc
    38281 ttcccgcgcc gtttcagcca ccagcgcacg cccaccacca gaaccacgac cagaatcaac
    38341 cagacccagt gcttcaaatg ctggtcgaga ttgtgcaacc acggcgcaat cacctgacca
    38401 ccagcgtaac caatagtggt aaaaatcaac gcccaggcaa atgcgccgag aatattcagc
    38461 ggcagaaaga ttttcggcgg cagctggctg gcaccaatca gcgtcgggcc aatcacccga
    38521 aagccataca taaagcgcgt accaatgaca aacagatacg gatggcgttg gataagtttc
    38581 tgtgcccgct caattttatc ctgatgtttc gagaaacggc gtaacagctt gccgccaaac
    38641 cgccgcccgc acagatagag cacctggtcg ccaatcatgc caccaagcgc cacagaaagt
    38701 accaccagtg ggaactttaa tagcccctga tgtgccgcaa cgcctcccag caaagtcacg
    38761 gtttcacctt ccgccaggct accgataacc agcgcggcat aaccatattg tgagatgagt
    38821 gtattgagat ccataggcca gtttgtctcc ctttggttgc ctgtaagcat acaccctgtg
    38881 aatcattcac gctgaaaaag gacatacatc agacggtgca aattacatgg atgaacaaaa
    38941 aataatcaca agctggctta tacttgaact gtcaggttca tgaactcaca acaagggggc
    39001 ggtatgagcc atgtctgggg actgttttcc catcccgatc gtgaaatgca ggtgattaat
    39061 cgcgaaaacg aaacgatttc tcaccattac acccaccacg ttttgctgat ggcggcgatt
    39121 ccggtgattt gcgccttcat tggcactaca cagattggct ggaattttgg cgatggcact
    39181 atcctgaagt tatcctggtt taccggactg gcgctggctg tcttgtttta cggcgttatg
    39241 ctggctgggg ttgcggtgat ggggcgggtc atctggtgga tggcgcgcaa ttacccgcag
    39301 cgtccgtctc ttgcgcactg catggtcttc gcgggctacg ttgcaactcc gctgttttta
    39361 agcggtctgg tggcgcttta tccactggtc tggttgtgtg cgctggtcgg cacggtggcg
    39421 ctgttttaca ccggctacct gctttacctt ggtattccga gcttcctgaa tatcaacaaa
    39481 gaggaaggcc ttagcttctc cagttcgacg ctggcgattg gcgtgctggt actggaagtg
    39541 ctgctcaccc taaccgttat actatggggc tacggttatc gtttgttcta gaaagttctt
    39601 tgacgttgca ttgctggcgt aaatgtcagc aatgcagcat ttactcacga ttctcctttg
    39661 acgatcgcac tttttgctcg ttatgatggc gtcgctagcc tcagtaaatc cttactccgg
    39721 cggtgcgcaa cccgtgcgcg tgaaccacta tctgaatgct catcatgccg aaatttcgag
    39781 tttctttatt tagcttggcc ctgatgctgg ctgtgccttt tgcaccgcag gccgttgcga
    39841 aaacggcagc cgctaccacc gcttcacaac cggaaattgc ctccggtagc gcgatgattg
    39901 ttgatctgaa taccaacaaa gtgatctatt cgaaccaccc ggatctggtg cgtccgattg
    39961 cgtctatcag caaattaatg accgcgatgg ttgtgctgga tgcacgactg ccgctggatg
    40021 aaaaactaaa agtggatatc agccagacgc cggagatgaa aggggtctat tcgcgcgtac
    40081 gactgaatag cgaaatcagc cgtaaagata tgctgttgct ggcgctgatg tcttcagaaa
    40141 accgcgcggc ggcaagcctt gcgcaccatt atcccggtgg ttacaaagcc tttattaagg
    40201 caatgaatgc gaaagcgaaa tcgctcggaa tgaacaacac gcgctttgtt gaacctaccg
    40261 gattgtcggt gcataacgtt tcaactgccc gtgacttaac caaactgctc attgccagca
    40321 aacaatatcc gttgatcggg cagttaagta ctacccggga agatatggca accttctcta
    40381 acccgacgta tacgctgccg ttccgcaata ctaatcatct ggtgtatcgc gataactgga
    40441 atattcagtt aaccaaaacc ggctttacca atgcggcggg ccattgtctg gtgatgcgta
    40501 cggttatcaa taataaaccg gtggcgctgg tagtgatgga cgcgtttggc aaatataccc
    40561 attttgccga tgccagccgc ctgcgtacct ggattgaaac cggtaaagtg atgcctgtgc
    40621 cggcagcagc gttgagctat aaaaaacaaa aagccgccca aatggcggcg gcggggcaga
    40681 cggcacagaa cgattaagtg aattcggatg gcgatactct gccatccgta atttttactc
    40741 cacttcctgc cagtttttcc gtttactggt tttaccgatc cccggattca tgctattggt
    40801 cggatcattc tcgcgataga acttctgcaa cgtctccggt gctttataca aatgaccgac
    40861 gttatgctcg gcagggtact gcgcgccgcg ctgctgtagc agttccagca tctgttcttt
    40921 taacgcatgc acatccacgc cttttttcac tatgtaatcc tgatggaaga catagcacat
    40981 aaaatggccg taatagagct tgtgcaccag ctggctgtcg atctccggcg gtaaatgctc
    41041 ataccactcg gtgtcgttac gccgcagagc gatatccagt gccagaatgt cttcgacttc
    41101 atcggaatgc accgcctgat aacgaattgc tgcgcccgca gcggcgaaac ggtgtaaaaa
    41161 cgctttgcta ccttcctccg gcgtacagac aaagaaatcg ccctcggcct gtttgaaata
    41221 atccaccagc cacgatttgg cttcaccgac gccatcgcct gccattttta acagcagatg
    41281 atgctcgtat ttatcgcgcc agtttttcat gcgcggcggt aaatggctgg ggaacaggtg
    41341 accgaatttt tgcattgcac ggtcagtaaa atgcggacgg aagaatttca ctttctccag
    41401 catcgcatcg gtgcgtccct tgagattaaa gaagaacggc atcttgtcgg tgcctaactt
    41461 atcaatcatc aggaaggtgt ctttgccgta tttttccgca atatcgtaga tatcccggtg
    41521 catatattcc ccggcaaccg gcagattttc gaagttagcc agaatatgac ggcggatttc
    41581 ggtcagcact tccggctggt tggtgccgat ataaaacacc tgctgatttt tttccgcttc
    41641 gaaggtatca agacgtaccg caaagacggc cagcttcccg gcgcaaccag aagattcaaa
    41701 taaccgatcc ggatcggcgt tataacgtgc gggcgtgtcg gcttcaatat cacgaacgcg
    41761 gtggacatag tcataatcgt gggcgtgacg accatcgtga cgcacatcgt catctttgat
    41821 gcgatcgtca tccagcttgc taaggatctg ctccggcgtt tcgcccagat caatccccag
    41881 atggttcacc agcgtcagtt tgccatcttc atctatacgc gcgaataacg acatttcggt
    41941 atacgccggg ccacgttgca ccagcgagcc gccggagttg ttacaaatac cgccgatgac
    42001 cgatgcgcct atacacgatg atccaatcac cgaatgcggt tcgcgtccca gcggtttgag
    42061 ggctttttcc agcgaataga gcgtggtgcc cggataggcc agcacctgtt cgcccttgcc
    42121 gagaacgtgc agcttgtcga gacgcagggt gctgatgatc acgatatcgc gatcatagtc
    42181 gttaccgttt ggcgtcgatc cttcggtcag gcctgtattg gcagcctgca tcagaataat
    42241 tttgtcggcg gtgacgcagg ctttcagcac ccgccacaat tccagtagtg agccagggaa
    42301 aacgacagcc agcgcgtcgc cctgaccaga acggaagccc ttgcgatagc gggccgtttt
    42361 tgcgggatcg gtgagcaggt gtgaatgacc gaccagacgg gcaagttcat tcaaaaaggc
    42421 tttattatca gttgttgtca tggaagacat tttccactcc ttgtggtggc gaaaaaatat
    42481 cacaaactag aatagcgcct tgtatgacaa ttcagcgagt gaatattttg aaaattcaga
    42541 caataaactg agcattcttt ttctctctcc atcatgctta tggcaaacta cggtttttgc
    42601 accattttcg ctgccacgtc gggcaacaaa ggaagaaaaa tccatatgaa atggctatgt
    42661 tcagtaggaa tcgcggtgag tctggtcctg cagccagcac tggcggatga tttattcggt
    42721 aaccatccat taacgcctga agcgcgggat gcgttcgtca ccgatctgct caagaaaatg
    42781 acggttgatg agaaaattgg tcagctgcgc ttaatcagcg taggcccgga taatccgaaa
    42841 gaggcgatcc gcgagatgat caaagacggt caggttgggg cgattttcaa caccgtaacc
    42901 cgtcaggata tccgcgccat gcaggatcag gtgatggaat taagccgcct gaaaattcct
    42961 cttttctttg cttacgacgt gctgcacggt cagcgcacgg tgttcccgat tagcctcggt
    43021 ctggcctcgt cttttaacct cgatgcggtg aaaacggtcg ggcgtgtctc tgcttatgaa
    43081 gcggcagatg atggcctgaa tatgacctgg gcaccgatgg tcgatgtctc gcgcgatccg
    43141 cgctggggac gtgcttccga aggttttggc gaagatacgt atctcacctc aataatgggt
    43201 aaaaccatgg tggaagcgat gcagggtaaa agcccggcag atcgttactc ggtgatgacc
    43261 agcgtcaaac actttgccgc atacggcgcg gtagaaggcg gtaaagagta caacaccgtc
    43321 gatatgagtc cgcagcgcct gtttaatgat tatatgccgc cgtacaaagc ggggctggac
    43381 gcaggcagcg gcgcggtgat ggtggcgctg aactcgctga acggcacgcc agccacctcc
    43441 gattcctggc tgctgaaaga tgttctgcgc gaccagtggg gctttaaagg catcaccgtt
    43501 tccgatcacg gtgcaatcaa agagctgatt aaacatggca cggcggcaga cccggaagat
    43561 gcggtgcgcg tggcgctgaa atccggaatc aacatgagta tgagcgacga gtactactcg
    43621 aagtatctgc ctgggttgat taaatccggc aaagtgacca tggcagagct ggacgatgct
    43681 gcccgccatg tactgaacgt taaatatgat atggggttgt ttaacgaccc atacagccat
    43741 ttggggccga aagagtctga cccggtggat accaatgccg aaagccgcct gcaccgtaaa
    43801 gaagcgcgtg aagtggcgcg cgaaagcctg gtgttgctga aaaaccgtct cgaaacgtta
    43861 ccgctgaaaa aatcggccac cattgcggtg gttgggccac tggcggacag taaacgtgac
    43921 gtgatgggca gctggtccgc agccggtgtt gccgatcaat ccgtgaccgt actgaccggg
    43981 attaaaaatt ccgtcggtga aaacggtaaa gtgctgtatg ccaaaggggc gaacgttacc
    44041 agtgacaaag gcattatcga tttcctgaat cagtatgaag aagcggtcaa agtcgatccg
    44101 cgttcgccgc aagagatgat tgatgaagcg gtgcagacgg cgaaacaatc tgatgtggtg
    44161 gtggctgtag tcggtgaagc acaggggatg gcgcacgaag cctccagccg gaccgatatc
    44221 actattccgc aaggccaacg tgacttgatt gcggcgctga aagccaccgg taaaccgctg
    44281 gtgctggtgc tgatgaacgg gcgtccgctg gcgctggtga aagaagatca gcaggctgat
    44341 gcgattctgg aaacctggtt tgcggggact gaaggcggta atgcaattgc cgatgtattg
    44401 tttggcgatt acaacccgtc cggcaagctg ccaatttcct tcccgcgttc tgtcgggcag
    44461 atcccggtgt actacagcca tctgaacacc ggtcgtccgt ataatgccga caagccgaac
    44521 aaatatactt cgcgttattt tgatgaagct aacggagcgc tttatccgtt cggctatggt
    44581 ctgagctata ccactttcac cgtctctgat gtgaaacttt ctgcgccgac catgaagcgt
    44641 gacggcaaag tgacggccag cgtgcaggtg acgaacaccg gtaagcgcga gggggccacg
    44701 gtagtgcaga tgtacttgca ggatgtgacg gcttccatga gtcgccctgt gaaacagctg
    44761 aaaggctttg agaaaatcac cctgaaaccg ggcgaaactc agactgtcag cttcccgatc
    44821 gatattgagg cgctgaagtt ctggaatcaa cagatgaaat atgacgccga gcctggcaag
    44881 ttcaatgtat ttatcggcac tgattccgca cgcgttaaga aaggcgagtt tgagttgctg
    44941 taatgcttta gtaagggcgc gacgtttgcg ccctttgcct gatgttcata tcttaacttg
    45001 ctgaattttc gccctttgta ggccggataa ggcgctcgcg ccgcatccgg caggaaacgc
    45061 cggaccgaat atgcctctta agtatttatc tgattctgca actgaatcct tccgctcagg
    45121 ctaactctgt cattatcaac tatgcttttc tcttaattcg ctgaaaacaa cagtaaaaga
    45181 atgaggaaag catcatgcca ctcttaaagc tctgggcagg ttcactggtt atgttggcag
    45241 ccgtgagcct gccgctacag gcggcttccc ccgttaaagt cggttcaaaa atcgataccg
    45301 aaggcgcgct gctcggcaac atcatcttgc aggtactcga aagccacggc gtaccgacgg
    45361 tgaataaagt gcaactcgga acgactcctg tggggcgcgg ggcgattact tccgatgaac
    45421 tggatatcta tccggaatat accggcaatg gcgctttctt ctttaaagat gaaaacgatg
    45481 cagcgtggaa aaatgccggg cagggctacg agaaagtcaa aaagctcgat gcggagcaca
    45541 acaagttaat ctggctgacg cccgcgcctg caaataacac ctggactatc gccgtgcgtc
    45601 aggatgtggc agagaaaaac aaactcactt cgcttgccga cctgagtcgt tatctgcaag
    45661 agggcggcac cttcaaactg gcagcctcgg cagagtttat cgaacgcgcc gatgcgttac
    45721 ccacgtttga aaaagcctat ggctttaagc tcggtcagga tcagttgctg tcactggctg
    45781 gtggcgacac ggcggtgacg attaaagccg ctgcccagca aacctctggc gttaatgccg
    45841 caatggctta cggcactgac ggtccggtcg cggcgctggg gctgcaaacc ttaagcgatc
    45901 cgcaaggcgt gcaacctatc tacgcgcctg caccagtggt gcgtgaatcg gtgttgaagg
    45961 agtatccgca aatggcacag tggctacagc cagtcttcgc cagcctcgat gcaaaaacat
    46021 tgcagcaact gaatgccagc attgcagtgg aaggactgga tgccaaaaaa gtggctgccg
    46081 actacctgaa acaaaaaggg tgggcgaagt aatttcccgt gacttatcta cgtattaatc
    46141 ctgttctggc gctgctgttg ttgctgacgg caatcgcagc ggcgctgccg tttatcagtt
    46201 acgcgcctaa tcgtttagtc tcgggtgaag ggcttcatct ctggcaactg tggccgcaaa
    46261 cgatctggat gctggtgggc gttggttgcg cctggctgac agcctgtttt gtccccgcta
    46321 aaaaaggcag catttttgca ctcattctgg cgcaattcgt cttcgtattg ttggtgtggg
    46381 gagctggaaa ggcggcgacc caactggcgc aaaatggcag tgcgctggcg cgtaccagcc
    46441 tcggcagagg tttctggctg gctgcggcgc tggcattgct ggcctgtagc gatgccatcc
    46501 gccgaatctc cacgcatccg ctgtggcgct ggttgttgca tatgcagatt gccattattc
    46561 cgctgtggtt gctgtactcc ggcacgctta acgatctctc actaatgaaa gaatacgcca
    46621 accgtcagga tgtgtttgac gacgcgctgg cacaacatct gacgttgctg tttggtgcgg
    46681 tgctgcctgc gttagtgatt ggtgtgccgt tgggcatctg gtgctacttt tccactgcgc
    46741 ggcagggggc aattttttct ctgctcaatg tcattcagac cgtgccttcg gtggcgctct
    46801 ttggcctgtt gattgcgccg cttgccgcgc tggttacggc ctttccgtgg ctggggaagc
    46861 tcggcatagc agggaccgga atgacacccg cactgattgc gctggtgctc tatgccttgc
    46921 tgccgctggt gcgaggcgtg gtagtcggtt tgaaccagat cccgcgcgat gtgctggaga
    46981 gcgccagagc gatggggatg agcggggcgc agcgtttcct gcatgttcag ttaccgctgg
    47041 cgttaccggt atttctgcgc agcctgcggg tggtgatggt gcaaactgta ggtatggcgg
    47101 tgattgcggc gttaatcggc gcaggcggtt ttggtgcgct ggttttccag gggctgctaa
    47161 gcagcgccat tgatttagtg ttgctggggg tgatcccggt aattgttctg gcggtgctta
    47221 ccgacgcgct gttcgatttg cttatcgcac tgctgaaggt gaaacgtaat gattgaattt
    47281 agccatgtca gcaaactgtt cggcgcacaa aaagccgtta acgatctcaa tctcaatttt
    47341 caggaaggga gtttttcggt gctgattggc acatctggct ccggcaaatc caccaccctg
    47401 aaaatgatta accgcctggt ggagcatgac agcggagtga tccgctttgc cggagaagaa
    47461 attcgctcgc tgccagtact ggagttgcgc cgccggatgg gctatgccat tcaatctatt
    47521 ggcctgttcc cccactggag cgtggcgcaa aacatcgcta ccgtgccgca attacaaaaa
    47581 tggtcgcggg cgcggattga cgatcgtatc gacgaattaa tggcgctact ggggctggag
    47641 tcaaatttgc gtgagcgtta tccgcatcag ctttccggtg gtcagcagca acgtgtggga
    47701 gtggcgcgtg cactggctgc cgatccgcaa gtcttactga tggatgaacc ttttggcgca
    47761 ctggacccgg taacgcgcgg cgcgttgcaa caagagatga cgcgcattca ccgtttgctg
    47821 gggcgcacta ttgtgctggt cactcatgat attgatgagg cgctacggct ggcagaacat
    47881 ctggtattga tggatcacgg tgaagtggtg cagcagggga atccgctgac gatgctgact
    47941 cgtccggcga atgattttgt ccgccagttt tttggacgta gtgaactggg ggtgcacctg
    48001 ctttcgttac gtagcgtggc ggattacgtg cgtcgcgaag aacgggcaga aggtgaggca
    48061 ctggcagaag agatgacgct acgcgatgcg ctctccctgt ttgtcgcgcg gggatgcgag
    48121 gtgctgccgg tggtgaacac gcagggccag ccttgcggca cgctgcattt tcaggatctg
    48181 ctggtggagg cgtaagcgta tgaagatgtt gcgcgatccg ctgttctggc tcattgctct
    48241 ttttgtggcg ctgattttct ggctgcctta cagccagccg ctgtttgctg ccttgttccc
    48301 acaactgcca cgacccgttt atcagcaaga aagttttgca gctctggcac tggctcattt
    48361 ctggctggtg ggaatttcga gtttgtttgc ggtgatcatt ggcacaggtg ccggaattgc
    48421 tgttactcgc ccgtggggcg cggaatttcg cccactggtg gaaactattg ccgccgttgg
    48481 acagactttt ccgcctgtcg cagtgctggc gattgccgtt ccggtgatcg gctttggtct
    48541 gcaaccagcg attatcgcct tgatccttta cggtgtgctg cccatcctgc aggtgacact
    48601 tgccgggctg ggagcgattg atcccagcgt gacagaagtt gcgaaaggta tgggaatgag
    48661 tcgaggtcag cgactgcgta aggtcgagct accgctggcg gctccggtga ttctggcggg
    48721 cgtgcgaact tcggtgatta tcaacattgg tacggcgacg atcgcctcaa cggtaggggc
    48781 cagtacgctg ggtacgccca tcatcatcgg gcttagcgga tttaataccg cgtatgtgat
    48841 ccagggggcg ttactggtgg cactggcggc gatcatcgca gaccgcctgt ttgaaaggct
    48901 ggtgcaggcg cttagccagc acgcaaaata aaggtataac ctgcgagcat gacgccacca
    48961 attccgccta acgccataaa caggaacagg gcgatgaccc caattttagc tatgcgcata
    49021 atgcactcct tatgttaacg aaaggattgt acagtaaagc gcatttgtta acgaatcatt
    49081 aaataccgag tgggaaaata tcatggcctt gttcttgcca actggtgagt tgctgctgtt
    49141 gggcggaggt tcgattttca ccgcaccaca ccagcaatgt acggccttcg aatagttcag
    49201 ggcgtagttg attgagcgag tgggcgagcg aggacatcaa tgcgccatcc ttgttgactg
    49261 gcaatccagc cctccagcca cagacgggtg gtatcctgaa tattccagcc aaccaccagc
    49321 gcatctttac cctgtttttt acgtgccgaa gccagacaaa tggcgatgta gttgatcagt
    49381 acgccgtcga ggatcgccag cagcgcctgg agagtcggtt gttggcactg aagccgtcgg
    49441 cgcagaggaa taaacagatg tgtggtgagt gtctgggcgg ggtaatcctg accgcgctct
    49501 ttgatccacg ttcgcaggct atgtagattg
//
LOCUS       DAJTRD010000022        48329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTRD010000022 DAJTRD010000000
VERSION     DAJTRD010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.022"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            187..480
                     /locus_tag="OO315_001378"
     CDS             187..480
                     /locus_tag="OO315_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5890348.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO315_001379"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO315_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5890349.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="OO315_001380"
     CDS             complement(1922..2374)
                     /locus_tag="OO315_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5890350.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO315_001381"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO315_001381"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5890351.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="OO315_001382"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="OO315_001382"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5890352.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO315_001383"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO315_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5890353.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO315_001384"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO315_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5890354.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO315_001385"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO315_001385"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5890355.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO315_001386"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO315_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5890356.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO315_001387"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO315_001387"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5890357.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO315_001388"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO315_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5890358.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO315_001389"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO315_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5890359.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="OO315_001390"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="OO315_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5890360.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="OO315_001391"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="OO315_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5890361.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="OO315_001392"
     CDS             complement(15085..15582)
                     /locus_tag="OO315_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5890362.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="OO315_001393"
     CDS             complement(15579..15941)
                     /locus_tag="OO315_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5890363.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="OO315_001394"
     CDS             complement(15931..16278)
                     /locus_tag="OO315_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5890364.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="OO315_001395"
     CDS             16387..16800
                     /locus_tag="OO315_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890365.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="OO315_001396"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="OO315_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="OO315_001397"
     CDS             complement(18324..20039)
                     /locus_tag="OO315_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5890366.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVIITFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="OO315_001398"
     CDS             20206..21099
                     /locus_tag="OO315_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5890367.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="OO315_001399"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="OO315_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="OO315_001400"
     CDS             complement(21910..23526)
                     /locus_tag="OO315_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5890368.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFLEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="OO315_001401"
     CDS             23821..24495
                     /locus_tag="OO315_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5890369.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="OO315_001402"
     CDS             complement(24534..26195)
                     /locus_tag="OO315_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5890370.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="OO315_001403"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="OO315_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5890371.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="OO315_001404"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="OO315_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5890372.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27338..27410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27649..27981
                     /locus_tag="OO315_001405"
     CDS             27649..27981
                     /locus_tag="OO315_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5890373.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29221)
                     /locus_tag="OO315_001406"
     CDS             complement(27983..29221)
                     /locus_tag="OO315_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5890374.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTNTWTPDSQWLVFDVRPSGAS
                     FTGETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFH
                     HRRGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLD
                     PALDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGW
                     VGNHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVV
                     QRRLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKL
                     THNKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSP
                     DGQWLAWMEGGQLWITETDR"
     gene            29322..30422
                     /gene="cbrA"
                     /locus_tag="OO315_001407"
     CDS             29322..30422
                     /gene="cbrA"
                     /locus_tag="OO315_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5890375.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30383..31720)
                     /locus_tag="OO315_001408"
     CDS             complement(30383..31720)
                     /locus_tag="OO315_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5890376.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALCFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31795..32943)
                     /gene="dgoD"
                     /locus_tag="OO315_001409"
                     /pseudo
     CDS             complement(31795..32943)
                     /gene="dgoD"
                     /locus_tag="OO315_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32940..33557)
                     /gene="dgoA"
                     /locus_tag="OO315_001410"
     CDS             complement(32940..33557)
                     /gene="dgoA"
                     /locus_tag="OO315_001410"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5890377.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33541..34419)
                     /gene="dgoK"
                     /locus_tag="OO315_001411"
     CDS             complement(33541..34419)
                     /gene="dgoK"
                     /locus_tag="OO315_001411"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5890378.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34416..35105)
                     /gene="dgoR"
                     /locus_tag="OO315_001412"
     CDS             complement(34416..35105)
                     /gene="dgoR"
                     /locus_tag="OO315_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5890379.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35383..36039
                     /locus_tag="OO315_001413"
     CDS             35383..36039
                     /locus_tag="OO315_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890380.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36085..36897)
                     /gene="yidA"
                     /locus_tag="OO315_001414"
     CDS             complement(36085..36897)
                     /gene="yidA"
                     /locus_tag="OO315_001414"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5890381.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37011..37409)
                     /locus_tag="OO315_001415"
     CDS             complement(37011..37409)
                     /locus_tag="OO315_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5890382.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37649..40063)
                     /gene="gyrB"
                     /locus_tag="OO315_001416"
     CDS             complement(37649..40063)
                     /gene="gyrB"
                     /locus_tag="OO315_001416"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5890383.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40092..41165)
                     /gene="recF"
                     /locus_tag="OO315_001417"
     CDS             complement(40092..41165)
                     /gene="recF"
                     /locus_tag="OO315_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5890384.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41165..42265)
                     /gene="dnaN"
                     /locus_tag="OO315_001418"
     CDS             complement(41165..42265)
                     /gene="dnaN"
                     /locus_tag="OO315_001418"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5890385.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42270..43673)
                     /gene="dnaA"
                     /locus_tag="OO315_001419"
     CDS             complement(42270..43673)
                     /gene="dnaA"
                     /locus_tag="OO315_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5890386.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43970..44050
                     /locus_tag="OO315_001420"
     CDS             43970..44050
                     /locus_tag="OO315_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890387.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44280..44420
                     /gene="rpmH"
                     /locus_tag="OO315_001421"
     CDS             44280..44420
                     /gene="rpmH"
                     /locus_tag="OO315_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5890388.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44437..44796
                     /gene="rnpA"
                     /locus_tag="OO315_001422"
     CDS             44437..44796
                     /gene="rnpA"
                     /locus_tag="OO315_001422"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5890389.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44760..45017
                     /gene="yidD"
                     /locus_tag="OO315_001423"
     CDS             44760..45017
                     /gene="yidD"
                     /locus_tag="OO315_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5890390.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45020..46666
                     /gene="yidC"
                     /locus_tag="OO315_001424"
     CDS             45020..46666
                     /gene="yidC"
                     /locus_tag="OO315_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5890391.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46772..48136
                     /gene="mnmE"
                     /locus_tag="OO315_001425"
     CDS             46772..48136
                     /gene="mnmE"
                     /locus_tag="OO315_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5890392.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11988 a        13062 c        12220 g        11059 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt aatgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccag gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtattggtat
    29161 tggtattggt attggtattg gtgagcaggt gattacgggg agcaaaggtt atctgtttca
    29221 tggcgcggtc ctgatacaaa acggttcccg ccatcatact tcacaaggac ttcactttca
    29281 gacaatagtc ttttgccatc gtgatgaatg tgagtgaaaa gatggaacat ttcgacgtgg
    29341 cgattattgg cctcggcccg gcagggtcgg cgttggcacg aaagttagcc ggcaaaatgc
    29401 aggtgatcgc gctggataaa aagcaccagt gcggtactga aggtttcagc aaaccttgcg
    29461 gcggtctgct ggcaccggac gcgcagcgat cttttattcg cgatggactg acgcttcctg
    29521 tcgatgtgat cgccaatcca cagattttca gcgtcaaaac tgtcgacgtc gccgcatcgc
    29581 tcacgcgtaa ctaccagcga agctatatca atattaatcg ccatgctttc gacttgtgga
    29641 tgaaatcgct gatccccgcc agcgttgagg tttatcacga tagcctgtgc cgaaaaatct
    29701 ggcgtgagga tgataaatgg catgtcattt ttcgtgcaga cggctgggag caacatatta
    29761 ccgcccgcta tctggtcggt gccgatggcg caaactcgat ggtgcggcga catctctacc
    29821 cggatcatca aatccgtaaa tatgtcgcta tccagcagtg gttcgcagag aaacatccgg
    29881 tgccgttcta ctcctgcatc tttgataatg cgataactga ctgttactca tggagtatca
    29941 gcaaagatgg ttattttatc tttggcggtg cctatccaat gaaagacggt cagacgcgtt
    30001 tcacgacgct gaaagagaaa atgagcgcct ttcagttcca gtttggcaaa gcggtgaaaa
    30061 gtgagaaatg cacggtgttg tttccctcac gctggcagga ttttgtctgt ggtaaggaca
    30121 acgcctttct gattggtgaa gcggcgggat ttatcagcgc cagctcgctg gaggggatta
    30181 gctatgcgct ggatagcgca gagattctgc gtgcggtgtt cctgaaacag ccggagaaga
    30241 gcaacgccgc ttactggcgc gccacacaca aactgcgttt aaaactcttc ggcaagatag
    30301 taaaaagccg atgcctgacc gcaccggctt taagaaagtg gattatgcgc agtggtgtgg
    30361 cgcatattcc acagttgaaa gattatccaa cgcgcttcac atcacccacc agcaggatgt
    30421 aagagagcgc gccaatcaac gcgacggcgg agatataaac cagtgcaggc gcgaaaccgt
    30481 aaccctgcgc caggtacccc accaccagcg gaacggtgat gccgcccaga ccaccggcga
    30541 agttaaacac gccgccggtt aaaccaatca ggcgcatcgg tgccagagaa gagaccagcg
    30601 accaggtaat cgaagcaaaa ccgttgccga agaatgccag cgccatcagg cacataatca
    30661 tcatcggatc gttggtgtag ttagcgccca taatgcaggt ggagatcagc aagccgcaga
    30721 taatcggcgt tttacgcgca aagcccagtg aaaagccctt acgtaccagc agatccgcga
    30781 cccagccaga gagcaggacg ccgacaaacg ccgcgaggaa tggcaccgtg gtcataaagc
    30841 ctgctttcag cgccgtgatt cctttttcct gggttaaata gttcgggaac caggttaaga
    30901 aaaaacacag tgcagaagcc accgcaaatt gcccaagata aacgccgatc agtttacgat
    30961 ggaacaccag tttccagtct ttggctgtta acggctgacg cgcctctttc ttcaccggcg
    31021 catcaccatc caccagaccg ccgccatcac gaatgtaatc cagttcagct ttgctgatac
    31081 ctttggtcag gcgcggcggc tgataaacct taaaccaaat cagcgaccag ataatgccga
    31141 taccaccagt gacaatgaac acccagtgcc agctcaacat ctcctgaatc cagatcagca
    31201 gcggagtcaa aaacgccaga ccgacaaact gaccagacgt ataaaaacca acggcggaag
    31261 cgcgttcatg ttccgggaac cagctggtca ccatccggtt attggtcggg aaagcgggcg
    31321 cttcgaaaat accggttatc gcacgcagac caattaatga cattaatccc gtggcaaagc
    31381 cttggaataa agtcgccact gaccaaccaa atatcgcaat aaaataagtc acgcgagaac
    31441 ctacgcgatc taaaaaccaa ccgccgggga tctggcatag cgtataaagc caggcgaagg
    31501 ccgaaaatac atagcccatt tccgctttcg taatgccgaa ctcttcctga atatgggcgg
    31561 aagccacggc caggttagcg cggtcaacat aacaaatgac caccgtaata aagatcatca
    31621 ccagcgtcag ataacgccga cgccccggct ttgctgcatt aacgggaata tccatagcga
    31681 gctttctcca gattttgggc atagcgaagc cgctcaccat gccctgtaat ttacagaggg
    31741 ttatattttt gtattgctgt tttagtgccc gatgaggggc ttacgtggca ggaattacca
    31801 ctctgctacg ctgttatctt catgacgcca gagcggatta cgccagtccg gggcattttt
    31861 actgaactca ataactttag cttcgtcgat ttccacacct aagcccggtt tcgttaacgg
    31921 tttaaagaag ccgccaacca tgctgaagtc ttctttgttt ttcacaaagt cgagtaactc
    31981 cgcgcctttg ttgtaatgaa tgcccatact ttgttactga agtaccgcgt tataggaaac
    32041 aaagtcgata tgcaggcaag ccgccagtgc aatcggtccg aacggacagt gcggcgcaag
    32101 ggtcacatca taggcttctg ccattccagc aattttgtag cattcggtaa taccgcctgc
    32161 atgggagaga tccggttgca gaatcgaaat accgcctgcc tccagcacgc gtttaaaatc
    32221 gaaacgtgag aacatgcgct cacccgccgc cagtggaata tgcgtttgtg ccgccaattt
    32281 cgggtagtat tcggcttgtt ccgccagcac cggctcttca ataaacagcg ggcgatacgg
    32341 ctccagttct ttaatcagca ctttcgccat tggcgcgctg acgcgaccgt ggaaatcaag
    32401 accaaactca atctgattgc caaaagcttc acgaatttgt gccacggtgt tgactgccgc
    32461 atctaccgcg cgggagttat caattagccc cagttcttca caaccgttca gtttgaaggt
    32521 atcgaagccg atttcgcgca gggttttaat gccgtcgata acatccgccg gacgatcgcc
    32581 gccgacccaa ctgtaggctt taattttgtc gcgaaccagg ccgcccatca gttgccagac
    32641 tggcgcattc agcactttgc ctttgatatc ccataacgcc tggtcgatcc cggcgatggc
    32701 gctcatcagg attggaccgc cacgataaaa tccggcgcga tacatcactt gccataagtc
    32761 gttgatgcgc gaaggatcct gaccaatcaa atagtcaccc agctcgtgaa ctgccgcttc
    32821 caccgtacgg gcgcggcctt caatcacggg ctcgccccaa ccgaccacgc cttcatcggt
    32881 ttcaattttc aggaacatcc agcggggagg taaacgatac gtggtaattt tggtgatttt
    32941 cattgcactg cctctcgata cgccttaaca aatgctgctg cctgctgcgc ggtgcgttct
    33001 acggactgcc cggcgcgata aagatcgctg cctaagcccg ccccaacaca acctgcgtct
    33061 atccactgcg ccaggttttc tggcgtcacg ccgccaacgg caaagactgc gatgtccggt
    33121 ggtaataccg cttttaacgc tttgatgtat tgcggaccaa aagccgatga cggaaatatt
    33181 ttcagcgcct gcgcgcccgc ttcgagcgcg gtaaaggctt cggtcgccgt cgcgcagccg
    33241 gggcaaacgg tcatgccgta gcccaccgcg cggcggatca cttcactatg gatattgggc
    33301 gtaacgatga gctgacagcc catcctggcg agcgcatcga cctgttcagg tttcagtacc
    33361 gtacctgcgc caatcaacgc cttgtcgcca tacgcatcaa cgatggcggg aatgctctgc
    33421 tcccattgtg gggaattcag cgggatttca accgcgtcga acccggcgtc aatcaccgcg
    33481 ccaacatgcg ccagcgcctc gtcgggcgta atgccgcgca aaatggcgat cagcgggagt
    33541 ttagtttgcc actgcatgag cgatgctcct tataccagcc tgaaatgccg tgtcgcccgc
    33601 caccgccgcc acgtcgcaac ccatcgcctg aaaggcttgc tggtagcgcg cggtcagcga
    33661 tgttccggcg acaagggtga tggcgtgttg atgggtcaca tagtcgcgca tactggcaac
    33721 ctctgcgcca atcaacaaac cagagagaaa ttcgctgacc tgttcgcggg gaagtgttcc
    33781 cagcacatgc gaggcgcgaa cttcaaaaag ctgcggcaat atggcgggcg cattaaggcc
    33841 gcgctcaagg ccagccgcga aggcatcggc agagttttcc tgcggcggca aacctgcgcc
    33901 aatcaatgag tgatttaaca gtaaatgatg taattcaccg gtcatcacgg tgcgaaaatc
    33961 gttgatttgc tggctatcgg cctgcaccca tttgcaatgc gtaccgggca taacataaag
    34021 agaggaagga gccagagctc gcgcgccgat caattgtgtt tcttcgccgc gcatcacatt
    34081 gtggttatcg tcatgagaga cgcataatcc gggaataatc cagatattgt cgccaacaga
    34141 cgttaattgt tcgccaatag acgaaaaacg ggcaggaaca gataaatacg gagcaacttt
    34201 ccagccgaca ttgctgccga ccattcctgc catcaccact ggcgtatttt cttcacgcca
    34261 gtcggtcgtg acttctgcta acactgcagc cggagatttt ccgttcaggc gcgtgacgcc
    34321 tgcttctgat tgcctgctct ccaggcagtg gtcgccctga taaagccagg cgcgcagatt
    34381 ggtcgatccc cagtcaattg cgatgtagcg agctgtcatg tgatttcctt taaccttcgt
    34441 gtcgagctgg caatcatggt aagcgccgcc tgctctgccg catcgccgtc ctgatgccgt
    34501 atcgcatcga acagcgcctt atgttcctgg agcgtttgcg gcatgttggc ctcatcgccc
    34561 atccaggttc gttcaaaaac cgcccgctgc agcgaactga tcgcaatgct aagttgctgt
    34621 aacaccgggt tatgcaccga ctgcagcacc gcctcgtggt agcgaatatc cgcttcgtta
    34681 aacgcttcgc ggtcctgatt gttggcaatc atctcgttca gcgccgattc aatctgcgcc
    34741 agatcgctgg aggtcgcgcg ctctgctgcc caacgggcaa tcgccggttc caccagattt
    34801 cgcacttcgc tcatggcact gataagccgt gggtcgtagt cgttttccag cacccattgc
    34861 agtacgtcag tgtcgaggta attccactgg ttacgcggtg ccacaaacgc cccgcgataa
    34921 cgtttcattt caatcagccg cttcgccatc agcgaacgga acacctcacg gatgatgttg
    34981 cgcgaggttg caaactcttc acagagctcc gcctcagccg gaagtggcga gccaggtacg
    35041 tatttgccgt gaacgatctg tttacccagc gtaatgacaa tgcgatcggt tttattgaga
    35101 gtcatggaga gtccttgtgc tctgtatgtt cttctctact ttaccccgat cgatgcataa
    35161 cgcggcaact ttgtagtacc agcgtgatga cgttcgcgtt tgccgcgcgc gtaatgtagt
    35221 acaaacttat attattgcgc tacaatttag atcaaaaaaa gaacaacaca taaaaaaatg
    35281 acatgcgtcg ggcagaaatc tgaaaaggga tatcaggcgc taaacaggag ggaaagaaga
    35341 gtatgctttc aacggcttag ctactcgttt aaaggattaa tcatgaagtt gaattttaag
    35401 ggatttttta aggctgccgg tttattccca ctggcgctga tgctttcagg ctgtatctcg
    35461 tatgctctgg tttcccatac cgcaaagggt agttcaggaa agtatcaatc gcagtcagac
    35521 accatcactg ggctatcgca ggcaaaagat agtaatggaa caaaaggcta tgtttttgta
    35581 ggggaatcgc tggattacct tatcactgat ggtgccgatg acatcgttaa gatgctcaat
    35641 gatccagcac ttaaccggca caatattcag gttgccgatg acgcaagatt tgttttaaat
    35701 gcggggaaaa agaaatttac cggcacaata tcgctttact actactggaa taacgaagaa
    35761 gaaaaggcac tggcaacgca ttatggtttt gcctgtggtg ttcaacactg taccaggtca
    35821 ctggaaaacc taaaaggcac aatccatgag aaaaataaaa acatggatta ctcaaaggtg
    35881 atggcgttct accatccatt taaagtgcga ttttatgaat actattcacc cagaggcatt
    35941 ccggatggtg tttccgcagc attactgcca gtgactgtta cactggacat cattactgca
    36001 ccgctgcaat ttctggttgt atatgcagta aaccaataat cagtaagcgg gcaaacgcgt
    36061 ttatgctgtt tgcccgccca cagattaatt cagcacatac ttctcaatag caaacgccac
    36121 gccatcttca aggttggatt ttgtgacaaa gttcgccact tctttcaccg aaggaatagc
    36181 gttatccatc gccacaccga cgcctgcata ttcaatcatt gcgatgtcgt tttcctgatc
    36241 gccaatcgcc atgatttctt ccggtttaat acctaacacg tcggccagtg atttcacacc
    36301 cgtacctttg ttaacgcgtt tatcgaggat ttcgaggaag tacggcgcac ttttcagcac
    36361 ggtatatttc tctttcactt cctgcggaat acgcgcgata gcctggtcga ggatggcggg
    36421 ttcatcaatc atcatcactt tcaggaactg ggtattgggg tccattttct ccgcttcgca
    36481 gaacaccagc ggaattgtgg caacgaagga ttcatgcacc gtgtagtagc tgatatcgcg
    36541 gttagcggtg tacagcgtgg tgcggtccag ggcgtggaaa tgagaaccga cttcgcgaga
    36601 gagtttttcc aggaaacgat agtcgtcata gctgagagcg gtttgcgcca cggtgctacc
    36661 atcagcggcc ttctgtacca gcgcgccgtt ataagtaatg cagtagtcgc ccggctgttc
    36721 catatgcagc tctttcaggt agttgtgcac acctgcatac ggacgacccg tcgttagcac
    36781 gacattcacg ccacgggcgc gagctgcggc aatcgcattt ttaacggcgg gtgaaatggt
    36841 gtgatcgggc agcagaaggg tgccatccat atcgatagca atgagtttga tagccatgag
    36901 ttcccaaggt agattagttc ctgacccatg ctaacgcgat tccgctcaaa aatcagtaca
    36961 acacccgagg gaaaagggga tgcaacgcgg gttgcatgct ccccatttgc ttagcggaag
    37021 agtttccctt tcagcagttc catgcctgcg gaaagcagat cgttattggc ttgtgctgac
    37081 acttcacctt gcggtgagag cgcatcaata atcttcggca attgctctgc cagtaaactg
    37141 gaagccgtac tggtatccac gccaagtttt tgcccgagat cggacaccgc atttgtgccg
    37201 agcgccgact ccagttgctc gccgctaact gattgatttc tctgttgatt actcaaccag
    37261 gttgagagaa tggcccctaa gccgccactt tgcagttttt ccagcagcac ctgaatgcca
    37321 ccctgctcct caacccaact taaaatcgcc tgatatttcc ccgcatcgcc tttcagaaag
    37381 gcaccgacaa cttcatcaaa aagccccatg ataatcacct gtaaagcgtt acgtgttgac
    37441 ccaaaaagta tagatctgcg ggatgataat tgcggattgc agaaataaaa agggcggaga
    37501 tgatctccgc ccttttctta tagcttcttg ccggatgcgg cgtgaacgcc ttacccggcc
    37561 tacaaaatca tgcaaaatca atatattgca ggagtctcgt aggactaata agcgtagcgc
    37621 atcaggcacg ctcgcatggt taacgccatt aaatatcaat attcgccgct ttcagggcgt
    37681 tctcttcaat aaacgcacga cgcggttcta cggcgtcgcc catcagcgtg gtgaacaact
    37741 ggtcagcggc aatcgcatcc ttaacggtaa cgcgcagcat acgacggctt tccgggtcca
    37801 tggtggtttc ccacagctgt tccgggttca tctcgcccag acctttataa cgctggatgg
    37861 agaggccacg acgggactct ttcaccagcc agtccagcgc ctgctcgaag ctggctaccg
    37921 gctgacgacg ctcgccacgt tcgataaacg catcttcttc cagcaagcca cgcagtttct
    37981 cacccagcgt gcagatacga cgatattcgc caccggtgat aaactcgtga tccagcggat
    38041 agtcagtatc cacaccgtgg gtacgcacgc gaacaatcgg ctcgaacagg ttttgttcgg
    38101 cattggtgtg gacatcaaac ttccactggc tgccgtgctg ttctttgtcg ttcagttcgc
    38161 tgaccagcgc gttcacccag cgggtaacgg tctgctcatc agaaaggtca gcttccgtca
    38221 acgtcggctg atagataagc tctttcagca ttgctttcgg ataacgacgc tccatgcggt
    38281 tgatcatttt ctgcgtcgcg ttgtactcag ataccagttt ctctaacgct tcgccagcca
    38341 atgccggtgc actggcgttg gtgtgcagcg ttgcgccgtc cagcgcgata gagatctggt
    38401 actgatccat cgcttcgtcg tctttaatgt actgttcctg cttgcctttc ttcactttgt
    38461 acagcggcgg ctgagcgatg tagacgtgac cgcgttcaac gatttccggc atctgacgat
    38521 agaagaaggt caacagcagc gtacgaatgt gcgagccgtc gacgtccgca tcggtcatga
    38581 tgatgatgct gtgataacgc agtttgtccg ggttgtactc gtcacgaccg ataccacagc
    38641 caagcgcggt gataagcgtc gccacttcct gagaagagag catcttatcg aagcgcgctt
    38701 tctcgacgtt aaggatttta cccttcagcg gcagaatcgc ctggttcttg cggttacgcc
    38761 cctgcttcgc agagccgccc gcggagtccc cttccaccag gtacagttcg gaaagcgccg
    38821 gatcgcgttc ctggcagtct gccagtttgc ccggcaggcc agctaagtcg agcgcacctt
    38881 tacggcgggt catttcacgc gcgcgacgcg ccgcttcacg ggcacgggca gcatcgataa
    38941 ttttgccaac cacgattttc gcatcagttg ggttttccag caggtattcc gccagcagtt
    39001 cgttcatctg ctgttcaacc gccgatttca cctcagaaga aaccagtttg tctttggtct
    39061 gggaggagaa tttcgggtcc ggcactttta cggaaacgac cgcaatcagg ccttcacgcg
    39121 catcgtcacc ggtggcgctg actttggctt ttttgctgta gccttctttg tccatgtagg
    39181 cattcagggt acgggtcatc gccgcacgga agcctgccag gtgagtaccg ccgtcacgct
    39241 gcggaatgtt gttggtaaag cagtagatgt tttcctggaa gccatcgttc cactgcagcg
    39301 ccacttcaac gccaataccg tctttttcgg tggagaagta gaagatattt gggtggatcg
    39361 gcgttttgtt cttgttcaga tattcaacga acgctttgat gccgccttca tagtggaagt
    39421 ggtcttcttt gccgtcacgc ttgtcgcgca gacgaatgga aacgccggag ttgaggaacg
    39481 acaactcacg cagacgtttc gccagaattt catattcgaa ctcggtcaca ttggtgaagg
    39541 tttcgaggct gggccagaaa cgcaccatgg tgccggtttt ttcagtctcg ccggtaaccg
    39601 ccagcggagc ctgcggtaca ccgtgttcgt agatctgacg gtgaatttta ccctcgcgct
    39661 gaataaccag ctccagtttt tgcgacaggg cgtttactac cgaaacacca acgccgtgca
    39721 gaccgccgga cactttatag gagttatcgt caaatttacc gcctgcgtgc agaacggtca
    39781 tgatcacttc cgccgccgat acgccctctt ccgggtgaat accggtcgga atgccgcgcc
    39841 cgtcatcctg tacagagaca gagttatcgg cgtgaatggt gacgataatt tctttacagt
    39901 gacccgcgag cgcttcgtcg atagcgttat ctaccacctc gaataccatg tggtgcagac
    39961 cggtgccgtc atccgtgtcg ccgatataca tacccgggcg cttacgcacc gcatccagcc
    40021 ctttcaggac tttgatactg gaggagtcat aagaattcga catcaacgtt tctcgctcat
    40081 ttatacttgg gttaatccgt tattttaccc ttttccacgg taaacatctt cgaattttcg
    40141 tccgacatgt ctataacgtg ttcagcactg atcgcgctga caaagacctg tgattgcgtc
    40201 gcttttaagc ggctggcaag caacccgcga cgctcatcat caagctcaga ggcaaaatca
    40261 tctatcaggt agagacaccg ccgcccgctt tcacgagtga ggaactctcc ttgcgccaga
    40321 cgtaaggcgc acatcaacag cttaagctgc ccacgcgata aggtatcttc caccggcgca
    40381 ccgtcggcgc gaatgcgtaa gtccgcttta tgcgggccat gcgcggtgta ggttagctgg
    40441 cgatcgcgtt caaaattacg ttccagcacc tcagcgtact ctgtctcttt ctcccagccg
    40501 cgctggaaag agaaagtcag agaaaactca gggagaaatt gcttacaggt atcatccata
    40561 tcggccgcga taccggcgct atactccgcg cgccaggtgc tgatttgctc cgccagcggg
    40621 atcagttctt tatcccacgg gcgtagctgt tcgtaacgag tcacctggcg cagcgccgca
    40681 ttgcgctgct tgagcaatcg cttgagattg ctccaggcgg tgaaaaatcc aggttcgttg
    40741 tgaaagcatc cccagtcgag gaatgctctt ctgtatttgg ggccgccgtt gagtaaagta
    40801 aacccttctg gcgttatcaa ctgcatcggc atcaggtgcg ccagttccgc gactttatgc
    40861 ccgtccgtac cgtcaatacg gactttgctg tcgccctgtt tgtctttggt taagccaatc
    40921 gccgtctcgc gctcttcgcc ctgtaatcgc ccgtggagaa caaatgcctc ctgctcatgg
    40981 cgaatgacgc gaccaatctg caaactgcga aacgcccgac catggccgag cgtatagatg
    41041 gcttccagca cgctggtttt gccactgccg ttggcaccta ccagaaagtt aaagccggga
    41101 gataaagcga gatccgcggt ttcaatattg cggaaatcgc ggatcaacaa gcgggtgagg
    41161 gacattacag tctcattggc atgacaacat aggcagccga ctgtgatgcg gcatcttcaa
    41221 tctgcacgct ggaaaccgaa tcggtcagca tcatgcggac gttttcgcat ttcagcgcgt
    41281 tcagaacatc cagcacatag ctgacgttga agccgatttc catctccgca ccgctatagg
    41341 taacgtcgag gatctcttcc gcttcttcct gttccgggtt gttggcagta attttcagct
    41401 ggttttcgct gacatacagg cgcacaccgc ggaatttctc gttagagaga atcgccgcgc
    41461 gagcaaacgc ctgcttgagc agatcgcagc ctgcttccag atgtttgtcc gggttcttcg
    41521 gcagaacgcg acggtaatcc gggaagcgac catccaccag tttcgaggtg aaaataaagt
    41581 cgccaacgtg ggcacgaatg ttattgctgc caatctgcac acgcagcaga ttgtcgccgc
    41641 cgtcgagcat acgcatcagt tcaatcacgc ctttacgcgg tacgatcacc gaatggcttg
    41701 gcaaagattg accaattggc attgaacaga ccgccagacg gtggccgtcg gttgccacgg
    41761 tgcgcagttc ttcaccttcg gtttcaaaca gcataccgtt taagtaatag cgaacatcct
    41821 gatgcgccat cgaaaactgg gttgcttcaa tcagacgctt catcgtcgcc tgcggcaggg
    41881 taaattcgac ttcgctctgc cagtcatcga ggttcgggaa atccgccgct ggcagggtag
    41941 acagcgaaaa acggctacgc ccggagcgca ccagcatccg ttcaccttcc agctgcacgg
    42001 caatttccgc gccttcaggc agaccacggc agatatcaaa gaatttgcgc gccggaacgg
    42061 tcgtcgctcc tggctcgtgt ggctgaacca gcgcaacgcg tgccaccatt tccatctcga
    42121 gatcagtacc ggtcagcgac aacgtaccgt cagcaacctg taacagcaga ttaccgagaa
    42181 tcggtagcgt aggacgacca cctaacggac cgctcacctg ttgtagcggt tttaataaat
    42241 gctcacgttc tacggtaaat ttcataggtt tacgatgaca atgttctgat taaatttgaa
    42301 aaatcttctt tgatatcgtg gctctcttca cgcaactgct cgatcttacg gcaggcatga
    42361 agcaccgtcg tgtggtcacg cccaccaaac gcatcgccaa tctctggcag actgtggtta
    42421 gtcagctctt tcgctagcgc catcgccatc tggcgcggac gcgccaccga gcgggatcga
    42481 cgcttggaaa ggagatccgc gactttgatc ttgtagtact ccgccaccgt cttctgaata
    42541 ttgtcgatgg tgaccagttt ttcctgcaat gccagcaagt cgcgcagcgc ctcacgcacg
    42601 aagtcgatgg tgatcgcccg tccggtaaag ttggcgttag caataacgcg gttcagcgcc
    42661 ccttccagct cacgtacgtt agatcgtaga cgcttggcga taaagaacgc cacttcaccc
    42721 ggcaaacgaa tgtcattttc gtcggccttt ttcatcagga tcgccacacg ggtttccagc
    42781 tctggcggtt cgatcgccac agtcagtccc caaccgaagc gggatttcaa acgatcctca
    42841 acgccgttga tctctttcgg atagcgatcc gaggtgagaa tgatctgttg attaccttcc
    42901 agcagggcgt tgaaggtgtg gaaaaactct tcctgagatc gttctttatt agcaaaaaac
    42961 tgaatatcgt cgatcagcag tgcatctacg gaacggtagt agcgtttaaa ctcttcgatc
    43021 gcgttgtttt gcagggcttt aaccatgtcc tgaacaaagc gctcggagtg catataaacc
    43081 actttggcat tcggcttgcg cgccataatg ccgttaccca ccgcatgcag caggtgagtt
    43141 ttacccagac ccgtgccgcc ataaaggaac aacgggttat aggcaccgcc agggttatcc
    43201 gccacctggc aagccgccgc gcgcgccagt tggttagatt taccttcaac gaagttatca
    43261 aacgtgtgtt tgacgtttac gttagaacga taggtcggtt ctgccggggc cgggacgtta
    43321 tcccaacctg agcgcgtaga aggcgcagca cgttgcggct gcgtttgcgc cacctgtgca
    43381 ggggccgcga cgttgctcgt cactgccgct tgtggcgttt gcgtcaccgg tttggtgccg
    43441 acttcaaaac gcagctgtgg ggcatccgct ccgcagaaac tggttagcag tccattgata
    43501 ttattaaggt acttgtcccg tacccaatcg aggacaaaac ggtttggcgc gtacagggcc
    43561 agcgtgttat cgctcagttc cgcctgcaat gggcgtatcc acatactgaa ttctgtggct
    43621 ggtaactcat cctgcaatcg ggcaagacac tgctgccaaa gcgaaagtga cacggcggac
    43681 tccactcgaa caaaagtcga taatgactaa ggctgaaaca ttcatgattg ttgacgtacg
    43741 tcgaaaagac cctgcttgag ggtgacgcac gaaccgctgt ctgcggttat atgcccgatc
    43801 aagatcctgc aaaacgatcg ggaccgcgga tcatagccta aactgcgcaa gagatcttct
    43861 gtttctcaca gattttccct atttatccac aggactttcc agaactcgct aagtgtaaac
    43921 gatcctgccg cgaggcgggc acgatttacg ccgcatattg gaaaatttaa tgaccataga
    43981 caaaaattgg cttaatcgat ctaataaaga tccaggacga tccttgcgct ttacccatca
    44041 gcccgtataa tcctccaccc ggcgcgccat gctggtttcc actggtgtga ggtcgtacat
    44101 tttccctgcg aaaaggtgcg gaaaagcgcg gtaaataagg aaagagaatt gactccggtg
    44161 tgtacaatta ttacaatccg gcctctttaa tcacccatgg cttcggtgtc catcgtttca
    44221 tttttcggcg gatatccaat aaagccattg aatttattca agtttaggta gaaatcgcca
    44281 tgaaacgcac ttttcaaccg tctgtactga agcgcaaccg ttctcacggc ttccgtgctc
    44341 gtatggctac taaaaatggt cgtcaggttc tggcacgtcg tcgtgctaaa ggccgcgctc
    44401 gtctgaccgt ttctaagtaa taaagctaac ccctgagtgg ttaagctcgc atttcccagg
    44461 gagttacgct tgttaactcc cagtcaattc acattcgtct tccagcagcc acaacgggct
    44521 ggcacgccgc aaattaccat tctcggccgc ctgaattcgc tggggcatcc ccgtatcggt
    44581 cttacagtcg ccaagaaaaa cgttcgacgc gcccatgaac gcaatcggat taaacgcctg
    44641 acgcgtgaaa gcttccgtct gcgccaacat gaactcccgg ctatggattt cgtggtggtg
    44701 gcgaaaaaag gggttgccga cctcgataac cgtgctctct cggaagcgtt ggaaaaatta
    44761 tggcgccgcc actgtcgcct ggctcgcggg tcctgatagc cctcattcgg gtctatcaac
    44821 gcctgattag tccgctactc gggccgcatt gtcgtttcac tccaacctgt tcaagctacg
    44881 gaattgaggc attgcgcagg tttggagtga taaaaggcag ttggttgacg gtgaaacgcg
    44941 tattaaaatg ccacccttta caccctggtg gtgacgatcc cgtcccgccc ggaccatttg
    45001 ataccagaga acactaacga tggattcgca acgcaatctt ttagtcatcg ctttgctgtt
    45061 cgtgtctttc atgatctggc aagcctggga gcaggataaa aacccgcaac ctcaggccca
    45121 acagaccacg cagacaacga ccaccgcagc gggtagcgcc gccgaccagg gcgtaccggc
    45181 cagtggccag gggaaactga tctcggttaa gaccgacgtg cttgatctga ccatcaacac
    45241 ccgtggtggt gatgttgagc aagctctgct gcctgcttac ccgaaagagc tgaactctac
    45301 ccagccgttc cagctgttgg aaacttcacc gcagtttatt tatcaggcac agagcggtct
    45361 gaccggtcgt gatggcccgg ataacccggc taacggcccg cgtccgctgt ataacgttga
    45421 aaaagacgct tatgtgctgg ctgaaggtca aaacgaactg caggtgccga tgacgtatac
    45481 cgacgcggca ggcaacacgt ttaccaaaac gtttgtcctg aaacgtggtg attacgctgt
    45541 caacgtcaac tacaacgtgc agaacgctgg cgagaaaccg ctggaaatct cctcgtttgg
    45601 tcagttgaag caatccatca ctctgccacc gcatctcgat accggaagca gcaacttcgc
    45661 actgcacacc ttccgtggcg cggcgtactc cacgcctgac gagaagtatg agaaatacaa
    45721 gttcgatacc attgccgata acgaaaacct gaacatctct tcgaaaggtg gttgggtggc
    45781 gatgctgcaa cagtatttcg cgacggcgtg gatcccgcat aacgacggta ccaacaactt
    45841 ctataccgct aatctgggta acggcatcgt cgctatcggc tataaatctc agccggtact
    45901 ggttcagcct ggtcagactg gcgcgatgaa cagcaccctg tgggttggcc cggaaatcca
    45961 ggacaaaatg gcagctgttg ctccgcacct ggatctgacc gttgattacg gttggttgtg
    46021 gttcatctct cagccgctgt tcaaactgct gaaatggatc catagctttg tgggtaactg
    46081 gggcttctcc attatcatca tcacctttat cgttcgtggc atcatgtacc cgctgaccaa
    46141 agcgcagtac acctccatgg cgaagatgcg tatgctgcag ccgaagattc aggcaatgcg
    46201 tgagcgtctg ggcgatgaca aacagcgtat cagtcaggaa atgatggcgc tgtacaaagc
    46261 tgagaaggtt aacccgctgg gcggctgctt cccgctgctg atccagatgc caatcttcct
    46321 ggcgttgtac tacatgctga tgggttccgt tgaactgcgt caggcaccgt ttgcactgtg
    46381 gatccacgac ctgtcggcac aggacccgta ctacatcctg ccgatcctga tgggcgtaac
    46441 gatgttcttc attcagaaga tgtcgccgac cactgtgacc gacccgatgc agcagaagat
    46501 catgaccttt atgccggtca tcttcaccgt gttcttcctg tggttcccgt caggtctggt
    46561 actgtactat atcgtcagca acctggtaac cattattcag cagcagctga tttaccgtgg
    46621 tctggaaaaa cgtggcctgc atagccgcga gaagaaaaaa tcctgattcg gtgagttttc
    46681 gctaaaataa gggcggtcag ttgaccgcct tttttctttt cgtagggcgg ataagcaccg
    46741 cgcatccgcc acacaaagca acaggaacat catgagcgat aatgacacta tcgtagccca
    46801 ggccacgcct ccgggacgtg gcggcgttgg catcctgcgc atctccggct tcaaagcccg
    46861 tgaagttgcc gaaaccgtgc tgggtaaact gcctaagccg cgctacgccg attatcttcc
    46921 gtttaaagac gccgacggca gcgtgctcga tcaggggatt gcgctatggt tccctggccc
    46981 gaactcgttc accggcgaag atgtgctgga actgcaaggt catggcggtc cggtgatcct
    47041 cgacctgctg ttaaaacgca ttctgaccat tcccggcctg cggattgctc gccctggtga
    47101 gttttccgaa cgcgcgtttc ttaacgataa acttgactta gcccaggccg aggcgattgc
    47161 cgatcttatc gacgccagtt cggaacaggc ggcccgttcg gcacttaact cgctgcaagg
    47221 cgcattctcc gcacgggtta atcatctggt agaagccctc acccacttgc gcatttacgt
    47281 cgaagcggca attgatttcc ctgatgaaga gatcgatttc ctctccgacg gcaaaattga
    47341 agcccagctt aatgacgtga tggccgatct cgatgcagtg cgtgctgaag cacgtcaggg
    47401 tagtttgttg cgcgaaggga tgaaagtagt gattgccgga cgtcctaatg ccggtaaatc
    47461 gagcctgtta aacgcgctgg cgggccgtga agcggcaatc gtaaccgata tcgccggaac
    47521 cacgcgtgac gtgctgcgtg agcatatcca cattgacgga atgccgctgc atatcatcga
    47581 cactgccggg ctacgtgagg ccagtgacga agtggaacgt attggtatcg agcgcgcgtg
    47641 gcaggaaatt gaacaggccg accgcgtgct gtttatggtc gatggcacca cgacagacgc
    47701 cgttgatccg gcagagatct ggccggaatt tatcgcccgt ctgcccgcga aactgccgat
    47761 caccgtggta cgcaataaag ccgatatcac cggcgaaacg ctgggaatga gtgaagtgaa
    47821 cggtcacgcg ttaattcgtc tctcggcaag gactggtgaa ggcgtggacg tgctgcgtaa
    47881 ccatctcaaa cagagcatgg gctttgacac caatatggaa ggcggcttcc tggcgcgccg
    47941 tcgccactta caggcgctgg aacaggcagc ggagcatttg cagcaaggta aagcgcaact
    48001 gttgggagcc tgggcaggtg aactgctggc ggaagagttg cgcctggcac agcagaactt
    48061 aagcgaaatc accggggaat ttacttcaga cgacctgctg gggcggattt tctccagctt
    48121 ctgtattggt aagtaaccgc gcttacgaag ccgtattctg actgtcagat gcggcttcgc
    48181 ttcattgtta gcactcctgt tatgcctcaa ccctttttta aacattaaaa ttcttacgta
    48241 atttataatc tttaaaaaaa gcatttaata ctgctccccg aacgattgtg attcgattca
    48301 catttaaaca atttcagaat agacaaaaa
//
LOCUS       DAJTRD010000023        47955 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTRD010000023 DAJTRD010000000
VERSION     DAJTRD010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47955
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.023"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO315_001426"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO315_001426"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5890393.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO315_001427"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO315_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5890394.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO315_001428"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO315_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5890395.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO315_001429"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO315_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5890396.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO315_001430"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO315_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5890397.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO315_001431"
     CDS             5377..5835
                     /locus_tag="OO315_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5890398.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO315_001432"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO315_001432"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5890399.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO315_001433"
     tRNA            6887..6971
                     /locus_tag="OO315_001433"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO315_001434"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO315_001434"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO315_001435"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO315_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5890400.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO315_001436"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO315_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5890401.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO315_001437"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO315_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5890402.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO315_001438"
     CDS             complement(10252..13995)
                     /locus_tag="OO315_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5890403.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO315_001439"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO315_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5890404.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO315_001440"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO315_001440"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5890405.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO315_001441"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO315_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5890406.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO315_001442"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO315_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5890407.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO315_001443"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO315_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5890408.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO315_001444"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO315_001444"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5890409.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO315_001445"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO315_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5890410.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO315_001446"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO315_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5890411.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO315_001447"
     CDS             23392..23499
                     /locus_tag="OO315_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5890412.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO315_001448"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO315_001448"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5890413.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO315_001449"
     CDS             complement(24538..25347)
                     /locus_tag="OO315_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5890414.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO315_001450"
     CDS             complement(25351..25743)
                     /locus_tag="OO315_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5890415.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO315_001451"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO315_001451"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5890416.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO315_001452"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO315_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5890417.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO315_001453"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO315_001453"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5890418.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO315_001454"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO315_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5890419.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO315_001455"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO315_001455"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5890420.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO315_001456"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO315_001456"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5890421.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO315_001457"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO315_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5890422.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO315_001458"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO315_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5890423.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO315_001459"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO315_001459"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5890424.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO315_001460"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO315_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5890425.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="OO315_001461"
     CDS             36790..39657
                     /locus_tag="OO315_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5890426.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO315_001462"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO315_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5890427.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="OO315_001463"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="OO315_001463"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5890428.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="OO315_001464"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="OO315_001464"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5890429.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="OO315_001465"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="OO315_001465"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5890430.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="OO315_001466"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="OO315_001466"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5890431.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYRDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47581)
                     /locus_tag="OO315_001467"
                     /pseudo
     CDS             complement(47142..47581)
                     /locus_tag="OO315_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        12042 a        12343 c        11741 g        11829 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaaa ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcattac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct accgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattctaaat gttcaataag cgcagccaaa accgcctcac
    47521 tatccgcccc atttttaacg cctgactggc tactgtctaa aatatttatt ttctgaatca
    47581 caccaaaaac aacgaaacaa taattaaaaa aggattctgt tgttgttacc gggaaccgac
    47641 ttatttcgct cagaaagtcg ttcaaccagc tccgcagtga taagattcgc tgatgataca
    47701 gtgtatcctc actgcgtgct ggaggtgcca gtataatatg ttgatcaata cagtatttat
    47761 tgtgttcagt cgtttcctga tgcgtttggt tctctgcgga ttgtcgctgg cgaaataaat
    47821 ggcttgcggt aaatgccgtt acagagcagc gcaacgcgtc aattccttcg gagtgcccga
    47881 cttttggcga gagttccggt tttatgcgtc caggtagcgg aaatttcacc cgaatgagaa
    47941 atgcattaat aaaat
//
LOCUS       DAJTRD010000024        47363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTRD010000024 DAJTRD010000000
VERSION     DAJTRD010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.024"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            18..500
                     /gene="sseA"
                     /locus_tag="OO315_001468"
                     /pseudo
     CDS             18..500
                     /gene="sseA"
                     /locus_tag="OO315_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
     gene            complement(1319..2095)
                     /gene="sseB"
                     /locus_tag="OO315_001469"
     CDS             complement(1319..2095)
                     /gene="sseB"
                     /locus_tag="OO315_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5890432.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2237..3520)
                     /gene="pepB"
                     /locus_tag="OO315_001470"
     CDS             complement(2237..3520)
                     /gene="pepB"
                     /locus_tag="OO315_001470"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5890433.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3579..3779)
                     /gene="iscX"
                     /locus_tag="OO315_001471"
     CDS             complement(3579..3779)
                     /gene="iscX"
                     /locus_tag="OO315_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5890434.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3791..4126)
                     /gene="fdx"
                     /locus_tag="OO315_001472"
     CDS             complement(3791..4126)
                     /gene="fdx"
                     /locus_tag="OO315_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5890435.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4128..5978)
                     /gene="hscA"
                     /locus_tag="OO315_001473"
     CDS             complement(4128..5978)
                     /gene="hscA"
                     /locus_tag="OO315_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5890436.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5995..6510)
                     /gene="hscB"
                     /locus_tag="OO315_001474"
     CDS             complement(5995..6510)
                     /gene="hscB"
                     /locus_tag="OO315_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5890437.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6606..6929)
                     /gene="iscA"
                     /locus_tag="OO315_001475"
     CDS             complement(6606..6929)
                     /gene="iscA"
                     /locus_tag="OO315_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5890438.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6946..7332)
                     /gene="iscU"
                     /locus_tag="OO315_001476"
     CDS             complement(6946..7332)
                     /gene="iscU"
                     /locus_tag="OO315_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5890439.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7360..8574)
                     /gene="iscS"
                     /locus_tag="OO315_001477"
     CDS             complement(7360..8574)
                     /gene="iscS"
                     /locus_tag="OO315_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5890440.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8686..9174)
                     /gene="iscR"
                     /locus_tag="OO315_001478"
     CDS             complement(8686..9174)
                     /gene="iscR"
                     /locus_tag="OO315_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5890441.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9444..10184)
                     /gene="trmJ"
                     /locus_tag="OO315_001479"
     CDS             complement(9444..10184)
                     /gene="trmJ"
                     /locus_tag="OO315_001479"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5890442.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10303..11106
                     /gene="suhB"
                     /locus_tag="OO315_001480"
     CDS             10303..11106
                     /gene="suhB"
                     /locus_tag="OO315_001480"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5890443.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11251..12105
                     /locus_tag="OO315_001481"
     CDS             11251..12105
                     /locus_tag="OO315_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5890444.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12296..13576
                     /gene="csiE"
                     /locus_tag="OO315_001482"
     CDS             12296..13576
                     /gene="csiE"
                     /locus_tag="OO315_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5890445.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13568..14707)
                     /locus_tag="OO315_001483"
     CDS             complement(13568..14707)
                     /locus_tag="OO315_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5890446.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14867..15757)
                     /gene="hcaR"
                     /locus_tag="OO315_001484"
     CDS             complement(14867..15757)
                     /gene="hcaR"
                     /locus_tag="OO315_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5890447.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15893..17254
                     /locus_tag="OO315_001485"
     CDS             15893..17254
                     /locus_tag="OO315_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5890448.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17251..17769
                     /locus_tag="OO315_001486"
     CDS             17251..17769
                     /locus_tag="OO315_001486"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5890449.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17769..18089
                     /locus_tag="OO315_001487"
     CDS             17769..18089
                     /locus_tag="OO315_001487"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5890450.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18086..18898
                     /gene="hcaB"
                     /locus_tag="OO315_001488"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18086..18898
                     /gene="hcaB"
                     /locus_tag="OO315_001488"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5890451.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETPIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18908..20135
                     /locus_tag="OO315_001489"
                     /pseudo
     CDS             18908..20135
                     /locus_tag="OO315_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            20232..20654
                     /locus_tag="OO315_001490"
     CDS             20232..20654
                     /locus_tag="OO315_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5890452.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20702..21574)
                     /locus_tag="OO315_001491"
     CDS             complement(20702..21574)
                     /locus_tag="OO315_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5890453.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21586..22680)
                     /locus_tag="OO315_001492"
     CDS             complement(21586..22680)
                     /locus_tag="OO315_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5890454.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22713..23711)
                     /locus_tag="OO315_001493"
     CDS             complement(22713..23711)
                     /locus_tag="OO315_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5890455.1"
                     /translation="MYASSLLLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23736..25247)
                     /locus_tag="OO315_001494"
     CDS             complement(23736..25247)
                     /locus_tag="OO315_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5890456.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25263..26257)
                     /locus_tag="OO315_001495"
                     /pseudo
     CDS             complement(25263..26257)
                     /locus_tag="OO315_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26354..29455)
                     /locus_tag="OO315_001496"
     CDS             complement(26354..29455)
                     /locus_tag="OO315_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5890457.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29462..29635)
                     /locus_tag="OO315_001497"
     CDS             complement(29462..29635)
                     /locus_tag="OO315_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890458.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29753..30946
                     /locus_tag="OO315_001498"
     CDS             29753..30946
                     /locus_tag="OO315_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5890459.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31144..32397)
                     /gene="glyA"
                     /locus_tag="OO315_001499"
     CDS             complement(31144..32397)
                     /gene="glyA"
                     /locus_tag="OO315_001499"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5890460.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32724..33914
                     /gene="hmpA"
                     /locus_tag="OO315_001500"
     CDS             32724..33914
                     /gene="hmpA"
                     /locus_tag="OO315_001500"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5890461.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33959..34297)
                     /gene="glnB"
                     /locus_tag="OO315_001501"
     CDS             complement(33959..34297)
                     /gene="glnB"
                     /locus_tag="OO315_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5890462.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34358..35692)
                     /gene="glrR"
                     /locus_tag="OO315_001502"
     CDS             complement(34358..35692)
                     /gene="glrR"
                     /locus_tag="OO315_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5890463.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35682..36395)
                     /gene="qseG"
                     /locus_tag="OO315_001503"
     CDS             complement(35682..36395)
                     /gene="qseG"
                     /locus_tag="OO315_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5890464.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36560..37987)
                     /gene="qseE"
                     /locus_tag="OO315_001504"
     CDS             complement(36560..37987)
                     /gene="qseE"
                     /locus_tag="OO315_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5890465.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38545..42432)
                     /gene="purL"
                     /locus_tag="OO315_001505"
                     /gene_synonym="purI"
     CDS             complement(38545..42432)
                     /gene="purL"
                     /locus_tag="OO315_001505"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5890466.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42691..44247
                     /gene="mltF"
                     /locus_tag="OO315_001506"
     CDS             42691..44247
                     /gene="mltF"
                     /locus_tag="OO315_001506"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5890467.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44244..44780)
                     /gene="tadA"
                     /locus_tag="OO315_001507"
     CDS             complement(44244..44780)
                     /gene="tadA"
                     /locus_tag="OO315_001507"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5890468.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44805..45440)
                     /gene="yfhb"
                     /locus_tag="OO315_001508"
     CDS             complement(44805..45440)
                     /gene="yfhb"
                     /locus_tag="OO315_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5890469.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45649..46497
                     /locus_tag="OO315_001509"
     CDS             45649..46497
                     /locus_tag="OO315_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5890470.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46836..47363)
                     /locus_tag="OO315_001510"
                     /pseudo
     CDS             complement(46836..47363)
                     /locus_tag="OO315_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11296 a        12974 c        11913 g        11180 t
ORIGIN      
        1 tacgaataaa gtaacgggga cttgcaggct ggcagcgcga tgacctgcta ttagaagaag
       61 gtgcagtaga gctgccggaa ggagagttta acgccgcgtt taatcctgaa gcagtggtga
      121 aagtaaccga tgtgttattg gcaagccatg aaaatacggc gcaaattatt gatgcccgcc
      181 cggctgcacg ttttaacgca gaagttgatg aacctcgccc aggtttacgt cgcggacata
      241 ttcccggtgc attgaacgtt ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga
      301 ccgatgaact ggatgcgata ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg
      361 tcagctgcgg ctctggtgta acggcagccg tggttttgtt agcactcgcg acgctggatg
      421 tgccaaacgt gaaactgtac gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac
      481 cggttgagcc agtgaaataa gtatattaca ggcaataaaa aaccgccgaa tttggcgttt
      541 ttttattgct ggtctggttc gcggcctttc cagcaggttg acttgtgtta catgagcaac
      601 gcaggtgctt cacagcaaaa caatactcac cagtaactct ctttttgtca agcaaaagag
      661 agtaattatt gtttatttag cgtattatcg acaccggccc tttccgccgt gttcggtaat
      721 aaaataacct ggcttattag tccgaattca gacaaatata aacaaatcct gctcaaaatt
      781 aaaaattcta accggtaaaa gatattactt aaacatgtaa atttactttc ctttaaaaaa
      841 caaaaaaccg ccaaaatcag gcggttattt gttgctggtc cggttcgcgg cctttccagc
      901 aggttgtatt accgtagtaa tgcaagcgcg tctcagcgga gacaatactc gccagtaact
      961 ctctttttgt caagcaaaag agagttatta ttgttctgtt agtgtattat ccactgcggc
     1021 cctttccgcc gtctcgcaag cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag
     1081 agcaagcgcg tctcagcgct tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc
     1141 ggtcttcgca tggaccgcac aatgaagata cggtgcttct gtatcgtact tattgtttct
     1201 ggtgcgctgt tttcccaagt aaataataac cggagtctct ccggcgacaa tttactggtg
     1261 gttaacaacc ttcagagcag caagtaagcc cgaatgccgc cctttagggc ggcatatttt
     1321 agattatccg gttttgttta aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg
     1381 gcgcaatatg ttcggtaata aagtggctaa ttcctttttc cccttttttc acctgacaaa
     1441 tatcgattgg ttcatcgcca ggtaatgtat cggtcgctac acttcccgtc gcctgaataa
     1501 tttcttcgat atcaccatcg gcttcaatgc caataagtaa attaggctgt gcctcttcgt
     1561 tctctttaat tgaacaaata aaagcacgct tcaccggctt aatggtttta aataaggtgg
     1621 tgagtgaatc aatcatttgt gctggcggct ctgcgacttc cgataatatc agcgattcac
     1681 cgccttccag gatttcctgg ctgctcagcg gatttccctc ttcaccaatc aacaaactga
     1741 tttcacgcgg cataaattct ttaccggtcg gcagtttggc attaaggaag agcgtttcgc
     1801 caagggtcat ctcaaacaat gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa
     1861 ccgcctgttg aagcgcttct aacgaggtga aaaaaggaat gacgctggtg ccgtcttctt
     1921 tttcccagtg ctgtaaatca agcgcactat cttcaaccac agcctcgccc tgcgccgccg
     1981 taccaggcac ccagacggtg gattccagta gagtacggaa aaaggccggg cggtgcgccg
     2041 gttcggttgc tgctttttcc agcaggtctt caagttcgtt ttttgtttcg gacataagaa
     2101 ccacaattca ttcaacgttc ggggcgcaaa tgcctgatgc gctacgctta tcaggcctac
     2161 aagggattcg caatttgttg aatttgcaga atttgtaggc cggataaggc gtttacgccg
     2221 catccggcat attagtttac gccgttaaca gattagctat cgtgcgcaca ccaagtcccg
     2281 tagcgcccgc agaccactgt tcaaccggcg ctttacggta agtcgccgag cagtcgatat
     2341 gcagccagcc ttgctgatag ttctcaacaa agtgcgacag gaagcccgcc gccgtgctcg
     2401 cgcctgccgg atacgccgcg cttccggtat tgttcagttc ggcaaagtta gacggcagct
     2461 ggctgcggtg gaattccgcc agcggcagac gccagaatgg ttcgttctct tgtgaggcac
     2521 tcgccagtaa acgaccggca agcgcatcgt caaaactgaa cagcgcgtga taatcattac
     2581 ccagcgcagt tttcgccgcc ccggtgaggg tcgccgcatc aatgatcatt tccggtttct
     2641 gcgcactggc atcaatcaga ccatcggcaa gcaccagacg cccttcggca tcagtgttca
     2701 tcacttcaac ttttttaccg ttgcgatagg tgatgatatc gcccagcttg aacgcattgc
     2761 cgctaatcag gttatccgca cagcagagga acagcttcac gcgcttgttc agtccgcgca
     2821 taatggcaaa tgccagcgcc ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg
     2881 agtccataaa cgcagtctgt ttgatgctgt agccgccgga gtcaaaagtg atacctttac
     2941 ctaccaggca cgcgtacacc ggtgcttctt tatcgccagt tgggttgtaa tccagcgcca
     3001 gcaataccgg agaacgttct gaaccgcgcc cgacagtgtg cagccccata taaccttgct
     3061 cacgcagatc ttcgcctttg gtgatccgat aagtcacacg atcgcccgcg acgttgctga
     3121 tcagatcaac agcacgctgt gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga
     3181 tggtgtcacg cacccagtcg atgatcatca ggcggttatc cagttcctgg cgctgggcat
     3241 cgtccagatc cggccactcc actttacgcg tgcctttcgg gcctttataa ccttgccaga
     3301 atgcccagca gcgatccgca tcccagcctt caccgcttaa ctgaacatgc ttgatgccca
     3361 gaccgtcaat cttgcgggcc gcacgctgga tcagccccag atcgtctgcc ccgttcaaat
     3421 gcagggtaat gccgtcatta ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg
     3481 caggttgggt agagagggta atcttcatcg cttctgtcat tttagttatc cttattcgtt
     3541 taacagcaaa acgggctacc cccaggcagc ccgttcattc actcggcttc gtccagccag
     3601 actaacaaaa tcgcttcgag gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct
     3661 tccagatcgc aaatccactg atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg
     3721 ggatacgcat cgtacagtgc ttcgccaatt tcgcggctat cggtccactt aagtcccata
     3781 ctaacctctg ttaatgctca cgcgcatggt tgatagtgta acgcgggatt tcgaccacta
     3841 aatcttcgtc ggtgacgcgc gcctggcagc ttaaacggct ttccggctcc agtccccagg
     3901 ctttgtccag catgtcgtct tcctgctctg agctttccgg cagtgagtca aaaccttcac
     3961 gaacgatgca gtggcaggtg gtgcaagcac aggatttttc acaggcgtgt tcaatctcaa
     4021 taccgttacg cagcgctgcg tcgagaatgg tttcaccgct attagcttcc agaacagcgc
     4081 catcagggca gaggtcctga tgaggcaaaa taacaatctt tggcatatta aacctcgtcc
     4141 acggaatggc ctttcagcgc acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc
     4201 tgggtttgtt tgtctacgtt tttaatcgct tgttcgatgg cgtcaacatc atcgccctgc
     4261 gccacttcac tcaggtgagc ggcagcattg tcaatgacct gacgctctgc ggcgcttaac
     4321 agcgcggcat cagcagccag cgcgccgtgc agactttcca gcacacgcgc cgcttctact
     4381 ttttgttctg ccagcatacg ggcttttacg tcctgctcgg catagctcat tgagtctttg
     4441 atcatcgaag cgatttcgct atcggtcaga ccgtaagacg gtttgacctg aatagacgcc
     4501 tcaacgccgg tggatttctc catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg
     4561 aacgtcacgc gaatatgcgc accgccagcc ggtagcgccg gaataccacg cagcgcaaaa
     4621 cgcgccagtg agcggcagtc ctgcaccagt tcgcgctcac cctgcattac atggatagac
     4681 atcgccgtct gaccatcttt aaaggtggtg aaatcctgag cgcgggccac cggaatagtg
     4741 gtattacgcg gaatcacttt ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt
     4801 gggatcacat caagcagcag catttcgctg tctggcttgt tacccaccag aatatccgcc
     4861 tgaatcgccg cgccaatagc gacgacttta tccgggtcga tggaagtcag cggtggacga
     4921 ccgaaaaatt cgcctacccg ttcacgcacc agcggcacgc gagtagaacc gcccaccatc
     4981 accacttcca gcacttcgtc agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc
     5041 agtaaggttc gttttaccag tggcgcgatc agttcattga attgttcacg gctgatttcg
     5101 ccctgccagc ccgcaacgtt aacggtcacg gagtccgcat cgctcagcgc gattttggct
     5161 gcaatggtgg catccagcag ttcacgctga acgcggttat cgctacgatc aggaatgtcc
     5221 gcctgctcgc gaatgtaatc cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa
     5281 tcaccgccgg ttgccagcac ttcaaacacg ccgcgactta agcgcagaat ggaaatatca
     5341 aacgtcccgc caccgaggtc ataaacagcg atcacgcctt cctgaccgga atccagcccg
     5401 taggcgatag ccgcagcagt cggttcgtta agtaagcgca gaacatgcaa ccccgccaga
     5461 cgcgccgcgt ctttggtgcc ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata
     5521 actacaccat ccagctcgcc tgccagggct tcagttgccc gcgccgccag tgctttgagg
     5581 atgtccgcag aaacgcgcac cgggttcagt agccccgccg ccgtttcaat catcggcagg
     5641 ccgttttcgc tggcctggaa ttgataaggc agatgcggat agcgttgctg gatatcagcc
     5701 agcgagcgcc ccatcaggcg tttaacagaa ctaatggtgt tggcggtgtc aagcgctgcg
     5761 ttagtacgcg cgtcataacc caccgaatgc ccttgctgtt gatagtgaac aacagatggc
     5821 agcaggtgac ggccttcatg gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc
     5881 accagcgagt tggttgtgcc caggtcaata ccagccgcca gacgacgctg atgcggcgca
     5941 gcactcaaac caggttcact aatttgtaat aaggccatgt ttagcttcca gaaattaaaa
     6001 atcgagcagt ttttcttcga gttgttcggc actgctccgc agtttatcga gaaaacgcag
     6061 cttacgcacc gtatccgccg ccgcgtccca cgcctcgttg tctaactgtt caaccatcaa
     6121 ctgatggcgg gtatcaaaca tctttttcac acgtttgata aagctttcca gccgcgcttc
     6181 atctttcgcc tgttcgatct cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa
     6241 cgcggtgtcg cgcacagtat gctgctcgct ggcgagatca aaaccgtgta aagaaagcaa
     6301 atattccgcg cgcattaacg gatgacgcag cgtttgccag gcctggttaa tggttgcaga
     6361 ttgctgtacg gcggcgagtt gttccgccag gcttccgctg gcgaatttat caggatgata
     6421 ctgacgttgt agatcctgaa aacgcaggct cagtgcctgg gtatcgagtt gataacgggc
     6481 aggcaagcca aagagggtga agtaatccat aacattctca gggctgcgat ccgccgcgat
     6541 aacggcgaat caggtaaaac aaaccccacg cgcaggcgac cacggtgggg ttatcggtat
     6601 gcgcatcaaa cgtggaagct ttcgccgcaa ccacactcat ctttaacgtt cgggttggtg
     6661 aatttaaacc cttcattcag gccttctttt acgaagtcca gctgcgtacc gtccagaaat
     6721 tgcaggcttt tgccatcgac cacgactttc acgcctttgt cttcaaacac gatgtcttcc
     6781 ggcgtcggtt cgtcaacaaa ttccagtaca taagccatac ctgaacatcc ggaggttctc
     6841 acgcccagac gcaggccaaa ccctttgccg cggttagcca gaaaggtatt tactcgcgct
     6901 gctgcactgt cgctcagtgt aatcgacata accaaacctc aactcttatt ttgcttcacg
     6961 tttgctttta tagtccgcaa tggcggcttt gatcgcgtct tctgccagaa tagaacagtg
     7021 aattttcacc ggcggcagtt caagttcttc agcaatatcg gtgtttttga tcgcctgcgc
     7081 ttcgtcgaga gacttccctt tcacccattc ggtgaccagg gagctggaag cgatagcgga
     7141 accgcagccg taagttttaa aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat
     7201 ctgcaacttc attacgtcgc cacaggccgg tgcacccacc atgccgctgc cgacgttctc
     7261 gtcgttgtta tcaaaggaac ccacgttacg cggattctcg taatggtcga taactttttc
     7321 gctgtaagcc attataaatt ctcctgattc cgataccgat taatgatgag cccattcgat
     7381 gctgttcaga tccacgccct gcttgtacat ttcccacagc ggagaaaggt cacgcagacg
     7441 accgatggat ttacgaacta actcgatggt gtagtcgatc tcttcttcag tagtaaaacg
     7501 acctaaagag aaacggatag agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag
     7561 cacgtaggac ggttcgaggc ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc
     7621 tttcagcgcc ataatcagcg actcaccttc aacgtagttg aagctgacgt tgagaatgtt
     7681 cggcgcaccg tgttccaggt caccgttcag gtaaacttct tcgatatctt tgatgccgtt
     7741 ccacagacgg ttacgcaggc cgcgcagacg ttccatctcg gtcgccatct cttcttttgc
     7801 gatgcgatag gcttcgccca ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat
     7861 accgcgctcg tgaccgccgc cgtgcatttg cgcttcgatg cgtacacgcg gtttacgacg
     7921 cacatacagc gcaccgatac ctttcgggcc atagattttg tgaccggaga aagacatcag
     7981 gtcaactttc aactggctca ggtcgattgg cagtttgccc acgctctggg ttgcatcaac
     8041 gtgatagata atgccacgag cacggcacat ttcgccgata gccgcgatat cctgcaccac
     8101 gccgatttcg ttatttacgt gcatgatgga aacaaggatg gtgtcgtcac gcatcgccgc
     8161 ttcgagttct ttcaggtcga taataccgtt acgctgcggt gccaggtagg tgacttcgaa
     8221 accttcgcgc tccagctgac ggcaagtatc cagtaccgct ttgtgttcgg ttttgctggt
     8281 gatgatgtgc ttgccttttt tctgataaaa gttggctgca cctttgatcg ccaggttgtc
     8341 agattcggtt gcaccagagg taaagacgat ttcacgcgga tcagcgccga ccagatcggc
     8401 aatctgatta cgggcgatat ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg
     8461 agaggccggg ttaccaaagg ttccgtccat cgtcatgaac tgcatcattt tctcggcaac
     8521 acgcgggtcc accggcgtgg ttgcggagta gtcgagataa atcggtaatt tcattgctct
     8581 ataaactccg tacatcactc aatgcaagga atcaggctac cggctggatg tacgaccgtg
     8641 tttacggagt atttagcact ccggcctgat tctgaattct ttttattaag cgcgtaactt
     8701 aacgtcgatc gcgtcttgtg tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga
     8761 cacatccagc acttcctggt tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc
     8821 ggtgagacgg tcgctcaaat cacgccacag cgcgtgggtc aggcatttat cgccgccctg
     8881 gcagccgcct ttaccctgac aacgggtggc atctacagat tcgtcaacgg cgctaatcac
     8941 ttcgccaacg gcgatgctgc tggcatcttt gcctaacaga taaccaccgc ctggtccacg
     9001 tacgctggaa accagaccat ttttacgcag acgggaaaac agttgttcca gataagaaag
     9061 ggaaattccc tgacgttcgg aaatatcagc caacggtacc gggcccgctt cagagttgag
     9121 cgcaacgtca agcattgcgg tcacggcata gcgcccttta gatgtcagtc tcatgtctta
     9181 cttcacctca aactcgcccc tgcccggggt tttttattgt aaaagtgggg gtattgcata
     9241 gcagggtcaa gtctgacatt cccgagtaaa ttggtcaact atttacttga ctgatttagt
     9301 cgggtattta accttcagtg ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc
     9361 ggtgtgaaca ccttatccga cctacgattc tgtacattgt aggcctgata agacgcgtta
     9421 agcgtcgcat caggcaacca ctgttattcg gccttgttac ctttattctg ctgctcaata
     9481 gaagccagaa tcccgcgcag gatattcaat tcctggcttt ccgggcgcgc acgggtaaac
     9541 agacggcgca atttattcat cacctgcccc ggatggtttt cacggataaa accggttgcc
     9601 agcagcgttt gttccagatg accataaaaa cgctccagat catcgaccag cggatacggc
     9661 gtctcttcat gctcgacctg ctcgccattt tcctgagttg ccagccaggc catacgcact
     9721 tcataggcaa taacctgaac cgccattgcc aggttcagcg aactgtattc cgggtttgcc
     9781 gcaatcgcga catgataatg gcatttctgc aactcttcat tggtcaagcc gacgcgctca
     9841 cgaccaaaca ccagcgccac cggggtattt gccgcttcag cgacgctttt cagaccgcat
     9901 tcgcgcgggt cgagcatcgg ccacggcagc gtgcgggagc gtgcgctggt gcccaccacc
     9961 aggctacaac cggctaacgc ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca
    10021 tcgctggccc ctgcagccag ggcaatcgcc tgggagtcgg gtttcaccag tggattaacc
    10081 agccacagat tggttaatcc cattgttttc atggcacggg caacagaacc catattgccg
    10141 gtgtgtgacg tctccaccag cacaattcga atattttgca gcattgtctt tcttcatcta
    10201 aagattattc acgcatctta tcataaaacg aagacagatg ccgatctcgc tgctatactc
    10261 tgcgccgttt tcccgttctt taacatccag tgagagagac cgatgcatcc gatgctgaac
    10321 atcgccgtgc gcgcagcgcg caaggcgggt aatttaattg ccaaaaacta tgaaaccccg
    10381 gacgctgtag aagcgagcca gaaaggcagt aacgatttcg tgaccaacgt agataaagct
    10441 gccgaagcgg tgattatcga cacgattcgt aaatcttacc cacagcacac catcatcacc
    10501 gaagaaagcg gtgaacttga aggtactgat caggatgttc aatgggttat cgatccactg
    10561 gatggtacta ccaactttat caaacgtctg ccgcacttcg cggtatctat cgccgttcgt
    10621 atcaaaggcc gcaccgaagt tgctgtggta tacgatccta tgcgtaacga actgttcacc
    10681 gccactcgcg gtcaaggcgc acagctgaac ggctaccgtc tgcgcggtag caccgctcgc
    10741 gatctcgacg gtaccattct ggcgaccggc ttcccgttca aagcaaaaca gtacgccact
    10801 acctacatca acatcgtcgg caagctgttc aacgaatgtg cagacttccg tcgtaccggt
    10861 tctgcggcgc tggatctggc ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc
    10921 ggtctgcgtc cgtgggattt cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc
    10981 gtcagcgact tcaccggtgg tcataactat atgctgaccg gtaacatcgt tgctggtaac
    11041 ccgcgtgttg tgaaagccat actggcgaac atgcgtgacg agttaagcga cgctctgaag
    11101 cattaatgac tcaggcgggt gatatcactc acccgccctc gcctttcagg cgctattccg
    11161 aaatacttcc tcaccgcttt actttctttc gtcactctcc caccatcttt ccccgattaa
    11221 tggatgataa agaagtcgca aggaaaggtt atggcactgc cagtgaacaa acgcgttccc
    11281 aaaattctgt ttattctctt tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc
    11341 atcaacttct tctattatcc tgacgataaa atttacggtc ccgacccctg gtcggcggaa
    11401 tccgtcgaat ttacggctaa ggacggtact cgtctgcaag gctggtttat tccttcttcg
    11461 acgggccctg ctgacaacgc catcgcaacc atcattcatg ctcacggcaa tgccggaaat
    11521 atgtccgccc actggccgct ggtcagttgg ttacctgagc gtaatttcaa cgtttttatg
    11581 tttgattatc gcgggtttgg taaatcaaaa ggcactccgt cccaggccgg attgctggac
    11641 gatacgcaaa gcgccatcaa tgtggtgcgc catcgcagtg atgtaaaccc acaacagctg
    11701 gtgctgttcg ggcagagtat tggcggggcg aatattcttg ctgttattgg tcagggtgat
    11761 cgtgaaggca tacgtgcggt gatcctcgac tccacatttg cctcttatgc aaccatcgcc
    11821 aaccagatga tccccggcag tggctactta cttgatgaga gttacagcgg cgaaaattac
    11881 atcgccagcg tcagcccgat cccgctttta ctcattcacg gtaaagccga tcacgttatc
    11941 ccatggcagc acagcgaaaa gttgtatagc ctggcaaaag agccaaaacg gttgatccta
    12001 atcccggatg gcgaacacat tgatgctttt tccgatcgtc acggcaatgt ttatcgcgaa
    12061 cagatggtgg actttatcct tagcgcgttg aatccgcaga actaacccat gatcgctagc
    12121 acgataatca ttcacaaaac caccttaaga catgctaatc cactggtcag aacagtttaa
    12181 aatgagaaaa attctgtgac gcttgccaac atttctgatg attagcattc ccttcgccat
    12241 ttccctgagc aaactttagc tattcttatc aattatgctt atgggagatc tacagatgat
    12301 gcctacgctt gctccaccat ctgtcctttc ggctccccag cgccgctgcc agatcttgct
    12361 gacgctcttt cagccggggt tgaccgccac catggcaacc ttcagcgagc ttaatggtgt
    12421 ggatgatgat attgccagtc ttgatatcag cgaaacagga cgggagatcc tgcgctatca
    12481 tcaactcaca ctgacgactg gttatgacgg tagctaccgg gttgaaggta cagtgcttaa
    12541 ccaacgtttg tgtttatttc actggctacg acgtggtttc cgtctgtgtc cgtcatttat
    12601 taccagccaa ttcacccccg ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa
    12661 cttttacgac gataccaatc tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa
    12721 acgctttgaa tcgcgcgata ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca
    12781 gcaccacgct ggaattacgc ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg
    12841 ccttgaattt caggtagcgc aggaaattgg acgccactgg cagcgtcggg cgctccagca
    12901 tgtaccgcct gatgagccac tgtttatggc attacttttt tccatgttgc gggttcccga
    12961 tccactgcgg gatgcgcatc agcgggacag acaattgcgt cagtctatca aacgtctggt
    13021 aaaccatttt cgtgagctgg gaaatgttcg tttttatgat gaacaggggt tatgtgatca
    13081 gctttatatc cacctcgccc aggcgttaaa tcgcagtttg tttgccatcg gtattgataa
    13141 taccctgccg gaagagttcg ccagactgta tccacgcctg gtacgcacca cccgcgcggc
    13201 gctggccgga tttgaaagtg aatacggcgt ccatctttct gatgaggaaa gtggtctggt
    13261 cgcggtgatt ttcggtgcct ggctaatgca ggaaaacgat ctgcatgaaa aacagattat
    13321 tctactgacc gggaatgata gcgagcgaga agcgcagatt gagcagcagt tacgcgaact
    13381 aacgttactg ccgctcaaca ttaagcatat gtcggtaaag gcatttttgc agacaggcgc
    13441 tccgcgtggc gcggcactga ttattgcgcc ttataccatg ccgttaccgc tcttttcacc
    13501 accgctgatc tatacggacc tgacgctgac aacacatcaa caggagcaga tccgcaaaat
    13561 gctcgaatca gcatgaggga acaactttcg ggcgcaaaaa cattgccgga agcgccacca
    13621 gcgccattac ccagaacacg ccgtggccca gatattgata caggaaaccg gcgaaaacgg
    13681 tcatgatagc gatactgccg cccatcgcga cggcagagta aaccgcctgt aaacggatga
    13741 cttcgctaac ctggcgagca gcaatataac gcatggcggc caggtggcag accgtgaagg
    13801 tgccgcaatg cagaatttgc accactatca accacggcaa cgccgtagtt gctcccataa
    13861 tgccccagcg cactacgccg caaatcgccg agatcaacag catatcgcga gcactacaac
    13921 ggcggaaaag tttattactc agcgcaaaga taatgacttc cgccaccacg cccagcgacc
    13981 acaaataccc caccgccgag gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac
    14041 cgtaataggc cgcatgtgcc ccctgcaata aacaaacgca ggccagaaag cgccagttct
    14101 ggcgaaccag cgccaaccac gcagaccaac cggtgctctc ctgctggcgg cttgcccctt
    14161 gtggctgaat cgtcggacgg atgagaaagc cgagcagcat ggatgccacg cccaacgtca
    14221 acagcacgag gatcacccga taatcaaaca tagtgaccag tttgcccgtc agcgccgagc
    14281 caatgacaaa cgccaccgag ccccacagtc gcactttgcc gtaatcaagc gggaactgct
    14341 tttgccacgt attcgccagt gcatcggtca acggtaccag cggtgagaaa aagaggttaa
    14401 agccaatcat caccagcatc agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa
    14461 agagaagtgg cagcagtgcc agcacgcgca aggcggaaat caggcgggaa ggatcgctga
    14521 cgcggggcgc gatgagcaaa ctcccgagaa aacgggcaac cagacctgcc cccaataaca
    14581 ggccgatggt ttctggcgtt aaaccaatcc ctttaagcca gacgctccag aaaggtaaaa
    14641 aaatgccgta actaaaaaag tatgtgaaat agccgagcgc caaccagcgc gtggattgca
    14701 aaaccatgag tccctcccgt caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa
    14761 gttgaaaaat aagaatattc gagcctgctt cccgatgtct gtaacgccag ttcacaagca
    14821 ctaaaaaact gcgatccgcg acgcatgttg caaaaataat gcgtaattat gccgttacgc
    14881 ttgccaaacg ttcctgcaca atggcgatga aatcgcgtaa tgccagcttc atctcccctt
    14941 tcttccacgc catcagtaaa gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa
    15001 cctgcccggt attaaaatta ttcatataac cgggtatcaa agtgacgccc agccccatgc
    15061 ccaccagatt catggtcacc agaatattcg ttgccacctg gacgatattt ggctggctgt
    15121 tttcttgcgc aaaccacgct ttaacgatcg gcgcaagcga accggaatac gccggatcgg
    15181 tactgacgaa attcacgcca tccagttgcg cggcggtgat ctctttttca tgtgctaaag
    15241 ggtgatcaac cggtaacacc accactaatg gttcgtcaaa aagctccaga taatcaatct
    15301 ccgggctata aacaggatgg cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt
    15361 cctcctgttg cgtggtgatt aaactcacca gctcaatcaa ggtgtctggc tgtctgagac
    15421 gaaacatcgg taatactttt ggcaataaat tcacttccgc cgatggcaca aagccaatgg
    15481 ttaattgtct gtcttcctga acaattttcc gcgcccgtaa tttggcattt tccgcttgtt
    15541 cgaggattgc cagcgcgtcc tggagaaaac attctcccgc cgccgtcaac gcgactttgc
    15601 gcttatccct caccagtaac ggaacaccga cacagttttc aagatcgcgg atctggctgc
    15661 ttaacgaagg ctgtgaggta tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg
    15721 ccactgcgac gaaatagcgt aaatgccgta gttccatcac cttccccttg ttatcgaaaa
    15781 aacgtctcag ctggtagaaa ttaactattt cacatattag caaccaacca gcaacatcct
    15841 tatggcacaa aaatagaagg tcaatacatc ttatctttca ggattaaaaa atatgaccac
    15901 accctcagat ttgaacattt accaactgat tgacacccaa aacggtcggg tcactccgcg
    15961 tatttatacc gacccggaca tttaccaact ggagcttgag cgtattttcg gtcgttgctg
    16021 gctatttctc gcccacgaaa gccagatccc aaaacccggt gatttcttta acacctacat
    16081 gggagaagat gcagttgtcg tggtgcgcca gaaagacggc agcatcaagg cgtttctcaa
    16141 ccaatgccgc caccgggcca tgcgtgtgag ttatgcagat tgtggcaaca gtcgcgcctt
    16201 tacctgcccg tatcacggct ggtcttatgg cattaacggc gagttgatcg atgtaccgct
    16261 ggaacctcgc gcctacccac aagggttgtg taaatcccac tgggggctaa acgaagttcc
    16321 ttgtgtggag agttataaag gactgatttt tggcaactgg gataccagcg caccgggcct
    16381 gcatgattac ctgggtgaca ttgcctggta tctggatggc atgctggatc gtcgcgaagg
    16441 cggcaccgaa attgtcggcg gcgtacaaaa gtgggtgatc aactgtaact ggaaattccc
    16501 ggcagagcag ttcgccagtg accagtatca tgctctgttc agccatgctt ctgccgttca
    16561 ggtattaggg gcgaaagatg atggcagcga taagcgcctc ggtgatggac aaaccgcccg
    16621 cccggtgtgg gaaaccgcca aagatgcgct gcaatttggt caggacggtc acggtagcgg
    16681 tttcttcttt actgaaaaac cggatgctaa tgtctgggtc gatggcgcag tttcaagcta
    16741 ttaccgcgaa acctatgccg aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct
    16801 ggcgggtcat aacaatatat tccccacgct ttcatggcta aacggcactg ccacactccg
    16861 cgtctggcat ccgcgcggcc ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa
    16921 agccgcctcc gatgaagtta aagccgcttt tgaaaacagc gccactcgtg cttttggtcc
    16981 tgctggtttt ctcgagcagg atgactcgga gaactggtgt gaaatccaga aattgcttaa
    17041 aggccaccgc gcccgcaaca gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg
    17101 ccgcgacgac ggcattcctg gcattactaa ctatatcttt tcagaaactg ccgctcgtgg
    17161 aatgtaccaa cgctgggccg atcttctgag tagcgaaagc tggcaggaag tgctcgataa
    17221 aaccgccgct taccagcagg aggtgatgaa atgagtgcgc aagtttcact ggagttacat
    17281 caccgcatta gccagtttct ctttcacgaa gccagtctgc tggatgactg gaaatttcgt
    17341 gactggttgg cacagctcga cgaagagatt cgttacacca tgcgcaccac ggttaacgcg
    17401 caaacacgcg accgtcgcaa aggcgtccag ccgccgacga cctggatttt caatgacacc
    17461 aaagaccagc tggagcggcg aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag
    17521 ccgccgtcac gcactcgtca cttaatcagc aactgccagg taagcgaaac cgacatccca
    17581 aacgtatttg ccgtgagggt gaattatcta ctttatcggg cacaaaaaga gcgcgatgaa
    17641 acattctatg ttggaacgcg tttcgacaaa gttcgccgtc tggaagatga caactggcgc
    17701 ttgctggaac gggatatcgt cctggatcaa gcggtgatca cttcccataa cctgagtgta
    17761 ctgttctgat gaatcgaatt tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc
    17821 tccggataga tacctcgccc gtcatcgcct tgttcaacgt gggcggcgag ttttatgcca
    17881 ttaacgatcg ttgcagccat ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg
    17941 ccacggtgga gtgcccgcta cacgccgcca gtttttgcct gaaaacgggg aaagcgttat
    18001 gcctgcccgc caccgatccg ctcaccactt atccagtaca cgttgaaggt ggtgacattt
    18061 tcatcgactt accggaggcg cagccatgag cgatctgcat aacgagtcca tttttattac
    18121 cggcggcgga tcgggattag ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca
    18181 agttgccacg ctggaactgt cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga
    18241 acatattctg gcggtggaag gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga
    18301 tcagatcctg actcgttccg gcaagctgga ttgttttatc ggcaatgcag gcatctggga
    18361 tcacaatgcc tcactggtta atactcccgc agagacgctc gaaaccggct tccacgagct
    18421 gtttaacgtc aacgtactcg gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat
    18481 cgccagtgaa ggcagcatga ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg
    18541 tggcccgctg tacaccacca gtaaacatgc cgcaaccgga cttattcgcc aactggctta
    18601 tgaactggca ccgaaagtgc gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct
    18661 gcgcggccca caggcgctcg ggcaaagtga aaccccgata atgcagtctc tgacgccgga
    18721 gaaaattgcc gccattttac cgctgcaatt tttcccgcaa ccggcggatt ttacggggcc
    18781 gtatgtgatg ttggcatcgc ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc
    18841 tgatgcgggt ttagcgattc gcggcattcg ccacgtagcg gctgggctgg atctttaagg
    18901 aagcacgatg aaagaaaaaa cgatcattat tgtcggtggc gggcaagcgg cggcaatggc
    18961 tgcggcctcg ctacgccagc aagggttcac cggtgagctg catctgtttt ccgatgagcg
    19021 acatcttcct tatgaacgac ctccactctc gaaatccatg ttgctggaag attccccgca
    19081 gttacagcag gtgttacccg ctaactggtg gcaggaaaac aatgttcatc tgcattccgg
    19141 tgtaaccatc aaaacgctgg gtcgcgacac acgagagtta gtgttaacca acggcgaaag
    19201 ctggcactgg gatcagcttt ttatagcaac cggcgcggca gctcgaccgc tgccgttgct
    19261 tgatgcactg ggagaacgct gctttaccct acaccatgcc ggtgatgccg ccagactgcg
    19321 agaagttctg cagcccgaac ggtcagtcgt gattgtcggt gccggaacta ttggtctgga
    19381 actggctgcc agcgccacgc agcgcagcgc agcgcagcgc agcgcagcgc agatgtaagg
    19441 tgacagtgat tgaactggcg gcaaccgtca tgggccgtaa tgcaccaccg cccgtgcaac
    19501 gctatctttt acagcgccat cagcaggctg gtgtgcgcat tctgctcaat aatgccattg
    19561 aacatgtggt cgatggtgaa aaagtagaac tgacgctgca aagtggggaa acgcttcagg
    19621 ctgatgtggt gatttacggt attggtatca gcgccaacga gcaactggct cgcgaggcca
    19681 accttgatac tgccaatggc attgtcattg atgaggcttg ccgcacctgc gatcccgcga
    19741 tctttgccgg tggcgatgtg gcaatcactc gtcttgataa tggtgcacta caccgctgcg
    19801 aaagctggga aaacgccaat aaccaggcgc aaattgccgc tgccgcaatg ttagggctac
    19861 cgctaccgct actgccgccg ccgtggttct ggagcgatca gtacagtgat aacttacagt
    19921 ttattggcga tatgcgtggc gatgactggc tttgtcgtgg caacccggaa actcagaagg
    19981 cgatttggtt taatctgcaa aacggcgtgc ttatcggtgc ggtcacgctg aatcagggcc
    20041 gtgagattcg cccaattcgc aaatggatcc agagcggcaa aacgtttgat gcgaaactgc
    20101 tgatagatga gaacatcgcg cttaaatcac tgtaatcagg ataattagcg aatatctcaa
    20161 tgcctggggc gtggcgaggt gcaagagtgt gtattacgtt taaatcacat tatcttgcaa
    20221 agggattggt tatgaacaca ctacgttatt ttgattttgg agctgcccgc cccgttttgt
    20281 tattaattgc ccgtatcgcc gtggtcttaa ttttcattat ttttggtttt cccaaaatga
    20341 tgggctttga cggtacggtc caatatatgg cctcgttggg cgcgccaatg ccgatgctgg
    20401 cagcgattat tgcggtagtt atggaagtgc ccgccgcgat attaatcgtg cttggctttt
    20461 tcacccgtcc gctggcggtg ctgtttattt tctacacgct gggtacggcg gtgattggtc
    20521 accattactg ggatatgacc ggcgatgcgg ttgggccaaa tatgattaat ttctggaaga
    20581 atgtcagtat cgctggcgcg ttcttgctat tggcaattac cggaccgggg gcaatttctc
    20641 tcgatcggcg ttaggaaaga tgccggatgc ggcgtgaacg ccttatccgg cattttataa
    20701 attacagcca ctctacccgc aacgacatct ctgaggttgt tgattcccct ggtgctagcg
    20761 caatgagatc acccccttcc gggcgatgat gctcatccgg cgcatggctc atcggttcca
    20821 gacagaagaa atcaaacgca tatcctttat caaacgcagg gtcggaaaca aagatgaaat
    20881 aacacggtgc aggtggcgtc gtttccatga tgatggcata accttcctgc ggctgctcga
    20941 tgcgggcctg accattccat ccggcaaaac cattgttcac ccactgacgc ggcaacggcg
    21001 cgagctggtt aaaatccagt tcctgcggta gctgctcgca aaactcaccc gccagccact
    21061 gctcccgttc cagccagtaa ccgctcgcct gcgcctgaat ccgcgtttgt ggtgacaacg
    21121 gaaaataagg atgccagccg gtaccaaatg gcagcgtctc tgccccttga ttggtgacag
    21181 agagcgtcac cgtcagcgta tccgccgtta aatgaaacgc ctgacttacc cgatagtgat
    21241 agacaccgct gcgatgttca tacaccagac acaaactatc atcgctgtgc gagacacatt
    21301 gccactcgcc cagccagcca tcgccgtgta gatagtgcgc atcccactca acattcggtt
    21361 gcagttgata ctcacgcccc tgccagacaa aacggttgcc gctcacccgg tttgcaaatg
    21421 gcaccagcgg aaaacatgag gcatcggttg ccacaccgct ttttttacca ggacgtaaga
    21481 gcggcgtcgt atcgcgccag aagccttcga ttacgccgcc ctggtcagaa acgtccagct
    21541 ttagcgaccc gtgggataag gtatagatgg tcatccgcta actccttaat ccgggaagtt
    21601 aatcacaact ttcccgcatt tgccgctcgc catcagcaca taggcatcac ctgcctgttc
    21661 tagcgagaag cgatgggtaa tggcgttacg cggccacagc ttccagtcag tcaaatcgtg
    21721 ggcgcatttt tccatatgga acagactggt cacccaggag ccgataatcc gccgttgatg
    21781 gtgcatcaga tcagcgctga cctcgaattc cacttttccg gtttcaccaa tgtaaaccac
    21841 ccgcccccag tcagcagtgg attgcagtgc cagcaagcga cctgcggcat taccggaaca
    21901 atcgagcgca acatccgcgc caccgtgggt aagttcagca attatctgcg gcagaccttc
    21961 ggtggttgct aaatagccgt gatccatccc ccctaactgt tttgccatcg ccagacgttc
    22021 cggcagcata tcaacgccga tgatccgttt tgcaccgcga cctttcgcca gcatcatcgc
    22081 catcatgccg accggcccca gaccgaccac cagcacgtta tcgctgccgg aaacttcgcc
    22141 gcgcaaaatc ccttcatagg ccgtgccaac gccgcaactg ataaacgcac catcttcgta
    22201 gctcagcgca tccggcagga ggatcagatc tttttcttcc gccagcagat attcggcatg
    22261 accgccgtca cgctgccagc cgtaagccgc ttttcctttg ccagtacaag agataggaaa
    22321 gccgcggcgg cagttcgggc aaaaaccaca gccagaaatg tgatacacca gcacgcggtc
    22381 gccctcttta aaatggcggc agccttgccc catcgccaca atctgcccgc acggttcatg
    22441 accgttgata aatccctggt ataacggttt atcgggtgcc gccgccgtgg cacggtgttg
    22501 atgatagata tagtggacat cgcttccgca aatcccggag gatttcattt tgatcagtac
    22561 ctggttaatc cccggcgtcg gcaccgcaac ttcccgcaga tcgacggttg aatttcctag
    22621 taaataagca gccagcatcg ttttcataaa tccctcatta acaatacgat taattttcat
    22681 ccctgcagca cccgcgccag gggcgttaag gtttagcgtt tcgctttact gcttcgctgg
    22741 gtcagcaaga tattcgccag caccgccacc acgatgatga cgccgcgtac cacctgctgg
    22801 aaaaaggagt taataccgag cagcaccaga ccgttaccga ttagcgtaat caccagcaca
    22861 ccaagcaatg taccgaacag ggaaccgcga ccgccggaaa gtgccgtacc gccgaccacg
    22921 accgcggcga tgacgtcaaa ctccagaccg tttgcggcac ctgcgttacc ggaaccgagg
    22981 cgtgccgcca acaaaatgcc ggtcaccgcc gctaataatc ccgaaagggt aaagataaga
    23041 atgcgcactc gacgaacgtt aataccgcac aactgcgccg ccgtggcatt accgccaacg
    23101 gcaaaaaccg agcgcccgaa ggcggttttt cggctaatga acacaaacag cgcaaacaac
    23161 accatcatga tcagcgcgga tactggcaca ccgagaaatt gtccgcccag ccagtccagc
    23221 acctcgtttt cgtcaatcgg cactggcagt gcgttggtca taaacagccc cattccgcgc
    23281 agagcgctcc acagccccag tgtggcaacg aaacttggta cgttaaacac gccgcgcagc
    23341 accccggcga gcgttcccat cagcgcgcct aacagcaaca ccagcagaca agcaatcgcc
    23401 aacggaactt caaattgcag caaaaatgcc aggcacaccg agacaaaagc caccatcggc
    23461 ccaacgctga catcaatttc accggagata ataatcagcg tcatcgccca ggcggcaatc
    23521 ccaatggtgg cggcatcgcg cagcacgttc atctggttat tcaatgagat aaagccaggc
    23581 gcgttcaggg agaagaccag ataaagaatg gcaatcacca ccagcaaacc gatctcatta
    23641 atatggcgac tgacaaattg tttgagcgag acgctcttgc cctgcggcaa tagtaatgac
    23701 gaagcataca tcttcaggtt cctcggtaaa attcatcagt gcacagacag aatggcggac
    23761 atcagctcat ccacattgac cggcgagtga aactcctgcg agaacgtgcc gtgctgtaat
    23821 aacaggatgc ggtcacacac cagcggcaat tcctccactt cactggagat aaacaccacg
    23881 ctttttcctt cggcagccag ctcacggaca atacggtaaa tctgctgttt ggcttcgata
    23941 tcgacgccgc gcgttggctc gtcgagcaac aaaatctggc tggcggcata gacccaacga
    24001 ccgatcacca ctttttgctg attgccacca gaaagcgtgc cgatgggtgt ttcgctactg
    24061 gcagccttga ccgtcatccg ctgcatcacc tcttcggtca ggcggcggat ggtggaccat
    24121 tgcagcacac cgttggtgct gattttttgc cgattggtca gcactgtatt ttcgtcaacg
    24181 cctagccagg gaatgatccc cgcttctttg cggttttctg gcgtatagcc aatgccgcgt
    24241 ttcagcatgt cgccgtaatc ggggcgcatg attttctcgc cgttgataac aatttcgccc
    24301 tgttcatacg tctccagccc aacaatcgcc ttcagcaatt cactgcgccc tgcccccagc
    24361 aggccagcaa tgccaagcac ttcgccacga cgtagcgtaa agctgatatc ctccagcttg
    24421 ggcttatggc gtaacgcacg gacttccagt acggcctgat ccacaatttc ctgaggggca
    24481 accggcgcaa tatcaacgtg atcacgccct agcattagcg acacaatatg atgcgtggag
    24541 gtgttttcga gcatcacatc gcccgccacc tgaccatcgc gcataacggt ggcataggag
    24601 gcaatgcggc ggatttcttc catccggtgg ctgacataaa tcaccgccac gcccagtgcc
    24661 gacatctttt tcaccgcact gatcaccagt tcaacttctg cactggcaag cgagctggtg
    24721 ggttcatcaa gaatgaccac gcgcggctcg cccttcatca cccgtgcaat ttccaccagt
    24781 tgcttttgcg ccgggcttag cgttgaaaca agttgttcag gactaacgtc aacgcccagc
    24841 gcctgtaagc aacgttgggc atcctgcgcc atttgcaggt aatcaatcat gccgttgcgg
    24901 cgaggccatt gaccaaggca gaggttttct gccaccgtca gcccttccac cagacttaat
    24961 tcctgataaa ccgcacgaac ccccagttca gcggcacggc gagtcaacgt agcttcgtca
    25021 ccttccagtc gcgtctcgcc aatccagata tcaccgctat ccgggcgttc gctgccggta
    25081 agcattcgaa tgagagtcga tttgcccgcg ccgtttttac ctaacagcgc acgaacttcg
    25141 cctttattga gcgtgaagtt aacgttatcc agcgcaacga cgccggggta acgcttattt
    25201 cctgccacca cttttgctac cgggactgcc tctgttgccg tgaacatagg gacctctgcg
    25261 aatcagcgat tagggcagag cagaccatca acgtgcgttg ccagccactg tttaccatct
    25321 tccgttttgg tatagagatc gattggcacc tgaatcactt tttcaccgtc ggcttgttta
    25381 ttgataacct tcaatgtttg cgcgaaaaca gcattgccca ttttcttacc ggaaatatcc
    25441 actaccgctt tcagcacctg attgttttcc agctcctgag caatttcggt tgtcatatcc
    25501 gaaccgaaaa cagcaatttt tccggcctga ttttgattac gtaccgcttt taccgcgccg
    25561 agtgtcgcac cgcccgactc ccccataatg gcgttgagat ccggcgtgga gataatcagt
    25621 ttttcaccaa cggaaatcgc tttatctaaa acagtccctt cctggttagc gacaatttgc
    25681 gcgccgggaa cgcgggattt taatacttct tcaaatcctt tacgtcgctg cacacaaact
    25741 tcaaaggctt cgcaattgat gacggcaatt ttcggctggt caattttatt ggcaataaaa
    25801 taatcggcgg cagcgttacc cagtttttac caaattccag tggatcgccg accagatccg
    25861 ccgagacata tttatcgaca cccttttgat taatacaggt gttgtagcaa atcaccggaa
    25921 tacccgcttc actggcgcga cgaacggtac ggctactgcc attttcagac actgccgata
    25981 aaataatggc atcgacatta cgcgccacga gggtatcaac aaaggtactt tctttcgaaa
    26041 tatccccctg ggcgttagtt tcaattaact gtacctgtac tgaagaatct tttgccgcat
    26101 cctgaacgcc ctgacgcact ccagcgtaat atccctgggt atcaaggtat attgcaccaa
    26161 tggtcatttc tttatccgca gccctggcaa atagtgcgct acctaatagc gtagccatta
    26221 ataatagatt acgggttgtt ctcatttttt taggcatagt gcctcctgta gggtttttat
    26281 taacaacggc ttattctaat tattttgtga tgagcggcag cgcgtgccgc tcaaatatta
    26341 cagttaatag ggtttagtga acgtagttaa aaataaaagg catcacagtg gtgaataatg
    26401 ccgctttcgg ccaggccgga ttaatcgccg tcaattcagc gcgagcagac tcatattgcg
    26461 caacctcccc gagtcccgcg gacgccagca tcgccaccat caggcatttt tctttatgct
    26521 gctgttgtaa atcgccatac agcgataaca ggtcaggttg tgaaacagca aagaaatccg
    26581 cctcaatact ggttttcgcc atctcttgcg cccactgttt catttcacta aacagttgct
    26641 gtgcggtttg ctgttcaccc agcagtcgca gcgccattcc ttgccagaag agataatcaa
    26701 ccggctgatc gttgtaataa ctgtggatgt taatggtgcg atcgccggtc gccgccagac
    26761 gtaaacaacg catcgcttca gtttcatcgc cctgcgcgtt ggcgcatatc gcctgccaga
    26821 accagatgtc gttatcagtt tgccccggta aacggccttc gcttaaattc tccggataat
    26881 acagcgcggc atgaagcagt tcgctggcct gctgcggctc tctggcatca agatgctgcc
    26941 aggcgcgtaa taactggttg aggataaact gactggtgac cttcccttcc ccgccttccc
    27001 acgggtggaa tttacgcgtg gcgagaatgt ccgccgcttt gtctgcctga cccgtgagat
    27061 gccacaaatt gagcagttct gcggtcatgt cgtcgcgttt cagcgcaatt tccagattat
    27121 tttccagacg cgccagtcgt ttctccggtg tggctccact taacttatcc agcaaatccc
    27181 gttcgaaaag cagacgtgca tcctgcggcg caagctgata agcattatca agataacgcg
    27241 cggccagctc ataatcgtgt ttcttattcc acgcatggat cgctaacccg cgccagccgt
    27301 cggcaaactc cggcgacatc tctacgcaac gttgccataa ggcaatggct ttgttgtagc
    27361 tacgtttgtt gtagtagaag caggccagta aatggcgagc aaaccaacac tcttcaatac
    27421 tctccagcgc cgccacttct tccagcgtat tcgggaaacg gacaaactgc gggaagacat
    27481 caatggcttt tgcgaccagt tcgccacgtt cggctttcgg cagcaagctg gcttgcaggt
    27541 aaagcggcag cgtgcgctgg cagtccagtg cgttcagcat ctctgccgcc agggtgggca
    27601 ttccccagtt aatcagttgc ccggcggtca tcagagcgtt aacgtcgcgt ccctgacaca
    27661 gaccgcgcca ctgcgcgagg gctgattcgc tacgaccatc gaaccagttc agccaccaca
    27721 gagtggcgtt cagcggataa tcacgcagca gtttctcgcg ctgcaaacgc gcgttgtcct
    27781 gacgaccact taacaccagc agcagattat gcaagcaaag cacttcctga ttggttgggc
    27841 aggcacgaag actttgttgg caaaaatcca gacccgcgtc gaagttatca ttacgcgccg
    27901 ccagtcgtgc cagaccataa tagccgcctg ctttactatt gccgctccag accgcacgcc
    27961 agaaatcctc ttcggcttgt tgatattgtc cctgacgttc gtaagcactg gcgcgaatca
    28021 aacttgcttg tccacactgc ggttttttgt tcagcgcatg tgcacgtttc agcgcctgac
    28081 tggcatacgc caccgcttgc gggaaatctg cgcggttata ttccagcatc gccagcgcca
    28141 agttacagcg ataatccagc gggtccagcg ccacgccgcg caggtagtaa tcgaacggtg
    28201 aacggctggc gtgatgatat tgctccagat gctgaccgat aaaccaggct tcatctgtac
    28261 tggtaatgtc ttgtgctgcc agtggcgctt tggcgacgtc cggcaacggc aactcctgcg
    28321 gctgatgttc ctgataactc agtacaatat tgccgtcggc atcggagagt tcaatggtca
    28381 gtctgtcagg attgataccg tgcaacacgc cctggatggc ggtcgctggt gtcagggcca
    28441 cggcatcatc gagcaacgcg ttgcatttgc cgatttcgcg gatcgccaga cggtatccgt
    28501 tcaacggaga gatggcatac agcccccact caatcccccg ctcactacgc tggagtttta
    28561 tcaccgcatc gcgggaggca ttttgcacca tgcccagaga atgataaggc aggaaatact
    28621 gctcgaaacg cttctcttcg taagcatcaa gccaggtaaa atcaggctgg ttatcggcaa
    28681 aaataccggt catcagttcg atatacgggc cgttattgtc ggtcagactc ttatcccacg
    28741 cctggccaaa ttcactgtgt ccccaactcc actgtttttt acctggcgca atatggtggt
    28801 tggcaacgtg cagcaaaccg ccatcttcat cgtgacacca cgcgccgaca aaatcgtact
    28861 gcgatttttc ggccatatat gaggttggaa caggcacatt tttatagcga gaaatgtcca
    28921 ctccggcgga gtagtccact ttgtagtaag tgccggtggc gatggggaaa gcggagacgg
    28981 cccgtttgcc gtgatcaaac accgcagtca catccggtgg gaaaacgctc tgatgccctt
    29041 cacccccttt cactgccggg ttgacccacc acaagaaatg acgcggcgtg gcgttgccgt
    29101 tatagacgcg gctggcgatt tccagtgccg cccggtcagg gcgcagggtg aaacctgtca
    29161 tcacctgtaa accatgcatc ggctccgttt cgccgaccca caccgtctgt gcaccgtctt
    29221 catgggcttc gagggtgaaa tcaacgggca taaaggtggt cgggcgatgg tgttgcggcc
    29281 agttaaactc aatcccgccg gagatccacg gtcccagcat ccccaccagc gcaggtttaa
    29341 tgacttcatt gtgatagaca aaatcgcgct gtttcacttt atcccatgcg cgatgcaccc
    29401 gaccgcccag ttccggcagg atcatcactt tgatgtagtc gttttccagc cacaccgcct
    29461 gctaggattt cagggttttc tgctcgctca gcgtatcggt cacgccgtag ggataaaccg
    29521 cgccggacga tccctgataa acgcgatttt ccaggaacat gggatgtata tcctgcggcc
    29581 cggtttcata ggtcgggatc tcaacgcgct cttgccacac ttttactgga gtcatggtgc
    29641 cctcaatatt aacaagacat actgaattaa aagatttgtg gcagtgtatt gaacaatctg
    29701 gcaatgtttt cgcggaataa tcacgcaatt aactaaacaa ggtttagtga agatgagagc
    29761 ctgcattaac aatcaacaga ttcgccacca taacaaatgc gtgattctgg aactgctgta
    29821 ccggcaaaag cgcgccaata aatcaacgct ggcccggctg gcgcaaattt cgattccggc
    29881 agtcagtaat attttgcagg aactggaaag cgaaaaacgg gtggtgaata ttgacgatga
    29941 aagccagacg cgcgggcata gtagcggtac atggctgatt gcgccggaag gtgactggac
    30001 gctgtgcctg aacgtgacgc ccaccagtat tgagtgtcag gttgctaatg cttgtttaag
    30061 tccgaaaggc gaatttgagt atttacagat tgatgtaccg acgccgcagg cgctgctgtc
    30121 cgaaatcgaa aaatgctggc atcgccaccg taaattgtgg ccggaccgca ccatcaatct
    30181 ggcgctggca atccacgggc aggttgatcc ggtgaccggc gtatcgcaaa ccatgccgca
    30241 agcgccgtgg acaacgccag ttgaggtgaa gtatctgctg gaagagaagc tgggcattcg
    30301 ggtgctggtc gataatgact gcgtgatgct ggcgctggcg gagaaatggc aaaataattc
    30361 gcaggaacgg gatttctgcg tgatcaacgt tgattacggc attggctcgt cgttcgtgat
    30421 taacgagcaa atttatcgcg gcagtttgta tggtagcgga cagattggtc acaccatcgt
    30481 taatccggat ggagtcgtct gcgactgtgg acgttacggc agcctggaaa ccgtcgcctc
    30541 gttaagtgca ttaaaaaaac aggcgcgggt atggctaaaa tcacaaccgg ttaatactca
    30601 gcttgagcct gaaaaactga caaccgcgca gttaatcgct gcctggcaga gtggagaacc
    30661 gtggatcacc agctgggttg atcgctctgc caatgccatt ggtttgagtc tgtataactt
    30721 cctcaacatc ctcaatatta atcagatttg gttgtacggt cgcagttgtg cctttggtga
    30781 gaactggctt aatactatta ttcgccagac aggatttaac ccgttcgacc gcgacgaagg
    30841 accgagcgtg aaagcgacgc aaattggctt cgggcaatta agccgcgcac aacaggtgct
    30901 gggaattggc tatttgtatg ttgaggcgca gttacgacag atttgatggc gcgataacgt
    30961 agaaaggctt cccgaaggaa gccttgatga tcataaacga aaaattgcct gatgcgctac
    31021 gcttatcagg cctacacgga gattgcaata tattgaattt gcaagatttt gtaggcctga
    31081 gaaggtgttc atgccgcatc caacatgaac aacgagcaca ttgtcagcaa atcaccgttt
    31141 cgcttatgcg taaaccgggt aacgtgcgca gatgtcgaga actttacctt tgatgcgctc
    31201 gataacggct tcatcattga tgctgtccag cacgtcacac atccagccag ccagttcttt
    31261 cgcttcggct tctttaaagc cgcgacgggt aatcgccgga gtacctacac gaataccgga
    31321 ggtcacaaac gggctcttcg gatcgttcgg tacgctgttt ttgttgacgg tgatgttagc
    31381 acggcccaga gcggcgtctg cttctttacc ggtcaggttt ttatcaacca gatcaaccag
    31441 gaacaggtgg ttatcagtgc cgccggaaac cactttgtag ccgcgctcga ggaacacttc
    31501 taccatcgct ttagcgtttt tcgcgacctg ctgctggtaa gttttgaact caggctccat
    31561 cgcttctttc agagcaaccg ctttaccggc gattacgtgc atcaacggac cgccctgacc
    31621 accagggaaa acggcagagt tcagtttttt gtacagctct tcgctaccac ctttcgccaa
    31681 gatcaggccg ccgcgcggac ccgccagggt tttgtgagtg gtggtagtaa caacgtgagc
    31741 atgaggaacc gggttcgggt agacgccagc agcaaccagg cccgcaacgt gcgccatatc
    31801 aacgaacaga taagcaccga tgctgtcagc gatttcacgc attttcgccc agtcaaccac
    31861 gccggaatat gcagagaaac caccgataat cattttcggc ttgtgttctt tggcttgttt
    31921 ttccagatcg gcgtaatcga tatgaccggt agcatcgata ccgtaaggaa cgatgttgta
    31981 cagtttaccg gagaagttaa ccggagaacc gtgagtcagg tgaccgccat gcgccaggtt
    32041 catacccaga acggtatcac ctggttccag cagcgcggtg tagaccgcaa agttagcctg
    32101 ggagccggag tgcggctgga cgttagcgta gtcagcgccg aacagttctt tcgcacgatc
    32161 gatcgccagt tgttcaacga tatcaacata ctcgcaaccg ccgtagtagc gtttgcccgg
    32221 ataaccttca gcatatttgt tggtcagctg agaaccctgc gcctgcatta cgcgcgggct
    32281 ggtgtagttt tcggaggcga tcagttcgat gtgctcttcc tgacgtactt tttcctgctc
    32341 catagcctgc cacagttcgg catcataatc ggcaatgttc atttcacgct ttaacatccg
    32401 catctcctga ctcagctaac aataaaattt ttggccttta taggcggtcc tgttggacaa
    32461 cggcgaacag tataaccgaa tcattgtgcg ataacaggtc ttgacaaagg aatttacgca
    32521 aacgattacc ttcaggctac gcaaggcttt ggagaataaa gagcttgcaa ccggaaacgg
    32581 atttcttttc aggtttgtga tgcaaatttt tcacttcatc acattctttc tgaaaaacac
    32641 caaagaacca tttacattgc agggctattt tttataagat gcatttgaga tacatcaatt
    32701 aagatgcaaa aaaggaagac catatgcttg acgctcaaac catcgctaca gtaaaagcca
    32761 ccatcccttt actggtggaa acggggccaa agttaaccgc ccatttctac gaccgtatgt
    32821 ttactcataa cccagaactc aaagaaattt ttaacatgag taaccagcgt aatggcgatc
    32881 aacgtgaagc cctgtttaac gctattgccg cctacgccag taatattgaa aacctgcctg
    32941 cgctgctgcc agcggtagaa aaaatcgcgc agaagcacac cagcttccag atcaaaccgg
    33001 aacagtacaa catcgtcggt gaacacctgt tggcaacgct ggacgaaatg ttcagcccgg
    33061 ggcaggaagt gctggacgcg tggggtaaag cctatggtgt actggctaat gtatttatca
    33121 atcgcgaggc ggaaatctat aacgaaaacg ccagcaaagc cggtggttgg gaaggtactc
    33181 gcgatttccg cattgtggct aaaacaccgc gcagcgcgct tatcaccagc ttcgaactgg
    33241 agccggtcga cggtggcgca gtggcagaat accgtccggg gcaatatctc ggcgtctggc
    33301 tgaagccgga aggtttcccg catcaggaaa ttcgtcagta ctctttgact cgcaaaccgg
    33361 atggcaaagg ctatcgtatc gcggtgaaac gcgaagaggg tgggcaggta tccaactggt
    33421 tgcacaatca cgccaatgtt ggcgatgtcg tgaaactggt cgctccggca ggtgatttct
    33481 ttatggctgt cgcagatgac acaccagtga cgttaatctc taccggtgtt ggtcaaacgc
    33541 caatgctggc aatgctcgac acgctggcaa aagcaggcca cacagcacaa gtgaactggt
    33601 tccatgcggc agaaaatggc gaagttcacg cctttgctga tgaagttaag gaactggggc
    33661 agtcactgcc gcgctttacc gcgcacacct ggtatcgtca gccgagcgaa gccgatcgcg
    33721 ctaaaggtca gtttgatagc gaaggtctga tggatttgag caaactggaa ggtgcgttca
    33781 gcgatccgac aatgcagttc tatctctgcg gcccggttgg cttcatgcag tttaccgcga
    33841 aacagttagt ggatctgggc gtgaagcagg aaaacattca ttacgaatgc tttggcccgc
    33901 ataaggtgct gtaatttgat gttgccggat ggaaacatcc ggcaaccctt gacgcggttt
    33961 aaattgccgc gtcgtcctct tcaccggtac ggatgcgaat gacccgagcc acgtcaaaga
    34021 cgaagatttt accgtcaccg attttgccgg tttgcgccgt gcgaataatg gtatcgacac
    34081 aggtatcgac aatgtcgtcc ggtacgacaa tctcaatttt cactttcggc agaaaatcca
    34141 ccatatactc cgcgccgcgg tacagctcgg tatggccttt ctggcgacca aagcctttca
    34201 cttcggtcac cgtcatgccg gtaataccga cttcggccag tgcttcgcgg acatcgtcca
    34261 gcttgaaggg ttttataatc gcatcaatct ttttcatgct attccttgaa aaggttgcct
    34321 gtcttttgat ctgctaaacg taacacataa cgccaattca ttccttgaaa tcgtttgcat
    34381 ccagctcgtg tcgggaaagc agtttataaa attctgtccg gttgcgcccc gccattctcg
    34441 ccgcgtgggt gacgttgcct ttggtgattt gcagcagctt acgcaagtag ttgagttcaa
    34501 aatgattacg tgcctcaaca aaggttggca gcgccgtatt ttcaccctcc agcgcctgct
    34561 ccaccagcgc atcactaatc accggagatg aggtcagcgc cacgcactgt tcaatcacgt
    34621 tgaccaactg gcgcacatta cccggccagc tcgcggtcat caggcgtttc atcgcatcgg
    34681 tagagaacgc gcggacaaac ggtttatgtc gctctgccgc ctggcgcaac aggtgatttg
    34741 ccagtagcgg aatgtcttct gtgcgctccg ccagtgccgg aattttcagg ctgacaacgt
    34801 tgaggcggta atagagatct tcgcggaatt ccccgcgcgc catcgctttt gacagatcac
    34861 ggtgagtggc agaaataatc cgcacattga tatcaatatc gcggttactg cccagcgggc
    34921 gcactttacg ctcctgcaac acgcgcagca gtttgacctg taacggcgca ggcatatcgc
    34981 caatttcatc gagaaatagc gttccgcctt ccgccgcctg gaataaacct tcgcgattgc
    35041 tgacagcgcc agtaaacgcg ccacgcgcat gaccaaacag ctccgactcc agcaattgct
    35101 cgggcaatgc gccacagtta atagcaataa atggtttgct gttgcgcggg ctggcgttgt
    35161 ggatagcctg ggcgaaaatc tctttcccgg tgccgctctg accgttaatc aaaacgctga
    35221 cgtctgattg cgccaccagc cgcgcctgtt ccagcaaacg cagcatcagc gggctgcggg
    35281 tgacaattgc ctcgcgccag cgttcatcgg tggctggcgc ggattgctcc agcgcatcgt
    35341 caattgcctg atatagcgca tctttatcga caggcttggt gaggaaacta aaaacgccct
    35401 gctgtgttgc agcaacggca tcggggatag aaccatgcgc agtaagaata attactggca
    35461 ttcccggctg cactttctgg atttcagcaa atagctgcat accgtccatt tcatccatcc
    35521 gcaggtcgct gataactaaa tctacttttt cgcgattcag tacccgtaat ccttcagcgc
    35581 cactttccgc cgtgaccaca ctgtagcctt cgctggtcag gcgcaggcca agcagtttca
    35641 gcaatcccgg atcgtcatcg accaataata aatgcgcagg tttatggctc atcaggagtg
    35701 acctcatggg tggatggcgc gggcttttca ctctcgtgcg gcgtatccgg cgagaaattt
    35761 ccagccggtt tgcgggtcga gagctggcgt tcaatatcgg tcaggttttc cagcttgcgg
    35821 gtggtgagtt ccagttgctg ctgtagaacg tgatgttgct ggcgcaatgt atccagctcg
    35881 ctgtcgctgg actgctggag tttactgtaa cgttggcgct cttccgccag ttgcagttgc
    35941 agcgcctgac cgtcgcgcca gagttgatac agtgggcgaa cctgtgccgg gatctcggta
    36001 cttaacgctt caatacgcgc gaccagctgg cggcgctcat acggcgtaat tttggcgtcg
    36061 gcgagcaaaa tcccttgttt aaaggtattt tgccagctgc cgtcgtcata ttggcgagct
    36121 tgctgacgcg actgcgcagg cattaaacga tcagcacaat ccatcgcccg cagccagtaa
    36181 agcggattgg tttcggttga tttaccttgc agcgcccaga tgtcgctaca ttcagtagaa
    36241 agatagtcag ccagttgata aaccggaatt ttttcttctg ctggcgtatc aatggctggc
    36301 ttattgtgat tctgcacgca acccagcaat gccagacatg gcagccctgc cagccacagc
    36361 cgtcggggca ataatcgttg aaaaatgtgt cgcatattca ccagacttaa agcctatccc
    36421 agtgggcgta attgttgcag acagtctgga catggacagc gcggagaaac cggggcgtac
    36481 atatcgtacg tgaagatttc gagcactacc cggggccgaa ctgacaaata aaatagcctg
    36541 ataggatagg ctgttagcat tatttcgtgt ttttcgacga cggtaattca atgcggaaac
    36601 aaacgtcttg cccgctctcg tcgaccagat acagttcccc ttgcatacgg cgaatacaat
    36661 ccctggcaat gcttaatccc agaccgctgc ccttcaccgc cccttttcgc tggtggcttc
    36721 cctgaaaaaa gggttcgaag atcatggcgc gttcctcttg cggaatgggc gtgcctgtat
    36781 tgatgacatc aatataaacc cgcgcaccat gtaaactgct gcgaaggcaa atgttaccgg
    36841 attcagcccc gtagtgcacc gcattggagt aaagattatc cagtacgctc atcagcagca
    36901 ttggctccgc caggcaagct gttgctttga gatcgacgtc ggtatgcatc attttagccc
    36961 gtgcgggcag gctatgagca gaaaccactg tctccaccag cggtgctaat tcaacattct
    37021 ccagttccac cgcactgtcc gcctgtttac ggttgtaatc aagcagttgt tcgatcagtt
    37081 tttgcaaatt gcagctgctg ctatcaagaa tgctcaccac ctctttttgc tctggcgtaa
    37141 gcggcccgac aacctgatca gccagtaatt cagtgccctc gcgcatactc gccagtggcg
    37201 tttttaattc atgagataaa tgtcttaaaa attgatggcg ttgggattcc agccatgaca
    37261 ggcgctcact taaccaaaga atacgttgcc caaccgagcg taactcgctc ggtccacaga
    37321 acgaaacgct attgcccaga gaacgccctt cccccagacg gttgatcatg cgctcgatat
    37381 ttttcaccgg cccgataatc atccgcgtga aaagtagcac catcaccaga ctcaccagaa
    37441 atagcaccag cgattgccaa ccaaaatatt gcccacgttc ggcgatttca cgctgaagtt
    37501 gctgcccacg agagaacacc actgtgcgcg tggcctgcac catttcggta ttggcactgg
    37561 caaaggcttc aagacgcgcg gcggcggcag catcgggacc actgttgtta cactgaagtt
    37621 gggccagatt gttcaaatcc tgacgcaatg cctggtaaag tttatcgtct ggcagtacgc
    37681 ctgcgtgggc atcgagcatt tcgctgtaac gcttgcgctg gctttgataa accttcgcaa
    37741 gcgtagggtc gtccagcacg caatactgac ggtagctacg ctccatctcc agcgccgcgt
    37801 tggtcatcgc ttcactacgc cgggcatcaa taagcgtagt gcggttaacc agcgccgcct
    37861 gatcgctaag cgcattcagg ctttgccagg cttgccatgc cagcaccaac aggggtagta
    37921 gaattagcaa aaatgccagc attaccagtt gtcgtaatga gcggggaaaa acgggccagc
    37981 gtttcaaggt gttactctcg tcagacgcga atagcctgat gctaaccgag gggaagttca
    38041 gatacaacaa agccgggaat tacccggctt tgttatggaa taaggcggtg cctaactcga
    38101 cgtttcgccc gatggttgat atagctacgc tgatatcaga agttggacgg caggcacctt
    38161 gttgtgcgtc attcgaattt tatgtagcac gtcccgaagg ggctgacata agtcggtgaa
    38221 tgagccactg cttactatta tgcagtaact gtgccaataa agaaaatagt ttggtaacgt
    38281 actgattata cgttgctttg agggatttat ggcttcttcg ctatttgaat gtaaatcagc
    38341 cactgtgtcg ctaaaaagag acaacttaag ataaacttat tagattacaa taaaatcaat
    38401 catttaagtg tcgccagaaa gcgacacggt aaaccaccca ttgtcgtgat ttacagacac
    38461 aaaaaagctc ccggagttgg gagcttatga tagtggttgg tgcttaatgc cgcatccggg
    38521 ctgcaaaacc aatgaactga tgacttaccc caactgctta cgcgcattgc ggaaaatgcg
    38581 catccatggg ccatcctcgc cccagttttc cggatgccag gagttgctga cagtacggaa
    38641 aacacgttcc gggtgcggca tcataatggt gactcgacca ctttcagtcg taaccgccgt
    38701 aataccgttc ggtgaaccgt tcgggttagc cgggtaggtt tcagtgactt tgccgaagtt
    38761 atcgacatag cgcagtgcca ccagcccttt gctttccagt gccgccagat gcgccgcatc
    38821 acgcacttcc acgcgccctt caccatgaga gacagcaatc ggcatttgcg agcccaccat
    38881 cccctgcaac agcagagacg ggctttgggt tacttcaacc aggctgaaac gcgcttcaaa
    38941 gcgatcggag gtgttacgca caaaacgtgg ccacaactca ctccccggga tcagttcacg
    39001 caggttagac atcatctggc aaccgttaca tacccccagg gccagcgttt gcggacgatg
    39061 gaagaaggtt gcaaactcat cgcgtacacg gtcattgaac aggatagact tcgcccaacc
    39121 ttcaccggca cccagcacat caccgtagga gaaacctccg cacgcgacca gggcgtgaaa
    39181 atcctccagt cccgtacgtc cagtcagcag gtcgctcata tgcacgtcga tagcatcaaa
    39241 gcccccacgt tggaaagctg ccgccatttc aacatgcgag ttcacgccct gctcacgcag
    39301 tacggcaact ttcggacgtg cgccagtggc aatatacggt gctgccacat cttcgttgat
    39361 atcgaacgac agttttacat tcaggcccgg atcggcgtcg ttagatttcg cctgatgctc
    39421 ctgatcggca cactccgggt tgtcacgcag gcgctgcatc tgccaggtag tttctgccca
    39481 ccagacacgc aacgtagtgc ggctttcgct gaatacagtc tgcccgttgg cggtaatcac
    39541 aaaacggtca ccggaaaccg cctgcccaac ataatggacg caatccgcaa gcccatgctg
    39601 tgccagtacg gactcgaccg cttcacggtc agcggcacga acctgaatca ccgcacccag
    39661 ttcttcgtta aacaacgccg ccaggcgatc gtcacccaga gaggcgatat ccgcgtcaat
    39721 gccacaatga cctgcaaagg ccatttccgc cagcgttacc agcaggcctc catcagagcg
    39781 gtcgtgatac gccagcagct tacgctgtgc aaccagcgcc tgaatcgcgt catagaagcc
    39841 tttcagttgg acaacatcgc gtacatctgc cggtttgtcg ccaagctgac gataaacctg
    39901 cgccagcgcc gttgcgccca gcgcgttatt gcctttgccc aaatcaatca gcagcagtgc
    39961 gttatcttcg gtacaaagct gcggcgtgat ggtgtgacgt acatcttcca cgcgggcaaa
    40021 tgcagaaatc accagcgaca gcggcgacgt catttcgcgc tcttcgttac cttcctgcca
    40081 gcgggttttc atcgacatag agtctttacc caccgggatc gtcaggccca gcgccggaca
    40141 aagttcttca cccacggctt taacggcttc atacaggccc gcatcttcac cagggtggcc
    40201 tgccgccgcc atccagttgg cggaaagttt aatgcgtttg atatcgccaa tctgtgttgc
    40261 ggcaatattg gttaacgctt caccgaccgc cagacgggca gaggcggcga aatccagcag
    40321 cgcaaccggc gcacgctcgc caatcgccat cgcttcaccg tagtagctgt cgaggctggc
    40381 ggtagtgacc gcgcagttag cgaccggcac ctgccacggc cccaccatct gatcgcgcgc
    40441 caccatgccg gttacgctgc ggtcaccgat ggtcaccagg aaagttttct ccgccacggt
    40501 cggcagatgc agtacgcgtt tcaccgcgtc tgcaatggtg atcccttcac ggaccagcgc
    40561 atcgcccttc gctttcagcg tttgtacatc gcgggtcatc ttcggcgttt taccaagcag
    40621 gacgtccagc ggcagatcga tcggctgatt atcaaaatga cgatcgtgca gagaaagatg
    40681 cagttcttcg gtcgcttcac caatcaccgc gtagggtgcg cgctcacgct tacacagttc
    40741 gtcaaacagc ggtaattgat cggcagcaac cgccagcacg tagcgttcct gggattcgtt
    40801 acaccagatt tccagcgggc tcatgcccgg ttcgtcgctt agaatatcgc gcagttcaaa
    40861 tttaccgccg cgtccgccgt cgctcaccag ctccggcatc gcgttagaaa gaccgcccgc
    40921 accgacgtcg tggataaaca gaattgggtt agcatcacca agctgccagc aacggtcgat
    40981 cacttcctgg caacgacgct ccatctccgg gttgtcgcgc tgtacggaag caaagtcgag
    41041 gtcggcatca gactgaccag acgccatgga agacgccgcg ccgccgccaa gaccgatgtt
    41101 cattgccggg ccgccgagaa cgaccagctt cgcaccgacg ttgatctcgc ctttttgtac
    41161 gtgatcggcg cgaatgttgc cgatcccgcc cgccagcatg atcggtttgt gataaccgcg
    41221 cagctcttcg ccattgtggc tgttcacttt ttcttcataa gtacggaagt agccgttcag
    41281 tgccggacga ccaaattcgt tgttaaacgc cgcaccgccc agcgggcctt cggtcatgat
    41341 gtccagcgcg gtaacaatgc gctcaggctt accgaaatct tcttcccacg gctgttcgaa
    41401 gccaggaatt cgcaggttgg atacggagaa accaaccaaa ccagctttcg gcttcgcgcc
    41461 gcgcccggtg gcaccttcat cgcggatttc accgccggaa ccggtcgccg cgcccggcca
    41521 cggagaaatc gccgtcgggt ggttgtgagt ttcgactttc atcaggatgt gcgccggttc
    41581 ctgatggaaa ttgtagcggc ccgtttcgtg gtcagcaaag tagcggccca cttcagaacc
    41641 ttccattacg gcggcgttat ctttataagc agagagaacg tgatctggcg tggtttcgaa
    41701 agtatttttg atcatcctga acagcgattt cggctgttgt tcaccgtcga taatccagtc
    41761 ggcgttaaaa attttgtggc ggcagtgctc agagttcgcc tgagcgaaca tatacagctc
    41821 gatgtcgttc gggttacgac caagctttgt gaaagcatcc tgcagatagt caatttcatc
    41881 ttccgccaga gccaagccaa gacgcaggtt agcgtcgatc agcgcctgac ggccctgccc
    41941 cagcaaatca acgcttgtga ccggagtcgg ttgatggtgg gcaaacaact gctctgcatc
    42001 atctaaatca aaaaagaccg tttccatcat gcggtcgtgc agttcagcgg taacctgctg
    42061 ccattgttca ttggtcagcg taccggcttc tatatagtaa gcaacgccgc gctcaaggcg
    42121 gtttacctgt tgtagcccgc agttatgggc aatatcggtc gctttcgaag accagggaga
    42181 gatggtgcca ggacgcgggg tcaccagcag gagtttgcct tgcggggcgt ggctggcgag
    42241 tgccggacca tatttcagca ggcgttcaag ttgtgcgtgc tcatcatcgt ttaacggcgc
    42301 attgaggtca gcaaaatgga catactcggc gtaaatattg tgaaccggga gcctggcagc
    42361 ctgaaaacgt gccagcagtt tgttgattcg gaatgccgac agtgcaggcg aaccacgcag
    42421 aatttccatc ataagtctct cgtcttctaa gctttcggtg tacccaaggg gggggaaacg
    42481 ggcgtcatta taaagaatct gatgcgctga cgaaaccgtt tgcgtggaaa taaaatcacc
    42541 atcgtgaatt agcaacgcgt gccgccaatg gctgtaataa gttgccatct ggcgcaggtt
    42601 tacgcaaaat gccgctcatt tatgagtaaa cctttcacta ttattacgtt ttttcaagct
    42661 gggacgcgca cgacacagag aattaactaa ttgaaaaaat taaagattaa ttatctgttc
    42721 atcggcattc tggcactgct gctcgcggtc gctctctggc catccattcc ctggtttggt
    42781 aaagccgaca accgtatcgc cgccattcaa gcgcggggag agttgcgtgt gagcaccatt
    42841 catactcccc tgacttataa cgaaatcaac gggaaacctt ttggcctgga ttacgaactg
    42901 gcgaaacagt ttgccgatta cctcggcgta aaactgaaag tgaccgtgcg gcagaatatc
    42961 agccagctgt ttgacgacct tgataatggt aacgccgacc tgctggcggc aggactggtc
    43021 tataacagtg agcgggtaaa aaattatcag cctggcccta cctattattc cgtgtcacaa
    43081 caactggttt ataaagtggg tcagtatcgc ccacgtacgc tgggcaacct gacggcggag
    43141 caactcaccg ttgcaccggg tcatgtggta gttaacgatc tccagaccct gaaagaaaca
    43201 aaattcccgg aattaagctg gaaggtagac gacaaaaaag gctctgcgga attaatggaa
    43261 gatgtcatcg aaggaaaact cgattacacc attgctgatt ctgtcgccat cagcctgttt
    43321 cagcgcgttc acccggagct cgccgtagcg ctcgatatca ccgatgaaca accggtgact
    43381 tggtttagcc cgttagatgg cgataatacc ctttccgccg ccctgctcga cttcttcaac
    43441 gaaatgaatg aagacggtac gctggcacgc attgaagaga aatacctggg gcatggcgat
    43501 gattttgatt acgtcgatac gcgcacattt ttacgcgccg tcgatgcggt actgccgcag
    43561 ttaaagcccc tgtttgagaa atacgccgaa gaaattgact ggcgtttgct ggccgctatt
    43621 gcttatcagg aatcgcactg ggatgcacag gccacttcac cgacgggtgt gcgcggcatg
    43681 atgatgttaa ccaaaaatac cgcgcaaagc ctcggcatta cggatcgtac cgatgccgaa
    43741 cagagcatca gcggtggcgt gcgttatttg caggatatga tgagtaaagt gccggaaagt
    43801 gtgccggaga acgaacggat ctggtttgcc ctcgctgcgt acaatatggg ctatgcgcat
    43861 atgctggatg cccgcgccct gacggcaaaa accaaaggga atcctgacag ttgggctgac
    43921 gtaaaacagc gtctgccttt acttagccag aaaccctatt acagcaagct gacttacggc
    43981 tacgctcgtg ggcatgaagc ctacgcttat gtcgaaaata ttcgtaagta tcagattagc
    44041 ctggtgggtt atctgcaaga gaaagagaag caggctacag aagcggcgat gcaactggcg
    44101 caggattatc cggcggtatc gcctacggag ttgggcaaag agaaatttcc ttttctctcg
    44161 tttctttccc agtcgtcatc aaactatttg acccattctc cctctctgct gttttccagg
    44221 aaagggagtg aagagaaaca aaattaatcc gtcgaggatt gcgctttttt ctgcgcttta
    44281 atttcctggc ggcgcatgcg aaagaagtca ctgagcaacg ccgcgcactc atccgccagt
    44341 attccttccg taatttccac tcggtgattc atacccggat gatgcagcac atccattaaa
    44401 gatcccgcag cgccagtttt cgcgtcacgc gcaccaaaga ccacgcgacc aatgcgactg
    44461 tggatcatcg ctccggcaca cattacacat ggttcaagcg tgacatacaa cgtggcgtcg
    44521 atcagacgat aattttgcat caccagacca ccctgccgca gggccatgat ttctgcatgt
    44581 gcggtgggat catggcgacc aatcgggcgg ttccagcctt cgccgattac ccgattgtta
    44641 tgcactaata ccgcgccgac cggcacttcc cgctcatccc aggcacgttt cgccagcgtc
    44701 agcgcgtgac gcatccaata ttcgtggcta aattcgactt cagacaaaaa gaaaactccg
    44761 gttataaaag cgcggcgcat tatacacgga cgctatgctt tactctattc cagttgctgg
    44821 agttcaccgc gcggggttac tcgccaacga tgctgacaga aataaagcag cggattgtcc
    44881 tgattactgt cgctatagcc actgtacagc cgcagcggcg tgccgatttt gcgctccagt
    44941 tgtgcgacct tttcatgtcc cagacaacgc atcgtcaata cccaaccacc atagccacgc
    45001 tgaatttggc tggcgataag attaacccgc ggcagccagg gcgtatcgaa ataaaccgct
    45061 tcaaccagcg gctgcggaga gccggtaatc aaccagatat cagcatcgga acttaacagg
    45121 taggtggtta atcgctcctg aaccagcgga aaggcggtaa cattgtcgcg aaaccagcgc
    45181 acgaaatcgg cctgcaacgt ctgtaaacgt gcttcgctgt gaccaaaagt gcacccccac
    45241 agaagcagac tcatcggcca gcgtgccgca cgacctttta tcaataacgc aatggctata
    45301 atcggtaaca acggcaggac aagtaacgca ttcagcggtt ggcgacgcag taaatagcgc
    45361 agaaaactgc cgaacatatc ctgctgatgc aatgttccat ctaagtcaaa aaacaccaca
    45421 cgacgctcgt gagttgccaa accatactcc tctggactga aacatcctga ttaattttcg
    45481 ctactacata gcctaacaga tagatcatca cttttccggc aacaatcgac cgcatcatgg
    45541 ctaactggaa tttttaattc atggcaatta gcggcaatgg aatataaaat tcactcgcgt
    45601 gtgtctcata ttgcctgttg tcgccactat ttcatcgcga ggaaagatat gaacggctta
    45661 cttcgtatcc gtcagcgtta ccaagggctt gcccagagtg ataaaaaact ggcggattat
    45721 ctgctgctac aacctgatac ggcgcgccat ttaagctctc agcaactggc caacgaagcc
    45781 ggagtcagtc agtccagcgt cgtgaagttc gcgcaaaaac tcggctataa aggttttccg
    45841 gcgcttaagt tggcgttgag tgaagcgctg gcaagccagc cggaatcgcc ctccgtgccc
    45901 attcataacc aaatccgcgg tgatgatccg ttacggctgg tcggcgaaaa actgattaaa
    45961 gaaaataccg ccgctatgta cgcaacgcta aacgttaata gtgaagagaa actgcatgaa
    46021 tgcgtaacaa tgttgcgctc tgcgcggcgg ataattctga ccggtattgg cgcttcgggt
    46081 ctggtggcgc aaaactttgc ctggaagctg atgaagattg gcttcaatgc tgccgcactg
    46141 cgcgatatgc atgcgctact cgcaacagta caggcgtcgt cccctgacga tctgttatta
    46201 gccatttcct acaccggtgt acgacgcgag ttaaacctgg cggcagatga gatgctgcga
    46261 gtgggcggaa aagtgctggc gattaccggc tttactccga atgccctgca acagcgtgct
    46321 tctcattgcc tgtataccat tgccgaagaa caagcgacaa acagtgcttc aatctctgct
    46381 tgtcacgctc agggaatgtt aacggatttg ctgttcattg cgctgattca gcaggatctg
    46441 gaactggcac cagaacgtat tcgtcatagt gaagcgctgg tgaaaaaact ggtctgagta
    46501 aagaatgcgc gtataatgcc cgcccggttt gtgttgtttt gagagtttcc ttatggcgct
    46561 gttaatcact aaaaaatgca tcaattgtga tatgtgtgaa atcaaataaa aacacattta
    46621 ttttcatagt gatacagcaa tgtgttattg aatgtgttgt tggatttatg gtgcaaaaaa
    46681 attttgcata agttcagcaa cacaaagaac accactttct ttaacgtcct ccgcgcccta
    46741 aaggacgggg ctttacggcg caaaaggcct gtgacataca acattaactt ttcagtcaac
    46801 cgtagcacgt tcttgtatac gccgtgtctg cggttttata taaccctggc tggaaacctc
    46861 ttatacaaag tcgattcgcc aacatcatag atgatcgcca ccttctggcg agaaactcct
    46921 gatgcaatta gtcgcccggc ctgcgcccat tattctggtg aaagtttggg acggcgaccg
    46981 ccaattcgtc cctgtgcgcg agcagcttcc agtcctgctt ttgttcgttc aacaatcagt
    47041 tcacgttcca tttcagccag ggcacccatc acatggaaga aaaagcgtcc cattggtgtg
    47101 ctggtatcaa ttgaatcagt cagactacga aagttgatgc ctcgttcgcg caacgcctcc
    47161 accagcacga caagatgccg catactgcgc cccagccgat ccagcttcca gacaaccagt
    47221 gtgtcgcctg ccgataatgt cttgagcagt tttttcagcc ccggtctttc ggactttgta
    47281 ccacttatct tgtcttcaaa aatcagctcg catcctgcac agttcaacgc attacgttgt
    47341 agatcggtat tctggtcatt tgt
//
LOCUS       DAJTRD010000025        45785 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTRD010000025 DAJTRD010000000
VERSION     DAJTRD010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45785
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.025"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            95..2248
                     /locus_tag="OO315_001511"
     CDS             95..2248
                     /locus_tag="OO315_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5890471.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="OO315_001512"
     CDS             2301..4031
                     /locus_tag="OO315_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890472.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OO315_001513"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OO315_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5890473.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="OO315_001514"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="OO315_001514"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5890474.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="OO315_001515"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="OO315_001515"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5890475.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OO315_001516"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OO315_001516"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5890476.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="OO315_001517"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="OO315_001517"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5890477.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="OO315_001518"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="OO315_001518"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5890478.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="OO315_001519"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="OO315_001519"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5890479.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="OO315_001520"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="OO315_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5890480.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OO315_001521"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OO315_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5890481.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OO315_001522"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OO315_001522"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5890482.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OO315_001523"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OO315_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5890483.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OO315_001524"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OO315_001524"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5890484.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OO315_001525"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OO315_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5890485.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OO315_001526"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OO315_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5890486.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="OO315_001527"
     CDS             16289..16828
                     /locus_tag="OO315_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5890487.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /gene="tsx"
                     /locus_tag="OO315_001528"
     CDS             17126..18010
                     /gene="tsx"
                     /locus_tag="OO315_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5890488.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /locus_tag="OO315_001529"
     CDS             complement(18187..18534)
                     /locus_tag="OO315_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5890489.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OO315_001530"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OO315_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5890490.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..21492)
                     /gene="secD"
                     /locus_tag="OO315_001531"
     CDS             complement(19645..21492)
                     /gene="secD"
                     /locus_tag="OO315_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5890491.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="OO315_001532"
     CDS             complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="OO315_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5890492.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="OO315_001533"
     CDS             complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="OO315_001533"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5890493.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23057..24127)
                     /gene="queA"
                     /locus_tag="OO315_001534"
     CDS             complement(23057..24127)
                     /gene="queA"
                     /locus_tag="OO315_001534"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5890494.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24220..24793
                     /gene="acpH"
                     /locus_tag="OO315_001535"
                     /pseudo
     CDS             24220..24793
                     /gene="acpH"
                     /locus_tag="OO315_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="OO315_001536"
     CDS             complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="OO315_001536"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5890495.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26771..28144)
                     /gene="proY"
                     /locus_tag="OO315_001537"
     CDS             complement(26771..28144)
                     /gene="proY"
                     /locus_tag="OO315_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5890496.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="OO315_001538"
     CDS             complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="OO315_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5890497.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="OO315_001539"
     CDS             complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="OO315_001539"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5890498.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="OO315_001540"
     CDS             complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="OO315_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5890499.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32178..33380
                     /gene="sbcD"
                     /locus_tag="OO315_001541"
     CDS             32178..33380
                     /gene="sbcD"
                     /locus_tag="OO315_001541"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5890500.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33377..36520
                     /gene="sbcC"
                     /locus_tag="OO315_001542"
     CDS             33377..36520
                     /gene="sbcC"
                     /locus_tag="OO315_001542"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5890501.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36646..37827
                     /gene="araJ"
                     /locus_tag="OO315_001543"
     CDS             36646..37827
                     /gene="araJ"
                     /locus_tag="OO315_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5890502.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37970..38878)
                     /gene="mak"
                     /locus_tag="OO315_001544"
     CDS             complement(37970..38878)
                     /gene="mak"
                     /locus_tag="OO315_001544"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5890503.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39003..39914
                     /gene="rdgC"
                     /locus_tag="OO315_001545"
     CDS             39003..39914
                     /gene="rdgC"
                     /locus_tag="OO315_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5890504.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39992..40447)
                     /locus_tag="OO315_001546"
     CDS             complement(39992..40447)
                     /locus_tag="OO315_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890505.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40444..40779)
                     /locus_tag="OO315_001547"
                     /pseudo
     CDS             complement(40444..40779)
                     /locus_tag="OO315_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="OO315_001548"
     CDS             complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="OO315_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5890506.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41290..41967)
                     /locus_tag="OO315_001549"
     CDS             complement(41290..41967)
                     /locus_tag="OO315_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5890507.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="OO315_001550"
     CDS             complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="OO315_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5890508.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="OO315_001551"
     CDS             complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="OO315_001551"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5890509.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43173..43631)
                     /locus_tag="OO315_001552"
     CDS             complement(43173..43631)
                     /locus_tag="OO315_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5890510.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43751..44560
                     /gene="proC"
                     /locus_tag="OO315_001553"
     CDS             43751..44560
                     /gene="proC"
                     /locus_tag="OO315_001553"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5890511.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="OO315_001554"
                     /gene_synonym="dgcC"
     CDS             complement(44577..45692)
                     /gene="adrA"
                     /locus_tag="OO315_001554"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5890512.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
BASE COUNT        11902 a        11944 c        11201 g        10738 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgataccagg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    21181 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    21241 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    21301 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    21361 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    21421 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    21481 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    21541 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    21601 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    21661 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    21721 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21781 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21841 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21901 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21961 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    22021 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    22081 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    22141 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    22201 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt
    22261 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    22321 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    22381 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    22441 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    22501 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    22561 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    22621 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    22681 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    22741 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22801 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22861 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22921 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22981 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    23041 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    23101 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    23161 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    23221 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    23281 agatttgggt atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc
    23341 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    23401 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    23461 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    23521 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    23581 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    23641 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    23701 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    23761 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23821 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23881 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23941 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    24001 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    24061 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    24121 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    24181 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    24241 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    24301 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    24361 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    24421 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    24481 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    24541 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    24601 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    24661 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    24721 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24781 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24841 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24901 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24961 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    25021 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    25081 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    25141 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    25201 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    25261 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    25321 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    25381 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    25441 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    25501 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    25561 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    25621 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    25681 tcattcacca gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag
    25741 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg
    25801 tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg
    25861 tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa
    25921 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    25981 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc
    26041 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    26101 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    26161 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    26221 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca
    26281 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    26341 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    26401 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
    26461 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    26521 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    26581 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    26641 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    26701 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    26761 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    26821 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    26881 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    26941 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    27001 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    27061 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    27121 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    27181 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    27241 cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg
    27301 caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct
    27361 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
    27421 gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg
    27481 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    27541 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    27601 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    27661 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    27721 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    27781 gcaccgtcgg gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    27841 caataaggat ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt
    27901 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    27961 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    28021 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    28081 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt
    28141 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    28201 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    28261 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    28321 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    28381 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    28441 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    28501 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca
    28561 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    28621 caaagaattc tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga
    28681 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    28741 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga
    28801 acagcgccag gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat
    28861 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    28921 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    28981 aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    29041 tcgcgacaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    29101 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    29161 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    29221 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    29281 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    29341 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    29401 cgaatgccgc agtccagacg tgttcgcctg cctgtaaacc gaccattgga gggaaaataa
    29461 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    29521 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    29581 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    29641 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    29701 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    29761 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    29821 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    29881 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    29941 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    30001 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    30061 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    30121 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    30181 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    30241 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    30301 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    30361 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    30421 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    30481 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    30541 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    30601 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    30661 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    30721 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    30781 tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag
    30841 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    30901 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    30961 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    31021 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
    31081 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
    31141 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    31201 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    31261 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    31321 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    31381 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    31441 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    31501 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    31561 gatgtcgggt tgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa
    31621 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg
    31681 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    31741 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    31801 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    31861 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    31921 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    31981 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    32041 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    32101 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt
    32161 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    32221 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    32281 cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    32341 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    32401 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    32461 atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    32521 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc
    32581 gtgacattat ttccagccag gcggggctta acggtattga aaaacagcag catttactgg
    32641 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    32701 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg
    32761 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    32821 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    32881 ttcgctattg cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg
    32941 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    33001 taactcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    33061 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    33121 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    33181 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    33241 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagacc
    33301 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg
    33361 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    33421 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    33481 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    33541 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    33601 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    33661 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga
    33721 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    33781 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    33841 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    33901 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    33961 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc
    34021 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    34081 aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    34141 gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa
    34201 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    34261 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga
    34321 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    34381 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    34441 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    34501 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    34561 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    34621 ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    34681 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    34741 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    34801 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    34861 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc
    34921 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    34981 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    35041 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    35101 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    35161 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    35221 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    35281 tcaacagcaa attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact
    35341 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    35401 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    35461 accgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    35521 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    35581 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga
    35641 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    35701 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    35761 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    35821 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    35881 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    35941 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    36001 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    36061 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    36121 ccggctgcac gtgcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    36181 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    36241 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    36301 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    36361 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    36421 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    36481 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    36541 tacagagggg tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    36601 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    36661 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg
    36721 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    36781 tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    36841 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    36901 acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat
    36961 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    37021 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    37081 ctgggaacgt atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt
    37141 aatattgcgg tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa
    37201 ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc
    37261 gccacgatgt ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg
    37321 atgtttattt ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta
    37381 gggatggtgc tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc
    37441 attgcagcag tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc
    37501 ggcatgaaaa caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt
    37561 tcagcacctc tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc
    37621 gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg
    37681 atgctgacgc tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt
    37741 gctgcgatgt catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt
    37801 ccggtgctgg cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg
    37861 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa
    37921 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    37981 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg
    38041 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata
    38101 aacggtctac attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca
    38161 cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta
    38221 ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca
    38281 aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt
    38341 taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta
    38401 gcggattgtg tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga
    38461 atgccacgcc cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg
    38521 ctgccgcgcc atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca
    38581 cttcccgctg caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg
    38641 aattgacatt cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta
    38701 ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa
    38761 tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg
    38821 catcgcccag tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg
    38881 tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc
    38941 aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta
    39001 tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg
    39061 cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca
    39121 tggcgaagat gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg
    39181 ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga
    39241 ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga
    39301 agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt
    39361 tcagccgttt tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg
    39421 actgcgccag tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt
    39481 cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg
    39541 ttcgctccgg tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt
    39601 tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca
    39661 ccaatcacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca
    39721 ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc
    39781 gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca
    39841 tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg
    39901 aagcacaacg ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta
    39961 tccggcgtac gaagccacac caggcatata attattcgct acggcgagca ataattttta
    40021 gcgcagcaat attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc
    40081 tgtcagcagc acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta
    40141 acagctccgc ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat
    40201 ccgtcaggta aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg
    40261 caataaagtc tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg
    40321 gtaaatcggt acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca
    40381 ggttgatcgt tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt
    40441 gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc
    40501 ggttttcact aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga
    40561 tggatcctct tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc
    40621 cagcgaccga tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt
    40681 gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc
    40741 atcatctgat ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact
    40801 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
    40861 aggaaatatt attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg
    40921 ggaaggcgag gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat
    40981 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
    41041 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    41101 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    41161 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    41221 actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt
    41281 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
    41341 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    41401 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt
    41461 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    41521 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    41581 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    41641 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    41701 ctgattatcg agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac
    41761 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    41821 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    41881 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    41941 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    42001 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    42061 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg
    42121 ttattgttta atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc
    42181 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg
    42241 tcctcatagc gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac
    42301 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    42361 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    42421 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    42481 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    42541 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    42601 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    42661 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    42721 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc
    42781 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    42841 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
    42901 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    42961 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    43021 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    43081 aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata
    43141 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    43201 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    43261 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
    43321 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc
    43381 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    43441 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
    43501 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    43561 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    43621 caaattgtca taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt
    43681 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    43741 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    43801 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc
    43861 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    43921 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
    43981 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    44041 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    44101 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    44161 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    44221 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
    44281 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    44341 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
    44401 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    44461 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    44521 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    44581 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    44641 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    44701 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    44761 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    44821 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    44881 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    44941 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    45001 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga
    45061 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    45121 acccagccaa acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    45181 ggagcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    45241 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    45301 cacagaatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    45361 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    45421 gccctgctcg ctatctgcca ggctaaatgc ggccagacga acgcccagcc gaccaacacc
    45481 agccaccacc agcccggcgg cgggtgcgaa accagcgttg aagcaatcgg taagaacatg
    45541 ccagccaggc caaccgcacg gggtagtctg acgcgacggg cgaagcgcag cccggaccgc
    45601 tgatgttcgt tttgaggagt taaaggaggt tcctccccat gcgccgccgc ttttttgaaa
    45661 aagttttcat cattcattat ttttgggaac attcagaaac aattttccca aattatagag
    45721 acggggctaa aggcagaagt atgaaatttc gggtgagcca ctttcgtagc tcacccgtta
    45781 gcact
//
LOCUS       DAJTRD010000026        42396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTRD010000026 DAJTRD010000000
VERSION     DAJTRD010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.026"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..418)
                     /gene="ssb1"
                     /locus_tag="OO315_001555"
     CDS             complement(<1..418)
                     /gene="ssb1"
                     /locus_tag="OO315_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5890513.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQP"
     gene            672..3494
                     /gene="uvrA"
                     /locus_tag="OO315_001556"
     CDS             672..3494
                     /gene="uvrA"
                     /locus_tag="OO315_001556"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5890514.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3529..3885)
                     /locus_tag="OO315_001557"
     CDS             complement(3529..3885)
                     /locus_tag="OO315_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5890515.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(3889..4305)
                     /locus_tag="OO315_001558"
     CDS             complement(3889..4305)
                     /locus_tag="OO315_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5890516.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4416..5129)
                     /gene="aphA"
                     /locus_tag="OO315_001559"
     CDS             complement(4416..5129)
                     /gene="aphA"
                     /locus_tag="OO315_001559"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5890517.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5531..5735
                     /locus_tag="OO315_001560"
                     /pseudo
     CDS             5531..5735
                     /locus_tag="OO315_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6238..7431)
                     /gene="tyrB"
                     /locus_tag="OO315_001561"
     CDS             complement(6238..7431)
                     /gene="tyrB"
                     /locus_tag="OO315_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5890518.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7684..8763)
                     /gene="alr"
                     /locus_tag="OO315_001562"
     CDS             complement(7684..8763)
                     /gene="alr"
                     /locus_tag="OO315_001562"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5890519.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(8816..10231)
                     /gene="dnaB"
                     /locus_tag="OO315_001563"
     CDS             complement(8816..10231)
                     /gene="dnaB"
                     /locus_tag="OO315_001563"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5890520.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10314..11297
                     /locus_tag="OO315_001564"
     CDS             10314..11297
                     /locus_tag="OO315_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5890521.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11463..11705)
                     /gene="pspG"
                     /locus_tag="OO315_001565"
     CDS             complement(11463..11705)
                     /gene="pspG"
                     /locus_tag="OO315_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5890522.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(11839..12876)
                     /gene="dusA"
                     /locus_tag="OO315_001566"
     CDS             complement(11839..12876)
                     /gene="dusA"
                     /locus_tag="OO315_001566"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5890523.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13239..14224)
                     /locus_tag="OO315_001567"
                     /pseudo
     CDS             complement(13239..14224)
                     /locus_tag="OO315_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14542..15057
                     /gene="zur"
                     /locus_tag="OO315_001568"
     CDS             14542..15057
                     /gene="zur"
                     /locus_tag="OO315_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5890524.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15099..15308)
                     /locus_tag="OO315_001569"
     CDS             complement(15099..15308)
                     /locus_tag="OO315_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5890525.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15424..16749)
                     /gene="dinF"
                     /locus_tag="OO315_001570"
     CDS             complement(15424..16749)
                     /gene="dinF"
                     /locus_tag="OO315_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5890526.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(16822..17430)
                     /gene="lexA"
                     /locus_tag="OO315_001571"
     CDS             complement(16822..17430)
                     /gene="lexA"
                     /locus_tag="OO315_001571"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5890527.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17540..17908)
                     /gene="dgkA"
                     /locus_tag="OO315_001572"
     CDS             complement(17540..17908)
                     /gene="dgkA"
                     /locus_tag="OO315_001572"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5890528.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18079..20502
                     /gene="plsB"
                     /locus_tag="OO315_001573"
     CDS             18079..20502
                     /gene="plsB"
                     /locus_tag="OO315_001573"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5890529.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20657..21529)
                     /gene="ubiA"
                     /locus_tag="OO315_001574"
     CDS             complement(20657..21529)
                     /gene="ubiA"
                     /locus_tag="OO315_001574"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5890530.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21542..22039)
                     /gene="ubiC"
                     /locus_tag="OO315_001575"
     CDS             complement(21542..22039)
                     /gene="ubiC"
                     /locus_tag="OO315_001575"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5890531.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22262..23842)
                     /locus_tag="OO315_001576"
                     /pseudo
     CDS             complement(22262..23842)
                     /locus_tag="OO315_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24071..24991)
                     /gene="malM"
                     /locus_tag="OO315_001577"
     CDS             complement(24071..24991)
                     /gene="malM"
                     /locus_tag="OO315_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5890532.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25143..26483)
                     /gene="lamB"
                     /locus_tag="OO315_001578"
     CDS             complement(25143..26483)
                     /gene="lamB"
                     /locus_tag="OO315_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5890533.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26555..27670)
                     /gene="malK"
                     /locus_tag="OO315_001579"
     CDS             complement(26555..27670)
                     /gene="malK"
                     /locus_tag="OO315_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5890534.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28035..29225
                     /gene="malE"
                     /locus_tag="OO315_001580"
     CDS             28035..29225
                     /gene="malE"
                     /locus_tag="OO315_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5890535.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29379..30923
                     /gene="malF"
                     /locus_tag="OO315_001581"
     CDS             29379..30923
                     /gene="malF"
                     /locus_tag="OO315_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5890536.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            30938..31828
                     /gene="malG"
                     /locus_tag="OO315_001582"
     CDS             30938..31828
                     /gene="malG"
                     /locus_tag="OO315_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5890537.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32199..33674
                     /gene="xylE"
                     /locus_tag="OO315_001583"
     CDS             32199..33674
                     /gene="xylE"
                     /locus_tag="OO315_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5890538.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33718..34128)
                     /gene="psiE"
                     /locus_tag="OO315_001584"
     CDS             complement(33718..34128)
                     /gene="psiE"
                     /locus_tag="OO315_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5890539.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34254..34397)
                     /locus_tag="OO315_001585"
     CDS             complement(34254..34397)
                     /locus_tag="OO315_001585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890540.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34398..34676
                     /locus_tag="OO315_001586"
     CDS             34398..34676
                     /locus_tag="OO315_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890541.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34723..36819)
                     /locus_tag="OO315_001587"
     CDS             complement(34723..36819)
                     /locus_tag="OO315_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5890542.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(36819..37556)
                     /locus_tag="OO315_001588"
     CDS             complement(36819..37556)
                     /locus_tag="OO315_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5890543.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37553..38191)
                     /locus_tag="OO315_001589"
     CDS             complement(37553..38191)
                     /locus_tag="OO315_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5890544.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38305..38547)
                     /locus_tag="OO315_001590"
     CDS             complement(38305..38547)
                     /locus_tag="OO315_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890545.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39010..40659)
                     /gene="pgi"
                     /locus_tag="OO315_001591"
     CDS             complement(39010..40659)
                     /gene="pgi"
                     /locus_tag="OO315_001591"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5890546.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41184..>42396
                     /gene="lysC"
                     /locus_tag="OO315_001592"
     CDS             41184..>42396
                     /gene="lysC"
                     /locus_tag="OO315_001592"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5890547.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGK"
BASE COUNT        10552 a        10917 c        10404 g        10523 t
ORIGIN      
        1 gaggctgacc ccaaccgccc tgcggctgac caccaccgat attgccacct gccggagcgc
       61 caccaccctg acgaccaccc agcatctgca tggtgccgcc aacgttcacc acgacttctg
      121 tggtgtagcg atcctgaccg gattgatcgg tccatttacg ggtacgcagc tgaccttcga
      181 tataaacctg agaaccttta cgcagatatt cgctcgccac ttctgccagt ttgccgaaca
      241 gcacaacgcg gtgccattca gtctgttctt tcatctcgcc ggtcgcttta tcacgccagg
      301 attcggaagt agccagcgta atgttggcaa ctgcgccacc atttggcatg tagcgtactt
      361 ccgggtcctg acccagatta ccaacgagaa taaccttgtt tacgcctctg ctggccatgt
      421 tcgtgtctcc tgaaaaaaat cgttctgaat aagtgtaaac gcgcgattgt accattacca
      481 atagcgcttt tactatgttg tgacctcggt tccgggaaac aaacctggcc agacattgtt
      541 acacaacact ccggataatg cattccaata ctgtatattc attcaggtca atttgtgtca
      601 taattaaccg tttgtgatcg ccggtagcac catgccaccg ggcaaaaaag cgtttaatcc
      661 gggaaaggtg aatggataag atcgaagttc ggggcgcccg cacccataat ctcaaaaaca
      721 tcaacctcgt tatcccccgc gacaagctca ttgtcgtgac cgggctttcg ggttctggca
      781 aatcctcgct cgctttcgac accttatatg ccgaagggca gcgccgttac gttgaatccc
      841 tttccgccta cgcgcggcag tttctgtcac tgatggaaaa gccggacgtc gatcatattg
      901 aggggctttc tcctgccatc tcaattgagc agaaatcgac gtctcataac ccgcgttcta
      961 cggtggggac aatcaccgaa atccacgact atttgcgttt gttgttcgcc cgcgtcggcg
     1021 aaccgcgctg tccggaccac gacgtcccgc tggcggcgca aaccgtcagc cagatggtgg
     1081 ataacgtgct gtcgcagccg gaaggcaagc gtctgatgct gctcgcgcca atcattaaag
     1141 agcgcaaagg cgaacacacc aaaacgctgg agaacctggc aagccagggt tacatccgtg
     1201 ctcgtattga tggcgaagtc tgcgatcttt ccgatccgcc gaaactggaa ctgcaaaaga
     1261 aacataccat tgaagtggtg gttgatcgct tcaaggtgcg tgacgatctt acccaacgtc
     1321 ttgcggagtc gtttgaaacc gcgctggaac tttccggtgg tacagcggta gtggcggata
     1381 tggacgaccc gaaagcggaa gagctgctgt tctccgccaa cttcgcctgc ccaatttgcg
     1441 gctacagtat gcgtgaactg gagccgcgac tgttttcgtt taacaacccg gcaggtgcct
     1501 gcccgacctg tgacggcctt ggcgtacagc aatatttcga tcctgaccgc gtgatccaaa
     1561 accccgagct gtcactggct ggcggtgcga tccgtggctg ggatcgccgc aacttctatt
     1621 acttccagat gctgaaatcg ctggcagatc actataagtt cgacgtcgaa gcgccgtggg
     1681 gcagcctgag cgcgaacgtg cataaagtgg tgttgtacgg ttctggcaaa gaaaacattg
     1741 aattcaaata catgaacgat cgtggcgata cctccatccg tcgtcatccg ttcgaaggcg
     1801 tgctgcacaa tatggagcgc cgttataaag agacagaatc cagtgcggta cgtgaagaat
     1861 tagccaagtt tatcagcaat cgcccatgcg ccagctgcga agggacgcgt ctgcgtcggg
     1921 aagcgcgcca cgtttatgtc gagaatacgc cgctgcctgc tatctccgac atgagcatcg
     1981 gtcatgcgat ggaattcttc aacaatctca aactcgctgg tcagcgggcg aagattgcgg
     2041 aaaaaattct taaagagatc ggcgatcgcc tgaaattcct cgtaaacgtc ggcctgaatt
     2101 acctgacact ttcccgctcg gcagaaacac tttccggcgg tgaagcccag cgtatccgtc
     2161 tggcgagcca gattggtgcg ggcctggttg gcgttatgta cgtgctggac gagccgtcta
     2221 tcggcctgca ccagcgcgat aacgagcgcc tgttgggtac gcttatccat ctgcgcgatc
     2281 tcggtaatac cgtgattgtg gtggagcatg acgaagacgc aattcgcgcc gctgaccatg
     2341 tgatcgatat cggtcctggt gcgggtgttc acggcggtga agtggtcgca gaaggtccgc
     2401 tggaagcgat tatggcggtg cctgaatcgt tgaccgggca gtacatgagc ggtaaacgca
     2461 agattgaagt gccgaagaaa cgcgttccgg cgaatccaga aaaagtgctg aagctgacag
     2521 gcgcacgcgg caacaacctg aaggacgtga cgctcacgct gccagtcggt ctgtttacct
     2581 gcatcacagg ggtttcaggt tccggtaaat cgacgctgat taacgacaca ctgttcccga
     2641 ttgcccaacg ccagttgaat ggtgcgacca tcgccgaacc ggcaccgtat cgcgatattc
     2701 agggactgga gcatttcgat aaagtgatcg atatcgacca aagcccaatt ggtcgtactc
     2761 cacgttctaa cccggcgacc tataccggcg tgtttacgcc tgtgcgcgaa ctgtttgcgg
     2821 gcgtaccgga atcccgtgcg cgtggttata cgccaggacg tttcagcttt aacgtccgtg
     2881 gcgggcgctg cgaagcctgt cagggcgacg gtgtgatcaa agtggagatg cacttcctgc
     2941 cggatatcta cgtgccgtgc gatcagtgca aaggtaaacg ctataaccgt gaaacgctgg
     3001 aaattaagta caaaggcaaa accatccacg aagtgctgga tatgaccatc gaagaggcgc
     3061 gtgagttctt tgatgcggtg ccagctctgg cgcgtaagct gcaaacgttg atggacgttg
     3121 gtctgacgta cattcgcctg gggcagtccg caaccacact ttctggtggt gaagcccagc
     3181 gcgtgaagct ggcgcgtgag ctgtcaaaac gcggcaccgg gcagacgctg tatattctcg
     3241 acgagccgac caccggtctg cacttcgccg atattcagca actcctcgac gtgctgcata
     3301 aactgcgcga tcagggcaat accattgtgg tgattgagca caatctcgac gtgatcaaaa
     3361 ccgctgactg gattgtcgac ctgggaccag aaggcggcag tggcggcggc gaaatcctcg
     3421 tctccggtac gccagaaacc gtcgcggagt gcgaagcttc gcatacggca cgcttcctca
     3481 agccgatgct gtaatcgtta aggccgcttt ctgagcggcc ttttcctttc agagttgtac
     3541 cagcaattta cgcttttctt ccggtagtaa attcactgcc tgctgataag acgcatccac
     3601 cagataatag atttgcgaat ccggcagcga accgtcgaga tacacggtgc tccagtgcgc
     3661 tttattcaga tggcggctag gacgcacatc gctgtgctgc tgacgtagca gctccgccag
     3721 ctccgggctg gttttcagcg aaacagctgg gcgattttct acttctttca ccatcgcaaa
     3781 cagtacatct tccactttga tctgcgtcgc tttccagtcg ttatgcacgc tctgttctgc
     3841 gcttggtttt gccatgcaat attgtagcaa ctccgaaatg gtcatttttt actccccttg
     3901 tagtgtcgcg atgatgcgac gcgatccgcc gtggatgcga tgttccccca gccagatgcc
     3961 ttgccaagtg ccggtctgaa tacgcccttt atgtaccggc aatacaagcg atgttcccag
     4021 cattgaggat ttgatatgag aaggcatatc gtctgctccc tcatagtcat gctcataatt
     4081 tccgttgtcg ggaacggtgc ggaggaaaaa acactccatg tcgtggcgta cggtgggatc
     4141 gcagttctca ttaagtgtca gagaggcgga ggtatgttgc agcaacagat gcagtaagcc
     4201 gatgttaacg cgcggcatat cagccagctg attcagaatt tcatccgtta ccagatgaaa
     4261 cccacgagat ttggcgctaa gcgtgagcgt cttttgatac cacatgtgct gctccttgat
     4321 aaaactctct taatcagttt gcagcaagac agcgaaagga taaaggtgtg attaaaaaaa
     4381 cagcattcgg gagagcatca ctctctcccg ctctgtcagt attctgaatt gacgatcacc
     4441 tcttcaccaa acgcacccgc ttgtggcaag ggtttgtagg tagagttgga ggcgcgcaga
     4501 atgcggatac cacgagcgcc gacatcgcgt gcggcggtaa tatcgttatc ggaatcgcca
     4561 taaaaaattc ggatattttt atcctgcagc cattgcgatt ttgtattttg ccctggttta
     4621 tcgcccgcaa agattaccgg attcatgttg gtggcaggaa tatgaaaatt atccgccagc
     4681 gtttttgaaa ccgtttctgt tttcgtcggg ctacgaccag tcacaaagaa gatcgcgtca
     4741 ccgcggcgta catgcatatc aatcagctgg cgagcgacct cttttggaat gctgaattca
     4801 tcccagccat tattcatttt ttcccagaac acaggatttt tcagataatc ttcgctttct
     4861 ggcgagaagg tttttttgcc gcgccagaac cccggactgg aaaaaagtac cgtgtcatcg
     4921 atatcaaacc ccaccgccat tggcggacgc cctgcgaggc tattttcaat ttgtgcgacc
     4981 gaaacccaat gaattggtgc ctgttcagca agcctggcaa cgttagtccc agggttaagc
     5041 ggtgaaggag atgaggccag ggcaacagcg gaactgttta gagcgaacaa taagcaaacg
     5101 gcactgattg cctgtgtgat cttgcgcata tttttcccta aatagtcagt atgttgaaac
     5161 tttttattgt gagatttgtt gcaaaaacca tctgaccata acgccagctg ccggggaaag
     5221 gaaggattat ctgcggtttt tgtgatgaaa agcaggatga ttatatttaa cccagtaaat
     5281 acgttcctgt ctttttttgt ataaaaaatg tttattactc gatgtaagga tacattaatt
     5341 ttacgtttat tatgatgaat cctcggcaaa tgagcgaaat ttgatagaac atttatctgc
     5401 tgtacgattt aatgaactta atgatacagc attacaaaaa caatcgaagt ttataaagat
     5461 gatttctgat tgaccagccc cctaccctac aatggtgatt aattttatta ctcgcgatgg
     5521 agatgatgat atgaatatat catatgtgaa tagcaacaaa acgacatctt tacctgttga
     5581 gctagatgca ctaaataata aagatatctc ttatgccaaa gatttctttt tatacataga
     5641 aacacagtta aaaattgcaa aagatttttg tagacctgga gaagaagtat caagttctat
     5701 tgcaagtaaa gtttttcacg catttattga tttagttaac aaaatcaggg gtaagaaaga
     5761 ctttatgtat atttgcacgc tttgctgttt tgccgaggag gttaaaggtg attattctca
     5821 ttacaggacc tttttatttg atattggtaa tcaatataag gttaaactta cacaaagcgg
     5881 aaaaaagagt tctctttaac attagaattt aacgatacta taattgaatc tcagaaagtc
     5941 acaggcaata aggcaaagca tattcttgag gatatagaaa tattctatcg taacaaaccc
     6001 gatacttatt attaaattta ataatggtta gatgtgcaga aattattact ccacacgaaa
     6061 aaatagggaa aggaattcca gataattggg ataaacttaa caataaaaga actgacaacg
     6121 ttttattatc tctcacgtag aacaacggca tcaaaaaaat catctacaaa gaaatagtga
     6181 aagcgacgac agtttaattt cactgcaggc tgggtaactc cagcctgctt tcctgcatta
     6241 catcaccgca gcaaacgcct ttgccacacg ttgtacattt gccgtattta acccggcgac
     6301 acacatgcga ccgctggcga tgagatagac accaaattct tcacgtagtc ggtcaacctg
     6361 agcggcactt aaaccggtat aactgaacat gccgcgctga ttaagcagat aatcgaaatt
     6421 gcgttctggc atctctgtgc ttaatacctt caccaattcc tgacgcattg ccagaatgcg
     6481 agtacgcatc tcttctactt ccgccagcca gctggctttc aatgcctcgt cattcagcac
     6541 tgcagccacc acctgcgcac caaaattcgg cgggctggag tagttgcggc gaactgttgc
     6601 tttcaattgc cccagtacgc ggccagcggc ttcggcatct tcacacagaa cagaaagtcc
     6661 gccgacgcgc tcgccgtaaa gggagaaaat tttcgagaac gaattgctca ccagagcggg
     6721 taatccagca ctggcaatgg cgcgaatagc gtaggcatcc tcttccatac cggcactaaa
     6781 tccttgatag gcaatatcga ggaatgggat aagctcgcgg gctttgagaa tttcaatcac
     6841 cgcatcccac tgatcattag tgagatcggc acccgttggg ttgtggcaac atggatgcag
     6901 caacacaata ctgcgggcag gtaatgtttt cagcgtcgcc aacaggtcat taaagcgcac
     6961 gctgttagtc acttcgtcat accaggggta agtactcact tcgaatccag ccccggcgaa
     7021 tattgctacg tggttttccc aagtaggatc gctgacccag acgcctgatt ccgggaagta
     7081 gcgtttcagg aaatccgcgc ccactttcaa tgcccctgag ccgccaaggg tttgaatggt
     7141 tgctacgcgc tgttgttgca gtaccggatg gtccgcacca aacagcagcg gcgcaatggc
     7201 atggcgatag ctgttaagcc cttccatcgg taaataaagc gaagcgccat ggggctgcgc
     7261 attcaggcgc gcttccgcct ccgccacggc tttcaattgt ggaataattc cgtcttcgtt
     7321 gtagtacaga ccgatactta aattcacttt gtcgctgcga gggtcttctt taaaacgctc
     7381 cataagcgta agaatcgggt cgccagcgta ggcgtcaact ttttgaaaca cgcgatggtt
     7441 ctccaggttt acgggcaggt ggttaaaaca caataaaccg gaagaaggcg aagatcgagt
     7501 ggatgttcag gagcgaacgg caattagcaa cagagtgaga ctaatgataa acgtacatcc
     7561 gccagaggca cgacctttat aagagcatga aagaacatca acttattgaa tttttaggat
     7621 tttattggcc ggataaggca ttcacgccgc atccggcaca gacaatcaaa tattacagaa
     7681 cgattaatcc acgtatttca tcgcgaccct tgaagtcagg cgcgtaataa gttcgtaagc
     7741 gcttactttc gtcatttcag cgatacgttc tacgggcaaa ccttcgcccc ataaaatgac
     7801 cggatccccg gctttgtcct gcgcctgtgg acctaagtct acgcagatca tatccatcgc
     7861 cacgcgcccg acaatcggta cttcgcgacc gttcaccagc actggcgtac cggacggcgc
     7921 ggcgcgcgga taaccatcgc catagcccat cgcgactacg ccaagacggg tatcacgttc
     7981 gcttacccag gttccaccat aaccaacagg ctctccggct ttatgctcac gcacggcaat
     8041 caggctggag gttagtgaca tcactggctg acagccaaaa tcggcaccgg tggagcgatc
     8101 ttccagcggc gagacgccat aaagaatgat gcccgggcgc acccagtcaa aatgcgactg
     8161 tggccacagc agaatgccac ccgacgcggc aatggaacgt tgaccaggtt tgccttcgca
     8221 aaaggtatta aagatagcga gttgtttctc ggttgcgcca cattttggtt catccgcgcg
     8281 cgcaaaatgg ctgacgatat tcaccggctg acgaacgttt ttgcactggg tcaggcgatg
     8341 ataaaacgcc tcagcctgtt ccggccttac gcccagacgg tgcataccgg tatcgagttt
     8401 catccagacg gtaaccggct cgtccaagct agcctcttcc agcgcagcca gctgttcttc
     8461 gttatgcacg gcggtatgaa aatgttgcgc agaaatcgtc ggcagatctc tggcatcaaa
     8521 aaagccttcg agtaacagta caggtttggt gattcccccc gcacgcagtc gcagagcttc
     8581 ttcgagacgg gctacgccaa aggcgtcagc atcggggagc gttcgcgcgg tctcaagaag
     8641 accgtgacca taagcgttcg ctttcaccac cgcaaccatt ttactggcag gtgccagttc
     8701 acgaagacgt tgcaggttgt gtcgcagagc gcggcggtta atcacaacag ttgccgcttg
     8761 catttgtgtt ccttgataat tgtttgcttt aattacctaa ttcataaaat aattattatt
     8821 cgtcgtcgta ctgcggcccc gcatagttgt cgaagcgcga ccattgaccg ttaaaggtca
     8881 ggcgtaccgt cccgattggg ccgttacgtt gtttaccgat aataatttcc gcgatgcctt
     8941 ttaaatcact gttttcgtga tacacctcat cacgatagat aaacatgatc aagtccgcat
     9001 cctgctcgat agagccagat tcacgcaggt cggagttgac cggacgtttg tcggcacgtt
     9061 gttccagaga acggttcaac tgggacagcg ccaccaccgg cacgttcagt tctttcgcca
     9121 gtgctttcag cgagcgggag atttctgcaa tttccagcgt acggttatcg gaaagcgccg
     9181 gtacgcgcat cagttgcagg tagtcgatca tgataagccc gatgccgccg tgttcacggg
     9241 caatacggcg tgcgcgggaa cgcacttccg ttggcgtcag gccggaagag tcatcgatat
     9301 agatattgcg tttttcgagc aaaataccca tggtgccgga aatgcgcgcc cagtcttcat
     9361 catcgagctg cccggtacgg attttagtct ggtcaacgcg cgacagcgac gccagagaac
     9421 gcatcatgat ctgttctgat ggcatctcca gcgagaagat aagtactggt ttatcctgca
     9481 acattgccgc gttttcgacg aggttcatcg caaatgttgt tttacccatc gacggacgcg
     9541 cggcgacgat gatcaaatcc gacggctgca agccagcggt ttttttgttg agatcgtcat
     9601 aaccggtgtt taccccggta acgccatcgt gtggctgctg aaacaactgc tcaatacgcg
     9661 ccacggttgc gtcgagcaca tcggcgatgt tcttcggccc ttcgtctttg tttgcacgac
     9721 tttcggcaat tttaaagacg cgggattcag caaggtccag cagatcttcg ctggtacgcc
     9781 cctgcggatc aaaaccggct tcagcaatct cattcgcaac cgagatcatc tcacggacaa
     9841 cggcacgttc acgcacgatg tccgcataag cactgatgtt cgccgcactt ggcgtatttt
     9901 ttgacagctc tgccagataa gcaaaaccac cgacgctatc gagttgcccc tggcgttcca
     9961 acgattccgc aagggtaatc agatcgatag gactaccgct ttcctgcaaa cgcgccattt
    10021 cagtaaagat atgacggtgt gggcgggtgt aaaaatcgtc tgctaccaca cgctcggcta
    10081 catcatccca gcgttcgtta tccagcatta aaccgcccaa caccgactgc tccgcttcga
    10141 tcgagtgcgg aggcactttc agcccggcaa cttgtggatc gcgttcgcgg ggttcagcct
    10201 gctgtttgtt gaagggttta tttcctgcca tagtgaatgg agttaccgaa ataaagaatg
    10261 ggtcgaaact ttaccatatg aagcagaccc tgacgatacg ttctggagga cacatggcaa
    10321 cacgaattga atttcacaag cacggtggcc cggaagtact tcaagccgta gagttcactc
    10381 ctgccgatcc ggcggagaat gaaatccagg tcgaaaataa agccatcggc atcaatttta
    10441 tcgacacgta tatccgcagc ggcctttacc cgccgccatc gctacccagc ggattaggta
    10501 ccgaagcggc aggcatcgtg agtaaagtcg gcagtggtgt aaagcatatt aaggctggcg
    10561 atcatgtagt ctatgcgcag tcggcgttag gcgcttacag ctctgtgcat aacattaatg
    10621 cggataaagc ggcgattctg cctgcggcaa tttcttttga gcaagctgcg gcatccttcc
    10681 tgaaaggctt aacggtttat tatctgctgc gcaaaaccta tgaaattaaa cccgatgaac
    10741 agttcctgtt ccacgcagcg gctggcggca ttggcttaat tgcctgccag tgggcaaaag
    10801 ccctgggcgc gaaactcatc ggcaccgtag gaaccgcgca aaaagcgcag agcgcgctaa
    10861 aagccggcgc gtggcaggtt attaactatc gtgaagagaa tctggtcgag cgattgaaag
    10921 agatcaccgg cggtaagaaa gtgcgcgtag tgtacgattc cgtgggcaga gacacctggg
    10981 aacggtcgct ggattgcctg caacgccgcg gcttaatggt cagttttggc aactcatcag
    11041 gtgcggttac cggtgtgaac ttaggcattc tcaatcaaaa aggctcgttg tatgtgacac
    11101 gcccttccct gcaaggctat atcaccacgc gggaggaatt aaccgaggcc agtaatgaac
    11161 tgttctcttt gattgccagc ggtgtgatta aggtcgatgt cgccgagcag cagaaatatc
    11221 cgctgaagga tgcgcagcgt gcgcatgaga ttctggaaag ccgggcgacg caaggttcca
    11281 gcctgctgat tccataaaag aaatagggct tccacctggg aagccctttc tttttatagt
    11341 tcggctgtat gtagggtaca gcacgatgaa tctgttagag gcgcaatagt gacagatttg
    11401 attatcaatt cctattttgt tctaaggata aaaccttagg ttgtgatcat ccgcacaatc
    11461 ccttagtaac gccagcggtc ataacgctga tatttcggca tttttggtgc tttaatcgcc
    11521 ttaataaccc acaccaccgc aatcgccagt agtaaccacg gcagcaactt aatcatcaat
    11581 gccagcatac cgccgaggaa cataatggcc gtcgccacaa ccagcgcggc gataatgccc
    11641 agcaacgaaa cgccggtgac catcagcatg acaaaaaagc caatcacaaa aagtagttcc
    11701 agcatgatgc tctcccaaat atgaaatctc ttgctggcat tacaagaatc atgccaaaaa
    11761 taatctattg atttaacagc aaaacgcccc gcgacggtgc gcagggcgtg gtgaatttga
    11821 ctactttttg gtgaaaagtt aacgcttatc cgccaccagt ttgagcgcgt gttccagcac
    11881 gttaatgtca gcacccgctt tatgggcatt ttcacttaaa taacgccgcc actgccgcgc
    11941 gccaggaata ccctggaaca aacccagcat atgccggata atatggccga gatacgtccc
    12001 ctggctgagt tcatgctcaa tgtacggata catggcacgc actactgcca ccggatcggc
    12061 atcggtatcc gaggaaccaa agatctcccg gtctaccgcc gccagaatac ccggattctg
    12121 atacgcctcg cgcccgacca tcacgccatc catatgttgc aaatgcgctt tagcttcttc
    12181 cagcgacttg ataccaccgt taatcgacat tgtcagatgc gggaagtcac gcttcagttg
    12241 atacacacgc ggataatcga gcggcgggat ttcacggttt tctttcgggc ttaaccccga
    12301 aagccaggct ttacgtgcgt ggatgatgaa catctcacac tcgcctttgc cggaaacggt
    12361 attgatgaaa tcgcagagaa attcatagct gtcctggtcg tcgatgccaa tacgcgtttt
    12421 caccgtcacc ggaatcgaca ccacatcgcg catcgctttc acgcagtcgg caaccagctg
    12481 cgcattaccc atcagacacg caccaaacat gccgttctgc acccggtcag acgggcagcc
    12541 gacattcagg ttgatctcat catatccgcg cgcttctgcc agctttgcac actgcgccag
    12601 cgccgccgga tcgctaccgc ccagttgcaa cgctaccgga tgttcttctt cactgtacgc
    12661 cagataatca cctttaccgt gaattatcgc ccctgtggtc accatttcgg tataaagcaa
    12721 cgtattgcgg gaaagcagac gcaagaaata gcggcaatgt ctgtccgtcc agtcgagcat
    12781 tggtgcaaca ctaaaccgac cactccagtg aacgtcagtt ttttcaggca ttgcgctggt
    12841 ttggttgatt ttttgcattt cagaattacc gtgcattttc aaatgtagag attattttat
    12901 cgatatatca tggggttatg ttattcagct tcactgttca ggaggctcaa tagcggggta
    12961 ctataccata acaacaggaa gcgcctgtct cattgcaaaa gaaaattgag atcctctcaa
    13021 ggcatgaagc tctcacgaag tgatggaaat aatcttatta gccgttaact ttgttaaggc
    13081 caatgataaa caatccaggt tcgacgataa ataaaaaatc acacattaaa ctctggtgat
    13141 atatctccct gctaatagca ttgatagaga aaaaagaacc caataaatat tgggtccttt
    13201 tatataatgc cttccatact atcgaagaac ttcacatatt atttctccga tttaaccccg
    13261 aacaagtcat aaattaattt agaagtgtct gtaagtattt taatctcttc ctttgaggtt
    13321 gggtcaaacg acttcgcgaa gctaattaat tgtggtgcag catctcgcat tttgcttaaa
    13381 atatcaggat cgagcgttcc ttcattcacc agatgtgaca tcttatccag atagccatca
    13441 aaattcattt ctcccccatc tgccagacgc ttcatctctc ccaggtactt cttcatatca
    13501 cgttgatata atttttcaaa ctgagctttc aggtagctct cattattttc attaatatac
    13561 actgtttctg ataaatctgc gtaggcacta gaatatgaaa gtgaaattaa cagcgaagca
    13621 aataatttta agctgttgtc atcatgttcc tggcaggcat taacaaacgt taaaaaacca
    13681 gaacctattg cttgaaaatg tatattcgct aaaggttcat tatccttaga ttcctcataa
    13741 aatagctgaa ccgtggaggg aaggccattc ttagtttcta catcaaaagt acattgttca
    13801 atgggcattg catctttatt atccccagga ttacatatac tattaatcgc gaaatgaaaa
    13861 atagcccggt caacaactga tgcaataaag tcacgatcgt ttaaatcctc atcagtgcaa
    13921 tcgtcagtat acttattgac ccacattttg agatcattta tgtgctcaat taaaaacaca
    13981 tttttttacg tgcatgccat aaattatgcg tgattcgttg aataaacaaa tctatttttg
    14041 caataattat atcccgatga cgcagcgctg cataatcaat agatttacca tatatattat
    14101 ttaatttacc tttgattgaa cctctattat ccaatttatc tttggttgca tctttatttt
    14161 cgaattgatt tgaattgagt gttttattaa agtatccagt tgcaccgggt attatcttca
    14221 acatgattca tctacctttt ttgtcgtacc gtgtcctcct tataattctt ttgccatctg
    14281 gtcactatca aaaatcgctc ataatttctg aactacaggt atgcgtttaa acccagttaa
    14341 ataaatagcg agttaacttg aagcaattta tttaaaaatg aattattaat tattatcgtg
    14401 ttgataatgg tacgataagg caacacaaca ccttacctac aaaacagaaa atcaacatta
    14461 cggcaacaat aagggttctc ggtgtttttc ctggaacatg gtaaagtaag gacattctta
    14521 acccccactt tgaggtgccc gatggaaaag accacaacgc aggagttatt agcgcaggct
    14581 gaaaaaatct gcgcgcagcg taatgtgcgc ctgaccccac agcgcctgga agtgttgcgc
    14641 ctgatgagtc tgcaggatgg cgctatcagc gcttatgatc tgcttgattt actgcgcgaa
    14701 gctgaaccgc aagccaagcc gccaacggtt tatcgcgcgc tggattttct gcttgagcaa
    14761 ggttttgtgc ataaggtgga atccaccaac agttatgtgc tctgtcatct gttcgaccag
    14821 cccacgcata cgtcagccat gtttatttgc gatcgctgcg gcgcggtgaa agaagagtgt
    14881 gcagaaggcg tggaagacat tatgcatacg ctggcggcaa aaatggggtt tgccctgcgg
    14941 cataatgtga ttgaagcaca tgggctctgt gcggcatgcg tggaagtgga agcgtgtcgt
    15001 catcctgaac agtgccagca tgatcactct gtgcaggtga aaaagaaacc gcgttaagag
    15061 ggcgtacatc cttgtacacg tcgggcagga gggattaatt accagcgata ttcattgcgg
    15121 gtttcccaat ccacgacctc tttttctgcc tgatctttct gataaccata acgttcctgg
    15181 attttaccga ccagttgatc acgtttacct tcaatgatcg tcatatcatc atcggtcagt
    15241 ttgccccatt gctctttcac tttaccttta aactgtttcc agttaccgcc ggcttcatct
    15301 ttattcataa tcaagacctc atcgttaggt tgtgaatgag agtacgttca cttttcttct
    15361 gaacgtgaga ttaagtgtag tcagcaattt ggcttaggat aatttattca gaatttttaa
    15421 ccgtcacgtt gcggcaaacc aggtaccgtt gcgccagtga cgccgccaga tagccgccag
    15481 agaaagcccg cgcaacgcca gaaagacggt taatgccagc cacaaaccat gattacccag
    15541 ccacggcagc gtaaggagcg tcagcgcaaa acctgcggcg gccaccgcca tactgttacg
    15601 catttcggcg gcacgcgttg cgcctataaa catgccgtcc agcagataac accagacgcc
    15661 aaccaacggc aaaatcacct gccagataag atagcggtca gccagctgct gaatctgggt
    15721 taacgacgtc agcaacgcaa tgatgtgttc ccctgccagc aaataaacca ccgaaaatag
    15781 taacgctaca atccccgact ggcggcacgc tgcccgccat acatccagca actggctacc
    15841 gtcgcgcgca ccgtatgcct gaccggagtg cgcttcaacc gcgtaggcaa aaccatcaag
    15901 cgcataggcg gtaaaggtga gtagcgtcat cagaaccgcg ttaacagcga taatgtcact
    15961 ccccagtcgc gcaccaagta cggtgatcgc gccgaaacag agttgcaaca acagcgaacg
    16021 cagcatgata tcgcggttaa gcgccagcaa gcgacggaag tttcctcgcc aggcagtttt
    16081 cagcatttcg ccggaaattc cgcgtagttt gaggatttta cgcaccatta gcagaccaat
    16141 cagcaatgtt gcatattccg caataaccgt cgccagcgcc gcgccctgca cgttcatatg
    16201 cagccccatc accagccaga catccagcac aatgttgagg atattgccga ccactaacaa
    16261 aattactggc gcacgggcat attgcacgcc gagtaaccaa ccgagtaata ccagattcgc
    16321 cagcgacgcc ggagcgctta accagcggat ttcaagaaag cgccgcgcct gttccaggac
    16381 cgcttcacta ccgccaacaa tatgcagcgc cagatcgata atcggcgtac gcaacagcgc
    16441 aattaacgcc ccggccccca acgccaacag caacggttgc accagcgcac gggctaatgc
    16501 ctgaggattt ttggcaccat aagcctgcgc agtcagcccg gtggtgctca tgcgtaaaaa
    16561 cagcaacagc ataaagagaa agctggtcgc cgttgcacca accgccacgc cgcccaaata
    16621 aaccggacta tcaagatgac caattaccgc cgtatcgacc agtcccagca acggaacggt
    16681 gatattggag aaaatcatgg gtaaggcgag atgccagagt gctttatcag atgaagtgag
    16741 gaatgccatg cagacaagcc tgatgaagag agatgaaaaa caaaccgcga cgccaagcgg
    16801 catcgcggtc tcagagatat gttacagcca gtcgccgttg cgaataaccc caaccgccag
    16861 cccttcaatg gtgaagctct gctgacgaag gtcaacgaca attggtttaa actcgctatt
    16921 ttctggcaac agttcgactt tattgccctg ttttttcagg cgcttaacgg taacttcgtc
    16981 atcaatacgt gcgacaacga cctgaccgtt acgtacatcc tgagttttat gcactgccag
    17041 caagtcgcca tccataatgc cgatatcttt catcgacatc ccgctgacgc gcagcaggaa
    17101 atcagcattc ggcttgaaca aggaaggatc gacctgataa tgaccttcaa tatgctgttg
    17161 cgccagaagc ggttcaccgg cagccacacg acctaccagc ggcaaccctt cttcctcttc
    17221 ctgcaacaga cgaatcccgc gtgatgcgcc ggaaacaatt tcaataacgc ctttgcgtgc
    17281 cagcgccttc agatgttctt cagccgcgtt tggggaacgg aaccccaaac gctgcgcgat
    17341 ttccgcacgc gtcggcggca tacctgtctg gctgatgtga tcacggatga gatcaaacac
    17401 ctcttgttgc ctggccgtta acgctttcat tccgccccct gggtgtatat acagttatac
    17461 tgtgagtata tacagcaaaa ggcgattttg gaaccataaa ctgcacaata aaccagagat
    17521 ttatcgaaat ctggaagggt tatccaaaat gcgaccataa caggatgcac caggtaatca
    17581 cggcgacgat aatggcaatc agcaccgcgg cggatcccat atcttttgcg cgtccggaaa
    17641 gctcatggta ttcagagcca attcggtcaa ccactgcttc gatggcgcta ttgaggattt
    17701 ccacaatcat caccagcatc acggagctga taagcagcac gcgggtaatt gcgtccacat
    17761 ccagccagca ggcgatgacc accgccaaca ataccgctac gccttcctga cggaatgccg
    17821 cttcgttgat ccatgcagcg cgtaaacctt tccaggaata gccagcagct ttgataattc
    17881 gggtgaatcc agttgtatta ttggccattg aaagaacctt tttacattat gagcgtcaat
    17941 atcagtgtac cgttaaatcc gatgattcgg gtttgcgtaa cgccgcgcga aacatgagcg
    18001 gataccacag aatttcccat gactttctgc tatccttgcc gcgcatttgc attattaacc
    18061 agaggcttta catcgtttat gtccggctgg ccacgaattt actacaaatt actgaattta
    18121 ccattaagca tcctggtaaa aagcaagtct attccggcag atcctgcccc ggaactgggg
    18181 ctggatacct ctcgtccaat tatgtacgtt ttaccgtaca actcgaaagc agatttgctg
    18241 acgttgcgcg cccagtgtct ggcacatgat ttgcctgacc cgttagagcc gctggaaatc
    18301 gacggcacgc tactgccgcg ctatgtgttc attcacggcg ggccgcgtgt attcacctat
    18361 tacacgccga aagaagagtc tattaagctt ttccacgact atctcgattt gcaccgtagc
    18421 aacccaaatc tggatgtgca gatggtgcca gtatcggtga tgtttggtcg cgcgccgggg
    18481 cgtgaaaaag gcgaagtgaa cccgccgctg cgtatgctta acggcgtaca gaaatttttc
    18541 gctgtactgt ggctcggtcg cgacagtttt gtgcgtttct cgccgtcagt gtcgctgcgc
    18601 cgtatggcgg atgaacacgg cacggataaa actatcgctc agaaactggc gcgcgtggcg
    18661 cgtatgcact ttgcccgtca acgtctggct gccgtaggcc cacgtctgcc tgctcgtcag
    18721 gatctgttta ataagctgct cgcctcccgc gccattgcca aagcggtaga agatgaagcg
    18781 cgcagcaaaa aaatctccca tgaaaaagcg cagcagaacg cgattgcgct gatggaagag
    18841 attgcggcga atttctctta cgagatgatc cgcctgactg accgcattct gggcttcacc
    18901 tggaaccgat tttatcaggg catcaacgtc cataacgccg agcgcgttcg ccagctggcc
    18961 cacgacggtc atgagctggt gtatgtgcct tgccaccgca gtcacatgga ctacctgcta
    19021 ctttcttacg tgctgtacca ccaggggctg gtgccgccgc atatcgccgc cgggatcaac
    19081 ctgaacttct ggccggccgg gccgattttc cgccgtctgg gggcgttctt tattcgccgt
    19141 acttttaaag gcaataaact ttattccacc gttttccgtg agtatctcgg cgaactgttc
    19201 agccgtggtt attccgtcga gtacttcgtg gaaggcggtc gttcccgcac ggggcgtttg
    19261 ctggatccga aaaccggtac gctgtcgatg accattcagg cgatgctgcg tggcggcacg
    19321 cgtccgatta cgctgattcc gatctatatc ggttatgagc acgtcatgga agtgggtact
    19381 tacgccaaag aactgcgcgg cgcgacgaaa gagaaagaga gcctgccgca gatgctgcgc
    19441 ggtttaagca agctgcgtaa tctcggtcag ggttacgtca acttcggtga accaatgccg
    19501 ttgatgacct accttaacca gcacgtacca gactggcgtg aatctatcga tcccatcgaa
    19561 gcggtgcgtc cggcctggtt aacgccgacg gtcaataata ttgctgccga tctgatggta
    19621 cgcattaaca acgcaggcgc ggcaaacgcc atgaacctgt gctgtactgc gctactggca
    19681 tcacgtcagc gctcactcac ccgcgagcag ttaaccgagc aactcaactg ctacctggat
    19741 ctgatgcgca acgtacctta ctccacggac tctaccgttc cttcagccag cgccagcgag
    19801 cttatcgatc acgcgctgca aatgaacaag tttgaagtcg agaaagacac tatcggcgac
    19861 atcatcattc tgccacgcga gcaagcggtg ctgatgacct actatcgcaa caacattgcg
    19921 catatgttgg tgctgccttc gctgatggcg gcaattgtca cccagcatcg ccacatctcc
    19981 cgcgacgtat tgatggagca cgtcaatgtg ctttacccaa tgctgaaagc ggagctgttc
    20041 ctgcgctggg atcgcgacga gttgccggac gttattgatg cgctggcaaa tgagatgcaa
    20101 cgtcaggggc tgattaccct gcaagatgat gagttgcata tcaacccggc gcattctcgc
    20161 accctacagc tgctggccgc aggcgcgcgc gaaacgctgc aacgttatgc catcaccttc
    20221 tggttgttga gtgccaaccc gtcgatcaac cgcggtacgc tggagaaaga gagccgcacc
    20281 gttgcgcaac gtctctccgt gctgcacggc atcaacgcgc cggagttctt cgacaaggcg
    20341 gtgttcagtt ctctggtgct gacgctgcgt gatgaagggt atatcagcga tagcggcgat
    20401 gccgaaccag cagaaacgat gaaggtttat cagttgctgg cggagttgat tacatcagac
    20461 gtgcgtttga cgattgagag tgcgacgcag ggcgaagggt aatcagagag aattgccgga
    20521 tgcggcgaaa acgccttatc cggcctacca tgacctgcaa attcaataaa ttgcgattca
    20581 ccgggtaggc ctgagaagcg caacgcatca ggcaacccag aagaaagccg gatgatcatc
    20641 cggctttttt acatcatcag aaatgccagt aactcattgc cagtcctaaa aacagtacca
    20701 gaccaacata gttattattc ataaatgctt taaagcaggc ttcacgctcg cggttggcaa
    20761 tcagtttttg ttgataaaca aacagcgcgc cagccaccac aattgaccag taatatcccc
    20821 agcctaagcc atttaactca ccgatgatcg ccatcagtgc cagtacgcca atctgcaaaa
    20881 taccaataat caatttatcg tattggccga acaggattgc cgtggattta atgccaatct
    20941 tcacatcatc atcgcggtca accatcgcat actgcgtgtc gtaagccacc gcccagagaa
    21001 tattggcgag gaacattaac cagcaactca atggcaccga ctcactcaca gcggcaaaag
    21061 ccattggaat cgaccagcca aacgccgcgc ccagcaccac ttgcgggaga tgggtatacc
    21121 gcttcataaa cgggtacacc cacgccaatg ccagagcggc aatcgacaac agaatggtca
    21181 tcgtattcag cgtcagaacc agtaaaaacg aaatcagtac cagcactaca aacagcgcgc
    21241 gcgcctcttt ctctgttacc gcgccgctgg gaagtggtcg gttcgccgtg cgcttaacat
    21301 gaccatcaaa cttgcgatca gcataatcat tcaccacgca accagcagcg cgcatcagcc
    21361 agacacccgc gacaaacacc gccatgatcc agagctgagg aacgcccggt gtcgccaccc
    21421 acaacgccca taatgttggc cagagcagca gtaacgcgcc aattggctta tccgtacgca
    21481 ttaagcgatg aaacgccagc agcttattct gcgtcagact ccactccata tttttttcct
    21541 cttagtacaa cggtgacgcc ggtaaaaaca gttctgttag caacagcggt ttaccgctta
    21601 atcgcaggcg ggaacgtcgc ccccacagtc cggcatcacg gcctatctcg ataaaatccc
    21661 gggttagtgt cgatgatgtg aacagatagc gtcctaacgg cgttttaccc aatttttgta
    21721 acgctagttc cggcccgctt aatgttgata caggaacgac ggtacgaccg gcaagccacg
    21781 gttcaccatc ggcacataac aaaatttcac gtaaccagta acgagactct ttcggcagca
    21841 gcggcagttc ttcggggatt tcattctgct cgacaaaccc ctcgcggatc atcgtcacac
    21901 tcaccgtttt tccctgctgt tcaaaacgtt ttgtcatgga atcctccagc aacagccagt
    21961 cgagcagttg cggatccagg gcagggatct ctttaaaata gcgcagcgca cgcagttgcg
    22021 ttaacgcggg gtgtgacatg ccgaactctc cgttacataa cgtaaaggca ttgtatcgca
    22081 gaaaagtgtt tacgggagat gtaaagcaaa ttaagtgatc taatgcgcaa caaaaacgca
    22141 acaatcgcat gaagtggtaa aacgctgttg aaccttacgc tttgccgatg cccgtgctgg
    22201 cgagggcaac cataggggca tcaccgccca tgatgctagg aacggtgatg ttttccttta
    22261 cttaaatacc tttattcttt atacgagtag ctcgccaact cgcagtaaaa attgggaaaa
    22321 gcgcatattt accatgagat ttggagctga aaagtgtttc ctgagtaaga ttaataggca
    22381 acatcttatg tctggattta tagatacacg tggaattcca attggtatct tttgtaggat
    22441 tcaacatatc atgtaagcgc tgggagctta ccaccataac aggcccatca tcgacagagg
    22501 agaacaggat aaatatatta tcgtttcgtg ttaatttatt agtgttaaag gttcttaact
    22561 tttcttcagt ttctataaat ataggtgaaa catcatcact acgaatccac tctttataaa
    22621 gataccaggc atttgctttg atcatatcat ccgttgatcg taaggcctga tcgataagtt
    22681 ggataaaaaa attgttattt ttatcaacca gccctttatt catattaaat ctgttaataa
    22741 aatggatcat ctgttgggta tcgaaattca ccttactgaa tcgatgttga cgcataaaat
    22801 tatcgaactt gtcatcttcc ccagaatgta tataactacg tataattgga tcttttatat
    22861 aaggacaatg gctaaatgtt ttcttcatat tctcaataaa gtttttatta acaatattga
    22921 gaatttttaa ttgcatcacc agttttactg ccagaacgga tttcaatatc attgcattat
    22981 ctggcaatgt atcaatcatt tttataatgt tattgatata accatcgaga tcttcattcc
    23041 caaatatatc tgaataatca atagttaatt ccaattgttc tgtaatgcaa tcttaaaaat
    23101 tccctgtcga cattcgcaca tgatcaaaca taacagctgt caaattcgat ttaataaatg
    23161 atatttcttt ggcagacaga aactctgctc tggtgaagtg cgaagcgtaa agataacaat
    23221 tatcgaagtt tgaatttcta atctgacatt cgcgaaaatt actatgacca agttgactct
    23281 gcgagaagtc aactgtatca agaatatttt ggctaaaatc aacacattcg ccactcgttt
    23341 tgagaaaaat cacattgtgt aaacgtcgac atttaaatat gacattatca actgttgcct
    23401 tagtaaaatt gcaattctca atttctttac attcgaaagt tacatgttgt agatttgatg
    23461 ataaaaaaga caccttagaa ataacgtcac ctttaaaatg acaatttgtt aaatcaaaat
    23521 cagacaggtc gatatacccc tgcgcatctt tatccatcag aaaattacaa gcgcattcta
    23581 ttttttcatt tctgtagaat tttacactta tagtatcatt actaattgag acttcacgta
    23641 acatatttat tgcatgattt ttttcgttcc gttgttttat atttaaaaat atatcactga
    23701 tataaataaa gtaatctctg tttaaacctg tcaccccatt gaaattacgt agtaaatcaa
    23761 gatctactat attatttcct tgcaccacat catgtttcat caaagagtta ttgccgccag
    23821 aatttgtgat atgacctatc ataaaacctc tgtaatacag aaataaaata acctggaaaa
    23881 gtagcctgag cgtccagaca ctaccggcga aaggaaaaga atctctgata atgcattgaa
    23941 ataatggaca ttcttagtga agaagaagtg aagtactata aaaaatcgct catatcccag
    24001 gaaggaagca taaaaaaagg tgcgccagga gacgcaccag ttgttgcaaa atcagcactg
    24061 tggggcgtaa ttaccccttg ccttttacac tgctgataaa ggtggaacgg gcagatgtcg
    24121 atcccaggcg ttcagcttca tcaagcagtt ttaacgcctt atcaacatca cctttcgcga
    24181 cagcgttttt gatcgcggta ttaaaataac tttccgtgtc gttgagcatc ggctcgcttt
    24241 tcttcaccgg caccggagca ggtgcagcca cagctggtgc cgccgtgttg cctaccgtaa
    24301 ccggagctgg agcggaagaa ccaaatagcg gtcctaccaa cacgctggag ctggagttcg
    24361 ttttgacttt cagtttcagt aagccatcgc tggtatgacg agcaaccgga tcgggaatat
    24421 ccgggatcga gttaccgacg cctttggcat aggctttagc cgggtcgagc agttgggtcg
    24481 tctgctggag atctttttcc gtggtaaaga ccagaacata aagtttttgc tgccccaacg
    24541 ccggtgtcag gcgcataacg ccttccagcc gatctgcact catcacgcct agttcctggt
    24601 aggtgaaata actgctgggg aagaaggctg atggggtcat gttctgatca agaatcagca
    24661 cgttcggcgc aaaaacgctg gtttgtttgt tcacttcgct ggtcagcgtc agggtcagtt
    24721 cgccaatgtt tgccgggacg ctgtacgcag caaccggacc actgatgccg ggaacgttca
    24781 gttgttggcc gccggtcgcc agttgggtgg tctgggtttt agattgatcg accggtgtcc
    24841 aggtgagttg ttgcagcgcg gcagatggaa tggctggcgc gtcgctggtg ttttgcggta
    24901 cgtagttaac atcggcaagg ctaattccag gcgcgcttgc cagtaaccct gctgataaac
    24961 agagggcgat gagactttta ttcattttca ttgttttcac ctcaaaatct ggagctcagc
    25021 ggtagccagg caatagcgcg ctaaacccga taatcagagg ggctttcgcc cctttcagat
    25081 aatgacaacc tgtttttatg ccggatgcgg cgtaaacgcc ttatccggcc caggttttgc
    25141 tattaccacc agatttccat ctgggcaccg aaggtccact cgtcgctgtc gccacgaccg
    25201 aagctgccgc cgttgaaatc agcaggaacg gctttgccgt agttcgggtt aaccttgcta
    25261 tcgccgttgt agtcgtaacc ccatttctca tcccacttgg cgtaggttgc gaagacacga
    25321 atagccgggc gtgaccagat gctgtcgcca gcctgccatt gttgtgcgag ggtaattttg
    25381 tactgattgt tcttgtcgcc ggtgcgctgg gattcgacgt tgtcgtagcc gatttccatc
    25441 acggtgctca tgattggcgt ccacttgtac atcgggcgaa taccaacggt ccaccacttg
    25501 gtgccgttgt cgttatccca gttgatatcc tggtacatac ccacgtacat catgtcccag
    25561 ttgtcgccca tggagatcgc accgtggtcg aggatacgca gcatgtgacc gttgttgttg
    25621 atattgtagg caaatttttc gttatcaaac gcgacgccag aaccctgcga cagaccttta
    25681 ccctgcgagg tcatcgagtc agtagcgtac tgaacaacaa acttgttaaa gcccttcagg
    25741 acactctgag tatgttcagc agtgaataac cagccgtctt tcgatgcgcc atcaaccaga
    25801 cgatagttat cacgcaggtt ggcacgaccg tagtcgacac ccagttctaa tgtgccgccc
    25861 gggttgattt ccatctgcgc taaacgcaca tcgaaaacgt cgttcgcggt ttcgttggta
    25921 tagtcataaa tattgttgct ggcaaaagag gaagaaccac cagcttcaga ggagcgggtt
    25981 gctgccagag agagtttacc gaagccaaca tcgatgtttt ccagaccggc accaggacca
    26041 gaaatatccc agtagtagaa gtcgatcata tgaacgtcat gacgttggta gaagcgctta
    26101 cctgcccaga tggtggaacc tggcagccat tcgatcaggt tttttccctg cacgtttgct
    26161 tcacggaagg ccggatcggt agcttcccag tcattctgtt gcgcgacgga ataggccacg
    26221 ttagtgtcga aatagaagct cttatcgccc tctttccaca cttcctgacc caattttaat
    26281 tcagcataag tttcacattc gttgccaaga cggtatttac tttgagcacc ggtagtctgg
    26341 aaacactgtt gttcaccgcc gctacctgtc cagccaatac cggaacgtgc atagccgtgg
    26401 aaatcaacag ccattgcctg agcagacatt acgcccgctg cgacggcaac cgccagagga
    26461 agtttgcgca gagtaatcat cattctatct cctgagtcat tgcttttctt ttttcacatc
    26521 acctgtgaca ggctttgtgt gttttgtggg gtgcttaaac gcccggctcc ttatgcagtc
    26581 gacgacatgc agtgccatcc tcacggaaca gatggcaacg ctctggcggc aggccgatag
    26641 cgaatgtggc accttcttct accaacacca cgtcgttctg gcggtacacc aggttttgac
    26701 gaatggaagg gatctggata tagatttggg tttcgttgcc gagttgctcg acgacctgaa
    26761 cttcaccctc aaggatgacg tcagcgatat cactcggcag tagatgttcc gggcgaatac
    26821 ccagcgacat attggctcca acctggacat cacggctttc aacaggcagc cagacttgtt
    26881 gacgatttgg catcggcagc tccacctgca cttgatcgat tgcggtggcg gtcactttta
    26941 ccggcaggaa gttcatcttt ggcgaaccga taaatccggc gacaaaacgg tctgccggat
    27001 agtggtacag ttccagcggt ttcccaacct gtgccacgcg accggcgtcc agcaccacga
    27061 ttttgtcggc cagcgtcatc gcttcgacct gatcgtgggt gacgtaaatc attgtgcggc
    27121 ccaggcgttt atgcagacgg gagatttcga tacgcatttg cacacgcagt gcagcatcga
    27181 ggttggagag cggttcatcg agcaaaaata cgcttggctc ggccaccagc gtacggccaa
    27241 tcgccacgcg ctgacgctga ccaccggaga gcgctttcgg tttgcgatcc agcaaatgcg
    27301 ccagttgcag cacttccgcc acctggttga cgcgttggtt aatcacctct ttttttgcgc
    27361 cagccagttt caggccaaat gacatgtttt ctgctactga caggtgggga tagagcgcgt
    27421 aagactgaaa caccatacca acgccgcgtt ctgctggcgg agtgtcattc atccgtttct
    27481 caccgatgaa caggtcgccg ctggtgatcg tctcaagccc ggcaatcatg cgcagtaaag
    27541 tcgatttacc gcagccagac ggtccgacaa acaccacgaa ttcaccttca tggatatcga
    27601 gattgatatc tttcgatacc acgacctcgc cccaggcttt cgttacattt tgcagctgta
    27661 cgctcgccat gcccttctcc ctttgtaaca acctgtcatc gacagcaaca ttcatgatgg
    27721 gctgactatg cgtcatcagg agatggctta aatcctccac cccctggctt ttttatgggg
    27781 gaggaggcgg gaggatgaga acgcggcttc tgtgaactaa accgaggtca tgtaaggaat
    27841 ttcgtgatgt tgcttgcaaa aatcgtggcg attttatgtg cgcatcccca cattaccgcc
    27901 aattctgtaa cagagatcac acaaagcaac ggtggggcgt aggggcaagg aggatggaaa
    27961 gaggttgccg tataaagaaa ctagagtccg tttaggtgtt ttcacgagca cttcaccaac
    28021 aaggaccata gattatgaaa ataaaaacag gtgcacgcat cctcgcatta tccgcattaa
    28081 cgacgatgat gttttccgcc tcggctctcg ccaaaatcga agaaggtaaa ctggtaatct
    28141 ggattaacgg cgataaaggc tataacggtc tcgctgaagt cggtaagaaa ttcgagaaag
    28201 ataccggaat taaagtcacc gttgagcatc cggataaact ggaagagaaa ttccctcagg
    28261 ttgcggcaac tggcgatggc cctgacatta tcttctgggc gcacgaccgc tttggtggct
    28321 acgctcaatc tggcctgttg gctgaaatca ccccggacaa agcgttccag gacaagctgt
    28381 atccgtttac atgggatgcc gtacgttaca acggcaagct gattgcttac ccaatcgctg
    28441 ttgaagcgtt atcgctgatt tataacaaag acctgctgcc gaacccgcca aaaacctggg
    28501 aagagatccc ggcgctggat aaagaactga aagcgaaagg taagagcgcg ctgatgttca
    28561 acctgcaaga accgtacttc acctggccgc tgattgctgc tgacgggggt tatgcgttca
    28621 agtatgaaaa cggcaagtac gacattaaag acgtgggcgt ggataacgct ggcgcgaaag
    28681 cgggtctgac cttcctggtt gacctgatta aaaacaaaca catgaatgca gacaccgatt
    28741 actccatcgc agaagctgcc tttaataaag gcgaaacagc gatgaccatc aacggcccgt
    28801 gggcatggtc caacatcgac accagcaaag tgaattatgg tgtaacggta ctgccgacct
    28861 tcaagggtca accatctaaa ccgttcgttg gcgtgctgag caccggtatt aacgccgcca
    28921 gtccgaacaa agagctggcg aaagagttcc tcgaaaacta tctgctgact gatgaaggtc
    28981 tggaagcggt taataaagac aaaccgctgg gtgccgtagc gctgaagtct tacgaggaag
    29041 agttggcgaa agatccacgt attgccgcca ccatggaaaa cgcccagaaa ggtgaaatca
    29101 tgccgaacat cccgcagatg tccgctttct ggtatgccgt acgtactgcg gtgatcaacg
    29161 ccgccagcgg tcgtcagact gtcgatgaag ccctgaaaga cgcgcagact cgtatcacca
    29221 agtaatgctg tgaaatgccg gatgcggcgt gaacgccttg tccggcctac aaaactgaaa
    29281 cgcatgtagg cctgataaga cgcatcagtg tcgcatcagg cagttgttgt cggataaggc
    29341 gtgaaagcct tatccgtcct ggaatgagga agaaccccat ggatgtcatt aaaaagaaac
    29401 attggtggca aagcgacgcg ctgaaatggt cagtgctagg tctgctcggc ctgctggtgg
    29461 gttaccttgt tgttttaatg tacgcacaag gggaatacct gttcgccatt accacgctga
    29521 tattgagttc agcggggctg tatattttcg ccaatcgtaa agcctacgcc tggcgctatg
    29581 tttacccggg aatggctgga atgggattat tcgtcctctt ccctctggtc tgcaccatcg
    29641 ccattgcctt caccaactac agcagcacta accagctgac ttttgaacgt gcgcaggaag
    29701 tgttgttaga tcgctcctgg caagcaggca aaacctataa ctttggtctt tacccggcgg
    29761 gcgatgagtg gcaactggcg ctcagcgacg gcgaaaccgg caaaaattac ctctccgacg
    29821 cgtttaaatt tggcggcgag caaaaactgc aactgaaaga aacgaccgcc cagcccgaag
    29881 gcgaacgcgc gaatctgcgc gtgattaccc agaatcgtca ggcgctgagt gacattaccg
    29941 ccattctgcc ggatggcaac aaagtgatga tgagctccct gcgccagttt tctggcacgc
    30001 agccgctcta cacactcgac ggtgacggca cgttgacgaa taatcagagt ggcgtgaaat
    30061 atcgtccgaa taaccaaatt ggcttttacc agtccattac cgccgacggc aactgggggg
    30121 atgaaaagct aagccccggt tacaccgtga ccaccggctg gaaaaacttt acccgcgtct
    30181 ttaccgacga aggcattcag aaaccgttcc tcgccatttt cgtctggacc gtggtgttct
    30241 cgctgatcac tgtcttttta acggtggcgg tcggcatggt tctggcatgt ctggtgcagt
    30301 gggaagcgtt gcgcggcaaa gcggtctatc gcgtcctgct gattctgccc tacgcggtgc
    30361 catcgttcat ttcaatcttg attttcaaag gattgtttaa ccagagcttc ggtgaaatca
    30421 acatgatgtt gagcgcgctg tttggcgtga agcccgcctg gttcagcgat ccgaccaccg
    30481 ctcgcacgat gctgattatc gtcaatacct ggctgggtta tccgtacatg atgatcctct
    30541 gcatgggctt gctgaaagcg attccggacg atttgtatga agcctcagca atggatggcg
    30601 caggtccgtt ccagaacttc tttaagatta cgctgccgct gctgattaaa ccgctgacgc
    30661 cgctgatgat cgccagcttc gcctttaact ttaacaactt cgtgctgatt cagctgttaa
    30721 ccaacggcgg cccggatcgt cttggcacga ccacgccagc cggttatacc gacctgcttg
    30781 ttaactacac ctaccgtatc gcttttgaag gcggcggggg tcaggacttc ggtctggcgg
    30841 cagcaattgc cacgctgatc ttcctgctgg tgggtgcgct ggcgatagtg aacctgaaag
    30901 ccacgcgaat gaagtttgat taagggagat aacaaaaatg gcaatggtcc aaccgaaatc
    30961 gcaaaaagca cgtttgttta ttactcacct gctactgcta ctttttatcg cggcgattat
    31021 gttcccgctg ctgatggtcg tcgctatctc gctgcgtcag ggaaacttcg cgaccggtag
    31081 cctgatcccg gagcaaatct cctgggatca ctggaaactg gcgttaggtt ttagcgttga
    31141 acaggctgat ggtcgcatta cgccaccgcc attcccggta ctgctgtggc tgtggaactc
    31201 ggtaaaggtc gctgggattt ccgcgattgg cattgtggcg ctctccacca cctgcgccta
    31261 cgctttcgcc cgtatgcgct ttccaggcaa agcgacgctg ctgaaaggaa tgctgatttt
    31321 ccagatgttc ccggcagtac tttcactggt cgcgttgtat gcgttgtttg atcgtctggg
    31381 tgagtacatt ccattcattg gcctgaatac tcacggcggc gtaattttcg cgtatctggg
    31441 tgggattgcg ctgcatgtct ggaccatcaa aggctatttc gaaaccatcg acagttcgct
    31501 ggaagaagcg gctgcgctgg atggtgcgac accgtggcag gccttccgcc ttgtcctgtt
    31561 gccgctgtca gtaccgattc tggcggtggt attcatcctg tcgtttatcg ctgccattac
    31621 tgaagttccg gtcgcgtcgc tgttactgcg tgacgtaaac agctacaccc tggccgtggg
    31681 gatgcagcaa tacctcaacc cgcaaaacta cctgtggggt gactttgccg ccgctgccgt
    31741 gatgtctgca ttaccgatca ccatcgtctt cttgctggct caacgctggc tggtcaacgg
    31801 cctgacggca ggtggtgtga aaggttaaag atgttgttct gccaatgtta tgccgctgca
    31861 ccctcaactt acgttatccc aacttgtgac tattattcgg cgctccacgg agcgcctttt
    31921 tttctttcgt ctgcaatctg aatcgttcgc cggttaatat tcccatcata gaacttatta
    31981 tttttacgtt atttgttttc ccacttacga taattctctt tcgtgctctg agtcacggca
    32041 atagtattgt ttcatcaatt tttgataatt atcacaatta agatcacaga aaagacatta
    32101 cgtaaacgca ttgtaaaaaa tgataattgc cttaactgcc tgacaattcc aacatcaatg
    32161 cactgataaa agatcagaat ggtctaaggc aggtctgaat gaatacccag tataattcca
    32221 gttatatatt ttcgattacc ttagtcgcta cattaggtgg tttattattt ggctacgata
    32281 ccgccgttat ttccggtact gttgagtcac tcaataccgt ctttgttgct ccacaaaact
    32341 taagtgaatc cgctgccaac tccctgttag gattttgcgt agccagcgct ctgattggtt
    32401 gcatcatcgg cggtgccctc ggtggttatt gcagtaaccg cttcggccgt cgtgattcac
    32461 ttaagatttc tgctgtcctg ttttttattt ctggtgtagg ttctgcctgg ccagaacttg
    32521 gttttacctc tataaacccg gacaacacag tacctattta tctggcaggt tatgtcccgg
    32581 aatttgttat ttatcgtatt attggcggta ttggcgttgg tttagcctca atgctttcac
    32641 caatgtatat tgcggaactg gctccagctc atattcgcgg aaaactggtt tcatttaacc
    32701 agtttgcgat tattttcggg caacttttag tttactgcgt aaactatttt attgcccgtt
    32761 ccggtgatgc cagctggctg aatactgacg gcaggcgtta tatgtttgcc tcggaatgta
    32821 tccctgcact gctgttctta atgctgctgt ataccgtgcc agaaagtcct cgctggctga
    32881 tgtcacgcgg caagcaagaa cagacggaaa gtatcctgcg caaaattatg ggcaacacgc
    32941 ttgcaactca ggcagtacag gaaattaaac actccctgga tcatggccgc aaaaccggtg
    33001 gtcgtctgct gatgtttggc gtgggcgtga ttgtaatcgg cgttatgctc tccatcttcc
    33061 agcaatttgt cggcatcaat gtggtgctgt actacgcgcc ggaagtgttc aaaacgctgg
    33121 gggccagcac ggatatcgcg ctgttgcaga ccattattgt cggagttatc aacctcacct
    33181 ttaccgtact ggcaattatg actgtggata aatttggtcg taagccactg caaattatcg
    33241 gcgcactcgg aatggcaatc ggtatgttta gcctcggtac cgcgttttac actcaggcat
    33301 cgggtattgt ggcgctactg tcgatgctgt tctatgttgc cgcctttgcc atgtcctggg
    33361 gtccggtatg ctgggtactg ctgtcggaaa tcttcccgaa tgctattcgt ggtaaagcgc
    33421 tggcaatcgc ggtggcggcc cagtggctgg cgaactactt cgtctcctgg accttcccga
    33481 tgatggacaa aaactcctgg ctggtggccc atttccacaa cggtttctcc tactggattt
    33541 acggttgtat gggcgttctg gcagcactgt ttatgtggaa atttgtcccg gaaaccaaag
    33601 gtaaaaccct tgaggagctg gaagcgctct gggaaccgga aacgaagaaa acacaacaaa
    33661 ctgctacgct gtaatcttcc tgtccagcac gccgcgccat ttcggcgtgc tgtcttttta
    33721 ctcccgcttc agccgtttcg aattacacag ccacagggtg atcaccagta acaggatcgc
    33781 agccgagtag atcaacacat ccagtggcga tttatgatcg acgatgatca agcgcacaat
    33841 ggcggtgatc ccaatataga caaagtaacg tagcgggaag tgaaaaccgg actgaaagta
    33901 cttcacaatc agcgcgataa attcgaaata gagaaagtaa accaccagtc cttctaccag
    33961 ctcatatttg ctggtttgtt ctggcgcgaa cagcacatca gccagatgca ccgtttcttt
    34021 gccgaggaag acaaccaaaa tcaggccgag gcacagcagg ccaagattga gtacggtctg
    34081 caaaatggtg gagataaact ccacgcgcgg acgagagagt gacgtcatag cagtacctcc
    34141 ttcacagtgg cgagcttcct gtataacgca aaaatgtgac ggagatctat attttttgtg
    34201 tatgttttgt tcaacctgga tttggagcgc gcctccacca aagccggata tacctacccg
    34261 taggccggat aagccgcgcc tggcggcgca tccggcaaga caaaccgcac caaagccggt
    34321 aattcaaaaa tcacccattc aggtgattcc cagccaccag caatgcgcga aaaatcgaca
    34381 atgttcctct ggaacaaatg aaacgtaacc tgataaaggt ggtaaaaatg aagccttatt
    34441 ttgctgcttt gatgttatca gtctctgttc ttcctgctta tgctggtccg ctcggtactg
    34501 ctgataaagc cgacctaccg caaagcaatg tctccagccc aatgatggcc cagtcgctca
    34561 ggcaaccaga cctgcaaccg atatcgacgg acaggaagac agaatgtttt cgcctttaca
    34621 caccagatcg taaaccgggg gtgaactgcg ttcctgatgg ctcaacaggt cattaagaat
    34681 gagggtgcca gtgagtgcga aaactggcac ctttcccatg acttagcgga aatttacgct
    34741 acggtcgctg gtcatgtcat acaactggaa cttacgccca agttgctgac caccgtcacg
    34801 cgtcagcggc gtccagccaa tcgccgcacg gctgcgtgtc gggccagacg agaagagatc
    34861 caacggtacc gagacataca cgcctttggt gaagtcccct tcgccgtact cctctttcga
    34921 aacgttggtg atcgtggcat agccacccac caccacgccg ctgtcaaagc gtttagcgat
    34981 ctccagcgtg ccgcctttat cccctgccag atactgcccg acgctggctt tcaccaacac
    35041 gtcctgggcg aaagatggcg tccagtaggc ggtcagatgt ccggttttca cgctgtagtc
    35101 ggtgaatttc atcatatctt ttgcactacg ccagtcgcgc tgtttaacgt agttggcatc
    35161 cagaccaaac gcccagttac tgtccagcgg acgatacaac acttcggccc ccgcaccgcc
    35221 aaacatggtt tcgagataac ctccatagac ctgaccgtag aagccgttgc ccagatgctg
    35281 gaagtagttg gcttgcaggt tattcacata gacatcgttc tgcacatact cgcgcacatg
    35341 ggtacgaacg cgcggtaagt gcgagtcctg cggtggattg gtgtagttaa acttgtcgta
    35401 gttgttggca agatttgcaa acaggctgcc ggtggtcagt agatggtccg tcagccacaa
    35461 atctgccgtt cccatcacgc ccagctgata catgtaaaag ttttccgggc caccgaccga
    35521 ctggttcagc accggatcga tatggaaatc aaagcgtgat ttgtcgatat accagccctg
    35581 ctcggtggac ttcggaacca ctggctcgac gcgtttgtgc gccagcgtcg tttcgtggcc
    35641 caacggctct ccggcgagat gatttttcag gctggcgaca tcggtttccg tcgtcacttg
    35701 cggcatgtta aggcgatttt ccgtaatgcg gatcgttttg atcccatccg gcagatcgtt
    35761 catcacgatc cgattagcac ggatgatccc ttcgcgcgaa tcacgatatt tcacctgctc
    35821 gccggtaaca tacagcgtat cgcctttcgc ctggatctgc ggatcggcaa gtccggcgtt
    35881 gtatttcagc aacgttaact gattcgccac caccgaatgc tgcaaaatgg catcctgcgg
    35941 ctgcggttga tattgcgggc gggcgttatc gatgtaggac gggcgcagat cgttaaagtt
    36001 ggtgcgcagc gtgacgccaa acataaaggt gttgccacgt tcatagctaa ggttaacgtc
    36061 ggcccaatcg gtaacgcgat aaatcgcccc gacgttaaac ttactttttt gctccagctt
    36121 cccggcaaaa tcctgctgat aattattacc ttcatactcc agtttcagac gcagcggttg
    36181 ccagggcgtc tggtattcca cgccgccaaa cagtgaggca ggaccgtgga acatctggct
    36241 accgtcgata gatcccgcct gtttgtagct gttatcgcga tagcaatatt tatcactggc
    36301 tgaacagagc ggatttttta cattaccgct ggtgcccaga tatccccagc ccaggccgag
    36361 cgtaaaatcg aacggccccc aggctttgct ggcaacaaga tattccgcat caaacagccc
    36421 cgtaccgccg atatcccgcg caccaaccgc cacttgcggc agccagtaac tctcttccca
    36481 caaacgcagt ttgagatcga aggctttatc tttatacgtt tgatcgccag agaacgcttc
    36541 gacgctgctg tactgccggg tgcgcacgtc ggtgtagcgc agcgttgttt ccagccacgg
    36601 gaagagttgc actgaagctg agtaataacg gtactgatcg ttatcgcgat agttcagact
    36661 caactcccct tcccgtgcca tacgcgcggt gggagtttgt aataatccta cgccaccgaa
    36721 atccgactgc gacggaccaa tgggcgcagg atatgtttcg ccgtagcaag ctgtgctaat
    36781 gcccagcgcc agtaagctaa gcagatgtct ttttttcatt attggggtat ccgctgcgtc
    36841 agagtctgaa gaatgtcggc gttaagggca tcaggcgtct cactccagac ggaatctgcg
    36901 aggccaacat aaataatgct gccgggcatc ggctctacgt gacgcttgtt ccagtaagcc
    36961 accggcgctt tttgcgtgcg tccgtccggg taaacgaccc acgcgtagct gcgatccgca
    37021 ccgctgagca ggctctggtc agagagatag ctcgccacgt cgcgacctgg agtgaatggc
    37081 tgattgccag gatggctgat aagcccgaac aacgtgaccg tggacggcgg tggtccgacc
    37141 cacagcgtgt agttgccctg caacggcggg ttaccgcgtt cggcaacgcg gacgatatcg
    37201 ggatcaagat tgattttttg cctgcctgtc actttcaatg cctggatttg ctggcgtaag
    37261 gcgttaatgg cggcggcatc gtcggtgctg gaatctgcgc cctgttctgc cagccgtgtc
    37321 agcaacgcct gctgctgacg caatgctgcc gccgttgcca gctcttcgct aatcaccgca
    37381 ccaggccacc agctgtttgc cagtcgaggt tgccctacca gatcgattaa atgctctgcg
    37441 cccgttagcg ttttggcctc actgctgcca ttgctgaaca ccttgacggt tcctgccgcg
    37501 aagaccgatg acgctcccac actcaaaagc aacgcgacaa tagtttgttt aatcattgtt
    37561 ttgccgcctt gatgagagtg gttttcaccg ggaaataatc cgcgccgaga tactgttccg
    37621 actggcgaat ttgcccttcg ctgtcgatcc agtagcggtt atgccagcgc gcctggtcgg
    37681 tggagacttc ttcgtccagc acgcgaacca gggtttcatc gctgccgacc ttgacggtat
    37741 cggtgccatc ccatttgaag actgagcgtg cggtggcgta gcgtacctgc tggtactcgg
    37801 tccagcccat cgtgcgcgtc catgttgcac catcaacaat ttgtgcgggt ttaatcagcg
    37861 ggtcggcggc aaggttattc acttcaatca ggttgtcgcc gccaagcaaa gtcttcacca
    37921 gacgaccatg ttgtgtgaca agagtggctt gatcctgagt gacccatttt tgttgtccgt
    37981 cttcagcgaa ggccagcacc acaaacaatt gcggcccgcc attaagctgc atgtactggc
    38041 tggcgtaggg catattttga atatcatcgt ccgtcagctg tacgcctggc gtgccgaaca
    38101 gactgtccca cagtgaattg cccagctctt tagtcgtggc tgaacaggcc tgtaatagca
    38161 ggcagataag aatgagtgca ggtcgcttca cgactcttct ccagaggggc agaataacca
    38221 caccggagtg tggttatggt aaatcccttc atacttcaag ccacagatgc aatgactcaa
    38281 attattttgg ggatacatac tttattactg ggtactggtg gtcgtggttg tggtggtccc
    38341 ggtattagaa ccatcaccgc caccggttgc cgcgagcgcg acacccaccg ccgagcttac
    38401 ggtgctgacg ctggtcgcgg aggaactccc cgccgaaacc gacgttgctg ccgactctgc
    38461 cgcttcgccc acctgtacag gtgcagccca cgcagaagtc gccgcaagcg cagatatggc
    38521 aaaaatgcca tacagaactt ttttcataac aatttccctt cattgaatga atggagattt
    38581 acccaaaaac atttcgggcg ggatcgagta tacacaacta aagcatgcga attacggcgc
    38641 ggggaattag cgatggagtt ttaggataat tggaatgaat atcaaatata agtttcaact
    38701 tatatttaaa taacaataaa aatcataagc ttaaaataaa aaacaaaagt aattaatctg
    38761 cgtattctcc gaaataaaca acgattaaaa atctatttta ggattaacct cacggtatga
    38821 tttccgttaa atcacagaca aggggattta tctgataaaa caatgccgac ttattgtcgg
    38881 cattgttttt aaggttattt aactcctcat actatgcatt aatgtcgata ttggcatcag
    38941 gctccggttg ccggatgcgg cgtaaacgcc ttatccggcc tacatatcgg catcaggcac
    39001 gcatgatgat taaccgcgcc acgctttata gcggttaatc agaccattgg tcgagctatc
    39061 gtggctgctg atttctttat catctttcag ctctggcaga atacggtttg ccagttgttt
    39121 acccagctcc acgccccact ggtcgaaggt gaagatgttc aggatcacgc cctgagtaaa
    39181 gattttgtgc tcatacagcg caatcaacgc acccaggctg aacggagtga tttcgcgcag
    39241 caggatggag ttggtcgggc ggttaccttc gaacactttg aacggcacca cgtagtcaag
    39301 cgttgccgga tctttaccct gatcgcgata ttcctgctca accacttcgc gggatttacc
    39361 aaacgccagc gcttcggtct gggcgaagaa gttagacagc agtttctggt ggtgatcaga
    39421 gagcgggtta tgggtgatag ccggggcgat gaaatcgcac ggtaccattt tggttccctg
    39481 gtggatcagc tggtagaacg cgtgctgacc gttagtgcct ggttcacccc agataatcgg
    39541 gccagtctgg taatccacaa cgttaccgtt acggtcaaca tacttaccgt tggactccat
    39601 attgccctgc tggaagtacg ccgcgaaacg gtgcatatac tggtcatacg gcagaatcgc
    39661 ttcagtttcc gcaccaaaga aattgttgta ccagatgcca atcagcgcca gcaggacagg
    39721 caggtttttc tcggcaggcg tggtggagaa atgcttgtcc attgcgtgtg cgccagaaag
    39781 cagttcaacg aagttatcaa agccgatgga gagaacaatc gacaggccaa tcgctgacca
    39841 caaagagtaa cggccgccga cccagtccca gaactcgaac atgttggcag tatcaatacc
    39901 aaactcgcca acggctttgg cattggttga gagcgccgca aaatgtttcg caacgtgttt
    39961 ttcatcacct gccgctttca ggaaccagtc acgcgcgcta tgggcgttgg tcatggtttc
    40021 ctgagtggtg aaggttttag atgctaccag gaacagcgtg gtttccgggt ttactttttt
    40081 cagcacttcc gagatgtgag tcccatcgac gttagaaaca aagtgcatgt tcaggtggtt
    40141 tttgtacgga cgcagtgctt cggtcaccat gtacgggccg aggtcagaac cgccgatccc
    40201 gatgttcact acgtcagtga ttgctttgcc ggtataaccc ttccactcac cggagataat
    40261 cgcttccgag aaggttttca tcttctccag caccgcgttg acttctggca taacatcttt
    40321 gccatcaacc agaatcgggg tattgctacg attacgcagc gctacgtgca gcacggcgcg
    40381 gttttcagtg cggttgatct tctcgccaga gaacatcgac ttaatcgcgc ccgccagatc
    40441 gcactctttc gccagatcct gtaatttcgc cagcgtctct tcagtgatgc ggtttttgga
    40501 gtaatccacc agcatctgat cgtcgaaggt tgcggagaac ttagaaaaac gatcgccgtc
    40561 tttagcaaaa agatcggcga tcgtaacgtc tttcatttca tcgaagtgtt tctgtagtgc
    40621 ctgccaggca gcggtctgcg ttggattgat gtttttcatt agcaatactc ttctgatttt
    40681 gagaattgtg actttggaag attgtagcgc cagtcacaga aaaatgtgat ggttttagtg
    40741 ccgttagtgt aatgttgagt gtaaaccctt agcgcagtga agcatttatt agctgaacta
    40801 ctgaccgcca ggagtggatg aaaaatccgc atgaccccgt cgttgacaac cgccccgctc
    40861 accctttatt tataaatgta ctacctgcgc tagcgcaggc cagaagaggc gcgttgccca
    40921 agtaacggtg ttggaggagc cagtcctgtg ataacacctg agggggtgca tcgccgaggt
    40981 gattgaacgg ctggccacgt tcatcatcgg ctacaggggc tgaatcccct gggttgtcac
    41041 cagaagcgtt cgcagtcggg cgtttcgcaa gtggtggagc acttctgggt gaaaatagta
    41101 gcgaagtatc gctctgcgcc cacccgtctt ccgctcttcc cttgtgccaa ggctgaaaat
    41161 ggatcccctg acacgaggta gttatgtctg aaattgttgt ctccaaattt ggcggtacca
    41221 gcgtagctga ttttgacgcc atgaaccgca gcgctgatat tgtgctttct gatgccaacg
    41281 tgcgtttagt agtcctctcg gcttctgctg gtatcactaa tctgctggtc gctttagcgg
    41341 aagggctgga acctggcgag cgattcgaaa aactcgacgc tattcgcaac atccagtttg
    41401 ccattctgga acgtctgcgt tacccgaacg ttatccgtga agagattgaa cgtctgctgg
    41461 agaacattac tgttctggca gaagcggcgg cgctggcaac gtctccggcg ctgacagatg
    41521 agctggtcag ccacggcgag ctgatgtcga ccctgctgtt tgtcgaaatc ctgcgcgaac
    41581 gcgatgttca ggcacagtgg tttgatgtac gtaaagtgat gcgtaccaac gaccgatttg
    41641 gtcgtgcaga gccagatgta gccgcgctgg cggaactggc cgcgctgcag ctgctcccac
    41701 gcctcaatga aggcttagtg atcacccagg gatttatcgg tagcgaaaat aaaggtcgta
    41761 caacgacgct tggccgtgga ggcagcgatt atacggcagc cttgctggcg gaggctttac
    41821 acgcatctcg tgttgatatc tggaccgacg tcccgggcat ctacaccacc gatccacgcg
    41881 tagtttccgc agcaaaacgc attgatgaaa tcgcgtttgc cgaagcggca gagatggcaa
    41941 cttttggtgc aaaagtactg catccggcaa cgttgctacc cgcagtacgc agcgatatcc
    42001 cggtctttgt cggctccagc aaagacccac gcgcaggtgg tacgctggtg tgcaataaaa
    42061 ctgaaaatcc gccgctgttc cgcgcgctgg cgcttcgtcg caatcagact ctgctcactt
    42121 tgcacagcct gaatatgctg cattctcgcg gcttcctcgc ggaagttttc ggcatcctcg
    42181 cgcggcataa tatttcggta gacttaatca ccacgtcaga agtgagcgtg gcattaaccc
    42241 ttgataccac cggttcaacc tccactggcg atacgttgct gacgcaatct ctgctgatgg
    42301 agctttccgc actgtgccgg gtggaggtgg aagaaggtct ggcgctggtc gcgttgattg
    42361 gcaatgacct gtcaaaagcc tgcggcgttg gcaaag
//
LOCUS       DAJTRD010000027        40535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTRD010000027 DAJTRD010000000
VERSION     DAJTRD010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.027"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..957)
                     /locus_tag="OO315_001593"
     CDS             complement(<1..957)
                     /locus_tag="OO315_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HCR5890548.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKIEVNLGAGEAGS"
     gene            1203..1829
                     /gene="gstB"
                     /locus_tag="OO315_001594"
     CDS             1203..1829
                     /gene="gstB"
                     /locus_tag="OO315_001594"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5890549.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1826..2940)
                     /gene="yliI"
                     /locus_tag="OO315_001595"
                     /pseudo
     CDS             complement(1826..2940)
                     /gene="yliI"
                     /locus_tag="OO315_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(3051..3434)
                     /gene="bssR"
                     /locus_tag="OO315_001596"
     CDS             complement(3051..3434)
                     /gene="bssR"
                     /locus_tag="OO315_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5890550.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            3647..4972
                     /gene="rimO"
                     /locus_tag="OO315_001597"
     CDS             3647..4972
                     /gene="rimO"
                     /locus_tag="OO315_001597"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5890551.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(5019..6347)
                     /locus_tag="OO315_001598"
     CDS             complement(5019..6347)
                     /locus_tag="OO315_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5890552.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(6355..8697)
                     /locus_tag="OO315_001599"
                     /pseudo
     CDS             complement(6355..8697)
                     /locus_tag="OO315_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8875..9786)
                     /gene="gsiD"
                     /locus_tag="OO315_001600"
     CDS             complement(8875..9786)
                     /gene="gsiD"
                     /locus_tag="OO315_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5890553.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9789..10709)
                     /gene="gsiC"
                     /locus_tag="OO315_001601"
     CDS             complement(9789..10709)
                     /gene="gsiC"
                     /locus_tag="OO315_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5890554.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10727..12265)
                     /gene="gsiB"
                     /locus_tag="OO315_001602"
     CDS             complement(10727..12265)
                     /gene="gsiB"
                     /locus_tag="OO315_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5890555.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(12285..14156)
                     /gene="gsiA"
                     /locus_tag="OO315_001603"
     CDS             complement(12285..14156)
                     /gene="gsiA"
                     /locus_tag="OO315_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5890556.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(14143..15108)
                     /gene="iaaA"
                     /locus_tag="OO315_001604"
     CDS             complement(14143..15108)
                     /gene="iaaA"
                     /locus_tag="OO315_001604"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5890557.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            15312..16547
                     /gene="moeA"
                     /locus_tag="OO315_001605"
     CDS             15312..16547
                     /gene="moeA"
                     /locus_tag="OO315_001605"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5890558.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            16547..17296
                     /gene="moeB"
                     /locus_tag="OO315_001606"
     CDS             16547..17296
                     /gene="moeB"
                     /locus_tag="OO315_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5890559.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(17475..18137)
                     /gene="fsa"
                     /locus_tag="OO315_001607"
     CDS             complement(17475..18137)
                     /gene="fsa"
                     /locus_tag="OO315_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5890560.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            18268..19167
                     /locus_tag="OO315_001608"
     CDS             18268..19167
                     /locus_tag="OO315_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5890561.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            19173..21605
                     /locus_tag="OO315_001609"
     CDS             19173..21605
                     /locus_tag="OO315_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5890562.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21751..22566
                     /gene="ybiV"
                     /locus_tag="OO315_001610"
     CDS             21751..22566
                     /gene="ybiV"
                     /locus_tag="OO315_001610"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5890563.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22718..23983
                     /locus_tag="OO315_001611"
     CDS             22718..23983
                     /locus_tag="OO315_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5890564.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(24224..25816)
                     /locus_tag="OO315_001612"
     CDS             complement(24224..25816)
                     /locus_tag="OO315_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5890565.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            26035..26955
                     /gene="ldtB"
                     /locus_tag="OO315_001613"
     CDS             26035..26955
                     /gene="ldtB"
                     /locus_tag="OO315_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5890566.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(27013..28131)
                     /locus_tag="OO315_001614"
     CDS             complement(27013..28131)
                     /locus_tag="OO315_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5890567.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(28128..28595)
                     /gene="mntR"
                     /locus_tag="OO315_001615"
     CDS             complement(28128..28595)
                     /gene="mntR"
                     /locus_tag="OO315_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5890568.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28781..28909
                     /gene="mntS"
                     /locus_tag="OO315_001616"
     CDS             28781..28909
                     /gene="mntS"
                     /locus_tag="OO315_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5890569.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            29181..30764
                     /gene="opgE"
                     /locus_tag="OO315_001617"
     CDS             29181..30764
                     /gene="opgE"
                     /locus_tag="OO315_001617"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5890570.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30812..31327)
                     /gene="ompX"
                     /locus_tag="OO315_001618"
     CDS             complement(30812..31327)
                     /gene="ompX"
                     /locus_tag="OO315_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5890571.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(31380..31445)
                     /locus_tag="OO315_001619"
     CDS             complement(31380..31445)
                     /locus_tag="OO315_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890572.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31680..32567
                     /gene="rhtA"
                     /locus_tag="OO315_001620"
     CDS             31680..32567
                     /gene="rhtA"
                     /locus_tag="OO315_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5890573.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32866..33369
                     /gene="dps"
                     /locus_tag="OO315_001621"
                     /gene_synonym="pexB"
     CDS             32866..33369
                     /gene="dps"
                     /locus_tag="OO315_001621"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5890574.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33773..34519
                     /gene="glnH"
                     /locus_tag="OO315_001622"
     CDS             33773..34519
                     /gene="glnH"
                     /locus_tag="OO315_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5890575.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            34658..35317
                     /gene="glnP"
                     /locus_tag="OO315_001623"
     CDS             34658..35317
                     /gene="glnP"
                     /locus_tag="OO315_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5890576.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            35314..36036
                     /gene="glnQ"
                     /locus_tag="OO315_001624"
     CDS             35314..36036
                     /gene="glnQ"
                     /locus_tag="OO315_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5890577.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            36297..38521
                     /gene="ybiO"
                     /locus_tag="OO315_001625"
                     /pseudo
     CDS             36297..38521
                     /gene="ybiO"
                     /locus_tag="OO315_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(38518..39444)
                     /gene="rlmF"
                     /locus_tag="OO315_001626"
     CDS             complement(38518..39444)
                     /gene="rlmF"
                     /locus_tag="OO315_001626"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5890578.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39720..39980
                     /locus_tag="OO315_001627"
     CDS             39720..39980
                     /locus_tag="OO315_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5890579.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            40245..>40535
                     /locus_tag="OO315_001628"
     CDS             40245..>40535
                     /locus_tag="OO315_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890580.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKN"
BASE COUNT        10096 a        10714 c         9864 g         9861 t
ORIGIN      
        1 ggagcccgct tcgcctgccc cgagattcac ttcgatctta tcaccaaacc agacgcgctg
       61 agtcacaaag gtggcatcag gtttaattgg cgtcacagtt tcaaagaagc ggaaacccca
      121 ggtcaataat ttctcagact cattaaaacg gatacggtca gttttcgccc ccagcactac
      181 ggagattaaa cgcatatcac cctgggtagc cgaagcaacc agattatatc ccgcacctgc
      241 cgtggttcct gtcttcatgc catcaacatt cagattgctg ctccacagca gacggttacg
      301 gttaggctga cgaattttgt tgaaggtgaa ctctttctct ttatgaatgg cgtactcttc
      361 cggcacatcg tggatcaatg ctttacccag caatgccata tcgcgcgcgg tactgaactg
      421 ccccggcgca tccaggccgt gcaccgtctg gaaggtagtg ttggtcagcc ccagtttttt
      481 ggcataacca ttcatcagac caataaagga ctcctggctc ccggcaacgt aatctgccag
      541 cgcaatacag gcgtcattac cggactggat aatcacacct ttgttcaagt ctgccaccga
      601 aacctgatcg cccggtttga ggaacattac cgatgaacca cgcagtgccg gatttcccgt
      661 cgcccaggca tctttaccga ccgtcaccat atcggtgagt ttaatcttat cggccttaag
      721 cgcctgccca accacatagc tggtcatgat tttagtcagg ctcgcaggat ccagtttctc
      781 atccgcattg ccttctgcca gcactttacc gctggcgtaa tccattaaaa tccatgcacg
      841 cgcatccacg ctcggcgctt caacggtttg ttcggccgcg aatgccgttg gggcaaaaag
      901 gaataaaaat gcagaacccg ctgcaagacc acgaaggaga gaggagtatt gcgtcataaa
      961 tgccacccga gtatccattc aaaaaaatac gttatatcgc cgtatgataa gttcggtgat
     1021 taataacgca cacacaaact taaagaaacc gtaagttggt aaagttttta aagtttatgc
     1081 aatatcctgc cgcttcgctg cttctggtgt catttttttg ccagctgttg cttaaaagtt
     1141 aactttactt caccatcgga gtcctgttca gtctgtggct gcctgtcgga aaaggggtga
     1201 gtatgattac gctgtggggt cggaataatt caactaacgt aaaaaaagta ttgctgacgc
     1261 tcgaagaact ggaactacct tatgagcaaa ttctcgcggg ccgtgagttt gggataaatc
     1321 acgatgctga ttttctggcg atgaacccta acgggctggt gccgttgttg cgtgacgacg
     1381 aaagtgatct cattctttgg gaatcaaacg ccattgtccg ctatctggcg gcacagtacg
     1441 ggcaaaaacg cctgtggatc gactcaccgg cacgtcgtgc ggaagcggaa aaatggatgg
     1501 actgggcaaa ccagacgctc agtaatgctc atcgcgggat cctgatggga ttagtcagaa
     1561 caccaccgga agagcgcgat caggccgcca ttgatgccag ttgcaaagag tgcgacgccc
     1621 tgtttgccct gctcgatgcg gaactggcaa aagtaaaatg gttctctggc gacgagtttg
     1681 gtgtgggcga tatcgctatc gcaccgttca tctacaattt gtttaacgtt ggcctgacct
     1741 ggacaccgcg tccaaatctg caacgctggt atcagcaact cactgaacgc cccgcagtgc
     1801 gcaaagtggt gatgattccc gttagctaat tgcgtgggct aactttaagt aattccccac
     1861 tggactcgtc ggtgagaacg tataaataac cgtcgggtcc agtgcgaaca tcacgaattc
     1921 gctgccctct gtccgttaaa atacggccat cttctgtcac tttgtcgccg ttgacgctca
     1981 tcacaatgac atctttatct ttcagctccc aataaataat ttttgctgcc actgggggaa
     2041 tttatcgctg ttatagaagg ccatgccgct cacagcgggc gaatctttcc agtaaaaaac
     2101 aggttgctcg gtcccggcga cgatctcccc tttcgcttcc ggtatcttaa agcctgaata
     2161 gttgattccc caggttgcca gcggccagcc gtagtttttg cctttttgcg ggatattaat
     2221 ttcatcacca ccgcgcgggc catgttcatt cagccacagt gcattactcc acggattcat
     2281 cgccattcct tgcggattac gaatgccata agaccagatc tcggcgcgcg caccggattc
     2341 ctttataaaa ggattatcat ccgggatttc gccctggtcg gtcagacgca ccagtttgcc
     2401 ctgtaattta tccagatcct gcgccgtcgg gcgctgattg ttttcgccca gagcaataaa
     2461 aagataacct ttaccgtcga ataccagccg cccgccaaaa tggttgccgg tagacagttt
     2521 tggcatctgg cggaagacgg tgcggaagtc ggtcactttt gagagatcat cacttaagcg
     2581 gccataaccc acagcagttc cggctttgcc atcatcgcca acttcggaat aacttaacca
     2641 gatgcggcga gactgagcaa aatcaggcgc taaaaccacg tccagcaggc cgccctgccc
     2701 gtgcgcccaa acgtccggaa ctccggaaag cggcgcagat aatccttttc ctgcttgcca
     2761 gtgacgcaac tcgccgccgc gcagagtgat taacataccg tgattatcgg gtaaaaaggc
     2821 cagtgcccag ggatggtcga gtttgtcttg cagtacttcg acatttaccg ttgcaggagc
     2881 cgcccagaga gcggaagaaa gacaaataag gggcacaagg aaaaaggatt gtcgatgcat
     2941 aacgcctccg gattgactct ggcttaaagc gtagtcagtg gaggagataa caaattcatt
     3001 tttacaaaaa cttaaacatg aagggggaga cgctttctcc cccttagttt tcaggccttc
     3061 tcaagcatgg cgtgcttctg caggctctgg atactcagcg ttaagctcat cagacaattt
     3121 tcaagcttat cggcgttgac ggtaataaca gtcgggcaat catggtgccc actcatcaaa
     3181 catactgcgg ctgtcgctaa tgcttcttca gcatgatgaa gagcactcca ctcttcctga
     3241 tccagatgaa gattcaaccg cagcgattta tcgtgcagtt cgcgattcag tttaaaaaag
     3301 ttatctcgta gatgattgct ttcgctgacg gacatgtatc cttttgcctt cctcagttgc
     3361 acgtatgcag cgaggtcaac gcgtgcgtcg atcaaccggt tcagcagatc gattcgctgt
     3421 ctgtcaacga acatacttcg atcctcctct ttccagcctt gttgcaatta aatagtatgg
     3481 ctattttttg agcaaaatgc tgtccaggat caattcttca cgccgaatta acaaaattta
     3541 catttccttc gcctccgcca gcgaatgaga ttgcgataca aattttatcc agaactctgt
     3601 agaatccctg ccctgacctt tcctgtaact gaatattttt taagctatga gcaaagtaac
     3661 tccccagccg aaaatcggct ttgtttccct tggctgtccg aaaaaccttg tcgattcaga
     3721 gcgtattctc accgaactcc gcactgaagg ttatgacgtg gtaccgagct atgacgatgc
     3781 gaacatggtg atcgtcaaca cctgcggctt tattgacagc gcggtacaag aatcactgga
     3841 agccattggt gaagcgttga atgaaaacgg caaggtaatt gtgaccggtt gtctgggggc
     3901 aaaagaagat cagatccgcg aagtccaccc gaaagtgctg gaaatcaccg ggcctcatag
     3961 ctatgagcag gttctggagc acgttcatca ctacgtgcca aaaccgaaac acaacccatt
     4021 cctgagcctg gtgccagaac aaggtgtgaa actgacgccg cgtcattatg cctatctgaa
     4081 aatttcggaa ggctgtaatc accgctgcac cttctgcatt attccgtcta tgcgcggcga
     4141 cctgatgagc cgtccgattg gcgaagtgtt aagtgaagcg aaacgtctgg tagatgcggg
     4201 cgttaaagag attctggtga tctcgcagga tacttccgcc tatggcgttg atgttaaaca
     4261 tcgtactggc ttccacaacg gcgagccggt aaaaaccagc atggtcagcc tgtgcgaaca
     4321 gttatcgaaa ctggggatct ggacacgtct gcactacgtt tacccttatc cgcatgtgga
     4381 cgacgtcatc ccactgatgg cagaaggcaa aatcctgccg tatctggaca ttccgttgca
     4441 gcacgccagc ccgcgcattc tcaaactgat gaagcgtccg ggttctgtag atcgccaact
     4501 ggcgcgcatc aaacagtggc gcgaaatctg cccggaactg accctacgct caacctttat
     4561 tgtcggcttc cctggcgaga cggaagaaga tttccagatg ctactcgact tcctgaaaga
     4621 agcacgtctg gatcgcgttg gctgctttaa atacagcccg gttgaaggtg cagacgccaa
     4681 tgccctgcct gaccaggttc cggaagaagt gaaagaagaa cgctggaacc gtttcatgca
     4741 gctgcagcag cagatttccg ccgagcgcct gcaagagaaa gtgggccgtg aaattctggt
     4801 gattatcgac gaagtggacg aagaaggcgc gattggtcgc agcatggcag atgcaccgga
     4861 aatcgacggc gcggtttatc tcaatggtga aaccaacgtt aagccgggtg atatcctgcg
     4921 tgtgaaagtc gagcacgccg atgagtacga tttgtggggt agccgggttt aattaattca
     4981 ggccgggcaa aatcatttgt ccggcctgcg aataatgatt actgccagct aatctgtgcc
     5041 ctgccgtttt tcttactctt atacagccgc tcatccgcca gcattttcaa gcgttcgacg
     5101 gtagtacatt catttgaatg cgctacaccg atactggtgc ttaaattaat gcttcccacc
     5161 tggaaatgaa cgatttcttt tagtatcgat agcaactctt ccagcgtgcc gccagtataa
     5221 atcaccgcaa attcgtcacc tccgaagcgg tagactttac ctttatctac cgggatctgt
     5281 gatttaagga ttttcaccac tttcatcaac acttcatcgc ccagcaaatg accgaaggtg
     5341 tcgttaatgt ttttgaattt atcgacgtca aacagacaca aataaccgcc tttttccggc
     5401 agcgctttta aatcttcatc aaaggatttt ctgcccattg ctttggtcag gtcgtccagg
     5461 aacgccgagt tatacatact gtagagtttt ttgacatcgg cagccgaaaa gaaaagcccg
     5521 ccatccagta cgcgttcaaa atggttatta atgatggctt ctttctccac aataccacga
     5581 ttaatcactc tgcgtaccag atagaggcaa ctggcaccca atatcactgc caccaatgcg
     5641 gcaaagaaaa taatcaatgt acaaacccgc aaataaacat cttttttata cgcaaatacg
     5701 tacaatccgg tcggcaaata ggcgaagtgc tccatcgctt ccgaaacggt gaagatggcg
     5761 tttttagcat tatattcatc accggtcacc ggtttaccgt tcttaaatag agagaacatc
     5821 aggttctcgc tgtccatcac cagcgatttt gacagtaaag aaaaacgctc cagaccaagc
     5881 aaaaatatca gactacgatt gtgagttacc ggatagaggt aatagacgaa ggttttaccg
     5941 tcaaaagaga taaaccctgt atttttgaca tgtcgatact tcacaacctg cataatatct
     6001 gcacaataat ttttctcgaa ctcctttacc tgaatacatt cgtcactgga aatcagataa
     6061 ggaccgacat cgtacaaata agagggggaa accgagtcgg cgtaagctgt gcgcaaacca
     6121 accaacagct gttcctccgt gggatttagc gtgttgataa gatcaattgc cacgctacgc
     6181 cgatattcag caatatttgc caccgcaaaa gcagtatcaa taacggactg tttcaggcta
     6241 tacacccgtt cctttaccat ttgagtgtag atcccgcagg caactgctga aatcatgata
     6301 aaaatcagca gactgactgt aagtacgaac ttattgattc tggacatacg taaactactc
     6361 ttttactaat tttccacttt tatcccaagc ggagaatggc atgggtttct ggtacagata
     6421 cccctgagcc tgaacacatc ctaatttaat taattcgcgc gcaacgtctt cgctctctac
     6481 gccttccgca atcaccggga tccggttata gcgtgccaga tcgaaaataa tgcgcacaat
     6541 ttttcccgca gtaccgaaaa tatcgccggt aaattgcgag tccaccttta tgctgtctgg
     6601 attaatttca taaaaacgtt tcaggtttga caaaccggtg ccaaagtcat caatcgatat
     6661 ccgtacccct tgtttacgca gttgttcaat attgagataa aaatgttcat taattcgctc
     6721 gatcacggtt tcagtgatct caacacagag gctgttcgcc ttcaggccat tttctttcat
     6781 gatggtggta aaacgctcat gaaagtgcgg ttcgtttaac tgccagggcg aaacattaat
     6841 atgcagtaag aaatcatcct tcagcgacgc acgttgacta aaatgtctga attaacccat
     6901 tgccgtttta ataatctgat aacccagctc attgataaac ccgatatctt cagcaatggt
     6961 aatgaacttc agcggtgaaa tgatcccccg ctccgcagaa acccaacggc acaacgcctc
     7021 cgcgctcaga atggcgcggg tgttaatatc aacgatcggt tgatagacga ggaatagttc
     7081 gccaatttcc attgcatggc gaatattacg cgcaatccag atattctctt ctatttcgtt
     7141 gagtttcgcg tcatcacaga aaataaactc ggagaacata tggtcacgga tactggaaat
     7201 cgccagtttc agtttttctg cgtaactcca ggcgttttcc ccctgcaaag gttcacaaat
     7261 ggcagactta tggatgcgca gattgcggta gccaaagctg ctgtcaaaat cgtttaccat
     7321 cgctatttta cgggtaaatg tctgtacatt ttcccgacac agaagtaaat agagatcggc
     7381 attgaatgtc acgatatcgc agcattgcgc atatttctcg gcaattcgct gacacaattt
     7441 cctgacgatt ttattgcgtt catcaacacc aaacaccgcc tccacggctt ccagaccaca
     7501 cagtttgatg atccccacag ccagatactg attattgttg gactcaataa tcgccatatt
     7561 tttaataaaa ccgctttcct gatcgtaaga gatcagtgag agcaatttat ttgaccgttc
     7621 aatttcggca tctttcattt ccgcgatcga atttttgatg ctgataatct ctttcgcatc
     7681 gccttcgcga attttcagag cctcgatctg atccgttgag tcgggcaaaa acgccagcgt
     7741 attaaccagc tcggtcattt cagaaaattg ccgacggaac acacgagtgg tcatgaaata
     7801 taccaccagc aacacgataa taatgaacgc aaacggtaga taaaaaagtt tatctgatgc
     7861 gttcaataaa ttaccgccag gataataaaa atagatttcg aacaatgcat tatttggcac
     7921 tgctgatgtc tttgcaatat actccgtatc gacagaatca ataatattat aattaccttc
     7981 gctattataa ataatcagtt tttcacgagc gagttgattc ttggcaatga tctcatgacc
     8041 ttgtttcaaa aatagaactt catcattagc gctgatttga tcaagatact cgttcagata
     8101 ccgccagttt aaatcgacat gaattttatc taccgctaac aaatcgtgat ctttgccgtt
     8161 gatacgtagc gtaaaagaga tacatttttt acccggacga tcaaagcaat tgtatgccgg
     8221 attccagctg ttgttagagt catctgaagc caccgaccac ggtcgtttcg atgggttaaa
     8281 atcggcaaaa ataacccgcc ctgactcgtc gacaagcggt ccatttactt gttcctgatg
     8341 ataaacaacg attttatcat tcgccctccg cgaaaagaga taatatttat tatcatcaag
     8401 taccagtccc accgagtcta aaaatggcat catttccatt ttcagaatca cggcgcgttg
     8461 aatctcctct gtatttttta ataaatacag tgagtccttt aatgtcaggg ccgaacgttc
     8521 aatatcttta aagaaatagc caacgttttt ctgaataagt tcaaagcggc tatcggcacg
     8581 ttttaccgcg cgttccgata ccagttgata gttaatcatg aaaaaaagac caataaatga
     8641 cagtgctgcc aataaaaata aaattatcag ccttatcttt tcgtataaac tcagcatgtt
     8701 aatacattta acaaaataac tatctgaacg ctgtgccgct gcaaaaatag tcccttgctg
     8761 cggcttttta accaaattac ccgattctac aaaatcagca gaaagttaca acacttgctt
     8821 agggcaataa tttcctgtga ggtgattacc ctttcaagca atattcaaac gtaactatcc
     8881 tttaattttc ggatccagcg catcgcgtaa accatcgccc aacaaattga acgccagtac
     8941 ggtcagaaaa atagccaggg ccggaaaaac agcgacatgc ggcgcgataa ccatatccgc
     9001 tcgtgcctca ttgagcattg ctccccactc tggtgtcggc ggctgcgcac cgaggccgag
     9061 aaatgacagg ctggcggcag agataatcga ggtgccaatg cgcatggtga aaaacaccac
     9121 gatagaagag acggtcccag gcaggatatg acgcaataaa atggtcatat cgctggcacc
     9181 aatactgcgt gctgactcaa taaaggtttg ctgtttcaac accagcgtgt tgccgcgcac
     9241 caggcgggca aacgcgggga tggaaaaaat ggcgactgcg ataatcacgt tagcaatgcc
     9301 gcttcccaac accgcaacaa cagcgatcgc cagtaaaata cccgggaagg caaacagcac
     9361 atcgcaaatg cgcatgatca gccgatccca ccagccttca taatatccag ccaacaagcc
     9421 cagcaacgtc cctatcgcca caccgataaa cacggcaaac acgcccgccg ccagcgagat
     9481 ttgcgcacca accaggacac ggctgaaaat gtcgcgcccc agtgaatcga cgccaaacca
     9541 gtgctgcaaa gaaggtccgt tattcagatt gtcataatca aaataatttt cggcgtcata
     9601 gggagcgatc cagcgtgcaa aaatggccac cacaatcaat aaaataacga ataatgcggc
     9661 ggtcatcgcc atatgctgac ggcgaaatcg tcgccagaat tcatgccacg gtgtacgtac
     9721 ctggtcaggt ttgaccagtg gcatggcgtt taacaccgcc tgacgtcgcc agttaaatag
     9781 tcgcatcctt acttgtacct gatagccggg ttaatggcgg cgtaaagcac atccaccact
     9841 aagttgataa gaataaattc cagcgagaaa agcagaattt ccgcctgaat caccgggtaa
     9901 tcgcgcattt ctacagagtc aacgagtaag cgtccaagtc cgggccagtt gaaaactttc
     9961 tccaccacga tagaaccacc gagcaaaaag ccaaactgta agcccatcat ggtcactacc
    10021 gggatcatcg cgttgcgtag cccgtgtttg aggacaaccc aggtttcgct cacccctttc
    10081 gccctcgcgg tacgcatata atcttcgctt aaaacgtcga caaatgacgc acgggtaaag
    10141 cgcgccatca cggcggccac tgccgcgccg agcgtcaggg agggtaaaat gtagtgctgc
    10201 caactgtctg ctcccacagt aggcagccag cccagttcaa cggagaatac ctgaattaaa
    10261 aacatcccca gcgcaaatgc cggaaacgag atccccgaca ccgcaatggt catactcaat
    10321 cgatccggcc aacgattacg ccagacggcg gcgataattc ccgccgccat accaaatatc
    10381 accgcccaga ccatactggt tatggtcagc catagcgttg gcataaagcg gctggcaatc
    10441 tcatcggcaa ccggacgacg cgacaccatc gacaggccaa aatccccctg cacagcattg
    10501 ctgatatagt gccagaactg gtgatacagc ggctgatcca accccagctg ctgacgcacc
    10561 agttctataa cctgcgcatc agcttcgggc ccggcaatca atcgcgccgg atcgccgggc
    10621 agcatgtgga caaataaaaa caccagcacc gagacgataa acagcgtcgg aatcaacccc
    10681 agtaatcgtt tgataacgta ataaagcatt ccactccctg cgttgcttat tgcaaatccg
    10741 cgtcttcaaa gctgaagccg gtatctggca tgatccaaaa accggtcagg tttttactgt
    10801 gtgccgacac cagtttttct accaccagcg ggatccacgg cgattcttgc cagatgatat
    10861 cctgcgccgc cttatataag cgggtctttt ccgccggatc attcgttttc agtgcctgag
    10921 ccaggaagtc atccacctgt ttattgctgt aaaacgcggt attaaacagc gtcggtggcc
    10981 agttttgcga ggcaaacagc ggcgatagtg cccagtcggc ttcgccggtt gaagccgacc
    11041 agccagtgta gaacatccgc acgccactct ctttttgccc tttaccttca acttctgccg
    11101 cccgctgtcc ggcatccatc gcggtcacct gggctttaat cccgacctgc gctaactgct
    11161 gctgggtaaa ttgcagcact ttctgcgcgg tgctgtggtt atgtgacgac cacagcgtgg
    11221 tactgaaacc gttgggatat cccgcctctt tcagtaattc gcgcgctttc actggatcgt
    11281 aaggccacgg tttataactt tgcgcgtagg cgatacttgg cggtaccaca ccagtagctg
    11341 gagttgcata gcccgcaaag gcaactttca ccagcgccgg acggttaatg gcgtaattca
    11401 gcgcctcacg gaccttcggg ttatcgaacg gcttttgcgt cacgttcata ctgatataac
    11461 gctgcataat tgacggactg gccatcaact cgatattttt gtttttctcc agcagtgtgg
    11521 cctgctcgta gggaatgggg aaagcaaact gcgcttcacc ggtttgcagc attgccgcgc
    11581 gggtgttgtt atccgccacc ggacgccagg ttatgctgtc cagtttgggc aatcctggct
    11641 gccagtaacc cgcgaatttt ttcaccttca caaaatcggt ctgattccag gtatccagtt
    11701 catacggtcc ggttcccacc ggatgaaaac caatctcctt gccatatttt tccagcgctg
    11761 tcggtgaaat catcgcggtc gccggatggg caagaatatt aataaacgct gagaacggct
    11821 gtttgagggt aatctttacc gttgtcggat cgatcgcctc cgttttggca atattcttat
    11881 acaggttata gcgtttaaga tgattcgccg gatcgctggc ccggtccaga ttcgctttta
    11941 ccgccactgc gttgaaatcg gtgccatcct ggaatttaat tccttcccgc aatttcacgg
    12001 tgtaagtaat gccgtcatcg gaaacggtat aactctccgc cagcacgttt ttcagtttca
    12061 tctctttatc cagaccgaac agcccctggt aaaacgattt cgctacggcc tgagataacg
    12121 tgtcattcgc gtcatacgga tcgagcgtgg tgaaattcga tcctaccgcc accaccacat
    12181 ctttggcagc gaatgcacaa gatgccatca acgctgtcgc aatgcccagc gccactaacc
    12241 cactacggtg tacagctctt gccattttat tctccgcctg aatgttatct acgcatgaat
    12301 gcgtattctg attgtggttg tgcgacgtaa tgccccggcc cgacgcattg caacgagacg
    12361 gctgccacct cttcgccacg cagatgaata ttgctgggaa gatcgtccga cagcagtaca
    12421 cgctgcggtc gttgtcggga cggttcagcg accggaactg ccgccagtaa tttacgcgta
    12481 taaggatgct gcgggttttc gaagaccgcg caccgtgaac caatttcaac aatttgcccg
    12541 agatacatca ccgccacacg atgactaatc cgctctacca cagccatatc gtgggagata
    12601 aacagatacg caatgccgaa atcacgctgg agatcgagca acaagttgat aatctgcccg
    12661 cgaatagaaa catccagcgc cgaaacggct tcgtcggcaa tgatcacttt tggattcaat
    12721 gccaacgcgc gagcaatgca gatgcgctgg cgctgaccgc cggaaaactc atgcgggtaa
    12781 cgccaggcat gttcaggtaa caggcccacg cgctccagca accacgcaac gcgtgcagcc
    12841 gcgtctttac ctggcaataa accgtgtaca cgcagcggtt cgataatcga atcaccgatg
    12901 gtctgacgtg ggtccagcga agcgtaaggg tcctgaaaaa taaactgaat atcccggcgc
    12961 aatgcctgaa gtttgccagg tgacaaggta tcgattcgct gaccgttaaa gataatttcg
    13021 ccgccctgcg attcgaccag gcgcagcaac gcccgcccgg tagtggattt accgctgcca
    13081 gactcgccca ccagcgatag cgtttcgcca ggccagagat caaaactgac tttctcaacg
    13141 gcatgcactt cccgcgttac gcgattcaac aaaccgctgc gcaaagggaa acgggtgaca
    13201 agattacgca cccgtaaaac aggttcgcca tccaccaccg ttttctgctc gatggggggt
    13261 tcctgtttcg ctggatgttc aagcgatatc aatgggaaac gtcggggata atctaaccct
    13321 ttcatcgcac caagttgcgg aacagcagct aacagcgcac gggtgtaagg atgttgcggt
    13381 gcatgaaaaa tctgttcgac ggtacccgtt tccaccgcct cgccctgata catcaccagt
    13441 acccgatcgg caatctctgc caccacgccc atatcgtgag tgataaagat aacgcccatc
    13501 gacatctctt tttgcaatac tttgattaat tgcaggatct gcgcctgaat agtgacatcc
    13561 agcgcggtgg ttggctcatc ggcaatcagc accgccgggc ggcatgacag cgccatcgca
    13621 atcatcactc gctggcgcat cccgccagag agttgatgcg aataacgtga aagaatggtt
    13681 tgtgcctccg gaatgcgtac ctgatccagc atccgcttcg cctcgaccat cgcttcttca
    13741 cgactggcgt tctgatgcag acgaattgat tcggcaatct gttcacccac agtaaatact
    13801 gggttcagcg atgtcatcgg ctcctgaaat atcatcgcca tatccgcacc gcgcacatgg
    13861 cgcatttgtg cagcgctctg ctcgctaagt tcaatcacat cacgactgcg ccgccgcaac
    13921 agcattttat cgcactgtac taaaccgccc gcctgttcca acagacgcat caatgccagc
    13981 gcagtcactg acttaccgga gccggattcg cctacaattg ccagcgtctc accgcgttgc
    14041 agactaaaag agagattgcg gaccgcagct attttctgct ggtcctgcat aaaggcaata
    14101 ttaagatttt caaccgccag cacattaccg gcatcaagtt catcactgtg tggcaacggt
    14161 gtcccctttt tcacggtaga taccggtggt tggcgtatcg cctgcgtagc cccaggcgcg
    14221 atacattcct tcggtgttaa acggtagcgc gacattccct tcatggtcga tagcgattaa
    14281 gccaccgcta ccgccaagcg cagggagttt ttccattact acccgctcgc aggcttccgc
    14341 gagacttaat ccgccgtaat ccattaacgc ggcgatgtca tatgccgcca gcgcgcggat
    14401 gaagacttcg cctgtgccgg tacaagaaac cgccacactg gcgttattgg cgtagcatcc
    14461 ggcacccact aagggactat cgccaactcg tccgggtaat ttattggtca ttccgcctgt
    14521 ggacgtggct gccgccagat tgccgtctaa atccaacgcc acggccccca cggtgcccat
    14581 tttctgtttt tcatccagtg gcgcaccgct atggtcgagg actgttgccc cttcctcgcg
    14641 cgctgccagt agttgttcat aacgcaaagg cgtggagaaa atctccggtg agacgcgctc
    14701 catgccatga gcaaacgcaa aattttctgc cccttcgcca atcatcatca catgcgggct
    14761 ttgctccatc accagccggg cggcaagaac cggattacgc agatgactaa cgcccgccac
    14821 cgcaccggct ttcagggtgt taccatccat cacacaggcg tccagttcat gggtttcatc
    14881 acgcgtaaag acagcgccaa ttccggcgtt aaacagtgga cactcttcca gcagacgcac
    14941 cgcttccgtc accacatcca gcgcactttc gccggcttcc agcattttct gcccggtttc
    15001 aacaatggca gacaacgcct cgatgtagcg taattcctgt tgcagactca tctgcgcgcg
    15061 gctaattgca cctgcgccac catgaattgc aatgactgct ttgcccatag tattcgtcca
    15121 ttaagggtgg ctcgccactt ttgctataaa tatcagaatt tttatgaatt actcattcaa
    15181 tttttgaata tagaaagatg gatatggcat gtaaaggcag gagccaacgc tacgcccgag
    15241 gtggcataca gtctatggcg tttttctgac ataataggca aattcgattt tgcctccgca
    15301 ggagtgtttt catggaattt accaccggat tgatgtcgct cgacaccgcg cttaatgaga
    15361 tgctttctcg cgttacccca ctgaccaccc aggaaacgct gccactggtt cagtgttttg
    15421 gtcgtattct ggcgagcgat gtcgtttcgc cactggatgt cccggggttt gataactccg
    15481 ctatggacgg ctacgcggtg cgtttagccg atattgcctc cgggcaaccg ctgcccgttg
    15541 ccggtaaatc ctttgccggt cagccatacc atggtgaatg gcctgcgggt acctgcattc
    15601 gtattatgac cggtgcgccg gtgccggaag gctgcgaagc ggtggtgatg caggagcaga
    15661 ctgaacaaac ggacaatggc gtgcgtttta ctgctgaagt gcgtagcggg caaaatattc
    15721 gccgtcgcgg tgaagatatc tctgcaggtg cggttgtttt cccggcggga actcgcctga
    15781 ctaccgcaga gctgccagta attgcttcac tggggattgc cgaagttccg gtgattcgta
    15841 aagtgcgtgt agcgcttttt tctaccggtg atgaactcca gttgcccggt cagccgctgg
    15901 gcgacggcca aatctacgac accaaccgtc tcgccgtaca cctgatgtta gaacagctgg
    15961 gttgcgaggt aatcaactta gggattatcc gcgacgatcc acatgccctg cgcgccgcat
    16021 ttattgaagc cgacagccag gcggatgtag tgatcagttc cggcggtgtt tcagtgggtg
    16081 aagcggatta caccaaaatg attcttgaag agctggggga gatcgccttc tggaagctgg
    16141 cgattaaacc aggtaaaccg ttcgcgttcg gtaaactcag caatagctgg ttctgcggcc
    16201 tgccgggcaa cccggtttca gcgacgctga ccttctatca actggtacag cctttgctgg
    16261 caaaactaag cggcaacacc gccagcggcc tgcccgcgcg ccagcgcgta cgcacagcgt
    16321 cccgcctgaa aaaaacgcca ggacgtcttg atttccagcg cggcgtgctg caacgcaacg
    16381 ccgacggtga acttgaagtg acgaccaccg gacatcaggg ttcacatata tttagctcct
    16441 ttagtctcgg caactgcttt atcgtgctgg aacgcgatcg cggcaatgtg gacgtgcgcg
    16501 aatgggtgga agtagaaccg tttaacgcgt tgttcggagg cctgtaatgg cggaactcag
    16561 cgatcaggag atgctgcgct acaaccggca aatcattctg cgcggttttg attttgacgg
    16621 tcaggaggcg ctaaaagatt ctcgtgtgct ggtagtcggc ctcggtggac tcggctgcgc
    16681 agcttcgcag tatctggcaa gcgccggtgt cggcaacctg acgctgctcg acttcgacac
    16741 ggtttcgctc tcgaatctgc aacgccagac actgcacagt gatgccacgg tcgggcaacc
    16801 gaaggtggaa tccgcccgtg acgccctgac gcggatcaac ccacatatcg cgattacgcc
    16861 agtcaatgca ctgctagatg acgcagaact tgcagcattg attgctgaac acgatctggt
    16921 gctcgactgt acggataacg ttgcggtacg taatcaactg aacgcaggct gttttgccgc
    16981 gaaggtgccg ctggtttccg gcgcggcaat tcgtatggaa gggcaaatca cggtcttcac
    17041 ttatcaggac ggcgaaccgt gctatcgctg ccttagccgt ttgtttggcg agaacgcgtt
    17101 aacctgcgta gaagcaggcg tgatggcacc gttgataggc gtaattggtt cgttgcaggc
    17161 aatggaagcg atcaaactgc tggcaggtta tggcaaaccg gccagcggga aaatcgtcat
    17221 gtacgatgcg atgacctgtc agtttcgcga aatgaaactg atgcgcaatc cggggtgtga
    17281 ggtgtgcggg cagtaattgc cacctccaag cctgataaga cgcgacaagc gtcgcatcag
    17341 gcatttgctc cagacgccag atgcggcgtg aacgccttat ctggcctacg gttcgggcac
    17401 agatttccaa gcgcggcagg catcggtgca ctccgccacg tcatcagcaa tttatgagga
    17461 tgcagaacgt ccggttaaat cgacgttctg ccaaacgctc cctgccagtc ctgctcaaac
    17521 ttcgtcacag cggcttccac cgccggatag ctaatcatct gttgtgccac atccagtggc
    17581 agagtaattg attcacatcc ggccagtaag cagtccagcg cctgacgcgg ggttttgaaa
    17641 ctcgctgcca gcactttcgc ctgcggcgca tgcattttca ataactggtg taagtcggtc
    17701 acagtctgaa tgccgctacc gccctgagca tcaatacgat taacgtaagg cgcaacatat
    17761 tccgcacctg ccagcgccga cagcagccct tgtgccgcgc catataccgc ggttcccagc
    17821 gtcggaatcc cttccgcttt taacatctta atcgctgcca gcccctcggc tgtcaccgga
    17881 actttcacca cgatatccgc aataatagaa cgcagcttaa gcgcgtcatt caccatccct
    17941 tcagcagtgg tagccattac ctgggcaaac agacgcccct gaccgcccat cgcttcatga
    18001 agttgcggaa gcacaacatc cagcggtttt ttacccgcgg cgataatgct tgggttagtg
    18061 gtcacacccg ccagcggaaa aatacgtgac agcgccttca ccgcaacaac gtctgaagta
    18121 tccagataca gttccataac catcctcaaa atgtactttt tgtctgaatg tagactatca
    18181 cgggaaaact ctctgaacag ttgacgcaca tcaatctaac tttcattcga aagtatttta
    18241 atgtttgaac gaaacagaga ggcaactatg attttcaata ttcagcgcta ctcgacccat
    18301 gatggccccg gtatccgcac ggtcgtattt cttaaaggct gttcgctggg ctgccgctgg
    18361 tgtcagaacc cggaaagccg cgcccgtacg caggatctgc tgtatgacgc acgactgtgt
    18421 ctggaaggct gcgagctgtg cgctaaggcc gcgccggaag tgattgagcg cgcgctgaat
    18481 ggtttgctta ttcatcggga aaagttaacc ccggagcatc tgacggcgtt aaccgactgc
    18541 tgtccgacac aggcgttaac cgtgtgtggt gaagtgaaaa gcgttgagga gatcatggcg
    18601 accgttctgc gcgataaacc gttttacgat cgcagcggcg gcggcttaac gctttcgggt
    18661 ggtgaaccct tcatgcagcc ggaaatggcg acggcgctgc tgcaagcctg ccacgaggca
    18721 ggcattcata ctgcggtaga aacctgtctg catgtgccgt ggaaatatat cgccccttct
    18781 ctgccctata tcgatctgtt tcttgccgat ttaaaacacg ttgccgacgc gccgtttaaa
    18841 cagtggaccg acggtaacgc cgccagggtg ctggataacc tgaaaaaact cgccgcagcg
    18901 ggcaaaaaaa tcattatccg cgtgccgctg attcagggct ttaatgccga cgaaacctct
    18961 gtaaaagcca ttaccgattt tgccgccgac gagctgcacg ttggcgaaat tcattttctg
    19021 ccctaccaca cgctgggcat caacaaatat cacttactta atctgcccta tgacgccccg
    19081 gaaaaaccgc ttgatgcgcc agagctgctc gattttgccc agcagtatgc ctgccagaaa
    19141 gggttaaccg cgaccttacg aggataacaa ccatgaccac actgaaactg gacacgctca
    19201 gcgaccgcat taaagcgcac aaaaatgcgc tggtgcatat tgtgaagccg ccagtctgta
    19261 ccgagcgcgc gcagcactat accgagatgt atcaacaaca tctcgataag ccgatcccgg
    19321 tacgtcgcgc gctggcactg gcgcatcacc tggcgaatcg caccatctgg atcaaacacg
    19381 atgagttgat cattggcaac caggcaagcg aagttcgcgc cgcgccgatc ttcccggaat
    19441 atactgtctc gtggatcgaa aaagagattg atgatctggc agatcgtccc ggtgctggct
    19501 ttgcggtgag cgaagagaac aaacgtgttc tgcatgaagt gtgcccgtgg tggcgcggtc
    19561 agacggtaca ggatcgctgc tatggcatgt ttaccgatga gcaaaaaggt ctgctggcga
    19621 ccggcatcat taaagcggaa ggcaatatga cctccggcga tgcgcacctg gcggtcaatt
    19681 tcccgctgct gctggaaaaa gggcttgatg gtctgcgtga gaaagtagcg gaacgtcgct
    19741 cgcgcatcaa tctgacggtg ctggaagatt tgcacggcga acagttcctg aaagcgattg
    19801 atatcatgct ggtggcagtc agtgaacaca ttgaacgttt cgctgccctg gcgcgtgcaa
    19861 tggccgcgac cgaaacccgc gaaagccgtc gcgatgaact gctggcgatg gcagaaaact
    19921 gcgatcttat cgcccaccag ccgccgcaga ctttctggca ggcgctgcaa ctgtgttact
    19981 tcatccagtt gattttgcag attgaatcta acggtcactc ggtgtcgttt ggtcgtatgg
    20041 atcagtatct ctacccgtac tatcgccgcg acgttgaact gaaccagacg ctggatcgcg
    20101 aacacgccat cgaattgctg catagctgct ggttgaaatt gctggaagtg aacaaaatcc
    20161 gctccggctc ccactcaaaa gcctctgcgg gaagtccgct gtatcagaac gtcaccattg
    20221 gcggacaaaa tctggttgat ggtcaaccaa tggacgcggt gaatccactc tcttacgcga
    20281 tcctcgaatc ctgcggtcgc ctgcgttcca ctcagcctaa cctcagcgtg cgttaccatg
    20341 caggaatgag taacgatttc ctcgacgcct gcgtacatgt gatccgctgc ggcttcggga
    20401 tgccggcatt caacaacgac gaaatcgtga tcccggaatt tattaaactc ggtattgaac
    20461 cgcaggacgc ttatgactac gcagcgattg gttgtataga aaccgccgtc ggtggcaaat
    20521 ggggctatcg ctgtaccggc atgagcttta tcaacttcgc ccgcgtgatg ctggcggcgc
    20581 tggaaggcgg gcgtgatgcc accagcggca aagtgttcct gccacaagaa aaagcgttgt
    20641 cggcaggtaa cttcaacaac ttcgatgaag tgatggacgc gtgggatacg caaatccgtt
    20701 actacacgcg taaatcaatc gaaatcgaat atgtcgtcga caccatgctg gaagagaacg
    20761 tgcacgatat tctctgctcg gcgctggtgg atgactgtat tgagcgagcg aaaagtatca
    20821 agcaaggcgg cgcgaaatat gactgggttt ctggcctgca ggtcggtatt gccaacctcg
    20881 gcaacagcct ggcggcagtg aagaaactgg tgtttgaaca gggcgcgatt ggtcagcaac
    20941 agctggctgc cgcactggcg gatgacttcg atggcctgac tcatgagcaa ctgcgtcagc
    21001 ggctgattaa cggtgcgccg aagtacggca acgacgatga taccgtcgat acgctgctgg
    21061 ctcgcgctta tcagacctat atcgacgaac tgaaacagta ccataaccca cgctacggtc
    21121 gtggtccggt tggcggcaac tattacgcgg gtacgtcgtc tatctccgct aacgtaccgt
    21181 ttggcgcgca aactatggca acgccggacg ggcgtaaagc ccacaccccg ctggcagaag
    21241 gcgctagtcc ggcctctggt actgaccatc tcggccctac tgcggtcatt ggctcagtgg
    21301 gtaaactgcc tacggcagcg attctcggcg gcgtgttgct caaccagaaa ctgaatccgg
    21361 caacgctgga gaacgaatct gacaagcaga aactgatgat cctgctgcgt accttctttg
    21421 aagtgcataa aggctggcat attcagtaca acatcgtttc ccgcgaaacg ctgctggagg
    21481 cgaaaaaaca tccggatcag taccgcgatc tggtagtgcg tgtcgcgggt tattccgcct
    21541 tcttcaccgc gctctctcca gacgctcagg acgatatcat cgcccgtact gaacatatgc
    21601 tgtaatctct tcggcccggc tgcggcgtcg ccgggctaaa tcattcacat catcaatttc
    21661 atccttactt tcattcgaaa tataatttgt gctctgcgtc acattgttat tacactgtta
    21721 cggtttcatt ttcgagccag gagcacacct atgagcgtaa aagttatcgt cacagatatg
    21781 gacggtactt ttcttaacga cgccaaaacg tacaaccaac cacgttttat ggcgcaatat
    21841 caggaactga aaaagcgcgg cattaagttc gttgtcgcca gcggtaatca gtattaccag
    21901 cttatttcgt tctttcctga gctaaaggat gagatctctt ttgtcgcgga aaacggcgca
    21961 ctggtttacg aacatggcaa gcaactgttc cacggcgaac tgacccgaca tgaatcgcgg
    22021 attgttattg gcgagttgct aaaagataag caactcaatt ttgtcgcctg cggtctgcaa
    22081 agtgcatatg tcagcgaaaa tgcccccgaa gcatttgtcg cactgatggc aaaacactac
    22141 catcgcctga aacctgtaaa agattatcag gagattgacg acgtactgtt caagttttcg
    22201 ctcaacctgc cggatgaaca aatcccgtta gtgatcgaca aactacacat agcgctggat
    22261 ggcattatga aacccgtcac cagtggtttt ggctttatcg acctgattat tcccggtcta
    22321 cataaagcaa acggtatttc gcggttactg aaacgctggg atctgtcacc gcaaaatgtg
    22381 gtagcgattg gcgacagcgg taacgatgcg gagatgctga aaatggcgcg ttattccttt
    22441 gcgatgggca atgctgcgga aaacattaaa caaatcgccc gttacgctac cgatgataat
    22501 aatcatgaag gcgcgctgaa tgtgattcag gctgtgctgg ataacacatc cccttttaac
    22561 agctgacctc tctaccggag ccatgcgctc cggttttccc ttctttttac atcctgtaca
    22621 tcacatcaaa tcattaaact tgcattaact tatttataac ttgtattgtc acatgcaaat
    22681 tagcatagtt tcgaatgaaa gttaatgcaa ggtggttatg gcttctactt ttaccagcga
    22741 cacattgcct gccgatcaca aagcagctat ccgtcagatg aagcacgcgc tgcgggcgca
    22801 gcttggcgat gtccagcaga tctttaatca gctaagcgat gacattgcca cgcgagtggc
    22861 tgaaatcaac gcactcaaag cacagggcga tgccgtctgg ccggtgctgt cttatgccga
    22921 tatcaaagca ggtcatgtta ctgcagagca gcgcgaacag attaaacgtc gcggttgtgc
    22981 ggtgataaaa ggccatttcc cccgcgaaca agcgctaggc tgggatcagt cgatgctgga
    23041 ctatctgaac cgcaaccgct ttgacgaggt ctacaaaggc cccggcgata atttcttcgg
    23101 gacgctcagc gcttcacgtc ccgagattta ccccatctac tggtcgcagg cgcaaatgca
    23161 ggcccgccag agtgaagaaa tggcgaatgc gcagtcgttt ctcaatcgtc tgtggacatt
    23221 tgaaagtgat ggaaagcaat ggtttaaccc ggatgtgagc gtcatctacc ctgaccgtat
    23281 ccgccgcctt ccgcccggaa cgacctccaa aggtcttgga gcgcataccg actccggggc
    23341 gctggaacgc tggctgcttc cagcgtatca gcacgttttc gccaacgtct ttaatggcaa
    23401 tctggcgaaa tacgatccct ggcatgcggc acatcgtacg gaagttgaag agtacacggt
    23461 ggacaacacc accaaatgtt ccgtgtttcg gacattccag ggctggactg cgctctcgga
    23521 tatgctgcct ggtcaggggt tgctgcacgt cgtgcccatt cctgaagcca tggcgtacgt
    23581 actgttacgt ccgctgcttg atgatgtgcc ggaggatgaa ctgtgcggcg tagcgcccgg
    23641 aagagtgttg ccggtatcag agcaatggca tccactgtta attgaggcgt taaccagcat
    23701 tccaaaactc gaggccggag actccgtctg gtggcactgc gacgtcatcc attccgttgc
    23761 ccccgttgaa aatcaacagg gttggggcaa cgtgatgtac attcctgcgg caccgatgtg
    23821 cgagaaaaat cttgcctacg cgcacaaggt gaaggccgca ctggaaaaag gcgcatcgcc
    23881 gggcgacttc ccgcgcgagg actatgaaac aaactgggaa ggacgcttta cgcttgccga
    23941 cctcaacatt cacggtaagc gagcgttggg catggatgtt tgagagttaa tggaagtgaa
    24001 ttgatgtggg attaagaaaa tcgattcgac gttactcaga ttgctgacaa gtgcgctttc
    24061 ttaatgcctg atgcggcgtt aacgccttat ccggcctaca aagtcctgca gattcaatat
    24121 attacaggta ctgcgtaggc ctgataagcg tagcgcatca ggctattttg catttgtcat
    24181 cagccgtcgt caccccgaca gtatcggggt gacggataat cacttactcg atccctttac
    24241 tacgcaggta atcttcgtaa ttaccgctaa agtcgatcac gcgttccggg gtgatttcca
    24301 gaatgcgggt cgccagggag cttacgaact cacggtcgtg tgaaacaaag atcagcgtgc
    24361 cctgatacag ttccagcgcc atgttcagtg actcgatgga ttccatatcc aggtggttgg
    24421 tcggttcgtc catgatcaga atgttcggct tctgcatcat taacttacca aacagcatcc
    24481 gccctttttc cccaccggaa agcactttag ctggcttttt gatgtcgtcc tggctgaaca
    24541 gcaaacgacc gagaatgctg cgtaccgcct gctcgtcatc gccttcctgc ttccactgac
    24601 tcatccattc gaacacggtc agatcatttt caaactcata ttcgtggtcc tgggcgtagt
    24661 aaccaatgcg cgcgttttca gaccatttta cggtgccgct gtccggttgc agatcgccca
    24721 ccagcgtttt cagcagcgtt gatttaccga cgccgttggt acccagtacc gcaagttttt
    24781 caccgacctc cagcagcagg ttgagatttt taaacagtgg accgttatca aaccctttgg
    24841 tcagaccttc cacttccagc gcgttacgga acagtttctt atcctgttca aaacggataa
    24901 acgggttctg acggctggag gctttcacct cttccagttt gattttatca atctggcgcg
    24961 cgcgcgaagt tgcctggcga gatttcgagg cgttggcgct aaagcggcta acgaaagatt
    25021 gcaactcagc aatctgcgct ttcttcttgg cgttatcggc cagcagacgt tcacgcgcct
    25081 gggtcgccgc cgtcatgtac tcatcgtagt tacccggata aacgcgcagc tcgccgtaat
    25141 ccagatccgc catgtgggta cagaccatgt taaggaagtg acggtcgtgc gagatgatga
    25201 tcatggtgct gtcacgctcg ttcagcacct gttccagcca gcgaatggtg tcgatgtcga
    25261 ggttgttggt cggttcgtcg agcaggagaa tatccggctc agcaaacagc gcctgcgcca
    25321 gaagcacacg cagcttccag ccaggagcaa cttcactcat cgggccgtag tgctgttcca
    25381 ctggaattcc cacgccaagc aacagttcac cggcgcgagc ttccgcagag taaccgtcca
    25441 tttcgccgta tttcacttcc agatcggcca ctttatagcc gtcttcttca ctcatttccg
    25501 gcaaagcata gatgcggtcg cgctcctgct tcacttccca caactcttta tgccccatga
    25561 tcaccgtatc cagcacagtg aactcttcaa aggcaaactg atcctgacgc agcttaccaa
    25621 tgcgctcgtt gggatcgagg gaaacgttac ccagcgtcgg ctcaaggtcg ccgccgagaa
    25681 tcttcataaa ggtggattta ccactaccgt tcgcgccaat caggccgtaa cggttgccgc
    25741 cgccgaattt gacggaaatg ttttcaaaca acggcttact gccgaactgc atggtgacgt
    25801 tactggaaac taacacaggc gtatcctgaa aagagatatg acaaaccgcg cattatgcca
    25861 taagtctgaa agaagatcct gctcctcgct taaggtctac actcaagcca aaggcaaaaa
    25921 atgtgatttc gtacacatct gatttcactg tgagctggaa tgaacttata atgcgcttcc
    25981 aatactctaa tattctcaac ccaatggcct gccaggcaca aaatctcgct taacatgaat
    26041 atgaaattga aaacattatt cgcagcggcc ttcgctgttg tcggcttttg cagtaccgcc
    26101 tctgcggtaa cttatcctct gccaaccgac gggagtcgcc tggttggtca gaatcaggtg
    26161 atcaccattc ctgaaggtaa cactcagccg ctggagtatt ttgccgcgga gtaccagatg
    26221 gggctttcca atatgatgga agcgaacccg ggtgtggata ccttcctgcc gaaaggcggt
    26281 actgtactga acattccgca gcagctgatc ctgccggata ccgttcatga aggcatcgtc
    26341 attaacagtg cagagatgcg tctgtattac tatccgaaag ggaccaacac cgttatcgtg
    26401 ctgccgatcg gcattggtca gttaggcaaa gatacgccta tcaactggac caccaaagtt
    26461 gagcataaga aagcaggccc gacctggacg ccgaccgcca aaatgcacgc agagtaccgc
    26521 gctgcgggcg aaccgctacc ggctgtcgtt ccggcaggtc cggataaccc gatgggtctg
    26581 tatgcactct acatcggtcg cctgtatgct atccatggca ccaacgccaa cttcggtatc
    26641 ggcctgcgtg taagtcatgg ttgtgtgcgt ctgcgtaacg aagacatcaa attcctgttc
    26701 gagaaagtac cggtcggtac ccgcgtacag tttattgatg agccggtaaa agcgaccacc
    26761 gagccagacg gcagccgtta tattgaagtc cataacccgc tgtctaccac cgaagcccag
    26821 tttgaaggtc aggaaatcgt gccaattacc ctgacgaaga gcgtgcagac agtgaccggt
    26881 cagccagatg ttgaccaggt tgttcttgat gaagcgatca aaaatcgttc cgggatgccg
    26941 gttcgcctga attaatcgtc aaaacgtaaa acaaaggcgg actcataatc cgcctttttt
    27001 atttgccaga ccttaccagg ccgggatcat aactaacaaa acgtatccca ccagcgccgc
    27061 ccacaacagc atcggtattg aatagagatg gaaacgccac cagatgcggc gatcgttcgc
    27121 catacgtagc gcaatcaaat ttgccagcga tcctggtaat aaaccaaagc cccccacgtt
    27181 taccgcccat gccagtaata aagacggagg tacataattg agcagcaaaa tggtactcgg
    27241 cacgttactg atcacctgcg ataaaccgac tgccgttaac cataacccgg gttcagataa
    27301 atgactcacg ttacccaaca cgccttgcaa cgctggaagc tgggtcagta aatggacgtc
    27361 gataaacatc gccataaaca ccagcagcaa cgtccagtcc acactcaaca ccacgcgacg
    27421 cgccagcagc acaaagcccg ccgccacaat caccagtccc cacagctctt gtttgaactc
    27481 cagcgccgtc agaaagacga tatacagccc cagacaattc cacaccagcc gcggtttcca
    27541 ctccggtgtt tgcacccccg tatggtattg cagcgccttt ccagggaaac aacaccagca
    27601 caacagcagg agtgtcagca tcattgcgcc agccatcggt gccatttggg caataaatcc
    27661 ggcaaacgaa agaccagaac gtccccagat aagaatattt tgcgggttgc caattggcgt
    27721 cagtagcgaa ccagcgttga ctgccagcgc ctcaaaaata atcagccgat taaccgggat
    27781 ctcacacagt ctttttagcg tgatagtcag cggaacaaca ataaacagcg cgacatcgtt
    27841 ggtcagaaag gtagaaagca gcgccgccgc cagcaccata aacatcgcca gccgacgctc
    27901 cgtagcaaag cggcgcacca ttttgcgccc cagcacatca aaataaccgc ttaactccac
    27961 acctttggtc agcagcatca ggccgcttaa ggtgatgatg gtgtgccagt cgatagcagc
    28021 aggccaggat ttcggtgcaa agggcacgaa aaagcttaat ccgataccaa caagaattaa
    28081 taactgaaaa aaacgatcgc cttgcagcgt gcgtaaaaaa ggcaggctca tttggcaccg
    28141 tgtttctggg taaacaaacg aaaagcgtcg agcgtctctt cactaacatg gtgctccatg
    28201 ccttccgcgt cgcgacgggc gatttccgga ctgacgccca acaccaacaa gaaattttcg
    28261 actatctgat gtcgctcgcg gctttcctgc gccagcttct ctccttctgc cgttaaaaac
    28321 acgccacgcc aggggatcat ttcaatcagc cccatggttg ccagccgctt aagcatttta
    28381 gccaccgtcg gttgcgaaac tcccagacga gcagccatgt ccacctgacg agcttccccc
    28441 acttccctga tcaagtcaga aatcagctca acgtaatcat caataagctc gcgccgatgc
    28501 gcctctctga cctggcggaa cccttcaacg tgctcttcca cgttcactaa ctgcgtcact
    28561 ttttttgcta ttggcgtacc tgcgcgacga ctcattgtgc ttcctcatta tggtgacgca
    28621 tacatagcgt tctcatttcg tgcgcacatt gtaaaccaga gttgcgaagg tacaaaaaat
    28681 taacgtttta gcaatagcta tataatatag cctgtgctat atctgtatgt aatgcaatca
    28741 tccctcaagg atcgacggga ttagcaagtc aggaggtctt atgaatgagt tcaagaggtg
    28801 tatgcgcgtg tttagtcatt ctccctttaa agtacggtta atgctgctct ctatgttgtg
    28861 cgatatggtc aacaacaaac cgcagcaaga taaaccttca gataaatagc ggcgtcgcgg
    28921 tacgccgctt cactcctgct tttatgcagg cataacgcgt tttggtctga aaaaaaccac
    28981 tttttgtcgg atttgcaatc cccttcgcaa aagatttgtt cgtcagtagt tgacctgaac
    29041 ggcggctcgc tctatcttct tgcagccctg cgtatattgc ggctcgcgga tgcggacccc
    29101 tttcccctct tcacgcactc ttgcaggtat tgacccttga cgccagggta agcacatggc
    29161 gtttgttacg atagtggcat atgaatttaa ccctcaaaga atcgcttgtt acccgtagcc
    29221 gggtatttag cccgtggact gcgttctact ttttacagtc gctattaatt aacctcggct
    29281 taggttaccc cttcagtttg ctctacaccg ctgcgtttac ggctattttg cttttgctat
    29341 ggcgaacatt gcctcgcgta caaaaagttc tggtcggtgt cagttcgctg gtggcggctt
    29401 gttatttccc ttttgctcag gcctacggcg cgcctaattt caatacattg ctggcattgc
    29461 actccaccaa tatggaagag tcgaccgaaa tcctgacgat ttttccgtgg tacagctacc
    29521 tggtcggctt atttattttt gcgctcggcg taatagcaat caggcgaaaa aaagagaatg
    29581 aaaaagcgcg ctggaatacc ttcgacagcc tgtgtctggt attcagtgtg gcgacatttt
    29641 ttgttgctcc cgtgcaaaac ctggcctggg gtggcgtatt taaactgaaa gatactggct
    29701 atccggtatt tcgttttgct aaggatgtca tcgtcaataa taacgaggtg attgaagagc
    29761 aagaacggat ggcaaaactt tccggaatga aagatacctg gacggtcact gccgttaagc
    29821 cgaagtatca gacctatgtg gtagtgatcg gtgaaagcgc gcgtcgcgat gccctcggtg
    29881 cctttggcgg tcactgggac aataccccgt ttgccagcag cgttaacggt ttgatatttg
    29941 ctgactacat tgctgccagt ggctccacgc agaaatcgct tggcttaacg ctcaatcgcg
    30001 tagtcgatgg caaaccacag tttcaggata actttgtcac cctggcaaat cgcgcgggct
    30061 tccagacctg gtggttttcc aaccagggcc aaatcggcga atacgatact gctatcgcca
    30121 gcatcgccaa acgtgcagat gaagtgtact tcctgaaaga aggtaatttt gaagcagata
    30181 aaaacacgaa agacgaagcg ttactggata tgaccgctca agtgctggcg caagagcact
    30241 cgcaaccgca gctgattgtt ctgcatctga tgggctcgca tccgcaggcc tgcgacagga
    30301 cacaaggcaa atacgaaacc tttgtgcaat cgaaagaaac gtcgtgctat ctctatacca
    30361 tgacgcaaac ggacgattta ctgcgcaagc tgtacgatca gttacgcaac agcggcagca
    30421 gcttctctct ggtttacttt tctgaccacg gtctggcctt taaagagcgc ggcaaagacg
    30481 tgcaatacct tgcccatgat gataaatatc agcaaaattt ccaggtgcct tttatggtca
    30541 tttccagcgg cgataaagcg catcgggtga ttaaagcccg ccgctcagcc aatgacttct
    30601 taggcttttt ctcgcagtgg acgggaatta aagcgaagga aataaatatc aaatacccgt
    30661 ttatatctga gaagaaagcc gggccgatat acatcaccaa cttccagtta cagaaggtgg
    30721 attacaacca tctcggaacc gatattttcg acccgaaacc ttaaaaacaa aaatccgccc
    30781 cgagaggcgg atttttatat caccaaagtg attagaagcg gtaaccaaca ccggcaatcc
    30841 aggtgcctac gtcaacgcta cgaatacggc tctgctcgta agagaagtcc agagcaacgt
    30901 tttccatcgg gttgaactgc agaccagcac cgtaggagaa accgtagtcg ctggtgtcgt
    30961 gtttgtaggt cgggtattca gtggtctgga atttaccata acccacaccc actacaccgt
    31021 agatgcttgc ccagtcgtta atgcggtaag ccggaccagc agtgatgccg tagtactggt
    31081 ttttgttgta gtcaccagag cttgcagtac ggcttttctc ggtgtaagtg aaagaaccga
    31141 tcacacccag cgggctgttg tcttcttcat agcggtattt caggttgaaa ccgcccattt
    31201 tgttcatttg gccctgagcg tcgctctgtg cgtaaccgcc agttacggta gaagtcgcag
    31261 ctacggaagt acctgcggtg aaagccagaa ctgcggccag tgctgaaaga catgcaattt
    31321 ttttcataac cacctcaaat gtgattcaaa taagtcctaa gttttaaata tatcaaaaat
    31381 taatgggaaa ctcttcgcga tttgtgatgt ctaacgggcc atttcatgta acagaacgtt
    31441 tccatacacc gctatccatc taaatttaaa tcactttttc agagaactgc gtaagtatta
    31501 cgcatgtttt ccctgtcatt catccagatt attcctaatc accagactaa tgattccatc
    31561 aatcctggcg cattttagtc aaaacggggg aaaatttttt caacaaatgc tcaaccagca
    31621 ttgggtatat ccagtacact ccacgcttta cttaagtcta gatatttgtg ggagaaagga
    31681 tgcctggttc attacgtaaa atgccggtct ggttaccaat agtcatattg ctcgttgcca
    31741 tggcgtctat tcagggtgga gcctcgttag ctaagtcact ttttcctctg gtgagcgcac
    31801 cgggtgtcac tgcgctgcgt ctggcattag gaacgctgat cctcatcgcg ttctttaagc
    31861 catggcgact gcgctttgcc aaagagcaac ggttaccgct gttgttttac ggcgtttcgc
    31921 tgggtgggat gaattatctt ttttatcttt ctattcagac agtaccgctg ggtattgcgg
    31981 tggcgctgga gttcaccgga ccactggcgg tggcgctgtt ctcttctcgt cgcccggtag
    32041 atttcgtctg ggttgtgctg gcggttcttg gtctgtggtt cctgctaccg ctggggcaag
    32101 acgtttccca tgtcgattta accggctgtg cgctggcact gggggccggg gcttgttggg
    32161 ctatttacat tttaagtggg caacgcgcag gagcggaaca tggccctgcg acggtagcaa
    32221 ttggttcgtt gattgcagcg ttaattttcg tgccaattgg agcgcttcag gctggtgaag
    32281 cactctggca ctggtcggtt attccattgg gtctggctgt cgttattctc tcgaccgctc
    32341 tgccttattc gctggaaatg attgccctca cccgtttgcc aacacggaca tttggtacgc
    32401 tgatgagcat ggaaccggcg ctggctgccg tttccgggat gattttcctc ggagaaacac
    32461 tgacacccat acagctgctg gcgctcggcg ctatcatcgc cgcttcaatg gggtctacgc
    32521 tgacagtacg caaagagagc aaaataaaag aattagacat taattaaatt tacatttctg
    32581 tatggttatg cataaccatg cagaatttct cgctactttt cctctacacc gtatttatat
    32641 atcgaattat gcaaaagcat atttattccg aaaattcctg gcgagcagat aaataagaat
    32701 tgttcttatc aatatatcta actcattgaa tctttattag ttttgttttt catgcttgtt
    32761 accactatta gtgtgatagg aacagccaga atagcggaac acatagccgg tgctatactt
    32821 agtctcgtta attactggga cataacatca agaggatatg aaattatgag taccgctaaa
    32881 ttagttaaat caaaagcgac caatctgctt tatacccgca acgatgtctc cgacagcgag
    32941 aaaaaagcaa cagtagagtt gctgaatcgc caggttatcc agtttattga tctttctttg
    33001 attaccaaac aagcgcactg gaacatgcgc ggcgctaact tcattgccgt acatgaaatg
    33061 ctggatggct tccgcaccgc actgatcgat catctggata ccatggcaga acgtgccgtg
    33121 cagctgggcg gtgtagctct ggggaccact caagttatca acagcaaaac cccgctgaaa
    33181 agttacccgc tggacatcca caacgttcag gatcacctga aagaactggc tgaccgttac
    33241 gcaatcgtcg ctaatgacgt acgcaaagcg attggcgaag cgaaagatga cgacaccgca
    33301 gatatcctga ccgccgcgtc tcgcgacctg gataaattcc tgtggtttat cgagtctaac
    33361 atcgaataaa tccatcgctg atggtgcaga actttagtac ccgataaaag cggcttcctg
    33421 acaggaggcc gttttgtttt gcagcccacc tcaacgcact tatttagtgc atccatctgc
    33481 tatccccagc tgattaagta aattttttgt atccacatca tcacacaatc gttacataaa
    33541 gattgttttt tcatcaggtt ttacgctaaa taatcactgt gttgagtgca caattttagc
    33601 gcaccagatt ggtgccccag aatggtgcat cttcagggta ttgccctata aatcgtgcat
    33661 cacgtttttg ccgcatctcg aaaaatcaat gagttgcaaa attggcacga ttttttcata
    33721 tatgtgaatg tcacgcaggg gatcgtcccg tggatagaaa aaaggaaatg ctatgaagtc
    33781 tgtattaaaa gtttcactgg ctgcactgac cctggctttt gcggtttctt ctcatgccgc
    33841 ggataaaaaa ttagttgtcg cgacggatac cgccttcgtt ccgtttgaat ttaaacaggg
    33901 cgataaatat gtgggctttg acgttgatct gtgggctgcc atcgctaaag agctgaagct
    33961 ggattacgaa ctgaagccga tggatttcag tgggatcatt ccggcactgc aaaccaaaaa
    34021 cgtcgatctg gcgctggcgg gcattaccat caccgacgag cgtaaaaaag cgatcgattt
    34081 ctctgacggc tactacaaaa gcggcctgtt agtgatggtg aaagctaaca ataacgatgt
    34141 gaaaagcgtg aaagatctcg acgggaaagt ggttgctgtg aagagcggta ctggctccgt
    34201 tgattacgcg aaagcaaaca tcaaaactaa agatctgcgt cagttcccga acatcgataa
    34261 cgcctatatg gaactgggca ccaaccgcgc agacgccgtt ctgcacgata cgccaaacat
    34321 tctgtacttc atcaaaaccg ccggtaacgg tcagttcaaa gcggtaggtg actctctgga
    34381 agcgcagcaa tacggtattg cgttcccgaa aggtagcgac gagctgcgtg acaaagtcaa
    34441 cggcgcgttg aaaaccctgc gcgagaacgg aacttacaac gaaatctaca aaaaatggtt
    34501 cagtactgaa ccgaaataat aacgctacac ctgtaaagcg cactggcagt tccctctccc
    34561 ccctggggag aggattaggg tgaggggcgc aaacccgctc cggggccatt aattaccctg
    34621 aatttgatta tttacaccac ggtaacagga acaacatatg cagtttgact ggagtgccat
    34681 ctggcctgcc attccgcttc tgattgaagg tgccaaaatg accctgtgga tttcggtcct
    34741 cggtctggct ggcggtctgg taatcggatt gctggcaggt tttgcacgca ccttcggtgg
    34801 ttggatagcc aaccacgtcg cgctggtctt tattgaagtg atccgcggca cacctatcgt
    34861 cgtccaggtg atgtttatct atttcgccct gccgatggcg tttaacgact tacgcatcga
    34921 cccatttact gcggcggtgg tcaccatcat gatcaactcc ggcgcgtaca ttgcggaaat
    34981 cacgcgtggt gcggtgctgt ctatccacaa aggttttcgt gaagcaggac tggcgctcgg
    35041 tctttcacgt tgggaaacca ttcgctacgt cattttaccg ctggcactgc gtcgtatgct
    35101 gccgccgctg ggtaaccagt ggatcatcag cattaaagac acctcgctgt ttattgttat
    35161 cggcgtggcg gaactgaccc gtcaggggca agaaattatt gccggtaact tccgcgccct
    35221 tgagatctgg agcgccgtgg cggtgttcta tctgattatt accctggtgc tgagctttat
    35281 tctgcgtcgt ctggaaagaa ggatgaaaat cctgtgattg aatttaaaaa cgtctccaag
    35341 cactttggcc caacccaggt gctgcacaat atcgatttga acattgccca gggcgaagtc
    35401 gtggtgatta tcgggccgtc cggttccggt aaatcgaccc tgctgcgctg catcaacaaa
    35461 ctggaagaaa tcacctccgg cgatctgatt gtcgatggcc tgaaggtcaa cgatccgaaa
    35521 gttgacgagc gcctgattcg ccaggaagca ggtatggtgt tccagcagtt ttacctcttc
    35581 ccgcatctga cggcgctggg aaacgtcatg tttggtccgc tacgcgtgcg tggcgcgaac
    35641 aaagaagagg cggaaaaact ggcgcgtgag ctgctggcga aagtcggtct ggcagaacgt
    35701 gcacatcact acccttccga actttctggt ggtcaacagc agcgtgtggc gattgcccgc
    35761 gcgctggcgg tgaagccgaa aatgatgctg tttgatgaac cgacttccgc tcttgacccg
    35821 gaactgcgcc atgaagtgct gaaggttatg caggatctgg ctgaagaagg gatgacgatg
    35881 gtgatcgtga cccacgaaat cggttttgcc gagaaagtag cttcgcggct gatctttatc
    35941 gacaaaggcc ggattgcgga agatggcaat ccgcaggtgt tgatcaagaa cccgccgagc
    36001 cagcgcttgc aggaattttt gcagcacgtc tcttaataag acacattgcc tgatgcgcta
    36061 cgcttatcag gcctacagga tatctggcaa cttattaaaa ttgcatgaac ttgtaggacg
    36121 gataaggcgt tcacgccgca tccggcaaaa aagcccgcac gttgtcagca acctgcttaa
    36181 tatcccttcc tccctttcac ccgaaaggga ggcacaccag attcctctca tttaaaatcg
    36241 cccctccccc agcatctata cttatctttt tgctctgttt tctcactgga ggagtcatgc
    36301 ggtggatcct gttcatcctc ttctgcctgc tgggcgcacc tgcccacgcg gtatccatac
    36361 ccggcgttac aaccacaacg acaacggact caacgactga accggccccg gaaccggata
    36421 tcgaacaaaa aaagcggcct atggcgcact ggcggatgtg ctggataacg acacctcgcg
    36481 taaagagttg atcgaccagt tgcgtactgt cgccgcgacg ccccctgcgg aaccggtccc
    36541 gaagatcgtg ccgccgacgc tggttgaaga gcaaaccgtg ctgcaaaagg tcaccgaagt
    36601 cagccgccat tatggtgaag ccctttccgc ccgcttcggg caactttatc gcaatatcac
    36661 cggctccccg cataagccat ttaatccaca aaccttcagc aatgcgctga gccatttttc
    36721 aatgttagcg gtattagtgt ttggttttta ctggctgatt cgcctgtgcg cactgccgct
    36781 atatcgcaaa atgggtcagt gggcgaggca aaaaaatcgt gagcgcagca actggttgca
    36841 gcttccggcg atgattattg gggcgtttat tatcgacctg ctgttactgg ccctgacatt
    36901 atttgtcggc caggtattaa gcgacaacct gaatgcaggc agtcgcacca tcgctttcca
    36961 acaaagtttg tttctcaacg cctttgccct gattgaattt ttcaaagccg tactacgcct
    37021 gattttttgc ccaaacgtgg cggagctgcg cccgttcacg attcaggacg agagcgcccg
    37081 ttactggggt cgtcgcctga gctggttaag cagcctgatt ggctatggcc tgattgtggc
    37141 cgtgccgatt atctctaatc aggtgaatgt acagataggt gcgctggcga acgtcatcat
    37201 tatgctgtgc atgaccgtct gggcgttgta cctgatcttc cgaaataaaa aagagattac
    37261 ccagcatatg ctcaactttg cggagcattc gctggctttt ttcagcctgt ttatccgcgc
    37321 ctttgcgctg gtgtggcact ggctggcaag cgcctatttt atcatgctgt ttttcttttc
    37381 gttgttcgat ccgggcaaca gtctgaaatt tatgatgggt gcaacggtgc gcagcctggc
    37441 gattattggt atcgcagcgt ttgtttccgg tatgttttcc cgctggctgg cgaaaaccat
    37501 caccctctcg ccacatactc agcgtaacta tccggagcta caaaaacggc tgaatggctg
    37561 gctttcggcg gcgctgaaaa cggcgcgtat tctgaccgtc tgcgtggcag taatgttgct
    37621 gttgagcgca tggggattgt tcgatttctg gaactggctg caaaacggcg cggggcagaa
    37681 aaccgtagat atcctgatcc gcatcgcact cattcttttc ttctcggcgg ttggctggac
    37741 ggtgctcgcc agtttgatcg aaaaccggct ggcttcggat attcatggcc gcccgctacc
    37801 cagcgcccgt acgcgtaccc tgctgacgct gttccgtaac gcgctggcgg tgattatcag
    37861 taccatcacc atcatgattg tgttgtcgga aatcggcgtc aatatcgcgc cattgctggc
    37921 aggtgccggg gcattaggtc tggctatctc gtttggttcg caaacgctgg taaaagatat
    37981 tatcaccggg gtatttattc agtttgaaaa cggcatgaac actggagatt tggtgactat
    38041 cgggccgttg accggcacag tggaacggat gtcgattcgc tccgtgggcg tgcgccagga
    38101 taccggggcg tatcacatca ttccgtggtc ttcgataacc acctttgcta acttcgtccg
    38161 cggtattggt tcggtagtgg caaactacga tgttgatcgc catgaagatg ctgataaagc
    38221 caatcaggca ctgaaagatg cggtaacgga attgatggaa aacgaagaaa ttcgcgggct
    38281 gattattggt gaaccgaact ttgccgggat tgtcggctta agcaataccg cgtttacact
    38341 gcgtgtttcg ttcaccacgc tgccactcaa acagtggacg gtacgctttg ccctcgacag
    38401 ccaggtgaaa aaacatttcg acctggcggg cgttcgcgcg ccagtgcaga cttatcaggt
    38461 gctgcctgct ccgggcgcga ccccggctga accgctgccg ccgggggagc caacgcttta
    38521 acgttggcga ttaacaaaac ggcggcgctg ctcgtcgttc ataaaggtcc aggcaataaa
    38581 gcgactctgc ttttgcccct gggccatctc tttttttacc accttcaccg cgcccacgtc
    38641 ggtcagagca cgatacaacg gcggtaagtt ttcaccacga gataccagcg aggtaaacca
    38701 catcacctgc ttcgcgaagc ctttgctctc ttcaatcatc tttttgataa agttgacttc
    38761 accgccttca caccacaact cctgttgctg accaccaaag ttcagtgcat cgtctttgtt
    38821 cagccccagg ttacggcgtt tacgctcact acctgcccgc gccgcagcgg cggaatcgtg
    38881 gaatggcggg ttacacaagg tcgcgtcgta ttgctcgttt ttgtggatga tgccgttaaa
    38941 aatcgcccca ctctcttttt gccgacgcag acgaatggcg cggttaagcc ccggattagc
    39001 actgataatc gcctgcgcac tgcttaacgc ctggctgctg gtttcactac cggtaaaacg
    39061 ccagccatat tcatgtacgc caatcagcgg ataaatacag ttcgcaccaa cgccgatatc
    39121 cagaatgctg gcattcgctg gaattgttcc gcttgcttca gccagtaaat cggcaaggtg
    39181 atgaatataa tccgcccggc ccggtactgg tgggcagaga aaaccgtcgg ggatatccca
    39241 gttcgctacg gcgtaaaaat gggccagcaa cgccttattg agcgccttca ccgccagcgg
    39301 attggcaaag tctacgcttt gctcccctgc gggtgtaagc gtgaggaatt gcctgagttc
    39361 aggattgacc tgacaaagcg tggcgagatc gtagcggctg tgatgacggt tgcgcggatg
    39421 caaccccggt ttctgggcgg acatagcatt ctcctttaag cagcggcgta agatacccgc
    39481 ataacgcgac agggtaaata tctggctgcg tttatcattc cccaaaacct taacatggct
    39541 atggtttatg gcaattcatc ataaaaatca gcattatgca tgtcattgat ttgtcttatt
    39601 ttgtaaaaat atatcgttca aaaaataagc atttctctgt caattttttg cgtcatttgc
    39661 atcgcgcagg cgacctacac ttatcaggca ctacccgttg tatttatgag gatgtcatta
    39721 tgaaaaagtg cctcacacta ctgattgcca ccgtcctgag cggaatctct ctcacggctt
    39781 atgccgcgca accgatgagt aacctggaca gcggtcagct acggcccgcc ggtaccgttt
    39841 cggcaactgg cgcatcaaac ctaagcgatc tggaggataa actggcagaa aaagcgcgcg
    39901 aacaaggcgc gaaaggttat gtcattaatt ccgcaggcgg aaatgaccag atgttaggta
    39961 ctgcaaccat ctacaaataa ccccaccaac gtaactgcat tcgcctctgg atgcagtttt
    40021 cgcctgtttt gtaatcaaaa aacatcaaat gtgaacgcaa tgtaaataaa tgtatttctt
    40081 tttcgcgcaa tgggtgatag aaaatcgcat caagtgataa tgcttatcaa aattattatc
    40141 acttgcacga acactatcac gggattaaca gtggcatcgc atccgcagag atgctttctc
    40201 gtggcagtga aaatttcaac atataagaaa aagtcacctg caaaatggaa aacaatcgca
    40261 atttccctgc cagacaattt cattcgctca cgttctttgc cggtctttgt attggtatta
    40321 cgcctgtggc acaggcactc gccgccgaag ggcaagctaa cgcggatgac acgctggttg
    40381 tcgaagcatc aacgccttcg ctttatgcgc cacaacaatc tgccgatccg aaattctcgc
    40441 gtccggtagc ggatactacc cgcacgatga cggtgatttc tgaacaagtg attaaagatc
    40501 agggcgcaac caaccttacc gacgcgctca aaaac
//
LOCUS       DAJTRD010000028        38849 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTRD010000028 DAJTRD010000000
VERSION     DAJTRD010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38849)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38849)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38849
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.028"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..155
                     /locus_tag="OO315_001629"
     CDS             <1..155
                     /locus_tag="OO315_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5890581.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="OO315_001630"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="OO315_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5890582.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="OO315_001631"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="OO315_001631"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5890583.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLSIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="OO315_001632"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="OO315_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="OO315_001633"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="OO315_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5890584.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="OO315_001634"
     CDS             complement(5626..7134)
                     /locus_tag="OO315_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5890585.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO315_001635"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO315_001635"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5890586.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="OO315_001636"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="OO315_001636"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5890587.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="OO315_001637"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="OO315_001637"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5890588.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO315_001638"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO315_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5890589.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO315_001639"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO315_001639"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5890590.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="OO315_001640"
     CDS             complement(14283..15602)
                     /locus_tag="OO315_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5890591.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO315_001641"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO315_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5890592.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="OO315_001642"
     CDS             16699..17034
                     /locus_tag="OO315_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5890593.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO315_001643"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO315_001643"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5890594.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="OO315_001644"
     CDS             complement(17790..19172)
                     /locus_tag="OO315_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5890595.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="OO315_001645"
     CDS             complement(19358..20302)
                     /locus_tag="OO315_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5890596.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="OO315_001646"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="OO315_001646"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5890597.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="OO315_001647"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="OO315_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5890598.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO315_001648"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO315_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5890599.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO315_001649"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO315_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5890600.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="OO315_001650"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="OO315_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5890601.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="OO315_001651"
     CDS             complement(25947..26768)
                     /locus_tag="OO315_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5890602.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="OO315_001652"
     CDS             complement(26868..26951)
                     /locus_tag="OO315_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890603.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="OO315_001653"
     CDS             complement(27044..27379)
                     /locus_tag="OO315_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5890604.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27776..29029)
                     /locus_tag="OO315_001654"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="OO315_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30065
                     /locus_tag="OO315_001655"
     CDS             29136..30065
                     /locus_tag="OO315_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5890605.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAELAELAKEPFVFFDPHVGTGLY
                     DDILGLMRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAE
                     EDAVSEMWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30200..31420
                     /gene="mlc"
                     /locus_tag="OO315_001656"
                     /gene_synonym="dgsA"
     CDS             30200..31420
                     /gene="mlc"
                     /locus_tag="OO315_001656"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5890606.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31545..32240
                     /gene="bioD"
                     /locus_tag="OO315_001657"
     CDS             31545..32240
                     /gene="bioD"
                     /locus_tag="OO315_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5890607.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32193..33485)
                     /gene="clcB"
                     /locus_tag="OO315_001658"
     CDS             complement(32193..33485)
                     /gene="clcB"
                     /locus_tag="OO315_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5890608.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISQTLHRDSIYRQHTAQHS"
     gene            complement(33644..34258)
                     /gene="dmsD"
                     /locus_tag="OO315_001659"
     CDS             complement(33644..34258)
                     /gene="dmsD"
                     /locus_tag="OO315_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5890609.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34301..35155)
                     /locus_tag="OO315_001660"
     CDS             complement(34301..35155)
                     /locus_tag="OO315_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5890610.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35157..35774)
                     /gene="dmsB"
                     /locus_tag="OO315_001661"
     CDS             complement(35157..35774)
                     /gene="dmsB"
                     /locus_tag="OO315_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5890611.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35785..37823)
                     /locus_tag="OO315_001662"
                     /pseudo
     CDS             complement(35785..37823)
                     /locus_tag="OO315_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37884..>38849)
                     /locus_tag="OO315_001663"
     CDS             complement(37884..>38849)
                     /locus_tag="OO315_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5890612.1"
                     /translation="EILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPN
                     DYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLY
                     AKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSS
                     KLAEIARTWELEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNI
                     DVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQG
                     AWHEPNMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
BASE COUNT         9872 a         9412 c         9951 g         9614 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaagcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgcgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagagt acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgtgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gctgaactgg ctgaactggc gaaagaaccc tttgtctttt
    29761 ttgatccgca cgtcgggact gggctgtatg acgatattct cggactgatg cgacgttacc
    29821 atttgacgcc cgtcatcact caggaggtgg gcgaggcaat gaccatcatc ggtctggttt
    29881 ccgccggtct gggtgtttca attttgcctg cgtcatttaa acgtgttcag ctcaacgaaa
    29941 tgcgctgggt gccgattgct gaagaggatg cggtttctga aatgtggttg gtctggccga
    30001 aacatcatga acaaagtcca gctgcgcgta actttcgtat tcatctgctg aatgctctca
    30061 ggtgagggaa atttcagcga aaaagcccga aaaatgtgct gttaatcaca tgcctaagta
    30121 aaaatttgac gacacgtatt gaagtgcttc accatagcct acagattatt tcggagcgcg
    30181 aaaatatagg gagtatgcgg tggttgctga aaaccagcct gggcacattg atcaaataaa
    30241 gcagaccaac gcgggcgcgg tttatcgcct gattgatcag cttggtccag tctcgcgtat
    30301 cgatctttcc cgtctggcgc agctggctcc tgccagtatc actaaaattg tccgtgagat
    30361 gctcgaagca cacctggtgc gagagctgga aatcaaagaa gcggggaacc gtggccgtcc
    30421 ggcggtgggg ctggtggttg aaactgaagc ctggcactat ctttctctgc gcattagtcg
    30481 cggggagatt ttccttgctc tgcgcgatct gagcagcaaa ctggtggtgg aagagtcgca
    30541 ggaactggcg ttaaaagatg acttgccatt gctggatcgt atcatttccc atatcgatca
    30601 gttttttatc cgccaccaga aaaaacttga gcgtctaact tcgattgcca taaccctgcc
    30661 gggaattatt gatacggaaa atggtattgt acatcgcatg ccgttctacg aggatgtaaa
    30721 agagatgccg ctcggcgagg cgctggagca gcataccggc gtaccggttt atattcagca
    30781 tgatatcagc gcatggacga tggcagaggc cttgtttggt gcctcacgcg gggcgcgcga
    30841 tgtgattcag gtggttatcg atcacaacgt gggggtgggc gtcattaccg atggtcatct
    30901 gctacacgca ggcagcagta gtctcgtgga aataggccac acacaggtcg acccatatgg
    30961 taaacgctgt tattgcggga accacggctg cctcgaaacc atcgccagtg tggacagtat
    31021 tcttgagctg gcacagctgc gtctcaatca atccatgagc tcgatgttac atgggcaacc
    31081 gttaaccgtg gactcattgt gtcaggcggc attgcgcggc gatctactgg caaaagacat
    31141 cattatcggg gtgggcgcgc atgtagggcg cattcttgcc atcatggtga atttatttaa
    31201 cccacaaaaa atactgattg gctcaccgtt aagtaaagcg gcagatatcc tcttcccggt
    31261 catctcagac agcatccgtc agcaggccct tcctgcgtat agtcagcaca ttagcgttga
    31321 gagtactcag ttttctaacc agggcacgat ggcaggcgct gcactggtaa aagacgcgat
    31381 gtataacggt tctttgttga ttcgtctgtt gcagggttaa cattttttaa ctgttctacc
    31441 aaaatttgcg ctatctcaat ttgggccagg aaagcataac ttagactttc aaggttaatt
    31501 attttcctag tttatatttg tgaagcataa cggtggagtt agtgatgctg aagcgtttct
    31561 ttattaccgg tacagacact tctgtaggga aaacggtggt ttcccgcgca ttgctacaag
    31621 cgttagcctc ccagggaaaa agggttgcgg gatataaacc cgtagcgaag gggagcaaag
    31681 agacacccga agggctgcgt aataaagatg ccttggtgtt gcagagtgtt tcaaccatcg
    31741 aactgcctta tgaagcagtt aatcctatcg cgttaagcga agaagaaagt agcgtggcgc
    31801 acagttgccc aatcaattac accctcattt caaacggcct ggcaaacctg accgataaag
    31861 tcgatcatgt cgtggtagaa gggactggcg gctggcgcag tctgatgaat gatttgcgtc
    31921 cactctctga atgggtagtg caggaacaac tgccggtgtt gatggttgtc ggtattcagg
    31981 aaggttgcat taaccatgca ctgctaacag ctcaggcgat cgccaacgac gggctgccgc
    32041 tcattggctg ggtggctaac cgaatcaacc caggactggc gcattatgcg gaaatcattg
    32101 atgtgctggg taaaaaactt ccggcaccgc tcattggtga actgccttat ctgccgcgcg
    32161 ccgaacagcg tgaactgggg caatacatcc gcttagctat gctgcgcagt gtgctggcgg
    32221 tagatagagt cacggtgtaa cgtctgcgaa attaccgacg caattacgca ggcaatcaat
    32281 aaaccgggga gtagctgata ctccccggtc atttcacata tcatcaacgt cgacataatc
    32341 ggcgcgtgcg tggttgccgc caacagtgtc gccatcccgg tcaatccgag taaaagcgta
    32401 atttcttcgc catcagggaa ccataatccc aggctacgac catacaacat gccaatggca
    32461 agaccgataa atagcgtcgg tgtaaagaca ccaccgggtg cgccggaacc gctactcgcc
    32521 agcacggcac acagtttaca aaggaagatc ccggcaatga tcattaacag tggtggggcg
    32581 gttaaaaagg attgtacggt gctatagccg ttgccccaca ctgcaggtgt gaacagggaa
    32641 agcagaccca cgatcaaccc gcctagtgcc agttgccagg gcggcgcaag tttgagactc
    32701 acaaatccac gatgacaggc gttcattaac gttaacaaca gtggtccgca cagacctgcc
    32761 agcacacctg tactgataat cagcgcatag tcacgagcct gaaccgtcac tgagagttgt
    32821 acgttgtaga gtaacgcgtc gctatgatta atcagattgc taaccagcca tgccacgatg
    32881 gcggaaataa tcaccgggcc gagagaggcc aacatcatag tgccaaacag cacttcggct
    32941 ataaataaac tgccagcaag cggggcacga taggccgcag ccattcccgc cgcggcccca
    33001 caggcgatcc ataatttcca ttcctggcgt ggcgtaaaac gttgggcaaa acaggaggcg
    33061 gcaagggcag ctaaaagaat catcgcacct tcgcgaccaa ttgcactgcc gctggttact
    33121 accagcagcg aggcaagcga tttaaccagg cttgctgcgt aatcgaactg tccatcggtt
    33181 tgcaacgctt ccatgtaatc ggtcggcgca tgagggcgtt gttgggtaaa tttctgccag
    33241 cccatcagca acaaacccgc cgccagtccg ccgagcgccg gagttagcag ccgtcgccag
    33301 gaggaaaggt ttgtcgctgc attgaccaga ctgccggagt cattattgag gaacaaccac
    33361 tccagtagca gcatcgcatg acgaaacccg gcaacggcaa agacagcgag aataccgacg
    33421 actgttgcga taagcagacg gcggaacata gtgcgcagat ccggatagat gtgtagatga
    33481 tgcatgagca gaaatgggca atgaaacacg ggcgactatt ttgcggcaaa agaggccaac
    33541 cgcaaaagca gtgcgtgctt taagagataa aaaaacgtga cattgtaacg tattattgcc
    33601 gaatcgcgcg taatcgtcac catccggcaa tattacggtg atcctatcga aagagtggtt
    33661 taaccgcgac aggaatcaac agttgtgatt gccattgcgc cagcgttaag cgagccagtt
    33721 cacccagtgc acggtagaaa gggtgttctg ctttttcgat aaaaacatca agaaaacgtg
    33781 ttgaccacgg aaaaaggtgc catgccagca attcttcgca ttctgtctgg cgaccattct
    33841 ccgccaacca cgcagccatc aacagcagtg aaccaaaatg atcttccggt tcgttttgct
    33901 tcatttcaaa ctgaatgcct ttctcgcgca tccactgacg aagtgccaat gttgaatcgc
    33961 caaacagcac agattcgcga tccagccaga ccgaccccca cggcggagac ggcagtgccc
    34021 acgggccgac aaacaaacgc tgccaggcct gggcgtgagt ctcttcacac tgtgtctgaa
    34081 aagccgtcac cagcggcgct aatgacgctt ctggtagagg ccactgagtt tcccagccgt
    34141 cactggtgag taccgcaacc agaggtgcgg cttccgcact ctctggagcg taataaaaca
    34201 gcgcgcccaa cacgcgcgcc gcgacagaaa aattatcttg ctgtgaaaaa tgggtcattc
    34261 ctaacatcct gaaatgtgcg ggttgccccg caccaatttg ttaacctgca atagccattc
    34321 ctactgtcat gtgcaggcca taaaagagca cacgcccgat catctcacca acaagtatga
    34381 ggattagccc cagaacaagt ccggcaacgt gaggttcacg gcgacgaatg agcggacaaa
    34441 gccagcatcc aagacctgcg cataacaata ccacacgcca gacctgtaag gaggcgtaat
    34501 ctggtaccag tgcgctggct tgttgcacgg atgagtgaat agacgccaga gacaaacctt
    34561 gcagcacaat cacctcggca caagcgatca gcgccagaac gctaatgatg gcaaatggtg
    34621 tggtattaaa tgtaacgcgg gctgcccgca gaattgcggc agcaagtatt ggaccgctca
    34681 acaataccgt caggaagaaa gccagcgtag tgtaaccgtt atgccaggtt ggcacggtgt
    34741 cgatttgata cacgcaggtc atcatccaga cgaaaatgac gccgagcgcc atgctgacaa
    34801 gtaaccagag tttccccaac gcttgcggca ttttaccgat gaccgccacc agccaccata
    34861 gtccgccaac ggcaaaaaat atggaaccgg cagcaatctc gttactcaga ccagaagctc
    34921 cgattcgatt cagcgagtta aacgctcgca gtggcgatcc caggtgcatg acagaggcaa
    34981 taaacccgat gcccatcaac agccagagga aaaacatgcc acgaacaatg tgctgacggt
    35041 ctgcgtcatt tttggcggcg aaccagccaa ttccgctaac aatcagcgcg cctactacgc
    35101 attgacccaa tacggtaaag ataaccagtg gccattcatg ccatccattt cccattttac
    35161 acttcctccg gattagccag ataaccagtg gtatcgccgg tcgggcggct gttggcgtta
    35221 ggtttgataa cgatattggg ttttgtgaaa tgcgcgcgcg gcagtggcgc gacggcagcc
    35281 agagtgccgt gcttctggcg cagctcttca attggaccga actcaagtgc acgcagcggg
    35341 caggattcga cgcatatggg ttgtttcccc tcggcgacgc gcgaataaca accatcgcac
    35401 ttcgtcatgt gccctttttc agcattgtac tgtggtgcgc cgtacggaca agccatgtgg
    35461 cagtagcggc agccgataca aacatcttca tcaaccacca caaagccatc ttcacgctta
    35521 tgcattgcgc cgctggggca gacttttgta catgcggggt catcgcaatg gttacaggag
    35581 atggagagat aataagcgaa cacgttctgg tgccagatgc cattatcttc ctgccagtcg
    35641 ccgccagcat attcataaat acggcggaaa ctgacttccg ggccaagatc tttaaaatct
    35701 ttgcacgcca gttcgcaggt tttacagcca gtacagcggc tggaatcgat aaaaaatcca
    35761 tattgtgtgg tcatgggcta ctccttaaac cttttcgatc tggacaagat tgctgtgcga
    35821 cgggtttccc tttgccagcg gtgaagggcg gtgagaggtc agaatattga tactgccgcc
    35881 gtgatcgacc cggtcaccaa acatatccgc tttaagccac gcaccttgcc cgatggcggt
    35941 aacgccaggc agaatacgcg gagtcacttt tgcggcaatc agcatttctc cattattgtt
    36001 aaatacccgc acggtatcgc catgacggat accgcgtgcc tgagcatcaa tggggttgat
    36061 ccacacctct tgtgggcagg cctgctgtaa cacatcaata ttgccgtagc tggagtgggt
    36121 acgcgctttg gtttggcgac ttcactcagc atccagtaga tgggtttgcg ttcaaatttt
    36181 gctgaggttg cgggttgggc gaggatcaca tagcccatat tccctgcaga ttcatgagag
    36241 ataaggtctt cttgctctgt tggcatcagg tcaggcaaca ggatatcgca atacttagcc
    36301 gaggccgtca tgaagtggtc aatgccaaca atcatctcgc acttgctgtc atcctgaagc
    36361 acctcatggg tgtgattgat gtcgccatgt tgattgatca atgtgttact ggcgtagcac
    36421 cataaaaact tgatggggac atccagtttt tcttttccac gaacaccatc acgggtcgcg
    36481 gtcattttcg taccatggtc gatggcatct gtccatgtaa aaacggaaat ctgcgtttta
    36541 acaggattct cgagcatcgg gaaccattct acccccagat cccagctacc ttcgcgtaca
    36601 cctgagttgc cgccgtttat gccgacgtta ccggtaagaa cggaaagcat ggcaatagcg
    36661 cgggacgttt gttcgccgtt ggagtgtcgt tgtggccccc aaccctgaca aatataagca
    36721 ggttttgctg aaccgatctc tcgtgccaac tggataattt tttctgccgg gatgctggtg
    36781 atttttgctg cccattccgg cgttttagct atgccgtcag gcccttcgcc cagaatatag
    36841 gctttataat gcgcgttacg tggtgcgttg gcgggcagcg ttttttcatc gtaaccaaca
    36901 caatatttgt cgagaaatgg ctgatcgacc atgttttcag taatcagtac ccaggcaatc
    36961 gcacaggcca gtgcgccatc ggtgccaggg cgaatgggca gccattcatc ttcacgcccg
    37021 gcagctgtgt cgttatagcg tggatcgatg acgatcatgc gtgcgtttga acgttcgcgg
    37081 gcttgctcga cgtagtaagt gacaccaccg ccgctcatcc gcgtttctgc cgggttattt
    37141 ccgaacataa cgaccagttt cgtattggcg atatcatccg ggctgttgcc atcattggca
    37201 ccgaacatat aactcattgc ggcactgatc tgtgcggtac tgtagctgcc atagcgactg
    37261 agaaaacccc cgcaagagtt catcagacgg tacgggacgt tcgagttggt gatatttccg
    37321 ccatctacgc ctgttccgta caggacatgt acagcctcat tgccgtaatc tttcaggatc
    37381 cgccgaagat tatcactgat ggtatccagg gcttcgtccc aacttatccg ttcaaattta
    37441 ccttcaccgc gctcgccgac gcgcttcatg ggatatttca acctgtcagg atgattcatc
    37501 cgtcggcgga tagagcgccc gcgtaaacat gcccgaacct gatgattacc gtaaacgtcg
    37561 tcacctgtcg tatcagactc cacccagtac acggtgtcat ctttcacatg caaacgtagc
    37621 agacagcggc tcccgcagtt aacggtgcag gaactccaga ccgttttctc ttctaccgga
    37681 gcctctgccg cccggaccat ttgggaaaat ggcagagtga aagcactgct tgccagcgcg
    37741 agactgccaa gtgcggaggt tttcatcaga cttctatggc tgatttcagc cttcatgagc
    37801 gcctctgtgg tatggatttt catcattact cacttattgc ttttcaaaca aaatgtcatg
    37861 ccagaattta tggttgtagt gggttatatt ttttcgatct cgaccagatt agtgtgctgc
    37921 gggtttccct tcgccagtgg tgaagggcgc agagtggtta gcgtattcac acatccgcca
    37981 tggtcgattt tatcgccaga catattgggc tcgtgccagg ctccctggcc catagcgcta
    38041 actccaggga gaatgcgtgg tgttactttg gctggtagcc gaacttcgcc acgatggtta
    38101 aacacccgca ccatatcgcc gttggcaatc ccacgtttct gtgcatctat agggttgatc
    38161 cacacctcct gacggcaggc tgccttcagg acatcaatgt tgccgtaggt cgagtgagta
    38221 cgggatttgt aatggaaacc aaacagttgc agtgggaagg ttctacgttc aggggagtcc
    38281 cagccttcaa aggttgaggc ataaactggc agtgggctta tcacttcatc tttttccagt
    38341 tcccaggtac gggcaatttc cgccagcttg ctggaataaa tttcaatctt accggaaggc
    38401 gttttaagtg gatttgcctc ggggtcgtca cgaaatgctt tgtaggcgac aaaatggcca
    38461 ttgggatctt tacgcttata gatacccatt tttttcagtt cgtcgtaaga cggtaacgcc
    38521 ggatctttgg caagcatttt ggcgtacaga tgttgtaacc attgttcctg cgtgcgacct
    38581 tctgtgaact tttgatagac gtcaggtcca agacgtttcg cgacttcact caggatccag
    38641 taaatcggtt tacgttcgaa tttttcgctg gtgactggct ggaggaaaat gagatatccc
    38701 atgttaccgg cgtagtcgtt aggaataata tcttcctgct caacggtcat caggtcttcc
    38761 agcagaatgt cggcatattt tgccgatgag gtcataaagt tttcgatgac cacaatcatt
    38821 tcgcatttcg attcgtcctg cagaatttc
//
LOCUS       DAJTRD010000029        37628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTRD010000029 DAJTRD010000000
VERSION     DAJTRD010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.029"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..176)
                     /locus_tag="OO315_001664"
     CDS             complement(<1..176)
                     /locus_tag="OO315_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5890613.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
     gene            complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OO315_001665"
     CDS             complement(260..1144)
                     /gene="yhdJ"
                     /locus_tag="OO315_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5890614.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OO315_001666"
     CDS             complement(1230..1526)
                     /gene="fis"
                     /locus_tag="OO315_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5890615.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OO315_001667"
     CDS             complement(1552..2517)
                     /gene="dusB"
                     /locus_tag="OO315_001667"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5890616.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OO315_001668"
     CDS             complement(2846..3727)
                     /gene="prmA"
                     /locus_tag="OO315_001668"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5890617.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OO315_001669"
     CDS             complement(3739..5190)
                     /gene="panF"
                     /locus_tag="OO315_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5890618.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(5180..5422)
                     /locus_tag="OO315_001670"
     CDS             complement(5180..5422)
                     /locus_tag="OO315_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5890619.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OO315_001671"
     CDS             complement(5531..6880)
                     /gene="accC"
                     /locus_tag="OO315_001671"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5890620.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OO315_001672"
                     /gene_synonym="fabE"
     CDS             complement(6891..7361)
                     /gene="accB"
                     /locus_tag="OO315_001672"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5890621.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(8339..9313)
                     /locus_tag="OO315_001673"
     CDS             complement(8339..9313)
                     /locus_tag="OO315_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5890622.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            9465..11405
                     /gene="csrD"
                     /locus_tag="OO315_001674"
     CDS             9465..11405
                     /gene="csrD"
                     /locus_tag="OO315_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5890623.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11710..12753
                     /gene="mreB"
                     /locus_tag="OO315_001675"
     CDS             11710..12753
                     /gene="mreB"
                     /locus_tag="OO315_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5890624.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12819..13922
                     /gene="mreC"
                     /locus_tag="OO315_001676"
     CDS             12819..13922
                     /gene="mreC"
                     /locus_tag="OO315_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5890625.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13922..14410
                     /gene="mreD"
                     /locus_tag="OO315_001677"
     CDS             13922..14410
                     /gene="mreD"
                     /locus_tag="OO315_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5890626.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            14419..15012
                     /gene="yhdE"
                     /locus_tag="OO315_001678"
     CDS             14419..15012
                     /gene="yhdE"
                     /locus_tag="OO315_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5890627.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            15002..16471
                     /gene="rng"
                     /locus_tag="OO315_001679"
     CDS             15002..16471
                     /gene="rng"
                     /locus_tag="OO315_001679"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5890628.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16539..20339
                     /gene="yhdP"
                     /locus_tag="OO315_001680"
     CDS             16539..20339
                     /gene="yhdP"
                     /locus_tag="OO315_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5890629.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            20495..21940
                     /gene="tldD"
                     /locus_tag="OO315_001681"
     CDS             20495..21940
                     /gene="tldD"
                     /locus_tag="OO315_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5890630.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OO315_001682"
     CDS             complement(22068..22997)
                     /gene="aaeR"
                     /locus_tag="OO315_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5890631.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            23180..23383
                     /locus_tag="OO315_001683"
     CDS             23180..23383
                     /locus_tag="OO315_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5890632.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            23391..24323
                     /gene="aaeA"
                     /locus_tag="OO315_001684"
     CDS             23391..24323
                     /gene="aaeA"
                     /locus_tag="OO315_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5890633.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            24329..26296
                     /gene="aaeB"
                     /locus_tag="OO315_001685"
     CDS             24329..26296
                     /gene="aaeB"
                     /locus_tag="OO315_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5890634.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            26388..26660
                     /locus_tag="OO315_001686"
     CDS             26388..26660
                     /locus_tag="OO315_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5890635.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OO315_001687"
     CDS             complement(26716..26979)
                     /gene="yhcN"
                     /locus_tag="OO315_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5890636.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OO315_001688"
     CDS             complement(27344..27814)
                     /gene="argR"
                     /locus_tag="OO315_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5890637.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            28249..29187
                     /gene="mdh"
                     /locus_tag="OO315_001689"
     CDS             28249..29187
                     /gene="mdh"
                     /locus_tag="OO315_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5890638.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OO315_001690"
     CDS             complement(29249..30316)
                     /gene="degS"
                     /locus_tag="OO315_001690"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5890639.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OO315_001691"
                     /pseudo
     CDS             complement(30406..31753)
                     /gene="degQ"
                     /locus_tag="OO315_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31907..32305)
                     /locus_tag="OO315_001692"
     CDS             complement(31907..32305)
                     /locus_tag="OO315_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5890640.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            32499..33626
                     /gene="zapE"
                     /locus_tag="OO315_001693"
     CDS             32499..33626
                     /gene="zapE"
                     /locus_tag="OO315_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5890641.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33845..34273
                     /gene="rplM"
                     /locus_tag="OO315_001694"
     CDS             33845..34273
                     /gene="rplM"
                     /locus_tag="OO315_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5890642.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            34289..34681
                     /gene="rpsI"
                     /locus_tag="OO315_001695"
     CDS             34289..34681
                     /gene="rpsI"
                     /locus_tag="OO315_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5890643.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            35076..35714
                     /gene="sspA"
                     /locus_tag="OO315_001696"
     CDS             35076..35714
                     /gene="sspA"
                     /locus_tag="OO315_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5890644.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35720..36217
                     /gene="sspB"
                     /locus_tag="OO315_001697"
     CDS             35720..36217
                     /gene="sspB"
                     /locus_tag="OO315_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5890645.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="OO315_001698"
     CDS             complement(36260..37627)
                     /gene="dcuC"
                     /locus_tag="OO315_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5890646.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASCAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMPFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
BASE COUNT         9115 a         9414 c         9901 g         9198 t
ORIGIN      
        1 attctgtcct gaaaattcag ttcgtcacag ttaaccagtt ttagtggatt aactgagttc
       61 atattacggc atttgtctgt ttctgttgtc agcagttcaa tccactgcat cagtaaagta
      121 tcaaacagaa aaacgaagac agcgaaaacg acgagccacc aatacttgcg aatcattacc
      181 tgagcctgtg catgtttaac caagtctggc aggaaatata cacgaaatga ataatctgaa
      241 aactaggctt acacttcctt tactttgcaa tgagatcggg gtcaacttcg cttaaccggc
      301 ttcgttttga caggttgccg gtctttcttt ttttcacttt cgccagttct tccgcggagt
      361 aatgcgacgc gacatccagc cgtcgaagcc ccattttgat gtactcgctg ttgatctcaa
      421 taccaatgaa ttttcgtccg ctggcgatgg ctacggcacc ggtagtaaag ctaccggcaa
      481 acgggtcgag aacgatatcg cctgggttgg aagaggcgag aataatgcgt ttcagtaagg
      541 ctttcggttt ttgcgtcggg tggttctcat attcatccat caaataacgc acgcgcggaa
      601 aatcccagac gttacccggt actttttgat gattgtatgg ctgtggagga tttttgcgat
      661 aatcgatcaa cgcgcgctgc gatccggttt tggcttctac cagaatagca tcaccgttga
      721 atgtgtagtt ctttgcgtct ttcaccatca tcaggatggg ttcgtacatg gagccgtagt
      781 gttttttcgc ctgtactcca gaactgtcat atgaccagac gatgcgactt ttgatggtaa
      841 aaagcttgcg gcactggaga tcgataaagg gcatgttttc cgtactgttc ataatgtaca
      901 tgctgccctg ctttttcaga acgcggtggc actctgcaat cacttcaaac agccagtcga
      961 taaacagatc ttctttccag gcttcgatca gaccatcaaa atttttaccg atgttatacg
     1021 gtgggtcggc aaagagcaga tcgacgcttt cagtaggtag ctttttaagt tcggcaaagg
     1081 catcaccgtg aataatggtc ttagcttcat taccaaaccg ggtcggttca catcctgttc
     1141 tcatggtcac tccctttgtg acacctataa aaaaggcgct tccccatgcc gagtagcgcc
     1201 tttttaatca agcatttagc taacctgaat tagttcatgc cgtatttttt caatttttta
     1261 cgcagcgtac cacggttgat gcccatcatc agcgcagcac gggtctggtt accacgggtg
     1321 tattgcatca ccatgtccaa caggggctgt tctacttcag ccagtaccag ctcatagagg
     1381 tcattcacat cctgaccatt cagttgagca aaatagttct tcagtgcctg tttaaccgag
     1441 tcacgcaggg gtttttgggt tacctgatcc tgagagttaa cggtagaaac ggtcagtacg
     1501 tcagaattta cgcgttgttc gaacatagtt ctgtcagctc tttatttctg tttacgcaaa
     1561 attttcgaag tatgcctcca acgcctccag ctgttcgctg gcatcctcaa tggcgttgaa
     1621 tgtgcgccga aactggtcat ttggagcgtg ttcctggaga taccaggaaa cgtgtttacg
     1681 tgcaattcgg tacccttttg ccggaccata aaagtcatgc agttcccgaa cgtgcgcgca
     1741 aagcaagcgc ttaacctctg ccaaaggcag tgggggcagc aactccccag tgtccagata
     1801 atgctggatt tcccgaaaga tccagggtct tccctgagct gcgcggccta tcatcagggc
     1861 atccgcccct gtatagtcga gcacagctct ggctttaagc gggtcagtaa tgtcgccatt
     1921 cgcgataacc ggaatggaaa ctttctgctt aactgcccga atactgtcgt actcagcttc
     1981 tccattgaac aaacaggcgc gtgtacggcc atgaatggtc agagcctgaa tgccacagtc
     2041 ttcagccagt tgggcaatct cttcgcagtt acggtgttcc ggtgcccagc cggtgcgaat
     2101 cttcagggta acaggaacgt ccactgcatt gacgacctcg gtaaggatcg atttaacgac
     2161 atccgggtac tgcaagaggg ctgaacctgc gagcttgcga ttcacttttt tagccgggca
     2221 acccatattg atatcaataa tctgggcacc gctttccacg ttaatacgtg ctgcatctgc
     2281 catttctttc ggagcgctac cagcaatttg cacggtgcga ataccgggtt catcaatgtg
     2341 caccatccgt aaacgagatt tgtcgctttc ccaaacctgt gggttagaag acatcatctc
     2401 ggatactgtc aatccggctc ccatctcgta gcacaacgtc cgaaaaggtc tgtctgtaat
     2461 gccagccatg ggcgctgcga tcaggcgatt tctgagctga tattgtccga tgcgcatgag
     2521 ttaagaaatg accatactgt gactgcaagg cggcgtatat tacgcatttt ttgcacgaga
     2581 tgaaaggcca aactttgacc aatcctctga gatggatcaa agaattgcat ttaaaatgag
     2641 cgtggtgcga taattactca taaaaatcat catattagaa aatagtgact aaaatttaca
     2701 ctcaaagaaa tttgagtaag ttctcaattt ttctttatga atgaaaattt tggcacgcaa
     2761 atttgcgtaa ataatcggca aatttacgtg ccttttgtga gcttgctcgc acttcgcccc
     2821 gcgtcaccct acggcgatgc gaaggtcaat tcttacgacc ggtaatacgg caccactctt
     2881 ctttttccac gaccgggtcc agtgcgaagc tatcggcata agcttcacaa acgctctctg
     2941 cctggcttgc cagaataccg gaaaggccca gcaaaccgcc tgaaactggc aggacgctga
     3001 ttaacggtgc cagttcacgt aatgggcctg caaggatgtt agcgaccacc acgtcggctt
     3061 tcatttcttc tggctgatct ttcggtaagt agagttccag acggtcagaa acgccattac
     3121 gttcggcgtt atcgcggctg gcctgaatcg cctgcggatc gatatcaata ccaatggctt
     3181 ttgctgcacc cagtttcagc gccgcgatcg ccagaatgcc ggaaccacag ccaaagtcga
     3241 tgactgtttt accggttaaa tcgaggctgt cgagccattg caggcacaga gaggtggttg
     3301 gatgggtacc cgtaccaaac gccagccctg gatctaacat cacgttgacg gcgttttcgt
     3361 ccggcacatc acgccagcta gggcagatcc acagtcgttc accaaagcgc atcgggtgga
     3421 aattatccat ccattcgcgc tcccagtctt tatcttctag ttgttcgatt ttatgcgcga
     3481 agcctgcgcc gagcagcgga tggttttcca gaatcgccac cacgtcgttc atatcggttt
     3541 cagcgtcgaa cagaccaatc acatcggtgt cgccccacag gcgcgtttcg cccggcagcg
     3601 gctcaaatac tggcgtatcg tgggtatcct gaaaagtgat agaaacggca cccgcttcca
     3661 tcagcgcatc gctaagatct tccgcgttcg cgccggtggt gttcagtttc agttggatcc
     3721 aaggcatggc aaaactcttt atttatcagt agtcaaaacg gtagcttgcg ggacggatgt
     3781 accgaaacgg tttccgacca ggaaagccag caaacttagt agtaacgagg gcacgatagg
     3841 gtggaagccc aggtactgaa tattcagcgt cgcgagtacg gcatacagca cgccgccaac
     3901 gatcatcgca cttagcgcgc ctttggcgtt ggcgcgttcc cagtaaagac ccagcaccag
     3961 cggccacagg aaaacggctt ccagcccacc gaaggccagc aaattcagcc agatgatcat
     4021 ttctggcggc ttccaggcgg caagcagcag caacgcgccg agaactaacg taattaccgc
     4081 cgacatccgc ttcagacgcg tctcgttttg catttgatcc ggacggatat tcagatagag
     4141 atctttaatg atcgtagcgg aactttgcag cagttgggcg ttaattgtcg acatgatcgc
     4201 agccataggt gcagccagaa agatcccggc agcaaaaggt ggcagcactt ttaccattaa
     4261 cgtcgggatc accagatctg gtacggtgag atcggggatc actgcacgac ctaacgctcc
     4321 ggccaggtgc ataccgaaca tcaggattgc gactataatc gtaccgatga tgatcccccg
     4381 gtgtactgct ttgctgtctt tataagagat acagcgcacc gcagtatgcg gcaggccaat
     4441 cacgccaaag cacaccagta cccagaacga cgtcataaag gcaggcgaca gaatatcgtc
     4501 agcgccttgt ggcgtaacca gttgcggatc gatggtttgc aaggtctgta ctgcgttgct
     4561 taagccgcca gcggcatgta ccacgccaat aagcagcaca acggtgccaa tcagcatcac
     4621 aagcccttgc atggtgtcgt tcagcacgct ggcgcgaaag ccaccaaagg cggtatataa
     4681 cgcaatgctg ataccaaaaa tcagcagccc ggtttcataa ggaatacccg ccgcggtttc
     4741 cagcaggcgc gcaccgccga taaactgcac ggtcatcgca ccaacgaacg caaccagcaa
     4801 actcaaactc gccagccaca ccagaagacg actctggtag cgggcaaaca gcatatcgtt
     4861 cagcgtcact gcattgtagc ggcgcgcaag aatcgcaaac ttcttgccga gaataccgag
     4921 tgaaagccag acagcaggaa gctgaatcat cgccagcaat acccagccca gcccgtattt
     4981 ataagcagct cctggcccgc cgataaacga actggcactg atataggtcg cggtgagagt
     5041 catcgccagc acaataccgc ccatagagcg gctgccgagg aaatactcat taaggaaggt
     5101 gccggtgctc cgtttacgca tcgcataaac cgagataccg aacaccacca ccagataggc
     5161 gaccagcggt agaattactt caagctgcat cgtcatcctc cagtgggata tcgcgataga
     5221 taaatttcac catcgcccag cacagtccaa taaacagtag cggcgtcagg atgcaggcca
     5281 tctcaaacca gcgcggaaag ccggtaaaac cgggggcaac gccaggtaag taagcgtcta
     5341 ctaaccaaac tgccagatac aaaagggtca gccccagcgc ccagcgggcc tctttatggg
     5401 cctgaacaaa acgagtgtcc attttttgtc cctgatgggt gaagaaagcg aggattgtac
     5461 cttatggggg ttgtcgatcc ccagtaataa aaaaggccgg aaaatccggc cttttgacgc
     5521 ttttgcagtc ttatttttcc tgaagaccga gttttttctc cagatagtgg atgttagtgc
     5581 caccatgctg gaagttctcg tcattcatga tgcggatctg cagatcaacg ttggttttga
     5641 taccgtcgat gatcagctcc tgcagcgcat tcttcatgcg ggcaatcgcc acgtcacggt
     5701 tttcaccgta gcaaatcagc ttaccgatca ttgagtcata gtacggcggt acggtgtagc
     5761 ccgcgtagat atgagactcc caacgtacgc caaaaccgcc aggtgcgtgg aaacgggtga
     5821 ttttgcctgg gcttggcagg aaggtgttcg gatcttcggc gttgatacga cattccaccg
     5881 catggccgcg aacgtgaact tcttcttgct tgatcgacag cggttgaccg gcagcgatac
     5941 gcagctgttc tttgatcagg tcaacgccgg tgatcatttc tgtaaccggg tgttctacct
     6001 gaatacgggt gttcatttcg atgaaataga actcgccgtt ttcgaacagg aactcgaaag
     6061 tacctgcacc gcgatagccg atatcaacac acgctttagc gcaacgttcg ccgatgtagc
     6121 gacgcagttc cggggtaatg cccggtgctg gtgcttcttc gaccactttc tggtggcggc
     6181 gctgcatgga gcagtcacgt tccgccagat agatagcgtt gccctgaccg tcagccagta
     6241 cctgaatctc gacgtggcga ggattctcca ggtatttctc catgtaaacc atatcgttgc
     6301 tgaaagcagc tttcgcttcc gcacgggtca tggagatgga ttgtgccagt tcagcgtcgc
     6361 cgcgcactac gcgcataccg cgaccgccgc cgccgccgga ggctttgata atcaccggat
     6421 aaccaatgcg tttagcaatg gcgcggtttt tatccatatc gtcgcccagc gggccgtcag
     6481 aaccaggtac gcaaggaacg cccgcttttt tcatcgccgc gattgcggat actttgtcgc
     6541 ccatcaggcg aatggtttct gctttcgggc cgatgaagat aaagccggag cgttcaacct
     6601 gctcggcaaa gttggcgttc tcggagagga agccgtaacc cggatggatt gctactgcgc
     6661 cggtgatttc agcggcgctg atgattgccg ggatgttcag ataacttttt actgacggag
     6721 cagggccaat acagaccgtt tcatctgcca gtaatacgtg ttttagatcg cgatccgtgc
     6781 tggagtgcac agcgacagtc ttgatgccca gttctttaca ggcacgaaga atacgcaatg
     6841 caatctcgcc gcggttggca ataacaattt tatccagcat gttcgcctcg ttactcgatg
     6901 acgaccagcg gctcgtcaaa ttctaccggt tgtccacttt cgaccagaat tgctttcacg
     6961 gtaccggatt tgtccgcttc gatctggttc atcattttca tggcttcaac gatgcacagg
     7021 gtatcgccca cgttgacttt ctgacccact tcgatgaacg cttttgcgtc cgggcttggg
     7081 gtgcggtaga aagtaccaac catcggggaa cgtacgatgt gaccactgat ttccgctgct
     7141 gctggcgctt ccatggaagg aacggtcgcc ggagcggctg cgttagattg agctggctgc
     7201 tgcatcattg gtgcagcgta agcttgttgc atcacaggga aacttgcggc aggagctgca
     7261 cggctaatgc gtactgactc ttcgccttca gaaatttcca gttcggagat gcctgattct
     7321 tcaaccagct cgatcagttt tttaatctta cgaatatcca tgagtgggtt ccgtactctt
     7381 tgtttagtgt gattgtgaca ggcgtttcac cgccgtctgt aaagcgtatg ccccgacagc
     7441 tgtatgcata gcgataaatt ccagcaggcc gggacgccgg tctattttgc cccagagtgt
     7501 caatgttaga cttgacggac attgtgcagc gatctgctgc tatcctccgg caaaaaacaa
     7561 aatatacctt cgttgcgcca atgtcacctt ttccgcagcg caaaaactgc gtcagggagg
     7621 acgaggtcgc acattataac gatttcgtag caattggcag ctaaatactg gtcttatcag
     7681 ggaatataat caacagctaa catgtaaata accttcaaca ccgtgtaatt tgcaacaagc
     7741 cgcacaattc acgaaattag cgccatcatc gacggaactt cttataacgt aaggtgaaaa
     7801 gctgaaaagc cagccctgcg aagatgaggg gctgcaatga gataatcttc acagaccaca
     7861 aataatgtag ggatgccagg atcgcgacaa gatagacgac attacgcagc ggttgctggg
     7921 gattacccaa ttttcactgc atcgtctgga tagatgtgaa tgctaaagca cgcaaaatca
     7981 accaactgac aattctcagc attaaatagg ttcgagtggt taatttgtag tcaaataaaa
     8041 tcagattatt caccccatgg taccagaaat cagtaacagg ttaagcgtag tgtcgcccag
     8101 gcaaagcacc ataaacctaa caggcgacgg aggcgtatca ataatggctg tttagaggag
     8161 tatcaatgcg catcaatttg tggtcagtcg aaatcatgac tttccagttt ggatgatgtt
     8221 gtacaacttc ctgaccaagg tgttcgacga aagctgtccg gctaaccgct tttcccgtta
     8281 accgattgaa taatgatgtc aaaatgagtt ccctctctta ttattccttg ctaaatggtt
     8341 agttaacctt caccagcgtg cgaccctgga tctggttatt aatgatggcc tcggcaaagt
     8401 tcggtgcctc tgacagagat atctcttttg ccgcctgggt atagaatgat tccggtaaat
     8461 cggcgaccag tcgctgccag gcttgtgcgc ggcgttctgg tggcgtcatt actgaatcca
     8521 ccccttgcaa acggacatta cgcagaataa atggcatgac cgtggttggc agagtaaaac
     8581 cacccgccag accacaggcc gccacgcagc cgccgtaatt catttgcgcc agcacttttg
     8641 ccagcacttt gtcgccaaca gtgtcaattg ccccagccca gacttgtttt tccagaggac
     8701 gggattcggc aaactcatca cgagggagaa taccgctggc acctaaactt ttcagatatt
     8761 catgggtact ttcgcgaccg gaaacggcaa cgacctgata acccaactta tgcagcagcg
     8821 ccacggcggt actgccgacg ccaccactgg cacccgtcac gacaatctca ccgtcctgcg
     8881 ggcgaacacc ggcatcttcc agcgccatca cacacagcat ggcggtaaaa ccggcagtac
     8941 cgataatcat tgctttacgc gcgtccagcc cttgcggcat ggcaaccagc cagtcacctt
     9001 tcactcgcgc ctgctccgcc agcccacccc agtggttttc accaacgccc cagccagtga
     9061 gtaacacctc ctgaccggca tgaaaacgcg gatcttcgct ggtgcgtaca gttccggcaa
     9121 aatcgatccc aggaatcatc ggaaaattac ggatgatttt tcccttaccg gtaatcgcca
     9181 gcgcatcttt atagttcagg ctcgaccagt gaacatcgac cgtgacatcg ccctccggca
     9241 ggcgacgttc gtccagagtc tgtactgatg cgagagtttt gccgtcctgc tgttctaaaa
     9301 gtaacgcctg cataagtggt cctcatgtgc atgatggatt ggaaaataat ttctgaagac
     9361 tatactcgct aatggaaaag caaagccgat gaagcgcaat aaattgcgag ataaatctga
     9421 tttgctagta tgcccgcttc ctcactatcg gagttaacac aaggatgaga ttaacgacga
     9481 aattttcggc ctttgttacg ctgctcaccg ggttaacaat ttttgtgact ttgctgggct
     9541 gttcgctaag tttctacaac gccattcagt ataagtttag tcatcgcgtt caggcggtgg
     9601 cgacggcgat tgatacccac cttgtgtcga atgacttcag cgcattaagg ccacaaatta
     9661 ccgaattaat ggtgtcagca gatatcgttc gtgtagacct gctgcatggt gataaacagg
     9721 tttataccct ggccagaaat ggtagttatc gtccggttgg caccagcgat ctgtttcgtg
     9781 aactgagcgt tccgttgata aagcatccgg ggatgtcgtt gcgtctggtt tatcaggatc
     9841 cgatgggcaa ctatttccat tcgttgatga ccaccgcgcc gctcacgggg gcgattggct
     9901 ttatcattct tatgctcttt ctggcggtac gctggttaca acggcaactt gccgggcaag
     9961 aattgctgga aacccgggct actcgtatct taaacggtga gcgtggctct aatgtgttgg
    10021 gaaccatcta tgaatggccg cccagaacca gcagtgcgct ggatacgctg cttcgtgaaa
    10081 ttcagaacgc acgcgaacaa cacagccgtc ttgatacgct gatccgctct tatgccgcac
    10141 aggacgtgaa aaccggcctc aataaccgac tctttttcga taatcagtta gcaacgttac
    10201 tggaagatca ggagaaagta ggtacccacg ggatcgtgat gatgattcgt ctgccggatt
    10261 tcaatatgtt gagcgatacc tgggggcaca gccaggttga agaacagttc ttcactctga
    10321 cgaatctgct gtcgacattt atgatgcgct accctggcgc actgctggcg cgttaccacc
    10381 gcagtgattt tgctgcgctg ttaccgcacc ggacgttaaa agaggcagag agcatagcca
    10441 gtcagttaat caaagccgtc gataccttgc cgaacaataa aatgctcgat cgcgacgata
    10501 tgatccacat tggtatctgt gcctggcgta gtggtcagga taccgagcag gtaatggaac
    10561 atgcagagtc tgccacgcgt aatgcgggat tgcagggtgg caatagctgg gctatttatg
    10621 atgactcgtt gcctgaaaaa ggacgcggta atgttcgctg gcgtacgctt atcgagcaaa
    10681 tgctcagtcg cggcggcccg cgcctttatc aaaaaccggc ggttactcgc gaaggtcagg
    10741 ttcatcatcg cgaactcatg tgccgcatct tcgatggtaa tgaagaggtt agctcggcgg
    10801 agtatatgcc gatggtcttg cagtttggct tatcggaaga gtatgaccgt ctgcaaatca
    10861 gccgtcttat tccactattg cgttactggc cagaggaaaa tctggcgatt caggttaccg
    10921 ttgagtcgct gattcgtccg cgttttcagc gttggctgcg cgatacgtta atgcaatgtg
    10981 aaaaatcaca acgaaaacgc ataattattg aacttgcaga ggccgatgta ggtcaacata
    11041 tcagtcgttt acaacctgtt attcgtttag tgaatgcttt aggggtacgg gtagccgtca
    11101 accaggctgg tttgacgctg gtaagcacca gttggatcaa agaacttaat gttgagttac
    11161 tcaagctcca tccggggctg gtcaggaaca ttgagaagcg aacggagaac cagctgctgg
    11221 ttcaaagcct ggtggaagcc tgctccggga ccagcaccca cgtttacgcc accggcgtgc
    11281 gttcgcgaag cgagtggcag accctgattc agcgcggtgt tacaggcggg caaggggatt
    11341 ttttcgcgtc ctcacagcca cttgatacta acgtgaaaaa atattcacaa agatactcgg
    11401 tttaacctgc cgtttaatcc gttttcacgt agaataacgc gcgctgcgtc tcatgggagt
    11461 gtgcttgtct gctcgccaga ttgttgcagc acatatgcag atgaatgacc ttacgcggtt
    11521 gcaaacaggc gaggaatgct gctgatgcat taagcctttc tggactcagg cagagatttg
    11581 taacaaagga aacgaactgc actaattttc accgtagcag atgatttttg cgccttgtcg
    11641 ctgctgcgtg tggttggtaa agtaagcgga ttttcttttc cgccccagct ttcaggatta
    11701 tcccttagta tgttgaaaaa atttcgtggc atgttttcca atgacttgtc cattgacctg
    11761 ggtactgcga ataccctcat ttatgtaaaa ggacaaggca tcgtattgaa tgagccttcc
    11821 gtggtggcca ttcgtcagga tcgtgccggt tcaccgaaaa gcgtagctgc agtaggtcat
    11881 gacgcgaagc agatgctggg ccgtacgccg ggcaatattg ctgccattcg cccaatgaaa
    11941 gacggcgtta tcgccgactt cttcgtgact gaaaaaatgc tccagcactt catcaaacaa
    12001 gtgcacagca acagctttat gcgtccaagc ccgcgcgttc tggtttgtgt accggttggc
    12061 gcgacccagg ttgaacgccg cgcaattcgt gaatccgcgc agggcgctgg tgcccgtgaa
    12121 gtcttcctga ttgaagaacc gatggctgcc gcaattggtg ctggcctgcc ggtttctgaa
    12181 gcgaccggtt ctatggtggt tgatatcggt ggtggtacca ctgaagttgc tgttatctcc
    12241 ttgaacggcg tggtttactc ctcttccgtg cgcattggtg gtgatcgttt cgacgaagct
    12301 atcatcaact atgtgcgtcg taattacggt tctctgatcg gtgaagccac cgcagaacgt
    12361 atcaagcacg aaatcggttc ggcttacccg ggcgatgaag tccgtgaaat cgaagttcgt
    12421 ggccgtaacc tggcagaagg tgttccacgc ggttttaccc tgaactccaa cgaaatcctc
    12481 gaagcactgc aggaaccgct gaccggtatt gtgagcgcgg taatggttgc actggaacag
    12541 tgcccgccgg aactggcttc cgacatctcc gagcgcggca tggtgctcac cggtggtggc
    12601 gcactgctgc gtaaccttga ccgtttgtta atggaagaaa ccggcattcc agtcgttgtt
    12661 gctgaagacc cgctgacctg tgtggcgcgc ggtggcggca aagcgctgga aatgatcgac
    12721 atgcacggcg gcgacctgtt cagcgaagag taatcggatg caggcagggg aagcgtctgt
    12781 ttaccctgcc tggtctgata cgagaatacg cataacttat gaagccaatt tttagccgtg
    12841 gcccgtcgct acagattcgc cttattctgg cggtgctggt ggcgctcggc attattattg
    12901 ccgacagccg cctggggacg ttcagtcaaa tccgtactta tatggatacc gccgtcagtc
    12961 ctttctactt tgtttccaat gctcctcgtg aattgctgga tggcatatcg cagacgctgg
    13021 cctcgcgtga ccaactagaa cttgaaaacc gggcgttacg tcaggaactg ttgctgaaaa
    13081 acagtgaact gctgatgctt ggacaataca aacaggagaa cgcgcgtctg cgcgagctgc
    13141 tgggttcccc gctgcgtcag gatgagcaga aaatggtgac tcaggttatc tccacggtta
    13201 acgatcctta tagcgatcaa gttgttatcg ataaaggtag cgttaatggc gtttatgaag
    13261 gccagccggt catcagcgac aaaggtgttg ttggtcaggt ggtggccgtc gctaaactga
    13321 ccagtcgcgt gctgctgatt tgtgatgcga cccacgcgct gccaatccag gtgctgcgca
    13381 acgatatccg cgtaattgca gccggtaacg gttgtacgga tgatttgcag cttgagcatc
    13441 tgccagcgaa tacggatatt cgtgttggtg atgtgctggt gacctccggt ctgggcggtc
    13501 gtttcccgga aggctatccg gtcgcggttg tctcttccgt aaaactcgat acccagcgcg
    13561 cttatactgt gatccaggcg cgtccgactg caggactgca acgtttgcgt tatctgctgc
    13621 tgctgtgggg cgcggatcgt aacggcgcta acccgatgac gccggaagag gtgcatcgtg
    13681 ttgctaatga acgtctgatg cagatgatgc cgcaggtatt gccttcgcca gacgcgatgg
    13741 ggccaaagtt acctgaaccg gcaacgggga tcgctcagcc gactccgcag caaccggcga
    13801 caggaaatgc agctactgcg cctgttgcgc cgacacagcc cgctgctaat cgctctccac
    13861 aaagggctac gccgccgcaa agtggtgctc aaccgcctgc gcgtgcgccg ggagggcaat
    13921 agtggcgagc tatcgtagcc agggacgctg ggtaatctgg ctctctttcc tcattgcgct
    13981 gttgctgcaa atcatgccct ggccggataa cctgattgtt ttccggccaa actgggtgtt
    14041 acttatcttg ttgtattgga tcctggcctt gccccatcgc gtaaatgtgg gcacaggttt
    14101 tgtgatgggt gccatactgg atctgatcag cggctcgatg cttggcgtac gcgtattggc
    14161 gatgagcatc attgcttacc tggtggcgct gaaataccag cttttccgca acctcgcatt
    14221 atggcagcag gcgctggtcg tcatgttgct ttcgctggtg gtggatatta ttgttttctg
    14281 ggcagagttt ttagtgatta acgtctcttt cagaccggaa gtgttctgga gtagtgtagt
    14341 caatggggtg ctctggccgt ggattttctt gctgatgcgt aaagtccgtc agcagtttgc
    14401 agtgcaataa aggtttctat gacttctctg tatttagctt ccggttctcc gcgtcgtcag
    14461 gagttacttg cgcaacttgg cgtgaccttt gaacgtattg ttacgggcat tgaggagcag
    14521 cgtcagccgc aggagagcgc gcagcagtat gttgtgcgtc tggcacgcga gaaagcacag
    14581 gcgggtgtcg cgcaaacggc acaggatctc ccggtgctgg gtgcggatat tatcgttatc
    14641 ctgaacggag aagtgctcga gaaaccgcgc gacgcagagc atgcggcgca gatgttgcgc
    14701 aaattatcgg gtcagaccca tcaggtgatg acggcagtgg cgttggccga cagccagcac
    14761 attctcgatt gcctggtggt caccgatgtg actttcagaa cgttaacaga cgaagacatc
    14821 gcgggctatg tcgccagcgg tgaaccgtta gataaagcag gtgcatacgg tattcagggg
    14881 ctgggtggct gttttgtcag gaagataaat ggcagctatc acgccgtagt cggcttaccg
    14941 ctggttgaaa cgtatgaatt attaagtaat tttaacgcac tgcgtgagaa aagggataaa
    15001 catgacggct gaattgttag taaacgtaac gccttcggaa acgcgagtgg cgtatattga
    15061 tggcggtatt ctgcaggaaa ttcatattga acgtgaggcg cgacgcggaa tagtaggcaa
    15121 tatctacaag ggtcgtgtaa gtcgtgtact tccgggtatg caggcggctt ttgtagatat
    15181 tgggctggat aaagccgcgt ttcttcatgc atccgacatc atgccgcata ccgaatgtgt
    15241 ggcgggtgaa gaacaaaagc aattcacggt gcgcgacatc tcggaactgg ttcgtcaggg
    15301 gcaagatctg atggtgcagg tggtgaaaga tccgcttggc actaaaggtg cgcgcctgac
    15361 caccgatatc acgctgcctt ctcgctatct ggtatttatg ccaggggctt ctcacgttgg
    15421 ggtttcccaa cgaattgaaa gcgaatcaga acgtgaacgt ctgaaaaaag tggtcgcaga
    15481 gtattgcgac gagcagggcg ggtttatcat ccgcaccgca gcggaagggg ttggcgaggc
    15541 tgaactggcc tccgatgccg cttatctgaa acgcgtctgg accaaagtta tggagcgtaa
    15601 aaagcgcccg cagacccgtt atcagctgta cggcgaactg gcgctggcgc agcgtgttct
    15661 gcgtgatttt gccgacgccg aactggaccg cattcgcgtt gactcacgcc tgacttacga
    15721 agcattgctg gagtttacct cggagtacat tcccgagatg accagcaagc tggagcatta
    15781 caccggacgc cagccgattt tcgatctctt tgatgtcgaa aacgaaatcc agcgagcgtt
    15841 ggaacgcaaa gtagaactga aatccggtgg ttatctgatt atcgaccaga ccgaagcgat
    15901 gaccaccgtg gacatcaata ccggagcgtt tgtcggtcat cgcaatctgg acgacactat
    15961 tttcaatacc aatattgaag cgacgcaggc tatcgctcgc cagttacggt tgcgtaatct
    16021 gggcgggatt atcattattg atttcatcga tatgaataat gaagatcacc gccgccgcgt
    16081 gctgcattcg ctggagcagg cgttgagcaa agaccgggtg aaaaccagcg ttaatggttt
    16141 ttcggcgctg gggctggtgg agatgacgcg taaacgcacc cgcgaaagca ttgagcacgt
    16201 actgtgtaac gaatgcccaa cctgccacgg tcgcggaacg gtgaaaaccg tggaaacggt
    16261 atgctatgaa atcatgcgcg agattgttcg tgtccaccat gcttacgact ccgaccgttt
    16321 cctggtctat gcttctccgg cagtagccga agccttgaaa ggcgaagagt cacactcgct
    16381 ggcggaagtg gaaattttcg ttggcaaaca ggttaaagta caaattgaac cgctctataa
    16441 ccaggagcag tttgacgtcg taatgatgta aacagatgct gggccgccat ccggcaaagg
    16501 gtttttgagt cacattttta gcagacaagg agtgacgggt gaggcgattg ccggggattt
    16561 tactgcttac tggagccgcg ctcgttgtga tcgctgccct gctggttagc ggcctgcgta
    16621 ttgctttacc gcatcttaac gcctggcgtc cggaaatcct caacaaaata gaatccgcga
    16681 ctggcatgcc ggtagaagcc agtcagctct cagccagctg gcagaatttt ggcccgacgc
    16741 ttgaagcaca cgacatccgt gcagaactaa aagatggcgg cgaattttcg gttaaacgcg
    16801 ttactctggc gctggatgtc tggcagagcc tgttacatat gcgctggcag tttcgcgacc
    16861 tcactttctg gcagctgcgc tttcgcacca acactcctat caccagcggt ggtagcgatg
    16921 acagtctgga agccagtcac atcagcgatc tgtttcttcg tcaatttgac catttcgatc
    16981 tccgcgacag tgaagtcagt tttctgacgc catccggtca gcgcgccgag ctggcgatcc
    17041 cacaactcac ctggctgaac gatccacgtc gacaccgtgc ggaaggcctg gtaagcctct
    17101 ccagccttac cggacagcac ggcgtgatgc aggtgcgcat ggatttgcgc gatgatgagg
    17161 ggttgttaag caatggtcgc gtctggctac aggcggatga catcgacctg aagccgtggc
    17221 tcggtaaatg gatgcaggac aatattgctc tggaaacggc acagttctcc cttgaaggct
    17281 ggatgacgat cgacaaaggc gatgtaaccg gcggtgacgt ctggctgaaa cagggcggtg
    17341 ccagctggtt gggcgagaag caaacgcata cgctgtcggt ggataatctg accgcgcata
    17401 ttacgcgtga aaatccgggc tggcagttct ctattcccga tacacggatc acgatggacg
    17461 gcaaaccctg gccgagcgga gcattgacgc tggcctggat accggaacag gacgttggcg
    17521 gcaaagacaa taaacgcagt gacgaactcc ggattcgcgc tagtaatctg gagctggcag
    17581 gcctggaggg catacgcccg ctggccgcga aactttcacc tgcactgggt gatgtttggc
    17641 gctccacaca accgagcggc aagattaaca ctctggcgct ggatatcccg cttcaggcgg
    17701 cagacaagac ccgttttcag gcatcgtgga gcgatctggc ctggaagcaa tggaaattat
    17761 taccgggtgc ggaacacttc tccgggacgc tttccggcag cgttgaaaat ggtttgctta
    17821 ccgcgtcgat gaagcaggca aagatgcctt acgaaacggt attccgtgcg ccactggaaa
    17881 tcgccgacgg ccaggcaact ataagctggc tgaacaatga caaaggtttc cagctggatg
    17941 ggcgtaatat tgacgttaaa gccaaagccg tccatgcgcg cggcggtttt cgttacctgc
    18001 aacctgctaa cgatgaaccc tggctgggta ttctggctgg catcagtacc gatgatggtt
    18061 cacaagcctg gcgctatttc ccggaaaact tgatgggtaa agacctggtt gattatttaa
    18121 gtggtgcgat tcagggcggt gaagcggata acgcgacgct ggtttatggt ggcaatccgc
    18181 aactcttccc ctataaacac aacgaaggtc agtttgaagt gctggtgccg ctgcgcaacg
    18241 cgaagtttgc cttccagccg gactggcctg cattaactaa ccttgatatt gaactggact
    18301 ttattaacga cggtttatgg atgaaaaccg atggcgttaa tctgggcggc gtgcgcgcga
    18361 gtaatctcac cgcagtgatc cctgactact caaaagaaaa actgctgatt gacgctgaca
    18421 ttaaaggtcc gggtaaagcc gttggccctt actttgatga gacaccgctg aaagattctc
    18481 tgggtgcgac cctgcaagaa ctccagctcg acggcgatgt gaatgctcgc ttacatcttg
    18541 atatcccgct gaacggcgaa ctggtaaccg cgaaaggtga agtgacgctg cgtaataaca
    18601 gtctgtttat caaaccactc gacagcatcc tgaaaaattt gagcggtaaa ttcagcttta
    18661 tcaatggcga tctgcaaagt gaaccactga cagcaagctg gtttaatcag ccgttgaacg
    18721 tggatttttc caccaaagaa ggggcaaaag cctaccaggt tgcggtgaat ctcaacggta
    18781 actggcaacc ggcgaaaacc ggcgttctgc ctgaagcggt gaacgaagca ttgagtggca
    18841 gcgtggcgtg ggatggtaaa gtgggcattg atctgcctta tcatgctggc gcgacctata
    18901 acgtagagct gaacggcgat ctgaataatg tgagtagtca cttaccttca ccgttagcca
    18961 aacctgcggg tgaaccacta gcggtaaacg ttaaggttga tggcaatctc aacagctttg
    19021 aattaaccgg acaggctggt gcggataacc atttcaatag ccgctggttg cttggtcaaa
    19081 agctgacgct cgatcgtgct atttgggcgg cagacagtaa aacgctcccg ccgttgccgg
    19141 aacaaagtgg cgttgaactt aatatgccgc cgatgaatgg tgccgagtgg ctggccctgt
    19201 tccagaaagg cgctgcggag agtgtcggtg gtgcagcgag tttcccacaa cacataacgt
    19261 tacgtacgcc tatgttgtca ctgggaaatc agcaatggaa taacctgagt attgtttcgc
    19321 aaccgacggc aaatggcacc ctggttgagg cgcaagggcg tgaaatcaac gccacgctag
    19381 cgatgcgtaa taacgcgccg tggctggcga atatcaaata tctttattac aacccgagcg
    19441 tggcgaaaac tcgtggtgat tcaacgccgt catcaccttt cccgacaacg gagcgcatta
    19501 acttccgtgg ctggccggac gcacaaatac gatgcacaga gtgctggttc tgggggcaaa
    19561 aattcggtcg cattgacagt gatatcacca tttctggcga tacgttaacg ctgaccaatg
    19621 gactgattga tactggtttc tcgcggctta ctgccgatgg tgaatgggtt aataatccgg
    19681 ggaatgaacg tacctcgctg aaaggaaaac tgcgtgggca gaaaattgat gccgccgcag
    19741 aattttttgg tgtcacgacg cccatacgcc agtcgtcatt taatgtggat tacgatttac
    19801 actggcgcaa agcaccctgg cagccagatg aagcgacgtt gaatggcatc attcatactc
    19861 aactgggtaa aggcgaaatt accgaaatca ataccggaca tgccgggcaa ttgctgcgct
    19921 tattgagcgt agatgccctg atgcgtaagc tgcgttttga tttcagagac acttttggcg
    19981 aagggttcta ttttgactcc attcgcagca ccgcgtggat taaagacggc gttatgcaca
    20041 ccgacgacac gctggtggat ggcctggagg cggatatcgc catgaaaggg tcggtaaatc
    20101 tggtacgccg cgacctgaat atggaagcgg ttgtcgcacc agagatttct gcgacggtgg
    20161 gcgtggctgc ggcttttgcg gttaacccca ttgttggcgc ggcagtgttt gccgccagta
    20221 aagtgctggg gccgctgtgg agcaaagtct ccattttgcg ctatcacatt tcgggtccgc
    20281 tggacgatcc gcaaatcaac gaagtgttgc gccaaccgcg taaagaaaaa gcgcaatgat
    20341 ttgacgaggg cgcgtaattg ccccaatctc atatgataat cgttgccaaa ggccaacgag
    20401 ccggaacata actgtaggcc ggatagggcg ttcacgccgc atccggcagc cgtaaaaaat
    20461 cctctactgc agtaactaac gagtagcaaa aacgatgagt ctgaacctgg taagtgaaca
    20521 attgctagcg gcgaacggcc tgaaacatca ggacttgttc gcgatcctcg gtcaactggc
    20581 cgaacgtcgc cttgattatg gcgatctcta ttttcagtcg agctatcacg aatcctgggt
    20641 tttagaagac cgcattatta aagatggttc ttacaacatc gatcagggcg ttggcgtgcg
    20701 tgcaatcagc ggtgaaaaaa ccggatttgc ttacgctgac caaatcagcc tgctggcgct
    20761 ggaacagagt gcgcaagcgg cgcgcaccat cgtccgtgat agtggtgatg gcaaagtaca
    20821 gacgctgggc gcggtagagc atagcccgtt gtatacctcg gtagatccgc tgcaaagcat
    20881 gagccgtgaa gagaagctgg atatcctgcg tcgcgtcgat aaggttgctc gcgaagcgga
    20941 caagcgcgta caggaagtga ctgccagcct cagtggtgtc tatgaattaa ttctggttgc
    21001 ggccaccgac ggcacgctag cggcggatgt ccgtccgctg gtgcgtcttt ccgtgagcgt
    21061 tctggtcgaa gaagatggca aacgcgaacg cggtgccagt ggcggcggcg gtcgttttgg
    21121 ttatgagttc ttccttgccg atcttgacgg cgaagtccgt gcggatgcat gggcaaaaga
    21181 agcagtgcgt atggcgctgg tcaatctttc tgccgttgct gcaccagcgg gcaccatgcc
    21241 ggtagtactt ggcgcaggtt ggccgggcgt gctgttgcat gaagcggtag gtcacggtct
    21301 ggaaggcgac ttcaaccgcc gtggcacttc agtatttagt ggacaggtcg gggagctggt
    21361 ggcttcagaa ctgtgtaccg tggttgatga cggcacgatg gtcgatcgcc gtggttcggt
    21421 ggcgattgat gatgaaggta cgccaggcca gtacaacgtg ctgattgaga acggcattct
    21481 gaaaggctac atgcaggata aactcaacgc gcgtttgatg gggatgacgc cgactggcaa
    21541 cggtcgccgt gaatcctacg cccatctgcc catgccgcgt atgaccaaca cctatatgct
    21601 gccgggtaaa tcgaccccgc aagaaattat tgaatccgtt gagtacggta tctatgcacc
    21661 gaactttggt ggcggtcagg tggatatcac ctccggcaaa ttcgttttct ccacttcaga
    21721 agcatatctg attgaaaacg gtaaagtaac gaagccggtg aaaggcgcaa cgttgattgg
    21781 ttccggtatc gaaaccatgc agcagatttc gatggttggc aacgacctga aactggataa
    21841 cggcgtgggt gtctgcggta aagaagggca aagtttgccg gttggcgtgg gccagccaac
    21901 gttgaaagtc gataacctga ctgttggcgg tactgcgtaa taatttcatt attttcagaa
    21961 ggataattaa tctttctacg tgcacgaacg gtcccctcgc tcctctgggg ttagggtgag
    22021 gggaacccgt tggcacaggt ttgtagaacg taacagtaca atatgaatta cttctctttc
    22081 ccgcgcccgt gcatctcctg aaacaattta ccgacctcaa caaaataatc cgtcagcgag
    22141 ttgatcacga cctgtacctt cagcggcagc ttatcttttt cggtatataa cgcataaacc
    22201 gggcgtggat ctgactgata acgcggcagc aggatctcca gctccccacg attgatctcg
    22261 ttgatcaccc acatcagcgg cacgtaggcg atcccggcac ccgccgttag ccagcgcacc
    22321 agcgtcatcg gatcattagt cacaaatctc ccttgcggga tcaggcgagt cgagatccct
    22381 tccggtgcaa tcagttcaaa ttcattgtcg ggccgcacgc tgtattcaag ccatgaatgg
    22441 ctacttaaat cggcgggttt ttccggtatg ccgtattgtg tgagatagct tttcgcggcg
    22501 cacaccacca ttggcatcgc gccaagacgg cgggaaaaca ggctggaatc ctgcaacgcg
    22561 ccgacgcgga tcaccacatc cagaccgtcg gcaatcaggt cgggggctgg aattccggta
    22621 accagattgg cgctcaaacc tgggtattct ttcagcattt tggctgtcag cccggcgaga
    22681 acattttgtg ccatagttga agaacagcca atgcgtagcg tcccgatggg ggtgttattg
    22741 aaggcataca gttgctcatg aacatcctgc acttcatgaa gcatacgacg gcagccctgg
    22801 tagtaaattc taccggcttc ggtcaggcca atgctgcgtg tgctacggtt taacagcttt
    22861 acctgcaact catcttccag ttttgacacc gtctgactga tggacgaaac gctcatctgt
    22921 agctgtctgg cggcggcggt aaaagagcca aattcaacta ctttggcaaa caccgacatg
    22981 cgttttagtc gttccattat tcactctgac ttaaaagtga tttagatcac ataatataga
    23041 taacagcata acagttacgc taatatatta aatatcaatc tacagcaatg ttgctctcgc
    23101 ccggctttcc atgcccttct ctgcggcgac agatgctgaa aataataacg cctgctctct
    23161 ctatacaacc aaggtcaaca tgagtctgtt tcccgttatc gtggtgtttg ggctgtcctt
    23221 cccaccgata ttttttgaat tgcttttatc actggcgatt ttctggctgg tgcgccgggt
    23281 acttgtgcca acaggtatct acgactttgt ctggcatccg gcgttgttca acaccgcgct
    23341 ctattgctgc ttgttttatt tgatatcgcg actgttcgtt tgaggttgaa gtgaaaacac
    23401 taataagaaa attctcccgt acggccatca cggtcgtatt agtcattctg gccttcatcg
    23461 caatttttaa tgcctgggtc tattacaccg aatccccctg gacgcgtgac gcgcgcttta
    23521 gcgctgacgt cgttgcgatc gcgccggacg tttctggact cattacccag gtgaatgttc
    23581 atgataacca gctggtgaaa aaaggacaga tactgttcac catcgaccag ccgcgctatc
    23641 aaaaggcgct tgaggaagcg caagccgatg ttgcttatta tcaggtactg gcacaggaga
    23701 aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca ggcgatgtct cgcgaagaga
    23761 tcgaccaggc taacaacgta ctacaaacgg ttctgcatca gttagcgaaa gcgcaggcga
    23821 cccgcgatct ggcaaaactg gatcttgaac gcacggtgat ccgagcgcca gcagatggct
    23881 gggtgaccaa cctcaacgtc tataccggtg agtttattac tcgaggatca acggcggttg
    23941 cgctggtgaa acagaactcc ttctatgtac tggcctatat ggaagaaact aagctggaag
    24001 gggtgcgtcc ggggtatcgt gcagagatca cgccgcttgg cagtaacaaa gtgctgaaag
    24061 ggactgttga tagtgttgcc gctggggtca ccaacgccag cagcacgcgt gacgacaaag
    24121 ggatggcgac catcgactct gaccttgaat gggtgcgttt ggcgcaacgt gttccggttc
    24181 gtattcgtct cgacaaccag caagagaaca tctggcctgc gggcaccact gctacagtgg
    24241 tggtcactgg caaacaagat cgcgacgaaa gccaggattc gttcttccgt aaaatggccc
    24301 atcgcctgcg tgagtttggt taatcacgat gggtattttc tccattgcta accaacatat
    24361 tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg ctgtttgttg gctttcactt
    24421 ccagctggaa acgccacgct gggcggtact gacagcggcg attgttgccg ccggtccggc
    24481 ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc tatcgtggct ttttgcgcat
    24541 catcggcaca tttattggct gtattgccgg actggtgatc atcattgcga tgatccgcgc
    24601 accattattg atgattctgg tgtgctgtat ctgggccggt ttttgtacct ggatatcctc
    24661 gctggtacga atagaaaact cgtacgcgtg ggggctggcc ggttataccg cgctgatcat
    24721 tgtgatcacc attcagccgg aaccattgct tacgccgcag tttgccgtcg aacgttgtag
    24781 cgagatcgtt atcggtattg tgtgtgcgat tatggcggat ttgctctttt ctccgcgatc
    24841 gatcaaacaa gaagtagatc gagagctgga aagtttgctg gtcgcgcaat atcaattaat
    24901 gcaactctgt atcaagcatg gcgatggtga agttgtcgat aaagcctggg gcgacctggt
    24961 gcgacgcacc acggcactac aaggcatgcg cagcaacctg aatatggaat cttcccgctg
    25021 ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta tcgctgacgc tgattaccca
    25081 atcctgcgaa acttatctta ttcagaatac gcgcccggaa ttgatcactg atactttccg
    25141 cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc cacaagcagc tcaaacgcct
    25201 gcggagagtt atcgcctgga ctggggaacg ggaaacgcct gtcaccattt atagctgggt
    25261 cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt atcagtaaca caaaaatcaa
    25321 cgccaccgaa gaagagatcc tgcaaggcga accggaagta aaagtagagt cagccgaacg
    25381 tcatcatgca atggttaact tctggcgaac cacactttcc tgcattctgg gcacgctttt
    25441 ctggctgtgg acgggctgga cttccggcag tggtgcaatg gtgatgattg cggtcgtgac
    25501 gtcactggca atgcgtttgc cgaatccacg catggtggcg atcgacttta tctatgggac
    25561 gctggccgcg ctgccgttag ggctgctcta ctttttggtg attatcccta atacccaaca
    25621 gagcatgttg ctgctgtgta ttagcctggc agtgctggga ttcttcctcg gtatagaagt
    25681 acagaaacgg cgactgggct cgatgggggc actggccagc accataaata ttatcgtgct
    25741 ggataacccg atgactttcc atttcagtca gtttctcgac agcgcattag gacaaatcgt
    25801 cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg gataaatcgc gcgacaggac
    25861 tggacgtgta ctgcttaatc agtttgtttc tgccgctgtt tccgcgatga ctaccaatgt
    25921 ggcacgtcgt aaagagaacc acctcccggc actttatcag cagctgtttt tgctgatgaa
    25981 taagttccca ggggatttgc cgaaatttcg cctggcgctg acgatgatta tcgcgcacca
    26041 gcgcctgcgt gatgcgccga tcccggttaa cgaggattta tcggcgtttc accgacaaat
    26101 gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat gataaacgtc gtcggtactt
    26161 tggccagttg ctggaagaac tggaaatcta ccaggaaaag ctacgcatct ggcaagcgcc
    26221 accgcaggtg acggaaccgg ttcatcggct ggcggggatg ctccataagt atcaacatgc
    26281 gttgaccgat agttaagtca aaaccgacgc caaaagcgtc ggttttttca tggctatact
    26341 tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg gcaacagatg aatatttatg
    26401 cctttgattt tgatgagatt gagagtcagg aggattttta tcgtgacttt agccaaacct
    26461 ttggtctggc taaagataag gtacgcgatc tcgactcact atgggatgtg ttaatgaacg
    26521 atgtcctgcc gctaccactt gagattgaat ttgttcatct gggagagaaa acgcgtcgcc
    26581 gttttggcgc gttaatattg ctgtttgatg aggcagagga agagctggaa gggcatttgc
    26641 gttttaatgt tcgtcattag cgcaaaaaaa agcccacgaa ccgggggcaa tatcgtcgga
    26701 caaaacgatg agggtttatt tgtacagttc agccgtagcg tgccaggtgt caccgctacg
    26761 agcttcagta atctggtagg ccgttgcgcc tttctcttcc gcttttttgt tcagcatttc
    26821 acgtatatcc attggcgaag acgccacacc acttacggat acggtcccga ttgcttcacg
    26881 attttgtgct tgtgcagcat caatggagtc ggcagcgaat gcaccgaaag agagaacaga
    26941 aagtacgctt aatgcagcta cagtggtttt gattttcatg atttttacct cgacataatc
    27001 ttttagctgg gcctttgttt cgtgacccat atcacaaaat caagtataca ctaattattg
    27061 aactaattaa taccacacta actgtttcag ttgatactaa gtgttaaaaa tgtgaatttt
    27121 aataacaaaa aagtataaaa aacacggtta tttattatta attatagtat tttcaattag
    27181 ataatgaatt ttgcataatg tgcttttttt accgtatgta ttcattttgt gaatgatgtg
    27241 tcgcggtaaa acgcactatt cgttaaatgt atcgcaggag aaagaagggg aatgagaggg
    27301 ataagcaaca ttttccccgc cgccagaagc gacggggcag agattaaagc tcctggtcga
    27361 acagctctaa aatcgcttcg tacaggtctt tgacggtgaa accgttagca ggggtggtaa
    27421 agatggtgtc atcgccagcg atggtgccca gaataccttc tgctttgccc agtgagtcca
    27481 gcaggcgagc aattaactgc gccgcgccag ggctggtatg aatcacgaca actgcatcgt
    27541 tgtagtcgat atccagcacc agattcttca atggactgga ggtggttggt acacccagtt
    27601 cagctggcag gcagtaaacc atttccattt tggcattgcg tgtacgtaca gcaccaaact
    27661 tggtcaacat ccgcgagact ttagactgat taatattgtc aaagccttgc tcctgcaacg
    27721 cggcgacgat ttcgccctgg gagctaaatt tctcttcttt aagtaatgct ttaaatgctt
    27781 taactagttc ttcttgctta gccgagcttc gcataagtca cccgatatgg tggttgatac
    27841 aacattattg tgcatacaga tgaattttta tgcaaacagt cagccctgaa gaaggctgaa
    27901 ataatgttat gaaagagcgg gattttatca aatttcgtta ttgagaaaca tgcctgcgtc
    27961 acggcatgca aattctgttt aaaagtaaat taattgttat caaattgatg ttgttttggc
    28021 tgaacggtag ggtatattgt caccacctgt tggaatgttg cgctaatgca taagcgactg
    28081 ttaattacgt aagttaggtt cctgattacg gcaattaaat gcataaacgc taaacttgcg
    28141 tgactacaca ttcttgagat gtggtcattg taaacggcaa ttttgtggat taaggtcgcg
    28201 gcagcggagc aacatatctt agtttatcaa tataataagg agtttaggat gaaagtcgca
    28261 gtcctcggcg ctgctggcgg tattggccag gcgcttgcac tactgttaaa aacccaactg
    28321 ccttcaggtt cagaactctc tctgtatgat atcgctccag tgactcccgg tgtggccgtc
    28381 gatctgagcc atatccctac tgctgtgaaa atcaaaggtt tttctggtga agatgcgact
    28441 ccggcgctgg aaggcgcaga tgtcgttctt atctctgcag gcgtagcgcg taaaccgggt
    28501 atggatcgtt ccgacctgtt taacgttaac gccggcatcg tgaaaaacct ggtacagcaa
    28561 gttgcgaaaa actgcccgaa agcgtgcatt ggtattatca ctaacccggt taacaccaca
    28621 gttgcaattg ctgctgaagt gctgaaaaaa gccggtgttt atgacaaaaa caaactgttc
    28681 ggcgttacca cgctggatat cattcgttcc aacacctttg ttgcggaact gaaaggcaaa
    28741 cagccaggcg aagttgaagt gccggttatt ggcggtcact ctggtgttac cattctgccg
    28801 ctgctgtcac aggttcctgg cgttagtttt accgagcagg aagtggctga tctgaccaaa
    28861 cgtatccaga acgcgggtac tgaggtggtt gaagcgaaag ccggtggcgg gtctgcaacc
    28921 ctgtctatgg gccaggcagc tgcacgtttt ggtctgtctc tggttcgtgc actgcagggc
    28981 gaacaaggcg ttgtcgaatg tgcctacgtt gaaggcgacg gtcagtacgc ccgtttcttc
    29041 tctcaaccgc tgctgctggg taaaaacggc gtggaagagc gtaaatctat cggtaccctg
    29101 agcgcatttg aacagaacgc gctggaaggt atgctggata cgctcaagaa agatatcgcc
    29161 ctgggcaaag agttcgttaa taagtaattg attagcgaat aataaaaaac cggagcacag
    29221 actccggttt ttgttttgag cactcgactt aattggttgc cggatattcc tgaatggtga
    29281 cctgcagcgt taactgctta tcatcacgca tcactacaac cgggatcacc gaaccagggc
    29341 gaatttccgc cacctgatcc atcgtctcca gagcagagat ggccggtttg ttatccaccg
    29401 aaataatcag atcgttgacc tgaatacccg cattcgccgc tgggccgtca ggtgacactt
    29461 cattaaccac gatcccttgc agttgatcta taccaccgct ctgcgcgtgc agtggtgcga
    29521 tctcgcgtcc gccgatacca atgtagccgc ggatcacgcg accatcgcgg atcagcttat
    29581 ccataatttt ggttgctaac tggaaaggaa tcgcaaagcc gataccttcc ggcgtttcgc
    29641 catcgttact cttatcaaac gacagcgtgt taatgcccat cagttctccc agcgagttca
    29701 ccagcgcgcc gccagagtta ccgtggttaa tggaagcatc ggtttgtagg aagttttgcc
    29761 gcccggtcgg gttcagaccg attcgacccg tggcactaat aatcccttgg gtaatggtct
    29821 gcccgaggtt gtacgggtta ccgatcgcca gtactacgtc gccaatgtgc ggtacgcgac
    29881 gtgcattaat tggaatggta ggtaaaccgc cagtggcatt aattttaagt accgccagat
    29941 cggttagaga gtcagatccc accagcaatg cttcaaatac acgtccatcc tgtaaggcga
    30001 cgatgatctg atcggcgtcg ttgatgacgt gtttattggt gatgatataa ccgcgttgat
    30061 ccatgattac accggatccc agggtgcgga tctcaagctg gttgtgagag ttggtgttca
    30121 aaccacggtt gtaaacgtta accaccgctg gcgcggcgcg gcgaaccgcc agattatagc
    30181 tggcaggcgt ctcatcggta ctgtcaaatt gcggagtgga aagcgggtta aggctgcgca
    30241 gcgaaggcat ggcaaccagc agaatagcgc cgacaattaa tccaatcgca acggaacgta
    30301 agagcttcac aaacatgatg gaggcgtcat taaaaaaggg aacggcagca gcataccacg
    30361 agttaaccgg acatcacacg tcagcctgat gcccggttta cgacattaac gcatcagcag
    30421 atagatgctt tcattgccgc gtacaatttg cagggcgatg atggccggtt ttgccgccag
    30481 cactttacgc atttcagcaa tcgagttcac ccgatcgcgg ttgacgccaa tgatcacatc
    30541 gtctttttgc aagccagcct gagcagctgg gcttcctttg acaacttcat ctatcttaat
    30601 acctttgccg ccatctttta gctgaccatc gctcaacgtt gcaccttcca gcgctggccg
    30661 acgaagaggt gctggtatca agcgtcactt ctacttccag tggtttgccg ttacgcagca
    30721 ggccaagctt cactttcgtg cccggctctg tagtcgcgat acgagagcgc aactcagcaa
    30781 agctattcag cggtttgccg ttgaggctgg taataatatc gcccgctttg acgcccgctt
    30841 tcgctgagcc agaacttggc aacacttcgc tgacaaacgc gccacgctgc acgtcaaggt
    30901 tgaaggcttt ggcgatatcg gcactcatct cggtgccttt gatgcctaac aaaccgcgtt
    30961 tgatttcacc aaagtcgata agctgctgcg ccagtgttcg cgccatatta ctggggatgg
    31021 caaatccaat cccgacgctc ccgccgccag gcgcaaggat tgcagtgttg atgccaatta
    31081 actcaccgtt aaggtttaat agtgcaccgc cggagttacc gcggttaatg gaagcatctg
    31141 tctggataaa gttttccaga ccttcaagat tcaacccgct gcggcctaat gcggaaacaa
    31201 tgccagaggt ggcggtttgc ccaaggccaa atgggttacc gaccgctacg gcaaaatcac
    31261 cgacgcgcaa tttatcggag tcggcaatag cgatttgcgt taatttgctc gggttttgaa
    31321 tttgtaacag ggcgatatcg ctctggtcat cgctaccaat cagttttgca tcaaactcgc
    31381 gcccatcatt gagctgaata ctgattttct gtgcctgatt aatcacatgg ttgttggtca
    31441 gcacatagcc tttactggcg ttgatgatga caccggaacc taaaccttcg aagggttgtg
    31501 caggttgatc cggtaaatca tcaccaaaaa actttttgaa ttcttccggg attttctgtc
    31561 cctgactggc cgttccttcc acccgtacgc tcaccactgc cggaagcact ttttccagca
    31621 ttggagccag actggggaga ggggcctgat cggcaacctg gcctggaatc gacgcgacgg
    31681 cctgaaatga cgccgagaga gttaacccgg cacttaacgc taatgcactc aacagctggg
    31741 tttgtttttt cattattcct gctctcgtac ctgaatgata agaaaagaga ttcaaaacag
    31801 tttgatgtta ttgaattttc agacggctaa caatgagaca ccagattaaa tgatagtcgg
    31861 ggagggagag aagaggaggg cgcaatggct gcgcccgaaa aataaattag tcgcgcttcg
    31921 cgccagtacg cagcaggccg gatgcgcctt cagaatagtc gcgaggcacc tgcaccggtg
    31981 cctgatcgtt gctggcttca gactctgcca gacgattacg gaacgggttt gcttcagcag
    32041 acagttccgg cagcaggctg ctggagcttt ttgccatgtg ctgatacagc tggcgatagt
    32101 cgtgcgccat ggtatccagt aattccgcgc tgcgggcaaa gtggctaacc agctcttcgc
    32161 gatactcgtc cagttcagct ttattctttt ccagttcgta ctgcaacgcc tgttgctggc
    32221 gtagtttacg attaccaaaa cgcatggcca cagcaccaat aatgatgccg acgactaacc
    32281 caattagcgc atattcccag gtcatgaaca tctcccgttg tcttttgttt ccgtagggtg
    32341 ttggcttcag gctccatgcc tgcggctgat tatgccacta taaccgttaa ttccacagaa
    32401 gtggaatccc gactgcatat cgcgtagtgt agaacggcct ttttttcgtc aaccgtgaac
    32461 aacggcgcac cgattattca aggaataaca ataagatcat gcaaagcgtt accccaacat
    32521 cgcaatacct gaaggcgctc aatgaaggca gccatcaacc cgacgacgtt caaaaagagg
    32581 ccgtcagccg cctggaaatt atttatcaag aactcatcaa tagcaggcca ccagccccca
    32641 ggacgagtgg gctaatggcg cgggtcggta agctgtgggg taaacgcgaa gacacaaagc
    32701 atacgccagt gcgtggctta tatatgtggg gcggtgtagg acgcgggaaa acctggctga
    32761 tggacctttt ctatcaaagc ctgccgggag agcggaaaca gcgcctgcac tttcaccgtt
    32821 ttatgctgcg ggtgcacgaa gagctaactg ccttacaggg gcagaccgat ccgctggaaa
    32881 ttattgccga tcgctttaaa gccgaaactg acgtgctctg ttttgacgaa ttttttgttt
    32941 ctgatattac cgacgccatg ctacttggcg gtctgatgaa agccctgttc acccgcggta
    33001 ttaccctggt agcgacgtca aatattccgc cggacgaact ttatcgaaat ggcctgcaac
    33061 gtgcgcgttt tctgcctgca atcgatgcca ttaaacagca ttgtgatgta atgaacgtgg
    33121 acgctggtgt tgattatcga ctgcgtacac tcactcaggc gcatctgtgg ctttcgccac
    33181 tcaacgatga aacccgggcg cagatggata aactatggtt ggcgctggcg ggggtgaaac
    33241 gagaaaattc accgacgtta gaaatcaacc atcggccatt ggcgacaatg ggcgtcgaga
    33301 accagacgct ggcggtttct tttactacgc tgtgcgtcga cgcccgcagt cagcatgact
    33361 atattgcgct ctcacgcctg tttcacacag taatgttgtt tgatgtgcca gtcatgacgc
    33421 ggttgatgga gagcgaagcg cggcgcttta ttgcgctggt ggatgagttt tacgagcgcc
    33481 atgtcaaatt agtggtgagt gcagaagtgc cgctgtatga aatttatcag ggtgagcggc
    33541 tgaagtttga gttccagcgt tgcctgtcac gtctgcaaga gatgcaaagc gaagagtatc
    33601 tgaagcgcga gcatttggcg ggttaaaacc tgccacaaat cacaaaaagg ggtcgatctt
    33661 tgaccccgac ttctctataa tcctgcgacc ccacgttaca agaaagtttt tttcccaaaa
    33721 ctttttgtgt gctggcatag gctattcgaa ggggtaggtt tgccggactt tgtcgtgtga
    33781 acctcaacaa ttgaagacgt ttgggtgttc accaacgtgt aactatttat tgggtaagct
    33841 tttaatgaaa acttttacag ctaaaccaga aaccgtaaaa cgcgactggt atgttgttga
    33901 cgcgaccggt aaaactctgg gccgtctggc tactgaactg gctcgtcgcc tgcgcggtaa
    33961 gcacaaagcg gaatatactc cgcacgtaga taccggtgat tacatcatcg ttctgaacgc
    34021 tgacaaagtt gctgtaaccg gcaacaagcg tactgacaaa gtgtactatc accacaccgg
    34081 ccacatcggt ggtatcaaac aagcgacctt tgaagagatg attgctcgcc gtcctgagcg
    34141 tgtgattgaa atcgcggtta aaggcatgtt gccaaaaggc ccgctgggtc gtgctatgtt
    34201 ccgtaaactg aaagtttacg cgggtaacga gcacaaccac gcggcacagc aaccgcaagt
    34261 tcttgacatc taatcgggat tataggcaat ggctgaaaat caatactacg gcactggtcg
    34321 ccgcaaaagt tccgcagctc gcgttttcat caaaccgggc aacggtaaaa tcgtaatcaa
    34381 ccaacgttct ctggaacagt acttcggtcg tgaaactgcc cgcatggtag ttcgtcagcc
    34441 gctggaactg gtcgacatgg ttgagaaact ggacctgtac atcaccgtta aaggtggtgg
    34501 tatctctggt caggctggtg cgatccgtca cggtatcacc cgcgctctga tggaatacga
    34561 cgagtccctg cgttctgaac tgcgtaaagc tggcttcgtt actcgtgacg ctcgtcaggt
    34621 tgaacgtaag aaagtcggtc tgcgtaaagc acgtcgtcgt ccgcagttct ccaaacgtta
    34681 attggcttct gctacggcag aaaacaattt gcgaaaaaac ccgcttcggc gggttttttt
    34741 atggctaaat tctgaatcag cgtaaaaaat gggaagttgc tttttgctgc cgtctgaaag
    34801 acaggtaaaa caaaaaccac atcgccaata agggtctaag tcaactattt cagactaaag
    34861 cgcatctctt tttccccatt tccggcatcc actcaccaca aaggtcgcaa aatctggtaa
    34921 actatcatcc aattttctgc ccaaatgtcg ggtattgctc attttttgtt tgattttcga
    34981 acaaagagag tcgttcctta ttgggtaaca caacttctga ctggccacct ggtggctggt
    35041 agcagtaaaa attctgacta tacctggagg ttttcatggc tgtcgctgcc aacaaacgtt
    35101 cggtaatgac gctgttttcc ggtcctactg acatctatag ccatcaggtc cgcattgtgc
    35161 tggctgagaa aggtgtaagt ttcgagatcg aacacgtgga aaaggacaat ccgcctcagg
    35221 atctgattga cctcaacccg aatcagagcg ttccgaccct ggtggatcgt gagctgaccc
    35281 tgtgggaatc tcgcatcatt atggaatatc tggatgagcg tttcccgcat ccgccactga
    35341 tgcctgttta cccggtagct cgcggtgaaa gccgtctgta catgcatcgc atcgaaaaag
    35401 actggtacac gctgatgaac actatcatca acggttcagc ttctgaagca gatgccgcac
    35461 gtaagcaact gcgcgaagaa ctgctggcga ttgcgccggt cttcggtcag aagccgtact
    35521 tcctgagcga tgagttcagc ctggtcgatt gctaccttgc tccgctgctg tggcgtctgc
    35581 cgcaactggg catcgagttc agcggcccgg gtgcgaaaga gctgaaaggc tatatgaccc
    35641 gcgtctttga gcgtgactct ttccttgctt ctttaactga agcagaacgt gaaatgcgtc
    35701 tgggccggag ttaatctgta tggatttgtc acagctaaca ccacgtcgtc cctatctgct
    35761 gcgtgcattc tatgagtggt tgctggataa ccagctcacg ccgcacctgg tggtggatgt
    35821 gacgctccct ggcgtgcagg ttcctatgga atatgcgcgt gacgggcaaa tcgtactcaa
    35881 cattgcgccg cgtgctgtcg gcaatctgga actggcgaat gatgaggtgc gctttaacgc
    35941 gcgctttggt ggcattccgc gtcaggtttc tgtgccgctg gctgccgtgc tggctatcta
    36001 cgcccgtgaa aatggcgcag gcacgatgtt tgagcctgaa gctgcctacg atgaagatac
    36061 cagcatcatg aatgatgaag aggcatcggc agacaacgaa accgttatgt cggttattga
    36121 tggcgacaag ccagatcacg atgatgacac tcatcctgac gatgaacctc cgcagccacc
    36181 acgcggtggt cgaccggcat tacgcgttgt gaagtaatac aaaacaggcc caggcggcct
    36241 gttttgtctt tttaatgact taataaaata gcgtgattgt ggcaatcata ttcaccacga
    36301 aaccgactgc catggggatc gctgtccgct tcaccaccgc aaaaggcgaa acgcctgcaa
    36361 ttcctgacgt aacgaccacc accgcagtaa tcggcgaaac cgcgcgcgct agcgtcgtgg
    36421 caaaatgcat cggcataatc attacaaccg ctggtacatg tagtccggct gcgatattcg
    36481 gaataagact ggcaaatggc ataaacggcg cattgccaga gcccatcaca atggcacaaa
    36541 tggcaatgac tagcgccata ataatcatca cgccaatacc gcccagacca gaatgctccg
    36601 cacccctgat aaccgcatca acagtgccaa tcgtcgttaa gcctttcgca aatatttcac
    36661 ccgcgaccac cagcgttacc acgttgacaa actgcgtacc catgccgtca aaaaacgcct
    36721 gcacatcgtc cattgtttcg cgcaagttat gcttgcggaa gaactcaaca aacatcgtca
    36781 ccgtcaaact tagtaacatc accaccacca gatgcagttc tgactgcatc agcccgacgt
    36841 gggcgaggaa cagcgagccg agcatcagga ttaacggcat cacaggtaaa atggcgtaat
    36901 agagcggcgg gacattatcg agagtttttt gctctgcctg ttcgtgattg atatttttat
    36961 cttttttgtc aaaagcgcgt tgcacgaaaa agtgggagat cgccaccgag ataatgacgc
    37021 aagaggcgac cggaagctgg tagtggaaga agtatgtggc aattttcatt cccgcgacct
    37081 gggctgcaaa aatggagttc gtttccagaa tcccccactc aatggacatc gtggttgcga
    37141 taaccgccac cgcagagagg cgacttactc ccagactcac cagcgtcgga aataaggtga
    37201 ccatcagcaa catacccaga ccggaggcgc tggtaataaa ctgcgccatg atttggccga
    37261 tgacgtaagt tgccgacaga ataatatacg gcgagcgaat gagttttaac gggcggctta
    37321 gcaagctcac catcgcgcaa ctggctccta tgcgctccat gtagcgggca taaccgccca
    37381 ccgccataat cgacagcccc aatccggcaa tgcggttgga cagcatgcgc aggatttcat
    37441 tataaatgtc gacgatcaag tagccgctgc ttttggcggg atcgagtaca ccaccgaacc
    37501 ctaaccagac tccgcacatc atcaggaaga cacctgcggc agccagcacc acctgaggtt
    37561 tgtagttttt caggatcaaa tagcccatcg taattaatac gatgacagat ataattatgc
    37621 cgaacatt
//
LOCUS       DAJTRD010000030        36597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTRD010000030 DAJTRD010000000
VERSION     DAJTRD010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.030"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(156..1391)
                     /locus_tag="OO315_001699"
     CDS             complement(156..1391)
                     /locus_tag="OO315_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5890647.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="OO315_001700"
     tRNA            complement(1538..1614)
                     /locus_tag="OO315_001700"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="OO315_001701"
     tRNA            complement(1657..1743)
                     /locus_tag="OO315_001701"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="OO315_001702"
     tRNA            complement(1764..1839)
                     /locus_tag="OO315_001702"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="OO315_001703"
     tRNA            complement(1898..1974)
                     /locus_tag="OO315_001703"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="OO315_001704"
     CDS             complement(2077..3462)
                     /locus_tag="OO315_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5890648.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO315_001705"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO315_001705"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5890649.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO315_001706"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO315_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5890650.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO315_001707"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO315_001707"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5890651.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO315_001708"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO315_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5890652.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO315_001709"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO315_001709"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5890653.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO315_001710"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO315_001710"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5890654.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO315_001711"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO315_001711"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5890655.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO315_001712"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO315_001712"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5890656.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO315_001713"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO315_001713"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5890657.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO315_001714"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO315_001714"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5890658.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO315_001715"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO315_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5890659.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO315_001716"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO315_001716"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5890660.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO315_001717"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO315_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5890661.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO315_001718"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO315_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5890662.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO315_001719"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO315_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5890663.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="OO315_001720"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="OO315_001720"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5890664.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="OO315_001721"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="OO315_001721"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5890665.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO315_001722"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO315_001722"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5890666.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="OO315_001723"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="OO315_001723"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5890667.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO315_001724"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO315_001724"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5890668.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="OO315_001725"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="OO315_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5890669.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO315_001726"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO315_001726"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5890670.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO315_001727"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO315_001727"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5890671.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO315_001728"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO315_001728"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5890672.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO315_001729"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO315_001729"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5890673.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="OO315_001730"
     CDS             complement(31267..32913)
                     /locus_tag="OO315_001730"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5890674.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="OO315_001731"
     CDS             complement(32916..32966)
                     /locus_tag="OO315_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890675.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO315_001732"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO315_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5890676.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="OO315_001733"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="OO315_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="OO315_001734"
     CDS             complement(35006..35344)
                     /gene="maoP"
                     /locus_tag="OO315_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5890677.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35463..36302
                     /gene="hdfR"
                     /locus_tag="OO315_001735"
     CDS             35463..36302
                     /gene="hdfR"
                     /locus_tag="OO315_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5890678.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36398..36473)
                     /locus_tag="OO315_001736"
     tRNA            complement(36398..36473)
                     /locus_tag="OO315_001736"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36438..36440),aa:Trp,seq:cca)
     gene            complement(36482..36558)
                     /locus_tag="OO315_001737"
     tRNA            complement(36482..36558)
                     /locus_tag="OO315_001737"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36522..36524),aa:Asp,seq:gtc)
BASE COUNT         8944 a        10015 c         9218 g         8420 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaagc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatccca ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaaagggc atttcgccct ttttattaat cgtcagaatc ggtgtagtct tcagcacctt
    35041 caacctgcgg tttaccgccg gaaagggtgt gaaaacgttt tggacgctta atacgcgtca
    35101 tatacttgga ccacacgcgt tctgcttctg tcactggctc acgttcgcca cggcatactg
    35161 ctacaaagag tttctcttcc tcggtaaccg gctcgcgttt gccaagatcc aactcattga
    35221 aggcataacc atgacgctca agcagttgtg cctctttgat ggtgaaatca ccatgacgag
    35281 agaatccacg tggataatgt ttattgtcga aatatcgatt agtcgtcgta aagctttccg
    35341 ccatcctgca cgctcctaat tctttgaccg agctagttat ggcgcggagt attagttacg
    35401 cttgacagag tgtaaaacaa aacatttaaa tcataacgac aaataatttt gcggagagca
    35461 ctgtggatac ggaattgtta aaaactttcc tggaagttag ccgaacgcgt cactttggtc
    35521 gagcggctga atcgctctat ctgacccagt cagcagtgag ctttcgaatc agacaactgg
    35581 aaaatcaact gggtgtgaac cttttcaccc gccacagaaa caatatccgt ttaaccgctg
    35641 ccggtgaaaa actactgcct tatgcagaaa cgctcatgag cacctggcag gccgcccgca
    35701 aggaggtggc gcatacctca cgacataacg agttttctat cggtgccagc gcctcgttgt
    35761 gggaatgtat gcttaatcag tggttgggac gcttgtatca aaatcaggat gcccatacag
    35821 gcttacagtt cgaagcgcga attgcccaac ggcagtctct ggtaaaacag ctgcatgaac
    35881 gccagcttga tcttcttatt accactgaag cgcccaaaat ggacgaattt agtagtcagt
    35941 tgctggggta tttcacttta gcgctttata ccagtgcccc ttctaaacta aagggagatc
    36001 ttaactatct gcgactggag tgggggccag attttcaaca gcatgaggca ggtttgatcg
    36061 gtgctgacga agttcccatt ctgacaacca gttctgctga actggcacag caacagattg
    36121 cgatgcttaa tggttgcacc tggctacccg tcagctgggc gcgtaaaaaa ggcggcctgc
    36181 ataccgttgt cgatagcaca acactttcac ggccgcttta tgccatatgg ctgcaaaata
    36241 gcgataaaaa tgcgttgatt cgcgatcttt tgaaaattaa cgtgctggat gaagtgtatt
    36301 aatctgaatg gctggcaagg atgccggtag aaggatttac ttcggagagg attatttcag
    36361 ataaaaaaaa tccttagctt tcgctaagga tgatttctgg caggggcgga gagactcgaa
    36421 ctcccaacac ccggttttgg agaccggtgc tctaccaatt gaactacgcc cctaattagg
    36481 gtggcggaac ggacgggact cgaacccgcg accccctgcg tgacaggcag gtattctaac
    36541 cgactgaact accgctccac cgaattcttt tacaaccacc ggttttacga ccggctt
//
LOCUS       DAJTRD010000031        35428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTRD010000031 DAJTRD010000000
VERSION     DAJTRD010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.031"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..725)
                     /gene="deoR"
                     /locus_tag="OO315_001738"
     CDS             complement(<1..725)
                     /gene="deoR"
                     /locus_tag="OO315_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5890679.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVK"
     gene            complement(783..1379)
                     /gene="ybjG"
                     /locus_tag="OO315_001739"
     CDS             complement(783..1379)
                     /gene="ybjG"
                     /locus_tag="OO315_001739"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5890680.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1664..2885
                     /gene="mdfA"
                     /locus_tag="OO315_001740"
                     /pseudo
     CDS             1664..2885
                     /gene="mdfA"
                     /locus_tag="OO315_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(2926..3210)
                     /locus_tag="OO315_001741"
     CDS             complement(2926..3210)
                     /locus_tag="OO315_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890681.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3296..4111)
                     /gene="ybjI"
                     /locus_tag="OO315_001742"
     CDS             complement(3296..4111)
                     /gene="ybjI"
                     /locus_tag="OO315_001742"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5890682.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4111..5319)
                     /locus_tag="OO315_001743"
     CDS             complement(4111..5319)
                     /locus_tag="OO315_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5890683.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5403..5939
                     /gene="rcdA"
                     /locus_tag="OO315_001744"
     CDS             5403..5939
                     /gene="rcdA"
                     /locus_tag="OO315_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5890684.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6114..7799)
                     /locus_tag="OO315_001745"
     CDS             complement(6114..7799)
                     /locus_tag="OO315_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5890685.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8069..8446
                     /locus_tag="OO315_001746"
     CDS             8069..8446
                     /locus_tag="OO315_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5890686.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8476..8733)
                     /gene="grxA"
                     /locus_tag="OO315_001747"
     CDS             complement(8476..8733)
                     /gene="grxA"
                     /locus_tag="OO315_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5890687.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            8893..9180
                     /locus_tag="OO315_001748"
     CDS             8893..9180
                     /locus_tag="OO315_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5890688.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9164..9886
                     /gene="nfsA"
                     /locus_tag="OO315_001749"
     CDS             9164..9886
                     /gene="nfsA"
                     /locus_tag="OO315_001749"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5890689.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            9947..10849
                     /gene="rimK"
                     /locus_tag="OO315_001750"
     CDS             9947..10849
                     /gene="rimK"
                     /locus_tag="OO315_001750"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5890690.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            10937..11413
                     /locus_tag="OO315_001751"
     CDS             10937..11413
                     /locus_tag="OO315_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5890691.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11765..12877
                     /gene="potF"
                     /locus_tag="OO315_001752"
     CDS             11765..12877
                     /gene="potF"
                     /locus_tag="OO315_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5890692.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            12972..14105
                     /gene="potG"
                     /locus_tag="OO315_001753"
     CDS             12972..14105
                     /gene="potG"
                     /locus_tag="OO315_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5890693.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14115..15068
                     /gene="potH"
                     /locus_tag="OO315_001754"
     CDS             14115..15068
                     /gene="potH"
                     /locus_tag="OO315_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5890694.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15065..15910
                     /gene="potI"
                     /locus_tag="OO315_001755"
     CDS             15065..15910
                     /gene="potI"
                     /locus_tag="OO315_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5890695.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            15970..16458
                     /locus_tag="OO315_001756"
     CDS             15970..16458
                     /locus_tag="OO315_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5890696.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16499..17626
                     /gene="rlmC"
                     /locus_tag="OO315_001757"
     CDS             16499..17626
                     /gene="rlmC"
                     /locus_tag="OO315_001757"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5890697.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(17801..18532)
                     /gene="artJ"
                     /locus_tag="OO315_001758"
     CDS             complement(17801..18532)
                     /gene="artJ"
                     /locus_tag="OO315_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5890698.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18824..19492)
                     /gene="artM"
                     /locus_tag="OO315_001759"
     CDS             complement(18824..19492)
                     /gene="artM"
                     /locus_tag="OO315_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5890699.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19492..20208)
                     /gene="artQ"
                     /locus_tag="OO315_001760"
     CDS             complement(19492..20208)
                     /gene="artQ"
                     /locus_tag="OO315_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5890700.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20215..20946)
                     /gene="artJ"
                     /locus_tag="OO315_001761"
     CDS             complement(20215..20946)
                     /gene="artJ"
                     /locus_tag="OO315_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5890701.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(20964..21692)
                     /gene="artP"
                     /locus_tag="OO315_001762"
     CDS             complement(20964..21692)
                     /gene="artP"
                     /locus_tag="OO315_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5890702.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(21910..22425)
                     /locus_tag="OO315_001763"
     CDS             complement(21910..22425)
                     /locus_tag="OO315_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5890703.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23074..24843
                     /locus_tag="OO315_001764"
     CDS             23074..24843
                     /locus_tag="OO315_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5890704.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25054..25377
                     /locus_tag="OO315_001765"
     CDS             25054..25377
                     /locus_tag="OO315_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5890705.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25374..26204
                     /gene="amiD"
                     /locus_tag="OO315_001766"
     CDS             25374..26204
                     /gene="amiD"
                     /locus_tag="OO315_001766"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5890706.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26201..27214)
                     /locus_tag="OO315_001767"
     CDS             complement(26201..27214)
                     /locus_tag="OO315_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5890707.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27313..28743)
                     /locus_tag="OO315_001768"
     CDS             complement(27313..28743)
                     /locus_tag="OO315_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5890708.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(28754..29755)
                     /gene="ltaE"
                     /locus_tag="OO315_001769"
     CDS             complement(28754..29755)
                     /gene="ltaE"
                     /locus_tag="OO315_001769"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5890709.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(29792..31510)
                     /gene="poxB"
                     /locus_tag="OO315_001770"
     CDS             complement(29792..31510)
                     /gene="poxB"
                     /locus_tag="OO315_001770"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5890710.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(31643..32611)
                     /gene="hcr"
                     /locus_tag="OO315_001771"
     CDS             complement(31643..32611)
                     /gene="hcr"
                     /locus_tag="OO315_001771"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5890711.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32623..34275)
                     /gene="hcp"
                     /locus_tag="OO315_001772"
                     /gene_synonym="priS"
     CDS             complement(32623..34275)
                     /gene="hcp"
                     /locus_tag="OO315_001772"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5890712.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34419..35318)
                     /gene="lysO"
                     /locus_tag="OO315_001773"
     CDS             complement(34419..35318)
                     /gene="lysO"
                     /locus_tag="OO315_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5890713.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8827 a         8767 c         9098 g         8736 t
ORIGIN      
        1 tacttcacat actcatcttc cgggcaacaa tcactcacca caatatcaaa gcgtttcagg
       61 tcacccatgc gcgccggacg caccttgcca aatttactgt ggtcgacaac cagcacatgc
      121 ttttgcgcca tcgacatggc ccagtgtttt accggcaact cttcaagatt aaaacaggta
      181 gcgcctttac tgacatgcac gcccgccgcg gaataaaaag cgatatccgg gcaaaaatta
      241 ttcaacgttt gctgaaaatc gatgggtttg aaaatggcgt tgctggcgtg aaattcacca
      301 ccgcacagaa atgcgcggca atggggtttc tctttcagcg ccagaaaggt atttagcgaa
      361 taacaaacgg cggtaaaagg gatttcatta tcaatcgctt caataatcca cggcgtggtg
      421 gtgccacagt caaaaaagag ggtctgatcg ggttctacca gtgtcgcagc cagttttgcc
      481 gcccggcgtt tttcttccac caggcgggat ttttgatcgc ttaacaggta atggctggca
      541 ctgcgcggtt ccagaacaat atagccgccg agcaaaacga cgggcgcact gtggttgttc
      601 agatcgcgac gaatcgtcat ctccgaaacc ccaagcaggg cggcggcgtc tttaagatgt
      661 aacttatcgc tgcgttttaa ttcttgcagc agctgcccga tacgctcttc gcgacgtgtt
      721 tccataatcc ctctgaatag ttattgaagc gagccgctca atactacact ttttagcaga
      781 gatcagtcac gcacccagcc tttgcggatc ggtaatgcaa aacagacgcg ataccacgat
      841 tgcagacgtt gatagagttt atgccccatc gcttgccaga taatctgggc actaaggcag
      901 ccaatcatac ctgccagcaa tccaccgagc atatccagcg gccagtggac gccaagataa
      961 acgcgcgacc aggcaatgac gacggccagc accattaaaa gtgagccgga ccacaggcga
     1021 tgccagcata aaaatgccag tgcaaaggtg aaaatcaccg taccgtgatc gctcgggaat
     1081 gagtcatccg ccgcatgatg caggaagtta tagccgatat tttcgacaaa gggtcggtcg
     1141 tgcggaaaaa gatgtcccat cgtccaggac acaaacaggc tgaccgccag cgcgatagcg
     1201 atttttatca ccagttgccg ttgtgctgta agcccccaca accaaagtac cgcggccagc
     1261 aacggcacca cggtaatcaa atctttagca ataaaaatcg ccaacgagat catccacgga
     1321 gccgagtctg gcgtcgcgtt aataagagag aatagagaga gattcaaatt ttccagcata
     1381 acttcccgac gcaaagtgat taaaagggag ccaatacagg caagtcgttg agaataaagt
     1441 gcaggttaaa ctgggtaaag cggcatcgtc ttatttccct caagcggcct gtttacggtg
     1501 ggtgattgta acgggcatag gttaaataaa acttaaagaa agcgtagcta tactcgcaat
     1561 aatgtaagaa tgtgcttaac cgtggtttca gctacaaaat tcgctttctc gttagctgcg
     1621 cttttattaa actctgcgcg attattattg gcgaagaaat tgcatgcaaa ataaattagc
     1681 ttccggtgcc aggcttggac gtcaggcgtt acttttccct ctctgtctgg tgctttacga
     1741 attttcaacc tatatcggca acgatatgat ccaacccggt atgttggccg tggtggaaca
     1801 atatcaggcg ggcattgatt gggttcctac ttcgatgacc gcgtatctgg cgggcgggat
     1861 gtttttacaa tggctcctgg ggccgctgtc ggatcgtatt ggtcgccgtc cggtgatgct
     1921 ggcgggtgtg gtttatcgtc acctgtctgg caatattact ggcgcaaaac attgaacaat
     1981 tcaccctgtt gcgcttcttg cagggcataa gcttctgttt cattggcgct gtgggatacg
     2041 ccgcaattca ggaatccttc gaagaggcgg tttgtatcaa gatcaccgcg ctgatggcga
     2101 acgtggcgct gattgctccg ctacttggtc cgctggtggg cgcggcgtgg atccatgtgc
     2161 tgccttggga ggggatgttt gttttgtttg ccgcattggc agcgatctcc tttttcggtc
     2221 tgcaacgagc tatgcctgaa accgccatgc gtataggcga gaaactgtca ctgaaagaac
     2281 tcggtcgtga ctataagctg gtgctgaaga acggccgctt tgtggcgggg gcgctgggat
     2341 tcgttagcct gccattgctg gcgtggatcg cccagtcgcc gattatcatc attaccgggg
     2401 agcagttgag cagctatgaa tatggctttc tgcaagtgcc tattttcggg gcgttaattg
     2461 cgggtaactt gctgttagcg cgtctgacct cgcgccgcac cgtacgttcg ctgattatta
     2521 tgggcggctg gccgattatg attggtctgt tggtcgctgc tgcggcaacg gttatctcat
     2581 cgcacgcgta tttatggatg accgccgggt taagtattta tgctttcggt attggtctgg
     2641 cgaatgcggg actggtgcga ttaaccctgt ttgccagcga tatgagtaaa ggtacggttt
     2701 ctgcggcgat gggaatgctg caaatgctga tctttaccgt cggtattgaa atcagcaaac
     2761 atgcctggct gaacgggggc aacggactgt ttaatctctt caaccttgtc aacggaattt
     2821 tgtggctgtc gctgatggtt atctttttaa aagataaaca gatgggaaat tctcacgaag
     2881 ggtaaaaaaa tgcctgattg atttgtgcga tcaggcattc tcgaattaat ggtgatggtc
     2941 gtcaatctgg tgttcgataa ccatcccttc acctacgctg gcaagatggc gaacataagg
     3001 atgcgggcgg taagccggag ctggcgcagg agccacatag acggtttgcg gcacagtcgc
     3061 gacgctgacc gcttgtggaa cgctgactga atcaggaacc acaaccactt tgtatccact
     3121 cggcacatcg acggtgacac tttgcgccat cgccacacca gtaaagccca ttaaaagcgc
     3181 gcccaacagt agacaatttt tcataatcat ttcgcctgaa ataatgcagc gggttaattt
     3241 ccgcgaatta tgcagccttc attcaggcat tgtgttgccg ggcgtgctgc cgtttttatt
     3301 ggtcaaatgg cgcttcgtgt ttaagaactt tatcgatcac atccagtacg ccttcacggt
     3361 tattgtagcc tgcccggtat tttgccgctg cgacgactgc gctgccggca ttttccattg
     3421 caaaactaaa gcctgcctga cgcagcatct caatatcgtt accgccatcg ccaaagacca
     3481 ccacttcgct gtcgtctatt ccccataatt tctgcagttg gcgaaggcca ttggctttat
     3541 gtacgccggg gataatcaga tcgatgctgc cgttgccggt gtggactggc accataatat
     3601 cgccgatggc ctcatgtaat gctttttgta cttgtggaat cagttcatcg gaaagattca
     3661 ggccaaactt aaagaaaata tcttctaagt tgtcaaagtt atcgacgtat tccagacggt
     3721 gataatacat ttccgccacc gttttcatgg catcgtcata ttttttgagt gtataggcgc
     3781 tattttttcc gcaggcaata atttccactt ccgggcgcgt cagcaaatgt tccacgacag
     3841 tagcaaacgc atccttcgac agctcgccat taaaaacatc tttgccttca ctcattaccc
     3901 agccgccgtt ttcagccaca aaggcaattt cattcgcaat ttcagggaag aaagagatca
     3961 actgataata ttgattcccg ctggcgacca caaagcgaat gccttgtgct ttcatttgct
     4021 gatactgagc cataaaccgc tcacggttat aggttttttg atcgcttaag aaagtaccat
     4081 ccatgtctac cgcaattaat ttaatgctca tcaactattc tccatcgccg tctgcgtttt
     4141 ggtatcgggt ttggcgacgg ctttcgcgac aatagccgcg agaataacca gcgccagtac
     4201 aaccagcatt gcactacgta atccataatg ttcgccgaga tagcccagca gcggcggccc
     4261 gacgaggaaa gccagataac cggtcgttgc taccacactg acgcgggttg gtgcatcggg
     4321 gccggtatcg ctggcggcag aaatggtcag cgggaagccc agcgaggcac ccagtcccca
     4381 aagtacaaca gacactcctg cgacccaggc gctatcgaca aaaataatca gcccaatacc
     4441 caacgccccc attagtgcac tggcccgaac cacggcaacg cgactgtaac tgtcgatgaa
     4501 ccaaccgccg gtgaagcgtc caacggtcat ccccagggta aaaccggcat aaatcagtga
     4561 gccggaagtg gggctaaaac cgtgaccatc aaccattaat aagggtaacc agtcgttggc
     4621 agaaccttcg gcaaaggcca tcgccagcac tacaacacca atcaacagca actggatatc
     4681 gcgataaaaa ggaatgcctt tttcgccatg ctgggtgcca tcggcagcat ttttgcccgt
     4741 accgtcaggg attgcctgaa tggcgatata aataggcgcg atacctacca gcgccgccaa
     4801 taaaatgtgc accgttgccg gaacgccaaa ggccgtcagt gccatcccga caccagcgcc
     4861 tgccagcgtg cccaggctat aaaaaccgtg catcatcggc aaaaccgttt tattcatttc
     4921 tcgctcaacg gcggcacctt caacgtttat cgccacttcc gcagaaccaa aacttgcccc
     4981 aaagacgccg agaccaacgg caaagagcag gggcgatgtc agccagagtg ccagacttaa
     5041 tatcatcatc ccgatcaatg cgcaggacat cgtgaccagg atgacattac gtgtcccaaa
     5101 gcgtttcact aaccacgccg agcagagaat accgctcatc gaaccgatcg acagaccaaa
     5161 gagaacaccg cccatttcag cgatcgagac agagagaata tcgcggatag caggcgtacg
     5221 ggttgcccag gacgccatta acaggcctgg caaaaagaag aacataaaca gcgcccaggt
     5281 tcggcgtttc aatgcattac gtgaagaatt tacggtcata gatcacgtca aaataagaag
     5341 agggaagaca acattagcaa ggttgtgtac atttgtacac aattgcagac agaggaaatg
     5401 acatgcgtcg cgctaacgat ccgcaacggc gagaaaaaat tatccaggcc acgctggagg
     5461 cggtgaaact ttacggaata catgctgtta cgcaccgcaa aattgctacc cttgccgggg
     5521 taccgttggg atcgatgacc tactattttt caggaattga tgagttgttg ctggaggcgt
     5581 tcagcagttt tactgagatt atgtcccggc aatatcaggc attttttagc gatgttagtg
     5641 atgctcaagg cgcatgccag gctatcaccg atatgatcta cagctcacag gttgcaacgc
     5701 cggataatat ggagctgatg taccagctct acgcgctggc tagccgaaaa ccgctattaa
     5761 aaacggtaat gcaaaactgg atgcagcgca gtcagcaaac gctcgaacaa tggtttgaac
     5821 ccggaaccgc ccgcgcgctt gatgcgttta ttgaggggat gacgctgcat tttgtcaccg
     5881 accgtaagcc gctatcgcgc gaggagattt tgaggatggt tgagagggtt gcagggtagt
     5941 agataaattt tagataacaa aaaacccatc aaccttgaac cgaaatggcg gggttgatgg
     6001 gctccacaaa atggggacat caaagaaaag cagtggcact aattaagact gatgccctgc
     6061 ggaaaagttc tgcggttgtg caaaaaaatt tcattttcag ggcaacttca gttttatcct
     6121 aatcctggcc ataccatgac gatgattgtc cctgccagcg tcagcaggac gttggcgatt
     6181 gcgtaggtgc ccgcatagcc cagcgcaggg atgttactgc gagctgtatc actgatgatc
     6241 tccattgccg gcgcgcaggt gcgtgcgccc atcattgcgc cgaacaacag tgcgcggttc
     6301 attcgcaata cataagcacc gaacaagaaa cagataacca cgggcacaag actgacaatt
     6361 aatccggcaa tcaacatctg accgccaatc gcgcccaggc cgttattaat accgctaccg
     6421 gcgctcagac caacgcctgc cataaacacc atcaagccga actctttcac catgcttaat
     6481 gcaccttgcg gaatgtaacc gaaggtcggg tggttagcac gcataaagcc cagcataatt
     6541 ccggcgaata acaacccggc agcgttcccc atgccgaaac tgaatgtgct gaactggaag
     6601 gtgatcatcc cgatcatcag cccaataaca aagaaggcgc aaaatgccag caggtcagtg
     6661 acctggctgt gaatcgagat aaagccgatg cgatcggcga tggtttttac gcgacgggca
     6721 tcaccgctga cttgtaaaac gtcacctttg ttaagcacga cgttgtcatc tatcggcatc
     6781 tcaatctggc tacgaatgac gcggttaagg aagcaaccgt gatcggtcaa cttcagttgt
     6841 gcgagacgtt tacctacagc gttatggttt ttaacgacca cttcttcagt gacgatacgc
     6901 atgtcgagaa ggtcacgatc gaaaacttct ttaccgttac ggaagctggg atcgagtcgg
     6961 gcatgggcgt cgggatagcc taccaacgct atttcatcgc ccatttgtag cacggcatca
     7021 ccgtctggat ttgccagaat cccgttacgt cgaatacgtt caatgtagca gccggtttgt
     7081 cgataaatac ccagttcacg cagatttttg ccgtcggtcc aggccaccag ctccgggccg
     7141 acgcgatagg cgcggatcac cggtaaataa accttacggt tggcatcagt gtccaggcca
     7201 cgttcgcggg cgatttgctg ggcgctggtc tgtaagtcct gatgctgcaa tttcggcaag
     7261 taacgcgcac caacaatcaa actcaccaga ccgattaaat aggttaaggc atacccgagg
     7321 ctcagattat ccagtgccag tgagagctgc ctgctttcca tgccggaatg atgcagtgta
     7381 tcgccagcac cgaccagaac cggtgtcgac gtcatagagc ctgctaacat accggccgtc
     7441 aggccaatat cccagccaaa cagcttacct aaccctaagg cgatcaccag cgcactgcca
     7501 accatcacca gtgctaacat taggtaattt ttcccatcgc gaaaaaaaat ggaaaaaaag
     7561 ttcggtccgg cttcgacccc gacgcagaaa ataaacagca taaagccaag attaagcgca
     7621 tcggtgttaa tgctgaaatg ttgttggcct aataacagcg atacgactaa aacgccaatg
     7681 gaattaccca gttggatcga accaagtcgt aactttccga gacatagccc aagcgcgagg
     7741 accacaaata ataacagaat gtaattccca tttaacaatt cggcgacgtt tatattcacg
     7801 gaggctaact tcttgtttac tagtaagctg ttgaaagaaa tgctaattta cgataatgtg
     7861 ttttaccaga attcagggcg cagattcatt cagcgcacct aaacgatagt aaagtaacaa
     7921 tatattttac tagtgtaatc acattaggta tcaacggcta tatgaattgc gttggcctat
     7981 attagcatgg aatgcgaagc ggctttatct tactgaacgc cacactggcg aaaaatgtgt
     8041 tcgatagacg cagtgtcagg aggaacgagt gaaacataaa caacgttggg cgggggcaat
     8101 ctgctgtttt gtcctcttca ttgtggtgtg cctttttctg gcgacgcaca tgaaaggcgc
     8161 ttttcgggct gccgggcatc ctgaaatcgg cttgctgttt ttcattcttc ctggagcagt
     8221 tgccagtttc ttttcacagc gtagagaagt cctgaaacct ctatttggcg caatgctggc
     8281 ggcaccctgt tcaatgctca ttatgcggct gtttttttca ccgacgcgct cattctggca
     8341 agagctggca tggttactaa gcgcggtgtt ctggtgtgcg ctgggggcac tgtgtttctt
     8401 atttatcagt agtttgttta aaccacagca cagaaaaaat cagtaaagcc ctcaacgcga
     8461 gggcttgtca gacgatcagg cgtccagatt ttctttcacc catgcagcaa aatcggtata
     8521 gccgccgata tgttgctgat cgacaaaaat ctgcggcacg gtttctacgg gtttacctgc
     8581 cttttgttgt agatcttctt tagtgatccc ttccgcacga atatctacat actgatactg
     8641 aaaatcatcg cgttcattgc tcaatttctc agccagatct tttgcacgca cacagtaagg
     8701 gcaacccgaa cgaccaaaaa taacggtttg cattatttct ctcctcatag atttatgcct
     8761 gtaatgatca cgctaaaatg tattcgctga aagtaggttt aacctgttgc attaattgct
     8821 aaaagctata actgttaaac acaatacagt gaaaagtttt agactgaagg ctcactttgc
     8881 agagggaagc gtatgcgcgc gatcggtaaa ttgcctaaag gcgtgttgat actggaattt
     8941 atcggaatga tgctactggc ggtggcgctg ctgtcggtaa gcgactccct gtcgctgcct
     9001 gagccatttt ctcggccaga agtgcagatt ctgatgattt ttctcggtgt tttgctcatg
     9061 cttcccgctg cggtggtggt tattcttcag gtggcaaaac gtcttgcccc acagctgatg
     9121 aactgtccac cgcaatattc acgttcagaa agagaaaaag ataatgacgc caaccattga
     9181 acttatttgt ggccatcgct ccattcgcca tttcactgat gaacccattt ccgaagcgca
     9241 gtgtgaggcg attattaaca gcgcccgtgc gacgtccagt tccagttttt tgcagtgtag
     9301 tagcattatt cgcattaccg acaaagcatt acgtgaagaa ctggtgacgc tgaccggcgg
     9361 gcaaaaacac gtagcgcaag cggcggagtt ctgggtgttc tgtgccgact ttaaccgcca
     9421 tttacagatc tgtccggatg ctcagctcgg cctggcggaa caactgttgc tcggtgtcgt
     9481 tgatacggca atgatggcgc agaatgcatt aaccgcagcg gaatcgctgg gattgggcgg
     9541 ggtatatatc ggcggcctgc gcaataatat tgaagcggtg acgaaactgc tgaaattacc
     9601 gcagcatgtt ctgccgctgt ttgggctgtg ccttggctgg cctgcggata atccggatct
     9661 caagccgcgt ttaccggcct ccattttggt gcatgaaaac agctatcaac cgctggataa
     9721 agacgcactg gcgcagtatg acgagcaact ggcggaatat tacctcaccc gtggcagcaa
     9781 taatcgctgg gatacctgga gcgatcatat ccgccgaaca atcattaaag aaagccgccc
     9841 atttattctg gattatttgc acaaacaggg ttgggcgacg cgctaaaacc gccacgtcga
     9901 tgtatgatac gcgggctttt gaccaggtct gacagagagg tgcagggtga aaattgccat
     9961 attgtcccgg gatggaacgc tctattcgtg taagcggctg cgtgaagccg ctatacagcg
    10021 cggtcacctg gttgaaattc ttgatccgct ttcttgctac atgaacataa atcctgcggc
    10081 gtcttctatt tactacaaag gccgcaagtt accccatttt gacgcagtga tcccgcgtat
    10141 tggcaccgcc attacctttt atgggacggc ggcactgcgc cagttcgaga tgctggggag
    10201 ctatccgctc aatgagtcgg tcgccattgc ccgggcgcgt gacaaattgc gttccatgca
    10261 actgctggcg cgtcagggca tcgacctgcc tgtcacgggc attgcgcatt cgccggatga
    10321 taccagcgat ttaatcgaca tggtcggtgg tgcgccgctg gtggtcaagt tggttgaagg
    10381 cacgcaggga attggcgtcg tgctggcgga gactcgtcag gcggcggaaa gcgtgattga
    10441 cgctttccgc ggtctgaacg cgcatattct ggtgcaggaa tatatcaaag aggcgcaagg
    10501 gtgcgatatc cgctgtctgg ttgttggtga tgaagtggtc gctgcgattg aacggcgggc
    10561 gaaagagggc tattttcgtt ccaatttgca tcgtggcggc gcggcaagcg tcgccagtat
    10621 cacaccacag gagcgtgaaa tcgcgataaa agccgcgcga acgatggcgc tggacgttgc
    10681 tggtgtggat attctgcgtg ctaatcgcgg gccgttggtg atggaagtga atgcgtcgcc
    10741 ggggctggaa ggaatagaaa aaaccaccgg tatcgatatc gcgggtaaaa tgatccgctg
    10801 gatcgaacgc cacgctacga cagaatattg cctgaaaacg ggtggttagt cgcaatcaca
    10861 ttactgatca tggttttgcc tgcgcttttt gcgtaagctg tgccggtctt tttatcgaaa
    10921 gaggttgtac aaaattatga catcgctggt cgttcctggt ctggatacgc tgcgtcaatg
    10981 gctcgatgac ctggggatga gtttttttga atgtgataac tgtcaggctc tgcatctgcc
    11041 ccatatgcag aatttcgacg gtgtctttga tgccaaaatc gatctgatcg ataacacgat
    11101 cctgttttct gccatggcgg aagtccgacc ttcagccgta ttgccgctgg cggcggattt
    11161 atctgccatc aatgccagtt cgctgaccgt gaaagcattt cttgatatgc aggatgataa
    11221 tctgccaaag ctggtggttt gccagtcttt atccgttatg cagggcgtaa cctatgagca
    11281 gtttgcatgg ttcgtgcgtc agagcgaaga gcagatttcg atggtcattc ttgaagctaa
    11341 tgcccatcaa ctgctgttac cgactgacga tgaagggcaa aacaacgtta ccgaaaacta
    11401 ttttctccac tgataattcc tttcgagcac gcagtcgctg ccgcagtgac tgcgcgctgc
    11461 aaaattatct gctgttttta accttttctt aaagattatt tcacttctct tgtgtcgatt
    11521 tggctttatc acatagagca aatacgcata aaaatttgtt aaataccgtt ttttaatccg
    11581 agctatagtc tcaaaccctg gctaaagtta ttcttgcgat gcttctatat agcgagcagt
    11641 gctggccggg agagagttct cttttcttac accgcgccga taaaaaaata tgcacgttta
    11701 ttgcatatct ttcagtgtga caacttttgt tcgtttgtta acgaactttc agaaggaaag
    11761 agatgtgacc gccttaaata aaaaatggct atcgggtctg gttgcgggtg ctctgatggc
    11821 cgtctctgtc ggcacgctcg cggctgaaca aaaaacactc cacatttata actggtctga
    11881 ttatatcgcc cctgacacgg tggccaattt tgaaaaagaa accggtatta aagtcgtcta
    11941 cgatgttttc gactctaacg aagtactgga aggcaaatta atggcgggta gcaccggctt
    12001 tgacttggtg gttccatctg ccagctttct agaacgccag ttgactgcgg gagttttcca
    12061 gccgctggac aaaagcaaat tgccggagtg gaagaatctc gacccggagc tgctgaagct
    12121 ggtcgccaaa cacgatcccg acaataaatt tgctatgccc tatatgtggg cgacgaccgg
    12181 gattggctat aacgttgata aagttaaagc tgtgctgggc gaaaacgcgc ccgtcgatag
    12241 ctgggacttg atcctcaaac ctgaaaatct ggaaaaactg aaaagctgcg gtgtctcttt
    12301 cctggatgcg ccagaagaag tttttgctac cgtgttgaat tatctcggca aagatcccaa
    12361 cagcactaaa gcggatgatt acaccggacc ggcaaccgat ctgctgttaa agctgcgccc
    12421 gaacattcgt tatttccatt catctcaata cattaacgac ctggcaaacg gcgatatttg
    12481 cgtcgctatc ggctgggcag gtgatgtctg gcaggcgtca aaccgcgcga aggaagcgaa
    12541 gaatggagtg aatgtctcgt tctcgattcc aaaagaaggg gcgatggcgt tctttgatgt
    12601 attcgccatg ccagcggatg ccaaaaacaa agacgaagcc tatcagttcc tgaattacct
    12661 gctgcgcccg gatgtagtag cgcatatttc cgaccatgtg ttctatgtca acgccaataa
    12721 agcagccacg ccgctggtga gtgcggaagt ccgtgagaac ccgggtattt atccgcctgc
    12781 ggatgttcgt gcgaagctgt tcactctgaa agtgcaggac ccgaaaatcg accgtgtgcg
    12841 cacccgcgcg tggaccaaag tgaagagcgg aaaataatcc gcagccttag atgccgggcg
    12901 ggcgcaccac acccgccggc aattcgcacc atcatggtgc gcttgcacac attcaatgcc
    12961 ggagagcagc cgtgaatgac gctatccctc gcccgcaggc gaaaacccgt aaggcgctga
    13021 cgccgctatt agaaatccgc aacctcacca aatcctacga tggtcaacat gcggtggatg
    13081 atgtcagcct gaccatctac aaaggtgaaa tcttcgcgct gctgggcgca tccggctgtg
    13141 gcaagtccac gctgctgcgt atgctggcag gtttcgaaca accttctgcc ggacagataa
    13201 tgcttgatgg cgtcgatttg tcacaggttc cgccttacct gcgccccatc aatatgatgt
    13261 ttcagtctta cgcgctgttt ccgcatatga ccgtggaaca gaacatcgct tttggcctga
    13321 aacaggacaa actaccgaaa gcggaaattt ccagtcgggt caatgagatg ctcggcctgg
    13381 tgcacatgca ggagttcgcc aaacgtaaac cgcatcagct ttctggtggt cagcgacagc
    13441 gtgtggccct ggcccgaagc cttgccaagc gcccgaaact attactgctc gacgagccga
    13501 tgggcgcgct ggataaaaag ctgcgtgacc ggatgcagct tgaagtggtg gatattctgg
    13561 agcgcgtcgg tgtgacttgt gtgatggtca cccacgatca ggaagaggcg atgaccatgg
    13621 cggggcgcat cgccattatg aatcgtggga aatttgtcca gattggcgaa ccggaagaga
    13681 tctacgagca tccgactacc cgctatagcg ccgaatttat tggctcggta aacgtctttg
    13741 aaggcgtact caaagagcgt caggaagatg gcttggtgct tgattcgccg gggctggtgc
    13801 atccactgaa agtcgacgcg gatgcctcgg tggtcgataa cgtgccggta catgtggcgc
    13861 tgcgcccgga aaaaatcatg ctttgcgaag agccgcccgc caatggttgt aacttcgcgg
    13921 tgggggaggt gatacacatt gcctatctcg gcgatctttc ggtgtatcac gttcgtctga
    13981 aaagtgggca gatgattagt gcccagctac aaaacgccca tcgtcatcgt aaagggttac
    14041 cgacctgggg cgacgaagtg cgtttgtgct gggaagtgga cagctgtgtg gtgctgacgg
    14101 tttaaggagc aaagatgaat acacttgaac ctgctgccca gtcgaaaccg ccgggcggat
    14161 ttaagctgtg gttgtcgcag ctgcaaatga agcatgggcg caaactggtc attgcgttgc
    14221 catatatctg gttgatcttg ctgtttctgc tgccatttct gattgtcttt aaaataagcc
    14281 tggcggagat ggcgcgcgct attccacctt ataccgaact gatggagtgg gctgacgggc
    14341 aactgtccat cactcttaat ctcggtaatt ttctgcaact gaccgacgat ccgctctatt
    14401 tcgatgctta tctccagtcg ctacaggtgg ctgcgatttc gacattttgc tgtttactga
    14461 tcggctatcc gctggcgtgg gcggtggcgc acagtaagcc ttcgacccgt aatattttat
    14521 tactactggt gatcctgccg tcgtggacct cgtttctgat ccgcgtttat gcttggatgg
    14581 gaatattaaa aaacaacggt gtgctgaata attttctgct gtggctgggg gttatcgatc
    14641 aaccgctgac cattcttcat accaatctgg cagtttatat tggcattgtt tacgcctacg
    14701 tgccgtttat ggtgctgccg atttataccg cgttgattcg tattgattat tcgctggtgg
    14761 aagcagcgct ggatctcggt gcacgaccgc tgaaaacgtt ctttactgtg atcgtgccgc
    14821 tgactaaagg tgggattatt gccggatcga tgctggtgtt tatcccggct gtgggcgagt
    14881 ttgtgatccc ggaactgctc ggtggcccgg acagcatcat gatcgggcgc gtgctatggc
    14941 aagagttttt caataaccgc gactggccgg tggcctcggc ggtagcgatc atcatgttgc
    15001 tgctgctaat tgtgccgata atgtggttcc acaaacacca gcaaaaaagc gtgggagaac
    15061 acggatgaat aatttaccgg tagttcgttc gccctggcgg attgtgattt tgctgctggg
    15121 ctttaccttt ctctacgcgc caatgctgat gctggttatc tattcgttta acagctcgaa
    15181 gctggtgacg gtgtggggcg gctggtcaac gcgctggtat ggtgagttat tgcgcgatga
    15241 cgcgatgatg agtgcggttg gttcaagcct gacaattgct gcctgtgcgg caacggcggc
    15301 ggcgatcctc gggactattg cggcggtggt gctggtgcgc tttggcaggt ttcgcggatc
    15361 aaatggcttt gcctttatga tcaccgcgcc gctagtaatg ccagatgtca tcacgggctt
    15421 gtcgctgttg ttattattcg tcgcgcttgc tcatgccatt ggctggcctg cggaccgcgg
    15481 tatgcttacc atctggctgg cgcatgtcac tttctgtacg gcttatgtgg cggtcgttat
    15541 ttcgtcgcgt ctgcgggaac tggatcgctc gatagaagaa gccgcgatgg atctcggtgc
    15601 gacgccgctg aaagtgtttt tcgtcattac gctaccgatg atcatgcccg cgatcatttc
    15661 tggctggtta ctggctttta ctttgtcgct tgatgatctg gtgatcgcca gctttgtttc
    15721 cgggccggga gccaccacgt taccgatgct ggtcttttcc agcgtgcgga tgggggtgaa
    15781 tccggaaatc aacgccctgg caacattgat cctcggcgcg gtcggaattg tcggatttat
    15841 cgcctggtat ctgatggcgc gcgcagaaaa acagcggata cgcgacatcc aacgtgcaag
    15901 acgtggctga agacactaaa atttgccaac ctggctacat aatgccgcgc atgtcgcggc
    15961 attgttttca tggaagacga aacgttggga ttttttaaga aaacatcttc atctcatgct
    16021 cgcctgaatg tgcctgcgct ggtgcaggtg gcggcgctcg ccattattat gatccgtggc
    16081 ctcgacgtgc tgatgatttt caatacgctg ggcgtgcgcg gtattggcga gttcattcat
    16141 cgcagcgtac aaacctggag tttaacgctg gtctttttaa gcagtctggt gctggttttc
    16201 attgagatct ggtgtgcgtt ttcactggtg aaagggcgtc gctgggcgcg ctggctatat
    16261 ctgctgacac aaatcaccgc cgcaagttac ttgtgggcgg cttcgctggg gtacggttat
    16321 ccggagctgt tcagcattcc cggtgaatca aaacgtgaaa tcttccatag cctgatgctg
    16381 cagaagctgc cggatatgct catcctgatg ctgctgttcg ttccctcgac cagtcggcgg
    16441 ttcttccagt tgcaataatg tgtataatcg tcgcccctga tgatgtgaag gtcaatgtat
    16501 gcagtgcgca ctttacgacg cgggtcgctg tcgttcctgt cagtggataa cgcagccgat
    16561 tccagagcaa ctctccgcta aaaccgccga tcttaaaaat ctgctcgccg attttccggt
    16621 tgaggaatgg tgcgcgccgg tgtcaggccc ggaacaaggg tttcgtaata aagccaaaat
    16681 ggtggtgagt ggtagcgttg aaaaaacact gctcggtatg ctgcatcgcg atggcacgcc
    16741 ggaagacctt tgtgactgcc cgctttatcc agcctcattt gcgcccgttt ttgcggcgct
    16801 aaaaccgttt atcgcccgag cggggttaac gccctacaac gtggcgcgta agcgtggcga
    16861 actgaaatac attctgctga ctgaaagcca gagtgatggc ggcatgatgc tgcgctttgt
    16921 actgcgttct gataccaagc tggcgcaact gcgtaaggcg ctgccgtggt tacacgaaca
    16981 actaccgcag ctgaaagtta ttaccgtcaa tattcagccg gtacatatgg cgattatgga
    17041 aggggagacg gagatctacc tgaccgaaca acaggcactg gcggagcgtt ttaatgacgt
    17101 accgctgtgg atccgtccgc aaagtttctt ccagactaat ccggcggtcg ccagccagtt
    17161 gtacgccacc gcgcgcgact gggtacgaca gctgccggtt aaacatatgt gggatctgtt
    17221 ctgcggtgtg gggggctttg gtttacactg cgcgacgcct gacatgcagt taaccgggat
    17281 cgaaattgcg ccagaggcca ttgcctgtgc aaagcagtca gccgctgaac tgggcttaac
    17341 tcgtttacaa tttcaggcgc tggactccac tcagtttgcc actgctcagg gggaggtgcc
    17401 ggagctggtg ctggttaacc cgccgcgccg cggcattggt aaaccgctgt gtgattatct
    17461 ttcaacgatg gcaccgcgtt ttattatcta ctccagctgt aacgcccaaa ccatggcgaa
    17521 agatatccgc gaactgccag gttaccgcat cgaacgggta cagctttttg atatgttccc
    17581 gcacaccgcg cactatgaag tgttgacgct gctggtgaag caataaaaaa gccacaggtg
    17641 cggcttcaga ttgctgatca ggtgcgcttt gttcatgccg gatgcggcgt gaacgcctta
    17701 tccggcctac aaaatcatgc taaatcaata cattgcagag ttgatgtggg ccggataagc
    17761 gcagcgcatc aggcaatctt acttttgtca tcagtctcaa ttactgcgga aaccactggt
    17821 cattgatttt ctgatatgta ccgtcagctt taattgctgc cagcgcgtta ttcagttttt
    17881 ccagcagagc tttgttatcc ggacgtacag cgatacccag gccggtgccg aagtattgcg
    17941 gatcggtcac tttttcagtc gcaacgccca gttgcggatt ggttttcagc cattcgttta
    18001 ccaccgcggt gtcaccaaat accccatcaa tacgaccatt tttcagatcg ataaaggcat
    18061 tctgataact gtcataggag acagttttca cttccgggtg ctgatcctga atatatttct
    18121 ggtgcgtcgt accgttttcc atcccaatgc gtttgccttt caggtcggca aacgttttgt
    18181 aggtatcttt tttggaaatc acgacggctg agttttcata gtagggtgtg gtaaacgaaa
    18241 cctgcttgct acgctccggg gtgatatcca taccggagat tacggcgtca tattttctga
    18301 atttcagtga cgggatcagg ctgtcgaacg cgtgattagt aaaagtacat tctgcctgca
    18361 tttgtttgca caaggctttt gccagatcga tatcaaagcc gacaatctca ttattagcac
    18421 ctatagattc aaagggtgga taggtggctg aaacgccaaa attgattttc tctgcggcag
    18481 aagcaccgaa agtaaaggaa gcaagtaaag cggcaagaac taactttttc atgatggaac
    18541 tcccgtctgt caatcttatg atttttggcc gtgtctgcgg catgggataa caatgccatc
    18601 aagtgaattt atatgcaata aacatgatta aataatttaa atgaaataaa aaaagacgga
    18661 taacttagtg agttgtccgt cttcattata agaatttatg cactatgtag gccggataag
    18721 gcgtccacgc cgcatccggc acaggcaccg tgctgatgtc tgatgcgacg ctggcgcgtc
    18781 ttatcagacc tacaaacccc cccggcgaat gtacgcagct acattaattc cgccgttcga
    18841 atgccagtgc tttgcgctcg atcagacgca tcatcagcgt cagcaggccg ttgacgacca
    18901 ggtaaataat ccctgccgca ccgaacacca ttacatcgta ggtgcgtccg tacaacaact
    18961 ggctgtatcc catcacttcc atcagcgtaa tggtgtaagc cagagaggta cttttgaaca
    19021 ccagcaccac ttcgttggaa taagaagaga gcgagcgttt aaaggcatac ggcagcagga
    19081 tcgccagcgt atcttttttg ctcattccca gggcgctaca ggactgccac tgaccttctg
    19141 ggatcgcacg aattgcaccg taaaacagct gcgtggtata cgccgcactg ttcagcgata
    19201 acgcaatcag cgcacataac cacggttctg acaacaaatg ccacagtgcc ggatactcct
    19261 gcaacgtcgg aaactggccc ggcccgtaat aaatcaggaa gatctgcacc agcagcggcg
    19321 taccggtaaa cagcgtgata taaccccgca ccagccacac cagcaccggc gttttcagcg
    19381 tcaggatgat ggtaaaaatc aatgccagaa tcagtgccac aatcagcgag gcaacggtta
    19441 gcgtcaggct ggtgtgcagc cctttcatca gctcgggtaa atactcaaac attagctggg
    19501 cctccgctca aaacgtgtcg cgcgcaggtc aatgcgtttg agaatgtact gactgagcag
    19561 ggtgatcacc aggtaaatcg ccgccgccac aatgtaccag gtaaatggtt cctgggtacg
    19621 agtagcaatg cttttggttt gtaacattaa atcattcaca ctaatcaaac tgaccagcgc
    19681 ggtatctttc agcagcacca gccactggtt accgaggcca ggcagcgcat gacgccacat
    19741 ctgcggcatc accagacgga aaaagatagc cgatttcgac agccccagcg cctgaccaga
    19801 ttcccactgg cccaccggta cagctttcaa cgcgccgcgc agcgtttgcg aggcataggc
    19861 ggcatacagc agtgacagag cgatgacacc acaaaggaac ggactcacgt cgaagttctc
    19921 aatgtccatc tgcaccggga tctgcacgaa cccaagattg atagtgaagc catccgaaag
    19981 cgtcagcagt aactgcgagg agccaaaata gataaacagc actaccagaa tttctggcag
    20041 gccacgcaga atggttacca gcgctgaacc tgcccacgcg acaggacgcc atttcgctga
    20101 ctcccatacc gcaaagaaca tcgccagcgc cagcccgaca atcaatgcac aaacggcaag
    20161 gccgacggtc atcccggcgg cgcttgctaa aggaaaaaat tcattcatca ggaattactt
    20221 ctggaaccat ttgttgtaga tggtttcgta agtgccatct ttcttcactt tttccagcgc
    20281 agtgttgagt ttctgctgca gttcagtgtt tccctgacgt accgcgatgc cgaggccagt
    20341 accgaaataa tctttatcgg tcactttatc gcccactgcc gccagttttg ggttatcttt
    20401 cagccactca gtgaccactg cggtgtcacc aaatacgctg tcgatacgcc cgttttgcag
    20461 atccagtttt gcgttctggt agctgtcata cggaacggtg gtgatttccg ggtgcttatc
    20521 cataatgaac ttctggtgtg tcgtcccgtt ctgaacgccg acttttttgc ctttcagctg
    20581 atcaacactg gtgtatttgc cttgctgacc cacaaacagg gcagagttgt catagtacgg
    20641 ggtagtaaac agtacctgct tttcacgctc cggagtgata tccatgcccg ccatcactgc
    20701 ttctacgcga cggaatttca ggcttgggat caggctgtca aacgcctggt tagagaaagt
    20761 acaggttgca tcaatctctt tacacagtgc ctgcgccagg tcgacgtcaa aaccaacgat
    20821 ctggttgttt gcatcaatcg attcaaacgg aggatagggg gcttcggtag caaaacgaat
    20881 ggtttcggca gctgtggcgg aaagactaaa acctgcaatt aacgcggcaa tcagaacttt
    20941 tttcattgtt gttatcccga atcttagtga gagagatagt ttttaaatgc ttcggtttgc
    21001 ggctcggtaa agcagctcgc gtcgccttgt tctacgatat gaccattttc catatacacc
    21061 actcggctgg cggttttacg cgccacttca acttcgtggg tgacgatcac ctgggtaata
    21121 ttcgtttctg ccagctcacg aatgatgctg acgatttgtg ccgtaatttc cgggtccagt
    21181 gcggcggtcg gttcatcgaa cagcagtacc tgcggttcca tcatcaacgc acgggcaata
    21241 gcaacacgct gctgctgacc accagaaaga tgcagcgggt aacgatcgct ataaggtttg
    21301 agacgcagac gttccagcag tttttctgca cgggccagcg cctgatcttt actcaacccc
    21361 agtacacggc agggcgcttc aatcaggttt tgctgcacgg tcagatgcgg ccacaggttg
    21421 tattgctgaa acaccatgcc aacgttacga cgcaaatcgc gaatcgcttt gtcagagggt
    21481 gttttggtga aatcgaaatg gttgcctgca atgttgagcg taccggagcg cggcatctca
    21541 agcagattga gtacacgcag cagcgagctt ttacccgcgc cgctggggcc aagtaacacc
    21601 agcgtttcgc cctgtgggca atccagcgtg atatcgaaca gcgcctgatg cgcgccgtag
    21661 aagcaattaa tgccgtttaa ttgaatactc attgacactc gtatactggc agtctgatag
    21721 ctattgaggt cgaagatagt acctttgaca gaataattat gcaatattta tgctttaaaa
    21781 gttaaaagca aagcgcatta ttcaataaac atagcacaaa ataacggggg cggtggtcgg
    21841 tgagcataaa tgtcggcatt cctcacgaaa tgccggataa tttacggggt ttattggttg
    21901 atcaaggcgt tagcgattct cgatggactg acggagcgta cccgccgtgg catgaacgct
    21961 accgcctaag tagcgcacat cgtcgatgac ccaacactgg ccttcctgaa tcattaacac
    22021 ttcatcctgc cagccctggt caccctgttt gagatccacc cgcaatggaa tgttacgggc
    22081 atcacgatta gggatagtcg atgcactggc aacgtgggcg ctgtctggca aggtggttcg
    22141 actggagaac ggatcgctgc ttaacaattc acgatgacta ttatcgcggc tggcatcgct
    22201 aagttgggtt gccagtttgt cactcaaata ggggcgcagg gcggtaatgt cattgctgcg
    22261 atgttgaatg cggtagtcgt agaattgttg agccacactg tcaggccctc cctggacaca
    22321 ggaaccggtg cgggggccgt tatctttata agctggcgtg acggtggtac aggcactcag
    22381 cagcagtgca catggaataa gtagagatag tgaacggtag cgcataatca tttccttatt
    22441 agcttgctgt ctgcgtcaca ctacgaccaa acgctatcag attatttaat acattgtttt
    22501 taatacaatt ctgaaacaat ctgaacacct gattgggttt gagtccattg ctggcgaccc
    22561 actcaaggtt gctcgtagct gacttactta aattacccaa acaatagaca gtgtaaacca
    22621 tttgtgaggt tgagcgtgtt atatggctga aatgatcatt taaggtaaat atgatagttc
    22681 tgaaatggtg gtataaatcc tcctgtagag gttaagagca tctgcaagaa gccagtttca
    22741 catttataac gcgtgataaa tgaatgcggt tattgtctaa tcattacaaa atttttcaca
    22801 tgcgataaga gatcctgcga ttaagatgat tgccaaaatt acgttgtatt tttgaaaatg
    22861 agcgaaattt gatagtccca gctttgactc tcgataatat tatttatgct acaattgtgg
    22921 gtaaagaatt taagctaatg aatttgagtg aagaataaat taaattgctt cagtatttcg
    22981 ttaattaata taaatacaaa aggcgttgtg cttttataat tataaagcgc gaggagttcg
    23041 gtggattatc gtttttcttg ggtgattttt aatatgataa acaatatatc tgatcaagca
    23101 tcatcatttc caggcacaca actaaatcaa agtgataatt tcctggattc actgagggaa
    23161 tttttcgcga tattaaatcc cagtcgtaag ggtgagttgt ctacatggga taccatctac
    23221 ttgcatctga tattagcaat aaatgctgac agtgatctca taaaaaatga tgtgttgctc
    23281 gcggaaaata ttccatcagc gaattaccaa tttaatacat tttttagcaa tactttcgag
    23341 attgatgtta aaaaatatct gggtaaatca gaagataacg aagttgaaat aaaagcgggt
    23401 aatgaaagaa tctctattgg aatacgtaat gtatcgaatg gaaaacttga aagacaacag
    23461 tttcttttcc cactggacta tgagaataaa ttacaagaac aattagataa gtattttacg
    23521 attgaatcgc acccattatt atacaggtat accattggta gtaagattgc gaatgtgatt
    23581 tttgaaaagt tatacagtcg tattgacttt aacaaagaac aatatatatc ctttataaag
    23641 gacgcattta ttcattttta tgattatagc aggcgatatg ctattagtga gaatatcgat
    23701 aaagacgctg taacaaacaa tatcgcattg atgagcactt tttatgacag tgataatacg
    23761 tcaggtgagg ttttaaataa tgattttaca gaagaagagt catttgaaac tgcgttagat
    23821 gtagagcatg caatagtgct tggatttgct gatgataatt ttgaaacaaa acctgttcat
    23881 tatcaggatt tattaacacg atttagtgcc tttcaagata ccgtgtttaa tctgtttcct
    23941 gaaatgcact cttcgcatta ccacgatatt tgtagcgtaa gtgtggatat gacaaaggga
    24001 acgcaatgca tgattcactt gatggtgaat gaagaagttt tcatgtctct gcctgttcca
    24061 gttgccacga tggtacgtga agatgcaagt aatttagtca atcttaaaac actactcaat
    24121 gatggttgtt tcattaaata tagtcatttt aatgacgtgg cattaataaa gcaaaacata
    24181 agtaatctct atttatcaca cactgttgtg aatgaaagca tattgaaaaa atgttgtttt
    24241 gaaaacggca gtttaggtga tgttaaaata acaaatagca atgtaataaa cagtgcattc
    24301 aaaaatattt cgtttagatc tgttaaaatt aataacgtaa atactcattc tttaaaattt
    24361 attaactgca attttttcaa tgttgatatg ataagagtta atctcagcaa atgtcttttt
    24421 catgaatgta gtatgcatgg tgtcaagata aaaccctggt tgcctgtaaa atggacaaaa
    24481 gaattaataa gtgattattt gtatggctgt ttactatctc tgtactctat ctgtgcaagg
    24541 gatatatata atatgaacgc cgggaataac gtgaaggttg ctgcggatgc tttccttgaa
    24601 attattttct ctcttaaaaa taaatattgt attaaactat taagcgctca ggatcgggcg
    24661 tttatatacg agtttgcaag aatgatcttc gcttatatta atgacaaaag cattgagatc
    24721 ttattacttt cttgttttgc ggcggctgac caaaaagcaa tccagcggta tagacctcag
    24781 tcacaagatg gggaagactt taggagtcat ttacaatata agttgcctct ttccgctcat
    24841 taatgttgtt ggtgcaacat aataagacag gtaagcggta ccctataatg aaaccaatat
    24901 cgggttatgc aacggataat cgtaaagact taagtgggtg tttttgacac aatgagatga
    24961 tttcctaaac tgatcaaaac cttcttccca acatggcata tcagtccgat aatgctttag
    25021 agatcgaagg cttaacaaac aaggagatcg atcatgcaat tttctacaac cccaactctg
    25081 gaaggcctga ccatcgttga atattgcggt gtggtgaccg gcgaagcgat tttaggtgcc
    25141 aatattttcc gtgatttctt tgccggtatc cgcgatatcg ttggcggacg ttccggtgcg
    25201 tatgaaaaag aactgcgtaa agcacgggag atcgcctttg aggaattagg ctcccaggcg
    25261 cgggcgctgg gggccgatgc cgtagttggt attgatatcg actacgaaac ggtcgggcaa
    25321 aacggcagta tgctgatggt tagcgtcagc ggtacggcgg tgaaaacgcg tcgatgagaa
    25381 gagtcttctg gctggtcgct gccgctctgt tactggcagg gtgtgcaggc gaaaaaggca
    25441 ttgtcgagaa agagggatat cagcttgata cccgacacca ggcgcaggcg gcgtatccgc
    25501 gcattaaagt gctggtgatc cactacaccg cagatgattt tgatagctcg ctggcgacac
    25561 tgactgataa gcaggtcagc tcgcattatc tgatccctgc ggtgccaccg cgatacaacg
    25621 gtaaaccgcg catctggcaa ctggtgccgg aacaagaact ggcctggcat gcggggatta
    25681 gcgcctggcg cggggcaacg cgccttaacg acacctctat tggcattgag ctggaaaacc
    25741 gtggctggca aaaatcatcc ggagtgaaat attttgcccc gtttgaacag gcacagattc
    25801 aggcgcttat tccgctggcg aaagatatta ttgcccgtta tcacatcaag ccggaaaacg
    25861 tagtggcaca tgcggatatc gcaccgcagc gcaaagacga tccggggcca ttatttccct
    25921 ggcagcaact ggcgcagcag gggattggtg cctggccgga tgcgcagcgg gtgaactttt
    25981 accttgccgg gcgcgcgccg cacacaccgg tagataccgc gtcattgctg gagcttttgg
    26041 cgcgctacgg ttatgacgta aaacctgata tgacaccgcg cgagcagcgg cgcgtgatta
    26101 tggcattcca gatgcatttc cgcccgacgt tatataacgg cgaagcggat gcagaaactc
    26161 aggcgattgc cgaagcattg ctggagaaat acgggcagga ttagcgcggc agttttccgt
    26221 ggtcgcgtag ccaggcggca gttttctcga tgccttcatc cagggtgatg accggctgat
    26281 aacctaactc ttcctgcgca cgcgtaatat ccagcgtaaa gtcaaaattc aacttggagg
    26341 cgccgtagtg ggtcagcggc ggctcttttg ctgacttgcg gcctaaacgc tccatgctgc
    26401 gggcgatcat atccagcatc gggtaaggga cggaacgaat acgacagtca atattcaact
    26461 cgtcgatcag cttctgcacg atgctgcgca gtgtgcgatg ctcgccgttg gtgatgttgt
    26521 acacacgccc ggaaggtagc ttatcgcagg cttcctggct tgccagccac attgcgtgca
    26581 cggcattttc atagtaggtc atatcgacca gcgcactgcc gccatgcggt aacagaatac
    26641 tgccgtagtg gtgcatcata tgcgccagac ggggaataaa gactttatcg tgcggtccga
    26701 acagactttg tgggcgcaga atagtaaagc gcgtttgtgg attcgcctgc gaaagcatat
    26761 tgatcacttc ttcgctggct gctttgctgc gggcaaactc gttggcgaag cggtgagggc
    26821 gaaaatcttc tttaatattg cgatggtggt gataatcgaa gtacagggag ggggaagaga
    26881 tatgaataaa gttacgcaca ccccaggcga cagcccattc acccaggcgg cgagtggcgc
    26941 gaacgttagc cagatcgaaa gcctgttgtg tcccccaggg tgaggtaaag ctggagcagt
    27001 gccacagcgt atcaatgccc gcgagcatca ctttagcttg tgatgaaacc agctcggtca
    27061 gatccgtcgg aacaaactct gcgcccattt tttccagcaa tttacccatt gcttcgttgc
    27121 gaccgctcgc acgcacgctg atgcctttct ggcataaaaa ctctaccgcg tttcgaccta
    27181 agccgctggt ggcgccggta accagtacct tcatatcaat ccactgttgt tgagaaaatg
    27241 acgtgcgcat tcttccgtga tttcccccat gatgcaatgg gaaacatgaa agaataacgc
    27301 aggttttgtc gattaatctg tgctttgttc tgccagtctg gcgatttgtt ttgccattcc
    27361 gcgaaaaata aacagatgcg cggggatcat caataaccag taaaacagcc ccggcatacc
    27421 gtgcggatgc cagaaagcgc ggacatcgat agtacgatag tcacctttat cttccagggt
    27481 aaaacacagt cgtcccagcc ccggcgcttt catgccaaat aacaacgtaa gttgcttttt
    27541 cggttcaacg acaatcactt tccagctatc caccgcatcg ccggtctgta aatattcgcg
    27601 ctccgggcgg cctttcgcca gattatgacc gatcgcgcgg tccatcaacg cccgtgtctg
    27661 ccacaaaata ttgccaaaga aataacgctc tttaccgccg atttggttca ccaccagcca
    27721 taaagccgca aggctggcgg acgttttaac ggtaaacccc gcctgtttgg cgaaataacc
    27781 gtactccggt cgccagcggg caaaggcctg agcgtcgtag ccccagtcgc tggagttgac
    27841 cagtttttct tcctctttca acgtgctacg taccgcgtca tcgaaagcga tcagccgttg
    27901 tgggatgagt gcacgtagcg cggtatcatc tgccagcaga tcgtgtttca gcccctgaat
    27961 caacgccctg gcggtggtgg gcggtacgga agtaatcaca ttgagaaacc acaccgaaat
    28021 ccagcgggtg gggaggggga tggggatcaa ccagcggcgc ttaccgctca ccgccataaa
    28081 atgttcaaac tgttgctgat aactgagcac ctctggtccg gcggcttcga agatgcggtg
    28141 ttcgttggct ggatggtcta acagcgccac cagatagtgc agcaagtttt ccagcgcgat
    28201 gggcgtggtg cgtgaacgta cccagcgtgg cggcgttaac actggcaggt tgtagaccat
    28261 atcgcgcatg acttcgaacg ccgctgaacc tgcgccaacg ataattccgg cccgaagttc
    28321 ggtcactggt acattcgctt cacgaagaat gtccgccgta gcctgacgag ctcgcagatg
    28381 atccgactgc tcatgtggcg gggcctgcaa cgaactgaga aagattaatt gcttaactgg
    28441 tacttcacgt agcgcatcgc ggacgttgag agccagctgg cgctcctgag cgataaaatc
    28501 gccgccttcg cccatgctgt gcaccagaaa atagacggta tcgatatcct gcaacagggc
    28561 cggaaggtta tccggccagc tgagatcgac tttatggcaa ctgacgtttg ccagttgcag
    28621 ctttgcaagc ctgtcgacat gacgtgccgc cgctaggatc tgatgccctt gctggctgag
    28681 tgtgcgcacc agatgctgac caatgtagcc actggcaccg agaactaaaa tgcgttgcgg
    28741 cacgtctctc tccttaacgc gccaggaatg cgcgccagtg ggcggcgact tccgccagtt
    28801 gttcgcgcga gacgtcaaga tgcgtcacca ggcggacaat cggcgaggcg ttaatcagca
    28861 cgtttctcgc tttcatgtat tcgcctaacg cggcagcatt ttcttccccg acgcgaacaa
    28921 acagcatatt agtgtcctga cgcatcacat ccgcgcctgc ttcacgcagt tgctccgcca
    28981 tccaggcggc gttgtcgtga tcttcctgca accgtgcgac gttattttcc agggcataca
    29041 tcccggcggc agccagaatc ccggactgac gcatcccgcc accggccatt ttccgccagc
    29101 ggatggcacg tttaatgtaa tcacgattac cgacgagtaa tgaaccgact ggcgtcccaa
    29161 gacctttcga caggcaaatg gtgaacgaat cacaatattg cgtgatctct ttcagttcgc
    29221 agccgtaagc caccacggca ttaaagatgc gcgcaccgtc aacatgcagc gccagattgc
    29281 gctcgcgggt aaattcccat gcttctttca ggtattcacg cggcagcact ttgccgttgt
    29341 gggtgttttc cagactgagt aatttggtgc gggcgaaatg gatatcgtcg ggtttgattt
    29401 tcatcgccac tttatccagc ggtagcgtgc cgtcggcagc cgcgtcgatg ggttgcggct
    29461 gaatactgcc cagcaccgct gcgccaccgg cttcaaacag atagttatgc gcggcctgac
    29521 cgacaatata ctcttcgcca cgttcgcagt gactgagcag agcgaccagg ttggcctgag
    29581 tgccggtagg cagaaaaatg gcggcttctt taccggaaag ctctgctgca tagtcctgca
    29641 gagcattaac ggtagggtcg tctccgtaaa cgtcgtcccc aaccggggcg gccatcatcg
    29701 cttcgagcat ggcgcggctc ggtcgggtaa cggtatcact gcgtaaatca atcatggcat
    29761 gtccttatta tgacgggaaa tgccaccctt tttaccttag ccagttcgtt ttcgccagtt
    29821 cgatcacttc atcgccgcgt ccgctgataa ttgcgcgcaa catatacagg ctaaaacctt
    29881 tggcctgttc gagtttgatc tgcggtggaa tggctaattc ttctttggcg accaccacat
    29941 ccaccaacac cggaccgtcg atagagaagg cgcgttgcag ggcttcatca acttcagagg
    30001 ctttttctac acggataccc gtaatgccgc acgcttcggc aatgcgggca aagttggtgt
    30061 cgtgcagctc agtaccgtct gtcaggtaac ctccggcctt catctccatc gccacaaagc
    30121 ccagcacgct gttattaaag acgataattt tcaccggcag tttcatctgc actactgaga
    30181 ggaaatcgcc catcaacatg ctaaaaccgc catcgccgca catggcgacc acctgacgtt
    30241 ccggctcggt tgcctgcgca cccaacgcct gcggcatggc gttagccatc gaaccgtggt
    30301 taaacgaacc taacaggcga cgcttgccgt tcattttcag ataacgtgcc gcccataccg
    30361 ttggcgtgcc gacgtcacag gtaaagatgg cgtcatcggc ggcaaaatga ctaatttgct
    30421 gcgccagata ttgcgggtga atggctttct cgctcggttt agctaaatcg tccagccctt
    30481 tgcgggcatc gcggtaatct tccagcgctt tatccagaaa cttgcgatcg gttttttctt
    30541 ccaccagtgg cagcaatgcg cgcagggttg atttgatatc gccgaccagc gccatatcta
    30601 ccttgctatg cgcgccgatg ctggctggat taatatcaat ctgaataatt ttggcatcgg
    30661 tcgggtagaa cgcacggtag gggaactgag taccgagtag cactaatgtg tcggcgttca
    30721 tcatggtatg gaaacctgac gagaatccga ttaacccggt cattccaaca tcatacggat
    30781 tatcgtattc gacatgctct ttaccgcgta gggcatgaac aataggtgct ttaattttcc
    30841 cggcaaactc aactaactct ttatgcgccc ccgcgcagcc gctgccacac atcagggcga
    30901 tattgctgga ataacgcagc agttgcgcca gtttgcgtaa ctcttcttct tccggcatca
    30961 cgactggctg tggcgcatga taccagtgcg tggttgcccc ttctggcgca ggttttaacg
    31021 ccacgtcgcc cggtaataca acaacggaaa cgccacggtt aagcaccgct ttgcgcatag
    31081 caattgccag cacttgtggg atctgctccg ggctggaaac cagctcgcaa tagtgactac
    31141 attcgcggaa tagctcttgt gggtgggttt cctggaaata gccgctgcca atttcgctgg
    31201 agggaatatg agcggcaatc gccagtaccg gaacgttatt gcggtggcaa tcgaataggc
    31261 cgttgatcaa gtgcaggttg cctgggccgc acgatccggc acagaccgcc agttctcctg
    31321 aaagttgtgc ttcagcgcca gcggcaaacg ccgccacttc ttcatggcgg gtggacatcc
    31381 actcgatggt gcccatgcga ttaagactgt cgctaagacc gttcagagag tcgcccgtga
    31441 ctccccagat gcgtttcacg cctgccgatt cgagtgtttt ggcgatataa gctgcaaccg
    31501 tttgtttcat ggttctccat ctcctgaatg tgataacggt aacaagttta gttcatctga
    31561 cggaggggga agggatggga gagaaaggag gcactaacgg ttaaatagcc cgatgaaagg
    31621 aatatcatcg ggcataaggc gattatgcga gaaccaaatc cccctgcgga tggcaggagc
    31681 aggccagtac gtaaccttca gcgatttcgg cgtcggtcag cgtcattgta ctgctcaccg
    31741 tatattcacc ggaaaccact tttgtcttac agcagccgca aacacccgca cggcaggcag
    31801 cgacaaccgg aacgttattg ctttccagcg cctccagtag cgtggtgcca accggggcgt
    31861 aaaattctcg tgccggttgc agtttggtga atttcagacc gctggtcgcc gcttctgcta
    31921 ctggggtgaa gaatttctct ttaaagaaac gcgtcacgcc gagcgctttc acttcctgct
    31981 ctacccaatc catatacgga gccgggccgc aggtcatcac ggtacgtgaa gctaagtcag
    32041 gtacatctgc cagcagttcg cgagtgaggc gaccagcgat aaagccttcg gtaacgttat
    32101 tttctgccac cagcgttacc ggatagttac gccactcatc ggcgaaaata acatcctgcg
    32161 gcgtacgcac gttgtagatc acccgcacat cggcctgtgg acggttcttc gcaagccagc
    32221 gacgcatcga cataatcggc gtgacgccgc agcctgccgc cagcaacagg aatttatctt
    32281 ctgctttatc gtcgcaggta aattccccca tcgcgtccga aagccagaga taatcaccgc
    32341 gttttacatc gcgcgtcagc cactgggagc cgacaccgtc atcaatccgc cgcacggtca
    32401 gggtgatata ttcactcacg cctggcgtgg aggaaatggt gtaagcacgc agcgtttccg
    32461 ctgagtttcg cacgctgacc agtgcatatt gcccggcgcg atatgggtag tgatcgtggc
    32521 aaatcaggga aatcgtccac acatccggcg tttcttgcgt aatgtgatga acctgtatcc
    32581 gccacgggca ttgattcgtt ggcatcgtca tcgacaaact ccttacgcgt tcaacagttg
    32641 cttcatgtct tcttcaacag tggtgataga acgcaggccg aatttctcgt tcagcaccgc
    32701 cagcaggtct ggtgtcagga aaccaggtgc agtcgggccg gtgacgatat ttttcacgcc
    32761 cagagaaagc agcgtcagca gaatgacaat cgctttctgt tcaaaccagg agagcaccag
    32821 cgacagcggc agatcgttca caccacagcc cagtttctct gccagagtga cagccagaat
    32881 aattgctgag taagcatcgt tacattgacc tgcatctacc agacgcggca gaccttcgat
    32941 atcgccaaac tcaagtttgt taaagcgata tttaccacag gcgagggtca ggatcaggca
    33001 gtcatccggc acgctggtgg cgaaatcggt gaagtagtgg cgctcgccgc gtgcgccgtc
    33061 acagccacca agcaggaaga tatgacgcag tttttcacgg ctaaccagat caatcagcgt
    33121 atcggctgca ccaagcagcg tctggcgacc aaagcccacg gtgatcaggt gaggaatttc
    33181 gctgtacggg aagcccgcca tttgttgcgc ctgggcgatg accgcagaga aatcttcgcc
    33241 atccagatga cgcacgccag gccagccaac gatgctgcgg gtccagatac gatcgtcata
    33301 agcgcctacg gttgggtcga tgatgcagtt cgaggtcatc acgatggggc cagggaaacg
    33361 agcgaactcc acttgctgat tctgccagcc gctgccgtag ttaccgacca gatgcttgaa
    33421 tttacgcagc tccgggtagc catgcgcagg cagcatttcg ccgtgggtgt agacattaac
    33481 gcccgtgcct tcggtctgtt ccagcaggtt gtagagatct ttgagatcgt gaccggaaat
    33541 cagaatgcat ttacccgccg tcgctttgac gttgacctgg gttggcgtcg ggtgaccgta
    33601 tttaccggtt tcgcctgcat ccagaatgct catcactttg aagttcatct ggccgatttc
    33661 cattgaacac tcaagaagcg cgttcatatc ggcaggccag gtccccagcc acgccatgat
    33721 tttatggtac tgggcataaa tatcgttgtc gtattgaccg agaacatgcg cgtgttccat
    33781 ataggccgcc gcacctttca ggccatacag gcacagcaga cgcaggccga gaatgttttc
    33841 gccaatcgcc gctttatctt tgttaggggt aaattctgct gcctgacgtt gcagctcgcc
    33901 gagatcatcg ctcaccagtt gcaggtcagc catcgggtta tcgacgcgcg cgttggcatc
    33961 tacagccagg cattgtgctt tcagcgcctc gcgcaaggca atcgcttcac gagcgtagcc
    34021 gacaatacgc ggagaatcga agttaacgtt ggtcagggtt gagaaaaagg cacgtggcgc
    34081 gaagctgtca acatcgtggt tgatgatgcc gtattcacgc gcttttaccg cccaggcaga
    34141 aagcccttgc agcgccgcga tgagtaaatc ctgaaggtca gaagtttccg ccgttttacc
    34201 acacatcccc tgcgcgtatg agcagccgtt ttctgccgga gtacggatag tttgttcaca
    34261 ttgcacacaa aacatgatca caccttttaa agttatattt aatatacatg tttaagggta
    34321 agacgcttaa cgcggggata aaagggattt ttcatgcaac tttaagggag attgatttag
    34381 cgcaattttg gcggcaggga actaccgcca gagaggtatt acgcagagaa aaaggcgatg
    34441 aggatcggca ctaacaggct aagaataaaa ccgtgaacaa ttgccgccgg gaccatatcc
    34501 agcccgccag tacgttgaag aacgggcagg gtgaaatcca ttgatgtggc accgcataag
    34561 cccagtgcag tagagcggct gcggcgaatc agcccaggga tcaacataat agcaatcagt
    34621 tcacgggcca gatcattaaa aaacgccgcg ctcccgatta ccggaccaaa agattcggtc
    34681 aataaaatac cggaaagaga ataccagccg aaaccggagg ccattgccag cgcggtattg
    34741 atggggagat caagaataaa ggcgttaatt aaaccaccaa ttaatgaact gacaaccacc
    34801 accacggcga caatcattcc ccggcgatta aggacaatct gctttaaggt catgccatta
    34861 ttgcgcaact gaataccaac gaggaaaagt agcaaaatta acgtgtattc actggcttcg
    34921 gtcgcgtgtt gtaagaaagc cagtccactc agaccaatgg caaaaccaat cactactacg
    34981 ccgcacagtt ttagcgactc cagcgccatc gcaatacgcg acgggagttt ttcttgctga
    35041 tggtggttgc gccacggcag gcctcgctcc agccacatca gggcggcaat attacacagt
    35101 aaaataacgg taatactgac ggcagaataa tgcagaatcg ccaacaggtt actggcgagg
    35161 ttatcgagaa acgccagact gatacccata aaaaagagaa taaggtaaac catccagctt
    35221 aatagctgat taatagcttt taacgcagct tgttggcgaa gcggaatgag gtaacccaca
    35281 atcaggggaa ccagaatgat taacagccca gaaaacatga aaatccagtc cttgcaaaga
    35341 agaagtcgaa atgcgcgatg acacactact gaaagcggaa gggcgagtaa agttgcaatt
    35401 aaaaggaaat gttatgcatc agaggggg
//
LOCUS       DAJTRD010000032        35226 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTRD010000032 DAJTRD010000000
VERSION     DAJTRD010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35226
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.032"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            88..864
                     /gene="exuR"
                     /locus_tag="OO315_001774"
     CDS             88..864
                     /gene="exuR"
                     /locus_tag="OO315_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5890714.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1209..1871
                     /locus_tag="OO315_001775"
     CDS             1209..1871
                     /locus_tag="OO315_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5890715.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1875..2258
                     /gene="mzrA"
                     /locus_tag="OO315_001776"
     CDS             1875..2258
                     /gene="mzrA"
                     /locus_tag="OO315_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5890716.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2405..2759
                     /locus_tag="OO315_001777"
                     /pseudo
     CDS             2405..2759
                     /locus_tag="OO315_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2797..3102
                     /locus_tag="OO315_001778"
     CDS             2797..3102
                     /locus_tag="OO315_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5890717.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3105..3509
                     /locus_tag="OO315_001779"
     CDS             3105..3509
                     /locus_tag="OO315_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5890718.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3499..3798
                     /locus_tag="OO315_001780"
     CDS             3499..3798
                     /locus_tag="OO315_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5890719.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3984..4280
                     /locus_tag="OO315_001781"
     CDS             3984..4280
                     /locus_tag="OO315_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5890720.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            4350..5336
                     /gene="yqjG"
                     /locus_tag="OO315_001782"
     CDS             4350..5336
                     /gene="yqjG"
                     /locus_tag="OO315_001782"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5890721.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5629..5997
                     /locus_tag="OO315_001783"
     CDS             5629..5997
                     /locus_tag="OO315_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5890722.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(6204..7100)
                     /gene="yhaJ"
                     /locus_tag="OO315_001784"
     CDS             complement(6204..7100)
                     /gene="yhaJ"
                     /locus_tag="OO315_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5890723.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            7205..7906
                     /locus_tag="OO315_001785"
     CDS             7205..7906
                     /locus_tag="OO315_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5890724.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7929..8093
                     /locus_tag="OO315_001786"
     CDS             7929..8093
                     /locus_tag="OO315_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890725.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(8305..9615)
                     /locus_tag="OO315_001787"
     CDS             complement(8305..9615)
                     /locus_tag="OO315_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5890726.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9643..10974)
                     /locus_tag="OO315_001788"
     CDS             complement(9643..10974)
                     /locus_tag="OO315_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5890727.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(11249..12613)
                     /gene="tdcG"
                     /locus_tag="OO315_001789"
     CDS             complement(11249..12613)
                     /gene="tdcG"
                     /locus_tag="OO315_001789"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5890728.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12685..13074)
                     /locus_tag="OO315_001790"
     CDS             complement(12685..13074)
                     /locus_tag="OO315_001790"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5890729.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(13088..15382)
                     /gene="tdcE"
                     /locus_tag="OO315_001791"
     CDS             complement(13088..15382)
                     /gene="tdcE"
                     /locus_tag="OO315_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5890730.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(15416..16624)
                     /gene="tdcD"
                     /locus_tag="OO315_001792"
     CDS             complement(15416..16624)
                     /gene="tdcD"
                     /locus_tag="OO315_001792"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5890731.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16650..17981)
                     /gene="tdcC"
                     /locus_tag="OO315_001793"
     CDS             complement(16650..17981)
                     /gene="tdcC"
                     /locus_tag="OO315_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5890732.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(18003..18992)
                     /gene="tdcB"
                     /locus_tag="OO315_001794"
     CDS             complement(18003..18992)
                     /gene="tdcB"
                     /locus_tag="OO315_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5890733.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(19091..20029)
                     /gene="tdcA"
                     /locus_tag="OO315_001795"
     CDS             complement(19091..20029)
                     /gene="tdcA"
                     /locus_tag="OO315_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5890734.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            20219..20563
                     /gene="tdcR"
                     /locus_tag="OO315_001796"
     CDS             20219..20563
                     /gene="tdcR"
                     /locus_tag="OO315_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5890735.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20819..21359
                     /locus_tag="OO315_001797"
                     /pseudo
     CDS             20819..21359
                     /locus_tag="OO315_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21381..21728
                     /locus_tag="OO315_001798"
     CDS             21381..21728
                     /locus_tag="OO315_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890736.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21897..22547
                     /locus_tag="OO315_001799"
     CDS             21897..22547
                     /locus_tag="OO315_001799"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890737.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(23168..23544)
                     /gene="rnpB"
                     /locus_tag="OO315_001800"
     ncRNA           complement(23168..23544)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO315_001800"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(23577..24722)
                     /gene="garK"
                     /locus_tag="OO315_001801"
     CDS             complement(23577..24722)
                     /gene="garK"
                     /locus_tag="OO315_001801"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5890738.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(24819..25709)
                     /gene="garR"
                     /locus_tag="OO315_001802"
     CDS             complement(24819..25709)
                     /gene="garR"
                     /locus_tag="OO315_001802"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5890739.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(25739..26509)
                     /gene="garL"
                     /locus_tag="OO315_001803"
                     /pseudo
     CDS             complement(25739..26509)
                     /gene="garL"
                     /locus_tag="OO315_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(26525..27859)
                     /gene="garP"
                     /locus_tag="OO315_001804"
     CDS             complement(26525..27859)
                     /gene="garP"
                     /locus_tag="OO315_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5890740.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            28234..29805
                     /gene="garD"
                     /locus_tag="OO315_001805"
     CDS             28234..29805
                     /gene="garD"
                     /locus_tag="OO315_001805"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5890741.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(29857..30681)
                     /gene="agaR"
                     /locus_tag="OO315_001806"
     CDS             complement(29857..30681)
                     /gene="agaR"
                     /locus_tag="OO315_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5890742.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            30930..32210
                     /gene="kbaZ"
                     /locus_tag="OO315_001807"
     CDS             30930..32210
                     /gene="kbaZ"
                     /locus_tag="OO315_001807"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5890743.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            32233..32706
                     /gene="agaV"
                     /locus_tag="OO315_001808"
     CDS             32233..32706
                     /gene="agaV"
                     /locus_tag="OO315_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5890744.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            32717..33496
                     /locus_tag="OO315_001809"
     CDS             32717..33496
                     /locus_tag="OO315_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5890745.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            33486..34295
                     /locus_tag="OO315_001810"
     CDS             33486..34295
                     /locus_tag="OO315_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5890746.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            34382..34816
                     /locus_tag="OO315_001811"
     CDS             34382..34816
                     /locus_tag="OO315_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5890747.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            34813..>35226
                     /locus_tag="OO315_001812"
     CDS             34813..>35226
                     /locus_tag="OO315_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5890748.1"
                     /translation="MTHVLRARRLLTEEGWFDDHQLRVADGVIAAIEPIPVGVTERDA
                     ERLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHP"
BASE COUNT         9123 a         9092 c         8414 g         8597 t
ORIGIN      
        1 aatcatcaaa tttatatatg accaatggca tacccatgaa taaagtggta tgataacttc
       61 gtccgcattc cactccggga gacgcatatg gaaatcactg aaccacgccg tttgtatcaa
      121 caacttgctg ctgacctgaa agagcgcatt gaacagggcg tctatctggt gggtgataaa
      181 ctgcctgccg aacgctttat tgccgatgaa aagaacgtca gccgaacggt agttcgtgaa
      241 gccatcatca tgctggaagt tgaaggctat gtggaggtgc gtaaaggttc aggtattcat
      301 gtggtttcca accagccgcg tcatcagcag gcggctgaca ataatatgga attcgccaat
      361 tacggtccgt ttgagttgct tcaggctcgc cagctcatcg aaagtaatat tgccgagttc
      421 gcggcaactc aggtaacgaa acaagacatc atgaaactga tggccattca ggaacaggcg
      481 cgcggcgaac aatgcttccg tgattccgag tgggatttac agttccacat tcaggtagcc
      541 ctggcgacgc agaactccgc cctggcggcc atcgttgaaa aaatgtggac ccagcgtagc
      601 cataacccgt actggaaaaa actacacgaa cacattgatt cccgtaccgt tgataactgg
      661 tgtgatgacc acgatcaaat tctcaaggcg ttaattcgca aagatcctca tgctgctaag
      721 ctggcaatgt ggcagcatct ggaaaacacc aaaatcatgt tatttaacga aaccagcgac
      781 gatttcgagt tcaatgccga ccgctatctg ttcgccgaaa acccggtagt gcatctcgat
      841 acggccacca gcggcagcaa atgaaattcc tgtccgacag gcgctgcaat gcgcctgttt
      901 gtctcgtcgg taagcgaaaa gtataaagtg tcagcctgtg taaatcctct cgccaccctc
      961 ccctgcaatc agcaaaatca gactccacgg acatgtaatt ttgataacga acaacgttga
     1021 cctttgttac aattagattc aatttgaatt tatgcttttg aatgctttct tatctcacgt
     1081 tttaacaggg aatagttcag gctgtgttga tgtatcaaac ccgcagaaca taccaaaaca
     1141 gcaataacat tgcggtagtg catcttttaa aaccagcgtg gcgttaaccg attcaccagg
     1201 aataatgaat ggaacttttg acccaattgc tgcaagccct gtgggcgcag gattttgaaa
     1261 ccctggccaa tccatcgatg attggcatgt tgtattttgt cttgtttgta attttgttcc
     1321 ttgaaaatgg cttgcttccg gcggcctttt taccgggcga cagtttactg gtattggtcg
     1381 gcgtgttgat tgcgaaaggc gcgatgggtt acccgcaaac gattctgctg ctgaccgttg
     1441 ccgccagcct cggttgctgg gtcagctata ttcaggggcg atggctgggc aatacccgca
     1501 ccgtacaaaa ctggctatct catttacccg cgcattatca tcaacgcgca caccatcttt
     1561 ttcataaaca cggtttatcg gcgctgttaa ttggtcgctt tattgcattt gtcagaacac
     1621 tgctgccgac gattgccggg ttatcagggc tgaataacgc gcgctttcag ttcttcaact
     1681 ggatgagcgg tctgctgtgg gtattgatcc tgacaactct gggttacatg ctcggcaaaa
     1741 cgccggtatt tttaaagtac gaggaccagc tgatgtcatg cctgatgctg ctcccggtgg
     1801 tgctgctggt gtttggcctg gcaggttctc tggtcgtgtt atggaaaaag aaatatggaa
     1861 atcgggggta agggatgcaa atacctcgca tgtcgcttcg ccagctagcc tggtccggca
     1921 ctgttttact tctggtcggc acgctgttgc tggcctggtc cgcggttcgc cagcaagagt
     1981 ctacgctggc gattcgtgcc gttcatcaag gcacaacgat gccagacggt ttttcgatct
     2041 ggcatcacct tgacgctcat ggcattcctt tcaaaagtat cacccccaaa aacgacactc
     2101 tgttaattac atttgactcc agcgaccaga gcgccgccgc aaaagcggtc ctcgacagaa
     2161 cattgcccca tggctacatc attgcgcagc aggacaataa cagtcaggct atgcagtggc
     2221 tgacccggtt acgggataac tctcatcgct tcggataact tcctgttatc cgaaacatat
     2281 cactcacttt ggtgatttca ccgtaactgt ctatgattaa tgaggcggtg gttaacacta
     2341 ccgcttcgtt gtctggacaa tcgttccttt gtaataggtc cgaatcacaa tggaaggttc
     2401 aagaatgaaa taccgcatcg ctttagctgt ttctctcttt gctcttagtg ccggtagtta
     2461 tgccactacc ctgtgtcagg aaaaggagca aaatatcctt aaggagatca gctatgccga
     2521 aaaacaccaa aaccagaatc gtattgacgg tctgaataaa gccctgagtg aagtccgggc
     2581 caactgttca gatagccagc tgcgtgccga tcatcacaag aaaatcgcaa agcagaaaga
     2641 tgaggtggcg gaacgccagc aagatttagc cgaggcgaag caaaaaggcg atgccgatag
     2701 aacggaaact ggcagaagcg caggaagagc tgaaaaagct ggaagcgcgc gactactaac
     2761 tcacaatagt cactacttac tcacctggag aaaactatgt cgaaagaaca cactacggaa
     2821 catctgcgtg ctgagttgaa atccctttcc gatacgctgg aagaggtgct tagctcatct
     2881 ggcgagaagt cgaaagaaga gttgagtaag attcgtagca aagcggagca ggcactgaaa
     2941 cagagccgtt atcgcctggg tgaaaccggt gatgccatcg ccaaacaaac ccgtgtcgcg
     3001 gcggcgcgtg ctgatgagta tgtgcgtgaa aatccgtgga ccggcgtggg aattggcgct
     3061 gcaatcggtg tagtgctcgg cgttctgctg tcgcgtcgtt aattatggcg gacactcatc
     3121 acgcgcaagg gcccggtaaa agcgttctgg gcatcgggca gcgaattgtt tccatcatgg
     3181 ttgaaatggt ggaaacacgt ctgcggctgg cggtggtgga gctggaagag gaaaaagcta
     3241 atctctttca acttttactg atgctgggcc tgacgatgct tttcgctgca tttggtctta
     3301 tgagcctgat ggtgctaatt atttgggcgg ttgatccgca atatcgcctg aatgcgatga
     3361 ttgccaccac cgtggtgttg ctgctactgg cactgattgg cggtatctgg acgttacgta
     3421 aatcgcgtaa atccaccttg ctgcgccata cacgccatga gctggcaaac gatcggcaac
     3481 tgctcgagga ggagtcccgt gagcagtaaa gtcgaacgtg aacgacgtaa ggcgcaactg
     3541 cttagccaga tccagcaaca acggctggat atttccgcca gtagtcgtga atggctggag
     3601 gcaacaggcg cttacgatcg tcgctggaat atgctgctaa gtctgcgctc ctgggcgctg
     3661 gttggcagta gagtgatggc gatctggacg attcgccatc ctaatatgct ggtccgctgg
     3721 gccagacgcg gttttggcgt atggagcgcc tggcgtctgg ttaaaacgac cctcaagcag
     3781 caacagcttc gcggttaact tccctctggc cggagcaatt ccggccttat ccatcaaatt
     3841 ttttgaatat ttttgacagt tttccttgct aacaatcatc attcaccacg tttatgattc
     3901 tctccatcga cagcaacgac gctaatagcg cgcccttgca caaaaaaaca atcagcagcc
     3961 tgagtggcgc agtggagagt atgatgaaaa aattagaaga tgttggtgta ctggtagcgc
     4021 acattttaat gccgattctg tttattaccg caggctgggg aaaaatcacc gcttactctg
     4081 gtacccaaca atatatggaa gcaatgggtg tcccgggttt tatgctgctg acggcatttt
     4141 tatttcacag caactttgct gaaggcgtca actcgctgat gttcatgaaa aacctgacaa
     4201 tttctggcgg attcctgctg ctggcaatta ccggtccggg cgcgtatagc atcggccgcc
     4261 tgctgaataa aaagtggtaa gcacgcgcta tacttaacga taaaaaacga ggaggaagct
     4321 cctcgttttt gctattggag gagagaaaaa tgggtcaact gattgacggc gtctggcatg
     4381 acacctggta cgataccaaa tctaccggcg gtaaatttca acgttcagct tccgcatttc
     4441 gtaactggct cactgccgat ggcgcacctg gccccactgg cacaggcggt tttatcgcag
     4501 agaaagatcg ttatcatctc tatgtttcac tcgcctgccc gtgggcgcac cgcacgctga
     4561 tcatgcgcaa actcaaagga ctggaaccgt ttatttccgt ttccgtagtg aacccgctga
     4621 tgctggaaaa cggctggacc tttgatgaca gttttccggg agcaaccgga gatacgctct
     4681 atcaacatga atttctgtat cagctttatc tccacgccga tccacactac agcggacgag
     4741 ttactgttcc cgtgctgtgg gacaaaaaga accacaccat cgtcagcaac gaatcagcag
     4801 aaatcatccg catgttcaat accgcgtttg atgcgctggg cgcaaaagcg ggtgattact
     4861 acccaccagc cctgcaaacg aaaattgacg aacttaacgg ctggatttat gacaccgtta
     4921 acaacggcgt gtataaagct ggttttgcca ccagccagca agcctacgac gaggcggtgg
     4981 cgaaagtgtt tgaatcgctg gcgcgactgg agcagatttt aggccagcat cgttacctga
     5041 ccggcaacca gctaaccgaa gccgatattc gcctgtggac tacgctggtg cgttttgatc
     5101 cagtgtatgt gacccacttc aagtgtgata agcaccgcat cagcgattac ctgaatctgt
     5161 atggcttcct gcgcgatatc taccagatgc cggggatcgc cgaaactgtc aatttcgatc
     5221 acatccgtaa tcattacttc cgcagccata agaccatcaa ccctacgggg attatttcaa
     5281 ttggtccgtg gcaggatctc gatgaaccgc atggacgaga tgttcgcttc ggttaaaaaa
     5341 aggcacctta cgaggtgcct ctgataatta gctaagtgca tgttgttcat gtcggatgcg
     5401 gcgtgaacgc cttatcaaac ctacaaagcg tttatattca atatgttaca atgaccgcgt
     5461 aggccagata agcgtagcgc atctggcgat tttgcgcttg ccatcagtct tagcgcgcct
     5521 tcacggcact ttattaattc cttgaacgaa tatttactgc catttcattc acgtctattc
     5581 ttaatttgct gctttaaaaa caagtgattg agcaaaattg aggcaaaaat ggactggtat
     5641 ctgaaagtac taaaaaatta tgtcggtttc cggggtcgtg cgcggcgcaa agagtactgg
     5701 atgtttattc tggtcaacat catctttacg ttcgtgctgg ggctgctgga taaaatgtta
     5761 ggctggcaac gcgctggtgg cgaaggcatc ctgacgataa tctacggtat tctggtgttt
     5821 ttaccgtggt gggcggttca gttccgccgc ctgcacgaca ccgaccgctc ggcgtggtgg
     5881 gcactgctat tcttaatccc gtttatcggc tggcttatta tcatcgtctt taactgccag
     5941 gcgggtacgc caggcgaaaa ccgctttgga ccagatccaa aactggaaca agaataatga
     6001 ttaagcacct tgtaaaaggt gcttcaaatt gttgacaaag tgcgctttgt ttatgccgga
     6061 tgcggcgtaa acgccttatc cagcctacaa attgccaaaa ttcaatatat tgcagtgact
     6121 gcgtaggcct gataagcgta gcgcatcagg cagttttgcg tttgtcagca gtcagaagca
     6181 ccttgtgaaa ggtgcttaat agattatttt ccagcgaaca actttggaat ttcccgcaga
     6241 caccaggatt tcgcttcccc catactgtca cgacgccagg ccataataat atcgatctcg
     6301 ctggtcgatt ccgggctgac gacacgcaac cgcccttccg caatatcttt ttcgaccatc
     6361 ggatacggca tcgtcgccac gccaagcccc gccagtaatg cctgacgttt atcttcaatc
     6421 gtgctcaccg ttaagcgcgg ctgtttgtcc agcagctgta cggtcaacac cgggcgctca
     6481 cgagcggtat ccgctaccgc aattccacga tatttcacac gcgtcacttc agataacggt
     6541 tccggctcct gatgaatcgg gtgatcaggc gcggcaacgt agacgttcat taacgtatag
     6601 agcttgcgcg agttgatctc cgacgaggaa cgaaaatgca tatccggcgc gataacgata
     6661 tccgcccgcc cctgctccag ccgttcccac gcccccgcca gcacttctgt gatgattgcc
     6721 agttgggtat tggcttttgc cgccagtttg tcgattaacg ggaaaaaggc aggtgtcggt
     6781 accagcgctt cggtcacaat ggtgagatgc gtttcccaac cgcgcgcgag agcttccgca
     6841 tcagtagtca gtttatctgc ggcttccagc aaaacgcgcc ctcgctccag caacatcctc
     6901 ccgacattgg tgaatttggt gcgatggccc gagcggtcaa acagcaccac atccagctct
     6961 tcttccagtt tttgcatggt gtagctaagt gcggaaggca cgcgtcccag ctcatccgct
     7021 gccgccgcaa aactgccccg gcgatcgatc gcatccataa cccgtagtgc ttccagcgtt
     7081 aatgcccttt ctttggccat ttcgttctca ttcaggaaat ttgaacatac cgggcagaat
     7141 atctggctaa caatgtagcg tccagcccct taccataaaa ggaagtaaag agaggtcaag
     7201 aattatgatt actacccgaa ctgccaggca gtgtggacaa gcagactacg gatggttgca
     7261 ggcccggtat actttttcct ttggacacta cttcgacccg aaattgttag gctatgcctc
     7321 cctgcgtgtg cttaaccagg aagtgctggc cccaggtgcc gcctttcagc cgcgaaccta
     7381 tcccaaagtc gatattttaa atgtgattct ggatggagaa gcagagtatc gcgacagcga
     7441 aggcaatcat gttcaggcca gcgccggaga ggcgttgctg ctctctaccc agccgggtgt
     7501 cagctatagc gaacacaatc tcagcaaaga caaaccgtta acgcgaatgc agctttggct
     7561 ggatacctgc ccgcagcgag agaatccgct gattcaaaag ctggcgctta atatggacaa
     7621 gcagcaatta atcgcctcgc cagagggtgc gatgggaagc ctgcaattac gccaacaagt
     7681 gtggctgcac catatcgtgc tcgacaaagg cgaaagtgcg aatttccagt tgcatgggcc
     7741 acgcgcgtat ttgcaatcga ttcacgggaa atttcatgcg cttacgcatc atgaagagaa
     7801 agcggcgcta acctgcggtg atggggcgtt tattcgtgac gaggctaaca ttacgctggt
     7861 tgccgattcc ccactgcgcg ctttgctgat agatttgcct gtctagttgt ttttacaggg
     7921 agatgatgat gagtaaaaaa tcgaccaaaa agcgccagcc agcgaagccc gtggtggcga
     7981 aagaacctgc tcgcaccgcc aaaaattttg gctatgaaga gatgttgagc gagctggaag
     8041 ctatcgtcgc ggatgctgaa acgcgtttag ccgaggatga agctaccgcg taaagacagg
     8101 ttgccggatg cggcgtaaac gccttatccg gcctacaaaa cacaacaaca tcaacaaatt
     8161 aaacaccccg taggccggat aagatgcgcc agcatcgcat ccggcatcag gtacaaaaca
     8221 caacaacatc aacaagttga gcaccccgta ggccggataa ggtgcgccag catcgcatcc
     8281 ggcacaatac tcaaaacctg gcgtttatct ggccttgctc gccataatct cgataatctg
     8341 ccgatccgtt tgctgcatcg aatggcttgc taacgcacac aggttggcaa tcgactgctc
     8401 aacatcatgc gccacgatcc cttcattgcc ggtcacggcg gtatcatcca gcgccattaa
     8461 caccgctttc cacgcagccg aagcactggt cgaaaccttc atcgcgcagc tgttcgacgc
     8521 accatcgcaa atcatgccgc tgacatcgcc gatcatactg ctgatcgcca tcgagatagt
     8581 ttcataacgc ccatccacca gccatgccat cccggcggcg gcccccattg ctgcggtcgt
     8641 tgcggcacaa agtgcagaca aacgcggtaa ctggttatgg atgtaaattg cgctcaaatg
     8701 agaaagcatc agcgcacgcg ccagtcgttc atcatccgct ccgaagtgtt ctgctacaac
     8761 caccacaggc attattgcag tgatcccctg gttacccgag ccggagttac tcatagccgg
     8821 aagcgtagcg ccgcccatac gcgcatcgga tgccgcgctg gtacgaatca caatggatga
     8881 agagagatct ttcgccagca aaccgcgctc gcactgtttt tccagcgtcg cgccaatatg
     8941 cagcccccac ttaccgctca aaccttcctg cgataacgca caatttagct tcgcggaatc
     9001 gagaataaag cggatcgccg caaatgggac ttcattgacg aacttcagga tctcagccag
     9061 cgtcgttctg gaaagcaccg tcagcggcga ctcttgctcg ccctctgcca cacacgcctg
     9121 gtgggtaaac accacaccat tgtgcgtctc aatatgcaca atgttggtat gcccgccgac
     9181 gatggtgaca cacgcccact tctcaccgtt ccagactttg gcgcgtgaga agaggatttc
     9241 attgcaaggt tcctggatct taacagagac tttccccgcc gccagcagtg ctttggcatc
     9301 ggtaattgcc tgcgcagttg cgtctttcag cacttccagc ccggcgttgg catttccacc
     9361 taacgccccc agcgccgccg caatcggcag ccccaccatt cccgtgccgg gaacggtgac
     9421 gcccagaccg ttcttcatca gatttggcga aacccaggct tctacacgtt caaccggacc
     9481 ttccagttct gccgcagcaa ccgccgccgc cagcgccagt gaaatcggtt cagtacatcc
     9541 cagcgccggt tttacttcct cctgaacggc gaggatgtaa cgctgccata acggatttaa
     9601 agtcgaatca aacatattaa aaaccttaaa atttcaggta aatcaagaaa atgccaggaa
     9661 cagagaaaca caaagcaaca aaccagtgac gataatcagg tacagagaca tccctttgta
     9721 tttgtgcaat gccggtactt tgtaaaccag ccacgccggg atcaggcacc ctaccatgcc
     9781 gaaaatcggg ctacagatag aggtgaagct caacaccggt gcgttcagta cgatggcgct
     9841 ccaggccagc aaaatggcga aaatcatgat gccgcgctga acaagatttt cgttaatctt
     9901 ctcggcaggc atcttgcgac gcaggatgtt cattacgatc ccttgcgttg cttcgcgaaa
     9961 gcctaaatag acgccaaaga atgcagtcat gacagcaaag atattgagaa tgacgctgac
    10021 cactttcacc cacgctgcgc cgtcaccgct aataaactgt gcggcaatcg ccagcgcgga
    10081 aatattctgc tcgtaggctt ttaccgcttc gtcatgtccc atcgccagcg tgaacgacac
    10141 ggcgtagaaa aagacggtga caaacaaaat gccaaacgcg atattcattg cccgcaatgc
    10201 tttatgccgc gccacttcaa ttgatttttc ccgcgagcga taagagatca ccatcgggct
    10261 taacgtctgg ataaacagaa tcgacgtcag ggtaaacggc agcgtaataa tggcgttttt
    10321 caccagcagc cccagcggcg gtagcgaacc gacgttatac agatgccaca ttcccaccat
    10381 cgacacgccc agcgcagcga ctaccagcag cttggtcagc accatgtcgg tcgaaatttt
    10441 gaataacaat ttctcgccgc gtgaagagat cgccaccaga atgcaaatca gtaccagacc
    10501 ataaaaggga ctgtctgaca gcaacccttc cgtcacgccg aaggtatgca gataggaagc
    10561 actatcgttg gtgatggcgg tggaatagac gaacatccaa atcaccagca ttacgaaata
    10621 gagcgcacct aacaggatgc cccagttttt acctaaataa ccgctaatga cactcgggta
    10681 atctttacat tctggtgatt ctgccagcgt attaataaac aaccgctgaa acagatacat
    10741 tgccgggtaa ccaatcaccg atgagagcaa aaatacccac aatcccatca aaccgacctg
    10801 caccgggaga aaaacaatcc ccgcgccaat cgccatcccg atactcataa tcacccagcc
    10861 ggtgtcagta ctgtcgaatt tgatcgcttc tcgccactcg ctctcactca ttccagcacg
    10921 acccgccggg gtcgaagcgt ctgcaatgac gcctttattc gatgcaattt ccataatttc
    10981 tcgctcaata ttttgtaggg cttattattt ttttccgagc cgcatcaagg cgatatgcgg
    11041 tctacgtttt tgcaggcgag ttattagaat aaagaaatga tacgcgcgag gtcagagaaa
    11101 atcttcacaa tcaagacttt aaaataacgg agatggataa gaattttcta ctaaattaat
    11161 cacaagagaa tattcattct ctatttgcgg cagatcacaa aaaaaaggtg cacatttgtg
    11221 cacccaagga tgaaagctga cagcaatgtc agccgcagac cactttaatg gccagtcctc
    11281 cgcgtgatgt ttcgcggtat ttatcgttca tatctttgcc ggtttcatac atcgtctcga
    11341 tcactttatc gagtgaaaca cgcggtgccg aggtgcggcg catcgccatc cgcgcggcgt
    11401 ttactgcttt cacggcatta atggcattac gttcaatgca cgggatttgt acctgtccgg
    11461 caaccggatc gcaggtcagc ccaaggttat gctccatcgc gatttccgcc gcattgcata
    11521 cctgcgccgg actgccgccc agtagttcag ttaaccctgc cgctgccatt gaacaggcca
    11581 cgccaatctc cccctgacag ccgacctccg cgccagagat ggaggcgttc attttataca
    11641 gcgcgccaat agcccccgct gccagaaaat agcgggcaat tgaccgctcg tttaccggac
    11701 gacggaactt atcgtaataa gccagcactg ccggaataat gccgcacgca ccgttagtcg
    11761 gtgccgttac cacgcgcccg ccagctgcgt tttcttcact aaccgccagc gcgtacatgt
    11821 tgatccagtc gatgacattc atcggatcgt tagagatgtt atcgctagaa accagctgac
    11881 gacgcagcgc tacggcacga cacggcacat tgagcggacc aggcagcacg ccttcagtgt
    11941 tcatcccgcg ttcaataccg tcatgcatca cttgccagat acgggcaaac cctgcgtcaa
    12001 tttccgcttt gctgcgcagc gccagctcgt tgtgcatcat caggccagat atagacaggc
    12061 cgttgtaatc acacattttc agcagttcac ctgctgagtg gaaatcgtaa ggtacggacg
    12121 tttcgacatc gtgcgacagg ccgaagtgtt cttcttcgac aataaacccg ccgccgacag
    12181 agtaataggt tttacttaat agcgcttcct gtcccttcca ggcagtgatc cgcattccgt
    12241 tctcatggcg aggcaacatt tcgggatgga agataatgtt ctttgctaca ggaaaatcaa
    12301 caatatgcgc accagatgcc actggcagcc gcccgctgcg cgttactaac tctataaatg
    12361 cagggatctc atcaatgaca acatcctgcg gactgtttcc tgccagtccc atgataatgg
    12421 cgacatccgt ggcatggcct ttgcccgtca gtgacaacga cccgtacaga tcgaccacaa
    12481 tatggctcgt cgcggttaat aagccgctac tttccagccg atcaataaaa ctttttccgg
    12541 cattcattgg ccccacggta tgcgaactgg agggaccaat cccaattttg aaaatatcga
    12601 atgcactaat catatccaca ccctcggatt gccgttcagt gaagtggagc ggaacgacct
    12661 tgcgaccgtc ccgctcacga ggctttacgc actacgtact gcgatagctt caatttccag
    12721 cttcacatct ttcggcaaac gcgcaacctg cacacagctc cgggtcgggt aggtcgcctg
    12781 atgctcatcg aagaactgct tatagacttc attgatggtg gcaaaatcat tcagatcggt
    12841 gatgaacacg gtcatcttga tgatatcgcc cacgctcagc ccggcagcaa ccacgatcgc
    12901 tttgacgttt tcgaggctta aacgcgcctg atcttgcaca tcagccggga tctcaccggt
    12961 ctgcgggcaa accggaattt gcccggaggt gaagaccatg ctgcctaaat caacgccctg
    13021 aacataaggg ccgattgcgc ctggggcacg ttgcgtttcg ataatctttt tcatacatcc
    13081 tccggcgtca gagcgcctgg gtaaaagtac gtgaaataac atcctgttgc tgttcacggg
    13141 tcagtgcgtt gaagcgcacg gcgtagccag agacacggat tgtcaggtta ggatattttt
    13201 ccgggtgctc gatggcatcc agcagcattt cccgattcat tacgttgacg ttgaggtgtt
    13261 gaccgccttc gacatccgct tcgtggtgga aatacccatc cagcaggccg acaaggttgg
    13321 ttttacgtac tggatcttct ttgcccagcg ccgcaggaac gattgagaag gtgtacgaga
    13381 tcccatcttt ggcgtaggtg aacggcagtt tcgccaccga cgtcaatgag gccacggcac
    13441 ctttgcggtc acgaccatgc atcgggttag cgcccggcgc gaacggtgtt ccggcgcgac
    13501 gaccgtccgg cgtattaccg gttttctggc cgtacaccac gttagaagtg atagtcagaa
    13561 tcgactgggt agggacggcg ttgcgatagg ttggcagcgc tttaattttc ttcataaagc
    13621 gttcaaccag gtcgcaggca atgctgtcta cgcgctcgtc gttgttgccg tactgcggat
    13681 attcaccgtc gatttcaaag tccaccgcca ggccgttttc gtcacggatt ggtttcacgc
    13741 gggcatattt gatggcagac agggagtccg tcgccaccga caggcccgcg atgccgcatg
    13801 ccatagtgcg atagacatca cgatcgtgca gcgccatcag cgaagcttcg tagctgtact
    13861 tgtcgtgcat gtagtgaatg atattcagcg cgctgatgta ctgcaccgcc agccagtcca
    13921 tgaagtgatc gaggctgtcc atcactttgt cgtaatccag cacgtcgtcc atcagcggtg
    13981 ctgttttcgg cccgacctga atcttcagct tctcgtccac cccgccgtta attgcgtaga
    14041 gcagcgtttt cgccaggtta gcgcgtgcac caaagaactg catttgctta ccaatcacca
    14101 ttgggctgac gcagcaggca atcgcgtaat cgtcgctgtt gaagtcagta cgcatcagat
    14161 cgtcattttc atactgcaag gaagaggtga cgatcgacac ctgcgcggca tattttttga
    14221 aggcaatcgg taattcttcc gaccaaagaa tggtcaggtt aggttccggt gccggcccca
    14281 tagtgtgcag ggtgtgcaaa tagcggaagg agtttttggt caccagcgta cgaccgtcca
    14341 gccccatccc gccgatcact tccgtcgccc agattgggtc gccggagaac agcgaatcaa
    14401 attccggtgt acgcaggaag cgtaccatac ggatcttcat gatgaagtga tcgatcagtt
    14461 cctgtgcctg ctgctcattg aggacgccag ctttaaagtc gcgctcaatg tagatatcca
    14521 ggaacgatgc cgtgcgcccc agcgacatcg cgccgccatt ttgcgatttc actgccgcca
    14581 gataagcgaa gtagagccac tgcaccgctt cctgtgcatt ctgcgccggg cgagagatat
    14641 caaagccata tttcgccgcc atttcctgaa tctgcaacag cgcatgacga tgctctgcca
    14701 gctcctcacg cagacggatg gtggcttcca gatcctcgcc tttttccaga cgagactgga
    14761 gatcggcaaa ttgcagttcg cgttcacgca ccagataact gatgccatac agcgctacgc
    14821 ggcgatagtc accgataatg cgcccacgac catagccatc tggtaaaccg gtcagcacgc
    14881 cagatttacg gcagcgcagc atatccggtg agtaaacatc aaatacgccc tggttatggg
    14941 ttttacgcag atcggtaaac agatattcaa attcactgtc catttcacgg ccataggcgt
    15001 ggaatgaact tttaatcata ttaatgccac cgaacgggtg tagcgcacgt ttcaacggcg
    15061 catccgtttg caggccaaca attttttcca gcggctggtt aatatatccc gcatcatgag
    15121 cggtaattgt ggtggcaata ttggtatcga aatcaaccgg cgcgtgggtt gcgttttcga
    15181 tacggatgcc ttccattacc ttttcccaca attccgtggt ggcaggcgtc gcttcggcga
    15241 gaaaagattc atcgccttca tacggtgtat agttatgttg aataaaatcg cggacattaa
    15301 tttcgctttt ccagtccgta cctttaaagc caagccatgc gtcagcgtac agcttatcgc
    15361 tggtatcaat atctaccttc atgaaaaata atctctctac aatacttcaa ctaaattatg
    15421 caaattctgc gggcgcgtta actttaccta aatgaatggc atccaaagca atcatttttt
    15481 cttcgttagt cggaataacg acgcaaatga catgcgcatt ttcactggaa acaattcgct
    15541 cgccaaaaga gttagaacga ttattcattt ctgtatcaat ctctacgcct aatacagcca
    15601 aatgttccat gaccagacga cgaattaaac ttgaattctc tcctattccg ccggtgaata
    15661 taattccatc cagccgacgt aatgaagctg catgtccggc aatatgacgg gcaattcggt
    15721 gaacaaaggt tttaattgcc agttgcgcgc gttcgtgacc ttcatgccag gctttttcca
    15781 gaacacgtaa atccgaagaa agaccggaaa tacctaataa tcccgactct ttattcacta
    15841 cgcgttccag gtcacccagg ctctggttgg tttggctggc gacccaggac atcgcaccaa
    15901 agtcgacatc gccactgcgg gtacccatca tcaagccttc cagcggcgtc attcccattg
    15961 aggtgtcaac actctgaccg ttgcgaactg cgcagattga cgcgccattg ccaagatgcg
    16021 ccacaaccag gccggagtca tcttccgcca gattcagcag cgaatgtgcg cgctgggaaa
    16081 catagcggtg cgacgtgccg tggaaaccat aacggcgtac acctaactct tcataatatt
    16141 tccacggcag gccgtataaa taagcttccg gagccatcgt ctggtggaaa ctggtatcaa
    16201 ataccgccac ctgagttacg cccggaaata attgctgcgc cgattcaata ccacttaaat
    16261 ttgcgtaatt atgcaggggt gccagtggag aaacgcgacg gatattatca atgacttcat
    16321 cggtaataat ggcggactcg gtaaaaatac tgccgccgtg agcgatgcgg tggccaatta
    16381 aggccacgct gtcatttaaa ttccgttttt ccagttcaaa tgcaattgcc ttcaatgcac
    16441 cttcgtagct gtggtgagcc agcggtgctg gctctccccc atttacggat aagaatgcat
    16501 tttccgagtt aataccgtcg gcaatacctg acattaatac ttcacaatcg ctggcatcga
    16561 gtacggaaaa cttaatcgaa gacgaaccac agttaataac caaaacaacc ggaaattcat
    16621 tcatctcttt tctcatcctg agttacggat taaaacagtt tgtatacgat gttcaggatg
    16681 gtcagcagac caatcacggt aacaaacacg ttatccagac gaccacggta tttcgccaga
    16741 gacggcgctt tacggatagc atacatcggc aacaggcaca gcagggatgc gataatcggt
    16801 gcgcccatgg cttcaatcag gtcgaggatg ttcgggttgg cgtaggcaac aacccaggtg
    16861 gagcccatga tgaagatcat gctgagagta ttcagtttac ccagcgacac tttggttttg
    16921 tcacctttat aaccgaactt cagaatcaga ccattcaagc cttccagcgt ccccagatag
    16981 tgaccgaaga aagatttgaa gatagccacg agtgcgatga tggaagccgc atattccagt
    17041 gtaatcgcga acgttgtttt cgtaccggtc atggacgcaa agtggttagc cagataagaa
    17101 agcactggaa tattctgcgc tttggcttcc gccatgttgg ccggagacag agtaaacagg
    17161 cagctaaagg caaagaacat caccactgca accatcagca tgctggcacg agaaatgatt
    17221 tgggaacatt tacgttcggt gaagtcgcga ccgaagtctt tctcatactc ttcacgttta
    17281 gaaaccacga aggaagagac gattggcgag aagttaaagg agaaaaccat gatggaaatc
    17341 cccagccaga cagtgatcag gataccgtca tgaccggtta acgacagcga accgaggtca
    17401 acctggtcga taactgcaga gttccagtaa gggatcagcg acagagaaat cagcaccagg
    17461 ctggcgataa acggccatac caggtagctc atcactttaa ccatcagatc cttaccaaac
    17521 cagatgacga aagccatcag cagcaacagg aacagcgcca caaagccgcg attcagcggt
    17581 gcaaatccga gctggttttc ccagaacgtc ataaaggtat tggtaatagt aacgccataa
    17641 atccacagca gtgggcaaat cgcgaagaag tacaggaacg tgataaccac gccgccagtt
    17701 ttaccaaaat gctcttccac cgtttccgta atgttgccgg aagggttaga gccagaaaga
    17761 cacagacgcg ccagcgcccg gtggcaataa aatgcgatgg ggtatgccaa taccaacatc
    17821 agaagaatcg ggatcagtcc gccaaaacct gcgcggatag ggaagaacag caccccggcg
    17881 ccgattgccg taccaaacaa gcctaacgtc catgtggtat ctgatttacg ccaggacgat
    17941 tgttttgtct ggctggatac aatgctatct gaagtactca tatcctatcc tcaacgaatt
    18001 aattaagcgt caacgaaacc ggtgatttga gagacgcgag aaagatcgat attgccgccg
    18061 gaaataatac tgacggtttt tctattttga atatattggt ctaatttacc gcttaataat
    18121 gcagcacatg ccagagcgcc tgcgccttcg gtgacgactt tattgcgctg aattaaggca
    18181 atcatactgt ttctgatttc gtcttcgctg accagcacga tgtcatcgac taattcacga
    18241 acgatttcgt aagttaaatt acccgggcgg gagacatcac aaccatccgc cagggtgccg
    18301 gtagttcggt gcgtggttat ttctccggag tggaaagaag ccgccatgcc gtgaacgttt
    18361 tctgactgta cgccaataac acgaatggtc gggttaatag atttaattgc caccgcaata
    18421 ccagcaatta aaccaccacc accaattggc acaatcacgt tatcgacatc atagagatct
    18481 tccataattt ccagaccaat cgttccctgg ccagcaatca ctttcggatc atcgtaaggt
    18541 gggataaaaa tacgaccttc catttcgaca atttcgctca ctttagcgat agtgtcgttg
    18601 aagttatcac catgcagaac gacttctgcg gagtagtcgc acgttgccgc tactttggat
    18661 tttggcgcac cttttggcat caccacttta ccgtcgatac ccagcatcgc gcaggagagc
    18721 gaaacccctt gcgcatggtt gcccgcagaa caggccacca cgcctttgcg tttttccgca
    18781 tcggtcagtg aacttaattt attaaatgcg ccacgaattt taaatgaacc cgtacgctgc
    18841 atattttcaa acttcaggaa tatttcacct ttgcaacgtt cactaaaata gttggagcga
    18901 ggcatgcctg ttttataaat tcgcccagcc agtcgttgtt tcgcttcaat aatatcatca
    18961 atagcaaccg gcagatcgta tgtaatatgc attataaaac ctcttcgcct gaattaaata
    19021 tgtaggtaac cgtaaccgac acctgcaaaa cagacaggtg gattatttat gtttaaataa
    19081 aaacaaacaa ctaaccaact tctattaatt gccttcgtct acacccatta taagatgaat
    19141 actctttggc taattccacc aaaaccgatg ctgctttttt aatacgataa tttttcgacc
    19201 ataccgcggc atattgtgcc acaggtaatg tttcttcaac cggaatagta ataaattgat
    19261 tagaaccaaa aggtgacgtc atatcacaag gaattacagt taagaaatca gcattgagaa
    19321 caagattata aattgtcacg actgagtcgg ttttaacgat gttttcaata ctgatgccat
    19381 ttctttgtaa cgtagtaagc agttcgctgt agtaccccat attagtttgt ggcaacaccc
    19441 actgttcgtt cttcaacgac tccagcgtgg tggtgccggt gcatgttcgg gacttactgg
    19501 ctaccagcac aaactcggac tcgaacagcg gctcaacatg taaatcctga agcttcattt
    19561 ctgcacttaa cgtaccaatc gcaaaatcca gtcgaccgtc gcggattgcc ggtaagaacg
    19621 aagacagttg cgcttcatac atagaaacct gcgctttcgg gaacacctct ttgaacttgt
    19681 tgatcatccc tgacataaaa gtaaaaccaa tcaatgaagg aaaaccaaat gagacttcca
    19741 ccaccgcttc agaagacata ccgcttatct cattaaccat atttttcatt tcacgggtaa
    19801 tggattcgga acgggagagt aacaattgac cggcaggtgt taatgttaca ccggtatttt
    19861 tccgcaccac taattccaca ccaaaataat cttcaatatc gttaatgatt ttactgacgg
    19921 ccggttgagt taaccctaat tcttttgcag ccgagccgat agagccactt ctaatgactt
    19981 cctgaaagac taccaggtgc tgcgttttcg gaagaagaat agtgctcata acgacctacg
    20041 ttaattacct cattgacggc atgaagtgta tcaaaatgaa ataaacagga tatgtgcgac
    20101 cactcacaaa ttaactttca ttactttcca aggtatcgtt attacaatta aacatcttta
    20161 aatatcaaca agttaaagca taaaaatcag cataaaccct gatttttatc aaaaaaaaat
    20221 gacggggata accatatttt atggcgataa catcattcgt tatgtggtta atacaaaaaa
    20281 ggggctgaga ccatatttca agcaattacc ggataattac caggcaaaat ttgagttaaa
    20341 tttaatgagc aagttctcaa attttattat aaataaacca ttttcagcga taaatactgc
    20401 ggcacgtcac attttttcac gctatttgtt ggagaacaaa catttatttt atcaatattt
    20461 taaaatttcg aatacaggta ttgatcatct cgaacaattg attaacgtca actttttctc
    20521 ttctgacagg acgtcatttt gtgaatgcaa tcgttttcca taaattcttc tcccctcata
    20581 ggcgacgaat agcattttgt gttgaggatc acaaaacgaa taattgctgg tcgccgcgat
    20641 aaggtcagac gaagtcaaca agggggattt tcactgtgct ttacatcagc gtaggacaca
    20701 gaatgttgct tcgtttactg taacgccggg taaatgagcg ttttttgata gtgcgtaagt
    20761 gtcgtgcgag gaaaataagc atctattatt gtttgtcatg acgacaaggc aggcatcaat
    20821 gaaaggcttc ccaattgcac atatttttca cccttcaatc ccgccaatgc acgcagtggt
    20881 taacaatcac aatagaaata ttgattattg gacggtaaaa agaaagtttg cagaaattgt
    20941 ctccaccaat gacgttaata aaatttacag tataagtaat gaactccgga gagtattatc
    21001 tgcaattact gcattgaatt tctatcaagg cgatgttcct tctgtcatga ttccgaatcc
    21061 aaccggaaaa tatgagtcca ttcatcatag atatttctac aggagaacat gatgattata
    21121 tcatacaaac attagatgta ggcacttttg caccttttgg tgaacaatgt acttgctcag
    21181 ccgtcaataa aaaagagctg gaatgtatta aggagacgat ttctaagtat tgcgcaaagt
    21241 tcacccaaaa agaagccatc ttaacacccc ctaggcactt taataaaacc tgcattactt
    21301 cagattgttg gcaaattctc tttttttcac ccgatcattt taataatgat ttttattgat
    21361 tatttataaa ggataaaata atgttccctg tttcatcaat tggtaatgat attagtagtg
    21421 atttagtccg tcgaaaaatg aatgaccttc ctgaaagccc cataggaaat aacctcgaag
    21481 cactagcacc aggtatagaa aaactaaaac agacctctat tcaaatggtc actttactta
    21541 acgcgttaca accaagtgga aaatgcatta tcactggtga ttttcaaaaa gaattagcgt
    21601 acttacaaaa tgtaattctt tataatgact cgtctcttcg tctggatttt ttaggttata
    21661 acacccaaat tattcaacga tcggacaata cctgcgaact taccattaat gaaccgttaa
    21721 aaaactagga aatatctaca ggcaatatca atgttaattg cccattaaaa gatatttaca
    21781 atgaaatcag gaggttgaac gtaatcttta gttgtgggac tggagatata gttgatctat
    21841 cctctctgga cttacgtaat gtcgatttag attattatga tttcacagat aaacatatgg
    21901 ctaatactat tctaaatcat tttaaattgg attcaacaaa ttttactaat gccaacatgt
    21961 ttcaggttaa ttttgttagt tcaacacaaa acgccacaat ctcctgggat tatttactaa
    22021 aaataacgcc tgttttaata agcattagcg atatgtattc tgaagaaaaa atcaagtttg
    22081 tcgaaagttg tttaaatgag cttggagaca ttaccgaaga acaattaaaa attatgagat
    22141 ttgcaattat aaaatctata ccaagggcaa ctcttacaga taaattagaa aatgaattaa
    22201 caaaagaaat atataaaagc tcatcgaaaa tcaacaattg cttgaacaga attaaattac
    22261 cagagatgaa agaattctca tcaaaaaaaa catatgatta catcgataca atcattgaag
    22321 attatgaaaa tatcaaagaa aatgcttatc tggtcgtccc ccaaattaat tatactatgg
    22381 atttaaacat agaagactct agctcagaag agttaatacc ctcgagaaag acgaaaattc
    22441 tccggacaat ggctttgagg tcggggaata taacacatat gaagcatata actcagagaa
    22501 tcaatatttt accagagagg actatacgta taattacgac cttttaaatg caatatagac
    22561 ttaataactt cttacaaagt ctattattaa taccgtagat attcatctta ccctgtgcac
    22621 caaaagcaaa aacttatcga cgcgggtttg aaactgatgg caaccgcaaa aatgcctgat
    22681 gcgctacgct tatcaggcct acgccatctc tgcaatatat tgaatttgcg tgcttttgta
    22741 ggcaggataa ggcgttcacg cagcatccgg catgaataaa gcgcactcaa caatctgaaa
    22801 cccgccggaa cggtttatta cgtacatcag gtgaaactga ccgataagcc gctttctttt
    22861 gggtatagtg tcgtggacag tcattcatct ttctgcccct ccaaaagcaa aaacccgccg
    22921 aagcgggttt ttacgtatat caggtgaaac tgaccgataa gccgctttct tttgggtata
    22981 gcgtcgtgga cagtcattca tctttctgcc cctccaaaag caaaaacccg ccgaagcggg
    23041 tttttacgta catcaggtga aactgaccga taagccgctt tcttttgggt atagcgtcgt
    23101 ggacagtcat tcatctttct gcccctccaa aagcaaaaac ccgccgaagc gggtttttac
    23161 gtaaaacagg tgaaactgac cgataagccg ggttctgtcg tggacagtca ttcatctagg
    23221 ccagcaatcg ctcactggct caagcagcct acccgggttc agtacgggcc gtaccttatg
    23281 aacccctatt tggccttgct ccgggtggag tttaccgtgc cacggactgt taccagccgc
    23341 gcggtgcgct cttaccgcac cctttcaccc ttacctgatc ccgcttgcac gggccatcgg
    23401 cggtttgctc tctgttgcac tggtcgtggg tttccccccc aggcgttacc tggcaccctg
    23461 ccctatggag cccggacttt cctcccctcc acccgtctcc cccgaagagg acgacgacga
    23521 agcggcgact gtctggtcag cttcggcgcg cagtatagag ggtttgcgcg cccttgtcac
    23581 cccgtgttgc gcattccaat cgccagtgtc gcggcaatat tacgtgaagc acggcagata
    23641 ttgtcataag ccccgcggaa tgcttcgtcc aacgtaccta tgctggtcaa tacgctgaag
    23701 accgcatcaa tgccatgctg atgtacaacg ccaacatcat cggtcaggct acccgcaatg
    23761 ccaatcaccg gtttatggta cttcttcgcc acgtttgcga cgccaatcgg taccttgccg
    23821 tgaatactct ggctgtcaat acgcccttca ccggtgatca ccagcgtaca atcgtgaata
    23881 tgttcctcca gattcagcgc cgtagtgacg atttcaatac cacttttcag ttccgcacca
    23941 agaaacgcca tcagcgccgc acccataccg cccgcagctc ctgcgccggg gacatctttt
    24001 acatcaacat gcagcgcttt tttaatgaca tcggcatagt gagagaggtt attgtccagc
    24061 tcaacaatca tcgcttcact ggctcccttt tgtgggccaa agatgcgcga tgcgccgtta
    24121 tcgcccacca gcggattggt gacatcacaa gcgacgcgaa tgacgcaatc ttttaagcgc
    24181 ggatcgaggc cggaaatatc aatatcattc agagtattaa gactaccgcc gccaaaacca
    24241 atttcattgc cgttggcgtc gcataatttc gcccccagcg cctgtaccat gcctgcgccg
    24301 ccatcatttg tagcgctacc gccaatgccg ataataatgt ttgtcgcgcc gctctccagc
    24361 gcctgcagga ttaactcgcc tgtgccgcgt gaagtggtca cgagtggatc gcgtttttcc
    24421 gcaggtacca gctccagccc actggccgcc gccatttcaa taaacgcggt tttgccatcg
    24481 ccggagatcc cccaactggc attcactttc tcgcccagcg gccctgtaac ccaggcgtga
    24541 cgttcagaac cctgggtggc tgcaatcatc gcttccaccg ttccttcgcc accgtcggca
    24601 accggaatag aaacgtactg tgcatcagga aaaatttccc gaaatccttt ttctatcgcc
    24661 tgcgcaacct cgctggcaga taaactttct ttataagagt ctggggcgat tacgattttc
    24721 atacctatgc ctgttaccac atgacgccgg agggcgtttc tcttattcgg cctggattcc
    24781 aggcccggat tgcaatacgc catccgggca cgacgtcatt aacgagtaac ttcgactttc
    24841 gccagttttt cgtagtagca caccagggcg ctatgatccg ccgttcctaa accatctgct
    24901 cgcagtgcct gcatcatctc cataaccgca gctgtgagcg gcagttgcgc gccgacgccg
    24961 tgggaagtat ccagcgcatt tgccagatcc ttaatatgca gatcaatacg gaagcccggc
    25021 ttgaagttgc ggtccatcac catcggcgct ttggcatcca gcacggtact gcccgccagt
    25081 ccaccgcgaa ttgcctgata caccaggtcc gggttaacgc ccgctttagt tgccagcgtt
    25141 aacgcttctg acatcgcggc aatattcagc gccacaatga cctgattcgc cagtttggtg
    25201 acgttacctg caccaattgc cccggtatgc accacggaac ccgccattgc tttcatcaaa
    25261 tcatagtatt tgtcgaaaat agccttgtcg ccgcccacca tcactgacag cgtaccgtcg
    25321 atggctttcg gttcaccgcc gctcaccgga gcatccagca tatcaatgcc tttcgctttc
    25381 agcgcttcgc tgatttcacg gcttgccagc ggtgcgatag aactcatatc gatcaatacc
    25441 gtacctggct tcgcgccttc aataatgcca ttctcaccca gcgccacctc tttcacatga
    25501 ggggagtttg gcagcatggt tatgatgacg tcgcactgtt cagcgatcgc tttagccgta
    25561 gaggctgttt ctgcacctgc agcaatcacg tcagcaatag cttctgggtt acggtcagca
    25621 accaccagcg agtaacctgc tttcagaagg tttttactca ttggtttacc cataatcccc
    25681 aggccaataa aaccaacttt catagtcata tcaatcatct ctcttgttgc ggtggtggtt
    25741 attttttaaa ggtatcagcc agtttatgag tggcagagcg gaagacgccg agatcgctgc
    25801 cgacagccac aaacgtcgcg ccccattcca gataacgacg cgcatcggct tcgaccggcg
    25861 cgaggatacc gctgggtttg ccgtgcgcgc tggcacggtt aaaaatgtgc tgaattgctt
    25921 tttgtacatc cgggtgtgat gcattgccga gatggcctaa tgccgcggcc agatcgctgg
    25981 ggccgacgaa gatgccgtct acgccttcgg tagcggcaat ggcatcgacg ttatctacgc
    26041 cctgctgact ttctatctgg accagaatag tgatgttctt gttcgactga gcgaaataat
    26101 ccgccacggt gccaaacata ttggcgcggt gagaaacgga gacgccgcga atgccttccg
    26161 gtgggtaacg ggttgatgcc accgcctgct ctgcttcctc ttttgtttct acaaaaggaa
    26221 tcaggaagtt atagaaaccg atatccagaa gacgcttaat aatttccggc tcgttggtcg
    26281 gcactcacac tactggcgcg ctggcgctgc ctttcaaggc cattaactgc ggaataaacg
    26341 tggagatatc gtttggcgca tgttcgccat ccagcaccag ccagtcaaac ccagccaaac
    26401 caagaacttc agtgctaatc gggttagaga gtgctgacca gcaaccaatt tgtacctgtt
    26461 tcgcagccag tgcggctttg aatttattcg ggaaaacatc gttattcatc gcttatacct
    26521 ttgcttattt ctgcaattcc atacgtttaa tgtcgccaac tacgaagagg tagcagacca
    26581 tcgccatcag cgctgaacat cccacgaaaa ccagtgctgc attgaaggag tgcagttcac
    26641 ttaccaggta gccaatcacc agtggagtga caatggaggc aacattgcca aagacgttaa
    26701 agacgccgcc acagaggcca acaatctctt tcggcgcggt gtcagaaatc accggccagc
    26761 ccagagcacc aaatcctttg ccaaaaaaag ccagcgccat cagcatgacc accagcgtgg
    26821 tgttgttggt gtagttacat aagatgatgg tggaagccag caacattccc agcacaatcg
    26881 gtagcttacg tgccagggtc agggataaac cgcgtttgat cagataatcc gagaagacac
    26941 ctcccagcac gccgcccgca aaaccacaca gtgccggaat cgaggcgacc agacccactt
    27001 tcagaatcga catgcctttt tcctgcacca gataaatcgg gaaccaggtg aggaagaacc
    27061 aggtgatggt gttgataaaa tattgtccga aaaatacgcc caacatcatg cggttagaga
    27121 gcaattgctt gatgtaatgc agtttgggac cgcttgctgc cgcgctgccc ggctttttgt
    27181 ggtccatatc gaccaccgcg ccattttcag agataaactt cagctcttcc tcagacatac
    27241 gtgggtgatc tgtcgggtta tgaatcaact tgatccacag cgccgtcagc acaaaacaaa
    27301 tcacccccat aacggtaaag acgtgctccc atccccaggc gaaagtcagc cagccaagca
    27361 gaggcgaaaa gagcgccagc gagaaatatt gtgccgagtt aaagatggcg gaggcagtac
    27421 cacgttcttt cgtcgggaac caggcggcga caatacgggc gttcgccggg aaggatggag
    27481 cttccgagaa tcccagcata aagcgcataa agaacatgga gatccctgcc caagccagcg
    27541 ggaacatatc aacaaagcct tgcaggaagg tgaacagcga ccagaaaaag aggctgtagg
    27601 tgtaaacttt tttcgagcca aacttatcaa gcaaccagcc gccggggatt tgcatcagca
    27661 agtaggccca gccaaaagcg gagaagatgt aacccatcga aaccgcactt aactgcaact
    27721 cttttgccac ttcggtacca gcaatagaca gcgttgcacg atcggcgtag ttaacggcgg
    27781 taacaataaa aataatcagt aatattaaat agcgggtatg cacacctttc cttttttcgt
    27841 caacggtgtc cagaatcatt ttatttacct cgggtactta tgctgatttt tattattatg
    27901 gggaaggtgt tatttataag tttcatttat gccgtaacga caatgaactc gggaattagt
    27961 ataagcagcg cgagaataat aatcattgtg caaatgctaa tttaattaat actttttaaa
    28021 tattattttg agcatatgca cataaggttg cgcgctaaag cacagatttg cgctttacct
    28081 tgccgggcgg cactgcaatc cctaaaatga ttgacattga tcacatttct gcgtttaaac
    28141 tcctgacatt cttatttcac ccaatgaaat catttatttt taaatgagac caggtcctca
    28201 ttttaataac ccctggctgg agaatattgc acaatggcca acatcgaaat cagacaagaa
    28261 acgccaactg cgttttatat aaaagttcac gacacagata atgtggcaat tattgttaat
    28321 gataatggcc tgaaagcagg aacgcgtttt ccggatgggc tggaattaat tgaacatatt
    28381 ccccaggggc ataaagtcgc attgctggac attccggcta atggtgaaat tattcgttat
    28441 ggcgaagtga ttggttacgc cgtgcgtgca atcccacgcg gaagctggat cgacgaatca
    28501 atggttgtac taccggaagc gccgccgtta cacacgctgc cactggcaac caaagtcccg
    28561 gaacccttac cgccgctgga aggatacacc tttgagggct atcgcaatgc cgatggcagc
    28621 gtgggcacca aaaacctgct cggcatcacc accagcgtcc actgtgtggc aggtgtggtg
    28681 gactatgtag taaaaatcat tgaacgcgat ctgctaccga aatacccgaa cgtcgatggc
    28741 gtggtggggc tgaatcattt gtacggttgt ggcgtggcga ttaacgcacc ggcagcagtt
    28801 gtacctattc gtaccattca caatatttcg ctgaacccta actttggcgg cgaagtaatg
    28861 gtgattggcc tgggttgtga aaagttgcag cctgagcgcc tgctgactgg aacggatgat
    28921 gtgcaagcta ttccagtaga aagcgccagc attgtcagtt tgcaggatga aaagcatgtc
    28981 ggttttcagt ccatggtcga ggatattttg caggtcgccg aacgccatct acaaaaactg
    29041 aatcaacgtc agcgagaaac ctgtccggct tcagaactgg ttgtcggcat gcattgcggt
    29101 ggcagcgatg cgttttctgg cgtaacggca aacccggcgg ttggctatgc gtctgatcta
    29161 ctggtgcgct gcggcgcaac ggtgatgttt tcagaagtca cggaagtgcg tgacgcgatc
    29221 catctgctga caccacgcgc agtgaacgaa gaggtcggca aacggctgct ggaggagatg
    29281 gagtggtacg ataactatct caatatggga aaaaccgacc gcagcgccaa cccttcgccg
    29341 ggcaacaaga aaggcggtct ggcaaacgtg gtagagaaag cgctcggctc cattgctaaa
    29401 tcgggcaaaa gtgcaattgt tgaagtgctg tcgcccggtc aacgcccgac taaacgcgga
    29461 ttaatttacg ccgcgacgcc agccagcgat tttgtctgtg gcacgcaaca ggtggcttcg
    29521 ggtatcaccg tgcaagtgtt tacgaccggt cgtggtacgc cgtacggcct gatggcggta
    29581 cccgtcatta aaatggcacc ccgcaccgag ctggcgaacc gctggtttga tttaatggat
    29641 attaatgcgg gcaccatcgc taccggcgaa gaaactattg aagaggaggg ctggaagttg
    29701 ttccacttta ttctcgacgt cgccagcggg aagaagaaaa ccttctcgga tcaatgggga
    29761 ctgcataacc agctggcggt gtttaaccca gcaccggtga cctgataatt caattcgtat
    29821 tatgcccgat agcacacgtt tatcgggcat attgagtcac agcgaagacg cgtgatcccc
    29881 gaccagaatc acttcaaccc cagcctttcg cagtccttcc agactatccg caggaatgcc
    29941 ttcatcaaca atgatcatgt cgatacgttg agtatcaatg atcttatgta aactggaacg
    30001 attgaattta ctggaatcgg tgaccacgat gatccgttcc gcaacttcgc acatccgacg
    30061 gcttaaacgg gcttcatctt cattatgtgt gctgacgccg cgctccagat cgatcgcatc
    30121 tacaccaaga aacagcatat cgaagtggta attttgcagc gattgctcag cctgatcgcc
    30181 gtaaaaagat tgcgactgac ggcgcaaatg cccgccggtc atcagcagct caacgccttc
    30241 cgcttccagc aacgcattag ccacgttcat accgttggtc atcgcaatta cgtcagtgta
    30301 cttgcgcatc agacgagcaa tctcaaaagt ggtggtcccg gaatcgagga tcacccgatg
    30361 acctggctga atcaactcaa cggcagcttt cgcaacgctg cgtttcatcg cggtgttcag
    30421 tgcgctttta tcttccactg atggctcgac tgacggcgtc gtgctatcgc agatcaacgc
    30481 gccaccatag gcacgcacag cgatcccctg cttttccaga aacgccagat cattgcggat
    30541 cgtcacagta gatacgccat acaatgccga cagatcgtta acctgcacac tcccttgctg
    30601 tcgcagacgc tgaatgatct gttctcgtcg ctcgctggtg cctgtcactc gcttctcacc
    30661 tgaagcgtcg gtattactca tagtaagtcc tttcgtaaaa ctttcgtttc atttcgtttt
    30721 gcctattaac gcctttctat taagcaaatg caagcccacc ttgcccattg gcgcaagcta
    30781 ctctcgtttc agtgactttc attacgtttc ttttgtgaat cagatcagaa aactattatc
    30841 tttcgtttta tttttatctc accatgacgc agtatcaatt gaaacaaaac gaaagatcaa
    30901 tatcgcagta atctgaactg gagaggaaag tgaaacatct gacagaaatg gtgagacagc
    30961 acaaagcggg caaaacaaat gcaatttatg ccgtttgttc cgcacatccg ctggtgctgg
    31021 aggctgcaat ccgctacgcc agtgcaaacc aaacgccgtt actgattgaa gcaacctcca
    31081 atcaggtaga ccagttcggc ggttataccg gaatgacgcc cgccgatttt cgcggctttg
    31141 tttgtcagct cgccgactcg ttgaatttcc cgcaggatgc gctgattctg ggtggtgacc
    31201 atctggggcc aaaccgctgg caaaacctgc cagccgctca ggcaatggcc aatgccgatg
    31261 atttgattaa aagctacgtt gcggcaggat tcaaaaaaat ccaccttgat tgcagcatgt
    31321 cctgtcagga cgatccaatt cccttaactg atgacatcgt ggctgaacgc gccgcccgtc
    31381 tggcgaaagt ggcggaagaa acctgtcgcg aacactttgg cgaagccgat ctggagtatg
    31441 tcattggtac cgaagtgccg gtacctggcg gcgcgcatga aaccttaagc gagctggcgg
    31501 tcaccacgcc ggatgccgcc cgcgccacgc tggaagccca tcgtcacgcc tttgaaaagc
    31561 aaggtttgaa tgccatctgg ccacgcatta ttgccctggt ggttcaaccc ggcgtcgaat
    31621 tcgatcacac caacgttatt gattatcagc ccgccaaagc gagcgcctta agccagatgg
    31681 tcgaaaacta cgaaacgctg attttcgaag cgcactctac cgattatcaa acgccgcaat
    31741 ctctgcgcca gctggtgatt gaccactttg ccattctgaa agttggcccg gcgctgacct
    31801 tcgccctgcg tgaagccctt ttttctctgg cggcgattga agaagaactg gtaccagcga
    31861 aagcctgttc cggtctgcgt caggtgctgg aaaacgtgat gctcgaccgc ccggaatact
    31921 ggcaaagcca ctaccacggt gacggcaacg cgcgtcgtct ggcgcgtggt tacagctact
    31981 cagatcgcgt gcgctattac tggccggaca gccagattga tgacgctttc gctcatctgg
    32041 ttcgtaatct ggcggattca ccaattccgc taccgctcat cagccagtat ctgccgctgc
    32101 agtacgtgaa agttcgctcc ggcgagctgc aaccgacgcc acgggaactc attataaacc
    32161 atattcagga catcctggcg cagtaccaca cagcctgtga aggccaataa gcaaaacaaa
    32221 gaggaacatg ctatgccaaa tattgtttta agccggattg atgaacgctt gattcacggt
    32281 caggtcggcg ttcagtgggt cggatttgcg ggggcaaatc tggtgctggt agccaacgat
    32341 gaggttgccg aagatccggt acaacaaaac ctgatggaaa tggtactggc agaagggatc
    32401 gccgtacgtt tctggacgct gcaaaaagtt atcgacaaca ttcatcgcgc cgccgatcga
    32461 cagaaaatcc tgctggtttg taaaacaccc gccgatttcc tgacgctggt gaaaggtggc
    32521 gttccggtga atcgcattaa cgttggcaat atgcactacg ccaatggcaa acaacaaatc
    32581 gccaaaacgg tttctgtgga tgcgggcgat atcgcagcat ttaacgacct gaaagccgct
    32641 ggggtggaat gcttcgttca gggcgtcccg acagagcctg ctgtggacct ctttaaatta
    32701 ctttgaggga ttcatcatgg aaatcagcct gttgcaggca tttgcgttgg gcattatcgc
    32761 ctttatcgct ggcctagata tgtttaacgg cttaacccat atgcaccgcc cggtggtcct
    32821 cggcccgttg gtcgggctgg tacttggcga tctgcatacc ggaattttaa ccggcgatac
    32881 gctggaacta gtgtggatgg ggctggcccc gctggcgggc gcacagccgc ctaacgtgat
    32941 tatcggtact atcgtcggca cggcgtttgc cattactact ggcgtgaaac ccgatgtcgc
    33001 ggtcggtgtc gccgtacctt tcgctgtcgc agtacagatg gggattacct tcctgttctc
    33061 ggtgatgtct ggcgtgatgt ctcgctgcga ccggatggcg gagaacgccg atactcgcgg
    33121 cattgaacgc gtgaactatc tggcactgct ggcactcggc actttctatt ttctctgcgc
    33181 tttcctgcct atctacttcg gcgcggaaca tgcaaaaacc atcattgatg tcctgccgca
    33241 acgattaatc gacggcctcg gcgtcgcggg cggcatcatg ccagcaatcg gttttgccgt
    33301 gctgctgaaa atcatgatga aaaacgtcta cataccctac ttcatcctgg gctttgttgc
    33361 cgcaacctgg ctcaagttac cggtgctggc tattgctgcc gccgcgctgg cgatggcgct
    33421 gatcgatctg ctgcgtaaat ctcctgaacc gactcaacct gcggcacaga aagaggaatt
    33481 tgaagatggc atctaatcaa accaccctgc cgaacgtcag cgaaaatgaa gaaacactgc
    33541 tgactggcgt caatgaaaac gtgtatgaag atcggagcat tgccgcggag ctgacgaaaa
    33601 aagatatcaa ccgcgtcgcc tggcgttcca tgctgttaca agcgtctttt aactacgaac
    33661 gtatgcaggc ttctggctgg ttgtacggtc tgctgcctgc tctgaaaaag atccacacta
    33721 ataaacgcga cctggcgcgc gccatgaagg ggcatatggg tttcttcaat acccatccgt
    33781 ttctggtgac gtttgttatc ggcattatcc ttgcgatgga gcgttctaag caggacgtta
    33841 acagtattca gagcaccaaa attgccgtcg gtgcgccgct tggcgggatt ggcgatgcga
    33901 tgttctggct aacgctactg ccgatttgtg gcgggatagg cgcaagcctc gcgctacaag
    33961 gctctattct tggcgcagtc gtctttattg tgctgttcaa cgtggtacac ttggggctgc
    34021 gttttggtct ggcgcattat gcttaccgca tgggcgtggc ggcgattccc cttattaaag
    34081 cgaataccaa aaaagtcggc catgctgcgt caatcgttgg aatgacggta atcggcgcac
    34141 tggtggcaac ctatgttcgt ttaagcacca cgctggaaat cacggcgggc gacgcagtag
    34201 ttaagttaca ggctgatgtt atcgacaaac tgatgcctgc cttcttaccg ctggtctaca
    34261 ccctgaccat gttctggctg gtacgccgcg gctagagtcc gctgcgcctg attgcggtta
    34321 ccgtggttct agggatcgtc ggtaaattct gccatttcct ttaaatacaa tgaggtatta
    34381 gatgttaagt attattttga cagggcatgg cgggtttgcc agtggcatgg aaaaagcaat
    34441 gaagcaaatc ctcggcgatc aatcgcagtt tattgccatc gattttcccg aaacctcaag
    34501 cgccgcgctg cttacatcgc agcttgaaga ggccatcgcc caactggatt gtgaagacgg
    34561 cattgttttt ctgacggatt tgctgggcgg cacgccgttt cgcgtagctt caacgctggc
    34621 gatgcaaaaa ccgggctgtg aagtgattac cggcaccaat ttgcaactgc tactggagat
    34681 ggtgctggag cgcgaagggt taagcggtga agaattccgt gtacaggcgc tggagtgtgg
    34741 acaccgaggg ctgaccagtc tggtggacga gttaggccgc tgccatgaag aatgtccggt
    34801 cgaggaagga atatgacaca cgttctgcgc gccagaaggc tgctgactga agagggatgg
    34861 ttcgatgacc atcagttgcg tgttgctgac ggtgtcatcg cagcaatcga accgattcca
    34921 gtgggcgtga ctgaacgcga tgcggaacgg ctctgccccg cttacatcga cacccatgta
    34981 cacggtggtg cgggcgttga tgtaatggat gacgcgccgg atgtactcga caagctggca
    35041 atgcacaagg cacgcgaagg tgtcggcagt tggttaccga ccaccgtaac cgcgccgctt
    35101 aataccattc atgcggcgct gaaacgtatt gctcaacgtt gccaacgcgg cggacctggt
    35161 gcgcaagtgc tggggagtta tctcgaagga ccgtacttca cgccgcagaa taaaggcgcg
    35221 catccg
//
LOCUS       DAJTRD010000033        34902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTRD010000033 DAJTRD010000000
VERSION     DAJTRD010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.033"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            857..3142
                     /gene="nrdA"
                     /locus_tag="OO315_001813"
     CDS             857..3142
                     /gene="nrdA"
                     /locus_tag="OO315_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5890749.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3376..4506
                     /gene="nrdB"
                     /locus_tag="OO315_001814"
     CDS             3376..4506
                     /gene="nrdB"
                     /locus_tag="OO315_001814"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5890750.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4506..4760
                     /gene="yfaE"
                     /locus_tag="OO315_001815"
     CDS             4506..4760
                     /gene="yfaE"
                     /locus_tag="OO315_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5890751.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4814..5464)
                     /gene="inaA"
                     /locus_tag="OO315_001816"
     CDS             complement(4814..5464)
                     /gene="inaA"
                     /locus_tag="OO315_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5890752.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5643..5885
                     /locus_tag="OO315_001817"
                     /pseudo
     CDS             5643..5885
                     /locus_tag="OO315_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5927..7003)
                     /gene="glpQ"
                     /locus_tag="OO315_001818"
     CDS             complement(5927..7003)
                     /gene="glpQ"
                     /locus_tag="OO315_001818"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5890753.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7008..8366)
                     /gene="glpT"
                     /locus_tag="OO315_001819"
     CDS             complement(7008..8366)
                     /gene="glpT"
                     /locus_tag="OO315_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5890754.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8639..10267
                     /gene="glpA"
                     /locus_tag="OO315_001820"
     CDS             8639..10267
                     /gene="glpA"
                     /locus_tag="OO315_001820"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5890755.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10257..11516
                     /gene="glpB"
                     /locus_tag="OO315_001821"
     CDS             10257..11516
                     /gene="glpB"
                     /locus_tag="OO315_001821"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5890756.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11513..12703
                     /gene="glpC"
                     /locus_tag="OO315_001822"
     CDS             11513..12703
                     /gene="glpC"
                     /locus_tag="OO315_001822"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5890757.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12896..13846
                     /locus_tag="OO315_001823"
     CDS             12896..13846
                     /locus_tag="OO315_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5890758.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13887..14357)
                     /locus_tag="OO315_001824"
     CDS             complement(13887..14357)
                     /locus_tag="OO315_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5890759.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14412..15614)
                     /locus_tag="OO315_001825"
     CDS             complement(14412..15614)
                     /locus_tag="OO315_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5890760.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15701..16243)
                     /locus_tag="OO315_001826"
     CDS             complement(15701..16243)
                     /locus_tag="OO315_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890761.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16522..16947
                     /gene="nudI"
                     /locus_tag="OO315_001827"
     CDS             16522..16947
                     /gene="nudI"
                     /locus_tag="OO315_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5890762.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16986..17588)
                     /locus_tag="OO315_001828"
     CDS             complement(16986..17588)
                     /locus_tag="OO315_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5890763.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQNKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17896..19035
                     /gene="arnB"
                     /locus_tag="OO315_001829"
     CDS             17896..19035
                     /gene="arnB"
                     /locus_tag="OO315_001829"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5890764.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19039..20007
                     /gene="arnC"
                     /locus_tag="OO315_001830"
     CDS             19039..20007
                     /gene="arnC"
                     /locus_tag="OO315_001830"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5890765.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20007..21988
                     /gene="arnA"
                     /locus_tag="OO315_001831"
                     /pseudo
     CDS             20007..21988
                     /gene="arnA"
                     /locus_tag="OO315_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21985..22875
                     /locus_tag="OO315_001832"
     CDS             21985..22875
                     /locus_tag="OO315_001832"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5890766.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22875..24527
                     /gene="arnT"
                     /locus_tag="OO315_001833"
     CDS             22875..24527
                     /gene="arnT"
                     /locus_tag="OO315_001833"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5890767.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24524..24859
                     /gene="arnE"
                     /locus_tag="OO315_001834"
     CDS             24524..24859
                     /gene="arnE"
                     /locus_tag="OO315_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5890768.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24859..25245
                     /gene="arnF"
                     /locus_tag="OO315_001835"
     CDS             24859..25245
                     /gene="arnF"
                     /locus_tag="OO315_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5890769.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25239..25511)
                     /gene="pmrD"
                     /locus_tag="OO315_001836"
     CDS             complement(25239..25511)
                     /gene="pmrD"
                     /locus_tag="OO315_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5890770.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25616..26971)
                     /gene="menE"
                     /locus_tag="OO315_001837"
     CDS             complement(25616..26971)
                     /gene="menE"
                     /locus_tag="OO315_001837"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5890771.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26968..27930)
                     /gene="menC"
                     /locus_tag="OO315_001838"
     CDS             complement(26968..27930)
                     /gene="menC"
                     /locus_tag="OO315_001838"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5890772.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27930..28787)
                     /gene="menB"
                     /locus_tag="OO315_001839"
     CDS             complement(27930..28787)
                     /gene="menB"
                     /locus_tag="OO315_001839"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5890773.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28802..29560)
                     /gene="menH"
                     /locus_tag="OO315_001840"
     CDS             complement(28802..29560)
                     /gene="menH"
                     /locus_tag="OO315_001840"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5890774.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29557..31227)
                     /gene="menD"
                     /locus_tag="OO315_001841"
     CDS             complement(29557..31227)
                     /gene="menD"
                     /locus_tag="OO315_001841"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5890775.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31316..32611)
                     /gene="menF"
                     /locus_tag="OO315_001842"
     CDS             complement(31316..32611)
                     /gene="menF"
                     /locus_tag="OO315_001842"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5890776.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32690..32995)
                     /gene="elaB"
                     /locus_tag="OO315_001843"
     CDS             complement(32690..32995)
                     /gene="elaB"
                     /locus_tag="OO315_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5890777.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33050..33511)
                     /locus_tag="OO315_001844"
     CDS             complement(33050..33511)
                     /locus_tag="OO315_001844"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5890778.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33576..34493
                     /gene="rbn"
                     /locus_tag="OO315_001845"
     CDS             33576..34493
                     /gene="rbn"
                     /locus_tag="OO315_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5890779.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            34684..34872
                     /locus_tag="OO315_001846"
                     /pseudo
     CDS             34684..34872
                     /locus_tag="OO315_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311184.2"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         8536 a         9338 c         8648 g         8380 t
ORIGIN      
        1 gtaatgcctg aaaggctttg cctgctggcg tgacaactcg ctttgatatc atatccctgg
       61 cataaatcaa tggccgccgc gacaccgtta gcagagaaaa gagatgcaat cattgacggg
      121 agtagagata aatactcctt gcgtagaaag ataatccgca tattaatctg accatcattt
      181 tatataagac ggcatattta aacgcattat acaatatggg ttatctttct tttgtaaatt
      241 gtccaacccg atgcaatggc tattttgtta ttatttgatg aataatatca gtgcgtcata
      301 attcaagtta ataaccttaa gggataccag ttatatttaa actaaattaa agtaatgaat
      361 aattttctta taatataagg taaattaaca aaatggcttc gcatttaaca ataaccgaat
      421 agaaaacaac catttcgaca tcaacaatcg cttacattcg cttatatatt gaccacaact
      481 gatacatcag attatgtgat gactcgtgct tagatcaatt tttgcaatca ttagcaaaaa
      541 gattaataaa ccatctatat caatttatcc aacctattct gccgttcaag aaatcgccga
      601 acagttattt ttaacaaatt tttctcttcc cattgacttt cccggacacc ttgtctgacc
      661 taaggtgcgc gaaagccact ttttccttcc tgagttatcc acaaagttat gcacttgcaa
      721 gagggtcatt ttcacactat cttgcagtga atcccaaaca taccccctat atatagtgtt
      781 ctaagcagct tcccttacta caggtagtct gcatgaaact attgcggaaa gaattccaaa
      841 aacaggtacg acatacatga atcagaatct gctggtgaca aagcgcgacg gtagcacaga
      901 gcgcatcaat ctcgacaaaa tccatcgcgt tctggattgg gcggcagaag gactgcataa
      961 cgtttcgatt tcccaggtcg agctgcgctc ccacattcag ttttatgacg gtatcaagac
     1021 ctctgacatc cacgaaacta ttatcaaggc tgccgcagac ctgatctccc gtgatgcgcc
     1081 ggattatcag tatctcgccg cgcgcctggc gatcttccac ctgcgtaaaa aagcctacgg
     1141 ccagtttgag ccgcctgcgc tgtacgacca cgtggtgaaa atggtcgaga tgggcaaata
     1201 cgataatcat ctgctggaag actacacgga agaagagttc aagcagatgg acacgtttat
     1261 cgatcacgac cgtgatatga ccttctctta tgctgccgtt aagcagctgg aaggcaaata
     1321 tctagtacag aaccgcgtga ccggcgaaat ctacgaaagc gcccagttcc tttatattct
     1381 tgttgccgcg tgcctgttct cgaactaccc gcgtgaaacg cgcctgcaat acgtgaagcg
     1441 tttttatgac gcggtttcca catttaaaat ttcgctgccg acgccaatca tgtccggcgt
     1501 gcgtaccccg actcgtcagt tcagctcctg cgtactgatc gagtgcggtg acagcctgga
     1561 ttccatcaac gccacctcca gcgcgattgt gaaatatgtt tcccagcgtg ccgggatcgg
     1621 catcaacgcc ggacgtattc gtgcgctggg cagcccgatt cgcggtggcg aagcgttcca
     1681 taccggctgt atcccgttct acaaacattt ccagacagcg gtgaaatcct gctctcaggg
     1741 cggtgtgcgc ggcggtgcgg caacgctgtt ctacccgatg tggcatctgg aagtggaaag
     1801 cctgctggtg ttgaaaaaca accgtggggt tgaaggcaac cgcgtgcgtc atatggacta
     1861 cggggtacaa atcaacaaac tgatgtatac ccgcctgctg aaaggtgaag atatcaccct
     1921 gttcagcccg tccgacgtac cggggctgta cgacgcgttc ttcgccgatc aggaagagtt
     1981 tgaacgcctg tataccaaat atgagaaaga cgacagcatc cgcaagcagc gtgtgaaagc
     2041 cgttgagctg ttctcgctga tgatgcagga acgtgcgtct accggtcgta tctatattca
     2101 gaacgttgac cactgtaata cccatagtcc gtttgatccg accatcgcgc cagttcgcca
     2161 gtctaacctg tgcctagaaa ttgccctgcc gaccaaaccg ctgaacgacg tcaacgacga
     2221 gaacggtgaa atcgcgctgt gtacgctgtc tgctttcaac ctgggcgcaa ttaatagcct
     2281 ggatgaactg gaagagctgg cgattctggc ggttcgtgca cttgacgcgc tactggatta
     2341 tcaggattac ccgatcccgg ccgccaaacg tggagcgatg gggcgtcgta cgctgggtat
     2401 tggtgtgatt aacttcgctt actatctggc gaagcacggt aaacgctact ccgacggcag
     2461 cgccaacaac ctgacgcata aaaccttcga agcgattcag tattacctgc tgaaagcgtc
     2521 taatgagctg gctaaagagc aaggcgcgtg cccgtggttt aacgaaacca cttacgcgaa
     2581 agggatcctg ccgatcgata cctataagaa agatctggat gccatcgcta atgagccgct
     2641 gcattacgac tgggaagcat tgcgtgaatc aatcaaaacg cacggcctgc gtaactccac
     2701 gctttctgcc ctgatgccgt ccgagacctc ttcgcagatc tctaacgcca ctaacggtat
     2761 tgaaccgccg cgcggttacg tcagcatcaa agcgtcgaaa gacggtattt tgcgccaggt
     2821 ggtgccggac tacgagcacc tgcacgacgc ctatgagctg ctgtgggaaa tgccgggtaa
     2881 cgatggctac ctgcaactgg taggtatcat gcagaaattt atcgatcagt cgatctctgc
     2941 taacaccaac tacgatccgt cacgtttccc gtcaggaaaa gtgccgatgc agcagttgct
     3001 gaaagacctg ctcaccgcct acaaattcgg ggtcaaaacg ctgtattatc agaacacccg
     3061 tgacggcgct gaagacgcgc aggacgatct ggtaccgtca atccaggacg atggctgcga
     3121 aagcggcgca tgtaagatct gatattgaga tgccggatgc ggcgtaaacg ccttatccgg
     3181 cctacggctc ggtttgtagg cctgataaga cgcgccagcg tcgcatcagg ctccgggtgc
     3241 cggatgcagc gtgaacgcct tatccggcct acggctcgga tttgtaggcc tgataagacg
     3301 cgccagcgtc gcatcaggca caggatgcgg cgtaaaatgc cttatccggc attaaactcc
     3361 caacaggaca cactcatggc atataccacc ttttcacaga cgaaaaatga tcagctcaaa
     3421 gaaccgatgt tctttggtca gccggtcaac gtggctcgct acgatcagca aaaatatgac
     3481 atcttcgaaa agctgatcga aaagcagctc tctttcttct ggcgtccgga agaagttgac
     3541 gtctcccgcg accgtataga ttaccaggcg ctgccggagc acgaaaaaca catctttatc
     3601 agcaacctga aatatcagac gctgctggat tccattcagg gtcgtagccc gaacgtggcg
     3661 ctattgccgc ttatttctat tccggaactg gaaacctggg tcgaaacctg ggcgttctca
     3721 gaaacgattc attcccgttc ctatactcat atcattcgta atatcgttaa cgatccgtct
     3781 gttgtgtttg acgatatcgt caccaacgag cagatccaga aacgtgcgga agggatctcc
     3841 agctattacg atgaactgat cgaaatgacc agctactggc accttctggg cgaaggcacc
     3901 cacaccgtta acggtaaaac cgtaaccgtt agcctgcgcg agctgaagaa aaaactgtat
     3961 ctctgcctga tgagcgttaa cgcgctggaa gcgatccgct tctacgtcag ctttgcttgt
     4021 tcctttgcct ttgcagaacg cgaactgatg gaaggtaacg ccaagattat tcgcctgatt
     4081 gcccgcgatg aagccctgca cctgaccggt actcagcata tgctgaatct gctgcgcagc
     4141 ggcgcggatg atccggagat ggcggaaatt gccgaagaat gtaagcagga gtgctatgac
     4201 ctgtttgtcc aggcggcaca gcaagagaaa gactgggcgg attatctgtt ccgtgacggt
     4261 tcgatgattg gtctgaataa agatattctc tgccagtacg ttgaatacat caccaatatc
     4321 cgcatgcagg cagtcggttt ggatctgccg ttccagacgc gctccaaccc gatcccgtgg
     4381 atcaacacct ggctggtgtc tgataacgtg caggttgctc cgcaggaagt ggaagtcagt
     4441 tcttatctgg tcgggcagat tgacgcggaa gtggacaccg acgatttgag taacttccag
     4501 ctctgatggc ccgcgttacc ctgcgcatca ctggcacaca actgctgtgc caggatgaac
     4561 acccttccct tctggcggcg ctggaatccc acaatgtggc ggttgagtac cagtgccgcg
     4621 aaggttactg cggctcctgt cgcacccgac tggtcgcagg tcaggtggac tggattgccg
     4681 aaccgttagc ctttattcag ccgggggaaa ttttgccctg ttgttgccgg gcaaaaggcg
     4741 atattgaaat cgagttgtga tcgggtacta gcgccagata cgacgcttac gcgtattatc
     4801 tggcctacaa cgattacatg gcgtagtaat acgctttcac ctgctcccag tccgctttag
     4861 ggatcggctc cagatatttt tccagctggc ggaagtcatg attaatcgct ttatcgcgac
     4921 gcaagcggcg acgacttttc tccagatcaa gaaaaccagc ttctgcctta ccttctgttt
     4981 tcacatagat atggcgaaca tagcaacagc catgctgacg attaacgcta tgcattttct
     5041 taaacgccag tgccactgct ttcaacatgg cttgccgtac ttcgtcagaa taaggcgata
     5101 ctgcatgccg ggcataccag tcagcaatgc tgatgaaccc cgccatatct tcagtcacca
     5161 gcaacgcgcg ccattcaccc tcaattttca ccgcttcacc aaaaacgatc ttcggcacga
     5221 tgacaccggc ccgttccagt tctttaatta ccgcaacctc acggacaatc gttggtcggc
     5281 cgaacggata acgtactgaa tgaaacagat gatgcgtcat gcgctttaca tacagctttt
     5341 tgccgttgcg ctcgacgcat tgcaccccgc tcataccatt acggcgatag ttaggctctt
     5401 caacccagtc gccctctgtt gcccaccagt gattaaattc gtcgtacttt gctgaaactg
     5461 ccatacccta tcgcctgtca tttttattaa cgacaatgac tataggtggt tacctgagga
     5521 aaatcttaat gaaacgtgtc gtattaatga atttcttttg ccgctacagg tgtgacaccg
     5581 cttttgccct tcatttgcag cgcatagatt ccaccagcca ctaccactaa tagtgcgccc
     5641 agcagataaa atgtgaattt aatcagtcgg atacagcgat gaattttatt cgacagggat
     5701 taggcatcgc cttacaacca gagttaacgc tgaaaagcat tgcaggtgaa ttgtgttccg
     5761 ttcctcacga accaaccttc tatcgacaga tttcgttgct gactaaagaa aagccggtag
     5821 aaggcagtcc actgttttta ctacaaatgt gcatggaaca attagtggcg attggaaaaa
     5881 tttgatatga gacaggatgg cgcatgaacg ccatcctgca gtaacattac tctttattaa
     5941 gaaattttac tgccttatca gggaaatcag taaacagccc atttacaccc gctttgttat
     6001 acagagcatc atataactga ttcacatcag gagtgtattc aggcagttta tctgaccgca
     6061 cggtataagg atgcactacc agtttattct gctgggcatc ttgcaccatg ccagtgagtt
     6121 tgatattacc cggctgcgat gtctcctcaa tcaacatatg gtaatccgga ccaataccat
     6181 ctgcatattc cgccacctgt ttcatggctc ccggcttaaa catccagtcg tagttgtaat
     6241 taacccagct tccatccggc tgtttctgct gcgtttcatt ccagtcggta taggcaatca
     6301 gctgtaccag attgagctcc atgcccattt tgggttccag ctcattctta atacgcttca
     6361 gctcatcagc atcaaaacat tgcaaataaa ctttatcgtc tttaccggtg taaccatatt
     6421 tcttcagcac ttccagcgtt tttgccgcaa tatccttccc ttcctgatga tggaaccacg
     6481 gcgctttgat ttctggatag ataccgatat ttttcccggt agagtgattt aacccctgaa
     6541 caaattcaat ctcttcttca aaggtgtgca cccggaagtc ggacttaccc attgggaaac
     6601 gccccggata agtctgtact tttttaccgt tttcaatatc gaaaccttcg gtaaatttca
     6661 gcgacttaat ttcatccagc gtgaaatcta tcgcgtagta acgaccgtct ttgcgcgccc
     6721 gatccgggaa acgatcggca acatcagtaa cacgatcgag ataatggtca tgcagaacaa
     6781 ccagatggtc gtctttggtc atcaccaaat cctgttccag ataatccgct ccctgcgcat
     6841 acgccatcgc ttttgctggc agcgtatgct ccggcaaata tccactggca ccgcgatggg
     6901 cgatgactat tttttcgttg ctgtccgccg ccattgcact gcttcccatg actatagtgc
     6961 tcatcatgat cgccatgcta aggtttttca gcttcaattt catgccatta gcctccgttg
     7021 cgtttttgca gtaattgttc atggcgacgt ttttcgccaa tcatcacaac aatcaacaag
     7081 ataaccgcca gaatgctgcc gccaatcatt accataaagc cgccatccca gccgaagaag
     7141 tccacggtgt agccaacaat cgcgctcgcc gccaccgaac cgcccaggta accaaacagc
     7201 ccggtaaagc ccgctgccgt acctgccgct tttttcggtg ccagttccag cgcatgcaga
     7261 ccgatcagca tcacaggacc gtagatcagg aagccgataa caatcataca aatcatatcg
     7321 acggttgggt tacctgccgg gttcatccag taaacgatag tcgcgatggt caccagtgtc
     7381 ataaagaaga cgccggttgc cccacggtta ccacggaaga ctttgtccga catccagccg
     7441 cacagcaggg tgcccggaat acccgcatac tcatagaaga agtaggccca ggaggattta
     7501 tccagcgcga aatgcttaac ctctttcaga taagtcggtg accagtcgag gatgccgtaa
     7561 cgcagcagat aaacgaacac gttggcgatg gcgatgtacc acagcagttt gttcggcagc
     7621 acgtactgca tgaagatttg cttcgcggtc agctcctgtt ccgctttttc gttatagtcg
     7681 tccggataat catttttgta ctcttcgatc ggcggcaagc cacaggattg cggggtatcg
     7741 cgcatcatcg caaaggcgaa taatgccacc agaatggcgc agaaagcagg catatagagc
     7801 gccgcatgcc agtcattgaa ccaggccatc cccagcagga acagcagcgg cggaatacca
     7861 ccaccgacgt tgtgcgcaca gttccacact gacacaatgc cgccacgttc tttctgcgac
     7921 caccagtgca ccatagtacg accacacggc ggccacccca tcccctggaa ccaaccgcag
     7981 aggaacaaca gtacaaacat caccgcaatg ctcgacgtcg cccatggcac aaagcccata
     8041 aacaacatca ctgccgccgc cagaatcaaa cctgcgggca ggaaaacgcg cggattcgag
     8101 cgatccgata ccgaacccat gatgaatttc gaaaatccat aagcaatcga gatccccgaa
     8161 agggcaaaac ctaaatcacc gcgtgagaat ccctgctcaa ccagataagg catagcaagc
     8221 gcaaagttct tacgaaccaa atagtaagcc gcatagccaa agaatatccc caggaaaatt
     8281 tgccagcgca atcgacgata agtcggatcg atctccgcgg caggtaagcg cgctttgtgt
     8341 ggcgctggtt taaaaatact caacattgat agcctccgtg gcccgtggtc ttatttatga
     8401 ttaacagcct gattcagtga gagaacctgc cgtttcttga gttgccgcga tgttaagaaa
     8461 acattcataa attaaatgtg aattgccgca cacattatta aataagattt acaaaatgtt
     8521 caaaatgacg catgaaatca cgttttactt tcgaatcatg agcgaatatg cgcgaaatca
     8581 aacaattcat gtttttacta tggctaaatg gtaaaaaacg aacttcagag ggataacaat
     8641 gaaaactcgc gactcgcaat caagtgacgt gattatcatt ggcggcggcg caacgggagc
     8701 cgggattgcc cgcgactgtg ccctgcgcgg gctgcgcgtg attttggttg agcgccacga
     8761 catcgcaacc ggtgccaccg ggcgtaacca cggcctgctg cacagcggtg cgcgctatgc
     8821 agtaaccgat gcggaatcgg cccgcgaatg cattagtgaa aaccagatcc tgaaacgcat
     8881 tgcacgtcac tgcgttgaac caaccaacgg cctgtttatc accctgccgg aagatgacct
     8941 ctcattccag gccactttta ttcgcgcctg cgaagaagca gggatcagcg cagaagctat
     9001 agacccgcag caagcgcgca ttatcgaacc tgccgttaac ccggcactga ttggcgcggt
     9061 gaaagttccg gatggcaccg ttgatccatt tcgtctgacc gcagcaaaca tgctggatgc
     9121 caaagaacac ggtgccgtta tccttaccgc tcatgaagtc acggggctga ttcgtgaagg
     9181 cgcgacggtg tgcggtgttc gtgtacgtaa ccatctcacc ggcgaaactc aggcccttca
     9241 tgcacctgtc gtggttaatg ccgctgggat ctgggggcaa cacattgccg aatatgccga
     9301 tctgcgcatt cgcatgttcc cggcgaaagg atcgctgctg atcatggatc accgcattaa
     9361 ccagcatgtg atcaaccgct gccgtaaacc ttccgacgcc gatattctgg tgcctggcga
     9421 taccatttcg ctgattggta ccacctcttt acgtattgat tacaacgaga ttgacgataa
     9481 tcgagtgacg gcagaagagg ttgatattct gctgcgtgaa ggggaaaaac tggcccccgt
     9541 gatggcgaaa acgcgcattt tgcgggccta ttctggcgtg cgcccgctgg ttgccagcga
     9601 tgacgacccg agcggacgta acgtcagccg tggcatcgtg ctgctcgacc atgctgaacg
     9661 cgatggtctg gacggattta tcaccatcac cggtggcaaa ctgatgacct atcggctgat
     9721 ggctgaatgg gctaccgacg cggtatgccg caaactgggc aacacgcgcc cctgtacgac
     9781 tgccgatctg gcactgcctg gttcacaaga acccgctgaa gttaccttgc gtaaagtcat
     9841 ctccctgcct gccccgctgc gcggttctgc ggtttatcgt catggcgatc gcacgcctgc
     9901 ctggctgagc gaaggccgtc tacaccgtag cctggtatgt gagtgcgaag cggtaactgc
     9961 gggtgaagtg cagtacgcgg tagaaaattt aaacgttaat agcctgctgg atttacgccg
    10021 tcgtacccgt gtggggatgg gcacctgcca gggcgaactc tgcgcctgcc gcgctgccgg
    10081 actgctgcaa cggtttaacg tcacgacgtc cgcgcaatct atcgagcaac tttccacctt
    10141 ccttaacgaa cgctggaaag gcgtgcaacc catcgcctgg ggagacgcac tgcgcgaaag
    10201 cgaatttacc cgctgggttt atcagggact gtgtggtctg gagaaggagc agaaagatgc
    10261 gctttgatac tgtcattatg ggcggcggcc tcgccggatt actctgtggc ctgcaactgc
    10321 aaaaacacgg cctgcgctgt gccattgtca cgcgtggtca aagcgcgttg catttctcat
    10381 ccggttcgct ggatttgctg agccatctgc cagatggtca acccgtggca gacattcaca
    10441 gtggactgga atctttgcgt cagcaggcac ctgcacatcc ttactctctt ctcgggccac
    10501 aacgcgtgct cgatctcgct tgccaggcgc aggcattaat cgctgaaagc ggtgcgcaat
    10561 tgcagggcag cgtagaactg gctcaccagc ggattacgcc gctcggcact ttgcgtgcaa
    10621 cctggttaag ttcgccagaa gttcccgtct ggccgctacc cgcgaagaag atatgtgtag
    10681 tggggataag cgggctgatg gatttccagg cgcatcttgc ggcggcttcg ttgcgcgaac
    10741 tggatctttc ggttgaaacc gcagaaatag agctgccgga actggatgtc ctgcgcaata
    10801 acgccaccga atttcgcgcg gtgaatatcg cccgtttcct tgataatgaa gaaaactggc
    10861 cgctgttact tgatgcgctt attcctgtcg ccaatacctg cgaaatgatc ctgatgcccg
    10921 cctgcttcgg cctggccgat gacaaactgt ggcgttggtt gaatgaaaaa ctaccttgtt
    10981 cactgatgct tttgccaacg ctgccgcctt ccgtgctggg cattcgtctg caaaaccagt
    11041 tacagcgcca gtttgtgcgc caaggtggag tgtggatgcc gggcgatgaa gtgaaaaaag
    11101 tgacctgtaa aaatggcgta gtgaatgaaa tctggacccg caatcacgcc gatattccgc
    11161 tacgtccacg tttcgcggtt ctcgccagcg gcagtttctt tagtggcgga ctagtagcgg
    11221 aacgtaacgg cattcgcgag ccgattctcg gccttgatgt gctacaaacc gccacgcggg
    11281 gtgaatggta taagggcgat ttttttgcgc cgcaaccgtg gcagcagttc ggtgtaacca
    11341 ctgatgagac gctacgcccg tcgcaggcag ggcaaaccat tgagaacctg tttgccatcg
    11401 gttcggtgct gggcggattt gatcccatcg cccagggatg cggcggcggt gtttgtgccg
    11461 tcagtgcttt acatgccgct caacagattg cccaacgcgc aggaggccaa caatgaacga
    11521 caccagcttc gaaaactgca ttaagtgcac cgtctgcacc accgcctgcc cggtgagccg
    11581 ggtgaatccc ggttatccag ggccaaaaca agccgggccg gatggcgagc gtctgcgttt
    11641 gaaagatggc gcactgtatg acgaggcgct gaaatattgc atcaactgca aacgttgtga
    11701 agtcgcctgc ccgtccgatg tgaagattgg cgatattatc cagcgcgcgc gggcgaaata
    11761 tgacaccacg cgcccgtcgc tgcgtaattt tgtgttgagt cataccgacc tgatgggtag
    11821 cgtttccacg ccgttcgcac caatcgtcaa caccgctacc tcgctgaaac cggtgcggca
    11881 gctgcttgat gcggcgttaa aaatcgatca tcgtcgcacg ctaccgaaat actccttcgg
    11941 cacgttccgt cgctggtatc gcagcatagc ggctcagcaa gcacaatata aagaccaggt
    12001 cgctttcttt cacggctgct tcgttaacta caaccatccg cagttaggta aagatttaat
    12061 taaagtgctc aacgcaatgg gtaccggtgt acaactgctc agcaaagaaa aatgctgcgg
    12121 cgtaccgcta atcgccaacg gctttaccga taaagcacgc aaacaggcaa ttacgaatgt
    12181 agagtcgatc cgcgaagctg tgggagtaaa aggcattccg gtgattgcca cctcctcaac
    12241 ctgtacattt gccctgcgcg acgaataccc ggaagtgctg aatgtcgaca acaaaggctt
    12301 gcgcgatcat atcgaactgg caacccgctg gctgtggcgc aagctggacg aaggcaaaac
    12361 gttaccgctg aaaccgctgc cgctgaaagt ggtttatcac actccgtgcc atatggaaaa
    12421 aatgggctgg acgctctaca ccctggagct gttgcgtaac atcccggggc ttgagttaac
    12481 ggtgctggat tcccagtgct gcggtattgc gggtacttac ggtttcaaaa aagagaacta
    12541 ccccacctca caagccatcg gcgcaccact gttccgccag atagaagaaa gcggcgcaga
    12601 tctggtggtc accgactgcg aaacctgtaa atggcagatt gagatgtcca caagtcttcg
    12661 ctgcgaacat ccgattacgc tactggccca ggcgctggct taaactccct tctgatgccc
    12721 ggtaagcatg tggttaccgg gcatttttgc gtacacgatt ccgtgcccaa tgtatgcgtt
    12781 gcaacgcagt gaaaattcct ctgaaaacgt ctcgcaaagg ctgaaactgg cagatgtcaa
    12841 aggcctggga taaccgtaat gtcgcgtcat cataaatatc aggtgacgga caaccatgac
    12901 cgaatcaaca acctcctccc cgcatgatgc ggtatttaaa acctttatat tcacacccga
    12961 aaccgcacgg gattttctcg aaatacattt accagaacca ctgcgcaagc tttgcaacct
    13021 gcaaacctta cgcctggaac ccactagttt tattgaaaaa agtttacgcg cttactactc
    13081 ggatgttttg tggtccgtgg aaaccagcga cggtgacggc tatatctact gcgtgattga
    13141 acatcaaagc tctgcagaaa agaatatggc ttttcggcca atgcgctatg ccactgccgc
    13201 catgcagagt cacctagata aaggctatga cagagttccg ctggtggtgc cattgctgtt
    13261 ttatcatggc gaaacatcgc cctacccgta ctcacttaac tggctggatg agtttgacga
    13321 tccgcaactt gcccggcagt tgtacaccga agcttttccg ttggtggata tcaccatcgt
    13381 acctgacgat gagatcatgc aacatcggcg tatagctctg ctggaactga ttcaaaagca
    13441 tattcgcgac cacgatttaa tcggcatggt cgacaggatc accacgcttt tggttagagg
    13501 cttcactaat gacagccagc tacaaacact gtttaattat ctgctgcaat gcggcgatac
    13561 ctcccgtttc acccgtttta ttcaggagat tgccgaacgt tcaccactac aaaaggagag
    13621 attaatgact attgctgaac ggctacggca ggaaggacat caaattggct ggcaagaagg
    13681 taaattagta ggtttgcagc aaggtaaatt agaaggtttg caggaaggta tgcatgaaca
    13741 agccattaaa attgctttgc gcatgctgga acagggcatt gatcgtgacc aagtgctcgc
    13801 ggccacccag ctaagcgaag ccgatctggc agcgaataac cactaattaa cacaggccca
    13861 cagccgatcc ccatgggcct ttgatatcaa taactacctt ttatgcgtgg cccatttttg
    13921 cctgatttaa acatcgccag tcgttgatcc agggcatcgc tgtagagcat cgtgtcaacg
    13981 ccaacagcga caaagttcgc tccccacgcc aggcattgct gcgccatatc aggagccaca
    14041 gccagaaaac cagccgcttt acccgcagca cggatccgcc gaatgctggt ttcaataatt
    14101 cgctgcactt ccgggtgccc ggcgttatcc gggtagccca acgacgcaga aagatccgct
    14161 ggtccaataa acacgccatc aatcccttcg acgtcgagga tctcgtccag gttatccagt
    14221 gccgttttac tttccacctg cactaacaga caaagcgaat cgttgacttg cgccatgtaa
    14281 ttctcaatgc gtccccagcg cgcagcccgt gccacactgg ccccaacacc acgctcaccg
    14341 tagggagggt tggacaaatt ttgccggagg gggaatgtgc cggtgcagta aaccggcaca
    14401 gagtggcgaa atcagacgga taaagtcatg gactcaacaa cctcaatcca gccatgctca
    14461 ctagcgatgt cctggccgtt taaccaacgg cgtagcatat tcagtgccat cattgcgcac
    14521 acttcctgac ggatagccag gctgtagcgt gtagtgctga aacgcacacg cagagcgaaa
    14581 gtgccgtctg gcgtggctag cgcaaagttg agatgctcgt tctcgaaacc cgaaacagcc
    14641 agtgccagcc cggcaaaatg gttggcccgc cgttctgtaa tccagtgcgc agtttgcgcc
    14701 agggtttctt cctgtgaagg aatcacttca cacgccagca atggagcacc tgcgcgagaa
    14761 agttgcaaag ccaataaacc accggtgaat tgctcgctca acgtcaggct gaactggcgg
    14821 ttttgcaatt cgcgactgat ctgcgcgggc agtccttcgg tgccttcgaa aatcacgctc
    14881 tgtccggcaa cacgtttaac gtccagccac agtttttcca tcgcctgttg ctcgcttgcc
    14941 ggtccggtga gtttcagttc gatgatcggc attgaggagc gatagcccat tgttacgccc
    15001 ggcggcagtt gtagagtgtc caggctttgt gccagatcgc tttccgaacg accaaaggta
    15061 gttaaacgca gacaaaccgg cggctgtggt aaagaaaaac gctcgcgcag gcgcggcagg
    15121 atttcgtgct cgaccatcac cttaaattct gacggtacgc cgggagtaaa gaacatcagg
    15181 caacgattaa gctgcacggc aaaaccacag gcggtgccta ccgggttatt gataaattca
    15241 gcactggcag gcagctccgc ttgtttacgg ttgctcggtg ccattactcg tccacgttcg
    15301 tggaaatagc gttccatctc tttgagccag gcttcatgca gcaccaggcc ttcacctttt
    15361 gctgtcgcag cggcgagtgc gcttaaatca tcgctggtcg gtcccagccc gccgttaacg
    15421 atcagcacat cggcgtgctg actacgttcg cgaagaatgg tgactaagtc atcaaggtta
    15481 tcccccaccg tattgcggcg agataatggc agcccctgat gaaagaaaaa atcggccagc
    15541 caggcagcgt tagtgtcaac gatttgcccg tataacactt catccccggt ggatagcatt
    15601 tccactttta acatggttat ctcctgcttt tgttatcgaa tcactataac gcaaagggag
    15661 ataaggcgta gtaagaaact gaatgccgcg ccggaaggga tcagaaactg gcgttaaccc
    15721 caacatacag gccgtcagcc acagcgttgt cgcggttacc gtctttaccc gacagattca
    15781 ggtagcgata acccgcctca atactgactg gacgcataat ggtgtaacgc gcgccagcat
    15841 tcgcttcctc ataactttga ataccgctgg agagcgaatc cggagagtag taatactcgc
    15901 caaacaaacg gaagctgttg ccaattttcc actgcaaacc acctcctacc gctgcggcat
    15961 aaccttcatc gccgtaattc gggttggtgt acacgccttt tccgccaacg gtcgccatta
    16021 acggcccgag aggcagattc aaccccagcc ccacgcccgc gacgtcaccg tcgtcgtcgt
    16081 tatgtgtcca gttaccgctt aaagccaggc ccgtcgattc agtcccaaaa ccgacaccaa
    16141 tattggtgta ttctttaccc gcctggccgc tgatgctcat tgcattgacc gatgcagaaa
    16201 ccaacagcat tccggccaga cctgctagcg caattttttt cattatcccc tttcccacaa
    16261 gcaattaatc aaaattccca aagctgcggg attgtactgc ctggcacaga ggattcaatg
    16321 agcgaaaaaa gggatctagt cacttattgt aactaaatac ttcttaaata attaaacgcc
    16381 gaaaaaataa acacacttta ccgatacaca caaaaataat aataagtgca gttatcattg
    16441 caaaagagat tactgtcact ttcacatatt tctgagttac cgtattctca tggtttcttt
    16501 ttatttataa ggaatacccc tgtgcgacaa cggactattg tatgcccttt gattcaaaat
    16561 gatggtgctt atttgctgtg caaaatggcc gacgatcgcg gcgttttccc cggtcaatgg
    16621 gcgctttcgg gtggcggcgt ggagtctggc gaacgaattg aagaggcact acgccgcgaa
    16681 attcgcgaag aactgggaga acagctgctt ttgacagaaa tcacgccgtg gaccttcagc
    16741 gatgatattc gcaccaagac atatgcagat ggtcgcaagg aagagattta tatgatttac
    16801 ctgatttttg actgcgtttc tgccaaccga gaagtgaaaa taaacgaaga gtttcaggac
    16861 tacgcgtggg taaaacctga agatctggtg cattatgatt taaatgttgc cacccgaaaa
    16921 acgttacgtt tgaaaggtct tctttaacaa tgatgacagc catcactggc tgtcagattg
    16981 aaaatttagt ggttaacgaa ttccccatcc agataaactt tgcctttttc cacatgcatg
    17041 actaaaccat ccagataatc aggtttaaat atcgcatcac gcttattttt agcaatatat
    17101 gtcaggcaat gattatgggt gaaaataacg atatttttat caggcgcttt gttttgtaag
    17161 tccttaattg cactataaat ctcattaccg cactgaagaa gtcgtttatc taccgtcagc
    17221 tttttacccg cggaaaacca ggtcgccgac tgaatggtcc ggacggtatt actggaataa
    17281 agatcgaaat cagagatgtc agcgctaaaa gcgttgccca gttcacgggc atcctgggta
    17341 cctttaaccg taatacctgt tttatctgac aagcattgat tggttgaacg atcgcaacgt
    17401 tcagcatggc gaaacaaaac gacaaccggg tgctgctgtg ccagtctggc caatgtttta
    17461 ttgtcgatac gtggcaaacc attgctactc caggcggcat gagtacccag tccggcaatt
    17521 gcagcgagcg ccagtaaaat gatgaaatat ttttttgact tcaatgatga gcggcaaaaa
    17581 gctaacacgt aaactccacc tatagacaag cgcaaccaga caattaccgt gaaattgagc
    17641 gacatttctg gcgataatta gcagttggtg taatattaaa aatcctacga tgtcggcaaa
    17701 atgcctcaaa tttttgccaa atgcaaagcc taaataagaa aaaatataaa aatttcaata
    17761 tttacgtcta atattagttt cttaaggtta agttaatatt ctatccttaa aatttcgctc
    17821 caaatggcaa aatatacaca acactcttta tagcaaatat aagtggacag gtattcaatg
    17881 gcggaaggaa aagcaatgtc agaatttttg cctttttcgc gaccagcaat gggcgtggag
    17941 gaactcgctg cagttaaaga ggttctcgaa tccggttgga tcacaaccgg tccgaagaat
    18001 caggcgcttg agcaagcctt ttgccagttg acgggaaatc agcatgccat cgcggtcagt
    18061 tcagccaccg ccggaatgca tatcacgcta atggcgttgg aaattggcaa gggcgatgaa
    18121 gtgattacgc cttccctgac ctgggtttca accctcaata tgatttcctt gctgggtgca
    18181 acgccggtaa tggtggatgt cgaccgcgat acgctgatgg tcacgcctga agcgatcgaa
    18241 tcagccatta cgccacgcac taaagccatc attccggtgc attatgccgg tgcgccagca
    18301 gatattgacg ccattcgcgc cattggcgaa cgttacggca tcgcagttat cgaagatgct
    18361 gcccatgccg tcggtacgta ttacaaaggg cgacatattg gcgcaaaagg taccgctatt
    18421 ttttcatttc atgccattaa aaatattacc tgtgctgaag gtggcctgat tgtaactgat
    18481 aatgaaaacc ttgcccgcca gctacggatg ctgaaatttc acggtctggg tgtcgatgcc
    18541 tatgacagac aaacctgggg ccgtgcaccg caggctgaag tcttaacacc gggctataag
    18601 tacaatctga ccgatattaa cgccgcgatt gccctgacac agttagtcaa attagagcac
    18661 ctcaacaccc gtcggcgcga aattgcccag caatatcagc aagcactggc agctctcccc
    18721 tttcagccat taagccttcc cgcctggccg cacgttcacg cctggcatct gtttattatt
    18781 cgtgtcgatg aacaacgttg tggtatcagt cgcgatgcgt tgatggaagc gttaaaagaa
    18841 agaggcattg gtaccgggtt acatttccgc gccgctcaca cacaaaaata ttatcgcgag
    18901 cgttttccca cgctgtcgtt accgaatacc gaatggaata gcgaacgcat ctgttcgttg
    18961 ccgctgttcc cggatatgac taccgccgat gccgaccgcg tcatcacagc ccttcagcaa
    19021 ctcgcaggac aataagccat gtttgaaatc caccctgtta agaaagtctc ggtggttatt
    19081 cccgtttata acgagcagga aagcttaccg gaattaatca ggcgcaccac cacagcctgt
    19141 gaatcgttgg ggaaagagta tgagatcctg ctgattgatg acggcagtag cgataattcc
    19201 gcgcatatgc tggtcgaagc ctcacaagcg gagaacagcc atattgtgtc tattttgatt
    19261 aaccgcaatt acgggcaaca ttcagcgatt atggcgggat tcagtcacgt tactggcgac
    19321 ttaattatta cccttgatgc cgatctccag aatccgccag aagaaatccc ccgcctggtg
    19381 gcaaaagccg atgaaggtta cgacgtggta gggactgtac gccagaaccg ccaggacagc
    19441 tggtttcgta aaacagcttc gaagatgatt aaccggctta ttcagcgcac cactggcaaa
    19501 gcgatgggtg actacggttg tatgctgcgc gcctatcgcc gtcatattgt cgatgcgatg
    19561 ttgcactgcc atgaacgcag cacctttatc ccgattctgg cgaatatctt cgcccgccgt
    19621 gcaattgaaa ttccagtaca tcatgccgag cgtgagtttg gtgaatccaa atacagtttt
    19681 atgcgcctga ttaatttgat gtacgacctg gtgacctgcc ttaccacaac gccgctacgt
    19741 atgctgagtc tgctcggcag cattattgcg attggaggtt ttagcattgc ggtgctgctg
    19801 gtgattttac gcctgacctt cggaccacaa tgggcggcag aaggcgtctt tatgctattt
    19861 gccgtgctgt ttacttttat tggcgctcag tttatcggca tgggattact cggtgaatat
    19921 atcggcagga tctacaccga tgtccgcgcc cgcccccgct attttgttca gcaagttatc
    19981 cgtccatcca gcaaggaaaa tgaataatga aaaccgtcgt ttttgcctac cacgatatgg
    20041 gatgcctcgg tattgaagct ctgctggctg ccggttacga aattagcgcc atttttaccc
    20101 ataccgataa tcccggtgaa aaagcctttt atggttcggt ggctcatctg gcggcggaaa
    20161 gagacattcc ggtttatgcg ccggataacg ttaatcatcc gctgtgggtg gaacgcattg
    20221 cccaactgtc accagaggtg attttctctt tttattatcg ccatcttatt tgcgacgaga
    20281 tttttcagct cgctcccgca ggtgcattta atctgcacgg ttcgctgtta ccaaaatatc
    20341 gtggtcgcgc gccgctgaac tgggtgctgg tgaacggtga aacggaaacg ggcgttacat
    20401 tgcaccgaat ggtaaacgtg ccgatgctgg ggccattgtg gcgcaactgc gcgttgccat
    20461 tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg cgcgccagct
    20521 actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg cccagcgcga
    20581 aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttccttg aatggcataa
    20641 accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc cgggtgcctt
    20701 cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag
    20761 caaagcacag ccggggagcg tgatttctgt tgcgccactg ctgattgcct gtggcgatgg
    20821 cgcgctggaa atcgtcaccg gacaggcggg cgacggcatt actatgcagg gctcgcaatt
    20881 agcgcagatg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg cctgcaccgc
    20941 ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca accatctgac
    21001 agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg gcagcgatgc
    21061 gataagccgt tttctgaatc atccacattt tcactttgtc gaaggcgata tcagtattca
    21121 ttccgaatgg attgagtatc acgtcaaaaa atgtgatgtc gtcttgccgc tggtggcgat
    21181 agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg attttgaaga
    21241 gaatctgcgc attatccgct actgcgttaa gtaccgtaag cgaatcatct tcccgtcaac
    21301 ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt ctaatttaat
    21361 cgtcggcccg gtgaataaac cacgctggat ttattcggtg tcaaaacaat tacttgatcg
    21421 ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc tcccgtttaa
    21481 ctggatggga ccacgactgg ataaccttaa tgcagcgcga attggcagct cccgcgctat
    21541 tacgcaactc attctcaatc tggtagaagg ttcaccaatt aagctaattg atggcggaaa
    21601 acaaaaacgc tgctttactg atatccggga cggtatcgag gcgttgtacc acattatcga
    21661 aaatgcggga aatcgctgcg acggcgaaat tatcaacatt ggcaatcctg agaacgaagc
    21721 gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca
    21781 tcatttccca ccgtttgcgg gctttcgcgt tgtcgaaagt agctgctact acggcaaagg
    21841 atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccaccgct gcctggactg
    21901 ggagccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct tcctgcgcac
    21961 cgttgatctt acggataaac catcatgacc aaagtaggct tacgcattga tgtcgatgcc
    22021 tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt
    22081 caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct ctggcgactg
    22141 gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct ttatggctgg
    22201 gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc caatgccgat
    22261 atcattcgtg aagcggctaa acatcacgaa gtcggtctgc acgcctggga tcaccatgcc
    22321 tggcaagccc gtagcggtaa ctgggatcgg caaacaatga tcgacgatat tgcacgcggt
    22381 cttcgcactc tggaagagat tatcggtcaa ccggtaacct gttctgccgc tgcgggctgg
    22441 cgtgccgacc agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat
    22501 tgtcgtgggg ccataccgtt ccgtccactg cttgaatcag gaaaccctgg cactgcgcaa
    22561 attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa agcagaagat
    22621 tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt ttataccatt
    22681 catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct caaacgcgca
    22741 gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagagac gttgccgctc
    22801 ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg ccaacaaatt
    22861 gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg catcttcgcg tttattgcct
    22921 gctattacct gttaccgatc agcacgcgtc tgctctggca acccgatgaa acgcgttatg
    22981 cggaaatcag tcgagaaatg ctggcatccg gcgactggat tgtgccccat ctgttagggc
    23041 tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggg caatggctat
    23101 ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg
    23161 cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct ctactcgcca
    23221 cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc gtgctcgatc
    23281 cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctggca atgcaggcac
    23341 agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc ggcatggggg
    23401 tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg ccatgggtag
    23461 cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt atcagttgcg
    23521 tactgacggt tctcccctgg ggactggcga tagcgcagcg ggagcctgac ttctggcact
    23581 attttttctg ggttgagcat attcaacgct ttgcactaga tgatgcccaa catagagctc
    23641 cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg ggattactcc
    23701 ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga
    23761 gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggcaaattg cccacctata
    23821 ttctttcttg ctttgcacct ctggcaatgc tgttggcgca ttacgcgttg ctggcagcaa
    23881 aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt ggcgtcactg
    23941 gcattattgc cacatttgtg atctccccct ggggaccaat gaacacgccg gtgtggcaaa
    24001 ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg
    24061 gctggtacac cttaacaaac gtcgaaaaga cctggtcttt cgccgcgctt tgcccgctgg
    24121 ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga aaacatccgc
    24181 aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt cttactgata
    24241 gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc atcatgtatc
    24301 gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg agatttgtca
    24361 gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attacactcg
    24421 tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc gcagatgcca
    24481 tcgttcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct ggctaacatt
    24541 agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt
    24601 tgtggcgata aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg
    24661 tcttggtctt gccatgatgc tctggctgct ggtcttgcag aacgtaccgg taggcattgc
    24721 ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa aactgtggca
    24781 cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggtg gcattgtgat
    24841 cctcgggagt acggtgtaat gtgcctgata tggggattat tcagcgtcat tattgcctca
    24901 gttgcgcaat taagcctggg ttttgcggca agtcatctgc cgccgatgac gcacttatgg
    24961 gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt actcgggctg
    25021 ctgggttatc tgctttccgt tttttgttgg tataaaacgc tacataaact tgcgctaagc
    25081 aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt
    25141 cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc
    25201 gggttgatgc tgattttcct gcccacgaca aaacaacgtt actgagtttt ccctgccgct
    25261 gtacactgcc gttcccactc atcggggcta taactgcttg ccgagaggac tttcaccgta
    25321 tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag taaatccccc
    25381 actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg
    25441 cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt gtgccagcca
    25501 ttccattgca ttatcctgtt tgctaagagt tttcacatca atttacagat taacagataa
    25561 actctttatc aacggcaatg gaatggcaga aatgagaagt gaaacggagg gttaattatt
    25621 gctgacgttg cacccactct tttagcgcct gacgtgaaat tttaataccg ccgtttttca
    25681 gctccggcgg taaagtcagc cagcgcaccg gttgttgaaa acgagccagc ttatctttca
    25741 cccattcgcc aagatcaacg gtttgctggt catactccac caccgctacc ggccgatgac
    25801 caaactcctt gtcggcaacg gaaacgataa acacctgcaa aaccgcagga tgtgcagcaa
    25861 taacacgctc gacttcttcc ggctgaatac cctcaccgcc actgaagaac agattgtcta
    25921 aacgtccgac aatggtcagc ttgccattat gcatctcacc gcgatcgcgc gtagcgtacc
    25981 agccttcgtc attaaccagt gaaaccagtt gcccgttacg ccagtaacct tctgccatac
    26041 tggcagcccg cagccacact tcattattaa cgattttcac ttcccgaccc ggcagcggca
    26101 aaccaacgtc tgccaggccg tcggcttctt tcgcacatac cgtggaggca aactcggtca
    26161 gaccatagcc gcaaaagcaa cgaatcccct gctcgcgcgc ctgttccgtc aactcgaccg
    26221 ggatagccgc gccgccaagt aacaccgctt tcagggaaac ggaactgcgg ttaaccagca
    26281 aacgccagag ttgtgttggc accagtgaag cgtgagtaca gcctgccagc atttgctcca
    26341 atggctgttt atcacgcacc gtcatccgcg caccagcgta taaccagcgc cacataattc
    26401 cctgaccgga gacgtgaaac agcggtaaag agagcaacca gtcatcgtga tcgccaaacg
    26461 gaatcagcga taacacacct tgcgcactgg caagatgggc ctgataagta tggacagcgg
    26521 ctttcggcaa accggtagaa cccgaggtca acgtcattga gcacagacgc gtcggctgcc
    26581 acgcagcggc atgtacgcct tcaaccagct gaatgtgcag cgacgttaat gccggaaacg
    26641 tgttttcccc atccggcacc agcgcaaatt gcagcgtcag attgggcagc aattcttcaa
    26701 gcaacggttg cggcagctga gggttcacgg gcaacacccg cgccccgcat tgcagtaacg
    26761 ccagccaggc gagcagcgtt tgcggcgtat tccacgcccg caacatcacg ccgctgccct
    26821 caaccacccc ctgcgccgca aagcccgtag ctaactcatc gacgcgagca caaagctcgc
    26881 gccagttgag ttgctcgtca ttaagacgta aggcgatggc ttctccccgc acttgccgcc
    26941 agtgacgcca cggccagtca gagaagatca taacaactgc tcctgtacat cagcgtccac
    27001 taacggcagt gggctccccg gccagcgacg aacctgctgc gcctgcatca gatccagcgt
    27061 gtccagtccc ggaatggtgt ctggcgttaa ccaggcagca atccgcgcca gttgcgttag
    27121 acctaagctc gactcaatgg aagaactgat caccgccgtc agccccagcg catgcgccgc
    27181 ctgtacctgc tcgcgtactt tttccagact gcccgtgagc gtgggtttga taactaccgc
    27241 gcgcacgccc tcttcagcca caaaggcaaa atccggctcg cgcaggcttt catcccaggc
    27301 aatggcaatg ccggtttcac gggcaaacgc tcgcgaatca tcgcgggttt tgcacggctc
    27361 ttcgagaaac gcgatgcggt ggcgataatc cgggttaaca tatttggcaa actgctgacc
    27421 tttcagcggt gtccaggcgc gatttgcgtc aagacgcaaa tgcagatccg gaattgcctc
    27481 cagcaacaga ttcaccacca tgccgtcgcg caccgcttcg tacaatccca ctttgacctt
    27541 cgccactttc tcgcccggca tatcggcaag tttgaggatc agatcgtccg gatcgccatt
    27601 acacagcggt gccgcacggt agttggctgc ttgcggcaac gtatctgtca gttctgccaa
    27661 tgcacagctt acgccaaagg ccacggaagg catctgcggt agctcgcaat cgcctgccag
    27721 ccagttattt acccaggcaa gcagcacgct ttgcgcctct tcccagtttt cctggctgaa
    27781 gcccggcagt ggggagatct ccccccaccc ttcgcgctcg ccctcacgca ggcaaacgta
    27841 cagcccgtcg cgggttttta accgcctgtc gcgcagaacc acccccgcgt ccatggggat
    27901 ctgccagcgg tatacctgcg cgctacgcat tacggattcc gtttaaattt gctgaaatca
    27961 ggctgacgtt tctggttgaa agcgttgcga ccttcctgac cttcttccgt catgtagaac
    28021 agcatggtgg cgttgcccgc cagctcctgc agccccgcct gcccgtcaca gtcggcgttc
    28081 agcgcagctt tcaggcagcg cagcgccatc gggctgtttt gcagcatttc gcggcaccaa
    28141 cggacggttt ctttttccag atccgccagc ggtaccacgg tgttgacaag gcccatatcc
    28201 agcgcctgtt ttgcgtcgta ctgacggcac aggaaccaga tttctcgcgc ttttttctgc
    28261 ccgacgatac gagccatgta ggaagcgccc cagccgccgt cgaaggaacc gactttcggg
    28321 ccagtctgac cgaagatggc attatctgct gcgatagtca ggtcgcacat catgtgcaga
    28381 acgtgaccac cgccgatgga gtagccagcc accatcgcga caaccggttt cggacaggta
    28441 cggatctgac gctggaagtc cagcacgttc aggtgatgta cgccggaatc atctttatag
    28501 ccgccgtaat caccacgcac tttctggtca ccaccggagc agaacgcttt atcgcctgcg
    28561 ccagtcagaa tgatcacgcc gatattgtcg tcataacgcg catctgccag tgcctggatc
    28621 atctctttta ccgtcagagg acggaaggca ttgcgcacct gcggacgatt aatggtgatt
    28681 tttgcgatac cgtcggtgga tttttcataa cgaatgtcct cgaaaccttc ggagcagtcg
    28741 tgccattcaa ccggtgcgta aggcattgct tcatcaggat aaatcatatt gtgtccttta
    28801 gtcagaaacg caagatctgc gccagacttg cgattacgcc agcgggattt tcccgatgcg
    28861 cgttatgtcc ggcgcgagga atgacatggc agtcggcagc cagttccgcc gccagggcgc
    28921 ggaatttgct gtcacgttca ccacataaat aataaaacgc aaatgtgcgg gcgctaaggt
    28981 tagcacgtaa atcaggctgg acggcgagag aagtcgcctc cagcatggcg gcaagggttg
    29041 cgccattatt gttgctgcgc agcgccacca gctcccggcg ttgatcgtca ttgagcgagg
    29101 caaaaacagg ctgttgatac cagtcggcaa ataccgccgt taacggttct gtgcgaaaac
    29161 gctgcgccca ttggcgatcg gaacgctgac gttccgcacg ttgttcagca ttttgcagcc
    29221 ccggatgccc gccttcgaca ataaccccac aaagccccgc cagcccctgg caagccgcca
    29281 tcatcgccac ccgtccgcca agcgagtacc ccaccagcca gaagtcaagg atgttgtaac
    29341 taaccaaggt tttacgcagt aagtcggtga catcatcaaa tccatcgacg ctaatcgccg
    29401 ccgaaccacc atgacctagg agatcaacat acaaccgtga gtagtcggca aacgcctcgc
    29461 ccacttcttg ccattcgtgg caatcgccgg aaaaaccgtg caaaaacacc agccagggta
    29521 aacctagttt tccgtgtttt gcctgcgcgt gcaggatcat aaatggctta cctgcgccag
    29581 aagttgctgg agcgtttgcg caccatcggt gtcgttaacc accatttcaa tcaccgtggt
    29641 ggttggcgtg cgccaggcgt cggcaaatgc cgtttcaagt tcctgccagt tttgcggatg
    29701 atgatatttc agctcgaaca tcgcggcggc gtgctcaaaa tggacgtttt gcggcatcag
    29761 atagaaacgc tcacgctcgc tttgcggcgt tggcaacagc gagaaaattt gcccgccgtt
    29821 gttgttcacc acaattaata ccagcggcgc agaaacctga cgcaataacg ccagcgcgtt
    29881 gagatcgtaa agcgcggaga gatcgcccac aatcgccagc gtcggtttgc cgcttgcccg
    29941 ctgaacgcct gcggcggtcg aaagcagtcc gtcgataccg ctggcccctc ggttgctgta
    30001 caccgggtaa cctgccggaa gttgcgaaag cgcatcaatc agacgtacca ccaggctgtt
    30061 accaacaaac aattgcccct gttcaggcag atagtcgctg atgcgatgcg ccagttgcgc
    30121 ttcgccaaac gcatcacggc gggcaataac cgcctgcatt gcctgttccg ccaggcgcgg
    30181 gatttcaacg caccagggct ggcgtttttc tgccggatgc agctccagcc agtcggcaat
    30241 attggcaatt aagcgacgtc cgcgatggtg tgccggatca agtcgccctt caatgtcatc
    30301 aacaatccag tactcttctg gttcacagct tgcctgccat tgcaggagcc gtttccccgt
    30361 caggctactt cccagttgca ccacaatttg cgcctgctgc agctcgctgg tcgctttggc
    30421 attgcctaac caaagatcgg cacacggcag cggctgcccg gtttgtgaca gcacatcgcc
    30481 aatcagcggc cagccaagag tttgcgccca cagggcaact tttttgccct cttccgcact
    30541 catgcgcccg gcaaccacca cgccgcgctt ttgtcgccag aagaaccagt cgcgctgttt
    30601 ttcactttcc agacgaggcg cttcacgcag ccacggtttg tcgtcctgcc accagtcacc
    30661 cagacgctgt tgccagctaa gcccggtatc gtccatttcg ccatacagcg gttcagcaaa
    30721 cgggcagttg atatggactc cccccgcatg aagcgtaccg agagcgtggt cgatggtaga
    30781 aaccagccaa cgtgcgggga tatcctgggt cgggcgcggc aatgaaatac tgtgcgtggg
    30841 gtgagaggcg aacattcccg gctggcgaat tgcctgattc gcgccgcagt caattagctc
    30901 cggcgggcga tcggcggtta agagaatcag tttttctccg gttaacccgg cttcaatcag
    30961 tgccggatag agatttgcca ccgccgtgcc ggaggtcaca atcaccgcca ccggctgctt
    31021 gctgactttc gccagcccca gcgccagatg ccccaaccca cgctcatcga aatgggtgtg
    31081 gtgaatgaat gcggaattct ccgccgccgc taacgttaac ggtgtagaac gcgagcctgg
    31141 ggcgatacag atgtgtctga cgccgtgacg cgttaatgct tccagaatga ccgccgccca
    31201 gcgtcggtta aatgcgctta ctgacatgag tttgtccggt atcaatattg cggctaagta
    31261 taaggagcta caaaaataga ttattgatat gaatcggtaa tgatgcgact cattactatt
    31321 ccatttgtaa taaagtacgc agccctgccg ctttgttgtc gatttcctgc cactcttgct
    31381 cggggtcgga accacggaca atgcccgcgc cagcatataa tcgcacgaca ttgccgctaa
    31441 tttttgctga gcgcagggaa acgcagaatt cgctttgttg taatgagaga tagcccgcag
    31501 aaccggcgta ccattctcgg gtgaacggtt cgtgacgggc gataaactgt cgcgccagat
    31561 cgcgcggtaa gccagcaact gctgccgttg gctgcaactg atgtaaacag atcacatcat
    31621 ccgctttgtt gagtgaagtc cagatacagc ggcgaagatg ctgcacttta cgcagacgca
    31681 gtacctgcgg cggtaaaaca tccagcgtct gggtatcggc ctgtaatcgt tggcagatat
    31741 cttccaccac cagcatgttc tcgcgctggt ttttatcatc cgccatcagc cactctccta
    31801 actgctgcgc ctgcttatca tcaggatgat ttgctactgt tcccgccagc gcttcagtac
    31861 gcagcgcttt gtcacgccgc cgccataacc gttccggtga agagccaaga aaagcatttt
    31921 cgccatcaaa ggccatgtaa aaatggtagc aattcagatt cagtcgacga ctggcagcca
    31981 tcatcgccgc cgcgttgacc ggacttgcga aatgcaggtc agttgcccga gcgagcacca
    32041 ctttgtcgag ctcaccttcg gcgatggttt tcgttgccag ttcgattaat tgcgtccagc
    32101 ccgttttgtc cggccagtgt tgttctcgcg tggtggttaa atgtaaccca ggcaagggct
    32161 tgatactcac cagtgtggcg ataaattctt ttgcctgaat cgcatcgtgc tgaagggagc
    32221 tttcgctgaa tagcgtcagc cgcagcgtgg ctttaccgcc acagcgtcgc cattccaggc
    32281 ggggtaaaag taaattgccc tgcgacgggt caaaagcatt cagcccccaa atgcgtaagt
    32341 cagcgtgttc cgggtgctgg cgaagaaaac gttgtgcctg gtccaacgac gtaaaacggg
    32401 taatcgcgcc caggacggca gcttcttcat caccattacg ttgttgccag tagaattgcg
    32461 ggtacgtttg ctgactggcc agccagctca aggcatcaaa agcgtctttg agagggaaag
    32521 gaatatcgat aacccgaatg ccgggtgtcg ccggaatctc ttgcgacaaa tggcgcagta
    32581 gattttccag cgccgtagta agtgattgca cgcggacctc tccctgttaa aagcctcaca
    32641 ttatacgggg tactacaaaa aaaatgcagt accccggtgt agggaggttt taacggcgtg
    32701 ccagcaacag tcctagtacc agcccgacgg ccgcgcccac accaattcct tgccagggtt
    32761 tttcgtggac gtagtcatca gcacgataaa cagcctgctt cgcccgatag taataactgt
    32821 ctgaagcctg gctaacccgt tttttcacat catccagcgc tttttctgca tgcgctttca
    32881 gctcaacata tttctgatcg gcgggatcgc cagaggagcg gagcacctct tccagtgttt
    32941 cactaagcag cgtcaggtcg tcatcgatac gtgtatcacc aaactgatta gacattctcg
    33001 ttctccattt gcgtaaaacc tgtcactaac tatagacaag agttgttgat tacgcctgaa
    33061 ttacctcgcg tgccatccca atgtgtggga taccatcttc ttcatagacc tccgtcacag
    33121 ggataaaacc gaaactctgg tagaagtttt gcagatgcgc ctgcgccccg aggtaaacag
    33181 gcttatcagg ccagtgatgc gtacatgttt cgagtgtttt actcatcagc tgctgcccca
    33241 ctttttcacc acgcaacgct tcactgacaa tcacccgacc tataacgacc ggctcaagat
    33301 catcatcact tttcagaatc ctcgcatacg ccaccagttc atcatttttc caaccgagaa
    33361 tatggcgatt atcccccgtc aaatcatcac catcgatatc ctgataaggg caattctgct
    33421 cgacaacaaa caccgcgcaa cgcagttgta ataaggcata aagctgggaa accgataatt
    33481 cagagtgatg cagatcttgc cattcaatca taatgctctc cttttagatg tatgccttat
    33541 actagccgga atattttttg aaacgtgatg aactcatgga attaattttt ttaggtactt
    33601 cagccggtgt gccaacccgc acgcgcaatg tcacggcaat attgcttaac ctgcaacatc
    33661 cgacccagag cggactttgg ttgtttgact gcggtgaagg cacccagcat cagctactgc
    33721 ataccgcctt taaccctgga aaactggaca agatttttat cagtcacctt catggcgatc
    33781 atctttttgg tttacccggc ttgctgtgca gtcgttctat gtcaggcatt atccaaccct
    33841 taacgattta tggtccgcaa ggtatccgtg aatttgtgga aaccgcgctg cggattagcg
    33901 gctcatggac cgattatccg ctggaaattg tcgaaattgg cgctggcgaa attctcgatg
    33961 atggcctgcg caaagtaacc gcttatccgc tggaacaccc actggaatgt tatggctatc
    34021 gtattgaaga acatgataaa ccgggtgcat taaatgccca ggcattaaaa gctgctggcg
    34081 tgccgcctgg cccactgttt caggaattaa aagcgggcaa aacaatcacg ctggaagatg
    34141 gaaggcagat taacggcgca gattacttag ctgctccagt gccaggtaaa gcgctcgcta
    34201 ttttcggcga taccggcccc tgcgatgccg cacttgacct ggctaaaggt gtcgatgtca
    34261 tggtgcacga agcgacgctg gatataacca tggaagccaa agccaatagt cgcggccata
    34321 gctctacacg ccaggctgcg acactagccc gtgaggctgg agtcggcaag ctaatcatta
    34381 cccacgtcag ctcgcgctat gatgacaaag gttgtcaaca cctgttacgt gaatgcaggt
    34441 caattttccc ggcgactgaa ctggcgaatg atttcaccgt gtttaacgtt taactctgct
    34501 tattattaaa aacagggtga aacttgccct gttatagcaa cccgcgctac atatatccac
    34561 ccacacaggc atattgagcg atttttgata tttattcatc tccttacgca ctatattcag
    34621 agcaataagt tgcctctttt catcttgatt cagcacaacc tttgcaacta aaaggagccc
    34681 atgatggtta cagttgtcag caattattgt caattatctc aaacccaact cagtcagaca
    34741 tttgcagaaa aatttactgt gaccgaggaa ttactgcagt ctttaaaaaa aacagcgtta
    34801 tccggagatg aagaaagtat tgagttactg cataatattg cgttaggtta tgatgaattt
    34861 ggaaaaaaag ctgggtaatg ctgccaactt actgatttag tg
//
LOCUS       DAJTRD010000034        34801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTRD010000034 DAJTRD010000000
VERSION     DAJTRD010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.034"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..2099
                     /locus_tag="OO315_001847"
     CDS             <1..2099
                     /locus_tag="OO315_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5890780.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2096..3184
                     /locus_tag="OO315_001848"
     CDS             2096..3184
                     /locus_tag="OO315_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5890781.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3272..4570)
                     /gene="exuT"
                     /locus_tag="OO315_001849"
     CDS             complement(3272..4570)
                     /gene="exuT"
                     /locus_tag="OO315_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5890782.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5055..6467
                     /gene="uxaC"
                     /locus_tag="OO315_001850"
     CDS             5055..6467
                     /gene="uxaC"
                     /locus_tag="OO315_001850"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5890783.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6482..7969
                     /gene="uxaA"
                     /locus_tag="OO315_001851"
     CDS             6482..7969
                     /gene="uxaA"
                     /locus_tag="OO315_001851"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5890784.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8052..8603
                     /locus_tag="OO315_001852"
     CDS             8052..8603
                     /locus_tag="OO315_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5890785.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8608..9852)
                     /gene="sstT"
                     /locus_tag="OO315_001853"
     CDS             complement(8608..9852)
                     /gene="sstT"
                     /locus_tag="OO315_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5890786.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10448..11413)
                     /gene="alx"
                     /locus_tag="OO315_001854"
     CDS             complement(10448..11413)
                     /gene="alx"
                     /locus_tag="OO315_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5890787.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11684..12682)
                     /locus_tag="OO315_001855"
     CDS             complement(11684..12682)
                     /locus_tag="OO315_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5890788.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12761..13453)
                     /locus_tag="OO315_001856"
     CDS             complement(12761..13453)
                     /locus_tag="OO315_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5890789.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13530..14033)
                     /locus_tag="OO315_001857"
     CDS             complement(13530..14033)
                     /locus_tag="OO315_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5890790.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14118..15254
                     /gene="rlmG"
                     /locus_tag="OO315_001858"
     CDS             14118..15254
                     /gene="rlmG"
                     /locus_tag="OO315_001858"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5890791.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15539..15853
                     /gene="higB"
                     /locus_tag="OO315_001859"
     CDS             15539..15853
                     /gene="higB"
                     /locus_tag="OO315_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5890792.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15850..16266
                     /gene="higA"
                     /locus_tag="OO315_001860"
     CDS             15850..16266
                     /gene="higA"
                     /locus_tag="OO315_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5890793.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16311..18329)
                     /gene="fadH"
                     /locus_tag="OO315_001861"
     CDS             complement(16311..18329)
                     /gene="fadH"
                     /locus_tag="OO315_001861"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5890794.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18555..20906)
                     /gene="ygjK"
                     /locus_tag="OO315_001862"
     CDS             complement(18555..20906)
                     /gene="ygjK"
                     /locus_tag="OO315_001862"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5890795.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20923..21342)
                     /locus_tag="OO315_001863"
     CDS             complement(20923..21342)
                     /locus_tag="OO315_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890796.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21320..21673)
                     /locus_tag="OO315_001864"
     CDS             complement(21320..21673)
                     /locus_tag="OO315_001864"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890797.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21674..21994)
                     /locus_tag="OO315_001865"
     CDS             complement(21674..21994)
                     /locus_tag="OO315_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890798.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22128..23561)
                     /locus_tag="OO315_001866"
     CDS             complement(22128..23561)
                     /locus_tag="OO315_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5890799.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23624..24073)
                     /locus_tag="OO315_001867"
     CDS             complement(23624..24073)
                     /locus_tag="OO315_001867"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5890800.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24070..27156)
                     /gene="ebgA"
                     /locus_tag="OO315_001868"
     CDS             complement(24070..27156)
                     /gene="ebgA"
                     /locus_tag="OO315_001868"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5890801.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27339..28322)
                     /gene="ebgR"
                     /locus_tag="OO315_001869"
     CDS             complement(27339..28322)
                     /gene="ebgR"
                     /locus_tag="OO315_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5890802.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28540..28871
                     /locus_tag="OO315_001870"
                     /pseudo
     CDS             28540..28871
                     /locus_tag="OO315_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28913..30292)
                     /gene="ygjG"
                     /locus_tag="OO315_001871"
     CDS             complement(28913..30292)
                     /gene="ygjG"
                     /locus_tag="OO315_001871"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5890803.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30710..32230
                     /gene="aer"
                     /locus_tag="OO315_001872"
                     /pseudo
     CDS             30710..32230
                     /gene="aer"
                     /locus_tag="OO315_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32384..33007)
                     /gene="nfeR"
                     /locus_tag="OO315_001873"
                     /gene_synonym="yqjI"
     CDS             complement(32384..33007)
                     /gene="nfeR"
                     /locus_tag="OO315_001873"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5890804.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33284..34029
                     /gene="nfeF"
                     /locus_tag="OO315_001874"
                     /pseudo
     CDS             33284..34029
                     /gene="nfeF"
                     /locus_tag="OO315_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34083..34158)
                     /locus_tag="OO315_001875"
     tRNA            complement(34083..34158)
                     /locus_tag="OO315_001875"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34123..34125),aa:Ile,seq:cat)
     gene            34283..34789
                     /gene="mug"
                     /locus_tag="OO315_001876"
                     /pseudo
     CDS             34283..34789
                     /gene="mug"
                     /locus_tag="OO315_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8489 a         8949 c         9144 g         8219 t
ORIGIN      
        1 atttcaacgg ttatgatgca gatgatgtca gtgacagtaa tactgacgtc agcgatctct
       61 attatcgtta tgatttttta cgtcgttatt atgtgcaggc gggtcgcatg gacaaccgca
      121 cactatttaa tgcacaaggc gggaacttta cctttaactt tctgccactc ggtgcaatcg
      181 atgggatgcg tatcgggtcg acactgagct atttaaacca ggcgcaaagc cagcaaggaa
      241 ccccagtaat ggttctgctt tcgcgcaatt ctcgtgttga cgcttatcgt aatgagcaac
      301 ttttgggatc gttttatctc aatagtggtt cgcaatttat tgataccagt tcctttccgc
      361 caggcagcta cagcgtggca ttaaaagtct acgaaaataa ccaactcacc cgcaccgagc
      421 ttgtgccgtt taccaaaacc ggcggtctga ctgacggaaa tgcgcaatgg ttcttacagg
      481 caggtaaaac tacatcacag gtttctgatg atgaaagttc agcttatcag ctaggagtac
      541 gcctgccatt acatccgcaa tatgagctct acgcagggct ggcgaatgct gatgatgtga
      601 gtgctttcga gttaggtaat aactggacgg cagatttagg cagggtgggg aatcttgcaa
      661 tcagcgccag cgtgttccgt aacgatgacg gcggcaaagg tgatatgcaa caggccaact
      721 ggagtaatcc gggatggccg acgttgggct tttatcggac caactctgac ggcgatgctt
      781 gtacaaccga cagcagagag agctataacg ccttaagctg ttatgaaagt atttccgcga
      841 cagtttcact gaattttgtc ggctggaata tgatgctggg ttatacctgc acacaaaata
      901 acactgatga tagtttgcgt tgggataaac agcagagctt tgaaaataac tatcttcgcc
      961 agacaacagc gcaaagtatc tctgaaactg tacaacttag cgcttcccgc gctattgtga
     1021 tgcgtgactg gattttgagt acgtcagtcg gtgttttcca tcgtaatgac aacggtggcg
     1081 ataacgacga caacggcttg tacttatcgt tttcgttatc tgacacgcca acgatggata
     1141 gcaataacaa cagccattca accaatgtgc ctacggatta tcgttatagc gaacaggatg
     1201 gcgatcaaac gtcatggcag ttatcgcata ccttttataa cgattcattc agccataaag
     1261 aacttggcgt aaccgtcgga ggcctgaaca ccgataccat aaacagcgcg gttaacgggc
     1321 gttgggatgg gcaatacgga aatgtctacg ctaccgtatc tgatagttat gaccgtaaga
     1381 atcatgatca tctttcggcc tttacgggca cttatagctc tacgctggct gtcagtcgct
     1441 atggcgttaa tttgggagcc agtggtacag acgatttgct gggtgcggta ctggtggatg
     1501 tgaaaggctt ctctgaacag gatgaagaga gtcaggatct gcaactcgaa gcgcgggtgg
     1561 caggcagccg aactttgcag cttggtcaaa gtgacagtgt gttgttccct tatcctggat
     1621 ttcagtctgg ttttgttgag gttaacgaca gtagccaggg caatcagcag gggacaacaa
     1681 acatcattaa cggtgcaggg aatcgtgaat taatgttgtt gcctggcaag ctgcgctatc
     1741 gcgaagtgtc tgccagcttt aactacaact atatcggtcg tttattactg ccggcagcgg
     1801 tgaaaaaatt cccgattgtg gggctgaaca gcgccatgtt actggtagct gaagatggcg
     1861 gatttacact tgaaattaac ggcagcgaaa aagagctcta tctgctttcc gggcagcaat
     1921 tccttaagtg tccactgagt gttgtaaaga aacgcgccag cattcgttac agcggagatg
     1981 ttacttgtag tgtggtgact tattcacaat taccggagtc gattcaggtt caggcacagt
     2041 tgaaacagcc taaattacgt ggaaacgttc agacggcgca aagggaggtt gcaccatgag
     2101 aaatcgattg attgcggcga tattgggctt gtttggcacg ctcactggcg ttcaggcagc
     2161 tcctgacgtg accagtgaaa ttacgtatga tttggcatct ggtagagcgg attattactt
     2221 ctggaaggat gaagcgtcgg caggaaataa tggatatatg tggtatgaat gttcgtatcc
     2281 tgacctccaa caaacctgta cagctaatgg aaatatatcg acagtacaaa tctatttaac
     2341 tgaacaacgc agtgggatgc gttggccggt aaaactcaaa ggatttaaaa cagccattgt
     2401 aagtagcgat gaagcgccgc taggatgcaa ggggggcaaa gggcttcaga cgaatcttaa
     2461 ggattctaat agatcttcat gtacagaaga tggtcaacat tattatatat acgatacaaa
     2521 gtttcttacg ctctaccttg agcagacaga gatgaagaat ttgccgattg gtggcgtctg
     2581 gaaggggaaa gttaaattac attcgaacag cccggcccag gactatttcg caaatattac
     2641 cctgaatacg ctcgacccca accatattga cgtgttcttc ccggagttcg cccacgccac
     2701 gccaagggtg cagttagact tgcatccaac aggaagcgtt aacggcagca actacgcgca
     2761 agatctgacc atgttggaca tgtgcctgta cgatggtttt aacggtaatg ccatcagtta
     2821 tgaaatcatg ctcaaagatg aagggcgacc agctgcaggg cgcagagacg gttacttctc
     2881 tatctatcgt cagggaggga ccaccaccga cgagggagaa cgcattgatt accgggtcaa
     2941 aatgtacaac ccggaaaccg gtgggcaaat tgatgtgcgc aataatgaaa atatggtctg
     3001 gaacagcatt aacctgaaac gtgtgcgtcc ggtggtactg cccggtattc gctatgccgt
     3061 gatgtgtgtg ccgacaccct tgactctggc agtcgataaa ttcagcgtga tggataaaca
     3121 ggccgggtac tacatgggca aattgtcagt aatctttacg ccttccttgc caaccatcaa
     3181 ttaaccctgc aaaccggaca aacgcttttt tctatataaa cgacgaagcc gcctgataag
     3241 gcggcttcgt cgttataact tacgttaaac gttaatgttg cggtgcggga tcgttatgtg
     3301 tttcctgcgc cacctcaatc gccggtttgt tctgcaatac ggtccagata accagcgcac
     3361 ccagcaggtc aaatactgcc agaactgcga acagcgggct gaagccgatg gtgtcagcca
     3421 gtgcaccgac aaccagcgca aacagcgtac ttgccagcca tgcggacatc ccggttaaac
     3481 cgttcgccgt cgccacttcg ttacgaccga acacgtcaga agagagcgta atcagcgcgc
     3541 cagacagtgc ttggtgggca aaaccaccga tacacagcag cataattgcg acatacgggt
     3601 tggtgaacag accgatcata cccgggccaa tcatcagcac agcacccagc gttacgacca
     3661 tcttacggga aacgatcagg ttcacaccaa accaacgctg gaacagcggc ggcaggtaac
     3721 caccgaggat acaaccgagg tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct
     3781 ctttcaggtt aaagccgtaa actttaaaca tgaacagcgg gatccacgcg ttaaaagtac
     3841 cccaggccgg ttctgcaagg aaacgcggca gtgcgatacc ccagaactga cggttacgca
     3901 ggatctgacc aacggacatt ttcttcgccg tgctcacctg gtgctgggct tcctgaccat
     3961 taataatata gtcgcgttct tcatcggtca gatgcttctg gtcgcgcgga tgtttataga
     4021 aaatcagcca tgccatcgcc cagataaagc tcaatgcacc ggagatgata aatgccatct
     4081 gccagctgtg catcacgatt gcccatacca ccagcggcgg cgcaatcatc gcaccaatcg
     4141 aagaacctac gttaaagtag cctactgcga tggaacgctc tttcgccggg aaccattcgg
     4201 agctggcttt cagacccgcc gggatcatcg ctgcttccgc ggcaccgacc gcaccacgag
     4261 caacagctag gccaccccag ctacctgcca gcgcggttgc accacagaac acggcccaca
     4321 gcacagcaaa cattgcataa ccgattttcg tacccagcac atccagtaca taacctgcta
     4381 ccggttgcat gaccgtataa gcagcagaat aggctgcgat gatataggaa tactgttggg
     4441 tggagatgtt taactcttcc atcagagttg gcgcagctgc cgccacagtg ttacgcgtca
     4501 ggtaaccaag cacggtgccg agcgtcacca gtgcgatcat ataccaacgt aaccctttaa
     4561 ttttacgcat gtaaaacctc atcgttttgt tatatccgca ggaagcgcgg ccatcatccc
     4621 gccagaggct gaagatgtta gttacgaccg ggtaaccgaa aaaaaaaacc ggcacgcccc
     4681 gaacgttgcc atgaaagttt cgtgctaatt cggcttccgt accggttaac ccgattcatc
     4741 taacaaatga atgaatcggc aatttgcatt ccgtcgactt atgatttgcg acggcagaaa
     4801 gataacttgt catacaacct taaaaggtga gagccatcac aaatgtgcga atatttgtag
     4861 ggacattacc tgacgacagc gaggccagta ctggcgcggc ctgcagcgag atttaccact
     4921 ttgagagtaa tttttttaac tacgtttatt gatctaactc acgaaaatat cttcggactc
     4981 tggaaattgg tgtgataact ttgtcagcat cgcaccataa gcaagctagc tcactcgttg
     5041 agaggaagac gaaaatgact ccgtttatga ctgaagattt cctgttagat accgaatttg
     5101 cccgccgtct gtatcacgac tacgcaaaag accagccgat tttcgattac cattgccatt
     5161 tgccgccgca gcagattgcg gaagactatc gttttaaaaa cctgtatgac atctggttga
     5221 agggcgatca ctacaaatgg cgcgctatgc gtaccaacgg tgtggctgag cgtctgtgta
     5281 ctggtgatgc gtctgaccgt gaaaaatttg acgcctgggc ggcgactgtt ccgcacacta
     5341 tcggcaaccc gttataccac tggacgcacc tcgaactgcg tcgtccgttc ggtatcactg
     5401 gcaaattgct ttctccgtca actgccgatg aaatctggaa cgaatgtaac gaactgctgg
     5461 cgcaggataa cttctccgcg cgcggcatca tgcagcagat gaacgtgaaa atggtcggca
     5521 ccaccgatga cccgatcgat tctctggagc atcacgcaga gatcgccaaa gacggctctt
     5581 tcaccatcaa agtgctgccg agctggcgtc cggacaaagc cttcaatatc gaacaggcga
     5641 cctttaacga ctacatggcg aagctgggcg aagtttccga taccgacatt cgccgctttg
     5701 ctgacctgca aactgccttg accaaacgtc tggatcactt cgccgctcac ggctgtaaag
     5761 tgtctgacca tgcgctggac gtggtgatgt ttgctgaagc gaacgaagcg gaactggaca
     5821 gcattctggc gcgccgtctg gctggcgaaa ccctgagcga gcacgaagtg gcacagttca
     5881 aaactgcggt actggtgttc cttggtgccg aatatgcacg tcgcggctgg gtacagcagt
     5941 accacattgg cgcgctgcgt aataacaacc tgcgtcagtt caaactgctg gggccggatg
     6001 taggctttga ctccatcaac gaccgtccga tggcggaaga gttgtctaag ctgctgagca
     6061 agcagaacga agaaaacctg ctgccgaaaa ccattctcta ctgcctgaac ccgcgcgata
     6121 acgaagtgct gggcaccatg atcggtaact tccagggcga aggtatgccg ggcaaaatgc
     6181 agttcggttc cggctggtgg tttaacgatc agaaagacgg tatggaacgt cagatgaccc
     6241 aactggcgca gctcggtctg ctgagccgct ttgtcggtat gctgactgac agccgtagct
     6301 tcctgtcata cacccgtcac gaatacttcc gccgcattct gtgccagatg atcggtcgct
     6361 gggtggaagc gggcgaagca ccggcggaca tcaacctgct gggcgagatg gtgaaaaata
     6421 tttgctttaa caatgcgcgt gactacttcg ccattgaact gaactaaggt ctggggttga
     6481 tatgcaatac atcaagatcc atgcgctgga taacgtcgcg gtcgctttag cagatttggc
     6541 tgaaggcaca gaagtcagtg tcgataacca gactgttacg ctgcgccagg atgttgctcg
     6601 tggacataaa tttgcgttaa cggatatcgc aaaaggggcc aacgtcatca aatatggcct
     6661 gccgattggt tatgcattgg cggatattgc ggcgggggaa cacgtccacg cccacaatac
     6721 gcgcacgaat ctgagcgatc tggatcagta tcgctatcaa cttgattttc aggatctgcc
     6781 tgcgcaagcg gcagatcctg aagtgcagat ctatcgtcgc gctaacggcg atgtcggggt
     6841 gcgtaacgag ctgtggatcc tgccaaccgt gggctgtgtc aacggcatcg cgcggcaaat
     6901 ccagaaccgt ttcctgaaag agaccaacaa cgccgaaggt accgacggcg tgttcctctt
     6961 cagccacacc tatggctgct cacaactggg cgacgatcac atcaacaccc gcaccatgct
     7021 gcaaaacatg gtgcgccacc cgaacgcggg cgcagtgctg gtgattggtc tgggctgtga
     7081 aaacaaccag gttgccgcat tccgtgaaac gttgggcgat atcgatcctg aacgcgttca
     7141 tttcatgatc tgccaacagc aggatgatga gatcgaagcc gggatcgagc atttgcatca
     7201 gctgtataac gtgatgcgca acgataaacg cgagccaggc aaactcagcg aactgaagtt
     7261 tggtctggag tgcggtggtt ctgacggtct ttctggtatt actgctaacc cgatgctggg
     7321 gcgtttctct gactacgtga ttgctaacgg cggtactacc gtactgaccg aagtgccgga
     7381 gatgtttggc gcagagcagt tgctgatgga ccattgccgc gacgaagcaa cgtttgaaaa
     7441 actggtcacc atggtcaacg acttcaaaca gtactttatt gcccatgatc agccgatcta
     7501 cgaaaaccca tcaccgggga acaaagcggg cggtatcacc acgctggaag acaaatcact
     7561 tggctgtacc cagaaagcgg gttccagtgt cgtggttgac gtgctgcgtt acggcgagcg
     7621 tctgaaaacg ccagggctga acttgttaag tgcgccgggt aacgatgccg tagcgaccag
     7681 cgccctggcg ggtgcgggct gccatatggt gctgttcagt actggtcgcg gcacgccata
     7741 cggtggattt gtgccgacgg tgaaaatcgc caccaacagt gaactggcgg cgaagaaaaa
     7801 acactggatc gactttgacg cgggtcagct gatccacggt aaagcgatgc cgcagttgct
     7861 ggaagaattt atcgacacca tcgttgagtt tgccaacggt aagcaaacct gcaacgagcg
     7921 taacgacttc cgcgaactgg cgatcttcaa aagcggcgta acgctataaa gtcgaaatga
     7981 gttgttaata aaaaacggcg tttcataatg tgaagcgccg tttgcttttc catacctgct
     8041 aaggatttat catgaccgcg tattggctgg cccagggcgt gggtgtcatc gcctttctga
     8101 ttggtatcac aacatttttc aatcgtgacg aacgtcgctt caaaaagcag ctttcggtct
     8161 atagcgccgt tattggcgta catttttttc ttctgggcac ctatcccgct ggtgccagtg
     8221 ccatccttaa tgccattcgt acattgatta ccttacgcac gcgcagctta tgggtaatgg
     8281 cgatttttat tgtgctgact ggcggaattg gcctcgcgaa gttccatcat cctgtcgaac
     8341 tattgccggt tatcggtacg attgtcagta cctgggcgct gttccgctgt aaagggctca
     8401 ccatgcgctg cgtgatgtgg ttttcaacgt gttgctgggt gattcacaac ttctgggcgg
     8461 ggtcgatagg cggcacgatg attgagggta gttttctgct tatgaatggc ctgaatatca
     8521 ttcgtttctg gcggatgcag aaaaggggaa ttgatccgtt taaagtagag aaaacccctc
     8581 ccgccataga cgaaaggggt taaacaatta attacgcagg gcgctatttg ccagacgatc
     8641 gtcttccgcc tggcaagctg ccgcagtgaa cagcacgtca gttgaagagt tcagcgcggt
     8701 ttcgcaagag tcctgcaata cgccgataat aaagccgacg gcaaccacct gcatggcgat
     8761 atcgttcgaa ataccgaaca tattacaggc cagtgggatc agcagcaaag aaccccccgc
     8821 cacgccggat gcgccacagg cacacagaga agccactacg ctcaacagca gcgctgtggg
     8881 cagatcgacc ggaataccca gcgtattaac cgcagccagc gtcaacacgg taatggtgat
     8941 tgctgcgccc gccatattga tggtggctcc cagcggaata gaaactgaat aggtatcgcg
     9001 atccagattc agcttttcac acagcgccat attcaccgga atgttcgctg cagagctgcg
     9061 ggtgaagaag gcatacacac cgctttcgcg caggcacagc agcaccagcg ggaacgggtt
     9121 acgacgaatt ttccaccaca ccagcaatgg gttaaccacc agcgccacca gtaacataca
     9181 gccaaccagc acgaccagca gttgcgcgta gccccacagt gtggagaaac cggtggttgc
     9241 cagggtagaa gaaaccagcc caaaaatacc gaacggtgcg aagcgaatga ccagtttcac
     9301 cataaaggta acggcattcg acatatcatt gaccaggttt ttggtggtct cgttaccgtg
     9361 acgcagtgcg aagccgaggc cgatcgccca caccagaatc ccgatgtagt tacctttcag
     9421 cagggcgtcg atggggttgg aaaccatgct cattaccagt ccgcgcatca cttcgacaat
     9481 gcctgacggc ggcgaaatat cacccgcgct actggacaag tgcagggtag aagggaaggc
     9541 aaagctgaag actactgcgg ccagagctgc agagaaggtg cccagcagat agaggaacaa
     9601 aataggacgg atattggttt tctgcccgtg ctggtggtta gcaatagatg ccattaccag
     9661 catcaacacc aggatggggg caacagcttt cagtgcgccg acgaacaaag tacctaacag
     9721 accaacagct tccgccgcgg gttttgagat ccatgccaga agaatcccca gaacaaggcc
     9781 gaccaggatt tgttttacca ggctgccatg agccagacgc cggaatagcc ccggtgaacg
     9841 ttgcgtagtc atttttcgat cctttcattg tgttgtcgca catccctggt gcgttctgtc
     9901 gacgcatctt tcaggatgta acaaagtgtt tgcaaggtcg agtataagga aagttacagc
     9961 ttcgggaagc gaaaaatgct ggattttaaa gagaggtcat gttttgcaac ttaatggttg
    10021 catttattta acataaacag agggaaggac tggctactaa ggctgattag ataacatatg
    10081 ggcaaaacta tttggaatat gagaacggtt gcgctccttt aacgtgttat gccatataaa
    10141 gtcactggcg cagggagcgc gcagggggcg gccaatcgcc gccgccccct gcacccccag
    10201 gctctggcga acaaaatcgc cgctgcgcgg ttccctcagc ctatcccttg cggctaccgg
    10261 gtcgggcgca aggtaacgtc cctgtaagat gcgccctcgc cccggcctcc agggaacgct
    10321 tcggcgattt tgacgccacc aacacccgtg cggtcttttt gtaaagagct ataagccgat
    10381 tacataatta atagctcacc tttgtctgga atgagttatc tctaggaatg gcttaatata
    10441 cagcgaatca ttctccccgc tgcttatcat gccgataatt cacccaggcg ttgataataa
    10501 acgtcatcac cagaatgcca aacaccacgc ccagcgagac ggcgattgga atatggtaga
    10561 agtcgacaat cagcatcttg ataccgataa acaccagaat caccgccagg ccatatttga
    10621 gcatcgagaa acgctctgct acgcccgcca gcaggaaata catcgcacgc aggccgagga
    10681 ttgcaaacag gtttgaggtc agcacaatga acgggtcagt ggtcacggcg aagatagccg
    10741 gaatgctatc cacggcaaaa atcacgtcgc tcaattccac cagaatcagt accagcatca
    10801 gcggtgtggc gtacagcaat ccattcttac gcacaaagaa atgctcgttg tcgatggtgt
    10861 cggtcatgcg caaatgaccg cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt
    10921 catcttcatg ggcgagcgcc atcttcacgc cggtaaacag caggaaggca ccgaagatat
    10981 acaggatcca gtcgaactgc gaaatcagcc agctgcctgt gaagatcatg atggtacgca
    11041 gaacaatcgc cccgagcacg ccatacacca gcacgcgacg ttgtaatgcc gccggaacgg
    11101 agaaatagct gaacaacatc agccagacaa agacgttatc gaccgccagc gatttctcaa
    11161 tcagataacc cgtgagaaag gccagggcct gtggatcggc aacggcgcga ccttcggttt
    11221 gcaccagata ccaccagaaa gcggcgttaa acagtaacga cagcgtcacc cagaccagcg
    11281 accaggccgc agcctgtttc atggtcatgg catgtgcccc acgacgcccc tgcaacaaca
    11341 ggtcgatagc cagcataatg gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc
    11401 cgacagtatt catagaagtt ccttacacat aaaaaaacgg ccaacgtcgg aagacgtcag
    11461 ccgctgcttt ttatgcatag acctcgcctt ccggcaaggt ctcacttaca acaaaaaagg
    11521 aatacgtctg cgtattcctt tcgggttgcc cggtgaccgg atgtggtttt tcactcatcg
    11581 taatgacgat cgaccggcaa tgaagttact cccctttgca ggtaacaaag tatgacagac
    11641 cattcagtca gtcaatggtc tgttacatac cttttacact gttttacttt ggcaagtata
    11701 gttttacgct atctgccgga aaaatcaccc cagtttggcg gcggatctcg gtcagcagtt
    11761 tggctgtgat gcgactgacc gccagccctg gatgatccac cagatgctca tccaccaggg
    11821 tagcgaacag ctctgcttcg tagagcatgg tattaatatg ctgcggctgg gtgagatcct
    11881 gcatctggct gccacgcggc acaaagcaga ctttctggca ttcagacagt ttttcaatca
    11941 ccagcgatcc cgcttcgccc tgaatctcgc ttcccaggac agaatcactg acctttgagt
    12001 gctgcaaggt gacgctgaaa tcaccgtaat ccatcaccac cacgccgtgg gcgtcaacgc
    12061 cgcttgccag caaactcgcc gttgcctgca cgcttttcgg ctcaccaaat aacgccaccg
    12121 ccgacgccag gcagtaaaag ccgatatcca taattgaacc gttagagaat gccggattaa
    12181 aggtgttggg attctcaccg tccaggtaac gttgataccg cgaggaatac tggcaatagt
    12241 tgaaaaagac tttacgcagt ttgcctgctt tcggcagcgc ctggcgcaac aaatgaaagt
    12301 tcggcaggca ggcggtttta aatgcctcaa acagcaccac ctgattttcg cgcgcacagg
    12361 caatggcggc atccacttcc gccagattcg acgccagcgg tttttcgcaa atcacatgaa
    12421 ttttatggct aaggaaaagt tgtgtctggg aaaagtgcag ggaattcggg ctggcaatat
    12481 acaccgcgtc aatggcatcg ctttccgcca tcgcttccag cgacgtaaac agatgctcga
    12541 cagaaaaatc attggcgaag tgctgagcct gctcaaggct gcgggaatat acggcggtta
    12601 acttgtattt accgctctca tgggcggcct cgacgaactg gcgagtgatc cagttcgtac
    12661 caatcacagc gaaacgtatc ataacgcgtg gatactccat aaagggattc tgtcgtcaat
    12721 gtagcacgcc tttttcatct tcgacgtgcg cagatacgca ttattctttc gccggacagc
    12781 catgctcact aaacggccca atcatgaccg atggcccgag atatttcggc tggcgatgga
    12841 ggatttttgc gtcaatcacc gcgctgacac gagcgagttt ctcccgcagt tgagtataaa
    12901 aaccgtgctt catattgagg tctggcgcgt taaacccgat agcgtcgatg ccgtactgtt
    12961 ttgccagcca gatggtgcgt tggttatgaa attcttgcga gataatggtg atatggtttt
    13021 ccccaaacac ctttttggca cgcactaccg aatccagcgt tgagaatccg gcatagtcgc
    13081 aaaagatcac tttcgccggt acgcctttag cgatcaaagc ctgctgcata cctgatgctt
    13141 catcgtaatt tttacgtccg ttatcgccac tcaccagtag ccatttcact tttccggcgt
    13201 ggtacaacgc tgctgcggta tcaatacgtc gggtaaagta gcgattaccc ggcctcgccc
    13261 ccagcaataa acccacgtta cgcgccggga cggcgttgac atcgccccag gtcagctgct
    13321 tgctggcgtt gaccataacg cgatcggcga taaaaatggt cgctcctgcc acaagtaaca
    13381 gtaagcaggc aatctttaga gtccggcgag aaaaacaaat acggagaaga aggcgggcaa
    13441 atgcgcgcag catggtcgtt atccttaacg aaagggggga ggtcgatttt aggcgctaaa
    13501 gaaagaaaag ataacgcctg acatcatttt cagattttgt cctgcccaag cgacaactgc
    13561 gttagccaca gccgggtgtc gaactcaagc tggtggtact gcggctccat atggcagcac
    13621 aattgataga acgctttgtt atgctctttc tccttcaggt gcgccagctc gtgcaccacg
    13681 atcatgcgca aaaacggttc cggcgcgttg cgaaacacgg tagcgacgcg gatctctgct
    13741 tttgctttca gcttgctgcc ctgcacacga gaaacagcgg tatgtaaccc gagcgcgttt
    13801 tttagcacat ggatcttatt gtcatacatc actttattga tcggcggggc attacgcaga
    13861 aactgatttt tcagatcctg agtatattgc cagagagctt tatcggtggc gtagtcgtgt
    13921 gttcccggat agcgttttgc cagcacatcg cccagacgct gttcgttaat cagtgttcgc
    13981 acctgcgaaa gcagctgctc gggataaccc tgaagataag taagattgct cataaccgcc
    14041 cacgaaaaag agaaaaaggg tatactcacg cacccttttc aggggcaacg tcgaaatttt
    14101 atcattcagg agggccgatg agccacttag acaacggttt ccgttcactg acactacaac
    14161 gttttccggc gacggatgac gttaacccgc tacaggcgtg ggaagcggca gatgaatatt
    14221 tgctgcaaca gttggacgac acagaaatcc tcggcccggt gttgatcctg aatgatgcct
    14281 ttggtgcgtt aagctgcgcg ctggcggaac ataagccgta cagcattggc gactcataca
    14341 tcagtgaact ggcgacgcgc gagaatttac gcctaaacgg gattgatgag tcgagcgtga
    14401 agtttctcga cagcaccgcc gactacccgc aacagccggg tgtggtgctg atcaaagtgc
    14461 cgaaaacact ggcattgctg gaacagcaac tgcgtgcgct gcgcaaagtg gtcacgtcgg
    14521 atacacgtat tattgccggt gccaaagccc gtgacattca cacttccacg ctggaactgt
    14581 tcgaaaaagt gctcggcccg accaccacca cgctggcatg gaaaaaagcg cgcctgatta
    14641 attgcacgtt caatgaaccg ccgctggccg atgcgccgca gaccgttagc tggaagctgg
    14701 aaggtactga ctggactatc cataaccatg cgaatgtctt ctcccgcacc gggcttgata
    14761 tcggcgcgcg cttctttatg caacatctgc cagagaatct cgaaggggag attgtcgatc
    14821 tcggttgcgg taatggcgtt attggtctga cgctgcttga taaaaacccg caggcgaaag
    14881 tggtgtttgt cgatgaatcg ccgatggcgg ttgcttccag ccgtttgaac gttgaaacca
    14941 acatgccaga ggcgttggat cgcagcgagt ttatgatcaa caacgcgctc tccggcgtgg
    15001 agcctttccg ctttaatgct gtgctctgca accctccgtt tcaccaacaa catgcgctga
    15061 ccgataacgt cgcctgggag atgttccact acgcccgccg ctgcctgaaa atcaacggcg
    15121 agctgtatat cgttgccaac cgccatctgg attacttcca taaactgaag aaaattttcg
    15181 gcaactgcac cactattgcg acgaataata aatttgtggt gctgaaagcg gtgaagctgg
    15241 gacgtcgtcg gtaagattgg gagttacctg ccggatgcgg cgtaaacgcc ttatccggcc
    15301 tacgttcagc acgttacccc caggccggat aagacgcggc aagcgtcgca tccggcaatg
    15361 tctgctcagt tctggaatcg cgcttccata aaacaaaaaa tccctgtcac catcctctac
    15421 attctcttgt ttagcgtttt tctacgttta ttcttccgtc acacagataa attcatccgt
    15481 tgccaatctg tcaacggatg ttatcatatt gccaatttgt caacggacgt gatgaagaat
    15541 gcacctgata actcaaaaag cattgaaaga tgctgcggaa aaatacccgc aacataaaac
    15601 ggagttggtg gctctgggga acacgattgc taagggatat ttcaaaaaac ctgagtcatt
    15661 aaaagcagta ttcccatctc tggataactt caaatatctg gataagcatt atgttttcaa
    15721 tgttgggggc aatgaattac gtgttgtagc aatggtcttt tttgaatcgc aaaagtgcta
    15781 catacgtgaa gttatgacgc ataaagaata cgatttcttt accgctgttc atcgtactaa
    15841 ggggaaaaaa tgattgcgat tgccgacatc ttgcaagcag gagaaaagct aactgctgtg
    15901 gcaccttttc tggcgggtat tcagaacgag gaacaataca cccaggcgct ggaactggta
    15961 gatcatctgc tgctcaacga tcctgaaaac cccttgctgg atctggtgtg tgccaaaata
    16021 accgcgtggg aagaatcagc gcccgaattt gcggaattta atgccatggc tcaagccatg
    16081 cctggcggta tagccgtgat tcgtaccctt atggatcaat atggtttaac cctttccgat
    16141 ctgccggaaa ttggcagtaa atctatggtg tcacgcgttt tgagcgggaa gaggaaatta
    16201 acgctggaac acgctaaaaa attggcaacg cgattcggca tttctcccgc cttgtttatt
    16261 gattaacgcg acgtgccgaa tgcgttgcat ccggcgcttc aaataacgat ttaaatctcc
    16321 agcgccagcc gtgttccctg ggcaattgcc cgtcgtgcgt ccagctccat agccacatcg
    16381 cagccgccaa ttaaatgcac agttttcccg ctatcaatca gcggttgcgc cagcgcgcgg
    16441 tttggctctt gccctgcgca gatcaccaca ttgtccactg ctaatacctg cgtttcgccg
    16501 ttgatcacca catgcagccc gtcatcgtca atcttctgat aacttacgcc tgggatcatt
    16561 ttcacacccc gcgagagcag ggtggtgcga tggatccagc cggtggtttt gcctaacccc
    16621 tgtcccggtt tgctggcttt gcgctggagc atcacaattt gccgtgggct acgcgggatc
    16681 tgcattccct gcgggcttaa gccaccagcc tgttgtaggc tgctgtcgat cccccactca
    16741 ttacagaacc tggcgatatt ctggctggtg gattcgcccg gctgacttaa atacatcgcc
    16801 gtatcaaaac caatcccgcc acaaccgatg atggcaacct tgttgcctac cggcgctttg
    16861 tcgcgcagta catcgagata actcaatacc ttcggatgat cgatcccgtc gataggcgga
    16921 acacgcggca cgatcccact ggcgaggatc gtttcatcga acgcctgtaa ctgatccgcc
    16981 gtcacggtgt gattgagttt cagcgtcacg cccgtcactt cgatcatccg gcggtagtag
    17041 cgcagcgttt cgtaaaactc ctctttgccg gggatctgtt tggcgatatt aaactgcccg
    17101 ccgatctcgc tgtgcgcatc aaacaatgtt acctgatgcc cacgcgccgc cgcgttaatg
    17161 gcaaacgcca gcccagcagg tccagcgccg accaccgcca gatttttttt ctgcacggcg
    17221 ggaaggattg gcattttggt ttcgtggcag gcgcgaggat tcaccaggca cgaggtgact
    17281 ttgccaacga agatctgatc gaggcagccc tggttgcagc caatacaagt gttgatctca
    17341 tcggctcgcc ctgattgcgc ttttgacagc agttccgcat ccgcgagaaa cggtcgcgcc
    17401 atcgatacta tatcggcatc gccgcgcgag agaatatcgt cggcaacctg cggatcgtta
    17461 atccggttgg tggttaccag cggcagcgag acgtggcctt tcagtttgcg cgtgacccag
    17521 ctaaatgcgc cgcgcggcac gggcgtggcg atggtcggaa tccgcgcttc gtgccagcct
    17581 attccggtgt tgataatggt cgcgcccgcc gcttcaatgg cctgcgccag ttctaccgtt
    17641 tcggcaaaag tgccgccgcc ttcgactagg tcgagcatcg acaaccggta aataataatg
    17701 aagtcgttgc cgacgcgttc gcgcaccgta cgcactactt ctacggcaaa tcgcatccgg
    17761 ttgcggtaat cgccgcccca ctggtcacta cgctgattgg tgcgcagcgt cagaaattcg
    17821 ttgatcaaat acccttcaga gcccatcact tcgacgccat cgtatccggc ctcccgcgcc
    17881 agttgcgcgc agcgggcgaa atcgtcgatc agttgcagga tctcttcatg ggtgagttcg
    17941 tgaggaacga aacggttgat gggggcctgc aatgcggacg gtgcgaccag atgcggttgg
    18001 tagctgtagc gcccggtatg caaaatttgc agggcgattt tgccgccttc ctgatgtacg
    18061 gcttcggtaa tggtgcgatg gtgtgggatc tggctggcgt cgttgagcat tgcgccacct
    18121 tccatgccaa cgcctgttaa atctggtgca atgccgccgc tgacaatcag cgccacgccg
    18181 tgacgggcgc gttcggcata aaacgcagcc agccgttcag caccgtccgg gtattcctcc
    18241 agcccggtgt gcattgaacc catcaacacg cggtttttta acgtggtaaa acctaaatcc
    18301 agcggggcga acagcgacgg gtagctcata aaattgtcca gtatgtaaaa taattgttat
    18361 gtagtcggat gagttctaat ttagccgtcg atggagaaaa gggaaaaggg gagtgcgggg
    18421 tttgtgatgg gattcaaaaa gaatacgccc ggtggtaacg ccggatgcga cgcttgccgt
    18481 gttttatccg gcttggggga ttgcacatgt agggcggata aggcgttcac gccgcatccg
    18541 ccagccgttg caaattactg cttacggaaa aaatcgttat acaacatata caaatgagcg
    18601 gcactccagg agaaatttgg tgcgccttgc tgcgcgcctg tcagcgggtt gtaattctcc
    18661 tgaattgggc catcggcggt taatcctttg gcgtgcctga agaacgtatc cgccagcttc
    18721 agggcatcat cacgataacc gtaacgctcc atccctttca gaccaaacca gaactgatcc
    18781 acccatacgc gcccgcgcca gtagatatca gcgccaaaag ccggattggt taacgccgcc
    18841 gttcccagcg gaacaaatgt gttgaactct ttaggatcga gcatcacctt caccaccgcg
    18901 tcggcattgg cctgcgttgc cgcaccgtta aacagcggcg accagccttc cggcccttta
    18961 ccgcgctcaa caatcggttt gcccgcgcag ccgttcgcca gtggtttatc ttcaatacgt
    19021 acgtcatagt agaactgggt agtcgggtcg aacatacagg tgttgatgta gtccgcgagc
    19081 tgctgtgcca actggcgata gcgtttggcc tcttctggct taccgaggat ggttgccatc
    19141 tccgccagat aatggttatc gctgtacata tagctggcct gatccaccga ctcctgcaat
    19201 agcgagtagc ccagcaacgt tccgtcctga ctgcggtttt cggcgaattt caccgtccag
    19261 tcgctacgtt tgccgccatt agcgacatat ttatccaact gttctttgtc gataaaacca
    19321 aagacggcgg catcatcacg ccccgattcc cacgacgcag cgacctgtgc cggaatttcc
    19381 agactgtcgt actggccttt ctccaccacg cgggcgtagt tgttcagccc agactgcgtc
    19441 tcttctttat cgcctttttt caccgtaaac agcatctcgc cgctctcggt gttgtgggct
    19501 ttgtcgcggg tcgcgccata ttccggcacc ccgttgccgt tatgatcgcg gttacgtaac
    19561 caccagtcgt gataggccac cagtttcggg tacatctctg ccaaccaggc tttatcctgg
    19621 gtgacgttgt atacttccat caccgaccag gcggcaaggc tgggtttggt attacgttcg
    19681 ttccagttgc cgccatcgcc accacgctcg gggctaaggt tccacgctat caggtcgggg
    19741 acaaagccca catcctgcgg acgcacgctg tcgccaggct ggatctgcca ggagaagacc
    19801 gcgcggatat tctctttggc gatatccgga ttgaaatgcg ccatcgcaaa cgcctgcttc
    19861 caggtatccc acggccaggt ctgattgccg gagaaccagc gtccggtcac cgacggcgtg
    19921 acggtgttat aattcaccgc accgcccggt gagcgccagt taccgttgag cgtttcgatc
    19981 gctttcaccg cgacgcgcgt ctgttccggc gtcgcatccg gattggttaa ccctttcttc
    20041 aggtattctt cccagcgttg ctgcgaggcg gtgagataaa acgccggacg cgccagaata
    20101 tcgcggatct gcatttgctc tttgctaact tcctgagcgg tcagcagatg ggaataggtg
    20161 gtgtagagcg tggtcgaacc gttgatatgc gccttactgg taaagcgatt accgttgatt
    20221 tcagtctgca ccggcaggga tttatgcacc tgatattccg attcgccgga ggtcagcaga
    20281 tcccaggtgg cgcgcacttt gccaaaggtg actttcaggc catcacgggt ggcgctgatt
    20341 ttgcgctgat agtcagggta ttcgccagca atggttttat cggaaagcgg tttcccttct
    20401 ttcgcttcca gtttttccag cagttcgcca tcccacacca gatccagcgg tttattgctg
    20461 atgattttag tttccagtag tgacgtgcgc ggcgtggcga agcgcagagt catttcgacc
    20521 tgcacatctt ttgctgtcag tttttgcacc agcgcgccgg gaatactgta tgcctccagc
    20581 gtgaagttga ctttcttgcc gtcttgccag acagtcaggc ggtcgaaatt gctggccata
    20641 aagttgatgt attcttccgt cagcagcgca acgcccggaa agccgcccat ggtgttcggg
    20701 ccgtctggca acagatgacc atgccaggct ccgagatcga aaaacggatt aaaacgctgg
    20761 tgatcgtcgt aatcgtaatc cttcatgtac tgcggcgcac cagtacggtt aatcacgttt
    20821 ttataattgt cggcgttagc ggcatgagcg gaaaaactta tcagcagagc gcaggttact
    20881 ggcgttaaaa tagttttgat tttcatgaga ttacagtcct tattaccagt agaaatattg
    20941 catcaggaag atttggtcgc tgggatcgtt agaggcattt ttcatgtaga agcggctatc
    21001 aaaagccgtg gcgaaacgtc cgccgccata ttcgtaccag ataccaaact gcatgaaaga
    21061 ggtggtttca tcgtcagcgt ggtcagggcg atgtttggcg taggtgtaag cggtggagag
    21121 ataaacgcct tttaacgctt catacatggc gctcaccatc atcccgcttt cacttcccgg
    21181 tacgtccagc ccgtcggcac gtccggtatg gtgccaggcg cgagcggcaa agttaggggt
    21241 aaacaggccc gtgagataga gttgtcctgt gccgtcggta atttccagac cattcatcca
    21301 ggtgatgcct tcggcaaggt catactggat gtaaccgttc atccattgcc ggataggtgt
    21361 atttgtcgtc gtagcgatcg tacttgccaa aatgtagcca ggctttgctt tcgtcatgat
    21421 gtccggcagg tgtggcggtg acgctataac gaaagttgcc gcttaagttc tgtaccttca
    21481 gcgcggtcat aatgtcgcgg gtattgggaa tgacgtagcc caaatcgggt gtgaagtcgc
    21541 cccaccattg caggtcatcc agcgaggaat cctgacgcag actgagcatg atttcggtgc
    21601 catcattggt acgataaccg ccatagaaac ggttaatccc accttcgaaa ccaccccagc
    21661 tatggtcagg cacctaggca ttaccgtcgt ggtcggcctg cacggtccag ccttcgccgt
    21721 aaatcaggcc gaaccaggag ccgtacttca tctccaggcc accttcaata taggtgccgt
    21781 cgctgtattt atgtttattc tcaccctcta aatccatata tccaccaagc cctaattcgc
    21841 cgtaaaaacg aaatgaagga gaagatgtcg aggtaattgc gggtgttttt gtgtcgtcat
    21901 gttctgccgg acgtgcttct tctcctgccg cagaaaaggc gtagcaaaac ggcagagcaa
    21961 gtaatgctct gcatggtgca gtgattaatt tcattgtgtt aatccatgag ctaaagaaaa
    22021 agtacagcct tttatcgttg taattataaa aatataaagg cagaaactat tttccctctc
    22081 ctcatccagg aagagggaaa atgcctgctc attttttatt agtctgctta atcaacattg
    22141 ctggctggcg tcgcttcaat atggcgctct tcagccgtta atccctttat atatttgctg
    22201 tatttccacc acgcaaagcc gaggaagata acaataccgc cgacgttata aaaaatgatg
    22261 gtcagaatat tcgcgccagt cgggaatgtc gaagcgacaa accctacggc aaatatcgca
    22321 atcagcattg aaacaacgat aataccggtg cggcgggagc ccatacggaa atcgcgtggc
    22381 aggtgatcta atttggcgcg taaattcagg taagccagca tgatgaataa cggcggaagc
    22441 atggacgctg cggcggtcat attaataatg gtattcatca gatcctgcac ggtattggaa
    22501 ccgagcatcg ggataatcat cagcgggatg acgatcagaa actggatcca tgctgcgcgc
    22561 gccggaacgc cgttttcgtt aagttcgacg gttttcttac caaagatgcc ttccgggatt
    22621 tcggagaaga aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacattgcg
    22681 gtaaaggaca ccagaccgac aaagcgattc atcaacgctt ccggcagacc aaaatacgcc
    22741 gccatgccgt ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg
    22801 aagacgttaa tcagcaccga ggagacggaa tacagtacgc cgataaaaat cccggcgagg
    22861 atgatcactt taacgaacga tttcgaaccg cctttgacgt cgttaacgta caccgcgacg
    22921 gactccgcgc cacctgcggc cataaagatc caggtggtaa cgccgaggaa cgcccagttg
    22981 aagttcggga tcatcgcgtc aacggtgatc ggatcggcag gctgtacgcc gccaaccagt
    23041 gccgtacctg ccagtaaaat gtaggagagc gttaacagca gcatcagcgt tgaagtgacg
    23101 gaggtaattg gccccagcat tttcgccccg ttggtggaaa cccaggtgga gaaggcgaac
    23161 agcaccatac tgataacggt ggtggcaacc ggcgtcataa tatattcgta gccgaggaag
    23221 gcatacgaag cataagcaat aacgcgcggc aacagcgagg tgaaaaagaa cagatttacg
    23281 aaccagtaag tgtaggcagt aataaatgcc caacgtccgc ccagcgaact ttttacccac
    23341 gcgtagacac cggcttctga gtttttattt aacgaaacaa attctgcgat gatcagacag
    23401 aagggaataa aataaaaaat cgtcgcgagg aaaaacatcg gtgccgaggc cagtccaagc
    23461 tcaatattat tattgataac gttgttaaag ctgtaaacgg cggcaaaagt cagcgagagc
    23521 aagccgaatt tgccgattgt attacgtttg gtatcagaca taacacatct ccgaaaatgt
    23581 gtagggtagc ggtggcggca aactcctttt gccgccgtag ttgttatttg ttatggaaat
    23641 aaccatcttc gatggtgact ttgagaacca cttttttgac cgcgttattg cagataaacc
    23701 gatacgcttc atggatatcg caaataacga tttgcccttc gtggacctct acggtttctc
    23761 cgcagccttt taaatattca cggtcagttt catcacgata atattccact acctgtaatg
    23821 tctctttcgg cgcatattca attttttgct gcccttgcag gtagtaatgc acttcaaaat
    23881 agcgtcgatg cccggtaaat agcgcatcgg tcgcggagtc attttccaca cggtaggtca
    23941 atgaatcacc gatggagtgg gcgacgccag gctgaatgtt gtcgatattt tcaatcgctt
    24001 caacgcagcg ttgccacttc ttgccagagg cgtaaatctg gcggaactgt tctaagttat
    24061 cgatgatcct cattgcttat tctcgctgtg caaattcgtg gaaaagaacc ctgcgccgaa
    24121 ctcatacgac gccaggcttt gcgcggtagc ttctccgcca gaaaccggca gcaacgtaaa
    24181 gccgtagctg aagtcacgga accagacgcg ccaggagtcc agcacctcgc tgccccagga
    24241 gttggagccg aggccaagta gctggtggtc gaggttcagg gtgatgtcat cactgcgctg
    24301 cagctcgtta cagtgctggg cagcgtggat gttttcctgg gtatagtgcc aggcgctgaa
    24361 gttaattggg cgctgcggaa ccaccagcag accgttgccg tggcggttag tcagtgccgt
    24421 ccagcggaca tgctgacggt tgccgttgtt ctgcgggaag ggatagttct cgaacatggc
    24481 atcgacggtg ctgcgccaga tatcgatgat gttagcctgc tggctgtcgg cgtagttttc
    24541 gcccggtcca cgaccgtaat acgccacctg atcgtattcg ccgttaatcc ccatggtgaa
    24601 accgatgcac ggaatgatgt gcggatagtc gccgtaacgc tcgccggaaa gcgccacgtt
    24661 aacctggcca tcggcagcga tgcgccagat gtaggtacag cgcatcccga agtcaaacac
    24721 cggcggtgca ataactgtgc ggctgatgat caacacttca ccatcgctct gttctacggc
    24781 aaagtcgcgc agatgttcct gcatgatctg caaatgattc ggctgccaca acccttcgta
    24841 ctcctgcttg tggttgtcga tcatcggctt gaagaagttg atctttggct cgcgggtcag
    24901 cagcgactcg ccatttacct gccaggatgt cggtttgcca ctcatttttg agaaggtgat
    24961 cgcgaagttg tagccgcgaa cggtgcagct caaacgatcg tcttccagcg tcagcggacg
    25021 cgcattattt ggtgcgaaag gcactggctg cgcggtgttt tccttcagcg ggaactgata
    25081 agtggcgata gaatgcccgg cttcgctgta gcgggtgcgg gaatctttgg tcaccgtaat
    25141 gttgaggaac gcttcgcggg cgtccagctg cggcagcgtg atctgcaagg gggcttcgct
    25201 gttcggcgca acgtcgcgca gtttaatctg ctgcgtcgcg agcgtttcac cttcggcgcg
    25261 cacctctgcg tgcagggtgt agtcatcaag cgtggtaaac cacagtttat tttcgacttt
    25321 caactcgccg cgagtcagcg cgtggatttt taccggcgcg ataacctgtt tgtactcttt
    25381 caggcccgat cccggcgtct gatcggaata gatcaaacca tcaagacaga agttatagtt
    25441 gttgggatag tcgccgtagt cgccgccgaa tttataccag acattgccgt ggtcgtcctg
    25501 tgcctggatc ccgtggtcgc accactccca gacataatga ccctgaatgc aatcgtgctt
    25561 atagaagacg ttctggtact ccgtcagccc gcccggtccg ttccccatcg catgagcata
    25621 ttcacagatg atgcgcggct tcggatgcgg gtattcacca aactcattca tcagcggcac
    25681 gcgggtgtac atggtggaaa taatatcgac cacttcagca tcgcgatctt cttcgtaatg
    25741 aaccagtcgc gtgtcatcca gcgctttcgc cgcatggtac atcgcgcgga tgttacagcc
    25801 atagccggat tcattgccca gcgaccagat gatgatcgac ggatggtttt tctgtgcgtg
    25861 gatatggcga acaatgcgct cgacgtagac tttttcccac tgcggatcgt cggtaatacg
    25921 gctgatatcg ccgacattag caaagccgtg cgattcgacg tcggtttccg ccatcacaaa
    25981 caggccgtag atatcacaca gttcgtaaaa acgcggatcg ttcgggtagt gagcggtacg
    26041 cacggagttg atattgtgct gcttcatcaa ctggagatct ttctcgacgc gatccattcc
    26101 aacggcgcgg cctttgcgat gatcgttgtc gtgacggttg acgccgtgca gcatcacata
    26161 acggttattg atccagaaca gaccgtcgcg cactttgata tcacggaagc caacgcgttg
    26221 tggcaccact tccagaacgt tgccgttggc gtctttcagc gtcatgacca gatggtaaag
    26281 ataaggggat tctgctgacc attgctgcgg ctgttcgaca gtaaaagcaa agctggcgct
    26341 ggtcagtttt tcaattgcca aatgatcaat ggcgctgctg tgcaccacgc gttcgccatc
    26401 aaacagggta tattccagcg tcgtgacgac aggggaggcg gcgagatttt ccagcaccac
    26461 ttcgcaggaa agcgtggcat cgcaataggc ttcgtcaaag tcggtacgca cagtgaaatc
    26521 gttaatatgc gttaggtgtt ttccgaccag ataaacatcg cggaagatcc ccgccgacca
    26581 ccacatatcc tggtcttcca cgtaggtaga gtccgcccac tgcatcacgc gcacacacaa
    26641 caggttgtcg ccggttttaa ccatcgcgct gatgtcaaac tctgcggtca ggcgactgcc
    26701 cttgctgaaa cccacatact gaccgttaac gtagacttca aaataggttt cgacgccgtc
    26761 aaatttaatc agcgtctgtt taccctgcca gccgtcgctg agggtgaaaa tacgttgata
    26821 ggcaccggtt gggttatcgc tggggacaaa cggcacatcg atggggaacg gaaaaccttc
    26881 gtcggtatat tgcagtttgc cgtgaccttc catttgccac atggcgggga cggtaatatg
    26941 cccccagtca gccattaact cagaggtgaa ggcttctggt acttgcagcg gatggtcaaa
    27001 aaagtggaaa ttccactgac tgcttaaggg cagaaacagg ctgctggttt cgcgggcaaa
    27061 ggtacgcgct tgcgcaacag aatcatatga aaaaaagtac gcacgcggcg caagtcggtt
    27121 ttcgtgggtg agctgaatgt tttcccagcg attcataggg cctcccggta gagagaaatc
    27181 ggaaccattc atggtgatgg tagtttgtgt aattgctggg ggtttagtaa aatttttact
    27241 aaaaagcagc aggtaaatgc atttacttta actggatcac gaaaataaac ggcagcactg
    27301 tccggcaaaa atgattgccg aacagtgtgt aaggggattt aacgggtcgt gccgcgcagt
    27361 tttaatttgc tgggaacgaa gactagcagc ggcagcgcgc gaccatcgcg ggctttttca
    27421 tacaccaggt taacgccctg acttcccatc atctcggaat ggatgcgcac ggtggagagc
    27481 ggcggaaagg taaatcgcgc ggtggggata tcgttaacgc tgataagcga aatatcttgt
    27541 gggatgttca ggccacgttc atgaattgcc cgcagtacgc cgatagcaat ggaatcggaa
    27601 gcaacaaaca gtgccttcgg atagtcttcc cgcgccagca tttgttttgc cagttcataa
    27661 cccgacgaac tggaaaaacc gccgcgccag atatcctctt cgcgtaccac ttgtttcagt
    27721 cggccatatt ccgcaaaggc gacctcacga atatccgcct tgccaggctc atcttcaccg
    27781 ccaataaaac caatacgatt aacgccctgg ttgatataga agtcgatgat ttctttactg
    27841 atgcgtgcca gatcgatatc caccgcatcg taaccgctgc cgggttcgtg aaagtcgata
    27901 aaacagatat tgtcggtcaa cgcgctggca gcggcgcgca gggcgggcgt gggtttgccg
    27961 acaattaaaa taccggtgac gtttttaatg tctggtaatc cgttgtgttc ataacagttg
    28021 gtcagttcga tcgccagctt ttcgcactga gtttcaatgc cgtggcggat cgccagatag
    28081 taaggatcgt tgatctccag ctcctgctgg tagctgtaga tagccagaat atggtgttgg
    28141 ttgactgcgc ctgtctgtag tttacgggcg ctactggtct tgtactccag cttttcggcg
    28201 atctcgagaa tgcgatgttt cgtctcttct ttcacattca atgtcggatc gtcatttaag
    28261 accctggata ctgtcgccag ggatacgcca gcttcgattg cgatgtcttt taatgttgcc
    28321 atgtttacct tttttacttt ccctgatgtg cgcccccatt gtagaggaag tgcttatcgg
    28381 gtcacctgtt ttcagtaaat aaagcgcgtt tctttgacgc tgtaacggta acacagcggc
    28441 ttaggaatgt gatcacgcct gcattctcca tttttagcct acaggattga gaaaaacttt
    28501 tctatagttt agtaaaattt tactcgaagg gaggaagcaa tggaaaccgt ggcttacgct
    28561 gattttgcac gtctggaaat gcgcgtcgga aagattgtgg aagtgaaacg ccatgaaaac
    28621 gccgacaagc tgtacatcgt acaggttgat gtggggaaaa aacactgcaa accgtgacca
    28681 gcctggtgcc gtactacagc gaagaggagc tgatggggaa aacggtggtg gtcttatgca
    28741 atctgcaaaa agcgaaaatg cgtggtgaaa cgtcggaatg catgctgttg tgtgcggaaa
    28801 ccgatgacgg cagtgaaagc gtgttgttaa cgccggagcg gatgatgcct gcgggcgtac
    28861 gcgtcgtata aaaagatcgg atggcgacgt cgtatcgcca tccgatttga tattacgctt
    28921 cttcgacact tactcgcatg gccgccagcg ccttacgcgc cgctttgatc accagttcac
    28981 actgttcaat ggtcagtgtc agtggcggtt caatgcggat cgttttggcg ttattgagcg
    29041 ttccggccac cagtacgcgc tggcggaaca tctcgctggc aaagttatag ccgatttcgt
    29101 tatcaacaaa ctcaatcgcc atcaacatcc ctttaccacg cgcttcctgt accagatcgg
    29161 gatattcccg cgccagttga cggaaaccgt ccagcaacat atcgcctttt tgctcagcct
    29221 gagccggtaa gttctgctcc agcaacacat tgatggtcgc cagcgccgcc gcacaggcca
    29281 gcgggttgcc gccaaaggtg gtggtatgca ggaatgggtt gtcgaacaga actgaaaaca
    29341 cctcttcagt ggcgatggtc gcgccaatcg gcatcacgcc gccgccgagg gcttttgcca
    29401 gacacagaat gtccggctga acgttctcat gttcgcaggc aaacatcttg cccgtgcgcc
    29461 ccatgcccgt ttgtacttca tcgaggatca tcagtgcgcc gaactcatcg cacagcttac
    29521 gcacggcggt gagatagccc ggcggcggca gaattacgcc accttcgccc tgaatcggtt
    29581 cgaggatcac cgcagccaca tcatcaccgg tttttttgca ctcattaaga gccgtgcgca
    29641 tggcttcgat attgccaaac ggcacatgac ggaaccctgg cagcagtggc ataaacggtt
    29701 tgcggaaggt cgatttcgcc gtggccgaca gcgcgccaag tgatttaccg tggaacacgc
    29761 cgctggtggc aataaaagta aatttgccgc gcggcgactg ataagccttc gccagcttca
    29821 gcgccgcttc aacggactcg gtgccgctat tacagaagaa gctgtatttc agtttaccgg
    29881 gcgttagcgc agcaagggtt ttcgccagca tagcccgtaa cggatcgagt agctcctggc
    29941 tgtgcagtgg ttgtttcgca agttgattct gtacggcgga aaccacaact ggattacggt
    30001 gccccacgtt gaaaattcca aaacctccca ggcagtcgat aaactcctgt ccctgggtgt
    30061 cgacaagcgt atttaaacct cccgcttgcc actctacggc tccgtaatcc ccgccggcgg
    30121 taacagattt gcgatactct aaaaaccccg gattgacatg ctctttgaag tattcaatca
    30181 cctctcggtt aagtgctttc atctcctcat gatccagcgt tcgcttctca atgagattca
    30241 gggcgtgggc gctgcacgct aaagccgatg cgctcgaagg taacctgttc aaaatatgct
    30301 ccggaggctc gcgtatcaca tgataccgat ttaagtattg cagggattgc gccactccgg
    30361 ctgcgatcgc gaaaatcggg ataaacacaa atgcggttat catttgtgca atatttaatg
    30421 ctatccgtta acatttgttg ttattttgca cggtttttgt cgggtatgga tttgatgatt
    30481 gctgcgcaaa tgcacgtttt cagggcaata attgcctgat ttttgggcaa gataattaac
    30541 gttttcgtat agtaacagag tgaaagtatt ttaaatcaaa gaattagatt tgtgctttaa
    30601 tcgcaaattg cgatctaaat caaattaatc ggttaaagat aaccgcagcg gggccgacat
    30661 aaactctgac aagaagttaa caaccatata acctgcacag gacgcgaaca tgtcttctca
    30721 tccgtatgtc acccagcaaa ataccccgct ggcggacgat accactctga tgtccactac
    30781 cgatctgcaa agctatatca ctcatgctaa tgacactttt gtgcaggtga gcggctttac
    30841 cttgcaagag ttacaagggc agccgcacaa catggtgcgt cacccggata tgccaaaagc
    30901 ggcgtttgcg gatatgtggt tcaccctgaa aaaaggggag ccctggagcg gcatcgtgaa
    30961 aaatcgccgc aaaaatggcg accattattg ggtgcgggcc aatgcggtac cgatggtgcg
    31021 cgagggaaaa atcagtggct atatgtcgat tcgtacccgg gcgacggatg aagagattgc
    31081 ggcggtggag ccgctgtaca aagcgttgaa ctccggacgt accagtaagc gtattcataa
    31141 aggcctggtg gtgcgtaaag gctggctggg taaactgcct tcattaccgc ttcgctggcg
    31201 gacgcgtgga gtgatgaccc tgatgtttat cttgctggcg gccatgcttt ggtttgttgc
    31261 tgccccggtg gtgacgtatt tcctctgtgt gttagtggta ttgttggcaa gcgcttgttt
    31321 tgaatggcag attgtgcgcc cgatagaaaa tgtcgcccgt caggcactga aggtggcgac
    31381 cggagagcgt aatagtgttg agcacctgaa tcgcagcgat gagctggggc tgacattacg
    31441 cgcggtaggg cagcttggcc tgatgtgccg ttggctaatt aacgatgtct caagccaggt
    31501 gtccagtgtc agaaatggca gtgagacgct ggcgaaaggc accgatgaac tgaacgaaca
    31561 tacccagcag acagttgata acgttcagca aacggtggcg accatgaacc aaatggcggc
    31621 gtcggtgaaa cagaactctg ccacggcgtc ggctgccgat aaactttcta tcaccgccag
    31681 taatgcggca gtgcagggtg gggaggcgat gaccacggtg atcaagacaa tggacgatat
    31741 cgccgacagt acctagcgca ttggcaccat tacttcgctg attaacgata ttgcgtttca
    31801 gaccaatatt ctggccctga atgcggcggt ggaagcggcg cgtgccggcg aacagggcaa
    31861 aggttttgca gtggtggcag gggaagtgcg tcatttagcc agccgcagcg ctaatgctgc
    31921 caacgatatt cgcaagctga ttgatgccag tgctgataag gtgcaatccg gttcgcagca
    31981 ggtacacgcc gccggacgga cgatggaaga tattgtggca caggtgaaaa acgtcaccca
    32041 gttgatcgcc cagattagcc attcaacgct ggaacaggcc gatgggcttt ccagcctgac
    32101 ccgtgcagtg gatgagctta acctgatcac ccagaaaaat gccgagctgg tggaagagag
    32161 tgcgcaggtg tcggcgatgg tgaaacaccg cgccagccga ctggaagacg cggtgacggt
    32221 gctgcattaa tcggtatgcc ggatccggcg gttggagcac aatgcctgat gcgatgctgg
    32281 cgcatcttat caggcctgcg gggtgtgtag cgggtgtagg ccggataagg cgcgtcaagc
    32341 gtcgcatccg gcaattgcac cgcgccactg gcggatgcgg cgattaatcc agttgcgtaa
    32401 tatcaaaagc ggcgcggtcg atcaccgcaa tgatcttttt tatttgtgca tcactgatat
    32461 cgctctggtt gacgcgtaaa tccagcaccg ctttaaaatt atccagcgcc cgcttcattt
    32521 gcgggttctg gcgcagcgcc gcgccaacgc aacgcgcttt gatgcgttct tcaatcatct
    32581 ccacttgttc gcggttttct tccagccact gcgcgccttg ttcggtcagc gcaatctgct
    32641 tcttaccacc ttcctcttcg cggatggtaa tcagcgactg ctcctgcaga aaatccagcg
    32701 tcgggtagat gacgcccggg cttggggtgt aattcccctg ggttagattc tcaatcgctt
    32761 taatcaattc gtaaccgtgg ctgtcatcgc gcgagagaat atccagaatc accagacgca
    32821 attcaccgtg accaaagaaa cgttgccgac gaccgccgcc gcgaccgtgg cgaccaccgc
    32881 aacattgacc gtgttcatgc tggtgaccgt gtccgcagtg ctcgtgttct gacttctcac
    32941 ctttgcagca gccctcatgg cgtggctggc cttcatgttt acaacaccct tcgtgatgat
    33001 ggctcatatc agcctccttt tgcttgatta gatatatcta atttatatct gatgtgagca
    33061 ttactgcaag tacaaaaatg atttttaaac gtggaagcac ctcacatcta ggccggataa
    33121 gacgttcacg ccgcatccgg cagcattgct atggcactga atgccttcac atacttcaga
    33181 catatcagtt ctacaaatcg cttgcattta tcatgattaa caatcattat catttgcgaa
    33241 ttttatttag atatatctga ttaccatcac gaaggcgata acaatgaata acaccccccg
    33301 ctacccgcag cgcgttcgca atgatctgcg cttccgtgaa ctgactgtgc tacgcgttga
    33361 gcgcatcagc gccggttttc agcgcattgt ccttggcggc gaggcgttgg acggttttac
    33421 atcgcgcggc tttgacgatc acagcaaact cttctttcct caacctgacg ctcactttgt
    33481 gccgccaacg gtaacggaag agagcatcgt ctggccggaa ggaccacgcc caccgtcgcg
    33541 tgactatacg ccactgtatg acgaactacg ccatgaactg gcgatcgatt tctttattca
    33601 cgacggtggg gtcgccagcg gctgggcgat aaacttacgg tggcaggtcc gcgcggttcg
    33661 ctggtggtgc cggaagatta cgcgtatcag ctgtatgtct gcgatgaatc cggaatgccc
    33721 gcactgcgcc gccgcctgga aacgttgagc aaacttgccg ttaaaccgca agttagtgcg
    33781 ctggttagcg tgcgggataa cgcctgtcag gattatctcg cgcaccttga tggttttaat
    33841 atcgaatggc tggcacatga tgagcaggcg gtagatgcgc gtctggcgca gatgcaaatc
    33901 cctgccgatg attacttcat ctggataacc ggcgaaggta aagtcgttaa gaatttaagc
    33961 cgccgctttg aagcggaaca gtatgaccca cagcgggtac gtgcagcggc ttactggcac
    34021 gcaaagtaac tgatatataa aaagaaaaag gctgacgatt tctcgtcagc ctttgctata
    34081 tctggtggcc cctgctggac ttgaaccagc gaccaagcga ttatgagtcg cctgctctaa
    34141 ccactgagct aaggggccat ggtagcggat tataaagtaa ctccgtgccg caatccagcc
    34201 attaccgcgc gcctgctgtt tttataaaca atgtattttc aatccgttat acttttcctg
    34261 gtgatgttag aaaggagtaa atatggttga ggatattttg gctccagggt tacgggtcgt
    34321 gttttgcggt atcaaccctg ggctttcatc cgccgggact ggttttccct ttgctcatcc
    34381 ggcaaatcgc ttctggaagg tgatatatca ggccgggttt accgaccgtc agttgaagcc
    34441 gcaggaggca cagcatctgc tggattatcg ttgtggcatc accaaactgg tagaccgtcc
    34501 aacggtgcaa gccaatgaag tttcaaagca ggagctgcac gcaggtgggc gtaagctgat
    34561 tgaaaaaatt gaagattatc agccgcaggc gttggctatt ctgggcaaac aagcatatga
    34621 acagggattc agctagcgcg gtgcacagtg ggggaaacaa acgctcacca ttggttcgac
    34681 gcagatttgg gtgctgccaa atcccagcgg tttaagtcgc gtttcactgg agaaactggt
    34741 tgaagcgtat cgcgagctgg accaggcgct ggtagtgcgt gggcgataaa aaacgtcacc
    34801 g
//
LOCUS       DAJTRD010000035        34761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTRD010000035 DAJTRD010000000
VERSION     DAJTRD010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.035"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..577)
                     /locus_tag="OO315_001877"
     CDS             complement(<1..577)
                     /locus_tag="OO315_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5890805.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFD"
     gene            669..755
                     /locus_tag="OO315_001878"
                     /pseudo
     CDS             669..755
                     /locus_tag="OO315_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1160..1636)
                     /locus_tag="OO315_001879"
     CDS             complement(1160..1636)
                     /locus_tag="OO315_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5890806.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1695..2984)
                     /gene="bioA"
                     /locus_tag="OO315_001880"
     CDS             complement(1695..2984)
                     /gene="bioA"
                     /locus_tag="OO315_001880"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5890807.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            3071..4111
                     /gene="bioB"
                     /locus_tag="OO315_001881"
     CDS             3071..4111
                     /gene="bioB"
                     /locus_tag="OO315_001881"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5890808.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            4108..5262
                     /gene="bioF"
                     /locus_tag="OO315_001882"
     CDS             4108..5262
                     /gene="bioF"
                     /locus_tag="OO315_001882"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5890809.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            5249..6004
                     /gene="bioC"
                     /locus_tag="OO315_001883"
     CDS             5249..6004
                     /gene="bioC"
                     /locus_tag="OO315_001883"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5890810.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            5997..6674
                     /gene="bioD"
                     /locus_tag="OO315_001884"
     CDS             5997..6674
                     /gene="bioD"
                     /locus_tag="OO315_001884"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5890811.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            7253..9274
                     /gene="uvrB"
                     /locus_tag="OO315_001885"
     CDS             7253..9274
                     /gene="uvrB"
                     /locus_tag="OO315_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5890812.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(9466..10374)
                     /gene="yvcK"
                     /locus_tag="OO315_001886"
     CDS             complement(9466..10374)
                     /gene="yvcK"
                     /locus_tag="OO315_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5890813.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10639..10783
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10771..11760
                     /gene="moaA"
                     /locus_tag="OO315_001887"
     CDS             10771..11760
                     /gene="moaA"
                     /locus_tag="OO315_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5890814.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11782..12294
                     /gene="moaB"
                     /locus_tag="OO315_001888"
     CDS             11782..12294
                     /gene="moaB"
                     /locus_tag="OO315_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5890815.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            12297..12782
                     /gene="moaC"
                     /locus_tag="OO315_001889"
     CDS             12297..12782
                     /gene="moaC"
                     /locus_tag="OO315_001889"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5890816.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12775..13020
                     /gene="moaD"
                     /locus_tag="OO315_001890"
     CDS             12775..13020
                     /gene="moaD"
                     /locus_tag="OO315_001890"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5890817.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            13022..13474
                     /gene="moaE"
                     /locus_tag="OO315_001891"
     CDS             13022..13474
                     /gene="moaE"
                     /locus_tag="OO315_001891"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5890818.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13611..14315
                     /locus_tag="OO315_001892"
     CDS             13611..14315
                     /locus_tag="OO315_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5890819.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            14520..15233
                     /locus_tag="OO315_001893"
     CDS             14520..15233
                     /locus_tag="OO315_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5890820.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15269..16225)
                     /locus_tag="OO315_001894"
     CDS             complement(15269..16225)
                     /locus_tag="OO315_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5890821.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16225..17466)
                     /gene="clsB"
                     /locus_tag="OO315_001895"
     CDS             complement(16225..17466)
                     /gene="clsB"
                     /locus_tag="OO315_001895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5890822.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(17463..18224)
                     /locus_tag="OO315_001896"
     CDS             complement(17463..18224)
                     /locus_tag="OO315_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5890823.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18357..18767
                     /locus_tag="OO315_001897"
     CDS             18357..18767
                     /locus_tag="OO315_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5890824.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18729..19835)
                     /locus_tag="OO315_001898"
     CDS             complement(18729..19835)
                     /locus_tag="OO315_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5890825.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19846..20979)
                     /locus_tag="OO315_001899"
     CDS             complement(19846..20979)
                     /locus_tag="OO315_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5890826.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20972..22708)
                     /locus_tag="OO315_001900"
     CDS             complement(20972..22708)
                     /locus_tag="OO315_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5890827.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22701..23696)
                     /gene="hlyD"
                     /locus_tag="OO315_001901"
     CDS             complement(22701..23696)
                     /gene="hlyD"
                     /locus_tag="OO315_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5890828.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23699..24370)
                     /gene="cecR"
                     /locus_tag="OO315_001902"
     CDS             complement(23699..24370)
                     /gene="cecR"
                     /locus_tag="OO315_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5890829.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24599..25786
                     /gene="rhlE"
                     /locus_tag="OO315_001903"
                     /pseudo
     CDS             24599..25786
                     /gene="rhlE"
                     /locus_tag="OO315_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(25993..26475)
                     /gene="ybiA"
                     /locus_tag="OO315_001904"
     CDS             complement(25993..26475)
                     /gene="ybiA"
                     /locus_tag="OO315_001904"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5890830.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26595..28745
                     /gene="dinG"
                     /locus_tag="OO315_001905"
     CDS             26595..28745
                     /gene="dinG"
                     /locus_tag="OO315_001905"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5890831.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28773..29735
                     /gene="ybiB"
                     /locus_tag="OO315_001906"
     CDS             28773..29735
                     /gene="ybiB"
                     /locus_tag="OO315_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5890832.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            29876..30961
                     /gene="hcxB"
                     /locus_tag="OO315_001907"
     CDS             29876..30961
                     /gene="hcxB"
                     /locus_tag="OO315_001907"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5890833.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(31190..31450)
                     /locus_tag="OO315_001908"
     CDS             complement(31190..31450)
                     /locus_tag="OO315_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5890834.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31715..31981)
                     /gene="ybiI"
                     /locus_tag="OO315_001909"
     CDS             complement(31715..31981)
                     /gene="ybiI"
                     /locus_tag="OO315_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5890835.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(32055..32732)
                     /gene="ybiX"
                     /locus_tag="OO315_001910"
     CDS             complement(32055..32732)
                     /gene="ybiX"
                     /locus_tag="OO315_001910"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5890836.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32774..34761)
                     /locus_tag="OO315_001911"
                     /pseudo
     CDS             complement(32774..34761)
                     /locus_tag="OO315_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8234 a         8938 c         9389 g         8200 t
ORIGIN      
        1 tatcaaaagc acttagttcc tgtagttcac atggtaattc tggtaaggca gtaagctggt
       61 tgttgccaac agaaagtttt tgtagagccg ggggaagatc aggtagcgtc tccaggctat
      121 tgagaagggc tgagagtgac tgcaaggaag atggaagaga aggcaaacat gttaaaactc
      181 tgttaagtga aacatcaaga gcgaccaggt gaggcggaag agccgggagc ctgctcagtc
      241 tgttatcgct ggctttaagt acagttaagg acggagggag ttctggcaga gagcgtagct
      301 cattgctaga gatgttaagt tcttgtatgt gcgggggcag gtatgggaga gagcgtaatc
      361 tgagtaaact taaattgagg gctggctctt gaaaagccag acatattttc agtaatcgaa
      421 ctgcctgtgt tcggtcttct gttgcagcac cttccttggc ccagttatcc cagatgcggt
      481 gataatccgt gatattttgc tcttctacgg ataaacggga tatacatcct aaaccacaaa
      541 aggagcggaa aaaagaagga ttgtgggcag gtttcattta ttattgatcc tcattgttta
      601 tatattaata tattcgtgca aatggagtga ggattcttta aaaaaacgcg cctgtaaata
      661 gttaagaaca ggaagaactg ttggggatgt ggctggccga aaatgaaggg gtgaagttct
      721 ggctcaatgt gctgactgaa ctgaaaaaac gcggttggtc gccctacctt ttcatcaagc
      781 cagtagtctg cccaccacag catcatttct ctgcgcttat cgagatactg agcatggttg
      841 taaattcagc taacccagtg cgttgacaaa aaattagcgc aagaggacaa aaaatcacct
      901 tgcgctaatg ctctgtatca ggtcactaat accatctaag tggttgattc atagtgactg
      961 gatatgttgt gttttgcagt attatgcagt ctatttttta agctaaatgt attataatgc
     1021 attgatatta atgattttta ctgtttctcg gtcagctttt ttatactaac ttgagcgaaa
     1081 cgggaaggta aaaagacaaa aagttgtttt taataccttt aagtgatacc agatggcatt
     1141 gcgccatctg gcagagtgat taactaaaca tcgcggtaat cgatgcgctt gccagagagt
     1201 ggaaatgaac gttaaacccg accatcgcgc cgctggctcc ttcatcgaca tcaatacgct
     1261 ctacatccag cgcgtgaacg gtaaaaatgt agcgatgggt ttcgcctttc ggcggcgctg
     1321 cgccatcgta tccggtttta ccaaaatcgg tacgcgtctg caaaacgccg tctggcattg
     1381 ctaccagacc agagccaaac ccttgcggta atacgcgggt atcagcgggt aaattaacaa
     1441 ctacccagtg ccaccagccg gagccggttg gcgcatccgg gtcgtagcag gtgacaacaa
     1501 aacttttcgt tcccgcagga acatcatccc acgccagatg cggtgaaata ttatcgccat
     1561 cgtaacccat gccgttaaag acatgacgat gcggcaactt atcgccatcg cgcagatcgt
     1621 tactgatgag tttcatgaac cctcctttct tgtttgcaga aagtgtagcc agaaaccctc
     1681 acgctgactt cccgttattg gcaaaaaaat gtttcatcct gtaccgcgcg gttaaccgct
     1741 gcggtcagac gctgcaactg ttgcgggaga ataatatagg gcggcatcag gtaaatcagt
     1801 ttgccaaaag gccggatcca taccccctgt tcgacaaaga atttttgcag cgccgccata
     1861 ttcaccggac gagtggtttc gaccacaccg attgccccca gtacgcgcac atcggcaacc
     1921 atttcggcat cacgggctgg tgccagttgc tcgcgcagct gcacttcaat agccgccacc
     1981 tgctgctgcc attcgccgga ttcgataatc gctaggctgg cgtttgctgc cgcgcaggcc
     2041 agcggattgc ccataaaagt tggcccatgc ataaagcagc cggcttcgcc gttactgatg
     2101 gtttctgcaa cctcgcgcgt ggtaagtgtg gcggaaaggg tcattgtgcc gccggttaag
     2161 gctttaccga ggcacaaaat gtccggcgcg atttctgcat attcacaggc aaacagtttg
     2221 ccggtacgac caaatccggt ggcgatctcg tcggcaatca gcaagatacc ttcgcgatcg
     2281 cacattttgc ggattcgttt taaccattcc ggatggtaca tgcgcatccc gcctgcgccc
     2341 tggacaatcg gctcaatgat caccgctgcg atttcatgac gatgcgccgc catcaggcgg
     2401 gcaaaaccaa ccatatcgcg ttcatcccat tcaccatcca tgcggctttg cggggcggga
     2461 gcaaacaagt tttctggcag atagcctttc cacaggctgt gcattgagtt gtccggatcg
     2521 cacaccgaca tcgcgccaaa ggtatcgcca tgataaccat tgcggaaggt cagaaaacgc
     2581 tggcgcgctt cgcctttggc ttgccagtac tgcaacgcca ttttcatcgc cacttccacc
     2641 gctacggaac cggagtccgc gagaaaaacg cactccagcg gttgcggcgt cattgccacc
     2701 agtttgcggc atagctcgat ggcgggcgca tgggtgatac cgccaaacat cacatgcgac
     2761 atggcatcaa tttgcgtctt catcgccgca ttaagctgcg ggtgattgta gccgtggatc
     2821 gccgcccacc aggacgacat accgtcaacc aggcgtctgc cgtcagacaa aattagctcg
     2881 caaccttcgg cgctcgccac cggataaacc ggcagagggg aggtcatgga tgtgtatggg
     2941 tgccagatat ggcgttggtc aaaggcaaga tcgtccgttg tcataatcga cttgtaaacc
     3001 aaattgaaaa gatttaggtt tacaagccta caccgaatta acaacaaaaa acacgttttg
     3061 gagaagcccc atggctcacc gcccacgctg gacattgtcg caagtcacag aattatttga
     3121 aaaaccgttg ctggatctgc tgtttgaagc gcagcaggtg catcgccagc atttcgaacc
     3181 tcgtcaggtg caggtcagca cgttgctgtc gattaagacc ggagcttgtc cggaagattg
     3241 caaatactgc ccgcaaagct cgcgctacaa aaccgggctg gaagccgagc ggttgatgga
     3301 agttgaacag gtgctggagt cggcgcgcaa agcgaaagcg gcaggatcga cgcgcttctg
     3361 tatgggcgcg gcatggaaga atccccacga acgcgatatg ccgtacctgg aacaaatggt
     3421 gcagggggta aaagcgatgg ggctggaggc gtgtatgacg ctgggcacgt tgagtgaatc
     3481 tcaggcgcag cgcctcgcga acgccgggct ggattactac aaccacaacc tggacacctc
     3541 gccggagttt tacggcaata tcatcaccac acgcacttat caggaacgcc tcgatacgct
     3601 ggaaaaagtg cgcgatgccg ggatcaaagt ctgctcaggt ggcattgtgg gcttaggcga
     3661 aacggtaaaa gatcgcgccg gattattgct gcaactggca aacctgccga cgccgccgga
     3721 aagcgtacca atcaacatgc tggtgaaggt gaaaggcacg ccgcttgccg ataacgatga
     3781 cgttgatgcc tttgatttta ttcgcaccat tgccgtagcg cggatcatga tgccgacctc
     3841 ttacgtgcgc ctttctgccg gacgcgagca gatgaacgaa cagactcagg cgatgtgctt
     3901 tatggcaggc gcaaactcga ttttctacgg ttgcaaactg ctgaccacgc cgaatccgga
     3961 agaagataaa gacctgcaac tgttccgcaa actggggcta aatccgcagc aaactgccgt
     4021 gctggcaggg gataacgaac aacagcaacg tcttggacag gcgctgatga ccccggacac
     4081 cgacgaatat tacaacgcgg cagcattatg agctggcagg agaaaatcaa cgcggcgctc
     4141 gatgcgcggc gtgctgccga tgccctgcgt cgccgttatc cggtggcgca aggagccgga
     4201 cgctggctgg tggcggatga tcgccagtat ctgaactttt ccagtaacga ttatttaggt
     4261 ttaagccatc atccgcaaat tatccgtgcc tggaagcagg gggcggagca atttggcatc
     4321 ggtagcggcg gctccggtca cgtcagcggt tatagcgtgg tgcatcaggc gctggaagaa
     4381 gaactggccg agtggcttgg ctattcgcgg gcgctgctgt ttatctctgg ttttgccgcg
     4441 aatcaggcag tcattgccgc gatgatggcg aaagaggacc gtattgttgc cgaccggctt
     4501 agccatgcct cattgctgga ggctgccagt ttaagcccgt cgcagcttcg ccgttttact
     4561 cataacgatg tcgctcatct ggcgcgactg cttgcttccc cctgtccggg gcagcaactg
     4621 gtggtgacag aaggcgtgtt cagcatggac ggcgatagcg cgccactggc ggaaatccag
     4681 caggtaacgc aacagcacaa tggctggttg atggtcgatg atgcccacgg cacgggcgtt
     4741 atcggggagc aggggcgcgg cagctgctgg ctgcaaaagg taaaaccaga attgctggta
     4801 gtgacttttg gcaaaggatt tggcgtcagt ggggctgcgg tgctttgctc aagtacggtg
     4861 gcggattatc tgctgcaatt cgcccgccac cttatctaca gcaccagtat gccgcccgct
     4921 caggcgcagg cattacgtgc gtcgctggcg gtcattcgcc gtgatgaggg ggatgcacgg
     4981 cgcgaaaaac tggtgtcact cattgcgcgt tttcgtgccg gagtacagga tttgccgttt
     5041 acgctggctg attcatgcag cgccatccag ccattgatcg tcggtgataa cagccgtgcg
     5101 ttacaactgg cagaaaaact gcgccagcaa ggctgctggg tcacggcgat tcgcccgcca
     5161 accgtacccg ctggtactgc gcgactgcgc ttaacgctaa ccgctgcgca tgaaatgcag
     5221 gatatcgacc gtctgctgga ggtgctgcat ggcaacggtt aataaacaag ccattgcagc
     5281 ggcatttggt cgggcagccg cacactatga gcaacatgca gatctacagc gccagagtgc
     5341 tgacgcctta ctggcaatgc ttccacagcg taaatacacc cacgtactgg acgcgggttg
     5401 tggacctggc tggatgagcc gccactggcg ggaacgtcac gcgcaggtga cggcgttaga
     5461 tctctcgccg ccaatgcttg cgcaagcccg ccataaagat gccgcagatc attatctggc
     5521 aggagatatc gaatccctgc cgttagcgac tgcgacgttc gatcttgcat ggagcaatct
     5581 cgcggtgcag tggtgcggta atttatccac ggcactccgc gagctgtatc gggtggtgcg
     5641 ccccggaggc gtggtcgcgt ttaccacgct ggtgcaggga tcgttacccg aactgcatca
     5701 ggcgcggcag gcggtggacg agcgtccgca tgctaatcgc tttttaccgc cagatgaaat
     5761 cgaagagtcg ctgaacggcg tgcgttatca acatcatatt cagctcatca cactatggtt
     5821 tgatgatgcg ctcagtgcca tgcgttcgct gaaaggcatc ggtgctacgc atcttcatga
     5881 agggcgcgac ccgcgaatat taacgcgttc gcagttgcag cgattgcaac tggcctggcc
     5941 gcaacaacag gggcgatatc ctctgacgta tcatcttttt ttgggagtga ttgctcgtga
     6001 gtaaacgtta ttttgtcacc ggaacggata ccgaagtggg gaaaaccgtc gccagttgtg
     6061 cacttttaca agccgcgaag gcagtaggtt atcggacggc aggttataaa ccggtcgcct
     6121 ctggcagcga aaagaccccg gaaggcttac gtaatagtga cgcgctggcg ttacagcaca
     6181 acagcagcct gcagctggat tacgcaacgg taaatcctta caccttcgca gaatccacct
     6241 cgccgcacat catcagcgcc caagagggca ggccgataga atcattggta atgagcgccg
     6301 gattacgcgc gcttgaacaa caggctgact gggtgttagt ggaaggtgct ggcggctggt
     6361 ttacgccgct ttctgacact ttcacttttg cagattgggt aacacaggaa caactgccgg
     6421 tgatactggt agttggtgtg aaactcggct gtattaatca cgcgatgttg actgcgcagg
     6481 caatacaaca cgccggactg actctggcgg gttgggtggc gaacgatgtt acgcctccgg
     6541 gaaaacgtca cgctgaatat atgaccacgc tcacccgcat gattcccgcg ccgctgctgg
     6601 gagagatccc ctggcttgca gaaaatccag aaaatgcggc aaccggaaag tacataaacc
     6661 ttgccttgtt gtagccattc tgtatttggt taaattgcga gcgagatcgc gtcttcgatt
     6721 gactgcaatt taaccaatta aattctaaaa taatcacgaa aaaaatttta cttccgcctc
     6781 atgcggcgaa tgtgggaatt gcccaggcgg cgggggatag gggctggaga cagttatcca
     6841 ctattcctgt ggataaccat gtgtattaga gttagaaaac acgaggcaag cgagagaata
     6901 cgcggcttgc acgcgaattg gcgttaaaga cggctcaaag aaatatcttt tattttttaa
     6961 ctggttagat aaatgcaatg gcagtcactg aacaggcatc tcttgccata aaactgtcat
     7021 cactcatctt gacaaatgtt aaaaaagccg ttgctttggg gataacccgg taaggctgga
     7081 gttttatctc gccacagagt aaattttgct catgattgac agcggagttt acgctgtatc
     7141 agaaatatta tggtgatgaa ctgttttttt atccagtata atttgttggc ataattaagt
     7201 acgacgagta aaattacata cctgcccgcc caactccttc aggtagcgac tcatgagtaa
     7261 accgttcaaa ctgaattccg cttttaaacc ttctggcgat cagccagagg cgattcgacg
     7321 tctcgaagag gggctggaag atggcctggc gcaccagacg ttacttggcg tgactggctc
     7381 cgggaaaacc ttcaccattg ccaatgtcat tgctgacctt cagcgcccaa ccatggtact
     7441 tgcgcccaac aaaacgctgg cggcccagct gtatggcgaa atgaaagagt tcttcccgga
     7501 aaacgcggtg gagtatttcg tctcctacta cgactactat cagccggaag cctatgtacc
     7561 gagttccgac accttcattg agaaagatgc ctcggttaac gaacatattg agcagatgcg
     7621 tttgtccgcc accaaagcga tgctggagcg gcgtgatgtg attgtagtgg cgtctgtttc
     7681 cgcgatttat ggtctgggcg atcctgattt atatctcaag atgatgctcc atctcacggt
     7741 cggtatgatt atcgatcagc gcgcgattct gcgccgactg gcggagctgc aatacgctcg
     7801 taatgatcaa gcattccagc gtggtacttt ccgcgttcgt ggcgaggtga tagatatctt
     7861 cccggcagaa tcggatgaca ttgcacttcg cgtggaactg tttgacgagg aagtggaacg
     7921 attgtcgtta tttgacccgc tgaccgggca gattgtttcc actattccac gttttaccat
     7981 ctacccgaaa acgcactacg tcacaccgcg cgagcgcatc gtacaggcga tggaggagat
     8041 caaagaagaa ctggccgcca gacgcaaagt gctgttggaa aacaacaaac tgctggaaga
     8101 gcagcggctg acccagcgta cccagtttga tctggagatg atggacgagc tgggctactg
     8161 ttcggggatt gaaaactact cgcgcttcct ctccggtcgt ggaccgggtg agccaccgcc
     8221 gacgctgttt gattacctgc ctgccgatgg gctgctggtc gtcgatgaat ctcacgtcac
     8281 cattccacaa attggcggca tgtatcgcgg tgaccgggcg cgtaaagaga cactggtgga
     8341 gtacggcttc cgcctgccat cagcgctgga taaccgtccg cttaagtttg aagagttcga
     8401 agcattagcg ccgcaaacca tctatgtttc ggcgacgccg ggtaattacg agctggaaaa
     8461 atccggcggc gatgtggtgg atcaggtggt gcgtccaacc ggattgcttg acccgattat
     8521 cgaagtgcgg ccggtggcga cacaggttga tgatcttctt tcggagattc gtcagcgagc
     8581 ggcaattaac gaacgcgtgc tggtcaccac actgaccaag cgaatggcgg aagatcttac
     8641 cgaatatctc gaagaacacg gtgagcgcgt gcgttatctt cactcagata tcgacaccgt
     8701 cgagcgtatg gagattatcc gcgacttgcg tctgggtgag ttcgacgtat tggtagggat
     8761 caacttactg cgtgaaggtc tggatatgcc ggaagtgtcg ctggtggcga tcctcgacgc
     8821 tgacaaagaa ggcttcctgc gttccgaacg ttcgttgatc cagaccattg gtcgtgcggc
     8881 acgtaacgtt aacggtaaag cgattctcta cggcgatagg agcactccat caatggcgaa
     8941 agcgattggc gaaaccgaac gtcgccgtga gaaacagcag aagtacaacg aggaacacgg
     9001 cattacgccg caaggcttga acaagaaagt ggtcgatatc ctggcgctgg ggcagaacat
     9061 tgccaaaacc aaagcgaagg gcagaggaaa atcgcgcccg attgttgagc cggataatgt
     9121 gccgatggat atgttgccga aagcgttgca gcagaaaatc catgaactgg aagggctgat
     9181 gatgcaacac gcgcagaatc tggagttcga agaagcggca caaattcgtg accagttgca
     9241 tcagttgcgt gagttgttta ttgccgcatc gtaacaggat agcgaagaag actgatgaca
     9301 aacggaaaac agtctgatgc gctacgctta tcaggcctac attttctccg caatatattg
     9361 aatttgcgcg gtttgtaggc cggataaggc gttcacgccg catccggcat aaacaacgcg
     9421 cactttgtct tcaataggaa gccggaattt tccttcggat ttccgttaac ctaaagcctg
     9481 taacgccttt tccagcgcgt tatgtaacaa ctggcggtca tgacgatacg gaatatcgct
     9541 ggcctccagt acctcctgga tcacaatccg ctctttcacc gccgagacat ccacttttgg
     9601 cccgacgatg accgcatcaa tgaccttttt accaacatac tgctccataa ttgccagctt
     9661 gctttccagc ttcaaattag ccgcaggtaa actcaactca cgccccagat tgccgatata
     9721 aaccatcggc gctggcgtgc ggcgtaatgc ctgggcgatt tccttcagca gcagaattgg
     9781 catcaggctg gtataaaaac tgccaggccc aataatgatg agatccgctt cattgatagc
     9841 gtgaaccgcc tcacgcgttg cgggtacatt aggcgttaac aataactctt gaatcggcgt
     9901 agttaactgg tcgatattga cctcgccgta aacttcatgc ccctgatcgt caatcgccat
     9961 cagatcaaca ggatgctctg acattggaat caaatgcgta tccactttca gcagattacg
    10021 aattaaattg atggcttcca gaggccgcac gctaaggtga tccagcgcct ttaacatcaa
    10081 gtttccgaga ttatgaccgg aaagttcgcc attgccgcca aaacggtatt caaacatcgc
    10141 ggaggcgacg ttcggttccg ttatcagctg gttgaggcag ttgcgcatat cgccccaagc
    10201 aatgccgcct tctgaacggc gaatacgccc cgtcgagcca ccattatccg tggtggtgac
    10261 gatacccgtt aaacgagaac ccaaagacga aagtgatgag agaacgcgtc ccagtccatg
    10321 ccctccgccg agagcaacga cacgatcaag atcagccagc gtacgattgc gcatatagtt
    10381 tcctggagtc agattatccg cgctacagta gcgcaaattg cggggaaaca gcaattaacc
    10441 tgccaatatt gaggatacaa actattttct tctatctcta aaggacgatg cacgctatgc
    10501 ctccctgatg atgtatatca aagtaaaacc gccattttcc cttattctgt agcgaaatag
    10561 cacgatcatg acgctatata tatgattaca tagcgaaagt gtggatggta aaaatctcat
    10621 ttacacgcta gtatcggctt aaccactaaa cactctagcc tctgcacctg ggtcaactga
    10681 tacggtgctt tggccgtgac aatgctcgta aagattgcca ccagggcgaa ggaagaaatg
    10741 acttcgcctc ccgtatctgg aaaggtgtac atggcttcac aactgactga tgtatttgcg
    10801 cgtaagtttt actacttgcg cctgtcgatt accgatgtgt gtaactttcg ttgcacctac
    10861 tgcctgccgg atggctacaa accgagcggc gtcaccaata aaggctttct taccgtcgat
    10921 gaaattcgcc gggttacgcg cgccttcgcc agtctgggca ccgaaaaagt gcgcctgaca
    10981 ggaggagagc cgtctttacg ccgcgacttt accgatatca tcgccgctgt gcgggaaaac
    11041 gacgctatcc gccagattgc ggtcaccacc aatggttacc gtctggaacg cgatgtggcg
    11101 aactggcgcg atgcgggact tactggcatt aacgtcagtg tcgacagttt ggacgcccgc
    11161 cagtttcacg ctattaccgg gcaggataaa ttcaaccagg tcatggcagg gattgatgct
    11221 gcatttgagg ccggttttga gaaggtcaaa gtcaataccg tgctgatgcg tgatgttaat
    11281 catcaccagc tcgacacctt tctgaactgg atccagcatc gccctatcca gctgcgtttc
    11341 atcgaactga tggaaacggg cgagggcagc gagctcttcc gtaagcatca catctctggt
    11401 caggttctgc gtgacgagct actgcgtcgc ggctggatcc accaattacg tcaacgcagc
    11461 gacggtcccg cgcaagtctt ttgccatcca gattacgccg gagagattgg ccttatcatg
    11521 ccgtatgaaa aagacttctg cgccacttgc aaccgcctgc gcgtttcctc cattggtaaa
    11581 ctccatctct gcctgtttgg tgaaggcggc gttaacctgc gcgatctgct ggaaggcgat
    11641 acccagcaac aggcgctgga agcgcgtatt tcagcggcgc tgcgggagaa gaaacagacc
    11701 catttcctgc atcaaaacaa caccggtatt acgcaaaact tatcgtacat tggcggctaa
    11761 aacgtcaaaa ggagagatca gatgagtcag gtaagcactg aatttatccc gacccgtatt
    11821 gctattctta cggtttctaa tcgtcgcggt gaagaagacg atacctccgg tcactatctg
    11881 cgcgattcgg cgcaagaagc gggccatcac gttgtcgatg aagccattgt gaaagaaaac
    11941 cgctacgcta ttcgcgctca ggtatctgcg tggatcgcca gcgacgatgt acaagtggta
    12001 ttgattacgg gtggtactgg cctgacggaa ggtgatcagg ctcccgaagc gctgctgccg
    12061 ttgttcgacc gtgaggttga aggttttggt gaagtgttcc gtatgttgtc gtttgaagag
    12121 attggcactt ccacgttgca atctcgtgcg gtagcgggcg tcgctaacaa aacgctgatt
    12181 ttcgccatgc cgggttcgac caaagcgtgc cgtaccgcat gggaaaatat catcgcgccg
    12241 cagctggatg cccgtacgcg tccgtgtaat ttccatccac atttgaagaa ataagtatgt
    12301 cgcaactgac ccatatcaac gccgctggcg aagcgcacat ggtggatgtc tccgccaaag
    12361 cggaaaccgt gcgtgaagcg cgggcggaag cctttgtcac catgcgcagc gagacgctgg
    12421 cgatgattat tgatggtcgc caccacaaag gcgacgtatt tgccactgcg cgtattgccg
    12481 gtattcaggc ggcaaaacgc acttgggatc tgatcccgct gtgtcacccg ctgatgctca
    12541 gcaaagttga agtcaattta caggccgagc cggagcacaa tcgggtgcgt atagaaacct
    12601 tatgccgcct gaccgggaaa accggtgtcg aaatggaagc gttaaccgcg gcctccgtgg
    12661 cggcgctgac catttatgac atgtgcaaag cggtgcaaaa agatatggtg attggtccgg
    12721 tacgtttgct ggcgaagagc ggcggcaagt cgggtgactt taaggtggaa gcggatgatt
    12781 aaagttcttt ttttcgccca ggtgcgcgag ttggtgggaa cagatgcaac cgaagtggct
    12841 gcggattacc caaccgttga agcgttacgc cagcacctgg ctgcgcagag cgatcgctgg
    12901 gcgctggcgc tggaagatgg caaattgctg gctgccgtca accagacgct ggtgagtttt
    12961 gaccatccgc tgactgacgg cgacgaagta gctttcttcc cgccggtaac cggaggttaa
    13021 gatggcagaa accaaaattg ttgtcggccc gcagccgttc agcgtaggag aagagtaccc
    13081 gtggctggcg gagcgtgacg aagacggtgc ggtagtcacc ttcaccggaa aggtgcgcaa
    13141 ccataacctg ggcgacagcg tcaaagcatt aaccctcgaa cactatccgg ggatgactga
    13201 aaaagcactg gcagaaattg ttgatgaagc gcgtaaccgc tggccgctgg ggcgcgtcac
    13261 ggtgattcac cgcatcgggg aattatggcc gggcgatgaa atcgtttttg tcggtgtcac
    13321 cagtgcgcat cgcagcagtg cgtttgaagc cgggcagttt attatggatt atctcaaaac
    13381 ccgcgcaccg ttctggaagc gcgaagccac gccggaaggc gaccgctggg ttgaagcccg
    13441 ggagagcgat ctgcaggcgg caaaacgctg gtagtttttt gttagccgga taaggcaccg
    13501 ctgcgtccgg caatttcacc gctcagtaca tgcgtttacg ctgcgtcgat gcacagcctc
    13561 atcattttgc agtatcctta agatattcct tatatcttca ggagatcgtc atggacagat
    13621 tcccacgttc tgattcaatc gtacaaccct gggctggctt gcaaacttat atggcgcaag
    13681 tctatggctg gatgaccgtt ggcttgttgc tgaccgcatt tgttgcctgg tatgcggcta
    13741 attccgcggc cgtgatggag ctgttgttca ctaaccgtgt ctttttaatc ggtctgatca
    13801 tcgcgcaatt agcattggtt attgtgttat cagcgatgat tcaaaagctg agcgcaggtg
    13861 taacgacgat gctctttatg ctttattcgg cgctgacggg tcttacgctt tccagtatat
    13921 tcattgtcta taccgctgct tctatcgcca gtactttcgt cgttactgcc gggatgttcg
    13981 gcgcaatgag cctgtacggt tacaccacga agcgcgattt aagtggcttt ggcaatatgc
    14041 tgtttatggc gttaatcggc attgtgctgg catcgctggt caacttctgg ctgaaaagcg
    14101 aagcattgat gtgggcagtt acctacatcg gcgtgattgt ctttgtcgga ttgacggcgt
    14161 atgacactca gaaattgaaa aatatgggtg agcagattga tacccgcgac acgtcgaacc
    14221 tgcgcaaata ttccattctt ggtgcgttaa ccttgtatct ggacttcatc aacctgttcc
    14281 tgatgttgtt acggatcttc ggcaaccgcc gttaattctt tttcaccatc actatcccgg
    14341 ccatgtgtcg ggattttttt cttactaaaa acgacactta acaagtacca ggtataaaat
    14401 atgccttatg agcgagcgga atcgttttcc gtatccataa atacaaaagt attttgtatg
    14461 cgtggtttta aatttattga tggtgaatat taatgttttt tctaaggaca actaaaagaa
    14521 tggaatcata ctcgcaaaac agcaataaat tagattttca gcacgaggcc aggatattaa
    14581 acggtatatg gctcattacc gctttaggtt tggtggcaac cgcaggacta gcctggggag
    14641 ctaagtatct cgaaattacg gcaaccaaat atgattcacc accaatgtat gtcgccatag
    14701 ggttattatt actttgtatg tatggcttaa gtaaggatat caacaagata aatgccgcca
    14761 tcgcgggcgt aatatatctg tttttactct ctttggtggc gattgtcgtt gcaagtttag
    14821 ttcctgtata tgccattatc atcgtgttca gcactgcggg cgcgatgttt ttaatcagta
    14881 tgctggccgg tttattattt aatgttgatc ctggttctca ccgttttatc attatgatga
    14941 cgttgacagg gttggccctg gtaatcatcg tgaatgcggc attaatgagt gaacggccca
    15001 tttgggtaat aagttgctta atgattgtgt tatggccagg cattatctcg catggacgaa
    15061 ataagctcct tgaactggca ggaaaatgcc atagtgaaga gttgtggagt ccggttcgtt
    15121 gcgcttttac aggtgcatta acactctatt actattttat cggcttcttt gggatacttg
    15181 ccgcgatagc tataacgctt gtctggcaaa ggcatacgcg tttttttcat tagtcgtaaa
    15241 tgcagatact aaagaactta actaaacttc acatcgccgc ttcatttttc gcccgtagct
    15301 tcttcgcttg gctttccagt agcaggtaac agatcagcgc cagcagcagc gggataaagt
    15361 aatacaggac gcgataagcg agcagggcgg cgataatgct ccctttcgag gtatgctccc
    15421 cagccagtag tgcgataaac accgcttcca gcacaccgat ccccgccgga atatgcacga
    15481 tgacgccagc aatactacta accagtaaca cgcccagtac aaagaaatag ttcacgcttt
    15541 gaccaagtaa cagccagata atcgccccca ttaccatcca gttaacgctg gaaatcagca
    15601 tctgagcgag ggcgaatttc catgaaggca gcaccagctt ttgccctttg atggtcatat
    15661 ggcggtgctt cgcgaaagcg caaaaccaca aataaaccgc gataatcatc agtaagccaa
    15721 tgccgagaat acgcagcgta gtttgatcga cataccagta atccggcaac tccaccacgc
    15781 ctgcggtaaa gataatccct gccagtaaaa tatagcccag ccagttggtg gtaatactga
    15841 gcgaggaaat ccgcgtaata gtgctgcccg gtaaccctag tcgggagtac aaacgatagc
    15901 gcataccaat gccgccgacc caggtactga gcgtcaggtt gaaggcgtag cagataaacg
    15961 acaccagcat cacctggcgc ttcgccagtt tgtgaccgca gtaaaagcgg gcgagcaggt
    16021 catagcagcc gtaaatcaga tagctgacga ccaccagccc gaccgcacta agcagcgcaa
    16081 cgcgattgta gtcgcggatg accttccaga cctcttccca gtccactttt ttggcgtaga
    16141 ccaccagcaa cacaatcacc gcgataaaaa acagccaggt gaggatcttc tttgctaagc
    16201 gccagcgcgg gtgtgattta ctcatcaggg gtttaccccc gtgttttcag tttctacccg
    16261 atcctgcgtt tccattgtcg gttgtgcggg gggatcaacc tgcgtcagac gtggtgtgtg
    16321 tgccggaagc cagccaacca gcgccgggaa gtggcgtaaa aagtggaacg ccagcacgct
    16381 tttggtcagg ttccaccagg tgcgtttggg cagcatggtt tcatccacct gctgacaatc
    16441 tgcggcaata atgccgttca gattatcgcg cagcgtctgg ttaaaatgac gatcgtggat
    16501 gatgacattt gcttcgagat tcagtgacaa actgagcgga tcgagattac tggaccctac
    16561 tgtcgcccag tgatcgtcca tcaatgccac tttgccgtgg agcgggcggc ggcggtactc
    16621 aaaaacctga acgccgcctt taaccagata gttatacagc aagcgcgcac cgactctgac
    16681 aatcggcata tccggttcgc cctgaatgat cagtttgatc cgcaccccgc gccgtgccgc
    16741 tttacgcaag gcgtgtaaaa atcgatagcc ggggaagaag taggcgttgg cgataatcac
    16801 ttcccgctgc gcctgagtga gcattttcag ataatggcgt tcaatatcat cgcgatgttc
    16861 ttcgttatcg cgccagacca gcaatacctg cgcttctccc ggctggcggt tctcttccgc
    16921 tttgtgatga cgtcgccacc agcgtcgtgc cgcgctctgt ccaggcaggt tttccagctc
    16981 aaactggaga atatcttcga caatcggccc ttcaaggcgt accgcgtaat cctgtttagc
    17041 ctctggaccg tagctggaca tatgctcggc ggagtaattc agcccgccaa taaaggcgat
    17101 acgcgcgtcg atcaccacaa ttttgcgatg catccggcga aacacattgg tgcgcatacc
    17161 aaaaaggcga gggcggggat cgtagtagcg gaacactacg ccagctgccg tcagttcatt
    17221 gacaaactca tcgctgagat ccggcgaacc gtagccatcc agcaagactt ccgctttaac
    17281 cccgcgttgc gctgctgcca gtagtgccgc atgcagctgt ttgccgacgt tatcctcaaa
    17341 ccagataaac gtttcaagaa tgatgcgttc ttgtgcctcg ccaatcgcct taaacaccgc
    17401 gggataatat tgctcgccgt tttccagcaa ctggatctta ttgccttcgc gccagctaca
    17461 tttcataaat gaatctccgc acttaaaggg gcatgatcag aaaggtgtcg ccatgtccgc
    17521 agcggcaacg cggttggcgc gctggcgctg gcatttttga cgtagatcct gtccagtcgt
    17581 agtagaggaa attgcaccgg aaacgtgcgc gccgggcgtc cgtgggcgcg ggtaaaaatc
    17641 tcatccagtc cggcctgcac ttttaacgga tgattagctt tttgccgcca gtcattgaaa
    17701 tcacccgcca ccaataccgg ttcgccgtcc ggtagctcat tcacccattc ggcgagcatc
    17761 gcaagctgcg cctgacggtg cgcctcacgc aggcccagat gtacgcacat cacatgaatc
    17821 gcttttccgg tcatcggcgg cacaatgcgg cagtagagca cgccgcgctt ttccgcacca
    17881 tcgaccgaaa catcgcgatt ctcataatgt tcaatgggat aacgcgacag tacggcgttg
    17941 ccgtgatgcc cttccgggta tacggcattg cgaccgtagg caaaatcgct ccacatagtg
    18001 tcggcgagaa actcgtagtg cgaggtatcg ggccagtttt ccacatgcag cggatgaact
    18061 tcgtgcgcgc ccatcacttc ctgcaggcaa acaatatcgg cgctgacggt acgcacggca
    18121 tcgcgaagtt ccggcaaaat gaagcgtcgg ttaaacgcgg taaagccttt gtgaatgttg
    18181 atggtgagca ccttgaacga aaattgttgt gtttgatcgg gcataatttt cctgtctttg
    18241 cctcttatct cattgaaata gtgtagtcgg cgtcacaaaa aggtgcggtc ttacggaatt
    18301 ttccgtaaag ttcggtactc tgagtaagta gagataaatt cttcaggaga gaagccatga
    18361 agtggcaaca acgtgttcgt gtcgcaacgg gtctaagttg ctggcagatt atgttgcatt
    18421 tactggtagt ggcgctgctg gtggtgggct ggatgagtaa gactctggtt cacgtcggcg
    18481 tgggattatg cgcactgtat tgtgtcacgg tagtgatgat gctggtgttt cagcgccacc
    18541 ccgagcaacg ctggcgtgag gtggcagacg tgctggaaga gctgaccacg acctggtatt
    18601 ttggcgcagc gctgattgtg ctgtggctgt tgtcccgcgt actggaaaac aactttttgc
    18661 tggcaattgc agggctggcg atccttgctg gcccggcggt agtgtctttg ctggcgaaag
    18721 ataagaagtt acatcacctt acgtctaaac atcgcgtacg ccgctgaccc tgtcgtggcc
    18781 gttatcacca gtagcggcca caaactattc cacacaatat ccagactcgc atccttcaaa
    18841 taaatctgct tggtaatgtc cgtaaagtgg cgaatagggt taatccacgt cagattttgc
    18901 agccacactg gcatgttttc caccggcgaa acgtaaccgg aaaggagaat ggcgggcatc
    18961 ataaaaacaa acacgccgat aaacgcctgc tgttgtgtcg agcagagtga tgaaatcaac
    19021 agaccgaatc ccaccagcga taaaccgtaa atcaccatcg taaagtagaa cagcgctagc
    19081 gatccggcga aggggatttg atacgcccag ataccaatcg ccagcacaat ggtggcctgg
    19141 aaggtggcga caattaacgc cggtacggct ttgccgatga agatctgcca ggtggtgagc
    19201 ggcgaaacca gtagctgatc gagcgtacct tgttcacgtt cgcgggcgac ggaaagtgaa
    19261 gtgacgatca ttacgccgat agtggtgatc atggcgatca gcgacggcac cacaaaccat
    19321 ttgtagtcga gattcgggtt ataccagttg cgtaccacca gctcgctgtt gttgggtttc
    19381 ggttttcctt ccagcagctc ctgctgataa tttttgacga tctgttgcag gtagttggcg
    19441 gcaatttgcg cactgttgga gttacgcccg tcgaggatca actgcaacgg cgcggtctgg
    19501 aaggtatcca gtttgcgcga gaagtcagcc gggaaacgca ccagtagtaa cgccttttgt
    19561 gtgtcgatgg tcgggcggat ctcctgtggg cttttcagca gcagcacatg tgtaaaggcg
    19621 ctggcgcggg caaaacgttg agtaagttcc accgaatgct cgccgttatc ttcatcgtag
    19681 atggcgatgg tggcgttagt cacttccagc gtggcggcga acgggaacag gatcacctga
    19741 attagcacgg gtaaaatcag aatcgcgcgg gtttgtggtt cgcgcagcaa cgactgcaac
    19801 tctttgcgga ttaacgtcca taagcgatga aacatgctct tctccctaat ccagccgacg
    19861 tttggttttc agccacgtca ggccgataaa catcaccgcc gaagcgatca aaaacagcac
    19921 gtttaccacc agcaccactg gaatattccc ggcgaggaac aggctttgca gggtgctgac
    19981 gaaataacga gcgggaataa tgtacgtcac cgcgcggatc accgcgggca tactgtcgat
    20041 ctgaaaaata aagccggaaa gcataatcga cggcagaaaa gcggcgttca gggcgacctg
    20101 agcggcattg aactggttgc gggtaatcgt ggaaatcagc agccccattc ccagggtact
    20161 gagtaaaaac aggctggaga taaaaaacag aatcagcagc gaaccgcgat acggcacgcc
    20221 gagaataaac actgacacca gcatacacag caacatcgcc agcatcccga gaaagtaata
    20281 agggatcagc ttacacagca gcagttccgt gcgggtaatc tccgtagaga gcagagcctc
    20341 catggtgccg cgttcccatt ctcgcgccac caccagcgag gtgagaatcg cgccgatgac
    20401 cgtcatgata atggtcaccg cacccgggat aataaagtgc tggcttatcg ccgccgggtt
    20461 aaaccagtag cgggtttgta catcaataag cggttcaaaa gtctgcccgt tgtcctccgc
    20521 tcgctgcatt tgccagatct gccagatccc ttcgacatac ccctgcacaa agttagcggt
    20581 attcggctca ctgccgtcgg tgatcacctg aatcggtgcg gtggcgttgg cgcgctccat
    20641 ctgttcggcg aaatccacgg gaataaccac cagaccgcga attttccccg actgcatttt
    20701 ggcgatcagt tcctgacggt tatcgctgat ggtggcgtcg atgtagggcg aaccggtcat
    20761 ggtgtgggtg aaatccagcg ccgcttcgct acgctgttcc agcaaaatcc cgacccgcag
    20821 cttgctggag tcgaggttaa tgccgtaacc aaaaataaac agcagtagca gtgggatcac
    20881 taccgcaatc agccagctac tcggatcgcg aacgatctgc cgcgtctctt tgacgcacag
    20941 cgcccgcacg cgatgccagg aaaggatcgg gttactcatt gctatgctcc ttatcccagt
    21001 cgtggatcaa ctgaataaag gcttgctcca tggtgggatc gggttgctca tcgttagctg
    21061 actgtgcttt caaatcgtcc ggcgtaccgc tggcgattaa tttcccacgg tacaccaggc
    21121 cgatgcggtc gcaatattcc gcttcatcca taaagtgggt ggtgaccatc accgtgacgc
    21181 ctttctctac catgctgttg atgtgcagcc aaaattcacg gcgggtgagg gggtcaacgc
    21241 cggaagtcgg ttcgtcgaga aagagaatat ccggttcatg catcagcgaa caggccagcg
    21301 ccagccgctg tttaaaacct aatggcagtt catcggtggc gtgtgaggca atacttttca
    21361 ggccaaatgc ctcgctcata cgggagattt tttcgttctg cgcccgaccg cgtaagccat
    21421 acacaccaga gaaaaagcgt aaattctgtt cgaccgtcag gttgccgtag agcgaaaatt
    21481 tttgcgccat atagccgaga tgctggcgcg ctttaccgga actctctttc agatccatcc
    21541 ccagcaccag cgcctggccg gaagtcggca ccagcaaacc gcacatcatc ttaaaggtgg
    21601 tcgatttacc cgcgccgttt ggccccagca aaccaaaaat ctccccacgt ttaacggcaa
    21661 agttgacgtg atcggtggcg gcaaaatcac caaatttctt ggtcagttct ttcgcttcga
    21721 tcaccgtctc gccgggtgtg ccttctaccg tatgcaatat tgcacccagc ggcgattctg
    21781 aggttccggc accgcccagc aaatcaataa acgcatcttc aaaacgcggc gtagtttcgt
    21841 tgatgttgat ttccggcatc ccgtcggcat ggcgaatatc gtctggtgtg gcctcttttt
    21901 tgaggatcag acgtaccgat ttcccctgaa tcatgccatc gctgacctgc ggcagtttca
    21961 aggcgcgttg caacagtttg cggttgccct cgtgtggact ggtcatcaga aagctgcgtc
    22021 cggccatggt ttgtgtcagg gcttttggtt ctccctgata cagcaactcg ccttcgttca
    22081 tcagtaacac gtcacggcac tgctcggctt cgtcgagata cgaggtactc cagaggatta
    22141 acatcccttc gcccgccagc tcatgcacca tctgccacag ttcgcgccgt gagatagggt
    22201 caacgccgac gccgggttca tcgagcagca acactttcgg ttcgcccacc agggtacagg
    22261 ccaggccgag tttttgtttc atcccaccgg agagcttgcc cgccaggcgt ccggtaaacg
    22321 gcccaagaga cgtaaactcc agcaggcgag caaaagtttg cttacgtgcc tcgccggtga
    22381 cgctgcgcaa atccgcgtac agattgaggt tctccatcac cgtgagatct tcatacagac
    22441 caaatttctg cggcatatag ccgagcacgg cgtgcagcgc gccgtcgttt ttgatcggat
    22501 caaagccaat caccgtggca ctgccgctgt cgggtttcag taatcccgcc aacatccgca
    22561 tcagtgtggt tttacctgca ccgtccggcc ccaccaaccc cgtcacataa ccggcgtgaa
    22621 tggtacaatc gagcggcgcg acggcgggct tgtccatgcc cggaaagcgt ttttccaggc
    22681 cgttcagcgt gataacggca tcattcatgt cctgcctcgt caccgaattg taccgtcact
    22741 ggcattccct ggcgtaacgc atcatcggcg tcggtcacca caatacgcag gcgatagacg
    22801 aggtcggtac gcagatccgg tgtttcgacg gttttcgggg taaattcagc agtcggcgaa
    22861 acgaaaccaa tctgcccgtg atacggcttg tccgggcgac catcggtata aagcagcact
    22921 ttgcgccccg gctgggcctg gtcaagattc cgttcatcaa cataagcgcg cacccacacc
    22981 ggacgcgtta gtgaaacggt aaacaccgtg ccaccttcat tgagaaccgt gcctggctct
    23041 accgcgcgcg ttaacagcgt gccatcagac ggtgctatca acgttgagtc ctgtaaattc
    23101 agctccgcct gcgccagttg cgcctgcgcc tgttcgaggc tggctttcgc ctgagcgatg
    23161 tcctgttcac ggttaccgga acggtactga cgcaatttat cctgtgctga tttcagcgtt
    23221 gcctgtgcct ggtcgcgcga ggagcgggca ttttccaggt catttgccga aatggtgcgg
    23281 cttttccaca gtccttgctg gcggttatag aagttctgcg catagtcata ggcggcttgc
    23341 gcctgtttca ccgctgcggc ggcctgagcg atttcttcat cgcgataccc ggcaagcatc
    23401 agatcatact gcgcctgtgc caccgagacg cccgctttcg cctgcatcag ggcaatctca
    23461 tacggcttgt gatccagttc gcccagcacc tggcccgctt tgatagcatc accttcgtcc
    23521 accgccagcg attcaacgcg ccccccaaca cggaaactaa gattcaccgt acgaatatcc
    23581 acgttgccat acagcgtcag gccgttatcc tggcggcttt gataccacca gtagcctccg
    23641 gcaaccacgg cggcaagtac cactaccgcc agtccgatca cgacaggttt tttcatcact
    23701 acaaactcct ttgcgataat ccttgcaaaa tcaggtcgat atgacaggtc accgtctggt
    23761 tgatcagttc ggtcttttct tcatcgaacg cggtccagcc ggtacgtaac agaattgttt
    23821 ctttgccaag acggaacgcc agaatctcac caatcaacgc atgggtatga aggatcatgc
    23881 gggtgtcatt agcgtcgcag ccggtccagg cggcaatcag gcgtgtcagg tggctgtgta
    23941 gcggactaat cacctgttcg tgcaccaggt ggtaggctgc cgtgggagag agctgctcac
    24001 gggagataaa cttgctgagg ttgacggtgt catcctgggt gagcagctta atcatgttcc
    24061 tgcaggcgcg aaggatcagt tcacggatgg cagcccgatc aggctgtggt tgggcgaaca
    24121 agcgttcggc ttcctcggca tgcggacgga actgctcgcc aataaaatcg gcaatccact
    24181 gggcgcaggc gaggtacaaa tcttctttcg aaccgaagta gtaggtgatg gcagcgatat
    24241 tctgcccggc ctgggcggct atctcgcggg tggtggcgtt cattccatat tcaccaaact
    24301 gcgccagtgc ggcagcaatc agctgttttt tcgcctgttc acccttgatt gtcatggcag
    24361 gattattcat cgcacagtcc attcttaatc aaatgattga ttaagattat gactccatat
    24421 gggagttgtc cagtatggct aagaatttta gctacgccag tcacagggat aatttatgcg
    24481 ctgcgtcaca aaaactgcta cactccgctc cctcatgaca ttgtggtttt tgtcattttc
    24541 cttttcagta tctccctgaa aactacaccg gtaacggtcg gggcggttcg gagtagttat
    24601 gtctttcgat tctttgggtt taagccctga tatcctgcgc gccgttgccg agcagggtta
    24661 ccgtgaaccc acccctattc agcagcaggc gatccctgct gtgctggaag gccgcgacct
    24721 gatggctagc gcccagaccg gcaccggcaa aacagcgggc tttacgctgc cgctgttgca
    24781 acacctgatc actcgccagc cgcacgccaa agggcgtcgt ccggtacgtg cgctcattct
    24841 taccccgacc cgtgaactgg cggcacagat tggcgaaaac gtccgtgatt acagcaaata
    24901 cctgaacatt cgttcgctgg tggtgtttgg cggtgtcagc attaacccac aaatgatgaa
    24961 gttgcgcggc ggcgttgatg tgctggtggc aacgccggga cgtttgctgg acctggaaca
    25021 tcaaaatgca gtgaagctgg atcaggttga aatcctcgtc ctcgatgaag ctgaccgcat
    25081 gctcgacatg ggctttatcc acgatatccg tcgcgtgtta acaaaactgc cagcgaaacg
    25141 ccagaacctg ctgttctctg cgaccttctc tgacgatatt aaagccctgg cggaaaaact
    25201 gttgcacaac ccgctggaaa tcgaagtggc acgccgcaat accgcgtctg atcaggtgac
    25261 ccagcacgtt cactttgtcg ataagaaacg caaacgcgaa ttgctgtcgc acatgattgg
    25321 taaagggaac tggcagcagg tgctggtgtt tacccgtacc aaacacggcg ctaaccatct
    25381 ggctgagcag ctcaataaag atggcatccg tagtgtggcg atccacggga ataaatcgca
    25441 aggtgcgcgt acccgtgcgc tggctgattt taaatcgggt gacattcgcg tactggtggc
    25501 gaccgatatc gctgcgcgcg gtctggatat cgaagagttg ccgcacgtcg tcaactatga
    25561 actgccaaac gtaccggaag attatgtcca ccgtatcggg cgtaccggtc gtgcggctgc
    25621 taccggtgaa gcgttgtcgc tggtgtgtgt tgatgaacac aaactgctgc gtgatatcga
    25681 aaaactgctg aaaaaagaga tcccgcgcat tgcgattccg ggctatgagc cggacccgtc
    25741 aatcaaagcc gaaccgatcc agaacggtcg ccagcaacgt ggcggcatca ggctgatgaa
    25801 caaacgcaaa actgcctgat gcgctacgct tatcaggcct acgtgaactc tgcaatatat
    25861 tgaatttgca tgcttttgta ggccggataa ggcgtttacg ccgcatccgg catttcacaa
    25921 caagcacttg tcagcaattt gagaacacgg gaaaaaattt atctgtttta ccgcccataa
    25981 gccaccgaga cgttacttct ctatagccaa ttgttcgcgc aactccatta aaaggtagcc
    26041 cagtcgattc ttgcccttac cattaccacc gtctccccag taagcatcgt tttccgtatg
    26101 ctcaaccagt tttgcgggcg cggttgccag caagagcaca cgcagttctg catgctgttc
    26161 gaatttagcg cgaagtgctt tacgcatcac ctgttcttta accgactccc agtttttacg
    26221 aagtggcttg gaacgattac gccccatgcg agcggcgacc atgggggaag aaacccgacg
    26281 gatctcttcg cggtattttt catcaaggaa tttttgtgcc tgaaaatagt gttcggaggt
    26341 gggccaggta ttcccgtcaa ccttgatggg cctggcggca aagttagaaa aatcaccgta
    26401 gtcatcgctg gtgctgtaga aatttatgat ggtgtcttgc atgacgtgtt ggatcctttg
    26461 tgctcgaacg ggcattaaac cgcattatgt tggtggttat tgcgagccgc tttccagaaa
    26521 cagaaaaacc attacccctg aaaaccgaaa aatgccacaa tattggctgt ttatacagta
    26581 tttcaggttt tctcatggca ttaaccgccg cgcttaaagc gcaaattgcc gcctggtata
    26641 aggcgcttca ggaacagatc cccgacttta ttccccgtgc gccgcagcgg cagatgattg
    26701 cggacgtcgc caaaacgctg gccggagaag aagggcggca tctggcgatt gaagccccca
    26761 ccggcgttgg gaaaacgctc tcctatttga tccccggcat cgccattgcc cgcgaagagc
    26821 aaaaaacgct ggtggtgagt accgccaacg tggcgttgca ggatcagatt tacagcaaag
    26881 atttaccgct gctgaaaaag atcattcccg atcttaaatt cactgccgct ttcgggcgtg
    26941 ggcgctacgt ttgtccacgt aatctgacgg cgctcgccag cactgaaccc acgcaacagg
    27001 atctgctggc gtttcttgac gacgaactga cgccaaataa tcaggaagag caaaaacgtt
    27061 gtgcgaagct gaagggcgat ctcgacactt ataaatggga tggtctgcgc gatcatacgg
    27121 atatcgctat agatgatgat ctctggcgtc gtttgagtac cgacaaagcc agctgcctca
    27181 accgtaactg ttactactat cgtgaatgcc cgttttttgt tactcgtcgg gagattcagg
    27241 aagcggaagt ggtggtggca aaccatgcgc tggtgatggc ggcgatggaa agcgaagccg
    27301 tactgcctga cccgaaaaat ctgctgctgg tgctggacga aggtcatcac ctgccagatg
    27361 tggcccggga tgcgctggag atgagcgctg aaatcaccgt gccctggtat cgactacagc
    27421 tggacttgtt cacgaaactg gtcgctacct gcatggagca gtttcgcccg aagaccatcc
    27481 caccgctggc gatccctgaa cgtttgaatg cgcattgtga agagttgtat gagcttatcg
    27541 cctcgttaaa caacattctc aatctctaca tgcctgccgg gcaggaggca gagcaccgtt
    27601 ttgcgatggg cgaactgcct gatgaattgc tggagatctg ccagcggctg gcaaaactca
    27661 ccgagatgct gcgtggtctg gcggagttat ttcttaacga tttaagtgag aaaaccggca
    27721 gccacgatat tgtgcgtctg catcggttga ttttacagat gaaccgcgcg ctggggatgt
    27781 ttgaggtgca aagcaaactc tggcggctgg cgtctctggc gcaatcttcc ggtgcgccgg
    27841 tgaccaaatg ggcgacgcgg gaagagcgcg aagggcagct acacctctgg tttcactgcg
    27901 tgggaatacg tgtcagcgat cagctggaaa ggctgctgtg gcgcagtatt ccgcacatta
    27961 ttgtcacctc cgcaaccttg cgttcgctga atagtttttc gcgtttgcag gagatgagtg
    28021 gtctgaaaga gaaagcgggc gaccgttttg tggcgctgga ttcccccttt aaccactgcg
    28081 aacagggcaa aattgttatt ccccggatgc gctttgagcc ttccatcgac aacgaagagc
    28141 aacacattgc cgaaatggcg gcctttttcc gtgaacaggt ggagagcaaa aaatatctcg
    28201 gtatgttggt actgtttgcc agcggacggg cgatgcagcg ctttctcgac tatgtgacgg
    28261 atttacgcct gatgttgctg gtgcagggcg atcagccgcg ttatcgctta gttgaactgc
    28321 accgcaaacg cgttgccaac ggtgagcgta gcgtgctggt gggcttacag tcatttgccg
    28381 aagggcttga tttgaaaggt gatctgctca gccaggtgca tatccacaaa atcgcttttc
    28441 cgcccatcga cagcccggtg gtgatcaccg aaggggaatg gctgaaaagc ctcaaccgct
    28501 atccgtttga ggtgcaaagc ctgccgagcg cctcgtttaa cctgattcag caggttgggc
    28561 gactgattcg aagccacggt tgctggggcg aagtggttat ctacgacaaa cgcttgctga
    28621 caaaaaatta tggcaagcga ctactggatg cattaccggt atttccgata gagcaaccgg
    28681 aagtccctga aggtatagtt aaaaagaaag aaaaaacgaa atccccacgc cgtcggcggc
    28741 gttaatgatg tgagtcaggt aaggagtcgt aaatggacta tcgcaaaatc attaaagaga
    28801 tcgggcgcgg gaaaaaccac gcgcgcgatt tagaccggga tactgcgcgc ggtctgtatg
    28861 ctcatatgct caatggtgaa gtccctgacc ttgagttggg cggcgtattg attgcgctgc
    28921 gtatcaaagg ggaaggggaa gcagagatgc tcggctttta cgaagccatg caaaatcaca
    28981 ccatcaagct gacgccgcct gcaggcaagc cgatgccgat tgtcatcccc agttacaacg
    29041 gcgcgcgtaa acaggctaac ctgacgccat tattggcgat tctgctgcat aaactcggtt
    29101 ttcctgtggt ggttcacgga gttagcgaag atccaactcg cgtgctgaca gaaaccattt
    29161 tcgaactgat gggtattaca ccaacgttgc acggcgggca ggcgcaggcg aagctcgacg
    29221 aacatcaacc ggtgtttatg cctgtcggcg cgttttgccc gccgctggaa aaacaactgg
    29281 cgatgcgctg gcggatgggc gtgcgtaaca gtgcacatac cctggcgaaa ctggcgacgc
    29341 catttgccga aggtgaggca ctgtgtcttt ccagcgtttc gcatccggaa tacattggac
    29401 gcgtcgcgaa gttctttagc gatatcggcg ggtgggcgtt actaatgcat ggtactgaag
    29461 gcgaagtgta tgctaatccg cagcgctgcc cgcagatcaa tctcattgac cgtgaaggga
    29521 tgcgggtgct gtatgaaaaa caggaccctg ctggtagcga gttactgcca caagcaaaag
    29581 atccggaaac cacggcgcag tggactgagc gttgcctggc tggcagcgaa gcgattcccg
    29641 aatcgctgaa aatccagatg gcttgctgcc tggtggctac gggtgaagcg gcaactatca
    29701 gcgatggcct ggcgcgcgtt aatcaggcat tttaattttt atctcagccg gatgagccac
    29761 gtcatccggc ttttacccca ccgcaatatg aaattcctgc atctttattg accttcccgc
    29821 gcccggcgtg cagcataaaa aaacaacaaa cacataacat aaacaggagt taaccatgga
    29881 aagtggtcat cgctttgatg ctcagacgct gcacagtttt attcaggctg tatttcgtca
    29941 gatgggtagc gaggaacaag aagcgaaatt agttgccgat catttaatcg cggcaaacct
    30001 ggcagggcat gattctcatg gtattggcat gatcccaagc tatgtgcgtt cctggagtca
    30061 ggggcatctg caaattaacc atcatgccaa aatcgttaaa gaggcggggg cggcagtcac
    30121 gctcgatggc gatcgcgcat tcggtcaggt cgcggcacac gaagcgatgg cgctggggat
    30181 tgagaaagcg catcagcacg gtattgctgc cgtggcgcta cataactcgc atcatatcgg
    30241 ccgtatcggt tactgggcgg agcagtgttc agcggcgggg tttgtctcta tccactttgt
    30301 tagcgtggtc ggtattccaa tggtcgcgcc gttccacggt cgcgacagcc gctttggcac
    30361 caatcccttc tgtgtggttt tccctcgtaa agataatttc ccgctgttgc ttgattacgc
    30421 caccagcgcc attgcatttg gcaaaacccg cgtcgcctgg cataaaggcg tccccgtgcc
    30481 gccaggttgc ctgattgacg ttaacggcgt gccgacgacc aatccggcgg taatgcagga
    30541 gtcgccgttg gggtcgctgt tgacctttgc cgaacataaa ggctacgccc ttgcagcgat
    30601 gtgtgaaatt cttggcgggg cgctttctgg cggtaaaacg acgcatcagg aaacgttgca
    30661 aaccagcccc gatgccattc ttaactgcat gaccactatc atcatcaacc cggaactgtt
    30721 cggcgcgccg gattgtaacg cgcagaccga agcctttgcc gagtgggtga aagcctcgcc
    30781 gcatgatgac gataagccga ttttgctacc gggcgagtgg gaagtgaaca cgcgtcgcga
    30841 acggcaggag caggggattc cattggatgc gggaagctgg caggccattt gtgatgcggc
    30901 gcggcagatt ggtatgtcgg aagagacgtt gcaggctttc tgtcagcagt tagccagcta
    30961 aaaaaaagcc cgtccagtgg cggacgggca aacaagggta acataggatc aatgagggtt
    31021 agagcatatg cgtctgtcgg caaacagaca gggaaatact tgtgctggac gtagcgtaaa
    31081 cgcctgatcc gtccaacggt tcggattttg taggcctgat aagacgcggt agcgtcgcat
    31141 caggcatctg ttgccggatg cggcataaac gccttatccg tcctacgaat tacttataaa
    31201 ttaccgcagt accgcttaat ttattgttgt tggtggcgga agtgatgctg tatccactgg
    31261 caccggctgc cgccgctttc tcagccagtt tcgcttccag ggcatcgagg gtggatgcgc
    31321 catcggcaga aaccacgccg attttattca tgttctgtgc ctgggatgcc gttaccggtt
    31381 ccgccgcgaa cacgccaaat gacagagttg aaagagccat agcagcaaca acagtattga
    31441 tagttttcat aattaatctc tcgcaggtga tttttgtata agtgacgttg tttcgtcgat
    31501 gtgatgagta tcacgttttt ttacgagaga taaaatcgaa gagaattgac cgccttgttc
    31561 aaataaattg attgatatct aacatactga taaaccattt atttgttgta cttatggact
    31621 tgttttatgc cgcgtggcag tcacagcagc gttaaaggca cggaatgcca cgcaaaatac
    31681 aaattatact cgctatgcat ctcgccagtt gtagttaacg taactgtctg tctttcgaac
    31741 ctctgcgatt atatcctgta tacgctggtt tttgtaaatc tttctcctgc tgacaatgaa
    31801 tacataagcg cacgccagga atggcttccc gacgggcttg cgggatgggg gcaccgcact
    31861 cttcacattc atacaggctt tcgccacgcg gaatttcacc ccgagcgcgg gcaatcgcat
    31921 cttcaattgt actgttgatc tgttcgttga cggcgtcatc gttagcccaa gcggatgcca
    31981 tatcgtcctc cccatatcaa tgcttgtaca gttaagtgta gctaatatag ggacgaacct
    32041 gggcagttca agcgtcagat ctccgaccat tcccgcagca gattatgata aagattgagc
    32101 agcgacagga tctcttcatt ttcaccatag cggcttttca gcgactgaat atttttgtcc
    32161 agttcaaaca gcatggcgcg ctttttatca tcgcggatca tcgactggat ccacataaat
    32221 gatgccactc gtacgccgcg ggttacgggt gtcacgcaat gcaggctgct ggagggatac
    32281 aacaccagat cacctgccgg gagttttacc cgatgttgtc caaaggtgtc attaacgacc
    32341 agttcgccgc cgtcgtagct ttgtggatcg cttaaaaaca gcgtggcaga aaggtcagtg
    32401 cgcatccagc cgttttgtgg atggctgcgt actgcgccat ccacatggaa accataggtt
    32461 tcattgttct gatagcgatt aaacagcggc gtggaaaggg tacgcggcaa ggcagcggca
    32521 aagaacaatg catgttggtt aaccgcgttc agcacctcat tttgcagtgc ggcgtataac
    32581 gcgctgcggg tgtctacttg ctgattgttc ttaacttgtg caccttgtgc tccggtggtg
    32641 acgcgtccat ccacccattc ggcttgttcc agttgttcgc gaaaatgggc gacgtcctgt
    32701 ggcgataaca cgccgggaat gtggtacatc atacaagttc tccaaaaagt ggggcctgcg
    32761 ccccacatct gaatcagaaa tgcatattgg ctgtgagcaa gaaggttctt ggctcgcccg
    32821 ggtgataacg gtagccgctc ttgttgattg aggcgacgta atcggtatca aacaggttgt
    32881 aaacgtttag ctggaagtcg agattgcgat taacccgata ccccagtttg gcatcggcga
    32941 cccagtaacc ttctgtaaat gctggcgttc ccaccgcgcc gtctgaacct ttatgtatac
    33001 tgccgatata gcgtgcgccc gcgccaacag agatatcatc ggttgcctga tattggctcc
    33061 ataaggtgaa ggcgtgctcc ggggtatacg gcagcgatga ggaaccatcc tgggcaacat
    33121 ctttgccgtt tttgatggtt gctttttgct gggtatagcc gccaatcacc tgccacgcgg
    33181 gagtgatatt ccctgccacg gatatctcat agccttcgac gcgtttctta ccgtattgcg
    33241 agtaagtccc gtcatcattt tgctcaactt cattttcgat atcagtacgg aacagcgcgg
    33301 cggtgagcaa caggcgttta tccagaacct gccatttggt gccaatctcg ctggtgttgg
    33361 ctttttgcgg tttaaaatcg gtgcggttgg cactgttacc actgccagac tgcgcaaggg
    33421 cgaagttgtt gccgcccgga ggctgctggg aaacggcata gttaatatag acattgccgt
    33481 tttccgtcag gtggtacagc gccccggctt tccagttcac cagattgccc gacttggcgg
    33541 tgtcgacggt ggtgactgga gaaccttttg ccacaccagc tgggcaggtg atggcaccgc
    33601 gtccgctgcc gccgcaggcg gtggcactgt catattcagt atgataatta tccagacgga
    33661 tcccgccgtt cagctcaaaa tcacgggtga tttgcagcgt atcgaaggcg taaattgcga
    33721 aggtatccgt ctgaccattg gcgtttgcgc cgttgcgcgt caggccgccg ggatgaattc
    33781 tgctgtcagg atgataaata tttaccgccg gtaacgtcac cggattaacg ccatagttag
    33841 tctgcgtttc acgggtaaat tccacgccgg tactgacatc atgaccgata gaaccggtat
    33901 agaacgtcga ggtcaggttg gtctggttgg tgagaatttt attactcaca tctttggtat
    33961 tcgccgtgcg tgactaggtc cagctattca catcgctggt gggctgagta atattcgacg
    34021 ccccgcccat aatcgccgtc atcaggtaat cctgctttac gcgcgaccaa cgggtagtat
    34081 tgcgaatggt ggtgttatcg ttgatgtcgt gctcaaaacg catggtggcg gtgtcggtgg
    34141 tcgaatcgtc gtaatcggaa tccgtgccgt aaaagttatg agtatcaact tttccggaat
    34201 gattcagggc cgccgctccc gcagatgggg cagaatagcc cggcaaaccg atggtcggaa
    34261 tgccgccgtc tggcgtgttg tgctgagtga catgcagata attaagatac aaacgatttg
    34321 ctgtaccaag gccaaaagcg acagaagggg cgacgccgta acgctcattt ttgactttgt
    34381 cgcgtccggc atcgtgggtt ttttcgccca tcacattcag gcgcaccgcg gtagtatcac
    34441 caatgacctg attgacgtcc agcgtgccgc ggcggaacca ggcgctgcca atactggcgg
    34501 aggcgtcaat gccggaatca ttgcgcggct gcttgctgat catattgatc gagcctgtcg
    34561 gtgcgctgcg cccgtagtcg gtgccggacg gccctttaat cacttcgacc tgctcggtat
    34621 tgaaggtgtc gcgcgagacg ctgccgatat cgcgaatacc atcaatataa atactgttag
    34681 aggtatcggc accacgcata taaatggcgt cgccagtggt ggagttaccg ttctcacccg
    34741 caaaaaacgc acccacgccg g
//
LOCUS       DAJTRD010000036        34447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTRD010000036 DAJTRD010000000
VERSION     DAJTRD010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.036"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..334)
                     /locus_tag="OO315_001912"
     CDS             complement(<1..334)
                     /locus_tag="OO315_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890837.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            704..1654
                     /gene="corA"
                     /locus_tag="OO315_001913"
     CDS             704..1654
                     /gene="corA"
                     /locus_tag="OO315_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5890838.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1695..2075)
                     /locus_tag="OO315_001914"
     CDS             complement(1695..2075)
                     /locus_tag="OO315_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5890839.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2089..2505)
                     /locus_tag="OO315_001915"
     CDS             complement(2089..2505)
                     /locus_tag="OO315_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890840.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="OO315_001916"
     CDS             complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="OO315_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5890841.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3506..3973)
                     /locus_tag="OO315_001917"
     CDS             complement(3506..3973)
                     /locus_tag="OO315_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5890842.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4138..5007
                     /gene="pldA"
                     /locus_tag="OO315_001918"
     CDS             4138..5007
                     /gene="pldA"
                     /locus_tag="OO315_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5890843.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5140..6969
                     /gene="recQ"
                     /locus_tag="OO315_001919"
     CDS             5140..6969
                     /gene="recQ"
                     /locus_tag="OO315_001919"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5890844.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7033..7653
                     /gene="rhtC"
                     /locus_tag="OO315_001920"
     CDS             7033..7653
                     /gene="rhtC"
                     /locus_tag="OO315_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5890845.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="OO315_001921"
     CDS             complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="OO315_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5890846.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8446..9468
                     /gene="pldB"
                     /locus_tag="OO315_001922"
     CDS             8446..9468
                     /gene="pldB"
                     /locus_tag="OO315_001922"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5890847.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9476..10276
                     /gene="yigL"
                     /locus_tag="OO315_001923"
     CDS             9476..10276
                     /gene="yigL"
                     /locus_tag="OO315_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5890848.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10352..11251
                     /gene="bioP"
                     /locus_tag="OO315_001924"
     CDS             10352..11251
                     /gene="bioP"
                     /locus_tag="OO315_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5890849.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11139..12092)
                     /gene="metR"
                     /locus_tag="OO315_001925"
     CDS             complement(11139..12092)
                     /gene="metR"
                     /locus_tag="OO315_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5890850.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12328..14589
                     /gene="metE"
                     /locus_tag="OO315_001926"
     CDS             12328..14589
                     /gene="metE"
                     /locus_tag="OO315_001926"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5890851.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14628..15443)
                     /locus_tag="OO315_001927"
     CDS             complement(14628..15443)
                     /locus_tag="OO315_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5890852.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15705..16466
                     /gene="udp"
                     /locus_tag="OO315_001928"
     CDS             15705..16466
                     /gene="udp"
                     /locus_tag="OO315_001928"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5890853.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16607..18034
                     /gene="rmuC"
                     /locus_tag="OO315_001929"
     CDS             16607..18034
                     /gene="rmuC"
                     /locus_tag="OO315_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5890854.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18129..18884
                     /gene="ubiE"
                     /locus_tag="OO315_001930"
     CDS             18129..18884
                     /gene="ubiE"
                     /locus_tag="OO315_001930"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5890855.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18898..19503
                     /gene="ubiJ"
                     /locus_tag="OO315_001931"
     CDS             18898..19503
                     /gene="ubiJ"
                     /locus_tag="OO315_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5890856.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19500..21140
                     /gene="ubiB"
                     /locus_tag="OO315_001932"
     CDS             19500..21140
                     /gene="ubiB"
                     /locus_tag="OO315_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5890857.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21219..21488
                     /gene="tatA"
                     /locus_tag="OO315_001933"
     CDS             21219..21488
                     /gene="tatA"
                     /locus_tag="OO315_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5890858.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21492..22007
                     /gene="tatB"
                     /locus_tag="OO315_001934"
     CDS             21492..22007
                     /gene="tatB"
                     /locus_tag="OO315_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5890859.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22010..22786
                     /gene="tatC"
                     /locus_tag="OO315_001935"
     CDS             22010..22786
                     /gene="tatC"
                     /locus_tag="OO315_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5890860.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22828..23610
                     /gene="tatD"
                     /locus_tag="OO315_001936"
     CDS             22828..23610
                     /gene="tatD"
                     /locus_tag="OO315_001936"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5890861.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23607..24095)
                     /gene="rfaH"
                     /locus_tag="OO315_001937"
     CDS             complement(23607..24095)
                     /gene="rfaH"
                     /locus_tag="OO315_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5890862.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24262..25755
                     /gene="ubiD"
                     /locus_tag="OO315_001938"
     CDS             24262..25755
                     /gene="ubiD"
                     /locus_tag="OO315_001938"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5890863.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25801..26502
                     /gene="fre"
                     /locus_tag="OO315_001939"
     CDS             25801..26502
                     /gene="fre"
                     /locus_tag="OO315_001939"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5890864.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26694..27857)
                     /gene="fadA"
                     /locus_tag="OO315_001940"
     CDS             complement(26694..27857)
                     /gene="fadA"
                     /locus_tag="OO315_001940"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5890865.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27867..30056)
                     /gene="fadB"
                     /locus_tag="OO315_001941"
     CDS             complement(27867..30056)
                     /gene="fadB"
                     /locus_tag="OO315_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5890866.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30246..31577
                     /gene="pepQ"
                     /locus_tag="OO315_001942"
     CDS             30246..31577
                     /gene="pepQ"
                     /locus_tag="OO315_001942"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5890867.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31577..32191
                     /locus_tag="OO315_001943"
     CDS             31577..32191
                     /locus_tag="OO315_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5890868.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32230..33681
                     /gene="trkH"
                     /locus_tag="OO315_001944"
     CDS             32230..33681
                     /gene="trkH"
                     /locus_tag="OO315_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5890869.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33693..34238
                     /gene="hemG"
                     /locus_tag="OO315_001945"
     CDS             33693..34238
                     /gene="hemG"
                     /locus_tag="OO315_001945"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5890870.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8701 c         9302 g         8336 t
ORIGIN      
        1 ccttctgctg accgttttcg atgtacaaac cgagcggcgc atagctttca tcatagatgc
       61 cgccgttcat cgccatctgc acctgaccct gactattaat atccgccagc agagcatgta
      121 acgttcccca cgcttcgcca ttggcttttt gccagtacat tttcacccgc tctgtttgag
      181 gattaacggt atacgcctgt acggtcagcg tcggatctga gagtgcgcaa tcatcagcgg
      241 caacagcaaa caagggaagt aaggtgaggg tgagaaaaat ccgtttgaga ttcaaggtga
      301 tcattccttt accaatgagt agccgatgcg ccattatagg tcctagatgt gggatttttt
      361 tatcctgtta gcgaccttga cgagtaccaa aaagcgcgaa gttcaactat tgttctgtgg
      421 tgttctgttg cgtgttgacg gcaaaatttt gctggcgtaa catgcgcgca cgatcactct
      481 aagaggacat tcgccttgga cacacccagt agatactggc tcactatcct gtcatccagg
      541 atcaactcct aaggctatcc ctttttgctg atagccttag cggttgtcag cgacctcaat
      601 ttttcccgtc gcgctgagtc aggctgttta atggtctgaa acccaatttg tttctgtgtg
      661 cccaccgaac tgtccgatat tttaagcatt gggagtcccg gtcatgctga gcgcatttca
      721 actggaaaat aaccgactga cccggctgga agtcgaagag tcacaacccc ttgtaaatgc
      781 agtatggatt gatcttgtcg aaccggacga cgacgagcga ctgcgcgtac aatctgaact
      841 tggccagagc ctggcaaccc gcccggaact ggaagacatc gaagcatcgg cacgtttctt
      901 tgaagacgac gacggcctgc atattcactc cttcttcttc tttgaagatg cggaagatca
      961 cgccggtaac tccactgtgg catttaccat ccgtgatggt cgtctgttta ctctgcgtga
     1021 gcgtgaactg cccgcttttc gtctgtatcg tatgcgtgcc cgtagccagt cgatggtaga
     1081 cggtaacgcc tacgagttgc tgctggatct gttcgaaacc aaaatcgaac agttggcaga
     1141 tgaaattgaa aatatctata gcgacctgga gcagttgagc cgggtgatta tggaagggca
     1201 tcagggcgat gagtacgacg aggcgctctc cacgctggcg gaactggaag atatcggctg
     1261 gaaagttcgc ctgtgtctga tggataccca gcgcgctctc aacttcctgg tgcgtaaagc
     1321 gcgtttaccg ggtgggcaac tggagcaggc gcgtgaaatc ctgcgcgata tcgaatccct
     1381 gctgccgcat aacgaatccc tgttccagaa ggtgaacttc ctgatgcagg cggcaatggg
     1441 ttttatcaac atcgagcaga accgcatcat caaaattttc tcggtggtat ccgtcgtgtt
     1501 cctgccgcca acgctggttg cttccagcta cgggatgaac tttgagttta tgccagaact
     1561 gaagtggagt ttcggctacc ctggcgcgat tatctttatg atcctcgcgg gcctggcacc
     1621 gtatctgtac tttaagcgga agaactggtt gtaaaaagaa ggcggtggct tagcctggct
     1681 aagccacctg ttattcaaag gctccaggta tttaaccctt ttacctcttt ctcataaaac
     1741 catttgttcg tgttaacagc aacataggct gctacggcaa ttcccagaat gttaacgcca
     1801 attaatgcac caacaaatag agatagaatg ccaagtaata gaactataat tgcttttttc
     1861 cataagccca gaacaaataa atagatccag gaacagaaga aagcaatgaa attcatttga
     1921 atagttaatc gttgtcttac ttttaatgct ttaaatgctg ctttatattc tggtgttgct
     1981 ccccagaatc caggaaagcc atgttgatca taaaaattaa atcggtattt ccatttttca
     2041 cttaatgaat catcgttcat atattcctta ctcataaata ctccataact attgttttga
     2101 tgaatcagta ggtgcaagca ttagcatact gaaagtggaa aaataacaaa tcaaaaaaat
     2161 catcgaacca ttgcctgaac aggcaaaatc ttcggctatc attgtgatga tagagatgat
     2221 atatactgct aatgtaccaa aaacataaat ttttatatat atgaaaccac tatcacggag
     2281 tcgttggcaa ttcatgttga tgacgagata atggagtgcg atggtagaga ctataacaag
     2341 aaaacctgct tccccatcgt taaaaaagat aataagaaag gcaaaaatga aatttattaa
     2401 aataaataaa aatatatatc ggtgtctgga aatcttaccg ttagatgttg ggataaacat
     2461 acgtaacata aattttacat ccttgtgtga gtctcctgtc agcatggcaa tatgcccact
     2521 ctcatacaag agtcggcata ttttttcaga atatatttat tttttatttg gacgttctac
     2581 gctgcgtata aatcgcgtcc atcacaaaaa ttgccagcgc cacccaaata aaggtgaaag
     2641 tcaccatctt atcggcaccc ggtttttcac cataaaacgt cacagccagc aggaacatca
     2701 gcgtcgggcc aatgtactga aaaaagccta acgttgagag acgcaggcgc gtggcagcgg
     2761 cagtaaaaca caacagcggt acggtagtga caataccggc ggcgatcagc agtaaattca
     2821 gcgacatcgg gttttgcccc atatggctgg ttgaactgtc ggcaatagca aacaggtaaa
     2881 tcgccgccac gggcagcagc cacatggttt caattaacat gccggtttgt gcttctaccg
     2941 caatcttctt gcgcaccagg ccgtagaagg caaaactaaa tgccagcccc agcgcgataa
     3001 taggtagcga accaaaagtc cacagctgga ccaacacgcc acatatcgcc agaatcaccg
     3061 ccagccattg catccggcgg aatcgctcgc cgaggaaaat catccccagc acaatgttca
     3121 ccagcgggtt aataaagtaa ccaaggctcg cttccagcat atggtgattg ttcaccgccc
     3181 agataaacag tagccagttg ccaccaatca gtacggcaga gactgccagc ataaaaattt
     3241 tctgtggcgt ctgaatcagc gtttttaaat aggaccactg gcggcaaatg ctcatcagca
     3301 ccaccataaa gaaaaacgac cagatcacgc gatgcgtcag gatctcatcg gcgggcacgt
     3361 agtaaatcag cttgaagtat gctggcgcaa taccccaaat aaaataagcg gcaagagcga
     3421 gtaatacgcc ctgccgcgtt tgttttgcat ccatcgggaa tactcatttt taattggtaa
     3481 cagcagttta cctgctttta tgtcttcaac ctaccatata ggtggcggtg gcactggcaa
     3541 tataaagctg ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta ttgcctgcac
     3601 gcaacaggct actggtagca gtaaaacgct cgcccctgcc tgggcgcaga taatcaacgc
     3661 gaagatcaat ggtccccatc cgcgatagcc gctggcgtag ttcatcttca ctgatggttt
     3721 cgtggcgggt taaggtactt cccacgcaca ccagaccggc ggcgacatcc agcgccgacg
     3781 caatgactcc gccgtgcaaa atgctttgcg cccagttacc caccatcatc ggctgatttt
     3841 taaaggccag ctgtgcgaac tccttttcgt aacgctccag ttccatcccc aatgcgcggt
     3901 taaatggcat gtgataaaca aacatctcac ccactaattt cagggcttgt tcagcggtca
     3961 gtacggcaga catatcatcc ttacacttca ttggttaatg aaatgttgat tttatgcttc
     4021 tttgttgttg gtttctactt taggaaggga taactaacgg caatggagtt aagtatgtaa
     4081 aatagcccgc agaaaaacat tcaccttatc aataattcgt tacggagaac acgaccgatg
     4141 cggactctgc agggctggtt gttgccggtg tttatgttgc ctatggcagt atatgcacaa
     4201 gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat tatcgccaat
     4261 atgctgcagg agcatgacaa tccgttcacg ctctatcctt atgacaccaa ctacctcatc
     4321 tacacccaaa ccagcgatct gaataaagaa gcgattgcca gttacgactg ggcggaaaat
     4381 gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat ttccgctgtg gcgtgggatt
     4441 ttaggcccaa actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg gcaactgtcc
     4501 aatagcgaag agtcttcacc gtttcgtgaa accaactacg aaccgcaatt gttcctcggt
     4561 tttgccaccg attaccgttt tgcaggttgg acgctgcgcg atgtggagat ggggtataac
     4621 cacgactcta acgggcgttc agacccgacc tcccgcagct ggaaccgcct ttatactcgc
     4681 ctgatggcag aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt ggtgggtaat
     4741 actgacgata acccggatat caccaaatat atgggttact accagcttaa aatcggctat
     4801 cacctcggtg atgcggtgct cagtgcgaaa ggacagtaca actggaacac cggctacggc
     4861 ggcgcggagt taggcttaag ttacccgatc accaaacatg tgcgccttta tactcaggtt
     4921 tacagcggct atggcgaatc gctcatcgac tataacttca accagacccg tgtcggtgta
     4981 ggggttatgc taaacgactt gttttgatga acggttgagt ggttggcaaa tctggaatcc
     5041 agcatccagg attaccctct cagagactaa aagcattgca gtttctcgcg caggcgctga
     5101 aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc ggaagtgttg
     5161 aatctggagt ccggagctaa acaggtttta caagaaacct ttggctacca acagtttcgc
     5221 cccggccagg aagaaattat cgacactgtg ctttccggtc gcgattgcct ggttgttatg
     5281 cccactggtg gcggaaaatc cctttgctat caaattcctg ccttattgct aaacggcctt
     5341 accgtggttg tttcaccgct gatttcgttg atgaaagatc aggtggatca actacaggcc
     5401 aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc gcgaacagca acttgaagtg
     5461 atgacaggct gccgcaccgg gcaaattcgc ttactgtata tcgccccgga acgcctgatg
     5521 ctggataact ttcttgagca tctggcgcac tggaatccgg tgttattagc cgtcgatgaa
     5581 gcgcactgta tctcccaatg gggtcacgat ttccgcccgg aatatgccgc gctcggtcag
     5641 ttgcgccagc ggttcccgac gctgccgttt atggcgctga ccgccacagc cgacgacacc
     5701 acgcgccagg atatcgtgcg cctgctgggg ctgaacgatc cgctgattca aatcagcagt
     5761 tttgaccgtc cgaatattcg ctacatgctg atggagaaat tcaaaccgct cgatcagttg
     5821 atgcgctacg tgcaggaaca gcgcggtaag tctggcatta tctactgcaa cagccgggcg
     5881 aaagtagaag acaccgctgc gcgcctgcaa agcaggggta ttagcgcggc ggcctatcat
     5941 gccgggctgg aaaataatgt ccgcgccgac gtgcaggaga aattccagcg cgatgatctg
     6001 caaattgtgg tggcgacggt ggcgttcggc atgggcatca ataaaccaaa cgttcgcttc
     6061 gtggtccact ttgatattcc gcgcaatatc gaatcctatt atcaggaaac cggtcgcgcc
     6121 gggcgtgatg gcctgcctgc ggaagcgatg ctgttttacg atccggctga tatggcgtgg
     6181 ctgcgccgtt gtctggaaga gaagccgcag gggcagttgc aggatatcga gcgccacaaa
     6241 ctcaatgcga tgggcgcgtt tgccgaagcg caaacttgcc gtcgtctggt attgctgaac
     6301 tattttggcg aagggcgtca ggagccgtgc gggaactgcg atatctgcct cgatccgccg
     6361 aaacagtacg acggttcaac cgatgctcag attgcccttt ccaccattgg tcgtgtgaat
     6421 cagcggtttg ggatgggtta tgtggtggaa gtgattcgtg gtgctaataa ccagcgtatc
     6481 cgcgactatg gtcatgacaa actgaaagtc tatggtatgg gccgtgataa aagccatgaa
     6541 cattgggtga gcgtgatccg ccagctgatt catctcggcc tggtgacgca aaatattgcc
     6601 cagcattctg ccctacaact gacagaggcc gcgcgcccgg tgctgcgcgg cgaatcctct
     6661 ttgcaacttg ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca gaaatcgttc
     6721 ggcggcaact atgatcgcaa actgttcgcc aaattacgca aactgcgtaa atcgattgcc
     6781 gatgaaagta atgtcccgcc gtacgtggtg tttaacgacg caaccttgat tgagatggct
     6841 gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg gatgcgcaag
     6901 ctggaacgct ttggtaaacc gtttatggcg ctgattcgtg cgcatgtcga tggcgacgac
     6961 gaagagtagt cagcagcata aaaaagtgcc agtatgaaga ctccgtaaac gtttcccccg
     7021 cgagtcaaat gtatgttgat gttatttctc accgtcgcca tggtgcacat tgtggcgctt
     7081 atgagccccg gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg ttcccgtaaa
     7141 gaagcgatga tgggcgtgct gggcattacc tgcggcgtaa tggtttgggc tgggattgcg
     7201 ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc tgcatacgct gattatggta
     7261 ggcggtggcc tgtatctctg ctggatgggt taccagatgc tacgcggtgc actgaaaaaa
     7321 gaggctgtat ctgcgcctgc accacaggtt gagctggcga aaagcgggcg cagtttcctg
     7381 aaaggcttac tgaccaatct cgccaatccg aaagcgatta tctattttgg ttcggtattc
     7441 tcattgtttg tcggcgataa cgttggcact accgagcgct ggggcatttt cgcgttgatt
     7501 attgtcgaaa cactggcgtg gtttaccgtc gttgccagtc tgtttgccct gccgcaaatg
     7561 cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt ttgccggggc gttatttgcc
     7621 ggatttggca ttcatttgat tatttcgcgg tgatgccaga cgcgtcttca gagtaagtcg
     7681 gataaggcgt ttacgccgca tccgacatta tttttcacgc atgcctcgcc gatgctaaca
     7741 gcgctcccac cagcataaac aacgagccga aaatcttatt cagcgccttc atctgctttg
     7801 gtcctttaat ccatagagca atcctttgag caagggtggc gtaaccgatc atcacaataa
     7861 tatcgaccac aatagtggtg acgccgagca cgatatactg catcagttgc ggctgttgcg
     7921 gcatgatgaa ttgcggaaat agcgccgcca gaaacacaat acttttggga ttggtgagat
     7981 tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg agtagaggcc agcgatttaa
     8041 gatcaattgc gccagcggcg cgccactgct ggattcccag ccaaatcaag taagccgcgc
     8101 ctgcccactt taacacttca aacgcaatca ctgagcggga aaatagcgtc cccaacccca
     8161 cgccaaccag cacaatatga atcgccagtc cggtctgaag cccagcaata gacgccaccg
     8221 cgccgcgata accgtggttg agcgaggtgg tcatagtgtt gattgcacca gagcctggcg
     8281 acaggcttaa aatgatcgat gtcagcaggt aggcaaacca ccattctaag gtcatgatga
     8341 actcccggtg tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt gtgtcaggca
     8401 cgcttaaaaa aacgatttta cgtggtttaa gaggcagatt acccgatgtt tcagcagcaa
     8461 aaagactggg aaacaagaga aaacgcgttt gctgctttta ccatgggacc gctgactgat
     8521 ttctggcgtc agcgtgatga agcagagttt actggtgtgg atgacattcc ggtgcgcttt
     8581 gtccgttttc gcgcacagca ccatgaccgg gtggtagtca tctgcccggg gcgtattgag
     8641 agctacgtaa aatatgcgga actggcctac gacctgttcc atttggggtt tgatgtctta
     8701 atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc gcatctcggg
     8761 catgttaatc gctttaatga ttatgttgat gatctggcgg cattctggca gcaggaggtt
     8821 cagcccggtc cgtggcgtaa acgctatata ctggcacatt cgatgggcgg tgcgatctcc
     8881 acattatttc tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac tgcgccaatg
     8941 tttgggatcg tgattcgtat gccgtcattt atggcacggc agatcctcaa ctgggccgaa
     9001 gcgcatccac gtttccgtga tggctatgca ataggcaccg ggcgctggcg cgcgttgccg
     9061 tttgctatca acgtactgac ccacagcaga cagcgatatc gacgtaactt acgcttctat
     9121 gctgatgacc caacgattcg cgtcggtggg ccgacctacc attgggtacg cgaaagtatt
     9181 ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg ccacgcaaac gcttctctta
     9241 caagctgaag aggaacgcgt ggtggataac cgcatgcacg accgtttttg tgaactccgc
     9301 accgccgcgg gccatcctgt cgaaggagga cggccgttgg taataaaagg tgcttaccat
     9361 gagatccttt ttgaaaagga cgcaatgcgc tcagtcgcgc tccacgccat cgttgatttt
     9421 ttcaacaggc ataactcacc cagcggaaac cgctctacag aggtttaaat ttcttatgta
     9481 ccaggttgtt gcgtctgatt tagatggcac gttactttct cccgaccata cgttatcccc
     9541 ttacgccaaa gaaactctga agctgctcac cgcgcgcggc atcaactttg tgtttgcgac
     9601 cggtcgtcac cacgttgatg tggggcaaat tcgcgataat ctggagatta agtcttacat
     9661 gattacctcc aatggtgcgc gcgttcacga tctggatggc aatctgattt ttgctcataa
     9721 cctggatcgc gacattgcca gcgatctgtt tggcgtagtc aacgacaatc cggacatcat
     9781 tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg aagagatgcg
     9841 cttttttaaa gaagcggtgt tcaaatatgc gctgtatgag ccgggattac tggagccgga
     9901 aggcatcagc aaagtgtttt tcacctgcga ttcccatgaa caactgctgc cgctggagca
     9961 ggcgattaac gctcgctggg gcgatcgcgt caacgtcagt ttctctacct taacctgtct
    10021 ggaagtgatg gcgggcggcg tgtcgaaagg ccatgcgctg gaagcagtgg cgaagaaact
    10081 gggctacagc ctgaaggatt gtattgcgtt tggtgacggg atgaacgacg ccgaaatgct
    10141 gtcgatggcg gggaaaggct gcattatggg cagtgcgcac cagcgtctga aagatctgca
    10201 tccggagctg gaagttattg gtactaatgc cgacgacgcg gtgccgcatt atctgcgtaa
    10261 actctattta tcgtaatcgt tctttatttg gtcagttgtc aacctgatac ttcgctacaa
    10321 tggatactcg ttaatcaaag agttttccat tgtggcgtta cttatcatca ccacgattct
    10381 gtgggccttc tcctttagct tttatggcga gtaccttgcg gggcacgtcg atagctattt
    10441 tgcggtgctg gtgcgcgttg gcttggcggc actcgttttt ctgccgtttc tgcgtacccg
    10501 tagcaatagc ctgaaaacgg tcggcctgta tatgctggtg ggcgcgatgc agctcggcgt
    10561 gatgtatatg ctgagtttcc gcgcttatct ctacctgacg gtctccgaat tgttgctgtt
    10621 caccgtactg acgccgctct acatcacgct gatttatgac atcatgagta agcgccgtct
    10681 gcgctggggc tatgctttta gcgccttgct ggcggtgatt ggtgccggga ttattcgcta
    10741 tgatcaggtc accgaccatt tctggactgg cttgttgctg gtgcaactct ccaatatcac
    10801 ttttgccatc ggcatggtgg gttacaaacg cctgatggaa actcgcccga tgccgcagta
    10861 taacgccttt gcgtggttct atcttggcgc gttcctggtg gcggtaattg catggttctt
    10921 gctgggaaat gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc tggtgtttct
    10981 tggcgtggtg gcttccggga ttggctactt tatgtggaac tacggcgcga cgcaggtgga
    11041 cgccggaacg ctgggcatta tgaataatat gcacgttccg gcagggctgc tggtaaacct
    11101 ggctatctgg caccaacagc cgcactggcc aacgtttatt acaggcgcgc tggtgatcct
    11161 ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa cggcagatga
    11221 tcgcaggcgt gattgcgcgc tgagcgaata aacgcttccg taactggctg acgctgctcg
    11281 ccatcgcgca cggcggcgta cagtcggctc cacaagcctt cgcccagggt tttggtcacc
    11341 accagaccct ggcgctcaaa actctctact acccaatgcg gtagcgcggc aatacccatc
    11401 cgcgcggcaa ccatttgaat caacaataag gtgttatcga cgcttttcag tgacgggctg
    11461 acgcctgccg gctgaagaaa atgccgccag acatccagtc gactacgctg caccggataa
    11521 attaatagcg tctcgctggc gagatcttcc ggtgtaatac gcgtttttgc cgccagtgga
    11581 tggtcaggtg ctaacaccag gcgtacttca taatcgaaca tcggcgaata atgcaggcca
    11641 ctgcgcggca ggatatcgga ggtcatcacc agatccagct cgccctgttg caacgcgggc
    11701 tgcgggtcaa acgtcacgcc cgatttaaaa tccatctcta cctgcggcca gttcttatgg
    11761 aaatttccta aagcgggtgt cagccactga atacagctat ggcactcaat ggcaatgcgc
    11821 agacgcgtct gctgcggttc attgcaggct tgcagtgcct ggctgatttg cggcagcacc
    11881 tggtttgcca gttgcaacag gatttctccc tgcggtgtaa agcgtagcgg ctggctctta
    11941 cgcacaaata gccggaagcc aaggcgttgt tccagatcgc taaactggtg agacagggcg
    12001 gattgcgtct gatgcaacgt tgccgcagcg gctgcgagcg agccgcagtt ccgcaacgct
    12061 tgtagcgttt tcaggtgttt tacttcgatc atgaaagtcc ttcacttcgg catgaataat
    12121 ttgcgcttga ggaatataca gtaaccgcca attatggatg tgtaaacatc tggacggcta
    12181 aaatcattcg tcttttaaat ttatggtgcg ttggctgcgt ttctccaccc cggtcactta
    12241 cttcagtaag ctcccggcga tgaataaact tgccgccttc cctaaattca aaatccatag
    12301 gatttacata taattagagg aagaaaaatg acaatattga atcacaccct cggtttccct
    12361 cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa gttattgggc ggggaactcc
    12421 acgcgtgaag aactgctgac agtagggcgt gaactgcgtg ctcgtcactg ggatcaacaa
    12481 aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga tcatgtactg
    12541 accaccagtt tgctgctggg taatgttccg ccacgtcatc agaacaaaga cggttcggta
    12601 gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga acctgcggcg
    12661 gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc ggagttcgtt
    12721 aaaggccaac agttcaaact gacttggacg cagctgctgg aggaagtgga cgaggcgctg
    12781 gcgctgagcc acaacgttaa gcctgtgctg ctggggccgg ttacttacct gtggctgggg
    12841 aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga acgacattct gccggtttat
    12901 cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt gggtacagat tgatgaaccc
    12961 gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg
    13021 ctccagggac aggtgaaact gctgctgacc acctattttg aaggcgtaac gccaaatctc
    13081 gacacgatta ctgcgctgcc tgttcagggg ctgcatgtgg accttgtaca tggtaaagat
    13141 aacgttgttg aactgcacaa gcgcctgcct tctgactggc tgctgtctgc gggtctgatc
    13201 aatggtcgta acgtctggcg cgccgatctt actgagaaat atgcgcaaat taaggacatt
    13261 gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag ccccatcgac
    13321 ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt cgccctacaa
    13381 aaatgccatg aactggcact gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg
    13441 gcagagtgga gcgccccaat tcaggcgcgt cgtcactcta ctcgtgtaca taatccggcg
    13501 gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa tgtctatgaa
    13561 gtgcgtgctg aagcccagcg tgcgcgtttt aaactgccag cgtggccgac caccacgatt
    13621 ggttccttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa aaagggcaat
    13681 ctcgacgcca acaactaccg cacgggcatt gcggaacata tcaagcaggc cattgttgag
    13741 caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa tgacatggtg
    13801 gaatactttg gcgagcacct cgacggattt gtctttacgc aaaacggttg ggtacagagc
    13861 tacggttccc gctgcgtgaa gccaccgatt gtcattggtg acattagccg cccggcaccg
    13921 attaccgtgg agtgggcgaa gtatgcgcaa tcgctgaccg acaaaccggt gaaagggatg
    13981 ctgacggggc cggtgaccat actctgctgg tcgttcccgc gtgaagatgt cagccgtgaa
    14041 accatcgcca aacagattgc gctggcgctg cgtgatgaag tggccgatct ggaagccgct
    14101 ggaattggca tcatccagat tgacgaaccg gcgctgcgcg aaggtttacc gctgcgtcgt
    14161 agcgactggg atgcgtatct ccagtggggc gtagaggcct tccgtatcaa cgccgccgtg
    14221 gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa cgacatcatg
    14281 gattcgattg cggcgctgga cgcagacgtc atcaccatcg aaacctcgcg ttccgacatg
    14341 gagttgctgg agtcgtttga agagtttgat tatccaaatg aaatcggtcc tggcgtctat
    14401 gacattcact cgccaaacgt accgagcgtg gaatggattg aagccttgct gaagaaagcg
    14461 gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccgg actgtggcct gaaaacgcgc
    14521 ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca gaatttgcgt
    14581 cgggggtaaa tccaaaccgg gtggtaatac cacccggtct tttctcatta cagcgacttc
    14641 ttcccaccat actgcttaaa ccattccagc atacgctgcc agccatcttt cgcagattcg
    14701 gcatgatagc tcgggcgata atcagcgttg aatgcatgcc cggcatccgg gtacacgata
    14761 atctctgctt tcgcattagc agcccgcagc gcctggcgca tcgtttcaac gctctcctgc
    14821 ggaatgctgt tatcctgacc accatataag ccgagaaccg gcgcgttaag atcggttgcg
    14881 atatcaacag gttgtttcgg tgaattcagc gacttgtcgc ccgtcagttt gccgtaccac
    14941 gccactgcgg cttttagctg tggattatgc gcggcataca gccaggtgat acgtccaccc
    15001 cagcagaatc cggtgatcat taaacgatga acatcgccgc cgttgcggga agcccaactg
    15061 gcgacatgat cgagatcggc cagcacctgc gagtcaggca cttttgctac cagaccgcta
    15121 agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt cgcggaagta aagttcaggt
    15181 gcgatagcca gatacccctc cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc
    15241 acgccaaaaa tttcctgcac tacaatgacc actggcagtg ggccatcgct ttgctttggt
    15301 ctggcatggt aagcaggcat gttatcccct tgtgaaggga tagaggtgaa gcccgccaca
    15361 atcgcgtcgt ccggggtctg aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat
    15421 ccagattgtt gtgttgttgc catggtattc tccgtaccct tataaaaatg ttgcgcaatg
    15481 ttaactatag tcagcatgca acaaatcaca ttgcctgaat cggctcatct tttatgcagt
    15541 cctgcagaat gaagagtgat ttatgtgata tgaatcactt ttggtgggta aatttatgca
    15601 acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt
    15661 ctttttgctt cttctgacta aaccgattca cagaggagtt gtatatgtcc aagtctgatg
    15721 tttttcatct cggcctcact aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg
    15781 gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga taagccggtt aagctggcat
    15841 ctcaccgcga attcactacc tggcgtgcag agctggatgg taaacctgtt atcgtctgct
    15901 ctaccggtat cggcggcccg tctacctcta ttgctgttga agagctggca cagctgggca
    15961 ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca gccgcatatt aatgtgggtg
    16021 atgtcctggt taccacggcg tctgtccgtc tggatggcgc gagcctgcac ttcgcaccgc
    16081 tggaattccc ggctgtcgct gatttcgaat gtacgactgc gctggttgaa gctgcgaaat
    16141 ctattggcgc gacaactcac gttggcgtga cagtttcttc tgataccttc tacccaggtc
    16201 aggaacgtta cgatacttac tctggtcgcg tagttcgtca ctttaaaggt tctatggaag
    16261 agtggcaggc gatgggcgta atgaactatg aaatggaatc tgcaactctg ctgaccatgt
    16321 gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt tatcgttaac cgcacccagc
    16381 aagagatccc gaatgctgag acgatgaaac aaaccgaaag ccatgcggtg aaaatcgtgg
    16441 tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt ctgaaggccg acgcgttcgg
    16501 ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt tccaactgga cgtttgtaca
    16561 gcacaattct attttgtgcg ggtaagttgt tgcgtcagga ggcgttgtgg atttctcaat
    16621 catggtttac gcagttattg cgttggtggg tgtggcaatt ggctggctgt ttgccagtta
    16681 tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt
    16741 aagcgcggca aaacaacaaa ttacccaaag cgagcactgg cgtgcagagt gtgaattgct
    16801 caataacgaa gtgcgcagcc tgcaaagtat taacacctct ctggaggccg atctgcgtga
    16861 agtaaccacg cggatggaag ccgcacagca acatgctgac gataaaattc gccagatgat
    16921 taacagcgag cagcgcctca gtgagcagtt tgaaaacctc gccaaccgta tttttgagca
    16981 cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct
    17041 acgtgaacaa ctggacggtt tccgccgtca ggttcaggac agcttcggta aagaagcaca
    17101 agaacgccat accctgaccc acgaaattcg caatctccag caactcaacg cgcaaatggc
    17161 ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac aataaaaccc agggcaactg
    17221 gggcgaggta gtattgacgc gggtgctgga ggcttccggt ctgcgtgaag ggtatgaata
    17281 tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt
    17341 gcggctgccg cagggaaaag atgtggtgat cgacgccaaa atgacgctgg tcgcctatga
    17401 acgctatttt aacgccgaag acgactacac ccgcgaaagc gcgctacagg aacatatcgc
    17461 gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat tatcaacagc tgccggggct
    17521 gcgaactctg gattacgtgc tgatgtttat tcccgttgaa cccgcttttt tactggcgct
    17581 tgaccgccag ccggagctga tcaccgaagc gttgaaaaac aatatcatgc tggttagccc
    17641 gactacgctg ctggtggcgt tgcgtactat tgccaacctg tggcgttatg agcatcaaag
    17701 ccgtaacgcc cagcaaatcg ccgatcgtgc cagcaagctg tacgacaaga tgcgtttgtt
    17761 catcgatgac atgtccgcga ttggtcaaag tctcgacaaa gcgcaggata actaccgcca
    17821 ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg gcgcaggcag aagcgtttcg
    17881 cggtttagga gtagaaatta aacgcgagat taatccggat ttggctgaac aggcggtgag
    17941 ccaggatgaa gagtatcgac ttcggtcggt tccggagcag ccgaatgatg aagcttatca
    18001 acgcgatgat gaatataatc agcagtcgcg ctagcccatt aggggtagtt aagccgggta
    18061 gaaatctagg gcatcgacgc ccaatctgtt acacttctgg aacaattttt tgatgagcag
    18121 gcattgagat ggtggataag tcacaagaaa cgacgcactt tggttttcag accgtcgcga
    18181 aggaacaaaa agcggatatg gtcgcccacg ttttccattc cgtggcatca aaatacgatg
    18241 tcatgaatga tttgatgtca tttggtattc atcgtttgtg gaagcgattc acgattgatt
    18301 gcagcggcgt acgccgtggg cagaccgtgc tggatctggc tggtggcacc ggcgacctga
    18361 cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg
    18421 aatccatgct caaaatggga cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg
    18481 agtatgttca ggcgaacgct gaggcgctgc cgttcccgga taacaccttt gattgcatca
    18541 ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa agcactgcgt tcaatgtatc
    18601 gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc
    18661 cgctgagcaa agcctatgat gcatactcct tccatgtgct gccgcgtatt ggctcactgg
    18721 tcgcgaacga cgccgacagc taccgttatc tggcagaatc catccgtatg catcccgatc
    18781 aggataccct gaaagccatg atgcaggatg ccggattcga aagtgtcgac tactacaatc
    18841 tgacggcagg ggttgtggcg ctgcatcgtg gttataagtt ctgacaggag accggaaatg
    18901 ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc
    18961 tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta aagtattgcg cgtggaggta
    19021 aaaggctttt cgacgtcatt gattctggtg ttcagcgaac gccaggttga tgtactgggc
    19081 gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg ccagtgtgtt gccgaaactt
    19141 cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg agctggaagt gcagggcgat
    19201 attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg cagagttcga ccctgcggaa
    19261 ctgctggccc cttataccgg tgatatcgcc gctgaaggaa tcagcaaagc catgcgcgga
    19321 ggcgcaaagt tcctgcatca cggcattaag cgccagcaac gttatgtggc ggaagccatt
    19381 actgaagagt ggcgtatggc acccggtccg cttgaagtgg cctggtttgc ggaagagacg
    19441 gctgccgtcg agcgtgctgt tgatgccctg accaaacggc tggaaaaact ggaggctaaa
    19501 tgacgccagg tgaagtacgg cgcctatatt tcatcattcg cactttttta agctacggac
    19561 ttgatgaact gatccccaaa atgcgtatca ccctgccgct acggctatgg cgatactcat
    19621 tattctggat gccaaatcgg cataaagaca aacttttagg tgagcgacta cgactggccc
    19681 tgcaagaact ggggccggtt tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc
    19741 tttttccacc gcatattgcc gatcagctgg cgttattgca ggacaaagtt gctccgtttg
    19801 atggcaagct ggcgaagcag cagattgaag ctgcaatggg cggcttgccg gtagaagcgt
    19861 ggtttgacga ttttgaaatc aagccgctgg cttctgcttc tatcgcccag gttcataccg
    19921 cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt catccgcccg gatattttgc
    19981 cggtcattaa agcggatctg aaacttatct accgtctggc tcgctgggtg ccgcgtttgc
    20041 tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg cgagtacgaa aagaccttga
    20101 ttgatgaact gaatttgctg cgggaatctg ccaacgccat tcagcttcgg cgcaattttg
    20161 aagacagccc gatgctctac atcccggaag tttaccctga ctattgtagt gaagggatga
    20221 tggtgatgga gcgcatttac ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg
    20281 gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact caggtctttc
    20341 gcgacagctt tttccatgcc gatatgcacc ctggcaacat cttcgtaagc tatgaacacc
    20401 cggaaaaccc gaaatatatc ggcattgatt gcgggattgt tggctcgcta aacaaagaag
    20461 ataaacgcta tctggcagaa aactttatcg ccttctttaa tcgcgactat cgcaaagtgg
    20521 cagagctaca cgtcgattct ggctgggtgc caccagatac caacgttgaa gagttcgaat
    20581 ttgccattcg tacggtctgt gaacctatct ttgagaaacc gctggccgaa atttcgtttg
    20641 gacatgtact gttaaatctg tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc
    20701 aactggtgtt actccagaaa accctgctct atgtcgaagg ggtaggacgc cagctttatc
    20761 cgcagctcga tttatggaaa acagcgaagc ctttcctgga gtcgtggatt aaagatcagg
    20821 tcggtattcc tgccctggtg agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa
    20881 tgccagaact gcctgaactg gtttacgaca gtttgcgcca gggcaagtat ttacagcaca
    20941 gtgttgataa gattgcccgc gagcttcagt caaatcatgt acgtcaggga caatcgcgtt
    21001 attttctcgg aattggcgct acgttagtat taagtggcac attcttgttg gtcagccgac
    21061 ctgagtgggg gctgatgccc ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg
    21121 gttggcgcaa aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc
    21181 ggctttgttt aatcatcatc taccacagag gaacatgtat gggtggtatc agtatttggc
    21241 agttattgat tattgccgtc atcgttgtac tgctttttgg caccaaaaag ctcggctcca
    21301 tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac
    21361 caaagcagga taaaaccagt caggatgctg attttactgc gaaaactatc gccgataagc
    21421 aggcggatac gaatcaggaa caggctaaaa cagaagacgc gaagcgccac gataaagagc
    21481 aggtgtaatc cgtgtttgat atcggtttta gcgaactgct attggtgttc atcatcggcc
    21541 tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa aacggtagcg ggctggattc
    21601 gcgcgttgcg ttcactggcg acaacggtgc agaacgaact gacccaggag ttaaaactcc
    21661 aggagtttca ggacagtctg aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg
    21721 aactgaaagc gtcgatggat gaactacgcc aggccgcgga gtcgatgaag cgttcctacg
    21781 ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac catccataac ccggtggtga
    21841 aagataatga agctgcgcat gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt
    21901 cgccggaaca gaagccagaa accacgccag agccggtggt aaaacctgct gcggacgctg
    21961 aaccgaaaac cgctgcacct tccccttcgt cgagtgataa accgtaaaca tgtctgtaga
    22021 agatactcaa ccgcttatca cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat
    22081 tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc gccaatgaca tctatcactt
    22141 ggtatccgcg ccattgatca agcagttgcc gcaaggttca acgatgatcg ccaccgacgt
    22201 ggcctcgccg ttctttacgc cgatcaagct gacctttatg gtgtcgctga ttctgtcagc
    22261 gccggtgatt ctctatcagg tgtgggcatt tatcgcccca gcgctgtata agcatgaacg
    22321 tcgcctggtg gtgccgctgc tggtttccag ctctctgctg ttttatatcg gcatggcatt
    22381 cgcctacttt gtggtctttc cgctggcatt tggcttcctt gccaataccg cgccggaagg
    22441 ggtgcaggta tccaccgaca tcgccagcta tttaagcttc gttatggcgc tgtttatggc
    22501 gtttggtgtc tcctttgaag tgccggtggc aattgtgctg ctgtgctgga tggggattac
    22561 ctcgtcagaa gacttacgca aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg
    22621 gatgttgctg acgccgccgg atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg
    22681 tctgtttgaa atcggtgtct tcttctcacg cttttacgtt ggtaaagggc gaaatcggga
    22741 agaggaaaac gacgctgaag cagaaagcga aaaaactgaa gaataaattc aaccgcccgt
    22801 cagggcggtt gtcatatgga gtacaggatg tttgatatcg gcgttaattt gaccagttcg
    22861 caatttgcga aagaccgtga tgatgttgta gcgcgcgctt ttgacgcggg agttaatggg
    22921 ctactcatca ccggcactaa cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag
    22981 tattcgtcct gttggtcaac ggcgggcgta catcctcacg acagcagcca gtggcaagct
    23041 gcgactgaag aagcgattat tgagctggcc gcgcagccag aagtggtggc gattggtgaa
    23101 tgtggtctcg actttaaccg taacttttcg acgccggaag agcaggaacg cgcttttgtt
    23161 gctcagctac gcattgccgc agaattaaac atgccggtat ttatgcactg tcgcgatgcc
    23221 cacgagcggt ttatgacatt gctggagccg tggctggata aactgcctgg tgcggttctt
    23281 cattgcttta ccggcacacg cgaagagatg caggcgtgcg tggcgcgtgg aatttatatc
    23341 ggcattaccg gttgggtttg cgatgaacga cgcgggctgg agctgcggga attgttgccg
    23401 ttgattccgg cggagaaatt gctgatcgaa acagatgcgc cgtatctgct cccgcgcgat
    23461 ctcacgccaa agccatcatc ccggcgcaac gagccagccc atctgcccca tattttgcaa
    23521 cgtattgcgc actggcgtgg agaagatgcc gcatggctgg ctgccaccac ggatgccaat
    23581 gtcaaaacac tgtttgggat tgcgttttag agtttgcgga actcggtgtt cttcacactg
    23641 tgcttaatct ctttattaat aagattaagc aatagcatgg agcgagcctc accatcgggt
    23701 tcggtgaaaa tggcctgaaa gccttcgaac gcgccttcgg taataatcac cttatcaccc
    23761 ggataagggg gggccggatc gacaatgtct ttcggtttat ataccgatag ctgatgaatt
    23821 accgccgatg ggactatcgc tggcgacgcg ccaaagcgca caaagtggct gacgccacgc
    23881 gtagcgctga tggtcgtggt atgaatcact tctggatcaa attccacaaa caggtagtta
    23941 gggaataacg gttcactgac tgcggtacgt ttaccccgta cgattttttc cagggtgatc
    24001 atcggagcga ggcaattaac cgcctgtctc tcaaggtgtt cctgggcacg ttgaagttgc
    24061 ccgcgcttgc agtacagtaa ataccaggat tgcataatga ctcttatccg tttaatcggg
    24121 gcgcaaggat agcaaaagct ttacgctaag ttaattatat tccccggttt gcgttatacc
    24181 gtcagagttc acgctaattt aacaaattta cagcattgca aagatgaacg ccgtataatg
    24241 ggcgcagatt aagaggctac aatggacgcc atgaaatata acgatttacg cgacttcctg
    24301 acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg
    24361 gaaatcactg aaattgctga ccgcactctg cgtgctggtg ggcctgcgct gttgttcgaa
    24421 aaccctaaag gctactcaat gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg
    24481 gcgatgggca tggggcagga agatgtttcg gcgctgcgtg aagttggtaa attattggcg
    24541 tttctgaaag agccggagcc gccaaaaggt ttccgcgacc tgtttgataa actgccgcag
    24601 tttaagcagg tgttaaacat gccgacaaag cgactgcgtg gtgcgccctg ccaacaaaaa
    24661 atcgtctctg gcgatgacgt cgatctcaat cgcattccca ttatgacctg ctggccggaa
    24721 gatgccgcgc cgctgattac ctgggggctg accgtaacgc gcggcccgca taaagagcgg
    24781 cagaatctgg gcatttatcg ccagcagctg attggtaaaa acaaactgat tatgcgctgg
    24841 ctgtcgcatc gcggcggcgc gctggattat caggagtggt gtgcggcgca tccgggcgaa
    24901 cgtttcccgg tttctgtggc gctgggtgcc gatcctgcca cgattctcgg tgcagtcacc
    24961 cccgttccgg atacgctttc agagtatgcg tttgccggat tgctacgcgg caccaaaacc
    25021 gaagtagtaa agtgtatttc caatgacctc gaagtgccag ccagtgccga aatcgtcctg
    25081 gaagggtata tcgatcctgg tgagatggcg ccggaagggc cgtatggtga ccacacaggt
    25141 tactataatg aagtcgataa tttcccggtg tttaccgtga cgcatattac ccagcgtgaa
    25201 gatgcgattt accattccac ctataccggg cgtccgccag atgagcccgc ggtactgggc
    25261 gtggcgttga acgaagtgtt tgtaccgatt ctgcaaaaac agttcccgga aattgtcgat
    25321 ttttacctgc cgccggaagg ctgttcttat cgtctggcgg tagtgacgat caaaaaacag
    25381 tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg
    25441 tacactaaat ttgtgatcgt ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg
    25501 atttgggcga ttaccacccg tatggacccg gcgcgggata ctgttctggt agaaaatacg
    25561 cctattgatt atctggattt tgcctcgcct gtctctgggc tgggttcaaa aatggggctg
    25621 gatgccacga ataaatggcc gggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa
    25681 gatccagatg ttgtcgcgca tattgacgcc atctgggatg aactggctat ttttaacaac
    25741 ggtaaaagcg cctgatgcgc gtttgttttg ccctatttat cgatccgaca gagaaagcgc
    25801 atgacaacct taagctgtaa agtgacctcg gtagcagcta tcacggatac cgtatatcgt
    25861 gtccgcatcg tgccagacgc ggccttttct tttcgtgctg gtcagtattt gatggtagtg
    25921 atggatgagc gcgacaaacg tccgttctca atggcctcga cgccggatga aaaagggttt
    25981 atcgagctgc atattggcgc ttctgaaatc aacctttacg cgaaagcagt catggaccgc
    26041 atcctcaaag atcatcaaat cgtggtcgac cttccccacg gagaagcgtg gctgcgcgat
    26101 gatgaagagc gtccgatgat tttgattgcg ggcggcaccg ggttctctta tgcccgctcg
    26161 attttgctga cagcgttggc gcgtaaccca aaccgtgata tcaccattta ctggggcggg
    26221 cgtgaagagc agcatctgta tgatctctgc gagcttgagg cgctttcgtt aaagcacctt
    26281 ggtctgcaag tggtgccggt ggttgaacaa ccggaagcag gctggcgtgg gcgtactggt
    26341 accgtgttaa cggcggtatt gcaggatcat ggtacgctgg cagagcatga tatctatatt
    26401 gccggacgtt ttgagatggc gaaaattgcc cgtgacctgt tttgcagtga gcgtaatgcg
    26461 cgggaagatc gcctgtttgg cgatgcgttt gcatttatct gagatataaa aaaacccgcc
    26521 cctgacaggc gggaagaacg gcaactaaaa ctttattcag tggcatttag atctatgtcg
    26581 catccggcaa ttggtgcacg atgcctgatg cgacgctggc gcgtcttatc aggcctacat
    26641 tggtgccgga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
    26701 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
    26761 tgaacgtctt tgcgttccat cagattcagc agcgtggtgc tgatacgcgc accggaacaa
    26821 cccagcggat gacccagcgc gatcgcacca ccattgaggt tgatcttctc gtcaatctgc
    26881 tccattagcc cgagatcttt aatgcacggc aggatctgcg cggcaaaggc ttcgttcatc
    26941 tcaaacacgc cgatatcgct ggcagaaagc cccgcttttt tcagcgccag cttcgaggcc
    27001 ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat cgaacgcaca
    27061 cgagcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac cagcatggca
    27121 gctgcgccat cggaaagtgc agaagatgtg cccgccgtta ccgtaccgtt tactggatca
    27181 aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
    27241 taattaaact gcttcaggac gccgtcggca tcgtgaccac cagttgggat gatttcattt
    27301 ttaaatgcgg ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
    27361 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt taagcccatc
    27421 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac gccgtgactc
    27481 atcggcacat ggcctatatg ctccacgccg ccaaccagac atgcctgcgt atcgccagtc
    27541 atgatcattc gtgctgcgtc atgcagtgcc tgcatggatg aaccacacaa gcgattgacg
    27601 gtaaccgccg ggacagagtg cggcacttct gccagcagcg ccgcgttacg ggcgatgtta
    27661 aaaccctgct ccagcgtctg ttgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
    27721 tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttccgca
    27781 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
    27841 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
    27901 ggctcaaccg gaggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
    27961 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
    28021 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
    28081 accagcgcca tatccgcttc cgccggagtg gcgataatgc cctcttccag acagcgcacc
    28141 acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct gaaatcgcgc
    28201 ttcggttggc tcacttctgc cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc
    28261 ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
    28321 ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt tctgcatccg ctgcgggaag
    28381 cctgctgcca tgacagcctg agcgtgatgc gcggtatcaa tgcccacaac gtccagcaga
    28441 tatgccgggc ccatcggcca gccaaactgt ttttccatca ctttgtcgat cttgcggaaa
    28501 tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat acgggaacag cacgcggtta
    28561 acaaagaagc cggggcagtc gttaaccaca atcggcgtct tgcccatctt gctcgcccag
    28621 gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaataat ttctaccaac
    28681 ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga agttttccgg gcgttccagc
    28741 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtatcctgg
    28801 cgtacttttt gttcggtttc tgccagtacg gcttttttca ctttcgggtt ttcaacaacc
    28861 gcttctacca caatatccac gcggtcaaat ccggcgtagt cgagcgttgg gtggatcgtg
    28921 gagatcacgc cagccagttt cagaccatcg attttgccgc gctcaagctg cttgttcagc
    28981 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
    29041 accggcacgc ctttccacgc ggactggtaa gcgataccgc cacccatgat gcctgcgccc
    29101 agcacagcag cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
    29161 acatattgat cgttaaggaa aatgccgacc agtgcgcgag cttcgttggt atgcgccagc
    29221 gggacaaaac ttttgttttc cagatttaag gcttcttcac gaccaaaacg ggccgcagct
    29281 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgccgt ttgcgcgacc
    29341 attcctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagcggttcc
    29401 tgcttcggct gacgtttcgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaatc
    29461 gccatcgccc cttcaatcag tttttctgct ttgactacgc catccaccag accgattttc
    29521 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagtgc actgtcagcg
    29581 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
    29641 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
    29701 tcgcagccac cacccagcgc atagccgttg acggcagcaa tggtcggcac cggcagatct
    29761 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
    29821 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
    29881 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
    29941 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
    30001 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
    30061 gtctcctgaa tccgcaaggt aatctggtcg taccagatgc atcggagtgt gtgttttgtg
    30121 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
    30181 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
    30241 aaaaaatgga atcactggcc tcgctctata aaaatcatat agctacctta caagaacgga
    30301 ctcgcgatgc gctggcgcgc ttcaagctgg atgcattact tattcactcc ggcgagctgt
    30361 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
    30421 gggtgccggt aactcaggtg ccaaactgct ggttgctggt ggatggcgtg aacaagccga
    30481 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctcct
    30541 tctggactga agatgtggaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
    30601 tgcctgctgc gcgcggcaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
    30661 tgggtattga ggccagcaac atcaacccga aaggggtgat cgactacctg cattattacc
    30721 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
    30781 atggtcatcg tgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaata
    30841 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
    30901 cgcttaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
    30961 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg gctgacctga
    31021 cccgtacctg gtcggcaaaa agcgacaacg actacgcaca gctggtgaaa gacgtaaatg
    31081 atgaacaact ggcgctgatc gccaccatga aagcaggcgt cagctatgtg gattaccaca
    31141 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
    31201 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtattggcc
    31261 atccgctggg cctgcaggtg catgacgtcg ccggttttat gcaggatgat agcggtacac
    31321 acctcgcggc accggcaaaa tatccgtacc tgcgctacac ccgtattctc cagccgggca
    31381 tggtgttaac catcgaaccg ggtatctact tcattgaatc gcttctggca ccgtggcgtg
    31441 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
    31501 gcattcgtat cgaagacaac gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg
    31561 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
    31621 agagatcaaa aagagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
    31681 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
    31741 gtgggtggcg ggtgcgccgg atgattctca acagctgggt ttctctgacg acggggagcc
    31801 ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat
    31861 taccgctgtg gtagtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
    31921 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
    31981 attaaccgaa tatactttgc aatgtgaata tagtcagtta accggcattg aagcgttgct
    32041 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
    32101 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
    32161 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
    32221 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
    32281 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
    32341 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg gtggccgaac
    32401 cgcaaagaga agggcgaact gaaatcccgt gaggggtttc tgatagtggt gctgttttgg
    32461 accgtgctgg gtagtgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
    32521 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
    32581 gtggggctgg attcgctccc tcatgccatc ctcttttatc gccagatgct gcaatggttt
    32641 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
    32701 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
    32761 attgcggaaa cggcgaaaac tctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
    32821 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
    32881 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga cagtccgact
    32941 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
    33001 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
    33061 atcggcgtac agtttacgct ggtggttatt tgtacactcg tactgtggtt tcataatgtc
    33121 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtatc gatggcgaca
    33181 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
    33241 ttatgttcag cttttatcgg cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc
    33301 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
    33361 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
    33421 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
    33481 acgggcgtgg atgatttttc tgcctttgcc tcagttgttg cgacattgaa taacctgggg
    33541 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
    33601 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattactggt gctctttacc
    33661 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
    33721 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
    33781 tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aactatgacc
    33841 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
    33901 tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
    33961 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
    34021 tgaactcgca atggcgtccc gatcgctgcg cggtcattgc cggggcgctg cgttacccac
    34081 gttatcgctg gtacgaccgt tttatgctca agctgattat gaagatgtca ggcggtgaaa
    34141 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
    34201 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc
    34261 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
    34321 accactgaca cggaacaacg gcaaacacgc agccgggtca gcggggttct cctgagaatt
    34381 ccgacagaga aagcaaaaat aaatgcttga ctttgtagcg ggaaagcgta ttatgaatac
    34441 cccgcgc
//
LOCUS       DAJTRD010000037        34141 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTRD010000037 DAJTRD010000000
VERSION     DAJTRD010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.037"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..513)
                     /gene="istA"
                     /locus_tag="OO315_001946"
     CDS             complement(<1..513)
                     /gene="istA"
                     /locus_tag="OO315_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCR5890871.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVPGYSRMLYIEFTDNMR
                     YDTLETCHRNA"
     gene            1129..1266
                     /locus_tag="OO315_001947"
     CDS             1129..1266
                     /locus_tag="OO315_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890872.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1866..2645
                     /gene="ipaJ"
                     /locus_tag="OO315_001948"
     CDS             1866..2645
                     /gene="ipaJ"
                     /locus_tag="OO315_001948"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5890873.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3589..4518)
                     /gene="virB"
                     /locus_tag="OO315_001949"
     CDS             complement(3589..4518)
                     /gene="virB"
                     /locus_tag="OO315_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5890874.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4735..4971)
                     /locus_tag="OO315_001950"
     CDS             complement(4735..4971)
                     /locus_tag="OO315_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5890875.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4975..6876)
                     /gene="ipaA"
                     /locus_tag="OO315_001951"
     CDS             complement(4975..6876)
                     /gene="ipaA"
                     /locus_tag="OO315_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5890876.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6885..7883)
                     /gene="ipaD"
                     /locus_tag="OO315_001952"
     CDS             complement(6885..7883)
                     /gene="ipaD"
                     /locus_tag="OO315_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5890877.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7934..9025)
                     /gene="ipaC"
                     /locus_tag="OO315_001953"
     CDS             complement(7934..9025)
                     /gene="ipaC"
                     /locus_tag="OO315_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5890878.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(9045..10787)
                     /gene="ipaB"
                     /locus_tag="OO315_001954"
     CDS             complement(9045..10787)
                     /gene="ipaB"
                     /locus_tag="OO315_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5890879.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10793..11260)
                     /gene="ipgC"
                     /locus_tag="OO315_001955"
     CDS             complement(10793..11260)
                     /gene="ipgC"
                     /locus_tag="OO315_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5890880.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(11317..11943)
                     /gene="ipgB1"
                     /locus_tag="OO315_001956"
     CDS             complement(11317..11943)
                     /gene="ipgB1"
                     /locus_tag="OO315_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5890881.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11959..12348)
                     /gene="ipgA"
                     /locus_tag="OO315_001957"
     CDS             complement(11959..12348)
                     /gene="ipgA"
                     /locus_tag="OO315_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5890882.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(12361..13845)
                     /gene="icsB"
                     /locus_tag="OO315_001958"
     CDS             complement(12361..13845)
                     /gene="icsB"
                     /locus_tag="OO315_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5890883.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            14159..15775
                     /locus_tag="OO315_001959"
     CDS             14159..15775
                     /locus_tag="OO315_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5890884.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAGVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15785..16147
                     /gene="ipgE"
                     /locus_tag="OO315_001960"
     CDS             15785..16147
                     /gene="ipgE"
                     /locus_tag="OO315_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5890885.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            16147..16605
                     /gene="ipgF"
                     /locus_tag="OO315_001961"
     CDS             16147..16605
                     /gene="ipgF"
                     /locus_tag="OO315_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5890886.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16614..17729
                     /gene="mxiG"
                     /locus_tag="OO315_001962"
     CDS             16614..17729
                     /gene="mxiG"
                     /locus_tag="OO315_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5890887.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17737..17988
                     /gene="mxiH"
                     /locus_tag="OO315_001963"
     CDS             17737..17988
                     /gene="mxiH"
                     /locus_tag="OO315_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5890888.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            18001..18294
                     /gene="mxiI"
                     /locus_tag="OO315_001964"
     CDS             18001..18294
                     /gene="mxiI"
                     /locus_tag="OO315_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5890889.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            18300..19025
                     /gene="mxiJ"
                     /locus_tag="OO315_001965"
     CDS             18300..19025
                     /gene="mxiJ"
                     /locus_tag="OO315_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5890890.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            19022..19549
                     /locus_tag="OO315_001966"
     CDS             19022..19549
                     /locus_tag="OO315_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5890891.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19491..20186
                     /gene="mxiN"
                     /locus_tag="OO315_001967"
     CDS             19491..20186
                     /gene="mxiN"
                     /locus_tag="OO315_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5890892.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            20179..20586
                     /gene="mxiM"
                     /locus_tag="OO315_001968"
     CDS             20179..20586
                     /gene="mxiM"
                     /locus_tag="OO315_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5890893.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20570..20998
                     /gene="mxiM"
                     /locus_tag="OO315_001969"
     CDS             20570..20998
                     /gene="mxiM"
                     /locus_tag="OO315_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5890894.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            21128..21760
                     /gene="mxiE"
                     /locus_tag="OO315_001970"
     CDS             21128..21760
                     /gene="mxiE"
                     /locus_tag="OO315_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5890895.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21747..23447
                     /gene="mxiD"
                     /locus_tag="OO315_001971"
     CDS             21747..23447
                     /gene="mxiD"
                     /locus_tag="OO315_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5890896.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23466..24533
                     /gene="mxiC"
                     /locus_tag="OO315_001972"
     CDS             23466..24533
                     /gene="mxiC"
                     /locus_tag="OO315_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5890897.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24546..26606
                     /gene="mxiA"
                     /locus_tag="OO315_001973"
                     /gene_synonym="virH"
     CDS             24546..26606
                     /gene="mxiA"
                     /locus_tag="OO315_001973"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5890898.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26619..27020
                     /gene="spa15"
                     /locus_tag="OO315_001974"
                     /gene_synonym="spaK"
     CDS             26619..27020
                     /gene="spa15"
                     /locus_tag="OO315_001974"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5890899.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            27024..28316
                     /gene="spaL"
                     /locus_tag="OO315_001975"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             27024..28316
                     /gene="spaL"
                     /locus_tag="OO315_001975"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5890900.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            28409..28747
                     /gene="spa13"
                     /locus_tag="OO315_001976"
                     /gene_synonym="spaM"
     CDS             28409..28747
                     /gene="spa13"
                     /locus_tag="OO315_001976"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5890901.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28734..29612
                     /gene="spa32"
                     /locus_tag="OO315_001977"
     CDS             28734..29612
                     /gene="spa32"
                     /locus_tag="OO315_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5890902.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29606..30487
                     /gene="spa33"
                     /locus_tag="OO315_001978"
                     /gene_synonym="spaO"
     CDS             29606..30487
                     /gene="spa33"
                     /locus_tag="OO315_001978"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5890903.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30492..31142
                     /gene="spa24"
                     /locus_tag="OO315_001979"
                     /gene_synonym="spaP"
     CDS             30492..31142
                     /gene="spa24"
                     /locus_tag="OO315_001979"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5890904.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            31157..31417
                     /gene="spaQ"
                     /locus_tag="OO315_001980"
     CDS             31157..31417
                     /gene="spaQ"
                     /locus_tag="OO315_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5890905.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            31417..32187
                     /gene="spa29"
                     /locus_tag="OO315_001981"
                     /gene_synonym="spaR"
     CDS             31417..32187
                     /gene="spa29"
                     /locus_tag="OO315_001981"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5890906.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            32194..33222
                     /gene="spa40"
                     /locus_tag="OO315_001982"
                     /gene_synonym="spaS"
     CDS             32194..33222
                     /gene="spa40"
                     /locus_tag="OO315_001982"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5890907.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            33235..33477
                     /locus_tag="OO315_001983"
     CDS             33235..33477
                     /locus_tag="OO315_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890908.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10608 a         5025 c         6872 g        11636 t
ORIGIN      
        1 cgcattacga tggcaggtct ccagcgtgtc ataacgcata ttgtcagtga attcgatgta
       61 caacattcgg ctgtatccgg gaacagcaac gaacacgtga agcggtgagc gaccattacg
      121 catagtgccc cagtcaacct gcatctgtcg tccgggttca gtttcgaacc gaacggcagg
      181 ctcctgctcc tgaggaaccg agagagaacg aatgaatgcc ctgagaatgg tcattccgcc
      241 acgatatccc tggtctctga tctcgcgagc gattaccgtt gccgggattt tgtaaggatg
      301 agcatcggcg atgcgttgac gaatataatc ccggtattca tccaggagtg aagcaacagc
      361 aggtcgcggc gtatattttg gcggctcaga ttttgcctgc aaataacgtt taacggtatt
      421 gcgggagagc cccagttctc tggcaatcgt ccggctactc attccctgct tgtgcaggat
      481 tttaatttcc ataactgtct caaaagtgac cataagctct cctgaatcag gagagcagat
      541 taccccctgg atctgatttc aggcgttggg tgtggatcac tattgcaccg ttcgtgacac
      601 ttgttgacta cctgtgcggc atctggtatc aaggtggcgt gaagccggtt caggttctca
      661 tgcggaacgt tggaacctat atccccccga taccaaaggc gaaatgtaag tgacaaaatg
      721 caatgaacac gttctccgtt aactgaggag catggtgata aataaaggta aatgcagaag
      781 gtgtagagtc tcgcagattt ctgacatcat agagcctaac attagatgca taacgctaca
      841 gattgtagaa tctctacagg tgaaatatac actggttggc gtttatgtcg caaaacatgt
      901 cattcaggtt catctcacca ataaggatat gagtgaagtg gaggataagt gagtctgctg
      961 tcagagtttt ctggtgtatg tcagtaactg cgaaccatgt ctgcttgata tgggagctta
     1021 tggaggctct ctgtacaagg ttagggagct aacaaagcag ggataaaaag tccgcctgat
     1081 gcaggcacgg ttcgttaaat cttttgccat tggtaacaaa atggtgcgat gaatgttcag
     1141 gctatcttta tcttgatggt ggttcagctg tttggtaaag aaatcgctgt aacaatagaa
     1201 gaactgcagg cagtactggt cccacatcgt ttgctcagtc aactggtgaa gttccgtagc
     1261 gcgtaaatta atgctttgca tgaaacgtta ctggagtgga tgaaagaata cctgcatata
     1321 tcattactgc gttggattac cagtacaaca gattgaatga actggacatg ttgatagacg
     1381 gcattgaaaa gcagcttata agcatagtgc ggcagagtta attggagagc acttcgcctt
     1441 ctgagtatta agtaagcatg gtggtacctg cggatactat ctattcactg tgctcggtcg
     1501 gcagccttac tgaccaagga gttgggggag ttgataacaa agttgaaaaa acagctctca
     1561 accaaggtgg ttattgtaac aatagcaaat aagctggtgt gacctgtatc acaataaccg
     1621 attatggacg taaatacgac aaaaatatgt cagtatcagg cggtattaat tgctgatgct
     1681 gatggacatt aactcttaat aaaagggtgg atgctgaaag gttgttatgg tgacggacgt
     1741 gatgacgacg gaaaagagta tgaggttttg gatttaaagt tcgttcgggg aataaggacc
     1801 cggtcagcta attctcatag tagttcgcat tgctggatgc aatactgtgc tgcaattact
     1861 cgtcgatgtc ggaacaacgg aagccttgca aacggggatg tattcataca ggtgttatgt
     1921 tatatggggt tttattgcag ggggctatac caagagaata tatgatatct catcagactg
     1981 atgtgagggt aaatgagaat agagtaaacg aacaaggttg ttttttggcg aggaagcaga
     2041 tgtatgacaa ttcttgtgga gctgccagtt tactctgtgc tgcaaaggaa ttaggggttg
     2101 ataaaatacc acaatacaaa gggtctatgt ccgaaatgac aaggaagagt tctcttgatt
     2161 tggataatcg ttgtgagcgg gatttatact taataactag tggtaattat aatccgagaa
     2221 tacacaaaga taatatcgcg gatgccggtt attctatgcc tgataagata gtcatggcta
     2281 cgaggcttct tggattgaat gcttatgtcg ttgaagaaag taacattttt tcgcaagtaa
     2341 ttagttttat ttatcccgat gcaagggact tattaattgg catgggatgt aatatagtac
     2401 atcaacgaga tgtgttatct tctaaccaaa gagttttgga agctgttgct gtatccttta
     2461 ttggagttcc tgtcggcctc cactgggtgt tgtgtagacc tgatggaagc tatatggatc
     2521 cagctgtagg agaaaattat tcttgttttt caactatgga attaggtgca agaagaagta
     2581 attctaactt tataggatat actaaaatag gtatttccat agttataact aatgaggctt
     2641 tgtaaattgt cgttttttat ctgtgaagga gcttgaatat tttggggggg gcataacaaa
     2701 tgatattact gagtacaaat ttagctatat tgggcggatt agttgtgtgg gaagacgaaa
     2761 tgtttctctt gaaataagtg aactccctca aggaatgatt gtgtgcttaa atatttaagt
     2821 gctggtaagt ttgagaaggc tgacatattt tgcagagcaa acagtgattg acttggatga
     2881 tatttttatt ttagtcactc gttgtaattg gagtatgctt tattttattt gaaaatagat
     2941 gtattttata cccgcaattt aaactgtaag ccctatgagg ggtatggata ttataagtta
     3001 ctctttgacc aagcataact tttggggtta gaaaaataaa tctcttgtta ataattaata
     3061 tgattttaaa atcaaacttg tttataatta taaaataatg taagtcggtc attttatggg
     3121 aagattagga gaataaaaca gtaattatat ttgagaaatc tgctaatata ttcttaaagc
     3181 ttttttgata ctgtgaacga agtctgttaa tggtggattt tttgtttatc accatgtaaa
     3241 aaaaattgag tagtaaaagc gttgactgct aatcctcgct gtttttattg aaacgattaa
     3301 aaatacgctg aatattttcg gccagtcact catattcaca taaaattttg accatcatgc
     3361 cgcatccctt agttgcacaa ttcattttca atgatatgaa tattactaat ttcaccagat
     3421 aattttctcg tggtcagcag ggtatgagtt ttcaccgtaa ttttaaggaa acacagtgcg
     3481 tggggattga ataacagata gccgacggaa ttaccccacc ggctgaaagt caagttcaac
     3541 gaagattttt attatctgaa ttgggcagtt tacatcagtg ttcgatgttt atgaagacga
     3601 tagatggcga gaaattatat cccgaatagc ttcatctatt tctctctgaa ctgttttgtt
     3661 tattttagat aatgtaaata ttagagttct gtttgtttta ttctcttttc tttttatgta
     3721 agtccgtttg tctttagaaa taaataacga gtcaactttt agcgaagggt tttgctttct
     3781 gtttttgctt ttctttatga tgtttaaaat ttctttttta tatatgtccg ggggcaaatt
     3841 tgtatcaagg tcttttattt cttcctttaa tattggtagt gtagaactaa gagattcatt
     3901 agccttttca agtcctttat aataattgaa taatatcttg tagtcattaa agttaagttc
     3961 tgacgcgatt ggaaatagag atattatttc ctgtggaacg cttgctgcct gaaaggcacg
     4021 agtgactttc gcgcgagaca gattctcttt tttggctatg tctttatagg acatccctga
     4081 tactttcaga aaattaagac caataccaag ttctcggatg ctatgctctt ttgctgtttg
     4141 tatatcgttt gctagttttc tggcatcaag agtagatata tattcttttg aatatagaac
     4201 ttcaagatct gctcctgcat atattgcaga tgctcttcta cgagtgccat ccagaatttc
     4261 aattctacca tcaatctccc ttcctattac agggaagaat tgttgtagct ttatagtttt
     4321 tatgatatcg gctaatgatt cttctgttag ggattcttga tcgcgtacat tcgttttttg
     4381 gttgacgaag gttaaatctt ggatcctttt atgaggaatc ttggctttga taaaggaaac
     4441 tttattacca gaatggagtg taaactcttt cttttggcct tcctttatac ttaacaagtc
     4501 gttgcacaaa tccaccatat cacaccctgt ttattcatat tgatttatat tgacaaaagt
     4561 taaatgcagt gtgagcaaga ttgtatgtaa ctgttgatga tagtcaacgg tagtcttaaa
     4621 aattactatt ttaatagctg gattgatgca atttatcttt attggataaa agaataatat
     4681 tcttgtggag ctctgatgtg agagctccat agatgaaaaa atgcttatca attactatat
     4741 tgattttgta ctatttttga ctacagaaat aagatctcca atagtaataa tgtgttctaa
     4801 tgtagattca tctatacgta gattgaactc aaaactaatc atatccagca tttcaatcag
     4861 agaaagagaa tcggcagaaa gatcttcaat aaatttagtt tcttcagata gtttgctgta
     4921 cgctatacca tagcaaaagg ccactattga taatattttt tcttttatca tattttaatc
     4981 cttattgata ttctttaata cttttgatag ggaagtggtt acatcttttg ctgctttata
     5041 tatattattg ttttcagctg taatatcatt gttatttttt aaatcagata tatcatctgt
     5101 aagtaattct gcagaggtat cgtcaatttt tgacaatacc ttgctgaggg cgctagatac
     5161 ttcttttgcc ttttcataga tcgcatgatt tttatcaatt gtatctgcgt tggtttctgt
     5221 tacgtagttt cttatatttg catctgtaac ttttaatttg ttgtttattc tatgttctgt
     5281 tgtggatttt gtaatgtcct ttgatttttc ctgtaactct cgttcttgtg ttcctgattt
     5341 agttccaatg agagatataa gattacttag ggagtttgtt actttttttg aagcttcaaa
     5401 tatcgtttcc cgtgttaaat atgtgtctcc atttcttgat aaaaaatcag tctgaagtac
     5461 ttttgaatca gtgtttgagt tgaatttatt gatttgcgaa gctactctat gagaaagaga
     5521 agcgctactt ttagttgtat ctgatgagtt atcattactg cttgtcactc cttccgggtg
     5581 gagagggggg gtggttgaag agttctgtat gttcagaggc ctgtgtcccc gagaaagaga
     5641 gttatttgca gatctttctt gactactcgt agttaacagg tctgaaccta ttccatccga
     5701 attatctgga gtatggttga aagccttttc taaagctgaa ttatttgcaa atgtctggac
     5761 agtatctgat atagagtgtg gtagaacgga agttactttt tgaactagtg ctctttgtgg
     5821 atttagtaat gagtttccgt tacgggataa tagatcgttt gtctgagaag taggagactg
     5881 tgcatcattt tctgggttct cgtgatagct attgtcatat actctgttgt caaaaactct
     5941 attatcgtaa gttctattat cattatttat atgatagtga attacaggtt gggatgtttt
     6001 ctcattggct ccgggggttg gtccgccatc tggcatatct tcgggaggtg tagggaggcc
     6061 atcttggata ggatgcgaat cttttatatc ttctaaaata tcattaagga cttttctcaa
     6121 cagctcagga aacacggcta ctgtatttaa gtctcttgac agtttaccta taccaaatcc
     6181 tttactatca atagtatttt tacgttgatt gcgcaaacta tcaaaggcaa gttttgcctg
     6241 tgcgtcaact aaatcatcta gtttgagaga tgaaacagtt tcattactta actttaattc
     6301 agcaagaata ttattcttga taaatgatga tacttcagta ttgaggagtt tttgtcccaa
     6361 tgataaagct tcaccagatg tgtgttgttg tgctaatagt gcaagtctgt gctcaatgtt
     6421 ttgtcgtaat tgatggagtt tcttattctt tgccgaccat ggtccaaagg cagaatcttg
     6481 ggataattta tcttttataa ttagttccga atactgtgcg acaatttttt gaagtccctt
     6541 ttcgttttca aaattaagac cattttgatg tactaaggag tacaaaacat ttgaaaattc
     6601 gagaatctcg cttttggcat acgcattcat tgagttggaa taaatgcttc ttaacacatc
     6661 ttctttccct gaggaagaaa ataaaaaatc aagacatttc tgattaaaag acgttgcaaa
     6721 gttttctttt tcagacactg atgctggtgt ttttagagaa gtttgcgaac tatggatatc
     6781 ggatattttg ctttttaact cgctgtattc tgttgatgat ggtgaagttg ccttatataa
     6841 gaatgttggc gcttgagtat tatttacatt atgcatggcg cacctcagaa atggagaaaa
     6901 agtttatctg tatctgtaca tgagcttatt gtactactca aaacctttac taaattatca
     6961 aaaatactat tggcattact gtatttttga actaaagttt gaagattatt tttcattgtt
     7021 tcatcttcgg cagagaatcc ggcattccat gcctgatatt ttgcattatc tagcacaacc
     7081 tcgccatttc cacctagatt atctaagctt tttaacatat tgtctattgg ggtcatgttt
     7141 atactgacaa catatccccc gtttttttga gataccttgc cgattgttcc acctaattct
     7201 gtaagccatt tatttgcttg ttcctgacta acagtattat ttgctggata tagcggttta
     7261 tctttatatt tttccttgag ttcttccaat gcctttttaa gcgagttgac ttgtaatttc
     7321 acggagtttc cgtcgttacc tccgggagag atccagccgg caagactgga aagaacagcg
     7381 ctaaaatctt gatacatttg agtatatgaa ctaacggcat gttcatatac tttcagatac
     7441 tgttcattaa tatcattgat ggagtttgca attttagccc acagttctct atgagatatc
     7501 atttgatctc catcaagctc ggcttctttc ggagctgaat gtaataattc tcttgcgtct
     7561 ttattaattg gatattcgtg cctggaaaga atatctaata gttgagcgga tttatttaca
     7621 tccatgctaa tctgagatga atgtaatgct atttcttcta gcgatgtttt agtcaacgtt
     7681 ttttgtgaaa gctctttctt taatgcctga tttgttgttc tgatattatg aagggtgtcg
     7741 ttgagcatag taagggaact tacaggatga gaactggtcg ttgtttttat atcagaatta
     7801 actgtttcgg ttgatgaacc gttggtattg tttggactga atgatgaggt ggaaatacta
     7861 ttagtcagag ttgttatatt catggttatt tccttacagg aagagccata tattattatg
     7921 gctcttggat atattaagct cgaatgttac cagcaatctg actggctgtc gaattctttg
     7981 attggttgat gctgtcaatt atattcaata atttttgtat taattgattt gtcgcttggg
     8041 atgcttcttt agatacttgg gatgcttcct ctgcttgttt actgctggcc tgactgatta
     8101 gttgttcttc ttcttcaaga gcagatgcat aacgccctcc tgatgtggat atattaccag
     8161 caacggctga tgtttccatt gttgcacggc caatgttttg tttttgctgc gcagaaaggg
     8221 tattgagctc gtaagttctt ctctgggtgt caattttatc ctgcaaagaa atatcgggag
     8281 aactaagaga agttttatgt tcagttgaca gggatatatt atctggcgcc agtttatttt
     8341 tacctaaaaa ttttgtgcta gagttagttt gtggtgaggt gatatttgta tctatttgtt
     8401 tatttaaccc taatttagaa cctgcaagct ctttttcaag agattgagca gtggcaaggt
     8461 tctttcttaa ggctcctttt tggtcgctaa tccctgaatg cgtttttttg gcaccgatac
     8521 ccgttatacc tacttgtgtg actgctccag taatgcttga tgatagggct gccaggcctt
     8581 gccgaacaat gttctctgca gctgattttg tagcatcgaa cgcaatcaat gacaattgag
     8641 agcccaattt agtttctgca gtgcggagag cagaaagtag aacggctaca ctaataatta
     8701 aagaaacagc attagaagaa agactggaaa tatccagagt gttctctgga gtaagggtgt
     8761 gactcatctc tgaaagatct ttcatctttt tgtcagtaag gcgagcaatg atttctgaat
     8821 catgctgaac ctgctctgat aactttaaaa gttgatcatc atttaatgtg gttgttaata
     8881 cgggattttt accgacatta agtggaatat gcgctgcaat ctgctgataa ttttgtgatt
     8941 tttgtgtttc ggaagaactt tgagtttgtt ttgtggatat atctgtatat aaaatctggg
     9001 ttggttttgt gttttgaatt tccataacat tctccttatt tgtatcaagc agtagtttgt
     9061 tgcaaaattg cttttgcaac atcagttcta ttagcctgag agttggacat tgaggctaat
     9121 agatctgcaa ttacttcctg caattggccg aatttttcta ttgcttcact gatatatttt
     9181 gacagttgtt caacttgata tttcgataat gtcaggtctg ctagatttgt cgacgcgctg
     9241 ttctggaaaa cagcagaagc gacacttcct cccgcttgtg tggcagagtt aacactttct
     9301 cctaataaaa ctgcttggtt taaatgggtg gaaataattt gtttggatat tacttcatca
     9361 cccgctgcac caagaaattt atttaatctg gcaacagcat tagtgattaa atcgtccagt
     9421 tgagaagaaa aattcttgag aaactttggt ataaggtctg tgagggtttt acctattatt
     9481 ttgccaatat tttctgcaag ttttgctgct gcctgtttac caacagtggc tacgagaacg
     9541 actgctgcaa ctaggacaag agcgcctgcg attgccccca gaatagagcc aatcattttg
     9601 gcttttttcg agtcgacgcc caagccttcg agcatttttg taaatgcatc tgaaaggagt
     9661 ttgattaagg gttcaatgac tgctttcatg atcggattca gggcttgttc catgaaggaa
     9721 ttgccggtcg ctgcttgtac tatagcatcc gtaaccataa gagctaaacc aacagctgcc
     9781 agtgctagag aggctcctcc agaaaaagct gctgcaacaa cactaacgat agttaaaagt
     9841 gccccaagta ttttcccaac acaacccatt actctgttga gttcttctgc tttacgtact
     9901 tcagcagcat actcatcaga ttttctctcc atttcagttt ttcttgattc ttggagagac
     9961 tggaatagag ccagatcatt ttttaaagat tcttcattat tttttccaac tagttgaata
    10021 aaggttgcca tcaattgagt aacactggca agtccggtta atgatttctg ctgggttgat
    10081 agctgttcag cagatgctgt gtttgaaaat gcagaaaaag agtctatttc tttttcgagt
    10141 tgcattgatt tatctgtaag tttgctatga attgacaggg ttttctgctc aatcaatgtc
    10201 ctgtctttaa ctgctgcgtc ttttttgata gtgagttgta tttcttcccg gcttaatttt
    10261 ttcttttctg gtgactctgg gtcgagttcg gataatcttg tttgaatttg gttaatttta
    10321 ttttctaggt cttttatttt agaatctgcg ttttttagtt tattaatttg tttttcatag
    10381 tctctggtta gtccttcagt ttcagataga agagtgttaa ttttatcgga gaattctagg
    10441 tttttttgct gtcttgcctg ttgctgggac ttccaagcag taattttatt tgttaatgca
    10501 gttaaagatt tttcaccgag tatttgaata aggtttccaa ttaaaagcgt tagttgggaa
    10561 cttgcattta atgactttgg tgctttaagt tcagggatta atatatttga agttgagtgt
    10621 gcattagttg tattaatatt ttggttagca gtaagatcag caattgtaag agaaaataat
    10681 ttgttagctg catcatttgc agcttggata gtattgtctc caagctcagt ggaagccaat
    10741 attttggcaa gaggaaaacc agtggttgtg gtgcttacat tatgcataat aattactcct
    10801 tgatatcctg aattgcgtcc aagtatgatt gtgcttttat ttttaatttt tcatcattgc
    10861 tgtgttgaat tacgagttcg aagcactctt tagcttttaa gggggctttc aaccgaagct
    10921 ggcattgtcc agtatggaat actggtgtat agtcattttt tcctaatgca aaagcgacag
    10981 cataaaggtc tgctgcttgt tggaactgtt cttttatctg ataaatagct gcgagtccca
    11041 taatgtagtc tacattgtaa aagtcgtata tacataaaaa cctgaagaaa acttcagctt
    11101 cctctattct tcctttgttg taaaagtcat aagcatatga ataaatgtca tccatcatat
    11161 catcaggaat tgcattaata tctttcagtg tagcgccaga gttaattgca tcaattactg
    11221 cagtagagat gctttcattt tcggtgatat ttaaagacat aaggtctcct ttttgtctgc
    11281 tttactttaa taagtataag atttaatata aaagatttaa tttgtattgc tttgacggta
    11341 tacagccttt agaacctctg cgcttatttc tatttgtaaa acttttgttg attcattaaa
    11401 tattttatcg tagacggaac tggatactag atttgtaaga tccttcattt gtgtgttaaa
    11461 caaatccatg tcactgctat agcgttttaa aatctttgct ctgaggccag atgaggcaat
    11521 ctcgtttagt gctgtatgac tgatagatgt ttgtgctgat ttttcatcaa gttttatatc
    11581 cgggaatttt ttttcaatag ctgcaaatat gttttttttg ttatctgagg tcaaaacaac
    11641 tccattgtgt atggccattt catctatact tctgtttatc attgaagaga cataagtggt
    11701 tcgctcttgg ctcatccaac aaaatagttg attccccgaa ttactatctt ttattttgtc
    11761 tatgacgctg tccctgattt tatttatatt caccgggcat ccaaaattct tctgaggaaa
    11821 gcgttgtttt aagttaaaaa cagataatat tttctttaca agcttattgc gacttaacga
    11881 atcaaatgat gggcgaggaa tcgcgaattc aacctgtgga agtattttgt ttagaatttg
    11941 catgataccc cctatatgtt agttcacttc tgaagtgatg tttgcaatgg ttagtttaat
    12001 acaaaataaa aattcataca aagattcaat gagttcatca ggtgaaagat attttttgta
    12061 gtgtttcttg gtcaaaacac attgatgagt atcagggaga agacagatag aagaaccata
    12121 atctctactc aaagagtagt taaggatgag tatgtgttct ggattaataa tattttctgt
    12181 aaaaagttca tgttcattaa gaagtccggt caaaaagatg tattcatcat tatctgttat
    12241 acaaacaggt atttgttctt cgagcaatat aaaggcttga tttttatcgt taaaatcaag
    12301 ctttgcacct gttatttcat aaagtttatc atatagtttg cgacacattt ctatggcctt
    12361 ctatatatta gaatgagagt tattcaataa aagcttattg atctgtaata agtcgtgaat
    12421 gtaaagtttg gttccaagat ctggcgattt aagagaattg taataatcaa taatttttat
    12481 tgatttttcg atgatttctt cttttttatc tgttgcttcg gtttttaatt cgtttaatac
    12541 acgagtcata ttgctgggtg ctgattttac taagtatgag tggcaagcgt tgatgaccgc
    12601 atcaaagtct tctgaagata cattttcagt aggatgtttg taaagtgaaa ttgataaagg
    12661 cttgaatgtt tttggtgtgt ggataaggtt aattttattg aaacttttaa gatagctgcg
    12721 tcgtaattga gtcaatctgg aaagatcaaa atcagattca atatacttca ttatttcgtt
    12781 ataagactga ttgagacttt caatgcgttg cctaacttta tttgcatacg cgtatacatc
    12841 gttaggtgta gcaaccaatt taacgtcagg gagtggaaag tatatttcag aatttcctgc
    12901 tttaagaaca ttaagtgcca taccagcaca gttttcagat ttactgatta atttataatt
    12961 tgccaattgt ctgaaagcat ctgctttctt tttgcatata aattttgaca tgtttgtttc
    13021 atcaaggcca aaaaggttat ggctgatttt accatccttt gttttattat cacctgataa
    13081 tggaatgtat actttatcag cactgacccc ccaataacca taggggtctt ttttttgatt
    13141 agctctggga aaaaatattt ttttgttgtt attttgatct tgttttgaat ttaataagtt
    13201 tctaacctct tggcctgcat caagtctttc ggctgtttgg tctgaaatca tatttagttt
    13261 gtcttgctta taggtatcga cactgtaacc tgagcgtttt tcaaaaaggt agtttatttc
    13321 tgtatctttt gtaacattcc cgccgggata ccagctgaga taggtatgag ttggttcctg
    13381 tagtaaattg ttttttatac tgatactagt atgccctaaa tgggttatgc ctctatttcc
    13441 ccagcaacga aaaactaaat gtctttcaag atttttggct gtaccgatgc catgaaaacg
    13501 ctctgcggta ttcaacatta atttgatgtc tctacctgtg aaagagtgtt gaattgagtt
    13561 tttagctgct tcataggcgg caaacgcact ttctgcagat ttttcgctag ctaatgtatt
    13621 gaggaaaagc aataaggatt tttgattctc tagtcgataa ttttttagtt gttcatttaa
    13681 tatctgtgaa tttatctttg cattaatagt gcttaatgtt ctgaaaaaga gatcctttag
    13741 gttgaatttc tgtgctatca atataggtcc atgctctgta tcttcaacgc gtataggccc
    13801 ttttgtattg cttgcgtcaa tgaaattgct aattttgagg atcatacttt attaactctc
    13861 cattacttgg tgattgtggg gatgtaaaat gtatttggta tataatatat tcaattaata
    13921 aatttagaaa cttgagcctg ttaacataat caaattttct tttgctgtac ataatatgta
    13981 cctcgtgagc atatgtagtg ctcgtttcat catgaaatcc cacaagataa agtgcctgat
    14041 gtatcaggct cggagtgtta tagaaaaaga gagaaccctg ttgaataagt atcttttttg
    14101 ttgtaaaacg aggtacaaaa ctgcaatcaa tgccccattt atgactgagg ataattaaat
    14161 gcacataact aatttgggat tgcatcaggt ttcatttcaa agcggagatt cctataaagg
    14221 cgcagaagaa accggaaagc acaaaggtgt aagcgtgatt tcatatcaaa gagttaaaaa
    14281 tggagaaaga aataagggga tcgaagcgct taatcgatta tatttacaga accaaacaag
    14341 cttaacagga aaaagccttt tgtttgcgcg tgacagagct ggagtttttt gcgaggcaat
    14401 aaagttggca ggtggtgata cgtcaaaaat taaagccatg atggaacgat tagataccta
    14461 taaacttggt gaagttaata aaagacatat taatgagctt aataaagtaa taagtgaaga
    14521 gataagagca cagctaggca ttaaaaataa aaaggaattg caaacgaaaa taaaacaaat
    14581 atttactgat tatttaaata ataagaattg gggaccggtc gacaagaata ttagtcatca
    14641 tgggaaaaat tatggttttc aattaactcc tgcctctcat atgaagatag ggaataaaaa
    14701 tatatttgtc aaagagtata atggaaaagg aatttgttgt gcttctacga gagagagtga
    14761 tcatatcgcg aatatgtggc tttcaaaagt ggtggatgat gaggggaagg aaattttttc
    14821 gggtatcaga catggagtta tatcagccta tggattgaaa aaaaactcat ctgaaagagc
    14881 tgttgctgct cgtaataaag ctgaggagtt agtaagcgca gcattatata gcagaccaga
    14941 gttattatca caggctttgt ctggtaaaac agtagattta aagattgttt cgacttctct
    15001 tctgacgccg accagtttaa ccgggggaga ggagagtatg ttaaaagatc aggtgaacgc
    15061 attaaaaggg ttaaactcta aaagggggaa gccaacgaaa ttattaattc ggaatagtga
    15121 tggtcttctg aaagaagtaa gcgttaatct aaaagtggtg acatttaact ttggtgtaaa
    15181 tgaattagcg ctaaaaatgg gcttaggctg gaggaatgtt gacaagctta atgatgaatc
    15241 aatatgttct ttgttggggg ataatttcct caaaaatggt gtgattggag gttgggctgc
    15301 agaagcaata gaaaaaaatc caccatgtaa gaatgatgtg atatatctgg ctaaccagat
    15361 aaaagagatt gtaaccaaaa agttacaaaa aaatgataat ggagagccgt ataaattgtc
    15421 acaaagaatg actctattgg cttatactat aggtgctgta ccttgctgga attgtaagag
    15481 tgggaaggac agaacaggta tgcaggatgc tgaaattaaa agagaaataa taagaaaaca
    15541 tgaaacaggt cagttttctc aattaaatag taaattatcc tcagaagaga aaagattatt
    15601 ttctactatt ctaatgaata gtggtaatat ggaaatccaa gagatgaata ctggtgtgcc
    15661 cggaaataaa gtcatgaaaa aattgccgct atcttcgtta gagctatctt attctgaaag
    15721 aataggggac ccaaaaatat ggaatatggt gaaagggtat tcgtcatttg tataagagga
    15781 atatatggaa gatttagcag atgttatttg ccgagccttg ggtatccctt taattgatat
    15841 cgatgatcaa gcaatcatgc ttgatgatga tgtgctaata tatattgaaa aagaaggaga
    15901 ttcaataaat ttattatgcc ctttttgtgc attgcctgaa aatataaatg atcttatata
    15961 tgcgttgagc ctaaattact cagaaaagat atgcttagct actgatgatg aaggcggaaa
    16021 tctaattgcg cgattagatc ttaccgggat aaatgaattc gaggatgtat atgttaatac
    16081 agaatattat atttcgcgtg tgcgttggct gaaggatgaa tttgcgcgaa gaatgaaggg
    16141 gtattaatgt cccgtttcgt atttattttg ctgtgtttta tcccatattt agggagagct
    16201 gattgttggg ataaggctgg tgaaaggtac aatattccat caagtttgtt aaaagcgatt
    16261 gcggaaaaag aatccggatt taataaatct gcagtcaatg ttaataacaa tggaagtaaa
    16321 gattatggta taatgcaaat aaatgacttt cattctaaaa gacttagaga aatgggatat
    16381 tctgaggaaa tgctaattag tcatccatgt ctttctgtac attatgcagc aaagttatta
    16441 aatgagttta tgatgatgta tggaagaggg tgggaggctg ttggagccta taatgcagga
    16501 acgtctccga aaaagaaaaa agaaagatta aagtacgcag aggatatcta tagacgctat
    16561 ttgcggatag cagcagagag caagcagaat aatcgaagga tataagagga tttatgtctg
    16621 aggcaaagaa ctcaaatctt gcaccattca ggttattagt gaaactcaca aacggtgtcg
    16681 gggatgagtt tccgttatat tatgggaata acttaattgt tctcggacgg acgatagaaa
    16741 cacttgagtt tggaaacgac aatttcccag aaaatattat tcctgtaaca gatagtaaat
    16801 cggatggtat aatttatttg accataagca aagataatat ctgtcaattt agtgatgaaa
    16861 aaggtgagca aattgatatc aattcacaat ttaattcatt tgaatatgac gggatttctt
    16921 ttcaccttaa aaatatgagg gaggataaat cgagaggcca tatactaaac ggaatgtata
    16981 agaaccactc ggtgtttttt ttctttgctg ttattgttgt gttaattata attttttccc
    17041 tctctttaaa gaaagatgaa gtaaaagaaa ttgctgaaat tatagatgat aagagatatg
    17101 gaatagtcaa cactggacag tgcaattata ttcttgccga aacacaaaat gatgcggtat
    17161 gggcgtctgt agccttaaat aaaaccggat ttaccaaatg cagatatatt ctggtgtcaa
    17221 acaaagaaat aaatagaatt caacaatata tcaatcaacg attcccattt atcaatttgt
    17281 atgtgttgaa tttagtgagt gataaagcgg aattactggt gtttttaagt aaagaaagaa
    17341 atagttcaaa agataccgaa cttgataaat tgaaaaatgc tctgattgtt gaatttccgt
    17401 atatcaaaaa tattaaattc aactatctta gcgatcataa tgcaaggggg gatgctaaag
    17461 gtattttcac aaaggtaaat gtccagtata aagagatttg tgaaaataat aaagttacat
    17521 attctgtcag agaagaactg acagatgaaa agcttgagct cattaataga ctgatttctg
    17581 aacataaaaa tatatatgga gatcagtata ttgagttttc tgtacttttg atagatgatg
    17641 attttaaagg taaatcatat cttaacagca aagacagtta tgtaatgttg aatgataaac
    17701 actggttttt tttagataaa aataagtgag gataaaatga gtgttacagt accgaatgat
    17761 gattggacat tgagttcatt atctgaaact tttgatgatg gaactcaaac attacaaggt
    17821 gaactaacat tggcactaga taaattagct aaaaatcctt cgaatccaca gttgctggct
    17881 gaataccaaa gtaaattatc tgaatataca ttatatagga acgcgcaatc caatacagtg
    17941 aaagtgatta aggatgttga tgctgcaatt attcaaaact tcagataata gggagcattc
    18001 atgaattaca tttatccagt caatcaggtt gatattatca aagccagtga ttttcaatct
    18061 caagagatat caagtctgga agacgtcgtg tcggctaaat atagtgatat taagatggat
    18121 acagatattc aagtatcaca aataatggag atggtaagca atccagaatc attaaaccca
    18181 gaatctttgg ccaagttaca gacgacgctc tcaaattatt caataggagt atcattagct
    18241 ggcacgttag caagaaaaac agtttcggct gttgaaactt tattaaagtc ttaatttata
    18301 tgattaggta taaaggtttt attttattct tgttgctgat gttgattgga tgtgagcaac
    18361 gtgaagagtt aatttctaat ttatctcaaa gacaggcaaa tgaaataata tctgtgctag
    18421 aacgccataa tattactgct agaaaagttg atggaggtaa acaggggatc tcggtacaag
    18481 tcgaaaaggg gacatttgca tcggcagttg atttgatgcg catgtacgat ttgccaaatc
    18541 cggagagagt tgatatctca caaatgtttc ctacagattc attagtgtct tctccaagag
    18601 ctgaaaaggc ccgtttatat agtgctattg agcaacggct ggaacagtct ttagtttcta
    18661 ttggtggtgt tatttcggca aaaatacatg ttagctatga tcttgaagaa aaaaatatat
    18721 cttcaaaacc gatgcatata tcagtaatcg ctatatatga ctcaccgaaa gagtctgaac
    18781 tattagttag taatattaag cgatttttga aaaacacctt ttctgatgtt aagtatgaaa
    18841 atatatctgt catattaact ccgaaagaag aatatgttta tacaaatgta caacctgtta
    18901 aggaagttaa atcggaattt ttaacaaatg aagtaatata tttatttctc gggatggctg
    18961 tactagttgt cattcttttg gtatgggcat tcaaaacagg gtggttcaag agaaacaaaa
    19021 tatgataaga atggatggaa tttataaaaa atatctttca ataatttttg atccagcgtt
    19081 ctatataaat agaaatcggt tgaatttgcc ttctgaactg ttagaaaatg gcgtaatcag
    19141 aagtgagatt aataatctca taattaataa atatgatcta aattgcgata ttgaaccttt
    19201 aagcggggta accgctatgt ttgttgccaa ctggaattta cttccagctg ttgcttattt
    19261 tatcggtagt caagagtctc ggttaattaa tcatagtgag atggttatta gttattatgg
    19321 gggcaaaatc agtaagcaag gggaggccgc tattcgaagt ggtttttggc atcttattgc
    19381 gtggaaggaa aatatatctg ttggtatata tgaaagaata aacttattat ttaatccaat
    19441 tgctttggag ggaaattata cgcctgtaga aagaaatttg tcccgattaa atgaaggtat
    19501 gcaatatgca aaaaggcact ttaccggtat ccagacatca tgcctatgat ggtgttgtta
    19561 taaagagaat tgaaaaggaa ttatgtaaaa ccataaaaga tagagacact gagagtaaga
    19621 agaaagcaat ttgtgttata aaggaggcta cgaaaaaagc tgaatcactg cgtatagatg
    19681 ctgtatgtga tggttatcaa attggtatac agactgcttt tgagcatatt attgactata
    19741 tttgtgaatg gaagttaaag caaaatgaaa atagaagaaa tattgaggac tatataacta
    19801 gtttgctttc agaaaattta catgatgaga ggatcattag cactttattg gagcagtggc
    19861 ttagttcgtt gcgtaataca gtaacagaat taaaagtggt tttgcccaag tgtaatttag
    19921 cactgaggaa aaagttagaa cttgatctcc ataaatatcg ctcagatgtc aagattattt
    19981 taaaatattc tgaagggaat aattatatat tctgtagtgg taatcaagta gtggagttta
    20041 gtcctcagga tgtaatatca ggtgttaaaa tagaacttgc ggaaaaatta acaaaaaatg
    20101 ataaaaaata tttcaaagag ttagcacata agaaactgag acaaattgca gaagacttgt
    20161 taaaagagaa tcctgttaat gattaatcaa ataaatgcaa gcaatgctct gcagcaaaga
    20221 ttaaatagtg aagaagttgt taatttaaat gagagattat catcttcgca atcatttgat
    20281 gaggatataa tctatgaaat aatgcaatat ttctcacaga gtgaattaaa ttccatagat
    20341 aatgatgaac tgcacaataa gattgaacaa ttattcaatt cacgttttcc ttatcttaca
    20401 gctgcacaaa aaagttcact tcttaataaa ctcatagatg caaatcaata tgtcgatttg
    20461 catgagggat tttatgcatc attgtccata tataacaata ttgatttcta tattaagact
    20521 acaaccttcg attcattaat tagtgtcttt gaagcaggga gagaggcaga tgattcgaca
    20581 tggtagtaat aagttgaaaa tatttatttt aagtatattg ctattaacac tgagtgggtg
    20641 tgctttaaag tcatcatcta attctgaaaa agaatggcat attgttcctg taagtaagga
    20701 ttatttttct attccaaatg atttattatg gtcgtttaat acaaccaata aaagtataaa
    20761 tgtttactct aaatgtatta gtggtaaggc ggtttatagt tttaatgcag gtaaattcat
    20821 gggcaacttt aatgttaagg aagtagatgg gtgcttcatg gatgcacaaa agatagctat
    20881 agataaacta ttttctatgc tgaaagacgg ggttgtttta aaaggtaata agataaatga
    20941 taccatcctt atagagaagg atggggaagt taaattaaaa ttaattcgag ggatataatt
    21001 gtattgtgag taaatataaa ggtctaaata caagtaatat gttttacatt tactctagtg
    21061 gacatgaacc agttaacgtt gagcttgtaa aagataaaga acgtaacata attgagctgg
    21121 ctccagcatg gaagggtttt tttttgtccg aaatcaaaat ataaaattca gtgataatgt
    21181 gaactatcat tatcgcttta acattaatag ttgtgcaaaa tttcttgctt tttgggacta
    21241 tttcagtggt gctctagtag agcatagcca tgctgaaaaa tgtattcatt tctatcatga
    21301 gaatgatctt cgggatagtt gtaataccga atcgatgctt gataagttaa tgttaagatt
    21361 catcttttca tctgaccaga atgtaagtaa tgcactggct atgatacgaa tgactgagtc
    21421 atatcatttg gttctttatc ttctgagaac tattgagaaa gaaaaggagg taagaataaa
    21481 gagtctgaca gagcattatg gtgtgtcgga ggcttatttt agatctttat gtagaaaagc
    21541 tttaggggca aaagtcaaag aacaactgaa cacttggcgg ttagtaaatg gtttattaga
    21601 tgtatttttg cacaatcaaa caatcacttc agctgccatg aataatgggt atgcatcaac
    21661 atcccatttt tcaaatgaga taaaaacacg actaggcttt tctgcaagag aattatcgaa
    21721 tattactttt ttagtgaaaa aaataaatga aaaaatttaa tattaaatct ttgactctct
    21781 tgattgtatt gttaccattg atagttaatg ctaataatat tgactctcat ttattagaac
    21841 aaaatgacat agcgaaatat gttgctcaaa gtgatacggt aggatctttt tttgaacgtt
    21901 tttctgcgtt attaaattat cctattgttg ttagcaaaca agctgcaaaa aagagaatat
    21961 ctggggagtt tgatttatct aatccagaag aaatgcttga aaaactgaca ttacttgtgg
    22021 gactcatatg gtataaagat ggaaatgcat tatacatata tgactctgga gaattaataa
    22081 gcaaagttat tttgctggag aatatatcac ttaattatct tattcagtat ctgaaggatg
    22141 ctaaccttta tgatcacaga tatcctataa gaggaaacat tagtgataaa acattctata
    22201 tatctggtcc tccggcattg gttgaactgg tagctaatac agcaactttg cttgataagc
    22261 aggtttcttc tattgggaca gataaagtta attttggggt tataaaatta aaaaatacat
    22321 ttgttagtga cagaacttat aatatgagag gggaggatat tgtcatccca ggggtggcta
    22381 ctgttgtaga acgtttatta aataatggta aggctctgag taacagacaa gcccaaaatg
    22441 atccgatgcc accctttaat attacacaga aagtatctga ggatagtaat gacttttcat
    22501 ttagttcagt taccaatagt tctatattag aggatgtatc acttattgca tatccagaaa
    22561 ccaattctat attagtcaaa ggtaatgatc agcaaataca gattattcgc gatattatca
    22621 ctcagttaga tatagccaaa cgacatatag aattatcatt atggataatc gatattgata
    22681 aatctgaatt aaataacctt ggtgttaatt ggcaaggaac ggcttcattt ggtgattcgt
    22741 ttggtgcgtc atttaatatg agctcatcag catcgataag cactttggac ggcaataagt
    22801 ttattgcttc agttatggca ttaaatcaga aaaagaaagc aaatgtagtt tcaaggccgg
    22861 tgattttgac acaagagaat attccggcta tatttgataa taacaggact ttttatgtgt
    22921 cattagttgg tgagcgcaat tcttcgttgg agcatgttac gtatggaaca ttgataaatg
    22981 ttatacctcg cttttcatct cggggacaaa ttgagatgtc tttaacgata gaggatggaa
    23041 caggaaatag tcagtcaaat tacaattata ataatgaaaa tacatctgtc cttccagaag
    23101 ttggaaggac caaaatcagt acaatagcga gagttccaca aggcaaaagt ttgttgatag
    23161 gtggttatac gcatgaaacg aactctaacg aaataatatc tattcctttt ctctcctcta
    23221 ttcctgtaat cggtaatgtg ttcaagtata aaacaagtaa tatcagtaat attgtaaggg
    23281 tttttctgat tcaaccccgt gagataaagg agtcaagtta ttataatact gcagaatata
    23341 aaagcctcat atctgaaaga gaaatacaaa agactacaca aataatccca tctgaaacta
    23401 ctttgctgga agacgaaaaa tcattggttt catacttaaa ttactaacta taaagtaggt
    23461 gatgtatgct tgatgttaaa aatacaggag tttttagctc tgcattcatt gataagttga
    23521 atgcaatgac aaattcagat gatggagatg agactgctga tgcagagctt gattctggct
    23581 tggctaatag caagtatatt gactcatctg atgagatggc ttccgctctt tcgtcattta
    23641 taaacagaag agaccttgag aaactgaaag gaacaaatag tgatagtcag gaacgtattt
    23701 tagatgggga agaagatgaa attaatcaca agatttttga tttaaagaga acgttaaaag
    23761 ataaccttcc tctagatcgg gattttatag acagactaaa gagatatttt aaagatccaa
    23821 gtgatcaagt cttagcatta agggaacttt tgaatgaaaa agatcttact gctgaacaag
    23881 tcgaattatt aactaaaatt attaatgaga taatatcagg tagtgaaaaa agtgttaatg
    23941 ctggaataaa ttcagctata caggctaaat tatttggcaa caaaatgaaa cttgaaccac
    24001 agcttttgcg tgcatgttat cgtggtttta tcatggggaa catatcaaca acagatcagt
    24061 atatagaatg gcttggtaat tttggtttta atcacagaca tacaattgtg aattttgtag
    24121 agcagtcact gattgtagac atggattctg agaaaccgag ctgtaatgct tatgagtttg
    24181 gttttgtgtt atctaaatta attgcaatta agatgattag aacttcagac gtaattttta
    24241 tgaagaaact ggaatcctca agcttgctaa aagatggcag tttaagtgca gagcagctat
    24301 tgctaacttt attatatatt tttcaatatc caagtgaaag tgagcaaatt cttacttctg
    24361 ttatagaagt atcacgagcc agtcatgagg attctgtagt gtatcaaaca tatctatctt
    24421 ctgttaatga aagtcctcat gatatattta aaagtgaaag tgaaagagaa attgcgatca
    24481 atattctacg agagcttgtc acaagtgcat acaagaaaga gctttctaga taacaggaga
    24541 taaaagtgat ccagtctttt cttaagcaag taagtactaa gcctgaatta attatattag
    24601 tgcttatggt tatgatcatt gctatgttga tcataccatt acctacatac cttgttgatt
    24661 ttttaatagg gcttaatatt gtacttgcta ttctcgtttt tatgggatca ttttatattg
    24721 aaagaatatt gagtttttct acattccctt cggttttgtt gataacgaca ttatttcgtc
    24781 tcgctttatc aataagtact agtcgactaa ttttagttga tgcggatgcg ggtaaaatta
    24841 ttactacatt tggccagttt gtcattggtg atagtctggc tgtaggtttt gttatttttt
    24901 caatagtaac tgttgtgcag ttcattgtta taacaaaagg atctgaaagg gttgcggagg
    24961 tagctgctcg cttttctctt gacggtatgc cggggaaaca aatgagcatt gatgcggatt
    25021 taaaggctgg aattattgat gccgcaggag ctaaagaaag acgcagtatt ctggaacgtg
    25081 agagtcaatt gtatggttca tttgatggag caatgaagtt cattaaaggt gatgcaattg
    25141 ctggcatcat tattattttt gtcaatttaa taggaggtat ttctgttggt atgagtcaac
    25201 atggaatgtc cctatctggt gcattatcta cttataccat actaactatt ggtgacggat
    25261 tggtctctca gattcctgct ctgttaattt ctataagtgc aggatttatc gttacaagag
    25321 ttaatggtga tagcgataat atgggacgta acataatgtc ccagatattt gggaatcctt
    25381 ttgttctcat tgttacatca gctcttgcct tggcgatagg gatgttgcca ggttttccgt
    25441 tttttgtttt tttcctgata gcagttactt tgacggcttt attctattat aaaaaggtcg
    25501 tagaaaaaga aaaaagtctg tctgagtctg attctagtgg ctatactggt acatttgata
    25561 ttgataatag ccatgactca tctttggcaa tgatagaaaa tctggatgct attagttcag
    25621 aaaccgttcc tttaatttta ttatttgccg aaaataagat aaatgcaaat gatatggaag
    25681 gtcttattga aaggataaga agtcagttct ttattgatta tggtgtcagg cttccaacta
    25741 ttttatatag aacaagcaat gagcttaagg tcgatgatat tgttttgtta ataaatgagg
    25801 tgcgtgccga tagttttaat atatattttg acaaagtgtg tattacagat gaaaatggag
    25861 atatagatgc tctaggtatt cctgttgttt caacttcata taatgaacgt gttatttctt
    25921 gggtagacgt ttcatataca gaaaatctaa ctaatattga tgctaaaatt aaaagtgctc
    25981 aagatgaatt ttatcaccag ttgtcacaag ctttattaaa caacataaat gagatttttg
    26041 gtatacaaga aacaaaaaat atgttagatc agtttgaaaa tcggtatcct gatctattaa
    26101 aggaagtctt ccgacatgtg actatacaga gaatttctga ggtattacaa agattgcttg
    26161 gagaaaatat ttctgttcgc aatttaaaac ttattatgga gtctttggcg ctttgggctc
    26221 ccagagaaaa agatgtcata acattagttg aacatgtccg tgcatcactt tctaggtata
    26281 tttgtagtaa aatagctgtt tctggtgaga ttaaagttgt gatgctttcc ggatatattg
    26341 aggatgcaat aagaaaaggg ataaggcaaa cctctggtgg ctctttcttg aatatggata
    26401 tagaggtttc ggatgaggta atggaaactt tagcacatgc tttgagagaa ttgagaaatg
    26461 caaaaaaaaa tttcgttctt ttggtatcag tagatatacg taggtttgtt aaaagactta
    26521 tagataacag atttaagagt atactcgtta tatcgtatgc tgagattgat gaagcatata
    26581 ccattaatgt attaaagact atttagtgag gtttaaatat gagtaacatt aatttagttc
    26641 aattagttag agatagtctt ttcacgattg gttgtcctcc atcaattatt acagatttag
    26701 acagccattc agcgattacc atttctttgg atagtatgcc tgctattaat atagctcttg
    26761 tcaatgagca ggttatgtta tgggctaatt ttgatgcacc aagtgatgta aaacttcagt
    26821 catctgctta taacatatta aatttaatgt taatgaactt tagttatagc attaatgagc
    26881 tggtagagct tcatcgttct gatgagtact tacagttaag agttgttatt aaagatgact
    26941 acgttcacga tgggattgtt tttgcagaga ttctgcatga gttttatcag aggatggaaa
    27001 tcttaaatgg ggtcttataa tcaatgagct atacaaaatt gctcactcaa ttatcttttc
    27061 ctaatagaat ctcggggcca atcttggaaa caagtcttag cgatgtttcg attggtgaga
    27121 tttgtaacat tcaggctgga attgaaagta atgaaattgt tgcaagagct caggttgtag
    27181 gatttcatga tgaaaaaaca atattaagct tgattggaaa ttctcgtgga ctttcacggc
    27241 aaacgttgat taagcccact gcccagtttc ttcatacgca agtcggccgt ggattattgg
    27301 gagcagtagt caatccttta ggggaggtta ctgataagtt tgctgttaca gataacagtg
    27361 aaattcttta tcgacctgta gataatgctc ctccgctata tagtgaaagg gctgcaattg
    27421 agaagccttt tttaacaggt attaaggtta ttgattcttt actcacgtgt ggtgaaggac
    27481 agcgaatggg gatttttgcg tcagctggtt gtggcaaaac ttttctcatg aatatgctca
    27541 ttgaacatag tggtgctgat atatatgtta ttgggttaat tggtgagcga ggtcgagagg
    27601 ttactgaaac ggttgattat ttgaaaaact ctgagaaaaa aagcaggtgt gttttagtat
    27661 atgcaacttc ggattactct tcggttgatc gttgtaatgc tgcatatata gccactgcta
    27721 tagccgaatt ttttaggact gaaggacata aagtagcgct ttttattgat tcattaacaa
    27781 ggtatgccag agcattacgt gatgtggcct tagccgctgg agaatcacct gccagaagag
    27841 gctatccggt ttcggttttt gatagcttac ccagacttct tgaaaggcca ggaaagttaa
    27901 aggcaggtgg ctctattact gcattttaca ctgttctttt ggaggatgat gattttgctg
    27961 atccattagc tgaagaggta agatccattt tagatggaca tatatatttg agcagaaatc
    28021 tagcccaaaa aggacaattt cctgcaattg attccttaaa aagtataagc agggtattta
    28081 cacaggttgt tgatgaaaaa catcgtatta tggccgctgc atttcgggag ctactttctg
    28141 aaatagaaga gctaaggaca attattgact ttggtgaata caaaccgggg gagaatgcct
    28201 ctcaggataa aatctacaac aaaatatctg ttgttgagag ttttctgaag caagattatc
    28261 gactcggttt tacttatgag cagacaatgg agcttattgg tgaaacaatt agataaggtt
    28321 ttaaaaatca aagataaata tcaacggtct gttaaattaa ttgaagcaca catattaaca
    28381 ctaaaaaaaa aactctttgt atcgagatgt ggaggcatta gataaaagga ttatatactt
    28441 cctgcaactg gagaatgact tagaacctgt aggcgctcag agtgttagtc agcttttcaa
    28501 tacaagacgt aagatcgcta tagtaaaaaa gcatattatc caatatcagt ctgaacgaat
    28561 tcttctaaag ggaagaattg aagaaattca aaaagatatt gatgaggcaa atgcttctaa
    28621 aagaaaactg cttcataaag agagtaaaat atgtaaacgg attggcttga ttaaaagaaa
    28681 taactttgcc aaacagttga tacttgatga gttatcacaa gaggatatga agtatggcat
    28741 tagataatat aaacctaaat tttagtagtg acaaacagat agaaaaatgt gagaaactat
    28801 cttcaataga taatattgat agtctcgttt tgaagaagaa gaggaaggtt gaaattcctg
    28861 agtactcttt aattgcatct aactatttta ctattgataa gcactttgaa cataagcatg
    28921 ataaaggaga aatttatagt ggcattaaaa atgcgttcga acttagaaac gaacgagcga
    28981 catattctga tattccagaa tcaatggcca ttaaagaaaa tattttgata ccagatcaag
    29041 atatcaaagc aagggaaaaa ataaatatcg gcgatatgag ggggatcttt tcatataata
    29101 agagtggaaa tgcagacaag aacttcgaaa gaagtcatac ttcttctgta aaccctgata
    29161 atctgctaga atctgataat agaaatggtc aaattggttt aaaaaatcat agcttgtcta
    29221 ttgataagaa tattgctgac atcatttctt tactaaatgg aagtgttgct aaatcatttg
    29281 agctgcctgt aatgaataaa aatactgcag acataacccc atccatgtca ttgcaagaaa
    29341 aatcaatagt tgaaaatgat aaaaatgttt ttcaaaaaaa tagtgaaatg acttaccact
    29401 ttaaacagtg gggggctgga cattctgtta gtatttcagt ggagtctggt tcttttgttc
    29461 taaaaccgtc agatcaattt gtaggaaata aacttgactt aattttgaaa caagatgctg
    29521 agggtaatta cagatttgat agcagtcaac ataataaggg gaataaaaat aatagtacag
    29581 gatataatga acagagtgaa gaagaatgct aagaattaaa cattttgacg ctaacgaaaa
    29641 actacagatt ttatatgcaa agcaactctg cgagcgtttt tcgattcaga cattcaaaaa
    29701 taaatttaca ggcagtgaaa gtttagtcac tcttacttcc gtgtgtgggg attgggtaat
    29761 tcgtattgat acattatctt ttttgaaaaa aaaatacgag gtattttcag gattttctac
    29821 acaagaatct ttactgcatt tatcaaaatg tgtctttata gagtcgtcat ctgtattttc
    29881 gattccagaa ctgtctgata agattacttt ccggatcacg aatgaaatcc agtatgcaac
    29941 tactgggagt catttatgct gtttttcctc ttctttaggt attatttatt ttgacaagat
    30001 gccggtatta cgtaatcaag tttctcttga ctcattgcat catcttttag agttttgctt
    30061 aggttcatct aatgtaaggc tggctacttt aaaaagaatt cgcactggtg atataatcat
    30121 agttcagaaa ctttataatt tattattgtg taatcaagtt attattgggg attatattgt
    30181 gaatgataat aatgaggcaa aaattaatct gtcagaaagt aatggtgagt cagaacacac
    30241 agaagtttct ttggcattat tcaattatga tgatatcaat gtaaaagtgg actttattct
    30301 tttagaaaaa aatatgacaa tcaatgaact aaaaatgtat gtagaaaacg aattattcaa
    30361 gtttcccgat gacatagtta aacatgtaaa tattaaagta aatggttctt tggttgggca
    30421 tggggaactt gtttctattg aggatggtta tggtatcgag attagttctt ggatggtaaa
    30481 ggagtaatct catgctgagt gacatgtccc tcatcgctac actttctttt tttacgctat
    30541 taccattttt agttgctgct ggtacctgtt atataaagtt ttctattgtt tttgtaatgg
    30601 tacgaaatgc acttggtttg cagcaggttc catcaaatat gactctcaat ggtattgcat
    30661 tgattatggc tttgttcgtg atgaaaccca ttattgaagc tggttatgaa aattatctaa
    30721 atggccctca aaaatttgat acaatatctg atattgtccg tttttctgat tctggcttga
    30781 tggaatataa gcaatacttg aaaaagcata cagatcttga gttagcacgt tttttccaaa
    30841 ggtctgaaga agaaaatgct gatttaaaga gtgcagagaa taatgattat tcattgtttt
    30901 ccctcttgcc tgcatatgcg ctaagtgaaa tcaaagatgc gttcaaaata ggtttttatt
    30961 tatatttgcc atttgttgtt gttgatcttg ttatttcaag cattttactt gcacttggta
    31021 tgatgatgat gagtccaatc acaatatcag taccaataaa acttgtatta tttgttgcgc
    31081 ttgatgggtg gggtatcctg tctaaggcct tgattgaaca atatatcaat attcctgctt
    31141 agatgatgga ggtaatgtgt ctgatatagt ttatatgggt aataaggctc tttatttaat
    31201 ccttatcttt tccttatggc cagtaggtat agctacggtt attggattat ctattggttt
    31261 gttacagaca gtgactcaac tccaagagca gacacttcct tttggtataa agcttatagg
    31321 tgtctcaata tctttgctac ttctttctgg atggtatggt gaggttttat tgtctttttg
    31381 tcatgaaata atgtttttaa ttaagagtgg ggtttgatgg acatttcaag ctggttcgaa
    31441 agtattcatg tgtttttaat actcctgaac ggcgtttttt ttagattggc tccattattt
    31501 ttctttcttc catttttaaa taacggtata atttctccat ctattagaat acctgtgatt
    31561 tttcttgttg catcaggatt aattacttct ggtaaggtag acataggttc ttctgttttt
    31621 gaacatgttt atttccttat gttcaaggaa ataattgttg gcctccttct ctctttttgc
    31681 ttgtctcttc ccttttggat atttcatgct gttggtagca ttattgacaa ccagcgtggg
    31741 gcaacgctta gtagttcaat tgatcctgcc aatggtgttg atacgtctga gttggcaaaa
    31801 tttttcaatc ttttttctgc agttgtattt ctatacagtg gtggtatggt ctttatttta
    31861 gaatccatac aattgtctta taatatatgc ccgttatttt ctcaatgttc tttccgtgtc
    31921 tcaaatatct taacatttct gactttattg gcaagtcagg ctgttatttt agccagtcct
    31981 gttatgatag tattgttact atcagaagta ttacttggtg tattatcgag atttgctccg
    32041 cagatgaatg ctttttccgt atcattaact attaaaagtt tacttgcaat atttattatt
    32101 ttcatctgtt cttctactat ttacttttct aaagttcaat ttttcctcgg tgaacataaa
    32161 tttttcacaa atctatttgt tagataaaat attatggcaa ataaaacaga aaagccgaca
    32221 cctaaaaaac taaaggatgc cgcaaaaaaa ggacagtcat ttaaatttaa ggatttaacg
    32281 actgttgtta ttattctggt agggacattt actataatat cattcttttc cttaagtgat
    32341 gtaatgctct tatacagata tgtaataatt aatgacttcg aaattaatga gggtaaatac
    32401 ttttttgcag tggttattgt cttttttaag ataattggct tcccactttt tttctgtgtt
    32461 ctttcggctg tgttgccaac attggttcaa acaaagtttg ttcttgcgac taaagctatc
    32521 aagattgatt tttcagtatt aaaccctgtt aaagggttaa aaaaaatatt tagtataaag
    32581 acaataaaag aatttttcaa aagcattctg cttcttatta ttctagcatt aacaacctat
    32641 ttcttttgga ttaatgaccg aaaaataatt ttttctcagg tgttttctag tgttgatggc
    32701 ttatatctta tttgggggag gctgtttaag gatataatat tatttttctt ggcattttct
    32761 attcttgtta ttattcttga ctttgtgatt gagttcattt tatacatgaa agatatgatg
    32821 atggataaac aggagataaa aagagaatat atagagcaag agggacactt tgagacaaag
    32881 tcgagaaggc gtgagttgca tatcgagatt ctttcagagc agactaaatc tgatatacgt
    32941 aattcaaaat tagtggtaat gaacccgact catattgcaa ttggtattta ttttaatcca
    33001 gaaatagcgc ctgcaccttt tatttctctc attgaaacta accagtgtgc cttggctgtc
    33061 agaaaatatg caaatgaagt tggtataccg actgtgcgtg atgtgaaatt agctagaaaa
    33121 ctatataaaa cacatacaaa atatagtttt gttgattttg aacacttgga tgaagtccta
    33181 cgtcttattg tttggcttga gcaggttgaa aacactcatt aataagtaaa ggagatgtgt
    33241 tatatgggag ttaatttctg taataaaata ggtattgatc agtctgaatt tgaaatagaa
    33301 tcttccatca ttaactccat tgctaatgag gtattgaacc caatatcttt ccttagcaat
    33361 aaggatataa taaatgtttt actcagaaag atttcatctg aatgtgacct ggttagaaaa
    33421 gacatttatc gttgtgctct ggagttggtc gttgaaaaaa cacctgatga tttataatca
    33481 ttcataataa ctcttttgct gatataaata tttttgttga agatatttat gtagtcacag
    33541 gatcaattgt agaaaattgt tatgtttttg ttaggaactg cactaaataa tatatgataa
    33601 gacagcaaaa acgactgacc ataatccttc ttttattagg agttgataag agagactatt
    33661 catcctgtaa tgttaaaact ctgttatata gtattcgcga ttatgcaaag tctgtaaatg
    33721 atcatgagat actaaccgag agtaatcgtt tattatctca ttgcatttca gatagtaacg
    33781 gagctttctt taaaagtagt aaatacgttc cccttaagta cttacgtaaa aggagaatag
    33841 cacgaaagat accaaatgat tagcactttg ctagtaagct tgctggtaaa ctatattgac
    33901 cgttgggttt gttttgagac tgtaatttaa attgtgtaat tgcctgtttt tgatatgttt
    33961 actccaacaa cggagacagg taaattatgg acgaaaagaa gcttaaagcc cttgcggctg
    34021 aactggctaa aggcctcaaa actgaggccg acctcaatca gttttcccgt atgctgacga
    34081 aatatcccgg ctcttaaaac gatagtggct caggctttca tttttcagcg ttccccagaa
    34141 g
//
LOCUS       DAJTRD010000038        31446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTRD010000038 DAJTRD010000000
VERSION     DAJTRD010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.038"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            75..1352
                     /gene="waaA"
                     /locus_tag="OO315_001984"
     CDS             75..1352
                     /gene="waaA"
                     /locus_tag="OO315_001984"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5890909.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1360..1839
                     /gene="coaD"
                     /locus_tag="OO315_001985"
     CDS             1360..1839
                     /gene="coaD"
                     /locus_tag="OO315_001985"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5890910.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO315_001986"
     CDS             complement(1878..2687)
                     /gene="mutM"
                     /locus_tag="OO315_001986"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5890911.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO315_001987"
     CDS             complement(2785..2952)
                     /gene="rpmG"
                     /locus_tag="OO315_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5890912.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO315_001988"
     CDS             complement(2973..3209)
                     /gene="rpmB"
                     /locus_tag="OO315_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5890913.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO315_001989"
     CDS             complement(3426..4094)
                     /gene="radC"
                     /locus_tag="OO315_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5890914.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4266..5486
                     /gene="coaBC"
                     /locus_tag="OO315_001990"
     CDS             4266..5486
                     /gene="coaBC"
                     /locus_tag="OO315_001990"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5890915.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5464..5922
                     /gene="dut"
                     /locus_tag="OO315_001991"
     CDS             5464..5922
                     /gene="dut"
                     /locus_tag="OO315_001991"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5890916.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6029..6625
                     /gene="slmA"
                     /locus_tag="OO315_001992"
     CDS             6029..6625
                     /gene="slmA"
                     /locus_tag="OO315_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5890917.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO315_001993"
     CDS             complement(6662..7303)
                     /gene="pyrE"
                     /locus_tag="OO315_001993"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5890918.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO315_001994"
     CDS             complement(7369..8085)
                     /gene="rph"
                     /locus_tag="OO315_001994"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5890919.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8212..9075
                     /locus_tag="OO315_001995"
     CDS             8212..9075
                     /locus_tag="OO315_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5890920.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9296..10120
                     /gene="dinD"
                     /locus_tag="OO315_001996"
     CDS             9296..10120
                     /gene="dinD"
                     /locus_tag="OO315_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5890921.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10411..11028
                     /locus_tag="OO315_001997"
     CDS             10411..11028
                     /locus_tag="OO315_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5890922.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO315_001998"
     CDS             complement(11025..12707)
                     /gene="ligB"
                     /locus_tag="OO315_001998"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5890923.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            12965..13588
                     /gene="gmk"
                     /locus_tag="OO315_001999"
     CDS             12965..13588
                     /gene="gmk"
                     /locus_tag="OO315_001999"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5890924.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO315_002000"
     CDS             13643..13918
                     /gene="rpoZ"
                     /locus_tag="OO315_002000"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5890925.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            13937..16045
                     /gene="spoT"
                     /locus_tag="OO315_002001"
     CDS             13937..16045
                     /gene="spoT"
                     /locus_tag="OO315_002001"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5890926.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16052..16741
                     /gene="trmH"
                     /locus_tag="OO315_002002"
     CDS             16052..16741
                     /gene="trmH"
                     /locus_tag="OO315_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5890927.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            16747..18828
                     /gene="recG"
                     /locus_tag="OO315_002003"
     CDS             16747..18828
                     /gene="recG"
                     /locus_tag="OO315_002003"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5890928.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO315_002004"
     CDS             complement(18862..20067)
                     /gene="gltS"
                     /locus_tag="OO315_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5890929.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20347..21738
                     /gene="xanP"
                     /locus_tag="OO315_002005"
     CDS             20347..21738
                     /gene="xanP"
                     /locus_tag="OO315_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5890930.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            21859..23568
                     /locus_tag="OO315_002006"
     CDS             21859..23568
                     /locus_tag="OO315_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5890931.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO315_002007"
     CDS             complement(23621..25939)
                     /gene="yicI"
                     /locus_tag="OO315_002007"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5890932.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(25949..27332)
                     /locus_tag="OO315_002008"
                     /pseudo
     CDS             complement(25949..27332)
                     /locus_tag="OO315_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            27625..27719
                     /locus_tag="OO315_002009"
     tRNA            27625..27719
                     /locus_tag="OO315_002009"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27659..27661,aa:Sec,seq:tca)
     gene            27991..29202
                     /locus_tag="OO315_002010"
     CDS             27991..29202
                     /locus_tag="OO315_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5890933.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            29614..30657
                     /locus_tag="OO315_002011"
     CDS             29614..30657
                     /locus_tag="OO315_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5890934.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31172..>31446
                     /locus_tag="OO315_002012"
     CDS             31172..>31446
                     /locus_tag="OO315_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890935.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRLLQHGKDRSAGTPDIVT"
BASE COUNT         7882 a         8288 c         7800 g         7476 t
ORIGIN      
        1 aacgggaaaa gtaatggtaa agccacagct aaatacatag aatccccagc acatccataa
       61 gtcagctatt tactatgctc gaattgcttt acaccgccct tctctacctt attcagccgc
      121 tgatctggat acggctctgg gtgcgcggac gtaaggctcc ggcctatcga aaacgctggg
      181 gtgaacgtta cggtttttac cgccatccgc taaaaccagg cggcattatg ctgcactccg
      241 tctccgtcgg tgaaactctg gcggcgatcc cgttggtgcg cgcgctgcgt catcgttatc
      301 ctgatttacc gattaccgtt acaaccatga cgccaaccgg ttcggagcgc gtacaatcgg
      361 ctttcgggaa ggatgttcag cacgtttatc tgccgtatga cctgcccgat gcgctcaacc
      421 gtttcctgaa taaagtcgac cccaaactgg tgttgattat ggaaaccgaa ctatggccta
      481 acctgattgc ggcgctacat aaacgtaaaa ttccgctggt gatcgctaac gcgcgactct
      541 ctgcccgctc ggccgcaggt tatgccaaac tgggtaaatt cgtccgccgc ttgctgcgtc
      601 gtattacgct gattgctgcg caaaatgaag aagatggtgc acgttttgtg gcgctgggcg
      661 caaaaaataa ccaggtaacc gttaccggta gcctgaaatt cgatatttct gtaactccgc
      721 agttggctgc taaagccgtg acgctacgcc gccaatgggc accacaccgc ccggtatgga
      781 ttgccaccag cactcacgaa ggtgaagaga gcgtggtcat tgccgcacat caggcattgt
      841 tacagcaatt cccgaattta ttgctcatcc tggtaccccg tcatccagaa cgcttcccgg
      901 atgcgattaa ccttgtccgc caggctggac taagctatat cacacgctct tcaggggaag
      961 tcccctccac cagcacgcag gttgtggttg gcgatacgat gggcgagttg atgttactgt
     1021 acggcattgc cgatctcgcc tttgttggcg gttcactggt tgaacgtggt gggcataatc
     1081 cgctggaagc tgccgcacac gctattccgg tattgatggg gccgcatact tttaacttta
     1141 aagacatttg cgcgcggctg gagcaggcaa gcgggcttat taccgttacc gatgccacta
     1201 cgcttgcaaa agaggtttcc tctttactca ccgacgccgt ttaccgtagt ttctatggcc
     1261 gtcatgccgt tgaagtactg tatcaaaacc agggcgcgct acagcgtctg cttcaactgc
     1321 tggaacctta cctgccaccg aaaacgcatt gaggttgtta tgcaaaaacg ggcgatttat
     1381 ccgggtactt tcgatcccat taccaatggt catatcgata tcgtgacgcg cgccacgcag
     1441 atgttcgatc acgttattct ggcgattgcc gccagcccca gtaaaaaacc gatgtttacc
     1501 ctggaagagc gtgtggcact ggcacagcag gcaaccgcgc atctggggaa cgtggaagtg
     1561 gtcgggttta gtgatttgat ggcgaacttc gcccgtaatc aacacgctac ggtgctgatt
     1621 cgtggcctgc gtgcggtggc ggattttgaa tatgaaatgc agctggcgca tatgaatcgc
     1681 cacttaatgc cggaactgga aagtgtgttt ctgatgccgt cgaaagagtg gtcgtttatc
     1741 tcttcatcgt tggtgaaaga ggtggcgcgc catcagggcg atgtcaccca tttcctgccg
     1801 gagaatgtcc atcaggcgct gatggcaaaa ttagcgtagc gtttatgccg gatggtatgc
     1861 catccggctc gcatgaatta cttctggcac tgccgacaat aaaacgttgc ccgctgcgca
     1921 tgtttagtcg ccacaatcgg cgtaccgcac acccgacacg gctcaccttt tcgcccgtaa
     1981 acctgcaatt cctgcgcgaa atagcccggt ttaccatcac tttgcagaaa atctttcagc
     2041 gttgtaccac cctgctcaat cgaacgcagc aacaccgctt taatcacccg agctaacaat
     2101 tcacactctg ccagcgacag tgatgacgcc agccgatccg gatggatccc cgccgcaaac
     2161 agtgattcgc tggcatagat attccctacc cctaccacca gcttgttatc catcagccac
     2221 ggtttaatcg ccgttttttt cttcgcgcac ttctgatgca gatactcacc attgaaatcg
     2281 tcgctaagcg gctccggtcc aagatgggcc agcacattat gcccttccag ctctttggtc
     2341 cacagccagg caccaaagcg gcgcggatcg gtgtagcgca gcactttacc gttgctcatc
     2401 accaaatcca catggtcatg cttttcaggg ggaagttctt ctggaaggat gcgcaggctg
     2461 ccagacatcc ccaaatgaat gataatccag ccctcaggca gctccagcag cagatattta
     2521 gcccgccgct gcacgctaag cactggttga tcgcttaatc ggtagatctc ttctgaaacc
     2581 ggccagcgca agcgtccgtt gcgcaccacc gcatgaagaa tggttgcacc aacgagatgc
     2641 ggttctatgc cgcgacggct ggtttcaact tcgggtaatt caggcatagc atctccagga
     2701 atgaacagat gcaatcaata tgggggcaag cagataacaa aaaaccccgc cggagcgagg
     2761 tttttcgtta catcaaagcg agaattattt gattttcgct tctttgtaga tcacgtgctg
     2821 acgaacaact ggatcgaatt ttttcagttc cagtttttcc ggcttagtac gtttgttctt
     2881 cgtagtggta tagaagtgac cagtaccagc agaagaaacc agcttgattt tctcacgaat
     2941 acctttagcc atgatttatt tcctctaagt acttagtact tttcgccacg ggcacgcagt
     3001 tcagccagaa ctgtatcgat gcctttttta tcgattacac gcataccttt agcagatacg
     3061 cgcagggtga caaaacgctt ctcgctctca acccagaaac ggtgagagtg caggttcggc
     3121 aggaaacggc gtttagtcgc gttcagtgcg tgggaacggt tgttaccggt caccggacgc
     3181 ttgccagtaa cttggcagac tcgggacatg tctattctcc aaaaatcaaa ttagctcgag
     3241 cttcgtatgg ggtattggcg cctcgtcagg ctttacagcc cggtcatcgc agttctatgt
     3301 gaactctcga ttgccaggcc caaatgccaa acccgagatt ctcaaaggtg gcgtagtata
     3361 cgctgactca gcgatgtgct caagtcccga acagacaaag atcccgaagg atcgcgcata
     3421 gcgggttaaa tccagccgcg ttcggcaaaa gaaacatact ctccacgccc aatcacgata
     3481 tggtcgagca cgcgtaaatc catgaactga caactcttta ttatccgttc agtaatgagt
     3541 ttatccgctt tactgggttc agcacaaccc gaagggtgat tatgtgcaag gatcagcgcc
     3601 gaggcgttta tttttatcgc ttcgcggata atttcccgag gatggacttc aacatggttt
     3661 agcgtgccgg aaaaaagacg gctatgcgtt ataacccggt gttgtgagtc gagaaagatc
     3721 accataaaga tctcccgctc ctcacccgtg agctggcttt gtaaaaattc ccgcgtcatc
     3781 tccgggctga gtaaagggct ttcttcacgc atccgcacgt tgtagtaacg tcgcgccagt
     3841 tcagcaatcc cctttaactg ggcaaatttc gctacgccaa ttccatgaac accactaaat
     3901 tgttcatatt cagaggttaa caagccataa agagagccga aattctccag catctctttt
     3961 gccagggtta atacatcttt accgcgcgtt ccggtacgca gaaatagcgc cagcagctcg
     4021 acatccgtta aggcgctaat accaaacttc agcatttttt cgcgcggcat caacagctgt
     4081 gcattgtttt tcaccttcac ctcctttgtg gtgctcgcat cctgtcatag ccgttccgtt
     4141 aaatcgacgg ccagttttca atcctggaaa gagcctcgca aagtgaatct caggtgaagg
     4201 cacgacaaca aaagtattgt gataaaatcg ccaacttctg gtgtcaccca acaggaaaaa
     4261 tcatcatgag cctggccggt aaaaaaatcg ttctcggcgt tagcggcggt attgctgcct
     4321 ataaaacccc tgaactggtg cgtcgtttgc gcgatcgcgg ggccgacgtc cgcgtagcca
     4381 tgaccgaagc ggcaaaagcc tttatcaccc cacttagctt gcaggcggtt tctggttatc
     4441 ccgtttccga cagtctgctt gacccggcag ccgaagccgc tatgggccat attgagctgg
     4501 gtaaatgggc tgatttagtg attctcgccc ctgccacggc agatttgatt gcccgtgttg
     4561 ctgccggaat ggcgaatgac ctggtatcga cgatttgtct ggctacacct gcgcctgtag
     4621 ccgtgctccc cgccatgaac cagcagatgt accgtgccgc tgccacgcag cataatttag
     4681 aggtgcttgc ttcccgtggt ttgctcatct gggggccaga cagtggcagt caggcttgtg
     4741 gtgatatcgg tcctggacga atgctcgatc cgttaaccat tgtggatatg gcggtggcgc
     4801 atttttcgcc cgtcaacgac ctgaaacatc tgaacattat gattaccgcc ggtccgacgc
     4861 gtgaaccgct cgatccggtg cgttatatct ctaatcacag ctccggcaag atgggttttg
     4921 ctatcgccgc cgctgctgcc cgtcgtggcg cgaacgtcac gctggtatca ggtccagttt
     4981 cactaccgac gccaccgttt gttaaacgtg ttgatgtgat gaccgcgctg gaaatggaag
     5041 ccgccgtgaa tgcttctgta cagcagcaaa atatttttat cggttgcgcc gccgtggcgg
     5101 attatcgcgc agctaccgtg gccccagaaa aaatcaaaaa gcaggccacg cagggtgatg
     5161 aattaacaat aaaaatggtt aaaaaccccg atatcgtcgc aggcgttgcc gcactaaaag
     5221 accatcgacc ctacgtcgtt ggatttgccg ccgaaacaaa taatgtggaa gaatacgccc
     5281 ggcaaaaacg tatccgtaaa aaccttgatc tgatctgcgc gaacgatgtt tcccagccaa
     5341 ctcaaggatt taacagcgac aacaacgcat tacacctttt ctggcaggac ggagataaag
     5401 tcttaccgct tgagcgcaaa gagctccttg gccaattatt actcgacgag atcgtgaccc
     5461 gttatgatga aaaaaatcga cgttaagatt ctggacccgc gcgttaggaa ggaatttccg
     5521 ctcccgactt atgccacctc tggctctgcc ggacttgacc tgcgtgcctg tctcgacgac
     5581 gccgtagaac tggctccggg tgacactacg ctggttccga ccgggctggc gattcatatt
     5641 gccgatcctt cactggcggc aatgatgctg ccgcgctccg gattgggaca taagcacggt
     5701 atcgtgcttg gtaacctggt aggattgatc gattccgact atcagggcca gttgatgatt
     5761 tccgtgtgga accgtggtca ggacagcttc accattcaac ctggcgaacg catcgcccag
     5821 atgatttttg ttccggtagt acaggctgaa tttaatctgg tggaagattt cgacgccacc
     5881 gaccgcggtg aaggcggctt tggtcactct ggtcgtcagt aacacatacg catccgaata
     5941 acgtcataac atagccgcaa acatttcgtt tgcggtcata gcgtgggtgc cgcctggcaa
     6001 gtgcttattt tcaggggtat tttgtaacat ggcagaaaaa caaactgcga aaaggaaccg
     6061 tcgcgaggaa atacttcagt ctctggcgct gatgctggaa tccagcgatg gaagccaacg
     6121 tatcacgacg gcaaaactgg ccgcctctgt cggcgtttcc gaagcggcac tgtatcgcca
     6181 cttccccagt aagacccgca tgttcgatag cctgattgag tttatcgaag atagcctgat
     6241 tactcgcatc aacctgattc tgaaagatga gaaagacacc acagcgcgcc tgcgtctgat
     6301 tgtgttgctg cttctcggtt ttggtgagcg taatcctggc ctgacccgca tcctcactgg
     6361 tcatgcgcta atgtttgaac aggatcgcct gcaagggcgc atcaaccagc tgttcgagcg
     6421 tattgaagcg cagctgcgcc aggtattgcg tgaaaagaga atgcgtgagg gtgaaggtta
     6481 caccaccgat gaaaccctgc tggcaagcca gatcctggcc ttctgtgaag gtatgctgtc
     6541 acgttttgtc cgcagcgaat ttaaataccg cccgacggat gattttgacg cccgctggcc
     6601 gctaattgcc gcgcagttgc agtaatatga cgccggatga cttttcatcc ggcgagtttc
     6661 tttaaacgcc aaactcttcg cgataggcct taaccgccgc cagatgttcc gccatttccg
     6721 gcttctcttc cagataggca atcagatctt tcagggtgat gatagagatc actttgcagt
     6781 tgtaatcacg ctcaacttcc tgaatcgccg aaatctcgcc gcgcccgcgt tcctgacgat
     6841 cgagcgaaat caacacgcca gcaagcgtcg cgccattggc ctgaataatc tccatcgact
     6901 cgcgaatcgc cgttccggcg gtgatcacat catctaccag cattacgcgt ccttgtaacg
     6961 cgctaccaac cagattgccg ccttcaccgt ggtcttttgc ttctttgcgg ttaaagcagt
     7021 acggcaggtc caggtcgtga tgctccgcca gtgccacagc ggttgtggtg gcaatcggga
     7081 tccctttgta agcagggcca aacagcagat cgaactcaat gccggaatcc accaacgctt
     7141 cagcgtaaaa acggcctaac agtgccagat cgcgcccggt attaaacagc ccggcgttga
     7201 agaaataggg gcttttgcgc ccggatttca gcgtaaactc gccaaacttt aacacctgct
     7261 tgctaagcgc aaattcaata aactggcgct gatatgattt catgccttcg ctcctcatct
     7321 tacttttcta cagacaaaaa aaaggcgact catcagtcgc cttaaaaatc agtttgccag
     7381 cgccgccttc tgcgtcgcta caatggattc gattccccct cgggccagcg ccaacaaggt
     7441 gagtagctct tcatgggtga acggctcgcc ttctgccgtc ccctgcactt caatgatgcg
     7501 cccgtcttcg gtcatcacta cgttcatgtc ggtctctgcg gctgagtctt caacgtattc
     7561 cagatcgcaa attgcttcac cgtttacaat accgacagaa actgcggcta ccatcccttt
     7621 catcgggttg gttttcagct tgccgttttc caccagcttc tgtagcgcat ctgccagcgc
     7681 cacgcaggca cccgtaatcg acgcggtacg agtgccacca tcagcctgaa gcacgtcgca
     7741 gtccagcgta atggtgaact cacccagcgc tttcaaatct actgccgcgc gaagagcacg
     7801 ggcgatcaga cgctggattt ccattgtgcg tccaccctgc ttacctttcg ccgcttcacg
     7861 agcgttacgg gtgtgggtag aacgtggcag catgccgtac tctgcggtga tccagccctg
     7921 gccctgacct ttcaggaagc gcggcacgcc ttcttcaata gaggcggtac acaatacttt
     7981 ggtatcgcca aattcgacca gcaccgagcc ttctgcatgt tttgtatagt tacgagtcag
     8041 ggtaacggga cgcacctgat tattgctacg gcctgctgga cgcatattga aatctccggc
     8101 ttgaaacaaa tgtggctgcg cattatacgg acttccggcg gttattccta tcctgacaag
     8161 gcatcgatgg ctataatcct tccacctctc cttttataaa caggaacgtc tatgatccgc
     8221 agtatgaccg cctacgcccg gcgtgaaatc aagggtgaat gggggagcgc aacctgggaa
     8281 atgcgctcgg taaaccagcg ttatctggaa acttactttc gtctgccgga gcagttccgt
     8341 agccttgaac ctgtcgttcg cgagcgtatt cgttctcgcc tgacgcgcgg taaagtggaa
     8401 tgtaccctgc gctatgagcc agatgttagc gcacaaggtg agctgatcct caatgaaaaa
     8461 ctggctaaac agctggtaac tgccgcgaac tgggtaaaaa tgaagagtga cgaaggggaa
     8521 atcaacccgg ttgatattct acgctggccg ggcgtgatgg cagcccagga gcaggatctc
     8581 gacgcgattg ccgctgaaat tctcgcggcg ttggatggta cgctggacga ctttatcgtc
     8641 gcgcgcgaaa ccgaaggtca ggcactgaaa gcattgatcg agcagcgtct ggaaggcgtc
     8701 accgccgaag tggtcaaagt ccgcgcccat atgccggaaa ttctgcaatg gcagcgtgag
     8761 cgtctggtcg cgaagctgga agatgctcag gttcagttgg aaaacaaccg tctggagcag
     8821 gagttggttc tgctggcaca acgaattgac gttgccgaag agctggaccg cctcgaagcg
     8881 catgttaaag agacgtacaa cattctgaag aaaaaagaag cggttggtcg tcgtctggac
     8941 tttatgatgc aggagttcaa ccgtgagtcg aacactcttg cgtcgaaatc tatcaatgcc
     9001 gaagtgacaa actccgccat cgagctgaaa gtgttgattg agcagatgcg cgagcagatt
     9061 cagaatattg agtaactcgt aacaccaatt ctgacttttc agccttcaca aatgccgccc
     9121 actcaaacag agcggcattt ttcttccccg caacttcaat tctgttttgc gtgcctgctc
     9181 cagattttgc gatgtttttt tgcccagcac actgagaacg tgagatactc acaactgtat
     9241 ataaatacag ttacagattt actttctttg caatcgatat cacatggagt gggcaatgaa
     9301 cgaacatcat caaccttttg aagagataaa actgattaat gcaaacggag cagaacaatg
     9361 gtcagcaaga caacttggga aactactggg ttattcagag tatcgtcact ttatacctgt
     9421 attaacgcgc gccaaagagg cctgtgagaa cagtggtcac acaattgatg accatttcga
     9481 ggagatcctc gatatggtca aaattggctc aaatgccaaa agagcattaa aagacatcgt
     9541 actctcccgc tatgcctgtt acctggtagt acaaaacggc gaccccgcga aaccggtcat
     9601 tgcggcaggg cagacttatt ttactatcca gacccgacgg caggagcttg ctgacgatga
     9661 agcatttaag caacttcgtg aagacgaaaa gcgtctgttt ctgagaaatg agttgaaaga
     9721 acataacaaa caattggttg aggcagcaca gcaagctggg gtagcgacag ctactgattt
     9781 cgccatattt cagaatcatg gttaccaggg gctgtatggt ggattagatc agaaagccat
     9841 ccaccaactg aaggggctga aaaagagtca gaagatcctg gatcatatgg gttcaacaga
     9901 actggcggct aatctctttc gagctaccca aacagaagaa aaactcaagc gggatagcgt
     9961 gaattcaaaa cagcaagcta ataccacaca ctttgacgtg ggtagcaagg tgaggcaaac
    10021 cattcaggaa cttggcggaa ccatacctga ggagttgccg accccgcagg tcagcatcaa
    10081 gcagttggaa aacagtgtaa aaattacaga gaagaaatag cagaaacgaa aaactctttt
    10141 tgttgattga gacacccgat gcgtaagcca aggtccaggt gcagtgaaca caatggctaa
    10201 acattgcacc tttcttttcc cccctcagtt ttaacctatt ttttcttatg cattttctca
    10261 gacaagaagt cagaagaatg catctacgct acagaaaata gcgatttcac ataactaaaa
    10321 gttatcaatt tcctctcacc ttaaaaaata tcaatcgtga caatgcgcac aaatcgctac
    10381 cctgccagac agatttttag ggagagaacc atgctgttac acattttgta tttggttggc
    10441 attactgccg aagccatgac gggggcgctg gcggccgggc gacggcgcat ggatacattt
    10501 ggcgtaatta ttattgcaac cgccaccgca attggcggag ggtcagtgcg cgatattctg
    10561 ctgggccact atccgctcgg ctgggtcaaa cacccggaat atgtgattat cgtcgctacc
    10621 gccgcagtac tcaccactat cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg
    10681 gtgctcgatg cgctcggact ggtggtcttt tctatcatcg gcgcacaggt tgcactggat
    10741 atggggcacg gcccaattat tgccgttgtc gcggcggtaa ccaccggtgt atttggcggc
    10801 gttttgcgcg atatgttctg taaacgcatt ccgctggtct ttcaaaaaga gttgtatgcc
    10861 ggtgtctcat ttgcctccgc cgtgctgtac atcgcgctgc aacactatgt ttctaaccat
    10921 gatgtggtga ttatctccac cctggtattc ggcttttttg ctcgtttact ggcgctacgt
    10981 cttaagctgg gattaccggt cttttactac agccacgaag gtcactaagg ttcaaaacct
    11041 gtgatctgct gggcagccag ccaactgccc agcttcttga tttgcgcatt ttccttccat
    11101 tcaataacct gtctggcgcg cccagatcca gtccccggca gctgctgcca gaactgctcc
    11161 gtgctaaata aaagttgcga ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt
    11221 agcggtattc ccattgccat cacccagcga gtaaaaggct ggttacgagc cagattaaac
    11281 tgatgccata actgcgcact tttacttttc gcgatccccg gcgtgttctg taattgctct
    11341 ggcgttaata aaagccagga aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc
    11401 caaccggcct caccaatgcc atccagcccc agaacctgtt ttgaccccag ccagactaag
    11461 cgtgaaatga actgttcctg acaaacatca gaagcaaagt agcaggtcaa cgggttaaag
    11521 cggttttctg gtggtgtcgg ttttgtacgt tctgcaccgc gccagaccac atcatcaatg
    11581 cgaggaatac cctgaccggc aaggctgacg aggatctgat caccaggcgc aatatcccac
    11641 tcctgccagc gcctgacgga accaatattc acccgctgga cttttttatc atccagcatg
    11701 acaggtgcga gtgacgcaac caccgatatt ttaccgctct tacccaccgc aaactgaatc
    11761 gccttcactt cggcaacctg agctacaggt tgatatttcc aggccaccag ccactccgcc
    11821 tggcccggta gccaatgacg ggattcgggt tccttagccc ctcgcacaac cacgccatcg
    11881 gtgacgaagg gtaatttcgc tttccaccac tcattgcgta cgcgcgcaac ctcatcagca
    11941 tttttaaccg cacgggtata cctctgcgtt agagtaaaac ctgcggtagc cagctccttt
    12001 aaacgatcgg tcattaactg cggtccatcc ggccatgccc agacaaaaac agccagtgag
    12061 tttagcgtgt cgctattccc ctggcgcatc atcaggccag caacttttgc gcgggcattt
    12121 attcccccca tttgttgttg gatatgcccc tcgcgctgga gaaatatttc cccctgaagc
    12181 gtactgttgg ctaaaggccc gctaacggtt tgcagcacag cggaaattaa gctaactttc
    12241 tgcgtccagt cctcgccttt caggccatta ccgcgactga ttgctttgtt cagtttcccg
    12301 tcccgataaa ccagggttac cgcaacgcca tcaacttttg gctgcaccca aagatcgcta
    12361 cgttctcgca tccacagact taatgcgttt ttatccgcca ttttgcgtac acccgtatgg
    12421 gcaaccggat gcataaccgc accatttaaa ggcggcatca tcacgtcgcg gggctcgctc
    12481 acaaagcagc gttgccactg cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc
    12541 acctcacttt ttccttcctt ccagtagtca tcgtcccact gttttatttg ctgttgcagg
    12601 cgggaaattt cttcctgtgc tctggctggc gaccaggccg gacagaccgc ccacacagat
    12661 gattgccagc acaagatact tattaatatc gccatccata ctttcatctt cacctccgct
    12721 gtagtcaggc tggcagatat acaacgtggt gaaggcaaag ccgagtggca aaaacggagt
    12781 ctgcgaggac gcttcctgag aatcgtcttt attgcagtaa atcacaggca aatgcggaaa
    12841 cagctacgca aaacgcaaca actttgcgca aaaagtgtga gcaagggcta cgtcacatgg
    12901 ccgcgccgtg tataataagc tcgtatgtag gctttatttc gctaatcaca tacgaaagat
    12961 actcatggct caaggcacgc tttatattgt ttctgccccc agtggcgcgg gtaaatccag
    13021 cctgattcag gctttattaa aaacccaacc gttgtatgac acccaggttt ctgtttcaca
    13081 caccacacgc caaccgcgtc ctggtgaagt ccacggtgaa cattatttct ttgttaatca
    13141 tgatgaattt aaagaaatga ttagcagaga tgcgttcctc gaacacgcag aagtttttgg
    13201 taattactat ggcacttcgc gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt
    13261 ttttctcgat atcgactggc agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg
    13321 gagtatcttt attttaccgc cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg
    13381 tcaggacagc gaagaggtca ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca
    13441 ttacgccgaa tatgattatc tgattgtgaa tgatgacttc gataccgcgt tgaccgattt
    13501 gaagaccatt attcgcgccg aacgtctgcg catgagccgc caaaagcagc gtcatgacgc
    13561 tttaatcagc aaattgttgg cagactgaac ctgatttcag tatcatgccc agtcatttct
    13621 tcacctgtgg agctttttaa gtatggcacg cgtaactgtt caggacgctg tagagaaaat
    13681 tggtaaccgt tttgacctgg tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg
    13741 cggaaaggat ccgctggtac cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga
    13801 aatcgaagaa ggtctgatca acaaccagat cctcgacgtt cgcgaacgcc aggaacagca
    13861 agagctggaa gccgctgaat tacaagccgt taccgctatt gctgaaggtc gtcgttaatc
    13921 acaaagcggg tcgcccttgt atctgtttga aagcctgaat caactgattc aaacctacct
    13981 gccggaagac caaatcaagc gtctgcggca ggcgtatctc gttgcacgtg atgctcacga
    14041 ggggcaaaca cgttcaagcg gtgaacccta tatcacgcac ccggtagcgg ttgcctgcat
    14101 tctggccgag atgaaactcg actatgaaac gctgatggcg gcgctgctgc atgacgtgat
    14161 tgaagatact cccgccacct accaggatat ggaacagctt tttggtaaaa gcgtcgccga
    14221 gctggtagag ggggtgtcga aacttgataa actcaagttc cgcgataaga aagaggcgca
    14281 ggccgaaaac tttcgcaaga tgattatggc gatggtgcag gatatccgcg tcatcctcat
    14341 caaacttgcc gaccgtaccc acaacatgcg cacgctgggc tcacttcgcc cggacaaacg
    14401 tcgccgcatc gcccgtgaaa ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat
    14461 ccaccacatt aaaaccgaac tcgaagagct gggttttgag gcgctgtatc ccaaccgtta
    14521 tcgcgtaatc aaagaagtgg tgaaagccgc gcgcggcaac cgtaaagaga tgatccaaaa
    14581 aatcctctcg gaaatcgaag ggcgtttgca ggaagcggga attccctgcc gcgtcagtgg
    14641 tcgcgagaag catctttatt cgatttactg caaaatggtg ctcaaagagc agcgttttca
    14701 ctcaatcatg gacatctacg ctttccgcgt gatcgtcaat gattccgaca cctgttatcg
    14761 cgtgctcggg caaatgcata gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat
    14821 cgccattcca aaagcgaacg gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg
    14881 tgtgccggtt gaggtccaga tccgtaccga agatatggat cagatggcgg agatgggtgt
    14941 tgccgcgcac tgggcttata aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc
    15001 ccagcgctgg atgcaaagcc tgctggagct gcaacagagc gccggtagtt cgtttgaatt
    15061 tatcgagagc gttaaatccg atctcttccc ggatgagatt tacgttttca caccggaagg
    15121 gcgaattgtc gagctgcctg ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac
    15181 cgatatcggt catgcctgcg tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca
    15241 gccgcttacc agcggtcaaa ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc
    15301 cgcgtggctg aactttgtcg ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa
    15361 aaacctcaag cgtgatgatt ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg
    15421 tggtagccgt aagctgaatg aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat
    15481 gaagctggca acgcttgacg atctgctggc agaaatcgga cttggtaacg caatgagcgt
    15541 ggtggtcgcg aaaaatctgc aacatgggga cgcctccatt ccaccggcaa cccaaagcca
    15601 cggacatctg cccattaaag gtgccgatgg cgtactgatc acctttgcga aatgctgccg
    15661 ccctattcct ggcgacccga ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca
    15721 ccatgaatcc tgccgtaata tccgtggcta ccagaaagag ccagagaagt ttatggctgt
    15781 ggaatgggat aaagagacgg cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa
    15841 tcatcagggc gcgctggcaa acctgacggc ggcaattaac accacgactt cgaatattca
    15901 aagtttgaat acggaagaga aagatggtcg cgtctacagc gcctttattc gtctgaccgc
    15961 tcgtgaccgt gtgcatctgg cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat
    16021 taaagtcacc cgaaaccgaa attaatgttt tatgaaccca acacgttatg cacgcatctg
    16081 cgaaatgctc gccaggcggc agcctgatct gaccgtctgc atggagcagg tccacaaacc
    16141 tcataacgtt tctgcgatta ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc
    16201 cgtctggcct ggtagccgca tgcgcaccat ggcttcggca gcggcgggta gtaacagctg
    16261 ggtacaggtg aaaacacacc gcaccattgg cgatgccgtc gctcatctca aaggccaggg
    16321 catgcagatt ctggcaaccc atctttctga taatgctgtc gatttccgcg aaattgatta
    16381 cactcgcccg acctgcattt tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt
    16441 ggccctggcg gatcaggaca tcatcattcc gatgatcggc atggtgcagt cgctgaatgt
    16501 ttccgttgcc tcagccctca ttctttacga agcccagcgt cagcggcaaa atgcaggcat
    16561 gtacctgcgt gaaaacagca tgttgccgga agcagagcaa caacgcctgt tgtttgaagg
    16621 cggctatccg gtgctggcga aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa
    16681 tcagcaaggc gagatcgaag ctgatgccga ctggtgggct actatgcagg ctgcagggta
    16741 agtgccatga aaggtcgcct gttagatgct gtcccactca gttccctaac gggcgttggc
    16801 gcagcactta gtaacaaact ggcgaaaatc aacctgcata ccgtgcagga tctactctta
    16861 caccttcccc tgcgctacga agatcgcacc catctctacc ccatcggaga actactgccg
    16921 ggcgtttatg ccacggtgga aggcgaagtg ctgaactgca atatctcctt cggcggtcgg
    16981 cggatgatga cctgccagat cagcgacggt tccggcatcc tcaccatgcg ctttttcaat
    17041 ttcagcgcgg caatgaaaaa tagcctggcg acgggccgcc gtgtactggc ttatggcgaa
    17101 gcaaagcgcg gtaaatatgg tgcggagatg atccacccgg aataccgcgt gcagggcgat
    17161 ctcagcacgc cagaattaca ggaaacgctc acgccggttt atccaacaac ggaaggcgta
    17221 aagcaggcca cgctgcgtaa attaaccgac caggcgctgg atctgctcga cacctgcgcc
    17281 attgaagaac tcctgccgcc ggaactgtca caaggaatga tgacgctacc ggaagcgttg
    17341 cgcactttgc accgcccgcc accgacgcta cagcttagcg atctggaaac cgggcagcat
    17401 ccggcgcaac gtcgtctgat tctggaagaa ctgctggcgc acaacctcag catgttagcc
    17461 ttacgtgccg gagcacagcg ttttcatgcc cagccgctga gcgccaatga cacgctgaaa
    17521 aataaactcc tcgccgcctt accgttcaag ccaacgggcg cacaggcacg cgtagtggcg
    17581 gagatcgagc atgatatggc gctggatgtg ccgatgatgc gcctggtaca gggcgatgta
    17641 ggttccggta aaacgctggt cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa
    17701 caggtggcac tgatggcacc aaccgaatta ctcgccgagc agcacgccaa taacttccgc
    17761 aactggtttg caccgctcgg tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa
    17821 gcgcgactgt ctcagcagga agccatcgcc agcggtcagg tgcagatgat tgtcggtaca
    17881 cacgccatct tccaggaaca ggtgcagttt aacggcctgg cgctggtgat tatcgacgaa
    17941 cagcatcgtt ttggcgtgca tcagcgtctg gcattgtggg agaaaggcca gcagcagggc
    18001 ttccatccgc atcagttgat catgaccgcc acgccgatcc cccgcacgct ggcaatgacg
    18061 gcgtatgccg atctcgatac ctcggtgata gatgagctgc cgccaggccg cacgccagtg
    18121 actacggtcg ctattcctga tacccgccgt accgacatca ttgaccgcgt gcgccacgcc
    18181 tgcataactg aaggtcgtca ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta
    18241 ctggaagcgc aggcggcgga agctacctgg gaagagttga aactggcgct accagaattg
    18301 aatgttggcc tggtacacgg gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg
    18361 tttaaacaag gtgagctaca tctgctggtt gccactaccg ttattgaagt cggtgttgat
    18421 gtgcctaacg ccagcctgat gattatcgaa aaccctgagc gtctgggtct ggcgcagtta
    18481 caccagctgc gcgggcgcgt aggtcgtggc gcggtggctt ctcactgcgt gctgctctac
    18541 aaaacgccgc tttctaaaac ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac
    18601 ggttttgtga ttgcgcaaaa agatctggag attcgcggcc ctggcgaatt gttaggcacg
    18661 cgtcagacgg gtaatgctga atttaaagtg gcggatttac tgcgcgatca ggcgatgatc
    18721 ccggaagttc agcgcctggc gcgacatatt cacgaacgtt acccacaaca ggcaaaagcc
    18781 ttgatagaac gctggatgcc ggagacagaa cgttactcga atgcgtaaaa ggcggcggtg
    18841 agaagaccgc cgcttcatcg gttaaccggc aaaaatcggc aacattaaat acagcttaat
    18901 caccagcgca ttgacgatat cgataaagaa cgcaccgacc atcggcacca ccaaaaacgc
    18961 catgtgcgac gggccaaagc gttcagtgat cgcctgcatg ttggcgattg ccgttggcgt
    19021 tgcaccgagg ccaaaaccac agtgacccgc agccagcact gccgcatcgt agtttttgcc
    19081 catcatgcgc caggtaacga agatggcata caacgccatg aagatggtct gtaccaccag
    19141 aatcgccagc atcggcagcg ccagcgaagc cagctcccac agtttcagcc ccatcaacgc
    19201 catcgccagg aacaacgaca agcttacgtt acccagcacg gataccgcac gctcaaagac
    19261 gcggtaaaag cccattattg acagaccgtt gctcagaatc acgccaacaa acagtacaca
    19321 gacgaaggtc ggcagttcaa aagcagtgcc agccaaaagt tgcgcaacaa ttttccccac
    19381 cgtcaggcag atagcaatca gcgcgatagt ttcaatcagc accaacgagg tgatcatgcg
    19441 tcccacatcc ggcttttcaa acgccgtcgg gacttcctgg tcatccggaa taccgttcgg
    19501 cgtggtggag tgtttcacca gatagcgcgc caccggaccg ccaatcaagc cgcccagcac
    19561 cagaccgaac gttgcacagg ccatcgccac ttccgtcgca ttggtgaagc cataacgttc
    19621 aatgaacaat ttactccacg cagcgcccgt accgtgaccg ccggaaagcg taatagaacc
    19681 ggccaacagc cccatcagcg gatcaagccc taacaggcta gccataccaa tgccaatggc
    19741 attttgcatc accaacagac caacaaccac aatcaagaag atgccaacca cacgcccacc
    19801 ggcacgcaaa ctggcaatgt tggcgttcag gccaatggtg gcgaagaaag ccagcattaa
    19861 cggatcgcgc agggacatat caaagttgac ttcccagccc atgctttttt tcagtactag
    19921 tagcgccagc gccaccaaca aaccacccgc aacaggttcc ggtatggtgt atttcttcaa
    19981 aaaggagacg gaatggacca acttacgccc gagcagcaac gtcagcgttg cggcaacaag
    20041 cgttgctaaa gtatcgagat gaaacatagt tactcctttg tatccgcatc actctttcat
    20101 actcgtcata cttcatcgcg cagtggcaac ttcatgaact atgacgggta tcgagttatg
    20161 ctttcctggg tcaaattctc agctgttaat gaacaaatgg catggatttt aagcagaaaa
    20221 ataccaaaag ttatataaaa aagtgcattt atctcattta aatatttaga tgaggatgtg
    20281 gcaaacgttt gcttttctct ttcggtcagc taaaatgccc gctttgctac cacgggattg
    20341 ttttcgatgt ctgtttccac cctcgagtca gaaaatgcgc aaccggttgc tcagactcaa
    20401 aacagcgaac tgatttaccg tcttgaagat cgtccgccgc ttcctcaaac cctgtttgcc
    20461 gcctgtcagc atctgctggc gatgttcgtt gcggtgatca cgccagcgct attaatctgc
    20521 caggcgctgg gtttaccggc acaagacacg caacacatta ttagtatgtc gctgtttgcc
    20581 tccggtgtgg catcgattat tcaaattaag gcctggggtc cggttggctc cgggctgttg
    20641 tctattcagg gcaccagctt caactttgtt gccccgctga ttatgggcgg taccgcgctg
    20701 aaaaccggtg gtgctgatgt tcctaccatg atggcggctt tgttcggcac gttgatgctg
    20761 gcaagttgca ccgagatggt gatctcccgc gttctgcatc tggcgcgccg cattattacg
    20821 ccgctggttt ctggcgttgt ggtgatgatt atcggcctgt cgctaattca ggtggggctg
    20881 acctccattg gcggcggtta cgcagccatg agcgataaca ccttcggcgc accgaaaaat
    20941 ctgctgctgg caggcgtggt tttagcctta attatcctgc ttaaccgtca acgtaaccct
    21001 tacttacgcg tggcctcact ggtaattgcg atggcggccg gatatgcgct ggcgtggttt
    21061 atgggcatgt tgccagaaag caacgaaccg atgacgcaag aactgattat ggtgccaacg
    21121 ccgctctatt acggtcttgg cattgaatgg agtctgctgc tgccgctgat gctggtcttt
    21181 atgatcactt cgctggaaac cattggcgat atcacggcga cctctgacgt ttccgaacag
    21241 ccagtgtccg gtccgctgta catgaaacgc ctgaaaggcg gcgtgctggc aaacggcctg
    21301 aactcgtttg tttcggcggt gtttaacacc ttcccgaact cctgcttcgg gcaaaacaac
    21361 ggagtgatcc agttgactgg tgttgccagc cgctatgtcg gttttgtcgt cgcgctgatg
    21421 ttgatcgtgc tgggtctgtt cccggcagtg agcggttttg tacaacacat tccagaaccg
    21481 gttctgggcg gcgcaacgct tgtaatgttt ggcaccatcg ccgcctccgg tgtgcgtatc
    21541 gtttctcgtg agccgctgaa ccgtcgggcg attctgatta tcgcgctgtc gctggcggtt
    21601 ggtctgggcg tgtctcagca gccgctgatt ttgcagtttg cccctgaatg gctgaaaaac
    21661 ctgctctcct ccgggatcgc cgcgggcggt attactgcca tcgtgctgaa tctgattttc
    21721 ccaccagaaa aacagtaatt cactggcggc ggtaagataa ttaccgccgc agtctcacct
    21781 ctttcaccat tcccgccttg aggattgcgc gtaaatcgtg cataactccc ttatgtgcat
    21841 ttcacgggat ggaagaccat gaaatttatt gggaagctgc ttctctacat tctcatcgct
    21901 ctgttagtgg tgatcgctgg cctctatttt cttctgcaaa cccgctgggg agcagaacat
    21961 atcagcgcat gggtttccga gaatagcgac tatcatctgg ccttcggggc gatggatcac
    22021 cgtttttccg cgccatctca tatcgtgctg gagaatgtca cgtttggtcg tgatggtcag
    22081 cccgcgcccc tggtggcaaa aagtgtcgac attgcgctaa gcagtcgcca actgaccgaa
    22141 ccacgccatg tcgataccat cctgctggaa aacgggacgc tgaatctcac cgaccagacc
    22201 gcgccgctac cgttcaaagc cgatcgtctg caactgcgtg atatggcgtt taatagcccg
    22261 aatagcgaat ggaaactgag cgcgcagcgg gtaaatggcg gcgtggttcc gtggtcacca
    22321 gaagccggta aagtgctggg tacgaaggcg cagattcagt ttagtgccgg atcgctttcg
    22381 ctcaatgatg ttcctgccac caatgtactg attgaaggca gtattgataa cgatcgcgtt
    22441 acgctgacta acctgggtgc cgacatcgcc cgcgggacat taaccggaaa cgcgcagcgt
    22501 aacgccgacg gcagctggca agtggaaaat ctgcgcatgg cggatatccg tctacaaagc
    22561 gaaaaatcgc taaccgactt ctttgcgcca ttacgctctg tcccgtcgtt gcagattggt
    22621 cgcttggaag tcatcgatgc tcgtctgcaa ggtccggact gggcggtgac cgacctcgat
    22681 ctcagcttgc gcaacatgac cttcagtaaa gatgactggc agacacagga aggcaaactg
    22741 tcgatgaacg ccagcgagtt catttatggt tcgctgcatt tatttgaccc gattataaac
    22801 gcggaatttt ccccgcaggg cgtagcgctg cgccagttca ccagccgctg ggaagggggt
    22861 atggtcagaa cgtcagggaa ctggctgcgt gacggtaaaa cgttgatcct tgatgatgcg
    22921 gcaattgccg ggctggaata taccttgccg aaaaactggc aacagttgtg gatggaaacg
    22981 acacccggtt ggttaaacag cctgcaactg aagagattta gcgccagccg caatctgatc
    23041 attgatatcg accctgactt cccgtggcag ctcaccgcgc tcgatggtta cggtgccaac
    23101 ctgacgctgg ttaccgatca taaatggggc gtctggagtg gctcggcgaa tctgaatgcc
    23161 gccgccgcga cattcaatcg tgttgatgtt cgtcgcccgt cgctggcgct gaccgccaac
    23221 agcagcacgg tgaatatcag cgaactgagt gcatttactg aaaaaggcat tctggaagcc
    23281 accgcaagtg tttcacaaac gccacaacgt cagacccata tcagcctgaa tggacgcggt
    23341 gtgccggtga atattttgca acaatgggga tggcctgaat taccgttgac tggcgacggc
    23401 aatattcagc ttaccgccag tggagatatt caggccaatg ccccgctgaa acctacggtt
    23461 agcgggcaat tgcatgccgt gaacgccgca aagcagcaag tgactcaaac catgaatgcg
    23521 ggcgtcgttt ccagcggtga agttacatcg acggagccgg tgcggtaaac atcaatctcg
    23581 ccgggtacga ctcagacgta cccggcattc cgtcaataga ttacaacgta attgtcagcg
    23641 cattcccttg aggcgtcacc accagcccct gctcactttc agcctgcgaa ccgccttgca
    23701 gaccatttac tttcacaaca ttgcgcaagc acagcgtcca gttcttcgcc tcgcccgcac
    23761 cagtcacagt aatcgtgttg ccagtacgtg ctgcttttaa agtaaagatc accgatccgt
    23821 cagcagcagg cacttcacag acggcttcat gcccgtcttg cagattaaag aggtggaatg
    23881 ccgtgccttc gtgccacacg taatcgggac gttgatcgtt gttgcccagc gccagtagag
    23941 tgttatcacg cacataaacg ggcagactca ggaagccgtg ctgctgttta tgccagcgac
    24001 taccgtcgag ttcatcgttg tgccacaggt gtgtccagcg accttccggc aggtagaact
    24061 gcacatcgcc cgcttcagtg aacaccggcg caaccatcac gttgtcgcct aacatgtatt
    24121 gacggtcaag gtaatcacaa gccggatcgt ccgggaactc catcatcatg gcccgcatca
    24181 tcggcgtacc ccgcgcgttc gcacgcgcag cttcacgata cagatacggc atcatgcggc
    24241 atttcagttg cgtgaagaag cgcaccacat cacaggactc atcatcgtag gcccacggca
    24301 cacgataaga tttgctaccg tgtaaacggc tatggctgga gagcaaacca aacgcgcacc
    24361 agcgtttgta aacgtgcgcc ggagcggtat tttcaaagcc gccgatatcg tggctccaga
    24421 agccaaaacc tgaaaggcca atagacaaac caccgcgcag gctttccgcc attgattcgt
    24481 agttagcgta acaatcgcca ccccagtgta ccgggaattt ctgcgcaccg acggaggccg
    24541 agcgggcaaa caagacagct tcttcctcac caacggtgtc cttgagcacg ttccacacca
    24601 gttcgttgta gatgtacgca taatggttat gcattttctg cgaatcggaa ccgtcaaacc
    24661 actgaacatc aattgggata cgttcgccaa agtcggtctt aaagcaatca acgcccatcg
    24721 cgaccagacc tttcagtttg tcggcgtacc atttgcaggc atccggattg gtaaagtcat
    24781 aaatcgccag acctggctgc catttatccc actgccatag cgaaccgtcc gggcgtttga
    24841 gtaaatagcc tttctcttgt aactctttaa agacggggga tttttgaccg atataggggt
    24901 taatccagac gcagattttc agccctttcg ctttcaggcg gcggatcatc ccttccgggt
    24961 cagggaaagt cagcgggtcc cactcaaaat cgcaccactg gaaggctttc atccagaaac
    25021 agtcaaagtg gaaaacatgc agcggcagat tgcgttccgc cataccatcg ataaagctgt
    25081 ttaccgtcgc ttcgtcgtag ttggtggtaa atgaagtggt tagccacagg ccgaaggacc
    25141 acgggggcgg cagcgccgga cgaccagtaa agcgggtata acgatcaagt accgctttcg
    25201 gcgtcgggcc gtcgataaca aagtattcga gatattcact ctcaacgctg aactgcactt
    25261 tggagacttt ctccgatccc acttcaaaag agacacactg gggatgattg accagtaccc
    25321 cataaccacg gttagtcatg tagaacggga tatttttata cgcctgttca gtacttgtgc
    25381 cgccgtcccg gttccaggtc tctaccgtct ggccattgcg caccagggca gtaaagcgct
    25441 ctcccagacc gtaaactgtt tcgccaacgc caagatcaag ccgctcaaac atataattgc
    25501 gttgattatt cgtgtcctgc acgtagccat tatttttcac ctgactaccg gtaatacgtt
    25561 cgccgttgcg cagaaaatcc agtgaccaga actcaccttt gctgacacgc gcgcttaagt
    25621 tgccactttt aaactcagca taacgttctg tgttttcgat tgtgaccttc acgtcctgca
    25681 aaatattgag cggataatga ggaccgttat tcagcgcccc ctgaaaatgc tcaatccgca
    25741 caccgacaat accttcctgt ggggagaaaa agcgcagcgt aaataaaggc gtatcaagct
    25801 gccaggtacg ttcacgcaca tcacgggggg cagcatagac caccatttca ttatcctgct
    25861 gttcaacctc gaatacctga agcgggtgaa tcaaattgag gccaggttga atcaaccagt
    25921 ttccatcgct aattttcatt gccgttcctt agttctgcac ttcttgagag gtgaattgtt
    25981 gctggcaacg gcgtttaccc tgagccagct gttccataac ggttttcaga ttgtgcgtcg
    26041 tgagtgagta gtagcgttta gcgataatcg cgctcagcaa ataacagatc gccggaacaa
    26101 tcgtgaatag cgcaataatg atgctaatcg tggcgctgtt ctgcgctttt tctgccgcat
    26161 catatccgcc ataagccagc atccagccga taagagcgcc gccgaaggcc aaccccagtt
    26221 tgagcacaaa cagcgtgcca gcaaaactga tcccggtcag gcgtttacca ttgcaccact
    26281 cgccgtagtc gacggtatcg aacatcatta cccactggat aggtgtcacc agttgatgca
    26341 acacaccaat cacgaagatg aagacaaaca tagtgatgct ggcctgcatg ggaacaaaga
    26401 acatcgcgag gctaatcact gccagcaggg cgttcgtcca ccagaagata gtgactttac
    26461 atttccagtc ggtcagaggt tttgccagtg cggaaccaat caggttaccc acgcaataag
    26521 tggtgagaaa agcgacaaac acttccggcg tgcccaaaat ccatgtgacg taatacatca
    26581 tcgccccacc gcgtacgcac accgccagga tattgaaaat ggttagtaaa ccgacaatcc
    26641 gccactggtc gttttgccag atatcacgta aatcttcccg catagacgtt gttgtaggtg
    26701 gtgcttcaac gcgttcttta gtggtgaaga aacaaaatgc cagcatcatg aatgccacca
    26761 cggaaaggac cacgataccg ccctggaaac cgagtggttt attatcaccg ccaattaaat
    26821 taaccagtgg catcatcaga acagtagaaa gcatgcctcc cgcggtcgcc agcacaaaac
    26881 gccaggattg cagcgagata cgctgagtcg ggtcattggt gattacacca cccaatgcgc
    26941 agtaagggat attgacgacg gtataaagta aggtaagtta gggtgtaagt aattgctgca
    27001 tagatcattt tgccgttcat actgagatct ggcgtgctat aggccagtac acagacgatc
    27061 ccgaatggca gtgcgccaaa cagtacccac ggacgaaatt taccccagcg agagcgcgtt
    27121 cgatcggcca acaaccccat gcaaggatcg gaaatcgcat ccagtgcacg agcgaccaaa
    27181 aacatggttc cgacaaatcc ggcaggaatg ccaaaaatat cggtataaaa gaacatcata
    27241 tataacatta cgttatcgaa aataatgtgg ctggcggcgt ctcccatgcc ataaccaatt
    27301 ttctctttaa cggacaacac ttcactcctc atctgctttt ccttgatgct ataggggtgc
    27361 tgagaccgat aacgcatttt gtaaaccatc cctgtgagag cgaatattcc gatatctggt
    27421 tattaattta tgattcttgt tttatgtgat cgtggtagcg ttaattccgc tcatatatca
    27481 ttgtaaaata tgggttttat ataaattata atgctttcgt gataatacgc tgcgtttatt
    27541 aggcggaaaa aactgatctg ggggatgtag aaactcaagg aagtagctat aatgcgcccc
    27601 gcctccatgt agcaatcgag gcgcggaaga tcgtcgtctc cggtgaggcg gctggacttc
    27661 aaatccagtt ggggccgcca gcggtcccgg gcaggttcga ctcctgtgat cttccgccag
    27721 tttacctctt ctgaactctc ccaatgtcaa taaatctctt ctaatccctt gtatatcagt
    27781 atttatttgt atcctgaggt caacctactt caccccgtat caagtttctt ttgggggtac
    27841 tttagggggt gttatttcct attgaaatac aagcaccccc ataataggaa aaatgtgata
    27901 cataacaacg gcttttaaca atatctttgt tatacaaggg gtgtattggg ggtatcaggt
    27961 tagttactgg cagaaatccc cctcaggaac atgaattcat caaaagccgg aggatgtcat
    28021 ggcattaacg atatcaaagt gaaaaccgca aaacccaaag acaaacccta taagcttgct
    28081 gatggcggcg gtatgtatct actgattaac actaatggtt ctaaatactg gcgcatgaag
    28141 taccgctttg ctggtaaaga aaagatgctt tctattggcg tataccccga tgtaacgcta
    28201 gccgatgctc gcgagaaacg cagtgaagcc aggaaactcc tcgctgctgg aggcgatcca
    28261 ggggaggcaa aaaaggaaga aaaaatcgcg cagcaaatga gtctgaaaaa tacgttcgag
    28321 gctattgctc gcgaatggca ccagttaaaa gctgatcggt ggtcattacg ctatcgtgat
    28381 gaaatcattg atacctttga aaaagacatt tttccctaca ttggcaagcg ccctattgcc
    28441 gaaatcaaac caatgaagtt actggaagca ctgcgtaaaa tggaaaaacg tggagctctg
    28501 gagaaaatgc gcaaggtccg ccagcgctgt ggtgaggtgt tccgctatgc aattgttact
    28561 ggccgggcag actataaccc agcccccgat cttgccagtg ctttagctac accgaaaaaa
    28621 gtacattttc cctttcttac tgctaatgaa cttccccact tcctcaacga tctggcgggt
    28681 tataccggaa gtataatcac taagacagct actcagatca ttatgttaac cggcgtacga
    28741 acacaagaac tgcgttttgc acgctgggaa gatatcgatt ttgagacaaa attgtgggag
    28801 atcccggcag aagtaatgaa gatgaagcga cctcatattg tgccgctctc tgagcaggtc
    28861 attatgctat ttaagcagct tgaaccaatc tcaaaacatc atcccctagt ctttatcggt
    28921 agaaatgatc cccgcaaacc aattagtaag gaaagcatta accaagtaat tgaattactt
    28981 ggatataagg gaagactcac agggcacggt ttcagacaca ccatgagtac aattctgcat
    29041 gaacaaggct ttaattccgc ctggattgaa atgcagttag ctcatgtgga taaaaattcc
    29101 atcaggggta cctataatca tgccctgtat ctcgatggcc gccgtgaaat gatgcaatgg
    29161 tacgctgatt acattgattc actctccagc cgggaaagtt aagtcagaaa tacttttccc
    29221 tgatgttttc ctcaattaag tattttatcc tctggcctac aaggctggag gatttttgtt
    29281 attatattat ttttttgcgt cttcaaactt attacaaaaa cataattaaa aacaaattga
    29341 catttttaaa ttggcaatta caaaatacag ccttgcagtt atttcgatac ctttagcaaa
    29401 ggaatagttg attcagatat aatttagcat ttcaatcatc tgacatcatt ataaaaaatg
    29461 agattcacat aaagataaaa ttattgttct caaggaaagc atgaaggggc ttcgtaagca
    29521 atattcattc ggtgccaaga caggacttga tacacagaat ctcaactttt atgctctcag
    29581 actatatcac cacatgtttt acaggctcaa aaaatgaacg atagattatg ctttgaagtg
    29641 cacgataaca agggttattt cgtatttccg gatacctggt ttggcccatt gctgggggag
    29701 tttgaagaag tgcttgatgc atacgacgct gatgaaatca gcgagacgag ttacatcaac
    29761 aaactccgac gccttgctca acgggagccg gactttattg atatacacgc tcatctggca
    29821 tatgcatttc tggaacagaa tgccccgcgt aaggcactga acgccgctct taaaggactg
    29881 gcggcaggta atcgtatcat cccggaaagt ttctgtggtg aaataatctg gatgcatccc
    29941 gaaaaccggc cttatctgcg cgcgctttat gcagcaattc tcgcaaatgt gcacttacag
    30001 cgccatcagg atgcggttat gctcactgat aaaatactgg catacaatcc tgaagataat
    30061 cagggggctc gctggttgtt gggttcagag ctgttacgca ccggcgatca tgagcgagcc
    30121 ttcagtgtac tgaaagagca tgctgatgaa ttttcacctt actggtatga actcggtctc
    30181 ctgcattttc tgaacgggga acatgttaag gccgccacag cttttcgcca tggctttgcc
    30241 acaaacactt acatcgcaga aatgctgtgc ggaaatctgc atccatttcc acttgctgtg
    30301 tggcatgact tctccggctc tctggatacg gcagaagatt actatgcaac atactcacca
    30361 ttatggggac aatatccgga agcattgctg tttgtaaact ggctctataa ccattcctcc
    30421 gtgctgcatg aacgatcaga aatcattaaa tgcgccgaaa tgctgattca ggaagatgat
    30481 ttcgaaatat gcgaaagtat tttaagacag caggagcacc tgtggaaaag aattgacaag
    30541 acactttctg aggaaattgt acagaaatgc agaaatatga acggtgaata tatctggccc
    30601 tggatattgc ccttttccgc agcagggatg aaacattcaa gcatccagta tcagtagata
    30661 ttgcattagt gtaactttaa aacaagtaat aatttattat ccaataataa cactgtgttc
    30721 cttccccagg tgattaccct ggggttattt tctgaaagtt tcctttgcaa aaggatagcg
    30781 ttgtaaatta ctttaacgaa ttactccatg aactcattaa agttctttcg taatacttta
    30841 tcgtcaaaca cgttatagct ggcatcaaaa accacagatt taccctgcca gcgggtaatc
    30901 ccctcctctg gcgttttcgg ttgaactggt atccccgtcc aacacaggca gccattataa
    30961 acagtaaaaa attgtattcg ctgttatgaa atcaatagtc ctgacgtact gttgaatgtg
    31021 cttgtgttct gaacctcgta caaccagtac atttgatatc gaattataaa caaaacattg
    31081 catccaactt ttttaatatg gacaatagtt gtccatacgg tggttttata ttcgataaat
    31141 caatggattt cattgagtca caggactggt aatggatgaa aatgctttaa ggtttgcctc
    31201 atactggcgc aactcgcttg cagatgctga gtcaggaaag ggctgttttg aacggaaaga
    31261 cgcccaaaat ttcactcact ggcatgggat agcggcggga cgtcttgacg aagcgattgt
    31321 caataaattt tttaagggag aaaaagacga tgtcgaaact gtcaatgtca tcttacgccc
    31381 aaaagtttat ttccggttac tgcagcatgg taaggaccgt tccgcaggca cgcctgatat
    31441 tgttac
//
LOCUS       DAJTRD010000039        31189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTRD010000039 DAJTRD010000000
VERSION     DAJTRD010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.039"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            393..2051
                     /locus_tag="OO315_002013"
     CDS             393..2051
                     /locus_tag="OO315_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5890936.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2048..3004)
                     /locus_tag="OO315_002014"
     CDS             complement(2048..3004)
                     /locus_tag="OO315_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5890937.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3155..4270
                     /gene="macA"
                     /locus_tag="OO315_002015"
     CDS             3155..4270
                     /gene="macA"
                     /locus_tag="OO315_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5890938.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4267..6213
                     /gene="macB"
                     /locus_tag="OO315_002016"
     CDS             4267..6213
                     /gene="macB"
                     /locus_tag="OO315_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5890939.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6286..6510)
                     /gene="cspD"
                     /locus_tag="OO315_002017"
     CDS             complement(6286..6510)
                     /gene="cspD"
                     /locus_tag="OO315_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5890940.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6833..7153
                     /gene="clpS"
                     /locus_tag="OO315_002018"
     CDS             6833..7153
                     /gene="clpS"
                     /locus_tag="OO315_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5890941.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7184..9460
                     /gene="clpA"
                     /locus_tag="OO315_002019"
     CDS             7184..9460
                     /gene="clpA"
                     /locus_tag="OO315_002019"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5890942.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9863..9950)
                     /locus_tag="OO315_002020"
     tRNA            complement(9863..9950)
                     /locus_tag="OO315_002020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9914..9916),aa:Ser,seq:gga)
     gene            complement(10204..10422)
                     /gene="infA"
                     /locus_tag="OO315_002021"
     CDS             complement(10204..10422)
                     /gene="infA"
                     /locus_tag="OO315_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5890943.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10707..11411)
                     /gene="aat"
                     /locus_tag="OO315_002022"
     CDS             complement(10707..11411)
                     /gene="aat"
                     /locus_tag="OO315_002022"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5890944.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPDDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11453..13174)
                     /gene="cydC"
                     /locus_tag="OO315_002023"
     CDS             complement(11453..13174)
                     /gene="cydC"
                     /locus_tag="OO315_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5890945.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13175..14941)
                     /gene="cydD"
                     /locus_tag="OO315_002024"
     CDS             complement(13175..14941)
                     /gene="cydD"
                     /locus_tag="OO315_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5890946.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15064..16029)
                     /gene="trxB"
                     /locus_tag="OO315_002025"
     CDS             complement(15064..16029)
                     /gene="trxB"
                     /locus_tag="OO315_002025"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5890947.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16573..17067
                     /gene="lrp"
                     /locus_tag="OO315_002026"
     CDS             16573..17067
                     /gene="lrp"
                     /locus_tag="OO315_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5890948.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17202..21230
                     /gene="ftsK"
                     /locus_tag="OO315_002027"
     CDS             17202..21230
                     /gene="ftsK"
                     /locus_tag="OO315_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5890949.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21385..21996
                     /gene="lolA"
                     /locus_tag="OO315_002028"
     CDS             21385..21996
                     /gene="lolA"
                     /locus_tag="OO315_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5890950.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            22007..23350
                     /gene="rarA"
                     /locus_tag="OO315_002029"
                     /gene_synonym="mgsA"
     CDS             22007..23350
                     /gene="rarA"
                     /locus_tag="OO315_002029"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5890951.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23441..24733
                     /gene="serS"
                     /locus_tag="OO315_002030"
     CDS             23441..24733
                     /gene="serS"
                     /locus_tag="OO315_002030"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5890952.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24973..27416
                     /gene="dmsA"
                     /locus_tag="OO315_002031"
                     /pseudo
     CDS             24973..27416
                     /gene="dmsA"
                     /locus_tag="OO315_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27427..28044
                     /gene="dmsB"
                     /locus_tag="OO315_002032"
     CDS             27427..28044
                     /gene="dmsB"
                     /locus_tag="OO315_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5890953.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28046..28837
                     /locus_tag="OO315_002033"
     CDS             28046..28837
                     /locus_tag="OO315_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5890954.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28873..29499)
                     /locus_tag="OO315_002034"
     CDS             complement(28873..29499)
                     /locus_tag="OO315_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5890955.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29814..30962
                     /locus_tag="OO315_002035"
     CDS             29814..30962
                     /locus_tag="OO315_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5890956.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFSAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT         7692 a         7644 c         8373 g         7480 t
ORIGIN      
        1 aatatggtcg ttttataggg tcgtcgtaaa aaatgacgac ggaaataatg cgcggctatt
       61 ttaaaaacga aggcgagtca ttcaccagat aaataaatcc agtaaatttg atttagggca
      121 acagcgggtt gccccatata gtcatttgtc tgattgacag tgtagtgcaa gcaaaagatt
      181 taatccttta ggcgtaataa aaaataattt atcatgctaa ttatttgatt ttgttgtttt
      241 tgcgggctta tcagcaagag ggagtataac gcgattattc gctcattttt cagacaattg
      301 ccatgcttaa atgtgatgtc atcacgtatt agcaaggcct ttcccgttat actgcgagcg
      361 taaaggataa gtcacatatt tctggagggg atatgattct tgagcgcgtt gaaattgtgg
      421 gttttcgcgg tatcaaccgt ttgtcgttga tgctcgaaca aaacaacgtc ctgattgggg
      481 agaacgcgtg gggtaaatcc agcttgctgg acgccttaac cctgctgcta tcgccagaat
      541 cagatctcta ccattttgag cgcgacgatt tctggttccc gccgggagat atcaacgggc
      601 gagaacatca tctgcatatt attttgacct tccgcgaatc gctgccaggc cgacatcggg
      661 ttcgccgtta tcggccgctg gaagcgtgct ggacgccatg caccgatggc tatcaccgta
      721 ttttttatcg tctggaaggg gagagtgcgg aagacggcag cgtgatgaca ctgcgcagtt
      781 ttctcgataa agacggacat ccgattgatg ttgaggatat taacgatcag gcacgccatc
      841 tggtgcgttt aatgccggtg ctgcgcttgc gtgatgcccg ttttatgcgc cgtattcgta
      901 acggcacggt gccaaatgtc cccaatgtgg aagtcaccgc gcgccagctc gatttcctcg
      961 cccgtgagtt gtcctcacat ccgcaaaatc tctctgatgg acagattcgt cagggacttt
     1021 ccgcaatggt gcagttgctt gagcattatt tctctgagca gggggctgga caggcgcgat
     1081 atcgtttaat gcggcggcgg gccagcaatg agcaacgaag ctggcgctat ctggacatca
     1141 tcaaccggat gattgaccga cctggagggc gctcgtatcg ggttattttg ctcggcctgt
     1201 ttgctacttt attgcaggca aaaggcacat tgcgactgga taaagacgcg cgtccgttat
     1261 tgctaattga agatccagaa acccgcctgc atccgattat gctttccgtt gcctggcatc
     1321 tgttgaatct tttgccttta cagcgtattg ctaccaccaa ctctggcgag ctgctttcgt
     1381 taactccagt ggaacatgtt tgccgcctgg tgcgtgaatc ttctcgtgtt gccgcctggc
     1441 gtctggggcc aagtggcttg agcaccgaag acagccgccg catctctttt cacattcgtt
     1501 ttaatcgtcc gtcatcgctg tttgctcgct gctggttgct ggtggaaggg gaaacggaaa
     1561 cctgggttat caatgaactg gcgcgtcagt gcggacatca ttttgatgcc gaagggatca
     1621 aggtcattga gtttgcccag tccgggctaa agccactggt taaatttgcc cgccgaatgg
     1681 ggattgaatg gcatgtactg gtcgatggcg atgaagcagg gaagaaatat gccgctacgg
     1741 tacgcagcct gttgaataac gatcgggaag ccgaacgaga acatttaacg gcgttaccgg
     1801 cgctggatat ggaacatttt atgtatcgcc agggattttc cgatgtgttc caccgcgtgg
     1861 cgcaaatccc ggaaaatgta ccgatgaatc tacgcaaaat tatctcgaaa gcgatccatc
     1921 gctcttccaa acccgatctt gccattgaag tggcaatgga ggcaggacgt cgtggtgtgg
     1981 actccgtacc aacgctgctg aaaaaaatgt tctcacgcgt gctgtggctg gcgcgcggtc
     2041 gcgcggatta accgcgaaac atcgtggcca tttgtggctg aatagcgtcg agcatctcat
     2101 agcgccgacg gtattcagcc cgttttttac tggcgatttc ggcaatctct tttcgtgcta
     2161 tctgtgctgg aaggcggtaa tggcgttcag catcacatac gccgccaacc gactcccaga
     2221 acgcgttgta atcagcatgg atcttgcctt ctttatcgcg ataacgcagg ctgcggtaaa
     2281 tatgcgtttc attgctgacg gcaataatct gctctacctg caaacgttgg gcaaacagac
     2341 aggccgcttc catcacgagg cgtttgggaa atagcccgtg gcaggctttc gtcgcattct
     2401 ggatttcctg atgtggaatt tcccattttg cgccttgcag tccgccaata aacatcgttc
     2461 ttttcccctg atattcacac agggtaaacg tgatctctgc cagaggaata ccttcgctgt
     2521 tgcggaacag gattgtgctg tcaccttctt tatccattga gatcatcatg gtcagctcaa
     2581 gcgtgaactg ctcaccgttt ttgccttcca gtttagccag ttgcagcccg ggggtattca
     2641 aatataagct gaattcttcc gccgacatac acccgcggag taacgcataa tggtaacgta
     2701 acgcctccag caattgctta cggctaagat tcgcggcaag gtaagggcga tgcagacgca
     2761 caggcagtcg cggctggcgc gttaacaaca cattgagatt aggccagtgg gaaagttcgt
     2821 tcatccactc aacgcttaaa cgcggcataa tcaacgagcg cagcaaaaat ttctggcgaa
     2881 aactacagcg atgccagaat ttacccggcc gacactgtcc acgtgccaga ctaagaaaaa
     2941 gtgacaggct gctgagagat tcagatggcg taaaggtccg ttcagttagc tgcgacatat
     3001 tcatgaaatc aatggttata catgacgtcg atttcaccat tgcgtatctt aaccaaacat
     3061 caatagtgtg attactaacg taaattttag ggttttgttg atatttcgtt gaagttaatg
     3121 acccggattg gcatatggag tattcagaat atttatgaaa aagcggaaaa ccgtgaagaa
     3181 gcgttacgtt attgcgctgg tgatagtcat cgccggactg attacgttat ggagaattct
     3241 taacgcaccc gtgccgactt atcagacact gattgtgcgc cccggtgatt tacagcaaag
     3301 cgtgctggcg accggaaagc tggacgcgct gcgtaaggtt gacgtgggcg cgcaggtcag
     3361 cggtcagttg aaaacgctgt cggtggcgat tggcgataaa gtaaaaaaag accagctttt
     3421 aggggtcatt gatcctgaac aggctgaaaa ccagatcaag gaggtcgaag caacgctgat
     3481 ggagctacgc gcgcagcggc agcaggcgga agcggagctg aaactggcgc gggtgacgta
     3541 ttcccgtcag caacgtctgg cacaaacgca agcggtttca ctgcaggatc tcgataccgc
     3601 cgcgacggag atggctgtga aacaggcgca aattggcacc attgacgcgc aaatcaagcg
     3661 caatcaggcc tctctcgata cggctaaaac caatctcgat tacacccgta tcgttgcccc
     3721 gatggctggg gaagtgacgc aaatcaccac tctgcaaggc cagacggtga ttgccgcaca
     3781 gcaagcaccg aacattctga cgctggcaga tatgagcact atgctggtaa aagcgcaggt
     3841 ttctgaagcg gatgtaatcc acctgaagcc ggggcaaaaa gcctggttta cggtgcttgg
     3901 cgatccactg acgcgctacg aggggcaaat caaggatgtg ctaccgacgc cggaaaaggt
     3961 aaacgacgct attttctatt acgcccgttt tgaagtaccc aaccccaatg gtttgctgcg
     4021 gctggatatg accgcgcaag tgcatattca gctcaccgat gtgaaaaatg tgttgacgat
     4081 ccctctgtcg gcgttaggcg atccggttgg cgataatcgt tataaagtca aattgttgcg
     4141 taatggtgaa acacgcgagc gtgaagtgac gattggcgca cgtaacgata ccgatgttga
     4201 gattgtcaaa gggctggaag cgggcgatga agtggtgatt ggtgaggcca aaccaggagc
     4261 tgcacaatga cgcctttgct cgaattaaag gatattcgtc gcagctatcc tgccggtgat
     4321 gagcaggttg aggtgctgaa gggcatcagc ctcaatattt atgcgggtga gatggtcgcg
     4381 attgttggcg cttcgggttc cggtaaatcg accctgatga atattctcgg ctgcctggat
     4441 aaggccacca gcggcaccta tcgcgtagcc ggtcaggata ttgccacgct ggacgccgat
     4501 gcgctggcgc aactgcgccg cgagcatttc ggctttattt tccagcgtta ccatttgctt
     4561 ccgcatttaa ccgtcgagca gaacgttgaa gtacccgccg tctatgccgg tcttgagcgg
     4621 aaacagcgac tgcttcgtgc ccaggagttg ctgcaacggc tggggctgga agaccgtaca
     4681 gagtattatc cggcacagct ttcgggtggt cagcaacagc gcgtcagcat cgcgcgggca
     4741 ttgatgaacg gcggtcaggt aattcttgcc gatgaaccga ccggcgcact ggacagccat
     4801 tctggcgaag aggtgatggc gatcctgcat cagctgcgcg atcgcgggca tacggtgatt
     4861 atcgtcaccc acgatccgca ggtcgctgct caggccgagc gggtgatcga aattcgcgac
     4921 ggcgaaattg tgcgcaatcc tcccgccatt gaaaaagtga atgttgctgg cgggacggag
     4981 cctgttgtca atacagtgtc tggctggcgg cagtttgtca gcggttttaa cgaggcgctg
     5041 acgatggcat ggcgggcgct ggcagcgaat aaaatgcgta ctttactgac catgctgggg
     5101 attattatcg gtattgcgtc ggtggtttcc attgtcgtgg tgggtgacgc cgccaaacaa
     5161 atggtgctgg cggatattcg ttctattggt acgaatacta ttgatgtcta tcccgggaaa
     5221 gattttggcg atgacgatcc gcaatatcag caggcgctga agtacgacga cttaatcgcc
     5281 atccaaaaac aaccgtgggt cgcctcagcc acacctgccg tctcgcaaaa cctgcgcctg
     5341 cgttataaca atgttgatgt tgctgccagt gccaatggcg tgagcggcga ttattttaat
     5401 gtctatggca tgaccttcag tgaaggaaac acctttaatc aggagcagct gaacggtcgt
     5461 gcgcaggtcg tggttctcga cagtaatact cgccgccagc ttttccccca taaagcagat
     5521 gtggttggcg aggtgattct ggtcggcaat atgcccgcca gagtcattgg tgtggcggaa
     5581 gaaaaacagt cgatgtttgg tagcagtaaa gtgctgcgtg tctggctacc ttacagcacg
     5641 atgtccgggc gagttatggg ccagtcgtgg cttaactcca ttactgtcag ggtgaaagaa
     5701 ggatttgaca gcgccgaggc ggaacagcaa ctcacgcgtt tactttcact gcgccacggt
     5761 aagaaggatt tctttacctg gaacatggac ggcgtcttga aaactgttga aaagaccaca
     5821 cgtactttac aactgtttct gacgctggtg gcggtgattt cgctggtggt gggcggtatt
     5881 ggtgtaatga atattatgct ggtgtcagtg accgagcgga cgcgggaaat tggcattcgc
     5941 atggctgtag gtgcgcgagc aagcgatgtt ttgcaacagt tcctgatcga agccgtactg
     6001 gtttgcctgg tcggtggcgc gttgggaata acactgtcac tgttaattgc tttcaccttg
     6061 cagcttttct tacccggctg ggagattggt ttttcaccgt tggcgctgct gctggcgttt
     6121 ctctgctcga cggccaccgg gattttattt ggctggttac ccgcacgaaa tgcggcacga
     6181 ctggatccag tagatgctct ggcacgggag taatttttga gataaaaatg ccagccgatc
     6241 gggctggcat tttgccttta ggatgtacac aatgagacag aagagctatg cgactgccgc
     6301 ttctacttcg acgggcacaa taacactggc gtgattgcct tttggcccct ggtggacatc
     6361 aaactgaacg gattgtccag cttttagcgt tctgtaacca tccatctgaa tggtggaata
     6421 atgagcgaaa atatcttcgc cgccgccttc agggcagatg aaaccaaacc ctttggcatt
     6481 gttgaaccac ttaacagtac ccttttccat gcttcgacat ccttcgcaaa tcttatacaa
     6541 gtaagatgga ataaaccggg gtcagagagg gggctgttca aaacctcgcc aactctagaa
     6601 atacaattta gagaattagg gcgagccgtc aagcatttga caggggccag ggggcaggta
     6661 tgaatcaaaa atttgaagca gttaacgcta ttgacaggaa tgtgacagat gtcgctgatg
     6721 ccaacgatag atgatagtta tctatcatgt ggagtagatt ggtcaggcaa ataagctctt
     6781 gtcagcggca gggcgttctg ccgataaccg taaccgaaga tgataactga caatgggtaa
     6841 aacgaacgac tggctggact ttgatcaact ggcggaagaa aaagttcgcg acgcgctaaa
     6901 accgccatct atgtataaag tgatattagt caatgatgat tacactccga tggagtttgt
     6961 tattgacgtg ttacaaaaat tcttttctta tgatgtagaa cgtgcaacgc aattgatgct
     7021 cgctgttcac taccagggga aggccatttg cggagtcttt accgccgagg ttgcagaaac
     7081 caaagtggcg atggtgaaca agtacgcgag ggagaatgag catccattgc tgtgtacgct
     7141 agaaaaagcc tgaatgcagg cataaaaatt gggggaggtg cctatgctca atcaagaact
     7201 ggaactcagt ttaaatatgg ctttcgccag agcgcgcgag caccgtcatg agtttatgac
     7261 cgtcgagcac ttgttactgg cgctgctcag taacccatct gcccgggagg cgctggaagc
     7321 gtgttctgtg gatttggttg cgctccgtca ggaactggaa gcctttattg aacaaaccac
     7381 acccgttctg cctgccagtg aagaggagcg cgacacacag ccgacgctga gttttcagcg
     7441 tgtactgcaa cgtgcggtct tccatgtcca gtcctccggt cgcaatgagg ttaccggtgc
     7501 aaacgttctg gtcgctatct tcagcgaaca ggagtcgcag gcggcatatc tgttgcgtaa
     7561 acatgaagtc agccgtctcg atgtggtgaa ctttatctct catggcacgc gtaaagacga
     7621 gccgacacag tcttctgatc ctggcagcca gccaaacagc gaagaacaag ctggtgggga
     7681 ggaacgtatg gagaatttca cgacgaacct gaaccagctt gcgcgtgtgg gcggaatcga
     7741 cccactaatt ggtcgtgaga aagagctgga gcgtgctatt caggttctct gccgtcgccg
     7801 taaaaacaac ccgctgctgg tgggggaatc tggtgtcggt aaaaccgcga ttgcagaagg
     7861 tcttgcctgg cgaattgttc agggtgatgt gccggaagtg atggctgact gtacgattta
     7921 ctctctcgat atcggttctc tgttagcggg cacaaaatat cgcggcgact ttgaaaaacg
     7981 ttttaaagcg ttgctcaagc agctggagca ggacactaac agcatcctgt ttattgatga
     8041 gatccacacc attatcggtg cgggtgcagc gtctggtggt caggtcgatg cggctaacct
     8101 aatcaaaccg ttgctctcca gcggtaaaat tcgcgtaatt ggttcgacaa cctatcagga
     8161 gttcagcaac attttcgaga aagaccgtgc tctggcgcgt cgcttccaga aaattgatat
     8221 tactgaaccg tcgatcgaag aaactgttca aatcatcaat ggcctgaaac cgaagtatga
     8281 agcgcaccac gacgtgcgtt ataccgcaaa agcggtgcgt gcggcggtag agctggcggt
     8341 gaaatacatt aacgatcgtc atctgccgga taaagccatt gatgttatcg acgaagcagg
     8401 cgctcgcgca cgtctgatgc cggtaagcaa acgcaagaaa accgttaatg tggcggatat
     8461 tgagtccgtg gtggcccgta ttgcacgcat tccagagaag agtgtttctc agagtgaccg
     8521 cgataccctg aaaaaactca gcgatcgcct gaaaatgctg gtcttcggcc aggataaagc
     8581 cattgaggcg ctgactgaag ccattaagat ggcgcgtgca ggtttaggtc acgaacataa
     8641 accggtcggt tcgttcctgt ttgccggtcc taccggggtc gggaaaacag aggtgacggt
     8701 acagctttcg aaagcgttgg gcattgagct tctgcgcttt gatatgtccg agtatatgga
     8761 acgccatacc gtcagccgtc tgattggtgc gcctccggga tacgttggtt ttgatcaggg
     8821 cggtttgctg accgatgcgg taatcaagca tccacatgcg gtgctgctgc tggacgaaat
     8881 cgagaaagcg catccggacg tgttcaatat tctgttgcag gtgatggaca acggtacgct
     8941 gaccgataac aacggacgca aagcggactt ccgtaacgtg gtgctggtga tgaccactaa
     9001 cgccggggta cgggaaactg agcgcaaatc cattggtctt atccaccagg ataacagcac
     9061 cgatgcgatg gaagagatca agaagatctt tacaccggaa ttccgtaacc gtctcgacaa
     9121 cattatctgg ttcgatcatc tgtcaactga cgtgatccat caggtggtgg ataaattcat
     9181 cgtcgagttg caggttcagc tggatcagaa aggtgtttct ctggaagtga gccaggaagc
     9241 gcgtaactgg ctggccgaga aaggttacga ccgggcaatg ggcgcacgtc cgatggcgcg
     9301 tgtcatccag gacaacctga aaaaaccgct cgccaacgaa ctgctgtttg gttcgctggt
     9361 ggacggcggt caggtgacgg ttgcgctgga taaagagaaa aatgagctga cttacggatt
     9421 ccagagtgca caaaagcaca aggcggaagc agcgcattaa tctgattgtc aggtaggttg
     9481 gtgaagtccg taatctcgtc aggggttacg gactttttat ttatggggtg gaacggtgaa
     9541 aaccctattt ttggaggtga aggtaagttg ttgataatta gtgctgctgg aaggtaagga
     9601 taaaaaaggg tgctgcagga gaatgggatg gttttgcttt attaacaacg ggctaaacgt
     9661 gtagtatttg agttcactgc cgtacaggca gcttagaaat cgacggggtg cggtaaaacc
     9721 tttgcgaacg cgttcactgc cgtacaggca gcttagaaat tcacaggtaa catactccac
     9781 ccgcccacca tgttcactgc cgtacagaca gataaaatgc gaaaaaaaag ctcgcacttt
     9841 cgtacgagct cttctttaaa tatggcggtg agggggggat tcgaaccccc gatacgttgc
     9901 cgtatacaca ctttccaggc gtgctccttc agccactcgg acacctcacc aaattgtttt
     9961 gctaccaaac ctcatgggtg gcaacggggc gctactatag ggagttggag taaaacggtc
    10021 aagaagaatt ttaatgataa ttattgtttg ctcatactgt aaacaacttg tgcagtatat
    10081 ctacatcgag acaagttatg gacttatact tccaaagtac ttcatacata tcacaaaata
    10141 aaaaggccgg ttaaaccgac cttttactcg ttctttctct tcgcccatca ggcggtaaaa
    10201 caatcagcga ctacggaaga caatgcggcc tttgctcagg tcgtacgggg tcagttcaac
    10261 agtcactttg tcgcccgtca ggatgcggat gtagtttttg cgcattttac cggagatgtg
    10321 tgcagtaacc acgtgaccgt tttctaactc tacgcggaac atggtattag gcaacgtttc
    10381 aagaacggta ccttgcattt caatattgtc ttctttggcc atctaatcct ctggggtatc
    10441 actaccgtaa tttgaaccgg caagataatg ccgaagttct gtaaataagt aaagatttgc
    10501 gcgctaaatc gcaacaaaca ggttcggcac attactccga aaacacacgg ctaagccgca
    10561 ccaaaagcgc aacgtataag ggagcggtga gataaacgat gggcgttacc tgacgcgaaa
    10621 aattccttat cggcagcggg gtaatgagcg taaccaactc tgcgaccgca attataacac
    10681 tctggggaga aatgtgccga aaacattcat tcttgtggtg aaaacaagca ccgtggtacc
    10741 cagaaattat tcggcaatcg tccgaggcgc atttgattga gataattaag gtaatcccgg
    10801 cggggaattt cgcaggcacc aagcgatgct gtgtgatcgt taaggacctg gcagtcgata
    10861 agcttaccgc catgaccgat aaattcctca cagaatacca gaagcgccgt tttagacgca
    10921 ttttccatcc ggctgaacat ggactcgcca caaaatagcg ttccctgggc cacgccgtac
    10981 ataccgccga caagctcatc ttcacgccag acttcaatgg agtgggcatg accgagttcg
    11041 tgaaggcgat ggtaggcttc gaccacgcca cgcgtgatcc aggttccttc ttcgcgatcg
    11101 ctggcacagc cttcaatgac ctgaccaaaa gcgtaattca tcgtgacacg atagggcgag
    11161 cgtttatgaa atcgcttcat actacggctg atatgcagtg attctggcca tagcaccgcg
    11221 cggggatcgg gcgaccacca gaggatgggg tcgtctggag aaaaccacgg aaaaatacca
    11281 cgctggtaag ccattaacag gcgcgcaggg ctaagatcgc ccccaagtgc cagcaggccg
    11341 ttaggctcac gtaatgcgcc ttccggggaa gggaaggcta ttgaatggcg agaaagctga
    11401 accaagcgca tgaccgcaaa actccacgca agtcggatcg ttcaataata gcttacaaac
    11461 cctgcttgaa ctggtaataa cgcccctgtc tggcaagcag ttctgcgtga gtaccttgct
    11521 caataatttg cccgttgtcc atcactatta tttgttggaa acgagagagt ccgcgaagtc
    11581 gatgggtgac cattaacacc gttttctcac gcatcatttt tgcaagcaat tcaaggatct
    11641 ggctttcggt tgtggcatct aagccttcgg taggttcatc cagcaacacc agtggcgcat
    11701 catgtaacag cgtacgggcg atagccagac ggcgcagttc accaccggag agctggcgtc
    11761 cgccttcacc taaccaactg ttgagacctg catcctcgag cagcttctcc agaccaacgc
    11821 gacgcaagat ctccgacaga gcctcatcac tactgccagg cgaggcgagt aacagattat
    11881 cacgcagtgt ggcgctaaac agatgcactc gctgaggaac aacgctgatg gtctgtcgta
    11941 gagccgcttc attcaggctg gctatggggc tatcgttaag caaaatctcg ccctgttgcg
    12001 ggtcccatgc gcgggtcagc agttgtaaca gtgttgattt gccgcatccg gttcgcccga
    12061 gaatcgctat atgttccccg gcgtttacct gaagagaaat ccctttaagt gcctgttggg
    12121 attgctccgg ataagtgaac tgaacatccc gtaatgtcag cgaaacgcga tcggcaacac
    12181 gagtttgggt atcagggaag gtgacctccg gtttttgatc cgttaagtca gagatacgta
    12241 cggcagaggc aatgacttgc cccagatgct gaaatgcacc cgttactggt gccagtgctt
    12301 caaacgcggc taacgcacaa aagacaaaca gggcaattaa tgcgccgggt tgagcattgc
    12361 cgccaacgcc gccagacgcc atccacagca tcaggatcac cgctaacgcg ccaatgagca
    12421 gcattatcgc ttgcgacaat gcggtcagtt cagattgacg gcgttgcgct tccagccatt
    12481 gaatttctgt attctctagt tgcgtgcgat aacgatcgct ggcaccaaaa atggtcagct
    12541 cagcttgccc ttgcagccag gccgtcagtt gttggcgata ctgtccgcga agatgagtca
    12601 gattttgccc ggtgcttttt cccgcacgat aaaacagcgg tggcatcagg aaaagcgtca
    12661 gtaacataac gctgcccagc gtaaaggcga gggtgaaatc aaggaaactt aacccgattg
    12721 tcaccaccat aatcaccaca aaagcgccca ccagcggcga gataacgcgc aggtaaagat
    12781 gatcgagcgt atcaacatcc gccaccacgc ggttgagcaa ttcgccctga cgatagcgcg
    12841 ccagtccggc aggggagagg ggcaacaatt tgctgaaggt gtaaatgcgc agatgctgca
    12901 acacgcggaa agtcgcgtcg tgacttacca gacgttcaaa ataacgcccg gcagtacggg
    12961 tgattgcggc accacgcacg cccgcagcgg gtagcatata gttgaagctg tacagtccgg
    13021 caacccccgc aaccgctgag gccgagagga accagccgga aagtgtcaac agaccgatac
    13081 tggcgagcag cgtcacaatt gccagcacaa taccaagact taacatccat ttatgacgtt
    13141 tatacagtgc cagatagggt agcaaagcgc gcatttaaat ctcctcctga cgatgggcca
    13201 gtaatgtggc gaatgggcca ccagccacac ttaattccgc gtaacgtcct tgctcaataa
    13261 tccggccatc ctgcataacc caaatgacat cccagtcagc aagatcttct aactggtggg
    13321 tgaccattaa cgttgtctgg cgcagagagg cggcattcag cgcctccatt acgcgctgtt
    13381 cactgtgagc atcaaggctg gcagcgggtt catccaacag taatagcgaa cagggattta
    13441 gtaacgcacg ggccaccgcc acgcgctgcg cctgccccac ggaaaggcgg gcagcctggt
    13501 cgccaacagg tgtatcaacg ccttgtggca ggagcggtag aaactcgctg acccaggcgt
    13561 tatccagcgc tgcttgtaat tcttgttcgc tggcatcagg tcgcgccagt agtacgttat
    13621 cccgcaattt tgctgccggt aattgtgggt tttgcccaac ccaggagaga tgtttacgcc
    13681 atgattctgg tgataaatca cgtaattcta tcccgttgat tcgtaatgat ccctgatatg
    13741 agagaaaacc agaaagcgcg ttcagcagtg agcttttacc tgaaccgctg cggccaacca
    13801 acactgcacg ttggcctgct ggcaaagtaa agttcagcgg tccggccagc gttttacctt
    13861 ccggcgacgt gataaacaga tcctcggcct caatggtcag cggatcggtc aatgctaatt
    13921 ccgcctcacc gcgctgcgga tgggcgagcg gggtttccat aaacgttttc agactgtcag
    13981 ctgcaccaac agcctgggct ttagcatgat aaaacgtacc gagatcgcgt aatggctgga
    14041 aaaactctgg cgcaaggatc agggccagaa aacctgcagc cagcgtcacc ccagtatcgt
    14101 agtgaccaaa atccagctcg ccgagatagg aaaaaccaaa gtagaccgcc accagagcaa
    14161 ttgacagcga ggtaaaaaat tcgagaatgc cggaggataa aaacgccagt cgtagcactt
    14221 ccattgtccg ttggcggaaa tcttccgaag cagaacgaat actttcaatt tcagcttcac
    14281 cacgaccaaa aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa tgcccactta
    14341 agcgagcaag agcgagaaag ttacgccggt tagcatcggc agcccccatt ccaaccagcg
    14401 ccataaacaa cggaattagc ggtgctgtgc ccagcagaat gagcgccgca gcccagttag
    14461 aggggaagat cgccaccaca atcagcaacg gcaccgacac tgccagcgcc atttgcggca
    14521 gatagcgtgc atagtaatca tgcatatcgt caatttgctc gagtaccagc gtcgcccagc
    14581 tccccgcagg tttaccctga atccacgctg gccctgcttg ttgcagacgg tcgagaacct
    14641 gacggcggat ggcaaagcgg atatgctgcc cggcgtgata acccacccgt tcgcgtaacc
    14701 agaccaccca tgcgcgcaat acaaaggtca gaaccagtaa cgtaaaggga agcagcaggg
    14761 cttcacgggg aatattctcc ataatcatat gttgcagaat tcgcgccatg aaccaggcct
    14821 gggcaatgat caatatgccg cttacaaagc ccagcagacg agaaatattc agccaacgtt
    14881 gggagatgac gctttgctgt tttaaccagc gggttaactc tttttgacga gatttattca
    14941 ttgcacgctt agcaggtgag ttatcagaat tatttgcaga gcaatgttac aacggggaaa
    15001 aaataaaggc gacccatagt cgcaaggtgt cgccttcttt acttttgtta ctgatttgta
    15061 aaattatttt gcgtcagcta aaccatcgag gtagcgttcc gcatcaagtg ctgccatgca
    15121 gccagtaccg gccgaagtaa tggcctggcg ataaatgtga tccatcacgt cgcctgcggc
    15181 aaagacgcca ggaatgctgg tctgggtggc attaccatga atacccgact gtactttgat
    15241 gtagccgttt tccagttcca gctgcccttc gaaaatcgca gtattcgggc tgtgaccgat
    15301 agcaacaaac agaccggcaa cgtcgagtga ctcgatgtta tcgctgtttt gcgtatcgcg
    15361 cagacgaacg ccagtgacac ccatttgatc gccggtcact tcttccagcg tacggttggt
    15421 gtgcagaatg atgttgccgt tctccacttt atccatcaga cgcttaataa ggattttttc
    15481 cgcgcggaaa ccgtcacggc ggtgaatcag atgcacttcc gaagcgatgt tagacagata
    15541 cagcgcctct tcaaccgcgg tattgccgcc gccgatgacc gcaactttct ggttgcgata
    15601 gaagaaaccg tcgcaggttg cacaagcaga aaccccacgg cctttaaagg cttcttcaga
    15661 gggcaggccg agatagcgtg cagaagctcc ggtggcaata atcagcgcgt cgcaagtgta
    15721 ttcgccgtta tcgccattca gacggaacgg acggttttgc agatccacct tgttgatatg
    15781 atcaaaaatg atctcagttt caaacttggt ggcatgttcg tgcatgcgcg ccattaataa
    15841 cggaccggtc agatcgtttg gatcaccagg ccagttttcc acttccgtgg tggtggtcag
    15901 ttggccgcct ttttccatgc cggtaatcag cacaggttgc aggttggcgc gcgccgcgta
    15961 gacagcagcg gtgtatcccg ccgggcctga acccaggata agcagtttac tgtgtttggt
    16021 cgtgcccatg agatccccat agttgttggc agacaatggg caggattgta gggaatttac
    16081 agacgtaaaa aaagagtatg acgattttgt taacaatttg tgcaatcggc agcatcgata
    16141 agcaggtcaa attctcccgt cattatcacc tctgctactt aaatttcccg ctttataagc
    16201 cgattaaatg atgaataaac gcccctgtta atgaatatct ggcatgttgt actaaaaatc
    16261 gatgttttgc tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa gaaaaaaaca
    16321 gtattcttat atgcgcataa ccatgtatgt aaataccatg tttaccgtgc tagtgaaatc
    16381 tacgtatggc gtggacagac gccattcgtg atgtcgatag ctgccacaag gcaacggtct
    16441 tctcaccgta gacccaggca ttgcgcgccg tgaatcttca tgatttcggt ctatcgtgac
    16501 gggtagcgac tctgaacagt gatgtttcag ggtcagacag gagtagggaa ggaatacaga
    16561 gagacaataa taatggtaga tagcaagaag cgccctggca aagatctcga ccgtatcgat
    16621 cgtaacattc ttaatgagtt gcaaaaggat gggcgtattt ctaacgtcga gctttctaaa
    16681 cgtgtgggac tttccccaac gccgtgcctt gagcgtgtgc gtcggctgga aagacaaggg
    16741 tttattcagg gctatacggc gctgcttaac ccccattatc tggatgcatc acttctggta
    16801 ttcgttgaga ttactctgaa tcgtggcgca ccggatgtgt ttgaacaatt caataccgct
    16861 gtacaaaaac ttgaagaaat tcaggagtgt catttagtat ctggtgattt cgactacctg
    16921 ttgaaaacac gcgtgccgga tatgtcagct taccgtaagt tgctggggga aaccctgctg
    16981 cgtctgcctg gcgtcaatga cacacggaca tacgttgtta tggaagaagt caagcagagt
    17041 aatcgtctgg ttattaagac gcgctaacac ggaacaggtg caaaatcggc gtattttgat
    17101 tacactcctg ttaatccata cagcaacagt actggggtaa cctggtactg ttgtccgttt
    17161 tagcatcggg caggaaaagc ctgtaacctg gagagccttt cttgagccag gaatacactg
    17221 aagacaaaga agtcacattg acaaagttaa gtagcggccg ccgccttctg gaggcattgc
    17281 tgatccttat tgtcctgttt gccgtctggt tgatggctgc cttactaagc tttaaccctt
    17341 cggaccccag ctggtcgcaa acggcctggc atgaacctat ccataattta ggtgggatgc
    17401 ccggtgcgtg gttggcagat acgctgttct ttatttttgg cgtgatggct tacaccattc
    17461 ccgtcattat tgtcggcggt tgttggtttg cctggcgtca tcagtccagc gacgaataca
    17521 ttgattattt tgccgtttcg ctacgcatca ttggcgtttt ggcgctcatc cttacctcct
    17581 gtggtctggc ggcaatcaac gctgacgata tctggtattt tgcctccggt ggcgtcattg
    17641 gcagcttact aagcactacg ctacaaccac tgctacacag tagcggggga actattgcgc
    17701 tgctctgcgt ttgggcagcg ggcctgacgt tgttcaccgg ttggtcatgg gtgaccattg
    17761 ctgaaaaact cggcggctgg attttaaaca ttctcacctt cgccagtaat cgtacccgtc
    17821 gcgatgatac ctgggtcgat gaagatgagt atgaagacga cgaagagtat gaagatgaaa
    17881 atcacggcaa acagcatgaa tcacgccgtg cccgtattct tcgcggcgcg ctagcgcgtc
    17941 gtaaacggtt ggcggaaaaa ttcattaatc cgatggggcg gcaaacagac gctgcgttgt
    18001 tctccggtaa gcggatggat gatgacgaag agattaccta cactgcacgc ggtgtggctg
    18061 ccgacccgga cgacgtccta ttttcgggca atcgtgcaac gcagccagaa tatgacgaat
    18121 acgatccatt attaaacagt gcgccaatta ccgaacctgt cgctgtagca gctgctgcta
    18181 ccacggcgac acaaagctgg gctgcgccgg ttgaacctgt gactcagacg ccgcctgttg
    18241 cctctgttga tgttccacct tcgcaaccta cagtagcctg gcagcctgta ccgggtccac
    18301 aaacgggaga gccggttatt gctcctgcac cggaaggtta cccacagcag tcacaatatg
    18361 cgcagcctgc agtgcaatat aatgagccgc tgcaacaacc agtacagccg cagcagccgt
    18421 attatgcacc tgcagctgaa caacctgcgc aacagccgta ttatgcccct gcgccagaac
    18481 aaccggtggc aggtaacgcc tggcaagccg aagagcagca atccactttt gctccacagt
    18541 ctacatacca gactgagcaa acttatcagc agccagccgc tcaggagccg ttgtaccaac
    18601 agccgcaacc cgttgaacag cagcctgttg tggagcctga acccgttgta gaagagacaa
    18661 aacccgcgcg tccgccgctt tactattttg aagaagtgga agagaagcga gcccgtgaac
    18721 gtgaacaact tgcggcctgg tatcaaccga ttccagaacc ggttaaagaa ccagaaccga
    18781 tcaaatcttc gctgaaagca ccttctgttg cagcagtacc tccagtagaa gccgctgccg
    18841 ctgtttctcc gctggcatct ggcgtgaaaa aagcgacact ggcgacgggg gctgccgcaa
    18901 ccgttgccgc gccagtcttc agtctggcaa atagcggtgg accgcgtccg caggtcaaag
    18961 aggggattgg cccgcagttg ccacgaccga aacgtatccg cgtgccaact cgtcgtgaat
    19021 tggcgtctta cggtattaag ctgccctcac agcgtgcggc ggaagaaaaa gcccgtgaag
    19081 cccagcgcaa tcagtatgat tctggcgatc agtataacga tgatgaaatc gatgcgatgc
    19141 agcaggatga actggcacgt cagttcgccc agacacagca gcaacgctat ggcgaacagt
    19201 atcaacatga tgtgcccgta aacgcagaag atgcagatgc tgcggcagag gctgaactgg
    19261 ctcgtcagtt tgcgcaaact caacaacaac gttattccgg cgaacaaccg gctggggcga
    19321 atccgttctc gctggatgat tttgaatttt cgccaatgaa agcgttgctg gatgatggtc
    19381 cacacgaacc gctgtttacg ccaattgttg aacctgtaca gcagccgcaa caaccggttg
    19441 caccacagca gcagtatcag cagccacaac agccggttgc gccacagcag cagtatcagc
    19501 agccacaaca gcaggttgcg ccgcagccac aatatcagca gccgcaacaa ccggttgcgc
    19561 cacagcagca atatcagcag ccgcaacaac cagttgcgcc gcagccgcag tatcaacagc
    19621 cgcaacaacc ggttgcaccg cagccgcagt atcagcagcc acaacagcca gttgcgccac
    19681 agccgcagga taccctgctt catccgctgt tgatgcgtaa tggcgacagc cgtccgttgc
    19741 acaaaccgac gacgccactg ccttctctgg atttgctgac accgccgccg agcgaagtgg
    19801 agccggtaga tacctttgcg cttgaacaaa tggcgcgtct ggtggaagcg cgtctggctg
    19861 atttccgtat taaagccgat gtcgttaatt actctccggg gccagttatc actcgctttg
    19921 aacttaacct ggctcctggc gtaaaagcgg cgcgcatttc taacttgtca cgggaccttg
    19981 cccgttcact ttcgacggtg gcggtgcgtg tcgttgaagt tattcctggc aaaccctatg
    20041 taggtctgga gttaccaaat aaaaaacgac aaaccgttta tctgcgcgaa gttttggata
    20101 acgccaaatt ccgcgataat ccgtcgccat taaccgtggt gctgggtaaa gatatcgccg
    20161 gtgagccggt ggttgccgat ctggcgaaaa tgccgcactt gttggttgcg gggactaccg
    20221 gttccggtaa atctgtcggt gtgaacgcga tgatcctgag catgctttat aaagcacagc
    20281 cagaagatgt gcgtttcatc atgatcgacc cgaaaatgct ggagctttcg gtttatgaag
    20341 gcattccgca cctgttaacg gaagtcgtta ctgatatgaa agatgccgcc aacgcgctgc
    20401 gctggtgtgt taacgagatg gagcgtcggt ataaactgat gtctgcgctg ggtgtgcgta
    20461 atctggcggg ttataacgaa aaaattgctg aagccgatcg catgatgcgt ccgattccag
    20521 acccgtactg gaagccgggt gacagtatgg atgcccagca tccggtgctg aaaaaagaac
    20581 catacattgt cgtgttggtt gacgaatttg ccgacctgat gatgacggta ggtaaaaaag
    20641 tggaagagct gatagctcgt ctggcgcaaa aagcccgtgc cgcgggtatc cacctcgtac
    20701 tggcaactca gcgtccatcg gttgatgtta ttactggtct gattaaagcg aatattccga
    20761 cccgtatcgc ctttaccgta tccagtaaga ttgactcacg taccattctt gatcaggctg
    20821 gcgcggaatc actgctgggt atgggggata tgctctactc tgggccgaac tccacgttgc
    20881 cggtacgtgt ccatggtgct tttgttcgcg atcaggaagt tcatgccgtg gtgcaggact
    20941 ggaaagcgcg tggtcgccca cagtatgttg atggcatcac ctccgacagc gaaagcgaag
    21001 gtggtgcggg tggtttcgat ggcgctgaag aactggatcc gttgttcgat caggcggtgc
    21061 agtttgtcac tgaaaaacgc aaagcgtcaa tttctggcgt acagcgtcag ttccgcattg
    21121 gttataaccg tgcagcgcgt attatcgaac agatggaagc gcaggggatt gtcagcgaac
    21181 aggggcacaa cggtaatcgt gaagtgctgg ccccaccgcc gtttgactaa ttaatgcatt
    21241 gccggataag gcgcggtagc gtcgcatccg gcactctatc aactgaaaat tcagtatttt
    21301 cttctttcct caagctgatt attagcctgg aatagagagt agagggaact cccgatcggg
    21361 agtgacgtaa tttgaggaat aatgatgaaa aaaattgcta tcacctgtgc attactctca
    21421 agcttagtag caagcagcgt ttgggctgat gccgcaagcg atctgaaaag ccgcctggat
    21481 aaagtcagca gcttccacgc cagcttcaca caaaaagtga ctgacggtag cggcgcggcg
    21541 gtgcaggaag gtcagggcga tctgtgggtg aaacgtccaa acttattcaa ctggcatatg
    21601 acacaacctg atgaaagcat tctggtttct gacggtaaaa cactgtggtt ctataacccg
    21661 ttcgttgagc aagctacggc aacctggctg aaagatgcca ccggtaatac gccgtttatg
    21721 ctgattgccc gcaaccagtc cagcgactgg cagcagtaca atatcaaaca gaatggcgat
    21781 gactttgtcc tgacgccgaa agccagcaat ggcaatctga agcagttcac cattaacgtg
    21841 ggacgtgatg gcacaatcca tcagtttagc gcggtggagc aggacgatca gcgcagcagt
    21901 tatcagctga aatcccagca aaatggggct gtggatgcag cgaaatttac cttcaccccg
    21961 ccgcaaggcg tcacggtaga tgatcaacgt aagtagaggc acctgagtga gcaatctgtc
    22021 gctcgatttt tcggataata cttttcaacc tctggccgcg cgtatgcggc cagaaaattt
    22081 agcacagtat atcggtcagc aacatttgct ggctgcgggg aagccgttgc cgcgcgctat
    22141 cgaagccggg catttgcatt ctatgatcct ctgggggcca ccgggtaccg gcaaaacaac
    22201 cctcgctgaa gtgattgccc gctatgcgaa cgctgatgtg gaacgtattt ctgccgtcac
    22261 ctctggcgtg aaagagattc gcgaggcgat cgagcgcgcc cggcaaaacc gcaatgcagg
    22321 tcgccgcact attctttttg ttgacgaagt tcaccgtttc aacaaaagcc agcaggatgc
    22381 atttctgcca catattgaag acggcaccat cacttttatt ggcgcaacca ctgaaaaccc
    22441 gtcgtttgag cttaattcgg cactgctttc ccgtgcccgt gtttatctgt tgaaatccct
    22501 gagtacagag gatattgagc aagtactaac tcaggcgatg gaagacaaaa cccgtggcta
    22561 tggtggtcag gatattgttc tgccagatga tacacgacgt gccattgcag aactggtgaa
    22621 tggcgacgcg cgccgggcgt taaatacgct ggaaatgatg gcggatatgg ccgaagtcta
    22681 tgatagcggt aagcgggtcc tgaagcctga attactgacc gaaatcgccg gtgaacgtag
    22741 cgcccgcttt gataacaaag gcgatcgctt ttacgatcta atttccgcac tgcataagtc
    22801 ggtacgtggt agcgcacccg atgcggcgct gtactggtat gcgcgaatta ttaccgctgg
    22861 tggcgatccg ttatatgtcg cgcgtcgctg tctggcgatt gcgtctgaag acgtcggtaa
    22921 tgccgatcca cgggcgatgc aggtggcaat tgcggcctgg gattgcttta ctcgcgttgg
    22981 cccggcggaa ggtgaacgcg ccattgctca ggcgattgtt tacctggcct gcgcgccaaa
    23041 aagcaacgct gtctacactg cgtttaaagc cgcgctggcc gatgctcgcg aacgcccgga
    23101 ttatgacgtg ccggttcatt tgcgtaatgc gccgacgaaa ttaatgaagg aaatgggcta
    23161 cgggcaggaa tatcgttatg ctcatgatga agcaaacgct tatgctgccg gtgaggttta
    23221 cttcccgccg gaaatagcac aaacacgcta ttatttcccg acaaacaggg gccttgaagg
    23281 caagattggt gaaaagctcg cctggctggc tgaacaggat caaaatagcc ccataaaacg
    23341 ctaccgttaa tgttatcgtt gcggtaatgt tgttactgta tccctgtggt cgcaggctgt
    23401 ggccacatct cccatttaat tcgataagca caggataagc atgctcgatc ccaatctgct
    23461 gcgtaatgag ccagacgcag tcgctgaaaa actggcacgc cggggcttta agctggatgt
    23521 agataagctg ggcgctcttg aagagcgtcg taaagtattg caggtcaaaa cggaaaacct
    23581 gcaagcggag cgtaactccc gatcgaaatc cattggccag gcgaaagcgc gcggggaaga
    23641 tatcgagcct ttacgtctgg aagtgaacaa actgggcgaa gagctggatg cagcaaaagc
    23701 cgagctggat gctttacagg ctgaaattcg cgatatcgcg ctgaccatcc ctaacctgcc
    23761 tgcagatgaa gtgccggtag gtaaagacga aaatgacaac gttgaagtca gccgctgggg
    23821 taccccgcgt gagtttgact ttgaagttcg tgaccacgtg acgctgggtg aaatgcactc
    23881 tggcctcgac tttgcagctg cagttaagct gactggttcc cgctttgtgg taatgaaagg
    23941 gcagattgct cgcatgcacc gcgcactgtc gcagtttatg ctggatctgc ataccgaaca
    24001 gcatggctac agtgagaact atgttccgta cctggttaac caggacacgc tgtacggtac
    24061 gggtcagctg ccgaaatttg ctggcgatct gttccatact cgtccgctgg aagaagaagc
    24121 agacaccagt aactatgcgc tgatcccaac ggcagaagtt ccgctgacta acctggtgcg
    24181 cggtgaaatc atcgatgaag atgatctgcc aattaagatg accgcccaca ccccatgctt
    24241 ccgttctgaa gccggttcat atggtcgtga cacccgtggt ctgatccgta tgcaccagtt
    24301 cgacaaagtt gaaatggtgc agatcgtgcg cccagaagac tcaatggcgg cgctggaaga
    24361 gatgactggt catgcagaaa aagtcctgca gttgctgggc ctgccgtacc gtaaaatcat
    24421 cctttgcact ggcgacatgg gctttggcgc ttgcaaaact tacgacctgg aagtatggat
    24481 cccggcacag aacacctacc gtgagatctc ttcctgctcc aacgtttggg atttccaggc
    24541 acgtcgtatg caggcacgtt gccgcagcaa gtcggacaag aaaacccgtc tggttcatac
    24601 cctgaacggt tctggtctgg ctgttggtcg tacgctggtt gcagtaatgg aaaactatca
    24661 gcaggctgat ggtcgtattg aagtaccaga agttctgcgt ccgtatatga acggactgga
    24721 atatattggc taatacccaa tttttctgaa tctaaaaagc gcctttaggg cgcttttttt
    24781 gtctcccttt gataccgaac aataattact cctcacttac acgtaatact actttcgagt
    24841 gaaaatctac ctatctcttt gattttcaaa ttattctatg tatacaagcc tatatagcga
    24901 gctgctatag aaataattac acaatacggt ttgttactgg aatcaatcgt gatcaagctt
    24961 gagtgagcca ttatgaaaac gaaaatccct gatgcggtat tggctgctga ggagagtcgc
    25021 cgtggtttgg taaaaacgac agcgatcggc ggcctggcaa tggccagcag cgcattaaca
    25081 ttacctttta gtcggattgc gcacgctgtc gatagcgcca ttccaacaaa atcagacgaa
    25141 aaggttatct ggagcgcctg tacagttaac tgtggtagtc gctgcccgct acgtatgcac
    25201 gtcgtggacg gtgaaatcaa atatgtcgaa acgacaatac cggcgatgac aattacgacg
    25261 gcctacacta ggttcgcgcc tgcctgcgtg ggcgttccat gcgtcaccgt gtctacaatc
    25321 cggaccgcct gaaatatccg atgaaacgag tcggggcgcg cggtgaaggc aaattcgagc
    25381 gcattagctg ggaagaagcc tacgacatca tcgcgaccaa tatgcagcgc ctgatcaaag
    25441 agtacggcaa cgagtctatc tatctgaact atggcaccgg tacgctgggc ggcaccatga
    25501 cccgctcctg gccgccggga aataccctgg tcgcgcgcct gatgaactgc tgcggcggtt
    25561 atcttaacca ttacggtgac tactcttccg cgcaaattgc ggaaggtttg aaccatacct
    25621 acggcggctg ggcagatggt aacagcccgt cggatatcga aaacagtaaa ttggtagtgc
    25681 tgtttggtaa taacccaggc gaaacgcgaa tgagtggcgg tggggtgact tactatcttg
    25741 aacaggcacg ccagaaatct aatgctcgca tgatcatcat cgatccgcgc tataccgaca
    25801 ccggtgccgg gcgcgaagat gagtggatcc ctattcgtcc aggaacagat gccgcactgg
    25861 ttaacggtct ggcgtacgtc atgatcacta aaaacctggt ggatcaggca ttcctcgata
    25921 aatattgcgt tggctatgac gagaaaaccc tgcccgccag tgcgccgaaa aatggtcatt
    25981 ataaagctta tattctgggt gaagggccag atggcgtggc taaaacaccg gaatgggcct
    26041 cgcaaatcac cggtgttccg gcagacaaaa tcatcaaact ggctcgtgaa atcggcagta
    26101 ccaaaccggc gtttatcagc cagggatggg gcccgcagcg tcacgctaac ggtgaaatcg
    26161 caacccgtgc tatctcgatg ctggcgattc tgaccggtaa cgttggtatt aatggaggga
    26221 acagcggcgc gcgtgaaggt tcatacagct taccgtttgt ccgtatgccg accttggaaa
    26281 acccgatcca gaccagcatt tcgatgttta tgtggaccga tgccattgaa cgtggcccgg
    26341 aaatgacggc gctgcgtgat ggtgtgcgcg ggaaagataa gctggatgtg ccgatcaaaa
    26401 tgatctggaa ctatgccggt aactgcctga ttaaccagca ttctgaaatc aaccgtaccc
    26461 atgaaatcct ccaggatgat aagaagtgcg agctgattgt ggttatcgac tgccacatga
    26521 cctcatcggc gaaatatgct gacatcctgc tgcctgactg caccgcttcc gaacagatgg
    26581 actttgcact ggatgcatcc tgcgggaata tgtcttacgt gattttcaac gatcaggtga
    26641 ttaaaccgcg ctttgaatgt aagaccatct atgaaatgac cagcgaactg gcaaaacgtc
    26701 ttggcgttga gcaacagttt actgaaggcc gtacccagga agagtggatg cggcatctgt
    26761 atgcccagtc gcgggaagcg attcctgaac tgccaacgtt tgaagagttc cgcaagcagg
    26821 ggatctttaa aaagcgcgac ccacaagggc atcacgttgc ttataaagcc ttccgtgaag
    26881 atccgcaggc aaacccactg actacgccat cgggcaaaat tgagatttat tcgcaggcgc
    26941 tggctgacat tgccgctacc tgggaattgc cagaaggcga tgtgatcgat ccactgccga
    27001 tctacacgcc gggctttgaa agttatcagg atccgctgaa caaacagtat ccgctgcagc
    27061 ttacaggttt ccactataaa tctcgcgttc actcaactta cggcaacgtt gatgtgctga
    27121 aagcggcttg ccgtcaggaa atgtggatca acccgcttga tgcccaaaaa cgcggtatcc
    27181 acaacggcga taaagtcagg atctttaacg atcgtggtga ggttcatatt gaggcgaaag
    27241 tgacgccacg aatgatgccg ggcgtggtcg cactgggtga aggcgcctgg tatgacccgg
    27301 atgcaaaacg tgtcgataag ggcggttgta tcaacgtact gaccactcaa cgtccgtctc
    27361 ctctcgctaa ggggaatccg tcacatacaa accttgttca ggttgaaaag gtgtaaggag
    27421 taaccgatga caacccagta tggatttttt attgattcca gccgttgcac cggttgcaaa
    27481 acctgcgagc tggcttgtaa agactacaaa gatttgacgc cagaagtcag tttccgccgc
    27541 atttatgaat atgctggcgg cgactggcag gaagataacg gtgtctggca ccagaacgtg
    27601 tttgcctact atctgtcgat ttcgtgcaac cactgcgaag atccggcttg tactaaagtc
    27661 tgcccgagcg gtgcgatgca taaacgcgaa gatggttttg ttgtggtcga tgaagatgta
    27721 tgcattggct gccgttactg tcatatggct tgcccgtatg gcgcaccgca atataacgaa
    27781 acgaaaggcc atatgaccaa atgcgatggt tgttatgacc gtgttgcgga gggtaaaaag
    27841 ccgatctgtg ttgaatcttg cccgctgcgg gcgctggatt tcggccctat cgacgagctg
    27901 cgtaaaaaac atggcgatct ggcggccgtt gcgccgttgc cgcgagctca ctttaccaaa
    27961 ccgaatattg tgatcaaacc caatgccaat agccgcccga ccggggatac cactggctat
    28021 ctggcaaacc cgaaggaggt gtgagatggg aagtggatgg catgaatggc cgctgatgat
    28081 cttcacggtc ttcgggcaat gtgtggcggg tggttttatc gttctggctt tggcgctgct
    28141 caaaggcgac ctgcgagcag aagcccagca gcgtgttatc gcctgcatgt ttggcttatg
    28201 ggtactgatg ggcattggct ttatcgcctc tatgctccat cttggttcac cgatgcgcgc
    28261 ctttaactcg ctcaaccggg taggggcttc agcactcagt aacgaaatcg ccagcggttc
    28321 gatctttttt gctgtaggcg gcatcggctg gttgctggca atgctgaaaa agctgtcacc
    28381 ggcattgcgt acgctatggc tggtggtgac gatggttctt ggcgtcgtct ttgtctggat
    28441 gatggtgcgt gtgtataaca gcattgatac cgttccgacc tggtacagca tctggacgcc
    28501 gatgggctgg gcgatgcgtc tgctgccagc catttctgta ctggcactgg tggtgagtgg
    28561 cgtgatgtca gtgatgcagg gcacagagct ggcaactatt catagttctg tgcagcaggc
    28621 cgcagcgctg gtgccggact atggtgcgct gatgtcctgg cggatcgtgc ttttggccgt
    28681 tgccctgtgc ttgtggattg caccacagct aaaaggttat cagcctgcgg ttccgctact
    28741 atccgtctca ttcattctgc ttctggcagg ggaattaatc ggtcgcggcg tattctatgg
    28801 tttgcatatg accgtgggga tggccgtcgc aagctaatca gtaacaaccg gggtttcggc
    28861 cccggttctc ttttatttct gcttcgttaa cgtgtcataa ctggtcatca aattacgata
    28921 atcaggaata tggttagaga acaacgtcgc cagtccttca atatcattac gccagtcacg
    28981 gtgcagctcg caagccacgc cgaaccatgt catcaattgc gcaccagctt gcgacatgcg
    29041 atcccacgcc gaatgccggg taatttcatt aaaggtccct gatgcatcag tcacgacaaa
    29101 gacatcaaac ccttcttcaa tggctgaaag cgccgggaat gccacgcaaa cttcggttac
    29161 cacaccggca ataattaact gttttttacc tgtcgctttg actgctttta caaaatcttc
    29221 gttatcccag gcgttaatat tgcccgggcg agcaatgtaa ggtgcatcgg ggaattgtgc
    29281 tttcagttct agaactaatg ggccgttagg accggtttca aaactggtgg taagaatagt
    29341 tggtaagttg aaatacttag ccaggtcacc cagcgccagc acgttatttt taaacttatc
    29401 gggttcgata tcccgtacaa gggaaagtaa accagcctgg tgatcaacaa gcaaaacggc
    29461 agcatcattt ttatcaagac gaacatacgg tttggtcatc ctcttctcct ttcgagagtc
    29521 agtggtacgt ctgaaatcgg tcagacgcga tgcattgctc tgaaagcata gacgggaaat
    29581 atgagtttgc tgtgaccatg aaatttttcg actgaacgaa gcgtatgaaa tttgtgttag
    29641 atcaataaaa acaatcagat gagattaatc atctcttcag cattagcgtt atgaatgagt
    29701 aatataaatc gataataccg ccgctgcgat atgcgcgtat tttcacccat tgacaatgtt
    29761 tttggcggtg gcatgatgcg catgaaattt gaacttcctc acggttttaa ttcatgtcca
    29821 cgtatacccg gcctgtcatg cttttgctgt ctggcctgct tttgttgact ctggcgatcg
    29881 cggtgttaaa tacactcgtg ccgctttggc tcgcccagga acacatgtcc acatggcagg
    29941 taggcgttgt cagctcatcc tattttaccg gcaaccttgt cggtacattg ctgacagggt
    30001 atgtcattaa acgcattggc tttaaccgca gctattatct gtcctccttc atttttgctg
    30061 ctggctgtgc cggccttggc ctgatgattg gtttctggag ctggttggcg tggcgttttg
    30121 tcgcgggcgt cggctgtgcc atgatttggg tggttgttga gagcgcgctg atgtgcagtg
    30181 ggacgtcacg taaccgtggg cgtttgcttg ctgcgtatat gatggtttat tacgtgggaa
    30241 cgtttttagg ccagttactg gtcagcaaag tctcaaccga gctgatgagc gtattgccgt
    30301 gggttacagg tttgacattg gcagggatct taccgctgtt gtttacgcgt gtcctgaatc
    30361 agcaggctga aaaccatgat tcgacgtcaa ttacggcaat gctaaaactc cgtcaggcgc
    30421 ggcttggcgt gaatggctgc attatctcag gaatcgttct gggatctcta tatggcctga
    30481 tgcctctgta cctcaatcac aaaggggtga gcaatgccag tattggtttc tggatggcgg
    30541 tactggtcag tgcgggtatc ctcggacaat ggccgattgg acgtctggcg gataagtttg
    30601 gtcgactgct ggttttgcgt gttcaggtct ttgtcgtcat tctcggcagt attgcgatgc
    30661 ttagccaggc ggcgatggcc ccagcgttat tcatcctcgg tgccgctggc tttacgctat
    30721 atccggtggc gatggcctgg gcttgtgaga aagttgaaca tcatcaactg gtggcgatga
    30781 accaagcctt actgttgagc tataccgtgg gaagtctgct tggcccgtca tttagcgcta
    30841 tgctaatgca gaatttctcc gataatttat tgtttatcat gatcgccagc gtatcgttta
    30901 tctatttgct gatgctgctt cgcaacgccg gtcatacgcc gaaacccgtt gctcacgtgt
    30961 aaatgaattc aagcaaagtg tgaacttact gtttcacact ctgctttttt gtttcttcta
    31021 tctgacttgc ttttttccaa attttattcg tttaaaaata aaatgtgcag caggttataa
    31081 ttttgcattt cgctatttcc gcacttctta tttgccgcgc ataatccctc gttttacaga
    31141 tgccccttta attttggcga aggatttgtc tatggctggg aatgttcag
//
LOCUS       DAJTRD010000040        30295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTRD010000040 DAJTRD010000000
VERSION     DAJTRD010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.040"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OO315_002036"
     CDS             complement(<1..815)
                     /gene="wcaD"
                     /locus_tag="OO315_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5890957.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYILIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLG"
     gene            complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OO315_002037"
     CDS             complement(790..2007)
                     /gene="wcaC"
                     /locus_tag="OO315_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5890958.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OO315_002038"
     CDS             complement(2004..2492)
                     /gene="wcaB"
                     /locus_tag="OO315_002038"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5890959.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OO315_002039"
                     /pseudo
     CDS             complement(2495..3334)
                     /gene="wcaA"
                     /locus_tag="OO315_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OO315_002040"
     CDS             complement(3427..5589)
                     /gene="wzc"
                     /locus_tag="OO315_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5890960.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OO315_002041"
     CDS             complement(5592..6035)
                     /gene="wzb"
                     /locus_tag="OO315_002041"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5890961.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OO315_002042"
     CDS             complement(6041..7180)
                     /gene="wza"
                     /locus_tag="OO315_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5890962.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7489..7638
                     /locus_tag="OO315_002043"
     CDS             7489..7638
                     /locus_tag="OO315_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890963.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7839..9422
                     /locus_tag="OO315_002044"
     CDS             7839..9422
                     /locus_tag="OO315_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5890964.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OO315_002045"
     CDS             complement(9874..11727)
                     /gene="asmA"
                     /locus_tag="OO315_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5890965.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OO315_002046"
     CDS             complement(11749..12330)
                     /gene="dcd"
                     /locus_tag="OO315_002046"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5890966.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OO315_002047"
     CDS             complement(12422..13063)
                     /gene="udk"
                     /locus_tag="OO315_002047"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5890967.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13381..13623
                     /locus_tag="OO315_002048"
                     /pseudo
     CDS             13381..13623
                     /locus_tag="OO315_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13622..14563
                     /gene="mdtA"
                     /locus_tag="OO315_002049"
                     /pseudo
     CDS             13622..14563
                     /gene="mdtA"
                     /locus_tag="OO315_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14563..17685
                     /gene="mdtB"
                     /locus_tag="OO315_002050"
                     /pseudo
     CDS             14563..17685
                     /gene="mdtB"
                     /locus_tag="OO315_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17686..20751
                     /gene="mdtC"
                     /locus_tag="OO315_002051"
     CDS             17686..20751
                     /gene="mdtC"
                     /locus_tag="OO315_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5890968.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20752..22167
                     /locus_tag="OO315_002052"
     CDS             20752..22167
                     /locus_tag="OO315_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5890969.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22164..23567
                     /gene="baeS"
                     /locus_tag="OO315_002053"
     CDS             22164..23567
                     /gene="baeS"
                     /locus_tag="OO315_002053"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5890970.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23564..24286
                     /gene="baeR"
                     /locus_tag="OO315_002054"
     CDS             23564..24286
                     /gene="baeR"
                     /locus_tag="OO315_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5890971.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            24477..24809
                     /locus_tag="OO315_002055"
     CDS             24477..24809
                     /locus_tag="OO315_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5890972.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24956..26317
                     /gene="yegQ"
                     /locus_tag="OO315_002056"
     CDS             24956..26317
                     /gene="yegQ"
                     /locus_tag="OO315_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5890973.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26659..26976)
                     /locus_tag="OO315_002057"
     CDS             complement(26659..26976)
                     /locus_tag="OO315_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890974.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27382..28281
                     /gene="yegS"
                     /locus_tag="OO315_002058"
     CDS             27382..28281
                     /gene="yegS"
                     /locus_tag="OO315_002058"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5890975.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28363..29136)
                     /locus_tag="OO315_002059"
     CDS             complement(28363..29136)
                     /locus_tag="OO315_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5890976.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="OO315_002060"
     CDS             complement(29242..30282)
                     /gene="gatD"
                     /locus_tag="OO315_002060"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5890977.1"
                     /translation="MKSVVNDTDGIVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMQGKVLLIP"
BASE COUNT         7012 a         8047 c         7828 g         7408 t
ORIGIN      
        1 cctaaatcgc ccagacgggt ggaaatataa ggaaacgcga ttaccacacc aacccggaat
       61 acagccagag aaatcaatgc taacggtaat tttttcttaa tcgcctcttt attcagatat
      121 tgaaatgccc actccagcaa ataaaacagg ataaaggtca taacccctga aaacgatccg
      181 gataatatta tccctgcgag aatcagggca tctgttttag gcgttttgat accaaactgt
      241 ttgatgctga gccaaattga gattaatgcc agagcgaaaa atgccggttc gaaataaagt
      301 gctgtcgtgc gcttgccgcc gaatttaatg aaattcagca catagctgtt gctgtaaatc
      361 agatatttcg aaatcgactc cataatactg ctgccaccgg tgaggataat ttgcgccatc
      421 tccaccgccg ccagcgccac caccagtccg accaccagat aaaagaaacg taatatctta
      481 cgatggttgt gtggcgaaat tgttttaaaa cgaatactcc acaccatgcc aataatgatc
      541 aaaatataga caaacagcat agttgaagtg acgtatttac cggcatccaa cgactgacca
      601 aacaggtagt taaacgccgt gagtcccgcg ccaatcccta aggcaatcat caatttttta
      661 atgctgatgc gttctaaaaa cagcagtaac aagacaggta aaaaagtgac gatggtgatg
      721 gggaaacttt cgccaagctg ggcgattttg acgttaacca gcaggtagat cagtggcagc
      781 agcaggtagc tacagattct gatagaagtt gacatactcc tccagcatct gttgtccact
      841 gtaggcggcg cgactgcgtt ggctgaactc agccagcgtg gtaccaaata tcgcctgcgc
      901 gatttccggt ttgcttaact gtaccagttg cagcacctct tcttcactga cggttttacc
      961 accggatttt tgcagcactt cccgcgccgc atcgctatgg gtggcaatca ccggcacgcc
     1021 aatcgatagc gcctcacaca aaatcagcgg gtagttatcg acacgagaac tgaacaccag
     1081 cgcatccatc tgattgagcg cgctcatcaa cttgcgcttg tcagtttcaa agccgtgatt
     1141 aaccacgttg ccagcggtga acggcgagaa cttaccaaag gtatgcagtt cgattttgtc
     1201 gcccagcgcc atcatctcac gcaccagttg ctggttagtt ttgccgtcgt aacgcaggtc
     1261 atgcgccacc actgcgattt tcggcttgcc ctgagtttcg cgcaccggag gcagatctgc
     1321 cagaatcgct tcggttgcca tatcaatgcc attattgata atccggcaac gccctggccc
     1381 gtacagacta ttgaaagcgt cagccacatg ctggctgggg gaaataaact gacagcccag
     1441 cgccagcatc tcgcggaata actggcgttt gcccgccacc agctggtgcg cgcgatcaat
     1501 cttcaccggc ggatagttat ttaaggtcgg gcatttctgg cagcccgttt tccagccttc
     1561 gcaaccgtcg gtaaaggcgc agcgtccggt tacactccag tggtcgtgca gcgtccagac
     1621 cagggtgaca tccggtttat ggtttttcac tttttcgcaa aagcgcacca cgctgttaag
     1681 attcaaccag tagctgtgca gcacatgaaa atgcaggacc accggacccg gtgtgcgagt
     1741 aatggtgcga tataactcat tgaaattgcc gaacagatcg cgattaaaca gacgaaacaa
     1801 cgcaatgttc gccatcgcgg tcatccgcgg cgtatgtttg atgacctgcg gatagttctg
     1861 atggctgacg ctctctttgc cgcctttgcc gtagccgtag acaaaatgtg acgccagccc
     1921 ctgttgcagc gcacgctggt ggagatctaa cgccacacct gctgccccgc cttccgccag
     1981 tcgcacatta aattgcagaa tattcattta atcaccttca ctcgcgcttt ttctcccacc
     2041 accagcgcgt tgtccgggac agagtcgagc accacgctgc ccgcacccac ggtgacgttg
     2101 ttaccaagcg tgatatcacc aagaataatg acgttggcac cgagttcgac gccgttgcca
     2161 atgtgtggac atgccatgtt atcggcaccc cgattgccga tagtgacgcc gtggcgaatg
     2221 gtgaaatcat cccccgccac cacgttttta ttgatcacta cggcgtaacc gtgatggata
     2281 gtaaagcggc ggccaatggt cgcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2341 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2401 cgccacaccg aacaaaaatg agcgacgcga taggcaagta ccatgcagca cgggcgtaaa
     2461 ctccagctgt tggcgcgcag atcttccagc atctttagcg cccccgaata ccatcagcca
     2521 gacgtttgcc gttacgcacc gacagcagcg tcaacaaggt gcgccaggtc atacgtttat
     2581 tgcggatctg gtagagggta aacagctgat attttttact ggcgcggtcg aatttgtctt
     2641 tgtgcttgcg gtaaaagtgg aagtatccgg agaatttttt cggtgacgag gtgatctgca
     2701 tttcgccgtg attgatatgc aggatctgcg tcgcctcttc cactttccac ggttcgccgt
     2761 actccaccac catccgcagg aatatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2821 acaaacactc tttgaaacgc catgcccagg taaagacctg attgccaatg atattacgtt
     2881 tatagaacag gcggcgtgag tacggcgatt tgggatacag tggtaagctc gccggttggg
     2941 aataaacttc tccctagcaa acgtagtcgt tagcgtacaa aaaggcgtgt gtgaccagtt
     3001 gctgtttatg ggcgaggaag acgctcaggc ggttgggcgt ccattcatca tcatcatcga
     3061 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgcacc gcgcacgccc
     3121 cgctgttaat gtcgttgtgg atgtaagtga tacgcggatc gttgagggca gtgacgtact
     3181 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3241 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggttg
     3301 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccggc ttatgagctg
     3361 gtttgcttgc cggatgcggc gtgagcgcct tatccggcct acggggcggt gcgaatgcag
     3421 gcgtcgttat ttcgcatccg acttatattc gtattcgtaa tagccataat cctgatacgc
     3481 gctggcgcgg cggaagatgg agttcagaat cacccctttc accggaatac cgttttgctc
     3541 aaagcggctc agactggttt ccacttcttt caatgtgttg accgcataac gcgccaccat
     3601 taacgtggtt ccgacatgac gaccaacaat tgccgcatcg gtcactgcca gaatcggcgg
     3661 cgtatcaatc aacaccaggt catagttttt actcgcccag ttcaccagtt cggcaaagcg
     3721 ttcgctcatt aacagttcag aaggatttgg cggcacctga ccgcgcggga tcaggtcaaa
     3781 tttggcaata gaggtcggtt tagcagcggt agtaatatcg ccctgaccaa tcagaatctc
     3841 cgacagccca ttgacattat tggtgcccaa cagctcgtgg gtgtagcctt tgcgcatatc
     3901 gcagtcgatc aacaacacgc gtttattggt ctggctgatc accgccgcca ggttggcgca
     3961 gacaaaggtt ttaccgattg acgggctaac cccggtcatc atcaacacat tgttctgcgc
     4021 ctgcatcatc gcgaagtgca gactggtacg cagactacgg atggcttcaa tcgccagatc
     4081 ggttggattc cccactgcca gtagctggct ctgtttatag cgtttaaccc ctttgatggt
     4141 tttgacgcta tcgcgcgctt tctgccactc cgacagcggg atgctggcat agacgctgat
     4201 tccgtgttct tccagcactt gcgggctttc gatgccgcga ttaaacaacg agcgcagcag
     4261 cacacccacg atagagagca tcaggccaag gataatcgcg ccgaggataa tcagcccttt
     4321 cttcggtttt agcacgccag gctgggtgat tgccgggtca ataatgcgca catcgccgac
     4381 ggtgctggct tcggtgattt tcagctcctg ctctttattc agcagttgca tatagacctg
     4441 ctgaccagac tcgacatcgc gggtcagacg gacaatttcc tgctggtttt tcggcatcgc
     4501 cgttacgcga ccgttaagtt tggctttctc ttcttccagc gcctgacgtt tctccagcag
     4561 cgtgcggtac gccgggtgaa ctttggtgta cagcttggag atttccgcct ctttaaaggt
     4621 cagttcgttc aactgcgcgt cgatgttcac catcgaatcg agcaccgctt tcgcttcaag
     4681 cggcagatca acagaatctt tatcctgacg gaaggcattc agtttgtttt cggcaacatc
     4741 gaggcggcta cgtacttccg gtaactgttg cgcgaggaaa gcgaggcttt tcgacgcttc
     4801 cgccgatttg cgctcaatat tttgttcctg atagttacga gcgatgctgt taagaatgtc
     4861 gcggatctgt tcgcgatctt caccggtata agtcaggctc agtacgcctg cgtctttgcc
     4921 gttctccgtt accgtcaggc tgttttgcag ttggttgatc atccccaacg tggagtattt
     4981 ggtgacggta aactcactgc ccgggcgggc gtgaatggct tcaaccatca gcgtgacgcc
     5041 ttcttttttc agcatctggc ccgcttgccc acgggcgcta aagccgccat cactgctcag
     5101 ggtgtagttt ttgtcatcca gcacattaag cgtaaacacc tgatccgcca tctctttcgg
     5161 gcggttaaag gtagtcactt tcaccgtctc gttctgacgc cccatcaggc gatcccagcc
     5221 cgccccgaaa atcgggaacg tgtttttgct caccgcaata tcgaggtcga gatcgtccac
     5281 cgttttacca agcaccagac gcgaacgaat caactggatc tcggcgtccg atgcaggcgg
     5341 tttgttggct aatgccgagc cgatatcctg caccagcgaa ttgccgctgt tttgctcgat
     5401 ttgtaccagt gcgtcggcac tataaatcgg cgtggcgaag aaagtgtaaa ccacggcaca
     5461 gagggcaaat acgacggtga tgccaatcac ccaccagcgc gcttcaatga cggtgccgac
     5521 caggcgacca atatcgattt catcactgcc cgttaccgga gcggcatgtt gttttacttt
     5581 ttctgtcatt cttatacctg ctctgcgttc aatgcctgcg cccactggcg ggcagaccgt
     5641 tcaagtaatg tgtacaccgc tgcaaacgtt tcccggcttt tgtgatacgg atcggggatt
     5701 tcacattcgt tatcccagtg accaaacagc atcactttgc cgcgcatctc cggtgccatc
     5761 tcgcataagc gttcgatatg gcgcttatcc atggtcaaaa tcaggtcata gttgcgacac
     5821 aggcggcggc tgatttgtcg tgcacagtgg ccttccagag acagttgatg ttctgcggcg
     5881 acgctgatag cggtaggatc agcgccctta ccgaccagcg cgccgagtcc agcggactcc
     5941 actttcagtt ccgggtgata acgttgcagt aagcgttccg ccatcgggga acggcaaata
     6001 ttgccgacac agacaactaa gatgttgtta aacatgacga ttaccagtta tgaatgtcgc
     6061 tggctgtatc cgtcatgtaa cggacaccgc taatagttgg cagcaactga ttgatcagac
     6121 ggttccagcg ggaaaccggg gcggtggtga catacaccac gtcataaggt tgcaggcgga
     6181 attctgtcgc catcaccagc gacgtcgcat cggacatatc cagctggtag atattggcaa
     6241 tcttgccgtt acgcccgccc tcgcctttca gcggacgaat gacaaagatg ccgctggcgt
     6301 tggaggtggt catgtcgata ccttcggcat tggccagagc ttcagtcagg gtcatgccgc
     6361 taaagtccat tttcagggtg ctctgtttct tcacttcacc catcacaaat actttcagat
     6421 catcattacg cggcacgtag agaatatcgc cgggataaag cagtcggttc tgattgaggt
     6481 cgccgttttg catcagcgcc tgtaaagaaa tgcgttcttc acgaccattg tgtgttagca
     6541 ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc ggcgttgatg gcgtcgagaa
     6601 tggtcagtgg tacgttggtg atcgcctgtt gaccggattt attcacctga cctgagatat
     6661 aggccttttg tgagcggaag gcggcgatat taacgtccac ctgcgggtca gcgatgtacg
     6721 tcgctaagcg cccggtaata tcactgcgga tttcagcgag cgttttcccg actacgtgaa
     6781 ccttgccgat atacgggtaa aacatagtgc cgtcaggctg tacccagttg ccggtgtcgc
     6841 tggagctgcg gtactgacca gctggcgtgg tgagttccgg gtgatcccag acggtgacat
     6901 taagaacgtc ccccggcccg acgcgatact ggtaattcgc gatctcgctt tccagcgtca
     6961 tattggggcg cgctacattc gggcgtgggc gtaattggtc aatcaggcgc ggggtcagcg
     7021 gataaacatt caccattttg tcgagatcga aatcagcgtc ctgctgtttg atgacgtctt
     7081 tgcccatcgt cgacatattg ctgcccggaa gtactgtgca accgcttatc aaggttactg
     7141 acaccaataa tggcatcaat ttcattttgg atttcatcat tgtttattta tcactttggc
     7201 agagtaatta tcctgtgcac tattaatagc aatgtcgcca tgcacattta ccttgcagtt
     7261 aattgaataa aaatttaact ggcatcagtc ctaaaaaaat tgatttcatc cgcaggctat
     7321 tgacagaata attcagactg gtctttcagg catccagaca cgctaccgcc cctggctttt
     7381 tagctaccaa tacactgatt gagcttaatt tttcaaaccc tctctgcata caatcgttga
     7441 tgagaaaggg ttattgcgga aattaacttc cgaatataag gtgacattat ggtaattgaa
     7501 tattggctcg ccaataatgc aggaggaagt gttacagcta acggaatagc aggcaaggta
     7561 acaattcggc aattggctat ttttaagaat tatattaagt atcattcaat atggttttta
     7621 ggagtttctt taggttgaca atatttaata ttgcgtctcc acatgcgata tttcttaaat
     7681 aatgttttat tattaccgct tttaattcag ggataatgtg aggttattac cctcaaataa
     7741 tatgagataa atagtgctgc aacattgcat ttttgccccg atttatccat gatcgaattg
     7801 tgacatttgt catacaacga ataggttttg tacttactat ggaatggatt gccgatccgt
     7861 ctatctgggc cgggttaatc acgctgattg tgatcgaact ggtcctcggc attgataacc
     7921 tggtctttat tgccatcctc gccgaaaaac taccgccgaa gcagcgcgac cgcgcacggg
     7981 ttaccgggct gctgctggcg atgttaatgc gcctgttact gctagcgtca atctcctggc
     8041 tggtcaccct gactcaaccg ctgttcagct tccgctcgtt tacctttagc gcccgcgact
     8101 taatcatgct gtttggtggt ttcttcctgt tgttcaaagc cacgatggag ctgaacgaac
     8161 ggctggaagg gaaagacagc aataatccca cacaacgcaa aggtgcgaag ttctggggcg
     8221 tggtgacgca aattgtggta ctggacgcca ttttctcact cgactcagtg attaccgccg
     8281 tcgggatggt cgaccattta ctggtcatga tggccgccgt ggttatcgct atcagcctga
     8341 tgttgatggc cagcaagccg ttaacgcaat ttgttaacag tcacccgacc atcgttattc
     8401 tctgcttaag cttcctgttg atgattggct ttagcctggt ggcagaaggt ttcggcttcg
     8461 tcattccgaa aggctacctg tacgctgcca ttggtttctc ggtgatgatc gaggcactca
     8521 atcagttggc tatctttaac cggcgacgtt ttctttccgc taatcagacg ctacgccagc
     8581 ggactaccga agcggtaatg cgcctgctta gcgggcaaaa agaagatgcg gaactggacg
     8641 ccaaaaccgc gtccatgctg gtggatcatg gaaaccagca gatctttaat ccgcaggaac
     8701 ggcggatgat tgagcgggta cttaatctta accagcgtac cgtaagcagc attatgacgt
     8761 cgcgccacga tattgagcat atcgatctca acgcgccgga agaagaaatc cgccagttgc
     8821 tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg tgatgacgca gaagatttgc
     8881 tcggtgttgt tcacgttatc gacctgctac aacagtcact gcgcggcgaa ccgctcaacc
     8941 tgcgggtgtt gattcgccag ccgctggtgt tcccggaaac cttgccgttg ttaccagccc
     9001 tggagcagtt ccgtaatgcc cgcacgcact ttgcttttgt ggtggatgag tttggctcgg
     9061 tggaagggat tgtgacatta agtgacgtca ctgaaaccat tgccggtaac ttaccgaacg
     9121 aagtggaaga gatcgacgcc cgccatgata ttcagaagaa tgcggacggt tcctggacgg
     9181 cgaatggtca tatgccgctg gaggatctgg tgcaatatgt gccgctgccg ctggatgaaa
     9241 aacgtgaata tcacaccatt gccgggctgt tgatggagta tttgcagcgt attccaaaac
     9301 ctggcgaaga agttcaggtg ggggattatt tgcttaaaac gttgcaggtg gaaagccatc
     9361 gcgtgcagaa ggtgcagatt ataccgctgc gtaaggatgg cgagatggag tacgaggtgt
     9421 gatggtatcg gccattttac ccaatgctgc gtaacgcctt ataacttcag caatgaagcg
     9481 ttgtctgtgg taaacggaca tcacagcggg ggtgtgggtc cggctgtaaa tcgccgaatc
     9541 gtttcctgta ggctggggca gcccacatgg atgtgggctg agggcgcgtt ttacagggat
     9601 gttacctcgc gcccgtaccc gatagccgaa aggaataaga tgagggaacc gcgccagcgg
     9661 cgatttaccg ccgggagccc gggttgccag ggtggtggcg attgagccac cctggcacgt
     9721 tcaccggctt gagcgttcca tagtagcgag gaacatcaag tgaacggaac agccttcgat
     9781 cttacatatt cagaatataa tagtgccgcc gcgaacgcct gatgcgacgc tttacgcgtc
     9841 ttatcaggcc tacaggacag gtcacctggt aacctacatc ttctccagca acttcttcac
     9901 atctttgcca ttgcgggaat ctttattccg ctcggcccag tcattcaggc gacgtttcgc
     9961 ttcgtcctgt agatgtttgc gcagtaactg atccacttgc aggctgtaat tgagttgctg
    10021 ccagttgcca taaacccgca gcggtactgg cgtttctttg aggaaatcaa tcagtttgct
    10081 ttccccgttc cagccaccca cgacccgaat atcaaactgg gtgtcgcagg tttgatctgc
    10141 cagattcaac atgccttccc ctgtcagcgc cagcacaggc gattgacctt gcatgtcgtt
    10201 taacgtcacg acgccatcct tcaacgtcaa atcggtggta aagcggtcaa gccgcgtcac
    10261 gttatcgaag ttttcagcgg ccttcacatc accgccatta cgctctaccg cttgctgaat
    10321 catctgctgg aagttcatcc cttccatacg tgtgtcggtc atctcaacat gcgcctgtcc
    10381 ttgccagttg tggcggaatg cgtcggcatc tatgtcagca ccggagaaat caccagccag
    10441 tgacattttt ccggtcaacg aaatcggata gttaaacgcc ttcagaatgg taccaatctc
    10501 aacgttctcc agccgtggct ggaagtttat ccgcggattt attgatgtcg cgtccagcgt
    10561 gcccggcagt gaaacctttc caccgttaag cttgccctgc agttgagtaa tttccagcaa
    10621 acccgacttg ttggtcattt gcgtggcaac atctgtaaaa ttcattccgc gccagcgcac
    10681 gttactggcc tgcaacaaaa tatcagctgt aaagccttgc agtccctgat aggccggttc
    10741 atcaatacgc gaagaaatga ccgggcgcgg caacgttgat tggctctgcc cctgctgcgc
    10801 ggcaccgttt tcaccattcg cggtttcatt aagcgggatg aggttgtcaa gattcagttg
    10861 cgggaattgc agtctcagct gccattccgg tttctctgtc agcgtgacct gtgcttgtcc
    10921 gctcagcgta ctgtcattgg cggtcaaact aatttggtta aatgaaaggc gtttatgtga
    10981 ctcctgccac tgggactgga aactcccctg cccctgaata ccttgttttg gtaaatcggc
    11041 accctgcaac tgccagttaa tttgttcaat agccgctgtt aaatcatgcg gataatcaga
    11101 agcatctacc gtaccgttaa gggatatcgt gagatcgcgc tgatcgcgat taacccgccc
    11161 ggagaactca aatgagccac gatgttgggg atcttgttcc atttgcaggc ggatattgcg
    11221 gattgtcacc tgctcgtcat cttcatgctg gaacaccaga acgctgtccg ccaccttaag
    11281 actggatata tcaaacgacc atccgcgatc gtctgacaga tccggcaagg tattgtcgcg
    11341 cggtgcaacc ggagcgtctt cactgcgcac cgcttccgtc tgcggcgtca gttggatcac
    11401 tgcccctttt agcatcacct gcttaacgct cagttgatga ctcagtagtg gtaaaagcgc
    11461 cacgtccaga cgcatgttgt cggcgcgaac cagtggctgg cttgcgccct gggcggtgag
    11521 agacattcgc ccggagagga tactaagctg cggccagacg tgccaacgca gtggcccgtc
    11581 gagctgcaat tgataaccgc tacgtgcagc aacttgcttg accatatagt cgcggaaatc
    11641 attcggattc accagcaaca ctaacgcaga taacccggcc accagcacga ccaagagtat
    11701 catcagcgtc gtcagaaatc gtctcatggt atcctcaatg ggcctgaatt agtctttatc
    11761 gattcggctg gctaccgcgc cctgctggtt gcgatatttc gcatcttcac ggcggttgta
    11821 aggtcgcgcc gccgggccgg aaagcggctc aaagctcagc gcaccaatta acatgcccgg
    11881 acgcagcgcc agcggcagct taccggagtt gtagaactcc agcacaatgc aaccagacca
    11941 gcccggatcg atacggtgcg cggtgacgtg caccatcagc cccagacgcg ccaaagagga
    12001 acgcccgtcc agccagccca ccagatcggc tggcagcgtc accgactcca gtgtcaccgc
    12061 cagcgccagc tctcctgggt gaagatagaa cgcctcgccc tcgtcgagaa cgatctcatc
    12121 gctcatcacg cggtcaagcg cggcgctcac ttcatctttg ggaccgctca gatcgataaa
    12181 cgctgccgtg tgaccacgga aggtacgaaa tttattgccc aggcgtacat ccaccgtcgc
    12241 gccgttaata cgctccactg gcggacgtgg gttgatcgac aaacggcctt catcaagcca
    12301 ggcttcaata tctcggtcac acagacgcat ggcattttct cctttcgcac atcactccct
    12361 taacgccaat tacgtcaagg gcataccaac ttatcactga acggtacaca atttatcaag
    12421 cttattcaaa gaactgactt attttcgctt tcaatatatc gatcgcgatg cggtttttcc
    12481 cgccgcgcgg cacgataatg tccgcatatt gtttagaagg ctcaatgaat tgcaggaaca
    12541 tcgggcgcac ggttttttga tattgcgcca tcacggaatc cattgaacgc ccacgctcgt
    12601 taacgtcacg cttgatgcgg cgcatcaggc agatatccag cggggtatca acgaaaatgg
    12661 agaagttaag ttcgtcacgc aaacgcgcat ccgtcagcaa caaaatgcct tcgagaatga
    12721 tgaccttctt cggctcaacc gtcaccgttt ctttcatacg cgtatgttca acatagctgt
    12781 aaaccggcag gtcaattgcc gagccgcgtt tcaacgcttg taaatgctca agcaacaggc
    12841 tgtgatccat cgcgctggga tggtcgtagt tggtcttaac gcgttcttcc atcgacagat
    12901 ggctttgatc tttgtaatag cagtcttcgg gaattacgcc gatgtgttca tcaccgactt
    12961 gctcacgtaa ttcacgataa agggtactgg caataagact cttgccggaa gccgatgcgc
    13021 cagcgatacc gataatgacg cactgatgag actgatcagt catatattta gcgacctgat
    13081 taacctggat gttaggaagg gggcgacgaa gcgccaaacg cggcaattat agggatttca
    13141 tccgtctgat accagtcgaa tagcgttgcc gcgcgctcag agttaattgt tgacaaagaa
    13201 ttcccggtgg caaattacgt tgatcagttt tatgtaaggt aaaaaatgtt atacgcagtt
    13261 gcgcaaatta tccgccttta cgtcacttta tgagcaattc gcatataaaa tgtaaaactt
    13321 ttgtactagc ataaacacag aaacgaatac tggcgacctg gccttgcgga taaagcggta
    13381 ataagcaaac aatcacagca tgtattaatt gccctgcccc acccgctgct tcacctggtc
    13441 agtttaggtt tagtctcgtt tatctttacc cttgtctcgc ttgagctttc gcagtttggc
    13501 acccaactcg ccccactgtg gttcccgacg tccatcatga tggtggcgtt ttatcgccat
    13561 gccgggcgca tgtggccggg aattgcgctg agctgttcgc tgggaaatat cgccgcatcc
    13621 attccaggaa ggccagcagg tcaaagcagg cgatttactg gcagaaattg accccagcca
    13681 gttcaaagtt gcattagcac aaacccaggg ccaactggca aaagataaag ccacgcttgc
    13741 taacgcccgc cgtgatctgg cgcgttatca gcaactggca aaaaccaatc tcgtctcccg
    13801 tcaggagctg gatgcccaac aggcgctggt cagtgaaacc gaaggcacca ttaaggctga
    13861 tgaagcaagc gtcgccagcg cgcagttgca actcgactgg agccgcatca ccgcaccagt
    13921 cgatggtcgc gttggtctca agcaagttga tgttggtaac caaatctcca gtggtgatac
    13981 caccggaatt gtggtgatca cccagacgca tcctatcgat ttagtcttta ccctgccgga
    14041 aagcgatatc gctactgtag tgcaggcgca aaaagccgga aaaccgctga tggtagaagc
    14101 ctgggatcgc accaattcga agaaattaag tgaaggcacg ctgttaagcc tcgataacca
    14161 aatcgatgcc acaaccggta cgattaaagt gaaagcacgc tttaataatc aggatgatgc
    14221 gctgtttccc aatcagtttg ttaacgcgcg catgttagtc gacaccgaac aaaacgccgt
    14281 agtgatccca acagccgccc tgcaaatggg caatgaaggc cattttgtct gggtgctgaa
    14341 tagcgaaaac aaggtcagca aacatctggt gacgccgggc attcaggaca gtcagaaagt
    14401 ggtgatccgt gcaggtattt ctgcgggcga tcgcgtggtg acagacggca ttgatcgcct
    14461 gaccgaaggg gcgaaagtgg aagtggtgga agcccagagc gccaccactc cggaagagaa
    14521 agccaccagc cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt gttacccccg
    14581 agcagcacag gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac cacgctgctg
    14641 atggtggcga tcttactcgc cgggattatc ggttatcgcg ccctgcccgt ttcggcgctg
    14701 ccggaagtgg actatccgac cattcaggtg gtcacgctct acccaggtgc cagcccggat
    14761 gtcatgacct ctgccgttac cgcgccgctc gaacgccagt tcgggcagat gtctggcctg
    14821 aaacagatgt cgtcgcaaag ttccggcggt gcgtcagtta tcactttgca gttccagcta
    14881 acattaccgc tcaatgtcgc cgagcaggaa gtgcaggccg cgattaacgc tgcgaccaac
    14941 ttgttgccga gcgatctgcc taacccgccg gtttacagca aagtgaaccc ggcagatccg
    15001 ccgatcatga cgctcgccgt cacctcaacc gccatgccga tgacgcaagt ggaagatatg
    15061 gtggaaaccc gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct ggtgacgctt
    15121 tccggcggtc agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat tgccgccctc
    15181 ggcctgacca gcgaaaccgt gcgcaccgcc attaccggcg ctaacgttaa ctcggcaaaa
    15241 ggtagcctcg acggcccttc ccgtgcggtc acgctttccg cgaacgacca gatgcaatcc
    15301 gccgaagagt atcgccagct aatcatcgcc taccagaacg gcgcgccaat tcgtctgggc
    15361 gatgtcgcaa ctgtagagca aggtgcagaa aacagctggc tcggcgcgtg ggcgaacaaa
    15421 gaacaggcca ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat ctccaccgcc
    15481 gacagcattc ggcagatgct gccacagctc actgagagtc tgccgaaatc ggtgaaggtg
    15541 acagtgcttt ccgatcgcac caccaatatc cgcgcatccg tcgatgatac tcagtttgaa
    15601 ttgatgatgg ctatcgcgct ggtagtcatg attatctacc tgtttttgcg caatattccg
    15661 gcgaccatca ttcccggtgt tgctgtaccg ctgtcgttaa tcggcacttt cgcggttatg
    15721 gtgtttctcg atttttcaat caataacctg acactgatgg ctttaactat cgccaccgga
    15781 ttcgtggtcg atgacgccat cgtggtgatc gaaaacattt cccgctatat cgaaaaaggc
    15841 gaaaaaccgt tggcggcggc gctcaagggc gcaggtgaaa tcggctttac cattatctcg
    15901 ctgaccttct cactgattgc ggtgttgatc ccactgctgt ttatgggcga tatcgtcggg
    15961 cgactgttcc gcgaatttgc tattaccctg gcggtagcga ttttgatctc agcggtggtg
    16021 tcgctgaccc tgacaccgat gatgtgcgcg cggatgctca gccaggagtc gttgcgtaaa
    16081 cagaacagct tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc cgcctatggt
    16141 cgtggactgg cgaaagtgct gaatcatccg tggctgacct taagcgtggc actcagcacg
    16201 ctgctgctta gcgtgctgct gtgggtgttc attccgaaag gtttcttccc ggtacaggac
    16261 aacggcatta ttcagggcac tttgcaggca ccgcaatcca gctcctttgc caatatggcc
    16321 cagcgacaac gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca aagcctgacc
    16381 tcatttgttg gcgttgatgg cactaacccg tcgctgaata gtgcacgttt acaaatcaac
    16441 ctcaaaccgt tggatgaacg tgatgatcgg gtgcaaaaag tcatcgcccg tctgcaaacg
    16501 gcggtagata aagtgccggg cgtcgatctc ttcctgcaac caacgcagga cctgaccatc
    16561 gatactcagg tcagccgcac ccagtaccag tttaccttgc aggccacgtc actggatgcg
    16621 ctcagtacct gggtgccaca gttgatggaa aaactccagc aactgccaca gctttctgat
    16681 gtctccagcg actggcagga caaagggctg gtggcgtatg tcaatgttga tcgcgacagc
    16741 gccagccgtc tggggatcag catggcggac gtcgataacg ccctgtacaa cgcgtttggt
    16801 cagcgactga tttccactat ttatactcag gctaaccagt atcgcgtggt gctggagcac
    16861 aataccgaaa ataccccagg cctcgcggcg ctggatacca ttcgcctgac cagcagcgac
    16921 ggtggcgtgg tgccgttaag ctcaattgcc aaaattgagc agcgttttgc gccgctttcc
    16981 atcaaccatc tggatcagtt cccggtaaca accatctcct ttaacgtgcc ggataactat
    17041 tcgctgggcg atgcggtgca ggcgattatg gacaccgaaa agacgctgaa tctgccggtg
    17101 gatatcacca cgcaattcta gggcagcacc ctcgccttcc agtcggcgct gggcagcact
    17161 gtctggctga ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat tctgtacgag
    17221 agctttattc acccgatcac cattctctcg acgctaccca ccgcaggggt cggcgcgctg
    17281 ctggcattaa tgattgccgg tagcgaactg gatgtgattg cgattatcgg cattattttg
    17341 ctgatcggta ttgtgaagaa gaacgccatc atgatgatcg acttcgcgct ggctgctgag
    17401 cgcgagcaag gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt gcgttttcgt
    17461 ccgatcctga tgaccaccct ggcggctctg cttggcgcgc tgccgctgat gttgagtacc
    17521 ggggtcggcg cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg tctgattgtc
    17581 agccaggtgc tgacgctgtt caccacgccg gtgatttatc tgctgttcga ccgcctggca
    17641 ttgtggacca aaagtcgctt tgctcgccat gaagaggagg cgtaagtgaa gttttttgcc
    17701 ctattcattt accgcccggt ggcgacgatt ttactgtcgg tcgccattac cctgtgcggc
    17761 atactgggtt tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt tccggtgatt
    17821 atggtcagcg cctcgctgcc cggtgcgtcg ccggagacaa tggcgtcttc cgttgccacg
    17881 ccgctggagc gctcacttgg gcgcattgcc ggggttagtg aaatgacctc cagcagttcg
    17941 ctcggcagca cgcgcattat tttgcagttt gattttgacc gggatatcaa cggcgcagcg
    18001 cgtgatgtgc aggcggcgat caacgctgca caaagtttgc tgcccagcgg gatgcccagc
    18061 cgcccgacct atcgcaaagc gaacccgtcg gatgcgccaa ttatgatcct cacgctgaca
    18121 tccgatactt attcgcaggg tgaactgtac gatttcgcct cgacgcagct ggctccgacg
    18181 atttcgcaaa tcgacggtgt cggtgatgtc gatgtcggtg gcagctcgct gcccgccgtg
    18241 cgcgtcgggc tgaccccgca ggcgctgttt aatcagggcg tgtcgctgga cgacgtacgc
    18301 actgccatca gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga tggcactcac
    18361 cgctggcaga tccagaccaa cgatgaactg aaaaccgccg ctgaatatca gccgctgatt
    18421 attcactaca acaacggcgg cgcggttcgt ctgggcgatg tggcgacggt gactgactca
    18481 gtgcaggatg tgcgcaacgc cgggatgacc aacgccaaac cggctatttt actgatgatc
    18541 cgcaaactgc cggaagccaa tattatccag acggtagaca gcatccgggc aaaattacca
    18601 gagttgcagg agaccattcc ggcggcgatt gatctgcaaa ttgcccagga tcgctccccc
    18661 accattcgcg cctcgctgga agaagtcgag caaacgctga ttatctcggt ggcgctggtg
    18721 attctggtgg tctttttatt cctgcgctcg ggtcgcgcca ctattattcc cgccgttgcg
    18781 gtgccggttt cgctgattgg tacgtttgcg gcgatgtacc tgtgcggatt cagtctcaat
    18841 aatctttcgc tgatggcgct caccattgct actggtttcg tggtggatga cgccattgtg
    18901 gtgctggaaa acattgcccg tcatctggaa gcggggatga aaccactaca ggccgcgctg
    18961 caaggtactc gcgaagtcgg ctttacggtg ctgtcgatga gtctgtcact ggtggcggtg
    19021 ttcctgccgc tgctgttgat gggcggattg ccgggccgac tgttacgcga gtttgccgtg
    19081 acgctttctg tcgccattgg tatttcgttg ctggtttccc tgacgctaac gccaatgatg
    19141 tgcggctgga tgctaaaagc cagcaagccg cgtgagcaaa agcgactgcg tggttttggc
    19201 cgcatgttgg tagccctgca acaaggctac ggcaagtcgc tgaaatgggt gctcaatcat
    19261 acccgtctgg tgggcgtggt gctgcttggc accattgcgc tgaatatctc gatcccgaaa
    19321 accttcttcc cggagcagga caccggcgta ctgatgggtg ggatacaggc ggatcagagt
    19381 atttcgtttc aggcgatgcg cggcaaattg caggatttca tgaaaattat ccgtgacgat
    19441 ccggcagtgg ataatgtcac cggctttacc ggcggttcac gagttaacag tgggatgatg
    19501 tttatcaccc tcaagccacg cgacgaacgc agcgaaacgg cgcagcaaat tatcgaccgt
    19561 ctgcgcgtga agctggcgaa agaaccgggg gcgaatctgt tcctgatggc ggtacaagat
    19621 attcgcgttg gtgggcgtca gtcgaacgcc agctaccagt acacgttgtt atccgacgac
    19681 ctggcggcac tgcgcgaatg ggagccgaaa atccgcaaaa aactggcgac gttgccggaa
    19741 ctggcggacg tgaactccga tcagcaggat aacggcgcgg agatgaatct ggtttacgac
    19801 cgcgacacca tggcacggct gggaatcgac gtacaggccg ccaacagtct gttaaataac
    19861 gccttcggtc agcggcaaat ctcgaccatt taccagccga tgaaccagta taaagtggtg
    19921 atggaagtgg atccgcgcta tacccaggac atcagtgcgc tggaaaaaat gttcgttatc
    19981 aataacgaag gcaaagcgat cccgctgtcg tatttcgcta aatggcaacc ggcgaatgcc
    20041 ccactatcgg tgaatcatca gggattatcg gcggcctcga ccatctcgtt caacctgccg
    20101 accggaaaat cgctctcgga cgccagtgcg gcgatcgatc gcgcaatgac ccagcttggt
    20161 gtgccttcga cggtgcgcgg cagttttgcc ggcacggcgc aggttttcca ggagacgatg
    20221 aactcgcagg tgatcctgat tatcgccgcc atcgccacgg tgtatatcgt gctgggtatc
    20281 ctttacgaga gttacgtaca tccgctgacg attctctcca ccctgccctc ggcgggcgtt
    20341 ggagcgctgt tggcgctgga gctgttcaat gccccgttca gcctaatcgc cctgataggg
    20401 atcatgctat taatcggcat cgtgaagaaa aacgccatta tgatggtcga ttttgcgctt
    20461 gaagcccaac ggcacggtaa cctgacgccg caggaagcta ttttccaggc ctgtctgctg
    20521 cgttttcgcc cgattatgat gaccaccctg gcggcgctgt ttggcgcgct gccgctggta
    20581 ttgtcgggcg gtgacggctc ggagctgcgg caacccctgg agatcaccat tgtcggcggt
    20641 ctggtgatga gccagctcct gacgctgtat accacgccgg tggtgtatct ctttttcgac
    20701 cgtctgcggc tgcgtttttc gcgtaaacct aaacaaacgg taaccgagta aatgacagat
    20761 cttcccgaca gcacccgctg gcaattatgg attgtggctt tcggcttctt tatgcagtcg
    20821 ctggacacca ccatcgtaaa caccgccctt ccctcaatgg cgcaaagcct cggggaaagt
    20881 ccgttgcata tgcacatggt cattgtctct tatgtgctga ccgtggcggt gatgctgccc
    20941 gccagcggct ggctggcgga caaagtcggc gtgcgcaata ttttctttac cgccatcgtg
    21001 ctgtttactc tcggttcact gttttgcgcg ctttccggca cgctgaacga actgttgctg
    21061 gcacgcgcgt tacagggcgt tggcggtgcg atgatggtgc cggtcggcag gttgacggtg
    21121 atgaaaatcg taccgcgcga gcaatatatg gcggcgatga cctttgtcac gttacccggt
    21181 caggtcggtc cgctgctcgg tccggcgctc ggcggtctgc tggtagaata cgcgtcgtgg
    21241 cactggatct ttttgatcaa cattccggtg gggattatcg gtgcgatcgc cacattgatg
    21301 ttaatgccga actacaccat gcagacgcgg cgctttgatc tttccggctt tttattgctg
    21361 gcggttggca tggcggtatt aaccctggcg ctggacggca gtaaaggtac aggtttatcg
    21421 ccgctggcga ttgctggcct ggtcgcagtt ggcgtggtgg cactggtgct ttatctgctg
    21481 cacgccagaa ataacaaccg cgccctgttc agtctgaaac tgttccgcac tcgtaccttt
    21541 tcgctgggcc tggcggggag ctttgccgga cgtattggca gtggcatgtt gccctttatg
    21601 acaccggttt tcctgcaaat tggcctcggt ttctcgccgt ttcatgccgg actgatgatg
    21661 atcccgatgg tgcttggcag catgggaatg aagcgaattg tggtacaggt ggtgaatcgc
    21721 tttggttatc gtcgggtact ggtagcgacc acgctgggtc tgtcgctggt caccctgttg
    21781 tttatgacta ccgctctgtt gggctggtac tacgttttgc cgttcgtcct gtttttacaa
    21841 gggatggtca actcgacccg tttctcctcc atgaacaccc tgacgctgaa agatctcccg
    21901 gacaatctgg cgagcagcgg aaacagcctg ctgtcgatga ttatgcaatt gtcgatgagt
    21961 atcggcgtca ccatcgccgg gctgttgctg gggctttttg gttcgcagca tatcagcgtc
    22021 gacagcggca ccacacaaac cgtctttatg tacacctggc ttagcatggc gtttatcatc
    22081 gcccttccgg cgttcatctt tgccagagtg ccgaacgata cgcatcaaaa tgtagctatt
    22141 tcgcggcgaa aaaggagcgc gcaatgaagt tctggcgacc cggtattacc ggcaaactgt
    22201 ttctggcgat tttcgccacc tgcattgtct tgctgatcag tatgcactgg gcggtgcgta
    22261 tcagttttga gcgtggcttt attgattaca tcaaacacgg caatgaacag cgattacagc
    22321 tgttaagtga tgcgcttggc gagcagtatg cgcagcatgg taactggcgc ttcctgcgca
    22381 acaacgatcg ctttgtcttt cagatcctgc gttcatttga acacgataat tcggaagata
    22441 aacccggccc gggtatgccg ccgcacggct ggcgtaccca gttctgggtg gtcgatcaaa
    22501 acaacaaagt actggttggt ccgcgagcgc cgattccacc tgacggtaca cggcgaccca
    22561 ttctggtcaa cggtgcggaa gtgggcgcgg tgattgcctc ccctgttgag cggctgaccc
    22621 gcaatactga tatcaatttc gataaacaac agcggcaaac cagctggctg atcgtcgccc
    22681 tggcaacgtt actcgcggcg ctcgccacct ttctgctggc gcgcggtttg ctggcaccgg
    22741 taaaacgact tgtcgatggc acgcacaaac tggcggcggg cgatttcact acccgcgtaa
    22801 cgcccaccag tgaagatgaa ctgggcaaac tggcgcaaga cttcaaccag ctcgccagca
    22861 cgctggagaa aaaccagcaa atgcggcgcg attttatggc cgatatttct cacgaactgc
    22921 gtacgccatt agcggtgctg cgcggtgaac tggaagccat tcaggatggc gtgcgcaaat
    22981 tcacgccgga gacggtggcg tctttacagg cggaggtcgg tacactgacc aaactggttg
    23041 acgatcttca tcagttgtcg atgtctgatg aaggcgctct cgcctatcaa aaagcaccgg
    23101 tagatttgat cccactgctg gaagtggcgg gcggcgcatt tcgcgaacga ttcgccagcc
    23161 gtggcctgaa actgcaattt tccctgccag acagtatcac cgtatttggc gatcgcgacc
    23221 gtttaatgca gttattcaat aacttactgg aaaacagcct gcgctacact gacagcggcg
    23281 gcagcctgaa aatctctgcc gagcagcacg acaaaacggt gcgcctgacc tttgccgaca
    23341 gtgcgccagg tgtcagtgac gatcagctac aaaaattgtt tgaacgtttt tatcgcaccg
    23401 aaggttcccg caaccgcgcc agcggcggtt ccgggctggg gctggcgatt tgcctgaaca
    23461 ttgttgaagc ccataatggc cgcattattg ccgcccattc gccttttggc ggggtaagca
    23521 ttacagtaga gttaccgctg gaacgagatt tacagagaga agtatgaccg agttaccaat
    23581 cgacgaaaac acaccgcgta ttttgatcgt ggaagatgaa ccgaagctgg ggcagttgct
    23641 cattgattat ctgcgtgctg cgagctatgc gccgacgctt atcagccacg gcgatcaggt
    23701 actggcgtat gtgcgccaga caccaccgga tctgatcctg ttggatctga tgctccctgg
    23761 caccgatggc ctgacattat gccgggaaat tcgtcgtttt tccgatgttc cgatcgtgat
    23821 ggtgacggca aaaatcgaag agatcgaccg cctgctgggg ctggagattg gcgccgatga
    23881 ctacatctgc aagccgtaca gcccacggga agtggtagcg cgtgtcaaaa ccattttgcg
    23941 ccgttgtaaa ccgcaacgcg aacttcagca acaggatgcc gaaagcccgt tgattatcga
    24001 cgaaggtcgt tttcaggctt catggcgcgg taaaatgctt gacctgacgc ctgcggaatt
    24061 tcgtctgctt aaaacgctct ctcacgaacc aggaaaagtg ttctcccgcg agcaattgct
    24121 caatcatctt tatgacgact accgcgtagt aaccgaccgc accattgaca gccacattaa
    24181 aaatctgcgc cgcaagctgg aatctctcga cgccgaacag tcatttatcc gcgccgttta
    24241 cggcgttggt taccgctggg aagccgacgc ctgccgcatc gtttagtttt agcgacatta
    24301 ctttgttagc cggagatggc gttcagaccc gatccggcaa cattattcca cgcatggtca
    24361 gcaaactgac attatctctc cgccctggca ggcatcagaa aatcgctcat actttaatca
    24421 gtaaacagca cctttagatg ctgttttcga taagcaattt caatcaagga gtcattatgg
    24481 ctggttggtt tgaactcagt aagagcagtg ataatcagtt ccggtttgtg ctaaaagcgg
    24541 gcaatggtga gactatcctc accagcgagc tgtatacctc aaaagcctct gcggaaaagg
    24601 gcatcgcgtc ggtgcgtagc aacagcccgc aagaagagcg ctatgagaaa aaaacggcaa
    24661 gcaacggcaa attctatttc aatctgaaag ccgctaatca tcaaattatc ggctccagcc
    24721 agatgtacgc caccgcgcaa tctcgtgaaa ccggaattgc ctccgttaaa gccaatggca
    24781 caagccagac ggtgaaagac aatacgtaat tacgatgccg ggcgtgggtt gttggcgtcc
    24841 ggcgctttac cagatccccc ctacagcgct acaatgcccg cccttaaagt gggggcctcc
    24901 cctaaccgct tcatcaggtg aagcggatct gacctgtcat cagaacgaga gaattatgtt
    24961 taaaccggaa ctcctttccc cggcgggaac gctgaaaaat atgcgttacg ctttcgctta
    25021 tggcgcagat gctgtttatg cgggccagcc gcgttactcc ctacgtgtgc gcaacaacga
    25081 attcaaccac gaaaatcttc agctcggcat caatgaagcc cacgcgctgg ggaaaaagtt
    25141 ttatgtcgtg gtcaacattg caccgcacaa cgccaagctg aaaaccttta tccgtgacct
    25201 gaaaccggtg gtggaaatgg ggccggatgc gctgattatg tccgatccag ggctgattat
    25261 gctggtgcgt gagcacttcc ctgaaatgcc gatccacctc tcggtgcagg ctaacgccgt
    25321 gaactgggcg acggtgaaat tctggcagca aatgggactg acccgcgtga tcctctctcg
    25381 cgagctgtcg ctggaagaga ttgaagagat ccataatcag gtgccggata tggagatcga
    25441 gatcttcgtt cacggcgcac tgtgcatggc ctactccggt cgctgcctgc tctctggcta
    25501 tatcaacaag cgcgacccga accagggcac ctgcaccaac gcctgccgct gggagtacaa
    25561 cgtccaggaa gggaaagaag atgacgttgg caacatcgta cacaagtacg agccgattcc
    25621 ggtgcaaaat gttgagccga cgctgggtat cggcgcgcca accgacaaag tgtttatgat
    25681 cgaagaagcc cagcgtccgg gcgagtatat gaccgcgttt gaagatgagc acggcactta
    25741 catcatgaac tcgaaagatc tgcgcgccat cgcccatgta gaacgcctga ccaaaatggg
    25801 cgtgcattcg ctgaaaatcg aaggtcgtac caaatctttc tactattgcg cacgcaccgc
    25861 acaggtttac cgcaaagcta tcgatgacgc cgctgcggga aaaccgttcg ataccagcct
    25921 gctggaaact ctggaaggtc tggcgcatcg tggctatacc gaaggtttcc tgcgtcgtca
    25981 tactcacgac gattatcaga actacgaata cggttattca gtttctgacc gccagcagtt
    26041 tgttggtgag tttaccggtg agcgcaaggg ggacctcgca gcggtagcgg tgaaaaataa
    26101 attctccgtt ggcgacagtc ttgagctgat gacgccgcaa ggcaacatta actttaccct
    26161 tgagcacatg gaaaacgcca aaggcgaagc tatgccggtc gcaccaggcg atggttatac
    26221 tgtgtggctc ccggtcccgc aggatcttga gctaaattac gcgctgctaa tgcgtaattt
    26281 ctccggggaa accacgcgta acccccacgg taagtgatta atttcgatta tttttcccgg
    26341 atggaaaatt cttagaaacc gatcacatac agctgcattt attaaggtta tcatccgttt
    26401 cgctgaaaaa cataatccat aaaatgctag ctgtaccagg aaccacctcc ttagcctgtg
    26461 taatctccct tacacgggct tattttttgt acataagctg taaagaagga ttgatccctg
    26521 ataaccgtct tccattgacc acatcgatag aatccccctt catagcacaa tgcctttcac
    26581 gtaacggtat cgtgctcgcc caggttccgg ctacgcacag ccagaacgcg catgtttgac
    26641 gcttacaaaa aaatattctc actctccaca tttgaatgtc agacgagcga cgccatgtaa
    26701 tcctgcacct tctgtcttca ggtcaactat ctgcattttt ttgccctgag taacacagaa
    26761 atgggctgca tcatttttta ctatattttc tgcaccagat attctgcccc tggctaaaga
    26821 agcttcggct tcggtgtagt attggttatc gagtttacgc tgaatattac ttttatatgc
    26881 aagaccaaat ttaccgatac ttgtttcatc attatgcaca gcacaaccag atataagaaa
    26941 aatactaatt aatgatatag cagctatctt tttcatctca ccttccccca ttaaatacca
    27001 acgacactct ctagtgttta aatataataa tggcatgatt attataattg aataggatta
    27061 taataaatgt tctgtacaac atttcctaca taagtaggaa ttacggacat tgaggccctt
    27121 cagggtaact ccatgggggc tttaatatat tatattgaag atgccactgt ttagttgaat
    27181 attaggtata tgctcttttt tgaaatttat cggtggcagc cgttagtatt cgctgtcccc
    27241 attgcaagct cctggtggta accactgaat cctccatact tgaactgact ttttatcctc
    27301 cgactttcat cctgttctga ctccaccttt tgttttctgc tctacactat ctacagacca
    27361 atcataaagg cacatacgat catggcagaa tttcccgcca gcttactgat tcttaatggt
    27421 aaaagtactg acaatctacc cttgcgcgaa gcaattatgc tgttgcgtga ggaaggaatg
    27481 acgatccatg tgcgggtcac ctgggagaaa ggtgatgccg cacgatttgt agaggaggcc
    27541 cggaagttgg gcgtcgcaac ggtgattgcc ggtggtggcg atggcaccat taatgaagtt
    27601 tctacggcgt tgattcagtg tgagggggat gacatacccg cgctgggaat tttgccatta
    27661 ggaaccgcca atgattttgc caccagtgta gggattcctg aggcactgga taaggcgctg
    27721 aaactggcaa ttgccggtaa tgccattgcg atagatatgg cgcaggtcaa caaacaaacc
    27781 tgttttatta atatggcgac aggcggattt gggacgcgta ttaccacaga aacgccggaa
    27841 aaattaaaag ccgcgctggg tggcgtctct tacatcattc atggcttaat gcgcatggat
    27901 actctgcaac cggaccgttg tgaaatccgc ggtgaaaact ttcactggca aggtgacgcc
    27961 ctggtcattg gtattggtaa cgggcgtcag gccggtggcg gtcaacaatt gtgcccgaac
    28021 gcgttaatta acgatggctt gctgcaactg cgcattttta ccggcgatga aattcttccg
    28081 gctctcgtat caaccttaaa atctgacgaa gataacccga atattatcga aggcgcttcg
    28141 tcgtggtttg atatacaagc cccacacgaa atcactttta atcttgatgg cgaaccgttg
    28201 agtgggcaaa acttccatat tgaaatactt ccggcggcgt tgcgttgtcg attaccacca
    28261 gattgtccat tattgcgtta atcaaactat tctctcttga accagggaga atccgtaatg
    28321 ccggatgcgg tgtaaacacc ttatccggca tacagaacaa tactacaccg taattaattt
    28381 tacccgtgtc ttctgcaatg ccaatttatc cacctctgac acagcatcat cggtaacaat
    28441 aatgtcaatt ttttcgattg gcagtacctg attaaaaccg cgacgattaa atttcgatga
    28501 atcgagaacg gcaatcactt tatttgcggc ggtgaccatc gccccgctaa tcgaataacc
    28561 ttcattaaag gtcgtaatac cattaaccgc atcaatacca tcagcaccga caaacattaa
    28621 atcggcatta atatcctgca atgaacgctc ggcaatggaa ccatgcatcg agcgcgtttt
    28681 atggcggacc gtgccaccac agacgaccag agtaatgtct ttattttcgg aaagggcaaa
    28741 cgctgccggg agactgttgg taatcaccgt gatattttta gcggtcatta atccttcggc
    28801 gataagcatt gtggtacttc cgctgtcgag aataacggtc atcccttcat ggatcatttt
    28861 gactgccgcc tgcgctattc tgttttttgg cgcgctggcg agctgaaatc gctctttaaa
    28921 cccgacttcc tgggtctcgg tttcactatt accggacatt attttcgccg caccgccatg
    28981 aaagcgcgta accacgcctt tttgttcgag aaagcgcaaa tcggcacgga ttgtcgcttc
    29041 cgaggcagca aatactcccg ccagatcctg aacaagcacg gttccctgtt cattcactaa
    29101 ttggatgatc ttattccttc gctcgaatga attcatcgtc atttacccat attcaattgt
    29161 ggctagtgta aacgaagggg gaccattggt gaatgatctg ttttgcacaa taccgggcca
    29221 gcatcacgct ggcccgcggt ttcatggaat gagcaacact ttgccctgca tggcattacg
    29281 agcgatgtca cgcaccgcct gggtgaagct ttcaaagctt ccacggtgag cgattaatgg
    29341 ctccaggctt aacttacgtt ctgtcagcaa ccggctcgcc gtttcccact cctgccccgg
    29401 ccaagggctg gaatagttca tccaactgcc gataaccgtc agctctttac gcaatatttt
    29461 gccaaacgtt gccgatgtta aatgcagatc ctgatgcaac gtgcccacca gcgccagttg
    29521 ggcatgagga ccggcaatct ctaccgccag ttcgacagtt tgcggtacgc cagccgtctc
    29581 gaggataagc tgattaaagc gcagttcgcg taaaacgctc tgcatttgcg gcgcgctcat
    29641 ttcgctactg ttaaatgttt gcatcgcacc gaaagatttt gccagtgcca gtttttctga
    29701 gctaatgtcg atcgccgtca cactctttgc tcccagcgcg acagcgcact gaatagccag
    29761 caggccaatg gttccggcac caataataat aacgttttta ttctcacaac cttgcgctaa
    29821 atgaaaagca tgcaggccaa cggtaatcgg ctcaataaaa gccccatcct caataggcat
    29881 atccgtgggt agagcaaaga cattttttcg cttaacgaca atatattcag caaatccacc
    29941 atcacgccgc gagccaataa aatcatattt tgcgcactgg gaataaaacc ctttcaaaca
    30001 ctctggacaa gtaaaacagg gtaataacgg cacacaggca accgcatcgc cagggtgtaa
    30061 atcatcaaca ccggatccca ccgcatcaat atagccgcta aattcatggc ctaacgttat
    30121 tggataataa tgtgcaccat ttttaaatat cctgggtaaa tcggaaccac ataagcccga
    30181 gctggcaatt tttacccgca cctcatcctg atgtttaatt tcaggaatga cgctttttgc
    30241 aacgcgcacg ataccatcag tatcattcac tactgatttc ataaaaactc ctgat
//
LOCUS       DAJTRD010000041        30062 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTRD010000041 DAJTRD010000000
VERSION     DAJTRD010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30062
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.041"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..68
                     /locus_tag="OO315_002061"
     CDS             <1..68
                     /locus_tag="OO315_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5890978.1"
                     /translation="RFAIREGGRTVGAGVVAKVLS"
     gene            298..681
                     /gene="secE"
                     /locus_tag="OO315_002062"
     CDS             298..681
                     /gene="secE"
                     /locus_tag="OO315_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5890979.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            683..1228
                     /gene="nusG"
                     /locus_tag="OO315_002063"
     CDS             683..1228
                     /gene="nusG"
                     /locus_tag="OO315_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5890980.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1387..1815
                     /gene="rplK"
                     /locus_tag="OO315_002064"
     CDS             1387..1815
                     /gene="rplK"
                     /locus_tag="OO315_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5890981.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1819..2523
                     /gene="rplA"
                     /locus_tag="OO315_002065"
     CDS             1819..2523
                     /gene="rplA"
                     /locus_tag="OO315_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5890982.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2936..3433
                     /gene="rplJ"
                     /locus_tag="OO315_002066"
     CDS             2936..3433
                     /gene="rplJ"
                     /locus_tag="OO315_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5890983.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3500..3865
                     /gene="rplL"
                     /locus_tag="OO315_002067"
     CDS             3500..3865
                     /gene="rplL"
                     /locus_tag="OO315_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5890984.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4185..8213
                     /gene="rpoB"
                     /locus_tag="OO315_002068"
     CDS             4185..8213
                     /gene="rpoB"
                     /locus_tag="OO315_002068"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5890985.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8290..12513
                     /gene="rpoC"
                     /locus_tag="OO315_002069"
     CDS             8290..12513
                     /gene="rpoC"
                     /locus_tag="OO315_002069"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5890986.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12755..13888)
                     /gene="thiH"
                     /locus_tag="OO315_002070"
     CDS             complement(12755..13888)
                     /gene="thiH"
                     /locus_tag="OO315_002070"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5890987.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13885..14655)
                     /gene="thiG"
                     /locus_tag="OO315_002071"
     CDS             complement(13885..14655)
                     /gene="thiG"
                     /locus_tag="OO315_002071"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5890988.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14657..14857)
                     /gene="thiS"
                     /locus_tag="OO315_002072"
     CDS             complement(14657..14857)
                     /gene="thiS"
                     /locus_tag="OO315_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5890989.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14841..15596)
                     /gene="thiF"
                     /locus_tag="OO315_002073"
     CDS             complement(14841..15596)
                     /gene="thiF"
                     /locus_tag="OO315_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5890990.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15589..16224)
                     /gene="thiE"
                     /locus_tag="OO315_002074"
     CDS             complement(15589..16224)
                     /gene="thiE"
                     /locus_tag="OO315_002074"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5890991.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16224..18119)
                     /gene="thiC"
                     /locus_tag="OO315_002075"
     CDS             complement(16224..18119)
                     /gene="thiC"
                     /locus_tag="OO315_002075"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5890992.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18200..18300)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18353..18829)
                     /gene="rsd"
                     /locus_tag="OO315_002076"
     CDS             complement(18353..18829)
                     /gene="rsd"
                     /locus_tag="OO315_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5890993.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18924..19697
                     /gene="nudC"
                     /locus_tag="OO315_002077"
     CDS             18924..19697
                     /gene="nudC"
                     /locus_tag="OO315_002077"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5890994.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19737..20801
                     /gene="hemE"
                     /locus_tag="OO315_002078"
     CDS             19737..20801
                     /gene="hemE"
                     /locus_tag="OO315_002078"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5890995.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20958..21629
                     /gene="nfi"
                     /locus_tag="OO315_002079"
     CDS             20958..21629
                     /gene="nfi"
                     /locus_tag="OO315_002079"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5890996.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21672..22262
                     /locus_tag="OO315_002080"
     CDS             21672..22262
                     /locus_tag="OO315_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5890997.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22449..22721
                     /gene="hupA"
                     /locus_tag="OO315_002081"
     CDS             22449..22721
                     /gene="hupA"
                     /locus_tag="OO315_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5890998.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22734..23429
                     /locus_tag="OO315_002082"
     CDS             22734..23429
                     /locus_tag="OO315_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5890999.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23431..23856)
                     /gene="zraP"
                     /locus_tag="OO315_002083"
     CDS             complement(23431..23856)
                     /gene="zraP"
                     /locus_tag="OO315_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5891000.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24094..25470
                     /gene="zraS"
                     /locus_tag="OO315_002084"
     CDS             24094..25470
                     /gene="zraS"
                     /locus_tag="OO315_002084"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5891001.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25467..26792
                     /gene="zraR"
                     /locus_tag="OO315_002085"
     CDS             25467..26792
                     /gene="zraR"
                     /locus_tag="OO315_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5891002.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26789..28078)
                     /gene="purD"
                     /locus_tag="OO315_002086"
     CDS             complement(26789..28078)
                     /gene="purD"
                     /locus_tag="OO315_002086"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5891003.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28090..29679)
                     /gene="purH"
                     /locus_tag="OO315_002087"
     CDS             complement(28090..29679)
                     /gene="purH"
                     /locus_tag="OO315_002087"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5891004.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7034 a         7837 c         8274 g         6917 t
ORIGIN      
        1 tgcgtttcgc aatccgtgaa ggcggccgta ccgttggcgc gggcgttgta gcaaaagttc
       61 tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca tttgatgccc
      121 tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt cataatcatt
      181 gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt ttcgatttgg
      241 tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg ttggtttatg
      301 agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa gtgggtcgtt
      361 gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga cattatgctg
      421 ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt cgcgctgtta
      481 acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga agtccgtaag
      541 gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc tgcggttacc
      601 gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct ggtatccttt
      661 atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt acgtcgttca
      721 ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata tcaaattaca
      781 caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg ttgaaatccg
      841 tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc tcgttcagat
      901 ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga tgggcttcat
      961 cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg cgattatgaa
     1021 ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac cgggtgaaat
     1081 ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag aagtggatta
     1141 cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc cggtagagct
     1201 ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg atttaatcgt
     1261 tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg gccttgcccg
     1321 taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca acttgaggaa
     1381 tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc tggtatggct
     1441 aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat catggaattc
     1501 tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat tccggtagta
     1561 atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc ggcagcagtt
     1621 ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa agacaaagtg
     1681 ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc cgacatgact
     1741 ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc catgggcctg
     1801 gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat ccgtgagaaa
     1861 gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa agagctggcg
     1921 actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga cgctcgtaaa
     1981 tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg ttccgttcgc
     2041 gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg cgcagaactg
     2101 gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt tgacgttgtt
     2161 attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct gggtccgcgt
     2221 ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga agcggttaaa
     2281 aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat ccacaccacc
     2341 atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc tctgctggtt
     2401 gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa agttagcatc
     2461 tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc ttctgtaaac
     2521 taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta taatgcttaa
     2581 tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt gagtcgccaa
     2641 gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc acaagttgaa
     2701 tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc tccgtcgaag
     2761 accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga acgctaagat
     2821 tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct ctccgtttgg
     2881 aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa agctaatggc
     2941 tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag ccaaaggcgc
     3001 gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga ctgaactgcg
     3061 taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc tgctgcgccg
     3121 tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc cgaccctgat
     3181 tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt tcgcgaaagc
     3241 gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc cggcgtctca
     3301 gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc tgatggcaac
     3361 catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac gcgatgcgaa
     3421 agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa acttattctg
     3481 atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag cagttgcagc
     3541 tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat tcggtgtttc
     3601 cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag aaaaaactga
     3661 attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca aagcagtacg
     3721 tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg caccggctgc
     3781 tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc tggaagaagc
     3841 tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga gaaatcaggc
     3901 tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc agtagcgttt
     3961 cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga cttaatatac
     4021 tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt gatagcaaca
     4081 ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca aactgtccgc
     4141 tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt tactcctata
     4201 ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg gatgtacctt
     4261 atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat cctgaagggc
     4321 agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac agcggtaatt
     4381 ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc caggaatgtc
     4441 aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg gtgatctatg
     4501 agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc tacatgggcg
     4561 aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag cgtgttatcg
     4621 tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt aaaacccact
     4681 cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc tggctggact
     4741 tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt aaactgcctg
     4801 cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac ctgttctttg
     4861 aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg ccggaacgcc
     4921 tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac gtagaaaaag
     4981 gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc aaactgatcg
     5041 aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt gatgagtcta
     5101 ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg gctaagctga
     5161 gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat cacggcccat
     5221 atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca ctggtagaaa
     5281 tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa agcctgttcg
     5341 agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt atgaagttca
     5401 accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa gacgacatca
     5461 ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc gatgatatcg
     5521 accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac cagttccgcg
     5581 ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc gatctggata
     5641 ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg aaagagttct
     5701 tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct gagattacgc
     5761 acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt gcaggcttcg
     5821 aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa acccctgaag
     5881 gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac gaatacggct
     5941 tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa attcactacc
     6001 tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac ctggatgaag
     6061 aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc agcttgttca
     6121 gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc gtcggtgcgt
     6181 ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt gcgaatatgc
     6241 aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact ggtatggaac
     6301 gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt gtcgttcagt
     6361 acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat ccgggtgaag
     6421 caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac acctgtatca
     6481 accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg ctggcagacg
     6541 gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta gcgttcatgc
     6601 cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt gttcaggaag
     6661 accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac accaagctgg
     6721 gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc tccaaactgg
     6781 atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt ctggttggta
     6841 aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg cgtgcgatct
     6901 tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac ggtgtatccg
     6961 gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac aaacgtgcgc
     7021 tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa gaactgcaga
     7081 tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt ggcgttgaag
     7141 ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg acagacgaag
     7201 agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa cacgagttcg
     7261 agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg gcaccgggcg
     7321 tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct ggtgacaaga
     7381 tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc gaagatatgc
     7441 cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc gtaccgtctc
     7501 gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa ggtatcggcg
     7561 acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc gaattcatcc
     7621 agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt accttcagcg
     7681 atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc gcaacgccgg
     7741 tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc gacctgccga
     7801 cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag cgtccggtaa
     7861 ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag atgcacgcgc
     7921 gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag gcacagttcg
     7981 gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc gcagcataca
     8041 ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc aagatgtata
     8101 aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc ttcaacgtat
     8161 tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag taattctcgc
     8221 tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa ctccgacggg
     8281 agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac cgaagagttt
     8341 gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc tttcggtgaa
     8401 gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga cggccttttc
     8461 tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa gtacaagcgc
     8521 ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca gactaaagta
     8581 cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat ctggttcctg
     8641 aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga tatcgaacgc
     8701 gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct ggaacgtcag
     8761 cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga cgaattcgac
     8821 gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct ggagcaagag
     8881 tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg taaaaagctg
     8941 accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc agagtggatg
     9001 atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc gctggatggt
     9061 ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa ccgtaacaac
     9121 cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa cgaaaaacgt
     9181 atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg tgcgatcacc
     9241 ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa acagggtcgt
     9301 ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt aatcaccgta
     9361 ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct ggagctgttc
     9421 aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat taaagctgcg
     9481 aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga agttatccgc
     9541 gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat ccaggcattt
     9601 gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg tgcggcatat
     9661 aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct ggaagcccag
     9721 ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc gaacggcgaa
     9781 ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac ccgtgactgt
     9841 gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga acgtctgtat
     9901 cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga gtatgaaaaa
     9961 gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt tggccgtgcc
    10021 attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca ggcgctgggt
    10081 aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct gaaaccgacc
    10141 gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg ttctggtgca
    10201 tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat ctccgaggca
    10261 gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac tgcgggcgaa
    10321 cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc caaagcgatg
    10381 atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga gaagcaggtt
    10441 tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc ggcacagatt
    10501 cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat catcgaaacg
    10561 ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat ctccacccac
    10621 ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg ttacctgact
    10681 cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg tggtacccat
    10741 gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc gctgcgcgat
    10801 cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc tgatattctc
    10861 gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga gaactctgtc
    10921 gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt atgtgcgcac
    10981 tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc aatcggtgtt
    11041 atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac gttccacatc
    11101 ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa caaaggtagc
    11161 atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt tatcacttcc
    11221 cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag ctacaaagtg
    11281 ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg cgaaaccgtt
    11341 gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt tgtacgcttt
    11401 actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac cggtctgtct
    11461 tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct gcgtccggca
    11521 ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga tatgcctgcg
    11581 cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca gatcagctct
    11641 ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat caccggtggt
    11701 ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc aatcctggct
    11761 gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg tctggttatc
    11821 accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg tcagctcaac
    11881 gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc ggaagcgccg
    11941 cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt taacgaagta
    12001 caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga agttatcgtt
    12061 cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt cctggaaggc
    12121 gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc gaacggcaaa
    12181 gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct ggcaaccgag
    12241 tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga agcagccgtt
    12301 gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg tcgtctgatc
    12361 ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc tgcgggtgaa
    12421 gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc agaactgctg
    12481 aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata acgtaaaaac
    12541 ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca cggaaaatat
    12601 taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat tcctgctgac
    12661 ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat cttccgtagc
    12721 ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag gcgcgtccca
    12781 gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac acggcagcaa
    12841 ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg tgattatcgg
    12901 catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc agcggaataa
    12961 cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc tccggtgcaa
    13021 gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc gacgcaggct
    13081 caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg ctttgccagt
    13141 aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg cgccagttgt
    13201 cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc cccagccgat
    13261 ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga tggcgggtat
    13321 aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca agttgcttta
    13381 actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa gagaactgtt
    13441 cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc tgatgttcac
    13501 cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt tccctggcaa
    13561 tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat ccgcagtacg
    13621 tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta actgtgttgc
    13681 caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga tagccactgg
    13741 cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca tttagcgccc
    13801 gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc cagtccagtt
    13861 gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg tcagcgggct
    13921 ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca gcaggcctac
    13981 ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga cggcaatcgc
    14041 cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat ggctgggaac
    14101 gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa tctccagcat
    14161 ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca tcaccgctgc
    14221 acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt aaggcagcac
    14281 gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg gatcgggcaa
    14341 cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta acgcttcacg
    14401 tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag tattcggcag
    14461 cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct ggcgcaagtc
    14521 gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg cctccaccat
    14581 cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa acgttttgtc
    14641 cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc tggtcgccat
    14701 cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta atcgccagag
    14761 ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt tgcccggcgg
    14821 cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca taccgggcaa
    14881 ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc gaacagtcgg
    14941 agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag tgcctgcaaa
    15001 gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt gcgttctggc
    15061 tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg tgtcagtacc
    15121 atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt gagtgccacg
    15181 caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag caccacatcg
    15241 gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa tgctgtcagt
    15301 tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt cgggctgttg
    15361 atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac atcgtcgtca
    15421 tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc aggtgtaccc
    15481 agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg cccgtcaaga
    15541 gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc attcatcgcc
    15601 aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg cttgagtaat
    15661 ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg cgcgtgccag
    15721 actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat gccgtgccaa
    15781 ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga acacatgtcc
    15841 cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat cgtcatgtgt
    15901 cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg cttgcaaatc
    15961 ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc agtaatcgtt
    16021 gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga catcggcttc
    16081 cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc ctgcatccaa
    16141 cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac gaaaaggtac
    16201 aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat ttctccgcct
    16261 ctggcacgga agttctccga catatccgcc attcccactt caatggtttg cgcggcggcg
    16321 taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca catggagcaa
    16381 aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc gcgggcggta
    16441 aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg ggctttcgac
    16501 atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc agcgtgggcg
    16561 gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag acccagatgc
    16621 tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat cgccgcacca
    16681 atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg ccccagagtg
    16741 taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg gatcatctgc
    16801 atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca ggcgattttg
    16861 gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc gtcctgaata
    16921 gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc acagatttca
    16981 caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag gcaccatttc
    17041 gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt catcggcaca
    17101 tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc ggcctgttcc
    17161 agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt aaccttctcc
    17221 agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa aatccactcg
    17281 cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc tccccagcgc
    17341 gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc cgagttgccg
    17401 atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc cgattccgga
    17461 tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg gacaaattcc
    17521 gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg gtggcgtaaa
    17581 acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa ttccatttcc
    17641 ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc tgttttggcg
    17701 cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc tgccagccgc
    17761 gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg cgcatcgatc
    17821 cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg cggatcacca
    17881 tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg cggctgttct
    17941 ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac gcgcacgccg
    18001 gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc ttccagggtg
    18061 tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt tgcagacata
    18121 gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca agagacgatc
    18181 gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta ttagcctgat
    18241 caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg acaagaaatt
    18301 agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga actcaagcag
    18361 gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc agcacaaagc
    18421 gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg taattatcat
    18481 gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg gtattggctt
    18541 cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc agcttatgaa
    18601 gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg ctctgacaaa
    18661 aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc ttaatgccaa
    18721 ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga tcaaccagtt
    18781 tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg attgactccg
    18841 caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa acatgccagt
    18901 gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat cacggctggt
    18961 gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca tatggcgaag
    19021 cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg cagggggaac
    19081 ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt caggtcattg
    19141 atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg gagttttacc
    19201 gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa accgaatggg
    19261 cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc tgcattattg
    19321 ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat cgtaacggtg
    19381 tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag gcagtcgcgc
    19441 gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg acttctcagc
    19501 cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac agcggcgaca
    19561 tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac gatttgccgt
    19621 tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg gcgatgtgtc
    19681 gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca gccaaaatga
    19741 ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt gatgtcactc
    19801 cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc acgcgcgccc
    19861 aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa gtgactttgc
    19921 aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc ctcaccgtgc
    19981 cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt tttacctcgc
    20041 cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa gatgagctgg
    20101 gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa gtgccgctga
    20161 ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc ggcagcagca
    20221 aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg cacgctctgc
    20281 tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa gccggtgctc
    20341 aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat tatcaacagt
    20401 tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac gacggtcgcc
    20461 gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc atggctgaaa
    20521 ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg cgccgccgtg
    20581 tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac gcgccgcctg
    20641 cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc gaaggtcatg
    20701 tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct ggcgtgttcg
    20761 tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat ccatcaggct
    20821 attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact acgtgtacgc
    20881 tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc tgttgaacca
    20941 ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc gaactggctt
    21001 cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc gccggagccg
    21061 atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg ctgaaatatc
    21121 cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg ccttacattc
    21181 caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag atgctgtcgc
    21241 aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc cgtcttggcg
    21301 tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg aaaaaacggc
    21361 tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca ctgatggata
    21421 aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg ttgtttatcg
    21481 ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc tgcatgaaag
    21541 gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa cgtccggcgt
    21601 tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat ttccgtattt
    21661 gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg gaaagctggc
    21721 agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac gccatgttct
    21781 gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat ctcatctggg
    21841 aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag aaatttgaag
    21901 aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca atcgatgcct
    21961 gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc gaacacgcgg
    22021 tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact caggctggtc
    22081 gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa tgggacattc
    22141 agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag ctgataaaag
    22201 gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt tttcagcaat
    22261 aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc cctttcgccc
    22321 cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg tatgcaggag
    22381 agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac acattgtaag
    22441 gataacttat gaacaagact caactgattg atgtaattgc agagaaagca gaactgtcca
    22501 aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag tctctgaaag
    22561 aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat cgcgctgagc
    22621 gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct aacgtaccgg
    22681 catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg gcagtgaaca
    22741 gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca ttgatgctgg
    22801 caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc tttaccgcca
    22861 gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc ggcgataatg
    22921 tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg cgagagtatc
    22981 gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa ccgccggtga
    23041 acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt gaatccgatg
    23101 gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt gctgatcaga
    23161 tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag gggcgctggc
    23221 atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct gatttagatt
    23281 cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat gtcagcgtgg
    23341 catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct gatttctgtc
    23401 gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca tatgaccgcc
    23461 accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac ccgcttcagc
    23521 catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat tctccatctc
    23581 tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca gggcattgta
    23641 ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt aaaagtcatt
    23701 atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat tttgctgcca
    23761 cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg ctgaaagcgc
    23821 catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag tggttgtctt
    23881 acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg gtaatctggc
    23941 atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt tactcattga
    24001 aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc atgatttctg
    24061 ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg ttctaaagac
    24121 tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct ggtagggttg
    24181 tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag acaaacgctg
    24241 ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt cggcatgggg
    24301 atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg gcagcctgga
    24361 gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag caactccggc
    24421 atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc gggagatgaa
    24481 gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat ttatcgccag
    24541 tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca gtatgccgcg
    24601 acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc cgaagatcgt
    24661 gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct ggcaagcgta
    24721 ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca agatgaaatg
    24781 aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc ccacgaaatc
    24841 cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg cgcgcctgca
    24901 gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg tttaaaccgc
    24961 gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca ggcggtggat
    25021 ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa cagccgggag
    25081 atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga tccggacagg
    25141 ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg tcagcatggc
    25201 gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt taccgacagc
    25261 ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt caccaccaaa
    25321 gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca acacggtggt
    25381 acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg gcttccggtc
    25441 aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt ctggtggtgg
    25501 atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg ggctataacg
    25561 tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag gtttttgatc
    25621 ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg aaagagatca
    25681 aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc gtcgagacgg
    25741 cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg gatttcgata
    25801 acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat gctgaaacac
    25861 ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg caacacctgc
    25921 tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac ggcgattccg
    25981 gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt agcgaaaaac
    26041 cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct gaattgttcg
    26101 gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc tttgttgagg
    26161 cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg atgcaggtgc
    26221 gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac cagactatct
    26281 cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag gtgaatgccg
    26341 ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa gtaccatcgc
    26401 tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag cgctttgccg
    26461 agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg ctgatccact
    26521 acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg gtggtgctgc
    26581 tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt acgccgatcc
    26641 cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag gtgattctgg
    26701 cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta gggatcacgc
    26761 gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg gcgcgccaac
    26821 cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag gcgtaggcgc
    26881 gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg cgcccgccgc
    26941 tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact ttgccgtcag
    27001 ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg cccggatatc
    27061 cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac gttttctcgt
    27121 ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc atgcgcagca
    27181 taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc ttcggattgc
    27241 cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg ccttccgccg
    27301 ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga acttcatcgg
    27361 ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg ttatctttat
    27421 cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg ccgtccacca
    27481 tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg cgatgacccg
    27541 cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct tcttccagcg
    27601 tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg acgattggcg
    27661 cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc tggtattccg
    27721 ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag ccttccagtt
    27781 gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag gtatcgacca
    27841 cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt tcgttttgtg
    27901 cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc agcgcgggtt
    27961 ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc ggcgactggg
    28021 ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat actttcatct
    28081 attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa taccgtgctc
    28141 gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga ttacgcaggt
    28201 cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg cgtcagaagc
    28261 catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg cgattttcgc
    28321 ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt ttttggcata
    28381 gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg catcacgcag
    28441 ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac cgaccatccc
    28501 caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa gacccggaac
    28561 acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg cggtgatttt
    28621 cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac gagaaatgat
    28681 ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc cgaatgcgga
    28741 ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc cgatagctac
    28801 accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact ctttcacgca
    28861 ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac cctgaacctg
    28921 ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg cagcctgctg
    28981 gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt tcagcgtgcg
    29041 tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca tgctgccgaa
    29101 gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga tggcgaggtc
    29161 gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa taatggcgtc
    29221 gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg cggagcgcac
    29281 catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc agccttcacg
    29341 agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag gctggatctg
    29401 atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac catgtacttt
    29461 cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt aatcggaaac
    29521 ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg tagacagcag
    29581 ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt tgtcagaaac
    29641 actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc tgggtttgac
    29701 tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc cctaacgggg
    29761 catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg cgaatttttc
    29821 tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac agaaattaca
    29881 ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt gctggggaat
    29941 gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa ctccctataa
    30001 tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg ttcggttgaa
    30061 ga
//
LOCUS       DAJTRD010000042        29788 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTRD010000042 DAJTRD010000000
VERSION     DAJTRD010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29788
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.042"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            57..851
                     /locus_tag="OO315_002088"
     CDS             57..851
                     /locus_tag="OO315_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5891005.1"
                     /translation="MKIFKYTPENNLFYGYILEDMKTGFNILREIMVEGYRPSIARLY
                     DAEDGTQHFTHFADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETW
                     FNNLNWGPDKVAAERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDI
                     TMLGGHSSHSYQNGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVH
                     HHGIGKHRVHWSKLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            873..2282
                     /locus_tag="OO315_002089"
     CDS             873..2282
                     /locus_tag="OO315_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5891006.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            2260..3039
                     /locus_tag="OO315_002090"
     CDS             2260..3039
                     /locus_tag="OO315_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5891007.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            3036..3896
                     /locus_tag="OO315_002091"
     CDS             3036..3896
                     /locus_tag="OO315_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5891008.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(4044..4619)
                     /locus_tag="OO315_002092"
                     /pseudo
     CDS             complement(4044..4619)
                     /locus_tag="OO315_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4636..4896)
                     /locus_tag="OO315_002093"
     CDS             complement(4636..4896)
                     /locus_tag="OO315_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5891009.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4887..6158)
                     /locus_tag="OO315_002094"
     CDS             complement(4887..6158)
                     /locus_tag="OO315_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5891010.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(6236..6598)
                     /gene="queD"
                     /locus_tag="OO315_002095"
     CDS             complement(6236..6598)
                     /gene="queD"
                     /locus_tag="OO315_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5891011.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6917..8716
                     /gene="cysJ"
                     /locus_tag="OO315_002096"
     CDS             6917..8716
                     /gene="cysJ"
                     /locus_tag="OO315_002096"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5891012.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8716..10428
                     /gene="cysI"
                     /locus_tag="OO315_002097"
     CDS             8716..10428
                     /gene="cysI"
                     /locus_tag="OO315_002097"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5891013.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            10502..11236
                     /gene="cysH"
                     /locus_tag="OO315_002098"
     CDS             10502..11236
                     /gene="cysH"
                     /locus_tag="OO315_002098"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5891014.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            11500..11652
                     /locus_tag="OO315_002099"
     CDS             11500..11652
                     /locus_tag="OO315_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5891015.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11846..12937
                     /locus_tag="OO315_002100"
                     /pseudo
     CDS             11846..12937
                     /locus_tag="OO315_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(13083..14120)
                     /gene="iap"
                     /locus_tag="OO315_002101"
     CDS             complement(13083..14120)
                     /gene="iap"
                     /locus_tag="OO315_002101"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5891016.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            14372..15280
                     /gene="cysD"
                     /locus_tag="OO315_002102"
     CDS             14372..15280
                     /gene="cysD"
                     /locus_tag="OO315_002102"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5891017.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            15282..16709
                     /gene="cysN"
                     /locus_tag="OO315_002103"
     CDS             15282..16709
                     /gene="cysN"
                     /locus_tag="OO315_002103"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5891018.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16709..17314
                     /gene="cysC"
                     /locus_tag="OO315_002104"
     CDS             16709..17314
                     /gene="cysC"
                     /locus_tag="OO315_002104"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5891019.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            17364..17687
                     /locus_tag="OO315_002105"
     CDS             17364..17687
                     /locus_tag="OO315_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5891020.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17881..18192
                     /gene="ftsB"
                     /locus_tag="OO315_002106"
     CDS             17881..18192
                     /gene="ftsB"
                     /locus_tag="OO315_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5891021.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            18211..18921
                     /gene="ispD"
                     /locus_tag="OO315_002107"
     CDS             18211..18921
                     /gene="ispD"
                     /locus_tag="OO315_002107"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5891022.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18921..19400
                     /gene="ispF"
                     /locus_tag="OO315_002108"
     CDS             18921..19400
                     /gene="ispF"
                     /locus_tag="OO315_002108"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5891023.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            19397..20446
                     /gene="truD"
                     /locus_tag="OO315_002109"
     CDS             19397..20446
                     /gene="truD"
                     /locus_tag="OO315_002109"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5891024.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            20427..21188
                     /gene="surE"
                     /locus_tag="OO315_002110"
     CDS             20427..21188
                     /gene="surE"
                     /locus_tag="OO315_002110"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5891025.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            21182..21808
                     /gene="pcm"
                     /locus_tag="OO315_002111"
     CDS             21182..21808
                     /gene="pcm"
                     /locus_tag="OO315_002111"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5891026.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21948..23087
                     /gene="nlpD"
                     /locus_tag="OO315_002112"
     CDS             21948..23087
                     /gene="nlpD"
                     /locus_tag="OO315_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5891027.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            23150..24142
                     /gene="rpoS"
                     /locus_tag="OO315_002113"
     CDS             23150..24142
                     /gene="rpoS"
                     /locus_tag="OO315_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5891028.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(24262..24669)
                     /locus_tag="OO315_002114"
     CDS             complement(24262..24669)
                     /locus_tag="OO315_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5891029.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24816..25409
                     /locus_tag="OO315_002115"
     CDS             24816..25409
                     /locus_tag="OO315_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5891030.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            25409..26356
                     /locus_tag="OO315_002116"
                     /pseudo
     CDS             25409..26356
                     /locus_tag="OO315_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26629..27192)
                     /locus_tag="OO315_002117"
     CDS             complement(26629..27192)
                     /locus_tag="OO315_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891031.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            27357..27608
                     /locus_tag="OO315_002118"
     CDS             27357..27608
                     /locus_tag="OO315_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5891032.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            27610..29706
                     /locus_tag="OO315_002119"
     CDS             27610..29706
                     /locus_tag="OO315_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5891033.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAAFAVSAEYHKQQQRIKGMRK
                     RAEEKIRDAEDEGIQILEHKQAEPWLSNVYRPVGNVVAVQQPEYEEEHDEEFERDFRL
                     GMQKLFAMQEEDDPLA"
BASE COUNT         7290 a         7385 c         8060 g         7053 t
ORIGIN      
        1 ttcgtcacat catcatcggc aacgaaggtg cattgtgcta tatcactgaa gtaacagtga
       61 aaatctttaa atacaccccg gaaaacaacc tcttctacgg ctatatcctg gaagacatga
      121 aaaccggctt caacatcctg cgtgaaatca tggtggaagg gtatcgtccg tcgatcgctc
      181 gtttgtatga cgctgaagat ggcacccaac acttcaccca ttttgccgac ggaaaatgtg
      241 tgctgatctt tatggctgaa ggtaaccctc gcattgcgaa ggcgacgggc gaagggattg
      301 cggaaatcgt tgcccgctac ccgcagtgcc agcgcgtgga cagcaaactg attgaaacct
      361 ggtttaacaa cctgaactgg ggaccggata aagtggctgc cgaacgtgtg cagatcctca
      421 aaaccggcaa catgggcttt accaccgaag tgtccggctg ctggagctgc atccacgaaa
      481 tctacgaaag cgttattaac cgtattcgta ctgagttccc gcacgccgac gacatcacca
      541 tgctgggcgg tcactcctct catagctatc agaacggcac caacatgtac ttcgtctatg
      601 actacaacgt tgttgactgt aagccggaag aggaaatcga caagtaccac aatccgctca
      661 acaaaatcat ctgcgaagaa accattcgcc tcggcggttc gatggtgcac caccatggca
      721 ttggtaaaca tcgcgttcac tggagcaagc tggaacacgg cagcgcgtgg gcgttgctgg
      781 aagggctgaa aaagcagttc gatcctaacg gcattatgaa tacgggtact atctatccga
      841 ttgaaaaata atgtatcagg cagcgttccg cgatgacggg gcgttgcctt ttcggcttct
      901 caggcgagaa gccgttctta ttaccggaca atgaaggggt aaagatgaac acttcaccgg
      961 tgcgaatgga tgatttaccg cttaaccgtt ttcactgtcg aattgctacg ctcactttcg
     1021 gcgcacacct gaccgacggt tatgttctag gcgtcattgg ttacgccatt attcaactta
     1081 cgcccgccat gcaactgacg ccgtttatgg cgggaatgat cggcggctcg gcgctccttg
     1141 gtttgtttct tggcagcctg gttcttgggt ggatctccga ccatattggt cggcaaaaaa
     1201 tcttcacctt cagctttttg ctgattacgc tcgcttcgtt cttgcaattt tttgccacca
     1261 cgccagagca tcttattggg ctgcgcattt tgatcggcat tggtctggga ggcgattact
     1321 cagtaggtca caccttgctg gctgaatttt ccccgcgccg ccatcgcggt attttgctgg
     1381 gcgcattcag cgtggtgtgg accgtaggct atgtgttggc aagtattgcc ggacatcact
     1441 ttatttccga aaacccggag gcctggcgct ggttgctggc atcggcagct ctgcccgcgt
     1501 tgttgattac gttattacgc tggggaacgc cagaatcgcc acgttggcta ctgtgccagg
     1561 ggcgttttgc agaagctcac gctatcgtgc atcgctattt tggtccccat gttttactgg
     1621 gcgatgaagt ggtaacggcg acccataaac acatcaaaac cttgttctct tcgcgttact
     1681 ggcggcgcac ggcgtttaac agcgtcttct ttgtctgcct cgtaatccca tggtttgtga
     1741 tttatacctg gctgccaact atcgcccaga ctattggtct ggaagatgcg ctgactgcca
     1801 gcctgatgct taatgcgttg ttaattgtgg gcgcgctgct gggattagtt ctgacgcacc
     1861 tgctggcaca tcgcaaattt ttgctgggaa gttttttgct gctggcggca acgctggtag
     1921 taatggcctg tttgccttcc ggcagttcat taacgctgct gctttttgtt ctcttcagca
     1981 ccaccatttc ggcagtcagt aatctggtgg gcattttgcc tgcggaaagt tttcctactg
     2041 acattcgctc gctgggagtc ggttttgcca ccgccatgag tcgacttggc gcggcggtaa
     2101 gtactggcct gctgccgtgg gtgctggcgc agtggggaat gcaagtcacc ttattgctcc
     2161 tagcgacagt gttgttggtt ggttttgttg tgacctggct atgggcacca gaaactaaag
     2221 ccctcccgct ggtggcggcg ggaaatgtag gaggtgcgaa tgaacattct gttagcgttt
     2281 aaagccgaac cggatgccgg aatgctggcg gaaaaagagt ggcaggcggc ggctcagggt
     2341 aatagcggac cggatgtttc gctactgcga agtttactcg gtgctgatga acaggccgct
     2401 gccgcgctgc tgttagcgca gagaaaaaac ggtacgccga tgtctttaac cgcgttgagc
     2461 atgggggatg aacgggcgtt gcactggctg cgctatctca tggctcttgg gtttgaggaa
     2521 gctgtattgc tggagacggc ggcagatctg cgctttgcgc cggaatttgt cgctcgccat
     2581 attgccgaat ggcagcgtca gaatccgctg gatctgataa ttaccggcag ccaaagcagc
     2641 gaagggcaga atgggcaaac gccatttttg ctggcggaaa tgctgggctg gccctgcttc
     2701 acccaggtgg agcgtttcac tctcgacgcg ctgtttatca ccctcgaaca gcgtactgaa
     2761 catgggttgc gctgttgccg ggttcgcttg cctgcggtga ttgccgtgcg tcagtgcggt
     2821 gaagtggcct tgccagtacc gggaatgcgc cagcgaatgg ctgcggggaa agcagaaatt
     2881 atccgtaaaa ctgttgctgc cgaaatgcca gcgatgcagt gtctgcaact ggcaagagca
     2941 gagcaacgac gcggagcgac gctgattgat gggcaaacgg tagcagaaaa agctcaaaag
     3001 ctatggcagg actatttacg ccaaaggatg cagccatgaa tatcgcaatt gtgacaataa
     3061 atcaggagaa tgccgcaata gcgagctggc tggcggcgca ggatttcagc ggttgtacgc
     3121 tggcgcactg gcaaatagaa cctcagcccg tagtggcgga acaggtgctg gatgcgatgg
     3181 tggaacagtg gcagcgaacg cctgcggacg ttgttctctt tccgccggga acgtttggcg
     3241 atgaactgtc gacccggctg gcctggcgtt tacatggtgc aagcatctgt caggtgactt
     3301 cgttagatat tccaaccgta agcatgcgca aatcccactg gggaaatgcg ttaacggcga
     3361 cgctgcaaac agaaaaacgc ccgttatgcc tttccctggc gcgtcaggcg ggagcagcga
     3421 aaaacgccac gttgccctct ggaatgcagc aactgaacat tgtcccaggt gcgcttcccg
     3481 actggctggt tagcacagag gatctgaaaa atgttagccg cgacccatta gctgaggcca
     3541 gacgggtgct ggtggtggga cagggcggag aggcagataa ccaggaaatc gttatgctgg
     3601 cagaaaagct gggagccgaa gtgggctaca gtcgggcgcg agtgatgaac ggtggcgttg
     3661 atgctgaaaa agtgattggt atttccggac atctgctggc acctgaggtg tgcattgttg
     3721 ttggcgcttc cggcgcagca gcgttaatgg cgggcgtacg caatagcaag tttatggtgg
     3781 cgattaatca tgacgccagc gcagcggtat tttcacaggc agatattggc gtggttgatg
     3841 actggaaagt ggtgctggag gcactggtga caaacattca cgcagaatac caataacgtg
     3901 ctgccggatg atcctgccta cggttctgga ctggggacct gtaggcctga taagacgcgt
     3961 taagcgtcgc atcaggcgct gaatgtcgga tgtggcgtgc ggccaacaaa tcaaaacaaa
     4021 ttcaattggt tacgattatc ccgtcaaagc aattttttcg ctaacgtcca gactccggta
     4081 ttcgtggtgg aaagcgccac gacacccgcg ttaatcgcat tacgcgcatc ttcttcatcg
     4141 cataccagcc cgccagcaat cagcggttgg cggatttttt ccgtcaccca gcccagcact
     4201 ttgggcatgc agcctggcag gatctcaata caatccggat tcgattgcgc aacttactta
     4261 tcaatgttgt gaaacgaaat cgaatcaaca ataaacaggt gatgaataca gaaaaaaact
     4321 tccgctcttg ccgcttttag catcgatgct ttggtgctga taattccatc cgcctcggtc
     4381 accagtttca aaaactgaat caccacttct ttatttgacg cgccttccag caaatcaaca
     4441 tgaataaaag catatttccc ggcgttctta atctttttaa cgatattact gatggtgcag
     4501 atattgccgt acaacacgga aataaactgg cattcagaat cgatggccag ttgcaggctg
     4561 gcattatctt taacggcagc aatcaccgga ttctggcgga gcaggtgtaa gaggggcata
     4621 aattatcctt attatttatc caaagcggta ggtgatgccg aatccggaag gcggatagcg
     4681 ccactgttgt agtgtggatt cgtcgcacag caaacggcag gtgccacact ccaggcagcc
     4741 gcgatagtca atacgtaagt caccttcctg tgtgagagaa aaaagaccta ccggacaggc
     4801 gttaatcaac cgttgtacca tctgacgctc aacggagtca gccggaacaa tgtgcggcgc
     4861 atcagcaacg cgccagagat tacgggctac agacatcgca gactcctgat aacatcgccc
     4921 gccagatgcc acaggccatg acgacgtaaa tgatgccaga ataactggcg cagcggtgga
     4981 acaggtttat caccctgatc ccataaatcg cgggaaatat cctgcattaa cgcaggccac
     5041 gcccgatacc tgccagggcg ttgcaaaagc gccggaacat gctgataacg ttgtagaaca
     5101 tcccatagca ggctgcgctc tacgttgtga tgataaagcg caaacagatt ttgcggctcg
     5161 cggtgctggc aggcgcttat tagcgtttgt gccgccgcct gtgcgccagt cagcgccata
     5221 tccatgccgc gcacggaaat tccggtattg acgcaactgc gcaacgcatc gcccaccagc
     5281 agccagccgt taccggcgta ttgcaccggc atactgtgca agccaccttc tggcaccaga
     5341 tgcgcaccat actccagtga ttccgtgttt ttgataagcg tgcgcactgc cggatgcgct
     5401 ttaaagcgag tcagcagctc gcttgccgga acacgacttt gcgtaaggga agagagcggg
     5461 caaacaatcc ctaacgagag cgtttgttga ttagtataaa gaaatgcgcc gccgggtaag
     5521 tcatcacaga ttccgccgct gaacagcaac gctgcgcctt cgttattctc cagatgaaaa
     5581 cgttcttcaa tagcggatgt ttccagcgac agcacttctt tgatccccaa cgccatcgct
     5641 tcgccagcag gacgagtcac taacccgtga cgttcagcca ggacgctgtt ggcaccttct
     5701 gccagcacca cataacgggc gcggagaata tcgtcaccac aaataacgcc acagactctg
     5761 ccgttttctt catacagtgc atccaccgtc gctccgggga tgcattcgac accttctttt
     5821 tcggcttcgg caaccagcca cggatcgaac cgtgcacgta atacattcca ggattcaccg
     5881 ccgggctgta agctggaaaa cgtcgttgcg ccatccggcg ttaacaggga aaggctttcg
     5941 tgagtgatgc gtcgttcaag aggcgcggtc agatgaaatt gcgggaggag ttccgcgagt
     6001 gcatgggtat ataaccgcct gccggaaaga tttttgctgc cggggatttc agcgcgttcg
     6061 agtaacaaaa cggataaatc cgctcgcgcg cagcgtaacg cgcaagcggt gcctgcaata
     6121 ccggcaccaa taataataat gtcgcagtcg tcttccatgt catgccccag atatccagaa
     6181 cacaacaccc taacatagcg ttactaaagg gaaattgacc gccgacacac tcttatcatt
     6241 cgccgcgata gatacaccct gcggtgcagg tttcttttac catcaccgca cttaacagcg
     6301 gcacaacggg tttaacctga tcccaaatcc attttgctaa aacttcgctg gttggatttt
     6361 ccagacctgg aatatcattg agataatggt gatcgaggcg ctcataagtt ggcttaaacg
     6421 ccgctttcag ttcagcgaaa tcgataatcc agcccgtatg cggatcgact tccccggtaa
     6481 tttccagtcg caccataaag gaatgcccgt gcaggcgacc acatttatgc ccttccggga
     6541 catgtggtaa gcggtgagcg gcttcgaagg tgaaatcttt aaataacgtg gtggacatca
     6601 taatttctct acaggacgat aaaaccgccg tagagtaccg gaaaggctgt tttttcgcat
     6661 tatctaaaac gactttgttg cgcaaaatcg ctgatttatc ttaatgattg gctaaattca
     6721 tttgtttttc attaggttgg ttaatctatt ttgttgttaa agactattgc taaaacaggt
     6781 tagtcgattt ggttattagt tatcgctatc ccgtctttaa tccacaccgt tcaccccgtt
     6841 aaccttacct tctcttctgt tttatgggcg ctgacagggc gcagaaacag ctttgcttac
     6901 tggaacataa cgacgcatga cgacacaggt cccaccttcc gcgttgcttc cgttgaaccc
     6961 ggagcaactg gcacgccttc aggcggccac gaccgattta acgcctaccc agcttgcctg
     7021 ggtttctggc tatttctggg gcgtgctcaa ccagcagcct gttgcgcttg cagtgacgcc
     7081 agcgcccgcc gcagaaatgc cgggtataac gattatctct gcttcgcaaa ccggcaatgc
     7141 gcgccgggtt gctgaagcat tacgcgatga tttattagca gcaaaactga acgttaagct
     7201 ggtgaacgcg ggcgactata aattcaaaca aatcgccagc gaaaaactgc tcatcgtagt
     7261 gacgtcaacg caaggggaag gggaaccgcc ggaagaagcc gtcgcgctgc ataagttcct
     7321 gttctccaaa aaagcgccaa agctggaaaa caccgcgttt gccgtgttta gcctcggcga
     7381 tagctcttat gaatttttct gccagtccgg gaaagatttt gacagcaagc tggcggaact
     7441 ggggggtgaa cgcctgctcg accgtgtcga ttctgatgtt gaataccagg ctgctgccag
     7501 cgagtggcgc gcccgcgtgg ttgatgcgct taaatcgcgt gcgcctgtcg cggcaccttc
     7561 gcaatccgtc gctactggcg cggtaaatga aatccacacc agcccgtaca gcaaagacgc
     7621 gccgctggtg gctagcctct ctgttaacca gaaaattact gggcgtaact ctgaaaaaga
     7681 cgttcgccat atcgaaattg acttaggcga ctcgggcctg cgttaccagc cgggtgacgc
     7741 gctgggcgtc tggtatcaga acgatccggc actggtgaaa gaacttgtcg aactgctgtg
     7801 gctgacaggc gatgaacctg tcactgtcga gggcaaaacg ttgcctctga acgaagcgct
     7861 acagtggcac ttcgaactga ccgtcaacac cgccaacatt gttgagaatt acgccacgct
     7921 tacccgcagt gaaacactgc tgccgctggt gggcgataaa gcgaagttac agcattacgc
     7981 cgcgacgacg ctgattgttg acatggtgcg tttctccccg gcacagcttg atgccgaagc
     8041 gctaattaat ctgctgcgcc cgctgacccc gcgtctctat tccattgcct cctcgcaggc
     8101 ggaagtcgag aacgaagtac acgtcaccgt tggtgtggtg cgttacgacg tggaaggccg
     8161 cgcccgtgcc ggtggtgcct ccagcttcct cgctgaccgc gtggaagaag agggcgaagt
     8221 ccgcgtattt atcgaacata acgataactt ccgcctgccc gctaacctgg aaaccccggt
     8281 gattatgatt ggcccaggca ccggtattgc gccgttccgc gcctttatgc agcaacgcgc
     8341 cgctgacgaa gcgccaggta aaaactggct gttctttggc aatccgcatt ttacggaaga
     8401 cttcctgtat caggtggagt ggcagcgcta cgtcaaagag ggtgtgctga cgcggatcga
     8461 tctcgcctgg tcgcgcgacc aaaaagaaaa agtttacgta caagataaac tgcgcgaaca
     8521 gggcgcggag ctgtggcgct ggatcaatga cggtgcccac atttatgtct gcggcgacgc
     8581 taatcgcatg gcgaaagacg ttgagcaggc acttctggaa gtgattgccg aatttggtgg
     8641 catggacacc gaagcggcgg atgaattttt aagtgagctg cgcgtagagc gccgttatca
     8701 gcgagatgtc tactaatgag cgaaaaacat ccagggcctt tagtggtcga aggaaaactg
     8761 acagacgccg agcgcatgaa gcttgaaagc aactacctgc gcggcaccat tgcggaagat
     8821 ttaaacgacg gtctgaccgg cggctttaag ggcgataact tcctgctgat ccgcttccac
     8881 ggcatgtatc agcaggatga ccgcgacatc cgcgccgaac gtgctgaaca gaagctggag
     8941 ccgcgccacg cgatgctgtt gcgctgccgt ctgccgggtg gagttatcac caccaaacag
     9001 tggcaggcga tcgataaatt tgccggtgaa aacaccatct atggcagcat tcgcctgacc
     9061 aaccgccaga cgtttcagtt ccacggcatt ctgaaaaaga acgtcaaacc ggtgcaccag
     9121 atgctgcact cggtcggtct tgatgcgctg gcgactgcta acgacatgaa ccgtaacgta
     9181 ctctgcacct cgaaccctta cgagtcgcag ctgcacgcag aagcgtacga gtgggcgaag
     9241 aagatttctg aacatctgct gccgcgtacc cgcgcgtatg cggagatctg gctcgatcag
     9301 gaaaaagtcg ccaccaccga tgaagaaccg atccttggtc agacttacct gccgcgtaag
     9361 ttcaaaacca cggtagtgat cccgccgcag aacgatattg atctgcacgc caacgacatg
     9421 aacttcgtgg cgatcgcgga aaacggcaag ctggtgggct tcaacctgct ggtgggcggt
     9481 gggctttcta tcgaacacgg taacaagaaa acctacgccc gcacggcgag tgagtttggc
     9541 tatctgccgc tagagcatac gctagcggtg gcggaagccg tcgtgacaac tcagcgtgac
     9601 tggggtaacc gaaccgatcg taaaaatgcc aaaaccaaat acacactgga gcgcgtgggg
     9661 gttgagacgt ttaaagcgga agtggagcgt cgcgcgggga tcaaatttga accgatccgc
     9721 ccgtatgaat ttacaggacg cggcgatcgt attggctggg ttaagggcat tgatgataac
     9781 tggcacctga cgctgtttat cgaaaatggt cgcatccttg attatccggg gcgtccgctg
     9841 aaaactggcc tgctggagat cgcgaagatc cacaaaggtg atttccgcat tacggcgaac
     9901 cagaatctga tcatcgccgg tgtgccggaa agcgagaaag cgaagatcga gaaaattgcc
     9961 aaagagagcg ggttaatgaa tgccgtcacg ccgcagcgtg aaaactcgat ggcctgcgtg
    10021 tcattcccga cttgcccgct ggcgatggcg gaagcggagc gtttcctgcc gtcttttatc
    10081 gacaacatcg ataatttaat ggtgaaacat ggtgtcagcg atgagcatat cgtgatgcgt
    10141 gtaacaggct gcccgaacgg ttgtggtcgc gcgatgctgg cggaagtggg cctggtgggt
    10201 aaagcaccgg gtcgctacaa cctgcatctt ggcggcaacc gcattgggac acgtatccca
    10261 cggatgtata aagaaaacat caccgagccg gaaatcctgg cgtcgcttga tgaactgata
    10321 gggcgctggg cgaaagagcg cgaagcgggt gaaggcttcg gcgactttac ggtgcgtgcg
    10381 ggcatcattc gcccggtgct cgatccggcg cgcgatttgt gggattaacc atcagcccgg
    10441 tcttgtaggc ctgataagac gcgtgagcgt cgcatcaggc aaggcaaaca gtgaggaatc
    10501 tatgtccaaa ctcgatctaa acgccctgaa cgaactgccg aaggtagatc gcattctggc
    10561 gctggcagaa actaacgccg aactggaaaa actggacgct gaaggccgcg ttgcctgggc
    10621 gctggataat ctgcccggtg aatatgtact ttcttccagc tttggcattc aggcggcggt
    10681 gagcctgcat ctggtgaatc agattcaccc ggatattccg gtgatcctca ccgacaccgg
    10741 ctatctgttc ccggaaacct accgctttat tgacgagtta acggacaaac tcaagctcaa
    10801 cctgaaagtg taccgcgcta ccgaaagcgc cgcctggcag gaagcacgct acggcaaact
    10861 gtgggaacag ggcgttgaag gcattgaaaa gtacaatgac atcaacaaag tcgaaccgat
    10921 gaaccgggcg ctgaaagaac tgaatgcgca aacgtggttt gctggcctgc ggcgtgaaca
    10981 atccggcagc cgtgccaatt taccggtgct ggcaattcag cgtggcgtat ttaaagtgct
    11041 gccgattatc gactgggata accgaactat ttatcagtac ctgcaaaaac atggcctgaa
    11101 atatcaccca ttatgggacg aaggatattt atcggtgggc gatacccata caacccgtaa
    11161 atgggaaccc ggcatggcgg aagaagaaac gcgtttcttt ggcttaaaaa gggaatgtgg
    11221 attacacgaa gggtaaatat tgccggataa attaaacgcc tctgtcagaa atgatggggg
    11281 cgtttttatt ttgcgagcag cattccggca tttaaaggtg aacatcgcag ggaaaattaa
    11341 caggtataga atggcgttgg gtgcttgagg ctgtctggcg ttaagcattc gcgaggttcc
    11401 agatggacaa aagccccagg cgatatttct atcaacctga ggctcgcgtt cgaacgtagc
    11461 aattcggatg ttagtctctt ctttgcaggg aggcaagaca tgctgacaaa atatgccctt
    11521 gtggcaatca tcgtactgtg ttgtacagta ctgggattca cgttgatggt aggtgactcg
    11581 ttatgtgagt tgagtatcag agaacgtggt atggagttta aggcagttct cgcttacgaa
    11641 tcgaagaagt agccaccacg cgggggtaaa accccgcgta ctgaattgtg atggtttggt
    11701 ctcctgcacc ctttttttaa atttatattt tttatgtgaa tatatagtct ctatttatta
    11761 ttatgaattt caaatgaaaa tcattaattt attcataatg taaatgtttt attggctgat
    11821 atatatcata gatagttttt aaaatatgcg taaatatcct ttaagtttac tgaaggataa
    11881 aaatattgtg actttctttg atttctgggg aaaaaaccga cgtggcgaga aagagggtat
    11941 cgacgactat caccttcttt gctggcattt gctggatgtg gccgcaatgg gctatttaat
    12001 ggttaaaaga aattgcttcg ggctggctga ttattttcgt caattaggga tttctgacaa
    12061 ggaacaggca gctcgatttt tcgcctggtt gctgtgctgg cacgatattg gaaaatttgc
    12121 ccgctctttt caacaacttt acctgccccc tgaattcaag attcaggaag gtgccagaaa
    12181 aaattacgaa aaaatctctc attcaacgct gggctactgg ttgtggaatc attatttaag
    12241 tgaatgtcag gagttacttc cttcatcttc gctctctccg cgcaaactta ggcgagtgat
    12301 agaaatgtgg atgcccgtga cgacagggca tcatggtcgg ccccctgatc gtatggatga
    12361 attggataat tttctgcctg aagacaaagc tgcagcacga gattttctcc ttgaaatcaa
    12421 gccactgttt ccgcgaatag agatccccac attctgggat gatgacgagg gcgttgaact
    12481 tataaaacaa ctttcctggt atatctctgc aaccgtcgta cttgcagact ggacagggtc
    12541 gtcaacgcga ttttttccac gcgtcgcaca ggcaatggat attgaagatt actggcaaaa
    12601 ggctttagtt caggctcaaa acgcattaac cgtctttccg ccacaagcag aaaccgcgcc
    12661 tttcaccgga attaatacgc tgtttccttt tattgagaat acgacaccgt tacagcaaaa
    12721 ggtgctggat ctggatatca atcagccagg accacagttg tttattctgg aagatgtgac
    12781 cggcgcaggt aaaacagaag cggcgcttat cctggcgcac aggttgatgg ctgcgggtaa
    12841 cgctcagggg ctgttttttg gcctgccaac aatggcaacg gccaatgcca tgtacgatcg
    12901 actggtcaaa acctggcttg ctttctattc gccagaggtg ttccccgctc cagcggagat
    12961 aaaccggcgc aatacggtgc ggttttcccg gaagtaaacc ggacgcactg gatgcgatga
    13021 tgggtatcac ttagaattcc ccgcccctgc ggtagaactc ccagctccca ttttcaaacc
    13081 catcaagacg ccttcgccag ctccttcacc aacggtagca ttatccgcat aacgtcacgg
    13141 cagcgacgtt ctattcttcc aggaagtgcc ttatcaatat gttgttgatt atccagtctt
    13201 acgtcatgcc agctatttcc cgccgggaag gcaggtgttt ttgcgcgttg ctgataacca
    13261 tccttatttc caagattcca gttagtcgct tccaccgaaa gtacagcaat gcccgctttg
    13321 tcgaatattt ctgcgtcatt acaaccccca gtgcctttcg gataattttt attcaaaccc
    13381 ggattggtcg ttgcggctat tccgtgactg cgtgcaattg ccagcgccct gtcgcgcgtt
    13441 aatttcctta ctgcttcagg ggtttttaca ccgctgttga aatacaattt atcgccaaca
    13501 attaagttat cgagattaat caccagcagc gtatttttct tttcggtgtc actcatccgc
    13561 ttgagtaaat tctcagcgcc taattttcct tcctcttcgc cgctggtcgc cacaaatcga
    13621 ataccatact cggtaggcgt atttttcagg cgttctgcca gttccagcat gacacctaaa
    13681 cctgcggcgt tatcatctat tccttgtaac gtcagtccgc cgagattggc atctgcgtca
    13741 gcatcgctca gcggggcgta agtatccaga tgcgccataa tgatgatctg ctgcggcgct
    13801 ttgccttcat gagcggcaat caccgtactt cctgtcacgt tatgccagtt cttgcgatta
    13861 tcgcgggcgg tataaatata ccgactatta aatgtccgaa tatcactgcg ataacccatt
    13921 tgctgaaact gttggcgaat ataatcggca gataacattt ctgctggagt tccggtcatg
    13981 cgtcccggaa agaaagtagc aatatgtcgt gcctgggtat tagcaaaatc gccaggttta
    14041 ggtgacgagg cgtgtacggg gagaataaag catacgccga gcgccagggc agcggtacgg
    14101 tggcgcaatg cggaaaacat agtgagtcct taaataccat gcaaattttt ttaccgccat
    14161 agtatgaaac tgccgctgcg ctaaaacaat ttcaaatctt cctaaacgcc cgaaatccgg
    14221 tgccttaagc actttttgat attagctttg ccaaatcgtt attccgttaa ggaactactc
    14281 attctaattg gtaatttcat tcgttctctt acgctcccta tagtcgaaac atctgatggc
    14341 aagaaaatag cggtattgca aaggaacggt tatggctcaa aaacgactta ctcacctgcg
    14401 gcaactggag gcggaaagca tccacattat tcgcgaggtg gcggcagaat tctcaaatcc
    14461 ggtgatgctc tactctatcg gtaaagattc cagcgtcatg ctgcatctgg cgcgcaaggc
    14521 gttttatcca ggtacgctgc ctttcccgtt gctgcatgtc gataccggct ggaaattccg
    14581 cgagatgtat gagttccgcg atcgtacggc gaaagcctac ggctgcgagc tgctggtgca
    14641 taaaaacccg gaaggcgtgg cgatggggat taatccattc gtgcacggca gcgcgaaaca
    14701 taccgatatt atgaaaactg aaggcctgaa acaggcgctg aacaaatacg gttttgatgc
    14761 cgccttcggt ggtgcgcgcc gtgacgaaga gaaatcccgc gctaaagagc gaatctactc
    14821 tttccgtgac cgcttccatc gctgggatcc gaaaaatcag cgcccggagc tgtggcacaa
    14881 ctacaacggg caaattaaca aaggcgaaag catccgcgtc ttcccgctct ctaactggac
    14941 cgagcaggat atctggcaat acatctggct ggaaaatatc gacattgtgc cgctgtatct
    15001 cgctgcggaa cgtccggttc tggaacgcga cggtatgttg atgatgattg atgacaaccg
    15061 tatcgacctg caaccgggcg aagtgatcaa aaaacggatg gtgcgtttcc gtacgctggg
    15121 ctgctggccg ctgaccggtg cggtggagtc aaatgcacaa acactgccgg aaatcatcga
    15181 agagatgctg gtttccacca ccagtgaacg tcagggtcgc gtgattgacc gcgaccaggc
    15241 gggatccatg gagctgaaaa aacgtcaggg gtatttttaa gatgaacacc gtacttgcac
    15301 aacaaatcgc caatgaaggc ggcgtcgaag cctggatgat tgcgcaacaa cataaaagcc
    15361 tgctgcgttt tctgacctgt ggtagcgtcg atgacggcaa aagtaccctg attggtcgtc
    15421 tgctgcacga tacccgccaa atctacgaag atcagctctc atcgctgcat aacgacagta
    15481 agcgtcacgg cacccagggc gaaaagctgg atctggctct gctggtggac ggcctgcaag
    15541 ctgagcgcga acagggcatc actattgatg tggcctaccg ctatttctct accgagaagc
    15601 gtaaatttat tatcgccgac accccagggc acgagcagta cactcgcaat atggcgactg
    15661 gcgcatcgac atgtgaactg gcgatcttac tgattgatgc ccgtaaaggc gtgctcgatc
    15721 aaacccgtcg tcacagtttt atctccacac tgttggggat caaacatctg gtcgtggcga
    15781 tcaacaaaat ggatctggtg gattacagtg aagagacgtt cacccgtatt cgtgaagatt
    15841 atctgacctt tgccgggcag ctgccgggta atctggatat ccgctttgtg ccgctctccg
    15901 cactggaagg tgacaacgtg gcttcgcaaa gtgaaagtat gccgtggtac agcggtctga
    15961 cactgctcga agtgctggaa accgtggaga tccagcgagt ggtggatgcc cagccaatgc
    16021 gcttcccggt gcagtacgtt aaccgcccaa atctcgattt tcgtggctac gccggaatgc
    16081 tggcatccgg tcgcgtggaa gtcgggcaac gtgtaaaagt gctgccctct ggtgtggaat
    16141 caaacgtcgc gcggatcgtg acttttgatg gtgatcgcga agaagtcttt gccggagaag
    16201 cgatcaccct ggtgctgacg gatgagatcg atatcagccg tggtgatctg ctgctggcgg
    16261 cagacgaagc gttaccggcg gtgcagagtg cgtcggtgga tgtggtatgg atggcggaac
    16321 agccgctttc cccgggccag agttatgaca tcaaaattgc cggtaagaag acgcgtgctc
    16381 gtgttgatgg cattcgttat caggttgata ttaataacct tacccaacgc gaagttgaaa
    16441 acctgccgct gaacggcatc ggcctggtgg atctcacttt tgacgagcca ctggtgttag
    16501 atcgctatca gcaaaatccg gttaccggtg ggctgatttt tatcgatcgc ctgagcaatg
    16561 tgaccgtggg tgccggtatg gtgcacgagc cagttagcca ggcaacagct gcgtcatctg
    16621 aattcagtgc attcgaactg gaattgaatg ccctggttcg tcgccacttt ccgcactggg
    16681 gcgcgcgcga tttgctgggg gataaataat ggcgctgcat gacgaaaacg tcgtctggca
    16741 tagtcatccg gtcactgtgc aacaacgcga actacaccac ggtcatcgtg gtgtagtgct
    16801 gtggtttacc ggcctctccg ggtccggtaa atcaacggtc gccggggcgc tggaggaggc
    16861 gttacataaa ctcagcgtca gtacgtatct gctggatggc gacaatgttc gccacggatt
    16921 atgcagcgat ctcggtttta gcgatgccga tcgtaaagag aatatccgtc gcgtcggtga
    16981 agtggcgaat ttgatggttg aagccggact ggtggtgctg accgcattta tctcgccaca
    17041 ccgcgccgaa cgccagatgg ttcgcgaacg cgtaggagaa gggcgcttta ttgaagtgtt
    17101 tgtcgatacg ccgctggcga tttgcgaagc ccgcgatcca aaaggcttat ataagaaagc
    17161 gcgtgccggt gaactgcgca actttacggg aatagattcc gtttacgaag cgcctgaatc
    17221 ggcagaaatt catctcaatg gtgaacaatt agtaacaaat ttggtacagc aattattaga
    17281 tctgttgaga cagaacgata ttatcagatc ctgagacacc accgggtttc catgcccggt
    17341 tcgtcaaagt aacaggatca aatatgcgta atagccataa cattacacta acaaataacg
    17401 acagccttac tgaggatgaa gaaactacat ggtcactgcc tggtgccgtg gtcggtttta
    17461 tctcctggtt atttgcgctg gcgatgccga tgttgattta tggctctaac acgctgttct
    17521 tctttctcta cacctggcct ttctttctgg cactgatgcc tgtcgcggta gtggtgggga
    17581 ttgcgctgca ttcattgatg gacggaaagc tacgctacag tattgttttc accctggtga
    17641 cagtaggcat tatgtttggc gcactgttta tgtggctact gggctaattt gtactttctt
    17701 gtctcctctg ttcataattc aaaccgtaac taataatgag attatgttct gcacgccctg
    17761 ggtatacgta acaatgggca aatgtggtac atttgcccgc gttgtcgcgg tatccccaac
    17821 agaggatgta gagtcgtctt cggatgcatg ggatgatgat gcagtttttc agggggcagg
    17881 atgggtaaac taacgctgct gttgctggct attctggtct ggctacagta ttcgctgtgg
    17941 ttcggtaaga acggtataca tgactatacc cgcgtcaatg atgatgtggc ggcactgcaa
    18001 gctacaaacg cgaaacttaa agcgcgaaac gatcaacttt ttgccgaaat tgacgatctc
    18061 aatggcggcc aggaggcgct cgaagagcgt gcgcgtaatg aactcagcat gaccaggccg
    18121 ggcgaaactt tttatcgtct ggtgcctgac gcgtcgaagc gcgcacagtc tgcggggcaa
    18181 aacaatcgat aaatcagccc gggaattaac atggcaacca ctcatttgga tgtttgcgcc
    18241 gtggttccgg cggccggatt tggccgtcga atgcaaacgg aatgtcctaa gcaatatctc
    18301 tcaatcggta atcaaaccat tcttgaacac tcggtgcatg cgctgctggc gcatccccgg
    18361 gtgacacgtg tcgtcattgc cataagtcct ggcgatagcc gttttgcaca acttcctctg
    18421 gcgaatcatc cgcaaatcac cgttgtagat ggcggtgatg agcgtgccga ttccgtgctg
    18481 gcaggtctga aagccgctgg cgatgtgcag tgggtattgg tgcatgacgc cgctcgtcct
    18541 tgtttgcatc aggatgacct tgcgcgattg ttggcgttga gcgaaaccag ccgcacggga
    18601 gggatcctcg ccgcaccagt gcgcgatact atgaaacgtg ccgaaccggg caaaaatgcc
    18661 attgctcata ccgttgatcg caacggctta tggcacgcgc tgacgccgca atttttccct
    18721 cgtgagctgt tacatgactg tctgacgcgc gctctaaatg aaggcgctac cattaccgac
    18781 gaagcctcgg cgctggaata ttgcggattt catccgcagt tggtcgaagg ccgcgcggat
    18841 aacattaaag tcacgcgccc ggaagatttg gcactggccg agttttacct cacccgaacc
    18901 atccatcagg agaatacata atgcgaattg gacacggttt tgacgtacac gcctttggcg
    18961 gtgaaggccc aattatcatt ggtggcgtac gcattcctta cgaaaaagga ttgctggcgc
    19021 attctgatgg cgacgtggcg cttcatgcgt tgaccgatgc attgcttggc gcggcggcgc
    19081 tgggggatat cggcaaactg ttcccggata ccgatccggc atttaaaggt gccgacagcc
    19141 gcgagctgct acgcgaagcc tggcgtcgta ttcaggcgaa gggttatacc cttggcaacg
    19201 tcgatgtcac tatcatcgct caggcaccga agatgttgcc gcacattcca caaatgcgcg
    19261 tgtttattgc cgaagatctc ggctgccata tggatgatgt taacgtgaaa gccactacta
    19321 cggaaaaact tggatttacc ggacgtgggg aagggattgc ctgtgaagcg gtggcgctac
    19381 tcattaaggc aacaaaatga ttgagtttga taatctcact tacctccacg gtaaaccgca
    19441 aggcaccggg ctgctgaaag ccaatccgga agactttgtg gtggtggaag atttgggctt
    19501 tgagcctgat ggtgaaggtg agcatattct ggttagaatc ctcaaaaacg gctgcaatac
    19561 ccgttttgtg gcggatgcac tggcgaaatt cctgaaaatt cattcccgtg aagtcagctt
    19621 cgctgggcaa aaagacaaac atgctgtaac ggaacagtgg ttatgtgctc gcgtgccggg
    19681 caaggaaatg cccgatctga gcgcctttca actggaaggc tgccaggtgc tggagtatgc
    19741 gcggcacaag cgcaagctgc gtttaggggc gctgaaaggt aacgccttta ccctggttct
    19801 gcgcgaagtg agcaatcgcg atgacgttga acaacgtctg atcgatattt gcgtaaaagg
    19861 tgtaccgaac tacttcggtg cccaacgttt tgggattggc ggtagcaact tgcagggcgc
    19921 gctgcgctgg gcgcaaacca atactccggt gcgcgatcgc aataaacgga gtttttggtt
    19981 gtcggcagcc cgcagtgcgt tgtttaatca gattgttgct gagcgcctca aaaaagcaga
    20041 cgttaatcaa gttgttgacg gcgatgcgct acaattagcc ggacgtggta gctggtttgt
    20101 agcaaccacc gaagaactgg cggaattaca gcgtcgcgtc aacgataaag agctgatgat
    20161 aaccgccgca ttgccgggca gtggcgaatg gggaactcag cgtgaagcgc tggcattcga
    20221 acaagcagct gtcgccgcag aaactgaatt acaagcttta ctggtgcgcg aaaaagttga
    20281 agccgcgcgc agagcgatgc tgctgtatcc gcaacaatta agctggaact ggtgggatga
    20341 cgtcaccgta gagatccgtt tctggcttcc ggcgggtagt tttgcaacca gcgttgtcag
    20401 ggaacttatc aacacaacag gtgattatgc gcatattgct gagtaattat gacggggtac
    20461 atgcacccgg tatacaaacg ctggcgaaag ccttgcgtga gtttgctgac gttcaggtgg
    20521 tcgcccccga tcgtaaccgc agcggcgctt caaattctct gacactggaa tcctccctgc
    20581 gcacgtttac ctttgaaaat ggtgatattg ctgtgcaaat gggaaccccg accgattgtg
    20641 tctatcttgg cgtgaatgct ctgatgcgtc cgcgcccgga cattgttgtg tccggaatta
    20701 acgccgggcc gaatctgggg gatgatgtta tttattccgg tacggtagcc gccgcgatgg
    20761 aaggccgtca tttaggtttt ccggcgctgg ccgtctcgct tgacgggcat aaacattacg
    20821 acactgccgc ggcggtaacc tgttcaattt tgcgcgcact gtgtaaagag ccgctgcgca
    20881 ccggacgtat tcttaatatt aacgttcagg atttaccctt ggatcaaatc aaaggtattc
    20941 gcgtgacgcg ctgcggtaca cgacatccgg cagatcaggt gatcccgcag caagatccgc
    21001 gcggcaatac gctgtactgg attggcccgc cgggcggtaa atgtgatgcc ggtccgggga
    21061 ccgattttgc tgcggtagat gagggctatg tctccatcac gccgctgcat gtggatttaa
    21121 ctgcgcatag cgcgcaagat gtggtttcag actggttaaa cagcgtggga gttggcacgc
    21181 aatggtaagc agacgcgtac aagcacttct ggatcaatta cgtgcgcaag gtattcagga
    21241 tgagcaggtg ctgaatgcac ttgccgccgt gccgcgtgaa aaattcgttg atgaagcgtt
    21301 tgaacaaaaa gcctgggaca atatcgcctt gccgataggt caggggcaga caatttcgca
    21361 gccgtatatg gtggcgcgaa tgaccgaatt actcgagctg acgccgcagt cgcgggtgct
    21421 ggaaattggc actggttcgg gatatcaaac ggcaatcctg gcgcatcttg tccagcatgt
    21481 ttgctcggtt gaacggatta aaggcttgca gtggcaggca cgtcgccgcc tgaaaaatct
    21541 tgatttacat aatgtttcaa cccgtcatgg tgatggatgg caaggttggc aggcacgtgc
    21601 gccgtttgac gctatcattg ttacggcggc accgccggaa attccaactg cgctaatgac
    21661 gcagctggac gaaggcggga ttctcgtctt acccgtaggg gaggagcacc agtatttgaa
    21721 acgggtgcgt cgtcggggag gcgaatttat tatcgatacc gtggaggccg tgcgctttgt
    21781 ccctttagtg aagggtgagc tggcttaaaa cgtgaggaaa tacctggatt tttcctggtt
    21841 attttgccgt aggtcagcgt atcgtgaaca tcttttccag tgttcagtag ggtgccttgc
    21901 acggtaatta tgtcactggt tattaaccaa tttttcctgg gggataaatg agcgcgggaa
    21961 gcccaaaatt caccgttcgc cgcattgcgg ctttgtcact ggtttcgcta tggctggcag
    22021 gctgttctga cacttcaaat ccaccggcac cggtcagctc cgttaatggc aatgcgcctg
    22081 caaatactaa ttctggtatg ttgattacgc cgccgccgaa aatggggacg acgtctacag
    22141 cgcagcaacc gcaaattcag ccggtacagc agccacaaat tcaggctact caacaaccgc
    22201 aaatccagcc agtgcagcca gtagctcagc agccggtaca gatggaaaac ggacgcatcg
    22261 tctataaccg tcagtatggg aacattccga aaggcagtta tagcggcagt acctataccg
    22321 tgaaaaaagg cgacacactt ttctatatcg cctggattac tggcaacgat ttccgtgacc
    22381 ttgctcagcg caacaatatt caggcaccat acgcgctgaa cgttggtcag accttgcagg
    22441 tgggtaatgc ttccggtacg ccaatcactg gcggaaatgc cattacccag gccgacgcag
    22501 cagagcaagg agttgtgatc aagcctgcac aaaattccac cgttgctgtt gcgtcgcaac
    22561 cgacaattac gtattctgag tcttcgggtg aacagagtgc taacaaaatg ttgccgaaca
    22621 acaagccaac tgcgaccacg gtcacagcgc ctgtaacggt accaacagca agcacaaccg
    22681 agccgactgt cagcagtaca tcaaccagta cgcctatctc cacctggcgc tggccgactg
    22741 agggcaaagt gatcgaaacc tttggcgctt ctgagggggg caacaagggg attgatatcg
    22801 caggcagcaa aggacaggca attatcgcga ccgcagatgg ccgcgttgtt tatgctggta
    22861 acgcgctgcg cggctacggt aatctgatta tcatcaaaca taatgatgat tacctgagtg
    22921 cctacgccca taacgacaca atgctggtcc gggaacaaca agaagtgaag gcggggcaaa
    22981 aaatagcaac catgggtagc accggaacca gttcaacacg cttgcatttt gaaattcgtt
    23041 acaaggggaa atccgtaaac ccgctgcgtt atttgccgca gcgataaatc ggcggaacca
    23101 ggcttttgct tgaatgttcc gtcaagggat cacgggtagg agccacctta tgagtcagaa
    23161 tacgctgaaa gttcatgatt taaatgaaga tgcggaattt gatgagaacg gagttgaggt
    23221 ttttgacgaa aaggccttag tagaagagga acccagtgat aacgatttgg ccgaagagga
    23281 actgttatcg cagggagcca cacagcgtgt gttggacgcg actcagcttt accttggtga
    23341 gattggttat tcaccactgt taacggccga agaagaagtt tattttgcgc gtcgcgcact
    23401 gcgtggagat gtcgcctctc gccgccggat gatcgagagt aacttgcgtc tggtggtaaa
    23461 aattgcccgc cgttatggca atcgtggtct ggcgttgctg gaccttatcg aagagggcaa
    23521 cttggggctg atccgcgcgg tagagaagtt tgacccggaa cgtggtttcc gcttctcaac
    23581 atacgcaacc tggtggattc gccagacgat tgaacgggcg attatgaacc aaacccgtac
    23641 tattcgtttg ccgattcaca tcgtaaagga gctgaacgtt tacctgcgaa ccgcacgtga
    23701 gttgtcccat aagctggacc atgaaccaag tgcggaagag atcgcagagc aactggataa
    23761 gccagttgat gacgtcagcc gtatgcttcg tcttaacgag cgcattacct cggtagacac
    23821 cccgctgggt ggtgattccg aaaaagcgtt gctggacatc ctggccgatg aaaaagagaa
    23881 cggtccggaa gataccacgc aagatgacga tatgaagcag agcatcgtca aatggctgtt
    23941 cgagctgaac gccaaacagc gtgaagtact ggcacgtcga ttcggtttgc tggggtatga
    24001 agcggcaaca ctggaagatg taggtcgtga aattggcctc acccgtgaac gtgttcgcca
    24061 gattcaggtt gaaggcctgc gccgtttgcg cgaaatcctg caaacgcagg ggctgaatat
    24121 cgaagcgctg ttccgcaagt aagtaagcat ctgtcagaaa ggccagtctc aagcgaggct
    24181 ggcctttttc ttttgtttgg gaagcttgtg tttttgtcgc ctggataaga cacgtcagcg
    24241 tcctacccgg caatcagcat tctatgtgct catcatcttg cgcaccaact gcataaacag
    24301 ctcctgctct tctccactca aacgccccaa aaattcctca tccacgctgt caccaatagg
    24361 tatcgccgca gcaagtagct tttctccttc ggcagtcagc cagacaaagc gtcgccgctt
    24421 gtcagcgacg tcatgttcac gtctgactaa gccacgattt tccattcttg ccaacatttc
    24481 tgctagcgtt gctttggtgc tgactgctgc ttctatcagt gccacctgtt caataccagg
    24541 tttatcagca atggcgcgca tcaccgcgta ctgtggtttg gtcaggtcag gtaactcgtg
    24601 ctgccagcga gccgtgtgct gctgaaaaag ctgtcgtaac tgatggaacg ctttatttcg
    24661 taacgccatg taaactccgg gctaattctc tgtcgctatc atagcggttt tttagtgacg
    24721 aagaagatgt tgttactttt caatccttgc cgctgtggaa atgaaggagt atgttaataa
    24781 taacgttcgc atacgaataa ttaagagaat aagcaatgaa actgatcgtc gggatgacag
    24841 gggctaccgg tgcgcctctt ggtgtggcat tactgcaagc gctgcgtgag atgccgaatg
    24901 tcgagactca tctggtgatg tcgaagtggg cgaaaaccac cattgaactg gaaacgcctt
    24961 acagcgctcg cgatgttgct gccctcgcag acttcagcca taacccggcg gatcaggcgg
    25021 cgatcatctc atccggttct tttcgtaccg acggcatgat cgttattccg tgcagtatga
    25081 aaacgctcgc cggtatccgc gctggttacg ccgatggcct ggtagggcgc gcggcggacg
    25141 tcgtgctcaa agaaggccgc aaactggtgc tggtgccgcg tgaaatgccg cttagcacca
    25201 tccatctcga aaatatgctc gcactttcac gcatgggcgt ggcgatggtg ccgccgatgc
    25261 ctgcctttta taaccatccc gaaacggtag atgacattgt ccaccatgtg gtagcccgcg
    25321 tgctggatca atttggcctc gaacatcccc acgccaggcg ctggcaagga ttgccgcagg
    25381 cccggaattt ttctcaggag aatgaataat ggcatttgat gatttacgca gctttttaca
    25441 ggcgcttgat gaccacggcc agttactgaa aatcagcgaa gaagtgaacg ctgagccgga
    25501 tctggcagca gcagctaacg ccaccgggcg tatcggcgac ggcgcgcccg cgctgtggtt
    25561 tgataatatt cgcggcttta ccgatgcccg cgtggcgatg aataccatcg gttcctggca
    25621 gaaccacgcg atttcccttg gcctgccacc aaacaccccg gtcaaaaagc agattgatga
    25681 gtttatccgc cgctgggata acttcccgat tgccccggag cgccgcgcca atccagcctg
    25741 ggcgcagaac accgtcgatg gcgaagagat caacctgttc gatattctgc cgctgtttcg
    25801 cctgaatgat ggcgatggcg gtttctatct cgacaaagcg tgcgtggttt cccgcgatcc
    25861 gctcgacccg gataacttcg gcaagcagaa cgtcggcatc taccgcatgg aagtgaaggg
    25921 caagcgtaag ctcggcctgc aaccggtgcc gatgcacgat atcgccctgc atctgcataa
    25981 agcagaagag cgcggtgaag atctgccgat tgcgatcacg ctcggtaacg atccgatcat
    26041 cacgctgatg ggggccacgc cgctgaaata cgatcagtct gagtatgaaa tggcaggcgc
    26101 gctgcgtgaa agcccgtacc cgatcgccac cgctccgctg accggttttg atgtgccgtg
    26161 gggttcagaa gtgatcctcg aaggggttat cgaaagccgt aaacgcgaaa tcgaagggcc
    26221 gttcggtgag tttaccgggc actactccgg tgggcgcaac atgaccgtgg tgcgcatcga
    26281 taaagtctct tatcgcagca aaccgatttt cgaatcactc tatctcggta tgccgtggac
    26341 cgaaatcgac tacctatgac taggtaattt attcaagata accgggaaga gattaaaaaa
    26401 gacgggaata gattggaatc aacaagttag gaactcaagg ttttcaagat aaagcgagtc
    26461 caaaaatgtt aaacggttcg atatcaataa aacattcaag ataactcaca ggttcttaca
    26521 atagccttta aagaccgttc aaaaacgatt caggtgttta ttatagcgct gatgggtgca
    26581 agcgccaaaa ccaatgtaat tttagcacag ctaggcactt gcactgggtt atgcgctggc
    26641 tgctttctga ggtagattta agtgatcgcc cttagcttcg gacaacgcgt aaatctctct
    26701 aaccttttcg tctggcaaat taccaaggct taacgctatg gctctacgaa ctccaactag
    26761 agcgtgcaac tcttggatgt gctcatccag aagaatgagt aattgctccg ctccttttct
    26821 cccaagaaca tcagctactt gttgtttttc ttgtggttta agaaatgaca agaacattag
    26881 aatttgagca tcaacattat ccgcattctc ttttttgggt tctttggaat cttttagttc
    26941 aacatcaccc tctcccgtca tgagccattc caaggtaaca ttttcgcttt ctgctatctt
    27001 ttttaactga tacgggcgtg gtatcggtgc gtaatcccgc ctgttgtaat aattttttaa
    27061 agtgccttca ttgatccccc atgcccgagc agccgcattt cttgaagggt atcgttctac
    27121 taacctaaag attctttcga taatcggttc ttttccatcg gcaggaaaag aaccttgggg
    27181 tgcttttctc attcttggtt cctttctatc taattcattg ataaataata aaattaaaaa
    27241 ataaaccata aaaacaccaa agaacccttt gcagggaatc tttggtatct taatctttat
    27301 ccatacggat atccgaccgg atatccgcgc gggtaatttt gtaggttaaa ggaaagatga
    27361 gacaaagaga agatagtgac cgcgattggc cttcgctgaa gattcagctg gcgctcaaag
    27421 ataacgggtg gtcttccttc cgggagcttt cactccatgc tggttatgct caggattcac
    27481 tgaaagcggt agttcgcaca ccaaacaaaa aatacgaacg cgtaatttct gacgctatag
    27541 gtgtaccgcc agaaatcatc tggcccagcc ggtatcgtaa cgatggattt atcaggaagg
    27601 cgtcgtgata tgcagtcgta cgttaccgta aatgacctgc tcggcgtacc aggaatgcca
    27661 gccaccacaa aaggcatccg acaggcgtta cagcgttttt ctcgtgatct tggtgacgtt
    27721 agtcgccgtc gggaaggcac aaaagccatt gagtaccaca ttgattgtct gcctgaaata
    27781 acacgaaagg cactgcgtga acgctatgtg gaacagctgg tggctacaga gaacaatgtt
    27841 tctgaagtga aagctgttac cagaaaaaca cgcaatcctg acgctgtcca ggcaatcgaa
    27901 gcatatcgcg gttctccaca actgatggaa gaacgcctga atgcgctgac cgaaaaccag
    27961 cgctgggtat ctgaagcaag agctgcgctg gtggtggaag tgctgaagct ggaaagcgcc
    28021 ggtaaccccg ggcgactgaa agccattaac tttcttgttg aaaaagcccg taaaggtgag
    28081 ctgccggagc gcctgcaaca ggccgcagtt aacgccaatg caaaacgtgg cgctaatcgt
    28141 actatcagcc gcgatccact ttaccagtgg gttctgaaat acaaccagtc gcagaacgcc
    28201 gccgaacggc tgctgttact ggcaccggga aaacgcgacg aaataaaacc ggaagaaatc
    28261 agctggctgc ctgaatttct ggcgcagtat cgccaggtga acggcagacc catgtcagaa
    28321 gcctacgagg attttgtcgc agaatggcag cgtcgccatg cggacgaacc gtacatgctg
    28381 gaggtgatgc cttcctatga tgttgtccgg tatgccatga aaaaactgcc ggaagtggtg
    28441 aaacaaaaag ggcgcgttac cggcagtgaa taccgtcagc ttgaaggatt cacccgccgc
    28501 gactggacag ctatgccggt gaattatgtc tggattggtg acggtcacgg tatgaagctg
    28561 aaatgtgcgc atccgatcca cgggcgtcca ttctcaccgg aagtcacgtt tgtgatcgat
    28621 ggtggaacac ggtttgttgt tggctggagt cttgaccttg ccgaaaacgt gttcgccgtt
    28681 gccggtgcga tacagcacgg cattcgcaac cacggaaaac cgttcctgta ttactcggat
    28741 aacggttccg gtgaaacggc tgacatgctg gacaaagagg tcgtgggtat tctgccacgc
    28801 ctggggatta aacaccctac cggtattgcc ggtaatccac agggacgcgg cattattgaa
    28861 cgcctgaacc ggacactgcc gatgcgtata gccaggaggt atcgcaccta ttttggcaag
    28921 ggtgctgacc gggaatcgtt gcgtgtactt aaccgtgatc tgcgttcagc gtttaacgcc
    28981 ctgcaacagg acaagcctct gaacgaccgc cagaaagcgg caatgcgcga gctgccatcg
    29041 tgggcggagc ttattgaggc tattcgtgaa ggcgtggaat ggtacaacaa ccgcccgcat
    29101 tctgagctgc cgatgaaacc gaacggacgg cattacagcc cgacagagtt cagaaaaaaa
    29161 cgccaggcag aagaagacac cgaaattgag tggctgagtg atcttgagct gcgcgacatg
    29221 ttccgcccaa tggtggaacg accggtaaga cgctgcgaaa ttcagtggct gaacaatatt
    29281 tactacgcgc cggagttgcg tgacgaacac ggtcgcaaag tgcttatcag ttatgacatt
    29341 catgacgccg agcgaattac ggtacgtcgc aaggacggca gctttatctg tgaggcgata
    29401 tggaacggca ataaacgcgc agcctttgct gtcagtgctg aatatcacaa acaacagcag
    29461 cgtattaaag gaatgcgcaa acgcgctgag gaaaaaattc gtgatgctga agatgaaggt
    29521 attcagattc tggaacacaa acaggctgaa ccctggctga gtaatgttta tcgcccggtt
    29581 ggtaatgtgg tggcagttca gcaaccggaa tatgaagaag agcatgacga agaatttgaa
    29641 cgcgatttcc ggctggggat gcaaaaactt tttgccatgc aggaagaaga tgatccgctg
    29701 gcctgaataa aaaaacagtc cgcgggaacg gactgttaaa taaaggaaac aaagctgatt
    29761 tgaaataacc gaggaaatac tatgactg
//
LOCUS       DAJTRD010000043        29476 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTRD010000043 DAJTRD010000000
VERSION     DAJTRD010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29476
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.043"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(1..441)
                     /locus_tag="OO315_002120"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="OO315_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO315_002121"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO315_002121"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5891034.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO315_002122"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO315_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5891035.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO315_002123"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO315_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5891036.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO315_002124"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO315_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="OO315_002125"
     CDS             5754..6257
                     /locus_tag="OO315_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5891037.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /locus_tag="OO315_002126"
     CDS             complement(6337..6588)
                     /locus_tag="OO315_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5891038.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO315_002127"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO315_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5891039.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="OO315_002128"
     CDS             7052..7375
                     /locus_tag="OO315_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5891040.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="OO315_002129"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="OO315_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5891041.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="OO315_002130"
     CDS             complement(8080..8319)
                     /locus_tag="OO315_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5891042.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="OO315_002131"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="OO315_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5891043.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO315_002132"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO315_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5891044.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="OO315_002133"
     tRNA            complement(10643..10729)
                     /locus_tag="OO315_002133"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="OO315_002134"
     tRNA            complement(10742..10815)
                     /locus_tag="OO315_002134"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="OO315_002135"
     tRNA            complement(10870..10945)
                     /locus_tag="OO315_002135"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO315_002136"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO315_002136"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5891045.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO315_002137"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO315_002137"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5891046.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO315_002138"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO315_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5891047.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14483..14659
                     /locus_tag="OO315_002139"
     CDS             14483..14659
                     /locus_tag="OO315_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891048.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /locus_tag="OO315_002140"
     CDS             14646..14870
                     /locus_tag="OO315_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5891049.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO315_002141"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO315_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO315_002142"
     CDS             complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO315_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5891050.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO315_002143"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO315_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5891051.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO315_002144"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO315_002144"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5891052.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO315_002145"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO315_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5891053.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO315_002146"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO315_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5891054.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO315_002147"
                     /pseudo
     CDS             complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO315_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO315_002148"
     CDS             complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO315_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5891055.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="OO315_002149"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="OO315_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5891056.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="OO315_002150"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="OO315_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5891057.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="OO315_002151"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="OO315_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5891058.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="OO315_002152"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="OO315_002152"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5891059.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /locus_tag="OO315_002153"
     CDS             complement(26368..26781)
                     /locus_tag="OO315_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5891060.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26968..28173
                     /gene="yedE"
                     /locus_tag="OO315_002154"
     CDS             26968..28173
                     /gene="yedE"
                     /locus_tag="OO315_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5891061.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28170..28403
                     /gene="yedF"
                     /locus_tag="OO315_002155"
     CDS             28170..28403
                     /gene="yedF"
                     /locus_tag="OO315_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5891062.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28510..29180
                     /locus_tag="OO315_002156"
                     /pseudo
     CDS             28510..29180
                     /locus_tag="OO315_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29220..>29476)
                     /locus_tag="OO315_002157"
     CDS             complement(29220..>29476)
                     /locus_tag="OO315_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5891063.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         7338 a         7613 c         7017 g         7508 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcaactcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatggcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca
    26941 aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg
    27001 attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat
    27061 tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag
    27121 ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg
    27181 gaaggatcgc cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc
    27241 tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc
    27301 attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc
    27361 tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt
    27421 gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc
    27481 cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat
    27541 caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg
    27601 ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt
    27661 ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc
    27721 accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg
    27781 atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg
    27841 gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc
    27901 tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg
    27961 atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac
    28021 tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat
    28081 ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt
    28141 gaggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg
    28201 atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat
    28261 taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc
    28321 cactggatgc gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca
    28381 ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg
    28441 ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag
    28501 gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt
    28561 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat
    28621 gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat
    28681 ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc
    28741 gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    28801 tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta
    28861 tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc
    28921 gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag
    28981 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc
    29041 aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc
    29101 agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt
    29161 taattcaacc tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt
    29221 caggctgtcg cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc
    29281 atctctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc tccatttaca
    29341 tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt
    29401 tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cggaacagca
    29461 agcacctgac cgccac
//
LOCUS       DAJTRD010000044        29464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTRD010000044 DAJTRD010000000
VERSION     DAJTRD010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.044"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            68..349
                     /locus_tag="OO315_002158"
     CDS             68..349
                     /locus_tag="OO315_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5891064.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
     gene            315..1392
                     /locus_tag="OO315_002159"
                     /pseudo
     CDS             315..1392
                     /locus_tag="OO315_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(1785..2126)
                     /locus_tag="OO315_002160"
     CDS             complement(1785..2126)
                     /locus_tag="OO315_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5891065.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(2139..3011)
                     /gene="copD"
                     /locus_tag="OO315_002161"
                     /pseudo
     CDS             complement(2139..3011)
                     /gene="copD"
                     /locus_tag="OO315_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            complement(3015..3389)
                     /gene="yobA"
                     /locus_tag="OO315_002162"
     CDS             complement(3015..3389)
                     /gene="yobA"
                     /locus_tag="OO315_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5891066.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3528..3758
                     /gene="holE"
                     /locus_tag="OO315_002163"
     CDS             3528..3758
                     /gene="holE"
                     /locus_tag="OO315_002163"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5891067.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3860..4516
                     /locus_tag="OO315_002164"
                     /pseudo
     CDS             3860..4516
                     /locus_tag="OO315_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            4540..5202
                     /gene="exoX"
                     /locus_tag="OO315_002165"
     CDS             4540..5202
                     /gene="exoX"
                     /locus_tag="OO315_002165"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5891068.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(5199..7259)
                     /gene="ptrB"
                     /locus_tag="OO315_002166"
     CDS             complement(5199..7259)
                     /gene="ptrB"
                     /locus_tag="OO315_002166"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5891069.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7469..8128)
                     /locus_tag="OO315_002167"
     CDS             complement(7469..8128)
                     /locus_tag="OO315_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5891070.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            complement(8455..8811)
                     /locus_tag="OO315_002168"
     CDS             complement(8455..8811)
                     /locus_tag="OO315_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5891071.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            complement(8878..9168)
                     /gene="yebG"
                     /locus_tag="OO315_002169"
                     /pseudo
     CDS             complement(8878..9168)
                     /gene="yebG"
                     /locus_tag="OO315_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            9302..10480
                     /gene="purT"
                     /locus_tag="OO315_002170"
     CDS             9302..10480
                     /gene="purT"
                     /locus_tag="OO315_002170"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5891072.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10536..11177)
                     /gene="kdgA"
                     /locus_tag="OO315_002171"
                     /gene_synonym="eda"
     CDS             complement(10536..11177)
                     /gene="kdgA"
                     /locus_tag="OO315_002171"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5891073.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(11214..13025)
                     /gene="edd"
                     /locus_tag="OO315_002172"
     CDS             complement(11214..13025)
                     /gene="edd"
                     /locus_tag="OO315_002172"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5891074.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(13260..14735)
                     /gene="zwf"
                     /locus_tag="OO315_002173"
     CDS             complement(13260..14735)
                     /gene="zwf"
                     /locus_tag="OO315_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5891075.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            15073..15942
                     /gene="hexR"
                     /locus_tag="OO315_002174"
                     /gene_synonym="yebK"
     CDS             15073..15942
                     /gene="hexR"
                     /locus_tag="OO315_002174"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5891076.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            16070..17512
                     /gene="pyk"
                     /locus_tag="OO315_002175"
     CDS             16070..17512
                     /gene="pyk"
                     /locus_tag="OO315_002175"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5891077.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17643..18614)
                     /gene="lpxM"
                     /locus_tag="OO315_002176"
                     /gene_synonym="msbB"
     CDS             complement(17643..18614)
                     /gene="lpxM"
                     /locus_tag="OO315_002176"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5891078.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(18734..20056)
                     /gene="mepM"
                     /locus_tag="OO315_002177"
     CDS             complement(18734..20056)
                     /gene="mepM"
                     /locus_tag="OO315_002177"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5891079.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(20072..21004)
                     /gene="znuA"
                     /locus_tag="OO315_002178"
     CDS             complement(20072..21004)
                     /gene="znuA"
                     /locus_tag="OO315_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5891080.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            21083..21838
                     /gene="znuC"
                     /locus_tag="OO315_002179"
     CDS             21083..21838
                     /gene="znuC"
                     /locus_tag="OO315_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5891081.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            21835..22620
                     /gene="znuB"
                     /locus_tag="OO315_002180"
     CDS             21835..22620
                     /gene="znuB"
                     /locus_tag="OO315_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5891082.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(22767..23777)
                     /gene="ruvB"
                     /locus_tag="OO315_002181"
     CDS             complement(22767..23777)
                     /gene="ruvB"
                     /locus_tag="OO315_002181"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5891083.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(23786..24397)
                     /gene="ruvA"
                     /locus_tag="OO315_002182"
     CDS             complement(23786..24397)
                     /gene="ruvA"
                     /locus_tag="OO315_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5891084.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            complement(24536..24601)
                     /gene="yobI"
                     /locus_tag="OO315_002183"
     CDS             complement(24536..24601)
                     /gene="yobI"
                     /locus_tag="OO315_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5891085.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            24671..25273
                     /locus_tag="OO315_002184"
     CDS             24671..25273
                     /locus_tag="OO315_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5891086.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(25275..25796)
                     /gene="ruvC"
                     /locus_tag="OO315_002185"
     CDS             complement(25275..25796)
                     /gene="ruvC"
                     /locus_tag="OO315_002185"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5891087.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(25831..26571)
                     /locus_tag="OO315_002186"
     CDS             complement(25831..26571)
                     /locus_tag="OO315_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5891088.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(26600..27052)
                     /gene="nudB"
                     /locus_tag="OO315_002187"
     CDS             complement(26600..27052)
                     /gene="nudB"
                     /locus_tag="OO315_002187"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5891089.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(27170..28942)
                     /gene="aspS"
                     /locus_tag="OO315_002188"
     CDS             complement(27170..28942)
                     /gene="aspS"
                     /locus_tag="OO315_002188"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5891090.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            29252..>29464
                     /locus_tag="OO315_002189"
     CDS             29252..>29464
                     /locus_tag="OO315_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5891091.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVD"
BASE COUNT         7224 a         7568 c         7237 g         7435 t
ORIGIN      
        1 taaaaatcaa ccacacccgc cagtattctg tatatttact ggcggtcata tcgtgagagg
       61 tatggcaatg aatcttgtga cactcaaaac gtggggaaaa ctcagatatc cggataaccc
      121 accatcaata tcaacgctga gacgatgggc aaggaatgga aacatttatc ctgcacctga
      181 actacacggg aggagttaca gggtggttcc ggaggctttc tatatcaacc caaataaggt
      241 tgataccgat ataacacacc atcagcctaa tgggcgacaa gggagagaca gtccgttaat
      301 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
      361 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      421 taggtaaaat ttcaagaaga gatgcagtag cgcaggcaat tgaagcaaat cattatatat
      481 acaaaaacta ctctcctgct gccctaattg aaaagcttaa aggggtcgac tcatttacta
      541 tggcaggctg gattgaacgt tacaaaacga ttcttataag gagaaaagcg tccagaaata
      601 cttataaaac tcgggtaaat caactggaga caataaaaga aaaattagga ggggttttac
      661 tgacagaaat aaccactcgc catatcgccg agtttcttga tttgtagatt gaaggaggga
      721 aaaacacgat ggcaggatca atgcgttctg tgttatctga tatgttccgc gaagccattg
      781 ttgaaggacg tatatctcaa aatccagtaa cgccaacaag agcaccgaaa atagtagtta
      841 caagagaacg gctgaaacta aagacataca actgcatcag ggaggcagca gatcaacttc
      901 cggcatggtt cccattagct atggacttag cccttgtaac aggacaacgt cgcgaagaca
      961 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacatcagg caaattaaga
     1021 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ttacgacttg
     1081 gtgcagtagt tgaacagtgc cgcatggtaa gtagggggga ttatctaatc agtgccggga
     1141 ttagaaaaaa cagccctgac ggcagcattc acccggacgg cctgacaaag aaatttgtcg
     1201 cagccagaaa attaacaggt atcactgttc agtgaaaacc caccaacttt tcacgaaatc
     1261 agaagcctgg ctggacgatt gtacaaagaa atatgtggag aagaatttgc tcagcgtcta
     1321 cttggccaca catcggagaa gacaacaaaa atgtatcttg atgagagaga aaaaacgtac
     1381 ttactgctct gattttaacg taaatggatt gttaaatgta ttttggttgt gatataacca
     1441 aaaaagaccg gaatacagaa attcgagtaa atttcgggga atttcgggga gacgtttgca
     1501 actaattgat tttaaataca attaaaaaaa gaccgaatac gattcctgta ttcggtccag
     1561 ggaaatggct cttgggagag agccgtgcgc taaaagttgg cattaatgca ggcttagttg
     1621 ccttgccctt taagaataga tgacgacgcc aggttttcca gtttgcgtgc aaaatggtca
     1681 ataaaaagcg cggtgctcat cagcttaaat gttaaaaacc gcccgttctg gtgaaagaac
     1741 tgaggcggtt tttttattgg aaatcaaaag gctattttag gtaattaaca gagtttttca
     1801 gctcgttcta taaacggtgc cagactcatt ttttcgccgg gattgttagg atcatcaatc
     1861 tgaatcaccg aaatgggttg ggcattggtc ttcccactgg caacttcctt ttgtgcgata
     1921 tcgtttaaag gatactgcac gagggtactt gggttgatga catacaaagc attacccggt
     1981 cggcaagtca gcattacctc ttcgcgatta aacgcccatt tgtctttacc cacttcaaaa
     2041 cgactcacgg ttatcacctg tggagccgcc agcgccgctg cagaactggt gagtaacaga
     2101 aacgccagaa tacttttttt catcattttc gtaatccatc aaaaaggttc gagagtcgca
     2161 agcaggctga tgatcaccag cactaccgca cctatcgccc attcgagttt agtcatccac
     2221 acaaaccagg gtgccgcacg atcttcatcc tgcctcatgc gcggtacaag aacataacga
     2281 ttagccagcg caatgaccac cataatcatc accaggatgg ctttgagcaa taagagttgg
     2341 ccccagtaag tcgtaagcgt ggggggaaat ccggtaatca acaatgcatt aagcacgccg
     2401 cttgccagta cgccgatcac cgcaaaatgc ccgcaccagg aaaaacgcat cagcgcctga
     2461 atagcctagt gtcgccaacg gcctttgatg agttgcatac accagagcac cggcagtaat
     2521 cccccaaacc acgccgccgc acaaatcagg tgaatcgcgt gattagtctg atggattttc
     2581 gctgttaccc cttcattcag cgtcgcatac cccactcccg ccagcagaat aaattgcgcg
     2641 gtagtgagca taaacagcaa acgtggcata ttccgcggtt gcataagggc gacgatcaac
     2701 gttacgaggg cgagaacaat ttgccataac cagacgccac caaactgcgt ttgcaaaaca
     2761 gcctgccaaa tgtttggcga aaatacatct gtccatcccg ttcccatcaa cccgccctga
     2821 acggcgagca ttgcagtggc actgatcaaa ctccagacgg cagcgtgctg ttgtaaacgc
     2881 agagaacgct tcgtcagcaa gcgacgaatc attaaggggg ccagccaggc accatacatt
     2941 gcaaagccaa agaccagcat caacgaggtg aaatggataa atcgcagcgc gatccaggta
     3001 aacgccagca tgatttattt cacgctaaag gtgtagtgtc ctttcgtttt atgcccgtcc
     3061 accgaaacaa catgccagtc aacggtgtag gttccgggtt tcagcgaatc ggccagtgga
     3121 acaatcagtt gcttttggtc ctgctcattc cgcttcgctg gtaatgtttt aatattttcg
     3181 ttttttggcc ccgtgatttt tgcaccactg aatcctgttt caacgccttc cgagaagttt
     3241 aaagtgattg cctgcggggc agctgtcact tgcgcgtttg ccgcaggata ctgatgcgtt
     3301 aaatgcgcat gtgcccagac ggaaggtgtc accaacgaag tggtcaggat tgccagcgcg
     3361 tagcgaaggg agcgtgcagt tgaagccata ttatctattc ctttttgtaa taactttttt
     3421 acagagcata accttgtcta atgtctgagt cgaggatcat caattccggc ttgccatcca
     3481 ggctcactct tagtaacttt tgcccgcgaa tgatgaggag attaagaatg ctgaagaatc
     3541 tggctaaact ggatcaaaca gaaatggata aagtgaatgt cgatttggcg gcggccgggg
     3601 tggcttttaa agaacgctac aatatgccgg tgatcgctga agcggttgaa cgtgaacagc
     3661 cagaacattt gcgcagctgg tttcgcgagc ggcttattgc ccaccgtttg gcttcggtca
     3721 atctgtcacg tttaccttac gagcccaaac ttaaataaaa cttatacaga gttacacttt
     3781 cttacataac gcctgctaaa ttatgagtgt tttctaaacc gcccccataa tttgcagtca
     3841 ttttgaaaag gaagtcatta tgtcgttctg gaaagttgca gcagcgcaat atgagcctcg
     3901 taaaacctca ctcaccgaac aggtagctca tcatctggag tttgtgaggg ccgccgccag
     3961 gcaacaatgt cagctgttag tttttccatc gctttcttta ctggggtgtg attattcgcg
     4021 acgtgccctt cctgccccac ccgatctttc actgttagac ccgctttgtt atgccgcaac
     4081 gacctagcga atgaccatca ttgccggcct tcccgttgaa tataacgatc gctttattcg
     4141 tggaattgcg gtgttcgcac cgtggcgaaa aacgcctggg aactaccatc agagtcatgg
     4201 tgcatgtctg ggccgtcgtt ccagaaccat cacggtggtc gatgaacaac cgcaaggtat
     4261 ggatatggac ccgacctgtt cactgtttac gacagggcaa tgtctgggag aacccgacct
     4321 gctggcgtcg gcccgtcgct tacaattttt ttcacatcag tactcgattg ccgtgctgat
     4381 ggcaaatgcc cgtggtaaca gtgcactatg ggatgaatat ggtcgtctca tcgttcgcgc
     4441 cgatcgtggt tcattattgt tagtcggtca gcgttcatca cagggttggc aaggcgatat
     4501 cattccatta cgctaggctt tttcggcctg gatcatgcca tgttgcgcat tatcgataca
     4561 gaaacctgcg gtttgcaggg agggatcgtt gagattgcct ctgttgatgt cattgacgga
     4621 aaaatcgtca accccatgag cctcctggtg cgccccgatc gtcctattag tccacaagcg
     4681 atggcgattc atcgcatcac cgaagccatg gtcgccgata aaccgtggat tgaagatgtg
     4741 atcccacact attacggtag tgaatggtat gtcgcgcaca acgccagctt tgaccgccgc
     4801 gtactgcctg agatgcccgg tgagtggatt tgcactatga aactggcccg tcgtttgtgg
     4861 ccagggctca agtacagcaa tatggcgtta tataaaacac gcaagctcaa tgtacagaca
     4921 ccgctgggcc tgcatcatca ccgcgcgttg tatgactgtt atatcaccgc cgcgttgctt
     4981 atcgatatta tgaacacctc cggctggacg gcagaacaga tggccgatat caccggacgt
     5041 ccgtcgttga tgacaacctt cacctttggc aaataccgtg gcaaagcggt ttccgacgtt
     5101 gccgaacgct atccgggcta tctgcgctgg ttatttaata acctggacag catgagcccg
     5161 gagctgcgtt taacactgaa acattatctg gaaaatactt aatccgcagg cgtagcgggt
     5221 aatgttccct gcgccagcgc gaccagaaaa gcatattcca tcgctacgcc ttcgtacgat
     5281 ttaaagcggc cagatttacc gccatggcct gagtccatgt cggtacagag caataaaaga
     5341 tggtcatcgg ttttcagctc gcgcaattta gcgacccatt ttgccggttc ccaatattgc
     5401 acctgagaat cgtgtaaacc ggtcgttacc agtaaatgcg gataagcctg tgcggtgacg
     5461 ttgtcatacg ggctgtagct tttcatgtac tcgtaatatt gcggatcctg cggatttccc
     5521 cactcttcaa actcaccagt ggtaagagga attgattcat caagcatcgt tgttacaaca
     5581 tcaacaaacg gtacctgggc gataacgccg tggaataatt ccgggcgttg attaattgca
     5641 acgcccatca acatgccccc cgcacttccg cccatcgcat aacagagcga aggagagcca
     5701 tagcccagtt ttaacaatgc atcgcaggca tcaagataat cattaaacgt atttttcttc
     5761 ttcagaaatt taccgtcttc gtaccattgt tgccccagct caccaccgcc gcgaacatgg
     5821 acaatggcgt agacaaagcc acgatctaac aaactcaagc ggctaaaact gaaatcggca
     5881 tcaatacttg cgccgtaaga accatagcca tacaccagca acgggttgtg tcctttgcga
     5941 aaatgtttgc gatgatagac cagcgaaacc ggaacttcga cgccatcacg ggcgactatc
     6001 cacaggtgtt cactgcggta attcgccgca tcaaaaccag gcacttctgt ttgttttaat
     6061 acacgacgct caccggtatc catatccagt tcaaacaaag tgtctggtgt ggtcatggaa
     6121 gaataaccat aacgcaatcg cgcggtttca ggttctggat tgtaggcaat ccaggtcaca
     6181 taggccggat catcaaaggc aatgccaatg acttcccggg tcttgcggtt aatttggcgc
     6241 aaactggtta acccgcgctg acgctcttca accaccagcc agtcggtaaa cagcgtaaac
     6301 ccttccagca tgatgttttc gcgtggcgga attaactctt cccactgttg ctcatcacgc
     6361 atacgggtac ggtataagcc aaagtttttg ccgtggcgat tggaacgcag ataaaaacga
     6421 tgctggtagt gatcaaggct gtattcgtga tctttgcggc gcggcagaaa aacaaacggc
     6481 tcggcatcgg ccatttccgc gtccagcagg cgaacttcac tggtggtggc gctggccaga
     6541 tgaatgacga catagtgctt cgacgtcgtt ttatgcaggc tgacataata ggtatcgtct
     6601 ttttcttcgt agatcagtgt atcttgcgat gctggcgtac cgatggcgtg acgccagacc
     6661 tgataaggca gcagcgtcac cggatgcttg cgaacatagt agaaagtcca ggagtcattt
     6721 gcccagacaa agctgggttc aacgttatcc agcagttccg ggtaccagtt acctgtttcc
     6781 agattacgaa aacgaatgcc gtactggcgt cgggaaagaa aatcttctgc cagcgccata
     6841 atggtgttat cgggcgtaat cgccattccg cccatcgaat aaaactcact atgagctgcg
     6901 cgcttgttgg catcgagcaa tgtttcccac tcatcccact cttcgctgaa tgccgattga
     6961 cgctggtaga tagcatattc acagcctggt tcataaatat gccgatagcg gtagccattt
     7021 tttatgtagg gcgcagaaac ttctctttgc ggaatgcggt cgatgatttc ctttaagatg
     7081 cgatcctgca aggcttgttg tgaggccatc acccgatgac cgtaactatt ttcttgttgc
     7141 aggtagtcca ggacttttgg ctgagaacgc gtatcgtccc gcagccagta gtaattatcg
     7201 atgcgcgtat cgccatgaag cgtcatggcg tggggaatgc gggcggcttt tggtagcatg
     7261 ttattgttct ttctggttgg aaacatctta taagggtggc aaaactcacc ggggatgcaa
     7321 gcgaaacagg gtcgtcattg cttagatgat gacaggtaat ggcgcggata tcgaatgtta
     7381 tgcagacaga gaaaatcagc ctgttcaaat ggctgtgcgg ttctggatag cccgaaatag
     7441 tcaacttcag gctatccaga gagcggaatt attccgccaa agtgcgtttt tgctgttcga
     7501 gatcgcgttc aatgccttca cgaacatcct gggggatttt cagcgcgtca cccagtgcat
     7561 tcaggtaact gcgttccata aaatggtcaa tatcaatagc cgcgcagctc aggaaataga
     7621 tttccagcgc ctcttcttca ttgcggaccc cggtagccag gcgttgtgga tccagcggtt
     7681 gttcgattgc ctgctcaatg agtacacgcc cctgctcttc cacgccggct tcacgcaatt
     7741 gctggtcgat tgccgcacgt tcattggcat caatatgacc atcactttta gcggcaaaga
     7801 ccagcgcaag gatcaaacgt tccgtacgct catccagcgg cgtactttgc gcgccaaact
     7861 gcggttcgtc ctgatgcgcc gcgcgaattt tatctttgta tttattccac agcaccgtac
     7921 ccgctaccgc tccgccgcca accagtaacg cgttggtgcc atatttcgta agaagtttac
     7981 gtgctgattt atttgcgacc agcagcccag ccagtccgcc taatgcgcct gacactaaca
     8041 gtttgcccaa tccctgatcc gcagacgagg aggtagaaga actgctttgc ccaagcaggg
     8101 attgcagttg atttaaccag ttagccatat ttgctcctca ataaccattt ttagtgcctg
     8161 tagcatagcg gatgaggatg tcagaaaatg tatatcgaga caaaagatgc gcaaataagc
     8221 ggggcggtag gccgttattc aaagaaattc gcgccaggta aggtatttac tgtatatcag
     8281 gtgataaggg tattttaggt gaaagtagtt tgtcgttttg agtggcattg tttgatgctg
     8341 attgcccgat gcgacgctgt tgcgtcttct caggcctacg attgtggtgc gttctgggac
     8401 ggacaaggca ctcgcgccgc atccgccaac ggtcaatcga attatcagaa gccattaacg
     8461 ccgctgatat tccgccattc ccgctttgca gtccacgcag acctggtaat gaatatcggc
     8521 acttttacca cgcacggtta gcggtactga ccatttatca tctttaccct gaatgtcctg
     8581 caaactgacc caggccacgg gatcggcctg accgacaatt ttttgatcat ctgcccagcg
     8641 cgccacgcga ttttgttgat aatcacgttt tacgctcgcg gcaattccgg cagcatccag
     8701 accttcacac tttgggaaag tgaccgactt gctggtttca ttattggcag cgaaaactga
     8761 tgcgcaggca gaaaccaaca accgccctaa aaacgcccct ctttttttca tgtttttctc
     8821 catagcacaa tgattcagga gaaagcatgg tacaaattgt caggagcgca agttgcttca
     8881 ggccgcgtgt gaggcatctt cctcttcgga ttcagcaccg acgacctgtg gggacggtaa
     8941 tttgccggtt ttcagaatgg tgctcagcac ctctttttgt tctgccagcc atagcgaaag
     9001 ggcttcacgt tgctcgtctt ccatttgcac tggagaattt gtcagctagg tgtcaagcag
     9061 atccgccgta tcaagcattt tgtcataagc atcggcttcc tttttgctgg taaacgacat
     9121 tttctcttcg ccctcacgaa tgactacgta tttaacttca accgccattt gcagcctctc
     9181 ataataactg agattttata cagtatattt cttttcggtt gagaaatcaa catcagcaat
     9241 aaagacacac gcaaacgttt tcgtttatac tgcgcgcgga attaatcagg ggatattcgt
     9301 tatgacgtta ttaggcactg cgctgcgtcc ggcagcaact cgcgtgatgt tattaggctc
     9361 cggtgaactg ggtaaagaag tggcaatcga gtgtcagcgt ctcggcgtag aggtgattgc
     9421 cgtcgatcgc tatgccgacg caccagccat gcatgtcgcg catcgctccc atgtcattaa
     9481 tatgcttgat ggtgatgcat tacgccgtgt ggttgaactg gaaaaaccac attatatcgt
     9541 gccggagatc gaagctattg ccaccgatat gctgatccaa cttgaagagg aaggactgaa
     9601 tgttgtcccc tgcgctcgcg caacgaaatt aacgatgaat cgcgagggta tccgtcgcct
     9661 ggcggcagaa gagctgcagc tgcccacttc cacttatcgt tttgccgata gcaaaagcct
     9721 tttccgcgag gcggttgccg ccattggcta tccctgcatt gtaaaaccgg tgatgagctc
     9781 ttccggcaag gggcagacgt ttattcgctc tgcagagcaa cttgctcagg catgggaata
     9841 cgctcagcaa ggcggtcgcg ccggagcggg ccgcgtaatt gttgaaggcg tcgttaagtt
     9901 tgacttcgaa attaccctgc taaccgtcag cgcggtggat ggcgtccatt tctgtgcacc
     9961 agtaggtcat cgccaggaag atggcgacta ccgtgaatcc tggcaaccac agcaaatgag
    10021 cccgcttgcc cttgaacgtg cgcaggagat tgcccgtaaa gtggtgctgg cactgggcgg
    10081 ttatggtctg tttggtgtcg agctgtttgt ctgtggtgat gaggtgattt tcagtgaggt
    10141 ctcccctcgt ccacatgata ccgggatggt gacgttaatt tctcaagatc tctcagagtt
    10201 tgccctgcat gtacgtgcct tcctcggact tcctgttggc gggatccgtc agtatggtcc
    10261 tgcagcttct gccgttattc tgccacaact gaccagtcag aatgtcacgt ttgataatgt
    10321 gcagaatgcc gtaggcgcag atttgcagat tcgtttattt ggtaagccgg aaattgatgg
    10381 cagccgtcat ctgggggtgg tactggctac ttcagagagt gttgttgacg ccattgaacg
    10441 cgcgaagcac gccgccggac aggtaaaagt acagggttaa acccggcaaa aaaacgctac
    10501 aaaaatgccc gatcctagat cgggcatttt gacttttaca gcttagcgcc ttctacagct
    10561 tcacgcgcca gcttagtaat gcggtcgtaa tcgcccgctt ccagcgcatc tgccggaacc
    10621 agccaggaac caccgatgca cagcacgctt ttcagtgcca ggtagtcacg gtagttagcc
    10681 ggagaaatac cacccgtcgg gcagaaacgg acctgggaga acggacccgc gatcgcctgc
    10741 agggctttca cgccgccgtt agcttcagcc gggaagaatt tgaactcttt caaaccgtag
    10801 tccataccca gcatcagttc ggaaacagtg ctgatccccg gaatcagagg aatagtccct
    10861 tcggtagcag ctttcagcag cggctcggtc agacccgggc taattgcgaa ctgtgcaccc
    10921 gcttcagtga cttctgccag ctgctgtgga ttcagcaccg taccggcacc cacaatcgct
    10981 tcaggcactt ctttggcgat agcacggata gcgtcaactg cacactcggt acgcagagtc
    11041 acttccagaa cgcgcacccc accagcaacc aacgcttttg ccatcggcac cgcgtgttcc
    11101 agttttttta ccacgataac cggtacaacc gggccggtgg tcaggattga ttctgcactt
    11161 gttttccagt ttttcatcag agttttctct cgcctgatta caaatttgtc gtcttaaaaa
    11221 gtgatacagg ttgcgccctg ttcggcaccg gacagttttt cacgcaaggc gctgaataat
    11281 tcacgtcctg ttcccacgcg tgacgcgctc aggtcaggaa tgtgcggttc gcgagcagcc
    11341 agttccgctt cgtctaccag cagtgtcagt tcgcctgtct gtccattcac acgaatgatg
    11401 tccccgtcgc gcacttttgc cagcagcccg ccatcgtagg cttctggtgt tacgtggata
    11461 gctgacggca ctttacctga agcgccggag agtcgtccat cggtaactaa cgcaattttg
    11521 aaacaccggt ccaataatac accaagtggc ggcatgagtt tatgtaattc tggcattccg
    11581 ttcgcttttg gcccctgatg acggacaaca acgacacaat cgcggtccag caaacccgct
    11641 tcaaaggccg gcataacgtc atgctggctt tcaaaaacaa ccgctggcgc ttcaatcacc
    11701 tggttctcaa ccggcacggc agaggttttc ataaccgcac ggcccaggtt accgcttaac
    11761 acttttgtcc caccatgatg agagaaaggt tgttcgaagg aagcgatcac attgctgtcg
    11821 agtgattttt ccgccccttc ccgccagtcc agttcaccat tattcagcca tggttcaagg
    11881 gtataacgag acagaccaaa gcctgccacc gtattgacat cttcatgcag caggcctgct
    11941 ttgagcagtt cacgcaccag aaccggtacg ccacctgccg cctggaagtg gttaatatcg
    12001 gccggaccgt tcgggtagag acgtgccatc agcggtacaa catcagaaag gtcagagaag
    12061 tcatcccagt taatctgaat accggccgcg cgcgccatcg ccaccaggtg catggtgtgg
    12121 ttagtggaac caccggtcgc cagcagtgca acgataccgt tcaccaccac tttctcatcg
    12181 atcatcttac cgatcggcat ccattcatta ccattaccgg tcatgcgtgt aacctgacgc
    12241 gcagcggcag cggtcaaagc atcgcgcagc ggagaatccg gatgaacaaa agaagagcct
    12301 ggcaactgca tccccataaa ttccaccacc atctggttgg tgttggcagt accgtagaaa
    12361 gtacatgttc ccggcgcatg gtaagacgcg gcttctgact ccagtaaggc catgcggtcc
    12421 actttacctt cggcataaag ctggcgaata cgcacttttt ctttatttgg caaaccgctt
    12481 gccatcggtc cagacggcac aaacaccgca ggcaaatgac caaacgacag ggctgccatc
    12541 gtcagacccg ggacaatctt gtcgcacaca ccgaggaaca gagcaccatc aaacatgtta
    12601 tgggacagcc ccaccgccgc agacatcgct atcacttcgc ggcttagcag cgacaattcc
    12661 attccatcct gcccctgggt gacaccatca cacatcgccg gaacaccgcc cgcaacctga
    12721 ccaaccgcat tcgcttcatg cagggcttta cgaatgattt ctggatagtg ttcataaggc
    12781 tggtgcgcgg agagcatgtc gttataggag gtgatgatgg cgatattgtt acgcaacatg
    12841 cttttcaaag aggctttgtt ttctggctgg caggcagcga aaccgtgtgc caggttaccg
    12901 catgccaact gcgaacgatg aacggtcgac gttttcgctt gttctatccg ggcgagataa
    12961 gcagagcgag tctcgcgcga acgttcaatg attcgatttg ttacgcgtaa caattgtgga
    13021 ttcataaagg ctcctgaaat tgagttgtca gagcaggatg attcacaacg cgtttcattc
    13081 agaggattta tgagtgaaac gcctgtaacc ggagctcata gggcaaaaac gtttcagtca
    13141 gtgtaataaa aaaagcctcg tgggtgaatc cgcacgaggc ctgaaagtgt aaaaattgtt
    13201 ctacaatctg tgcaagatca tgttaccggt aaaataacca taaaggataa gcgctgatat
    13261 tactcaaact cattccagga acgaccatca cgggtaatca tcgccaccga ggcaacgggt
    13321 ccccaggttc cggcctgata cggtttcggc gcatcattgt ccatcgccca cgcctcagta
    13381 atggagtcta cccatttcca ggcttcttcc acttcgtcgc gacgtacaaa cagtgcctga
    13441 ataccacgca tggtttccag cagcaaacgt tcataggcat ccgccagatg cgtctgatta
    13501 aaggtttctg aatagctcag atccagcttg gtgatttgca ggttatgttt gtggtcaagg
    13561 ccaggaactt tattcagtac ctggatatcc acgccttcat caggttgcag acggatagtc
    13621 agtttattct gcggcagatc ctgccacgac tctttaaaca gattcagttc aggtgttttg
    13681 aaatagacca cgacttcaga acatttggtc ggcagacgtt taccagtacg caggtagaat
    13741 ggcacaccgg cccagcgcca gttatcaatg tcgacgcgga tcgccacgaa ggtttctgta
    13801 ttgctgcttt tgttcgcgcc ctcttcttcc agatatcccg gcactttttt gccctgggcg
    13861 aagcccgcag tatattgccc gcgtacggtt ttttcgcgta cgttggagcg gtcgatgcgg
    13921 cgcagggact tcagtacttt cactttttca tcgcggatgc tgtctgcgct caggtcagat
    13981 ggcggagaca tcgcaatcat gcagagaatt tgtagcaggt ggttctgaat catgtcacgc
    14041 atctgaccgg ctttatcaaa atagccccag cgcccttcga tccccacttc ttctgccacg
    14101 gtaatctcaa catgatcaat ggtgcgattg tcccagttat tcacaaacag ggagttagca
    14161 aaacgcagcg ccaacaggtt cagcaccgtt tctttaccaa gatagtggtc gatacggtaa
    14221 acctggcact cctcgaagta ttcgccaacc tgatcattga tttcctgcga ggtcgccagc
    14281 gacgtcccca gcggtttctc catgactacg cgtgccggtt tagcattcag ttttgcctcg
    14341 ccaagccctt tgcaaattgc gccaaaagtg ctgggcggca tggcaaagta gttaatagtg
    14401 atacgatttt tttgatccag catcgcgccg agacggttga atgcagcagt gtcattgaca
    14461 tcgagattac aaaaatccag acgtgcactc agggtgtccc ataaaccttc atcaatggtt
    14521 tctttcatga aagtttcgag cgcctcgcgg acaactttgg tatatgccgc tttatcccag
    14581 tcagcacgcc ctacgccgat aatccgggtg tccgggttga gctgaccgac tttttccagt
    14641 tgatacaggg aaggcagcaa tttacgacgc gcaaggtcgc ctttcgcgcc gaaaatgacc
    14701 aggtcacagg cctgggctgt ttgcgttacc gccatgtcat tctccttaag ttaactaacc
    14761 cggtacttaa gccagggtat acttgtaatt ttcttacggt gcactgtact gcttttacga
    14821 gcttgcgaaa actgtaaacg cttatccacc cgtgcgatta cgggaaaagc gcgcaaagcg
    14881 cggcaaaact gataaaaaaa tcatcgtttt ccattcggta aaaatctgac actgatcatg
    14941 ttatgaaaaa aaataacaac ttttttatct gcttttgtca ttaacacggc acacaggcgt
    15001 aatatctgac aaaactgcat ttcgatttct ttcagtgcgg aaatcgtcat tacccgtgag
    15061 tctctttaca tcatgaatat gctggaaaaa atccagtctc agctggaaca tttgagcaaa
    15121 tcagagcgca aagttgccga ggtcattctg gcttcgcccg ataacgcgat ccattcgagt
    15181 attgctgctc tggcgctgga agccaatgtt agcgaaccga cggtgaatcg tttctgtcgc
    15241 agcatggaca cgcgcggttt tcctgatttt aaacttcatc tggcacaaag tctggcgaat
    15301 ggcactccct atgttaatcg caacgtcaat gaagatgaca gcgttgaatc atacacgggg
    15361 aaaatatttg agtccgcaat ggcaacgctc gatcatgtcc gtcattcact ggataaatct
    15421 gccatcaacc gcgccgtcga cttgctcact caggcaaaaa aaatcgcctt tttcggatta
    15481 ggctcttctg ccgccgttgc ccacgatgcg atgaataagt tctttcgttt taatgttccg
    15541 gtggtgtact ccgatgatat cgtgctgcaa cgcatgagtt gtatgaattg tagcgacgga
    15601 gacgtggtgg tgctgatttc tcacacagga agaacaaaaa atctggtcga gctggcgcag
    15661 ctggcacgcg aaaacgacgc catggtgatt gccctcacct ctgcgggtac cccgctcgcc
    15721 cgggaagcaa cgctggcaat taccctcgac gtaccggaag atactgacat ttatatgccc
    15781 atggtttctc gacttgcaca gctgaccgtg atagatgtgc tggcgacagg atttactttg
    15841 cgacgcggtg caaaattcag agataacttg aagcgggtca aggaagcgct gaaggaatcg
    15901 cgttttgata agcagttact taatttaagt gacgatcgct aaaagcgact gtcactgtcc
    15961 taatcttata cgacatccga atgagattaa tttatcgcca tcgcggcgtt atttcattcg
    16021 gatttcatgt tcaagcaaca cctggttgtt tcagtcaacg gagtattaca tgtccaaaag
    16081 gcttcgcaga acaaaaatcg ttaccacgtt aggcccagca acagatcgcg ataataatct
    16141 tgaaaaagtt atcgcggcgg gtgccaacgt tgtacgtatg aacttttctc acggctcgcc
    16201 tgaagatcac aaaatgcgcg cggataaagt tcgtgagatt gccgcaaaac tggggcgtca
    16261 tgtggctatt ctgggtgacc tccaggggcc caaaatccgt gtatccacct ttaaagaagg
    16321 caaagttttc ctcaatattg gggataaatt cctgctcgac gccaacctgg gtaaaggtga
    16381 aggcgacaaa gaaaaagtcg gtatcgacta caaaggcctg cctactgacg tcgtgcctgg
    16441 tgacatcctg ctgctggacg atggtcgcgt ccagttaaaa gtactggaag ttcagggcat
    16501 gaaagtgttc accgaagtca ccgtcggtgg tcccctctcc aacaataaag gtatcaacaa
    16561 acttggcggc ggtttgtcgg ctgaagcgct gaccgaaaaa gacaaagcag acattaagac
    16621 tgcggcgttg attggcgtag attacctggc tgtctccttc ccacgctgtg gcgaagatct
    16681 gaactatgcc cgtcgcctgg cacgcgatgc aggatgtgat gcgaaaattg ttgccaaggt
    16741 tgaacgtgcg gaagccgttt gcagccagga tgcaatggat gacatcatcc tcgcctctga
    16801 cgtggtaatg gttgcacgtg gcgacctcgg tgtggaaatt ggcgacccgg aactggtcgg
    16861 cattcagaaa gcgttgatcc gtcgtgcgcg tcagctaaac cgagcggtaa tcacggcgac
    16921 ccagatgatg gagtcaatga ttactaaccc gatgccgacg cgtgcagaag tcatggacgt
    16981 agcaaacgcc gttctggatg gtactgacgc tgtgatgctg tctgcagaaa ctgccgctgg
    17041 gcagtatccg tcagaaactg ttgcagccat ggcgcgcgtt tgcctgggtg cggaaaaaat
    17101 cccgagcatc aacgtttcta aacaccgtct ggacgttcag ttcgacaatg tggaagaagc
    17161 tattgccatg tcggcaatgt acgcagctaa ccacctgaag ggcgttacgg cgatcattac
    17221 catgaccgaa tcgggtcgta ccgcgctgat gacctcccgt atcagctctg gtctgccaat
    17281 tttcgccatg tcgcgccatg aacgtacgct gaacctgact gctctctatc gtggcgttac
    17341 gccggtgccc tttgatagcg ctaatgacgg cgtagcagct gccagcgaag cggttaatct
    17401 gctgcgcgat aaaggttact tgatgtctgg tgacctggtg attgtcaccc aaggcgacgt
    17461 gatgagtacc gtgggttcta ctaataccac gcgtatttta acggtagagt aagtacgttg
    17521 ccggatgcgg cgaaaacgcc acatccggcc tatagttcaa tgatagttca acagatttcg
    17581 aatattctga agcaaacttg aacttatcat caggcgaagg cctctcctcg cgagaggctt
    17641 ttttatttga tgggataaag atctttgcgc ttatacggct ggatttcgcc cggtttgcga
    17701 gttttcagca attttagtat ccaggtgtat tgttctggtc gcggaccaac aaaaatctcg
    17761 acttcttcat tcatccgccg ctcaatcgta tgatcatccg cctctaacag atcatccatc
    17821 ggtgggcgca cctgaatcgt cagacgatgc gtcttgccat cataaatcgg aaacagcggt
    17881 acaacgcgcg cacggcatac tttcatcaaa cgaccaatcg cgggcaacgt cgctttatag
    17941 gtggcaaaga aatccacaaa ttcgctgtgc tctgggccat gatcctgatc gggtaaataa
    18001 tatccccagt acccctgacg taccgactgg atgaatggtt taataccgtc atttctcgca
    18061 tgcagacgac cgccaaagcg acgacgcacc gtgttccaga cataatcaaa aaccgggttg
    18121 ccctgattat ggaacatcgc tgccattttc tgcccttgcg aggccatcag catggcagga
    18181 atatcgacgg cccaaccgtg cggcaccaga aagataactt tctcgttatt acgccgcatc
    18241 tcttcgatga tctccagccc ttgccagcca acgcgcggct gaattttctc cggcccgcgt
    18301 attgccaact cagccatcat tgccatcgct tgcggcgcgg tggcaaacat ctcatcaaca
    18361 atcgcttcgc gttcagcttc actacgttct ggaaagcaga gcgacagatt gattaacgca
    18421 cgacggcgtg agctttttcc cagtcgtccg gcaaaacgtc ccagccgtgc cagaatggga
    18481 tcacggaact ttggcggcgt taaagcgata cccgccatcg ctgctacacc cagccatgct
    18541 ccccagtagc gcgggtggcg aaaggattta tcaaactcag gaatgtattc gctattattt
    18601 tttttcgttt ccatgctttt ccagtttcgg ataaggcaaa aatcaatctg gtgatagtgt
    18661 agcggcacaa cttgccccgc accaaataaa aaaagccggt actgactgcg taccggctgc
    18721 gaatggatgt taattaatca aaccgtagct gcggcacaat ctctttggcc tgcgccagga
    18781 attcgcgacg atcggagccg gtcagccctt cggtacgcgg cagttttgcc gtcagcgggt
    18841 ttacggcctg ctggtttatc catacttcat agtgcagatg cggcccagtt gaacgtccgg
    18901 tattaccgga aagcgcgata cggtcgccac gtttcacctt ctgtcccggt ttcaccagaa
    18961 tcttgcgcaa gtgcatataa cgcgtggtgt agctgcgacc atgacgaata gccacataat
    19021 aacctgctgc gccactgcgt ttggcaacca ccacttcacc gtcacccact gaaagcactg
    19081 gcgtaccttg tggcatggcg aaatcaacac ctctgtgtgg tgcaacgcga cctgtcaccg
    19141 gattagttcg acgcgggtta aagttagacg agatacggaa ctgtttcgcc gtcgggaatc
    19201 gcaagaatcc tttcgccaga ccagtaccgt tacgatcgta gaatttgcca tcctcagcgc
    19261 ggattgcgta ataatcttta ccttctgaac gcaaacgtac gcccagcagc tggctttgct
    19321 cacgtttacc atcaagcatt tctcgagaca ttaataccgc aaattcatcg ccttttttca
    19381 gtttgcggaa atccatttgc cactgcatgg ctttaatcac tgcgctcact tcggcgctgg
    19441 ttaaaccggc gtttctggcg ctggcaacaa agctccctcc gacggtacct ttcagcagat
    19501 tgttaaccca ctctccttgc tgcatttcgc tggtcatttt aaaaccgtta gcggcagtac
    19561 ggtcataggt tcgggtttca cgacgagaca cttcccaggt gaggcgctgc agttcgccgt
    19621 ccgcggttaa tgtccaggag agttgttgac cgattttcag gttacgcaat tctttgtcgg
    19681 cagcagcaag ttgggtgata tcacccatat caataccata ctgattgaga atgctgctta
    19741 gcgtatcgcc agtggaaaca acatattcat gcacgcccgc ttcaccggcg attttgtcat
    19801 ccagttcgtc ctggggaatg gcttcatctt cttgtgcagc ttgatcaatc ggctcactgg
    19861 cttcaggtaa gagcgaacga atttcgttct gttccagctc aatggttttg acaattggcg
    19921 tggcatcacg gtgataaaca tagggccgcc agacagcgac ggccagagta agaacggtga
    19981 gcgaccccaa cataacgcgg tgtggtcgcg gtaaattatt aaacgccagg gcgacagagc
    20041 gggctatctg ttgcacgtaa tcacttcctc attaatctcc tttcaggcag ctcgcatact
    20101 ggttggctaa ttgactcagg aattctgaat agcttgtttt acccagtttg atattcgtcc
    20161 ccaggggatc caacgttccc atacgaacgg atgtccctcg tgcgacgctc tcaacgaccg
    20221 ctggcctgaa ctgtggctca gcaaaaacgc aggttgcttt ttgctcaacc aactgtgttc
    20281 ttatttcatg taaacgctgc gcgccaggtt gaatctcagg gttaacggta aaatgaccaa
    20341 gcggtgtcag tccgaactgt ttttcgaaat agccgtaagc atcgtgaaaa acgaaataac
    20401 ctttcccctt gagcggcgcg agctcgttac caacctgcgt ttcggttgag gccaattgtg
    20461 cctcaaaatc cttcaggttg gcgtcaagtt tggctcgact ttgcggcata agttccacta
    20521 attttccatg gattgcaacc gctgtagccc gcgctatctc tggggaaagc caaagatgca
    20581 tgttgaaatc gccgtgatgg tgatcttcgt cacttttttc cgcgtggtcg tgatcatcat
    20641 catcgccgtg aatacttttc atcagtagcg gtttcacatc ttcaagctgc gcaatcgtta
    20701 cctgcttcgc ttctggtaat ttgcttaccg gtttttgcat aaacgcttcc atctccgggc
    20761 caacccaaac gactaagtcc gcgttctgta agcgttttac atccgatgga cgcagtgaat
    20821 aatcatgttc tgaagcgccg tcaggcagta aaacctccgt ttctgttaca ccatcagcaa
    20881 tggcagaagc gatgaaccca acgggtttaa gcgaagcgac aacggcggca tctgcggcct
    20941 gtgttgcacc tccccagaga gcggcggata atgctgcgaa aagaagcgtt tttttatgta
    21001 acataatgcg accaatcatc gtaatgaata tgagaagtgt gatattataa catttcatga
    21061 ctactgcaag actaaaatta acatgacaag tctggtttcc ctggaaaatg tctcggtttc
    21121 ttttggccaa cgccgcgtcc tctctgatgt gtcgctggaa cttaaacctg gaaaaatttt
    21181 gactttactt gggccaaatg gcgcaggtaa gtcgacactg gtacgggtag tgctcgggct
    21241 ggtaacaccc gatgaagggg ttatcaagcg caacggaaaa ctgcgcatcg gctatgtacc
    21301 gcagaagctg tatctcgaca ccacgttgcc actgaccgta aaccgttttt tacgcttacg
    21361 ccctggtaca cataaagaag atattttgcc tgcactgaaa cgtgtccagg ccgggcacct
    21421 gattaacgca ccgatgcaaa agctctctgg tggcgaaacg cagcgtgtac tattagcgcg
    21481 agcattgtta aattgcccgc aattattagt gctggatgaa cccactcaag gcgtggatgt
    21541 aaatggccaa gtggcgttat atgaccttat tgaccagttg cgtcgtgaac tggattgtgg
    21601 cgttttaatg gtttctcacg atctgcatct ggtgatggca aaaaccgatg aagtgctgtg
    21661 cctgaatcac cacatttgtt gttccggcac accggaagtt gtttccctgc atccggagtt
    21721 tatttcaatg tttggtcctc gtggtgctga acaactgggt atctatcgcc atcatcataa
    21781 tcatcgtcac gatttacagg gacgaattgt tttgcgtcgg ggaaatgatc gctcatgatt
    21841 gaattattat ttcccggttg gttagccggg atcatgctcg cctgtgccgc gggtccgctg
    21901 ggttcgtttg tagtctggcg tcgtatgtct tatttcggtg atacgctggc tcatgcctca
    21961 ttacttggtg tcgcgtttgg tttgttgctg gacgtgaatc cattctatgc ggtgattgcc
    22021 gttacgctgc tgctggcggg cggtctggta tggctggaga agcgtccaca gctggcgatc
    22081 gacacgttat tagggattat ggcgcacagt gccctgtcgc tgggcctagt ggtcgttagt
    22141 ctgatgtcta acattcgtgt tgatttgatg gcttacctgt tcggtgattt actggcagtg
    22201 acgccagaag atctcatctc tattgcgatt ggcgtggtca tcgtggtggc tattttgttc
    22261 tggcaatggc gcaatttgct gtcgatgacg attagcccgg atctggcgtt tgttgatggt
    22321 gtgaaattac agcaagtgaa attgttgttg atgctggtga cggcattgac gattggtgta
    22381 gcgatgaaat ttgtcggtgc gttgattatt acttcgttgc tgattattcc tgcagctact
    22441 gcgcgtcgct ttgcccgtac gccggaacag atggctggtg tcgctgtttt ggtggggatg
    22501 gtggcagtaa ctggcggttt aaccttttcc gcgttttacg atacgccggc gggtccgtcg
    22561 gtggtgctat gtgcggcact gttatttatt atcagtatga tgaaaaagca ggccagctaa
    22621 tctatcgctg aacacatttg tcggatgcgg cgcgagcgcc ttatccgacc tacggttcgc
    22681 tatctctggc tggcctgata agacgcgaac agtgtcgcat cagacacact gccagtgtcg
    22741 gatgcggcgc gagcgaccaa tccgacttac ggcatttctg gcggcgttat gccaaagtga
    22801 ttccacgccc gcgtcgtcgc catacgcccg cgcggtgtac gctgcaaaaa gccttgctga
    22861 atcaaataag gttccagtac atcttcaatg gtttcacgtt cttcgccaat ggctgccgcc
    22921 aggttatcca gacctaccgg cccaccaaag aacttatcga ttaccgccag caacaatttg
    22981 cggtccatat aatcgaaacc ttcagcatcg acattcaaca tatccagcgc ctgagcagcg
    23041 atatctgccg agatggtgcc atcgtgcttc acttcggcga aatcacgcac tcgacgcagc
    23101 agacggttgg caatacgtgg cgtaccgcga gcgcgacgag ctacttcctg cgcaccgtca
    23161 tcactcatct caagccccat aaagcgtgca ctgcgactga cgatatattg cagatccggc
    23221 acctgataaa actccagacg ttgcacaata ccaaaacggt cgcgcaacgg tgatgtcagc
    23281 gaacctgcgc gcgtggttgc gccaatcagg gtaaacggcg gcaaatcaat tttaatggag
    23341 cgtgccgccg gaccttcacc aatcatgata tccagttggt agtcttccat tgccgggtac
    23401 agcacttctt caacaactgg cgataaacgg tggatctcat caataaacag cacgtcatgc
    23461 ggttcaaggt tagtgagcat cgcagccaaa tcgcccgcct tttccagcac cggaccagaa
    23521 gtcgtgcgta aattaacgcc catttcattg gcgacaatgt tggcaagcgt agttttaccc
    23581 aaccccggag gaccaaaaat caacaaatga tcgagggcat cgccgcgcag tttcgctgct
    23641 ttgatgaaaa tctccatctg tgaacgaacc tgcggctgac caacatactc ttccagtaat
    23701 ttagggcgaa tggcgcgatc tgctacatct tccggcaaag tggtaccggc agaaatcaga
    23761 cggtctgctt caatcatcct ttacctcata acgcggcgcg tagggcttcg cgaattaatg
    23821 tttcactgct ggtgtcaggg cgagcgattt tgctcaccat gcggcttgct tcttgtggtt
    23881 tatagcctag cgccaccagc gcggcaactg cttcttgttc agcatcgtcg gtcgccgggc
    23941 tagcaggaga cgtgagtacc aggtcggcgg ctggcgtaaa gagatcgcca tgcaaacctt
    24001 taaatcggtc tttcatttca acaatcaagc gttcggcggt ttttttgcca atacccggca
    24061 gtttcaccag tgcccccact tcttcacgct caacggcatt aacgaactgc tgcgctgaca
    24121 ttccggagag gatcgccagg gccaacttcg ggccgacgcc gttggttttg atcaactctt
    24181 tgaacaacgt gcgttcttgt ttattgttaa aaccgtacag cagttgcgcg tcttcacgca
    24241 ccacaaagtg ggtgaaaacg atcgcttcct gacccgcttc agggagttca taaaaacagg
    24301 tcatcggcat atgcacttca tagcctacgc cgcccacttc aattaacacc agcgggggtt
    24361 gtttttcaat gatgatgcct ctgagtctgc ctatcacatg acgctcctgc gtaatgaatc
    24421 aaagataatg ctgtatgata aaaaaatgct ggatagatat ccagcgaagg atgaagaaaa
    24481 cttgcgaggt gtcccgatga tctgaaaaat ggcacagtat aatttattca acaggttata
    24541 ttggaagcaa atatttgaat attacatatt cagtgaagaa atgcgtaata aaaatataca
    24601 ttgcgactcc tgaaaaataa tttttttatg ctattacgta tatttgtatc tatttcaatg
    24661 gaatgacaac gtgaatatta attaccctgc tgaatatgaa attggagata tcgtctttac
    24721 atgtataggt gctgccttat ttggtcaaat atcagctgca tcaaattgct ggagtaatca
    24781 cgtcgggatc attatcggtc ataacggtga agacttcctg gttgcagaaa gccgggttcc
    24841 cctttcaacc atcaccacgc tatctcgttt tattaaacgt tcgtctaatc aacgctatgc
    24901 tataaagcga ttagacgctg ggctgacaga acaacaaaaa caacgaattg ttgaacaggt
    24961 tccctcccgg ctacgcaaac tttaccacac gggttttaaa tacgaatctt cgcgccagtt
    25021 ctgttcaaaa tttgtctttg atatttataa agaggcgcta tgtattccgg tgggtgaaat
    25081 agagacgttt ggacaactgt taaatagcaa tccgaatgca aaactcactt tctggaaatt
    25141 ctggttctta ggttctattc cgtgggagcg taaaaccgtc acgccagcca gtttgtggca
    25201 tcatccgggt ttggtgttga ttcacgcgga gggagttgaa acgcctcagc cggaactgac
    25261 cgaggcggta taacttaacg cagtcgccct ctcgtcaggt tcagccgcga ttcgctcatc
    25321 tgcatcgcat tctgactaac gtggcagtgg gtgatggcaa tcgccagcgc atcggcggca
    25381 tccgcctgtg gattagcggg cagtttcagc aaggtgcgga ccatatgctg cacctggctt
    25441 ttttcggcac tacccatacc taccaccgtt tgctttacct gacgtgccgc gtattcaaat
    25501 accggcaatt cctgattcac cgccgcaaca atcgccacgc cgcgcgcctg gcccagtttc
    25561 agggctgagt cagcgttctt cgccataaag acttgttcaa tggcgaaata atcaggctgg
    25621 aactgggtga tgatttccgt cacgcccgca tagatgagct tcagacgaga cggtaaatca
    25681 tccactttgg tgcgtatgca tccgctaccc aggtaggaca gttgcctacc tacctggcgg
    25741 atgacgccgt agccggtcac gcgcgaaccc gggtcaatgc cgagaataat agccatcacg
    25801 cgtctccgtt ttgctgttta gcaggcatca tcagagagtc gctgcaacct catcagagat
    25861 ttcaccgtta tggtaaactt cctgcacgtc gtcgcaatct tccagcatat cgatcagacg
    25921 catcagtttc ggcgcggttt ctgcatccat atcagctttg gtggacggga tcatggaaac
    25981 ttccgcgctg tctgctttca gacctgccgc ttccagagcg tcgcgcactt tacccatttc
    26041 ttcccatgca gtgtagacat caatcgcgcc gtcatcatat gtcacaacgt cttcagcacc
    26101 tgcttccaga gctgcttcca tgatggtgtc ttcgtcgcct ttctcgaagg agatcacgcc
    26161 ttttttgctg aacagataag ctacggaacc gtcagtgccg aggttaccgc cacatttgct
    26221 gaatgcgtga cgcacttcag caacggtacg gttgcggttg tcagacagac attcaatcat
    26281 gattgccgtg ccgccaggac cgtaaccttc gtagatgatg gtttccatgt ttgcatcatc
    26341 atcaccgccc acaccacgtg caattgcgcg gttcagtgtg tcacgggtca tgttgttaga
    26401 cagtgcttta tccaccgccg cacgcagacg cgggttagcg tccggatcgc caccgcccag
    26461 cttagccgcg gttaccagct cacgaatgat tttagtgaag attttaccgc gcttagcatc
    26521 ctgcgcagct ttacgatgtc tggtgttggc ccatttacta tgacctgcca taaaaatatc
    26581 tccagatagc cctgcctgtt caggcagcgt taattacaaa ctgttcaatc gcctgccggt
    26641 tgctccagga cttagtgagc gccgccgcag caggcgcatc aagccacttg taagccagat
    26701 gttcagtgaa aacgatctgc cgctcgtgcg gaagcgcaag acagaaccat gattccgtat
    26761 tacgcgtcac gcccggcgca tagcgatgac gtaaatgtga aaaaatttca aactctaccg
    26821 tgcgctgaca gtcaattaag gtcagttgtt cagcgacaac atcaatggta acctcttcct
    26881 ttacttcgcg catggcagct tgcggcgcgg tttcaccctc ttccacgctg ccggttaccg
    26941 actgccagaa atcgggatcg tcacgccgct gcaacatcag cacccgtttc gtatcttgtg
    27001 catagatgac cactaagatc gaaacgggac gcttatacac tttatccttc acgctgcctc
    27061 ttcgttgact gccttcgctc atcccattca catagttatc tatgctcatg ggagttcact
    27121 cagttgccgc cttgatgcaa cgcgaatgat ttcgtgtatt tgagtcatat cagttattct
    27181 cagccttctt cacaacctga atgctcagct cagccagtgc agtcgggtta gcaaagctcg
    27241 gtgcttcagt catcagacac gctgccgccg tggttttcgg gaaggcgata acgtcacgga
    27301 tattgtcggt gccagtcagc agcatggtca gacggtcaag accgaatgcc agacctgcgt
    27361 gcggcggagt accgtatttc agggcgtcga gcaggaagcc gaatttctcg cgctgttcct
    27421 cttcgttgat acccagaata ccaaacaccg tctgctgcat atcaccatta tggatacgta
    27481 ctgaaccacc gcccacttcg taaccattga tgaccatatc gtaagcgttc gccaccgcat
    27541 tttccggtgc agctttcagt tctgcagcag tcatatcttt cggtgaggtg aacggatggt
    27601 gcattgccgt caggccgcct tcaccgtcgt cttcaaacat cgggaagtcg ataacccaca
    27661 gcggtgccca tttgctttcg tcggtcagac caaggtcttt acccactttc aggagcagtg
    27721 cgcccatccc gtcggcaaca attttcttgt tgtcggcacc gaagaaaatc atatcgccat
    27781 cttgcgcggc agtacgatcc aggatggctt cgatgatttc tgcattaagg aacttcgcta
    27841 ccgggctgtt gataccttcc agacctttcg cgcgttcgtt aactttgatg taagccagac
    27901 ctttcgcgcc gtagatttta acgaagttac cgtattcgtc gatctgctta cgggtcagcg
    27961 atgcgccacc cggaacacgt agagcggcaa cacggccttt cggatcgttc gctggccccg
    28021 caaatactgc aaactcaaca gatttcagca gatcggcaac gtcggtcagt tccatcgggt
    28081 tacgcagatc cggtttatca gaaccataac ggcgttctgc ttccgcaaag gtcattaccg
    28141 ggaaatcgcc cagatccacg cccttcactt ccagccacaa atgacgcacc agcgcttcca
    28201 tcacttcacg cacttgcggc gcggtcatga aagaagtttc cacatcgatc tgagtaaatt
    28261 caggctgacg atcagcgcgc aggtcttcgt cacggaagca tttaacgatc tgatagtaac
    28321 ggtcaaaacc ggacatcatc agcagctgtt tgaacaactg tggggattgc ggcagtgcgt
    28381 agaatttacc tttgtgcaca cgagaaggca ccaggtagtc acgcgcgcct tccggcgtgg
    28441 ctttggtcag catcggagtt tcgatgtcga ggaagccgtg gtcatccata aaacggcgca
    28501 ccaggctggt gattttagcg cgggttttca ggcgttgcgc catttccggg cgacgcaggt
    28561 cgaggtagcg gtatttcaga cgcgcttctt cggtgttgac gtggttagag tcaagcggca
    28621 gaacatctgc gcggttgatg atagtcagcg aggacgccag cacttcaatt tcgccggtcg
    28681 ccatatcgcg gttaatattt ttttcgtcac gcgcacgtac ggtgcccgta acctgaatgc
    28741 agaactcatt acgcagttca gaggccagct ttaacgcgtc cgcacgatcc ggatcgaaaa
    28801 atacctgcac gataccttcg cggtcgcgca tatcgatgaa gatcaggcta ccaagatcac
    28861 gacgacggtt gacccaacca cacagagtca cctgctgccc cacgtgggac aaacggagct
    28921 gtccacaata ttctgtacgc atgagatatc ccttaactta gctgccggcg gatgccccct
    28981 gctgcgcagg tgaccaagtc gcagcgttag ctgtatgtca caactgaatg aaaaaaggcg
    29041 gctattatac tggaaattct gccgcaccgt aagagcctgg cccgcgctgg aacgcctcgt
    29101 taccacttta tatcgggcct gaaatcagac tctacgccag tttgctataa aggtgttgcc
    29161 cgaactcata aaaattaaca aaatttgtcg ttccgccatc ggctaatcgc attaaggtgt
    29221 gaggcacgat tttgtttttt caggagtcag catgcttgaa cttaatgcta aaaccactgc
    29281 gttggtggtg atcgatttac aagaaggcat cttacctttt gccggtggtc cacatactgc
    29341 cgatgaggta gttaatcgcg ccgggaagct ggcggcgaaa tttcgcgcca gcggtcagcc
    29401 cgtgtttctg gtgcgagttg gctggtctgc cgattacgcc gaagcattaa aacagccggt
    29461 tgat
//
LOCUS       DAJTRD010000045        29043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTRD010000045 DAJTRD010000000
VERSION     DAJTRD010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.045"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO315_002190"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO315_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5891092.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO315_002191"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO315_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5891093.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO315_002192"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO315_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5891094.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO315_002193"
     CDS             4843..5082
                     /locus_tag="OO315_002193"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891095.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO315_002194"
     CDS             5092..5262
                     /locus_tag="OO315_002194"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5891096.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO315_002195"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO315_002195"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5891097.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO315_002196"
     CDS             6724..6990
                     /locus_tag="OO315_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5891098.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO315_002197"
     CDS             7084..7773
                     /locus_tag="OO315_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5891099.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO315_002198"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO315_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5891100.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO315_002199"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO315_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5891101.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO315_002200"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO315_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5891102.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO315_002201"
     CDS             complement(10475..12193)
                     /locus_tag="OO315_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5891103.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO315_002202"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO315_002202"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5891104.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO315_002203"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO315_002203"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5891105.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO315_002204"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO315_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5891106.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO315_002205"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO315_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5891107.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO315_002206"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO315_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="OO315_002207"
     CDS             18432..18746
                     /locus_tag="OO315_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5891108.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO315_002208"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO315_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5891109.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO315_002209"
     CDS             20570..21862
                     /locus_tag="OO315_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5891110.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO315_002210"
     CDS             complement(22016..23665)
                     /locus_tag="OO315_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5891111.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO315_002211"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO315_002211"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5891112.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO315_002212"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO315_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5891113.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OO315_002213"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OO315_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5891114.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO315_002214"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO315_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="OO315_002215"
     CDS             28753..29034
                     /locus_tag="OO315_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5891115.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7283 a         7775 c         7353 g         6632 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcgctgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcgatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtaaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacactaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaa
//
LOCUS       DAJTRD010000046        28915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTRD010000046 DAJTRD010000000
VERSION     DAJTRD010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.046"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO315_002216"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO315_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5891116.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO315_002217"
     CDS             complement(2138..2584)
                     /locus_tag="OO315_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5891117.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO315_002218"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO315_002218"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5891118.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO315_002219"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO315_002219"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5891119.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO315_002220"
     CDS             complement(5347..6009)
                     /locus_tag="OO315_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5891120.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO315_002221"
     CDS             6182..6595
                     /locus_tag="OO315_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5891121.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO315_002222"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO315_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5891122.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO315_002223"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO315_002223"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5891123.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO315_002224"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO315_002224"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5891124.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO315_002225"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO315_002225"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5891125.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO315_002226"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO315_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5891126.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO315_002227"
     tRNA            complement(9861..9948)
                     /locus_tag="OO315_002227"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO315_002228"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO315_002228"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5891127.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO315_002229"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO315_002229"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5891128.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO315_002230"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO315_002230"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5891129.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO315_002231"
     CDS             14005..14592
                     /locus_tag="OO315_002231"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5891130.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO315_002232"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO315_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5891131.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO315_002233"
     CDS             14984..15841
                     /locus_tag="OO315_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5891132.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO315_002234"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO315_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5891133.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO315_002235"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO315_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5891134.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO315_002236"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO315_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5891135.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO315_002237"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO315_002237"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5891136.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO315_002238"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO315_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO315_002239"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO315_002239"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5891137.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="OO315_002240"
     CDS             complement(22884..24023)
                     /locus_tag="OO315_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5891138.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="OO315_002241"
     CDS             complement(24069..26165)
                     /locus_tag="OO315_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5891139.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="OO315_002242"
     CDS             complement(26165..26911)
                     /locus_tag="OO315_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5891140.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="OO315_002243"
     CDS             complement(26908..27552)
                     /locus_tag="OO315_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5891141.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="OO315_002244"
     CDS             complement(27659..27964)
                     /locus_tag="OO315_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891142.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO315_002245"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO315_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5891143.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7004 a         7321 c         7600 g         6990 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgcctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagacaaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agttttaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataaa
//
LOCUS       DAJTRD010000047        28674 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTRD010000047 DAJTRD010000000
VERSION     DAJTRD010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28674
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.047"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..653
                     /locus_tag="OO315_002246"
                     /pseudo
     CDS             1..653
                     /locus_tag="OO315_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            650..1036
                     /locus_tag="OO315_002247"
     CDS             650..1036
                     /locus_tag="OO315_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891144.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1061..3274
                     /gene="tssI"
                     /locus_tag="OO315_002248"
     CDS             1061..3274
                     /gene="tssI"
                     /locus_tag="OO315_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR5891145.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTPDVSE"
     gene            3297..3742
                     /locus_tag="OO315_002249"
                     /pseudo
     CDS             3297..3742
                     /locus_tag="OO315_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3764..4171
                     /locus_tag="OO315_002250"
     CDS             3764..4171
                     /locus_tag="OO315_002250"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR5891146.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4168..6456
                     /locus_tag="OO315_002251"
     CDS             4168..6456
                     /locus_tag="OO315_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5891147.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6453..7442
                     /locus_tag="OO315_002252"
     CDS             6453..7442
                     /locus_tag="OO315_002252"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891148.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7542..7745
                     /locus_tag="OO315_002253"
     CDS             7542..7745
                     /locus_tag="OO315_002253"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891149.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7763..8083
                     /locus_tag="OO315_002254"
     CDS             7763..8083
                     /locus_tag="OO315_002254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891150.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8149..8381)
                     /locus_tag="OO315_002255"
                     /pseudo
     CDS             complement(8149..8381)
                     /locus_tag="OO315_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8491..9261)
                     /gene="yafV"
                     /locus_tag="OO315_002256"
     CDS             complement(8491..9261)
                     /gene="yafV"
                     /locus_tag="OO315_002256"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5891151.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9442..9888
                     /gene="ivy"
                     /locus_tag="OO315_002257"
     CDS             9442..9888
                     /gene="ivy"
                     /locus_tag="OO315_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5891152.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9931..12375)
                     /gene="fadE"
                     /locus_tag="OO315_002258"
     CDS             complement(9931..12375)
                     /gene="fadE"
                     /locus_tag="OO315_002258"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5891153.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12615..13193
                     /gene="lpcA"
                     /locus_tag="OO315_002259"
     CDS             12615..13193
                     /gene="lpcA"
                     /locus_tag="OO315_002259"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5891154.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13399..14166
                     /locus_tag="OO315_002260"
     CDS             13399..14166
                     /locus_tag="OO315_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5891155.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14137..14877)
                     /locus_tag="OO315_002261"
     CDS             complement(14137..14877)
                     /locus_tag="OO315_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5891156.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15261..15782
                     /locus_tag="OO315_002262"
                     /pseudo
     CDS             15261..15782
                     /locus_tag="OO315_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15958..16455
                     /gene="rayT"
                     /locus_tag="OO315_002263"
     CDS             15958..16455
                     /gene="rayT"
                     /locus_tag="OO315_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5891157.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16672..18384)
                     /locus_tag="OO315_002264"
                     /pseudo
     CDS             complement(16672..18384)
                     /locus_tag="OO315_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18356..19141
                     /gene="lafU"
                     /locus_tag="OO315_002265"
     CDS             18356..19141
                     /gene="lafU"
                     /locus_tag="OO315_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5891158.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19212..20267
                     /gene="dinB"
                     /locus_tag="OO315_002266"
     CDS             19212..20267
                     /gene="dinB"
                     /locus_tag="OO315_002266"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5891159.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20319..20612
                     /gene="yafN"
                     /locus_tag="OO315_002267"
     CDS             20319..20612
                     /gene="yafN"
                     /locus_tag="OO315_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5891160.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20615..21013
                     /gene="yafO"
                     /locus_tag="OO315_002268"
     CDS             20615..21013
                     /gene="yafO"
                     /locus_tag="OO315_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5891161.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21023..21475
                     /locus_tag="OO315_002269"
     CDS             21023..21475
                     /locus_tag="OO315_002269"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5891162.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21721..22764
                     /locus_tag="OO315_002270"
     CDS             21721..22764
                     /locus_tag="OO315_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5891163.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22826..23440
                     /locus_tag="OO315_002271"
                     /pseudo
     CDS             22826..23440
                     /locus_tag="OO315_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23497..24954)
                     /gene="pepD"
                     /locus_tag="OO315_002272"
     CDS             complement(23497..24954)
                     /gene="pepD"
                     /locus_tag="OO315_002272"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5891164.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25216..25674
                     /gene="gpt"
                     /locus_tag="OO315_002273"
     CDS             25216..25674
                     /gene="gpt"
                     /locus_tag="OO315_002273"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5891165.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25766..27010
                     /gene="frsA"
                     /locus_tag="OO315_002274"
     CDS             25766..27010
                     /gene="frsA"
                     /locus_tag="OO315_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5891166.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27068..27469
                     /gene="crl"
                     /locus_tag="OO315_002275"
     CDS             27068..27469
                     /gene="crl"
                     /locus_tag="OO315_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5891167.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27508..28563)
                     /gene="phoE"
                     /locus_tag="OO315_002276"
     CDS             complement(27508..28563)
                     /gene="phoE"
                     /locus_tag="OO315_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5891168.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7086 a         7124 c         7606 g         6858 t
ORIGIN      
        1 aaaaaacaaa tattgatggc aatatcatta tacataagga tggtaggagg acgtatttat
       61 taaatcaaaa tactggttcg attacggctg tgataagatg gaagtatgag tagtataagg
      121 ataataccgt tacagatgac tggactatat gggaaaaagc tatttttatg cgagcgatat
      181 atttatctgt tcagcaggca tggaatggga aaataactta ttctgtttcc ggtgaatctg
      241 agttcgctaa aaaatttcag ggtaaagctt tgccttttga tgtaagaata atttctgcat
      301 ctcagaatga agactggttg gttattgcaa caaaggtttt gcctggtgct gatttaagaa
      361 catatgttga ttttaaaaac agtacggttc atgttgattc agcgggcctg gaaaaagtag
      421 cgaaatgtat taactgtaat aatacgttgc aagtaaatat acctcatgaa gccggacatg
      481 tacttggtta cttagatgat gattatgatt cttcgtctcc gtatgttgga gatattagtg
      541 ggttgatgaa tgtgggaatg gagctttggg aacgatacct taaaaacgca actataacgt
      601 tgaatatcat aatgcctgaa actaaattta ctttattgaa tgtaaccaaa tgaaattttt
      661 ctgtattata gtgattttcc tttcaataaa ttacatagct atagtgaatg ttttcaagtc
      721 ttctgcagtt gctcaatcca ccaaatatta tgaagaaaat gatatacatg atggaagtaa
      781 tgacatcttt tttatgaaga aatatgggcc gcctgctcat gagttcgtga ggccacgata
      841 taaatttgtt ggtgagtatt atatcggctt gaatcaaact tatcgcataa cagatcctga
      901 atttcaagat gttcctatta aggaaatgtt ctggaatttg cagggggatt tgaatctgac
      961 atgttggttt cattataaag acgggcaatg gagagtgatt acttatattt tttggccacc
     1021 aggggctaga ttttaatccg gtattaaagg agcaaatatc atgagttttg tatctacggg
     1081 taataaacca gttggaaacg gtggacccgt catcaccaca ccaccaatag ctggcgaaag
     1141 tggcggtatg agcacaggtt cagctgtaac cgatgtatct ggtgccgccg aggagatggc
     1201 agaacaggct gcggcggacc tgtttggtgc gctgccggaa ccgtcaggtc tggttaaagc
     1261 cgcagtagct gctgcgcagg ctgccgctgc agcagcaggg atctcggata tggctggtgc
     1321 agttcaggat gcagcggcgt cgcttgcagc cggtgctccg ggggcgcata acgtcacagt
     1381 aagtggtagt gcggtgccgc cgcagatgct tttgtttgca ggcatgaatg gttcggaagg
     1441 gcttgggaat ctgttttctt acacagtaca gcttaaaacg ctggacgccc tgaacctggg
     1501 gtacgtttcc cccgctgcta acctgccgct tcagctcatg gtgggcaaag acctgtgcgt
     1561 cagtatcgaa ctggacggcg gcggaaaacg gtatatcagc gggctggtga cggcggcgag
     1621 ggtggtcggc catgagagcc gttcggtaac gtatgaatta cgcattgagc catggctgaa
     1681 gctgctgacc cataccagcg actacaaagc gttccagaac aaaaccgtgg tggatattct
     1741 cgatgaagta ctggatgaat attcctttcc ggtggaaaag cggctggtgg aaaattatcc
     1801 accccgcgcc tggcaggtgc agtatggtga aaccgacttc gatttcatcc agcgcctgat
     1861 gcaggaatgg gggatttact ggtggtttga gcacagcgag aacagccaca cgctggtgct
     1921 ggtggatgcc atcaatggtc ataaagcgtg ccctgactcg ccgctggtgg agtggcacca
     1981 ggaagggctg aaactggata aggaatttat ccacaccatt acggccagtg agaggctgcg
     2041 tactggaaag tgggtgatgg acgattttga ttttatgaag cctcgttcgc tgcttaaaag
     2101 taccgtggcc agtccgcgtg ataccggtca tgctgaatac gagcattacg aatggcctgg
     2161 tgattacttc actacgggcg aaggcgagat gctgacgcgc attcgtatgg aagcgcagcg
     2221 cagcccaggc agccgggcac atggggccgg tcatatccgt accctcatga ccgggtacac
     2281 ctttacgttg atgaatcacc cgacggcaga aattaatcag gaatatctgc tggtacagac
     2341 cactctgttt ttacgggata atgcgcagca cagcggtcag gatcagcatt ttacctatgt
     2401 caccacgttt gaactgcacc ccaccagcga agtttaccgc ccacaacgaa ccctgagcaa
     2461 accccacacc aaaggcccgc agagcgccat cgtcaccggc ccggcggggc aggaaatctg
     2521 gacggataaa tatggccggg tgaaggtgca gtttggctgg gaccgttacg gaaagaatga
     2581 cgaaaacagt tcctgctgga tccgcgtcag ctatccgtgg gcgggtaaag gattcggcat
     2641 gatccagatc ccgcgtattg gccaggaagt actggtggat ttcaaaaacg gcgatccgga
     2701 tttgcccatc atcgtggggc gcacctataa ccaggacacc atgccgccgt gggggctgcc
     2761 gggcatggcg tcgcagagtg ggatattcag ccactcattg cagggagggc cgactaacgg
     2821 aaatatgctg cgcttcgatg ataaaacggg tgcggaagag gtgaagttcc atgcggaaaa
     2881 agacctcaat accacggtaa aaaacaatga aacccacacg gtcatggtcg atcgcataaa
     2941 aaccattgtc aaaaatgaaa ccatcagcgt gggagaagac cgtaatgcca ccatcacaaa
     3001 aaatgatggt ctttccgtca aactggctca aaccatcaat gtcggaacga catatcgtct
     3061 ggatgttggc gatcaattta cccttcgttg cggcaatgca gcgcttgtat tacacaagga
     3121 tggctcgatt gagttttgcg gaaaacaact gttactgcac accagcgatg ttatgcaact
     3181 cattggcagt ggcattgata tgaatccgga tggcggcaca gccatgactg cggatgatat
     3241 tgccccgtta cctacccccg acgtttctga ataaaaaata taaaaggaat ttttctatga
     3301 ccagccttta ccgtattcag gagggctgct ttgccctgcc tgaaactttt cttgatcgca
     3361 ccgtcaatat cttcgtccca tcgggcaatg aaagggcaac tcccagtctg aatattttcc
     3421 gcgatacgct caggccagat gaaaatctga ccacctatat tgaccgccag atagccttga
     3481 tgaaaaaaaa aacctaggcc agcaccgggt actttcgcgg ggaacggcac tggcgggaac
     3541 tggcgaagct gccatgacag gtgaacagat tctcgcaact catcgttctg gaaaaacaga
     3601 agtgtaccag cgtcaggccg ggtttatcac cgggccagca aaggtactaa tgttgacgct
     3661 gaccactcag cgtccttttg atgatcacac cgaccagcta tggactgcat ggttgactag
     3721 ttttcagcct gctaaaagct aaaaaaaaca gggaggtttt gtcatgtatg aagccgcacg
     3781 ggtggatgac cccatctacc acaccagcgc ccttgccggg tttttgatag gggccatcat
     3841 aggtatcgcc attattgccg tcgccagctt tgcttttttt acctgcggct ttttggccgg
     3901 gctgattttg agttttctgg ctgatcagat agcttccgga gtcctgcaac tgggtgaggc
     3961 tattgggcgc tcgatacatt ccacagtggg aaaaattctg accggttctg ataatgtcag
     4021 caccaacagt cgcccggccg cccgggccgt actgagtacg gtgatatgcg aagaccattc
     4081 cccggaaatc cgcattgccc aaggttccgg caacatctac atcaacagcc agctgcccgt
     4141 aaggatgacc acactgagtg tgatgcagtg attgaggacg gctcccccaa tgtctttctc
     4201 ggcggcggta cgcagacggt actggaaatc agcccggaaa tccctgactg gctgcgtcag
     4261 gtggtggatg tgctgtatgt ggtcgccgga atgcttggcg ggctggctgg cgcctggcgt
     4321 caggcggcaa agatggggtc gaaatttggt acaaaatgtg cggcaaaatt tattggtggc
     4381 gaaatggtcg ggatggccat ctctgacaca gtcatgggat tgttcagcaa tccggtagat
     4441 gtcaccactg ggcaaaaaat tttgctgccg gagacggatt ttaccctgcc aggacgcctg
     4501 cctgtcacct gttcgcgctt ttacgccagc cacctggaaa ctgaaggatt gctgggcaga
     4561 ggatggcggc tgaactggga gataaacctg cgtgaggatg aaacatatat caccttcatc
     4621 ggtgtacagg gtcgggaact gagttatccg aaagagatgc tgataccggg ccaccagata
     4681 ttcgacccgg aagagcagtt ttacctgagc cgtctgcatg acggatgcta tgtgctgcat
     4741 tacaccgact gcagctatta cgtgtttgat gaatttgatg accacggcgt cgcgccgtta
     4801 ctttttatgg agacgccgta ccggcagcgt attgctttcg ggcgtgagaa cggcaggctg
     4861 gtgcgggtgg cctccagcag cggacaccat ctgttattgc accgcacgat gacgcaagca
     4921 ggggaacgcc tgtcgcatat tgaacttttg aagggcgggc gtcccggtaa cctggtggaa
     4981 tatcgttacg acgataacgg gcaactgact ggcgtggtga accgggctgg tgtgacggtg
     5041 cgccagtttg cctatgaaaa tggcctgatg acagagcatc gcaacgcgac gggctttacc
     5101 tgtacctacc actgggaaga gattgaaggt ttcccgcgcg tggtggaaca cacgaccagc
     5161 gacggcgagg attaccggtt ccactatgat tttgctggcg ggcagacggt ggtaaccggc
     5221 aggccggagc agaagtggca gtggtggttt gatgaagaga cgtatgtgac tgcgcaccgg
     5281 acgccgggcg gcggaatgta ccgcttcacg tacaacgaaa accattttcc ggtagcggtg
     5341 gaactccccg gcgaacgcag ggtaacgctg gaatacgaca ccctgagccg ggtggtgaaa
     5401 gagacggacc cggcaggccg ggtgacacag acgcagtgga acggcagttt tgctgaaatc
     5461 acgcgccggg cgctggatga cgaccacgta tggaaggcgg attacaacga acacggccag
     5521 gtgatacggg agacggaccc ggaagggcgc gtcacgcggt acgggtatga tgatcagggg
     5581 ctgccggaga cggtctgcca ccccggtaaa cagcaggacc gttacacctg gaacgcgctg
     5641 gggttactga gcagccaccg tcgcatcacg ggcagtgtgc agtcctggca gtatacgcag
     5701 cgcgggatgc tggcgcgcca tacggatgaa gagaaacggg agacgcgctg gcagtacacc
     5761 ccggaagggc tggttgccag tctgagcaac ggcaacgggg ctcagtaccg gttcagctac
     5821 gacggcgacg gacggctgac gggagaacag cgcccggacg gcctgataag gatgtttgcg
     5881 ctgaatgcgg acgggtttcc ggtcatcatc ccgacgcagg gcacagaggg cggggtgcgc
     5941 aatgaacagc aggagcgtga tgcgctgggc cgcctgctgc gcagtgacac gcagcacagt
     6001 acacggacct tcagctacaa ccgtcttgac cagataacgg aagtgacgct cacccccacg
     6061 gaagaagggg aacggctgca ccacatgcag gcggacacgg tgcgttttgc gtatgaccgc
     6121 agcgggtggc tgacggcaga gcacagcgtg cacggcagta tcaaataccg gcgcgatgcg
     6181 ctgggcaatc ccaccgatat cacgttgccg gacgggcagc atctcagtca tctttactac
     6241 ggcagcgggc atctgctcca gacggcactg gacggtatca cggtcagtga atatgagcgt
     6301 gacagcctgc accgtcaggt gatacgcacg caggggaaac tggcgacgtt cagcggctac
     6361 aacgccgaca accggttaag ctggcagcgc agcctgccag gtggcagcca gaatccacag
     6421 caggcggtac tcccccggcg cagaacgaca gcgtgacggc gcgggactat atctggaacg
     6481 cagacggtga agtcggcggc atcaatgaca aactgcgcgg ttgcctggtg ttcagctatg
     6541 accgcagcgg ctggctgacc agccgcacgg ggcagatgta tgaccatgac cattattatt
     6601 acgacaaagc gggcaacctg ctgacggatg agtatcaggg agcggtgatg gataaccgcc
     6661 tgccgggtta cgggcgtgac cgctaccgct ataacgaatg gggcgaactg acagagcgca
     6721 gggaccagca gctggagtgg aacgcgcagg gccagttaac gcgggtcatc agcagcaaca
     6781 gcgagacgcg gtatcagtac gatgcgctgg gcaggcgcat cagcaaggcg accagcaatc
     6841 tccataccga ccggggagaa cgaagccgga caacgtttgt gtgggaaggg ttccggctgt
     6901 tacaggagac aacgtggcag ggaaagcgga catacctgta cgatgcggaa cagccgtaca
     6961 cgccggtggc tgccatcacg gggcgcggag agagccagaa aatctggtat taccacacgg
     7021 acctgacggg cacggtacac gaggtaaccg cgccggacgg gacgctggtg tgggcgggct
     7081 accaggcggg atttggtgaa aaccggggcg acatcagcaa cagcggggcg tactttgagc
     7141 agccgcagcg gctgccgggg cagtactttg acgaagaaac agggctgcat tacaatctgt
     7201 tcaggtatta tgcgccggag tgcgggaggt tcgtaagcca ggacccgata ggattaaacg
     7261 gtgggttgaa cttatatgct tatgcgccta atccgttagg ttggatcgat ccgctaggac
     7321 tgaattcttt aggaatagat tcaaataata tatttagagg agatggtaac tataaaggtg
     7381 gcggaattgg acagccaaag agtggcacaa tatcagcaca ggacattatt gatcatctct
     7441 aagaaaaagg tgatgggcgt ttaacatctt ttagtactaa aaaaacatct agttccaact
     7501 ctcgtggtgc agattttttc tcaaaaaacc atgtcttcaa agtggcagtt tctgatctcg
     7561 atgatttagt taaaaataat cagatcactg ttttaacaca agaaaaagta aaagcaatat
     7621 ttggtttaga atcaagcaag aaaattagaa aagaacttgg taatattctt cagaatatgg
     7681 ctaggaataa tgaggtgtta gttcatgggc aaattcttgg aaataaagtt agccgatgta
     7741 attagttagt ggagaggaag ttgtggacga aattagaata agaattgctg gtaatacggc
     7801 tgcgccttgt ttccatgtgc taaaaaataa gggatataaa gtaacaatgg aaactcatgc
     7861 attatctgac gattgtttag attgtgtata tgattggtat gcggaaaaaa atggacaatt
     7921 ttttagtgca acgagtccag aagagttact tggattagtt gcaatgtggg aagttcgtgg
     7981 taatgactgg agagctaatt tgaatgagat aaatacttat caaaaagcca tagaagaatc
     8041 cccgtttttt gataaaaacg gcaatgaaat agagtctgac taaattttat tgatatagca
     8101 acgataataa ccggaaattt cttccagatc tttccggctt tttattattt aactttcgtt
     8161 cagcacacta aacatcccgt ctcgcatccc cttaactggc tgtttaacct gataaagttc
     8221 cctgcgcagt tcctgtactt cgttgttatc ccaagcagga tcaaacatcc ctccgagatc
     8281 ttcacggtaa cgcccgtgcc gatctcaaat cccgcttttc tcagccattt ggcgctgatt
     8341 ttgatcaccg gcgtgtcgct gcggttgggc agataaccca cggtacagcg acgctgtgtt
     8401 ttggaaatgg tgacttctgg cgtaatattg tgtttaagtt aggggctttc gtaatctagc
     8461 ccagcccccg gccctaaaca caacccccac tcaccacaac ctaaactcat ccgcatcccg
     8521 ccatgccgga aacttttctc tatattcccg caatgccacc atcgacagct ccgcatcaat
     8581 acgcgttgcc ttatgcgcgt cggcggtagc gataatctcg ccttgcggat taatcacccg
     8641 gctgtcaccg cgataatggc agccgttgcc atcgctgccg acgcgattgc atcccgccac
     8701 atacgcctga ttctcaatcg cgcgggccgt cagcaatgcc tgccagtgca gagagcgcgg
     8761 agctggccag ttggcgacgt acagggcgag gtcataatcg ttgagattgc gcgaccacac
     8821 tggaaaacgt aagtcgtagc acaccagcgg caaaatacgc cagccgcgcc attccacaat
     8881 cactcgcgca ttgcccgctt tataatgcag atgctcatct gccatgcgga acagatgacg
     8941 cttatcataa aaatgtaccg tgccgcccgg ctcaaccagc aaaaagcggt taaccgaacc
     9001 agactccgtt tgtaatgcaa cactgcctgc aatcagcgca ttgcactgct gcgccttagc
     9061 tgtcatccag ttcactacgt catcttgtgc aagcgacgaa gctgccgctt ccatggcaaa
     9121 gccgctggta aacatctccg gtagaacgat cacatcgcgc ccggtaatac cttccagttg
     9181 acgatcaaaa tggcgcaggt tggcaggacc atccacccac accaacggtt gctgcaaaag
     9241 cgtaatcttc aaactaggca cggtgtacaa ctcctttatg cgaagggttt tataacttta
     9301 acaccttatc aggcagttgc cttagcgcag aataaattga taacaaatgc tgatattgga
     9361 aatatctgat ttgcaaatta tcgtgttatc gccaggcttt aggaggttaa taacatgggc
     9421 aggataagct cgggaggaat gatgtttaag gcaataacga cagtcgccgc actggtcatc
     9481 gccaccagtg caatggcgca ggatgattta accattagca gccttgcaaa gggcgaaacc
     9541 accaaagctg cgtttaatca gatggtgcaa gggcataagc tacctgcatg ggtgatgaaa
     9601 ggcggtactt atactcccgc acaaaccgta acgttgggag atgagacgta tcaggtgatg
     9661 agcgcgtgca aaccgcatga ctgtggctcg caacgtatcg ctgtgatgtg gtccgagaaa
     9721 tctaatcaga tgacggggct gttctcggct attgatgaga aaacgtcgca agagaaactc
     9781 acctggctga atgtgaacga tgcgctttcg attgatggta aaacggtgct gttcgcggcg
     9841 ttgaccggca gcctggaaaa ccatccggat ggctttaatt ttaaataatt agcggataaa
     9901 gaaacggagc ctttcggctc cgttattcaa ttacgcggct tcaactttcc gcactttctc
     9961 cggcaacttt accggcttcg tcgccagctc ttccggatca aagtcatcaa cgttaatact
    10021 gcgcagacgg ctttcttccg ctttcaccag aatagcggct tcatctttat caatcagccc
    10081 cttcgtcagc gcgttgtgcg ccagttcatc cagacgggta aatggcagat ttttacccag
    10141 ctctttacag atccgctgat gaatcgggtc ggcggcaatc acatccacca acgcctcttc
    10201 cagtaaacca accgggttat gctcgcttgg cgtcaggtac tgaccgcgac caatacgtga
    10261 acgagtggcg ttcggcactt gtaaaatctt cgccatttta tgatccagct tgtcagacgg
    10321 tgccagataa tgacgtccgg tcgggaagat caccacattc agcagcccgg caaccacgcg
    10381 gttcgggaag ttttgcagta aattgtccat tgcctgttct gcctgataca gcgcatcctg
    10441 tacgccccag tgcaccagcg gcagatcggc ttcattacgg ccttcgtcgt cataacgctt
    10501 cagaacggca gaggcgaggt aaagctggct taaaatatcc cccagacgtg ccgagatacg
    10561 ctcacgacgt ttcaggctgc cgcccagcag tgccatcgag acatcagaaa gcagggcgag
    10621 gttggcgctc aggcggttca ggtgctgata gtagcgttta gtggcatcgc cggttggcgt
    10681 gctgctggtt aaaccgcgcg tcaggcccag ccagaagctg cgaactttgt tgctaccgac
    10741 gtgaccgata tgtttgaaca acagtttatc gaacgcgttg acgtcattgt tcttcgccgc
    10801 ttccatctct tccagcacgt acggatggca acgaatcgct ccttgtccga agatcatcat
    10861 gctgcgggtc agaatgttag ccccttcaac ggtgatggca atcggtgcgc cctggtaagc
    10921 acgcgccagg aagttgcttt gcccgagcat aatgccttta ccgccggtaa tatccatcgc
    10981 atcaataatc gactgctgcc cgcggtgggt acagtgatac ttaacgatag ccgacagcac
    11041 ggcaggtttt tcgccgagca taatgccgta ggtaatcagc gatgccgcag catccattac
    11101 ataagcgttc ccggcaatac gcgccagcgg ctcttcaatc ccttccatct taccgataga
    11161 gattttgaac tgacggcgaa tgtgagaata cgcgcccgtt gccagcgcta ccgatttcac
    11221 gccgccggtt gagttggaag gcagggtgat gccgcggcct accgagaggc actccaccag
    11281 catccgccag ccttgcccgg ccattttcgg cccgccgatg atgtaatcga tcggcacgaa
    11341 gacatcttta ccgcgcgtcg gtccgttctg gaacggtacg ttcagcggga agtggcgacg
    11401 accaatttcc acgcccggcg tggtggttgg gatcagcgca caggtaatgc ctaaatcttc
    11461 tgcaccgccg agtaattttt ccgggtcgga gagtttaaac gccagcccaa gcacggtcgc
    11521 aatcggtgcc agcgtaatgt agcgtttgtt ccaggtcaga cgcatcccca gcacctgctg
    11581 gccctgccat tcgcccatgc agacaatccc ggtgtccgga atcgcgcccg catcggaacc
    11641 cgcttccggg ctggtcagtg caaagcaggg gatctcctga ccacgcgcca gacgcggcag
    11701 atagtgatct ttctgctcgt cagtgccgta atgttgcaac agttcgcccg ggcctaatga
    11761 gtttggcacg ccgacggtaa tcgccaggat cccgctcacg ccagagagtt tttgcagcac
    11821 acgagactgg gcataagctg agaactccag tccgccgtac tcttttttga taatcatcgc
    11881 gaagaagcga tgctctttaa ggtacgccca caactccggt ggcagatccg ccagctcatg
    11941 agtaatctgg aaatcattcg ccatccggca ggcttcttct accgggccgt cgagaaacgc
    12001 ttgctcttca gcggtcaggc gcggctgcgg atagttatgc agctttttcc agtccggctt
    12061 gccctggaac aagtcgccct cccaccaggt ggtgcccgca tcaatcgctt ctttctcagt
    12121 gcgcgacatc ggcggcatca ccttacggaa accgcgaaat accggcgcgg aaatcatcga
    12181 cttacgcata ggcgcaaagt taaatggcac gaggataatg gtcagaggca ccagtaccca
    12241 cgccgaccac agaccagcaa cgccgagggc ggctgtccag gcgagcaaaa tcagactgct
    12301 gataaataag ctcacgcggt gatagaacaa cgcgccgagc aggacaaccg tagcgagaat
    12361 actcaaaatc atcataacga aaagcccctt acttgtagga ggtctgacca cttgtgatga
    12421 tatggttgta gtggatgtaa aaacatttag caatgtgttt acaatataat tacaacaaag
    12481 ctcacattgt tgctgttttt atccgcactt caggtcaaaa agtcctggtc atagcacctg
    12541 cccgtacttc tcgcttttgg cggtatccgg tacactgcat tttgtctatt acatttatgc
    12601 tgaaggatat cctcatgtac caggatctta ttcgtaacga actgaacgaa gcggcggaaa
    12661 cgctggctaa ctttttaaaa gatgacgcca atattcacgc cattcagcgc gcggcggtcc
    12721 tgttagcaga cagctttaaa gccggtggca aagtgctttc ctgcggcaac ggcggttccc
    12781 attgcgacgc tatgcacttt gccgaagagt tgaccggtcg ctaccgtgaa aaccgtccgg
    12841 gctacccggc gattgctatt tctgacgtta gtcatatttc ctgcgtcggt aatgatttcg
    12901 gtttcaatga tattttctcc cgctacgttg aagcggtagg tcgcgaaggc gatgtactgc
    12961 tggggatctc cacctccggt aactctgcaa acgtgatcaa agcgatcgca gcggcgcgtg
    13021 agaaggggat gaaagtgatc accctgaccg gtaaagacgg cggtaaaatg gctggcacgg
    13081 cggatatcga aattcgcgtt ccgcactttg gttatgccga ccgcattcag gagattcaca
    13141 ttaaagtgat ccatatcctg atccagttaa ttgaaaaaga gatggttaag taagtctggc
    13201 gtaggccgga taaggcgttt acgccgcatc cggcacttgt gcgctgatgc ctgatgcgat
    13261 gctgacgcgt cttatcatgc ctacaaatct gtacccgaac cgtaggccga ataattcgtt
    13321 tacgccgcat ccgacctgaa aattcttaaa tcaatcttcg ccggggtcca tgcgctcccg
    13381 ctgttgtgga ggttacccat gtgcgaattg ctcgggatga gcgccaacgt ccctaccgat
    13441 atctgcttta gtttcaccgg acttgtacag cgtggtggtg gaaccgggcc acataaagat
    13501 ggctggggca ttacctttta cgaaggtaaa ggctgtcgca catttaaaga tccacaaccc
    13561 agctttaatt cccccatcgc caaacttgtc caggactacc cgataaaatc ctgttcggtg
    13621 gtggctcata ttcgccaggc taatcggggc gaggtggcgc tggaaaatac tcacccattt
    13681 acccgcgagt tatgggggcg taactggact tatgcccata acggacaact gacgggctac
    13741 aaatcactgg aaaccggcaa cttccgcccg gtcggtgaaa ccgacagcga aaaagccttt
    13801 tgctggctgc tgcataaatt aacgcagcgt tacccgcgca cgccgggcaa catggcggca
    13861 gtgtttaaat atatcgcctc actggcggat gaactgcggg cgaagggcgt tttcaacatg
    13921 ctgctttcgg acgggcgcta tgtaatggcg tattgttcga ctaatttaca ctggatcacc
    13981 cgccgcgcgc cgtttggcgt ggcaacgttg ctggatcagg atgtggaaat cgacttcagc
    14041 tcgcagacca caccgaatga tgtggtcacg gtgattgcga cacagccgct gacgggcaat
    14101 gaaacctggc aaaaaattat gccaggcgaa tggcgcttat tttgcctcgg ggagcgtgta
    14161 gtttgatgcc agttgtggct gcaccacttc gtggcttaac ggcttgctga ccacgtaacg
    14221 gccattggcc acagaaacgg tcggtggctt acgggtttgc tcaaagtagt cgtagcccgg
    14281 cttcagctgc tcccagaagt ccttaaagtt ggaatattta tggcgcttca tattggcgtc
    14341 ggtcatgcgg aatgggtaaa tactcacctg cacgctcggc tgaccaaaca ccagcgcacc
    14401 agtaacgaac tggaagatct catcaatacc ctgattggtc attgcgtagc agccgatgga
    14461 tacacaatcg ccgtggatca tcaggtattt cccttcataa ccatgcgcac ggtcataagc
    14521 attggggaat ccaatattaa tcgctttgta gtaacggctg tctggtttta actgattacg
    14581 ctggacgcta taaaaccctt ccgggctttt gaaatcgccc tgacgctgtt ttggccctaa
    14641 gccgccggaa tatttacaga ttttataact gtcgagcagt tgatattgct cgcccatttt
    14701 gacgtaaaga tcgagcgtac gttcttcctt gaagatctgg atgtagacag gggatcccat
    14761 caactgctgc ttatactctt tgctcactgg cgtggtcgag ctactgctgc ccagcaaacc
    14821 ggcaaacgaa acgcacggga tcaacaacat cgcaagaatt aatgcgattt tacgcatact
    14881 gcttattcct tgataaaacg gttacacacg ccaggacggc aaaatggatc ccaaatcgga
    14941 atagtctgga tttggaaggc tcacattatc accaaaagag ttttacgcaa gcctgtcggc
    15001 gctgggttta caaatttagc acaggggcag ttttcaatag tccgaaaagc gcatgtatca
    15061 tttatttcct aatattatga aatctcaata gtaatatgca aataagcaat taataatccc
    15121 gtttactcct ccggtctttt aaaattctca ccttcattca gccggtttat gtctttcatg
    15181 tcttcgttct tgctcggcag atttttgcat ccaggcgtgt tttcgttatg cgttttgctc
    15241 cctttactcg ccagcgccac cacaaccggg agcagataaa acagcatcca gaatggtttc
    15301 ctgcgccgct caaggcgagt gacagacgct ggcaggcgct ggcggaaaac aaccactttt
    15361 taagcagcga tcacctgcat aacatcaccg aagtggcgat tcaccgcctg gagcagcagc
    15421 ttggcaagcc ttacgtctgg ggcggcacgc ggcccgatca aggcttcgat tgtagtgggt
    15481 tagtttttta tgcctacaac aagatccttg aggcgaaact cccgcgcacg gccaatgaga
    15541 tgtaccacta ccgtcgggca acgattgtgg cgaataacga cctgcgccgg ggagatttgc
    15601 tatttttcca tatccacagc cgcgagattg ccgaccatat gggggtgtat ctgggcgacg
    15661 gtcagtttat tgagtcactg cgcaccgggg aaaccattcg ggtaagccgg ttagccgaac
    15721 ctttctggca ggaccatttt ttaggcgcgc gcaggatttt gacggaagag atgattttgt
    15781 aggccggata aggcgtttac gccgcatccg gcggttgtgc acggatgcct gatgcgacgc
    15841 tgacgcgtct tatcatgcct acaaatctgt gcgtttttgc gagccgcttt cccgatataa
    15901 cacagcgtca ttgtcggcct ttccttttcc tcttaccgtt gagaaaaagg agtcgctatg
    15961 tcggaatatc gtcgttatta catcaagggg ggaacatggt ttttcacggt aaatttacgc
    16021 aatcgtcgaa gccatcttct gaccacccag ttccagacgc tccgtaacgc cattattaac
    16081 gttaagcgag acagaccttt tgaaattaac gcctgggtcg ttttgccgga gcatatgcat
    16141 tgcatatgga cattacccga aagcgatgat gatttttcct ctcgctggcg ggagattaaa
    16201 aagcagttca cccatgcttg cggattgaaa aatatctggc aaccgcgttt ttgggagcat
    16261 gccattcgca atacaaaaga ttatcgccat catgttgatt atatttatat aaatccagag
    16321 aaacatggct gggtaaagca ggtgagtgat tggccattct caacgttcca tcgcgatgtc
    16381 gcgcgagggt tatatcccat cgattgggcg ggagacataa cggatttaag tgccggggag
    16441 cgtatcattt tataactgtg cgctggtgcc ggatgcgctt acgcttatcc ggcctacaaa
    16501 tcgtgcttta acggtaggcc ggataaggcg ttcacgccgc atccggcagt tatgcgcaga
    16561 tgcctgatgc gacgctggcg cgtcttatca tgcctaaaaa cttgtgcctg aacggtaggc
    16621 cggataaggc gtttacgccg catccggcaa ttgtgcaccg atgccggatt cttacatcaa
    16681 cgcccccata atcttcaact ccagctcatc cggcacttcg ttatacgaca gcacatgcag
    16741 ccccggcgca aacaaccttg cataacgcgg cagcaaaggg cgtagctgcg gcggcaccag
    16801 cagcaccgga tctttccccg ccgctttcat ctgctctttc acctgtggca tcgtgctctg
    16861 gaactggttg agcatattcg ggtcaaccgg cacgctgtcg agcatcactt tgccggcctg
    16921 ttgcgcctga ttcactacgt tggtcagcag attttccagc tcattattca gcgtatacac
    16981 cgtcagctcc tgcttgcgaa cgaacggatg agtaatgctg cgccgcagcg ccaggcgcac
    17041 atcggccgcc agcagaatat gatctttagt caccgtgcta ctggcgacca gcacggtggc
    17101 gatggtgacg atatcgcgca gggaaacgcc ttcggtcagc agcgcccggt acactttcag
    17161 caattggctg taattgagcg ccgcgcttaa atcctccgcc aggcgcggtg ccatcgacga
    17221 caaacggtta tgcaactgcg tgatgtcatc atagttaaac aaatcaggaa tatagctgcg
    17281 cacaatcttg ttcacatggg tggcaatcac actggcgctg tcgatcacct gataccccat
    17341 attcagagcc ttcgctttct gcgcaggctg gatccaggtg accggtatcc cgtacgccgg
    17401 gtcgttcccc agcacgccgt caatctcgcc gtaggtttcg ctggagggca gcgccatcag
    17461 tttatccgcc ggaatatccg cttcatcagc ctcaatgccg ttgatgaaaa tagcgtactg
    17521 gctgggcttg aggcggaagt tttcccgaat gcggatctcc ggcagcagca cgccgttacc
    17581 gtcggaaatc acctgccgaa cgccgcgaat ccgctgggtg agcgggttgc cctgcgcttt
    17641 gtccaccagc gccaccagct tgtagccgag gcttaagctg atgggctcga tcagcggaat
    17701 agtttcccaa ctgacctgtt gttcgctggt ttcagtgatg gtgcgggtca gcgtttcgag
    17761 gcttttctct tccgcctccg ccgcctgcgg gcgtttgctc atccgccagc cggtaaagcc
    17821 aagcagggcg ctgaacagca aaaacggcag atgcggcatc cccggcacca ccgccagcac
    17881 gaacatgatc ccggtggcgg tgtagagcac cgacgggctt gccagcaact ggtggcgcac
    17941 gtcatgggcg atatcgccgt tgtcgctgac gcgggtgacg ataatcgccg ccgcggtgga
    18001 gagcagcagg gaagggatct gcgccaccag gccgtcgccg atggtcatca gcacgtattg
    18061 ctggaaggcg gcgtcggcgc tcaggttgta tttgaagatg ccgatacaga cgccgccgct
    18121 caggttgatc gccagaatca tcatcccggc gatggcgtct ccgcgtacaa acttcgatgc
    18181 cccgtccatc gcgccgtaga aatcggcctc gctggcaaca tctttacgcc gggtttgcgc
    18241 ctgcgcctgg ttgatcaatc cggcgttaag atcggcgtca atcgccatct gtttgccggg
    18301 catcgcgtcc agggtaaagc gggcagaaac ctcggaaata cgctcggcct ctttggtgac
    18361 gacgataaag ttgattgtca acagcgtcag taaatccgag cgtgaaagca ttatcgccgc
    18421 gctgcacggg cagtcgattt ttaacggtgg tgggctgtcg ccgttaaata aaatcagccc
    18481 gtctcatccg ccaaaacctg ccaccgtggc ggtgccggaa gagacggaga aaaaagcgcg
    18541 tgatgtgaat gagaaaacgg cgctgctgaa gaagaaatcc gccaccgagc ttggcgagtt
    18601 ggcaaccagt atcaacacca ttgcccgtga tgcgcatatg gaagcgaatc tggagatgga
    18661 gattgttccc cagggattac gcgtgctgat taaagacgac cagaatcgca atatgttcga
    18721 acgcggcagc gcgcagatta tgccgttctt taaaactttg ctggtggagc tcgcgccagt
    18781 gttcgactcg ctctataaca aaattattat taccgggcat accgatgcga tggcctacaa
    18841 aaacaatatc tacaacaact ggaacctttc gggcgatcgc gcgctttcag ctcgtcgggt
    18901 gctggaagag gccggaatgc cggaagataa agtgatgcag gtaagcgcaa tggcggacca
    18961 gatgctgctg gacgcgaaga atccgcaaag cgcgggcaat cggcgtattg agattatggt
    19021 gctgaccaaa agtgcgtccg atacgctgta tcaatacttt ggtcagcatg gggataaagt
    19081 ggtgcagccg ctggtgcaaa agctggataa gcagcaggtg ctttcgcagc gaatgcgcta
    19141 aatgctgaat ctttacgcat ttctcaaaac ctgaaatcac tgtatacttt accagtgttg
    19201 agaggtgagc aatgcgtaaa atcattcatg tggatatgga ctgctttttc gcagcggtgg
    19261 agatgcgcga caatcccgct ctgcgcgata tccctattgc tattggcggc agccgcgaac
    19321 gtcggggggt gatcagcacc gccaattatc ccgcgcgtaa atttggcgta cgtagcgcta
    19381 tgcctactgg gatggcgctc aaattatgcc cacatctcac cttgcttccg gggcgctttg
    19441 acgcctacaa agaagcctca aatcatatcc gtgaaatctt ctcgcgctac acctcgcgca
    19501 ttgaaccgtt gtcactggat gaggcttatc tcgatgtcac cgatagcgtc cattgccacg
    19561 gttctgcgac cctcatcgcc caggaaatcc gccagacgat cttcaacgag ctgcaactga
    19621 cggcatctgc gggcgtggca ccagtaaagt ttctcgccaa aatcgcctcc gacatgaata
    19681 aacccaacgg tcagtttgtg attacgccgg cagaagttcc ggcattttta caaaccttac
    19741 cactggaaaa aatccccggc gtcggcaaag tctcggcggc aaaactggaa gcgatggggc
    19801 tgcggacctg cggtgatgta caaaagtgtg atctggtgat tctgcttaaa cgctttggca
    19861 aatttggccg cattttgtgg gagcgtagtc aggggattga cgagcgcgac gttaacagcg
    19921 aacggttacg aaaatccgtc ggcgtggaac gcacgatggc ggaagatatt catcactggt
    19981 ctgaatgtga agcgattatc gagcggctgt atccggaact tgaacgccgt ctggcaaagg
    20041 taaaacctga tttactgatt gctcgccagg gggtgaaatt aaagttcgac gattttcagc
    20101 agaccaccca ggagcacgtc tggccgcggc tgaataaagc tgacttaatc gccaccgcgc
    20161 gtaaaacctg ggatgaacgc cgcggcgggc gcggtgtgcg tctggtgggg ctgcatgtga
    20221 cgttgcttga cccgcaaatg gaaagacaac tggtgctggg attatgatgt atactattat
    20281 gtatattctg gtgtgcatta ttatgagggt atcactgtat gcatcgaatt ctcgctgaaa
    20341 aatcggtcaa tatcactgag ttacgtaaaa acccagctaa atactttatt gatcaaccag
    20401 ttgcggttct ttctaataat cgccccgcag gatatctctt aagtgccagc gcattcgaag
    20461 cgttaatgga catgcttgct gaacaagagg agaaaaagcc cataaaggcg cgctttcgtc
    20521 caagtgctgc aagattagag gaaattacac gccgcgctaa acaatatctt aatgatatga
    20581 cggatgatga tttcaatgac tttaaggaat aaggatgcgg gtattcaaaa caaaacttat
    20641 tcgcctgcaa cttacagcag aggaacttga tgcgttaacg gcggatttta tttcctataa
    20701 gcgtgacggt gttttgccag atatatttgg tcgcgatgca ctctacgacg actcgtttac
    20761 ctggccatta atcaaatttg agcgagttgc tcatattcat ctggcaaatg tgaataatcc
    20821 atttccgcca cagttgcgcc aattcagcag aacgaatgac gaagcgcatt tggtatattg
    20881 tcagggtgcg tttgatgagc aagcatggtt gctcattgcc attctgaagc ctgaacctca
    20941 taaactggct cgagataaca accaaatgca taaaattgga aaaatggcag aagcgtttcg
    21001 catgcgtttt tgaatttata ttatgaataa catacaaata agagattatc agcctggcga
    21061 ttttcagcaa ctatgcgcta ttttcattag agcggttacg atgaccgtca gtcagcatta
    21121 ttcactacaa caaattgccg cctgggcgca gattgacgaa tctcgctgga aggagaaact
    21181 cgcgaaatca caagtgcggg ttgcagttat taatgcaaaa ctggtcggct ttattacctg
    21241 cgttgaacac tatatcgata tgttatttgt tgaccctgaa tacacccgcc gtggggttgc
    21301 cagcgctttg ttaaaacctt ttattaagtc tgaatccgaa cttacggtgg acgcaagcat
    21361 aaccgcaaaa cccttttttg aacgttatgg ttttcagaca gttaagcagc agcgcgttga
    21421 atgccgggga gcgtggttta ttaattttta tatgcgttat aaaccgcaac attaaatcca
    21481 gcttgcaatg aaaataacgc ccgcctagta tgtgcgcgtt ttcttattcc acaaactgca
    21541 aggaggtaaa tcatgacaaa tcctttatca atgactcttt gcagaccttt ccaggattaa
    21601 ttcttttttt cttgccctgg attcgtctgc catttcctga ttttttaatt gttactctca
    21661 ataattcaag tcgcagcaca tgcaacttga agtatgacga gtctatttac ggaatggtat
    21721 atgggcaatt atattcgtcc cttatccgat gcggtattta ccatcgcatc tgatgacctg
    21781 tggatcgaga gtttagcgat ccaacaatta cacaccacgg caaatttacc caacatgcag
    21841 cgcgtagtgg ggatgccaga tttacacccc ggacgcggct acccgattgg cgcagcgttc
    21901 ttctccgcag gccgttttta cccagcactg gtcggcaatg atatcggctg tggcatggcg
    21961 ctatggcaaa cagatattct cgctcgcaaa tacaacgccg ataagtttga aaagcgatta
    22021 tcagctttgg atgacgttgc tgaagaaagc tggctggagg aaaacctgcc gtcagcattt
    22081 gcacagcatc cgtggcgcag ctcgcttggc tctatcggtg gtggcaatca tttcgctgaa
    22141 ctgcaacaag ttgatcaaat catcaacgct gaactgtttg cactggcagg tctggatgcg
    22201 cagcatctgc aactgctggt tcatagcggc tcgagggggc tagggcagtc tattttacag
    22261 cggcatattg cctcgttttc gcatcatggt ttgcctgaag gcagtgacga cgcgctggcg
    22321 tttgcgcgtt ttaatcgcca cctgatcgct ttgcgcataa tgcaacaggt taaggccacc
    22381 ggaagtccgg ttctggatgt ggcgcataac tttgttagcg cgtgtcgaat cggtgatcaa
    22441 caaggggtct tacatcgtaa aggtgccacg cctgatgact gtggtctggt ggttattccc
    22501 ggttcacgcg gtgattactc caggctggtt cagcccgtca ggagtgagga aacattgcat
    22561 tcgctggcgc atggggctgg gcgtaaatgg gggcgcaccg agtgtaaagg gcgtctggca
    22621 gcgaaataca cagcgacgca actctcgcgt actgaacttg gcagtcgggt aatttgtcgc
    22681 gataaacaac tcatcttcga agaagcgcca caagcctata aatcggctga aagtgtggtg
    22741 caatgtcagg gcaggccgga ttaattattc ctgtcgcgcg gctgcgtccg gtgctaacgc
    22801 tcaaaaacag tggagggaaa aaaggatgat cttgctataa ctctcctcag cgcaggggcc
    22861 ggaagaatgt tgtctcgcag tgaaaaaggc gctggacagg ctaattaaag aagctgcccg
    22921 acaagacgtc gcggtaacgg tgctggaaac agaaacaggc cgctactctg acacgctgcg
    22981 ttcggcgctg atttctctgg atggcgacaa cgcatgggcg ttaagcgaaa gctggtgtgg
    23041 cactattcag tggatttgcc cgagcccgta tcgcccacat cacgggcgta aaaactggtt
    23101 tctgggcatt gggcgtttta ccgctgatga gcaggagcaa tccgatgcga tccggtatga
    23161 gacgctgcgt tcgtcggggc cgggcggtca acatgtcaat aaaaccgact cggcggtacg
    23221 cgccacgcat ttggcatccg gtattagcgt gaaggttcag tcagagcgca gccagcatgc
    23281 taacaaacgg ctggcgcgat tgctgattgc ctggaagctg gatcagcagc aataggaaaa
    23341 tagcgcggtg ctgaaatcac agcggcgaat gttccatcac cagattgaac gtggcaaccc
    23401 gcgacgaacg tttacaggga tggcttttat cgaaggataa tgaaggatga aactgccgga
    23461 aggcgattaa acgccatccg gcagcaaatc aaataattac ttcgccggaa tttctttcag
    23521 cagttcagtc agcagtgtcc agtaatgacc tacgctttcg atgtgaactt gctcatccgg
    23581 agagtgtgga ccggtgatag ttggcccgat agaaaccatg tccatttccg gatacggttt
    23641 tttgaacaga ccacattcca ggcccgcgtg gataatctgg atgttcggcg tcttgttgaa
    23701 caggcgctga taggtttcac gtaccagatg catcaccgga gaattagcgt ccggctgcca
    23761 gccaggatat gcgcctttcg cttcggtttt cgcgccagcc agtttaccca gcgaatccag
    23821 catgctcacc acgtagtctt taccgctgtc gattagtgaa cggatcaggc agtgaatttc
    23881 tacgttattg tcagtcatgg tcaccacacc gacgttcagg gaggtttcga ccacaccttt
    23941 tgccacgtcg gaattgcgga tcacaccgtt cggggtggcg ttcagcagac gaataaaggt
    24001 atcgcgagat ttcgcaatca gggcagcttt atcgttcgct acagagtcca gcaacaaggc
    24061 cagatttttc tctttctctg ccagctcgtt tttcaggatc tcctgatagg tattcaccag
    24121 agatttcagt gcgtcgactt tatcagctgc gacagcaatg gtcgcaaagg cttcacgcgg
    24181 gatggcgtta cgcagtgtgc cgccgttgaa atcgataagg cgcaggtcca gttcttctgc
    24241 atgacccgcc aggaagcgca ccagcagttt gttggcatta cccaggccaa cgtggatttc
    24301 accgccggag tgaccgcctt tcagaccttt taaggttaac ttgaaggttt caaaaccagc
    24361 tggaaccgct tcacgatcta aatgcaggtt ggaggtgaag tcgatacccc ccgcacaacc
    24421 catgtagatt tcaccttctt cttcggagtc ggtgttaatc agaatatcag cctgcaacca
    24481 gttgctctgt aagccgaacg caccgtccat accggcttct tcggtcatgg tcagcagcac
    24541 ttccagcggg ccgtgaacca cgttttcgtc agccagaacc gccagtgcag aggccatacc
    24601 aatgccgtta tccgcaccca gcgtggtgcc gcgcgcttta acccattcgc catcaatata
    24661 aggctggata ggatctttcg tgaagtcatg cacggtgtcg ttatttttct gcggcaccat
    24721 atcgaggtgg gcctgtaaga cgaccggttt acgattttcc atacctgcgg tagcaggttg
    24781 acgaatcagg atattaccta cctgatcgcg ttcgacatgg aaacctttct cttttgccca
    24841 accaacaatg tattcagcga gttgctcttc atgataggac gggtgaggaa tagaacagat
    24901 tttggcaaaa atatcccaca gcggctgtgg agataattga gacagttcag acacgttaag
    24961 tctccttgtc gatcacccgc aaaacagtat tgcaggtcac agggttagca gaaaatgttg
    25021 tcaacacaag acaggcttgc gagatatgta ttgagaatac cactttatcc cgcgtcaggg
    25081 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
    25141 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
    25201 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
    25261 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
    25321 agccgtggcg gtctggtacc gggggcgtta ctggcgcgtg aactgggtat tcgtcatgtc
    25381 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa
    25441 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
    25501 actgctgttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa
    25561 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatc
    25621 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
    25681 caacgcctgg cattgccggg cgttgttctt tttaacttca ggcaggttac aatagtttcc
    25741 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
    25801 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca
    25861 accgcctgtg cagtcggctc ttgatggtaa aaccatccct cactggtatc gcatgattaa
    25921 ccgtctgatg tggatctggc gcggtattga cccacgtgaa atcctcgacg tccaggcacg
    25981 tattgtgatg agcgatgctg aacgtaccga cgatgattta tacgataccg tgattggcta
    26041 ccgtggtggc aactggattt atgagtgggc cacccaggcg atggtgtggc aacaaaaagc
    26101 ctgtacggaa gaagatccgc aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta
    26161 caacattgcc gcctatcctc atctgaaagg agatgacctg gccgagcaag cgcaggcttt
    26221 atcaaaccgc gcctatgaag aggccgccca gcgtctgccg ggcacgatgc ggcagatgga
    26281 gtttaccgta cccggcggtg cgcccatcac cggctttttg catatgccga aaggcgatgg
    26341 cccgttcccg acagtattaa tgtgtggtgg tctggatgcg atgcagacgg actattacag
    26401 cctgtatgaa cgttattttg cgccgcgcgg cattgcgatg ctgactattg atatgccgtc
    26461 ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc agcctgttgc atcagcacgt
    26521 cttaaaggcg ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg cctttggttt
    26581 ccgtttcggc gctaacgttg ccgtgcgtct ggcatacctt gaatcgccgc gtctgaaagc
    26641 ggttgcctgt cttggtccgg tagttcatac cctgttgagt gattttaagt gccagcaaca
    26701 ggtgccggaa atgtatcttg acgttctggc gagtcgtttg gggatgcatg atgcttccga
    26761 cgaagcgtta cgcgtggagc tgaatcgcta ttcattaaaa gtgcaaggat tgctggggcg
    26821 tcgctgccca acgccaatgt tatcaggcta ctggaagaac gatccgttca gcccggaaga
    26881 ggactcacgc ttaatcacct catcatctgc tgacggtaaa ttattagaga tcccatttaa
    26941 cccggtgtat cggaattttg acaaaggtct tcaggaaatc accggctgga tcgaaaaacg
    27001 cttgtgttaa aaatttgcta aattttgcca atttggtaaa acagttgcat cacaacagga
    27061 gatagcaatg acgttaccga gtggacaccc gaagagcaga ttgatcaaaa aatttaccgc
    27121 actaggcccg tatattcgtg aaggtaagtg cgaagataat cgattctttt tcgattgtct
    27181 ggctgtatgc gtcaacgtga aaccggcacc ggaagtgcgt gaattctggg gctggtggat
    27241 ggagctggaa gcgcaggaat cccgttttac ctacagctac cagtttggtc tgttcgataa
    27301 agcaggcgac tggaagagtg ttccggtaaa agatactgaa gtggttgaac gactggagca
    27361 caccctgcgt gagtttcacg agaagctgcg tgaactgctg acgacgctga atctgaagct
    27421 ggaaccggcg aatgattttc gtgatgaacc ggtgaagtta acggcgtgag tgaaatgtgc
    27481 cggatgcatc acatccggca atattcatta aaactgatac gtcatcccaa ccgcgacagt
    27541 atcatcatta ttaatattca gcttattatc gctatccagt tggttgattt tataatcaac
    27601 aaacgctgac atatttttgt tgaaataata cgtagcaccg acgtcgatat aattgaccag
    27661 atcttcatca ccgatgccct caatatcttt ccctttcgat aagacataac ccagcgatgg
    27721 acgcagacca aagtcaaact ggtattgagc gaccgcttca aagttctgtg tcttattggc
    27781 aaagccgcca gttattggcg tcattttgcg tgtttcagaa tagaaagttg ccagataaat
    27841 attattggca tcgtatttca gacctgttgc ccatgcttct gcacgcttgc ctgtgccacg
    27901 gctttgcagg ttctgctcgt tggtgcgatc tgagttggta taggccccac taatggcgaa
    27961 atcgctgccg ccaaagtcat atgtcaatga cgtgccgaag ccatcgccgt tttgcttttt
    28021 aacgtcgcgg ttttcgtttt tcccttgata ttgcagggtt aagttcaggc catcgataac
    28081 gccgaagaag tcggtgttcc gatacgtcgc cagaccgctg gcgcgtttgg tcataaagtt
    28141 gtcggtctgc gccgaggaat cgccaccaaa ttccgggaac atatcggtcc aggcttccac
    28201 gtcatacaac gcgcccaggt tacgaccata atcgaaagaa cccaaatctt tatatttcaa
    28261 cccggcaaaa gcgagacgcg ttttttgctg tgcagtatca ctctccgctt tatttccggc
    28321 aaactccgct tcccaccgac cataaccagt cagttgatcg ttaatttgag tttcaccttt
    28381 aaaaccaaaa cggatataac tctggtcgcc atctttactg gcgttatcac tcatataatg
    28441 catggcttta actttgccat agacatccag tttattaccg tctttattat atatttctgc
    28501 agcctgtaca gatgcagatg ccacaatgcc catcaccact aatgccagag tgctcttttt
    28561 cattttcatt cctgatttta attaacgcgc gaatattcag cgggagagcc ccgttgaaaa
    28621 caggaaagtt tttaacctga gattgttaaa gatatattac agattaatga tatt
//
LOCUS       DAJTRD010000048        28425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTRD010000048 DAJTRD010000000
VERSION     DAJTRD010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.048"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            564..1334
                     /locus_tag="OO315_002277"
     CDS             564..1334
                     /locus_tag="OO315_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5891169.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO315_002278"
     CDS             1325..1798
                     /locus_tag="OO315_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5891170.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO315_002279"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO315_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5891171.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO315_002280"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO315_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5891172.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO315_002281"
     CDS             3233..3727
                     /locus_tag="OO315_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5891173.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3967..6258
                     /gene="opgB"
                     /locus_tag="OO315_002282"
     CDS             3967..6258
                     /gene="opgB"
                     /locus_tag="OO315_002282"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5891174.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6396..7418)
                     /gene="lgoD"
                     /locus_tag="OO315_002283"
                     /pseudo
     CDS             complement(6396..7418)
                     /gene="lgoD"
                     /locus_tag="OO315_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7557..8471
                     /locus_tag="OO315_002284"
     CDS             7557..8471
                     /locus_tag="OO315_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5891175.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8686..10047
                     /locus_tag="OO315_002285"
     CDS             8686..10047
                     /locus_tag="OO315_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5891176.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10096..11760)
                     /gene="tsr"
                     /locus_tag="OO315_002286"
     CDS             complement(10096..11760)
                     /gene="tsr"
                     /locus_tag="OO315_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5891177.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11879..12325)
                     /gene="hpaR"
                     /locus_tag="OO315_002287"
     CDS             complement(11879..12325)
                     /gene="hpaR"
                     /locus_tag="OO315_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5891178.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12597..13886
                     /locus_tag="OO315_002288"
     CDS             12597..13886
                     /locus_tag="OO315_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5891179.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPSDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13883..15349
                     /gene="hpaE"
                     /locus_tag="OO315_002289"
     CDS             13883..15349
                     /gene="hpaE"
                     /locus_tag="OO315_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5891180.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15351..16202
                     /gene="hpaD"
                     /locus_tag="OO315_002290"
     CDS             15351..16202
                     /gene="hpaD"
                     /locus_tag="OO315_002290"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5891181.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16212..16592
                     /locus_tag="OO315_002291"
     CDS             16212..16592
                     /locus_tag="OO315_002291"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5891182.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16660..17463
                     /gene="hpaH"
                     /locus_tag="OO315_002292"
     CDS             16660..17463
                     /gene="hpaH"
                     /locus_tag="OO315_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5891183.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17474..18277
                     /gene="hpaI"
                     /locus_tag="OO315_002293"
     CDS             17474..18277
                     /gene="hpaI"
                     /locus_tag="OO315_002293"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5891184.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18299..19654
                     /gene="hpaX"
                     /locus_tag="OO315_002294"
     CDS             18299..19654
                     /gene="hpaX"
                     /locus_tag="OO315_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5891185.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCLTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19664..20554
                     /gene="hpaA"
                     /locus_tag="OO315_002295"
     CDS             19664..20554
                     /gene="hpaA"
                     /locus_tag="OO315_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5891186.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20581..21518
                     /locus_tag="OO315_002296"
                     /pseudo
     CDS             20581..21518
                     /locus_tag="OO315_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21745..23303
                     /gene="hpaB"
                     /locus_tag="OO315_002297"
                     /pseudo
     CDS             21745..23303
                     /gene="hpaB"
                     /locus_tag="OO315_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23321..23833
                     /locus_tag="OO315_002298"
     CDS             23321..23833
                     /locus_tag="OO315_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5891187.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24226..26376
                     /gene="btsT"
                     /locus_tag="OO315_002299"
     CDS             24226..26376
                     /gene="btsT"
                     /locus_tag="OO315_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5891188.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26426..26629
                     /locus_tag="OO315_002300"
     CDS             26426..26629
                     /locus_tag="OO315_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5891189.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26640..27596
                     /gene="yjiA"
                     /locus_tag="OO315_002301"
     CDS             26640..27596
                     /gene="yjiA"
                     /locus_tag="OO315_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5891190.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27665..>28425)
                     /locus_tag="OO315_002302"
     CDS             complement(27665..>28425)
                     /locus_tag="OO315_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR5891191.1"
                     /translation="NYTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMART
                     EDFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQ
                     HADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQ
                     ALVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSF
                     YLTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6955 a         7427 c         7127 g         6916 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaatgtt acccggctga cgcggctctc tcctgccctt ttccctgctc
     3781 ctggacggtt ttccccctgt tacacgggaa gcgaccgcga tcaaaaaagt ctgtttataa
     3841 actaattcct aacaacatcg catttttttg acactagaat tgcagcaata tggcgacgcc
     3901 attacattgt gcttctcaaa aagcttaaac gttggctaaa cggcccggtt tagtgaggat
     3961 cgcccattgt cagaactact ctctttcgcc ctttttctcg cctctgtgct gatttacgca
     4021 tggaaagcgg gacgtaacac ctggtggttt gcagccacgt taacggtgct ggggctattt
     4081 gtcgttttaa atatcaccct gtttgccagc gactatttta ctggcgatgg tattaacgac
     4141 gcggttctct ataccttaac caacagcctg accggtgctg gcgtcagcaa atacatcctg
     4201 ccgggtatcg gcattgtgct ggggctgacg gcggtgttcg gtgcgctggg ctggatcctg
     4261 cgccgtcgtc gtcatcatcc gcaccatttt ggttacagcc tgctggcgct cttactggcg
     4321 ctgggttcgg tggacgccag cccggcattt cgtcagataa cggaactggt gaaatcccag
     4381 tcacgcgatg gcgacccgga ctttgcggct tattataaag agccgtcgaa aactatccct
     4441 gacccgaaac tcaacctggt ttatatctac ggcgaaagtc tcgagcggac ctattttgat
     4501 aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt tgaaaaatga aggcctggat
     4561 ttcagccaca cgcagcagct gccaggaacg gattacacga ttgcgggcat ggtggcttct
     4621 cagtgcggca taccgctgtt cgcccccttt gaaggcaacg cctccgcctc tgtctccagc
     4681 ttcttcccgc agaacatctg tctgggcgat atcctgaaaa actcgggtta tcagaactat
     4741 ttcgtgcagg gcgcaaatct gcgttttgcc ggtaaagatg tgttcctgaa atcgcacggc
     4801 ttcgaccact tatacggctc agaagagctg aaaagcgtgg tagccgaccc gcactatcgc
     4861 aacgactggg gattctacga cgataccgtt ctcgatgaag cgtggaaaaa gtttgaagag
     4921 ctttcccgct caggtcagcg attctcactg tttaccctga cagtcgatac ccatcacccg
     4981 gatggtttta tctcgcgcac ctgtaaccgc aaaaaatatg attttgacgg taagccgaat
     5041 cagtcattca gcgcggtaag ttgcagccag gagaatatcg cgacgtttat caacaaaatc
     5101 aaagcgtcac cgtggtttaa agatactgtc atcgtcgtct cttctgacca tttagcgatg
     5161 aacaacacgg cgtggaaata cctcaataag caggatcgca ataacctgtt ttttgtcatt
     5221 cgtggcgaca agccgcagca aaagacgctg gcagtgaagc gtaacacgat ggataacggc
     5281 gcgacggtgc tggacattct cggtggcgat aactatctcg gacttggtcg tagcagttta
     5341 tccgggcagt cgatgtcgga aatcttcctc aatatcaaag agaaaacatt ggcgtggaag
     5401 ccagatatca tccgcctgtg gaaattccct aaagagatga aagagttcac catcgaccag
     5461 cagaaaaaca tgattgcctt ctcgggtagc catttccgtt tgccgctgct gttgcgggtt
     5521 tcagacaaac gcgtggaacc gctgccggaa agcgaatact cagcaccgct gcgtttccag
     5581 ctggccgatt tcgctccacg cgacaatttc gtctgggttg acagttgcta caagatggca
     5641 caactctggg ctccggaact ggcactttcc accgactggt gtgtctcgca ggggcagctt
     5701 ggcggtcagc aaattgttca gcatgttgac aaaacaacat ggaagagcaa gacggcattt
     5761 aaagatacgg tcatcgacat ggcgcgttac aaaggcaatg tcgatacgct gaagattgtt
     5821 gataacgata ttcgctacaa agccgacagt ttcatcttta acgtcgccgg tgcgccagaa
     5881 gaggtgaaac agtttagcgg gatttcccgt ccggagtcgt ggggccgctg gtccaacgcg
     5941 cagctgggcg atgaagtaaa aatcgagtac aagcatccgc tgccgaagaa atttgacctg
     6001 gtgattaccg ccaaagcata cggcaataac gccagccgtc ctattccggt acgcgtaggc
     6061 aatgaagaac aaactcttgt gctgggcaat gaagtgacca ccaccacact gcatttcgat
     6121 aacccaaccg atgccgacac actggtaatt gtgccgccgg aacctgtctc aaccaacgaa
     6181 gggaatatcc tcggacactc gccgcgtaag ctcgggatcg gcatggtgga aattaaagtg
     6241 gtagaacgtg aagggtaatt cctgactgat gtgtaggtcg catagggtgc gacctacacg
     6301 ccttctccgg cttacaacac attattaaga aacaaaaaaa acagcgccca acacttcccc
     6361 ggacgctgca tggcaacttt aataatctcg ccttatcaga aagtaattac acccttaatc
     6421 aactcacgat tgttaatcac atcgcgctcg taggtttctg ccagcgtggc gaacggatag
     6481 cgatgggtta acatcatgtc ggcagtgatt ttcccttccg ccatcagtcg accgacttta
     6541 gcgaaatctt ccggcgtggc gttgcggctg cccatcatcg tcgtttcttt tttatggaat
     6601 tccggatcgg agaactgcaa ctcaccttta aacagaccga caaataccac cgtgccgccg
     6661 tgacgaatca gattcacggt attattcatc gcatgttgat tacctgtcgc gtcgatcact
     6721 ttctgcgcca gcgaaccacc aaactgcgcc cgcagctgcg cgtcaaaatc ttcggctgac
     6781 gggtccagca cgggcaactc cagacgcgtt gccacatgtt cacggcgcgc cggactggta
     6841 tccgccacca ccacctgtgc gccatcggct ttagcgattg ccgccgcgcc cagaccgatt
     6901 ggccccgccc cgaccaccag cacctgctcg ccgggagcaa tggctgcgcg acgcaccgca
     6961 tgagcgctaa tagcgaaagg ttcaatcaat gcggccgcct gcgggtcaat accgtctgcg
     7021 ggcaaaatgt tcgccaccgg cacgctcaaa tactcactaa aaccgccatc ctgatgcacg
     7081 ccaatgactg aaattttttc acagcagttg gtacgcccgc ttttacacgc cgggcattgc
     7141 tgacaggcaa cataagggat cacagcaact tgctgaccat ttttaagatc agcaatattt
     7201 ttacccagcc caacaatctc cccacatatt tcatggccta aaacccgtgg ataactaaaa
     7261 aatggttgat ttccacccta ggcatgaata tcggtaccgc aaatcccgac agacttaatt
     7321 tttattaatg cttcattgtc acccggaata ggtatctcgc gttgtttcca gactaattct
     7381 ttcggctgct ggcaaattaa aacattcatc gtagacatga ttcggctatc cattttttgt
     7441 tgccttagat atcaatgaaa atgaaaaatg gcagcggaac ggaaaatctg ttttgtgaaa
     7501 acacacgcat aaaaatgttt taaatcgggt tttaatggac tcacaaggag gaaaatatga
     7561 gtcgttcaca aaatttacgc cacaatgtga ttaaccaggt cattgatgat atggcgcggg
     7621 gtcatatccc ttccccactt ccatcgcaaa gtgcgctggc ggaaatgtac aacatcagcc
     7681 gcactacagt gcgtcatatt ctcagccaat tacgcgaatg cggcgtctta acgcaggttg
     7741 gaaacgacta tgttattgcg cgtaaacctg accatgatga cggtttcgcc tgtactaccg
     7801 cttcaatgag tgaacaaaac aaagtgtttg agcaggcttt tttcacgatg ataaaccagc
     7861 gtcagttacg ccctagggaa actttttctg aactgcaact ggctcgggcc gcgggtgtca
     7921 gcccggtggt tgtaagagaa tatcttttaa aattcggccg ttataatctt attcaaagcg
     7981 aaaagcgcgg ccagtggagc atgaagcaat tcgatcaatc ctacgccgag cagctgtttg
     8041 agctacgcga aatgctcgaa acacattcac ttcagcactt tctcaacctg cctgatcatg
     8101 atccccgctg gctgcaggcg aaaaccatgc tagaacgtca tcggctactg cgtgacaaca
     8161 tcggcaatag ctttcgcatg ttctcgcaac ttgacagaga ttttcactcc ttactgcttt
     8221 cagctgccga caatatcttt tttgatcaat cgcttgagat catctcagtc atctttcatt
     8281 ttcactatca atgggacgaa agcgatctta aacagcgcaa tatcattgcc gtcgatgaac
     8341 atatgaccat tctcagcgct ctgatttgcc gcagtgacct tgatgccacc cttgcgctcc
     8401 gtaaccattt gaattcagct aaacaatcaa tgattcgatc cattaatgag aacacgcgtt
     8461 atgctcatta agtacggatt aaaaacagca aactattagc gggtgcacgg ttttatcaaa
     8521 agataaatac cgggaaattc ccgcttacct atgctcacaa tcagtcgact aaaaatgttt
     8581 ttgtgagcgg tagtaaagtc ctaaaacttt aacctgccgt taatgtcaac cttgcgtaat
     8641 gcacaattac gtaaacaatt aaaattataa atacaggagt ccggcgtgga aaaagaaaat
     8701 atcaccctcg atccgcgttc ttcatttact ccatcttcgt cggcagatat tcccgtgcca
     8761 ccagatggat tagttcaacg cagtacccga attaaacgca ttcaaaccac cgccatgttg
     8821 ttattatttt ttgcggcggt aatcaattat ctcgaccgca gttcgctgtc ggtagcaaat
     8881 ttaacgattc gtgaagaatt gggattaagt gccaccgaaa tcggcgcttt gctctccgtg
     8941 ttttcactcg cttacggcat tgcgcaactt ccttgcggcc cactattgga tcgtaaaggc
     9001 ccacgcctga tgctgggact ggggatgttc ttctggtcac tgttccaggc catgtctggc
     9061 atggtgcaca gctttacgca gttcgtgttg gtgcgtatcg gtatggggat tggtgaagcg
     9121 ccgatgaacc catgcggtgt aaaagtcatt aacgactggt tcaacatcaa agagcgcgga
     9181 cgcccgatgg gcttcttcaa cgcagcttct accattggcg ttgctgtaag cccaccgatt
     9241 ctggcggcaa tgatgctggt gatgggctgg cgcgggatgt ttattaccat tggtgtactg
     9301 gggatttttc tcgccatcgg ctggtatatg ctctatcgca accgcgagca cgtagaactg
     9361 actgccgttg aacaagctta tctcaatgca ggtagcgtca atgcccgccg agatccgctc
     9421 aattttgccg aatggcgcag cctgttccgt aaccgcacaa tgtggggaat gatgctcgga
     9481 ttcagtggca tcaactacac tgcgtggctg tatctggcct ggcttcctgg ttacctgcaa
     9541 acagcctata acctggattt aaaaagcaca gggttgatgg cggctatccc tttcctgttt
     9601 ggggctgccg ggatgctggt caacggttac gttactgact ggctggtcaa agggggaatg
     9661 gctccgatta aaagccgtaa gatctgcatt attaccggga tgttctgttc tgccgccttt
     9721 acgctgatag taccacaagc gacaacatcc atgatggcgg ttctgctgat tggcatggca
     9781 ctgttctgta ttcactttgc cggaacatcc tgctggggct tgatccacgt cgcagttgct
     9841 tctcgcatga ctgcgtcggt gggcagtatc cagaactttg ccagcttcat ctgcgcctct
     9901 ttcgcgccga tcattactgg ttttattgtt gatacaaccc actcattccg tctggcacta
     9961 atcatttgcg gttgcgtcac cgcagcggga gcactggcgt acatcttcct ggttcgccag
    10021 ccgatcaacg acccgcgtaa agattaaact ccctatgccc gataacattt tgcttatcgg
    10081 gcatttttat ggcgattaaa acgtttccca gttctccccg ctatctgcca ctgccatttt
    10141 acgcggcgta gctggcgtca cggtttttac cacagccgat gtttcacgct gctgttgctg
    10201 ctgttgctga atccggaaca ctgccacagc ttcggtcagg cgactggcct gctcttccag
    10261 cgcggcggcg gcagcggcag attcttccac cagcgcggcg ttctgttgag ttacccggtc
    10321 catctcagca accgctaagc caacctgatc gataccacgg ctctgctcat cagaagcaga
    10381 agctatttcg cccataatgt ccgtcacgcg ggtcacagca ctgacaatct ccgccattgt
    10441 ttccccggcg ctttcgacca gcgtagagcc aacatccact ttccccaccg agtcttcaat
    10501 caggctttta atttcacgaa ccgcctgggc gctacgctgg gccagattac gcacttctcc
    10561 cgcaaccacc gcaaaaccac gtccttgctc acccgcacgc gctgcttcaa ccgccgcgtt
    10621 caaagccaga atattggtct ggaaggtaat gccgtcaatt acgctgataa tatcggcgat
    10681 tttctgcgaa ctggtggaga tatcgcgcat agtctgcacc acgttatcta ccactttgcc
    10741 gccgcgttgc gccgtttcag aagcacttaa tgccagatgg ctggcctggc gcgcattctc
    10801 ggcgttctgt ttcaccgttg cggtcagttg ctccatgctg gctgccgtct cttccagcga
    10861 agcggcctgt tgctcggtgc gcgaagagag atcattattg ccggtagcga tttcgctggc
    10921 accgctatag atggcattgg ccccgttgcg cacatcaccg acggtacgca tcagctctcc
    10981 ctgcatatgg cgcaaactct ctgccagttg ccccatctca ttagagccat ccacctcaat
    11041 cggtttcacc agatcgccgc ctgcaatatg acgaatgctg tcaatcaggc gattcattgg
    11101 cgctaccagc gaggctttaa taccgaacca gacggcgaag atgaccgcca gtacgacgat
    11161 catcacgccc accagaatcc acatcgcctg gctgtaggag gcattgttat cgctgacggc
    11221 gatatcatag agccgatcgt tttgctccat gtaagccaca tactgcttct cgaaaccgtc
    11281 ctgatatccc tgggtcggct gatcaaagaa ctcgttgatt ttgcctgcac ctaacagttg
    11341 gatcagctcc gccagcgcat tgtgataaat atcgtaatta cgtttgatct ctgccgctgc
    11401 ggcggtgctc tgacgcgggt cacgcggcaa cgcttcgtaa tccgcccagt ttttttccgc
    11461 ctgtttcagc gaaatactgg cactctgcat cagctcagca acggttgaac cgctaccaat
    11521 attattctga tccatcatgt agcggatacc cgcgcggttg agggtgttac gcgtctgcaa
    11581 caacgcgacc cagctgccat tcagcgtggg ttgctgctgg cgaatggttt gtaaaacagt
    11641 gaaattttct ttgtcattct ttaaggcatt aaaaaacaga ccgcctgatg tcagttgtaa
    11701 aaggccaaaa acggccaaaa ccagcagtaa gctggtcacg atcttgatac gttttaacat
    11761 ggtttctctt tcctgtggac cgatgcaaga ttttcggcct ggaaagcaaa aactttatga
    11821 aaaggtgtaa cccgccatcc atggaaaaac caggcgctgg ttatcagata ctaaaaagtt
    11881 attcattatc gcccgggatg tcttcttgtc gggaattgcc cagagcaata aattcctcta
    11941 acagatgggt taactgttgc attttctcgg cagtaaactg agcttcaatc tgccgatagg
    12001 cttcttcaat ctgtgtctga gcgcggttat acagcgcctg cccctctttg gtcagcgaga
    12061 tatagagctt ccgctgatca ttgatcggct ttaatcgcaa cactaaaccg tcgcgttcca
    12121 tccgcgtgag gatcccggtc aggcttgggc gcaaaatgca ggcgcgatac gccagatcgt
    12181 gaaaatccat tgatgggctt tccgccagaa tacgcacgat gcgccactgt tgctcagtca
    12241 aattatgccg tttaacgatt gggcgaaaat aactcatcgc cgcttctcgc gcctggagca
    12301 acgcaatggt tagtgagtcg tgcattatct ttcccctgaa aaattcaaaa tcattaatat
    12361 agaaacaatt aatcatcacg ttaccgggac tgagctgccc ccgcaattct tcattttatc
    12421 aaaattgatg atattttatt aacttactga tatttaatat tttattatct ttaaatgtaa
    12481 atagtttgtt aattggatca catttatatc acttactttt gccaaactac gcaatcggga
    12541 ataaaagtaa tcattaacat attaatgatt aagatgatcg aatccgagga gtggtaatga
    12601 aaggcactat cttcgccgta gcgttgaacc atcgcagcca acttgatgcc tggcaggaag
    12661 cgttccagca atctccctac aaagccccgc ctaaaactgc ggtctggttt attaaaccgc
    12721 gcaatacggt gattggttgc ggtgaaccga ttccctttcc acagggtgaa aaagtactga
    12781 gcggtgcgac tgttgcgctg attgtgggga aaacggcgac gaaagtacgt gaagaagatg
    12841 cggcagagta catcgccgga tatgcgctgg ataacgacgt cagcctgccg gaagagagct
    12901 tttaccgtcc ggcaatcaaa gcaaaatgtc gtgatggatt ctgccccatt ggcgaaaccg
    12961 tggctctcag caatgtcgat aatctgacta tctataccga gatcaacggg cgtcctgccg
    13021 atcactggaa taccgccgat ttacaacgta acgccgcaca gctgctgagc gccctgagcg
    13081 aatttgccac gctgaatcca agcgatgcta ttctgctcgg cacgccacag gcgcgcgtgg
    13141 aaatacagcc aagcgatcgc gtgcgtgttc tcgcagaagg tttcccgccg ctggaaaatc
    13201 cggtagtgga cgaacgtgaa gtgaccacgc gcaagagctt cccaacgctg ccacacccgc
    13261 acggtacgct gtttgccctc ggcctgaact acgccgacca cgccagcgaa ctggaattta
    13321 agccaccgga agagccgctg gtgttcctga aagcgccgaa taccctcact ggcgacaacc
    13381 agacctccgt gcgcccgaac aatattgaat acatgcacta cgaagcggag ctggtggtag
    13441 ttattggcaa gcaggcgcgt aacgtcagcg aagccgatgc catggattat gtcgcgggct
    13501 acaccgtgtg taacgactac gccattcgcg actatctgga aaactactac cgccctaacc
    13561 tgcgggtcaa aagccgcgac ggactgacgc cgatgctttc aaccatcgtg ccgaaagagg
    13621 cgatcccgga cccgcataat ctgacccttc gcaccttcgt caacggcgag ttacgccagc
    13681 aaggcaccac cgccgatctg atcttcagcg tgcccttcct gatcgcctac ttaagcgaat
    13741 ttatgaccct gaatccgggc gacatgatcg ccaccggcac accaaaaggc ttatctgacg
    13801 tggtgcctgg cgatgaagta gtggtggaag tagaaggcgt gggccgcctg gtgaaccgaa
    13861 ttgtgagtga ggaaacagcg aaatgaaaaa agtaaatcat tggatcaacg gcaaaaatgt
    13921 tgcaggtaac gactacttcc agactaccaa tccggcaacg ggtgaagtgc tggcggatgt
    13981 ggcctctggc ggtgaagcgg agatcaatca ggcggtagcg gcagcgaaag aggcgttccc
    14041 gaaatgggct gatctgccga tgaaagagcg tgcgcgcctg atgcgccgtc tgggcgatct
    14101 gatcgaccag aacgtgcctg agatcgccgc gatggaaacc gcggacacgg gcctgccgat
    14161 ccatcagacc aaaaatgtgt tgatcccacg cgcttctcac aactttgaat ttttcgcgga
    14221 agtctgccag cagatgaacg gcaagaccta tccggttgac gacaagatgc tcaactacac
    14281 gctggtgcaa ccggtaggcg tttgtgcgct ggcgtcgccg tggaacgtgc cgtttatgac
    14341 cgccacctgg aaggtcgcgc cgtgtctggc gctgggcaat accgcggtgc tgaagatgtc
    14401 ggaactctcc ccgctgaccg ccgaccgcct gggtgagctg gcgctggaag ctggtattcc
    14461 ggcgggcgtg ctaaacgtgg tacagggcta cggcgcaacc gcaggcgatg cgctggtccg
    14521 tcatcatgac gtgcgtgccg tgtcgttcac cggcggtacc gccaccgggc gcaacatcat
    14581 gaaaaacgcc gggctgaaaa aatactccat ggagctgggc ggtaaatcac cggtgctgat
    14641 ttttgaagat gccgatattg agcgtgcgct ggacgccgcc ctgttcacca tcttctcaat
    14701 caacggcgaa cgctgcaccg ccggttcgcg catctttatt cagcagagca tctatccaga
    14761 attcgttaaa cgctttgccg aacgcgccaa ccgtctgcgc gtgggcgatc cgaacgatcc
    14821 gaatacccag gttagggcgc ttatcagtca gcaacactgg gaaaaagtct ccggctatat
    14881 ccgtctcggc attgaagaag gcgcaaccct gttggcgggc ggcccggata aaccgtccga
    14941 cctgcctgcg cacctgaaag gcggcaactt cctgcgccca acggtgctgg cggacgtaga
    15001 taaccgtatg cgcgttgccc aggaagagat tttcgggccg gtcgcctgcc tgctgccgtt
    15061 taaagacgaa gccgaaggct tacgcctggc aaacgacgtg gagtacggcc tcgcgtcgta
    15121 catctggaca caggatgtca gcaaagtgtt acgcctggcg cgtggcattg aagcaggcat
    15181 ggtgttcgtc aacacccaga acgtgcgcga cctgcgccag ccatttggcg gcgtaaaagc
    15241 ctccggcacc gggcgtgaag gcggtgagta cagcttcgaa gtgttcgcgg aaatgaagaa
    15301 cgtctgcatt tccatgggcg accatccaat tccgaaatgg ggagtctgat atgggtaagt
    15361 tagcgttagc cgccaagatc acgcacgtac cgtcgatgta tctctctgaa ctaccaggaa
    15421 aaaaccacgg ttgtcgccag ggtgcgatcg acgggcataa agagatcagc aagcgttgcc
    15481 gggaaatggg cgtcgatacc attatcgttt tcgatactca ctggctggtc aacagtgctt
    15541 atcacatcaa ctgtgcagac cattttgaag gcgtctacac cagcaacgag ctgccgcatt
    15601 ttattcgcga catgacctac aactacgagg gcaacccgga gttggggcag ctaattgccg
    15661 aggaagcctt aaagctcggc gtgcgggcaa aagcgcacaa tattcccagc ctgaaactgg
    15721 aatacggcac gctggtaccg atgcgctaca tgaatgaaga caagcacttc aaagtggtct
    15781 ccatttcggc tttctgcacg gttcacgatt ttgccgacag ccgcaagctg ggcgaagcga
    15841 ttctgaaagc gatcgaacag tacgacggca ccgtggcggt ccttgccagc ggttcgttat
    15901 cgcaccgctt tattgacgat cagcgtgcgg aagaagggat gaacagctac acccgcgagt
    15961 tcgaccgcca gatggacgag cgtgtggtga agctgtggcg cgaaggccag ttcaaagagt
    16021 tttgcaatat gctgccggag tacgccgact actgctacgg cgaaggcaat atgcacgaca
    16081 cggtgatgct gctggggatg ctcggctggg ataaatacga cggcaaggtg gagtttatta
    16141 ccgagctgtt cccaagctct ggcaccggtc aggttaacgc tgttttcccg ctgcccgcgt
    16201 aaggacgttt tatgccgcac tttatcgtgg aatgtactga aaatattcgt gaagaagccc
    16261 gcttacctga gctgtttgcc agtgttaaca ctgcactggc ggcaacgggg atttttccgc
    16321 ttgggggaat acgtagccgg gcgcactgga tcgatacctg gcaaatggct gatggcaagc
    16381 acgattacgc ctttgtgcat atgacgctaa aaatcggcag cggacgcagt ctggaaagcc
    16441 gtcaggaagt gggcgaaatg ctgtttgatc tgattaaaac gcacttcgcc tcactgatgg
    16501 agagccgtta cctagcgctt tcatttgaga ttgcagagct gcacccgacg ttgaatttca
    16561 aacagaacaa cgtacacgcg ttgtttaaat aaatttgcac cagcttccag gccggatgat
    16621 aaggcgctgc cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac
    16681 accctgatcg cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc
    16741 tcgctggatt acccggagat caccatcgaa gacgcctacg ccgtgcagcg cgaatgggtt
    16801 cgcctgaaaa tcgccgaaag tcgcacgctg aaaggccaca aaatcggtct gacctcgaaa
    16861 gcgatgcagg caagctcgca gatcagcgaa ccggattacg gtgcactgct ggacgacatg
    16921 ttcttccacg atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg
    16981 gagctggctt ttgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtt
    17041 tacaacgcca cggactatgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac
    17101 atcgatccgg aaacccagcg cccgcgcaaa gtattcgaca ccatttccga taacgccgcc
    17161 aacgccggag tgatcctcgg aggtcgtccg atcaaaccag acgaactgga tctgcgttgg
    17221 atctccgccc tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggtgtg
    17281 ctgaatcatc cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta
    17341 caactggaaa ccgggcaaat cattctcggt ggttcgttca ccctcccggt tccggcgcgt
    17401 aagggcgaca ccttccacgt cgattacggc aacatgggct ccattagctg ccgctttgtt
    17461 taaggagaga acgatggaaa acagttttaa agcggcgctg aaagcaggcc gtccgcagat
    17521 tggattatgg ctggggctga gcagcagcta cagcgcggag ttactggccg gaccaggatt
    17581 cgactggtta ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca
    17641 actacaggcg attgcgccct accccagcca gccagtggta cgcccatcgt ggaacgatcc
    17701 ggtgcaaatc aaacaactgc tggacgtagg cacacaaacc ttactggtgc cgatggtaca
    17761 aaacgccgac gaagcccgta aagcggtacg cgccacccgt tatccccccg ccggtattcg
    17821 cggtgtgggc agtgcgctgg ctcgcgcctc gcgctggaat cgcattcctg attacctgca
    17881 aaaagccaac gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa
    17941 cttaccgcag attctggacg tggaaggggt cgacggcgtg tttatcggcc cggcggatct
    18001 gagcgccgat atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga
    18061 gcaggcgatc gtgcagatcc gcgaagcggg caaagcgccg gggatcctga tcgccaatga
    18121 gcaactggca aaacgctatc tggaactggg cgcgctgttt gtcgccgtcg gcgttgacac
    18181 caccctgctc gcccgcgccg ccgaagcgct ggcagcacgg tttggtaata gttcttctat
    18241 ttcgatggcg aagcaaaaca acaactctgt ctactaaacg tacgatgtgg agcgcatcat
    18301 gacgacctct accctgcaat ataaagataa taaaactgtt gaaattgaac atcgtgtcat
    18361 taataaatta tttcgtcgcc taatcatttt tctttttatt ctgtttgttt tttcgtttct
    18421 tgatcgcatc aatataggat ttgccggact gacaatgggc aaggatcttg gcctgacgtc
    18481 cacaatgttt ggcctcgccg caacactctt ttatgtcacg tacgtcatgt gcgggatccc
    18541 cagtaatatg atgttagcga aaattggcgc tcgtcgatgg atcgctggga tcatggtggt
    18601 ctggggaatt gcttccacct gcacaatgtt tgcaacaagc cccgaaacac tctatctact
    18661 gcgtatgctg gttggtattg ctgaggcggg ttttttacct ggtattttgg tttatctgac
    18721 ctggtggttc ccggcatatc atcgggccag agccaatgcc ttatttatga tagcgatgcc
    18781 agtcactatg atgctgggat tgatcctttc aggctacatt ctggccttgg atggactgtg
    18841 gaatctaaaa ggctggcaat ggctcttttt gctggaaggt ctgccatcgg tcattcttgg
    18901 tgtcgtcacc tggttttatc tcaacgacac accagaccag gcgacctggc ttgatgaaaa
    18961 cgaaaaacag gctttaaaaa cgatgattct ccgtgaacaa gagagtgcat ttgcccatgc
    19021 atctgttcca cgctcaacct tacgtgaagt cctgacccca gccatcatgc tatatacact
    19081 tgcttatttt tgcctgacta atactctgag tgcgattaat atctggacgc cgcaaatcct
    19141 acagagtttc aatactggca gtagcaacat catgattggt ttgctggcgg ccatccccca
    19201 attctgcaca atttttggca tgatctggtg gagtcgtcgt tccgaccgtc ttaaagaacg
    19261 caaaaagcat acgattcttc cctacctgtt tgccgctgcc ggatggttac tcgcttctgc
    19321 gacagatcac agcctcattc aactgagcag catcattatg gcctcaatgg gatcatttac
    19381 cgcgatgact atcttttgga caacaccgga tcaagttata agcttgcaat cgcgtgccgt
    19441 tgcgatggct gtcatcaatg ctgtcggcaa tgtaggttca gcagtcagtc cattacttat
    19501 cggtattttg aaagatgcaa ccggcagttt tagctcaggt ttgtggtttg tagcaggatt
    19561 gctggtactg ggcgcgttag tcatgacccg aatccctatg gggagtcggg aacactctct
    19621 ttcatctgaa cttatcgcac caaaaccgca ataaggagtc atgatgtcca gtgaagctat
    19681 ctttgaacat ggttatgaaa atattgattt ggtccgggaa tatgatgccc gctacgctgg
    19741 tgaagatgtg cattatgaaa catttgcccg gttagcgaat ttctttggac gcgatatgcg
    19801 ccctcactgg cacgatagat attttcaact ccattatctg gtcactggac gcattacttt
    19861 acaactggat gagcacttct attctgtgcg cgcaccgctt tttgtactaa caccaccatc
    19921 ggtgccacat actttcttta ctgatcctga tagcgatggg catgttctga ccgtacgtca
    19981 ggagttaatt tggcccctgg tagaaaagct ctggcctggt agtggagatg ccgcaatgct
    20041 acaaggtatc tgtctgtcac tggagggaat ggaacataca ctggcggcat taaatcatta
    20101 ttggccgata atatccctgg agtttcaaca aaataagcaa ggacgggaaa ttctattgca
    20161 atcactggcg caaagtattt ttacaatcct actgcgtgaa gcaccaccaa atgatatttc
    20221 aacatgcagc gtgcgcggcg agatgtgttt attccagaag tttaatcggc tgattgacga
    20281 aaattatcgc caacatttaa tggtccctga atatgcaaca atgctcggta tgagtgaatc
    20341 acggcttacc gagctgtgcc gacgttttgc caatcaatcg ccgaagagat tgattttcga
    20401 acgagtgagc cgggaagcca gacggcttct gctctatagc gagcaaagca tcaaccagat
    20461 agcccttgat cttggctaca aagatcccgc ctatttcgcc cgttttttca accgaatgat
    20521 gggttgctca ccaacacaat tccgtggtcg ctgaccattt atcaacctga gagactcgac
    20581 atgaaaatat ctttacgctc actatttgtg gcgtgcctga ttgcgccagt ggcattcacc
    20641 tacgcctccg aaccgcaata aatgaaaatt gtactacttg gaacgaaagg tggcccttct
    20701 ttattaaaag ctgaccgttt accgatggca acggcactta ttattggcaa taaaacctgg
    20761 ttaattgatg ctggttacgg ggccagctta caactggtaa aaaataatat tccactgcgc
    20821 cagattgata atattttact cacccattta cacagtgatc atattctgga ctatccatca
    20881 gtcttaatga atgcatgggc tagcggtctg caggacaaaa ctattcatgt attcggtccg
    20941 ccaggcaccc gagccatgac cgctgccagt tggcaggtct tcgatcggga catcacactg
    21001 cgtatggaag aagaaggaaa acccgatcct cgcaaactcg ttacagctca agatgttggt
    21061 gaaggcgtag tttataaaga tgataccgtg accgtgactg cattaaaggt ccctcattct
    21121 cctttccctg acggtgaggc ttttgcttat cgcttcgaca cacaaggcaa gcgaattgtc
    21181 ttctcaggag atacctcgta ctttccccca ctggcaactt tctcagaagg cgctgatata
    21241 cttgtgcatg aggccgtaca tgagccctcc gtcgctaagc tggcagacag tattggcaat
    21301 ggtaaaacac tggcaaaagc cattgcttct caccatacga ctattgagga tgtaggcaaa
    21361 attgccaatg aagcaaaagt gaaaaaactc atccttagcc acctggtacc aatcaccgtt
    21421 ccggatgaaa catggaagca agaagcacag aaaactattc aggtcctatc gtcgttggtc
    21481 atgaaaatat gacgattacc attccattcg ccccataaaa aattccgtcc ccgttagccc
    21541 ttgttagcgg ggaatattat gatttgcctt actgtaagtt gatcacgatc gcagtttact
    21601 atttaactcc accttgctgt cgaaaagtac caacgttcgc ctgaaacgtc tcttcccgac
    21661 aaccgcgttc aacgcttcaa ttaaataaca aaaacaacac acaaaattaa caatcgtatt
    21721 ccgattaata ctgtagaggt cgacatgaaa ccagaagatt tccgcgccag tacccaacgt
    21781 cctttcaccg gggaagagta tctgaaaagc ctgcaggatg gtcgcgagat ctatatctat
    21841 ggcgagcgag tgaaagacgt caccactcat ccggcatttc gtaatgcggc agcgtctgtt
    21901 gcccaactgt acgacgcgct gcacaaaccg gagatgcagg actctctgtg ttggaacacc
    21961 gacaccggca gcggcggcta tacccataaa ttcttccgcg tggcgaaaag tgccgacgac
    22021 ctgcgccagc aacgcgacgc catcgctgaa tggtcacgcc tgagctatgg ctggatgggc
    22081 cgtaccccag actacaaagc cgctttcggt tgcgcactgg gcgcgaatcc gggcttttac
    22141 ggtcagttcg agcagaacgc ccgtaactgg tatacccgta ttcaggaaac tggcctctac
    22201 tttaaccacg cgattgttaa cccaccgatc gtcatttgcc gaccgataaa gtgaaagacg
    22261 tttacatcaa gctggaaaaa gagactgacg ccgggattat cgtcagcggt gcgaaagtgg
    22321 ttgccaccaa ctcggcgctg actcactaca acatgattgg cttcggctcg gcacaagtga
    22381 tgggcgaaaa cccggacttc gcactgatgt tcgttgcgcc aatggatgcc gatggcgtga
    22441 aattaatctc ccgcgcctct tatgagatgg tcgcgggtgc taccggctcg ccatacgact
    22501 acccgctctc cagccgcttc gatgagaacg atgcgattct ggtgatggat aacgtactga
    22561 tcccatggga aaacgtgctg atctaccgcg attttgatcg ctgccgtcgc tggacgatgg
    22621 aaggcggttt cgcccgtatg tatccgctgc aagcctgtgt gcgcctggca gtgaaactcg
    22681 acttcattac ggcactgctg aaaaaatcac tcgaatgtac cggcaccctg gagttccgtg
    22741 gtgtgcaggc cgatctcggt gaagtggtgg cgtggcgcaa caccttctgg gcattgagtg
    22801 actcgatgtg ttcagaagcg acgccgtggg tcaacggggc ttatttaccg gatcatgccg
    22861 cactgcaaac ctatcgcgta ctggcaccaa tggcctacgc gaagatcaaa aacattatag
    22921 aacgcaacgt taccagtggc ctgatctacc tcccttccag tgcccgtgac ctgaacaatc
    22981 cgcagatcga ccagtatctg gcgaagtatg tgcgcggttc gaacggtatg gatcacgtcc
    23041 agcgcatcaa gatcctcaaa ctgatgtggg acgccattgg cagcgaattt ggtggtcgtc
    23101 acgagctgta tgaaatcaac tactccggta gccaggatga gattcgcctg caatgtctgc
    23161 gccaggcaca aagctccggc aatatggaca agatgatggc gatggttgat cgctgcctgt
    23221 cggaatacga ccagaacggc tggactgtgc cgcacctgca caacaacgac gatatcaaca
    23281 tgctggataa gctgctgaaa taacgcagca ggaggttaag atgcaattag atgaacaacg
    23341 cctgcgcttt catgacgcga tggccagcct gtcggcagcg gtaaatatta tcaccaccga
    23401 gggcgacgcc ggacaatgcg ggattacggc aacggctgtc tgctcggtca cggatacacc
    23461 accgtcgctg atggtgtgca ttaacgccaa cagtgcgatg aacccggttt ttcagggcaa
    23521 cggcaagttg tgcgtcaacg tcctcaacca tgagcaggaa ctgatggcgc gccacttcgc
    23581 gggcatgaca ggcatggcga tggaagagcg ttttagcctc tcatgctggc aaaaaggtcc
    23641 gctggcgcag ccggtgctaa aaggttcgct ggccagtctt gaaggagaga tccgcgatgt
    23701 gcaggcgatt ggcacacatc tggtgtatct ggtggagatt aaaaacatca tcctcagtgc
    23761 agaaggtcac ggacttattt actttaaacg ccgtttccat ccggtgatgc tggaaatgga
    23821 agctgcgatt taagctccag ccccttcacg cattcgtcga aggggcattt ttattgtgat
    23881 ctcaatcaca tatctacccg atgaataatt cttgcccaac cgctcaccgc cattcctgac
    23941 cgcttacatc cctaaaataa ccactcagtt atttacctta ctttacgcgc acgtatctct
    24001 ggcaacatca ctgcaggata gcggtcaatt tacctcctca aacgcaacgc aaacctagaa
    24061 cggcttcggc ctatgattaa caaattactc tatggatttt ggtttgcagg aaggcagcaa
    24121 gtgagtgaat ccccgggagc ttacaaaagt aagtgtcaga ggtgaacgaa cgcagctgcc
    24181 gcacctgtaa gccaaaagac gacgagtaaa atgccaggtt ttactatgga tacgaaaaaa
    24241 ctattcaagc acataccctg ggtgattctc ggaatcatcg gtgcattctg cctcgcggta
    24301 gttgcattac gtcgggggga gcacgtcagc gccctgtgga tcgtggtcgc ctctgtgtcg
    24361 gtgtatctgg tggcgtatcg ctactacagt ctgtacatcg cccagaaggt gatgaaactc
    24421 gaccccacgc gcgcgacgcc tgcggttatt aacaacgacg gtctgaacta cgttccgacc
    24481 aaccgttacg tgttgtttgg tcaccacttc gccgctatcg ccggtgctgg tccgctggtg
    24541 ggaccggttc tcgccgcgca gatgggctac ctgcctggca cgctgtggct gctggcgggg
    24601 gtagtgctgg ccggtgcggt acaggacttt atggtgctgt ttatctcctc tcgccgtaac
    24661 ggcgcatctc tcggtgagat gatcaaagaa gagatggggc cagtaccggg gactatcgcg
    24721 ctgtttggct gtttcttaat catgatcatc atcctcgccg ttctggcgct gattgtggtt
    24781 aaagccctgg ccgaaagtcc gtggggtgtc ttcaccgttt gctcaaccgt accgattgcg
    24841 ctgtttatgg gtatctacat gcgctttatc cgtccggggc gtgtgggtga agtctctgtt
    24901 attggtatcg tgctgctggt tgcctctatc tacttcggtg gcgtgattgc tcacgatccg
    24961 tactggggtc cggcactgac ctttaaagac accaccatta ccttcgcgct gattggctat
    25021 gcgtttgttt ccgcactgct gccggtgtgg ctgatcctcg caccgcgcga ctatctggca
    25081 accttcctga aaatcggcgt tatcgtcggc ctggcgctgg gtatcgtggt gctgaacccg
    25141 gaactgaaaa tgcctgcgat gacccagtac attgacggta ctggcccgct gtggaaaggc
    25201 gctctgttcc cgttcctgtt catcaccatc gcctgtggtg cggtatctgg cttccacgcg
    25261 ctgatctctt ccggtacgac gccgaaactg ctggctaacg aaaccgacgc gcgtttcatt
    25321 ggctacggcg caatgctgat ggagtccttc gtggcgatta tggcgctggt tgcagcgtcc
    25381 atcatcgaac cgggtcttta cttcgcgatg aacaccccgc ctgctggcct tggcatcacc
    25441 atgcctaacc tgcatgaaat gggtggcgag aacgcgccga tcatcatggc gcagctgaaa
    25501 gacgttaccg cacacgcggc agcgaccgtc agctcctggg gcttcgtgat ttcgccagag
    25561 cagatcctgc aaaccgcgaa agacattggt gagccttctg tcctgaaccg tgcaggtggt
    25621 gcgccgacgc tggcggtagg tatcgctcac gtgttccaca aagtgctgcc gatggctgac
    25681 atgggcttct ggtatcactt cggtattctg ttcgaagccc tgttcatcct gaccgcgctg
    25741 gatgcgggta cccgttctgg ccgctttatg ctgcaagacc tgctgggtaa cttcatcccg
    25801 ttcctgaaaa aaaccgactc tctggttgcc ggtatcatcg gtactgcggg ctgtgtgggt
    25861 ctgtggggct acctgctgta tcagggcgtg gtcgatccgc tgggcggcgt taagagcctg
    25921 tggccgctgt tcggtatctc caaccagatg ctggcagccg tagcgctggt actgggcacc
    25981 gttgtgctga ttaagatgaa gcgcacccaa tacatctggg taactgttgt tccggctgta
    26041 tggctgctta tctgcaccac ctgggcgctg ggtctgaaac tgttcagcac caacccgcag
    26101 atggaaggct tcttctacat ggcaagccag tacaaagaga agattgctaa cggtactgac
    26161 ctgacggcgc agcagattgc caacatgaac cacatcgttg tgaacaacta caccaacgca
    26221 ggcctgagta ttctgttcct gattgtggtg tacagcatca tcttctacgg tttcaaaacc
    26281 tggcttgcgg tgcgtaacag cgacaaacgt actgacaaag aaacaccgta cgttccaatc
    26341 ccggaaggcg gcgtgaagat ctcttcgcac cactaaccgt gtttagcccc gcttcggcgg
    26401 ggctttgttc ttatcagagt gaactatgtt tggtaactta ggacaggcaa aaaaatatct
    26461 cggccaggcg gcgaagatgt tgattggtat tccagactac gacaactacg tcgagcatat
    26521 gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc gcgaacgcca
    26581 gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat ggagagagca
    26641 tgaacccgat tgcagttacc ctactcaccg gttttttagg cgcgggcaaa actaccctgc
    26701 tgcgccatat tcttaacgaa caacacggct acaagattgc cgtgattgaa aacgaattcg
    26761 gcgaagtctc cgttgatgac caactgattg gcgaccgcgc cacgcagatc aaaacgctga
    26821 ccaacggctg catctgctgt tcgcgctcca acgagctgga ggacgcacta ctcgacctgc
    26881 tggataatct cgacaagggc aatattcagt tcgatcgtct ggtgattgaa tgcaccggca
    26941 tggccgatcc cggcccgatt attcagacct ttttctccca tgagatttta tgccagctct
    27001 acctgctgga cggcgtgatt gcgctggtgg atgcggtaca tgccgatgag cagatgaacc
    27061 agttcaccat cgcccagtcc caggtgggct acgccgaccg tattctgctg actaaaaccg
    27121 acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc gcgcatcaac gcccgcgcac
    27181 cggtctacac cgtcacccac ggcgacatcg acctgggcct gctgttcaac accaacggtt
    27241 ttatgctgga agagaacgtc gtcagcacca aaccgcgttt ccactttatc gcggataaac
    27301 aaaacgatat ttcgtcaatt gtggtggaac tggattaccc ggtagatatc agcgaagttt
    27361 cccgcgtgat ggaaaacctg ctgctggaat cggcggataa actgctgcgt tacaaaggga
    27421 tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag cgcctctaca
    27481 gcgccgactg ggacaggccg tggggcgatg aaaaaccgca tagcacaatg gtgtttatcg
    27541 ggattcagtt gccggaggaa gagattcggg cggcgtttgc gggattgagg aagtaagttc
    27601 gggaaagatc aactttttga gagttccacc gcaacattat tacgagtgga actctttaga
    27661 tatgtcagac caaccaaagc ttcttcaatt gcgttttcat tttttcatgt ctcattttca
    27721 gttcagccaa tccccattct gaagaattac aaacatcttt tgttaaataa aaagagcttt
    27781 tactattaag tttctcataa atagatttct ttttgataaa atcatagtgt tgtgtttcag
    27841 aatttttgga accacttatg agtgttaaat tacctatttt atgcagccag tagacatgct
    27901 cttgaggttg gaatctatta atccagtatt cattaactaa tgcctgagga agaatatgct
    27961 caattgtgat tctccccgta taggttttaa tcacgctctc atcttgttgc tccatatcta
    28021 agcgaagcaa aattgctttt attaagttca cttgattcgg acgaggctca tataaatctt
    28081 catccagagc agctataaaa cctgaattat ctgcatgttg gtttatctga tttatcacag
    28141 aatcaaaagg catatcatta tttattgcta ccagagcaga ataacaaacc atttctcttt
    28201 ggcttttaat ttgttttttt aaccacccat gcatatatac cttctcaaag gcggtaataa
    28261 attgagaaaa atcatctaaa ttaaaatctt ctgtacgagc cattcgattc atgaatgcca
    28321 taaccggagg aatccactca tctaccccta aattacttag cgatgctatt tttcgtcgta
    28381 ttgaagggtg ttcaaaatca ttctctaaaa tcttagtata atttt
//
LOCUS       DAJTRD010000049        28364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTRD010000049 DAJTRD010000000
VERSION     DAJTRD010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.049"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO315_002303"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO315_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1023..2045)
                     /locus_tag="OO315_002304"
     CDS             complement(1023..2045)
                     /locus_tag="OO315_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5891192.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /locus_tag="OO315_002305"
     CDS             complement(2059..3561)
                     /locus_tag="OO315_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5891193.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO315_002306"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO315_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="OO315_002307"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="OO315_002307"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5891194.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="OO315_002308"
     CDS             complement(5877..6218)
                     /locus_tag="OO315_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5891195.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO315_002309"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO315_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5891196.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO315_002310"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO315_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5891197.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="OO315_002311"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="OO315_002311"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5891198.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="OO315_002312"
     CDS             12897..14240
                     /locus_tag="OO315_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5891199.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="OO315_002313"
     CDS             complement(14302..14508)
                     /locus_tag="OO315_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5891200.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="OO315_002314"
     CDS             14833..15387
                     /locus_tag="OO315_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5891201.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="OO315_002315"
     CDS             complement(15450..16388)
                     /locus_tag="OO315_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891202.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO315_002316"
     CDS             complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO315_002316"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5891203.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17530..19473
                     /gene="cpdB"
                     /locus_tag="OO315_002317"
     CDS             17530..19473
                     /gene="cpdB"
                     /locus_tag="OO315_002317"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5891204.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19599..19979)
                     /locus_tag="OO315_002318"
     CDS             complement(19599..19979)
                     /locus_tag="OO315_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5891205.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20068..20928
                     /gene="qorB"
                     /locus_tag="OO315_002319"
     CDS             20068..20928
                     /gene="qorB"
                     /locus_tag="OO315_002319"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5891206.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21037..22002
                     /locus_tag="OO315_002320"
     CDS             21037..22002
                     /locus_tag="OO315_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5891207.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22110..22772
                     /gene="ytfE"
                     /locus_tag="OO315_002321"
     CDS             22110..22772
                     /gene="ytfE"
                     /locus_tag="OO315_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5891208.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO315_002322"
                     /gene_synonym="dagA"
     CDS             complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO315_002322"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5891209.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO315_002323"
     CDS             complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO315_002323"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5891210.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25376..25921
                     /locus_tag="OO315_002324"
     CDS             25376..25921
                     /locus_tag="OO315_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5891211.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25905..26552)
                     /locus_tag="OO315_002325"
     CDS             complement(25905..26552)
                     /locus_tag="OO315_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5891212.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26764..27555
                     /locus_tag="OO315_002326"
     CDS             26764..27555
                     /locus_tag="OO315_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5891213.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27565..>28364
                     /locus_tag="OO315_002327"
     CDS             27565..>28364
                     /locus_tag="OO315_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5891214.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLMPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDIN"
BASE COUNT         6960 a         7550 c         6992 g         6862 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tataaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt
    16441 atagaagtaa actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt
    16501 tttgcacttt aaatacaatc tctttttatt tataactttt taggttggga taaatcagga
    16561 tggcagaatc aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa
    16621 ccccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg
    16681 aacgtgcgct ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt
    16741 tggtccgaag cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga
    16801 agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc
    16861 atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag
    16921 cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt
    16981 cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt
    17041 aacggtgaat tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag
    17101 ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac
    17161 cggaatatcc ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc
    17221 cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc
    17281 gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat
    17341 ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta
    17401 atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc
    17461 actgttctat aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg
    17521 atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg
    17581 aatgcagcga cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg
    17641 gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc
    17701 ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg
    17761 attcagggga gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt
    17821 catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat
    17881 gaatttaact acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat
    17941 gtaaatgcca acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt
    18001 aaagataccg aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt
    18061 ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg
    18121 gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc
    18181 gatgtcattg tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg
    18241 gaaaactcag tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat
    18301 gctcacgccg ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa
    18361 ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg
    18421 gtcgacttac aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa
    18481 gcacggccga tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg
    18541 gtagaaacac tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt
    18601 aaatctgccg acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa
    18661 gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg
    18721 gcaaaactgc cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac
    18781 ccggcaagct atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt
    18841 tatctctacc ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg
    18901 ctggagtgct ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca
    18961 ctcatcaact gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat
    19021 cagattgatg ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat
    19081 gcagagcgga ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc
    19141 ctggttgcca ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc
    19201 catatcgctt ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat
    19261 gagtcgaaac gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg
    19321 atagctgccg ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca
    19381 gcgtttatta aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg
    19441 tttgcgattt atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca
    19501 accgcatccg gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc
    19561 atgaaccgta gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc
    19621 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    19681 ctcacctgct cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac
    19741 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    19801 tcactcaccc caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    19861 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    19921 cactgttccg caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt
    19981 tcatacttac ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    20041 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    20101 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    20161 gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct
    20221 gactacagcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    20281 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    20341 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    20401 ctcggcctcg ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt
    20461 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    20521 ctggaacacg gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg
    20581 gcagattatg cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    20641 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    20701 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    20761 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    20821 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg
    20881 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    20941 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    21001 tgaacgtttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    21061 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac
    21121 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt
    21181 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    21241 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    21301 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt
    21361 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    21421 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    21481 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    21541 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    21601 ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat
    21661 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    21721 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    21781 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    21841 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    21901 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca
    21961 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta
    22021 aataagcctc tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat
    22081 cttatcttat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    22141 tggcgctctc tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct
    22201 gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    22261 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    22321 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc
    22381 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    22441 tgccaaaagg gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga
    22501 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    22561 ggccaatcag cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca
    22621 aacacactac caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt
    22681 ataacggcat taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg
    22741 tactgttccc acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc
    22801 cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc
    22861 agcccaaccc cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat
    22921 cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca
    22981 gcttgccgag cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt
    23041 acaccaggta agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg
    23101 ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc
    23161 cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt
    23221 tagcgaacgc tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag
    23281 agaagacacc gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc
    23341 tggcagccgc aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga
    23401 ctaccgaact ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa
    23461 tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt
    23521 ccgcagctgt tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc
    23581 cggcaaagaa gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga
    23641 atgacgcttc cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga
    23701 caatcagcga cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga
    23761 acattttcac ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga
    23821 cccagtcgga gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg
    23881 ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg
    23941 gccccagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca
    24001 attcccccat tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa
    24061 tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa
    24121 tggcacctcc aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact
    24181 gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc
    24241 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    24301 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    24361 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    24421 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    24481 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    24541 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct
    24601 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    24661 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    24721 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    24781 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    24841 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    24901 ttcacgcctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    24961 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    25021 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    25081 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    25141 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    25201 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    25261 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    25321 gttagcttat tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc
    25381 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    25441 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    25501 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga
    25561 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac
    25621 ccaaacgcaa ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt
    25681 cgaacccggt aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt
    25741 ctatgcaatg gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca
    25801 gatggtgaaa atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg
    25861 gaataatcaa caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta
    25921 gatgaagggt caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct
    25981 tccagtgcct ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg
    26041 atcaaataat atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct
    26101 ggcttaaaaa tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga
    26161 gcatggttaa tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt
    26221 tcactatgca caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca
    26281 ataattttct caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt
    26341 tgtcgaatat cgttccaggt atattccagc acttcgataa ataaattttc tttattacca
    26401 aagtggtgat aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg
    26461 gccccttcat aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc
    26521 aggctctgta cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg
    26581 actatcataa cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat
    26641 caggttatca aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt
    26701 ttatcctcca gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat
    26761 tgtatgaaat ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag
    26821 ggctgtgcgc aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat
    26881 gccgacagcg tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa
    26941 gtgatttgct tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg
    27001 gcgcaaaacc agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa
    27061 aaacgcggtg acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac
    27121 acccgcgcgc cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa
    27181 ccggaagcgg agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg
    27241 ctggcgatga atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt
    27301 ctgtttgctg ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc
    27361 atcaaaactg atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt
    27421 ctggtgccgt ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg
    27481 gcggaaggta aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt
    27541 atgccgcgct tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt
    27601 atcaataccg cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc
    27661 gcacgcggta ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag
    27721 caaatcaccc gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact
    27781 tactacaccg cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg
    27841 ctggacgacg cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca
    27901 acgggcacta aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg
    27961 ctgccacatt cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca
    28021 gctgccgatc gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc
    28081 gatcccgatg gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat
    28141 ccggatctcg ccagtcagat cctgcgtgcc ggaaaacgca tcctcgcgtt tcatgtttcc
    28201 gactggttaa tgccgaccac tgatctggtc aatgaccgtg gtatgccggg agatggcgtc
    28261 atcaatattc cgtcaattcg tcggctggtt gaaaacgccg gatttaatgg tgctattgaa
    28321 ctggaaatat tctcgcctta ctggtggcag aaagatatta acag
//
LOCUS       DAJTRD010000050        28033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTRD010000050 DAJTRD010000000
VERSION     DAJTRD010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.050"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO315_002328"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO315_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5891215.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="OO315_002329"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="OO315_002329"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5891216.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="OO315_002330"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="OO315_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5891217.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="OO315_002331"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="OO315_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5891218.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="OO315_002332"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="OO315_002332"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5891219.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="OO315_002333"
     CDS             6426..7055
                     /locus_tag="OO315_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5891220.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO315_002334"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO315_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5891221.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO315_002335"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO315_002335"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5891222.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="OO315_002336"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="OO315_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5891223.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="OO315_002337"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="OO315_002337"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5891224.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14055..14816
                     /gene="adiY"
                     /locus_tag="OO315_002338"
     CDS             14055..14816
                     /gene="adiY"
                     /locus_tag="OO315_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5891225.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14953..16290
                     /gene="adiC"
                     /locus_tag="OO315_002339"
     CDS             14953..16290
                     /gene="adiC"
                     /locus_tag="OO315_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5891226.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16394..18037
                     /gene="eptA"
                     /locus_tag="OO315_002340"
     CDS             16394..18037
                     /gene="eptA"
                     /locus_tag="OO315_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5891227.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18034..18702
                     /gene="basR"
                     /locus_tag="OO315_002341"
     CDS             18034..18702
                     /gene="basR"
                     /locus_tag="OO315_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5891228.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18703..19803
                     /gene="pmrB"
                     /locus_tag="OO315_002342"
     CDS             18703..19803
                     /gene="pmrB"
                     /locus_tag="OO315_002342"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5891229.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OO315_002343"
     CDS             complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OO315_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5891230.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OO315_002344"
     CDS             complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OO315_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5891231.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21746..22624)
                     /locus_tag="OO315_002345"
     CDS             complement(21746..22624)
                     /locus_tag="OO315_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5891232.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OO315_002346"
     CDS             complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OO315_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5891233.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25252..25587
                     /locus_tag="OO315_002347"
     CDS             25252..25587
                     /locus_tag="OO315_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5891234.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25745..26188
                     /gene="yjdN"
                     /locus_tag="OO315_002348"
     CDS             25745..26188
                     /gene="yjdN"
                     /locus_tag="OO315_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5891235.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26321..27109
                     /gene="phnC"
                     /locus_tag="OO315_002349"
     CDS             26321..27109
                     /gene="phnC"
                     /locus_tag="OO315_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5891236.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27134..>28033
                     /gene="phnD"
                     /locus_tag="OO315_002350"
     CDS             27134..>28033
                     /gene="phnD"
                     /locus_tag="OO315_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5891237.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKG"
BASE COUNT         7210 a         7313 c         6823 g         6687 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaaccct tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaacaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac tggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgatg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgtaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctag atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggccccacc
    13861 ccacgctttt ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta
    13921 taaaaatttg taatattagt aaaactcgtt atttttatgc atgtttatat tcatcatgca
    13981 attatataac catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac
    14041 tcaggagtac atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa
    14101 aagatgtctg gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg
    14161 cgttattaaa ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg
    14221 ctcatattag tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc
    14281 aaataccagt atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag
    14341 acgtctttcg cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg
    14401 aacgaacacg cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc
    14461 tttcattaat gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg
    14521 aaagcgatat tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc
    14581 caagtctgtt aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca
    14641 cctgccgcat gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg
    14701 tatcacagtc ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct
    14761 acggtatgac gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt
    14821 taaccttaac gaagagctat attaataacg gcatcagcga taacccggtc gataataatt
    14881 caactatcga atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc
    14941 aggagtttaa ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc
    15001 ctgatggtgt cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc
    15061 tccactggcg ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc
    15121 tcgatggtat acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac
    15181 gcccgccgct gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc
    15241 tgctggatcg gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc
    15301 ccgattctga aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc
    15361 gtcctgctga acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg
    15421 ctggcgctga tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa
    15481 acctatatgg cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc
    15541 cttaacgtta cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg
    15601 gtgaaaaacc cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc
    15661 gtttgctatg tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc
    15721 gtttctgcct cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc
    15781 attgtttcct tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg
    15841 gcgggtcaaa cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt
    15901 gtaaataaag cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc
    15961 ttccagctca gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc
    16021 tcggtcatct ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc
    16081 cacggtcact ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc
    16141 tactgcattt gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc
    16201 ctgatggtca tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat
    16261 cccttagatg caccaataag caaagattaa ttccccttaa tccagcaaac ataaaagcca
    16321 accttaagaa cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga
    16381 tggaaacacc gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg
    16441 gctattgttg gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt
    16501 gttgcaggcg ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt
    16561 tgtcgctttc agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg
    16621 accactggcc tgcctgttta ttctgattgg cgcggctgca caatatttca taatgactta
    16681 cggcatcgtc atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag
    16741 ttatgcgctg atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctga
    16801 gctgattgcc tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt
    16861 ccgtggagcc aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta
    16921 taaagactac gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc
    16981 taacagcatt gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct
    17041 ggtgcgaatt ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa
    17101 tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta
    17161 cccgcgtgaa actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc
    17221 atcttgcggc acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga
    17281 gcactacaaa gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc
    17341 gggcatcaac gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt
    17401 gcctcaccag aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta
    17461 tgacgaagtg ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt
    17521 gattgtctta cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca
    17581 gttcaggaaa tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca
    17641 actggtgaac acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat
    17701 taatctgctt aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca
    17761 cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc
    17821 ggatagccaa aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta
    17881 tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa
    17941 tttattctcc acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga
    18001 tgatattctg caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat
    18061 acgctgttat tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc
    18121 gtgacaaccg cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta
    18181 ctggatttag ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa
    18241 aaatacaccc tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc
    18301 gggctggatg tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac
    18361 gcccgtatcc gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt
    18421 ggcaatctga cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt
    18481 ctgacgccca aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta
    18541 catcgggaaa ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc
    18601 ctggaagtgc atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg
    18661 cgcggctttg gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt
    18721 ctgcgccgac ctatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg
    18781 gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg
    18841 tttgagcagg cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc
    18901 gaggcggtcg ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc
    18961 tgctatcagg cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa
    19021 gcgcgcaccg ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa
    19081 gcggtggttt cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa
    19141 aggttgttta ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg
    19201 catctggaac tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt
    19261 gatcagatga tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt
    19321 tcttccggta attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac
    19381 gaactcagta ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc
    19441 gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa
    19501 aacgcccatc gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc
    19561 ggagcggtca tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag
    19621 ttgagtaaag cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt
    19681 attgtcagcc gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag
    19741 acttccggca cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata
    19801 taaagaaagc tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg
    19861 tttttcattg cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg
    19921 aaccttaaga gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct
    19981 tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca
    20041 atctcgtggt caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt
    20101 tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc
    20161 gtctctttca tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag
    20221 gcaggcatca tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga
    20281 ccggcaacca gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc
    20341 gggaacatcg ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc
    20401 agcatcagta acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc
    20461 gggatcgcca acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa
    20521 cggtcactca gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata
    20581 ataatcagca ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata
    20641 taggtcagca acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac
    20701 aggctgcgcc agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa
    20761 ccttcgcggt cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc
    20821 agcgcatggc gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata
    20881 cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg
    20941 cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca
    21001 cgtttacggt caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccaccg
    21061 accgagaaac cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc
    21121 gtgtcgtagg acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg
    21181 atagcgagga tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca
    21241 agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg
    21301 gggtcagccc ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca
    21361 aaatcaaacc attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta
    21421 ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc
    21481 atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc
    21541 cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct
    21601 tacaccctcc ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa
    21661 tgaccttttt accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt
    21721 aggactcatt gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca
    21781 ttgcccccac caaacgctgc gctgaacaga gtcgtggcat ccccagcgcg acagtttgcc
    21841 agcgttgggt cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc
    21901 aacttaaaaa atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat
    21961 ggtaatagtg acgcgtagtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt
    22021 tctcgcgcaa atcacccagc aacaacacca ccgcttgcgg gcttcccaga cggcaggcat
    22081 cgccaaatgc accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc
    22141 aggcgttata cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct
    22201 gcaccagtag gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc
    22261 actcatacaa ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat
    22321 ccattttttg cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat
    22381 ccagcatcga cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca
    22441 tgccgccgtt cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc atcagtcgtt
    22501 cacgaaactg ctgctgatgg gcttgaggat ggcgggcatc atcgccctgc gccagatcca
    22561 ctaaaaagcg cggatgaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg
    22621 tcaatatcgt tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gagcgtcggt
    22681 gtaaatccag ctgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac
    22741 cgattcattt tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attctgtcaa
    22801 acatgaagcg aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg
    22861 ctctgccaga actttagcca tttgttggca atagagatcg acttctgcgc ttaatgctcg
    22921 ttgcaacaca ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc
    22981 gggctgattc aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc
    23041 gtccgttgct tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg
    23101 aactacgccg tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc
    23161 cattttgact tgttgctcaa tggggcgaat aacatcggtt aacacccggc aaagggtgga
    23221 ttccatctcg gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa
    23281 cagtgtctca cagcgggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc
    23341 ggctaactgc gcattcaccg catcttgctg ttgacgcaga aagtggttgg cggaggtcag
    23401 ggccagctcg atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag
    23461 ctgcaaactt tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa
    23521 cccgtgcgca cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg
    23581 cgcagcagat cgcagagcat acaatgaggc gttagcgtaa gcggcatgaa gcaacgcctg
    23641 aattggctgg gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc
    23701 gaggtccgcc agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc
    23761 cacccagcgt tgttgctctg gcgctggtaa cttaccgttg ttggctaact catagcgcgc
    23821 ccgattcgcc aggtagcccc acatcgacga caccgggaat atctgctgtg gcgtaataca
    23881 gcctttcatc agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg
    23941 atcctgttga tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa
    24001 aatcgcctca cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc
    24061 cagtaccgcc gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc
    24121 ggcttcattt ggcccggggg tatccagtaa cgtcaactga ccgggataac tttccagccc
    24181 cgccagatgg acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga
    24241 aaaaggaaaa tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag
    24301 acaatgaaaa ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat
    24361 ccgctgcata agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat
    24421 atcgcaatca cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg
    24481 tgaaaaatgc agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc
    24541 agtcattggg cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt
    24601 tgatttccct gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt
    24661 acacaactca ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa
    24721 agccagttgt ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa
    24781 ctgttggcga gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg
    24841 tgtgtacata gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt
    24901 ttttgagttc tgttttcggc ttttataatt actgcaagaa ataattttat atttagtgtg
    24961 ttgttttttt atcagaataa ataacgtctt ctgatgcgtt taaaacgtca gaaagataaa
    25021 aatatcatgt gaataaaaaa aagaacaagt agagcattaa cattatctta aataataaat
    25081 agaggcaaaa agattatttt ctttttgcgt ttcctttcaa atgaaaacga tcgtcgtcta
    25141 aaatcagcag tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc
    25201 aatctgccgt tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta
    25261 ccacactgcc caaaatgcaa ctccgaatac acttacgaag ataacggcat gtacatctgc
    25321 ccggaatgtg cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc
    25381 gttaaagatg ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat
    25441 ctgaaggtga aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc
    25501 ctggttgaag gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg
    25561 aaatctgagt ttgtgaaaaa gaactgattg tattgtgatc ggtaagccgg ataaggcgct
    25621 cgcgccgcat ccggcaacga tgccgactgc ctgatgcgac gcttgcgcgt cttatcaggc
    25681 agcctctcat ctgtataaat ttcgaactac acttaactgg cttctcttaa ctgaggtcac
    25741 catcatgccg ttaagtccct acctctcttt tgccggtaac tgttccgacg cgattgccta
    25801 ttatcaacgt acgttgggcg cggaactgct ctataaaatc agcttcggcg aaatgccgaa
    25861 atcagcgcag gacagcgccg agaactgccc ttccggaatg caatttcccg ataccgccat
    25921 cgctcatgcc aacgtgcgca ttgccggaag cgacatcatg atgagcgatg ctataccgtc
    25981 aggaaaagcc agctactccg gctttacgct ggtgctcgat tcgcaacagg tcgaagaagg
    26041 aaaacgctgg tttgataacc ttgccgctaa cggaaaaatc gaaatggcct ggcaggaaac
    26101 tttctgggcg catggctttg gcaaagtcac cgataaattt ggcgtaccgt ggatgattaa
    26161 tgtcgtcaaa caacaaccaa cgcaataacc cgccgggagg cccgccctcc cgcactgtca
    26221 tcgaattccc gttaactctt catctgttag tcacttttaa ttaaccaaat cgtcacaata
    26281 atccgccacg atggagccac ttttttaggg aggctgcatc atgcaaacga ttatccgtgt
    26341 cgagaagctc gccaaaacct tcaatcagca tcaagcgctg catgcggttg atctgaacat
    26401 tcatcacagt gaaatggtgg ctctgcttgg gccgtcgggt tccggcaaat ccaccctttt
    26461 acgtcattta agcggtttga ttaccggcga taaatccgcc ggtagccata tcgagctact
    26521 gggccgcact gtccagcgcg aaggccgcct ggcccgcgat atccgcaaaa gccgcgccaa
    26581 caccggctac atctttcaac aattcaacct ggtgaaccgc ctgagcgtac tggagaacgt
    26641 gctgattggc gcgctcggca gcacgccgtt ctggcgcacc tgttttagct ggttcaccgg
    26701 cgagcagaaa cagcgcgcgt tacaggcgct gacccgcgtt ggcatggtgc attttgccca
    26761 tcagcgcgtt tccaccctct ccggcggcca gcagcaacgt gtggcgattg cccgtgcgct
    26821 gatgcagcag gcgaaagtga ttctggccga tgaacccatc gcctcgctgg acccggaatc
    26881 agcgcgcatc gtgatggaca ccctgcgcga catcaaccag aacgacggca tcaccgtggt
    26941 cgtcacgctg catcaggtgg attacgccct gcgctactgc gaacgcatcg tcgccctgcg
    27001 tcaggggcac gtcttctacg acggcagcag ccaactgttt gataacgaac gttttgacca
    27061 tctctaccgc agcattaacc gcatcgaaga gaacgcgaaa gctgcctgat atccccatca
    27121 ttgaggaaaa cgaatgaacg ctaagataat tgcctcgctg gccttcacca gcatgttcag
    27181 cctcagcacc ctgttaaccc cggcacacgc cgaagagcag gaaaaggcgc tgaatttcgg
    27241 cattatttca acggaatcac agcaaaacct gaaaccgcaa tggacgccgt ttttacagga
    27301 tatggagaag aaactgggcg tgaaggtcaa cgccttcttt gccccggact acgcgggcat
    27361 tatccagggg atgcgcttca ataaagtgga tattgcctgg tacggcaacc tgtcggcgat
    27421 ggaagcggtg gatcgcgcca atggccaggt cttcgcccag acggtcgcgg cggatggatc
    27481 gccgggttac tggagcgtgt tgatcgtcaa caaagacagc ccgatcaaca acctgaacga
    27541 tctgctggcg aagcggaaag atctcacctt cggcaatggc gatcctaact ccacctctgg
    27601 cttcctcgtc cccggttact acgtcttcgc taaaaacaat atctccgcca gcgacttcaa
    27661 gcgcaccgtc aacgccgggc atgaaaccaa cgcgctggcc gtcgccaaca agcaagtgga
    27721 tgttgccacc aacaacaccg aaaacctcga caagctgaaa acctccgcgc cggagaagct
    27781 taaagagctg aaggtgatct ggaaatcgcc gctgatccta ggcgatccga tcgtctggcg
    27841 caagaatctg tccgaaacta ccaaagacaa gatctacgac ttctttatga actacggaaa
    27901 aacgccggaa gagaaagcgg tgctggaacg cctgggctgg gcgccgttcc gcgcttccag
    27961 cgacctgcaa ctggtgccga ttcgccagct cgcactgttt aaagagatac aaagcgtgaa
    28021 aggcaataaa ggg
//
LOCUS       DAJTRD010000051        27535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTRD010000051 DAJTRD010000000
VERSION     DAJTRD010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.051"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            319..1617
                     /gene="kgtP"
                     /locus_tag="OO315_002351"
     CDS             319..1617
                     /gene="kgtP"
                     /locus_tag="OO315_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5891238.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1614..1937)
                     /locus_tag="OO315_002352"
     CDS             complement(1614..1937)
                     /locus_tag="OO315_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5891239.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO315_002353"
     CDS             complement(1983..3338)
                     /gene="pssA"
                     /locus_tag="OO315_002353"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5891240.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO315_002354"
     CDS             complement(3452..6112)
                     /gene="pat"
                     /locus_tag="OO315_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5891241.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO315_002355"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6144..6842)
                     /gene="tapT"
                     /locus_tag="OO315_002355"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5891242.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO315_002356"
     CDS             complement(6911..7330)
                     /gene="trxC"
                     /locus_tag="OO315_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5891243.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7537..8574
                     /locus_tag="OO315_002357"
     CDS             7537..8574
                     /locus_tag="OO315_002357"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5891244.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO315_002358"
     CDS             complement(8622..9311)
                     /gene="ung"
                     /locus_tag="OO315_002358"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5891245.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9616..9999
                     /gene="grcA"
                     /locus_tag="OO315_002359"
     CDS             9616..9999
                     /gene="grcA"
                     /locus_tag="OO315_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5891246.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO315_002360"
     CDS             complement(10055..10642)
                     /gene="eamB"
                     /locus_tag="OO315_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5891247.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10745..11626
                     /locus_tag="OO315_002361"
     CDS             10745..11626
                     /locus_tag="OO315_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5891248.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO315_002362"
     CDS             complement(11661..12995)
                     /gene="srmB"
                     /locus_tag="OO315_002362"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5891249.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13127..13864
                     /gene="trmN"
                     /locus_tag="OO315_002363"
     CDS             13127..13864
                     /gene="trmN"
                     /locus_tag="OO315_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5891250.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO315_002364"
                     /pseudo
     CDS             complement(13849..15471)
                     /gene="nadB"
                     /locus_tag="OO315_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15727..15882
                     /gene="rseD"
                     /locus_tag="OO315_002365"
     CDS             15727..15882
                     /gene="rseD"
                     /locus_tag="OO315_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5891251.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15879..16454
                     /gene="rpoE"
                     /locus_tag="OO315_002366"
     CDS             15879..16454
                     /gene="rpoE"
                     /locus_tag="OO315_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5891252.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16487..17137
                     /gene="rseA"
                     /locus_tag="OO315_002367"
     CDS             16487..17137
                     /gene="rseA"
                     /locus_tag="OO315_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5891253.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17137..18093
                     /gene="rseB"
                     /locus_tag="OO315_002368"
     CDS             17137..18093
                     /gene="rseB"
                     /locus_tag="OO315_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5891254.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18090..18569
                     /gene="rseC"
                     /locus_tag="OO315_002369"
     CDS             18090..18569
                     /gene="rseC"
                     /locus_tag="OO315_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5891255.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18767..20566
                     /gene="lepA"
                     /locus_tag="OO315_002370"
     CDS             18767..20566
                     /gene="lepA"
                     /locus_tag="OO315_002370"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5891256.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20582..21556
                     /gene="lepB"
                     /locus_tag="OO315_002371"
     CDS             20582..21556
                     /gene="lepB"
                     /locus_tag="OO315_002371"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5891257.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21828..22508
                     /gene="rnc"
                     /locus_tag="OO315_002372"
     CDS             21828..22508
                     /gene="rnc"
                     /locus_tag="OO315_002372"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5891258.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22505..23410
                     /gene="era"
                     /locus_tag="OO315_002373"
     CDS             22505..23410
                     /gene="era"
                     /locus_tag="OO315_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5891259.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23422..24150
                     /gene="recO"
                     /locus_tag="OO315_002374"
     CDS             23422..24150
                     /gene="recO"
                     /locus_tag="OO315_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5891260.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO315_002375"
     CDS             24162..24893
                     /gene="pdxJ"
                     /locus_tag="OO315_002375"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5891261.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24893..25273
                     /locus_tag="OO315_002376"
     CDS             24893..25273
                     /locus_tag="OO315_002376"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5891262.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25693..25773
                     /gene="shoB"
                     /locus_tag="OO315_002377"
     CDS             25693..25773
                     /gene="shoB"
                     /locus_tag="OO315_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5891263.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO315_002378"
     CDS             complement(25967..26227)
                     /gene="yfhL"
                     /locus_tag="OO315_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5891264.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26442..27535
                     /locus_tag="OO315_002379"
                     /pseudo
     CDS             26442..27535
                     /locus_tag="OO315_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         6790 a         6852 c         7222 g         6671 t
ORIGIN      
        1 taagcgaaag gccatccgtc aggatggcct ttttgcattg gcgcagaaaa aaatgcctga
       61 tgcgacgctg cgcgtcttat actcccacat atgacagatt cagcaacgga tgcggtttcc
      121 ccaacttgcc tacttccata cgtgtcctcc ttaccagaaa tttatcctta agctcctcaa
      181 taaccatttt cctgctaact aaattcatgg tttaggttgc ataatgatac gcaacaaatg
      241 tataacattt tctttaccaa aagaaataaa caaaagcgac caacaaaagc atcggattac
      301 ggcaggagac ataatggcat ggctgaaagt actgtaacgg cagacagcaa actgacaagt
      361 agtgatactc gtcgccgcat ttgggcgatt gtgggggcct cttcaggtaa tctggtcgag
      421 tggttcgatt tctatgtcta ctcgttctgt tcactctact tcgcccacat tttcttccct
      481 tccgggaaca cgacgactca actactacaa acagcaggtg tttttgctgc gggattcctg
      541 atgcgcccaa taggcggttg gctatttggc cgcatagccg ataaacatgg tcgcaaaaaa
      601 tcgatgctgt tatcggtgtg tatgatgtgt ttcggatcgc tggttatcgc ctgcctccca
      661 ggttatgaaa ctataggtac gtgggctccg gcattattgc ttctcgctcg tttatttcag
      721 ggattatccg ttggcggaga atatggcacc agcgccacct atatgagtga agttgccgtt
      781 gaagggcgca aaggttttta cgcatcattt cagtatgtga cgttgatcgg cggacaactg
      841 ctagccctac tggttgtcgt ggttttacaa cacaccatgg aagacgctgc actcagagag
      901 tggggatggc gtattccttt cgcgttagga gctgtgttag ctgttgtggc gttgtggtta
      961 cgtcgtcaat tagatgaaac ttcgcaacaa gaaacgcgcg ctttaaaaga agctggatct
     1021 ctgaaaggat tatggcgcaa tcgccgtgca ttcatcatgg ttctcggttt taccgctgcg
     1081 ggctcccttt gtttctatac cttcaccact tatatgcaga agtatctggt aaatactgcg
     1141 ggaatgcatg ccaacgtggc gagtggcatt atgactgccg cattgtttgt attcatgctt
     1201 attcaaccac tcattggcgc gctgtcggat aagattggtc gccgtacctc aatgttatgt
     1261 ttcggttcgc tggcagccat ttttaccgtt cctattctct cagcattgca aaacgtttcc
     1321 tcgccttatg ccgcttttgg tctggtgatg tgtgccctgc tgatagtgag tttttataca
     1381 tcaatcagtg gaatactgaa ggctgagatg ttcccagcac aggttcgcgc attaggcgtt
     1441 ggtctgtcat atgcggtcgc taatgctata tttggtggtt cggcggagta cgtagcgttg
     1501 tcgctgaaat caataggaat ggaaacagcc ttcttctggt atgtgacctt gatggccgtg
     1561 gtggcgtttc tggtttcttt gatgctacat cgcaaaggga aggggatgcg tctttagtga
     1621 cgggtcagtt gccagacggt atagccggtg cttgcaccgg cgacatccca ggccaaatcc
     1681 ttccagctcc agccgctccc ttcggggcgg ctatcccaaa gctcttttga cgcccccaaa
     1741 ctgacagaga acatcaatcc aaacatggca ctgcgatccc ggctcatccc ctgatgctgt
     1801 gaatattcat ttccggcggc ggaaagtatc gccgaggcga taaagtgttg agctttatcc
     1861 tgcccactcc agctatcgtt atccatatgg ctgcattcag aaagtaacaa tagcgaacag
     1921 acaaaaagaa tacgcaaaac gtggcctcca aaaaacaaac cccgtacagg acggggttgt
     1981 gattacagga tgcggctaat taatcggtcg atgcggatac ggcgcaaacg gcggatgagt
     2041 ttacgaacct tcaccggata atcggcaata ctttgcagat cgcgatagtg cttaacgatg
     2101 gtggtacgct cgcggatcag ctccagttct ttctctcgct gcggcgcaag ctccagttgt
     2161 ggatcgtgga tcaaaatggc gttttccaga tccagacgcc aggcgcgcgg gttcaggtta
     2221 ttaccggtga tcaacatcca cttatcatca acccacatcc ctttcaggtg ataggtgttg
     2281 tcgtcatctt tccataaccg aaccactagc tggtcagtat tgacgtaata ctgcaaacgg
     2341 ctcaggaaac gacgcagatt gatctcatag agataaggca atgcgccaat tatcttgaaa
     2401 ggttcatctt ccggaatgta gaagtcgttc gcggttttat caccaacaat aatttcgacc
     2461 tttttccctt cgcgcagcaa ctggataata ttgcgcacaa ggattgctgg caggttgaag
     2521 tatggcgtac agatggttag tttctgctcg gcacaaggca taagatggaa aatggtcttg
     2581 ttcaacagac tcgatttccc cagccccact agcggcgtta cggaaagctg atcgttgtcg
     2641 gcatcgccct ggaaatgata agcggcatca cgcagctcct ggcggaacag acgaatatcg
     2701 ttcttgattt ccgggctttt tggccgatta acatcatcca gacgattaac gccgcggcca
     2761 ttcataatat tctgtgtaac ccattcaaac ataatgtctg acatcttacg gttacggatc
     2821 agatgataac ggtcgtagcg atatttatcg tgttgatgca ggtaaacatc gttcaggctg
     2881 gcaccgctat aaagtacgct atcgtcgatg ataaagcctt taaagtgcag aacaccaaga
     2941 gcttcacggg tattgattgg aacgccataa accggaacat ctacgcccgg attttcctgc
     3001 gccatgcggc agtaccagtc agcgttagtg ttagatgccg cagcgccaat gcgtccacgt
     3061 tgtgcacgat gccagtcgac cagcacccgc acatccagtt ccggacgctg ccttttagcc
     3121 tcatacaacg cgttcagaat gcctttgcca ccgtcatcct gttcgagata cagggcgaca
     3181 atgcaaatgc gctgcttcgc gctggctatt ttttccagca gcgtctcccg gaagtcggcg
     3241 ggagcgtaaa agaaatcgac atcatcaact gattgagaaa tcttgggtag ttgggcaagg
     3301 tgttgttgat gtttattacg cttaaatttt gacaacatca cagtgcattt cttctctgtt
     3361 cattgaaggg tcctctgtgc aatgcagacg acataagcgg gcaataataa caccagtccc
     3421 gattaagtgg tcaacatttc cagtacctta ctcatgattc ctcgcgccgg gcaagattta
     3481 gcgtaagccc aacgatcccc tcttcgagct ggatatcaac gttaaacccg agcttgcggg
     3541 ctagcgccac catgccacga ttgtttggca tcgtaatacc attcagacgt tgtagtccgt
     3601 gatttcgcgt ataggtaatc aacttttcca ttaagcgtcg acctaagcct aaccctttga
     3661 gatccgagcg aaccagtaca gcaaattcgg catcgatgtt atcaggatcg gaaatcgcac
     3721 gcgtgacgcc gaggatctct tccgtttgat caatacgtcg taccgctaca aacgccattt
     3781 cccgatcgta gtcgatctgt gtcatgttgg ctaaatcttc atgggtaaat tcgttgatct
     3841 cgctaaagta gcggtaataa agatcttctt tggtgactcg cgaaatgaat tgctgaagtt
     3901 gtggctcatc ttctggcaaa atcgggcgga acaagcagcg ttcaccgttt ttcaattcta
     3961 cccattcttc cagctgatgc ggataagggc gcactgccag ccgactctcg ttatcgcctt
     4021 caaacggcga gatatccagc gtgacatcca gcgcggtaaa ttcactgcca gaagccagca
     4081 aaggatgaat atccagacgc tgaatttccg ggcaatcgac aatcaagttg gaaacctgca
     4141 ccagaagctg gctcaagcct gcaacatcca atgggcgtag cgcactgcgc gcacgaatct
     4201 ttttactttt gatcccctga ataaccagat agcgggccag gttcatgttc agcggcggca
     4261 gtgcgacgac ggcttgatct tcaggacgcc actccacacc gccttcaccc agcatgatca
     4321 acggcccgaa aaccggatcg tgctcaacca caacccgcaa ctcctgagcg ccagcacggt
     4381 tagccatact ttgcaccaac aggccgtgga cccgcgcctg tggccaggcc atttttacgc
     4441 gatcgaaaat agcgttcgcc gcttgctgga cttcattggc tgtacgcagg taaagcatga
     4501 cgccctgaac ttccgattta tgtggaatat ccggcgaacg caatttcagc gccaccggat
     4561 aaccaatctg ttcggcaata tgcaccgctt cggtgctatc gctggcaatc caggttggga
     4621 gcgtgttcat gccatacgct tgcaggatgg gctgaacttc atgggtatcg agcgacgtag
     4681 ccccttcggc aatcgcctgt tgcaacagaa gatgcgcttc tgcggtattg gaagtcagat
     4741 tgctgggcaa cgccggcgtt tcgcgtagtt gcttctgatt acgccggtac tccaccatat
     4801 gcataaaagc agtgatggtt ccttccgggg tacggtaggt cggcagcccg gcttcgctga
     4861 ataaacgtcg tgcctcttgc gaggagtgct cgccgcacca gttcgtcagc aaagagacat
     4921 atttgctgcg gggatgatgc tttaccgctt caattaatac ttgcgcgctt tctgttgcgg
     4981 gagcagcggc gctgggcgaa tgaataacca tcagtgcatc aaaatcctgg ctgtggagca
     5041 gaatgtccag cgttttgaca taatgctcgc tgctggcgtc atcgcgcaga tcgagcgggt
     5101 tagagactgc cacatgttct ggcaatgcat cgcgcaattt ctggcaggtt tcttcgctta
     5161 gcgttgccag cttgccattg cgtgaccata aggcatccag cgccagcgcg gcaggcgcag
     5221 caccgttgct gataatcatc aaccggtcgc cacgcagcgg gcgcatatgg ctaagggttt
     5281 ccaccgccga aaacagttcg tgggtatcct gtacccgcaa caaaccggca cgctgaatag
     5341 ccgcatccca tgccgggtcc attcctgccg tggtgttgag cagtcgctgt gccgccgggc
     5401 tacgtccgct tttaatcacc agaatcggtt tattacgcga ggcactacgg gccgccgaaa
     5461 caaagcgtcg cgcgtcgctt aactgttcga gatagagcag gatggcgctg gttttactgt
     5521 cgcgcgccag atagtcgagc aattcatcaa cgtcgatatc taggctgtcg ccgagcgcaa
     5581 taaagtagga aaagcccatc tcacgctgtt gcgcccagtc gaggatggtg ttggagacgg
     5641 cagccgattg cgaaataaac gccagcttgc cgcgtttaat cggcacaggc gaaaagctgg
     5701 cattcagacc ttgccaggga gccagtaaac ccagactgtt tggtccaagc aggcgcatgt
     5761 tatggcgcag ggcgcaggcg cggagatctt cgtgttgcga tgccggggcg gaaagaataa
     5821 tgcaggtttt acagcctttc tcgccgagct cttccagaag agcaagatta cggctggcat
     5881 tggtacataa aaccgcaagg tcgggtgtaa agggcaagct ggcaatatcc ggccaggcca
     5941 acacacccaa caccgctttc caggctggcg tcaccgggag taccggtccg ttaaagcctc
     6001 ccgccagcag gttacgcatc atcaggtaac ctgcgcgatt gggtttcatc gacgcgccaa
     6061 ttaccgctat cgattttggt cgcagtagtg cttccagtcc tcgctgactc ataccggtct
     6121 cccgcagaag tgaccgaatg attttaaacg ctttctaact gttctgctgt gatgctaccc
     6181 agatgttgcg tttttcctgc cagatagcgt gttttaaagc gggtaaaatg ctcgcctaac
     6241 cctgctgccg ccccggtatc gccggccata tctaacagtg cgatggctac ctcggcagta
     6301 caatattggc ctgcagcctg ggcttcacgc aggcgatagg cagaaagccg ggaaagatcg
     6361 acggaaatga cgggaagatt atccagatac ggacttttac gaaacatctt gcgagcttcc
     6421 ggccaggtac catcgagcat gataaacagc ggtggcttac cggcaggtgg tgtgaagatc
     6481 acttcccgtt gctcatcagc atacgaggcg ggaaagacca ccattggctg ataatccggg
     6541 ttttgtacca gatccagcaa atcctgcgag ggttcggtac gcgaccattg aaacgcaacg
     6601 gtatcaggca aaatatcagc aatcagacgc ccggtattac tgggcttcat tggctcggtg
     6661 tcgaacatca gcaaacagaa gcgacttttt gcttgtgctg gggtaattgt cgaacagaga
     6721 cataatttct ctggcaaaag acagcgttgg cagcgacgaa cgcgattacc gcgggcaaga
     6781 aaaggacgtg ttgcgcgcgc aatacgctcg gcgcgtaact ggagaacagc gttttcggtc
     6841 ataagagagc gtcgaaaaaa cgccattgtc gcagaggaga aaacggggca caagatgcgc
     6901 cccggtaaga ttaaagagat tcgttcagcc agctatcgaa cggcgctttc ggtactgcgc
     6961 cattaagcat gtcgacaacc tgaccgtttt tgaaaatcat gatcgtcggt atactacgaa
     7021 ttccaaagcg actgctcaat tcacgttcag cttcggtatt cactttcaca aagcgcactt
     7081 taccgctacg ctcttgcgcg acatcttcaa aaattggtgc gaaattacgg caggggccgc
     7141 accacggtgc ccagaagtcg atcaccacag gtagatcatc cttcagcaat ttgtcgagtg
     7201 tttcaccggt cgcattaatc acctctccgt caaacaagtc gtgaccgcag cgtccgcatt
     7261 ttgccgcatc ttcgatccga tcgtcgggaa tgcgattgat ggcctgacaa tgggtacaaa
     7321 cggtattcat aactaacctc gggatgagta aggagactta tcggcaacga cgccggtatg
     7381 tttctaatat gttacatatt atcggggaca ttgttccaga caacaatctg ttttattcaa
     7441 taggtatgat agtcgcaggc tttgctacga aatcatggcg aaccgcgcgg acttcgggta
     7501 atctgcgcgc ttcgcgcagc gctggtggag aaaagcatga acgatgaaat gaaaggtaaa
     7561 agcggcaagg tcaaagtgat gtatgtccgc agtgatgatg attctgataa acgtacccac
     7621 aacccgcgta ccgggaaagg gggcgggcgt ccaggaaaat ctcgtgctga tggtggccgt
     7681 cgccccgccc gcgatgacaa acagagtcag ccccgtgacc gcaagtggga agattcgccg
     7741 tggcgcacgg tttcccgcgc gccgggtgat gagacgccgg aaaaggccga tcacggtggc
     7801 atcagtggta aaagttttat tgatccggaa gtgttgcgtc gtcagcgtgc ggaagaaacc
     7861 cgcgtctacg gcgaaaacgc ctgtcaggca ctgttccaga gccgtccgga agcgattgtt
     7921 cgcgcctggt ttatccagag tgtaacgccg cgttttaaag aagccttgcg ctggatggca
     7981 gcaaaccgca aagcgtacca tgtggtggat gaagcggaat tgacaaaagc gtcaggtacg
     8041 gaacatcacg gcggcgtttg cttcctgatc aaaaagcgta acggtacaac cgtgcagcag
     8101 tgggtaatcc aggcaggcgc gcaggattgc gttctggcac tggaaaacga atctaacccg
     8161 cataacctgg gtggcatgat gcgcagctgc gcgcacttcg gcgtgaaagg tgtcgtggtg
     8221 caggatgcgg cactgctgga gtcgggggcg gctatccgta ccgcagaagg cggcgcagag
     8281 cacgttcagc cgattactgg cgacaacatt gttaacgtgc tggatgattt ccgtcaggca
     8341 ggttacaccg tagtgaccac ttccagcgag cagggtaaac cgctgttcaa aaccagtctg
     8401 ccagcgaaaa tggtactggt gctgggtcag gaatatgaag ggttaccgga tgccgcacgc
     8461 gatccgaacg atctgcgcgt gaagattgac ggtacgggca acgttgccgg gctgaatatc
     8521 tctgtcgcaa ccggcgtttt gcttggtgaa tggtggcgtc agaataaagc ctgataaatc
     8581 agccgggtgg caactctgcc atccggcatt tccccgcaaa tttactcaca ctctgccggt
     8641 aatactggca tccagtcaat cggcgtctcg ccacgttgtt ccagccactg atttgccagc
     8701 acaaaatggt tgcagccaaa gaatccacga tgcgccgaaa gcggcgacgg atgcggtgct
     8761 ttcagtacat gatggcgttg cttatctata atcgcccctt tcttttgcgc atgtgatccc
     8821 cacaacaaaa acaccacgcc ttcgcggtgc tggttaatca ggctgatgac tttatcagta
     8881 aacgtctccc aaccgaggct ggcgtgggaa tgcgcctgac ctgcgcgaac cgtcaacaca
     8941 gtattgagca gcagaacgcc ctgacgcgcc cagctttcga gatagccatg attagggcgg
     9001 gtgaagcccg gaatagtatt ttccagctct ttatacatat tcaataatga cggaggaatg
     9061 gcaatgccgg gacgaacgga aaatgccaga ccatgcgcct gtcccggtcc gtgataagga
     9121 tcctggccga gaatcaccac tttaacgtca cccaactcgg taaagcggaa cgcgttaaag
     9181 acatcttttt gtggtggata gatagtgacg ccggactgcc gctcgctggc gacggtctga
     9241 agggtattaa gaaaataagg ttgttgcttc tcttcagcca gcacgtcatg ccaggttaat
     9301 tcgttagcca tcttgctctc ctgcgaatct tcaatccgcc tagcttaact gcttctttct
     9361 acagaacaaa atcatgctca ttttttagac ttaaacgagt gcaaaaaaat gtaaattggt
     9421 aaaatttcac acccgcataa gttgatgtaa aacaataaaa tccatttacg accacaatcg
     9481 atgtgtggtt tttgttgatt taaatcaaag aatcaagggt ggttgaggag tatatataca
     9541 ctcaagcaac aatggtttta ccaattggcc gcgacaggct gaacaaatca aataattttg
     9601 ccggggaggc atcacatgat tacaggtatc cagattacta aagccgctaa cgccgatctg
     9661 ctgaactctt tctggctgct ggacagcgaa aaaggcgaag cacgttgcat cgttgcaaaa
     9721 gcaggttatg cagaagatga agtggttgca gtaagcaaac tgggtgacat tgaataccgt
     9781 gaagttccag tagaagtgaa accagaagtt cgcgttgaag gtggtcaaca cctgaacgtt
     9841 aacgttctgc gtcgtgaaac tctggaagat gcagttaagc atccggaaaa atatccgcag
     9901 ctgaccatcc gtgtatccgg ttatgcagtt cgctttaact ctctgactcc ggaacagcag
     9961 cgcgacgtta tcgctcgtac ctttactgaa agcctgtaag gcttaagtag caagttacta
    10021 agcggaagag ggcgacctct tccgcttttt ttcgttaata gaaaatgcgt accgcgcaat
    10081 agaccagcaa cagggcaagc acgatattta actggcgacc atactggcga aacaatcgct
    10141 gaaacagatg ccccgccagc gcccagcaca cattgccaaa cgtcccaatc atcgccagca
    10201 aaacgctgac gccaactacc cagcttagcg cctgtgtttg cggcagaaca aacgtcgaca
    10261 gtgccgtaac accgtacaaa atgattttga cgttcacaaa ctgcaaagca aagctggccc
    10321 aaaagctgat tggttttgcc tgaagtccgt cttcctttgt tgggctggtg gcgattttcc
    10381 acgccagcca gacaatatat gccgcccccg cccaactcaa aaggtgtacc gctgccgggt
    10441 caatcactgc cagtgaaaat gaaatgcccg cacacagtaa catcacaatc aaaaatccca
    10501 gactcatccc tgccagcacg cgggtacttt gacgaaatcc atgcgacgta gcagagctaa
    10561 gggcgagaat attgttcggt cctggcgtca tagcggtaat cagggtgtaa gtccaaaaag
    10621 cacttaaaag gatcggtgtc acttgttatc tcctctgttt tggtagacag tacccgacac
    10681 ggaacattta aaaaaatgaa tgtttttata ttctaaattc gatattttcg ataggtttgg
    10741 ggttatggat ctgcgccgtt ttattacgct taaaaccgtg gtggaagagg gttccttttt
    10801 gcgagcttcg caaaaattgt gctgtacaca atcgacggtg acttttcata ttcagcagct
    10861 tgagcaggag ttttcagtcc agttatttga gaaaattggc cgacggatgt gccttacccg
    10921 cgaaggaaaa aagttgctgc cgcacattta tgaacttacc cgagtgatgg atacgcttcg
    10981 tgaggctgcc aaaaaggagt cagatccgga cggggaacta cgcgttgttt cgggcgaaac
    11041 gttgctctct taccgaatgc cgcaggtgtt gcagcgtttt cggcaacgcg caccaaaagt
    11101 gcgtttatcg ttgcaggcgc tgaactgtta cgtgatccgt gatgcgctac tgaatgatga
    11161 ggccgatgtc ggcgtctttt atcgcgtagg gaatgatgat gccctgaacc gacgagagtt
    11221 gggtgaacaa tcgctggtgc tggtggcttc accacaaatt gcagatgtcg attttactga
    11281 gccgggaaga cataacgcct gtagctttat tatcaacgaa ccgcaatgtg tcttccggca
    11341 gatatttgag agcacgctgc gacagcggcg gatcacggtg gaaaacacca ttgagcttat
    11401 aagtatcgaa agcatcaagc gttgtgtcgc ggcgaatatc ggtgtcagtt acttgcccca
    11461 ctttgcggtg gaaaaagaga ttgagagcgg agaattaatt gaattgcctt ttggcgaaca
    11521 gtcgcagacc attacggcaa tgtgcgcaca ttatgctgga aaagcggtta gcccggcgat
    11581 gcacattttt attcagtgtg ttgaagagtg ttttgtggtt gcgtaaaaaa acaatgccgg
    11641 gcttaaaccc ggcattgaga ttactcttct gtcgtttgcg gtggcacgcc cgttccgctc
    11701 ggtttacggc gcttaccaat atttttggtg tcgcgatggc gttttttcac ccgcggcttc
    11761 tctttctctt tagctttttt cttctcggcg cgttttgcca gtactttctt cgatggcttg
    11821 ccggtctgct tctcgcttgg cgcacgcgtt ttcgggcgta actcatcaat aacgcgagct
    11881 ttaatcggct cttcaatgta acggccaacc ttgcccagca gcaaatggtc atgggcttcc
    11941 accagcgaaa ttgcggtgcc tttacgaccg gcgcgcgcgg tacgtccgat acgatgcaaa
    12001 taagtatcgc cactgcgcgg catatcgaag ttaaagacgt ggctgacgtc aggaatgtcg
    12061 ataccgcgcg cggcaacatc ggtggcaacc agtacgttta cgcggccttc ggtcaggcgt
    12121 ttaattgctt cgttacgctt gccttgtacc atctcacctt cgagatagca gttgttgatg
    12181 cccgcttcgc gcagccagtt tgccagctca tgcacacgtt cacgcttacg cacaaacacg
    12241 attgagcgag tcgcttcagg ctgttttaac aggtgcacca gcaatgcggt tttatgttcc
    12301 aggtcatcgg cgcggtaata ccactgatga atttttttgc gctcacgggt ggacggattg
    12361 gcagaaactt ccaccggatc ttccagcaga cgctcggcaa agtcctgaat cgcatcgcct
    12421 tccagcgttg ccgaaaagag cagggtctgt ttacgccagc gagtttcgcc agcaatatgt
    12481 tcgatatcct gagcgaagcc catatccagc atacggtctg cttcgtcgag gatcagcgtt
    12541 tcaaccgcgc ggcaatcgaa gttctcttct tttatgtatt gcagcagacg tccggtcgtg
    12601 gcgaccacga tgtcctgatt ttcgctgaac acttccgcgt ggttcatata ggctacgccg
    12661 ccggtgatgg tggcgatatc cagatgcgta tgtttcgcca gttcgcgggc atgatcggcc
    12721 acctgcatcg ccagctcgcg agttggggtg aggatcaaaa tacgcggcgg accggatttc
    12781 ttacgtggga aatcgagcag gtgctgcaac gctggcagca gatacgccgc cgttttaccg
    12841 gtgcctgtcg gcgcagaacc gagtacatca cggccatcga gcgcaggcgg aatggcggca
    12901 gcctgaatgg cggtcgggcg agtgaaacct ttatcctgga gggcttccag caggctttcg
    12961 tcgagttcaa gttcggaaaa agtcgttaca gtcatgttct acctctgtgt ggggcgctga
    13021 ttatagacgt tacggctgca atcttcatct gtttgtatgg atatcgcttc tcgggtattg
    13081 ttctcacccg gtgctatcgc cgtttttcct gcaaggtttt attttcatgt cacagtctac
    13141 atccgtgctt cgtcgtaatg gatttacttt taaacagttt tttgttgctc acgatcgctg
    13201 tgcgatgaaa gtgggaacgg atggcatttt gctgggcgca tgggcaccgg tggctggggt
    13261 gaaacgttgc cttgatatcg gcgcgggtag cgggttgctg gcactgatgc tggcgcagcg
    13321 aaccagtgac agcgtgataa ttgatgccgt tgagctggaa agtgaagctg ccgcgcaggc
    13381 gcaggaaaat atcaaccagt ccccgtgggc agagcggatt aacgtccata cggcggatat
    13441 tcagcagtgg atcacacagc agacagtacg cttcgattta atcatcagta acccacctta
    13501 ctatcagcag ggagtagagt gcgcgacacc tcaacgggaa caggctcgct ataccactac
    13561 gcttgatcac ccatcgctgc tgacctgtgc ggcggagtgc attaccgaag agggattttt
    13621 ctgcgtggtg ctgccagagc agattggtaa tggttttact gaactggcat taagcatggg
    13681 ctggcattta cgtttgcgga cggatgtggc ggaaaacgaa gcgcgactgc cgcatcgggt
    13741 gctgctggca ttctccccgc aggcgggaga atgctttagc gatcgcttag tcattcgtgg
    13801 gccagaccag aactattccg aagcgtatac ggcgctgacc caggcttttt atctgtttat
    13861 gtaatgattg ccgggggaaa ggatcgatgg accggaatgc gtgagcagtt ccgggtaatc
    13921 cagcgtaaaa tgcagccccc ggctttcttt gcgcatcatc gcgcagcgaa caatcaactc
    13981 ggcaacctgt accagattac gcagctccag caaattattt gagacgcgga aatgggcgta
    14041 atattcgtct atttcttgtt ggagcatggt tatccgccgc agggcgcgtt ccaggcgctt
    14101 cgttgtgcgc acaatgccaa cgtaatccca cataaacagc cgtagctcgt gccagttatg
    14161 ctgaattact acccgttcgt cagggttctc aacgcggctt tcatcccacg gcggtaacgt
    14221 actgacgccg tgggcataag gcatacgtct ggtgatatct tctgccgccg accagccata
    14281 gaccagacac tccagcaatg aattcgaggc catgcggtta gcgccgtgta agccggtata
    14341 actcacctcg ccaatggcat acaagccctc gacgtccgta cgcccatgat catcaaccat
    14401 tacaccacct caggtatagt gtgcagcagg cacaatcggt accggttctt gtgtgagatc
    14461 aatccccagc ccgagcaatt tttcataaat catcgggaaa tgctggcgaa taaaatcggc
    14521 gggcttatgg ctgatgtcga ggaacataca atctgcgccg aggcgtttca tttcatggtc
    14581 aatggcgcgg gcgacaatat cgcgcggggc cagttcgccg cgctcatcaa aatcgggcat
    14641 aaaacgcgtg ccatccgggc gcttgagata agcgccttcg ccgcgcagtg cttccgttaa
    14701 caggaaattg cgtgcctgtg ggtgatacag tgcggtaggg tggaactgat taaattcgag
    14761 attggcaacc cgacagcccg cgcgccacgc catagcaatg ccatcgccag aagaaatatc
    14821 cggattggtg gtgtactgat acaccttcga cgcaccgccg gttgccagta ccactgcttt
    14881 tgcgtggcag gtttccaccg tttctttatt tcggttccat acccacgcgc ctacaacccg
    14941 tcgcgtgccc ggcaggccaa ttttgtcaga gacaatcaga tcaactgcgt tgctgcgctc
    15001 cagcacacga atattcggat ggttctgcgc cttgctcacc agcgtggatt gtacttctct
    15061 accggtggcg tcggcggcat gaagaatacg acggtgacta tgtccacctt cacgggtcag
    15121 atggtagctt tcttcgccat tcggttgaac gtgggtatca aacaaaaccc cctggtcaat
    15181 taaccatggc acacaggaac gagcattgct ggcgacaaat tcaactgcat ggtgatcgca
    15241 aataccagcc ccggcaatca atgtgtcttc cacatgcgag tcaatgctgt cagtttcatc
    15301 aaacacggcg gcaataccgc cctgggcata aaatgttgaa ccttccgtta ccgggccttt
    15361 acttagaacg atgacctgat gctggtcagc caggcgtagc gccagtgaaa gtccggctgc
    15421 gccgctacca ataatcaaca cgtcacatga atgttcaggg agagtattca ttttctttgt
    15481 ttaatttact aaacattgtt tggtcagcat agcatcatgt tgtgcgaata aacacctgct
    15541 attttaatat ttgttacagt ggctaaacac gttgacgcag ggcggcgaga aaaagagaag
    15601 ttactggctg gtggaggatt aggtggtgaa ataaaaaggc cgttgggtta ctcttcaggc
    15661 agttaaatgg gcatttctac acagataatg cgatgttcag attctgtaga cttataatga
    15721 tagataatga tccgtctaca gcatgacaaa caaaaacaga tgcgttacgg aactttacaa
    15781 aaacgagaca ctctaaccct ttgcttgctc aaattgcagc taatggagtg gcgtttcgat
    15841 agcgcgtgga aatttggttt ggggagactt tacctcggat gagcgagcag ttaacggacc
    15901 aggtcctggt tgaacgggtc cagaagggag atcagaaagc ctttaactta ctggtagtgc
    15961 gctatcagca taaagtggcg agtctggttt cccgctatgt gccgtcgggt gatgttcccg
    16021 atgtggtaca agaagctttt attaaagcct atcgtgcgct ggattcgttc cggggagata
    16081 gcgcttttta tacatggctg tatcggattg ctgtaaatac agcgaaaaat tacctggttg
    16141 ctcaggggcg tcgtccacct tccagtgatg tggatgccat tgaagctgaa aacttcgaaa
    16201 gtggcggcgc gttgaaagaa atttcgaacc ctgagaactt aatgttgtca gaagaactga
    16261 gacagatagt tttccgaact attgagtccc tcccggaaga tttgcgcatg gcaataacct
    16321 tgcgggagct ggatggcctg agctatgaag agatagccgc tatcatggat tgtccggtag
    16381 gtacggtgcg ttcacgtatc ttccgagcga gggaagctat tgataacaaa gttcaaccgc
    16441 ttatcaggcg ttgacgatag cgggatactg gataagggta ttaggcatgc agaaagaaca
    16501 actttccgct ttaatggatg gcgaaacgct ggatagtgag ctgcttaacg aactggctca
    16561 taacccagaa atgcagaaaa cctgggaaag ctatcactta atccgtgatt caatgcgggg
    16621 tgatactccc gaggtgctcc atttcgatat ctcttcacgc gtgatggccg ccattgaaga
    16681 agagccagta cgtcaatcgg cgacattgat cccggaagcc cagcctgcgc cgcatcaatg
    16741 gcagaaaatg ccattctggc agaaagtacg tccgtgggcg gcacagctta cccaaatggg
    16801 cgtagccgca tgcgtatcgc ttgcagttat cgttggcgtc cagcactata atggacaatc
    16861 tgaaacgtcc cagcagcccg aaacgccggt atttaataca ctgccgatga tgggtaaagc
    16921 cagcccggta agcctgggag taccttctga agcgaccgca aacaatggtc aacagcagca
    16981 ggtacaggag cagcgtcgtc gcattaatgc aatgttgcag gattacgaac tgcaacgccg
    17041 actccactct gaacagcttc agtttgagca ggcgcaaacc cagcaagccg ctgtacaggt
    17101 gccaggaatt caaactttag gaacgcaatc gcagtaatga agcaactttg gtttgccatg
    17161 tcattagtga caggtagcct gttattctct gctaacgcct cggccactcc cgcgtccggg
    17221 gcgttattac agcagatgaa cctggccagt cagtcactga attacgagct gtcattcatc
    17281 agcatcaata aacagggtgt tgagtctctg cgttatcgac atgcacgcct cgataaccgt
    17341 cctcttgcac aattgttgca aatggatggc ccgcgccggg aagtggtaca gcgcggcaat
    17401 gaaatcagct attttgaacc gggacttgaa ccgttcacgc ttaatggcga ttacattgtt
    17461 gattctctgc catcgcttat ctataccgat ttcaaacgcc tttctcctta ctacgacttt
    17521 atctccgtcg ggcgcacgcg tattgctgat cgtctttgcg aagtcattcg cgtggttgcc
    17581 cgagatggta cacgctacag ctacatcgtg tggatggaca ccgaatcgaa attaccgatg
    17641 cgggttgatc tccttgatcg cgatggtgaa acgctggaac aatttcgcgt gattgctttt
    17701 aacgtcaatc aggatatcag cagcagtatg cagacgctgg cgaaggcaaa tttgccgccg
    17761 ttgctttctg ttcctgtagg tgaaaaagct aaattcagct ggacgccaac ctggttgcca
    17821 cagggtttta gcgaagtttc cagtagtcga cgtccgctac cgacgatgga caacatgcct
    17881 atcgaatcac gtctctattc cgacggatta ttcagcttct cggtaaacgt taaccgcgct
    17941 acgccatcga gcaccgatca gatgttgcgc accggacgca gaactgtcag tacaagcgta
    18001 cgtgataacg ccgaaatcac cattgtcggt gaactgccgc cgcaaacggc gaaacgcatt
    18061 gccgagaata ttaagttcgg ggcagcgcaa tgatcaaaga gtgggctacc gtcgtctcct
    18121 ggcaaaacgg gcaggcgctg gtcagttgtg atgttaaagc ttcatgcagc agctgcgctt
    18181 cacgcgccgg ttgcggtagc cgcgtgttga ataaacttgg cccgcaaacc acgcatacca
    18241 ttgtcgtacc ctgtgatgag ccgttagtgc cggggcaaaa agtggagttg gggatcgccg
    18301 aaggcagcct gcttagctcc gcattactgg tttatatgtc gccgctggtg ggattattcc
    18361 tcatcgcttc gctatttcag ctactctttg cttctgacgt cgcagcatta tgtggtgcgg
    18421 tcctcggcgg tattggcggg ttcctgattg cgcgcggcta ctcgcgtaag tttgctgccc
    18481 gggcagaatg gcaaccgatc atcttaagcg tggcattgcc gcctggcctt gtgcgatttg
    18541 aaacatcttc ggaagacgcg agccagtgat ttcactggct ttgttgcatt ttgcataaca
    18601 aatgcgagga cgtttccaga taccacaatc catacggtat gaacatttcc tcgcctcaat
    18661 gttgtagtgt agaatgcggc gtttctatta atacagacgt taagctcaga acagcgactt
    18721 ctaaaagcct ggttaaccgg gcattaaggc acaataatca tactttatga agaatatacg
    18781 taacttttcg atcatagctc acattgacca cggtaaatcg acgctgtctg accgtattat
    18841 ccagatctgc ggtggcctgt ctgaccgtga aatggaggcg caggttctcg attccatgga
    18901 tcttgagcgt gagcgtggca ttaccatcaa agcgcaaagc gtgacgctgg actacaaagc
    18961 gtctgacggc gaaacctatc agcttaactt tatcgacacc ccgggccacg tagacttctc
    19021 ctatgaagtt tcccgttcgc tggctgcctg tgaaggtgca ttgctggtgg tcgacgccgg
    19081 gcagggcgta gaagcgcaaa ccctggcaaa ctgctacacc gccatggaaa tggatctcga
    19141 agtggtgccg gtactgaaca agattgacct gccggcagcc gatcctgaac gcgtggcgga
    19201 agaaattgaa gatatcgtcg gcatcgacgc caccgatgca gtgcgctgtt cagcgaaaac
    19261 cggcgttggc gtgcaggacg ttctcgaacg tctggtgcgc gacattccgc cgccggaagg
    19321 cgatccggaa ggcccgttgc aggcactaat tatcgactcc tggttcgaca actacctggg
    19381 cgttgtttca cttatccgta ttaaaaacgg caccctgcgt aagggcgaca aagtgaaagt
    19441 catgagtacc gggcagacct ataacgccga ccgtctgggc atcttcacgc cgaaacaggt
    19501 tgaccgcact gaactgaaat gtggcgaagt aggctggctc gtatgtgcga ttaaagatat
    19561 ccacggcgct ccagtcggcg ataccttaac gctggcgcgt aatccggcag aaaaggcgct
    19621 gcctggcttt aagaaagtca aaccgcaggt atacgccggt ctgttcccgg taagttccga
    19681 cgactatgaa gccttccgtg acgcgctggg taaactcagc ctgaacgatg cctcactgtt
    19741 ctatgagccg gaaagctcca gcgcgctggg ctttggtttc cgctgcggct tccttggcct
    19801 gctgcacatg gagatcatcc aggagcgtct ggaacgtgaa tacgatctgg atctgatcac
    19861 cactgcgccg accgtagtgt atgaagttga aaccacgtca agggaagtta tctacgtcga
    19921 cagcccatcc aagctgcctg cggtaaataa catttacgaa ctgcgcgagc cgattgcaga
    19981 gtgtcacatg ctgctgccgc aggcatatct cggcaacgtt attacgctgt gcgtagaaaa
    20041 acgcggcgtg cagaccaata tggtttacca cggtaatcag gtggcgctga cgtacgagat
    20101 cccgatggcg gaagtggtgc tcgacttctt cgatcgcctg aaatctacct cgcgtggtta
    20161 tgcgtctctg gattacaact tcaaacgctt ccaggcgtcc gacatggtac gtgtagacgt
    20221 attaatcaac ggtgaacgtg ttgatgcgct ggcgttgatc acccaccgtg ataattcgca
    20281 aaaccgcggt cgcgagttgg tggagaagat gaaagatctg atcccacgcc agcagtttga
    20341 tatcgccatt caggcagcga ttggtacgca cattattgcg cgatccaccg tgaaacagct
    20401 gcgtaaaaac gtactggcta aatgttatgg cggcgatatc agccgtaaga aaaagctgct
    20461 gcagaagcag aaagaaggta agaaacgcat gaagcagatc ggtaacgttg agctgccgca
    20521 ggaagcgttc ctcgccattc tgcacgtcgg caaagacaac aaataaccct taggagttgg
    20581 catggcgaat atgtttgccc tgattctggt gattgccaca ctggtgacgg gcattttatg
    20641 gtgcgtggat aaattctttt tcgcacctaa acggcgggaa cgtcaggcag cggcgcaggc
    20701 ggctgccggt gactcactgg ataaagcaac gttgaaaaag gttgcaccga agcctggctg
    20761 gctggaaacc ggagcttctg tttttccggt gctggctatc gtattgattg tacgttcgtt
    20821 tatttatgaa ccgttccaga tcccgtcagg ttcgatgatg ccgactctgt taatcggtga
    20881 ttttattctg gtagagaagt ttgcttatgg cattaaagat cctatctacc agaaaacgct
    20941 gatcgaaacc ggtcatccga aacgcggcga tatcgtggtc tttaaatatc cggaagatcc
    21001 aaagcttgat tacatcaagc gcgcggtggg tttaccgggc gataaagtca cttacgatcc
    21061 ggtctcaaaa gagctgacga ttcaaccggg atgcagttcc ggccaggcgt gtgaaaacgc
    21121 gctgccggtc acctactcaa acgtggaacc gagcgatttc gttcagacct tctcacgccg
    21181 taatggtggg gaagcgacca gcggattctt tgaagtgccg aaaaacgaaa ccaaagaaaa
    21241 tggaattcgt ctttccgagc gtaaagagac actgggtgat gtgacgcacc gaattctgac
    21301 agtgccgatt gcgcaggacc aggtggggat gtattaccag cagccagggc aacaactggc
    21361 aacctggatt gttccgccgg gacaatactt catgatgggc gacaaccgcg acaacagcgc
    21421 ggacagccgt tactggggct ttgtgcctga agcgaatctg gtcggtcggg ccacggctat
    21481 ctggatgagc ttcgataagc aagaaggcga atggccgact ggtgtgcgct taagtcgcat
    21541 tggcggcatc cattaatagc catcttcgtt cacgttgtcg ccgttatggc gacaacgtga
    21601 attatttatg agataaatct cccgtggcta acgacatccc ccgtcgttgt gtatagaata
    21661 ttcccccgaa gtttaaggtt ggcaccttca ggttgccacg gcacacgaaa cagcgttggt
    21721 ccccatatac cgctaaactg aaactgcagc gaagcagtta gcagagccat gtatatcagg
    21781 tctgtttcgt gtgctgaatt gttgacgcat ttatttattg gtatcgcatg aaccccatcg
    21841 taattaatcg gcttcaacgg aagctgggct acacttttaa tcatcaggaa ctgttgcagc
    21901 aggcattaac tcatcgtagt gccagcagca aacataacga gcgtttagaa tttttaggcg
    21961 actctattct gagctacgtt atcgccaatg cgctttatca ccgtttccct cgtgtggatg
    22021 aaggcgatat gagccggatg cgcgccacgc tggtccgtgg caatacgctg gcggaactgg
    22081 cgcgcgaatt tgagttaggc gagtgcttac gtttagggcc aggtgaactt aaaagcggtg
    22141 gatttcgtcg tgagtcaatt ctcgccgaca ccgtcgaagc attaattggt ggcgtattcc
    22201 tcgacagtga tattcaaacc gtcgagaaat taatccttaa ctggtatcaa actcgtctgg
    22261 acgaaattag cccaggcgat aaacaaaaag atccgaaaac gcgcttgcaa gaatatttgc
    22321 agggtcgcca tctgccgctg ccgacttatc tggtagtcca ggtacgtggc gaagcgcacg
    22381 atcaggaatt tactatccac tgccaggtca gcggcctgag tgaaccggtg gttggcacag
    22441 gttcaagccg tcgtaaggct gagcaggctg ccgccgaaca ggcgttgaaa aaactggagc
    22501 tggaatgagc atcgataaaa gttactgcgg atttattgcc atcgtcggac gtccgaacgt
    22561 tggcaaatcc acattgttga acaaactgct ggggcagaaa atctccatca cctcccgcaa
    22621 ggcgcagaca actcgtcacc gcattgtggg gatccatact gaaggcgcgt atcaggcgat
    22681 ctacgtcgat acaccgggcc tgcatatgga agaaaaacgc gccattaacc gcctgatgaa
    22741 caaagcggcg agcagctcta ttggcgatgt tgagctggtg atttttgtcg ttgaaggcac
    22801 ccgctggacg ccggacgacg aaatggtgct caacaaactg cgcgaaggca aagcgccggt
    22861 aatcctcgcg gtgaacaaag tggacaacgt gcaggagaaa gccgatctgc tgccgcacct
    22921 gcagttcctg gcaagccaga tgaacttcct cgatatcgtg ccaatctctg ccgaaaccgg
    22981 gctgaatgtt gacactattg cggcaatcgt gcgtaagcat ctgcctgaag cgactcatca
    23041 cttcccggaa gattacatca ccgatcgctc acagcgtttt atggcgtctg aaatcatccg
    23101 cgaaaaactg atgcgtttcc ttggcgctga actgccgtac tccgtgaccg tggagatcga
    23161 acgtttcgtc tctaacgaac gcggcggttg tgacatcaac ggtttgattc tcgttgagcg
    23221 tgaagggcag aagaagatgg ttattggcaa caaaggggcc aagatcaaaa ccatcgggat
    23281 tgaagcgcgt aaagacatgc aggaaatgtt cgaagcgcct gtccaccttg aactgtgggt
    23341 aaaagtgaaa tcgggttggg ccgacgacga acgcgcactg cgcagtctcg gttatgttga
    23401 cgatctttaa gagtcactcc gatggaaggc tggcagcgcg catttgtcct gcatagtcgc
    23461 ccgtggagcg aaaccagcct gatgctggac gtcttcacag aggaatcggg tcgcgtgcgt
    23521 ctggttgcca aaggcgcacg ctctaaacgc tctactctga aaggtgcatt acagcctttc
    23581 acccctctct tgctacgttt tggcgggcgt ggcgaagtca aaacgctgcg cagtgctgaa
    23641 gctgtctcac tggcgctgcc attaagcggt atcacgcttt acagcggtct gtacatcaac
    23701 gaacttctct cccgcgtact ggaatacgag acgcgcttct ctgaactctt tttcgattac
    23761 ttgcactgca ttcagtctct tgcaggtgac actggtacgc cagaacccgc gctgcgtcgc
    23821 tttgaactgg cactactcgg gcatctgggc tatggcgtca attttaccca ttgcgcgggt
    23881 agcggcgagc cggtagatgg caccatgacg tatcgttatc gcgaagaaaa agggtttatc
    23941 gcaagcgtcg ttatcgacaa taaaacgttc accggaaggc agttaaaagc gttaaacgcg
    24001 cgggaatttc ctgacgcaga cacactacgc gccgcgaaac gctttacccg aatggcgctt
    24061 aagccgtatc ttggcggtaa accattaaag agcagggaac tgttccggca gtttatgcct
    24121 aagcgaacgg tgaaaacaca ttatgaatga tgaggattgt catggctgaa ttactgttag
    24181 gcgtcaacat tgaccatatt gcaacgctgc gtaacgcacg cggtactgct tacccggatc
    24241 cggtgcaggc ggcgtttatt gccgagcagg cgggagcgga cggcattacc gtgcatttac
    24301 gtgaagatcg ccgccacatt actgaccgcg atgtgcgcat cctgcggcag acgctggata
    24361 cccgaatgaa tctggagatg gcagtgaccg aagagatgct ggcgatcgcc gttgagacga
    24421 agccacattt ttgctgcttg gtaccggaaa agcgtcagga agtaacaacc gaaggcggcc
    24481 tggacgttgc agggcagcgt gaaaaaatac gcgatgcctg caaacgtctg gcagatgccg
    24541 ggattcaggt ttctctgttt atcgacgccg atgaagagca gatcaaagct gcggcagagg
    24601 ttggcgcacc gtttatcgag atccacaccg gttgctatgc tgatgccaaa actgacgccg
    24661 aacaggcgca agagctggtg cgtatcgcca aagccgcgac ctttgccgct agtctcggtc
    24721 tgaaagttaa cgccggacac ggtctgacct atcacaacgt gaaagccatt gccgccatcc
    24781 ctgagatgca tgaactgaat atcggtcatg ccattattgg ccgtgcagtg atgaccggac
    24841 tgaaagatgc ggtggcagaa atgaagcgtc tgatgctgga agcgcgtggc taatggcaat
    24901 attaggttta ggcacggata tcgtagagat cgctcgcata gaagcagtga tcgcccgatc
    24961 cggtgagcgc ctggcacgcc gcgtattaag cgataacgaa tgggcaatct ggaaaacgca
    25021 ccaccagccg gtgcgttttc tggcgaagcg ttttgctgtg aaagaagccg cagcaaaagc
    25081 gtttggcacc gggatccgca atggtctggc gtttaatcaa tttgaagtat tcaatgatga
    25141 gctcggcaaa ccacggctac ggctatgggg tgaggcatta aaactggcgg aaaagctcgg
    25201 cgttgtaaat atgcatgtaa cgctggcaga tgaacgccac tatgcttgtg ccacggtaat
    25261 tattgaaagt taaaataata ccgttctctt taaaaagtaa aacccccgaa ctggtaagga
    25321 acgggggttt tactttttaa ctttaatttt gtgcagcatg caccctcaac aacagcgatt
    25381 atggatcagt gtacttatct tttacaagca acttgcaatc tttagcataa aaactcgagc
    25441 ctttacgaag aaagcaatat tgatggaaag attaacgtga ccgccaattc gtaagtacat
    25501 taaaattggc ttcgttattg aagattttgc tgtgctttac accatgccac agaattcccc
    25561 cattgaaacg agtggtgtcg tcaaagctct ggtgtggagt gcagcatgca ccctcaataa
    25621 ctcgcacgtt cagttttggg gagatgtaag ggctaatctg aatggctgca ttccttgttt
    25681 aaggaaaaac gaatgactga ttgccgatac ctgattaaac gggtcatcaa aatcatcatt
    25741 gctgttttac agctgatcct tctgttctta taacacaagg aaacgtactt aaggtgcgtc
    25801 cggtgaacca atcggacgcg cctttaataa ctataaataa gtgtcttggc agatactata
    25861 taaattaact tagtgaatga ttatgctaat gtcatcaatt aaataaatat aatggcgtta
    25921 aggcttccca gtaatataat taatactcta cttccagagt agaatattaa attttatccg
    25981 cgtggtgcat cagcacaaat ttatcccaca actgttcttc tgtctcgaca tgcgccggat
    26041 ctttcacaat agtattgggg atcgggcaca ccttctggca ggttggtgtc tcgtagtgcc
    26101 ctacgcattc ggtacactta tcgctgttaa tttcgtagat atgatctccc attgaaatcg
    26161 cctcattcgg gcattcgggt tcacacatat cacaattaat acagcgttta gtaattagta
    26221 aagacatttc aatggattac cgttaaatca ttttaaaatc agtaagttgt atcgagtttg
    26281 tatgctttac tgtcattaac ttactgtatg ttgatccagt gtatttaacc ttgataaact
    26341 cagtccagca acacaaaacc gcaacacatt gcattttgtc ccgtagaaaa gacttgtatg
    26401 tgtgagcttg ttttctgcgc ctacgcagat aaggattgag aatgccgcgc actgtaacac
    26461 ataatccgga tagccccaat aatgacgatg ttttagccgc atctgaaaaa tgggacgcct
    26521 gtaaaccccc ctataccagc gcacacatga aaatctgtgt tgctgccgcc aaaatcatcc
    26581 tcgctgcttc cggcgtggct cgctgttcca aatacgaaaa agagaactat ctccgtatcg
    26641 atttcagcaa agccggtaag gttacatttt acgccgagtt tccaaaaaag atgggcctca
    26701 aaggtaaaaa gctcggcgag tggccggagc tcgttatcca gctggcgcgc gaaaaagcac
    26761 taggtatggc tgacggtgga ctgcgggcag agtccgtaca tgcagcgctg gaaatgtacc
    26821 gggatgaccc aaagccaaag tagcccggca gaagctgagc ccggacagtt tcacaaccta
    26881 cggggtgcgt atcgaccgga ttaaagtaac gttcggcgag cgtgaggtgt tcagcgacgt
    26941 aacatacaat cggctggtgg aagtgctgga cgagtggatc gccactcgct cgaacaataa
    27001 cgccctggag ttgtttgccg agctccgtcg gttctggaag ttctgcgcac ctactctttg
    27061 caacggccgc aatgttgccg ccagtctgcc agatgattat gtttcctccc gcgtacagaa
    27121 acctaccccc acacggcttt ttaccgatat tgaatcaatc gcccgactct ggctcaatgt
    27181 tgctgcctgc acctctgtac accagaagaa tgctgttcgc ttcatgatca tcactggtgt
    27241 tcgtccgatt aatgtccata acctgcgctg ggactacgtt tacgaggagg ctggtgaaat
    27301 tgtttatccg gaaggggtta tcggcatgcg aggggctatg aaaacacaaa aggctttccg
    27361 cctgccgata acgcctgaga tccggcggat tatcgacgag cagaaagcct ggcgtgattc
    27421 agttcctgag tgcaacaggg attatgtatt tttgcagcca cgtgatccaa tgcagccatt
    27481 ttcaaaacga tcactggata agctggtgaa aacatacagc ccggacgggg ctgta
//
LOCUS       DAJTRD010000052        26634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTRD010000052 DAJTRD010000000
VERSION     DAJTRD010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.052"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            235..342
                     /locus_tag="OO315_002380"
     CDS             235..342
                     /locus_tag="OO315_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5891265.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            718..825
                     /locus_tag="OO315_002381"
     CDS             718..825
                     /locus_tag="OO315_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5891266.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(912..2591)
                     /gene="bcsG"
                     /locus_tag="OO315_002382"
     CDS             complement(912..2591)
                     /gene="bcsG"
                     /locus_tag="OO315_002382"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5891267.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2588..2779)
                     /gene="bcsF"
                     /locus_tag="OO315_002383"
     CDS             complement(2588..2779)
                     /gene="bcsF"
                     /locus_tag="OO315_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5891268.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2776..4347)
                     /gene="bcsE"
                     /locus_tag="OO315_002384"
     CDS             complement(2776..4347)
                     /gene="bcsE"
                     /locus_tag="OO315_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5891269.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            4620..4808
                     /locus_tag="OO315_002385"
     CDS             4620..4808
                     /locus_tag="OO315_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5891270.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4820..5572
                     /gene="bcsQ"
                     /locus_tag="OO315_002386"
     CDS             4820..5572
                     /gene="bcsQ"
                     /locus_tag="OO315_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5891271.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            5569..5928
                     /locus_tag="OO315_002387"
     CDS             5569..5928
                     /locus_tag="OO315_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5891272.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5925..8186
                     /gene="bcsA"
                     /locus_tag="OO315_002388"
                     /pseudo
     CDS             5925..8186
                     /gene="bcsA"
                     /locus_tag="OO315_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            8197..10536
                     /gene="bcsB"
                     /locus_tag="OO315_002389"
     CDS             8197..10536
                     /gene="bcsB"
                     /locus_tag="OO315_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5891273.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            10543..11649
                     /gene="bcsZ"
                     /locus_tag="OO315_002390"
     CDS             10543..11649
                     /gene="bcsZ"
                     /locus_tag="OO315_002390"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5891274.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            11726..12067
                     /locus_tag="OO315_002391"
     CDS             11726..12067
                     /locus_tag="OO315_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5891275.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11982..12233
                     /locus_tag="OO315_002392"
                     /pseudo
     CDS             11982..12233
                     /locus_tag="OO315_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            12315..14303
                     /gene="hmsP"
                     /locus_tag="OO315_002393"
     CDS             12315..14303
                     /gene="hmsP"
                     /locus_tag="OO315_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5891276.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            14490..15776
                     /gene="dctA"
                     /locus_tag="OO315_002394"
     CDS             14490..15776
                     /gene="dctA"
                     /locus_tag="OO315_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5891277.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            15997..17493
                     /locus_tag="OO315_002395"
     CDS             15997..17493
                     /locus_tag="OO315_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5891278.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(17589..18518)
                     /gene="kdgK"
                     /locus_tag="OO315_002396"
     CDS             complement(17589..18518)
                     /gene="kdgK"
                     /locus_tag="OO315_002396"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5891279.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            18750..19517
                     /gene="pdeH"
                     /locus_tag="OO315_002397"
     CDS             18750..19517
                     /gene="pdeH"
                     /locus_tag="OO315_002397"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5891280.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            19587..21647
                     /locus_tag="OO315_002398"
     CDS             19587..21647
                     /locus_tag="OO315_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5891281.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(21829..23151)
                     /locus_tag="OO315_002399"
     CDS             complement(21829..23151)
                     /locus_tag="OO315_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5891282.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(23562..24575)
                     /gene="yhjD"
                     /locus_tag="OO315_002400"
     CDS             complement(23562..24575)
                     /gene="yhjD"
                     /locus_tag="OO315_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5891283.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(24624..25523)
                     /locus_tag="OO315_002401"
     CDS             complement(24624..25523)
                     /locus_tag="OO315_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5891284.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            26043..>26634
                     /locus_tag="OO315_002402"
     CDS             26043..>26634
                     /locus_tag="OO315_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5891285.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLN"
BASE COUNT         6364 a         6679 c         7271 g         6320 t
ORIGIN      
        1 gaagacgtga cggattccgg aacagaaaaa cagtcgtaga atcagcgtca agccggaagg
       61 gttccggtga ggcgcaatgt tgcgggggct ttatccctgg tggcattggt tgctggagag
      121 agaaaacccc cgcacgttgc aggtatgcac ctgacaacat cacgggggcc attcttgaac
      181 ctgaacaact caagaatagc cgcgaaacgt tgtcattaca acacaggcgg ctatatgacg
      241 ctcgcagaac tgggcatggc cttctggcat gatttagcgg ctccggtcat tgctggcatt
      301 cttgccagta tgatcgtgaa ctggctgaac aagcggaagt aacgtgtcat gcgggcgtca
      361 ggctgccgta atggcaattt gcgcccggac caggccgcag gggtgaaact ctgcggcctt
      421 tttcgtaaga aaaatgcaac gattgcacgc ggataatatt ctgcggaatg cgcagcacag
      481 agtgaagacg tgacggattc cggaacagaa aaacagtcgt agaatcagcg gcaagccgga
      541 aaggttccgg tgaggcgcaa tgttgcgggg gctttatccc tggtggcatt ggttgctgga
      601 gagagaaaac ccccgcacgt tgcaggtatg cacctgacaa catcacgggg gctaatcttg
      661 aacccaaaca actcaagaat agccgcgaaa cgttggcatt acaacacagg cggctatatg
      721 acgctcgcag aactgggcat ggccttctgg catgatttag cggctccggt cattgctggc
      781 attcttgcca gtatgatcgt gaactggctg aataagcgga agtaacgtgt catgcgggcg
      841 tcaggctgcc gtaatggcaa tttgcgcccg gatcaggccg cagaggttaa actctgcggc
      901 ctttttcgtg cttactgcgg gtaaggcacc cagtcgccgc cgttcaggcg aacgtacggt
      961 ttatcttggt attgaataac tactgcattt gagttctcgg agaccggtgc tgtttgtggc
     1021 aacccactgg tgagtttttt ccagtcaaca ttgtcttcgg tgaaaatctt gccatcaaga
     1081 acgcgaacca ccagatcgga gatagccagg aagctgctcg gttggtcaat gacaatcggt
     1141 gccccctgat gtggtgcctt catgccgaag aatttcaccc caacggggac gtctgtgata
     1201 gacgggctag ggatatcacg taggccagat acctgcattc tgtcgccctt cagcgcgccg
     1261 ccgtgttccg gcaccacgac caccatcact ttacgacccg atttctccag ttcagtaaag
     1321 aaggcgtcca gttcatcaaa gaatttctgc gcccgcgctt tgtaatccgc tgttttgctg
     1381 acccccggat aatggttgcc gtcatgcagt ggaagcgtgt tgtagaacgt ggcactacgg
     1441 ctgtttttat ctttttcggt aacgtccagc cagcggttaa gcacggcggt atcgtcataa
     1501 accggcgaac catcaaagcc cagcaaaata accggcagat ttgtttgatc catcaattca
     1561 gtctgcatgc cgccattttc gcgaacttct ttcaaaaaac cgccgaactg gccgttatgt
     1621 cccatcatca ggtgctgggt aaagcccagt ttcgaaaggt tatcaaacag atagcagtcg
     1681 ttatttgccg gttgatacag attagtgtgc gaagtctgcc cgcagctggc gcgcagtaaa
     1741 cggatcgccg ccgggccact gtaggaggtg gcggagttaa agttcttgaa atcaatatcg
     1801 aaatgcgacc acagtggatg cgacatcaac ccggcggctt ctatatccga ccaggaaagc
     1861 gaacagatgt taatcaccag tagttcaaat ggctgagcat cagcgggcag cgaagacggg
     1921 aaggtcgatt tacgtttcgc ctccgcgtta tagaaattat tcagccaggc gttaaggttc
     1981 gccgttgttg gcggtgcagt ttgtgcgggc atatcaccca ctaccggtgc gccacccgtc
     2041 gccgcaacgg ttgccgctgc gttaccaccc gtcgttgtta cagtggtcgt cggttgtccg
     2101 gctggccaca aggagaaact tggtcccgcc agggtaagta cgttcagcca tagcagtatg
     2161 gcaaccacaa aaacggtaat gcgaatccat tgtgacagga ataaccaggc cactaataaa
     2221 acaaaaatgg ccccaatcat ctgccagtta ataaagcgtg tgacaaggtc gattaaataa
     2281 tcggtactga accccgccac ctgcgaaccc tggctcatta tgctttccgg gccaggcaac
     2341 cagatgtcat gccagaacaa agcaaagccg atcggcaggg caatccagtg gcgcaagcga
     2401 tgcaggctgt aacgcggaat gggcatcagc agaaacgcgg caaacaccaa attgaggagc
     2461 gggtggaagt taagatatcc cgcccacaac aggccaaact taaccagaaa atagaagttc
     2521 cagccggaaa ggccgcgcca gtattgccag agggaagaag gcatggcggt attttgcgta
     2581 aattgagtca ttttttggtt gccctggctt tttccgtgcg gcgtaacgtc ccggccggtt
     2641 taacataacg aggtttagca aagaacaaac gtaaggtgtc gcgaatgcgt cgcaaagagt
     2701 gccgcgccag atagcccagc gggaaaaata tcagtgcgca aacgataata atttcaatga
     2761 tatcgctgat ggtcatcatg atgagcgctc cacagcatca tctaacagtc gcatgggttc
     2821 tggtattcgt cgccagtggc gaccatcgtg ctcggcattg atgaccggtt tagaactttg
     2881 cgttaaaggc agcggcattc cccattgttc tggggcaagc aggcgcatct gcaccagctc
     2941 ggcactgatt tgatcatctt caaaccagac catacggttt gagaaaatgt cgccagtagg
     3001 caatgggaaa atatgattca acgcggtatc cagatcgtta atccgacaga atgagagaaa
     3061 cagcaccagc cgattaccgc caatggtcat gatatcgccg gtacggttag ggcgacatag
     3121 cgtcagggct tgctcaacgc ggatacccgg taccggacgt agggcaacca gaacgccttt
     3181 accgtgggca ggcaagagag ggttattcat catgttgttg acggcattac agaacacatc
     3241 ccacttctgg aaaccacgca gtttgagcgg ctgggtcatt gacagcaagg tagtgatatc
     3301 ttccggtaca tagcgactaa acttctgccc ttgcacgctt tcgatcatcg tcagacaacg
     3361 ggagagtggc gcattccacg gaataaccat atttgcaccg caggccaata acaaacgttc
     3421 atcggtggcg cgcaggctag cggtattttc ccgcacgagg attttcatcg cactaccgcg
     3481 ctggcgacgc agggtatgaa tgctgcgggc cagtggctcg atttgcgcat tttgctgtaa
     3541 agaaaagacc accgtcgccg cctgagcggt acgggcttca ttgaacagga cttcgttatt
     3601 gttgaacagt tgccagtgtt ccgatagcgg cggcgcacct tccagtacag caacattact
     3661 cagaatgcgt ttttcgtcgc tgcgtggttg gatctccgcc tcttcgcttt gaactaatgt
     3721 ccagatacca ttttgttgct gaacgctaag ctgctgacgg gcgctgaccc ctttttcgtt
     3781 gcaccagaag gcaatatcca gcaaatgttg gtcaccctga aaacgcaaac tggcaagacc
     3841 aaaaagtgaa cggtattcct caagcaacaa tgaaaattgt ttatcgttat tatttccggg
     3901 attaattacc aaaagcgaac aatggtttaa cctgctccat ttattcattt tatccaacca
     3961 tgagcgaagc cgctcggcag gaatattttg ccatgcgtta tttgcgcaaa caagaataaa
     4021 aaggtaatta tggggatcaa tagaacactg caaatcacgg gccaaatagt atagaccttt
     4081 ttcatgattt agcattgaaa ataattttat tttttccggc ccttgagaat catctaattg
     4141 aaagattttc gccggatcgc tgtccatgct aatgaccgcg acgtgtgcgg tttcagcctg
     4201 ggatgcaatt gtttgattcg ccagactgat agcatcttca tggcgatcga cgttaaacca
     4261 ccagacgccg cctgctggca tatgtcgcag ctcatcccat aatgatgaga taccgataga
     4321 gaatacaggg tccacaatgt ccctcatgtc accattttgt ctgattatca gtttactagc
     4381 gaaagctaag aaataaacct accattgaaa ttaaaactta tcagatttag catgtgaatc
     4441 aatttagaag gtcacaagac gtgtgatttt agtcttttcg tttttaactt tttctgttgc
     4501 gtaacggaaa gtcaaaaagt gagcaaattc ccgtctcgcc gtaaacaata aaccggcgaa
     4561 atgctcacgg ttagaattgt ctcatgaatg gtacggttat ttcataggga tcaagcaaaa
     4621 tgaataacaa tgaaccagat actctgcctg atcccgcgat aggctatatc ttccagaatg
     4681 atattgtggc gttaaagcag gcattttcac tgcctgatat tgattatgcc gatatttccc
     4741 aacgcgaaca gttggccgcg gcattaaaac gctggccgtt gctggcagag tttgcgcaac
     4801 aaaagtaggg gattggtgaa tggccgtact gggattgcag ggggtgcggg gaggcgtggg
     4861 gacaacaacc atcaccgccg cattagcctg gtcattacaa atgttgggag aaaatgtcct
     4921 ggtggtcgat gcctgcccgg acaacttgtt gcgcctgtca tttaatgttg attttaccca
     4981 ccgtcagggc tgggccagag cgatgctgga tggccaggac tggcgtgacg ctgggttgcg
     5041 ctacacctcg cagctcgatt tgctgccttt tggtcagtta tccattgaag aacaagaaaa
     5101 tccacagcac tggcaaaccc ggctgagcga tatttgctcc ggcttacagc aactaaaagc
     5161 cagcgggcgt taccagtgga ttttaatcga cttaccgcgt gatgcctcgc agataaccca
     5221 ccagctgctg agtttgtgcg agcactcgct ggcaatcgtc aatgtggatg ccaactgcca
     5281 tatccgactg catcagcaag cgctgccgga tggcgcacat attttgatta atgacttccg
     5341 tattggcagt caggttcagg acgatattta ccagctttgg ttgcaaagcc agcgccgatt
     5401 actgccgatg ctcattcatc gtgatgaagc gatggctgaa tgcctggcgg ctaagcaacc
     5461 agtaggtgaa tatcgcagtg atgcgctggc ggctgaagag atactgacgc tggcgaactg
     5521 gtgcctgttg aactactccg ggctgaaaac gccagtcggg agtgcatcat gagtatcctg
     5581 acccggtggt tgcttatccc gccggtcaac gcgcggctta tcgggcgtta tcgcgattat
     5641 cgtcgtcacg gtgcgtcggc tttcagcgcg acgctcggct gtttctggat gatcctggcc
     5701 tggattttta ttccgctgga gcacccgcgc tggcagcgta ttcgcgcaga acataaaaac
     5761 ctgtatacgc atatcaacgc ctcgcgtccg cgtccgctgg acccggtccg ttatctcatt
     5821 caaacatgct ggttattgat cggtgcatcg cgcaaagaaa cgccgaaacc gcgcagcggg
     5881 cattttcagg tctgcaaaat attcgtggac gttaccatca atggatgaac gagctgcctg
     5941 agcgcgttag ccataaaaca cagcatctgg atgaaaaaaa agagctcggt catttgagtg
     6001 ccggggcgcg gcggttgatc ctcggtatca tcgtcacctt ctcgctgatt ctggcgttaa
     6061 tctgcgttac tcagccgttt aacccgctgg cgcagtttat cttcctgatg ctgctgtggg
     6121 gggtagcgct gatcgtacgg cggatgccgg ggcgcttctc ggcgctaatg ttgattgtgc
     6181 tgtcgctgac cgtttcttgc cgttatatct ggtggcgtta cacctctacg ctgaactggg
     6241 acgatccggt cagcctggtg tgcgggctta ttctgctctt cgctgaaacg tacgcgtgga
     6301 ttgtgctggt gctcggctac ttccaggtag tatggccgct gaatcgtcag ccggtgccat
     6361 tgccgaagga tatgtcgctg tggccgtcag tggatatctt tgtcccgact tacaacgaag
     6421 atctcaatgt ggtgaaaaat accatttacg cctcgctggg tatcgactgg ccgaaagaca
     6481 aactgaacat ctggatcctc gatgacggcg gcagggaaga gtttcgccag tttgcgcaaa
     6541 acgtgggggt gaaatatatc gcccgcacca ctcatgaaca tgcgaaagcg ggcaacatca
     6601 acaatgcgct gaaatatgcc aaaggcgagt tcgtgtcgat tttcgactgc gaccacgtac
     6661 caacgcgatc gttcctgcaa atgaccatgg gctggttcct gaaagagaaa cagctggcga
     6721 tgatgcagac tccacaccac ttcttctcgc cggacccgtt tgaacgcaac ctgggacgtt
     6781 ttcgtaaaac gccgaacgaa ggcacgctgt tctatggtct ggtgcaggat ggcaacgata
     6841 tgtgggacgc cactttcttc tgcggttcct gtgcggtgat tcgtcgtaag ccgctggatg
     6901 aaattggcgg cattgctgtc gaaaccgtga ctgaagatgc gcatacttct ctgcgtttgc
     6961 accgtcgtgg ctatacctcc gcgtatatgc gtattccgca ggcggcgggg ctggcgaccg
     7021 aaagtctgtc ggcgcatatc ggtcagcgta ttcgctgggc gcgcgggatg gtataaatct
     7081 tccgtctcga taacccgctc accggtaaag gactgaagtt tgctcagcgg ctgtgctacg
     7141 tcaacgccat gttccacttc ttgtcgggca ttccacggct gatcttcctg actgcgccgc
     7201 tggcgttcct gctgcttcat gcctacatca tctatgcgcc agcgttgatg atcgccctat
     7261 tcgtgctgcc gcatatgatc catgccagcc tgaccaactc gaagatccag ggcaaatatc
     7321 gccactcttt ctggagtgaa atctacgaaa cggtgctggc gtggtatatc gcaccaccga
     7381 cgctggtggc gctgattaac ccgcacaaag gcaaatttaa cgtcaccgcc aaaggtggac
     7441 tggtggaaga agagtacgtc gactgggtga tctcgcggcc ctacatcttc cttgttctac
     7501 tcaacctggt gggcgttgcg gtaggcatct ggcgctactt ctatggcccg ccaaccgaga
     7561 tgctcaccgt ggtcgtcagt atggtgtggg tgttctacaa cctgattgtt cttggcggcg
     7621 cagttgcggt atcggtagaa agcaaacagg tacgccgatc gaaccgcgtg gagatgacga
     7681 tgcccgcggc aattgcccgc gaagatggtc acctcttctc gtgtaccgtt caggatttct
     7741 ccgacggtgg tttggggatc aagatcaacg gtcaggcgca gattctggaa gggcagaaag
     7801 tgaatctgtt gcttaaacgc ggtcagcagg aatacgtctt cccgacccag gtggcgcgcg
     7861 tgatgggtaa tgaagttggg ctgaaattaa tgccgctcac cacccagcaa catatcgatt
     7921 ttgtgcagtg tacgtttgcc cgtgcggata catgggcgct ctggcaggac agctacccgg
     7981 aagataagcc gctggaaagt ctgctggata ttctgaagct cggcttccgt ggctaccgcc
     8041 atctggcgga gtttgcgcct tcttcggtga agggcatatt ccgtgtgctg acttctctgg
     8101 tttcctgggt tgtatcgttt attccgcgcc gcccggagcg gagcgaaacg gcacaaccat
     8161 cggatcaggc tttggctcaa caatgatgat aacgcgatga aaagaaaact attctggatt
     8221 tgtgcagtgg ctatggggat gagtgcgttc ccctctttca tgacgcaggc gacgccagca
     8281 acgcaaccac tgatcaatgc tgagccagct gtagccgccc agacggaaca aaatccgcag
     8341 gtggggcaag tgatgccagg cgagcaggga gctgatgccc ccatcgtggc gcagaacggt
     8401 ccttcgcgtg atgtgaagct gacctttgcg caaatcgcgc cgccgccggg cagcatggtg
     8461 ctacgtggta ttaacccgaa cggcagcatt gagtttggta tgcgcagcga tgaagtggtg
     8521 acgaaggcga tgctcaactt cgaatacacg ccatcgccat cgttactgcc tgtccagtcg
     8581 cagttaaagg tttatctcaa tgatgagctg atgggcgtgc tgccagtgac caaagaacag
     8641 ttgggtaaaa aaacgctggc gcaaatgccc attaatccac tgtttattac cgacttcaac
     8701 cgtgtgcggc tggagtttgt cggccattat caggacgtgt gcgaaaaccc ggccagcacc
     8761 acgctttggc tggatgttgg gcggagcagt ggactggatc tgacctatca gaccctgaat
     8821 gtgaagaatg acctgtcaca cttcccggtg ccattctttg acccgcgcga taaccgtact
     8881 aacaccttgc cgatggtctt tgcgggtgcg ccggatgttg ggctgcaaca agcatctgcc
     8941 attgtcgcct cgtggtttgg ttcgcgttct ggctggcgtg ggcagaactt cccggtgctc
     9001 tataaccaat tgccggatcg caatgccatt gtctttgcaa ccaacgacaa acggccggac
     9061 ttcctgcgcg atcatccggc ggtaaaagcc ccggtgattg agatgattaa ccatccgcag
     9121 aatccttacg tcaaactgct ggtggtgttt ggtcgtgacg acaaagacct gttgcaggca
     9181 gcgaaaggta tcgctcaggg taacattctg ttccgtggtg aaagcgtggt agtgaatgaa
     9241 gtgaaaccgc tgctaccgcg taagccgtac gatgcgccga actgggtacg taccgatcgt
     9301 ccggtcacat ttggcgaact gaaaacctat gaagaacagt tacaatccag cggccttgag
     9361 ccagcagcga ttaacgtttc gctaaacctg ccgccggatc tctacctgat gcgcagtacc
     9421 ggcattgata tggatattaa ttaccgctac accatgccgc cggtgaaaga cagttcgcgg
     9481 atggatatca gcctgaataa ccagttcctg caatccttca acctgagcag caaacaggag
     9541 gcgaaccgtc tgctgctgcg tattccggta ttacaaggtt tgctggatgg caaaacagat
     9601 gtctctattc cggcgctgaa actgggcgcg accaaccagc tgcgcttcga ctttgagtat
     9661 atgaacccga tgccaggcgg ttcggtagat aactgtatta ccttccagcc ggtgcagaat
     9721 catgtggtga ttggtgacga ttccaccatc gacttctcga agtattacca cttcatcccg
     9781 atgccggatc tacgcgcctt tgctaacgcg ggcttcccat tcagccggat ggcggatctg
     9841 tcgcaaacca tcaccgtgat gccgaaagcg cctaacgaag cacagatgga aacgttgctg
     9901 aatactgttg gttttatcgg cgcacagacg ggcttcccgg cgattaatct gacggtgacc
     9961 gatgatggca gcaccattca gggcaaagat gccgacatca tgatcatcgg tggtatcccg
    10021 gacaaactga aagacgataa gcagatcgac ctattggtgc aggcgaccga aagctgggtg
    10081 aaaacaccga tgcgccagac cccgttcccc ggcattgtgc cggacgagag cgatcgcgcg
    10141 gcagaaaccc agtcaacgct gacctcttcc ggtgcgatgg cggcggtgat tggcttccag
    10201 tcgccgtata acgaccagcg cagcgtgatt gcgctgttgg cagatagccc acgcggttat
    10261 gaaatgctta acgatgcggt gaacgatagc ggcaaacgcg ccaccatgtt cggttcggtc
    10321 gcggtgatcc gcgagtccgg tatcaacagc ctacgtgttg gcgacgttta ttacgtaggt
    10381 catctgccgt ggttcgagcg cttgtggtat gcgctggcaa accatccgat tctgctggcg
    10441 gtgctggcga ctatcagtgt gatattgctg gcatgggtac tgtggcgtct gctgcgaatt
    10501 attagtcgtc gtcgtcttaa cccggataac gagtaattga agatgaatgt gttgcgtagt
    10561 ggaatcgtga cgatgctgtt gttggctgcc tttagtgttc aggcagcctg tacctggcct
    10621 gcctgggagc agtttaaaaa ggattacatc agtcaggaag ggcgcgtcat cgaccccagc
    10681 gacgcgcgca aaatcaccac ctccgaaggg caaagttacg gtatgttctt tgccctggcg
    10741 gctaacgacc gtgcagcttt cgataatatt ctcgactgga cgcagaacaa tctcgctcag
    10801 ggttctttaa aagaacgttt gcccgcctgg ctgtggggca agaaagagaa cagtaagtgg
    10861 gaagtgctgg acagcaattc ggcctccgat ggtgatgtct ggatggcctg gtcgttgctg
    10921 gaggcggggc gtttgtggaa agagcagcgt tataccgaca tcggcagcgc gttgctaaaa
    10981 cgtatcgcgc gggaggaagt ggtgacggtg cctgggctgg gttccatgtt gttaccgggc
    11041 aaagtgggtt ttgctgagga taacagctgg cgttttaacc ccagctacat gccgccgacg
    11101 ctggcgcagt atttcacccg ctttggcgcg ccgtggacca cgctgcgcga aaccaatcaa
    11161 catttattgc tggaaaccgc cccgaaaggc ttttcgccag actgggtgcg ctatgagaaa
    11221 gacaaaggct ggcagctaaa agccgaaaaa acattgatca gcagctacga cgctatccgc
    11281 gtttacatgt gggtaggcat gatgcctgac agcgatccgc aaaaagcgcg gatgctcaac
    11341 cggtttaaac cgatggcgac attcactgag aaaaacggtt atccgccgga aaaagtggat
    11401 gtggctacgg ggaaagcgca gggtaaagga ccggtcggtt tttctgccgc catgctgcct
    11461 tttttacaaa accgcgatgc gcaggcagtt cagcgccagc gcgtggccga taactttccc
    11521 ggcagcgata cctattacaa ctatgtgctg accctgtttg gacaaggctg ggatcaacac
    11581 cgtttccgct tctcgacaaa aggtgagtta ttacctgact ggggccagga atgcgcaaat
    11641 tcacactaaa catattcacg ctttccctcg gtctggccgt catgccgatg gtcgaggcag
    11701 caccaaccat cagcagaccc tgcggatgga cagcggcaac actaatgccg tgcgcgggct
    11761 ggcgaatatt taccgccagc agtcgccaga aaaagctgaa gcgtttatcg cctcgctctc
    11821 tgccagccag cggcgcagca ttgatgatat cgaacgcagc ctgcaaaacg accgtctggc
    11881 acagcaggca gaggtactgg aaaaccaggg caaatgggcg caggcggcag cacttcagcg
    11941 gcaacgactg gcgctgggcc ccggcagcgt atggattact taccgactgg caggaagaag
    12001 ctgcgcggca atccaacgat ggcggcagca gtcagggctt cggctacacg gcgcgggcat
    12061 tacttgaacg acgtgttact tccaactggt ttgttggcac ggcaattgat atccagcagg
    12121 tgaaagatta cgcacccagc catttcctgc tctacgtacg ttattccgcc gccggatggc
    12181 agggtgacat ggatttaccg ccgcagccgc tgatacctta cgccgactgg taagttttca
    12241 gatagcgcct ctcttaatgc cgctgcgatc gggtatactc gggcggcaat ctgggatttc
    12301 cggggggaga caatttgcgc gtaagtcgct cgttaacaat caagcagatg gcaatggtgg
    12361 cagccgttgt cctggtgttc gtttttattt tttgcaccgt tttgctgttc catctggtcc
    12421 agcagaatcg ctataacacg gctacgcaac tggaaagcat tgctcgctct gtccgcgaac
    12481 ccttatcttc agctattttg aaaggcgata ttcccgaagc ggaagctatt cttgccagca
    12541 ttaaaccggc aggcgtggtc agccgtgccg atgtagtgct gcctaaccag ttccaggcgc
    12601 tgcgtaaaag ttttattcca gagcgtccgg tgccggtaat ggttactcgc ctgtttgagc
    12661 taccggttca aatctcgctg ggcgtctact cgctcgaacg tccggcaaat ccgcagccaa
    12721 tagcctatct ggtgctacag gcggattcct tccgtatgta taagttcgtg atgagcactc
    12781 tctcaacgtt agtgaccatt tacttacttt tgtcgcttat cctgaccgtc gccatcagct
    12841 ggtgcattaa ccgcctgatt ttgcatccgt tacgcaatat tgctcgcgaa cttaacgcca
    12901 tcccagccca ggagcttgtt ggtcaccaac tggcattacc gcgtctgcat caggacgatg
    12961 aaatcggtat gttggtgcgc agttacaacc tcaaccagca attgctgcag cgccattatg
    13021 aagaacagaa cgaaaatgcg atgcgcttcc cggtgtcgga tttgccgaac aaagccttgc
    13081 tgatggagat gctggagcag gttgtcgcgc gtaaacaaac caccgcgctg atgatcatca
    13141 cctgtgaaac cctgcgtgat actgcgggcg tgctgaaaga ggcgcaacga gaaattctgc
    13201 tgctgacgct ggtggaaaaa ctcaaatcgg tactgtcgcc acgtatgatc ctcgcgcaga
    13261 ttagcggtta tgactttgct gtcattgcca acggtgtaca ggaaccgtgg cacgcaatca
    13321 ctttgggtca gcaagtactc actatcatga gcgagcgcct gccgattgaa cgtattcaac
    13381 tccgtccgca ctgtagcatt ggcgtggcga tgttctacgg cgatctcacc gccgaacagc
    13441 tttacagtcg cgctatttct gcggcattta ccgctcgcca taaaggcaag aatcagattc
    13501 agttctttga tccgcagcag atggaagcag cccagaagcg gttgacggaa gagagcgata
    13561 tccttaatgc actggaaaat catcagtttg caatttggtt acagccacag gtcgagatga
    13621 ccagcggtaa actggtcagt gcggaagtgt tactgcgtat ccagcaaccg gatggcagtt
    13681 gggacctgcc ggatggctta atcgatcgca ttgagtgctg tgggctgatg gttaccgtcg
    13741 gtcactgggt gctggaagag tcctgtcgat tgcttgcagc ctggcaagag cgcggcatta
    13801 tgctgccctt gtcggtaaac ctctctgcgc tgcaactgat gcacccgaat atggtggcgg
    13861 atatgctgga actgttaacc cgctatcgca ttcagccggg aacactgatt ctggaagtga
    13921 cagaaagccg acgtattgac gaccctcatg ctgcggtggc aatcctccgt ccgctgcgca
    13981 atgccggagt tcgggtggcg ctggatgatt tcggcatggg ctacgcaggg ctgcgtcagc
    14041 tgcagcatat gaaatcgttg ccaatcgacg tactgaaaat cgacaaaatg tttgttgaag
    14101 gcttgccgga agatagcagc atgattgctg caattatcat gctggcgcag agcctgaact
    14161 tacaaatgat tgccgaaggc gtggagactg aagcacaacg agactggctg gcaaaagcgg
    14221 gcgttggtat tgcccagggc ttcctttttg ctcgcccact ccctattgaa atcttcgaag
    14281 agagttacct ggaagaaaag tagctacccc aaactgatta caaaacttta aaaagtgctg
    14341 gtttgtgcga gccagctcaa actttttaac ctttttgttt caattatgat ccaggtacat
    14401 ttctgtgatg ttgtctgggt gttattttaa ggccgcaggt acccccccca taaccttaca
    14461 agacctgtgg ttttactaaa ggacacccta tgaaaacctc tctgtttaaa agcctttact
    14521 ttcaggtcct gacagcgata gccattggta ttctccttgg ccatttctat cctgaaatag
    14581 gcgagcaaat gaaaccgctt ggcgacggct tcgttaagct cattaagatg atcatcgctc
    14641 ctgtcatctt ttgtaccgtc gtaacgggca ttgcgggcat ggaaagcatg aaggcggtcg
    14701 gtcgtaccgg cgcagtcgca ctgctttact ttgaaattgt cagtaccatc gcgctgatta
    14761 ttggtcttat catcgttaac gtcgtgcagc ctggtgccgg aatgaacgtc gatccggcaa
    14821 cgcttgatgc gaaagcggta gcggtttacg ccgatcaggc gaaagaccag ggcattgtcg
    14881 ccttcattat ggatgtcatc ccggcgagcg tcattggcgc atttgccagc ggtaacattc
    14941 tgcaggtgct gctgtttgcc gtactgtttg gttttgcgct ccaccgtctg ggcagcaaag
    15001 gccaactgat ttttaacgtg atcgaaagtt tctcgcaggt catcttcggc atcatcaata
    15061 tgatcatgcg tctggcacct attggtgcgc tcggggcaat ggcgtttacc atcggtaaat
    15121 acggcgtcgg cacactggtg caactggggc agctgattat ctgtttctac attacctgta
    15181 tcctgtttgt ggtgctggta ttgggttcaa tcgctaaagc gactggtttc agtatcttca
    15241 aatttatccg ctacatccgt gaagaactgc tgattgtact ggggacttca tcttccgagt
    15301 cggcgctgcc gcgtatgctc gacaagatgg agaaactcgg ctgccgtaaa tcggtggtgg
    15361 ggctggtcat cccgacaggc tactcgttta accttgatgg cacatcgata tacctgacaa
    15421 tggcggcggt gtttatcgcc caggccacta acagtcagat ggatatcgtc caccaaatca
    15481 cgctgttaat cgtgttgctg ctttcttcta aaggggcggc aggggtaacg ggtagtggct
    15541 ttatcgtgct ggcggcgacg ctctctgcgg tgggccattt gccggtagcg ggtctggcgc
    15601 tgatcctcgg tatcgaccgc tttatgtcag aagctcgtgc gctaactaac ctggtcggta
    15661 acggcgtagc gaccattgtc gttgctaagt gggtgaaaga actggaccac aaaaaactgg
    15721 acgatgtgct gaataatcgt gcgccggatg gcaaaacgca cgaattatcc tcttaatctc
    15781 accacttatg cccgcactcc cttccccgag tgcgggtgtt tgcgcataat tgacccctgt
    15841 gcctgaactt accacctggg tgagttcagg tttatttcac ttcttccgtg acattttcat
    15901 gttcttgcgg tctaacacga agtgttttta acgtcattta gactgaccga caacgggcag
    15961 tttttttatt accaggatag ttgatcaggg gttcacatgc agggcataaa aattcgactt
    16021 ttagcgggcg gtttgctgat gatggccact gctggctatg tgcaggcaga tgcgctccag
    16081 cctgatccag catggcaaca ggggacgctt tccaacggtt tacagtggca agtgctgacc
    16141 accccccagc gtcccagcga tcgtgttgaa attcgcctgc tggttaatac cggttcgctc
    16201 gccgaaagta cacaacagag cggttacagt cacgccatcc ctcgtattgc gctaacgcaa
    16261 agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc agcaggggat cgaccctaaa
    16321 cgcccgatgc cgccggtaat tgtctcttat gacaccacgc tgtttaatct gagtttgccc
    16381 aataaccgta acgatttgct gaaagaagcg ctctcttatc tggcaaatgc cactggcaaa
    16441 ttgaccatca caccagaaac catcaaccac gcgctgcaaa gtcaggacat ggtggcaacc
    16501 tggcctgccg atactaaaga gggctggtgg cgctatcgtc tgaaagggtc aaccttgtta
    16561 ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag cggaaaaaat taaagatttc
    16621 tatcagaaat ggtacacccc ggatgcaatg acgctactgg tggtgggaaa cgtggatgcg
    16681 cgctcggttg tcgaccaaat caacaaaacg tttggcgaac tgaaaggcaa acgtgaaacg
    16741 ccagctccgg tgccgacgct ttctccgctg cgtgcggaag cggtgagtat tatgactgac
    16801 gcggtgcgtc aggaccggtt atctatcatg tgggatacgc cgtggcagcc gattcgtgaa
    16861 tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc gcgaggcgct gttctggcat
    16921 gttcagcaag cgttaagcgc cagtgacagc aaagacatcg gtcttggatt tgactgccgt
    16981 gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat caccaaacga caagctgaac
    17041 agcaacctta atctggtggc gcgtgaactg gcgaaggttc gcgataaagg tctgccggaa
    17101 gaagagttca atgcgttagt ggcgcaaaag aaactggagc tgcagaaact gtttgccgcc
    17161 tatgcacggg ctgataccga tattctgatg ggccagcgga tgcgttcgtt gcaaaatcag
    17221 gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc aggatttcct taacagcctg
    17281 actgtggaga tgttaaatca ggatctgcgt cagcagttgt cgaatgatat ggcgttaata
    17341 ctgctgcagc cgaaaggcga gccggaattt aacatgaaag cgttgcaggc ggcctgggat
    17401 caaatcatgg ccccatctac cgccgctgcg accacctctg tcgccacgga tgacgtacat
    17461 cctgaagtga cggatattcc acctgcacag taatgcctga tggtgatgag ttagcacaac
    17521 catcaggtat ttatggatga gctggataga ggggtaacac tttatccagc cttttgcata
    17581 tgctgcgttt acgctggcat cgcctcacgc gggataatcg cgccgcgata ctgaataacg
    17641 gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg cactgccgcc cgtcaaacgt
    17701 accgccagat aaccggcgct gaaagagtcg ccagctgcgg tggtatcgat cactttttct
    17761 ttcggcagtt tcaccgccgg aacatcaact aacgcttcac cagcaatgga caccaggcaa
    17821 gaatccgccc cgcgtttcac caccacttct ttcacgcccg cgttatgggt gcgcgcaatg
    17881 acgtcttcca ccggctgttg accccacagc gcgtcttcgt cgtccagcgt caggaaggcg
    17941 atatccgtgc attccagcat ttgctggtac acctgctgtg tctcttcttt gctggcccac
    18001 aggcgcggac gatagttatt gtcgaaaatc acttttccgc ctttggcgcg gcattcgcgc
    18061 agcagggaaa gcagcttttc gcggctggtc gggcttaaga tcgccaggct aatcccgctc
    18121 aggtagagat aatcgaaatt cgccagctct tcgcaaatcg ccgcagactg ctcgctcgcc
    18181 agccagaatt tggcggcggc ttcgttacgc cagtagtaga acgtacgttc gccggtgctg
    18241 tcggtttcaa tgtagtaaag gcccggaaga cggttttcca tccgttgggt cagggaagta
    18301 tcaacgttct cgccgtgcca ggcgtccagc atctgctggc taaaactgtc cgttcccagc
    18361 gccgttacgt aatgaacggt taatgccgca ggatcgacct gacgggcgat atagacggaa
    18421 gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg cgcctttctc ggaaagctca
    18481 atcatgcatt cgccaatcac ggcaatcttt ttggacatag tcgtgaacct gatctgtaaa
    18541 aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa ccatattaaa acagtgttcc
    18601 attatttttt tgagccagaa cgtattcctg aaagattccg ttgtggagtg aggaaattta
    18661 ccccggccag gcggtaaagt tctgccctta cgcgccgata atctttgtcg agtccgggca
    18721 gcatcacttt taaacacagg acatctttga tgataaggca ggttatccag cgaataagca
    18781 accctgaagc aagcatcgag agcttgcagg aacggcgttt ttggttgcag tgtgagcgtg
    18841 cttacacctg gcagccgatc tatcaaacat gcgggcggtt aatggccgtg gagctattaa
    18901 cggtggtcac gcatcccttg aacccttcgc aacgcctgcc gccggatcgc tattttactg
    18961 aaatcaccgt cagccatcgg atggaggttg tgaaagagca gattgatttg ctggcgcaaa
    19021 aagccgactt ctttgtagag cacggcctgc tggcatcggt caatattgat ggccctacgc
    19081 tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccagat tgagcgtctt ccctggctgc
    19141 gtttcgaact ggtggagcat atccgtctgc cgaaagattc aacctttgcc tcgatgtgtg
    19201 aatttggccc gctgtggctg gatgattttg gtaccgggat ggcaaatttc tctgcactaa
    19261 gtgaagtgcg ttatgactac atcaaaatcg cgcgagaact gtttgtgatg ctgcgtcagt
    19321 cgccggaagg ccgcacactc ttttctcagc ttttacatct aatgaatcgc tattgtcgcg
    19381 gggtgattgt cgagggcgta gaaacaccgg aagagtggcg tgatgttcag aactcgcccg
    19441 cattcgccgc acaaggctgg tttctttcac gcccggcacc gatggaaacg ctgaatacgg
    19501 cggttctggc gctataagct gcctcatttt ccgccgggct ggactatctt taggactggc
    19561 acaggaagaa tatgaggaag cgaacgatga gcaaggcagg caaaataacc gctgcgattt
    19621 caggggcttt cttgttgttg attgtcgtgg cgatcatttt gattgcaact tttgactgga
    19681 atcgactcaa accgaccatc aaccagaaag tctctgcgga gttgaatcgt ccgttcgcta
    19741 tccgtggcaa tctgggcgtg gtgtgggagc ggcaaaaaca agaaactggc tggcgcagct
    19801 gggtgccgtg gcctcatgta catgcagaag acatcattct tggtaatcca ccggatattc
    19861 ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac gctggcaccg ctggcgctgc
    19921 tgaccaaaac ggtctggctg ccgtggatca agctcgaaaa gcccgacgcg cgcctgattc
    19981 gcctctctga aaagaacaat aactggacgt ttaatcttgc caacgatgac aacaaagacg
    20041 cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa tattcttttc gatcaagggc
    20101 ggatcgccat tgatgacaaa gtaagcaaag cggatctgga aatttttgtt gatccgttag
    20161 gcaagccgct gccattcagc gaagttactg gatcgaaagg taaagcggat aaagaaaagg
    20221 tgggcgatta catttttggc ctgaaggcgc agggacgtta taacggtgaa ccgctcacgg
    20281 gtacgggaaa aataggcggt atgctggcgc tgcgtggcga agggacgccg tttccggtac
    20341 aggctgattt ccgttcaggt aatacccgtg ttgcttttga tggcgtcgtg aatgacccaa
    20401 tgaagatggg cggtgtcgat ttacggctta aattttctgg cgattcactg ggtgatctct
    20461 atgaactgac gggcgttctg ctgcccgata ccccgccgtt tgaaacggat ggtcggctgg
    20521 tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta tcgcggtttt aatgggcgaa
    20581 ttggcgatag cgatatccac ggttctctgg tctacaccac cggaaagcca cgaccaaaac
    20641 tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga cctgggaccg ttgattggcg
    20701 ttgattccgg gaaaggggca gaaaagtcga aacggtctga acagaagaag ggcgaaaaaa
    20761 gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt cgaaaccgat aaatgggacg
    20821 ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga gcatggcagt agcctgccga
    20881 ttagcgatct ttctactcat atcatcctca aaaatgctga cctgcgcctg caaccgctga
    20941 aatttggcat ggcgggcggc agcattgcgg cgaatattca tctggaaggc gataaaaagc
    21001 cgatgcaggg gcgggcagat attcaggctc gtcgactgaa actgaaagaa ctgatgcccg
    21061 atgtggaact gatgcagaag acgctggggg aaatgaacgg tgacgcggaa ctacgcggta
    21121 gcggtaactc ggtggcggcg cttttaggca acagtaacgg caacctgaaa ctgttgatga
    21181 atgacgggct ggtgagccgc aacctgatgg agattgttgg gctgaatgtc ggcaactaca
    21241 ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa ctgcgcggcg gcgaatctga
    21301 atattgccaa cggcgtggcg cgcccgcaga tttttgcttt cgatactgag aacgcgttga
    21361 ttaatgttac cggcacggca agttttgctt cggaacagct ggatttgact attgatccgg
    21421 agagtaaagg gattcggatt atcacactgc gttcgccgct gtatgtgcgt gggacgttta
    21481 aaaatccgca ggctggggtg aaagccggac cgctgattgc ccgtggtgct gttgctgcgg
    21541 cactggcaac gctggtaaca ccggcggcgg cgttactggc actgatctca ccttccgaag
    21601 gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa gaagtgatga taattgccgg
    21661 atgtctgaca tccggcaatt aaacagattt agatttttgg caaagtgtgc tttgttcatg
    21721 ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct tgtgaattca atatattgta
    21781 tggaaattca gtcctgataa gcgtagcgca tcaggctttt cacacttatt acaacgactg
    21841 atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc aagccagcca tcgccgccag
    21901 atataacccc accgcaccta acccgtagtt agcctgcaac caggctgcga tatacggcgc
    21961 aacggaagcc ccgagaatcg acgatacgtt gtaagagaac gatgctccgg tgtaacgcac
    22021 ttctgtcgga aacagctctg gtaacagcgc acccatcggc ccgaaggtca gccccatcag
    22081 acttaacccc agcagcagga aggcaaaaac cagaatcggg ttgccagaac cgagcagcgg
    22141 gttaaaggcg aacagcgcga acaggatgat cagcgtggtg atgatcacca tgcttttacg
    22201 gcgaccaaag gcatcagcca gtaatccggc gactggcacc atcacgccaa aaccaataac
    22261 tgccatcatc agcatccaca acacttcgtt acgcggcagg ccaagcccaa ctggcgcggc
    22321 ggcggtactg aaggtcatag agtagaccgt catgatgtaa aacagcgtat aggttgccag
    22381 cataatgaac gtacccagta cggttacgcg aacatgtttg gtcagcagcg tacccagcgg
    22441 gatcttcacc tgttttttcg ctttagcgac tttctcaaac accggcgact catgcagcga
    22501 cacgcgaaca tacaggccga taatgaccag caccgccgag aagataaatg gcacacgcca
    22561 gccccagctc ataaactgct catcggtcag tagccaggaa agcagcaaga aagtgccgtt
    22621 ggcaaagaag aagccaatcg gtgcgcctag ctgtgggaag gagccataca gtgcacgttt
    22681 gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg ccccattcac cgcctaagcc
    22741 cagaccctga ccaaatcgag ccagcgccag cagcagtggg gcgaaaatac caatcgtggc
    22801 atagcccggc agcagaccaa tcaccacggt cgaaatcccc atcgttagca acgaggcgac
    22861 cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca aaaacggcag agccaatggg
    22921 gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt agcgttgctg ccgttggatc
    22981 gccctgcggg aagaagatat gcggaaacac aataacggcc gcagtggcgt aaatgtaaaa
    23041 gtcgaagaac tcaatggccg tgccaatgag agaggcgaca aggactttat tacgcgagtt
    23101 gatcggcgtg tattcttgtt cgtggtcgag tgttgtggct gttgcttgca taatcttttc
    23161 ttattttttg gctaacgaat agccatatta cgcacagcaa aagtgatatt tcaatgtgta
    23221 acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga taatgtttag gccagtgatt
    23281 tttcttctat gaaatgcttc acataatttt ataaatagtg gataaaactg gtttcaggtt
    23341 aaataaaagt taccaactgg atttatatgc tggggttatg agttggagtg aaattgtttg
    23401 ctcccaaata gatggctgaa gaggctctgg ggaagagttt caagaggtta tgtgcgcatt
    23461 atagccggat gcgatgctgg cgcatcttat ccggcctacg gggagccagg ccgaataaaa
    23521 tcgtatgccg gataaggcgt ttacgccgca tccggcctat tttaaggctg cgttttccct
    23581 ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc acgccgcgca aaacagcgtc
    23641 aaacgggcga agaagtagaa aaatgccatc agccccagca cggaaccaaa tgctgcgcca
    23701 gagggggatt tcatcagcga tggcagggtg taggtcatca cgattttaat cacttcaaaa
    23761 ccaatagcag ccagaaatgt tccgcgaatc agggcttttt tgcgcggacg gtggcgcggc
    23821 aaccgccaga agatccagaa gaaaagcaga tagttggcga agatggaaat cgccaggcca
    23881 atcaatcgcc acgtcggttt cagccactca atgctgttca ggtgcagggc gctaataatc
    23941 atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg tcacaatcag cgcaatcaac
    24001 aaaccaatca gtgaaataaa atcacgcaga tattttaccc agaacttctc ctgatcttgc
    24061 ggcgagcgtt cccagacatc gcgcgactgg gcacgaatcg cttcacgcag gttacccatc
    24121 cagttgatgc cggaataaag tgccaccgcc aggccgacaa gccctacagt cgtacgctgc
    24181 tgaacggcgg tgttgatggt gtttttcaac gtggcggcta gtgtcggatc gctgatgttt
    24241 tgcagaattt tgtcgaagat atcctgtagc aacatcggat gggaggccag cacaaagccc
    24301 cctgcggcaa acgacaccat caaaatcggg atcatcgaca aaaatgagaa ataggtgata
    24361 gccgcaccaa actggttacc cagccgatcg ttaaagcgtt ctgtcgcgcg aataaggtgc
    24421 gcaataaccg gttggcgttg gactttttcg gcagtggtgg tgacggtttc cagcgcctgg
    24481 ctaactttgc cgcctttctc gctattatcc aactgcttaa tcggctcgtg ctccagatcc
    24541 tgggtgggac gtttgatctc gttttcctgc gtcatccgct cttttgtcct tctgtcgtgg
    24601 tcattcttaa aaagtatagt cggtcagtcc aggtgctctt tcattacgcc agccagccac
    24661 tgcataaaca ggtttacacg ccgggaaagc tcccgacgct gcgggtaaac cagcgaaagg
    24721 gagagcggct cggcacggta gcctggcaac acttcaataa gccgcccggc acgcagggct
    24781 tcgcgcacgg caatgcgcgg gatctgaata atccccagac cggcaagacc ggcggcgaga
    24841 tagttttcgc tactgtttac cgtcaacatg ccgccagact taaaccactg gacgccatta
    24901 acgctggcaa cctcaaaacc taacggatgt acacccaggt gcggtgtata acgcactatt
    24961 gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa agcgcgtcag atagtgcgga
    25021 ctggcacagt tgaccatggt tagtttgccg aggggacggg cgataacgcc atcttccggt
    25081 aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa gaatatctac cggacggtta
    25141 tggctactca gttccagctc aattcccgga tgctgataga gaaattccga cagacgcggc
    25201 agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc gtaatttacc gctgatactg
    25261 gtggcatcct gttgaaacag accgtccagt tcgctgagat tactcaacac atcttttgcc
    25321 cgttgataat aggtcatgcc ttctggcgtg agtttgaccc gtcgcgtagt gcgctggaga
    25381 agctgggtgc caagttgatg ttccagtgcc tgtatctggc gcgaaacact tccctttggc
    25441 aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct ccgcgacttt gatgaacaac
    25501 tgcattgcgt gaattttatc catttctttg accgattgtt gttcacagcg aaacagtaac
    25561 gcgcatatcg cattgtttat tgttttttct aacatctaat gccgggtttc acaatgttgt
    25621 catgacgaat gtggcgattt ctcagcagga aaagggcgga atagctttat gagaaaggtg
    25681 aatcccctta aacagagcgt atttttcagt gattcccttt aaagtgaata gaggacgtat
    25741 tggctatttg tgcttatttt tgtctcattg ccatttgtta ttttggcttg tagtcgcgga
    25801 atttcatttt taattgcatg cgtactaacc accaatataa ggaaatcctc aaaattgttt
    25861 taaatagtgc cctcgacatg taatttcagg cgtttagctg taactattta tatcggtaac
    25921 tattttctac gcgtcatctc tgtagaatgg ccaccgccaa atggcgaatt cttatttttt
    25981 ctcgcgggta atacccttcc actacttaag tctttttaac gtattcaggt ggagattcag
    26041 ttatgcaaat agtcatgttt gacaggcagt caatatttat tcatggaata aaaatcagtt
    26101 tacagcagcg tattccagga gtgagtattc agggggtcag tcaggcagac gagttatggc
    26161 aaaagctgga aagttaccct gaagccttag ttatgctcga tggcgatcag gatggtgagt
    26221 tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc tgaggttaag gtgttaatca
    26281 cggcgacgga ttgcaataaa cggtggttac aggaagttat ccattttaat gtgctggcca
    26341 ttgtgcctcg cgattcaacc gtcgagacat ttgcgttggc ggtaaatagt gcggcgatgg
    26401 ggatgatgtt tctgcctgga gactggcgga cgacgccgga aaaggacatt aaagatctca
    26461 aatcgttaag cgcccgccag cgggagattt taaccatgtt agcggcgggc gaatcaaata
    26521 aggagatcgg cagagcgctg aatatcagta ccggaacggt aaaagcgcat ctggaatccc
    26581 tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc aatgatgtta aata
//
LOCUS       DAJTRD010000053        26552 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTRD010000053 DAJTRD010000000
VERSION     DAJTRD010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26552
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.053"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            347..640
                     /locus_tag="OO315_002403"
     CDS             347..640
                     /locus_tag="OO315_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5891286.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            712..1371
                     /locus_tag="OO315_002404"
     CDS             712..1371
                     /locus_tag="OO315_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5891287.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1463..1909
                     /locus_tag="OO315_002405"
     CDS             1463..1909
                     /locus_tag="OO315_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5891288.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="OO315_002406"
                     /pseudo
     CDS             complement(2116..3016)
                     /gene="hlyE"
                     /locus_tag="OO315_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3389..3808
                     /locus_tag="OO315_002407"
     CDS             3389..3808
                     /locus_tag="OO315_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5891289.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3808..5076
                     /gene="umuC"
                     /locus_tag="OO315_002408"
     CDS             3808..5076
                     /gene="umuC"
                     /locus_tag="OO315_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5891290.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="OO315_002409"
     CDS             complement(5122..5652)
                     /gene="dsbB"
                     /locus_tag="OO315_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5891291.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="OO315_002410"
     CDS             complement(5798..7339)
                     /gene="nhaB"
                     /locus_tag="OO315_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5891292.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7560..8279
                     /gene="fadR"
                     /locus_tag="OO315_002411"
     CDS             7560..8279
                     /gene="fadR"
                     /locus_tag="OO315_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5891293.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8331..9863)
                     /locus_tag="OO315_002412"
     CDS             complement(8331..9863)
                     /locus_tag="OO315_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5891294.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10193..11491
                     /gene="dadA"
                     /locus_tag="OO315_002413"
     CDS             10193..11491
                     /gene="dadA"
                     /locus_tag="OO315_002413"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5891295.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11501..12571
                     /gene="dadX"
                     /locus_tag="OO315_002414"
     CDS             11501..12571
                     /gene="dadX"
                     /locus_tag="OO315_002414"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5891296.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12739..14153)
                     /locus_tag="OO315_002415"
                     /pseudo
     CDS             complement(12739..14153)
                     /locus_tag="OO315_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14764..16500)
                     /locus_tag="OO315_002416"
     CDS             complement(14764..16500)
                     /locus_tag="OO315_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5891297.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16595..17509)
                     /gene="ldcA"
                     /locus_tag="OO315_002417"
     CDS             complement(16595..17509)
                     /gene="ldcA"
                     /locus_tag="OO315_002417"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5891298.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17609..18220
                     /gene="emtA"
                     /locus_tag="OO315_002418"
     CDS             17609..18220
                     /gene="emtA"
                     /locus_tag="OO315_002418"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5891299.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18222..18956)
                     /locus_tag="OO315_002419"
     CDS             complement(18222..18956)
                     /locus_tag="OO315_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5891300.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19157..19411
                     /locus_tag="OO315_002420"
     CDS             19157..19411
                     /locus_tag="OO315_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5891301.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19461..20921)
                     /locus_tag="OO315_002421"
     CDS             complement(19461..20921)
                     /locus_tag="OO315_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5891302.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20858..21415)
                     /locus_tag="OO315_002422"
     CDS             complement(20858..21415)
                     /locus_tag="OO315_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5891303.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21441..22295)
                     /locus_tag="OO315_002423"
     CDS             complement(21441..22295)
                     /locus_tag="OO315_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5891304.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22295..23104)
                     /locus_tag="OO315_002424"
     CDS             complement(22295..23104)
                     /locus_tag="OO315_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5891305.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23114..23872)
                     /locus_tag="OO315_002425"
     CDS             complement(23114..23872)
                     /locus_tag="OO315_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5891306.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23869..24849)
                     /locus_tag="OO315_002426"
                     /pseudo
     CDS             complement(23869..24849)
                     /locus_tag="OO315_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(24849..25871)
                     /locus_tag="OO315_002427"
     CDS             complement(24849..25871)
                     /locus_tag="OO315_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5891307.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
     gene            26221..>26552
                     /locus_tag="OO315_002428"
     CDS             26221..>26552
                     /locus_tag="OO315_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891308.1"
                     /translation="MLGVIQNLCCCRPDSPLADSKQRNQKLPQQDFEDFKNDVINVIN
                     NNEDIYFLRESNLLNAFIRTCKKRDDNSYGNMIFTLYFDNDIYGNRIIKASLNGGELQ
                     LLSTGCSN"
BASE COUNT         6671 a         6467 c         6855 g         6559 t
ORIGIN      
        1 atggcctttg ctgaactcaa cattgacatt cttacccgca gcaaagctcg tggaggttag
       61 cagtgcaagc agcaatacag ccttactgat gctcttgatt ttcattggta gtctcctgtt
      121 cagaatgagt acgatgaact gttataatat aacaatccct tacgggagaa ttccagtggc
      181 ttttctgcgt tcggtaaaat cccggcgcgg cattttttgc cggggggatt acttataaag
      241 gggcatggac tcaacaaacc gcagaaatct acagctggct ggcttgtggc gcgggtttca
      301 ttgatgttag cttatgccaa aacctggcat cttaaagagt aaaagtatgt tttgtgcgat
      361 ttatcgaagc agcaagcgtg accagaccta tttatatgtc gaaaaaaaag acgatttttc
      421 gcgtgttcct gaggaactga tgaaaggttt tggtcagcct cagttagcga tgattctgcc
      481 gttggatggg cggaagaaac tggtcaatgc tgatattgaa aaggttaaac tggcattaac
      541 cgagcaaggt tactatttgc agttaccgcc accacccgaa gatttgctga agcaacatct
      601 ttccgtcatg gggcagaaaa cagacgacac taacaaataa ccgatatccg gcggtggcat
      661 tatctttgtc ggcgcgggtt ttcatatcca cgataaggtg aggggaacgt tatgtatcaa
      721 catcacaact ggcaaggtgc gctgctggat tatccggtga gtaaagtagt ctgtgttggc
      781 agtaactatg ccaaacatat taaagagatg ggcagcgcag tgcccgaaga gccagtgctg
      841 tttattaaac cagaaacggc actgtgcgat ctgcggcagc cattggcgat cccatccgat
      901 ttcggttcag ttcatcatga agtcgaactg gcggtgttga ttggcgcgac gctgcgtcag
      961 gcaacggaag agcatgtccg caaagccatt gccggttacg gcgtggcgct tgatctgaca
     1021 ttgcgtgatg ttcagggaaa aatgaagaaa gccgggcagc cgtgggaaaa ggctaaagcg
     1081 tttgataact cttgtccgct ttccgggttt atccccgcgg cggaatttac cggcgatcca
     1141 caaaatacaa cgctgggcct gagcgtaaac ggcgaaaaac gccagcaagg tacgactgcg
     1201 gacatgatcc ataaaatcgt tccgttgatc gcttatatga gcaagttttt taccctcaag
     1261 gccggtgacg ttgtgttgac aggcacgcct gatggcgtcg gcccgttgca aagcggtgat
     1321 gagctgacag tcactttcga tgggcattct ttgacaactc gcgttttgta atactttttg
     1381 ccgccagaaa gcggcggcaa aacttgcatc gctgtgccag actggttata aggtgcgttt
     1441 taacgtaatg gcggaacacc tgatgagcga tgtacctttc tggcaaagta aaaccctgga
     1501 cgaaatgagc gatgcggaat gggagtcgtt gtgtgatggt tgcggtcagt gttgcttgca
     1561 taaactgatg gatgaagaca ccgacgaaat ctacttcact aacgtcgcct gtcgccagct
     1621 caatattaaa acctgtcaat gtcggaacta cgaacgtcgt ttcgagtttg aacccgactg
     1681 cattaaatta acccgtgaaa atctgccaac attcgaatgg ctgccaatga cctgcgctta
     1741 tcggttgctg gcggaaggta aagatttacc tgcgtggcat ccgctactta ctggttcgaa
     1801 agcggcaatg catggtgaac gtatctctgt gcgtcatatc gcagtgaaag aatcagaagt
     1861 gattgactgg caggatcata tcttaaataa acctgactgg gcgcagtgat aaatttaata
     1921 gatgctgatg ggggctttct taatatacgt ctggatgctc gccatttcgt agccctgttt
     1981 ttgaaagcgc tctgtcataa tgagagttac ccgatacctt attatcaaca ggtaactctc
     2041 aatctttaaa aaacgtctgt gtttttaaat gtgataaacc ttataccttg agtacatgga
     2101 gagaataatc gcttatcaga cttcaggtac ttcaaagagt gtctttttac cgtgtctttt
     2161 ctgatactca ttacaggtgt taatcatttt tttggccgct gcttttagca aagaaagcat
     2221 taaatcatca taatcaacat agaatctggt tgtttcagtt tccgttttta tctccccgat
     2281 ggcggctatt tcggtggtta atttcaattt ggcggcatcg atatctttat tcgcttgttt
     2341 aaccgtgtta gacagggtgg taaagaaact ctgcacagat tttaacttgt tcttcaattc
     2401 tggaatcagt ttcccttcaa ctacgcccgc agcaatagaa taggaaatga ttaaaccaaa
     2461 tggaccggcg acgacatcgg ctgcggcacc ggcatacgct tccttcctga ttttatctac
     2521 ctgtgactgg aaatagctgc ttttttctga aaaatcattg gttaactggc tatctaacgc
     2581 cagcagtttc ccggaagcgt tgttgaaact ttgtgagctt accagcaggg atttttgcgc
     2641 ttcattcagc ttcgtgatgc cgtcatccag taccttaatg aggggcggat gctttcttct
     2701 cattgtactc atcaaatagc aaaatatacg ctgcgagcaa ttgcgtcgca acaccacacc
     2761 attcatacac tgtttgggtt gcttcaaaat acttatcctg gctatccata agtaaggttt
     2821 taatatcgcc gactaaaacg gaggctgcct gtgaatactc ctgtttaaag cgacttaact
     2881 cttttatggt ttcatcaaag gtctgccagg ggatgacctg atcgagatat ttattataaa
     2941 gatctaatgc tccatctgcg gtttcgattg cgtttttaac tacttctacc gttttatctg
     3001 caacgatttc agtcataatc attcgcctct ttaaatatat aaattgtaat gaaactcctg
     3061 ttttacaact attaataaat tttacttcat ctaattcata gttagccggg cgggatgcgt
     3121 caatgtcttt atttctatta atatgataaa tatcaaacaa tgtttaatgt cattatggcg
     3181 aatgcttcta ttctattttt ttgccgggtg atatttttca tttctgctgg atgagcgtcg
     3241 tcgccagaag gccacgtgag cacaggataa gagaacgaaa aatcagcagt ctatgcagcg
     3301 acaaatattg atagcctgaa tcagtattga tctgctggca agaacagacc actgtatata
     3361 aaaacagtat aacttcaggc agattattat gttgtttatc aagcctgcgg atctccgcga
     3421 aattgtgact tttccgctat ttagcgatct tgttcagtgt ggctttcctt caccggcagc
     3481 agattacgtt gaacagcgca tcgatctgaa tcaactgttg atccagcatc ccagcgcgac
     3541 ttacttcgtc aaagcaagtg gtgattctat gattgatggt ggaattagtg acggtgattt
     3601 actgattgtc gatagtgcta ttaccgccag ccatggtgat attgtcatcg ctgctgttga
     3661 cggcgagttt acggtgaaaa aattgcaact acgcccgacg gtacagctta ttcccatgaa
     3721 cagtgcgtac tcgcccatta ccatcagtag cgaagatacg ctggatgtct ttggtgtggt
     3781 gatccacgtc gttaaggcga tgcgctgatg tttgccctct gtgatgtaaa cgcgttttat
     3841 gccagctgtg agacggtgtt tcgccctgat ttatggggta aaccggtggt tgtgctatcg
     3901 aataatgacg gttgcgttat cgcccgaaac gctgaggcaa aggcgctggg cgttaaaatg
     3961 ggcgatccct ggttcaaaca aaaagatctg tttcgtcgct gtggtgtggt ttgctttagc
     4021 agcaattatg agctttacgc agacatgagc aatcgggtga tgtcgacgct ggaagaacta
     4081 tcgccccgcg tcgagattta cagtattgat gaagcattct gcgatctgac aggtgtgcgt
     4141 aattgtcgcg atctgactga ttttggcaga gaaattcgcg caacggtgct acaacgtacc
     4201 catcttactg ttggtgtggg gatcgcccag accaaaacgt tggctaagct tgccaatcat
     4261 gcggcaaaaa aatggcagcg gcagacgggt ggggtggtgg atttatcaaa tctggaacgc
     4321 cagcgtaaat taatgtctgc tctccccgtg gatgacgtct gggggattgg acggcggatc
     4381 agcaaaaaac tggacgcgat ggggatcaaa accgttctcg atttggcgga tacagatatc
     4441 cggtttatcc gtaaacattt taatgtcatg ctcgaaagaa cggtgcgtga actgcgcggc
     4501 gaaccctgtt tgcaactgga agagtttgca ccgacgaagc aggaaattat ctgttcccgc
     4561 tcgtttggtg aacgcatcac ggattatacg tcgatgcggc aggccatttg tagttacgct
     4621 gcccgggcgg cggaaaaact tcgcagtgag catcaatatt gtcggtttat ctccacattt
     4681 attaagacgt caccatttgc gctcaatgaa ccttattacg gcaatagcgc gtcggtaaaa
     4741 ctgctgacgc ccactcagga cagcagggat atcattaacg ctgctacgcg atctctggat
     4801 gccatctggc aagcgggcca tcgctaccaa aaagcgggcg tgatgctggg ggatttcttc
     4861 agtcagggag tcgcgcagct caatttattc gatgacaacg caccgcgccc cgggagtgag
     4921 caattgatgg cggtaatgga tactctgaat gctaaagagg gcagaggaac actctatttt
     4981 gccgggcagg ggatccaaca acaatggcag atgaagcgag ctatgctttc accacgttat
     5041 acaacgcgaa gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagcgct
     5101 cccgcaggag cgccgaatgg attagcgacc gaacagatca cgttttttcg ctttaaacgg
     5161 ctgggaaatc accaccagca ccgcgacaat caggtaagcg ataaaaatac cgagcagcca
     5221 ctgcggcatt tccataccta aaaaatccca ctgacgctcg gcgcaatcgc cagaggcgac
     5281 aaacacttgc ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc
     5341 acaggtggca aatggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac
     5401 accgcggaac gcactataca accagataac catcgctaca taacgcagcg gagttttcgg
     5461 ggcgatcgcg ccaatcagcg cagcacccag aacgccgaat aacgcgcagc gttcataaat
     5521 acagagcacg caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag
     5581 tgccagagca gtaaacgcca tcaacagcca cgcgccccgg ccttgtgaac attggttcaa
     5641 aaatcgcaac ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca
     5701 ttcgctgcgc caccaggagg cagcaaaaat aggacgcaat tggacaatta tcgggcgaaa
     5761 aggcaaaccg ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca
     5821 tttgcataaa ccattcggtt acaggggcaa gcgtaaactc gacgcagagc aagccgacga
     5881 gtgtcagtac gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa
     5941 tcaatggcgc gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag
     6001 cgacagaggg cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca
     6061 acgtgatagc gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga
     6121 agacgttatc cgaaatggat gacagcagac cgttgaaaat atagaacagc gacagctgag
     6181 catgttcaga tgcctgcaac acaaactgaa taatcggcga aaacagttgt tggtcgataa
     6241 tcaccgcgac taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga
     6301 aggctttacc gatagcatgt tcatcggtga caccggtcaa tgatgttgcc agaataatga
     6361 ctgacaaacc aatcaagcca acttccgcca gatgcaacgc cagcgcagtc accagccaga
     6421 cgccaataat cgcctggaca atcagacgga ttttatcctg acgggtacgc tggtggcggc
     6481 tttgatcgtc aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc
     6541 caaaccaacg cagcttctct accagcaggc aggttaacag gccacaaatc agaaccggaa
     6601 cggtcaccgg cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga
     6661 tgatcaggtt ctgtggttcg cccaccatgg tcattacgcc gcctaatgcg gtgccgacac
     6721 cggcatgcat catcaggcta cgcagaaagc cacggaactg ttccagaacc acgttgtaat
     6781 gcttgtcgat atgactatcg tcttgcaggt cggtgtcttc ggtacgggaa gaggctacgc
     6841 gatgataaat accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg
     6901 catcgaggaa cgcggagagg aacgctgccg ccacgcaaaa agagagcgac agcagcattt
     6961 tggagcgaat gcttaacagc aaacgggtaa atatgaacag caacagctgt ttcataaaat
     7021 agatacccgc caccataaac atcaataaca gcaagacttc aagatttgcc gccacctctt
     7081 cacggacgtg ttccgcgctg gtcatgccga tgaataccgc ttcgatagcc aacagaccac
     7141 cggggagtag cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca
     7201 gcaaccagcc cgcgacgaaa gggctgatga ggaaaattaa cgggtttacg attaagaaaa
     7261 tgatgagggc gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc
     7321 ggccccagga gatctccatg atggtttccc ttaccttaca aataatcaat gatgttttta
     7381 tgtttaaacg caaagcttaa cggtcaggca ggagtgaggc aagtcttgat agtcaagggg
     7441 aaagagatgc ggaaaatgaa gccttgatcc ctttttcttc tttttgtctg ctatcagcgt
     7501 agttagccct ctggtatgat gagtccaact ttgttttgct gtgttatgga aatctcacta
     7561 tggtcattaa ggcgcaaagc ccggcgggtt tcgcggaaga gtacattatt gaaagtatct
     7621 ggaataaccg cttccctccc gggactattt tgcccgcaga acgtgaactt tcagaattaa
     7681 ttggcgtaac gcgtactacg ttacgtgaag tgttacagcg tctggcacga gatggctggt
     7741 tgaccattca acatggcaag ccgacgaagg tgaataattt ctgggaaact tccggtttaa
     7801 atatccttga aacactggcg cgactggatc acgaaagtgt gccgcagctt attgataatt
     7861 tgctgtcggt gcgtaccaat atttccacta tttttattcg caccgcgttt cgtcagcatc
     7921 ccgataaagc gcaggaagtg ctggctaccg ctaatgaagt ggccgatcac gccgatgcct
     7981 ttgccgagct ggattacaac atattccgcg gcctggcgtt tgcttccggc aacccgattt
     8041 acggtctgat ccttaacggg atgaaagggc tgtatacgcg cattggtcgt cactatttcg
     8101 ccaatccgga agcgcgcagt ctggcgctgg gcttctacca caaactgtcg gcgttgtgca
     8161 gtgaaggcgc gcacgatcag gtgtacgaaa cagtgcgtcg ctatgggcat gagagtggcg
     8221 agatttggca ccggatgcag aaaaatctgc cgggtgattt agccattcag gggcgataat
     8281 cccttccgtt taaagagcaa ccccctcaaa cgaggggttt tttgttgttt ttacagattt
     8341 cccattcttg gcgggcaacg ttccagcaac tcgatgctgc cgtcttcgtt ttgctgttcg
     8401 agcatcacat caaatcccca caggcgatgc acatgcttca ggacttcttt gcgcccccga
     8461 tccagcggtg cgcgattatg tggaatataa cgcagcgtca gcgaacggtc gccgcgcaaa
     8521 tccacgttcc agatctgaat attcggctcc agattactta agttatattg cgacgataac
     8581 cggttacgga tctcccgata accttcttca ttatgaatag cggaaatctc cagataatta
     8641 tgccgatcat catccagcac ggtgaagaag cggaaatcac gcatcacttt cggtgacaga
     8701 aactggctga taaaactctc atctttgaaa tcacgcatcg cgaaatgcag cgtttccagc
     8761 cagtcggaac cggcgatatc cgggaaccag tatttgtctt cttccgttgg cgactgacaa
     8821 atccgtttaa tatcctggaa catggcgaac ccgagggcat acgggttgat gccgctgtac
     8881 cacgggctgt tatagggggg ctggaagacc acattggtgt ggctgtgcaa aaactccagc
     8941 ataaaacgtt ccgttacttt cccttcatca tacagatggt taaggatggt atagtgccag
     9001 aaggtcgcac agccttcgtt catcacctga gtctgttttt gcggataaaa atactggctc
     9061 accttacgca caatacgcag gatttcacgc tgccatgatt ccagcagtgg cgcatttttc
     9121 tccataaaat agagcaggtt ttcttgtggt tcggacggat agcggcgcgc ttcagcaacc
     9181 gttttctctt cctcgcgctt cggcagggta cgccagagca tattgacctg actttgtaga
     9241 tactcttcgc gacttttctg ccgggctttc tcttcttgca gcgagatttt ttgcgggcgt
     9301 ttgtaccggt ccacgccgta gttcatcagc gcgtggcacg agtccagaag ccgttctact
     9361 tcatcaacgc cataacgttc ttcgcactcg gtaatatatt tacgggcaaa aatcagataa
     9421 tcgacaatcg aactggcgtc ggtccaactg cggaataagc aattgttttt aaagaaagag
     9481 ttatgtccat agcaggcgtg tgccatcacc agcgcctgca tagtaatggt gttctcttcc
     9541 atcaagtaag cgatacacgg gttagagtta atgacgattt cataggccag cccttgctga
     9601 ccgtgcttat acagccgttc agtctcgata aactttttac cgaatgacca gtgcggatag
     9661 ttaattggca tgccgacgct ggaataggca tccatcatct gttctgaggt tatcacttca
     9721 atctggtgcg ggtaggtatc cagccggtag agtttcgcca cccggtctat ctctgccaga
     9781 taaacatcca gcaggtcgaa cgtccagtcg ggtccatcgc tcaaacgtgt ggtgtcctta
     9841 ttcatagaat cgatcgtcgc catacgcgca cctcattgtt gtcggcgctc tctgtgtgga
     9901 gcacctcatt tcaagcatag aacacctgtt aaaaaccgcg tcgccggaga atttttttct
     9961 ttgcgatttc ttattatcag agtgccacta atccgcttct gaacggaatt ttatgctgga
    10021 taaaaagggc gttcagcagg agatactaaa gacgccatat tgccgcagag tcagggagat
    10081 gtgagtcagc tcaccataaa aaagccgtat gttgaataat attttcaact gagttatcaa
    10141 gatgtaatta gattattatt cttttactgt atctaccgtt atcggagtgg ctatgcgagt
    10201 tgtcatactg ggaagtggtg tggtaggcgt tgccagcgcc tggtacttaa atcaggcagg
    10261 acatgaggtc accgtcattg atcgggagcc gggggccgcc ctggaaacca gtgccgcgaa
    10321 tgccgggcaa atctcccccg gatatgctgc accgtgggca gcaccaggtg tgcctttaaa
    10381 agcgattaaa tggatgttcc agcgccatgc gccgctggcg gttcgtctcg acggtacgca
    10441 gtttcagttg aaatggatgt ggcaaatgtt acgtaactgc gacaccagcc actacatgga
    10501 aaacaaaggg cggatggtgc gtctggcgga atacagccgt gattgcctga aagcattgcg
    10561 cgccgaaacc aatattcagt atgaagggcg tcagggtggg acgctgcaac tgttccgtac
    10621 cgaacaacag tatgaaaatg cgacccgcga tatcgccgtg ctggaagatg ccggcgtacc
    10681 gtatcagctg ctggaatcca gccgcctggc ggaagtggat cccgcgctgg cagaagtagc
    10741 gcacaaactg acgggcggcc tgcagttacc caatgatgaa accggagact gccagctgtt
    10801 tacccagaat ctggcgcgga tggcagagca ggcgggggtt aaattccgct ttaatacgcc
    10861 cgttgaccaa ctgctttgcg acggcgagca aatctacggc gtgaagtttg gcgatgaagt
    10921 gattaaggcc gatgcgtatg tgatggcgtt tggttcttac tcgacggcga tgctcaaagg
    10981 cattgttgat attccggtgt atccgctgaa aggctactcg ctgactattc caattgcgca
    11041 ggaagatggt gcgccagtat ccaccattct tgatgaaacc tacaaaatcg ccattacccg
    11101 tttcgataac cgcattcgtg ttggcggaat ggcggaaatt gttggtttta acaccgagct
    11161 gttgcaaccg cgtcgtgaaa cgctggaaat ggtggttcgc gatctctatc cacgcggcgg
    11221 tcatgtcgag caggcgactt tctggacggg tttgcgcccg atgacgccag acggtacgcc
    11281 ggttgtcggg agtacacgct ttaaaaatct gtggctgaat accggtcacg gcacgctcgg
    11341 ctggacgatg gcttgcggtt ccggtcagtt gttaagcgat ctgctctctg gtcgcacgcc
    11401 agcgatccca tatgaggatc taagcgtagc gcgctacagc cgtggattta cgccatcacg
    11461 tccgggccat ttacatggcg cacacagcta aggaaacgag atgacccgtc cgatacaggc
    11521 cagcctcgat ctgcaggcat taaaacagaa tctgtccatt gtccgccagg ccgcgccgca
    11581 cgcgcgcgtc tggtcggtgg taaaagcgaa cgcttacggg catggtattg agcgtatctg
    11641 gagcgcgctc ggggccaccg atggctttgc attacttaac ctggaagagg caataacgtt
    11701 acgtgagcgc ggctggaagg ggccgatcct gatgctggaa ggatttttcc atgctcagga
    11761 tctggagatt tatgaccagc accgcctgac cacctgcgta cacagcaact ggcagctcaa
    11821 agcactgcaa aatgcgcggc taaaagcacc gttggatatt tatcttaaag tgaacagtgg
    11881 gatgaatcgg ttgggcttcc agtccgatcg cgtgcttacc gtctggcagc agttgcgggc
    11941 aatggcgaat gttggcgaaa tgaccctgat gtcgcatttt gctgaagcgg aacatcctga
    12001 tggaatttcc ggcgcgatgg cgcgtattga gcaggcggcg gaggggctgg agtgtcggcg
    12061 ttcgttgtcc aattcggcgg cgactctgtg gcacccggaa gcgcattttg actgggttcg
    12121 gcctggcatt attttgtatg gcgcttcgcc gtccggtcag tggcgtgata tcgccaatac
    12181 cggattacgt ccggtgatga cgctaagcag tgagattatt ggtgtccaga cgctaaaagc
    12241 gggcgagcgt gtgggctacg gcggtcgcta tactgcgcgc gatgaacagc gaatcggcat
    12301 tgtcgccgca ggatacgccg acggttatcc gcgccatgcg cctaccggtg ctcctgtttt
    12361 agtggacggc gtgcgcacca tgacggtggg gaccgtctcg atggatatgc tggcggttga
    12421 tttaacgcct tgcccgcagg cggggattgg tacgccggtt gagctgtggg gcaaggagat
    12481 caaaattgat gatgtcgccg ccgctgccgg aacggtgggc tatgagttga tgtgcgcact
    12541 ggcgttacgc gtcccggttg tgacagtgta acttgttgta agccggatcg gaggcagcgt
    12601 cttctggttg cgaaaaaatc atccatccgg ctggtcagca actgtagttg ttaatgtgac
    12661 agagccattg cccatgatag tgtccattaa aagaatggac actatttccc cggaacctga
    12721 actcgccgca caggcgttct acataaaacg cttacgcttc attgttgact cggagtcgac
    12781 ttcagataaa tcacgcttca cccttgatgc agattcgggt tcatgttcag attgttgatt
    12841 aacaggctgc gagtcctgtt gtctttccag actcaacagg cccaactgtt gatccaggag
    12901 ctccaggtcc tcctgtacct tcacgcgctc caggagttcc gggtcatcca ggaactccag
    12961 atccataagg tcctccagct cctcgagtcc ttccaggtca acctagcctt ccttgtgcaa
    13021 aaagtccaaa aggtccttca ggccctgctg ttttttcagg ccctcgacgg ctttgagcag
    13081 cgtcggtaaa ttgtctttgt cccacgggtt accattacag tcaaacacct tcaggctctg
    13141 gggctgcggc acctgtaccg ttttaaactt gttttctcca cagtagtaaa ttattagttt
    13201 gggcggcagc cgtggtgaag agatcaaatc gttactagaa cagtcaaata ctttcaggcg
    13261 agtcaagttc tccagcatct catcaggtat tgagttaata ttgttgccgg cacaggatag
    13321 tttatacagg tactggggca tcgacggcgg taaaaattgc aagaagtttt cagaacagtt
    13381 aatcactctc aggttagtcg acagctcagg cagccagata atactgtttt catagcactt
    13441 cagtaccatc agttttgagg gctgcacctt cggtaacaag atcaagacgt tatctgtaca
    13501 gtcaagcacc agcagccact ttggcatcac cttcggtaac gcggtcaaca cgtttttact
    13561 gcagtaaaat tcaatcagat gtggaggcaa attctcaggg agtgaagtca atcccaggtt
    13621 gctaagattg agcatactcc gtcttgtaat gagacattcc ttcattctgc gcaatgcttc
    13681 tgcccggttt tcaatccggc ttgctggcag gttttcttcc aggatatttt ccttgatttc
    13741 ttccagactt tctttcaggt cttcttgggg aatttctgac tgtttgtttt gctggttttc
    13801 ttccaggatt tcgtacaggt ttttttgcag gttttcttgc aaaccatcct gctgtatagc
    13861 ccattcgcac catttggcga ccatcatgaa ctcgttatgt tgctgattca gacgagtccc
    13921 caacgtgatg atggtattgt tgtccacctg tatgttcact ttgcccgcga tactgaggtc
    13981 aatgatctgc cagggggcat caccccagct gaatttttgc gggctgagat acttcatgtt
    14041 ctgcaaaatt tgattcttga gaagagccgc cctggcgggc gtcgtacgcc tgggttaacg
    14101 gaagatgggg gaatggcggg ggcgaatggg cagatgtctt taaagctagc cataatgcta
    14161 cgtttccttg aaataaatat cctctggcag cgccggagac gtgaatgtgg tcaccgcaaa
    14221 ggagccacag gacgtcattt gcgctgatat cactactcat cgttgacttt tttcctggtc
    14281 agaaatgccg tgtcgggtct ggtacatgct gtattacggc ctaggtagct ctgacgtatt
    14341 aaagaaaata cgagagtgaa cgacccggaa ataatcgtta ttaagcaaag gaataactat
    14401 aagaaatcgc tcatgcatcc ggtgctacag gcggatgaaa cgcctattgt gtgggctatg
    14461 tcagtgcggc aggttgcgtg ggggaatatg ttcaggcgat gtcaggcagg ggcccctgat
    14521 ggttgaccgg taatgcgggg ctggttttta ttacctgtta cagtcggaaa aagcccttac
    14581 gctgactttc ttgagccggt gttttgagtt cattagccag agcctgcaat ggtttgtcat
    14641 ccataattta accttcttct gatgttggga tgagcatatc aaaatcagga aatgacaagt
    14701 ttattacagg ctcaggctgc gttcagtaaa gcatcgcatc cggcgataga tgctgcatta
    14761 aatttaagat tcagcttcct cttcagccac ccgaacgcca atcttcaaca cttcattatc
    14821 ttctttctcg gccaccgtcc agatcatccc ggcaaactct acctggtcac cgacaaccgg
    14881 tgccgcgcct agcaactgct ggacaatttc acccagcgtt tgctgtttat cacgatattc
    14941 tcgcccgtct tccaggccat atatcagcgc aacatcagca tatttggcgc tggcttcgag
    15001 aatgaagtca ccaaagaagc gttgatccag cgcgaccggt ggcgactggc tgaacagttt
    15061 accgagcgcc gggagatcgc gttcccgacc aattacacac aatacatcgc cttcgcgcag
    15121 tcgggtgctg ccggtaggat gaagcaactg gttatcacga aacagggctg caatacgcgt
    15181 ctcttttggc atatgcaaat cgcgcagtgc cgcacctaca caccatttat cagcactcaa
    15241 ttgataaaca aactgctccc acggattttc cggatgaata tccaggccaa cgcgtgacac
    15301 cggacgtccg acgggcggaa ccaccacttt ggcttttttc gccgcccacg agagtgatgt
    15361 tccctgcaag agcagtgaaa ccagaaccac aaagaaggcg acattaaaga acagtcgcgc
    15421 attctccagc cccgccatca tcgggaacac ggctaggatg attggcaccg cgccgcgtaa
    15481 tcccacccag ctgataaaca cgcgctcacg cagattgaaa ccgcggaaag ggagcaatcc
    15541 agcaaatacc gaaaggggac gggcgaagaa tatcatccat gccgacaaaa tgagcgccgg
    15601 aatggcaata ggcagcagat cgcttgggtt aaccaatagc cccagcacca ggaacatggc
    15661 gatttgcgcc agccaggcga ggccgtcgaa attttgcagg atgccgtagc ggttgcgaat
    15721 cggccgatta cccagcagaa aaccgcacag ataaacagcc agaataccgc tgccttccag
    15781 cgccgtggtt aaagcaaaaa ttaaaatacc gccgcttaat gccagcaatg gatataatcc
    15841 ggcaggcagg gcgatgcgat taatcatttg cagcagtaaa taaccaccgc caagaccgat
    15901 gacaatcccg aggccaaatt gctgcagaat atcgaccacg aacatccaac tgacactgct
    15961 ttcatgctgc tggatcattg caatcagggt aatcgtcaga aagaccgcca ttggatcatt
    16021 actgccggat tcaatttcca gcgtcgagcc aacacgttcg ttaagcccct taccacccag
    16081 cagagaaaag accgctgcag catcggtgga gccgacgata gcgccgatta ataagccttc
    16141 aatcaaatca agattaaaca gccacgccgc catcatgccg gttaaaccag aggtgataag
    16201 tacgcccagc gtcgccagcg acagcgccgg tcctaacgcc acacgaaagg aactggcctg
    16261 agtgcgcatc ccgccgtcga gcaaaataat cgccagcgcc aggttactta ccatgtaggc
    16321 gaaggggtaa ttatcaaacg ggataccgcc gacgccatcg actcccgcca gcatgccgat
    16381 cgccagaaaa ataaccagaa taggaatgcc aagacgggaa gaaaatgaac taagtaatat
    16441 actgctggtg acaaggatgg atcctaagat gaaaaggcta attattgttg tggcatccaa
    16501 tggtcggtta ctcctgatta cgctgtctct tatataaacc ctaccatatt agcggcagag
    16561 acagcgtttt acttagccct gaagcgtgat ttttttacat tttaagaaca ggatgaccgc
    16621 taatcgtcaa ctgagcgcct tcccgggtgt tattcagaat ggcatgtgcg cccaacggca
    16681 gcgtaaccgt gcgttgttca tgaccaaaat cgaggccggt aatgagtgga atcgacaggc
    16741 gggaacgcaa aaacgcgtac actgactcca ggttgtaacc tgcgtcataa tcattgggcg
    16801 tgctgccgct aaagctaccg agaataatcg ccttctgacg tggcaaaatt cccgcatgat
    16861 agagctgcaa cagcatacgt tcgacgcgga aagggtgcac gttaatatct tccagcacca
    16921 gaataccgtt ctcaattttt ggcatccacg gtgtaccaat cagtgaaatc agcatcgcaa
    16981 gattgcctcc ccacaacgtg ccttcggtct gacatgtcgg gccttcccct tgccattcaa
    17041 tggtgaaggt ttcattgcgt aacgccagcc agaaatggtg ctcggtaaag gcgttcagtt
    17101 catccgcacc aaagtttgct accagcatgg ggccgctaaa ggtgatgaca ttgccttgcg
    17161 ccagaagacc gcactgaatg gcggtaaaat cgctatgtcc gcaaatgagt aacgggtcat
    17221 gttgttggcg agccaccagt gcctgccagt caatatctgc cagtaaacga ctggcaccgt
    17281 aaccgccgcg tacagccagc acgatggtgt tgggggtagt cagtctggca agggaattga
    17341 gatcttccag acgctccgtt tccgtacccg caaaacgctc acaacgacgg gcaataacct
    17401 cgacgttatt gacttgatgc cccgcgtcgg taaggcgctg gataccacgc agcgcggcgt
    17461 gctgtttaat gctgtaaccc gatggggcaa ttaagtgaaa cagagacatg gcaattcctt
    17521 gctgacaaca gaaacgaaat gtatatcatg ccgcttaggt gtgccgttgt cacctcaacg
    17581 gcgattccag gctataagga tagaagaagt gaaattgaga tggtttgcct ttttgattgt
    17641 gttattagcg ggttgttcat caaagcatga ctatacgaac ccgccgtgga acgcgaaagt
    17701 tccggtgcaa cgtgcgatgc agtggatgcc aataagccag aaagccggtg cagcctgggg
    17761 cgtcgatcca caattgatca cggcgattat cgctatcgaa tcgggtggta atccgaacgc
    17821 ggtgagtaaa tcgaatgcca ttggtttgat gcagttaaaa gcttcaacct ccggacgtga
    17881 tgtttatcgc cgtatgggct ggagtggtga gccgacgacc agcgagctga aaaatccaga
    17941 gcgtaatatt tcaatggggg cggcttacct gaatattctg gaaaccggcc cgctggcagg
    18001 cattgaagat ccgaaggtac tgcaatatgc gctggtggtg tcatacgcta acggggcagg
    18061 tgcgctgcta cggactttct cgtcagatcg gaaaaaggcg atcagcaaaa tcaacgattt
    18121 ggatgctgac gagttcctcg atcacgtagc gcgaaatcat cctgcgccgc aggctccgcg
    18181 ctatatctac aaacttgagc aggcactgga cgcgatgtaa atcagtcgcg cactttgtcc
    18241 gctttttccc gggcttctcg ctcgagagag aaaataatcc gctgtaattg ccgctccacc
    18301 gtcgggctga cgttaagaaa acggaagctc agacggggag tggtgatggt ttcattcttg
    18361 ccatcaatca ctttgcgctc gctggtggag attaactggg cgtcaaagtg aaaaacaccc
    18421 cattgcccca tgttgacttc aatttgagcg aagcgcatac cttcttgtaa ttcggcaggc
    18481 tttgctgttt ccagtaatgc gcccatgccg cctaacgaca aatcatacaa gcggaaacgt
    18541 aacgtactgt tatccgccag tttggtctgg caaaaataag gcggatggag tggggcggag
    18601 atgcggaaat atcggcgtcg ttgtacaaac cataaggtgg gaggcggtac ggtaataaat
    18661 gccggaagct gcaagtattc actctgctgt agttgttcaa cagtaaactc gactttcgca
    18721 ccctgagttt cggcggtaat ggtaatgtgc tgtgccttta gcacggcgat gttgtcttcg
    18781 gcttgactgc cgaaatccag caccagttta tccggggtta ttgccagtat tttgctgatc
    18841 agctgcccgc cattccaact gagacgcaaa ggaattgcgg ctttgtgcaa atcgcgtaac
    18901 acgcccagga cggctaacgg attttgtttc aggaactgct catggtaatg actcacgcgg
    18961 aaaaactcct gactgacaaa ctgtctttgg tttatcggtc atagttaaca aaacttaata
    19021 caaatgcgtg aatatttttt acatgttgtt cttaaatcag ccgacaaacg cccagcatcg
    19081 atcccgccct tgcctatact tagagcgttg acgtaagcat ttcttgcgac cgattcatcg
    19141 aaacgagggc ctgaacatgg gaattattgc ctggattatt tttggcctga tagccggcat
    19201 tatcggcaag ctaatcatgc cggggcgtga tggtggtgga tttttcctga cctgtattct
    19261 cgggatagtc ggtgcggtgg tcggcggctg gctggcgacc atgtttggca ttgacggctc
    19321 catcagcggt tttaatttgc acagcttcct ggtggcggtg gtgggcgcta tcctggtgct
    19381 gggcgtattc cgcctcctgc gaagagaata agattttcat aaggcggata gcgatacaga
    19441 tgccgctatc cgctttcaca tcagaacgta tactcgacac ctgcccagaa attgcgacct
    19501 tcttcaataa acccttcact gtaggcatat tgggtatcaa acaggttatt gaccgacgca
    19561 ttgacgctga agccatgacc taaggtgtaa tcggctcgaa tgtgggtcac cgcaaaaccg
    19621 gcggcttttt gtgaaccgtc actgttgctg tagctggagg aacgcgcctc ttccgacagc
    19681 gttacgctta acggctccca tggtttgagt gtcatccatg cggtcattgt ctgcgttgga
    19741 agatcggtta tcttgccgat gtctttgcgt ttggcgtcag cgtggatcag agcgtagctc
    19801 aatcctacat ccagtatatt gctgattttc cccttaatac cggcatccag accgctgtaa
    19861 tccaccgtgc cgctgttctg attttgaatg gtatcggcat tgatattgtg cgagaggatg
    19921 gcatcactca cccggttata gtaaacgctg acctcaaatc cccagtcgcg cgtgaaggca
    19981 ccattccagg ttaaatccac cccgcgagcg cgttccggct tgagctgcgg gttaacaatc
    20041 gctatctggt tatacgcagg tttggatgtg gtatagcgtt ctttcagcgt cggaaagcgt
    20101 gtgcggtcat agtacgaaag cgccagagtg tcttcattgg caaagtggta tttccccatc
    20161 acctgccagt taaaagctga ctgattgttg tcgtcataat gggtgatgct gccatctttc
    20221 tcgtgttttt tcgcttctac gctatcgcgc cagtcatagc tgattcccgc cacgacatcg
    20281 acattatcag cagcagccca ttgatattca ctggcgagcg accaggtacg atcttcatag
    20341 cgatcgtaag cggcgtgcgg cgcacctttt tcccggtgta cgtcatcttt ccagttaacg
    20401 gcaaacgaca gcagatcgtt ttcacgcaca tcggctgcca gttgtaatcc ggcaccgtcg
    20461 ctgtaatcgg aataatggct gtagctgcct tttttatttt tcaaatcagc cagcgagttg
    20521 tacatcatca gcgtattttc aaaggtgtcg cgatacagcc gacttttcag ggtaaaacgg
    20581 tcgtttagtt gggtcgctcc ctgataataa aaactttctt tgtcatactc tggccactgc
    20641 cagtagcgtg atttttgacc acaatttccg ctgtatggcg ggttatcttt ttcaccatcc
    20701 tgcttaatgt aagtcagcgt gtattcatcg ttttcgcgcg gtgtaaaacc gagcttcaca
    20761 atgccgcgtt tatcatcagc cgatgaatta atcatcttgc cgtgtttgcc tgcaatatca
    20821 ttatttacac catgcggcag gccgagaaaa tcctgcttta gctggctacc gctgacttgc
    20881 aaatacccaa gatcgctgct ggcggcaaat gaagcatgca tatcgtaggc attgtcctga
    20941 ctacggctcc atccctggcg atatcccaga cttgcttctt cagggtggtg atattaatgg
    21001 ctccgcccat ctgattaggc ccatgaagca gcgacgaata ccctttggaa acttcgactg
    21061 cccccagatt gttggtcaga atccgcgcca gatcgaggtt gccgtcatag gggacataaa
    21121 tgggcacacc gtcgaaatag accggcacct gccgactatc aaagccacga acttttacct
    21181 gttcttcgtt gcggctgcct gacttttgca gcaccacgcc ggggacgaca cttaatgcct
    21241 gagcgacatt ctgtttatcc aaggccttca tggtgggttg atcgagaacg gtggtcgtcg
    21301 tcgatgatac cggactggac catacggtca gcgtgtcgct ctcctgagcc gctacggcct
    21361 gctgggtaaa ggcaagtgat aatgctgtgc agagataacg ttttttgagt ctcatagaat
    21421 aaattccgtt taacaaagaa ttaaatagag gctgtgggtt gcagactgac tttaatgtca
    21481 gctggtgccg cgtaataagg cgcggaggta ataaaaaggc gaatgccgca gtcgaggtaa
    21541 tttttgagtg tcgtcagatt aattccgccg gtcagagcca gcgtgcagtg aggagccagc
    21601 gacggcgcta tttgcgcaat ttcagttgcc tgttgcggac taaatttgtc gagctgaagt
    21661 acgtctggtt gtgcgcgtaa cgcggcgata gcctctttcg gcgtgtcggc ttcaacaaca
    21721 attttcttct cgggtgcgtg gcgacgtaac tgagcgattg cgcctgacca gccctgattg
    21781 tcatgaagaa aatggcggtg gttggcaaac aataatatgg tttccgcaca tccggcacga
    21841 tgaattagtc ctccggcagc cagaattgcc tgcgaagcca gtaagcgggt gcccggaatt
    21901 gcttttcggg tgcaggcgat attgccgtca ggataacgtt cacgaagcaa tgccagcatt
    21961 tgagcgagat aatcagaaac accgcaactc cactccagca cattctggac tgccttccat
    22021 ccctgatgaa gtgctgcggc attaccctgc gcacggatta gccgttgacc cgcgttcgct
    22081 tgtgaaccgt cgctgaccgc gtcatcaata gttaatccca gcgtagttaa catcttacac
    22141 gcgacagaaa cgccgctgac gcagccgccc tgacggtgaa aaaacgctat atagccaggc
    22201 tggtgtccaa tatttaatgc tcgcgtggtc aggtcacctc cctggatatc ttccagcagt
    22261 aacgcatcga tttgtgcctg agaaaggaaa atcatcacag tgcctcctgc ggtacgctat
    22321 cccaccagat gagcgcccag tcgcggctgg caggggcgag ggcacgagca tcttgttgtt
    22381 gataccagtg atataaccgc tcacgctcat cgtcgttaag cgcgccaaga ctccagctga
    22441 cgttctgctc aaaacgttcc caggtgctgt tatcctgttg gcaattttgt ccacggataa
    22501 aatcaacgtg ggcataaatt cccatctgat acaggacgtt gagcgcaaag atatagttgg
    22561 gtaattcgat cacttcccga ccagccgccc gctgaatggc tggagagacg aacgaggtgc
    22621 tgaccagatg agttgtatag acgcgaagcc gtgcctgatt attcagtttg ctcattgcat
    22681 ggcgcatatc agccaccaga gtggagcggg aagccacggc aatatcgcag cgtggcaggt
    22741 cactccagtc ctcttcccag gcgcgctgga tccagtggac attgtcagcc ttcagtgcgg
    22801 cggcgcggcg ggcggcaacg ttcagcatcc cctgactgta atcaacgcca taaaccgtag
    22861 tgagtttgtc agccagcgcc agcgatacgg tgcctggtcc gcagcccata tcgaataagg
    22921 tctgagcgcc ttgcaggtca attttagcga tcagttgttg caggtagctg tccgttggcg
    22981 aggcgcaatt ttccgccatt ttctccgccc gttgatccca gtgatcgggc gttttttctg
    23041 tgcggtgagc cagtttgagt tgctgcagat aaaggtctgc aaaatcaata tcatcaatga
    23101 gcatgattgt tccttagcgg ctaattgcag agaggtgatg atgaatttgg tcggcagaaa
    23161 cccgatacag ggccgccagt ttgttcgttg ttaactgttc ggtcggtaag ccttgtgtta
    23221 ctcgtccatc tggctctact tggataatgc tgtcggcaat ggcgttggca tggagcgtat
    23281 ggtgagtcga cattaacatt gtcatgccgt tatttttgag ctgcgccagc gtgtccagaa
    23341 gttggatctg atgaccgaaa tcgaggctgg aggcgggctc atccagtaac aacaagcgcg
    23401 gttgttggac gagtgcacgg gcgattaaag ccagctgacg ttcaccgccg ctaagcgtat
    23461 tccagcgtcg tgtcgccaga tgcaaaatgc ccagcttttc cagttgctcc gtggctttca
    23521 ggcgttcttg cttaccggga acggaaaaag cgcctatggt gggggccagg cccatcaaca
    23581 ccatatccag cacactgaaa gcaaaaatac cgtcgtgcgc ctgcggcacc caggcaatag
    23641 cctgtgcgcg ctggcgatgg ctgagtgtct gcacagagag gctatccagt aggatctcgc
    23701 cttttaataa cggtataacg ccaagaatgg agcgcatgag tgtggttttg ccgcaaccat
    23761 tcgcgccaag cagacaacaa attgttcctt gcgacagggt gaaggacacg tcgcgtagca
    23821 ctggatggcg ggagtaaccc agagacgcat gacgaacggc aagcaaagtc actggcgacc
    23881 tccgcgtaac aacaaaagca ggaaaaacgg tgcgccaaca aaagcggtca gaatgccaag
    23941 tgggatttct gttgttccaa tgctgcgcgc cagcgtatcg gtaagcagga gcagaatagc
    24001 gccagtacac attgccatag gcaacagctg ctgatggtta tgaccggtca acaagcgggc
    24061 aatgtgcgga acgaccagac cgatccaacc aattatgccc gctattgcta ccgtgctggc
    24121 ggtgattagt gtggcgcaga caatcaatcc aaagcgcaga cgcgtcacgt tcagtcccag
    24181 cgaacgggct tcgtcgtcgg aaagcgtgag taaattcatg cgccagcgga gaaaaaacag
    24241 gggcaaggag cctatgagaa tgatcggtgc agcatagcag agatcgcgta aggtcacggt
    24301 ggacagtccc cccagcaatc agaaggtaat tgacggtaac tgggtgtaag gatcggccag
    24361 cgttttaatt agcgatatcc ccgctccgca caacgttcct aacgcaatgc ctaccagcac
    24421 cagggttaag atcggatcgt ggcgggtcac ccgacgcgta atcaaccaga cgcccgcgac
    24481 caccatcagc ccgccgcaaa atgcgtacag ctggatgtaa acaatgggta atccccacag
    24541 gattgccgcg caggcaccaa gtccggcacc ggcggagacg cctaaaatat ccggtgaaac
    24601 taagggatta cgcaacattc cctgataggt ggttccggct cctgccagcg ctgcgcccag
    24661 taacagcgca gcaagaatac gcggcacgcg gatttgccag aagacaattt gttcctgcac
    24721 agactcattt tcttgacgaa gaattagcgc cagcagttgc tgcatatcga gatgataagc
    24781 cccacttacg gcggcaaaag tgatactgat agctgccagc agcaaaatac atccgtttac
    24841 gattctcatt agctcgccac caatttttga tagtcggcat cactgagtga acaatgccag
    24901 aacagttggg cgtaatgctg catgtcactt ttaaactggc gattgatggc gggatccagc
    24961 caggcgtgaa gtctgcgtaa tcccagaaga cggttgattc ccggcggggc atcgagccag
    25021 ccaaagggca agccacttaa aaataagatg cgttgttccg caacggcttt cacgccctgc
    25081 cagagaggat cacgacgaat aaaatctgca gtaacggcct cctgaaccag aataatatcc
    25141 ggctgccagc gcaggagatt ttccatggaa acctgtgtca ggccgtggcg atcggctatt
    25201 tgcgcgacgt tgtgcaaacc taataattcc gccgcctcgg tatgcaacga accctgtaag
    25261 cccgtttcca gccctcgggg accgcgcgca gcataaaagc ggaggttagc ggcgggtgag
    25321 gtggcgaatg cctgagcttc accaacgaag tggctggcga gattggcttg ttctgcggcg
    25381 cggtgctctt cgcctaacgt tttgccaagc gttgttaact gttctgctga ttgcgccagt
    25441 tttccgttaa gcaataacca ggggatttgt gtctgttcgc taacctgccg tgcctgggag
    25501 atcaaggttt catccgtatt gccgcaatca acgaccaaat cgggatgtaa cgccataagc
    25561 ccttccagcg atagtgtgct ggcgcgcccg gcgagtcgtc ccagcctggg taactggcga
    25621 atgtgctctg gcaatggaat taatgcctga cgggcaaaat caaaagagga aaaaccgacc
    25681 attttttctg gcgcgacagc cagcaagagc aaatcggcgg gcgcgcctgc gctgacgatg
    25741 cgtgtaatgt tttgggcttc gggaagagat tgcgaagcaa aacgattaac tgtttgtgaa
    25801 aaggacggta gagactgcag gagcagggta gatgcaagtg cctgcgcaaa cgtccgtctg
    25861 gttatgggca tggtgaacat cctgaaaagt aaaatcgata tatactttca tatatatcga
    25921 taaaaatcat gttcaatatc aaagtttagc tattgaaaac atgaagatgt gacgacttcc
    25981 gcaagcctgt tgaccctctc tatcattaaa aatgtattta tcaggtggct aatattattt
    26041 aaatcattta aggctaaatt atttaaatat gaatgacatt attgtttttt tatcgtccac
    26101 ggttacgcga cgttgaagtt acttgtttta taatgagcga tttttgatat ctcgccgaga
    26161 aaattatcag cattctctcg tttcagtgtc gtgaatatac tgttaacgga ggtgaaaaga
    26221 atgctggggg ttatacaaaa cctatgttgt tgtcgtccgg attcaccgct tgctgatagt
    26281 aagcagcgta accagaagtt accgcaacaa gactttgagg attttaagaa cgacgttatt
    26341 aatgtcatta acaacaatga agatatttat tttcttagag agagtaattt actgaatgcg
    26401 tttattcgga cgtgcaaaaa aagagatgat aacagttacg gaaatatgat atttacgctt
    26461 tattttgata acgatatcta tgggaacaga attatcaaag catcgctgaa cggcggagag
    26521 ttgcagttat taagcacagg gtgtagcaat ga
//
LOCUS       DAJTRD010000054        26416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTRD010000054 DAJTRD010000000
VERSION     DAJTRD010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.054"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..436)
                     /locus_tag="OO315_002429"
     CDS             complement(<1..436)
                     /locus_tag="OO315_002429"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891309.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFI"
     gene            complement(582..1145)
                     /locus_tag="OO315_002430"
     CDS             complement(582..1145)
                     /locus_tag="OO315_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891310.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(1352..2290)
                     /locus_tag="OO315_002431"
                     /pseudo
     CDS             complement(1352..2290)
                     /locus_tag="OO315_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(2353..2727)
                     /locus_tag="OO315_002432"
     CDS             complement(2353..2727)
                     /locus_tag="OO315_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5891311.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(2731..3879)
                     /gene="wzy"
                     /locus_tag="OO315_002433"
     CDS             complement(2731..3879)
                     /gene="wzy"
                     /locus_tag="OO315_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5891312.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(3899..4804)
                     /gene="rfbG"
                     /locus_tag="OO315_002434"
     CDS             complement(3899..4804)
                     /gene="rfbG"
                     /locus_tag="OO315_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5891313.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(4797..5687)
                     /locus_tag="OO315_002435"
     CDS             complement(4797..5687)
                     /locus_tag="OO315_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5891314.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(5717..6973)
                     /gene="wzx"
                     /locus_tag="OO315_002436"
     CDS             complement(5717..6973)
                     /gene="wzx"
                     /locus_tag="OO315_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5891315.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(6989..7534)
                     /gene="rfbC"
                     /locus_tag="OO315_002437"
     CDS             complement(6989..7534)
                     /gene="rfbC"
                     /locus_tag="OO315_002437"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5891316.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(7539..8417)
                     /gene="rfbA"
                     /locus_tag="OO315_002438"
     CDS             complement(7539..8417)
                     /gene="rfbA"
                     /locus_tag="OO315_002438"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5891317.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(8476..9375)
                     /gene="rfbD"
                     /locus_tag="OO315_002439"
     CDS             complement(8476..9375)
                     /gene="rfbD"
                     /locus_tag="OO315_002439"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5891318.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(9375..10460)
                     /gene="rfbB"
                     /locus_tag="OO315_002440"
     CDS             complement(9375..10460)
                     /gene="rfbB"
                     /locus_tag="OO315_002440"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5891319.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(10833..11726)
                     /gene="galF"
                     /locus_tag="OO315_002441"
     CDS             complement(10833..11726)
                     /gene="galF"
                     /locus_tag="OO315_002441"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5891320.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(11901..13295)
                     /gene="wcaM"
                     /locus_tag="OO315_002442"
     CDS             complement(11901..13295)
                     /gene="wcaM"
                     /locus_tag="OO315_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5891321.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(13306..14526)
                     /gene="wcaL"
                     /locus_tag="OO315_002443"
     CDS             complement(13306..14526)
                     /gene="wcaL"
                     /locus_tag="OO315_002443"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5891322.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(14523..15803)
                     /gene="wcaK"
                     /locus_tag="OO315_002444"
     CDS             complement(14523..15803)
                     /gene="wcaK"
                     /locus_tag="OO315_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5891323.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(15983..17461)
                     /gene="wzxC"
                     /locus_tag="OO315_002445"
     CDS             complement(15983..17461)
                     /gene="wzxC"
                     /locus_tag="OO315_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5891324.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(17463..18857)
                     /gene="wcaJ"
                     /locus_tag="OO315_002446"
     CDS             complement(17463..18857)
                     /gene="wcaJ"
                     /locus_tag="OO315_002446"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5891325.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(18912..20282)
                     /gene="cpsG"
                     /locus_tag="OO315_002447"
                     /gene_synonym="manB"
     CDS             complement(18912..20282)
                     /gene="cpsG"
                     /locus_tag="OO315_002447"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5891326.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(20475..21911)
                     /gene="cpsB"
                     /locus_tag="OO315_002448"
     CDS             complement(20475..21911)
                     /gene="cpsB"
                     /locus_tag="OO315_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5891327.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(21914..23137)
                     /gene="wcaI"
                     /locus_tag="OO315_002449"
     CDS             complement(21914..23137)
                     /gene="wcaI"
                     /locus_tag="OO315_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5891328.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(23134..23613)
                     /gene="gmm"
                     /locus_tag="OO315_002450"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(23134..23613)
                     /gene="gmm"
                     /locus_tag="OO315_002450"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5891329.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(23613..24581)
                     /gene="fcl"
                     /locus_tag="OO315_002451"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(23613..24581)
                     /gene="fcl"
                     /locus_tag="OO315_002451"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5891330.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(24584..25705)
                     /gene="gmd"
                     /locus_tag="OO315_002452"
     CDS             complement(24584..25705)
                     /gene="gmd"
                     /locus_tag="OO315_002452"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5891331.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(25731..26279)
                     /gene="wcaF"
                     /locus_tag="OO315_002453"
     CDS             complement(25731..26279)
                     /gene="wcaF"
                     /locus_tag="OO315_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5891332.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(26295..>26416)
                     /locus_tag="OO315_002454"
     CDS             complement(26295..>26416)
                     /locus_tag="OO315_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HCR5891333.1"
                     /translation="ADAKKVQRQILHVPGFWAELSWHLRQRTTGKTKALYNKV"
BASE COUNT         7216 a         6579 c         5647 g         6974 t
ORIGIN      
        1 ggataaaata tcgtcccaac ttgtaatttg taatgttgat atcttttatt aaacttgcaa
       61 ttgagatcgc aacctgcaat actgacaatg ggtaaatggg tgataaatac ctgtcacctt
      121 gcgtaacaga aaaccaaaat aagacatatg ttaaaacaat cgagaaaaat acccaaagta
      181 ttttattatt cctaagtgta aagacagaca gtatagcata aaaaacaaca tcactgccaa
      241 ctttattaat tgcttgttgc aaatctaatg tggcaggact tataccatgt cgagcttgtt
      301 ctgctgtttg aacttgtaga tcaatttcat tccataggta gtgaccaaat atatttcctc
      361 cagcaggact aaaaggatca ccagagatcc aaaaactacg gacataccaa taaatgccac
      421 caagaattgt tgccaagcta aaaataagaa atgtctgtat gtttctcttt ttcgctatca
      481 atataacaaa tatgatggct ccatatatgg caccaaatag ttttacgcca caagcaattc
      541 caataaaaaa accacataaa aaaattaatt cattttgtgt tttattgttt tctgagcgaa
      601 tatatataag aatactggct gcagcaaaac aaagatagcg ttaaatcaat atatgcataa
      661 ccaagatgat ctttaagtgg attgatagta aatagtagga ttgtagcaat aatgcctgga
      721 taataagttt tcgtaatttc ccatgaacag ccaacaagtc cacaggctat tacaaaaaaa
      781 cagatatttg ctaaaaactg agctaacctt acatctccta actgtaaacc taaagccatt
      841 aaaagatctg cattttgcgg gaacagagga aaacgcaaat attgttcaac aacaattgca
      901 tggtgctcaa ctaatgaacg agccaaaggt aagtgataca tagtatcatc ccaatcaccg
      961 ggagctcgca gggacgataa caatgtaacc acaaaaatca aaacaagaaa taaaataaca
     1021 aacagcgtac tgaactcaat attatttatt gccagccatc taaattgctg acgtttaata
     1081 atgcttaaaa cccaacacac cgtaaaaatt aaaaagatag caatcttatt aaattgcccg
     1141 accatgccca gaaagtaaat tatcaaaata tttaaagaca tccctatgca taggcaataa
     1201 aaaataaaga aaatttgtta agtcctgcca ccccctcctt aaaaaaagat gacaatacaa
     1261 tattaccaat gacaaaaaat gtcattccaa aagcaaaaca atacacaaca tacaaataca
     1321 acggcaattc aatcatagta aaatactcta tttacattat cagtttatag tttttttcag
     1381 aaagataaaa taatgccttc atttcacgtc tagttgttgt atttgaatgc aaaataatgc
     1441 cacatacaaa tgccattatt gacgataaca tgatggaggc agttaaaaag gcagtcggaa
     1501 atcttctcac caaacctgta tcaatataat caacaatgat gggcaatcct aaaattatag
     1561 acaaagcagc aagtaaaacg ccgataatac taaaaaagta tagtggccgt tcaagcgagt
     1621 aaagtttaat tattgttttc agaatcttga acccatcact ccaagttgat agtttgctca
     1681 cactcccttc agatcgctcg ccatattttg tattaacctc accatacttc atacgtaatt
     1741 ctaaagcatg aattgtaagc tcagtttcga tttcaaatcc ccttgataga catggaaaag
     1801 atttaacata gcgtctggaa aatacacgat accccgacaa catatcagta aaattccctt
     1861 taaagatagt ctgaacagta ccagtcaaca atttatttcc tcagcgatgc ccttttctgt
     1921 atgtattttg atctccattc tcttgacgac atcctacaac catatccaat tgatttttta
     1981 tgagatggtt aatcatttct ggagcagaac ttgcatcata agtgtcatct ccatcaacca
     2041 ttagataaat atccgcatct acatctgaga acatacgtct tacgacattc cccttgcctt
     2101 tcagaggaac agagtgaact gttgctccag catcctctgc cacttgtgct gtactgtcag
     2161 tcgaattatt atcaaataca tatatagaag cagttggaat attcgttttg aaatcattta
     2221 tgacttttgc tattgccttc tgctcattat aacaaggaat aattacagcg atattaaaag
     2281 agtgatacat aagcccctca tataaactat ttagctaatg tgcaagtaat tgtgttttta
     2341 acaaatcgtt tactaacgaa aaatgaaata ttttgatgcg caatatccta aaaccaaact
     2401 aaaaagagaa aaggtgaaca atgtaaatat tggagataaa gctaagagat caaataaaac
     2461 gccgaataaa taactcatcg cccccataaa aaatataaat ataaaatatc tagccttggt
     2521 tgcagatact ttaaatgagc atcttgcatt tgcaaaaaaa gaaaaggttg ctgcacatat
     2581 aaaaccaaca atgttacttc ggccttgacc aaaagctaaa ttattataac acgcataaaa
     2641 taccccccaa tgtaaagcag tattgacaat tccaattaaa aaataactaa aaaaagaact
     2701 ggtcaataac ttccctattt ttaacatcct ttattttgct ccagaagtga ggttattact
     2761 aatttggata ttttctatag aaaataccca taaaactaca acgcctataa taaaaattcc
     2821 catgggtgta aaaagaaatg gattagttat agaaacggta atactgaaaa aaagtattaa
     2881 cgacatcatt aatgatgttt ttgtactatt taaaaaatag cctttcaaaa agataagcaa
     2941 cataggaact aatgatataa atacaccaat aacgcctgtt ttctgaagta tctcaagagg
     3001 tactatttca atccttaatc gatctaatat aaatgaacca aatcctcttc caaacaagaa
     3061 cgtcgctaaa tcaacatttt tataataaaa atataaatca ttaaatctaa cagagtcaga
     3121 atcttcactg cgtgatccga ccatgtaaag cataattata aatagaacgc tgcccaatat
     3181 tattataata gtatttaaat atagcttatg taatttaaaa ctggctaata acagtgataa
     3241 tgttgtaaaa agtaatacac ctcttgattc tgtcaataat atcgcaagca ctattatgag
     3301 acattttaaa tattttttgt cgacaaaact gataatcgca ccgacaccaa aaaagtagaa
     3361 ggatttggaa aaaaaagccc catcgggacg aaaaaagaac tcgctagtca atgataagtg
     3421 ttcataaatt aaattaaaat ttaacaaacc gaaattcaac aaaattatgt atgaaatata
     3481 taacagtgat ccatataatg caaatgcatt aactatttta ataaaaatct cacatgaaaa
     3541 tacttttaaa gtatatctta gtgtaaatag atatataagt gacagcataa aaaggtatgg
     3601 ttgcacatca aacagtatat catttatttt attaccagct aataagccga caaagaagcc
     3661 ataaaaagga aataaaagca accatataaa aagactgaca catttttggg tgattgaaaa
     3721 attgaatgta actaaattaa aaattaaaag tataaaacta aatacaaata ataaatatcg
     3781 caaagggaat attcccaatg gctccagtaa tcttccacca ccaccaataa tcagttcaat
     3841 acataaaaat gttataaaaa ttttatttat attattcata ttcgtaaggt gatgtttttt
     3901 aaaatatgtt taaaatcaac attaataaaa tataaatttt atcagccttt ttccgcctta
     3961 acttccaaaa acttaaagcc aagatcaaat tgcctatata acctttaccc aattgattat
     4021 tcacaaagct atcatcagct aaggcattag ctattttagt tacttctttt cgataccact
     4081 tgtttctcac caaaccaagc cgtttgtatt ttgctttaaa actaatgttt gctcccactt
     4141 ggttgttttc atgctgtctg tacagcattg ttggttttct atcaatatac cacgaataat
     4201 ttctcgtccg cgcaaatgaa tataaaaacc aatcatgcag acagatatct tgtgcagcat
     4261 ttttattatt tatgatgaac ttcttaaatt caataagcgt ttcttttttt ataacatatg
     4321 tacaccccgg acctgctgct tcaaaaaaat aatcgaattg agtttgcgac tgagccttat
     4381 caactaaatt ttttcgccct gatggataat atgcaattac attgctggaa taacaattcg
     4441 cattattaaa taccaagcag tcaattgcat tttttatttt atctttatac caaatatcgt
     4501 cctgatcact aaaacagata taatcatagt tttcaagatc gacatcttta attaatctat
     4561 aaaaattttt acctgccccc ccaaacttat tgacgcttgg aagtatattt ataattgagg
     4621 gatatgataa ttgaaattct tcacaaatat ttaatgtatt atcagttgat aaatcatctg
     4681 atataaaaat tgagatgtct acatcttttt gttcaataat actttttaat tgttcctcaa
     4741 tccagcattc gccattatac gtagccataa gtacagctac tcttttttga tcagaactat
     4801 gcattataac gaccgccccc agtaattcct cttattccat ccagcgtagc caaaatatgt
     4861 tttttcatat attcaatttt atttttttga gttgcgataa ttaaagtcgt atgaattaaa
     4921 gaaaaaacaa cttccctaat agagagtaaa agaggcacat gattttttct aagcaaaaga
     4981 aatgcatttc ttactcgata ataacgacgt actggcgagt gaattggtat gttaatccca
     5041 cagattttta ctgacttatc cccaatagca tgaaccattt ctatatcagg aatgatatga
     5101 actaatatgc catagtttaa gcatctaaga caccattctg tatcgacata atcaataaaa
     5161 agttcctcat ccatcattcc aacaatctca aaaacttctt ttgaaaccat ggttccggat
     5221 gagattgtta ctgaactttt aaacggtgtc tgtccttcag tgacatgaat tttctctcga
     5281 agaccatttt ttttgatatt acaaatagga taataatgtg acttatctct atcaataaaa
     5341 actgggccag ctatttttgc attttcatta tccatatagc aggaaagttt tttgataaac
     5401 tcattgcgaa tacggctatc ttgatcaaaa aaaattatct tctctgcccc tttttcaaga
     5461 gaatatcgaa tcccaatatt ttgtgctttt gctatacctt cattcctacc aagacaaatt
     5521 aaatgaactt tttcaatgtc tgctaattta tctttcaagg tgaaattagt cctattatcg
     5581 actactacaa cgtaacaatt ttgctccttt agttcagtga tcagtgcatt cagattttta
     5641 agttcgggat tatatgtcac aatgacagcg taaatattac tattcattat ccaagtgact
     5701 cagtaattgg ttaattttag ttttcatata cagaacacat tctagtataa agccattgta
     5761 ataacagagc atctaaaatt acacgtatgc tccaggatat tgcggcaccc agtaacccat
     5821 atttttcgac cataaaatac aaaagggcta aataaggaac caattcagca caatgaatca
     5881 aagcagtaat tttagatttt cctaatgcct ggattgcaga gaatggtatc attgccagcg
     5941 cattgaagaa aaaaccgatc aataacacat ttaatatatt aaaggaatta atttgataat
     6001 tctcaccaaa ccatattttc aatacaagcc ctgaatataa caagcctatg attatcaccg
     6061 gcaaacagat tagaaacatt aaaagcaatg atttatttac attacgttta aattctttaa
     6121 agtctttgat gttacttaac cttggaaaca ctgccctccc gattgctgca ggtattattc
     6181 ctaattttag aataacctcc gctggtgcag aataaaatgc aactttatct gcccccataa
     6241 tatttgacac tataaatcga tcaaaataca ccatgaccgg acttatgata ttacttactg
     6301 tcatccaacc gccaaagaaa aagagtcgtt taaaggtttt ataacaaaaa tgaacccccg
     6361 aaattttaat atcattcctg acataatagg cagagactaa aatcgaaata actcttgcaa
     6421 atattaaacc agcaaccgct gccgacaatg tagcactata aaaaacaaat atggccggaa
     6481 ttcccgcgat gcaagagctt gatatagatt tttgaatatt tacaatgcca aatttttcat
     6541 ccccctcaag aatggctgac cataattgat ttagaataaa taatggaatg caaatagcca
     6601 acagtttaaa tgctagttgt atatcactat gttcaacacc agaaattttt agataattaa
     6661 caattccatc agagaaaatc aataataaaa aagcaccaaa gcatgaaaat agcaccaaaa
     6721 aagatgtact tgttgaaatt accttggttc tttcatgatg attatcacga tgaattgcaa
     6781 tttctctaat aacagagcga gtcaggccta catcaaaaat tccagcatac ccaacaagtg
     6841 caattgcaat tgtataaaca ccgaaacctt caggccccaa acctcgagcg agaaatccta
     6901 gcgaggggat agcaattaaa gttggtattg catagccaaa aaggttccag acactatttt
     6961 ttattatgct cattattaca tttctttatc atataaaata atcagcatct acaaattttt
     7021 gaccattaat atctttatca gaaagcaatg gattttgaac cggccattct atatttatac
     7081 ttttgtcgct ccataaaatc ccacgctctt gcatttttga atagaaatta tttgttttgt
     7141 agacaaattc tgcttcatca ctaagaacgc aaaacccatg agcgaatcct tccggtatcc
     7201 ataattgacg cttattctcc gccgacaaat ttacaccaac ccacttgcca aatgttggtg
     7261 atgatttccg aatatcaaca gctacgtcaa aaacttcacc gacaacacag cgtaccagtt
     7321 ttccttgagc ataaggttcc agctgatagt gcagtccgcg taacacaccc ttagttgatt
     7381 tagaatggtt atcctgaaca aactcaacct ttcgtcctac agcctcttcg aaaactttct
     7441 gattgaagct ttccataaaa aagccccgct catcaccaaa tacttttggt tcaaaaatta
     7501 gcacatcagg aatttcagtt ttaattacgt tcattttatt aataaccttt aatcattttc
     7561 agcagatact gaccataagc atttttcttc agcggctcgg ctaatacctt cacttgctca
     7621 gcatcaataa agcctttacg gtgagcaatt tcttccgggc aggaaacttt cagcccctga
     7681 cgctcttcaa ttgttgcaat gaagttgctt gcttcaataa gactttgatg tgtccccgtg
     7741 tccagccatg cataaccacg ccccatcata gcgacagaca aacgtccttg gtccatataa
     7801 atacggttaa tatcggtaat ttccagttcg ccacgtgcag atggtttaag gtttttcgcc
     7861 atttctacaa cgtcattgtc atagaaataa agcccagtaa ccgcatagtt actttttggt
     7921 tcctgcggtt tttcttccag actaattgcc gtaccgttat catcaaattc caccacaccg
     7981 tagcgctcag gatcatttac gtgataggca aaaaccgttg cgccactctc tctgttaaca
     8041 gcggtatcca ttaatttcgg caggtcgtga ccatagaaga tattatcacc aagtaccagc
     8101 gcacaatcat caccacctat aaattcttca ccaatgataa atgcttgcgc cagaccatcc
     8161 ggactcggtt gtactttata ttgcagatta agcccccact ggcttccatc accaagcaat
     8221 tgttgaaaac gtggtgtatc ctgcggcgta ctgataatca gaatatctcg aatacccgcc
     8281 aacataagcg ttgaaagcgg ataataaatc atcggtttat cataaatcgg tagcagttgt
     8341 ttgctcacag ccattgtcac aggataaaga cgagtgccgg aaccaccagc cagaataata
     8401 cctttacgcg ttttcatttc accattcctt ttaattcatc tcgctctagc atcatgagcg
     8461 agatgcaata atttgttaaa ttgccgtggt cgtaaataat tcgttgagca tacgtttcac
     8521 gcccacctgc cagtcaggca agacaagcgc aaagttctgc tgaaattttt cggtattgag
     8581 gcgagaatta tggggacgac gtgctggtgt gggataggcc gttgtcggca cggcattaag
     8641 tttgttaaga gcaaggttga tccccgctct gcgcgcttct tcaaatacca gagcggcata
     8701 atcgtgccag gttgttgtac caccagcaac cagatggtac aaaccagcaa cttctggttt
     8761 atttgcggcc acgcgaatgg catgagcggt gcaatcagcc agtaattcag cccccgttgg
     8821 tgcgccgaat tgatcgttta ttacagccaa ctcttcgcgt tcttttgcca ggcgcaacat
     8881 cgttttggca aagttatttc ctttaccagc gtatacccag ctggtacgga aaataagatg
     8941 ctttgcgcaa tgttcttgta acgctttttc cccagctagc ttggtttcac cgtagacatt
     9001 tagcggtgct gttgcatccg tttccagcca tggcgtgtca ccgttgcctg ggaaaacata
     9061 atcagtggag tagtgtataa cccaggctcc gacttcatta gctgcttttg caatcgcttc
     9121 gacgcatgtc gcgttaagta attgcgcgaa attcggttct gattccgctt tgtctactgc
     9181 agtgtgagca gcagcattaa caataacatc ggggcgaatt tttttgacgg tttcagccac
     9241 accttcgggg ttgctgaaat ctccacaata atcagtggag tgaacatcga gcgcaatcaa
     9301 gttacccaga ggagccagag cacgctgcag ttcccaaccc acctgccctg ttttgccgaa
     9361 aagcaggata ttcattactg acggccctca tagttttgtt caatccacga ctgataagta
     9421 ccgcttttca cattctcaac ccaatttgta ttagccagat accattccac cgttttacga
     9481 atcccgctct caaacgtttc ttgcggtttc cagcccaatt cgcggctaat tttatcggca
     9541 tcaatggcgt aacggcggtc atgccccggg cgatcggcaa cataagtaat ttgctcacga
     9601 taagatttct ctttcggtac aatctcatcc aacaaatcac aaatagtgag cactacgtcg
     9661 atgtttttct tttcgttatg tccaccaatg ttataagttt cacccgcttt accttcggtt
     9721 acgacggtat ataacgcacg agcatgatcc tctacataca accagtcgcg aatttgatcc
     9781 cctttaccat aaataggtaa tgccttacct tccagagcat tcagaataac caatggaata
     9841 agtttttccg ggaagtgata aggaccataa ttgttcgagc aattagtcac aatggttggt
     9901 aaaccataag tccgcttcca ggcacgtacc aaatgatcgc tggaagcttt agacgcagaa
     9961 tacgggctac ttggcgcgta agctgtcgtt tccgtaaata gcggtaacgc ttcgttatta
    10021 tttgcttcat ccggatgggg taagtcacca taaacttcat cagtagaaat atgatgaaag
    10081 cggaaacttt ttttcttttc atcattcaga gcagaccagt aattgcgcgc cgcttctaaa
    10141 agaacataag tccccacaat attggtttca ataaatgccg cagggcccgt tattgaacgg
    10201 tcaacgtggc tctctgccgc caggtgcatc accgcgtctg gctggtgctg tgcgaaaata
    10261 cgcgacatgg ctacagcatc gcagatatct gcatgctcaa aagcataacg ttcagaatca
    10321 gaaacatcag cgagtgattc caggttcccg gcgtatgtta atttatcgac attaacaaca
    10381 ctatcttgcg tattatttat tatgtgacga acaacagcag aaccaataaa ccctgcgcca
    10441 ccagtaacaa gtatcttcac ttttttattc catatagcta tagagcatgc tgtgaaatag
    10501 actgctccag atttgattaa tagaggtatt aatacacgct accgcccctg gcttaacagc
    10561 taccagtgca ctgcatgcat ctcaacgatg tggcgagcgt tagccactcg cgccaaatcc
    10621 gaaaaattca aacgttaatt gtcttaccaa acagccgtag aaacaaggaa aatcctggaa
    10681 aaatttgaat aatatgagct atgtaacttc ttgtttttat aggctttata aaaacggcaa
    10741 cggcaggaaa attcaatgag caaaacttat gccacgaatc ttcactgccg ttataatttt
    10801 cttatcaacc gttacatccg gtcagatttt cattattcgc ttaacagctt ctcaatacct
    10861 ttacggaact tcgccccttc tttcaggttg cgtaggccat acttcacaaa cgcctgcata
    10921 tagcccattt ttttgccgca gtcgtaactg tcgccggtca tcagcattgc atcaacggat
    10981 tgttttttcg ccagctcggc aatagcatca gtcagctgaa tacgtcccca tgcaccaggc
    11041 tgagtacgtt ccagttccgg ccaaatatcg gcagaaagca catagcgacc tacggccatg
    11101 atgtctgagt ccagcgtctg cggctgatcc ggtttttcga taaattcaac aatgcggctg
    11161 actttaccct cacggtccag cggctcttta gtctggatga cggagtattc agagaggtca
    11221 cccggcatac gttttgccag cacctggctg cggcccgttt cgttgaaacg tgcaatcatg
    11281 gcagcaaggt tgtaacgtag cgggtcggcg ctggcatcgt cgatcacaac gtctggcagt
    11341 accacgacaa atgggttgtc accaatggca ggtcgcgcac acaaaatgga gtggcctaaa
    11401 cctaaaggtt cgccctgacg cacgttcata atgatcacgc ccggcggaca gatggactgt
    11461 acttccgcca gcagttgacg cttcacgcgc tgctcaagga gtgattctaa ctcataagag
    11521 gtgtcgaagt ggttttcgac cgcgttcttg gacgcgtgag ttaccaggag gatttctttg
    11581 atccctgcag ccacaatctc gtcaacaatg tactgaatca ttggcttgtc gacgattggt
    11641 agcatctctt tgggtatcgc cttagtggca ggcaacatat gcatcccgag acccgctaca
    11701 ggaataactg cttttaaatt cgtcattatt tcatccacct gtaaaatggt tgctgaatta
    11761 tagcttgttc gatttttttc gccagcatca attaccctga attgattact gaattacttg
    11821 tgatgttacg ccgcttcgtt gtggattgca gtagcattgt tcctaagtat ggctccattt
    11881 ttccaggaat ggtcgcaaat ctactccctc cgttccggca atctaaagtt aatcttttcc
    11941 acattaacaa tatggtgatt aatcctgtcg atatcgacgg agctttgccc tctttcattc
    12001 accgcatgaa catttgcaag agacagcagt gtttcttttt tcgccataaa gacgccgcga
    12061 acgtctttgc gcatgtcgaa gttcatgctc aatgctggtc caactgagga ttcctgcatc
    12121 acattgatat ttcgcataaa aagatgttgc ggcttgttgt gtaactccag cgaggcacgc
    12181 ttcatctcaa tgttagttag cgacacaaag gagacggcat tcccggcgga gatttggatg
    12241 ccgcgcaatt tataagcaag atgggtatta tccagttgaa tattattcac tcggaaattt
    12301 tgcggtatcg agagatattt gcctttaatt accccatagc cgattaacat cccggcgcta
    12361 ttaatcattt caatattatc aatcacgaaa ttgtcacaac cgtaaatagc gactgtcgcg
    12421 ttatcaatgc ccgctttctt actgaaatcc ggcgtgatat tgcgggcatt gatattacga
    12481 ataacaaaat gtttaccatt ttcaacatgg atcaactgcc gacaatccga tcccgtgata
    12541 ttcgccacga caaagttttt cactgcctgg tcttccgggt agttgttatc ataagtgctt
    12601 cccgcaaggc ctatgccgat gccccagttg attttgccgt tggtacagtt gatacgctcg
    12661 atgacatggt cagatatcaa aatatcactg tcgttaattg ccacgttcca ttcaatggcg
    12721 tcgccctgta agtcgctgaa cttacagttg gtaatgttgg caccgataat ctggttatga
    12781 aatccctggc gtaagatggc gtaattagcg tggctgacag tgaggttatc gatggtcagg
    12841 ttgcgcatga cccgtttgtt tttgccgcca atataaatct gcattaccgg gccaaagccg
    12901 ctcatcgcca gccctttgat ggtgcagtca gaaccacgca catccagggt aatgttatgc
    12961 ataccgccgc cctctccccc tgtcacctgg ctgccgtcct gtaagacaaa tcgccctctg
    13021 ccgttgccgc gcaggcttcc aaggatgtgt aacgttttgc cgggagggat gaagatgccg
    13081 gtgttgatat tgtcacaaac gaatccggca ggcacgacga ccgtttgccc ttcgctgaag
    13141 gcttgtttaa atgaggcgat ccagtcatgt gggttgtagt cgttaatgtt aacgctttgt
    13201 cgggcgggaa gtgcgcgagc gaaaggggta tggaggaagg caagcgccga gcttgccgtc
    13261 aggaaggtgc gtcgggagag tgttttaaat ggcatacgtt ctcctctata aagcctgcag
    13321 caagctggcg agttctcgat taatcacctg ctggttaaaa tcgtgttcga ctttttcgca
    13381 cgcacgtttg acgaccggag ccagttcgtc ggtatccagt tggctaaacg ccgccagacg
    13441 ttgtgccagt gcgcgagcat cgttctcagg cagcagccag ccggatttat cggcctccac
    13501 cagttcagga attccgctat gcagagtaga aaccaccgga ataccgaccg ccatcgcttc
    13561 cattagcgcc accggaatgc cttccatatc gccatccgcg cccgttaccg atggcaacag
    13621 gaagacatcc gcgtcgtcga gcatctcctt cacttcatgg ctcggtttaa agcccggcat
    13681 ctctaccaca tcttccagtt gatattgttc gatgatggtg cgcaggcgtc gttcccacag
    13741 gccaatgccg aggatgcgat agcgaaatgc cacgccctgc tctttcaact gacggcaggc
    13801 ttcaatcgcc acatgcaggc cttttttctc ggttaagcgt gcgacagata tgatttccag
    13861 cggcgttgcg ggcgctttca ccggacgcgg gctaaagcgc gtcatgtcca cgcccatacg
    13921 cgatacggcg attttttctc tggggcagcc cattttttgc agccttccgg cccacagatc
    13981 gcttatcggt aacatcaggt cgccacggcg aaacagttgt tgatattcgg gagtgtagtg
    14041 gttgagcact tcccgactgg agatatcaat accgtggaag atggtggcaa ttttgccgcg
    14101 aatgataccc agttcgcgta gttttgctgc ggttaccccc gccgggccaa aatgagcgat
    14161 gaacacatcg gcgcgaaacg gtgttgcgac ctgaccacaa atggcagaca aaatcaggtt
    14221 ccgcgactcg gcaccatagc gtttgaggtt gagcgcatgc caggtatttt tacgatgaat
    14281 gccacgtaag gtctggctgg cgcggtggcg cagtttcgct actttgcccg caggttcgtc
    14341 ctgtaaccag cgggttctgg cgacaaggtt gtatttcgtc catgccgcgt gggtgttctc
    14401 tgtatcgcct ttttgcaaag cgacaatctc cacttcaaag cccatatcaa taaacgcggt
    14461 aatttgattg aggacgaagg tttccgacga cagcgggaat ttcaataaga agaagccgac
    14521 cttcatttca cctccccgat gcgctcaagc acagactgca ccatctgcat tcccgtctga
    14581 cgttcgcgac tgacggcttc gttaaatcga gcgttcagca ccggaagctg gcctaaggta
    14641 tccgcaacca tcgcttgcag gctgccgtcc agtaaatgac gaatatcaat cgccatctct
    14701 ggcagtccca gctgctgcat aatcccggcg gatttatgtt catagttgat ggcaatcgcc
    14761 ggagtagcaa agttcattga gataatggcg gagtgcaggc gcgtaccgac ggtgagttca
    14821 caggctccga gaattttgcc catttccaga tcgttgagtt catccatcac tacgtggtaa
    14881 cgggcaggat cgctgatgtg ctggcgcaga ttgagcgcca ccatgcggtc gtctttgtta
    14941 tagctgtcga tacccgtaca ggtggagagc gcaatcacct gatagccttc atcgagaatg
    15001 cgattgacca ccccggcaaa ggctttttca tacgcttgtt gagtggtgcc gagacgtttg
    15061 tcgaacggtg ccagttcgcg cagggtaatt gccactgttt tctgttgtgc ggcaacgtcc
    15121 agccagtgtt gaacggcata gctggcggtg aagtcttcgg tgtggtgatc gaccagccac
    15181 gcggtatcga cgccatgttc cacttttgcg gtggtgatat ttctgcgttt catcaaatca
    15241 aggctgaccg attcgcgcag gatcagcgcg tcgcagtgac caaagacata gttcgccagt
    15301 tggttaaatt gctcatcctg gaaggggccg acgctgtgac caatcataaa cagcggcttt
    15361 ttcgccataa atgtgcaaag cgcatgttca aactgcggca cgccgtagag atcgacaaaa
    15421 aacgatccgc cgacctgaat aatggcgtcg taacctgaca gcaggcgcac gaagtcggtg
    15481 aatccctggg cgatggcgat attgcgcagc ttgccagtgt cggtgacgcg tgagagcaat
    15541 acctgatgct ggtagcggcg acggaggact tttttaatgc gcccgacaac gcccgccgcg
    15601 ctattgtgtt gtgtcatctg caggaacagc ggatcgccca ttaccgggcg gttgagcagc
    15661 caggaagaac tgaccggata gcggctcatc acgtccactt cggtatgtgg attgaggatg
    15721 ttgatggcat caagtaagcc gcgcaagatg gcgctgtcgc cacgattgcc gcaagtgtgg
    15781 ttgcccagaa taagtaattt cattttttcc tcataaattt gatgccaggt gaggctgtgt
    15841 ttgttgtgtt cgttgccgtt ttttgcctga tgcgacgctg gcgcgtctta tcaggcctac
    15901 aggtcccgag cacagaaccg taggacggat aaggcgtttt acgccgcatc cggcaaccgt
    15961 tgtcggaacc gaaaacagca attcaccccg cccgtaaaag cattttcatt ttttcgctgc
    16021 gacaaaactg acgcttcact tccaccacca gcggatggcg tgacagcaca atcatcacca
    16081 caaacgccag cacgcccgcg gctatttgca ccgccagcag catccccagc gccagttgcc
    16141 ctttcagcac aatgcccagc gcataactga ccaccagcgt cggcaacgag agataaaacg
    16201 gcagccacaa actcaggatg tactggcgat aactggaacc aagaaccggt ttaatcatca
    16261 cgaagtaact cagaatggtg ttgataatct gcaccagcag gaaaccgagc gtgacgccga
    16321 ttgcgcccgc catctgccca ccaataacaa tcgccggaat aaacagaaat gttttgaata
    16381 cgttgaattt aaagctgata tcgacccgcg ctttcgccat cagcagcgaa ccaatcgggt
    16441 tacccacgga gcgcagcaga cccacaatac acagcaattg cagcaccgga ataatgctgt
    16501 tccacttttc gccgaatacc agcggtacga agttattcga caccaccatc agccccagca
    16561 gcgccggaaa gttgataatc cccactatcg acagcagctt gtagaagtta acacgcagct
    16621 tttcggtgtc gtcctgaatt ttggcaaacg ccggaaacaa cacgcgggtg atgattgggt
    16681 tcagcttcat cggcggtacg acggccacgt tgtacgccag gttgtatccc cctgccacgc
    16741 ccgcgccaag aatacgcgcc agcaccagcg ttgaaaggtt ggtgttgaga tagttgatga
    16801 tgctgtccgc cgtcagccag gcaccaaaac gtaagttcgg tgccacagac gccagcgaga
    16861 agtgcagacc ggggcaataa attttgcgtc caaagtagcc aaacagcagt gttctcaccg
    16921 cactattgac cagataaccg aggatcgcgg tcatcgccag cggccagaaa tgggcgctaa
    16981 ctaccgtaaa agtgaagccc gccagcaccg cgctggtttc gatcatgccg attttgttga
    17041 actccagctc cttctgcatc aacgcgcgga actgttgccc gtgggggatc accacaaacg
    17101 ccagcgacaa tgttttaatc aacggtgcca ggtccgggtt attcagcacg tcgccgatga
    17161 catcactcaa caaaaacacc gccacacata ccacaagccc cagcccgacg ttcagccagt
    17221 acagcgtggt gagttcaagg tgactgattt cttttcgctg aataatcgag ttagcgatac
    17281 cgaagtcaga aagcgtatct gccagcgcga taatcaccag cgacacagtc agcaggccga
    17341 actggtggtt gtcgataatc cgcgccagca cggtcatctg caccagcccg aggccgatga
    17401 tgatcaccgt ggcaatagcc gaccacttcg cgccgctgat ggttttttca cgtaagctca
    17461 tatcaatatg ccgctttgtt aacgaagccc ttgaataccg tcaggaaaac gattttgata
    17521 tcgaaccaga cgctccattc gcggatgtac tcaaggtcga actcgacgcg tttttccatt
    17581 ttctccagcg tgtcggtttc gccgcgccag ccattaatct gcgcccagcc ggtaatgccc
    17641 ggtttcactt tatggcgcag catgtagcct tcaatgagct ggcgatactg ttcgttatgc
    17701 gctactgcgt gcggacgtgg accgacaatc gacatccccc cggtcagcac attgataaac
    17761 tgcggcaatt cgtccagcga ggtgcggcgc agaaagttcc ccactttagt gacgcgcgga
    17821 tcgttctgcg tcgcctgggt caccactttg tcgttctcca tcactttcat ggaacggaac
    17881 ttccacactt tgatcggctt gccatccata ccatagcgag tctggcggaa aataaccggc
    17941 cctggtgaac tgagtttcac cgccagcgca atacagcaca gcaccgggga gatcagcagc
    18001 agaataagcg tcgccagcac aatgtcttcc gcacgtttta gcaggcggtt aacccccgaa
    18061 agcggcgtgt catacagcgg caccaccgga acgccgttca tctcttcgag acgtgaatgg
    18121 agaatgttga aggtaaagac gtcggggatc agcagcaccg aacaggtggt atccgccagt
    18181 tgatggacca gttttttcac tcgtgcgccg tcgcacattt gcatcgcgat atagacgtta
    18241 tgaatcttgc ccgcttttgc atcctcgacc agctgttgca ggttacccgc ccagtcgtta
    18301 gaaacgccgc ccggtttcgg gtcgtggtaa acgcccacca cttcaaaccc taaccacggc
    18361 tgattacgga aactctccat cagcatttgt ccggcggcta aatcccccgc tacggcgacc
    18421 atccgcttgt tgtagccatg attacgcagc cagcccgccc caatgcgaat acatgaacgg
    18481 caaaccacca gtccgatgct ggtcagccca taccacgcca gccagatttt cagttgcgtg
    18541 tcgaaatcat tgttgaacgc caccagtccg gcgctgaaaa tcacgcttaa ggtccagttt
    18601 tgtagcaaca gggcaaattc tgtcgctgcc cgaacaccgc gccatgagcg ataaaaatcg
    18661 gtgatgccgc ccagcatctg gaacaccacc agcgtaatca gcgccaccaa caggtgcatg
    18721 tagaggaatg acagtccgct gacttcgcaa accagccata gcccggcaaa catgatggtg
    18781 atatctgaaa agcgttgcac catagagatt aacgatgcat tggttttcgc tcgctcgcgc
    18841 ttttttagat ttgtcatcgt tgttcctgtt attagcccct tacccgcagt ggggtaaggg
    18901 aagatccgac attactcgtt cagcaacgtc agcagagttc gcgttcgcgc ttccatcagc
    18961 ggcacatcac cgcgcgattc cacattcagg cgcaccaccg gttcggtatt ggaggtgcgc
    19021 aggttaaagc gccagtcggc aaaggtcatg ctgatgccat cgctgcgatc caccgccagc
    19081 gcctcacggc taaaatgctg ttccacgcgg ttaatcgcct caacgggttg cgccagtttg
    19141 ctgttgatct caccgcttgc cggaaacgcc gccatccggt cgcgcaccag ttcgcccagc
    19201 gtttttcctt tcaggcacac cagttcggcg accagcagcc acgggatcat gccgctgtcg
    19261 cagtaagcga aatcacggaa gtaatggtgg gcgctcattt cgccaccgta gatggcgtct
    19321 tccttgcgca tccgttcttt aataaaggcg tgtccggttt tcgacattac cggcgtgccg
    19381 cctgcagcag tcaccacatc aatagtgttc caagagagac gcggatcatg gatgatcttc
    19441 gcgccgggat ttttttcgag gaacgcttct gccagcaggc cgacaatgta gtagccctcg
    19501 ataaactgcc ctttttcgtc aaacagaaaa cagcggtcaa aatcgccatc aaaggcaatg
    19561 cccatatccg cgccgtgttt gatgaccgca ttgcgggtgt cgtcgcgaca ttccggcagc
    19621 agcgggttag gaataccgtt ggggaaattg ccgtccggtg tgttgtgtac tttgattaat
    19681 tccacgggcg cgccgagggc tttaaagcgg gcttcaatgg cgtccaccac cggacccgct
    19741 gcgccattcc cggagttgat caccagcttg agcggcgtga gatttttgac attgatataa
    19801 ccgaacaggt gatcaacgta agcgtcacgc aggttgattt gctgatagcg accgcgtttg
    19861 gtttcatcga cgggaggaaa gtcgttggct tcagccagac gctggacgtc gcgcagtccg
    19921 gtatcaccgc tgatcgtgcg agccccctcg cgcaccagct tcatgccgtt gtaatccatc
    19981 ggattatggc tggcagtaac ttcaatgccg ccatccacgc cgagatggaa cgtggcgaaa
    20041 tagatctctt cggtgccgga cataccgata tccagcacat cgacgcccgc atcctgtaaa
    20101 ccttttgcca gcgccagttt taaggtttcg ctggtgagac ggacatcacc gcctaacaca
    20161 atggttttcg gtttgagaaa ttcgccatag gcgcgaccaa tgcgccaggc gatatcttca
    20221 ttcagttctt cgcctaattt cccgcgaata tcataggctt taaagcaggt taattttttc
    20281 atgtcgttac cctttttcag gcaatagttg gccctgaccg aaacggccag ttttattttt
    20341 gtcgttattt acaccgcggt ttcgcattca ttgcctgatg cgacgctgcc gcgtcttatc
    20401 aggcctacaa aaccgaaccg taggtcggat aaggcgttca cgccgcatcc gacaaacagc
    20461 gcctgatgcg acgtttacac ccgtccgtag cgatccgcga agcgcaccac atcatcctct
    20521 tcgagataag agccggagcg cacttcaatt aaatcgagcg gaattttccc cgggttttcc
    20581 aggcagtgcg tcgcccccag cggaatataa atggactcgt tttcaccaag cagtttgata
    20641 tcaccatcaa tggtgacttt tgccgttccc gcgaccacta cccagtgttc cgcgcggtga
    20701 tggtgcatct gtaccgacaa accctcgccc ggtttcacgg tgatgcgttt cacctggtag
    20761 cggtcgcccg cgtcgataga gtcatatttg ccccacggac gatacacttc gcgatgcacc
    20821 cgatgctcat ggcgaccatc ggctttgatc tgctcgacca cttttttcac atcctgtacc
    20881 gcgttacggt cggcaatcag caccgcatct ttggtctgca ccactaccag atctttcacc
    20941 ccgacggtgg tgaccaggcc tgattcggca tacacatagc tgttttcagt tttgtgatta
    21001 atcacatcgc cgtggcaaac gttgccctcg gcggtgtggg cgctgatctc ccataatgaa
    21061 gaccaggaac caacatcgct ccagcccgca tccatcggca ccacaacagc atctgccgta
    21121 ggttccatga ccgcgtaatc caccgactct tccgggcagg cgagaaacgc ttcttcatcc
    21181 acacgaataa aatcgagatc cggatcgacg gcgctcatcg ctttttcgca ggcatcgagg
    21241 atatccgggc gatatttttt cagttcttcg agatagcgtc cggcgcggaa caggaacatg
    21301 ccgctgttcc agtaatattc gccgcttgct acataggcct gggcggtttc cagattcggt
    21361 ttttcgacaa actgcgccac ttcaaaggcc accgtatcct gctcacccac agacacttca
    21421 ccgcgacgaa tatagccata accggtttct ggtagatccg gcacaatgcc aaaggtcacc
    21481 agcttgcccg cttcggcata aggcatggca ttacgcacgg cggcacggaa cgcgtcttca
    21541 tcggcaatca catgatccgc cgccaatacc agcattaacg ggtcgctctc cgggctatga
    21601 cgttttgccg ccagcgccgc cagtgcaatg gcaggtgcag tgttacgccc tgccggttcg
    21661 agaataatgt tctcggtgag tttgttcagt tgacgcagct gttccgcgac aataaagcgg
    21721 tgctgctcat tgcaaatcac caccgggctt tcgcactcca cgccgttcag gcggcagatg
    21781 gtggtttgca gcatggtgag atcgcctttc aggcataaaa actgcttggg gtaaagtacg
    21841 cgggaaagcg gccataaacg gctaccggag ccacctgcca tcacaactgg atagagtttc
    21901 gactgcgcca taattatccc cgaatatcat ttataaattg acgtaacacg ttctctttat
    21961 cgagcgtgcg ttcggcatat tcacgtgcca ccgtgttatg tttgggcagc aggagcgcct
    22021 gacgaatccc cgccaccagc gcctcgaccg attccggttc tacgcaaacc gcaatacccg
    22081 gaaaggtttc gcaaagctgc cccagttctg tgtgggcctc tgcggtaatc accgcgttac
    22141 cgcctaccgc cagaatattg gtcagtttcg acggcaatac ggcatctgcc gcgccgcgtt
    22201 tttgcaccac cagatggcaa tcgcccatct tcagcagtgc aggtaaagcg tcatacgatt
    22261 gcagcgggaa aaattgcatg ttgcgcagtc cacgctgctg cgccattttt tccagccgcg
    22321 ctttgccgcc gccctgcccg acaatggcaa aaatcagcgg ttcatcgcgc aggagatcgg
    22381 cagcttcaat aacgttttcc agcccctgct tttcaccaat attgccggag taaagaatga
    22441 tttttttgtt atccggcagg ccaagctggt tacgaagggc atcaacatcg gcgtctgcaa
    22501 catgctgaaa acgggcaatt tccgaccagt tggggaagaa gatgacgtta tccgccgcca
    22561 cgcctttttc gatggcttta ttcatcatcg aacgcgaaat cgtggaaacg ttatcgacgt
    22621 tatgcagtcc gctacgttcg aacgccgttg ccagctgtgc cactttgccg cctttgcctt
    22681 ttccggcaag gcccagcccc agcatggcgt ccacttcgta atcctgaata tgcagcacgg
    22741 tacgcgcacc agagagcttc gccagcaggc gcattcccgg cgtgcaaaac agcgttggca
    22801 ctacgccaat aatgcgatcc ggcttccagc gacgttgcgc catcagtgga aagaaactgc
    22861 tgacggcaaa actgccgaga tgcaacaagc gtttcagggt gctcggctgt tttggcacgt
    22921 acagcgggca gcgccacacc gtggcggccc cttcttctcg tttgtagcgc caggcggaat
    22981 agttctcacc cacctgccat tgcgggtagt aaggcggtgc ggtaatgacc cgcacctcat
    23041 gaccttgtgc cgccaaccat tccaccatct cgccggtgta tttgccgatg ccggttaact
    23101 ccggcgagta gttaatgccg tagaccagta ttttcataat ccgggtactc cggcacgctt
    23161 ctcagcgaga aaataggctc ggctgttagc gtgaacatcg ttactggcga gcagcgcgtc
    23221 cggcgtcagc cagcggtaat cgtcatgctg ctcatccggc agtaacagct cttcttccgc
    23281 tactctgaag cgaaaaccga gcaccacata gtgagtggtg aaatccgtgc cggagaagtt
    23341 atcgtcataa aaatgctgcc agacaccgta aaactggcct gctgttatcg gcagacgcag
    23401 ccccagttcc gccatcgtca gccgctcaaa tgcggcttcc agcgtttcgt ctttctgcac
    23461 gcgccctccc ggcacaaacc agtaaccctg cgccgggcgg ttggttcttt tgccaagcag
    23521 aaactcgccg cgactgttct cgacaataaa gtcgagagag acaagcggtg tggagcgcac
    23581 tacagtggca aagtcttcct gacgtaaaaa catcattccc cccgaaagcg gtcttgattc
    23641 tcaaggaacc actggtaagt gctggcaagc cccgcttcca gtgagatttc gtgataccag
    23701 ccaagctgat gcaggcgcgt cacatccagc agtttgcgcg gcgtaccatc cggtttgctg
    23761 gcatcaaaaa ccacccgacc tttgtaaccc accactttgg cgatggtttg cgccagctcg
    23821 cggatagtgc agtcaacgcc cgtgccgacg ttaatgtgcg acagcatcgg ttgggtattc
    23881 tccagccaga cttcatgcgc cagctccatg acatgaatgc tcgccgccgc catatcatcg
    23941 acgtgcagga attcacgcat cggtgtaccg ctgccccata ccaccacgtc cggcgcattc
    24001 tgtgccgtcg cctcgtggaa gcgacgcagc aatgctggta tcacatggga gttactcggg
    24061 tggaagttgt catgcggccc gtacaggttg gtcggcatga cagtgcggta atcgcgtcca
    24121 tactggcggt tgtaagattc gcacagtttg atcccggcga ttttggcaat ggcgtaaggc
    24181 tcgttggtcg gctccagcgt gccctgcaat aactcgcttt ctgccatcgg ctgttttgcc
    24241 agtttcgggt agatgcagga cgatccgaga aacatcagtt tgttcacgtc gttctgatgc
    24301 gcggcgtgaa tgatgttgct ctcaatcatc atgttctggt agatgaaatc cgccggatag
    24361 gtgttgttgg caacaatgcc gcccactttc gccgccgcca gatagacctg gtcaatgcgt
    24421 tcgctggcaa agaaatcatg cacggcgcgg ctgtccagca ggttcagttc atcgcgggta
    24481 cgtaatacca gctccacatc accgcgctgt tcgagctgcc gcttgatggc agaaccgacc
    24541 atcccacaat ggccagcgat aaaaatgcgt tgtttactca tgcttatgac tccagcgcga
    24601 tcgccacgtc gtagccgtga gatttcagca gagagtgttt tttcgccgct tcgaggtcat
    24661 tagccaccat ttcagacacc atctctctga gggtgatttc cggtttccag cccagttttt
    24721 cgtgcgcttt ggtcgggtcg ccgagcagcg tttcaacttc tgccggacgg aagtaacgcg
    24781 gatcaacggc gataatcaca tcacccggtt taacgcccgg cgcgtcatgc ccggtgacgg
    24841 aaaccacaat gcccttctct tcaacacccg tgccttcaaa gcgcagtttg atgcccagct
    24901 gtgctgccgc catttccaca aactgacgta cggagtactg aacgccggtc gcgataacga
    24961 aatcttccgg ctgttcctgt tgcagcatca tccactgcat ttttacgtag tctttggcgt
    25021 ggccccagtc acgcagggaa tccatattgc cgaggtacag gcacgactcc agcccctggg
    25081 cgatgttggc gattgcgcgg gtgattttgc gggtaacgaa ggtttcgccg cggcgcgggg
    25141 attcatggtt gaagagaatt ccgttacaag cgtacatgcc gtaggattcg cggtagttaa
    25201 cggtgatcca gtaggcgtac agtttggcga ccgcatacgg agatcgcggg tagaacggcg
    25261 tggtttcttt ctgcggaatt tcctgcacca gaccatacag ttcagaggtg gaagcctgat
    25321 agaaacgagt tttcttttcc agaccgagga agcggatcgc ctccagcagg cgcagcgtac
    25381 ccatcgcgtc gacgtcagcg gtatattctg gtgactcaaa agagaccgca acgtggctca
    25441 ttgcgcctag gttgtacact tcatccggct gtacttcacg caaaatgcgc gtcaggttag
    25501 aggtatcact caggtcgcca taatgcagat ggaatttcgg gttgcaggtg tgcggatcct
    25561 gataaatgtg atccacgcgc tcggtgttga atgacgatgc gcgacgctta ataccatgca
    25621 cctcgtaacc tttttccagc agaaactctg ccaggtaaga accgtcttgt ccggttacac
    25681 cggtgatgag agcgactttt gacatgtctt attcctctgt atttttgaat ttattcagtt
    25741 tcaacgcgtt cgcgtatcac cactgcggga ttcccccggc aaaccacatt tgccggaagc
    25801 gatttaaaaa cactgcttcg tgcacccacg acggtgccgt cgccgattgt gacgcctggg
    25861 gcaacaaaga catcggttgc cagccagcat ttctcgccaa tcacaatagg cgtggcgtta
    25921 atggtgaaat gttgacttgc atggtcgtgg ctaccggtgc ataaataact tttttgcgat
    25981 atcaccgaat gtgcgccaat ggttatttca ccgagggtat ataaattgac gtcatcgcca
    26041 acccacgcgt aatcacctaa ggttaatttc cacggatagg taatttttac tgacggacga
    26101 ataactacgt tttttcctat ttttgctccg aataaacgta ataaaaaagc ccgccagcga
    26161 tacaatactt gtggcgacca ggcaaatatt gttgcctgta ctgcccacca taattgcact
    26221 ttaatagcgt tgccgccccg gaaccctttc ggcaccgaga aaccgcttaa atcttgcatt
    26281 ggttttcctt atattcagac tttgttatat aaggctttag tcttacctgt cgtacgttgg
    26341 cgtaaatgcc aggataattc tgcccagaag ccaggtacat gtaatatctg ccgttggact
    26401 tttttggcgt cagcac
//
LOCUS       DAJTRD010000055        25848 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTRD010000055 DAJTRD010000000
VERSION     DAJTRD010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25848
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.055"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..199)
                     /locus_tag="OO315_002455"
     CDS             complement(<1..199)
                     /locus_tag="OO315_002455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5891334.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKTLA"
     gene            complement(332..637)
                     /locus_tag="OO315_002456"
                     /pseudo
     CDS             complement(332..637)
                     /locus_tag="OO315_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            705..1320
                     /gene="tnpA"
                     /locus_tag="OO315_002457"
                     /pseudo
     CDS             705..1320
                     /gene="tnpA"
                     /locus_tag="OO315_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1648..2730
                     /locus_tag="OO315_002458"
     CDS             1648..2730
                     /locus_tag="OO315_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5891335.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3219..4017)
                     /locus_tag="OO315_002459"
                     /pseudo
     CDS             complement(3219..4017)
                     /locus_tag="OO315_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4027..4578)
                     /gene="ybcL"
                     /locus_tag="OO315_002460"
     CDS             complement(4027..4578)
                     /gene="ybcL"
                     /locus_tag="OO315_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5891336.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4747..5079)
                     /gene="emrE"
                     /locus_tag="OO315_002461"
     CDS             complement(4747..5079)
                     /gene="emrE"
                     /locus_tag="OO315_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5891337.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5424..5738)
                     /gene="fliE"
                     /locus_tag="OO315_002462"
     CDS             complement(5424..5738)
                     /gene="fliE"
                     /locus_tag="OO315_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5891338.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5952..7611
                     /gene="fliF"
                     /locus_tag="OO315_002463"
                     /pseudo
     CDS             5952..7611
                     /gene="fliF"
                     /locus_tag="OO315_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            7604..8599
                     /gene="fliG"
                     /locus_tag="OO315_002464"
     CDS             7604..8599
                     /gene="fliG"
                     /locus_tag="OO315_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5891339.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            8592..9278
                     /gene="fliH"
                     /locus_tag="OO315_002465"
     CDS             8592..9278
                     /gene="fliH"
                     /locus_tag="OO315_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5891340.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            9278..10651
                     /gene="fliI"
                     /locus_tag="OO315_002466"
     CDS             9278..10651
                     /gene="fliI"
                     /locus_tag="OO315_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5891341.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            10673..11014
                     /gene="fliJ"
                     /locus_tag="OO315_002467"
                     /pseudo
     CDS             10673..11014
                     /gene="fliJ"
                     /locus_tag="OO315_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            11011..12138
                     /gene="fliK"
                     /locus_tag="OO315_002468"
     CDS             11011..12138
                     /gene="fliK"
                     /locus_tag="OO315_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5891342.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            12243..12707
                     /gene="fliL"
                     /locus_tag="OO315_002469"
     CDS             12243..12707
                     /gene="fliL"
                     /locus_tag="OO315_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5891343.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            12712..13716
                     /gene="fliM"
                     /locus_tag="OO315_002470"
     CDS             12712..13716
                     /gene="fliM"
                     /locus_tag="OO315_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5891344.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            13713..14126
                     /gene="fliN"
                     /locus_tag="OO315_002471"
     CDS             13713..14126
                     /gene="fliN"
                     /locus_tag="OO315_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5891345.1"
                     /translation="MRDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            14129..14494
                     /gene="fliO"
                     /locus_tag="OO315_002472"
     CDS             14129..14494
                     /gene="fliO"
                     /locus_tag="OO315_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5891346.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            14494..15259
                     /gene="fliP"
                     /locus_tag="OO315_002473"
                     /pseudo
     CDS             14494..15259
                     /gene="fliP"
                     /locus_tag="OO315_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            15269..15538
                     /gene="fliQ"
                     /locus_tag="OO315_002474"
     CDS             15269..15538
                     /gene="fliQ"
                     /locus_tag="OO315_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5891347.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            15547..16332
                     /gene="fliR"
                     /locus_tag="OO315_002475"
     CDS             15547..16332
                     /gene="fliR"
                     /locus_tag="OO315_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5891348.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            16617..17240
                     /gene="rcsA"
                     /locus_tag="OO315_002476"
     CDS             16617..17240
                     /gene="rcsA"
                     /locus_tag="OO315_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5891349.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(17284..17472)
                     /gene="dsrB"
                     /locus_tag="OO315_002477"
     CDS             complement(17284..17472)
                     /gene="dsrB"
                     /locus_tag="OO315_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5891350.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            17635..17862
                     /gene="yodD"
                     /locus_tag="OO315_002478"
     CDS             17635..17862
                     /gene="yodD"
                     /locus_tag="OO315_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5891351.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            18160..18975
                     /locus_tag="OO315_002479"
                     /pseudo
     CDS             18160..18975
                     /locus_tag="OO315_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(18972..20666)
                     /gene="yedQ"
                     /locus_tag="OO315_002480"
                     /pseudo
     CDS             complement(18972..20666)
                     /gene="yedQ"
                     /locus_tag="OO315_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(20837..21019)
                     /locus_tag="OO315_002481"
     CDS             complement(20837..21019)
                     /locus_tag="OO315_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5891352.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(21098..22015)
                     /locus_tag="OO315_002482"
     CDS             complement(21098..22015)
                     /locus_tag="OO315_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5891353.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            22188..23108
                     /gene="yedA"
                     /locus_tag="OO315_002483"
     CDS             22188..23108
                     /gene="yedA"
                     /locus_tag="OO315_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5891354.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(23097..23567)
                     /gene="vsr"
                     /locus_tag="OO315_002484"
     CDS             complement(23097..23567)
                     /gene="vsr"
                     /locus_tag="OO315_002484"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5891355.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(23548..24966)
                     /gene="dcm"
                     /locus_tag="OO315_002485"
     CDS             complement(23548..24966)
                     /gene="dcm"
                     /locus_tag="OO315_002485"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5891356.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(25033..25573)
                     /locus_tag="OO315_002486"
                     /pseudo
     CDS             complement(25033..25573)
                     /locus_tag="OO315_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(25613..>25848)
                     /locus_tag="OO315_002487"
     CDS             complement(25613..>25848)
                     /locus_tag="OO315_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891357.1"
                     /translation="AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLG
                     ALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         6517 a         6630 c         6368 g         6333 t
ORIGIN      
        1 tcgccagcgt cttctggttt ttctgaaaac tgtttacagg ctcaaagact gtgccatctg
       61 acagcgaggc aagtttagcc acgccagcat ccagcccgac cattgatgct gaagggtgaa
      121 ccggagttga tacttcactt tctgcctgaa tactgatgta ccacttaccg caggactggc
      181 tgacagtgac atttttcaca acacccgtga cctgacggct gttccggtag cgcatccagc
      241 caagttttgg cagaaaaata cggctgtttt cctgagcgag cttaacgccc tgcggatagc
      301 ggaatgcatc attctgtcac cgctttttga atcaggggaa cgccgagcgc ttctggaaaa
      361 agtttttgca ggcctgctca gggtctttca gtgactgttg caatggctgt gacggagcat
      421 ctttaatcca ttgcgtttca gtggcatttt tccactcaac caatcaggaa gccattttcg
      481 cgtaagggat gtctttgtta ccggccttat gattctcatt ctgacgtgcc agagtacgac
      541 tgaaaacgaa acgacatgcg cctgcgaagc gcctcatttc acgcgcctgt tgaccaccgg
      601 gtcttaaccg gaatttgaat acctgtagtc gtttcacagg gcaaattata ctttggtctg
      661 tgaaaaaaac tgatatccga cgcggaaggc actgtgtttt cctgatgcat gctcaccggg
      721 tctttgttac caagtaccga cgccagattt ttgaccatga cgcgacagaa aaactacgca
      781 cttacttttc aaatgtatgt gcttattttg aagctgaact ggttgaaatg gatggcgaac
      841 cagatcacgt ccatctgtta attaactatc cccccaaact ggcgatatcc agtctggtaa
      901 acaaacagcc tcaaaggcgt atcgggtagg ttactgcgac gggatcgacc agatattgct
      961 gccaggtatt accgcaaaga tgttttgtgg cgtcccggct attttgccag tagctgcgga
     1021 ggtgcgccaa tatccgtcat ccgccaatac attgaacaac agcaaacacc tggtcagatg
     1081 aaaaaacgag ccttatatcc ccgccctgaa ggacgggctt ttacggtgca ctggataagc
     1141 ctggagatta tctacacctg catggacatc attgtacgat gccccgtaca tgctccatca
     1201 catcctcaac atcaaagtat tagagccttg caaaccatta tgcgtctcag tttgtctcat
     1261 ctatcttacg caaaaaaaat agaactaata tcaagcggcg ctatattgcg tattaaataa
     1321 aaccacctca attacattaa taaatctaaa taaatatcat gcaaataatt tccatggtta
     1381 tttttaggct atttaaatta aaaaaataaa ttatgagtca atcataaata acaaccttta
     1441 atataatttg actaccaaat ttatattcgt tcacaaaata gtcatgcatt gtgtgcaata
     1501 gaaacagttc agataaaaat agggatctac ttcacaattt aaatgagaaa ctaaaactta
     1561 catcttgaaa taatctcatt gattggatga atatttgtag tgcagtgaca tcatttttaa
     1621 ataatagttc aaataaaagg gctcacgatg aaaaaattaa cagtggcaat ttctgctgta
     1681 gctgcatcag tactgatggc gatgtctgct caggcagcag agatttacaa taaagacagt
     1741 aacaagctgg atctgtacgg aaaagttaat gctaagcact acttctcttc taacgatgca
     1801 gatgatggcg acacaactta tgttcgtctg ggcttcaaag gcgaaactca aatcaacgat
     1861 caactgactg gtttcggtca gtgggaatat gaattcaaag gcaaccgtgc tgaatctcaa
     1921 ggttcttcca aagataaaac ccgtcttgcc ttcgctggcc tgaaattcgg tgactacggc
     1981 tccatcgatt atggccgtaa ctacggtgta gcatacgata tcggtgcatg gaccgacgtt
     2041 ctgccagaat tcggtggcga tacctggacc caaacagatg tgttcatgac tggtcgcacc
     2101 acaggtgttg caacctatcg taacaacgac ttctttggtc tggttgatgg tctgaacttt
     2161 gctgctcagt atcagggtaa aaatgaccgc actgacgtaa ctgaagctaa cggtgatggt
     2221 ttcggtttct ccactactta tgagtatgaa ggattcggtg taggtgcaac ctatgctaaa
     2281 tctgaccgca ctaatgatca ggttatctac ggtaacaaca gcctgaatgc atctggtcaa
     2341 aatgctgaag tatgggcagc tggtctgaaa tatgatgcga acaacatcta tctggctacc
     2401 acctattctg aaacccagaa catgactgtt tttggtaata accatattgc caacaaagca
     2461 caaaacttcg aagtagttgc acaatatcag ttcgacttcg gtctgcgtcc gtccgttgct
     2521 tacctgcaat ctaaaggaaa agacttgggt gcgtggggtg atcaggacct gattgaatat
     2581 attgatgtag gtgcaaccta ttacttcaac aaaaatatgt ccacttttgt tgattacaaa
     2641 atcaacctga ttgataagag cgatttcacg aaagcatctg gcgttgctac cgatgatatc
     2701 gttgctgtag gattggttta ccagttctaa tctgattact caaagatatg ttgtgggagg
     2761 ctttgcctcc ccaacatata agtggcgctc tcaagccact ttccttagaa gcactatctt
     2821 gcttctgact atataaatta ccctttattt tctgggacgt ttcaacgccc catttttaat
     2881 aatactcagt gaacaattaa cgtatcaatt agatttaata acaacgatat ccgtacctaa
     2941 cctgatatct aatgtcataa gcatcccttt aattatcccc tcaacctctt gtaaatttcc
     3001 cactcttgag ctccacatag cccaaacgcc ggatgcaaaa aaaatcatac gtgcatctct
     3061 gaaattgtga tggaggaagt gagtatcaat tatatgtgct tataaacgtt cccgattggc
     3121 acagaagtca ccggattgtt caggctccaa taggttgagt atgtcgtatt tattttccaa
     3181 aataaaaatc gatgctgttg ctccttaata aaatactttt actctctgga cgcttttctt
     3241 agctctcttg gtgttatatt aaaacgatta tggaaccttt cagtaaaacg agaaggacac
     3301 ttgtaaccat tttccctggc aatctcgctg atgggtttta ctgtcgtttg tatagcagga
     3361 caacgcatta tttaacctca cgtcgtccaa tatactttga aaacttattc cctcgcttgc
     3421 cagacggcga tgcaatgtgg aaacagaaat gtagagatat ctcgctacct tgtttgctgt
     3481 ccattttgtt ctaggttcgg atagcattag gttataacaa cgacttatta atgattgttt
     3541 actatctgat aaaagtaaac aattaacatg atgtgctcct aatgaaagta gaacgcccat
     3601 tgctaagtgt tcctgaattt gcgttgagaa gcctcgggaa atagatgttt ttagttgctc
     3661 ccaacaatat attaactcgg ggttctgagg taagcagaaa cctgtcgtgt tacgaatttg
     3721 atcagttatc gcataaaatt tttgaaactt ttcaattaga tcaatgggat aataaagcat
     3781 ttctgcaaga taaagacctg cttctggata attcacaata taaaattcat atccacaggg
     3841 aaataatatt atctgattat tatcaacagt tagactatgc gtctcccaat tgataacttt
     3901 ttttccctga cggatacgac ataaagctgg cataagaggc ttaaccctgt gaatctcatg
     3961 atgtttatgc atccgtattt cttcaatctt taggttagac ttatctcttg ccagcatact
     4021 cttaccctat tttatctcat aaactggtgt tatctcagcg gttgcaattt tattggcatt
     4081 aagcatataa ccaactaacg ctccgctgga attagaatcc acaggaatct tatcagtttt
     4141 tagagcccat actttaaact ggtaatgatg gggtttatct cctttgggag gacatgctcc
     4201 accaaatcct gcataaccaa aatcatttcg gccttgaaca gcaccagtcg gaagttttgt
     4261 accatcacgt cttcctgcat cagtgggcaa atatgttaca gttgctggaa tattagcaac
     4321 agtccagtgc caccatccac tgcctgtagg agcatctgga tcatacacag ttacggcaaa
     4381 gcttttggtg ccttcaggag caccagacca ggttaatgag ggcgatgtat taccaccttc
     4441 acacccaaat ccagaaaata catgagacgt tgtaagttgc tctcctgttt ttatttcatt
     4501 actagtgacc tgaaatgctg cggcctgcgc agaaaatgtt atgaatgcca atacacttga
     4561 aacgataagt tttttcataa aaacctcatt gttgtgacct atcgttattt tatttgatgt
     4621 gccttgatct cattatgcat aaaggcgcaa tgatcgcgca aatgcaatca taattataaa
     4681 ggtgccccga acattagcca taatatgcac aaatcagatt cctgttatct caaggagaat
     4741 tattttttaa tgcggtgcac ttcgtgacaa caagttaatg actaacacac cggcacaaat
     4801 caacatcatg cctatgatag ctggcaggtc cagtcgttga ccaaaaaatc cccaggaaag
     4861 caaactaatc agaacgatac cgactcctga ccagatagca taagcaatcc ctgtaggtat
     4921 ataagccagc gtctgagcta ataaccagaa tgatgcacaa tagcaaataa ttgtgccaac
     4981 agagggccat aaccgtgtaa acccctctga aaacttcatt aaggttgtac caatgacctc
     5041 tgcaagtatt gcgccaccaa gataaatata agggttcata gcatattctt tcctgttcat
     5101 actggagaga attgtactac agtttgaaca caactcaccg ttttcatcct ggtgtatgtg
     5161 tatccaaggg gctctggcgt ggcaagagat aaaggcagga acaataatga tcagaaggaa
     5221 ccccaggcta acgaatgagt tcactcttcc tgggagcaaa gcgaagacaa aacatgttat
     5281 cgcgtttacg gatcgtggat ggcagaaaac aaccaggatc aggtatccat tttaaaccag
     5341 aaattgagtg agtttgcccc atccatgccc cacgctgtgg gatcggatat aattaaacaa
     5401 gcatagtcaa caggttacac aacctacacc tgcatggaca tcacttcctg atacgccgcc
     5461 accagcttat tacgcacctg aatccccatt tgcatagaaa ctgaggcttt ttgcatatcg
     5521 gtcatcacat cgtttagcgc cacgccgggt tcaccgaggg tgaatttttc tgcctgcgtg
     5581 cgggcgactg tttgtgtatc gcttatgcga tcgagcgcgg cgtgcagctg cccggcaaaa
     5641 ctaatggtcg gttgcggcag tgattcctgc gcacgcgcac tcatcgccgt agcctgtaac
     5701 tggctgataa ccccttcaat cccctgtatc gctgacattt tcgtctcccg gataatttct
     5761 ggtaacaaag cctaccagta agtcaataag acaaaggcgc taaatagcaa caaaaaaact
     5821 ggtttattgg cggatagaaa aaacgaaagc acaaataatg ggagcgtcaa tttttcgagt
     5881 gtgctgaccc gagagtgagt cttgttccac tttgccaata acgccgtcca taatcagcca
     5941 cgaagtgcgc gatgaatgcg actgcagccc agacaaaatc tcttgagtgg cttaatcgcc
     6001 tgcgtgcgaa tccgaaaatt ccattgattg ttaccggttc cgcggcagtg gcggtcatgg
     6061 tcgcactgat cctgtgggcg aaagcccccg actaccgcac attattcagc aatctttccg
     6121 atcaggatgg tggcgcaatt gtcagccaac tgacgcaaat gaatattcct taccgcttca
     6181 gcgaagccag cggcgctatt gaggttccgg cagataaagt tcacgaactg cgcctggcgc
     6241 aacaaggttt gcccaaaggc ggcgcggtcg gtttcgaact gctcgatcag gaaaagtttg
     6301 gtatcagcca gttcagcgaa caggtgaatt atcagcgggc gctggaaggc gagctttctc
     6361 gtaccatcga aactatcggc ccggtaaaag gggcgcgcgt acatctggca atgccgaaac
     6421 cgtctttatt cgtccgtgaa caaaaatccc cttctgcatc ggtgacggta aatctgttac
     6481 ccggccgcgc actcgatgaa gggcaaatta gcgctattgt acatctggtt tccagcgcgg
     6541 ttgctggtct gccgcctgga aacgtcacgc tggtggatca gggcgggcat ctgttaaccc
     6601 agtccaatac cagcgggcgc gatcttaatg acgctcagtt gaaatatgcc agcgatgtcg
     6661 aaggccgtat tcagcagcgt attgaagcga tcctgtcacc tattgttggt aacggtaata
     6721 ttcacgccta ggtcacggcg caactggact tcgccagtaa agaacaaacg gaagaacagt
     6781 atcgccctaa cggtgatgaa tctcatgcgg cgcttcgttc acgccagctt aatgagagcg
     6841 agcaaagcgg ttccggttat ccgggcggcg taccgggggc gttgtcgaat caaccggcac
     6901 ctgcgaataa cgcgccaatc agcacgcctc cgacaaatca aaataaccgc cagcagcagg
     6961 cgagcaccac cagcaatagt gggccgcgta gcacacagcg gaatgaaacc agtaactacg
     7021 aagtcgatcg caccattcgt cataccaaaa tgaacgtggg cgatgtgcaa cgtctgtcag
     7081 tcgcggtagt ggtgaattac aaaaccttgc cagacggtaa accattgcct ctcagcaacg
     7141 aacagatgaa gcaaattgaa gctctgaccc gcgaggcgat gggcttttct gaaaaacgcg
     7201 gtgattcgct caatgttgtt aactcgccgt tcaatagcag tgacgaaagc ggcggagcac
     7261 tgccgttttg gcaacagcaa gtgtttatcg atcagttgct ggctgccggt cgctggttgc
     7321 tggttgctgg tactgctggt ggcgtggttg ctgtggcgga aagcggtacg cccgcagcta
     7381 acacgtcgcg ccgaggcggt gaagactgtg cagcaacagg cgcaggcccg cgaggaagtg
     7441 gaagatgcgg tggaagtccg tctgagcaaa gacgaacaac tccaacaacg gcgcgctaac
     7501 caacgactgg gggcagaagt catgagccag cgtatccgtg aaatgtctga taacgatccg
     7561 cgcgtggtgg cgctggtcat tcgccagtgg ataaataacg atcatgagta acctgacagg
     7621 caccgataaa agcgtcatcc tgctgatgac cattggcgaa gaccgggcgg cagaggtgtt
     7681 caagcacctc tcccagcgcg aagtgcaaac cctgagcgct gcaatggcga acgtcacgca
     7741 gatctccaac aagcagctaa ccgatgtgct ggcggagttt gagcaagaag ctgaacagtt
     7801 tgctgcactg aatatcaacg ccaacgatta tctgcgttcg gtattggtca aagctctggg
     7861 tgaagaacgt gccgccagcc tgctggaaga tattctcgaa acccgcgata ccgccagcgg
     7921 tattgaaacg ctcaacttta tggagccgca gagcgccgcc gatctgattc gcgatgagca
     7981 tccgcaaatt atcgccacca ttctggtgca tctgaggcgc gcccaggccg ccgatattct
     8041 ggcgttgttc gatgaacgtc tgcgccacga cgtgatgttg cgtatcgcca cctttggcgg
     8101 cgtgcagcca gccgcgctgg ccgaactgac cgaagtactg aatggcttgc tcgacggtca
     8161 gaatctcaag cgcagcaaaa tgggcggcgt gagaacggca gccgaaatta tcaacctgat
     8221 gaaaacgcag caggaagaag ccgttattac cgccgtgcgt gaattcgacg gcgagctggc
     8281 gcagaaaatc atcgacgaga tgttcctgtt cgagaatctg gtggatgtcg atgatcgcag
     8341 cattcagcgt ctgttgcagg aagtggattc cgaatcgctg ctgatcgcgc tgaaaggggc
     8401 cgagcagcca ctgcgcgaga aattcctgcg caatatgtcg cagcgtgccg ccgatattct
     8461 gcgcgacgat ctcgccaagc gtggtccggt gcgtctgtcg caggtcgaaa acgaacagaa
     8521 agcgattctg ctgattgtgc gccgtcttgc cgaaactggc gagatggtga ttggcagcgg
     8581 cgaggatacc tatgtctgat aatctgccgt ggaaaacctg gacgccggac gatctcgcgc
     8641 caccaccggc agagtttgtg ccaatggtcg agtcggaaga aaccattatt gaagaggccg
     8701 aacccagcct tgagcagcaa ctggcgcaac tgcaaatgca ggcccacgag cagggttatc
     8761 aggcggggat tgccgaaggt cgccagcaag gtcacgaaca gggctatcag gaaggactgg
     8821 cccaggggct ggaacaaggt ctggcagagg cgaaggctca acaagcgcca attcatgccc
     8881 ggatgcagca actggtcagc gaatttcaaa ctacccttga tgcgcttgat agtgtgatag
     8941 cttcgcgcct gatgcagatg gcgctggagg cagcacgtca ggtgatcggt cagacaccaa
     9001 cggtggataa ctcggcactg atcaaacaga tccaacagtt gttgcagcaa gaaccgttat
     9061 tcagcgggaa accacagctg cgcgtacacc cggatgatct gcaacgtgtg gatgatatgc
     9121 tcggcgcaac tttaagtttg catggctggc gcttgcgggg cgatcccacc ctccaccctg
     9181 gcggatgtaa agtctccgcc gatgaaggcg atctcgacgc cagtgtcgcc actcgctggc
     9241 aagaactctg ccgtctggca gcaccaggag tggtgtaatg accacgcgcc tgactcgctg
     9301 gctaaccacg ctggataact ttgaagccaa aatggcgcag ttgcctgcgg tacgtcgcta
     9361 cgggcgatta acccgcgcta ccgggctggt gctggaagcc accggattac aattgccgct
     9421 cggcgcaacc tgtgtcattg agcgccagaa cggcagcgaa acgcacgaag tagaaagcga
     9481 agtcgttggc tttaacggtc aacggctgtt tttaatgccg ctggaggaag tggaaggtgt
     9541 cctgcccggc gcgcgtgtat atgccaaaaa catttcggca gaagggctgc aaagcggcaa
     9601 gcagttgccg ctcggtccgg cgttattagg tcgtgttctg gacggtagcg gtaaaccgct
     9661 cgatggcctg cccttccccg atacgacgga aaccggtgcg ctgattaccc cgccatttaa
     9721 cccgttgcaa cgtacaccga ttgaacatgt gctggacacc ggcgtgcgcc caatcaatgc
     9781 cctgcttacc gtcggacgcg ggcagcgtat ggggctgttt gccgggtcgg gcgttggtaa
     9841 aagtgtgctg ctggggatga tggcacgcta cacccgcgcc gatgtcattg tcgtgggttt
     9901 gattggtgaa cgtgggcgcg aagtaaaaga ttttattgag aatatcctcg gtgccgaagg
     9961 acgtgcacgc tcagtggtga ttgccgctcc ggcggatgtt tctccgctcc tgcgaatgca
    10021 aggtgccgcc tatgccacgc gcattgccga agattttcgc gatcgtggtc agcatgtgtt
    10081 gctgattatg gactccctca cccgctacgc gatggcccag cgtgaaattg cgctggcgat
    10141 tggcgaacca cctgccacta aaggttatcc accgtcggtg tttgccaaat taccggcact
    10201 ggtcgagcgt gccggtaatg gcattagcgg cggcggctcg attaccgcgt tttataccgt
    10261 gctcaccgaa ggcgatgacc agcaggaccc cattgctgac tccgcgcggg caatcctcga
    10321 cggtcacatt gtgctgtctc gccgactggc ggaagccggg cactatccgg ctatcgatat
    10381 tgaagcgtcg atcagtcgcg caatgacggc gttgatcagt gagcaacatt acgcgcgagt
    10441 gcgcaccttc aaacagctgt tgtcgagttt tcagcgtaac cgcgatctgg ttagcgtcgg
    10501 cgcgtatgcc aaaggcagcg atccgatgct cgataaagcc atcgccctgt ggccgcagct
    10561 ggagggctat ttgcaacaag gcatttttga acgcgcggac tgggaagcgt ctctccaggg
    10621 gctggagcgt attttcccga cagtgtcata acccaggaga taacggcaga tggcggaaga
    10681 acagctcaaa atgttgatcg attatcagaa tgaatatcgc aataacctca atagcgatat
    10741 gagtgccggg atgaccagca accgctggat caactatcag cagtttatcc agacgctgga
    10801 aaaagccatt actcagcatc gccagcaact taatcagtgg acgcagaaag ttgacattgc
    10861 cctgaacagc tggcgagaaa agaaacaacg tttgcaggcc tggcagacac tgcaggaacg
    10921 gcaatccacg gcggcactgc ttgcagaaaa ccgcctcgat cagaaaaaga tggatgagtt
    10981 cgcccagcgc gccgccatga ggaaacctga atgattcgct tagcgccctt gattaccgcc
    11041 gacgttgaca ccaccacatt gcctggcggc aaagccagcg atgctgcaca agattttctc
    11101 acgttgttga gcgaagcatt agcaggcgag acaacaactg acaaagcggc cccccagttg
    11161 ctggtagcaa cagataagcc cacgacaaaa ggcgagccgc tggtcagcga tattcttgcc
    11221 gatgcgcaac aagcggattt actgatccct gtggatgaaa cacttcctgt catcaacgac
    11281 gaacaatcca catcaacacc gttaaccacc gctcagacga tgacgttggc tgcggtggct
    11341 gacaaaaata cgacaaaaga cgaaaaagcg gatgatctta atgaagacgt caccgcaagt
    11401 ctgagcgccc tttttgcgat gttgccgggt tttgacaata cgcccaaagt gactgatgca
    11461 ccgtcaaccg tgttaccggc agagaaacca acgctcttca caaaactgac ttctgcgcaa
    11521 ctcactacag cacagcctga tgacgccccc ggcacaccag ctcagccatt aacaccgctg
    11581 gtagcagaag cccagagtaa agcggaagtc atcagcacgc cttcgccggt gaccgctgac
    11641 gccagcccgc taatcactcc acaccagaca cagccactgc ccaccgtcgc cgcgcctgtt
    11701 ttgagtgcac cgctgggttc tcacgaatgg caacaatcat tgagccagca tatttcgctg
    11761 ttcacccgcc aggggcaaca aagtgcagag ttgcgtctgc acccacagga tttaggtgaa
    11821 gtgcaaattt ccctcaaagt ggatgataac caggcgcaga tccagatgat ttcaccgcat
    11881 cagcacgtac gcgccgccct ggaagcagcg ctgccggtac tgcgtacgca gctggccgaa
    11941 agtggcattc agttagggca aagcaacatt agtggcgaaa gctttagtgg tcagcagcag
    12001 gccgcttccc agcaacagca aagccaacgc acagcaaacc atgaacctct ggcgggggaa
    12061 gacgacgata cgcttccggt tcccgtctct ttacaagggc gtgtaacagg taacagcggc
    12121 gttgatattt tcgcctaacg tcagaggtag caccgtaatc cgcgtctttt ccccgctttg
    12181 ttgcgctcaa gacgcaggat aattagccga taagcagtag cgacacagga agaccgcaac
    12241 acatgactga ttacgcgata agcaagaaaa gcaagcgatc gctttggatc ccgattctgg
    12301 tattcattac cctcgcggcc tgtgccagcg caggttacag ctactggcat tcgcatcagg
    12361 ttgccgctga cgacaaagcg cagcaacgcg tcgtgccctc cccggtcttc tatgcgctgg
    12421 atacctttac ggtcaatttg ggcgatgcgg atcgcgtgct ttatatcggc ataaccctgc
    12481 gactgaaaga tgaagcaacc cgctcgcggc tgagtgagta tttgccggaa gtccgtagtc
    12541 gcttgctgtt actgttttca cgtcaggatg ctgccgtact ggcgacagaa gaaggcaaga
    12601 aaaacctgat tgccgagatt aaaaccacac tttccacccc gcttgttgcc gggcaaccga
    12661 aacaggatgt caccgacgtg ctgtataccg cttttattct gcgataacga catgggcgat
    12721 agtattcttt ctcaagctga aattgatgcg ctgttgaatg gtgacagcga agtcaaagac
    12781 gaaccgacag ccagtattag cggcgaaagt gacattcgtc cgtacgatcc gaatacccaa
    12841 cgacgggttg tgcgcgaacg tttgcaggcg ctggaaatca ttaatgagcg ctttgcccgc
    12901 cattttcgta tggggctgtt caacctgctg cgccgtagcc cggacattac cgtcggggcc
    12961 atccgcattc agccgtacca tgaatttgcc cgcaacctgc cggtgccgac caacctgaac
    13021 ctaatccacc tgaaaccgct acgcggcaca gggctggtag tgttctcgcc gagtctggtg
    13081 tttatcgccg tggataacct gtttggcggc gatggacgct tcccgaccaa agtggaaggt
    13141 cgcgagttta cccatactga acagcgcgtc atcaaccgta tgttgaagct ggcgctggaa
    13201 ggctatagcg acgcctggaa agcgattaat ccgctggaag ttgagtacgt gcgttcggaa
    13261 atgcaggtgg aatttaccaa tatcaccacc tcgccgaacg acattgtggt taacacgccg
    13321 ttccatgtgg agattggcaa cctgaccggc gaatttaata tctgcctgcc attcagcatg
    13381 atcgagccgc tacgggaatt gttggttaac ccgccgctgg aaaactcgcg taatgaagat
    13441 cagaactggc gcgataacct ggtgcgccag gtgcagcatt cacagctgga actggtggcc
    13501 aactttgccg atatctcgct acgcctgtcg cagattttaa aactgaaacc cggcgacgtc
    13561 ctgccgatag aaaaacccga tcgcatcatc gcccatgttg acggcgtccc ggttctgacc
    13621 agtcagtacg gtactctcaa cggtcagtat gcgttacgga tagaacattt gattaacccg
    13681 attttaaatt ctctgaacga ggaacagccc aaatgagaga catgaataat ccggccgatg
    13741 acaacaacgg cgcaatggac gatctgtggg ctgaagcgtt gagcgagcaa aaatcaacca
    13801 gcgaaaaaag cgctgccgac gcggtgttcc agcaatttgg cggtggtgat gtcagcggaa
    13861 cgttgcagga tatcgacctg attatggata ttccggtcaa gctgaccgtc gagctgggcc
    13921 gtacgcggat gaccatcaaa gagctgttgc gtctgacgca agggtcagtc gtggcgctgg
    13981 acggtctggc gggcgaaccg ctggatattc tgatcaacgg ttatttaatc gcccagggcg
    14041 aagtggtggt tgttgccgat aaatatggcg tgcggatcac cgatatcatt accccgtccg
    14101 agcggatgcg ccgcctgagc cgttagtgat gaataaccat gctactgtgc aatcttccgc
    14161 gccggtttct gctgagccac tgctgcaggt gagcggcgca ctcatcgcca ttattgccct
    14221 gatcctcgct gctgcctggc tggtaaaacg gctgggattt gcccctaaac gcactggcgt
    14281 taacggtctg aaaattagcg ccagtgcttc gctgggcgcg cgtgaacggg tcgtggtggt
    14341 cgatgtggaa gatgcacggc tggtgctcgg cgttaccgca gggcaaatca atctgctgca
    14401 taaacttccc ccttcagcac caacggaaga gataccgcag accgattttc agtcggtcat
    14461 gaaaaatttg cttaagcgtg gcgggagatc ctgatgcgtc gtttgttttc tgtcgcaccg
    14521 gtccttctct ggctgattac gcccctcgcc ttcgcgcaac tgccgggtat caccagccag
    14581 ccgctacctg gcgggtatca ccagccagcc gctacctggc ggtggacaaa gctggtcgct
    14641 cccggtgcag acgctggtgt tcatcacctc gttgacgttc attccagcga ttttactgat
    14701 gatgaccagt ttcacccgca tcattattgt ttttggttta ttgcgtaacg cgctgggaac
    14761 gccctccgcg ccacctaacc aggtattgct ggggctggca ctgtttttga ccttttttat
    14821 tatgtcgcca gtgatcgaca aaatttatgt cgatgcgtac cagccattca gcgaagagaa
    14881 aatctcaatg caggaggcgc tggaaaaagg ggcgcagccg ctgcgtgagt ttatgctgcg
    14941 tcagacccgc gaggcagatt tagggttgtt tgccagactg gcgaataccg gcccgttgca
    15001 ggggcctgaa gctgtgccga tgcgcatttt gctcccggcc tacgtgacca gcgagttgaa
    15061 aaccgcattt cagataggct tcacgatttt catccctttt ttgattatcg acctggtgat
    15121 tgccagcgtg ttgatggcat tggggatgat gatggttccc cccgccacca ttgctctgcc
    15181 ctttaaactg atgctgtttg tactggtgga tggctggcaa ttgctggtcg gttcgctggc
    15241 gcagagcttt tacagctaga gaggcaaaat gacacctgaa tcggtcatga tgatgggaac
    15301 tgaagcgatg aaagtcgcgc tggcactggc agccccgctg ttgctggtag cgctggtcac
    15361 gggccttatc atcagtattt tgcaggcagc cacgcagatt aacgaaatga cgctgtcgtt
    15421 tattccgaaa atcatcgccg tatttatcgc catcattatt gccggaccgt ggatgctcaa
    15481 tctgttgctg gattacgtcc gcaccttgtt cactaacctg ccgtatatca tcgggtagcc
    15541 cgtacaatga tgcaggagac aagcgatcaa tggctttcct ggttaagcct gtacttctgg
    15601 ccgttactgc gcgtgctggc gctcatttct accgcgccaa ttctgagcga acgcagcgtg
    15661 ccgaaacggg taaaactggg tctggcaatg atgatcacgt tcgccattgc cccatcatta
    15721 cctgccaacg atgttcctgt tttttcgttc tttgctctgt ggctggccgt acagcagatc
    15781 ctgatcggca ttgcgcttgg ttttaccatg caatttgcct ttgccgctgt gcgaaccgct
    15841 ggcgaaatta tcggtctgca aatggggctg tcatttgcga catttgtcga tcctggcagc
    15901 catctcaata tgcccgtttt agcgcgcatc atggatatgc tggcattact gctgttcctg
    15961 acatttaacg gtcatttatg gttgatttca ctgctggtcg atacctttca caccctgccg
    16021 attggtagcg aaccgttgaa cagcaatgcg tttctggcac ccaccaaagc agggagtttg
    16081 attttcctta acgggctgat gctggcgtta ccgctcatta ctctgttgct gaccctgaat
    16141 ctggcattag gtttactgaa tcgtatggcc ccgcaattat ccatttttgt tattggattt
    16201 ccattaactc tgactgtcgg catctcttta atggcggcat taatgccgtt aattgcgcct
    16261 ttttgcgaac atttattcag tgaaattttt aatttgctgg ctgatattat tagtgaatta
    16321 ccattaatat aattccgtaa cgtttatcat gctaccctaa ggattatccg aaaaataata
    16381 tctgcgaaca tcttccagga tactcctgta gcgaaatatt tgttttaagc tcactcacat
    16441 atcgcaacat ttactttaag acaattccag gcaaattata caacacttta cgggatagta
    16501 agtccgcctg aaaaattgcg agagtggcgc attaggtgac ccatgttgtt ccgtttagtc
    16561 atgatgaaat attcaggtaa ggggaattat cgttacgcat tgagtgaggg tatgccatgt
    16621 caacgattat tatggattta tgtagttaca cccgactagg tttaaccggg tatctgttga
    16681 gtagaggggt taaaaaaaga gaaatcaacg acattgaaac cgttgatgac cttgccatag
    16741 cttgtgattc acagcgtcct tcagtggtgt ttattaatga ggactgtttc atccacgatg
    16801 cttctaacag tcagcgcatc aagcacatca ttaatcaaca tcccaatacg ttatttatcg
    16861 tttttatggc aattgccaat gttcattttg atgaatatct attggtcaga aaaaatttat
    16921 tgatcagttc taaatcgatt aaaccggaat ctctcgacga tatccttggc gatattctga
    16981 aaaaagagac aacgataacc tcgtttttaa atatgccgac gttatcattg agccgaaccg
    17041 aatcgagtat gttgcgaatg tggatggcag gtcagggaac cattcaaatc tccgaccaaa
    17101 tgaatatcaa agccaagacc gtttcatcgc ataaaggtaa tattaaacgt aagatcaaaa
    17161 cgcataataa acaggttatc taccatgtcg tccgactgac ggataatgtg actaatggta
    17221 tttttgtcaa catgcgctaa cacattctga ctggtggttt cccaccagtc aggctgaata
    17281 agattactct gctttctcaa caaagatacc gtcctgatgc cctgcttcat taaagaacca
    17341 gatgccgagc gggtagtctt ccagcgcaac caggtacatt gtgccttcac taaactcctc
    17401 aactgccagt accactccag gacgacgcgg accgccatcc gttttgactg ttacccgatc
    17461 attcaccttc atcgttttcc tcctgtggct ttgtgccagt gtagaacaat ttcgtttttc
    17521 tggcagcgcc aagcgcgcgc gagtgctgat tttctcgacg gtctatactt aagagatgcc
    17581 aggcggactt aacgactggc ggcaacaaca gagtaacggt tgcgaggaaa gatgatgaaa
    17641 accgcaaaag tgtacagcga taccgcaaaa cgtgaggtca gcgtcgatgt cgatgccctg
    17701 ctggcggcga tcaatgaaat tagcgaaagc gaagttcatc gcagccagaa cgattctgaa
    17761 cacgttagcg tcgatggacg tgaatatcat acatggcgtg aattggcgga tgccttcgaa
    17821 ctggatattc atgacttcag cgtctctgaa gtgaatcgtt gaatgcacaa taaaaaaatc
    17881 ccgaccctga gggtgtcggg atgaaacttg cttaagcaag aagcacttaa aaaattcgtt
    17941 acaccaggaa atctgatgtg ttcatcacct tatctgcaat ttttttcgct gacaagaaaa
    18001 tattcgcgct atgaatgatt aacgctactt atgaatagaa atgtgacgcg cgtcacccct
    18061 tggtggctct ggtgtcaacg ggatcgctcc cgaaaaaatc atctttgatt atttgtgctg
    18121 ttttgagaat catcctggta aggggagtac tgcgcgacca tgttttcaat tcaacaacca
    18181 ctactggttt ttagcgatct tgatggcacc ctgctggaca gtcatagtta tgactggcaa
    18241 ccggcagccc cctggctcag ccgtttacac gaagccaata ttcccgtcat tctctgtagc
    18301 agtaaaactt tagcggaaat gctgtacttg caaaaaatgt tggggctaca aggcttaccg
    18361 ctgattgcag agaatggcgc agtgatccag cttgctgaac aatggcagga tatagacggt
    18421 tttccacgca tcatctcagg tattagccat ggcgaaatct gccaggtttt aaatacgcta
    18481 cgcgagaaag aacattttaa attcacgact tttgatgatg tcgacgatgc aaccattgcc
    18541 gaatggacgg gattaagccg tagctaggcg gcgctgacgc agctgcatga ggcgtcggta
    18601 acgctaatct ggcgcgacag tgacgagcgt atggcacaat ttatcgctcg tctgaacgaa
    18661 ctgggcttgc agtttatgca gggtgcgcgc ttctggcacg tactggatgc ctctgccgga
    18721 aaagatcagg ctgccaactg gattatcgcg acctatcaac aattgtcagg cagacgccca
    18781 accacacttg gcctgggcga tgggccaaac gatgcgcctt tactggaggt aatggattac
    18841 gcggtgattg tgaaagggct aaaccgtgaa ggggtacatc tgcatgatga ggatccggcc
    18901 cgcgtctggc gaacgcagcg tgaaggaccg gaaggctggc gtgaagggct ggaccatttt
    18961 ttctccgccc gttaagcgtt atcgctcgcg aatacccgat tgcgcccggc ctgttttgcg
    19021 aggtaaagcc gacggtcggc caatgactgg agttgttcaa aatcataatc accggtttcc
    19081 tcgctgctac ttacccccag cgaggcactg atgcgtatcg ttgtactctt ggcgatcagc
    19141 atctcttttt catttaactt caggcgaata cgttctgcga cctccgcagc ctgcgtcaga
    19201 ttcgcgcctg gcagaatcac acaaaactcc tcaccaccga cccgcccggc aacgtcctgc
    19261 gcacgcaagg aactgctaat taatccggca gcatgagaaa gaacacggtc gcccgcctga
    19321 tgaccaaagc ggtcattaat cgctttaaaa tggtcaagat cgacctgaat gacagaaaaa
    19381 ggatgttggt gcgtctgaca caatttagcg agcggacggg ctttttcgaa cagtgcgcca
    19441 cggttatata aacgcgttaa ggtgtcgtgc cacgcctgcc actgcaacga gctttgcaga
    19501 acatacatgt tgctgaccat ccggcgaatc acataccagg agaggagtaa catggtggta
    19561 aagagcgccc acagcagggt taatgcaatg ctgatactgc cgaaatcgcc gcgcacgcct
    19621 tcgcttagcg tatggacgcg cgccagcaca ccgtcgaaat ggtccagacg ttcccagcta
    19681 acatagcgac tgtccatacg aatgccgccc cgcgtgtcat gttccattgc ctgcgccagc
    19741 aaggccagtt cacgaggatc aaaaatattc cctgttggat ggtcaggatt ggaagaggtc
    19801 aaaaatctca gcttactgtc atagagctga tactcaccat cgaggttttt atcgatggcg
    19861 tttcttaaaa attgctgcat agtacgcacg ggaatactca tccccagcac gccataccag
    19921 tagttattac tgtctaccgg aacactgacg gtaacctgcg gttcagtatt gctggcgtgt
    19981 tccggttgcg aagtgaacca gcgtaccgcg cggtgacgat tttctcgttg cgaatggccg
    20041 ataaaccagg gttgggtgac atagccgtaa taacgcgttg gtacattgcg cgtaaacaag
    20101 gtcggctgcg tcgaaacgta aaatccagca cgcgagacat acatcgcctg ttcaaccatc
    20161 gacgaggagt tgtgcgccag tcgcagcaag taaccaactt ccagtgcagc ggtaatttca
    20221 ttgtcgaggc tttcattttc gcgagacagg aaattcccct cgctgactaa tgcatccgat
    20281 acgccattga ctggcagggt gcgccgtcgg ttgagttcga tttgccaggc gtgctcgtcg
    20341 cgatgctgtt cgaactcggt tacggcatta cgcagagagg tgaaatccag tggcgccacg
    20401 agagcttcgc gcatgccatt acgcaaaaag atcagtttgt cgacgttata ctgcaaatgc
    20461 ttatcgagcg cgttggctac gttttccaga tgattacgct agctggagat ataggcatct
    20521 tccagcacca ccacttcacg ccaggtgagc aaggtggaaa aaagcaatac cacgataaag
    20581 cagagattaa cgacatgacc gggccccagg cggcgtgcga gttttttcaa ccagctctgg
    20641 ttttccattt ttgtctcgtg ctgcaccctg acgactccca acctgctttt ttatgattct
    20701 ggcataaccc ggcaatagcg taccgcaaaa cagcgataat tttataatgg tggcatgata
    20761 agacgcgact atcattcatt gcaggcattg ggacaccagg ccggatgtga tccggcctgg
    20821 tgttaatgat accctttcaa acttcttccg cagaacgact cccctccccc ggaacaagat
    20881 cggtttcatg aaaagcttcc cgcttgaccc cgtaaccgtc ataccaacga cactcaacca
    20941 taccgctgga gtatccagtg acaatcatcc gcgggccgcc ctctttaacc gtaacttcct
    21001 cactaaccat aaagctcata ctcgcctcct tttttcgagt gaaacttgtt caccttagtt
    21061 gaagatggcg aattttgcat aatagccagt gcgagtatta atgtgcctga ccgcgcattt
    21121 tcgctactgc tttcactccc agcaccacga tgccgccgat aataaatcca agaatcagat
    21181 ttaaaacagt cggtaatatc atcgccacca ctgcactttg ctgcccggcg atatgttcaa
    21241 tagcgtgatg cagcggcgca ataccatgta ccacaatccc gccgccgacg aggaacatcg
    21301 ccagcgtgcc gacaatcgat aatgctttca tcagccaagg cgcgataatc aataaccctt
    21361 tacctaatgc ctgcatcagc gcgctagatt tttccgccag ccaataccct aggtcatcaa
    21421 tcttaacgat aacccctacc agaccgtaga cgcccacggt cactaccagc gcgatgcctg
    21481 aaagcaccag cacctgatta agcaacggcg cctctgccac aatccccagc gtgatggcga
    21541 cgatttccgc agacaaaata aaatcggtac gaatcgcccc tttaattttg tccttttcaa
    21601 acttcagcgg atcctgcgcc gccagcttct ccagacgctg ctggctctgc gctggatctt
    21661 ctttatgttt acgcgcctcc agcatatgca gcactttctc taccccttca aagcagagaa
    21721 acgcgccacc aatcatcaac agtggcgtaa tcgcccacgg gataaacgca ctgatgatca
    21781 gcgccagcgg caccagaatc actttattga tcagcgatcc tttcgccacg ccccagacca
    21841 cgggaagttc ccggttggcc cgcacgcctg aaacttgttg cgcattgagc gataaatcat
    21901 cccctaatac accggcggtt ttcttcgccg ccagtttgcc catcacagag atatcgtcca
    21961 gcagtgtggc gatatcatcg agcaacgtca gtaaactact cccggccaaa agcattccct
    22021 ctcattttta ttgttgaagg gtgtaagtat gaagcaaaaa cggcagctct gatacttgct
    22081 tcacaggtca acatttattt aacaatcaag tgcaattata tctctcgcca gcagaatgat
    22141 tttcacgttt actatgaacc acctttttat ttccgccgtg agggattatg cgtttccgcc
    22201 agttgttacc gctttttggc gcgctgtttg cgttgtatat catttggggc tcaacctatt
    22261 ttgtcattcg gattggcgtg ggaagctggc ctccgttaat gatggcgggc gttcgattcc
    22321 aggcagccgg tattttattg ctggcatttt tgctactgcg cggacacaaa cttcccccgc
    22381 tacgtccgct gctcaatgcc gcgctgattg gcctgttatt gctggctgtc ggtaatggca
    22441 tggtgacggt tgccgaacat caaaatgttc cttccggcat cgccgccgta gtgattgcaa
    22501 ccgtgcccct ctttaccctg tgcttcagtc gcctgtttgg cattaaaacg cgcaaactgg
    22561 aatgggtggg tattgccatt gggcttgccg gaatcatcat gctcaatagc ggtggaaatt
    22621 taagcggcaa tccgtggggc gcgattctga ttttaatcgg ctcgattagc tgggcgtttg
    22681 gctcagttta tggctcgcgc attaccttac ctgtagggat gatggcgggt gcgattgaga
    22741 tgctggcggc aggcgtggtg ttaatgatcg catcgatgat tgcgggtgaa aaactgacgg
    22801 cgctcccttc cctgtcaggc ttccttgcgg tcggctatct ggcgctgttt ggttcgatta
    22861 tcgccattaa cgcttatatg tatttaatcc gtaatgtcag tccggctctc gccaccagct
    22921 acgcctacgt taacccggtg gtcgcggtct tgctgggtac gggactgggt ggcgaaacgc
    22981 tgtcgaagat tgaatggctg gcgctcggcg taattgtttt cgcggtggta ctggtcacgt
    23041 tgggaaaata tctcttcccg gccaaacccg ttgtcgcgcc agttattcag gatgcatcaa
    23101 gcgagtaaat gaatcccctg cgtgtcgatc tgcgcgctgg caccttcgcc gcagatccac
    23161 tcttccagac gctcggtaag cgccgcatcc gtcagcttct cgcgcctacg taacgcacac
    23221 tcccagacaa tcagtacgcg ccagccgagc tcctgcaagc gactgatatc gcggcgatcg
    23281 cgttcaacat ttttacctat cttctccagc cagaactcag ttcgagtcgc aggcacttta
    23341 aacagatagc agtgatgatg atgccagaag cagccgtggg taaatatcac gcagcgatat
    23401 tcatcaacga caaaatccgg gcgtccgggc agactggcgt cctgaacgcg aaacgcaagg
    23461 ccctgcccgg ttaacaaact ggcgaggcgc ttctcaatcg ccgtatcacg cgtggcaatc
    23521 gcgcgcatat ttttgctgcg ggtggcctta tcgtgaacgt cgaccatgtt gtgcctcttg
    23581 ctgacgcaac gccaccgcct gtttgatttt tggctcaagc agttttgcca ccgcggcaaa
    23641 gaccggcacg accaccgagt tgccgaactg gcgataggcc tgagtgtccg aaaccggaat
    23701 gcggaatttc gcttctcccg gcgcttcaaa acccattaag cgcgcgcatt cacgaggcgt
    23761 taaccgacgt ggacgatgtt gctgattcag cggatcgtca aagtctttct cacccgtggc
    23821 catatcccaa ccgcgatcga ttaaaatttc cgcgccatct ttgtaataac gagcagacag
    23881 cgtacgggtg acgctttgcg gattgttcgg ataaaccatt ccataaccga agccgttacc
    23941 gcgcgcctga tgttttttcg catatcgata gaggtacttc cacagcaccg gcgtcaggat
    24001 atatttcgcc tcaaccatcg ggtccagcag ctgcgccagc gtcactcgct gtgcagggaa
    24061 acattcgctg atatcacgca gagtaaaatc ggctttcaga ttcagatcgc gacgaaaacc
    24121 caccagcacg atgcgttcac ggtgctgcgg caggaaatgt ttaccatcga tgattttcgg
    24181 atcgtccggc ccgttatctt ctgcatcggc aacgtcatag cccagttcgt ccagcgtctg
    24241 catgatgatg cggaacgttt ttcccttatc gtgactcttc aggtttttga cgttttcgag
    24301 cacaaacatc gccggacgac gcgcgtcgat aatgcgcacc acatcaaaga acagcgtgcc
    24361 ctgggtatcg caggcaaaac cgtgcgcccg cccgagcgag ttctttttcg atacgccagc
    24421 cagcgaaaat ggctgacaag ggaaaccggc cagtaaaaca tcgtgttcag gaatgtgttg
    24481 acgaatatgt tccgccgccg cctcatcact cacgccttct tgatgactga gggtgatgtc
    24541 gcggatatct tcattaaaat gatgcgtcgc cgggtcgcaa taatggttgg ctttataagt
    24601 gcgtaccgca tgtttgttcc attcgctggt aaacacgcac tgtccaccaa tcgattcaaa
    24661 accacgacgg atgccgccaa ttccggcaaa tagatcgata aagcgaaacg cataatgcgg
    24721 atgatgttcc ggtggtttgg gtaacaacgt ttgcagatgg gcaaactctt tctcacttaa
    24781 acggtgccat gccgagtcat tcgccagcgc acgttttaaa attgccgcgc tccagtgatt
    24841 ctcacccaca ccattaagct gcgccaccag cattttaaca tcataaattt gcagcagttt
    24901 ctccagcatt gcctgtgcgg cattcccggt gctgtatgaa tcggttactg atatattttc
    24961 ctgcatagat ttaaccggcc atctgagatg gccgacaggt taacataatt actgccgttt
    25021 agggccacgc gctcagccag cgggtgaaaa cgcatctatc accttgtgat cgataccttc
    25081 attctccccc gccagttcgg caccgagctt cgccatcaaa cagcgccaag ctcaatgccc
    25141 ctgaaacggc aaatacgcgc gccaggccaa tcgcccccag ggcttccagc cgatccgcat
    25201 cctggacgat tttagcttcc gttgttaagg gggcgatttg cgcgctgaaa ctgtgagcgg
    25261 caatggcatg acaaacggcc tcaattttct ccgccggaaa ttgcacgaac tcttcacgga
    25321 gcaggcgacg cgtctcttct gccgccagga ttgaagaacg ctgccgttgc ggatgatttt
    25381 tcgccaggct cacgatatcg tgaaaataac aggcggttaa aatcaccagc atatcgacat
    25441 cgtcatctgc cgccagtttc tgtgccgtag cccagacacg gcgaaaatga cacaggtcat
    25501 gcgccgcgtc ctgatgttga tgatggtttt tcaaccagtt ctcgaactat gcctgccagt
    25561 gttgtaactc catacgcgct ccgttgccaa agagtctcaa ccttagcagt ttttattcat
    25621 agcgtctgca acgtgcggga cgaggtcggg tgtaccatgc aatgccgcct aacaaggcac
    25681 caaccagggc cataaacaga aatccgacca atgctcctaa ccacttcccc gcggtagatc
    25741 ctaaatcact ttcaaatccc gacacggggg catcggggat ccggctcatc acccagatgt
    25801 atcgcgccat tgcccagacg aaatagccac accatgtata gaaagccc
//
LOCUS       DAJTRD010000056        25685 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTRD010000056 DAJTRD010000000
VERSION     DAJTRD010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.056"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(20..1384)
                     /gene="yeaG"
                     /locus_tag="OO315_002488"
                     /pseudo
     CDS             complement(20..1384)
                     /gene="yeaG"
                     /locus_tag="OO315_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
     gene            1820..2566
                     /gene="mipA"
                     /locus_tag="OO315_002489"
     CDS             1820..2566
                     /gene="mipA"
                     /locus_tag="OO315_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5891358.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2656..3417
                     /locus_tag="OO315_002490"
     CDS             2656..3417
                     /locus_tag="OO315_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5891359.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3468..4352)
                     /locus_tag="OO315_002491"
     CDS             complement(3468..4352)
                     /locus_tag="OO315_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5891360.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4436..5431)
                     /gene="gapA"
                     /locus_tag="OO315_002492"
     CDS             complement(4436..5431)
                     /gene="gapA"
                     /locus_tag="OO315_002492"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5891361.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5773..6186
                     /gene="msrB"
                     /locus_tag="OO315_002493"
     CDS             5773..6186
                     /gene="msrB"
                     /locus_tag="OO315_002493"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5891362.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6228..6500
                     /locus_tag="OO315_002494"
     CDS             6228..6500
                     /locus_tag="OO315_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5891363.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6870..7946
                     /locus_tag="OO315_002495"
     CDS             6870..7946
                     /locus_tag="OO315_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5891364.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7973..9352
                     /locus_tag="OO315_002496"
     CDS             7973..9352
                     /locus_tag="OO315_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5891365.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9369..10414
                     /locus_tag="OO315_002497"
                     /pseudo
     CDS             9369..10414
                     /locus_tag="OO315_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10435..11271
                     /locus_tag="OO315_002498"
     CDS             10435..11271
                     /locus_tag="OO315_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5891366.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11276..12223
                     /locus_tag="OO315_002499"
     CDS             11276..12223
                     /locus_tag="OO315_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5891367.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12233..13213
                     /gene="ydjG"
                     /locus_tag="OO315_002500"
     CDS             12233..13213
                     /gene="ydjG"
                     /locus_tag="OO315_002500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5891368.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13350..14108
                     /locus_tag="OO315_002501"
     CDS             13350..14108
                     /locus_tag="OO315_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5891369.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14226..15584
                     /locus_tag="OO315_002502"
     CDS             14226..15584
                     /locus_tag="OO315_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5891370.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15677..16318)
                     /gene="pncA"
                     /locus_tag="OO315_002503"
     CDS             complement(15677..16318)
                     /gene="pncA"
                     /locus_tag="OO315_002503"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5891371.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16329..17345)
                     /gene="ansA"
                     /locus_tag="OO315_002504"
     CDS             complement(16329..17345)
                     /gene="ansA"
                     /locus_tag="OO315_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5891372.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17512..19368)
                     /gene="sppA"
                     /locus_tag="OO315_002505"
     CDS             complement(17512..19368)
                     /gene="sppA"
                     /locus_tag="OO315_002505"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5891373.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19529..20080
                     /locus_tag="OO315_002506"
     CDS             19529..20080
                     /locus_tag="OO315_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5891374.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20197..21240
                     /gene="selD"
                     /locus_tag="OO315_002507"
     CDS             20197..21240
                     /gene="selD"
                     /locus_tag="OO315_002507"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5891375.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21245..23206
                     /gene="topB"
                     /locus_tag="OO315_002508"
     CDS             21245..23206
                     /gene="topB"
                     /locus_tag="OO315_002508"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5891376.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23333..24373
                     /locus_tag="OO315_002509"
     CDS             23333..24373
                     /locus_tag="OO315_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5891377.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24490..>25685)
                     /gene="gdhA"
                     /locus_tag="OO315_002510"
     CDS             complement(24490..>25685)
                     /gene="gdhA"
                     /locus_tag="OO315_002510"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5891378.1"
                     /translation="LERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGG
                     MRFHPSVNLSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGKVMRFCQTLMT
                     ELYRHLGADTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEA
                     TGYGLVYFTEAMLKRHGMGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTV
                     VDESGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNE
                     LDVDAAHQLIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEM
                     AQNAARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAML
                     AQGVI"
BASE COUNT         6522 a         5981 c         6628 g         6554 t
ORIGIN      
        1 tccgctattg ccagtacacc tcaatctgct gttccaggac gtagaacaga tggaccgggt
       61 ttgccgccac ttctacatga tcgaagttga acacgcggga gaggatctta aacgcaaaac
      121 gcgtcgacag accgttcatc ccttcatcga caccggcgta gtcacgatat tcctgatacg
      181 acttggcttt cggatcggtg tctttcagac tttcgccatc ataaacccgc atctttgaat
      241 aaatgctgga gttttctggc tctttcaggc gcgaaagaat ggaaaaacgt gacagtgttt
      301 cgagcgtgcc aggggcgcat ggggcgtgag tcaattcact gtgattaagc aatttctcgt
      361 agattttgat ctcttcggaa atgcgcaagc aatacggcac cttcacgatg taaacacggt
      421 cgaggaaggc ttcgttgttt ttgttattac ggaaagtgac ccattcggac ttgttcgagt
      481 gggcgagaat aatcccgttg aacggcaggg cggagatacc ttccgtcccg ttgtagttac
      541 cttcctgagt ggcggttaac aagggatgca gcacttttat cggtgcttta aacatctcaa
      601 cgaattccat gatcccctga ttggcgcggc acagcgcacc ggaatagccg taggcgtccg
      661 ggtcattctg cgcgtagtgt tcgagtttac gaatatcgac tttcccaacc agcgcggaga
      721 tgtcctggtt gttctcatca ccgggttccg ttttggcgat agcaatttgt tgcagaattg
      781 acggccagac cttcactacc cggaacttag tgatatcgcc accaaattca tgcaggcgtt
      841 ttgccgccca tggcgacatg atggtgccga gataacggcg aggaatgcca tactcttttt
      901 ccagaatctg agcatcttcc tgcggattga aaagacagaa cggatgatcg ttgaccgggc
      961 tacgctcacc gttggcgctc aatacataaa tcggtacgag ctgcattaat gatttcagtc
     1021 gctcagcaag cgatgattta cccccaccca caggccccag cagatacagg atttgtttct
     1081 tctcttccag cccctgagcc gcgtgtttca gataagagac aatctgttca atcgcgtctt
     1141 ccatgccgta aaactcttca aatgccggat aacgtgcaat gacccggtta gaaaagagtc
     1201 gagaaagtct ggggtcctgg gctgtatcga ccatgacagg ctcaccgata gccatcaata
     1261 gccgctcagc cgcgttggca taagcactgc gatcttgccg acaagtggta agaaactcct
     1321 gcagtgtgaa ctcttcgtcc ttggcagctt catatcgctg gcgatagtga tcgaatatat
     1381 tcatggtatg ccgtcctttc gttttttagc acaggttaag agccgttcat atgaatagta
     1441 gaggctcccg gaagaggtaa actgaacgac gtctcttgct aaaacacagc aactctcatg
     1501 ccattttatc gacgatgaga acaacgtgcc cggtgataca ccttcaaaat taagcgtaga
     1561 tggcaaatgg aaaacttgcc tgcacggctt tcttaatttc aatggcatat caacaactca
     1621 tccataaatt ttgcataatt aatgtaaaga ccaggctcgc cagtaacgct aaatccattt
     1681 ggctgtaagc gcggtgtcat ccgcggcagg aaaattaaac agttacttta aaaaatgaaa
     1741 acgtaaaaag gttgggtttc gatgtattga cgggtaaact ttgtcgcccg ctaaacattt
     1801 gtttaaggaa tgattaattg tgaccaaact caaacttctg gcacttggag tgcttatcgc
     1861 aacgtctgca ggcgtagcgc acgctgaagg taaattttcc ctgggcgcag gcgtaggtgt
     1921 cgttgagcac ccatataaag attacgatac cgatgtttac ccagtaccgg taatcaacta
     1981 tgaaggcgat aacttctggt tccgtggctt aggtggtggt tactacctgt ggaatgacgc
     2041 aacggataaa ctttcaatta ccgcttactg gtcgccgctt tacttcaaag cgaaagacag
     2101 tggcgatcac caaatgcgtc acctggatga ccgtaagagc accatgatgg ctggtctgtc
     2161 ttatgctcac tttacccagt acggttacct gcgtaccacc ctggctggcg ataccctgga
     2221 taacagcaac ggcattgtct gggatatggc ctggttgtat cgttacacca acggtggcct
     2281 gaccgtgact ccgggtattg gtgtgcagtg gaacagcgaa aaccagaacg aatactatta
     2341 tggcgtatcg cgcaaagagt ccgctcgcag cggtctgcgt ggctataacc cgaatgacag
     2401 ctggagccct taccttgagc tgagcgccag ctacaacttc ctcggcgact ggagtgttta
     2461 cggtaccgcg cgctacaccc gtctgtctga tgaagttact gacagcccga tggtggataa
     2521 atcctggact ggcctgattt ctaccgggat cacctacaaa ttctgataat gcatctttcc
     2581 agggcatttt gtgcataaaa acagggcgtc acgccctgtt ttgcattacg gtggtgcaat
     2641 acctgggagg aaacaatgca acaaaaaatg attcaattta gtggcgatgt ctcactgcca
     2701 gccatagggc agggaacatg gtatatgggc gaagatgcca gtcagcgcaa aacagaagtt
     2761 gctgcactac gcgcaggcat tgaactcggt ttaaccctca ttgataccgc cgaaatgtat
     2821 gccgatggcg gtgccgaaaa ggtggttggg gaagcattaa ccggtctgcg agagaaggtc
     2881 tttctcgtct ctaaagtcta tccgtggaat gctggcgggc aaaaagcgat aaatgcatgc
     2941 gaagccagtt tacgccgtct caatactgac tatctcgatc tctacttatt acactggtct
     3001 ggcagtttcg cttttgaaga gactgttgct gcgatggaaa aattgatcgc ccagggaaaa
     3061 atccgtcgct ggggcgtttc taaccttggt tatgccgata tgcaggaact ctggcagctg
     3121 ccggggggaa atcagtgtgc cactaatcag gtgctttacc atctcggttc acgagggatt
     3181 gagtacgatc tactcccctg gtgccagcaa cagcagatgc cggtgatggc ttacagtcca
     3241 ttagcccagg ccgggcggtt gcgcaatgga ctgttaaaaa acgcggtagt caacgaaatt
     3301 gcacatgtcc aacaacatgc agctgcgctt gaggtcgaac tttcgtcagc ggaattagct
     3361 atgctggata aggcatatcc ggcaccaaaa ggaaaaactg cgctggatat ggtgtgatgt
     3421 tgatacgtgc cggatgcatc gcacccggca cacaacatta acgcaaatta acgtttggca
     3481 acgcgaatgg attgcgccag atgtgcaggt ttctctttgg tgactttttg cgtttctgaa
     3541 gcgtaagccg tttctacaca aacaaaggtc ttgtagccat cgtccggcat gtcgcccatg
     3601 ctaactgaaa gcgccggtcc cgggttccag ccgacaacgt tcagatgatg ctggtggcct
     3661 acggcgataa tacgattcag cgcttcatca ttaatcacgc tgcaatcttg cggattcaga
     3721 tacacgcgat cggtacggtc agggaaggtc tgaataccgt cggtcagtac atcttctttc
     3781 gcgtcattca ctttatcaat gaagcgatcg cccagcccac tgacgcttac cttagcgata
     3841 tcacccacgt taaagtaggt atgcagggca gaggtggttt caaattcgcc atgtgattca
     3901 agatcgattt cacaggtttt acccacgcgg aaatgcgcta acagcgtaaa gtcgtgcggc
     3961 cagaattttt tcgtctcttc gctttgtgtc aattcaaaag tcagcgctac gccatcagca
     4021 tcttcatgat gtgatttcag cgtccacggc aggttgcggg caaaaccgtg cgcaggcaga
     4081 ccttgttgtg ccgccggacc aaaccacggc cagcaaaccg gtacgccacc gcgaatagcg
     4141 acgccatttt tgaacggtgt gttgttgctc aaccacagaa cttcttcttc acccgcaggt
     4201 ttccacgaga gaaggtgtgc gccctgtaat gcaaaagagg cttttacctg gggatgatcg
     4261 accacaatga ggtccagttc atccagttta cgacgggaga ggacagggga gatttgttcg
     4321 atgaccggaa gggcaaaaat tttcttaatc atgacgcagt cctttaactt cattttatca
     4381 ggtaaaaaaa agagcgaccg aagtcgctct ttttagatca cagtgtcatc tcaacttatt
     4441 tggagatgtg agcgatcagg tccagaactt tgttggagta accggtttcg ttgtcgtacc
     4501 aggataccag tttcacgaag ttgtcgttca gagcgatacc agctttagca tcgaacacgg
     4561 aagtgcaaac ttcgccgttg aaatcggtag atactacgtc atcttcggtg tagcccagaa
     4621 cgcctttcat ttcgccttca gcagcagctt taacggcagc tttgatctgc tcgtaagttg
     4681 cagctttttc cagacgaacg gtcaggtcaa ctacggatac gttcggggtc ggaacgcgga
     4741 acgccatacc agtcagtttg ccattcagtt ctggcagtac tttacctaca gctttagcag
     4801 caccggtaga ggacgggatg atgttctggg aagcgccgcg gccgccgcgc cagtctttgt
     4861 gagacgggcc atcaacggtt ttctgagtag cggtagtagc gtgaacggtg gtcatcagac
     4921 cttcgatgat gccgaagtta tcgttgataa ctttagccag cggagccagg cagttggtgg
     4981 tgcaggaagc gttggaaacg atgtcctggc cagcatattt gtcgaagtta gcgcctttaa
     5041 cgaacatcgg agtgttgtct ttagacggac cagtcataac cactttcttc gcaccagcgg
     5101 tgatgtgttt acgagcagtt tcgtcagtca ggaacagacc agttgcttca gcgacaacgt
     5161 caacacccac ttcgtcccat ttcaggttag ccggatcacg ttcagcggta acacggattt
     5221 ttttaccgtt aacgatcaga tgaccgtctt tcacttcaac ggtaccgtcg aaacggccgt
     5281 gagtggagtc atatttcagc atgtatgcca tgtaatcagc gtctaacagg tcgttgattg
     5341 caacgatctc gatgtcagaa cgtttctgag cagcacggaa aacaatgcga ccgatacggc
     5401 caaaaccgtt gatacctact ttgatagtca tatattccac cagctatttg ttagtgaata
     5461 aaaggttgcc tgtaaaatta caaaaacctt acgcagcgtc aagcggaatc gtgtcaatca
     5521 ttgcgacaaa tcaatcctgt gcctaagcat tacgcgactg actcgcctca cttttccttt
     5581 gggcttgaga ccacatgggg tcggcgcccc gaattttaaa gggcaattac gataaaaatg
     5641 tgattttcat cacgatttag gtgcagccat ttcgtcgaga tcaagtttag gacaaaattt
     5701 tgccagttgg tgttaatgtt ttgttagaat cagtcacgta atgtgagcac gattaaagtg
     5761 agatgtgagt aaatggcgaa taaaccttcg gcagaagaac tgaaaaaaaa tttgtccgag
     5821 atgcagtttt acgtgacgca gaatcatggg acagaaccgc catttacggg tcgtttactg
     5881 cataacaagc gtgacggcgt atatcactgt ttgatctgcg atgccccgct gtttcattcc
     5941 gaaaccaagt atgattccgg ctgtggctgg cccagtttct acgaaccggt gagtgaagaa
     6001 tccattcgtt atatcaaaga cttgtcacat ggaatgcagc gcatagaaat tcgttgcggt
     6061 aactgtgatg cccatctggg gcatgtcttt cccgacgggc cgcaaccaac aggcgaacgt
     6121 tattgtgtta actctgcttc tttacgcttc actgatggcg aaaacggcga agaaatcaac
     6181 ggttgaaaaa acgattcagc aacttattcc acaggagcgg aaaaattatg aatcttgatg
     6241 acattatcaa cagcatgacg cctgaggtat accagcgttt gtcgaccgcc gttgaactgg
     6301 ggaaatggcc tgatggcgtt gcgttgaccg aggaacaaaa agagaattgc ctgcaactgg
     6361 tgatgctgtg gcaagcccgc cataataccg aagcacagca tatgacaatt gacacaaatg
     6421 gtcagatggt catgaagagc aaacagcagt taaaagaaga ttttggtatc tcagcaaagc
     6481 ctattgccat gtttaagtaa gtggttcatc ccctttttgc cgcggctatg aaggggatag
     6541 ccaacaatga ccccacgact cgtctcaatt atctcgaccc gacctggcta agtcccagtc
     6601 cgatctcttg cagtcgcgtt cacgcctgaa tgacttctcc tgaacttaag tctcagacct
     6661 atttggccgg taatccctct cgaatttgct ctttttgcgt cccgccttcc accaacctgt
     6721 aacagaccca gccattttct gcgtaattgt gatttgagtt gtaaaatgca ggtcattatc
     6781 tgtgttgtgc agatttaata ttgacctcaa aaatcatgtg ggtaagtatg atggccatct
     6841 aagcaaaaca aaacacagag gatgacgcaa tgaaagcact ggctcggttt ggcaaggcct
     6901 ttggcggcta caagatgatt catgtcccac aacccatttg tggcccggaa gacgtagtga
     6961 ctgaaattaa agccgcggca atctgcggcg cagacatgaa gcactacaat gtcgatagcg
     7021 gttctgatga gtttaactct atccgcggcc atgagttcgc aggttgtatt gcgcaggttg
     7081 gtgaaaaagt caaagactgg aaagtggggc aacgcgtcgt gtcggataac agcggtcacg
     7141 tttgcggcgt ttgtccggcc tgtgaacagg gtgattttct gtgttgtaca gaaaaagtaa
     7201 accttggtct ggataacaat acctggggcg gtggtttttc caaatattgt ctggttcctg
     7261 gtgaaattct caaaattcat cgtcatgcgt tgtgggaaat ccctgatggt gttgattatg
     7321 aggacgcagc cgtacttgac cctatctgta atgcctataa atccatcgcg cagcaatcga
     7381 aattccttcc tggtcaggat gttgtcgtca ttggcactgg cccactcggg ctgttttccg
     7441 tacaaatggc gcggattatg ggggcggtaa atatcgtcgt cgttggtctg caagaagatg
     7501 tggcggtccg cttcccggtt gcaaaagaac tgggggcgac ggcagtagta aatggttcta
     7561 ccgaagatgt ggtggcgcgc tgccagcaaa tttgtggcaa agataatctg ggactggtga
     7621 ttgaatgctc cggtgccaat atcgcactga aacaagccat cgaaatgctc cgcccgaatg
     7681 gggaagtggt acgcgttgga atgggcttca aacctcttga tttctcgatt aatgacatta
     7741 ccgcctggaa taaaagcatc attgggcata tggcctatga ctccacctca tggcgtaacg
     7801 ctatccggct attagccagc ggcgctatca aagtcaaacc gatgatcacg catcgtatcg
     7861 gcctgtcgca atggcgcgaa gggtttgatg cgatggtcga taaaaccgca atcaaagtga
     7921 tcatgactta cgactttgat gaataactac tctcctgaag gaacgttatc gcatggaaca
     7981 gataacaaaa ccgcattgtg gtgcacggct ggatcgctta ccggattgcc gctggcattc
     8041 gtcaatgttt gccatcgtcg cgtttggctt gcttgtctgc tggagtaatg ccgttggtgg
     8101 cttgatcctc gcgcagctga aagcgttggg ctggacagat aattccacta cagccacatt
     8161 ctcagcaatc acgaccgccg gaatgtttct cggtgcgctg gtgggcggca tcattggcga
     8221 caaaaccggt cgcagaaatg cgttcatcct ctatgaggcc attcatattg cctcgatggt
     8281 tgtcggtgct ttctcaccga atatggattt tcttattgcc tgccgttttg tgatggacgt
     8341 tgggctggga gctttactgg ttacactgtt tgctggtttc accgaatata tgcccggtag
     8401 aaatcgcgga acgtggtcca gtcgggtttc ctttattggc aactggtcat atccgctctg
     8461 ctcactgatt gcgatgggac tcacgccgct gattagtgca gagtggaact ggcgggtaca
     8521 actgcttatt cctgcaatat tgtcgcttat cgctacggcg ctggcctggc gctactttcc
     8581 tgaatccccg cgctggctgg aatcgcgcgg acggtatcag gaagccgaga aagtcatgcg
     8641 gagtatagaa gaaggcgtca tacgccagac gggtaaacct ttgccgcctg tggttattgc
     8701 tgatgacggt aaagcgccac aagcggtgcc gtattcagcc ttactgacag gagtattact
     8761 gaaacgcgtg atattaggct cttgtgtgct gattaccatg aacgttgtgc agtacacact
     8821 aattaactgg ttgccaacaa tattcatgac ccaggggatt aatttaaaag actcgattgt
     8881 tttaaatacc atgagtatgt ttggcgcgcc atttggtatt tttattgcca tgctggtgat
     8941 ggataaaatt ccgcgtaaaa caatgggtgt ggggctatta atcctgattg cggtgctcgg
     9001 atatatctat tcactgcaaa ccagtatgtt gctcataacg ctgattggtt tcttcctgat
     9061 tactttcgtc tatatgtacg tttgctatgc ctcggcagtg tatgtccctg aaatctggcc
     9121 gacagaggcc aaactccgtg gctccggtct ggcgaatgcg gtagggcgaa tcagtggtat
     9181 tgccgcacct tatgccgttg cagtgctgct cagtagttat ggtgtaacgg gagtctttat
     9241 tcttctgggg gctgtttcaa atattgtcgc aattgctatc gccaccattg gaattgaaac
     9301 caaaggtgtc tccgttgaaa gtttaagtat tgatgcagta gccaataaat aatacgggtg
     9361 gattcaaaat gaaaaattca aaagcaatat tgcaggtgcc gggcacaatg aaaattattt
     9421 cagcagaaat accagtgcct aaagaagatg aagttttgat taaagtagaa tatgtcggta
     9481 tttgtggttc agatgtacat ggttttgaat caggcccgtt tattccgcct aaagacccaa
     9541 atcaagaaat tggcctgggt catgaatgcg ccgggacggt tgtggctgtg ggaagccgcg
     9601 tgcgcaaatt taaaccgggg gatcgggtaa atatcgaacc tggcgttcct tgcggtcact
     9661 gtcgttactg tctggaaggc aaatataaca tctgcccgga cgttgatttt atggcgacac
     9721 aacccaacta ccgcggcgca ttaacgcact atctgtgtca tccggagagc tttacttaca
     9781 aactgcccga caatatggac acgatggaag gggcgctggt ggagcctgcc gcagtcggga
     9841 tgcatgccgc gatgctggca gatgttaaac cgggtaagaa gataattatt ctgggagcag
     9901 gttgtattgg tttgatgacg ttgcaagcgt gcaaatgcct gggcgcaacg gaaattgccg
     9961 tcgttgatgt actggaaaaa cgtctggcaa taggggaaca gcctggtgcg acagtggtta
    10021 ttaatggcgc aaaagaggac actattgcac gctgtcagca atttaccgaa gacatgggcg
    10081 cagatattgt tttcgaaaca gcgggttctg cggtcaccgt taaacaggca cctttaatct
    10141 ggtaatgcgc ggcggtaaaa ttatgattgt tggtactgta cccggcgatt cggcaatcaa
    10201 tttcctcaaa atcaatcgcg aagtcactat ccagacggta ttccgctatg ccaatcgtta
    10261 tccggtcacg attgaagcta tttcttcagg gcgattcgat gtgaaatcga tggtgacgca
    10321 tatttacgat tatcgggatg tacaacaggc atttgaagag tcagttaaca acaaacgcga
    10381 cattattaaa ggcgttatta aaattagcga ttaattaata cgggagaaca gaacatgctc
    10441 gcagatatca gatattggga aaacgatgcc acaaataaat attatgcaat tgcccatttt
    10501 aacgtatgga atgcagaaat gttgatgggc gttaaagacg cagccgaaga agcgaaatcc
    10561 ccggttatta tttcttttag tacaggtttt gtcggtaaca cctcatttga agatttctct
    10621 cacatgatgg tatcaatggc acaaaaagca acggtgccgg taataactca ttgggatcat
    10681 ggtcggagta ttgagattat tcataacgcc tggactcatg gtatgaattc attaatgcgt
    10741 gatgcttctg catttgattt cgaagaaaat attcgtttaa ccaaagaggc tgttgatttc
    10801 tttcatccgc tgggtattcc ggtagaggcg gaattagggc atgtcggtaa tgaaaccgtt
    10861 tatgaggaag cgctggcggg ctatcactat accgatcctg accaggcggc tgaatttgtt
    10921 gaacgtacgg gctgtgactc attggctgtc gccatcggca accagcatgg ggtttatacg
    10981 tcagagccgc aattgaactt tgaggtcgtc aaacgcgtac gcgatgctgt ttctgttccg
    11041 ctggttttgc atggcgcatc ggggatcagt gatgccgaca ttaaaactgc aatttcgttg
    11101 ggtattgcga aaatcaacat tcatacggag ctctgtcagg ccgcaatggt agcagtgaaa
    11161 gaaaaccagg atcagccttt cctgcattta gaacgtgaag tacgtaaagc tgtaaaagaa
    11221 cgggcattgg aaaaaattga actgttcggt tctgatggca aagcggaata atgatatgga
    11281 taatctcgac gttatttgta taggtgccgc tattgttgat attccattac aaccggtcag
    11341 taaaaatatc tttgatgtag attcttatcc tcttgaaaga atcgcaatga gcaccggggg
    11401 cgatgcaata aatgaagcaa caattatttc tcgtctgggg catcgtaccg cattaatgag
    11461 ccgcattggt aaagatgcct cagggcaatt tattctcgac cattgtcgca aagagaatat
    11521 tgatattcaa agcctgaaac aggacgtcag catagataca tctattaacg ttggactggt
    11581 gacggaggat ggcgagcgga cgtttgtcac gaaccgtaac ggcagtttgt ggaaattaaa
    11641 tattgacgat gttgattttg cgcggtttcc tcaggcgaaa ttattatcac tggccagtat
    11701 tttcaacagt cctctattgg atggtaaagc gctaacagaa atttttacgc aagcgaaagc
    11761 ccggcagatg atcatttgtg ccgatatgat caaaccgcgg ttgaatgaaa cgctggatga
    11821 tatttgcgaa gcattgagtt atgtcgatta tctgtttcca aattttgccg aggaaaaatt
    11881 actcacaggg aaagagacac tggatgaaat tgctgactgc tttcttgcgt gcggcgtaaa
    11941 aacggtggtg attaaaacgg gtaaagacgg ctgctttatc aagcgtggtg acatgacgat
    12001 gaaggtgccg gcggtcgcag gaataaccgc catcgacacc attggcgcgg gcgataactt
    12061 tgcttcaggt tttattgcgg cactgttaga aggcaaaaat ctgcgtgaat gcgcacgctt
    12121 tgccaatgca acggcggcta tctcggttct aagcgtcggt gccaccaccg gcgtaaaaaa
    12181 cagaaagctg gtcgaacaat tgctggaaga atacgaagga taatgaaggc aaatgaaaaa
    12241 gataccttta ggcacgacgg atataacgct ttcgcgaatg gggttgggga catgggccat
    12301 tggcggcggt cctgcatgga atggcgatct cgatcggcaa atatgtattg atacgattct
    12361 tgaagcccat cgttgtggca ttaatctgat tgatactgcg ctaggatata actttggcaa
    12421 tagtgaagtt atcgtcggtc aggcgttaaa aaaactgccc cgtgaacagg ttgtagtaga
    12481 aaccaaatgc ggcattgtct gggaacgaaa aggaagttta ttcaacaaag ttggcgatcg
    12541 gcagttgtat aaaaaccttt ccccggaatc tatccgcgaa gaggtagaag ccagcttgca
    12601 acgtctgggt attgattaca tcgatatcta tatgacgcac tggcagtcgg tgccgccatt
    12661 ttttacgccg atagctgaaa ctgtcgcagt gcttaatgag ttaaaggctg aagggaaaat
    12721 tcgcgctata ggcgctgcta acgtcgatgc tgaccatatc cgcgagtatc tgcaatatgg
    12781 tgaactggat attattcagg cgaaatacag tattctcgac cgggcaatgg aaaacgaact
    12841 gctgccgcta tgtcgtgata atggcattgt ggttcaggtt tattccccgc tagagcaggg
    12901 attgttgacc ggcactatca ctcgtgatta cgttccgggc ggcgctcggg caaataaagt
    12961 ctggttccag cgtgaaaaca tgctgaaagt gattgatatg cttgaacagt ggcagccact
    13021 ttgtgctcgt tatcagtgca caattcccac tctggcactg gcgtggatat taaaacagag
    13081 tgatttaatc tccattctta gtggggctac tgcaccggaa caggtacgcg aaaatgtcgc
    13141 ggcactgaat atcaacttat cggatgcaga cgcaacattg atgagggaaa tggcagaggc
    13201 cctggagcgt taaatactca acacgattca cctctgtttc tgtgaaaact gtgatgaaaa
    13261 tctgtgattc aaataggtta ttttgaaagt aaacatcggt tatgcgataa tcgcgctaat
    13321 gtgatgtaaa aagatacagg ggtgtaatcg tggcggcaaa agacaggatt caggcaatta
    13381 agcaaatggt tgccaacgat aaaaaggtga cagtctcaaa tttgagtggg atttttcagg
    13441 taaccgaaga aaccattcgc cgcgatcttg agaagctgga agatgaaggc tttttgacca
    13501 gaacctatgg tggtgctgtt ttaaatacag cggtgttgac ggagaatatc catttttata
    13561 agcgcgcttc atcgttttat gaagagaagc tgcttattgc acgcaaggca ctacccttta
    13621 tcgacaataa aaccaccatg gcagccgatt ccagtagtac cgtaatggaa ttgctcaaat
    13681 tgttacagga ccgtagtgac ctgacgttgc taaccaactc ggcggaagcg attcatgttc
    13741 tggctcagtc agaaattaaa gtcgtttcaa cgggtgggga actaaacaaa aatacacttt
    13801 ccctgcaagg aagaattact aaagagatca tcagccgcta ccatgtcgat atcatggtaa
    13861 tgagttgtaa aggtcttgat attaacagcg gcgcgctgga ctctaatgaa gcggaagctg
    13921 aaatcaaaaa gacaatgatc cgtcaggcga cagaagttgc gttattggtt gatcactcta
    13981 agtttgatcg taaagctttt gtccagttag ctgactttag tcatattaat tatataataa
    14041 ctgataaatc accgggtgca gagtggatag cattttgcaa agacaataat atccagctgg
    14101 tgtggtaatt taagcacacg ttgtcaatat tatttgttcc tgcgactgga aataaaatga
    14161 tgataaagat gcggcaagtg aatttgccgc aattgctacc ttttatagca aatccgaggc
    14221 acccaatgga acaatatgat caaattggcg caagactgga ccgcttgcct ttggcccggt
    14281 ttcattatcg tatatttggt ataataagct ttagtctgtt attaacgggg tttttgagtt
    14341 actcaggtaa tgtcgtctta gcaaagctgg taagcaatgg atggtcaaat aatttcctct
    14401 atgccgcctt tacctcggca ttaatgtttg gttatttcat cggctcactt actggtgggt
    14461 ttattggtga ctactttggg cggcgcaggg cgtttcgcat aaatcttctc atcgtcggta
    14521 ttgctgcaac aggggccgct tttgtccctg atatgtactg gctcattttc tttcgcttcc
    14581 tgatgggaac aggaatgggg gcgctgatta tggttggcta tgcctcattt acggagttta
    14641 tccccgcgac ggtgcgtgga aaatggtccg cgcggctctc atttgttggt aactggtcgc
    14701 ccatgctgtc tgcggcgata ggcgtggtgg ttatcgcttt ttttagttgg cgaataatgt
    14761 ttcttctggg tggtattggc atactgttag cctggtttct ctcaggtaaa tactttatcg
    14821 agtcgccacg atggctggaa gggaaagggc aaatcgccgg tgcagaaagc caacttcgtg
    14881 aagtagagca gcaaattgaa agggagaaga gtattcgttt accccagctt actttgaacc
    14941 agagcaacag caaggttaag gtaatcaagg gtaccttcag gctcctgttt aaaggggaaa
    15001 tgttacgacg tacattagtt gcgataactg ttttaattgc aatgaacatt tcgctttata
    15061 ccattaccgt atggataccg accatatttg ttaactccgg cattgatgtc gataaatcaa
    15121 tattaatgac cgctgttatt atgattggtg ctccggtagg aatatttatt gcggcattaa
    15181 ttattgatca ttttcctcgt cgattatttg gctccgcctt acttattatt attgccgtgt
    15241 taggctatat ctattcaagt cagactacag agtgggcgat tttaatctat ggtctggtga
    15301 tgatcttctt tttatacatg tatgtttgct tcgcgtcggc ggtttatatc ccggagcttt
    15361 ggccaacgca tttacgcctg cgcggttcgg gtttcgttaa tgccgtcgga cggatcgtcg
    15421 cagttttcac gccctatggc gttgcggcat tattaacaca ttatgggtcg atcacggtgt
    15481 ttatggtgct tggtgtcatg ttagtgctct gtgcgctggt tctctccatt tttggcatcg
    15541 aaacgcggaa ggtgtcgctg gaagagattt ctgaggtgaa ttaaatcttt cgttgatggc
    15601 atcaattgcc agatgtacca ctgacgtgtt ttatttggcc tacaatgcca gtacagaatt
    15661 gtaggccagc gtaaaattac ccctgcgtct cttcccagtc tgccagcgta tatagcgttg
    15721 ccccagctgc tgacatctcc ataaacgcgt gtgcactgtc ctggggctgg atattcacgc
    15781 cacgacagcc atcggtaatc acgtttacct tataacctaa ctgtaacgcg tccagcacgg
    15841 taaacttcac gcaatagtca gtagccaggc ccataacgat caattcatcg atttcatgat
    15901 cgcgcaacca gtcatcaagc gcggttttct gccgacgtcc gttatcaaaa aaggcactgt
    15961 aactgtcaac taaaggattt tcgcctttat ggaacaccgc tgcgatctct ttttgattca
    16021 gtaacggatg taattgtgcg ccttcactgt tctgcacaca gtgttcaggc cagaaagttt
    16081 gccgcaggcc gccgagttgg cctggcgtat aaggctctac gccgtgctgg ctggcaaagc
    16141 tgccgtgatt cgccggatgc cagtcctggc tggcgataac cgcttctccg cgcgactggc
    16201 accagtcaat aaggcggtta gcaacctcca ccgtactgtc accttccggt acggcgagcg
    16261 cgccaccagc acagaaatca ttttgtaaat cgaccagtaa cagggcgcga tggggcattg
    16321 cagtctcctt aatcatccgg cgtcagttcg ccgcgcaggt tttggctcat ggccttgcga
    16381 atggtttcag tatccagttc ctggctcagc aggtaatgca gtttggttag cgtggcttct
    16441 acagtcatat ctgcaccgtc aataacgccg gcatgggcga gggcgttacc ggtggcataa
    16501 ccactcatat tcactttacc ggacatacat tgtgtcaggt tgaccaccac aataccgcga
    16561 tcgctggctt cttgtaattc ctgcaggaag gctttgtttt gtggcgcatt acccacgcca
    16621 taggagcgca gaatcaatgc tttcaccggt tggcgcagaa aattgcgcac gacgtcagca
    16681 gaaatccctg gataaatcgt caccacgcca attggttgtg gcgtgattgg atgaacgatt
    16741 aattcccctt caccgtgcgg ggcgggtggc gtattcaaac gacgaatatg gataccagct
    16801 tccagtaacg gaggaaggtt tggagaggca aacgcatcaa aaccatcggc atgggctttg
    16861 gtagtgcggt tgccgcgata caatcggtta ttgaaaaaga gcgttacttc gttgatcgga
    16921 taattcggcg caacgtacaa cgcattcagc agattaattt gtccgtcaga gcgtaactca
    16981 gccagcggga tttgtgaccc tgtcacaatg accggtttac cgagattctc gagcatgaac
    17041 gacagcgcag aggcggtata cgccatcgtg tcggtaccgt gtagaatgac aaaaccatca
    17101 tagtcgtcat agtgcgcttt aatatcttca gcaatatgct gccagtcttc cggcgtcata
    17161 tcagaagaat ccatcaacgg cgtatattca tgaatggtga aatctggcat ctccgggcga
    17221 tggaattccg gcatcagcgc cagttggcgt tgtagatgac ctgacaccgg aatataaccc
    17281 tgctcggaac gctgcatccc gatggtcccg cccgtgtagg cgacgtaaat tgatttcttt
    17341 tgcatgatat tgatatgaat gatatcgaaa gagcaaaagt atatacgtga ggcaggagga
    17401 gcgcacctga tactatgcgg ataaggaaat atggggcgag acagcgggaa gaggattcgt
    17461 cgaggtaaaa aataatgtgg ccgacgatct gtcggccact cagtacaaga cttaacgcac
    17521 gttggcgcag gtcaggcaaa acgcataacg gttttgtggg tcgttaaacg cggccagctt
    17581 gtcgctttca cttttaacgg tagaggctac cgaggcaagc ggtgcaggta acatggcctg
    17641 gaacgcatct ggcaacattg cccggacaga accagacatg ttgtccatca ctttgtcgaa
    17701 gaaggtcggt tcatcaacgt agtattccag atgccactgt ttcacttttg cgagctctgc
    17761 tgctttggca accgcatcat cgaaatcccc gagactatcg accagcccgt tagcttttgc
    17821 atcctgaccg gtccagacgt ggccctgggc gattttatca atttgctccg gcgtcgaatg
    17881 acgcgcatca gcaaccagcg tgataaagcg tttatagcca ttctcaatgc ttaattgcat
    17941 catctgctgc gcttccggcg gcagtgccct ggtgatagaa acatccgcca gcggtgaagt
    18001 tgagacacca tcagtatgaa caccaatcga atccagacta ttttctacgg tggtgatcac
    18061 gccgaagata ccgatagaac cggtcagggt gctggggtta gccacaatgt aattagctgg
    18121 cgtggaaatc cagtaaccac cagatgccgc catgccgccc atcgatacaa ccacagactt
    18181 acccgctgcc cgggctgctg ccagttcagc gcgaatcact tcagacgcgg taacgctgcc
    18241 gcctgggcta ttaacacgca ggacaatcgc tttcactttc gggtcaaggc gagcgtcgcg
    18301 gatttgtgcc gcagtggtat caccgccaac attcccctga gtttcctcgc catccataat
    18361 tgcgccatta gcgaagacga caccgatgct gtcaccggta tctgccggcg ttttcaatgc
    18421 gtaatcgtaa taactgatgg cgcgataatt tttatcagtc ttactccagc cgaactcttt
    18481 ggtcagagct ttttcgattt cggcactcga tgccagtgca tcgaccagct tgttttccag
    18541 tgcatatttc gcggtatcgc caccggtttt ggttaaaccc tcaagcaacc cttgcgcgcc
    18601 agggaatacc tgctgagcag ggatctgccg gttagcggca acagtattca gatagttttg
    18661 ccacagctca ccaatccagc ggctgtcagc ttcgcgggct gccggtgaca tatcatcacg
    18721 aataaacggt tcaacggcag atttatacgt acccacgcgg aacacatggg tggaaacttt
    18781 cagcttatcc agcaacgatt tgtagtacag accgttggta gcaaaaccgt gcagatcaac
    18841 cacgccttgc ggtgacagcc aaattttatt ggcgaaactg gcgagataat attgcccctg
    18901 gctgtagttc tcgccaacgg cataaaccgg tttcccgctg tcacgaaact ctttcagggc
    18961 tttgccgatg tactgcatag acggctggtc gccgcctgcg aagtttttca gatccatcac
    19021 gataccggtg atattgcggt cgtccttcgc ctggcgaatg gtgttgacga tatcaaacag
    19081 tgagttttcc tgcagacgat cggaactggc accaagcagc tggcggctta atttactaaa
    19141 ccgctgagaa ctgtctggtt tatcgacgat cacaccagaa atgtccagca gcagtgcgcc
    19201 acgactggcc gtttctttcg aatcaccacc actgacctgc atccagatcc ccacaccaac
    19261 cagtacgagg aaaataaaga acaggttaag taccatttca cggacgaaat tcagcagacg
    19321 ccacgtccat ttaaaaaatc cggcaataaa tcgccaaagg gttcgcatgt attctcccaa
    19381 cttaaggtca cacctgtcac aggcgcaata gatgtggtta tcgtaatgac ccaaccggca
    19441 cttgtcagca ggaattgcgg actgtgctgt aacaaaatcc actctcgtgt taattttgtg
    19501 aataaatatc acgacaggag ttaatcaaat ggatgcactc gaactattga tcaatcgccg
    19561 tagcgcctcc cgcctggctg aacccgcgcc aacgggtgaa caactgcaaa acatcctgcg
    19621 tgcgggtatg cgtgcgccgg accataagtc catgcaaccg tggcattttt ttgtgattga
    19681 aggggaaggg tgcgagcgtt tcagcgccgt actggaacag ggggcgattg ctgccggtag
    19741 tgatgataaa gctatcgaca aagcccgtaa tgcgccgttc cgcgcaccgc tcatcatcac
    19801 ggtggtggcg aaatgcaaag aaaatcataa agtcccgcga tgggaacagg aaatgtctgc
    19861 cggatgcgcg gtcatggcga tgcaaatggc agccgttgcc cagggatttg gcggcatctg
    19921 gcgcagtggc gcattaactg aaagtccggt agtgcgtgaa gcattcggtt gccgtgagca
    19981 ggataaaatt gtcggttttc tctacctcgg cacgccgcag ttgaaagcat ctacgtcgat
    20041 taacgtcccg gacccgacgc cgtttgtaac ttatttctga taattgtcgc gaaactgtct
    20101 ggattgtgag caaaggcgca ggaattcaga cactctcact tatcacttca cggaatgagg
    20161 gttaccatac ccggattgca ttgacaggag atgtccatga gcgagaactc gattcgtttg
    20221 acccaataca gccacggagc tggttgcggc tgtaaaattt ccccaaaagt gttggaaacc
    20281 atcctgcaca gtgagcaggc gaagtttgtt gatccgaatt tgcttgtggg taatgaaacc
    20341 cgcgacgatg cggcggtgta cgatctgggc aatggcacca gcgttatcag taccaccgac
    20401 ttctttatgc cgatcgttga taatcctttc gattttggcc gcattgcggc gactaacgcc
    20461 atcagcgata tcttcgcgat ggggggcaaa ccgattatgg cgattgcgat cctcggctgg
    20521 ccgattaaca aactttcccc ggaaattgcc cgcgaagtaa ccgaaggtgg acgctatgca
    20581 tgccgtcagg cgggtattgc gctggctggc ggtcactcca tcgatgcgcc ggagccgatt
    20641 tttggtctgg cggtaacggg gatcgtaccg accgagcggg tgaagaaaaa cagtaccgca
    20701 caagccggat gcaaactgtt cctgacgaaa ccgctgggga tcggcgttct taccacggct
    20761 gagaaaaaat cactgttgaa accagaacat cagggactgg cgacggaagt gatgtgccgg
    20821 atgaacatcg caggcgcgtc ctttgccaac atcgaaggcg taaaagcgat gaccgacgtt
    20881 acgggctttg gtctgctggg ccacttgagc gaaatgtgtc agggggctgg tgtgcaggca
    20941 cgcgtcgact atgaagcgat cccgaaactc cccggtgttg aagagtacat taagttgggc
    21001 gcagtacctg gcggcactga acgtaacttt gccagctacg gtcatctgat gggtgaaatg
    21061 ccgcgtgaag tgcgcgatct gctgtgcgat ccgcaaactt ctggcggttt gctgctggcg
    21121 gtcatgccgg aagcagaaaa tgaggttaaa actacagccg ccgagtttgg cattgaactg
    21181 acggcgattg gcgaactggt gccagcgcgc ggcggtcgtg ccatggttga gatccgttaa
    21241 tttaatgcgg ttgtttattg ccgaaaaacc gagtctggcg cgcgccattg ctgatgtcct
    21301 gcccaaaccg caccggaaag gcgatggctt tatcgagtgc ggtaatggtc aggtggtgac
    21361 ttggtgtatc ggtcacctgc ttgagcaggc gcagccagac gcctacgaca gccgctatgc
    21421 gcgctggaat cttgcggatt tgccgattgt cccggaaaag tggcaattac agccacgacc
    21481 ctccgtgacc aaacaactta acgtcatcaa acgcttcctg catgaagcca gcgaaatcgt
    21541 tcacgccggg gacccggatc gtgaagggca attgctggtg gatgaagtgc tggactatct
    21601 gcaactggca ccggaaaagc gccagcaggt gcagcgttgc ttgataaacg acctgaaccc
    21661 gcaggcggtt gagcgggcga tcgaccgtct tcgttccaac agtgagtttg tgccattgtg
    21721 cgtttctgcg ctggcgcgag cgcgtgccga ctggctgtac ggcatcaata tgacccgtgc
    21781 gtataccatt ctcggtcgca atgccggtta tcagggcgta ctttccgtgg gacgcgtgca
    21841 gacacccgtg ctcgggctgg tggtgcgccg cgatgaagag attgaaaact tcgtggcgaa
    21901 agacttcttt gaagtcaaag cacatatcgt gacacctgcc gatgagcggt ttaccgctat
    21961 ctggcaaccg agcgaagcgt gtgaaccgta ccaggatgaa gaagggcgct tgttacatcg
    22021 tccactggcg gagcatgtgg ttaaccgcat tagtggtcaa ccggctattg tcaccagcta
    22081 taacgataaa cgggaatcag aatccgcgcc gctgccgttt tcgctttcgg cgttgcagat
    22141 tgaagcggca aaacgcttcg gtctgagcgc gcagaacgtg cttgatatct gccagaagct
    22201 gtacgaaacg cacaagctaa tcacttatcc gcgttccgat tgtcgctatt tgccagaaga
    22261 acattttgcc ggacgccacg cggtgatgaa tgccatcagc gttcatgcac cggatctgtt
    22321 gccgcagcca gtggtagatc cagatatacg caaccgctgt tgggatgaca aaaaggtcga
    22381 tgcgcaccac gccatcatcc cgaccgcacg gagttctgcg atcaacctga cggagaacga
    22441 agcgaaggtc tataacctga ttgcccgtca atacctgatg cagttctgcc cggatgcggt
    22501 gttccgcaag tgtgttatcg aactggacat tgccaaaggc aaatttgtcg ctaaagcccg
    22561 ttttcttgct gaagcaggct ggcgcacgct gttaggcagc aaagagcgcg atgaagaaaa
    22621 cgacggcacg ccattgcctg tggtggcgaa aggcgatgag ttgctgtgtg aaaaaggtga
    22681 agtggtagag cggcaaaccc agccgccgcg ccattttacc gatgcaacac tgctttcggc
    22741 gatgaccggg atcgcgcgct ttgtgcagga caaagatctg aaaaaggtcc ttcgtgcgac
    22801 cgatggtctg gggacagaag caacccgtgc cgggattatt gaactgctgt tcaagcgtgg
    22861 ttttctgacc aaaaaagggc gctatatcca ctccaccgac gccggaaaag cgttattcca
    22921 ttcgctgcca gaaatggcga cgcgaccgga catgaccgcg cactgggaat cggtgctgac
    22981 gcaaatcagc gaaaagcagt gtcgctatca ggactttatg cagccgctgg tggggacgct
    23041 atatcagctt attgatcaag ccaaacgtac gccggtgcgg cagtttcgcg gcattgtggc
    23101 tccgggcagt ggtggcagtg ctgataagaa aaaggctgca ccgcgtaaac gtagtgcgaa
    23161 aaaaagtccg ccagcagatg aagccggaag cggggcgata gcgtaagcga gcgaatcttt
    23221 cgtgctattc gagtcatatt ctgaaatatc cagcggatca agaaagttcg ttggatattt
    23281 ttttgcatgg ataaaattat cgcctctaaa gtatgtaata acagggaatg tgatgaaaaa
    23341 agttttattg caaaaccatc ctgggagcga gaagtattct tttaatggct ggggaatatt
    23401 taatagtaat tttgaacggg tgattaaaga aaataaggcc atgctgcttt gtaagtgggg
    23461 gttttattta acatgtgttg tcgctgtaat gtttgtattc gcagcgataa catccaacgg
    23521 tttgaatgaa agaggcctga ttaccgcggg atgctctttt ctttatctat taattatgat
    23581 ggggcttatt gttcgggccg gttttaaagc aaaaaaagaa caactgcatt attatcaggc
    23641 taaaggtatt gagccgctca gtatcgagaa gttacaggcg ctacaattga tcgcacctta
    23701 tcgattctat cataagcaat ggtctgaaac gctagagttc tggccgcgaa agcctgaacc
    23761 tggcaaagat accttccaat atcatgtgct tccttttgac tcgatcgata tcatcagtaa
    23821 aagacgcgag tctttagaga atcaatgggg tatcgaagat agcgaaagtt attgtgcctt
    23881 aatggagcat tttctttctg gcgaccatgg agccaatacc tttaaagcaa acatggagga
    23941 agccccagag caggttatcg ccttgttgaa taaatttgct gtttttccct cagactatat
    24001 ctctgattgc gctaataatc gctccggtaa atcctcggcg aagctaatat gggcggcgga
    24061 attatcatgg atgatctcga tatcaagcac agcttttcaa aacgggacaa ttgaagaaga
    24121 actggcctgg cattatataa tgcttgcttc tcgaaaggcg cacgagttgt tcgaaagcga
    24181 agaagattat caaaaaaata gtctaatggg atttctttac tggcatatct gctgctatcg
    24241 cagaaagtta acggatgctg aacttgaagc gtgttatcgt tacgacaagc agttttggga
    24301 gcactacagt aagaaatgcc gttggcccat aagaaatgtt ccctggggag catcatccgt
    24361 taaatactca taaacgcctg aaattttgcc gggggcgctg cgcttgaccg gcctacatta
    24421 gagcgtaact gcaatgaatt gtgcccatct gtaggcctga taagcgtagc gccatcaggc
    24481 atttacaact taaatcacac cctgcgccag catcgcatcg gcaaccttca caaaaccggc
    24541 aatgttcgcg ccctgcacgt agttggtttg ctcaccttca ccaccatgct caacacaggc
    24601 atggtggata tccagcatga tgtgatgcaa acgtgcgtca actttctcgg ctttccagcc
    24661 caggcgcgca gcgttttgtg ccatttccag gcccgacgtt gctacaccgc cagcattagc
    24721 cgctttaccc ggtgcaaaca gtacgcctgc ctgctggaac agttcagtcg cttcgatggt
    24781 ggtcggcata tttgcccctt cagcgacggc tttaacgcca ttggcgataa gctgatgcgc
    24841 ggcgtcaaca tccagttcgt tctgggtggc gcaaggcagg gcgatatcaa ccggtacaga
    24901 ccacggctgt tggccttcga gatagaccag accaaattct ttggcgtaat ctgccgctcg
    24961 accatcgcgg ctggctttga tttcgataag acgtgccagt ttctctttcg tgaatccgct
    25021 ttcatcaact acagtgccgc tggagtctga cgcagtgatc acacgagcac caaattccat
    25081 cgctttttcg atagcgtact gggcgacgtt gccggagcca gaaacggaaa cgctcatccc
    25141 ttcaaaaccc ataccgtggc gttttaacat tgcttctgtg aaataaacca gaccgtagcc
    25201 ggtagcttcc gggcgaataa gactaccgcc aaatgaaagg cccttaccgg tgaagacgca
    25261 ggcggtattg ttggagagct ttttcatcat ccccgccata aagccgactt cacgaccacc
    25321 aacgccgata tcacctgccg gaacgtcggt atccgcgccc aggtggcgat acagttcagt
    25381 catcagcgtc tggcaaaaac gcatcacttt accttcgctt tttcctttcg gatcgaaatc
    25441 gctgccgcct ttaccaccgc ccatcggcag ggtagtcagg gcatttttga aggtttgttc
    25501 aaagccgagg aatttgagaa tggaaaggtt aactgacgga tggaagcgca taccgccttt
    25561 gtacgggccg atggcagagc tgaactgcac acgccatgca cggttgacct gtatctggtt
    25621 gcgatcatca acccatacca cgcgaaactg gatcacgcgc tccggttcaa ccagacgctc
    25681 cagta
//
LOCUS       DAJTRD010000057        24181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTRD010000057 DAJTRD010000000
VERSION     DAJTRD010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.057"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(22..594)
                     /locus_tag="OO315_002511"
     CDS             complement(22..594)
                     /locus_tag="OO315_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5891379.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            812..1672
                     /gene="tesB"
                     /locus_tag="OO315_002512"
     CDS             812..1672
                     /gene="tesB"
                     /locus_tag="OO315_002512"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5891380.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO315_002513"
     CDS             complement(1721..3007)
                     /gene="amtB"
                     /locus_tag="OO315_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5891381.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO315_002514"
     CDS             complement(3037..3375)
                     /gene="glnK"
                     /locus_tag="OO315_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5891382.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(3556..5337)
                     /locus_tag="OO315_002515"
     CDS             complement(3556..5337)
                     /locus_tag="OO315_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5891383.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(5330..7102)
                     /locus_tag="OO315_002516"
     CDS             complement(5330..7102)
                     /locus_tag="OO315_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5891384.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO315_002517"
     CDS             complement(7132..7590)
                     /gene="decR"
                     /locus_tag="OO315_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5891385.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO315_002518"
     CDS             complement(7744..8562)
                     /gene="cof"
                     /locus_tag="OO315_002518"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5891386.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            8662..10354
                     /locus_tag="OO315_002519"
                     /pseudo
     CDS             8662..10354
                     /locus_tag="OO315_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            10419..11114
                     /gene="queC"
                     /locus_tag="OO315_002520"
     CDS             10419..11114
                     /gene="queC"
                     /locus_tag="OO315_002520"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5891387.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO315_002521"
     CDS             complement(11166..11564)
                     /gene="fadM"
                     /locus_tag="OO315_002521"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5891388.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(11658..12029)
                     /locus_tag="OO315_002522"
     CDS             complement(11658..12029)
                     /locus_tag="OO315_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5891389.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO315_002523"
     CDS             complement(12180..14051)
                     /gene="ppiD"
                     /locus_tag="OO315_002523"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5891390.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO315_002524"
     CDS             complement(14243..14515)
                     /gene="hupB"
                     /locus_tag="OO315_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5891391.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO315_002525"
     CDS             complement(14724..17078)
                     /gene="lon"
                     /locus_tag="OO315_002525"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5891392.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO315_002526"
     CDS             complement(17266..18540)
                     /gene="clpX"
                     /locus_tag="OO315_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5891393.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO315_002527"
     CDS             complement(18666..19289)
                     /gene="clpP"
                     /locus_tag="OO315_002527"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5891394.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO315_002528"
     CDS             complement(19536..20834)
                     /gene="tig"
                     /locus_tag="OO315_002528"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5891395.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO315_002529"
     CDS             complement(21178..21495)
                     /gene="bolA"
                     /locus_tag="OO315_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5891396.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            21800..22378
                     /locus_tag="OO315_002530"
     CDS             21800..22378
                     /locus_tag="OO315_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5891397.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            22422..23897
                     /gene="ampG"
                     /locus_tag="OO315_002531"
     CDS             22422..23897
                     /gene="ampG"
                     /locus_tag="OO315_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5891398.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5666 a         6664 c         5827 g         6024 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttggct
      781 tgctgcggca gctttgttac tggagagtta tatgagtcag gcgctaaaaa atttactgac
      841 attgttaaat ctggaaaaaa ttgaggaagg actctttcgc ggccagagtg aagatttagg
      901 tttacgccag gtgtttggcg gccaggtcgt gggtcaggcc ttgtatgctg caaaagagac
      961 cgtcccggaa gagcggctgg tacattcgtt tcacagctac tttcttcgcc caggcgatag
     1021 taagaaaccg attatttatg atgtcgaaac gctgcgtgac ggtaacagct tcagcgcccg
     1081 ccgggttgct gctattcaaa acggcaaacc gattttttat atgacggcct ctttccaggc
     1141 accagaagcg ggtttcgaac atcaaaaaac aatgccgtcc gcgccagcgc ctgatggcct
     1201 gccttcggaa acgcaaatcg cccaatcgct ggcgcacctg ctgccgccag tgcttaaaga
     1261 taaattcatc tgcgatcgtc cgctggaagt ccgtccggtg gagtttcata acccactgaa
     1321 aggtcacgtc gcagaaccac atcgtcaggt gtggatccgc gcaaatggta gcgtgccgga
     1381 tgacctgcgc gttcatcagt atctgctcgg ttacgcttct gatcttaact tcctgccggt
     1441 agctctacag ccgcactgca tcggttttct cgaaccgggg attcagattg ccaccattga
     1501 ccattccatg tggttccatc gcccgtttaa tttgaatgaa tggctgctgt atagcgtgga
     1561 gagcacctcg gcgtccagcg cacgcggctt tgtgcgcggt gagttttata cccaagacgg
     1621 cgtgctggtt gcctcgaccg ttcaggaagg ggtaatgcgt aatcacaatt aaaaaaatag
     1681 ccggaggtga aaaccgtccg gctatttttt gcagtgcttg ttacgcgtta taggcattct
     1741 cgccgtggct gttgacgtcc agcccttctc gctcttgttc ttccggcaca cgtaggccaa
     1801 ccgtcagatc cgccagtttg tagccaataa atgccaccac accggaccag acgatcgtaa
     1861 tggcgatgct ttccaactgt accagcaact gatggcccat cgtcacacct tcagcgaagc
     1921 ccacgccgcc cagagaactg gcggcaaaaa tcccggtcat gatacagccg acaatgccac
     1981 aaacgccgtg cacaccgaag acatcgcagg gatcatccac ccgcagcaag cgtttgagca
     2041 tggtaacgcc ccacaaaccc gccagaccag ctaccacgcc gataatcaac gcgccgccaa
     2101 ccccaatgta gccgcacgct ggcgtcacgc cgaccagacc ggcaatcgcg ccagaacacg
     2161 ctcccagcag agaaggctta ccacgcagcg cccattcacc gaagatccag ccaagaattg
     2221 ccgccgccgt tgcgaccaca gtattcacaa atgccagtgc cgcgatttca ttcgccgtgc
     2281 ccactgaccc ggcgttaaag ccaaaccaac cgatatagag aatggcagtc ccggtgaaga
     2341 ccatcggcag gttgtgcggt ttaaacgcct ctttaccgaa gcctacgcgt ttgcctatca
     2401 gatacgcgcc caccagaccg gcgattgctg cgttaatgtg caccaccgtg ccacccgcga
     2461 aatccagcgc accgtgagaa gccagcaaac caccgcccca caccatatgc gcaatcggaa
     2521 tgtaagagag cgtcagccat accaccacga aaatcaacac agctgagaag cggattcgtt
     2581 ccgccagcgc cccaactatc aagcctacgg taatgcaggc aaacgatccc tgaaacgcta
     2641 cgtggatata ctgataaatg ctgcccatca ccgccgtcag ttcgatgttt tttagcatca
     2701 accagttaat gttgccgaag aagttgttgc cctcaccaaa cgccagcgaa taaccgtaaa
     2761 ccacccagag aatacagacc agtgcaaacg tcaccgtcac ctgcgtcagc atcgacagca
     2821 cgtttttgcc gcgaatcaac ccaccgtaaa acagggcaat ccccggaata gtcataaaca
     2881 gcaccagcgc agtacaaatc atcataaacg cattgtcggc tttatcggcc accgcaggtg
     2941 cagccattac cagtcccggc agcatcgcca gtgaagcaag cccagttttt atcgtcgcta
     3001 tcttcatttt tcgttcctgt tgctgtgtgc cagagattac agcgccgctt cgtcggcttc
     3061 gccggtacga atacgaatga cgcgttgcaa ttcagcgacg aagattttgc cgtcgccaat
     3121 ttttccggtg taagccgcct tactgacgat atcgatcact tcatcgagtt ggtcatcagc
     3181 aatcgccaca tcaattttta cttttggcag gaagttgacg ctgtattccg ccccccggta
     3241 cagctcggca tgccctttct gacgcccgaa acctttcact tcggtgacgg tcaggccctg
     3301 aataccaatg gaagataacg cttcacgaac gtcttccagc ttgaatggtt tgattatcac
     3361 ggtcaccagc ttcatagatc ccctccggtc agaattcggt aatggttctg ctacacgaag
     3421 aaggtattgc aagcggtgtg ccagaaatga aaaacgagaa agagagcagg aagttaagaa
     3481 gattgaaaat gaaaacgcac tatgacagtg cacagtgcgt tacatttttg caccaacgat
     3541 gaaatattgc gctattcagg cgctcaatga ttcctcttca cgcacgctgg ctgccagctc
     3601 ttcgcccgca agttgcagtt gatacatctg ccagtagcgt ccctgggccg ccagcagttg
     3661 ctggtgagtg ccctgctcca cggcttgccc acgatgaagc accagaatgg tgtcggcatc
     3721 aataatagtt gataagcggt gagcaatcac caccagcgtg gtatgttcac gcaccgccgc
     3781 cagagcatgt tgaatcgcct gttcagtacc ggagtcaatg ctggcggttg cctcatcaag
     3841 gatcaggatt tgcggcgtct ccaccagcac gcgcgccagt gccagcagtt gcttttgccc
     3901 gactgagaga ttatttccct gctcgcccag cggcgtataa atacaatcac tcatgctgcg
     3961 cgccagctcc gccagttgca cggtttccag cgcctgccag acgcgttctt cggagatatc
     4021 ccgccccagc gtcacattag cgaggaaggt atccgccagc accaccggat cttgctgcac
     4081 cattgccacg ccctggcgca gcgcgctatg acttagcgaa cttaatggac gaccatcaag
     4141 gtgaatctca ccttccgtta gcgggtaata gcccatcaat aaactggcga gggtactttt
     4201 accactgccg gtatgcccga ccagcgccac aaagttacgc gaaggcacag agagattaat
     4261 gttctttagc accagattgt catcgcgata agcaaatgac acgttatcga cttcgatggt
     4321 gccactctgt aacgggtaat catcattgcc atattgctgg cgcggtccgt ccatcagttc
     4381 aaacacgcgc tcaccagcaa caacagcctg ttgcagcatc gcctgttgcg tggtcagttc
     4441 gattaagggt tcgttaagtc gcccaagata gctgataaac gcatacaaca cgcccacttc
     4501 aatggtgccg ctggcggaga agccaaacag catcaataag ccacaaagaa tgagcgatga
     4561 aaacagactc agcagcggac gcagcagaaa accgtcgagg cgcagggttt gcatcctagc
     4621 catatagtgt gaacgactgg cctcccccat acgttcgcca aatcgcgcct gctgacgaaa
     4681 ctgctggata acgctcatgc cattgatgat ttcgttaaag ccgtcgttga tatccgccag
     4741 ataggcgcgc acacggcgga caatcggcgt gctgtaacgc tggtatatca ccattaccac
     4801 cagcaccacc gggaaaatca ttatcgccac cagcgccatt cgccagtcga ggctgaacat
     4861 cgccaccagc atcgcgccca ccagtgcggc actgcgcagg acagttgcca ctacggtaac
     4921 gtagagatcg cggatcactt cagtgtcatt agtgacgcgg gaaatcacct gcccgacggg
     4981 ttgggtatca aactcgctta atggctggcg taacgcagca tccatcacgt cggtacgcaa
     5041 ctgttgcact gcgcctactg ccgcccgatt aaacagcagc gactgcgcgt aatgtagccc
     5101 ggcggcaaac agttgtagcc caacatacgc cgcagccagc cctgcaacca ctttcaacgg
     5161 caggttattt ttcgctacca tattgtcgat aaaatagctg ataagcagcg gcccactgac
     5221 ttctgccgcc gccgcaaccc acatcatcag gaccgcaatc cccagcggtt tacgccacgg
     5281 cgaaccatac gctaacaggc gcttgagagt cggccacagt tggctaaaac tacgcatcga
     5341 cggcctcctc gcgaatttcc ggaacgtcgt cgagcgccgc ctccagttgt tgatagcgat
     5401 acatatcgcg ataccaaccg ctttgttgcg ccagctcatc atgattgcca cgctgggcga
     5461 tatgtccgtg ctgcatcaca ataatttcac tggcttccgt cagtgccgaa aggcgatggg
     5521 cactgatgat taccgttctt ccctgtcccc actgacgcag gttatgcagg atctggtgct
     5581 ctgtgcgtcc gtccaccgcc gaaagcgcat catcaaggat gaggatttcc gcgttgacta
     5641 ataacgctcg agcaatggag atacgctgtt tttgcccacc ggaaagcatc acgccgcgct
     5701 cgcccacttc tgtatcgtaa ccttgcggta gacgcaaaat atcgtcatgt acgctggcta
     5761 accgcgcgac atgctcaatc tcttgctggg tggcattcgg gcaacccagc gcgatgttat
     5821 tcgccacggt gtcagaaaaa aggaatggcg tctggctgac caccgccagg cggctacgcc
     5881 agctatcgag ttgtaacttc gtcagaggaa tatcatgaaa gcgaatatcc ccctcgctga
     5941 cgtcgaaatg acgctgaatg agcgacaaca gggtactttt gccggaacca gtcggcccgc
     6001 aaatacccag catctgaccg ggtttcaggg cgaaattgac gttttccagc gcaggatggt
     6061 cagtctgcgg atacgtgaac tggtgaatat ttacatccag ttcgccacgc ccttccggca
     6121 cgggttcgct accatcgttc accaccggcg cttccgccag catggcgcga atacggctgt
     6181 atgcagcact accacgttcc acaatgttaa acatccatgc cagcgccagc attggccaaa
     6241 tcatcagacc taaatacatc ataaaactgg tgagctggcc cagcgttaaa ctgccctgca
     6301 ccaccatcca actaccgccg ccaatcgcca gcaagttcgc cataccaatc gcgatataga
     6361 tggtcgggtc gaagcgagca tcaatacgcg ccacccgcat gttttttttg ccggtatctt
     6421 cggcatccgc ggcaaataac gccgactggc gatcttccag accaaaggct ttgatcatgc
     6481 ggatactggt gaggctttcc tgggtgcggt cattaagact ggaaaacgcc gcctgtgcca
     6541 gcttaaagcg ttcatgcaag gcatcgccgt tgcgcttaat catgatcgcc atcactggca
     6601 tcggtaacag ggcaaataag gtcaactgcc agctaatttg cgtagacatc ataatcaaca
     6661 cagcgcagcc cattaccagt gaatccacca gcgtcagcac cccttctccg gcggcaaaca
     6721 cgacgcgatc gacgtcattg gtcgcacgag ccatgagatc acccgtgcga tgacgcaggt
     6781 aaaactccgg gtgctgccgg cttagctgac ggtaataatc ttcacgcagt tcaacagcca
     6841 gttggtaaga cgcgccaaac agcaatacgc gccagacata acgcaggaga taaaccacaa
     6901 cggcaatcag caccatggtg gcgatccaca tcaggctcag cccggtagta aagtgttgtt
     6961 ctgtcacgcc atcgacaaca ataccaacca cttttggcgg aaccagttgc agcatcgcga
     7021 taatgacaag caaggcgaca gccccaagat agcgacgcca ttcccgacgg aaataccagc
     7081 ttaattgagc aaataatcgc acgcgttgtg tcctgacctg attctggata tttattcgat
     7141 gggtaaagaa gtggtgtatt taatctgttc catcgcgaag ctggaagtga cgtccgacag
     7201 ccccggcacg ctgtttacca gacgcttata aaactcgtcg tagcgtttca tgtcggcaac
     7261 ctggacgcgc atcagataat cgtattcacc agccatgcgc cagaccccca gcacttctgg
     7321 catttcggta accaccgtga caaagcggca ataccattcg ctgctgtgat gttgcgtttt
     7381 tatcagcaca aaggcggtca ggccgaggcc tattttttcc ggatccagca gggcgacttt
     7441 gccgataagg ataccgtcgt cctccagccg tttcaggcgc ttccagcagg gggtggttgt
     7501 cagattaacg gcttcagcca gtgcctgcaa agagagggtg caatcctgct gcagtaaggc
     7561 cagcagctta cggtcaattt tatctaacat agcccttcca cggagaattt ttttctcttt
     7621 taattctatt ttaaaggtga aatcgcgaca atttattctg tgcggtttaa acaccacggc
     7681 acaaaatgac aaattcaacg caaaaaaaaa ccgggcaatt gcccggtgct ggaagggatc
     7741 tcgttattcg ggggaataag gtagatgtgg atagtccagc cagtgcgtca aatagtgaga
     7801 gacagcctga tttcggcaat gtccaatcac cggtaaatgc gggagctccg cgcgcagttg
     7861 cggcatcgca ttgcccataa taaacccgct accgacgctg cctaacattt cgcgatcgtt
     7921 catcgcatca ccaaaggcca tgcaatcgcg caacgataaa cctaaatgtt gggtccgcac
     7981 cgtcaatgca gcgcctttat tgcagcccac cggcagcact tcgaggcaat ccgtggcgga
     8041 aaaacacaaa tgtgcacgct cgcctaatgc ttcgtatagc tggatctgca agcgtgtaag
     8101 atcgtcgtga tcgccacaga agcagatctt ggtgacgctg ccgagtggca tttttttgac
     8161 atcgattatc tgataacgaa aaccgctata gacaaatacc tgcaacaacg cagggctctc
     8221 tttcccggta aaccaaccgt cgtcattgaa gatatgcatg ctggctcggg tatcccattg
     8281 ctgatacagc accagctccg cgacatccgc aggtaaatca tcacgatgta aaagttcacc
     8341 ttccagagaa tgcacgcgcg ttccgttgcc ggtaatcaaa tatgcatcca gcgatagcgc
     8401 cccgagaata tgctgcatct ccagcgcatg acgccccgtg gcaaaagtga gggtaatgtc
     8461 gcgttcacgc agtcgcgcca aagtagagag ggttttctca cctaaatgat ggtcaggcat
     8521 caataaagtg ccatccatat caaatgctac cagacgagcc atttctttct ccactctgtg
     8581 acacggttaa tggtgattga gtatcacctg atatatacgg aagtaatagt gaatagttaa
     8641 ataatattgt tccgggttta tatgcgattg ctcaaccgtc ttaaccagta tcaacgtctg
     8701 tggcaacctt cagccgcaaa ccgtcaccgt cagcgaactg gccgaacgct gtttttgcag
     8761 cgaacgccat gttcgtacgc tgttgcgtca ggcacaggag gcgggatggc tggagtggca
     8821 ggcgcagtca ggacgcggaa agcgcggaca attacgcttt ctggtcacgc cggaatcgct
     8881 acgcaatgcg atgatggaac aggcactgga aaccggaaag cagcaagatg tgctggagct
     8941 ggcgcaactg gccccaggtg agctgcgcac tctgttacag ccatttatgg gcggacaatg
     9001 gcaaaacgat acacccacgt tgcgtattcc ctactatcgc ccgctcgaac cgctacaacc
     9061 aggctttttg cccggccgtg ccgagcagca tctcgccggg cagatatttt ccggcctgac
     9121 ccgcttcgat aataatactc agcgcccgat tggcgattta gcgcatcact gggaaacctc
     9181 tactgacagg ttacgctggg acttttatct tcgttcaacc ctacactggc ataacggcga
     9241 tgcagtaaaa gcctcacact tacaccagcg attattgatg ctgttacaac tgccagcact
     9301 ggatcaatta tttattagcg tgaagcgtat tgaagtcacc catccgcagt gtctgacctt
     9361 ctttttacat cgccccgatt actggcttgc gcaccggctg gcgagctatt gcagccatct
     9421 ggcgcatccg caattcccac tgatcggcac tggtcctttt cgcttaacac aattcacagc
     9481 ggagctggtg cgcctggaga gccatgatta ttaccatttg cgtcatccgc tgcttaaagc
     9541 ggttgagtac tggataactc cgccgctttt cgaaaaagat ttgggaacca gttgtcggca
     9601 tcccgtgcaa atcaccatcg gcaaaccgga ggagctgcaa cgggtcagcc aagtcagtag
     9661 tggcatcagt ttaggttttt gctatttaac gttgcgcaaa agtccccgac tctccctctg
     9721 gcaggcgcga aaagtgatct ccattattca tcaatccggt ttattacaaa cgttagaagt
     9781 cggagaaaac ctgatcaccg ccagtcatgc attactgcca ggctggacta ttccgcactg
     9841 gcaagtaccg gatgaagtca aactaccgaa aaccttgacg ctggtttatc acctaccgat
     9901 agaacttcat accatggcag aacgcctaca ggcgacactg gcagcggaag gctgtgaact
     9961 cacaattatt tttcataacg caaaaaactg ggacgacacg accctactgg cacacgcaga
    10021 cctcatgatg ggcgacagat taattggcga agcaccggaa tatactctgg agcaatggct
    10081 acgttgcgat ccactatggc cacatgtttt cgacgctcca gcatatgcac atctgcaatc
    10141 gacgctggac gcggtgcaag taatgcctga tgaggaaaac cgatttaatg ccctgaaagc
    10201 ggtttttagc cagttaatgg cagacgcgac gctgacgccg ctgttcaact atcactatcg
    10261 cattagtgcc cctcccggcg tgaacggtgt gcgactgaca ccgcgcggct ggtttgaatt
    10321 taccgaagcc tggcttcccg cgccgtcgca atgaatagct ggtccgggtt aatcgcaggc
    10381 gttaccataa cgcttttatt gttcaagcag gattatctat gaaacgtgct gtcgttgtgt
    10441 tcagtggagg ccaggattcc accacctgtc tggtgcaggc attacaacaa tatgatgaag
    10501 tccattgcgt gacgttcgat tacggtcagc gacatcgcgc agaaatcgac gtggcacgcg
    10561 aactggcgct gaaactggga gcacgcgcgc ataaggttct tgatgtaacg ctgctcaacg
    10621 agctggcggt cagcagcctg acacgtgaca gcattccggt gcctgattat gaacctgaag
    10681 ccgatggcat cccgaatacg tttgtcccag ggcgtaatat tttgttcctg acgctggcgg
    10741 caatatatgc gtatcaggta aaagcagaag ccgtaattac cggcgtctgt gaaacggatt
    10801 tctccggcta cccggattgc cgcgatgagt ttgtgaaagc actaaaccat gccgtcagtt
    10861 tgggcatggc gaaagatatt cgttttgaaa cgccgctgat gtggattgat aaagcggaaa
    10921 cctgggcgct ggcagattat tacggcaaac tggatttagt ccgtaacgaa acgttgacct
    10981 gctataacgg cattaaaggc gacggttgcg gtcattgtgc ggcatgtaat ttacgcgcca
    11041 acggtttgaa tcattatctg gccgataaac cgacggtgat ggcagcgatg aagcagaaaa
    11101 ccgggttgag gtaattattc cgggtgtcgc cggatgcggc ttgagcatcc ggcaccacaa
    11161 aacgtttact taaccatctg ctccagcttt tcgcgcaatt ccccttccag agctaatgct
    11221 ttctgcgttt taagatcaat acaaacaaac gtaataagcg catccgctac cacctgccct
    11281 tccggctcca gtgtaatgac ctggtttaag atgccgcttt taccgtttaa ttgctgcaac
    11341 tgactggtaa tagttaacag gtcgcttaat accgctggac gacgatagtt aatattgata
    11401 ttgaccacga cgaaggcgat gttatgggcc gtcatccact gaaaactgtc gctattttcc
    11461 accccatccc agcgggcttc ttcgagaaat tcaaggtagc gggcgttgtt gacgtgctgg
    11521 taaacgtcga gatgatatcc acgaactttg atttgtgttt gcatagcgca ataaccttac
    11581 gttgttgtta taagtacaga ggtcataact ggtatgacct cttcagtctg gcaaaaaatt
    11641 gccactatgc aaattaatta cagggttaat accgccagat tacgttccac cagcgaattg
    11701 cccatccccg gcacctgctt tagatcctcc accgttttaa acggaccgta ctcttcgcga
    11761 taactgacaa tcgcctgcgc tttcttcagg ccaacgccat tcatcgcgcg ggctagctct
    11821 tccgcgctgg cattattaat gctgacccgg gtgccttctt cgtcactggc tttcgccggt
    11881 actgctgctt tactttgtgc tgcaggagct tccgctttgg tttctaccgc cgtcggtttc
    11941 gccacagaag ctgccgccag cgcgctatga gacattccgg cacaggccag ggacagtgta
    12001 atgagcagtg ctttaattcc gtgtttcata ctgttttctc cttgtttgtt aacagtggaa
    12061 tcaccttaac gggagtgaaa acagtaaaca aatggcaatg ttcagaaatg gaaaaggccg
    12121 cgaaagcgac cttttcttgt tacagctgca acgcattttt ttgcgaggcg gcttcaggat
    12181 tattgctgtt ccagcgcatc gccaattttg attttcgcct ctttacgcag gttactcatc
    12241 agagcttcaa agacgatttg tgcgttgttc tgggtgatac cctgcaccat cgcttttttc
    12301 tgatcttccg gcattgaacc ttgtttcact tcatccagcg ccagcagaac cacattacct
    12361 tgcatatcgg tcgccatacc gtagctcggt ttgtctttcg ctggcagtgg cagtgcaaac
    12421 gccgcctggc taatcgggtc acgaccggaa cggcttaagg ttttcggctc gccaaatttc
    12481 agaccggcag cctgcatagc ttccgcacct ttgccggctt tcaaatcaac cagcagtttc
    12541 tcagcatcca ctttcgcctg ttgttcagct ttgttgtgct gaaccagtgc cttaacttgt
    12601 tcctgaacat ctgccaacgg tttcaccgct tccggtttgt gctcgctgac gcgcagcacg
    12661 aatgcgcggt cgccgtctac ggtgatgatg tcagagttga tgcccgacgc gccgttttca
    12721 cctaccagac cgccgttaaa gatagcgtcg gcaaccggct tgaagttcaa ctcttccggc
    12781 aggttatctt tgctgaacca acccgtctga gtggctttaa cgccggcagc ttgctctgca
    12841 ccggccagag actcggtgtc gttgcttgcc gcatcgctca ctttctgctg cagcgcatag
    12901 tacgcatcga gggctttttc gtgtttcact ttcgccgcaa tgtcgtcacg tacttcgtct
    12961 aacgatttca ctttcgctgg ctgaatgtcg tccagacgta caatcaggaa accgaccgaa
    13021 gatttgatga caccagacag ttggcctttt tctttcagac cagcattttt cagttcgtcg
    13081 gggatagtgg catcttctaa ccaacccata tcgccgccgt tacgggcaga gataatatcg
    13141 gcagattttt ctttggctaa tgcagcaaaa tcaccgcctt tattcagctc atcaagtacc
    13201 gctttcgctt catcttcagt tttggtctgg atgatgctgt agcgggtacg ctgcggctgg
    13261 gtgaattgat cctgatgctg atcgtagtag ctctggatat ccgcatcgct aaccggttgc
    13321 tgcatcgttg cggcaaccag cttgatgtaa ctcacgcgga attgttccgg cgtcatgaaa
    13381 ttgtttttgt tttgttcgta gtagctggca atctcctgtt cggtcacagg ctgcttcgcc
    13441 gccagcgcgt taacatcgat agtcgcctca cgcaccacgc gttgttgcgc gaccagtgcc
    13501 gccagctcgt cggtttcacc tttcagcata aaatcggtac cggcaacgcc gttaatcagc
    13561 tgttgggtag tgagctggtt acgcagcgcc tgggcgtact gatcggcggt catccccatc
    13621 tggttgagga taccgttata gcggctgtta tcaaatttgc cgtcaacctg gaaggctggg
    13681 gtcgcgaaaa tcgcctgttt aacctgctca tcactgatac ccagtttcag ctcacgagcg
    13741 tactgatcca acagcgcctc gtcgatcaga cgattcagca cctgttgacg cagggttttc
    13801 atatagcctt cgttcgctgc cagctcggag tattgatcgc ccagctgttg ctgcatacga
    13861 ttacgctcgc tgttaaaggc gttctcgaac tgcccacggc tgatttcctg gtcattcact
    13921 tttgcggcgt agttattgcc tccgccaatc aggtaaccac tcacgccggt caatatgaac
    13981 gacacgataa tgataccgaa aataatcttg agcacgagac tgtttgcagc cgtgcgtaag
    14041 ctgtccatca tggtgtaaca acactccgct gtagatgact gtgtacctca cgcaacgtat
    14101 cgatgcgcgc aaaggcctat tgtgacaaga aacgggggca attgtcagcc cacaagacac
    14161 agaaagtcac cgaatacaaa taaaaaaggc acatcagtag atgcgccctt gaacttcgtc
    14221 acatccccac tggggacaac gcttagttta ccgcgtcttt cagtgcttta cctgcacgga
    14281 agctcggtac tttagcggca gcgatggtga tctctttacc ggtctgcggg ttgcggccag
    14341 tacgggcagc acgctcttta acggcaaaag taccaaaacc taccagtgct acatcatccc
    14401 cttctttcag agattcagtt acggaagcaa taatagcatc taacgcacgg ccagccgcag
    14461 ctttagagat atcagcccct gcagcaatct tgtcgatcaa ttgagattta ttcactcttc
    14521 tcttcctctt tataatttat atcgcacttg aatccttcaa ggtacgaacg cgcagcagtt
    14581 atatcaggcc agccatcccc ttacaacacc ccttaataag ggcaagcccg atccgctatc
    14641 taacttagcg agacaaaaaa aggctggcaa gcccgaatta gcctgccagc cctgttttta
    14701 ttagtgcatt ttgcgcgagg tcactatttt gcagtcacaa cctgcatgcc agacggttca
    14761 ttttgcagcg ccagagtcag aacttcctca atgcgcttca caggatgaat gtccagatcg
    14821 gcaattacgt tgtcaggaat ctcttccaga tcgcgtttat tttcgaacgg aattagcact
    14881 gttttaatcc cgccgcgatg cgctgccagg agtttttctt tcaaaccacc gatcggcagt
    14941 acctgaccac gcagagtgat ctcaccggtc attgccacat cggcacgaac cgggttaccg
    15001 gtcaggcaag aaaccagcgc ggtgcacata gcaataccgg cactcggacc atctttcggc
    15061 gtcgcacctt ccggtacgtg gacgtggatg tcacgttttt cgtaaaaatc agggttgatc
    15121 cccagttttt ccgcacgcgc acgaaccacc gttaacgccg cctgaataga ctcctgcatc
    15181 acttcgccga gcgaaccggt ataggtcagt ttgcctttac ccggaacaca tgcggtttca
    15241 atggtcagca agtcaccgcc cacttccgtc cacgccagac cgattacctg accgacacgg
    15301 ttttcgttat cagcgcgacc atagtcgaaa cgctgaacac cgaggtagtc atgcaggtta
    15361 tcgccgttaa tttcgatatg ttttaatgac ttatcgagca gtaactgctt aaccgctttg
    15421 cggcacagtt tggagatttc acgctccaga ccacgcacgc ccgcctcacg ggtgtagtaa
    15481 cgaataatgc cgataatggc gctatcgtcg acggtcagct cacctttttt cagtgcatta
    15541 cgttcaatct gcttcggcag caggtgacgt ttggcgatgt tcagtttttc atcttcggta
    15601 taaccggaga ggcgaatcac ttccatacga tccagcagcg gtgccggaat gttcatggag
    15661 ttcgacgtcg cgacaaacat cacgtcgctg agatcgtaat ccacttccag gtagtggtcg
    15721 ctgaacgcta cgttctgctc tggatccagc acttcaagca gtgcagaggc cggatcgcct
    15781 cgcatgtcag aagacatttt gtcgatctca tcgagcagga acagcggatt tttcacgccc
    15841 actttcgcca ttttctggat cagtttaccc ggcatagaac cgatgtaagt acggcggtga
    15901 ccacggattt ccgcttcatc acgcacgccg cccagcgcca tacggacata tttacgcccg
    15961 gtggctttgg caatggactg accaagagag gttttaccta cccccggcgg ccctaccagg
    16021 cagaggatcg gtcccttgat tttgttgaca cggctttgaa ccgcaaggta ctcaaggatg
    16081 cgatctttca cgcgctccag accataatgg tcggtatcaa ggatttcctg cgcctgacgc
    16141 aggtcttttt tgaccttgct gcgcgcattc cacggtacct gtaccatcca gtcgatataa
    16201 ccacgcacta cggtcgcttc tgccgacatc ggagacatca ttttcagctt ctgcaactct
    16261 gcttccgctt tctcttttgc ctctttcggc atcttcgccg cgtcgatttt gcgcttcagg
    16321 gcttcgtttt cgtccggcac gtcgtccatt tcaccgagtt ctttctgaat agctttcatt
    16381 tgctcgttca gatagtactc acgctgggat ttctccatct gctttttaac gcggttgcga
    16441 atgcgtttct caacctgcag cagatcgatt tccgattcca tcattgccat cagatattcc
    16501 agacgttcgt taacgtcgga catctccaga acggactgtt tgtcagccag tttcagcggc
    16561 atatgtgcag caatggtatc cgccagacgc gccggatcgt cgatgctatt cagcgacgtc
    16621 agcacttctg gtgggatttt tttgttcagc ttgatgtagc cttcgaactg gctgattgca
    16681 gtacgcacca gcacttcctg ttcccgctca tcaatggtcg gcgactccag atactccgcc
    16741 ttcgcagaaa agtgttcgcc attgtcagag agcgcagaaa tacgcgcgcg ctgtaacccc
    16801 tcgaccagca ctttgacggt gccgtcaggc agtttcagca tctgcaatat agaggccacg
    16861 gtcccgacgg tgaaaagatc gtttacaccc ggctcatccg ttgaagcttc tttctgcgcg
    16921 accagcataa tttttttatc atggtccatc gccgcttcca gacaacggat agatttttcc
    16981 cgcccgacaa ataaggggat gaccatgtgc ggataaacca ccacatcgcg cagcggcaat
    17041 acggggattt caatgcgttc agaacgctca ggattcatag agctctctct tagtttaatt
    17101 tccgccaggt aatcagatga cacgactgtg cttcacgcca tctattaaca tgtacgtcag
    17161 tatatgggga tgtttccccc acattcaacg ccgagaatag aggaaaaatt aaaggggaga
    17221 taaaatcccc cctttttggt taactaattg tatgggaatg gttaattatt caccagatgc
    17281 ctgttgcgct tccggcttac cataaatcag caacggtttg ctttgaccat caattaccga
    17341 ctcgtcgata accacttttt cgacgtcttc catggacggc agatcgtaca tggtatcgag
    17401 cagtgcggct tctacgatgg aacgcaggcc acgggcaccg gttttacgcg ccatcgcttt
    17461 cttagcgata gcatccagcg cctcgtcacg gaattccaga tccacgcctt ccagattaaa
    17521 cagcgcctga tactgcttgg tcagggcgtt tttcggctct ttgaggatct gaatcagagc
    17581 ttcttcgctc agttcattca acgttgctac aaccggcaga cgaccaatga actcagggat
    17641 aagaccaaac ttgatcagat cttccggttc aacctgcgcc agcagctcgc cttcgcttgc
    17701 tttgtcggac ttcgctttta ccgtcgcgcc aaaaccaatg ccggagccgg tttctacacg
    17761 gtgggaaatc actttatcca gaccggcaaa cgcaccgcca caaataaaca ggatcttaga
    17821 ggtatcaacc tgcaagaatt cctgctgcgg atgtttacgc ccaccttgcg gtggaacagc
    17881 agctaccgta ccttcgatca gtttcaacag tgcctgctgt actccttcac cggaaacgtc
    17941 tcgggtaatg gacgggttgt ctgacttacg agaaatcttg tcgatttcat cgatgtagac
    18001 aatcccgcgc tgcgctttct gtacgtcgta atcgcacttt tgcaacagct tctgaatgat
    18061 gttttcaacg tcttcgccca cataaccggc ttcggtcagc gtggttgcgt cggccatggt
    18121 gaacgggacg tccagcaggc gcgccagcgt ttcagccagc agcgttttac cggaaccggt
    18181 cggaccgatc agcagaatgt tacttttgcc caactcgaca ccattgctgg tatcgccgtt
    18241 gcgcagacgt ttgtaatggt tgtataccgc gaccgccagc acttttttcg cctgttcctg
    18301 gccgataacg taatcgtcca ggtggttgcg aatttcatgc ggcgtcggta gcgcactgcg
    18361 ttcacgatgc ggtgcaactt ctttaatctc ttcgcgaatg atgtcgttac ataaatcaac
    18421 acattcgtcg cagatataca cggatggacc ggcaatcagc ttgcgcactt catgctggct
    18481 tttgccgcaa aaagagcaat acagcaattt gcctgagcca tctttgcgtt tatctgtcat
    18541 gggtcaaaac ctcttctttg ttctttgtgc cgcacacgac gacgcaaatg ccattctcag
    18601 gcgcaagccg cttacagcgt tgtgccgccc tagataagta tagcggcaca gttgcgcctc
    18661 tggcatcaat tacgatgggt cagaatcgaa tcgaccagac cgtattccac cgcttcaggg
    18721 gcggaaagga agcgatcgcg ctcggtatca cgttcaatct gttctaatga ttgacccgta
    18781 tgaagcgcca taagttcatt catgcgccct ttaactttca gaatttcacg ggcatgaatt
    18841 tcgatatcgg tcgcctggcc ctggtagccg cccaacggtt ggtgaatcat cacgcgcgaa
    18901 ttcggcaggc aaaaacgttt accttttgcc cctgcggtca acaagaaagc gcccatcgag
    18961 gccgcctggc ccatacagat ggtgctgaca tcaggcttga taaactgcat ggtgtcatag
    19021 atagacatcc cggcagtgat caccccgcct ggggagttaa tgtacagata gatatctttt
    19081 tctgggtttt ccgcttccag gaacagcatc tgcgccacaa tcaggttagc catgtggtct
    19141 tcaacctggc cagtcagaaa aatgacgcgt tccttaagta gacgagaata gatatcaaaa
    19201 gagcgctcac cgcgtgaggt ctgttcaatg accatcggca ccagcgccat atggggtgca
    19261 aagttatctc gttcgccgct gtatgacatt tccgtctcct ggataaaatt gaaaaaaacc
    19321 tgctgtaccg attgtaacct tatggacgga ttatcagcta gtccctgtgt tatgggtacg
    19381 gcatcaccat atttcaagca taacaatctt ttgttgttac gctaacaccg aaagaggctt
    19441 ttcgcacggc tccatgcaaa attcatgaca aaaaaaagcc caccacctgg cggtgacggg
    19501 cctttgtgcg aatttagcgc gttatgctgc gtaaattacg cctgctggtt catcagctcg
    19561 ttgaaagtgg tttctttttc agtcactttc gctttcgcca gtacagcttc aacagcctgt
    19621 tcttccagag caacattgcg catgttgtcc atcagttctt tgtttttgct gtagaactcg
    19681 ataacttctt tcggatcttc gtacgcagaa gccatctctt cgatcaggcc tttcacgcgc
    19741 tcttcgtcag ctttcagctc gttggtgcgg ataacttcgc ccagcagcag gccaacaact
    19801 acgcggcgtt tagcctgttc ttcgaacagt tcgcgcggca gttccagagc ttgtttttcg
    19861 ttgccaccga aacgctgtgc agcctggcga cgcagaacgt cgatttcgct gtcgatcagc
    19921 gcagccggta cgtcgatgtc gttagctttt accagacctt cgatcgcctg agacttaacg
    19981 cggttacgga tggcgctctt cagctcgcgc tccatgtttt tacgcacttc agcgcgcaga
    20041 ccttctacgg aaccatcttc aacgccgaaa cgtttgatga attctgcagt cagttccggc
    20101 agttcacgct cttcaacttt cttcaggttg atagcgaatt tcgctgcttt acctttcagg
    20161 ttttctgcgt ggtattcttc cgggaaggtc acgtcgatgg tgaactcttc gccagctttg
    20221 tggcctttga taccgtcttc aaagcccggg atcatacgac cctggcccat cgccagtacg
    20281 aaatcagacg ctttaccgcc ttcgaactct tcgccgtcta cagaaccggt gaagtcgatg
    20341 gttacgcggt cttctgcttc aacagcgccg tctttttctt tccaggtcgc ctgctgttta
    20401 cgcagagtat ccagcatgcc gtcaacgtca gcgtcggtca cttcaacgat cggtttttca
    20461 acttcgatcg cttccagacc ctgcagttca acttccggat aaacttcaaa ctctacagag
    20521 taagtgaagt cttcacccag cttgtattcg cccggaacat aagtcggtgc gccagccgga
    20581 ttgatttttt ctttaatgat ggcgtcaatg aagttacggc tcatcaggtc acccagaacg
    20641 tcctggcgta cagacgcgcc ataacgctga gcaacgatat tcattggcac tttgcctttg
    20701 cggaagccgt caatacgtac ttttttcgca acgttgacca gctcgctttt aacagcggtc
    20761 tcgatgctgt cagcagcgat agtaatcgtt acacggcggc caaggccttg agtggtttca
    20821 actgaaactt gcatcttgtt acctcaaaaa atcacagtgc tcggtcaact cttaccccgc
    20881 aagcacaggt tgttggcttc gcggaaccgg gatgttctta taatcaatca cattccctgt
    20941 taccagaatc atcccgaaag cattcaaata ggacgcggca ttatagcggc atcactttta
    21001 tgagtcgaga acggttattg cgcgctgtcg cgtatttttt cacaattccc gggcaatttt
    21061 cgtctgaatg ctgggcaaaa agcgcaaaat ccagacaaaa caaaacggcc cgcaggccgt
    21121 ttttcataaa atctttagca acatactgga aaagcgccga cagttgcaaa tgcgttttta
    21181 cgcgatgctt cctgctccac gacagggagg agaggcaaag acggtgtcct gcaacccttc
    21241 ccactcctta atagtgtaag tatgcagagc cagcgcatga acggtagtag agagttcctc
    21301 cgctaaagta ctgtaaatca ttcgatgacg attcagaaaa cgttcacccg taaaacgatc
    21361 gctgaccagc acaactttaa aatggctttc agagccggct gggacattgt gacgatagct
    21421 ttcatccact acttcgagga atacgggttg gaacgccgcc cttaattttt cttctatccg
    21481 ctcacgtatc atcatgaaat atctcctccg acaacgctga gatgtcatcc atccctttaa
    21541 atactagccg cttttaccgt ttccattgca gcttaacaac aaatatttag gtttcgtcct
    21601 ggtttttccg tcaagcgtat gaagtttact gatttaaaga ttgaaacaca ggcctcgcat
    21661 cagcgtttct tttaccaggg gtgattattt cgtcaacatg atgaatttac atgccttacc
    21721 cacttcccct cgccgttggc aatgttatga tggcgggaat ttttaccaaa cacgcttaag
    21781 agtctctgag agcccgaaca tgttcaaaaa aatcctcttc ccgttagttg ctctgtttat
    21841 gcttgcagga tgcgcaaaac cgccaacaac tattgaagtt tccccgacga ttacactgcc
    21901 acagcaggat ccaagcctga tgggcgtcac cgtaagcatt aatggtgccg atcagcgtac
    21961 cgatcaggcg ctggcaaaag tcacccgcga taatcaaatc gttaccctga ccgcctcccg
    22021 cgctctgcgt ttcctgctgc aagaagtgct ggaaaaacag atgaccgcgc gtggttacat
    22081 ggttggtccg aatggcccgg ttaatctgca aatcatcgtt agccaactgt atgctgacgt
    22141 gtcccagggc aacgtgcgct acaacatcgc taccaaagcg gatatcgcca tcatcgctac
    22201 cgcgcagaat ggcaacaaaa tgaccaaaaa ctatcgtgcc agctacaacg ttgaaggtgc
    22261 gttccaggcc tccaacaaaa atatcgccga tgcggttaac agtgtgctga ccgataccat
    22321 cgctgatatg tctcaggaca ccagcattca cgaattcatc aagcagaacg cgcgttaatt
    22381 tctgccctct ggcccggtgc aagccgggcc tgtagacgcc catgtccagt caatatttac
    22441 gtatttttca acagccgcgt tcagccatat tgctgatcct gggttttgct tccgggctac
    22501 cgctcgccct gacatccggc accttacagg cctggatgac ggtcgaaaat atcgatctca
    22561 aaaccattgg tttcttctct ctggtaggcc aggcttacgt ttttaaattc ctctggtcac
    22621 cgctgatgga ccgctacacg cccccatttt ttgggcggcg gcgcggttgg ctgctcgcca
    22681 cgcaaatcct gttattagtc gccattgcgg cgatgggttt tctcgaacca ggcacccaac
    22741 tccgctggat ggcggcgctg gcggtggtga tcgctttttg ctctgcctcc caggatattg
    22801 tcttcgatgc gtggaaaacc gatgtgctcc cggcagaaga acgtggtgcg ggcgcggcaa
    22861 tcagcgtgct gggttaccgt ttagggatgc tggtttccgg cggcctagcc ctgtggctgg
    22921 cagataaatg gttgggctgg cagggcatgt actggttgat ggcggcgctg ttgatcccct
    22981 gtattatcgc gacgttgctt gcaccagaac caaccgacac cattcctgtg cccaaaacgc
    23041 tggaacaagc ggttgttgca cctctgcgag atttctttgg tcgcaataat gcctggctta
    23101 ttttgcttct tatcgtgctg tataagctgg gcgacgcatt cgccatgagc ctgacaacca
    23161 cgtttttgat tcgcggcgtc gggtttgatg cgggtgaagt aggcgtggta aacaaaacgc
    23221 ttggcttact ggcgaccatt gttggcgcat tgtacggtgg gattttgatg cagcgcctgt
    23281 cgctgttccg ggcgctgctg attttcggca ttttacaagg tgcgtctaac gctggttact
    23341 ggctgctgtc gattactgat aagcatctct acagcatggg cgcagccgtc tttttcgaaa
    23401 acctctgtgg cgggatgggc acatcagcct ttgtcgcgct gttaatgacg ctgtgtaata
    23461 agtcattttc cgctactcaa tttgccctgc tctcagcgct ttccgctgta gggcgagttt
    23521 atgtcggccc cgtggcgggt tggtttgttg aagcacacgg ctggtcgaca ttctatctat
    23581 tctccgtcgc cgctgccgta ccggggctta ttttgctgct ggtttgccgc cagacgcttg
    23641 aatatacacg agtaaatgac aactttatct cccgtaccga atatccggca ggttatgcct
    23701 ttgccatgtg gacactggcg gcgggcgtca gcctgttggc cgtgtggtta cttctgttga
    23761 cgatggacgc gctggatttg acgcacttct ctttcctgcc tgctctgctg gaagtcgggg
    23821 ttttagtcgc cctttctggc gtcgtgcttg gtggtttgct ggattatctg gcgctacgaa
    23881 aaacgcatct gacgtaatct gtaaatatta tttagctgta gtaatcactc gccgtaatta
    23941 ttacggctaa ataaacatca gcatcgctta ttaatatttg ttcttttgtg cggcttagcg
    24001 tttggaaatt attgacgcca tttataaatt ctatttttct tacacgattc agctaatgag
    24061 tctttatttt ctcaccaccc agttgtcact ttaatgataa ttatttgtta aataattgtt
    24121 ttatttcgca ttggttatac caattgccca ccaagactcc ttctgcactc cccttttgtt
    24181 a
//
LOCUS       DAJTRD010000058        24155 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTRD010000058 DAJTRD010000000
VERSION     DAJTRD010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24155
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.058"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..180
                     /gene="yjbD"
                     /locus_tag="OO315_002532"
     CDS             <1..180
                     /gene="yjbD"
                     /locus_tag="OO315_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5891399.1"
                     /translation="RVLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPE
                     ASFSWSANTSTRGRR"
     gene            complement(313..1185)
                     /gene="rluF"
                     /locus_tag="OO315_002533"
     CDS             complement(313..1185)
                     /gene="rluF"
                     /locus_tag="OO315_002533"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5891400.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1533..2480
                     /locus_tag="OO315_002534"
     CDS             1533..2480
                     /locus_tag="OO315_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5891401.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2550..3356
                     /locus_tag="OO315_002535"
     CDS             2550..3356
                     /locus_tag="OO315_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5891402.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3366..3773
                     /locus_tag="OO315_002536"
     CDS             3366..3773
                     /locus_tag="OO315_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5891403.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3773..4267
                     /locus_tag="OO315_002537"
     CDS             3773..4267
                     /locus_tag="OO315_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5891404.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4333..5130
                     /locus_tag="OO315_002538"
     CDS             4333..5130
                     /locus_tag="OO315_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5891405.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5141..5929
                     /locus_tag="OO315_002539"
     CDS             5141..5929
                     /locus_tag="OO315_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5891406.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5981..7210
                     /gene="sorE"
                     /locus_tag="OO315_002540"
     CDS             5981..7210
                     /gene="sorE"
                     /locus_tag="OO315_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5891407.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7592..8281
                     /gene="pepE"
                     /locus_tag="OO315_002541"
     CDS             7592..8281
                     /gene="pepE"
                     /locus_tag="OO315_002541"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5891408.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8372..10003)
                     /locus_tag="OO315_002542"
     CDS             complement(8372..10003)
                     /locus_tag="OO315_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5891409.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10223..13906)
                     /gene="metH"
                     /locus_tag="OO315_002543"
     CDS             complement(10223..13906)
                     /gene="metH"
                     /locus_tag="OO315_002543"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5891410.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14106..14930
                     /gene="iclR"
                     /locus_tag="OO315_002544"
     CDS             14106..14930
                     /gene="iclR"
                     /locus_tag="OO315_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5891411.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15247..17431
                     /gene="espL4"
                     /locus_tag="OO315_002545"
                     /pseudo
     CDS             15247..17431
                     /gene="espL4"
                     /locus_tag="OO315_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17400..19137)
                     /gene="aceK"
                     /locus_tag="OO315_002546"
                     /pseudo
     CDS             complement(17400..19137)
                     /gene="aceK"
                     /locus_tag="OO315_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19321..20625)
                     /gene="aceA"
                     /locus_tag="OO315_002547"
     CDS             complement(19321..20625)
                     /gene="aceA"
                     /locus_tag="OO315_002547"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5891412.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20655..22256)
                     /gene="aceB"
                     /locus_tag="OO315_002548"
                     /pseudo
     CDS             complement(20655..22256)
                     /gene="aceB"
                     /locus_tag="OO315_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22525..23454)
                     /gene="metA"
                     /locus_tag="OO315_002549"
     CDS             complement(22525..23454)
                     /gene="metA"
                     /locus_tag="OO315_002549"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5891413.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23611..24054
                     /locus_tag="OO315_002550"
     CDS             23611..24054
                     /locus_tag="OO315_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5891414.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5958 a         6170 c         6122 g         5905 t
ORIGIN      
        1 cgcgtactga ccaactccga aaccaacagc attaagaaag agtacatcga taaattaatg
       61 gtcgagcgtg aggctgaagc gaaaaaagcc cgccaattga agaaaaagca ggcttataaa
      121 ccggatcctg aagcgtcatt tagctggtca gcaaatacgt cgacgcgcgg caggcgctaa
      181 tgaacaatca acgcctgaga ttttcactcg tctgtgtgga cacaatcttc ccctcaccct
      241 aaccctctcc ccgaaggggc gaggggactg tccgagctct ttttggacgt tttcatcagt
      301 ctgaggcgtt aatcagcgtc ccttcttttt acgccccggc gaggtaaaac gcttaccgtt
      361 ggacgccggg cgaccgcctt tttccgccgt tttttccatc ttaacgactg gacgtttgat
      421 gcccgctgtt ttcggtttgg ccttcgcttt aggttttgcc tcggaagagg aattttcgat
      481 gagcttaaag aggtcgatta actcatcgtc ggttaaatcg cgccactccc ccagcggaat
      541 gccgcttaag ctgacgttca taatgcgcgt acgttccagc tttttcactt catagccgaa
      601 atgctcgcac atgcgccgga tctgacggtt cagcccctgc actagggtaa tgcggaagac
      661 aaacggcgct tcttttttga ctttgcactt tttcgtcacc gtcccgagga tcggcacccc
      721 cgcccccata ccacgaataa attcatcggt aatcggttta tcgaccgtca ccagatactc
      781 tttctcatga tcgttacccg cacggaggat cttattcacc agatcgccgt gattggtgag
      841 gaaaatcagc ccctgggagt ctttatccag gcggccaatc gggaacacgc gtttgctgtg
      901 gttaacgaaa tcgacaatgt tatcgcgctc gccatcttcg gtggtgctga caataccgac
      961 gggcttgttc agggcgataa gtaccaaatc ttcggcttcc cgaggctcaa tcaactgacc
     1021 gtttactttc acaatgtcgc cgggtttcac ctgatcgcca atggtggctc gcttgccatt
     1081 aaggaacaca ttgccttgct cgatatagcg atccgcttcg cggcgtgagc aaattccgct
     1141 ttcgctgatg tatttattta aacggactga tgagtcgggc agcataaatt ctcctgtaaa
     1201 acgcggaata taccgtacct cttagcgggg aaaaacaggg ttatgcggca acgtcgaaat
     1261 taaatgtgat ctgccacgcc ttttatcaca aaagtgtctc cggaagtacg atgcaaattt
     1321 gcctggcaaa ataaggataa tcaacatatt acgaatcgct gcacacctgt gctgatgact
     1381 actacgcaga gaaacaaatg tgcaagagaa agcgctattt ctccccatgc aaagagtgaa
     1441 aaatcgcttt gaaaggccct catgatgggg ttatatcaga tcaagcggtg agaaagcgta
     1501 tttgcacaaa tgtgcagaac caggaggcgg caatggaaaa cagtgacgat atccgtttga
     1561 ttgtgaagat tgcccaactc tattacgaac aggatatgac gcaggcgcaa atcgcgcgcg
     1621 aactgggtat ttaccgcacc accatcagcc gcttgcttaa acgaggccgc gatcagggaa
     1681 tggtcaccat cgccatcaac tatgactaca acgaaaatct ctggctggag cagcaactga
     1741 agcaaaagtt tggcctgaaa gacgttgtgg tggtgtcggg aaatgatgag gatgaagaga
     1801 ctcaactggc gatgatgggg ttacacggcg cgcaactgct ggatcgcttg ctggagcctg
     1861 gcgatattgt cggtttttcc tggggtcgcg cggtgagcgc actggttgaa aacttgccgc
     1921 aggcggggca atcgcggcag ttaatctgcg taccaattat tggcggaccg tccggtaaac
     1981 tcgaaagccg ctatcatgta aacacattaa cctacagcgc ggcagcgaag ctgaaagggg
     2041 aatcgcatct cgcggatttt ccggctctgc tggataaccc attaattcgt aatgggatca
     2101 tgcagtctca gcactttaaa accatctctg cctactggga taatctggat atcgccctgg
     2161 tgggaattgg ctcaccggcc attcgcgacg gcgctaactg gcatgcgttt tatggtggtg
     2221 aagagagtga cgacctgaat gcccgccagg ttgctggcga tatttgctcg cgcttttttg
     2281 atattcacgg cgcaatggtt gaaacgaata tgagcgaaaa aacgctctct atcgaaatga
     2341 ataaattaaa gcaggcgcga tattccattg gcattgccat gagtgaagaa aaatacagcg
     2401 gaattattgg tgcactgcgt ggaaaatata ttaattgtct ggtaacgaat agcagcacag
     2461 ctgaactatt actgaaataa aaatcttccc gatgtttacc gtaataaaaa ttacggcggg
     2521 tccctattat ctaaaatcag gagtctgtta tgcaaacgtg gttaaatttg caggataaaa
     2581 tcattattgt caccggcggc gcatccggta ttggtctggc gattgtagag gaattattag
     2641 cacaaggcgc gaatgtacag atggtcgata ttcacggtgg cgatggtcaa tatgaaggtc
     2701 ataaaggcta tcagttttgg ccgaccgata tttctagcac aaaagaggta aatcatacgg
     2761 tagcggaaat tatccagcgt tttggtcgca tcgacggtct ggtcaataac gccggggtca
     2821 atttcccgcg tctgctggtc gatgagaaag cgcctgccgg gcagtatgaa ctcaacgaag
     2881 ctgcattcga aaaaatggtc aatatcaacc agaaaggtgt ttttctgatg tcgcaggcgg
     2941 tggcgcgaca gatggtcaaa caacatgatg gcgtgattgt gaatgtttcc tcagaaagtg
     3001 ggctggaagg ctcagaagga caaagctgtt acgccgctac caaagccgcg ctcaatagct
     3061 tcacgcgctc ctggtcgaaa gagctgggta aacacggtat ccgtgtggtc ggtatcgcgc
     3121 ccgggattct ggaaaaaacc gggctacgca cgccggaata tgaagaggcg ctggcgtgga
     3181 cgcgcaatat caccgtcgag cagctgcgtg aaggctatac caaaaacgcc attcctattg
     3241 ggcgcgccgg aagattagca gaagtagctg attttgtttg ttatctgttg tctgaacgcg
     3301 ccagctacat caccggagta accactaaca ttgcgggcgg caaaacgcgc ggctaaggag
     3361 gctttatggt caacgccatt ttttgtgccc acggcaaatt agcttgcgcc atgctggaat
     3421 cggttcagat ggtttacggc aacgccaatg ttgaagccgt tgcatttgtg cccggtgaaa
     3481 acgccggaga cattgcggta aagctggaaa agttagtaag cattcacaat caagatgagt
     3541 ggctgattgc cgtggattta cagtgcggca gcccgtggaa tgcggcagca atgcttgcga
     3601 tgcgcaatcc tcgtttgcgg gtaatcagcg gcctttctct gccgctggcg ctcgaactgg
     3661 ttgataacca ggacagcatg aatgttgatg aactgtgtga acacctgacg caaatcgcaa
     3721 agcaaacctg cgttgtctgg aaacaactgg caacgacaga ggaggatttc tgatgaacat
     3781 tacgctcgcc cgcattgatg accgtctgat ccacggccag gtcactaccg tctggtcgaa
     3841 agtggctaac gcccagcgca ttattatctg caatgacgaa gtttataacg atgaagtgcg
     3901 ccggacatta ttgcgtcagg ctgcgccacc gggaatgaag gttaacgtgg tcaatattga
     3961 aaaagccgtc gcggtttatc acaacccgca atatcaggac gaaaccgttt tttatctttt
     4021 cactcgccca caggatgcct tagcgatggt tcgccagggg gtaaaaatag gcaccctgaa
     4081 tattggcggc atggcctggc ggcccggtaa aaaacagtta accaaagcgg tttcattaga
     4141 tgatgatgat attaacgcat ttcatgagtt aaataatctt ggtgtcatac tggatttacg
     4201 cgtcgtagcg tcagatccct caatcaatat tatcgacaaa ataaacgaac agttaatcgc
     4261 aaattaaata atagcgcctg tgtacatatg ctttaacagg cagggatgac gacacttaaa
     4321 aggtgacaaa tcatggaaat aagtacccta caaatcattg ctatatttct tttttcctgt
     4381 attgccggaa tgggcagcgt gctggatgaa tttcagactc atcgcccgtt aattgcctgt
     4441 acggtgattg gtttaattct cggtgattta aaaaccggaa taatactcgg cggtacgctg
     4501 gaattgatag ctctctgctg gatgaacgtc ggcgcggcgc aatctccgga ttctgcactc
     4561 gccagcataa tctccgccat tctggttatc gttggtcagc aaagcatcgc caccggaatc
     4621 gccatcgcgt tgcctgtggc tgcggcaggc caggtgctga cggtgtttgc ccgtaccatc
     4681 accgtagtgt tccagcacgc ggcggataaa gcagcagaag aggcgcggtt tcgcactctc
     4741 gatattctgc atgtctccgc gcttggcgtg caggcgctgc gcgtcgccat tccggcactg
     4801 attgtctcac tgttcgtcag cgccgatatg gtcagcaata tgttaaacgc cattcccgaa
     4861 tttgtgaccc gaggactaca gattgctggc ggttttatcg tggtggtcgg ttacgccatg
     4921 gtgctacgta tgatgggcgt gaaatatttg atgcctttct ttttcctcgg tttcctcgca
     4981 ggtggctacc tcgatctcag cctgctggcg ttcggtggcg tcggcgtgat catggccctg
     5041 gtctacatcc agctaaatcc acagtggtgt aaagctgaac ctcaactcca ggccaccgcc
     5101 tccaccgccc ttgaccaact tgacgattaa cggagccatc atggaacaga gaaaaattac
     5161 acgcagcgat ctggtgagca tgtttctgcg ctccaacctg caacaggcgt cctttaactt
     5221 tgaacgtatt cacgggctgg gcttttgcta cgacatgatc cccgccatca agcgacttta
     5281 cccattaaaa gaggatcagg ttgcggcgct caggctacac ctggtgttct tcaataccac
     5341 gccagccgta tgtggcccgg tcatcggcgc ggaaattgat gacggtacca tcaacggcat
     5401 caaagtcggt ctgatgggac cattggcagg agttggcgat ccactggtct ggggaacgct
     5461 gcgcccgatt accgccgcgc tcggcgcatc tctggcactt tcgggcaaca ttctcggccc
     5521 gctgctgttc ttctttattt tcaacgcggt gcgtctggcg atgaagtggt atggcctaca
     5581 gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg ggcgggaatg tgctgcaaaa
     5641 actcaccgaa ggcgcgtcga ttctcggact gtttgtgatg ggcgtgctgg tgaccaaatg
     5701 gacgtcaatc aacgtaccgt tggtggtttc acaaacgcat gccgccgatg gctccaccgt
     5761 caccatgacc gtgcagaaca ttctcgacca actttgccct ggtttgctgg cgctcggtct
     5821 gacgctacta atggttcgtc tgctcaacaa aaaaattaac ccggtatggc tgattttcgc
     5881 cctgtttggc ttagggatta tcggcaatgc tctgggcttc ctgtcctgat tcttcgcccc
     5941 ggcacgactg ccgaggccat cgctcaacat gaggtggttt atgaaaacaa cagctctgcg
     6001 tctttatggt aaacgtgatt tacgcctgga aacctttgac cttcctgaaa tgcaggagga
     6061 tgaaatcctc gcaacggtgg tcactgacag cctgtgcctc tcttcctgga aagaggccaa
     6121 tctgggtgaa aaccataaaa aagtacccga cgatgtgacg accaacccaa tcatcatcgg
     6181 ccacgaattt tgcggcgata ttctggccgt gggtaaaaag tggcagcaca aattccagcc
     6241 gggtcagcgt tatgtgattc aggccaacct gcaactcccc gaccgcccgg attgccccgg
     6301 ctactccttc ccgtgggtag acggcgaagc gacgcatgtg gttattccca acgaggtcat
     6361 ggaacaatat tgcctgctgg catacgacgg cgaaacctat tttgaaggct cactggttga
     6421 accgctttcc tgcgtgattg gcgcgttcaa cgccaactat catcttcagg aaggtagtta
     6481 taaccacacg atggggattc gcccgcaagg gcacacgctg atcctcggcg gcaccggacc
     6541 aatgggactg ttggcgattg attatgcgct gcatggacca gttaacccgt cgctgctcgt
     6601 cattaccgat accgacaacg acaaattgag ttatgcgcgc aagcactatc catcagaacc
     6661 gcaaacactg attcattatc tcaatgccac cgatgcagca tttgatacgt taatggcgct
     6721 gagtggcggt cacggcttcg atgatatttt cgtctttgtg cctaatgaag gactggtgac
     6781 tctcgcctct tccttgctgg cgacagatgg ttgcctgaat ttcttcgccg gaccgcagga
     6841 taaacatttc agcgcgccaa ttaatttcta cgatgtgcat tatgcattta cccactacgt
     6901 gggcacgtca ggcggcaata ccgacgacat gcgcgcggcg gtcaaactga tcgaagagaa
     6961 aaaagtgcag gccgcaaaag tggtaacaca tattcttggg ctgaatgccg cgggcgaaac
     7021 cacgcttgaa ttgcctgccg tcggcggcgg caaaaagctg gtgtataccg ggaaatacct
     7081 gccgctgacg tcactcacgc agattcagga tcaagcactg gcggcgattc tggcgcgtca
     7141 tcaggggatc tggtccggtg aggtggagca atacctgctc gcccatgcag aggcaatttc
     7201 ccatgattaa tcgcgctact taactctgca tgtcgctggc tggtcgcccc agcaacttcg
     7261 cgaccggatg tgcggctgat tgctgaagca gcacaatttg cacgagaagg ataaaaaata
     7321 tcagcgcctt aaggggcgct caaattggtg gcaaagtaca cattgtttat gccggatgcg
     7381 gaatgaagcc ttattcggtc tacataattt tgaaaatcca atatattgca gttatctggt
     7441 aggccaggta agcgtagcgc atcaggcaat gctgtgtttg tcatcagtct cagcgcccgg
     7501 tgcaatacgt gacatcgtga gccatgtcag taagtggtta aattcgcagt ggtagcatgc
     7561 cttccgtgat ttcttactaa ccggagaagt aatggaactg cttttattga gtaactcgac
     7621 gctgccgggt aaagcctggc tggaacatgc actgccgcta attgctgaac agttgcaggg
     7681 tcgccgctca gcggtgttta tccctttcgc tggcgtaacg cagacctggg atgattacac
     7741 agagaaaacg gctgcggttc tcgctccgct gggtgtttct gtcaccggta ttcatagcgt
     7801 tgtcgatccc gttgccgcga ttgaaaatgc ggaaattgtg attgtcggcg gcggtaatac
     7861 tttccagttg ctgaaacagt gccgcgagcg cgggctgctg gctccaatta ctgacgtggt
     7921 taaacgtggc gcgctgtata ttggctggag cgcaggcgct aaccttgctt gcccaaccat
     7981 tcgtaccacc aacgatatgc cgattgtcga tccgcaaggt ttcgatgcgc taaatctgtt
     8041 cccgttgcaa atcaacccgc acttcaccaa cgcgctgccg gaaggccata aaggtgaaac
     8101 ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca gaactgacga ttattggtct
     8161 gccggaaggt aactggatca cagtgagtaa aggtcacgct acgctgggtg gcccgaacac
     8221 cacttatgtg tttaaggctg gtgaagaagc ggttccgctg gaagctggtc accgttttta
     8281 agtgaattgc cggatgcatg atccggcatc ttgttcatag tgcctgatgc gacgcttgac
     8341 gcgtcttatc aggcctacag gattcacaat attaatactc gtcccgtcct tcatcttcat
     8401 ccggctgttc cagcacactg taagccaccg aacaaaacag cgagttcagg cgctgcatat
     8461 ctcccagtaa gcctaaatgt agcgaactgg tttcaatgct ctgcacgttt tgctgatgca
     8521 ggcgatcgac gtgggcgtgg gaatagcggc gattaagaat gcgaaaacga tgtttgctgc
     8581 gacgcaaacg acgagcgctg gtgacatcgc cagagaagaa caccgacatt gccagcttta
     8641 aattactgag caattgctca tagagcgcat ccagttcctt caacccatca agcgaaaatg
     8701 cccgccgtgc tgccagcgat ttatcggcaa tttcgctgcc catgcgctcg acgatatcgg
     8761 aggcctgttc aaggttgagc gacatttcga tgatctccgc ccagcggcgc gactcttcct
     8821 ccgccaactc ttcttttggc atccgcgcca gatacagctt aatggcggtg tagagaacgt
     8881 tgatatcatc tgccagctta cgcagctctt tctcctgccg tggctcgccg tgcatcactt
     8941 tattcagccc ttccatcatc tgttccatgg cgtcgccaat gcgcagggtt tcgcgcgcgg
     9001 cgttcgccag agcaagcgtg ggcgtgtcca gcgcgctgac atccagatgt ttaggccgta
     9061 gctgggtatc cagttccggt tcatcgcgaa taatcgtttt gcaaaaccgt gccatcgggt
     9121 caacaaatgg cagcatgacc aggcaacgta caaggttgta gaagacgtgg aaatagatca
     9181 ccagttccgc ttttggcaat gacagcttcc ccattgtctc tgccagcaaa tggacaaacg
     9241 gcaggataat caggctaccc accagcttaa acagcagact acccagcgcg acacggcggg
     9301 ctgcggcatt ggcggcactg ttgttgagca tcgccagcag gccggaaccg aggttagctc
     9361 caatcaccag acagagcgcc acggggaagg agataatgcc tgcagcggtc agagtcgcgg
     9421 tcagcagtac agcagcaagg ctggagtagc tgataatggc gaacatcgcg ccaatcagcg
     9481 catccagcag aatatcgccg gtcagcgagg caaagatcac ctgaacgccg tttgcctggg
     9541 tgatcggcgt tacggcctgc acaatcaact ccagcgccag caaaatcagc ccaagaccaa
     9601 taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg tccgaggaaa aaaatcacgc
     9661 cgataaaaat aagtaacggt gacagccagg ataagtcgaa ggtgagaata cgcgccatta
     9721 gcgccgtccc gacatctgcc cccagcacaa tgaccagagc cggtgcgagg gctaccagat
     9781 cctgagcgac gaacgaggtc accagcatgg tggtggcatt actgctctgt accagtgcgg
     9841 taacgccgat ccccgcgcaa aaggcgagcg gctttttttc gacgctccgg ctaaggacag
     9901 tacgcaaacg cgcgccgaag acgcgcatta cgccggttcg aacaatatga gtcccccaga
     9961 ccagcagggc gacggcagaa agcaggtgaa gcagcgttaa cacgagttca gattctcctt
    10021 atcgttatat gttcctgaag caattgaatg accaaaggac cggaaacacg ctgactggca
    10081 tctttactgt gccttttgac attcgttttt ccggctaaga ttattccagc ctgtttccag
    10141 tataagtgtt tcatattaat aaagagacag ggcactcatt gagcgccctg tttgttgtaa
    10201 ggaaaagtga cagatttgtg aatcagtccg cgtcataccc cagattcggt gccagccagc
    10261 gctcaacatc ggagacgctc atacctttac ggcgggcatg gtcttcaacc tgatcgcgct
    10321 gaatttgcgc caccgcgtag tacttgctgt ccggatgact gaaataccaa cccgaaaccg
    10381 atgcaccagg ccacatcgcg aaagattctg tgagtttcat gccagtatgt ttttccactt
    10441 ccagcagctc ccagatggtg gctttttccg tatgttccgg gcaggccgga tagcccggtg
    10501 ccggacggat gccctggtag ttttcgcgga tcagttcttc gttgctgagg ttctcgttcg
    10561 gcgcatagcc ccagtagact ttacgcacac gctcatggag atactccgca aaggcttcgg
    10621 ctaaacggtc ggcaagcgct ttcaccatga ttttgttgta atcatcgtgc tgcgcttcaa
    10681 aggcatcagc cagtgcgtcc tcttccagcc cgccagtcac ggcaaatgcg ccgatgtaat
    10741 ctgctttacc agaaagcttc ggcgcaacga agtcagcgag acagtagtta gcgaatcccg
    10801 ttttttcggt ctgttgacgc agatggtggc tgacgttgat cacatgggtg cgcgtttcgt
    10861 cacggtagat ttcaatgtca tcgcccacac ggtttgccgg gaacaggccc accacgccac
    10921 gcggattcag cgttttctcg gcgcttaatt tatccagcat gtcgttggcg tctttaaaca
    10981 gccgctgcgc ctcaacgccc accacttcat cttccagaat gcgcggatac ttcccggcca
    11041 gcgaccaggt cataaagaac ggtgtccagt cgatgtaatt acgcagcgtt tcgatgctgg
    11101 cttccacttc ctgcacgccg agacggtgcg ccaccggcgg cgtgtaagcc tgccagtcaa
    11161 atgcgaaatc gttatcgcgc gccgcttcca gcgtgaccgg tggtgtgcgt ggtttcttgc
    11221 gcccgtgctg aatacgcacg gtttcgtact ccttgcaggt acgagcgaca aaatcatcac
    11281 gctgagtatc ggaaagcagc gccgctacca caccaacggt gcgcgaggca ttctgcacat
    11341 acaccgtagg gccgctgtag ttctgctcga ttttcaccgc cgtgtgcgct tttgaggtgg
    11401 tcgcgccgcc aatcagtaac ggaatggcga agccctgacg ctccatctct ttcgccacgt
    11461 taaccatctc gtccagcgac ggcgtgataa gccccgaaag gccaatcaaa tcagcattca
    11521 cttctttggc gatacggaga attttttccg caggcaccat aacgccgaga tcgacaattt
    11581 cgtagttgtt acattgcagc accacaccaa cgatattttt accgatgtcg tggacatcgc
    11641 ctttcacggt ggcgatcacc atcttgccgt tggttttacc ctgctctttg ctggcttcaa
    11701 taaacggttc gagatacgcc actgcctgtt tcatgacgcg cgccgatttg accacctgtg
    11761 gcaggaacat tttgccttcg ccaaacagat cgccgaccac gttcatgccg tccatcaacg
    11821 ggccttcaat cacttcaatc gggcgcgtag cctgctggcg ggcttcttca gtatcctgct
    11881 cgataaactc ggttatgcct ttgaccagcg agtattccag acgtttattc acgtcccacg
    11941 agcgccactc cgcttgctgg gcgttagcag tgtcgtccgc tttactgccg cgatatttct
    12001 cggcaagctc cagtaaacgc tcagtgccat cgtcacgacg attaagaatc acatcttcca
    12061 ccgcgtcgcg cagttcagcg ggtaggtcgt cgtaaatcgc cagttgcccg gcgttgacga
    12121 tccccatatc catgccattg cgaatagcgt agtagaggaa cactgcgtga atggcttcgc
    12181 gcaccggatc gttgccacgg aatgagaaag aaacgttaga tacgccgccg gaaatcagcg
    12241 cgtgcggcag ttcgcgtttg atgtcttcac acgcgccgat aaagtcctgc gcgtagttgt
    12301 tgtgctcttc aataccggtg gcgaccgcga agatgttcgg gtcgaagatg atatcttctg
    12361 gcgggaagcc aacctcttcg gtgaggattt tgtacgcccg acggcaaatc tcgattttcc
    12421 gtgcgcgagt atcggcctgt ccctgttcgt caaaggccat caccaccact gccgcaccgt
    12481 agcgacgcaa caatttcgcg tgatggataa aggcgtcgac gccctctttc atcgagatag
    12541 agttaacaat gcctttgccc tggatacact tcagaccttt ttcaatgaca tcccattttg
    12601 aggagtcgat cataatcggc acgcgagcga tatccggttc accggcaatc agattgagaa
    12661 aacgcaccat cgccgcttcg gcatcgagca tcccttcatc catgttgata tcgataatct
    12721 gcgcgccgtt ttccacctgc tgacgcgcca catccagcgc ctcgctgtat ttctcttctt
    12781 tgatcaggcg cttgaactta gcagagccgg tgacgttggt gcgttcaccc acgttcacaa
    12841 acaggctatc gtcgccaatg ttcagcggct ccaggccaga caaacggcag gctacgggaa
    12901 tttccggcag tttgcgcggc gctaatcctt ctactgcacg actcatcgct gcaatatgtt
    12961 gcggcgtggt cccgcagcaa ccaccgacga tattgagaaa gcccgcttgc gcccattcac
    13021 gtatctgttt tgccatcgtg tcggcgtcga gatcgtattc accaaaggcg ttaggcagcc
    13081 cggcgttcgg gtgggcggtg acgtagcatt ccgcaatccg tgatagttcc tgcacgtact
    13141 ggcgcagttc atcgggcccc agcgcacagt tcaggccaaa ggtcagcgct tcggcgtggc
    13201 gcaatgagtt gtaaaatgct tcggtggtct gcccggagag cgtgcgcccg gaggcgtcgg
    13261 tgatggtgcc ggagatcata atcggcagct caacgcccag cgcttcaaac tccgttttca
    13321 ccgcaaagac cgccgctttg gcgttaaggg tgtcgaaaac ggtttcaatc aggatcagat
    13381 ccgcaccacc ttccaccagc gctttggtgg attcgcgata ggccgccgcc agctggtcaa
    13441 aagtgatgtt gcggaatgcc ggatcgttga cgtccggaga aatagacgcc gtgcggttgg
    13501 ttgggccaag aacaccggca acgtagcgcg gtttctccgg cgtgcgcgcg gtccactcgt
    13561 cagcacaggc gcgcgccagt ttcgccgccg caaagttgat ttccgccgac agggattcca
    13621 tctggtaatc cgccatcgca atggtcgtgg agttgaaggt gttggtttcg atgatatccg
    13681 cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac ttccggttta ctgagtacca
    13741 gcaggtcgtt gttgcctttg aggtcgcatg gccagtcggc aaagcgttca ccacgaaaat
    13801 cggcttcgtt cagtcgatag ctctggatca tggtgcccat accgccgtcc aacaccagaa
    13861 tacgttcatt taactgcgca cgcagttgtt ccactttgct gctcacactt gctcccgaca
    13921 cgcttaaccc agatttaata aaaattcaac aaaccatact ggaataaacg catctgtggt
    13981 aaaagcgacc accacgcaac atgagatttg ttcaacatta actcatcgga tcagttcagc
    14041 aactattgca ttagctaaca ataaaaatga aaataatttc cacgatacag aaaaaggagg
    14101 ctgtcatggt cgcacccatt cccgcgaaac gcggcagaaa acccgccgtt gccaccgcac
    14161 cagcgactgg acaggttcag tctttaacgc gtggcctgaa attactggag tggattgccg
    14221 aatccaatgg cagcgtggca ctcacagagc tggcgcaaca agccgggtta cccaattcca
    14281 cgacccaccg cctgctaacc acgatgcaac agcagggttt cgtgcgtcag gtcggcgaac
    14341 tgggacactg ggcaatcggc gcacatgcct ttatggtcgg cagcagcttt ctccagagcc
    14401 gtaatttgtt agcaattgtt caccctatcc tgcgcaatct aatggaagag tctggcgaaa
    14461 cggtcaatat ggcggtgctt gatcaaagcg atcacgaagc gattattatc gaccaggtac
    14521 agtgtaccca cctgatgcga atgtccgcgc ctatcggcgg taaattgccg atgcacgctt
    14581 ccggtgcggg taaagccttt ttagcccaac tgagcgaaga acaggtgacg aagctgctgc
    14641 accgcaaagg gttacatgcc tatacccacg caactctggt gtctcctgtg catttaaaag
    14701 aagatctcgc ccaaacgcgc aaacggggtt attcatttga cgatgaggaa catgcgctgg
    14761 ggctacgttg ccttgcagcg tgtattttcg atgagcaccg cgaaccgttt gccgcaattt
    14821 ctatttccgg accgatttca cgtattaccg atgatcgcgt gaccgagttt ggcgcgatgg
    14881 tgattaaagc ggcgaaggaa gtgacgctgg cgtacggtgg aatgcgctga ctttttctgg
    14941 cgggcagagg caatattctg cccatcatac ctgagtggca atagaataag ggtgtctgtt
    15001 aatcgcattg acgccaaaat aacttaatgt catacacttc acacactttt attaactcag
    15061 cattattttt aaacatcaaa ccacttaatt attaacaaaa tcgcaagatc tcattccacc
    15121 gaaactttta cggctgcatt atgagattga gcgatttatg atattttttt agttgtaatc
    15181 atattaaatt ttttcactgt tctgatagtt aaaattcaag acatcaataa acaatgagat
    15241 atttaaatga ttactcgtat tcctcgtagt tctttctctg caaatattaa taatacagcc
    15301 cagacaaatg aacaccaaac cctgagtgaa ttgttttata aagaactcga ggataaattt
    15361 tctggcaagg agctggcgac gcctctatta aaaagcttct cagagaactg tagacaaaat
    15421 gttcgtcata tctttagcaa caaggatttt gtcattaaat tttccacgtc cgtcttacaa
    15481 gctgataaga aagaaattac gatgattaat aaaaacgaaa acacgacact cactcaaacc
    15541 attgctacaa tatttgaaga atacctaatg gaaattttac ctcaacgctc agacactctt
    15601 gataaacaag aattaaacca aaaatcagat agaaaagaaa aagaattccc aagaattaaa
    15661 cttaatggtc aatgttattt cccggggcgc cccaaaaccg tatagtatac cgacacattg
    15721 ctgcacaata tattaatgat atttatcaga atgttgatta caaaccccat caagatgatt
    15781 actcttcagc tgaaaaattt ctcactcact tcaacaaaaa atgcaaaaac cagactttgg
    15841 cgttgatttc cagccgtcct gaggggcgtt gcgttgctgc ctgcggtgat ttcgggctag
    15901 ttatgaaagc atattttgac aagatggaat caaatggcat cagtgttatg gcagccatat
    15961 tactggtgga taaccatgct ttgacggtcc ggctaagaat aaagaacaca actgaaggat
    16021 gtacccatta cgtggtttcg gtttatgatc ctaatgtaac taacgataaa ataagaatta
    16081 tgagcgaaag caaagaggat attaaacact attctctgat ggattttatg aatgtagatt
    16141 atagcctcct gaaatggtca aatgatcatg ttattaacca atctgttgca ataattccag
    16201 cacttccgaa agaacagcta ttgatgttaa aaggaactgt ggatgaaata acccctccat
    16261 tatcaccagc aacgatgaat ttgctaatgg caattggtca gaatcaccaa cttacgcaac
    16321 tgatgattca gctccagaaa atgccagaac tacatagaac agaaatgttg actgcctata
    16381 atagtattaa cctccccggt ttatatttgg ctataaatta tggcaatgcg gatatcgttg
    16441 agactatttt caattcattg tcagaaacag gatatgaagg gttactctcg aaaaaaaatc
    16501 tcatgcatat tctggaggca aaagataaaa atggtttttc tggattattt ttagcgatat
    16561 cacgtaagga taaaaatgtt gtaacctcga ttctgaacgt cttacctaaa ctggccgcaa
    16621 cacatcattt agataatgaa caggtgtata aattcctgag tgccaaaaat agaacgtcca
    16681 gccatgtttt ataccatgtt atggcgaatg gtgatgccga catgctgaaa atttttttgg
    16741 tcgcgttacc tttgttaatt cgcacatgtc atttgactaa agaacaggta ctcgatctcc
    16801 tgaaggcaaa ggatttttat ggttgcccaa gactatacct ggcgatgcaa aatggacata
    16861 gcgatatcgt gaaagttatt ctcgaagcat tgccctgtct ggcccaggaa attaacattt
    16921 cagcttccga tattgtagat cttctgaccg ctaaaagtct tgcgcgcgac acgggtttgt
    16981 ttatggccat gcagcgcgga catatgaacg ttattaatac tatttttaac gcattaccca
    17041 ctctttttaa tacgtttaaa ttcgataaaa aaatatgaag cccctcctcc tggcaaataa
    17101 ttctaatgaa tatcccggtt tgttttcagc gatacagcat aaacaacaaa acgttgtaga
    17161 gacggtttat cttgctttat ctgaccatgc acgcctgttt ggatttaccg ctgaagatat
    17221 tatggatttt tggcaacaca aagcgccaca aaaatactct gcctttgagt tggcttttga
    17281 atttggtcac cgggttattg ctgaattaat ccttaataca ttaaataaga tggctgaaag
    17341 ctttggcttt acggataacc ctcgatacat tgcggagaaa aattatatgg aagctttact
    17401 caaaaaagca tctccccata ccgtacgcta aatctttgcc tgcgccgata cgcataaaca
    17461 tcttccacat gcccttcacg tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac
    17521 aggtcggcgt gcatctcttc aaacagcgga ccaatacgcg ggtcggcgca tagccagtgg
    17581 cgaaactctt ccgggaaaac atcgcccggc gagacgctgt accacggttc gctggcaagt
    17641 tcgtcttccg gatagcgcgg cggcgggatg tcgcggaaat tcacttccgt catgtagcaa
    17701 atttcatcgt aatcataaaa aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac
    17761 agcatgtcgc cagggaaaat gttagcagcg gcaagctggc gaatagcgtt accgtattct
    17821 tcaatggcgt cgcgcaactg ctgaccttcc acttgttcca gccagatatt gagcggcacc
    17881 atccgccgct cgattataga gatggcgaat tacaatctgc tcaccaagat cggtgatttt
    17941 ttccgctgct tcctggagca gtaattccat taataccggg gaaatatgcc gcttctccag
    18001 cacaaagttt tcaaactcct gggtgtccgc cattcggccc acacggtcgt gctctttcac
    18061 cagttgatag caggcacgaa cgtgagcggc agacatctct ttctgcggcg cgaacttgtc
    18121 tttgatgact ttgaataccc gatcaaagcc cggcagcgta aacaccaaca tcaccattcc
    18181 acgaataccc ggcgcttcaa tgaactgctc attacagccc tgtagataaa cgagatattc
    18241 gcggtagctt tcggttttgg cgtgcttctg gcagccgata gccatataca attcagcagt
    18301 ggttttacct ggcagaattt cccgtagcca ctcgaccagc gctgcgggca gcggtgcata
    18361 aaccataaaa taagaacgcg caaagccaaa aacaatgctc gcttcggcgg tcgtcgtcag
    18421 gcaggtatca ataaataact cgccgtcgtc cgtctggtgg atcggcagta agaagggcaa
    18481 cgtgccggaa ggcgtgatca gtttgcccac cagccaggcg gctttattgc ggtaaaacag
    18541 ttcgttcgcc acctgtaaat gactttccgc gaggttgtct gtccccagcg tttccgtcag
    18601 atggcgaatg atgtaatgaa tgtcacggct tttattctgc cagcgcaggc gcagcggcag
    18661 gtcgctgata acgcgcatca gtagagattc ccagccgtga tcggggtgaa agtctttcgc
    18721 cagcgggcgg ggaatggtac gaaagcggcg ctctggctga gagctaaaga taaaaagccg
    18781 ctcgggagta agcgagcggt ggtcaaataa ccgacagtac acggagttaa aaaagctctc
    18841 cgcaatctcg aagcgcgggt aatccggcaa cagccgcgtg taatgctctt taacgcgcag
    18901 taaaaatgcc gcgtccgtac tttggccgtt agtaatgcag cgcagttgct ccacgaccag
    18961 accaacgtga tgatcgtaaa gatggatacg gtttttcatc gcctgctgga cagcatgcca
    19021 gtcggcctgt tcgaaacgct gctgcgcccc ggaagtcact tcgaggaatc gaccatactg
    19081 agcgtcgaag ccttgcaaaa tggtttgagc aatcagtaat tccaggccac gcggcattta
    19141 gcgccctcat caggagcaga gaattgccgg atgcggcgta aacgccttat ccagcctacg
    19201 ttcggcaacg gttgtaggca tgataagacg cgcaagcgtc gcatcaggca tcgtgcaccg
    19261 attgccggat gcggcgtaaa tgccttatcc ggcctacagt cagcaacgtt tgtaggttgc
    19321 ttagaactgc gattcttcag tggagccagt cagcgcggtg actgaagacg tgccgccctg
    19381 aataatagtc gtcactttat cgaagtaacc tgtacccact tcctgctggt gagatacgaa
    19441 ggtatagcca tctttcgcgg cggcaaattc cggctgctgc actttctcaa catagtgctt
    19501 cataccttcg ccctgcgcat aagcgtttgc caggtcgaac atgttaaacc acatgctgtg
    19561 gatacctgct agggtgatga actggaactt gtagcccata tccgacagct gctgctggaa
    19621 gctggcaata gttttgtcgt cgaggttttt ctgccagttg aacgacggcg agcagttata
    19681 agccagcagt ttgcccggat atttcgcgtg gatagcttgt gcaaagcgac gcgccagttc
    19741 cagatccggc gtggaggttt cacaccagac caggtcagca tatggcgcat acgccaggcc
    19801 acggctgatc gcttgctcaa tgcccgcatg agtacggaag aagccttcac tggtacgctc
    19861 gccggtaata aattcgctgt catacgggtc gcaatctgag gtgatcagat ccgccgcatc
    19921 agcatcggta cgtgcaacca gcagggttgg aacgcccgtc acgtcagctg ccagacgcgc
    19981 cgcgaccagt ttctgaatag cttcctgagt tggcactaaa actttgccgc ccatgtgacc
    20041 gcatttcttc actgacgcca gctgatcttc gaagtgaact gccgcagcac cggcttcaat
    20101 catcgctttc atcagttcaa aggcattcag gacaccgcca aaaccggctt ccgcatcggc
    20161 aacgatcggc aggaagtaat cgacatagcg cggatcgccc ggctcaatgc ccacggacca
    20221 ttggatctga tcggcacgac ggaaggtgtt gttgatccgc tccaccacag ctggcaccga
    20281 gtttgccgga tagagcgact gatccggata catgctggcc gccaggttag cgtccgccgc
    20341 tacctgccat cccgacagat agactgcttc aatacccgct ttcgcctgtt gcagcgcctg
    20401 accgccagtc agtgcaccga ggttgttgat gtagcctttt ttcgactcac cgtgcagcag
    20461 acgccacatt ttcgctgcgc ccagttgcgc cagcgtgcat tcaggattga ctgaaccgcg
    20521 taatttcacc acatcttccg cactgtatgg gcgagtaatg ccttcccaac gcggttgagt
    20581 ccactctttc tgtaattctt caatttgttg tgtacgggtt ttcatgtgca gatgctccat
    20641 agttatgtgg tggtttacgc taacaggcgg tagcctggca gggtcaggaa atcaattaac
    20701 tcatcggaag tggtgatctg ttccatcaag cgtgcggcat cgtcaaaacg gccctgggag
    20761 aaacgttctt cgcccagttc gctggcaatg actttcatct cttcgcccag catctggcgg
    20821 aacaaggctt tggtcaccgg tttgccattg ctcaacgttt tttgatgatg gatccactgc
    20881 cagatcgagg tacgggaaat ttcagccgtc gccgcatctt ccatcaggcc ataaatcggc
    20941 acgcagccgt tgccggagat ccacgcttcg atgtactgca cagccacgcg aatgttagcg
    21001 cgcatacctt cttcggtgcg ttcaccatca caaggtgcca gcagctgatc ggcagtaatc
    21061 ggcgcgtctt gttcgcgcaa tacttcaagc tgatttttac gggagccgag aatgtcgttg
    21121 aataccgcca ttgccgtgtc cgcaaggcct gggtgagcga tccatgtgcc atcgtgaccg
    21181 ttattggctt ccagcgcttt atccgctttt actttgttga gcacctggtt attacgctct
    21241 tcatctttgc tcggaataaa cgcggccatg ccgcccatcg caaaagcacc gcgtttatgg
    21301 caggttttaa tcagcaggcg tgagtaagca ttcaggaatg gtttatccat cgtcacggcc
    21361 tgtctgtctg gcaggacacg atcgggatag tttttcaacg ttttgatata gctgaagatg
    21421 taatcccagc gaccgcagtt cagaccaaca atatggtcgc gcagcgcgtg aaggatttcg
    21481 tccatctgga acacggcggg cagcgtttca atcagcaacg tcgccttgat ggtgccgcgc
    21541 ggcagattaa agcgatcttc tgcatagctg aagacttcgc tccaccaggc cgcttcctgc
    21601 taggactggg ttttcggcag atagaaatag ggaccgctgc cctttgccaa cagtgcctga
    21661 tagttgtgga agaaatagag cgcaaaatca aacaggctac cggggattgc ctcaccacgc
    21721 caggtgacat gtttttccgg caagtgcaga ccgcgtaccc gacaaatcaa aaccgctgga
    21781 ttgggcttga gctgataaat tttgcctgct tcattggtat agctgatggt accgttaacc
    21841 gcatcgcgca ggttaatttg cccgtcgatc actttgttcc agtctggtgc cagtgaatct
    21901 tcgaaatcgg ccataaagac tttcacattg gcgttcagtg cgttgatcac catcttgcgc
    21961 tctaccgggc cagttatctc tacgcggcgg tcttctaagt ccgcaggaat cccgcgaatt
    22021 ttccaatcag catcgcgaat ggaagccgtt tccgaaataa aatcaggcaa cgttccgtta
    22081 tcaatatctt gctgctgctg aatgcgcgct gccagaagtt tattgcgttg tggcgtaaaa
    22141 tgcgtcacca gctcagtcag aaattctacc gcttcggcag taagaatttg cttctcctgc
    22201 tcgccatacg gccttgtgaa agccagttca tcggttgttg ttgcctgttc agtcatcgtg
    22261 cagctcctcg tcatggatcc gaaaacttcc cctctgtgaa cgaaggaacg ttgtgcgctt
    22321 ttcgttcagc acaactaaga ctactcattt aaaatgcaaa atcaaaaaca atttccattt
    22381 taattaaaaa tacttgataa cttattgata acaatatgaa taaaatttat tcacttgctg
    22441 aggtgcgttt cggaaataaa aaaggcaccc gaaggtgcct gaggtaaggt gctgaatcgc
    22501 ttaacgatcg actatcacag aagattaatc cagcgttgga ttcatgtgcc gtagatcgta
    22561 tggcgtgatc tgatagacgt aatagttgag ccagttggta aacagcaaat taccgtgact
    22621 acgccagctc gctcgcggtt tgttttgcgg atcgttgtgc gggaaatagt tatacggtac
    22681 atccgggtct agtccggctt ccacatcgcg taaatattcc tgcgccagcg tttgcgcatc
    22741 atattcggga tggcccgtca caaaggcaat gcgcttatct ttgctggcaa acagatatgc
    22801 atccccttct tccgtctctg ccagaatttc cagatcggtg taatcacgaa tcaacgctgc
    22861 cggaaagtca gcatagcgcg aatgcggtgc caggaatgaa tcatcaaagc cacgcgtcag
    22921 aagcgcatga ggatggagaa tatgatgctc gtaaacgcca gagagtttgt cggtgcgagt
    22981 ttgcttagga atgccgtaga ggatattgag cgcggcctgt accgcccagc agacaaacag
    23041 cgtcgaggtg acgtgatctt tcgaccactc cagcacctgt ttgatctgcg gccagtaagc
    23101 gacatcatta aactccacca ggcccagcgg cgcaccggtt acaatcagac cgtcaaagtt
    23161 ctgttcctga atatcttcaa agttacagta gaagttgttc agatgctctg cgggcgtgtt
    23221 gcgcgattca cgggaatcga tgcgcaacag ctgaatatcg acctgcaaag gtgagtttga
    23281 aagcaggcgc agaaactgat tttcagtttc aatcttcttc ggcatcaggt taaggatcag
    23341 aaccttaagt ggacgaattt cctgaccaga cgcacgagaa gttgtcatca caaagacgtt
    23401 ttcttcacgc aagaaattga cggcgggtag ctcgtccggc acacgaatcg gcataacctg
    23461 attacctcac tacatacgct tatacgttta gacatccaga tagctgaaga taaccagaaa
    23521 atttgccaat gtcgagcctg catgttgaag gtgagaaagt ttcaaggaaa aggctacgtt
    23581 agaatataag aatgacgaaa aggagagaaa atggttatta gtattcgccg ctcacggcat
    23641 gaggaagggg aggaactcgt tgcgatttgg tgtcgatctg tcgatgccac ccacgatttt
    23701 ttatcagcag agtatcgggc cgagctggaa gagctggtgc gttcctttct gccggaagca
    23761 ccgttgtggg tcgcggttaa tgaacgggag cagccggttg ggtttatgtt gttaagtggg
    23821 cagcatatgg atgcgctgtt tatcgatcct gatgtgtgcg gctgcggcgt gggtcggatg
    23881 ctggtggagc atgcgctctc gatggcaccg gaactgacaa ccaacgttaa tgagcaaaat
    23941 gagcaggcgg ttgggttcta taagaaggtg ggttttaagg ttacgggacg ctctgaggtg
    24001 gacgatttgg ggaaaccgta tccgttgctg aatctggcgt atgtggggga ataagccgcc
    24061 tctgccgtgc ctgcccaagc tggagaagac gcgccagcgt cgcacccggc atttttttca
    24121 ccaacgcaaa aaggccatcc tgacggatag ccttt
//
LOCUS       DAJTRD010000059        23923 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTRD010000059 DAJTRD010000000
VERSION     DAJTRD010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.059"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(1..386)
                     /locus_tag="OO315_002551"
                     /pseudo
     CDS             complement(1..386)
                     /locus_tag="OO315_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            910..2838
                     /gene="thrS"
                     /locus_tag="OO315_002552"
     CDS             910..2838
                     /gene="thrS"
                     /locus_tag="OO315_002552"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5891415.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2842..3384
                     /gene="infC"
                     /locus_tag="OO315_002553"
     CDS             2842..3384
                     /gene="infC"
                     /locus_tag="OO315_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5891416.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3481..3678
                     /gene="rpmI"
                     /locus_tag="OO315_002554"
     CDS             3481..3678
                     /gene="rpmI"
                     /locus_tag="OO315_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5891417.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3731..4087
                     /gene="rplT"
                     /locus_tag="OO315_002555"
     CDS             3731..4087
                     /gene="rplT"
                     /locus_tag="OO315_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5891418.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    4204..4328
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4210..4254
                     /gene="pheM"
                     /locus_tag="OO315_002556"
     CDS             4210..4254
                     /gene="pheM"
                     /locus_tag="OO315_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5891419.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4537..5520
                     /gene="pheS"
                     /locus_tag="OO315_002557"
     CDS             4537..5520
                     /gene="pheS"
                     /locus_tag="OO315_002557"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5891420.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5535..7922
                     /gene="pheT"
                     /locus_tag="OO315_002558"
     CDS             5535..7922
                     /gene="pheT"
                     /locus_tag="OO315_002558"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5891421.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7927..8226
                     /gene="ihfA"
                     /locus_tag="OO315_002559"
     CDS             7927..8226
                     /gene="ihfA"
                     /locus_tag="OO315_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5891422.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8327..9307
                     /gene="btuC"
                     /locus_tag="OO315_002560"
     CDS             8327..9307
                     /gene="btuC"
                     /locus_tag="OO315_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5891423.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9370..9921
                     /gene="btuE"
                     /locus_tag="OO315_002561"
     CDS             9370..9921
                     /gene="btuE"
                     /locus_tag="OO315_002561"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5891424.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9921..10670
                     /gene="btuD"
                     /locus_tag="OO315_002562"
     CDS             9921..10670
                     /gene="btuD"
                     /locus_tag="OO315_002562"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5891425.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10748..11212
                     /locus_tag="OO315_002563"
     CDS             10748..11212
                     /locus_tag="OO315_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5891426.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11459..12172
                     /gene="ydiV"
                     /locus_tag="OO315_002564"
     CDS             11459..12172
                     /gene="ydiV"
                     /locus_tag="OO315_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5891427.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFVWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            12236..13672
                     /locus_tag="OO315_002565"
     CDS             12236..13672
                     /locus_tag="OO315_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5891428.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO315_002566"
     CDS             complement(13676..13867)
                     /gene="hemP"
                     /locus_tag="OO315_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5891429.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO315_002567"
     CDS             complement(13999..15045)
                     /gene="aroH"
                     /locus_tag="OO315_002567"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5891430.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO315_002568"
     CDS             complement(15202..16035)
                     /gene="ppsR"
                     /locus_tag="OO315_002568"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5891431.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            16367..18745
                     /gene="ppsA"
                     /locus_tag="OO315_002569"
     CDS             16367..18745
                     /gene="ppsA"
                     /locus_tag="OO315_002569"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5891432.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO315_002570"
     CDS             complement(18802..20448)
                     /gene="fadK"
                     /locus_tag="OO315_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5891433.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20505..20798)
                     /locus_tag="OO315_002571"
     CDS             complement(20505..20798)
                     /locus_tag="OO315_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5891434.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20795..22084)
                     /locus_tag="OO315_002572"
     CDS             complement(20795..22084)
                     /locus_tag="OO315_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5891435.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(22140..23078)
                     /locus_tag="OO315_002573"
     CDS             complement(22140..23078)
                     /locus_tag="OO315_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5891436.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(23098..23862)
                     /locus_tag="OO315_002574"
     CDS             complement(23098..23862)
                     /locus_tag="OO315_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5891437.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
BASE COUNT         5909 a         5802 c         6298 g         5914 t
ORIGIN      
        1 attaccctgt tcctgttcaa tataatccag tatgcttcgc aaatggttag ctttaatagg
       61 cgtggtttct agtaataagg aaatgatttc ttttttatag tgtaatgtaa tgagtatatt
      121 ttctgcatgg atgggtgaag tggcaaagtt aatggtaaaa agggcagtgg aataacaatt
      181 ttgtgatatt ttcgacagtt tgcggtaaat tcctgagtaa cacagtatga cagcatcgaa
      241 cttaagtcag aggtatcaag tagggtgatg agctggcgcg caaatttatt aataggctct
      301 gatgaaggtg ttggtatgtc gctatcgatg tgcaatctat gaggagactt ataatgagtt
      361 ctgataatga tatcgttttg actcataatc tattcgcctt atatatatct ggaggttttg
      421 aggatgatga ttaattatta tcgccagaac gaaatgtgca cgaaaaattg ttataagaat
      481 tgtgtcactt ttatcattgt gatatgacca cttcgtcatt aatctcatga gcgtttaaaa
      541 tactaccgcc aaatcacgca acgaactttg ctggattcag tttatgctgc tgtaaatccg
      601 ctcgcagtaa acctttcaga cgcaccgtga tgttatcagt ggttctttaa gcgttttgct
      661 ggtgtactca ctacaaacga attgcgaatc gaatcaatgt gaaacggaaa ggtacaatct
      721 cccttttttt agtgttaaca tcgttcaacc gggtggaagg tgagaggtta atgtctttca
      781 tttgtgtacc tgattgcgaa ccaatttagc atttgttggc tagatggttt cgcaatgaac
      841 tgttaataaa caaatttttc tttgtatgtg atctttcgtg tgggtcacca ctgcaaataa
      901 ggatataaaa tgcctgttat aactcttcct gatggcagcc aacgccatta cgaccacgct
      961 gtaagcccca tggatgttgc gctggacatt ggtccaggtc tggcgaaagc ctgtatcgca
     1021 gggcgcgtta atggcgaact ggttgatgct tgcgatctga ttgaaaacga cgcacaactg
     1081 tcgatcatta ccgccaaaga cgaagaaggt ctggagatca ttcgtcactc ctgtgcgcac
     1141 ctgttagggc atgcgattaa acaactttgg ccgcatacca aaatggcaat cggcccggtt
     1201 attgacaacg gtttttatta cgacgttgat cttgaccgca cgttaaccca ggaagatgtc
     1261 gaagcactcg agaagcggat gcatgagctt gctgagaaaa actacgacgt cattaagaag
     1321 aaagtcagct ggcacgaagc gcgtgaaact ttcgccaacc gtggggagag ctacaaagtt
     1381 tccattcttg acgaaaacat cgcccatgat gacaagccag gtctgtactt ccatgaagaa
     1441 tatgtcgata tgtgccgcgg tccgcacgta ccgaacatgc gtttctgcca tcatttcaaa
     1501 ctaatgaaaa cggcaggggc ttactggcgt ggcgacagca acaacaaaat gttgcaacgt
     1561 atttacggta cggcgtgggc agacaaaaaa gcacttaacg cttacctgca gcgcctggaa
     1621 gaagccgcga aacgcgacca ccgtaaaatc ggtaaacagc tcgacctgta ccatatgcag
     1681 gaagaagcgc cgggtatggt attctggcac aacgacggct ggaccatctt ccgtgaactg
     1741 gaagtgtttg ttcgttctaa actgaaagag taccagtatc aggaagttaa aggtccgttc
     1801 atgatggacc gtgtcctgtg ggaaaaaacc ggtcactggg acaactacaa agatgcaatg
     1861 ttcaccacat cttctgagaa ccgtgaatac tgcattaagc cgatgaactg cccgggtcac
     1921 gtacaaattt tcaaccaggg gctgaagtct tatcgcgatc tgccgctgcg tatggccgag
     1981 tttggtagct gccaccgtaa cgagccgtca ggttcgctgc atggcctgat gcgcgtgcgt
     2041 ggatttaccc aggatgacgc gcatatcttc tgtactgaag aacaaattcg cgatgaagtt
     2101 aacggatgta tccgtttagt ctatgatatg tacagcactt ttggcttcga gaagatcgtc
     2161 gtcaaactct ccactcgtcc tgaaaaacgt attggcagcg acgaaatgtg ggatcgtgct
     2221 gaggcggacc tggcggttgc gctggaagaa aacaacatcc cgtttgaata tcaactgggt
     2281 gaaggcgctt tctacggtcc gaaaattgaa tttaccctgt atgactgcct cgatcgtgca
     2341 tggcagtgcg gtacagtaca gctggacttc tccttgccgt ctcgtctgag cgcctcctat
     2401 gtgggcgaag acaacgagcg taaggtaccg gtaatgattc accgcgcaat tctggggtcg
     2461 atggagcgtt tcatcggtat cctgaccgaa gaattcgctg gtttcttccc gacctggctt
     2521 gcgccagttc aggttgttat catgaatatt accgattcac agtctgaata cgttaacgaa
     2581 ttgacgcaaa aactatcaaa tgcgggcatt cgtgttaaag cagacttgag aaatgagaag
     2641 attggcttta aaatccgcga gcacactttg cgtcgcgtcc catatatgct ggtctgtggt
     2701 gataaagagg tggaatcagg caaagttgcc gttcgcaccc gccgtggtaa agacctggga
     2761 agcatggacg taaatgaagt gatcgagaag ctgcaacaag agattcgcag ccgcagtctt
     2821 aaacaattgg aggaataagg tattaaaggc ggaaaacgag ttcaaacggc gcgccctaac
     2881 cgtatcaatg gcgaaattcg cgcccaggaa gttcgcttaa caggtctgga aggcgagcag
     2941 cttggtattg tgagtctgag agaagctctg gagaaagcag aagaagccgg agtagactta
     3001 gtcgagatca gccctaacgc cgagccgccg gtttgtcgta taatggatta cggcaaattc
     3061 ctctatgaaa agagcaagtc ttctaaggaa cagaagaaaa agcaaaaagt tatccaggtt
     3121 aaggaaatta aattccgtcc tagtacagat gaaggcgact atcaggtaaa actccgcagc
     3181 ctgattcgct ttctcgaaga gggtgataaa gccaaaatca cgctgcgttt ccgcggtcgt
     3241 gagatggcgc accagcaaat cggtatggaa gtgcttaatc gcgtgaaaga cgatttgcaa
     3301 gaactggcag tagtcgaatc cttcccaacg aagatcgaag gccgccagat gatcatggtg
     3361 ctcgctccta agaagaaaca gtaaggcctt caagtagcac tatctgtgga gcctttgggc
     3421 ttcacaggca ttgttcgcct ttgtttcgtt tattaacaat gcgaagtgga agttattaaa
     3481 atgccaaaaa ttaagaccgt acgcggtgct gctaagcgct tcaaaaaaac cggtaaaggt
     3541 ggttttaagc acaagcacgc taacctgcgt cacattctga ctaaaaaagc gaccaaacgt
     3601 aaacgtcacc tgcgtccgaa agccatggtt tccaaaggcg atctgggcct ggtaatcgcg
     3661 tgcctgccgt acgcataagc cgttaacgtt tttaactttt taattagaat atagatacag
     3721 gagagcacat atggctcgcg taaaacgtgg tgttattgca cgcgcacgtc acaagaaaat
     3781 tttgaaacaa gctaaaggct actacggtgc gcgttctcgc gtataccgcg ttgccttcca
     3841 ggctgttatc aaagctggtc agtatgctta ccgtgaccgt cgtcaacgta agcgtcagtt
     3901 ccgtcaactg tggattgcgc gtatcaacgc agcagcacgt cagaacggta tttcttacag
     3961 caaattcatc aatggcctga aaaaagcctc tgttgaaatc gaccgtaaga tcctggctga
     4021 tatcgcagta ttcgacaaag tagcgttcac cactctggtt gaaaaagcga aagcagctct
     4081 ggcataagcc agttgaaaga gggagctagt ctccctcttt tcgtttcaac gccatcaaaa
     4141 cattgacttt tatcgccgta gccttttcaa taaaggtctt ttgaagagta ccaaaaggta
     4201 acgcaagcaa tgaatgctgc tattttccgc ttcttttttt actttagcac ctgaatccag
     4261 gaggctagcg cgtgagaaga gaaacggaaa acagcgcctg aaagcctccc agtggaggct
     4321 ttttttgtat gcgcgttttg aaaaataaaa agccagtcta tcaggcgtaa ttgtcgcagc
     4381 cagtctggac acggccagtg cggagaaacc ggagcgtact taagtacgtg aggatttcga
     4441 gcactgcccg ggaccaaaat ggcaagtaaa atagcctgat gggataggct ctaagtccaa
     4501 cgaaccagtg tcaccactga cacaatgagg aaaaccatgt cacatctcgc agaactggtt
     4561 gccagtgcga aggcggccat tagccaggcg tcagatgttg ccgcgttaga taatgtgcgc
     4621 gtcgaatatt tgggtaaaaa agggcactta acccttcaga tgacgaccct gcgtgagctg
     4681 ccgccagaag agcgtccggc agctggtgcg gttatcaacg aagcgaaaga gcaggttcag
     4741 caggcgctga atgcgcgtaa agcggaactg gaaagcgctg cactgaatgc gcgtctggcg
     4801 gcggaaacga ttgatgtctc tctgccaggt cgtcgcattg aaaacggcgg tctgcatccg
     4861 gttacccgta ccatcgaccg tatcgaaagt ttcttcggtg agcttggctt taccgtggca
     4921 accgggccgg aaatcgaaga cgattatcat aacttcgatg ctctgaacat tcctggtcac
     4981 cacccggcgc gcgctgacca cgacactttc tggtttgacg ctacccgcct gctgcgtacc
     5041 cagacctctg gcgtacagat ccgcaccatg aaagcccagc agccaccgat tcgtatcatc
     5101 gcgcctggcc gtgtttatcg taacgactac gaccagactc acacgccgat gttccatcag
     5161 atggaaggtc tgattgttga taccaacatc agctttacca acctgaaagg cacgctgcac
     5221 gacttcctgc gtaacttctt tgaggaagat ttgcagattc gcttccgtcc ttcctacttc
     5281 ccgtttaccg agccttctgc agaagttgac gtcatgggta aaaacggtaa atggctggaa
     5341 gtgctgggct gcgggatggt gcatccgaac gtgctgcgta acgttagcat cgacccggaa
     5401 gtttactctg gtttcgcctt cgggatgggg atggagcgtc tgactatgtt gcgttacggc
     5461 gtcaccgacc tgcgttcatt cttcgaaaac gatctgcgtt tcctcaaaca gtttaaataa
     5521 ggcaggaata gattatgaaa ttcagtgaac tgtggttacg cgaatgggtg aacccggcga
     5581 tcgatagcga tacgctggcg aatcaaatca ctatggcggg cctggaagtt gacggtgtag
     5641 aaccggttgc cggtagcttc cacggcgtgg tcgttggtga agtggttgag tgtgcgcagc
     5701 atccgaacgc tgacaaactg cgtgtgacaa aagtgaatgt cggcggcgat cgcctgctgg
     5761 acatcgtctg cggtgcgcca aactgccgtc agggcctgcg tgtggcggta gctaccattg
     5821 gtgctgttct gccgggtgat ttcaaaatta aagcggcgaa actgcgtggc gaaccgtctg
     5881 aagggatgct gtgctccttt tctgagctgg gaatttctga cgatcacagt ggcattatcg
     5941 aactgcctgc ggatgcgccg attggcaccg acatccgcga atacctgaaa ctcgatgata
     6001 acaccatcga aatcagcgtg acaccaaacc gtgccgactg cttaggtatc attggtgttg
     6061 cgcgtgacgt tgccgtgctg aatcagctgc cgctggttga accggaaatc gttccggttg
     6121 gtgcgactat cgacgacacg ttgccgatta cagtcgaagc gccggaagcc tgcccgcgct
     6181 atcttggccg tgtggtaaaa ggcattaacg ttaaagcgcc aactccgctg tggatgaaag
     6241 aaaaactgcg tcgttgcggg atccgttcta tcgatgcagt tgttgacgtc accaactatg
     6301 tactgctcga actgggccag ccgatgcacg ctttcgataa agatcgcatt gaaggcggca
     6361 ttgtggtgcg gatggcaaaa gagggcgaaa cgctggtgct gcttgatggc actgaagcga
     6421 agctgaatgc tgacactctg gtcatcgccg accacaacaa ggcgctggcg atgggcggca
     6481 tctttggtgg cgaacactct ggcgtgaatg acgaaacgca aaacgtcttg ctggaatgcg
     6541 ctttcttcag cccgctgtct atcaccggtc gtgctcgtcg tcatggcctg catactgatg
     6601 cgtctcaccg ttatgagcgt ggcgttgatc cggtactgca gcacaaagcg atggaacgtg
     6661 cgacccgtct gctgatcgac atctgcggtg gtgaggctgg cccggtaatt gatatcacca
     6721 acgaagcaac gctgccgaag cgtgcaacca tcactctacg tcgtagcaaa ctggatcgcc
     6781 tgataggcca tcatattgct gatgagcagg taactgacat tctgcgtcgt ctcggctgcg
     6841 aagtgaccga aggcaaagac gaatggcagg cagttgcgcc gagctggcgt ttcgatatgg
     6901 agattgaaga agatctggtc gaagaagtcg cgcgtgttta cggctacaac aacatcccgg
     6961 atgagccggt acaggcaagc ctgattatgg gtactcaccg tgaagctgac ctgtcgctca
     7021 agcgcgtgaa aacgctgctc aacgacaaag gctatcagga agtgatcacc tacagcttcg
     7081 ttgatccgaa agtgcagcag atgatccatc caggcgttga agccttactg ctggcaagcc
     7141 cgatctctgt tgaaatgtca gcaatgcgtc tttctctgtg gaccggcctg ctggcaaccg
     7201 tggtgtacaa ccagaaccgt cagcagaacc gtgtgcgcat tttcgaaagc ggtctgcgtt
     7261 tcgtaccaga tactcaggca ccgttgggca ttcgtcagga tctgatgtta gccggtgtga
     7321 tttgcggtaa ccgttacgaa gagcactgga acctggcaaa agagaccgtt gatttctatg
     7381 atttgaaagg cgaccttgaa tccgttctcg acctgaccgg taaactgaat gaggttgagt
     7441 tccgtgcaga agcgaatccg gcactgcatc cggggcaatc cgcagcgatt tatctgaaag
     7501 gtgaacgtat tggttttgtt ggagttgttc atcctgaact ggaacgtaaa ctggatctta
     7561 acggtcgcac tctggtgttc gaactggagt ggaacaagct cgcagaccgc gtggtgcctc
     7621 aggcgcgcga gatttctcgc ttcccggcga accgtcgtga catcgcggtg gtggtcgcag
     7681 aaaacgttcc cgcagcggat attttatccg aatgtaagaa agttggcgta aatcaggtag
     7741 ttggcgtaaa cttatttgac gtgtaccgcg gtaagggtgt tgcggagggg tataagagcc
     7801 tcgccataag cctgatcctg caagatacca gccgtacact cgaagaagag gagattgccg
     7861 ctaccgtcgc caaatgtgta gaggcattaa aagagcgatt ccaggcatca ttgagggatt
     7921 gaacctatgg cgcttacaaa agctgaaatg tcagaatatc tgtttgataa gcttgggctt
     7981 agcaagcggg atgccaaaga actggttgaa ctgtttttcg aagagatccg tcgcgctctg
     8041 gaaaacggcg aacaggtgaa actctctggt tttggtaact tcgatctgcg tgataagaat
     8101 caacgcccgg gacgtaaccc gaaaacgggc gaggatattc ccattacagc acggcgcgtg
     8161 gtgaccttca gacccgggca gaagttaaaa agccgggtcg aaaacgcttc gcccaaagac
     8221 gagtaatctg atctaactaa aaaggccgct atgcggcctt ttttcttttc actgtcgaag
     8281 agtcaccgta aaatcaacgc catgacactt cagcagaacg gataccatgc tgacacttgc
     8341 ccgccaacaa cagcgacaaa atattcgctg gttattatgc ctgtcagttt tgatgctgct
     8401 ggcgcttctc ttaagccttt gcgccggtga acaatggatt tcgccaggtg actggtttac
     8461 tcctcgtggc gaactgttcg tctggcaaat tcgcctgcca cgtacgctgg ctgtattgct
     8521 ggttggtgcg gcgctggcta tatccggcgc tgtaatgcag gcgttgtttg aaaatcctct
     8581 ggcagaacct ggactacttg gcgtctctaa cggcgcaggc gtggggctta tcgccgcggt
     8641 attgcttggg caagggcaac tccccaactg ggcgctaggg ctgtgtgcga ttgctggcgc
     8701 gcttatcatc actttaatac tcttacgttt cgcccgtcgt catctttcga ccagtcggtt
     8761 attgctggct ggcgttgcat tagggattat ctgcagcgca ctaatgacgt gggctatcta
     8821 cttttccacc tctgttgatt tacgtcagct gatgtactgg atgatgggcg gttttggcgg
     8881 cgtagactgg cggcaaagct ggctgatgct ggcattgatc cccgtgttgt tgtggatctg
     8941 ttgtcagtcc aggccgatga atatgttagc gcttggcgag atctcggcgc ggcaactggg
     9001 tttacccatg tggttctggc gcaatgtgct ggtggcagcg accggctgga tggttggcgt
     9061 cagtgtggcg ctggcgggtg ctatcggctt tattggtctg gtgatcccac atattcttcg
     9121 gttgtgtggt ttaaccgatc atcgcgtatt acttcccggc tgcgcgctgg caggggcgag
     9181 cgcattgctg ctggccgata ttgtagcgcg cctggcatta gctgccgcag agctgcctat
     9241 tggcgtggtc accgcaacgt tgggtgcgcc ggtgtttatc tggttattgt taaaagcagg
     9301 acgttagccg caaaaagacg gtctatgatt aaaagctaag atttttactg accacaccta
     9361 ggagaaacga tgcaagattc cattctgacg accgtagtga aagatatcga cggtgaagtg
     9421 accacgctgg agaagtacgc cggtaatgtg ctgttaattg tcaatgtcgc ctcaaagtgt
     9481 ggcttaacgc cgcaatatga gcagttggag aatattcaaa aagcctgggc cgatcgaggt
     9541 tttgtggtgc tgggattccc gtgcaaccag tttctggaac aagaaccggg cagcgatgaa
     9601 gagattaaaa cttactgtac caccacatgg ggggtgacgt tcccgatgtt cagtaagatt
     9661 gaagttaatg gcgaaggacg ccatccgctg tatcaaaaat tgattgccgc agcgccgacc
     9721 acagtcgcgc cggaagagag cggattctat gcccgtatgg tcagcaaagg ccgtgcaccg
     9781 ctgtacccgg atgatatttt atggaatttt gaaaaattcc tggttggcag ggacggaaaa
     9841 gtcatccagc gtttttcccc ggatatgacg ccggaagatc ccattgtgat ggaaagcatt
     9901 aaactggcgt tggcaaaata atgtctattg tgatgcagtt acaggatgtt gcggaatcta
     9961 cccgcctggg gccgctttca ggcgaggttc gggctgggga aatcctgcac ctggtggggc
    10021 cgaatggcgc gggtaagagt accttactgg cgcgaatggc cggaatgacc agcggtaagg
    10081 gaagcattca gttcgcgggg caaccactgg aagcatggtc cgcaacaaaa ctcgcgctgc
    10141 atcgcgccta tctttcacaa cagcagacgc cgccgtttgc aatgccggtc tggcactacc
    10201 tgacactgca tcagcacgat aaaacgcgta ccgaactact gaatgatgtc gcaggggcgc
    10261 tggctcttga tgacaaactc ggtcgtagca ccaatcaact ttccggcggt gaatggcaac
    10321 gcgtacgtct tgctgcggtg gtgttgcaaa tcacaccaca agccaatccc gcaggccaat
    10381 tgctgcttct tgatgagccg atgaacagtc ttgatgttgc gcaacaaagt gcgttagaca
    10441 aaattctgag cgcgctgtgt cagcaaggac tggcgattgt gatgagcagt cacgatctca
    10501 accacacgtt gcgtcatgcg catcgggcgt ggttgctaaa aggtggaaaa atgctggcca
    10561 gtggacgcag agaagaggtg ctcacgccgc caaatctggc gcaggcctat gggatgaatt
    10621 ttcgccgtct ggatatcgaa ggtcacagaa tgctgatttc gaccatctga tttcattagt
    10681 atcccgaaaa tcgggtaatt acgacgctaa attaatgcaa gaataaaaaa cagaggaacc
    10741 gtacggaatg cgtttctgcc ttattttgat cacagcactg tttctggccg ggtgtagcca
    10801 ccataaagca ccgccgccaa atgccagact ttctgattcg attaccgtta ttgccggttt
    10861 gaacgaccag ctacaaagct ggcatggcac gccgtatcgt tatggtggca tgacgcggcg
    10921 cggtgtggac tgttcgggat ttgtggttgt gacgatgcgc gatcgtttcg atttgcatct
    10981 gccccgtgaa accaaacaac aagcctctat tggcacgcaa attgataaag acgagttgct
    11041 gcctggtgac ctggtctttt tcaaaacggg ttccggacaa aatggtttgc atgtaggtat
    11101 ttacgatacc aacaaccaat ttatccacgc ctctaccagc aagggagtga tgcgttcctc
    11161 acttgataat gtctattggc agaaaaattt ctggcaggcg agacgaatct agtatgtcga
    11221 aaaattgacg gcgatgatac atcgccgtca atttactcct gaatacagta gtattttgca
    11281 gacaaaaatt ccgtaaatgg attattcgtt tataatcagg ataatctaaa aaaaggcccc
    11341 ctgaaaaagt ggttaacatg gaacaaatga aagttactcc ttctgtaatt gttatgtctt
    11401 atggcctcat cccttttagc cggatactga aaaacatcct tcgagaggga cggttaccat
    11461 gaagattttt ttggagaatc tttatcattc tgattgctat ttcctgccga taagagataa
    11521 ccagcaactc cttgtcggcg ttgagttaat tacccatttc tccagtgaag atggcacggt
    11581 tcgtatcccg acgagtcggg ttatagcgca acttaccgag gagcaacatt ggcaattatt
    11641 ttcagagcag ttggaattac taaaatcatg ccaacacttt tttattcaac ataaactctt
    11701 tgtctggctt aatttaacgc cacaagtggc tacattatta ttagacaggg ataattacgc
    11761 aggcgaacta ttaaaatatc cgtttattga gctgttgatt aatgagaatt acccgcatct
    11821 caatgaagga aaagataatc gcgatctttt gtccttatcg caaatgtatc cccttgtgtt
    11881 aggcaatttg ggagcaggta atagcacaat gaaagcggtt tttgatggtc ttttcacccg
    11941 cgtaatgctg gagaaaggtt ttattcagca acaaatcacg catcgctcct tcgaaccgtt
    12001 tattcgtgcg atccaggcgc aaatttcccc ttgttgcaat tgcatcatcg cggggggcat
    12061 tgatacgcca gaaatattgg cacagattac ccccttcgat ttccatgctc tccagggatg
    12121 cctgtggcct gccgttccta taaatcagat aacgacgttg gttcagcgat aaccctcctg
    12181 tttgctggtg tttaagacga gagtaaccgt ctacactatc aaacagatag gatctatgac
    12241 cctgtctttt attacccgct ggcgcgatga attgccagaa acctatacag cactttcccc
    12301 tacgccttta aataatgccc ggctgatttg gcataatacc gaactcgcta acacgctgag
    12361 tattccatcg tcgctgttta aaaatgccgc aggtgtctgg ggcggcgaaa ccctactgcc
    12421 tggtatgtca ccactggcac aggtttacag tggtcatcag ttcgtcgtct gggcgggcca
    12481 actgggtgat gggcgcggca ttttacttgg cgaacaactg cttgctgatg gcactacaat
    12541 ggactggcat ctgaaaggtg ctggcctgac gccttattcg cgaatgggtg atggacgggc
    12601 ggttttacgt tcgacgatac gagaaagtct cgccagtgag gcgatgcatt atctgggcat
    12661 tccgacgacc cgcgcgttaa gtatcgtcac cagcgattcg ccagtgtatc gggaaacggt
    12721 ggagccaggc gcgatgctga tgcgtgtggc accaagtcat ctgcgctttg gtcatttcga
    12781 acatttttac tatcgccgcg agccggaaaa ggttcgtcag ttggctgact ttgccattcg
    12841 tcattactgg tcacatcttg aagacgatga ggacaaatac cgtctctggt ttaacgatgt
    12901 tgtcgcacgt accgcgtcgt taattgccca atggcagacg gtcggctttg cacatggggt
    12961 gatgaatacc gacaacatgt cgctgctggg actgacgctt gattacgggc cgtttggttt
    13021 tcttgatgat tacgaacccg gttttatttg taatcactcg gatcatcaag ggcgttatag
    13081 ctttgataat caacctgctg tcgcgttgtg gaatttacag cgtctggcgc agacattgtc
    13141 accatttgtt gccgtagatg ccctgaatga ggccctggac agctatcagc aggttttgtt
    13201 gacgcattat ggacaacgga tgcggcagaa actgggcttc atgacggagc aaaaagagga
    13261 taacgcgcta ctgaatgaat tattcagtct gatggcgcga gagcgcagcg attatacccg
    13321 cacattccgc atgctgagtc tgaccgagca gcacagcgcg gcgtcaccgc tacgtgagga
    13381 gtttattgat cgtgcggcat ttgatgactg gtttgcccgt tatcgggggc gtttgcaaca
    13441 agacgaggtt agcgatagtg agcgtcagca actgatgcaa agcgttaacc cagctctggt
    13501 attgcgcaac tggttggcgc aacgggcgat tgaagcggca gaaaagggtg atatgatgga
    13561 attgcaccgc ctgcatgagg cgttgcgaaa tcctttcagc gacagagatg atgactatgt
    13621 cagtcgtcca cctgactggg gtaaacggct ggaagtcagc tgctcgagtt aaaggctact
    13681 tagttaacag cagcttgcca gcctgagttt tacggagcag atattcttgc ccgtcatgat
    13741 cgataatcag tttgccatcc ggacctaaca gtgtctggct ggtaattcgc cggatgggct
    13801 gcggggaagc ggtcttgtga ttggcatccg tttcaggcgt gagttttctg ctatccgtat
    13861 aacgcataaa aattaataat aattgatata acaatgattc ctattatcaa tattatggca
    13921 gaccagagca agcagtaatg tgttgagaag acaacaagtt gcgcagaaat gcccatccca
    13981 tcaggaatgg gcacgcattc agaagcgggt atctaccgca aaggcgagtt tttcgaccag
    14041 gcgttcggta tcttcccagc ccagacacgg gtcggtaatg gattgaccgt aagtgagcgg
    14101 ctgaccgccg acgatttttt gcgttccttc gcgcaggaaa ctttccgcca taattccagc
    14161 aatctccgta gagccattgc ggatttgctg acaaatatcc tcacaaactt ctaactggcg
    14221 acggtgctgc ttctggcagt taccgtggct gaaatccacc accagatgtt caggtaaatc
    14281 aaactcgtgc agcgtatcgc aggctgcggc gatatcatcg gcatgataat tcggtttttt
    14341 gccgccacgc ataataatgt ggccatacgg gttgccgctg gtctgataga tggtcatctg
    14401 accatttttg tctggcgaga ggaacatatg gctggcgcgg gctgcgcgga tagcatccac
    14461 ggcaatccgc gtgttgccat cggtaccatt tttaaaacct accggacagg agagtgctga
    14521 agccatttcg cggtggatct gactttcggt agtacgagcg ccaatcgcgc cccaactgat
    14581 taaatcagca ataaactgac cggttaccat atcgaggaac tcagtcgcgg tagggacacc
    14641 cagctcattt acctgtaaaa gtaatttgcg cgccagctcc agaccgtgat ttacccgata
    14701 gctgccgttt aaatccggat cggagattag ccctttccag ccgacaacag ttcgtggttt
    14761 ttcaaaatag gtgcgcatta cgatttccag ccgtgactgg tactggttgc gcagcgactg
    14821 cagacgggtg gcgtactcca ttgcagcggt gagatcgtgg atcgagcagg ggccaataat
    14881 gaccaacagt cgcttatctt caccattcag tattttttca attctgcggc gggagtcggt
    14941 gacatgggtg gcgacgccag gcgttacggg ataccgtagc gcgagttcgg cgggcgttac
    15001 caggctctca atacgcgcag tacggagttc gtcagttctg ttcattacaa gtctcagaaa
    15061 tttgttgctt ctacggcagg actaccggaa agtgatgcgc atcacgataa accaatcgca
    15121 gctgatttca agtgcgaggt gtgtcacacc tcgccgggtg gttagcatga taacaaaaaa
    15181 acaaaccaat gcactagttc tctagtacat tcggcgacta aggcccatga tatcgaggat
    15241 cttggtggca atctcttcta ccgaataatt ggtactgtta atccacggga tctgattttt
    15301 acggtacaag gcttccactt ccgcgacttc catcctgcac tgacgaagcg aggcatagcg
    15361 actgttctcc cgacgttcct cgcgaatcgc cgccagacgt tccgggtcga tagtcaggcc
    15421 gaacaattta tgctgaagcg gtttgagcga cgcgggtagc accagattat ccatatcgtc
    15481 ggcaataaag gggtagtttg ccgcgcggat accaaattgc attgccagat acagactggt
    15541 gggggtttta ccacagcgag aaacaccgag caggatcacc tgagcctgat ccagattgcg
    15601 caacgaaatg ccgtcatcgt gggcgagggt gtaatcaatc gccgcaatgc gcgcatcata
    15661 tttattgaga ttattagggt taaggccatg ggtacgatga gcaatcggcg ttggatccag
    15721 tttcatctct tgttgtagcg gggcaaccag cgcctgaacg atatcctggc aaaagccttc
    15781 actttgcaag atgatggcgc gaatctccgg caacacgatg gagtagaaga ccagcgggcg
    15841 cacgcctgtc tggtgataaa ttgcgtcaat ctgatccttc actgcccgtg cacggctctc
    15901 attttcgaca aacggcagcg tgatgctgct gatagtgacg ggaaattgtg acattactgc
    15961 gtgtcctaat acctccgcag ttattgccgt accatcagaa atataaaaaa cgtggcgatc
    16021 aacagcatta tccattttgt tcttcccgtg aagcagacat aattctatga aagcataaat
    16081 taaaagcgca cagaagcgta ggacgtaatg tctggtttat aaaatgaacc ttcattttat
    16141 tttttataaa aacagcattt catttttatg gtttcgttta taccgatggt ttatgtggaa
    16201 attgtcgaat agagcaggtt tgcgcaacgc tgggatcagt cttaaaaagt aaaaaaatat
    16261 atttgcttga acgattcacc gtttttttca tccggttaaa tatgcaaaga taaatgcgca
    16321 gaaatgtgtt tctcaaaccg ttcatttatc acaaaaggat tgttcgatgt ccaacaatgg
    16381 ctcgtcaccg ctggtgcttt ggtataacca actcggcatg aatgatgtag acagggttgg
    16441 gggcaaaaat gcctccctgg gtgaaatgat tactaatctt tccggaatgg gtgtttccgt
    16501 tccgaatggt ttcgccacaa ccgccgacgc gtttaaccag tttctggacc aaagcggcgt
    16561 aaaccagcgc atttatgaac tgctggataa aacggatatt gacgatgtta ctcagcttgc
    16621 gaaagcgggc gcgcaaatcc gccagtggat tatcgacact cccttccagc ctgagctgga
    16681 aaacgccata cgcgaagcct atgcacagct ttccgccgat gacgaaaacg cctcttttgc
    16741 ggtacgctcc tccgccaccg cagaagacat gccggacgct tcctttgccg gtcagcagga
    16801 aaccttcctc aacgttcagg gttttgacgc cgttctcgtg gcagtgaaac atgtatttgc
    16861 ttctctgttt aacgatcgcg ccatctctta tcgtgtgcac cagggttacg atcaccgtgg
    16921 cgtggcgctc tccgccggtg ttcagcggat ggtgcgctcc gacctcgcat cttctggcgt
    16981 gatgttctcc attgataccg aatccggctt tgaccaggtg gtgtttatca cttccgcatg
    17041 gggccttggg gaaatggtcg tgcagggtgc ggttaacccg gatgagtttt acgtgcataa
    17101 accgacactg gcggcgaatc gcccggctat cgtgcgccgc accatggggt cgaaaaaaat
    17161 ccgcatggtt tacgcgccga cccaggagca cggcaagcag gttaaaatcg aagacgtacc
    17221 gcaggaacag cgtgacatct tctcgctgac caacgaagaa gtgcaggaac tggcaaaaca
    17281 ggccgtacaa attgagaaac actacggtcg cccaatggat attgagtggg cgaaagatgg
    17341 ccacaccggc aaactgttca ttgtgcaggc gcgtccggaa accgtgcgct cacgcggtca
    17401 ggtcatggag cgttatacgc tgcattcaca gggtaagatt atcgccgaag gccgtgctat
    17461 cggtcatcgc atcggcgcgg gtccggtgaa agtcatccat gacatcagcg aaatgaaccg
    17521 catcgaacct ggcgacgtgc tggttactga catgaccgac ccggactggg aaccgatcat
    17581 gaagaaagca tctgccatcg tcaccaaccg tggcggtcgt acctgtcacg cggcgatcat
    17641 cgctcgtgaa ctgggcattc cggcggtagt gggctgtgga gatgcaacag aacggatgaa
    17701 agacggtgag aacgtcactg tttcttgtgc cgaaggtgat accggttacg tctatgcgga
    17761 gttgctggaa tttagcgtga aaagctccag cgtagaaacg atgccggatc tgccgttgaa
    17821 agtgatgatg aacgtcggta acccggaccg tgctttcgac ttcgcctgcc tgccgagcga
    17881 aggcgtgggc cttgcgcgtc tggaatttat catcaaccgt atgattggcg tccacccacg
    17941 cgcactgctt gagtttgacg atcaggaacc gcagttgcaa aacgaaatcc gcgagatgat
    18001 gaaaggtttt gattctccgc gtgaatttta cgttggtcgt ctgactgaag ggatcgcgac
    18061 gctgggtgcc gcgttttatc cgaagcgcgt cattgtccgt ctctctgatt ttaaatcgaa
    18121 cgaatatgcc aacctggtcg gtggtgagcg ttacgagcca gatgaagaga acccgatgct
    18181 cggcttccgt ggtgcgggcc gctatgtttc cgacagcttc cgcgactgct tcgcactgga
    18241 gtgtgaagca gtgaaacgtg tgcgcaacga catggggctg accaacgttg agatcatgat
    18301 cccgttcgtg cgtaccgtag atcaggcgaa agcggtggtt gaagaactgg cgcgtcaggg
    18361 gctgaaacgt ggcgagaacg ggctgaaaat catcatgatg tgtgaaatcc cgtccaacgc
    18421 cttgctggcc gagcagttcc tcgaatattt cgacggcttc tcaattggct caaacgatat
    18481 gacgcagctg gcgctcggtc tggaccgtga ctccggcgtg gtgtctgaat tgttcgatga
    18541 gcgcaacgat gcggtgaaag cactgctgtc gatggctatc cgtgccgcga agaaacaggg
    18601 caaatatgtc gggatttgcg gtcagggtcc gtccgaccac gaagactttg ccgcatggtt
    18661 gatggaagag gggatcgata gcctgtctct gaacccggac accgtggtgc aaacctggtt
    18721 aagcctggct gaactgaaga aataaaataa atccccggcg gcgttcagtc gccggggtta
    18781 tgtgatcccc gaagatgaaa cttattcaat ctcttcacag acatcctgcg ttaatcgccg
    18841 tataatatct tttcttaaca aaaacttttg tattttatct gaggcagtgc gcggtagttt
    18901 ttcgattacc acgatatgtt caggatattt atattttgcg acccgtttac ggctaaaaaa
    18961 agccgccatc tcttccagcg ataatgaatg atgcggcgct ttcagcacga cataagcgca
    19021 tgatcgttca cctaaacgtt catcgggcat tgcaaccaca caggcatcgt gaattttagg
    19081 atgctgcaat aaaatatctt ccacttcacg gctgctaata ttttcgccgc cgcggacaat
    19141 aatatctttt ttgcgtccgg taatttttat atagccagcc tcatccatac ggcagagatc
    19201 gccgctgtaa taccagcctt cttcatccag cgcacgggcg gttaattcag gttcatcaaa
    19261 ataccccata aacacattgg ggccacgcga tgcttcttca ccttcgcaac ctggcggtaa
    19321 ggtcttgcgt gcgtcatcga ccactttaat ctctacacct gcggcagcgt aaccatcggt
    19381 atgcataaag cgcgacaaag aattatcgag attcaccacc gcatgcggcg aactttctgt
    19441 ggaaccataa acacttaata atttaatgct gcgctgctgg cattcgcgcg ccactttttt
    19501 ggggattgtg gtaccgccgc aaagaaagaa gcgcagcgct gaaaggtctg cgggttgttt
    19561 ctctagtaaa ttcaaaagat catagacaaa cggcgttgcg cctagcatac aggtgcaacg
    19621 ctgctgctca agcagcgcga gacacgcatc aggagtgaaa atatctaaca acacgctgcg
    19681 agcgccaatt aagaatggtg ccgttacgcc atgcagaaag cccgttgcgt gaccaagtgg
    19741 cgcaggcatc ataaagacat ccagccaggt cagattcagc cgcgcgcaat aagcccgctc
    19801 actggcgaga atattgttat gcgttagcat cacgcccttt ggcagaccct cggttccgga
    19861 ggtaaacagc accgcagcta attcatcgcc gtgggtcgtt atcgccgtgg tcagtgggat
    19921 attgtcggcg ataatctgac ttaatgagag ggaagaggtg gcgggagcca gtttgtccac
    19981 gccgacaatt tgttgtagtt gtggaagctg attttgcagc ggcaggatta aatctaccgg
    20041 acgcgtttgt ttaaacaacg tcggtgcaaa gaacattttt gcctgacact tattgagcac
    20101 ccacaccagt tctgcttccc gccaggaagg caacagcggc acggaaaccg caccgatttt
    20161 caggcaggca agatagataa cggtaaattc acaccagcca ggcagttgaa atgcgatgcg
    20221 atcgcctgat tcaataccct tcgctaacat ccagtttgcc agacagctcg cggcgtgatc
    20281 gagcgcgcta taggtgtacg atgcaccatg attatcgacc acggcaattt tgtctggcat
    20341 cgcacgagcg gtctgctgcc agtaatcggc cagtgaagca tcgccccata gcccttgctg
    20401 acgatacgcc gcacgacgtt gttcgttaaa cgttaatgtc actttcattt tggactctcg
    20461 aaacagaaca tcaggcccga gttgcgggcc tgtgggatgc atgattagcc gtagcgaaaa
    20521 tcaataccga aggtgcctgc gggatattgc cactgttcga gaatagtgtt accgcacagc
    20581 acccgacagg tgccgcactc cagacaaccg gcgggatcaa aatgaatgtt tcctacgtca
    20641 tcctgcttat aaagtccggc agggcaggct ttcattaatt tacggaattc attgatatcg
    20701 ggattttccg tcagaatgat atgcgggtgg ccctcatcaa catggaattt attgacgcct
    20761 aatttgatgt cgacgttaac cgtagcgttc tggctcatag cgcggttgct cccttaatgc
    20821 catctttcag caagttgatc agcccaattt tcttcgcgtg tcccatgatc attttgcgta
    20881 ccggctggtt tggtttgccg tcaatggtga acatctcgtt catgatgtcg gctaccattc
    20941 gtgggtattg gctaaacagg cgcgggtttt ccatcagcgc cgggatcttg cgaaaatgct
    21001 gcatatcacg catgacgcag ctttgttcca gctcacgttt gtattgcgcc agactgttgg
    21061 cggagaaatc cgcgcgttct ttggcggcga tcaccgttgt ggcggcagcc tgagccgatg
    21121 caatggctaa atccatgccg cggaccgtaa aacccaaatt caggcagaag cctgcggcgt
    21181 caccaacgat catcacgccc tcgttaacca gttgcggcac cattgccaga ccgccttccg
    21241 gcaccatatg cgcggaatat tcaagcagtt tgccgccgct aatcagcggg cgaatggcgg
    21301 ggtgttgttt aaaatcttcc agcatttgcg gcacgctttt ttgcgcatgg gcgatatcac
    21361 ccaatccaca aaccagcccc aaggatatgg aatccttgtt ggtatagaga aatcccccgc
    21421 ccatcaggcc gtcagaaggg gaaccggcaa acagccaggc ggcaccttcc tcgcccgtaa
    21481 tattaaagcg atcgttgatc tgttctggtg tgaggccaat aacctcttta acaccaacag
    21541 cgtaatgatg cggatcggaa gcgggaacca ttcccagcga gcggccaagc atcgagttaa
    21601 cgccatcagc cagaatcacc acattcgctt cgagaatatc atccccagcc tgcacgccag
    21661 tgaccttgtt tccttcacga accagcgcgt cgacgcgaac gcctgggata aactgcgcgc
    21721 ccgcctgctc ggcttgttcc atcaaccacg ggtccagacg attacgcaat acggtataag
    21781 atgcgtgttg aggaacatct ggttgctcgc ggtgaaaatc gagggtaacg gcgctttctt
    21841 cggttaagaa ggagattttc tcgcgtgtga ccttgcgttc taccggcgct gatactgcaa
    21901 agcctggaat gattgcttca agtgtgtggg cataaagacg cccgccggtc atgtttttac
    21961 atccggcact gtcgccgcgt tctatcacca ggacatccag cccggctcgt gccatgacca
    22021 gtgcggcaac gctaccagca acgcccgcac cgaccacaat ggcatcaaat ttgtcatccg
    22081 acatgcgtta tgctcccttc ctgctgcgtt tacagcggga gatgtggttg aaaggggaat
    22141 tagcggctca actggctaat cagggcaggg acgactttgt agatatcgcc caccagaccg
    22201 tagtcggcat agttgaagat tggcgcattt ttatctttat tgatggcgac aatcactttt
    22261 acgccgttgc cgccaaccat atgttggatc tgcccggaga tccccagcgt caggtagaga
    22321 tcggatttca gcaacacgcc ggagacaccg atataacgtt cacgctccat ccagttctcg
    22381 ccttcggcaa ttggacgtga acagccgact tcagcattca gcaccgccgc cagttcgtgg
    22441 accattttca ggtcatcctg cgccgccaga ccacgaccga cgccaaccac acgttttgct
    22501 ttgctcaggt ccacgctgct tgcggctttc gcacggcgtt cgcgacagag aatttcatga
    22561 cgcggagcaa cataaggtac cgtttctgtc gggcactgat gagaggtatc actggtgcac
    22621 ggttcctgaa caccgggtgc aagggtaatg atcgccagcg ggctgttgat cttttcctga
    22681 gcgaacgcta acccgccata catccggtgt tcggcgcaaa tgtgaccatc gacaatatcc
    22741 accgccgtgg catcgttcac cagcgccgca ttcagttgca cacttaaccg tgctgccaga
    22801 gctttaccac gtttcgtcgc ggccaacagc aacatagcgg ggtgtttatc tttcagcagg
    22861 gcagcaatgc tttcggcgta attttcagtg cgttgcagcg cgtcgttttg ctcaagaaca
    22921 taaatacatt ttggaccata aggcataact gcctgcgcct ggtcggtatt ttgtacaatg
    22981 gcatacacct gttggcccca ttgctgagcg ccgccaaaca gttcagcata acgttcagga
    23041 ttatcgctaa agacccagac gctgtttaat tgactcatcg tttcttctcc gtaggcttca
    23101 gttcagggct ttcttcagat gttccgccag ctcggcaatg gcctccggcg aatcgttatc
    23161 gagaatgatg tgcttacgtt ctgtttgcgg cggtacgcga atgccgacca gttcagcaag
    23221 tggcgcgctc tggctccagt caatatcact tgcctgccac tgatttaccg gttttttacc
    23281 cgcgccgaga atggctttca tcgaaggaat gcgtggcacg ttaatatcgg aggtgacgca
    23341 gagcacggct ggaacagaga gttcaataac ttcaacatca tcttcaagcg tgcgttcaat
    23401 caccagtgta ttgccctgac gctgaatagc actcactgca ttaatcaccg gaagttgcag
    23461 aatttctccg accagcaagc caacctgctg ggcataaagg tcgccggaac cttcaccaaa
    23521 gatcagtaaa tcgaagccga tcttttcaat tgctgccgcc agcgcttttg cggtatcgag
    23581 aggcagtgca tgttcaagtt gcgcatcctg caccaaatac aggctgtgcg gcccgcggga
    23641 tagcacgtct ttgcgcactt tcgagttctg caacaatgag ccgccaacgg tcagcgcggc
    23701 tatctcatcg tcatctgttg cgagctggct tgcagcttca atggcattga gatcgaactg
    23761 gctgattttg gcgtcggcat tgtcgaaatt cagggtgtat tctggagtga caacaatgtc
    23821 ctgttcttca ggcaccagct taaagcaggt tattattttc atgatttctc ctgtgagtcg
    23881 catacggaac catctccgat ggaaggtaaa aatggcctga aaa
//
LOCUS       DAJTRD010000060        22548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTRD010000060 DAJTRD010000000
VERSION     DAJTRD010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.060"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            26..235
                     /gene="fumD"
                     /locus_tag="OO315_002575"
     CDS             26..235
                     /gene="fumD"
                     /locus_tag="OO315_002575"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5891438.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            691..1317
                     /locus_tag="OO315_002576"
     CDS             691..1317
                     /locus_tag="OO315_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5891439.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            1338..3439
                     /locus_tag="OO315_002577"
                     /pseudo
     CDS             1338..3439
                     /locus_tag="OO315_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            3452..4090
                     /locus_tag="OO315_002578"
     CDS             3452..4090
                     /locus_tag="OO315_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5891440.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            4154..4822
                     /locus_tag="OO315_002579"
     CDS             4154..4822
                     /locus_tag="OO315_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5891441.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            4819..5604
                     /locus_tag="OO315_002580"
     CDS             4819..5604
                     /locus_tag="OO315_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5891442.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            5608..6420
                     /locus_tag="OO315_002581"
     CDS             5608..6420
                     /locus_tag="OO315_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891443.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(6432..7854)
                     /locus_tag="OO315_002582"
                     /pseudo
     CDS             complement(6432..7854)
                     /locus_tag="OO315_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(7980..8285)
                     /locus_tag="OO315_002583"
     CDS             complement(7980..8285)
                     /locus_tag="OO315_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5891444.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            complement(8393..8469)
                     /locus_tag="OO315_002584"
     tRNA            complement(8393..8469)
                     /locus_tag="OO315_002584"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8433..8435),aa:Val,seq:gac)
     gene            complement(8474..8550)
                     /locus_tag="OO315_002585"
     tRNA            complement(8474..8550)
                     /locus_tag="OO315_002585"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8514..8516),aa:Val,seq:gac)
     gene            8858..10114
                     /locus_tag="OO315_002586"
     CDS             8858..10114
                     /locus_tag="OO315_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5891445.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            complement(10155..11528)
                     /gene="mdtK"
                     /locus_tag="OO315_002587"
     CDS             complement(10155..11528)
                     /gene="mdtK"
                     /locus_tag="OO315_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5891446.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            11743..12384
                     /gene="ribE"
                     /locus_tag="OO315_002588"
     CDS             11743..12384
                     /gene="ribE"
                     /locus_tag="OO315_002588"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5891447.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            complement(12424..13572)
                     /gene="cfa"
                     /locus_tag="OO315_002589"
     CDS             complement(12424..13572)
                     /gene="cfa"
                     /locus_tag="OO315_002589"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5891448.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(13863..15074)
                     /locus_tag="OO315_002590"
     CDS             complement(13863..15074)
                     /locus_tag="OO315_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5891449.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            15187..16119
                     /locus_tag="OO315_002591"
     CDS             15187..16119
                     /locus_tag="OO315_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5891450.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            complement(16116..17141)
                     /gene="purR"
                     /locus_tag="OO315_002592"
     CDS             complement(16116..17141)
                     /gene="purR"
                     /locus_tag="OO315_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5891451.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            17440..17529
                     /gene="ynhF"
                     /locus_tag="OO315_002593"
     CDS             17440..17529
                     /gene="ynhF"
                     /locus_tag="OO315_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5891452.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            17695..18864
                     /locus_tag="OO315_002594"
     CDS             17695..18864
                     /locus_tag="OO315_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5891453.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(19010..19591)
                     /gene="sodB"
                     /locus_tag="OO315_002595"
     CDS             complement(19010..19591)
                     /gene="sodB"
                     /locus_tag="OO315_002595"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5891454.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19719..20546)
                     /gene="mepH"
                     /locus_tag="OO315_002596"
     CDS             complement(19719..20546)
                     /gene="mepH"
                     /locus_tag="OO315_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5891455.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20881..21228
                     /gene="grxD"
                     /locus_tag="OO315_002597"
     CDS             20881..21228
                     /gene="grxD"
                     /locus_tag="OO315_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5891456.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(21267..21767)
                     /gene="tnpA"
                     /locus_tag="OO315_002598"
     CDS             complement(21267..21767)
                     /gene="tnpA"
                     /locus_tag="OO315_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5891457.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            21775..>22548
                     /locus_tag="OO315_002599"
     CDS             21775..>22548
                     /locus_tag="OO315_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5891458.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVS"
BASE COUNT         5855 a         5248 c         5831 g         5614 t
ORIGIN      
        1 ttggcatgat aaataggtat aaagaatggg aaatcggaca aaagaggatg aactgtaccg
       61 agaaatgtgc agagttgtcg gtaaagttgt gctggaaatg cgcgatctgg ggcaggaacc
      121 aaagcatatt gttatcgcag gcgtattacg tacagcatta gccaataaac gcattcagcg
      181 cagtgagctg gaaaagcagg cgatggaaac cgtcattaac gcactggtaa aatgaccatt
      241 caggatgagg cggtaagctt catcctgata ttcttgcttc agttgcggtc cagttaatca
      301 ttctaaataa caactgctga aattatacga atttcggtat gtattttaat ttatagcgat
      361 aatgttattt ttattttctg caatgaacgt cacaaatgat tgtcgcttaa gttaaattta
      421 tttcaaatga taataaatat cagttcgctt ataatttctt gctttgtaat tttaattgat
      481 aacgatcaat gttaagggct cagaataatc acattatttt ttctcctaac cataaaggat
      541 tagtttatcg gcgttaaatc ttttctcata cactatcggg ttagggaaaa tatctggtgt
      601 tggggacaga gtcagcagcg tattagttga atgcttaata tcaggctgct gaattgtcag
      661 cactgtgttt gcgaagagga tgcgaaataa atgaacccgg ttgatcgtcc actattagat
      721 attggcttaa cgcgattaga attcttgcgt atatcaggaa aaggccttgc tggtctaaca
      781 attgcccctg cgttactgtc acttttgggt tgcaaacagg aagatattga tagcggcaca
      841 gtagggttga taaatacacc caaaggggtg ctggtgaccc agcgagcgcg atgcacgggt
      901 tgccaccgct gtgaaatctc ttgtaccaac ttcaacgatg gctcagtagg gacattcttc
      961 tcccgtatca aaatccatcg caattatttc tttggcgaca acggggttgg ctctggcggc
     1021 ggcctgtatg gcgatctcaa ctataccgcg gacacctgcc gtcaatgcaa agagccgcaa
     1081 tgcatgaacg tctgcccgat tggtgcgatt acctggcagc agaaagaagg ctgtattacc
     1141 gtcgatcata aacgttgtat tggctgtagc gcctgtacta cggcatgccc gtggatgatg
     1201 gccaccgtaa ataccgaaag taaaaaatcc tcgaaatgtg tgttatgcgg tgaatgcgca
     1261 aacgcctgcc caacaggggc gttaaaaatt atcgagtgga aagatattac tgtgtgaatc
     1321 ttgcaaagga aaaaatcatg gctaacggtt ggacaggtaa tatattaaga gtcaatctca
     1381 cgacaggaaa tattaccctc gaagattcca gtaagtttaa aagttttgtc ggtggcatgg
     1441 gcttcggcta caaaattatg tatgacgaag taccgctagg cacgaaacct ttcgatgaag
     1501 cgaataaatt agtctttgct accggcccat tacctggatc tggtgccccc tgtagttctc
     1561 gcgtaaatat cacctcactt tctactttta ccaaaggaaa tttagtcgcg atgcccatat
     1621 gggtggcttt tttgcagcgc aaatgaaatt cgctggatac gacgtcatta ttatcgaagg
     1681 gaaggcgaaa tcaccggtat ggctgaagat taaagatgac aaagttagcc tggaaaaagc
     1741 cgatttctta tggggaaaag ggacgcgcgc aacgacggaa gaaatttgtc gattgaccag
     1801 cccggaaacc tgtgtggcgg ctattggtca ggctggggaa aaccttattc ctctctctgg
     1861 catgttgaat agccgtaacc acagcggcgg tgcgggaact ggcgcaataa tgggttcgaa
     1921 aaacctgaaa gcgattgcgg ttgaagggac gaaaggggtc aacattgccg atcgtcagga
     1981 gatgaagcgt ctcaatgatt acatgatgac tgaacttatt ggtgcgaata acaaccatgt
     2041 cgtgccaagt acgccacaat cgtgggcaga gtattcagat cccaagtcac gctggacagc
     2101 acgtaaaggg ctgttttggg gcgcggctga aggtggtccg attgaaacgg gtgaaattcc
     2161 gccaggcaat cagaatacgg tcggctttcg tacctataaa tccgtttttg acttaggacc
     2221 ggcggcagag aaatacacag taaaaatgag cggctgccac tcttgcccga tccgttgtat
     2281 gacccaaatg aatattcctc gggtgaaaga gtttggcgtg cccagcacag gtggtaacac
     2341 ttgtgtagca aactttgtcc ataccaccat cttcccgaac gggccgaaag attttgaaga
     2401 taaagacgat ggtcgtgtga ttggtaacct ggttggtctg aatttgttcg atgactacgg
     2461 cctatggtgt aactacgggc agttgcatcg cgactttact tattgttaca gcaaaggtgt
     2521 gttcaagcgt gttctgccag ctgaagagta tgcagaaatt cgctgggatc aactggaagc
     2581 gggtgacgtt aacttcatta aagattttta ctaccgtctg gcgcatcgtg tgggtgagct
     2641 gagtcacctg gctgatggtt catatgccat cgcagaacgc tggaatttgg gtaaagagta
     2701 ctggggctac gcgaaaaata aactctggtc gccgtttggc tatccggttc accatgccaa
     2761 tgaagcgtca gcgcaggtcg gttccattgt taagtgtatg ttcaaccgtg actgcatgac
     2821 gcatacccat atcaacttta ttggttccgg cttgccattg aaactgcaac gtgaagtggc
     2881 gaaagaactt tttggttctg aagatgctta cgatgaaacc aaaaactaca cgccaatcaa
     2941 tgacgcaaaa attaaatatg caaagtggtc gctgttgcgg gtctgtttgc ataacgccgt
     3001 cactctgtgc aactgggtct ggccaatgac cgtttcgccg ctgaaaagcc gtaattaccg
     3061 gggcgatctg gcgcttgaag ccaaattctt caaagcgatc accggcgaag agatgactca
     3121 ggaaaaatta gatttagctg cagagcgtat ctttacgttg catcgtgcct acacggtaaa
     3181 accgatgcaa accaaagata tgcgtaatga acacgatctt atctgttcct gggttttcga
     3241 caaggatccg cagatcccgg tctttactga aggtactgac aaaatggatc gtgacgatat
     3301 gcatgcttcg ctcacgatgt tctacaaaga aatgggctgg gacccacagc ttggttgtcc
     3361 aacccgcgaa acattgcagc gtctggggct ggaagatatt gccgccgatc tggcagcaca
     3421 caatctactg cctgcgtaag gaatgggtaa gatgaaccat caggatgaat tgccgttagc
     3481 gaaagtcagc gaagttgacg aggcgaagcg gcaatggttg caggggatgc gtcaccccgt
     3541 tgatacggta accgaaccag aacctgctga aatcctggct gagttcattc gccagcattc
     3601 tgcggctggg cagctggtgg cgcgcaccgt gtttctctcg ccaccctatt cggttgcaga
     3661 agaggaactg tcagttttgc tggaaagcat taagcagaac ggagactacg cggatatcgc
     3721 ttgtatgacg ggaagtcagg atgattacta ttactcaacg caggccatga gtgaaaacta
     3781 cgcggcgatg tctttgcaag ttgtggagca ggacatttgt cgcgccattg cacacgccgt
     3841 tcgttttgag tgccagacgt atcctcgtcc ttacaaagtc gccatgctga tgcaagcgcc
     3901 atattacttc caggaagcgc agattgaagc cgcgatcgcc gcgatggatg ttgcacctga
     3961 atatgccgat atacggcagg tggagtcatc gacggcggtg ttgtacttgt ttagcgagcg
     4021 atttatgacc tatggcaagg cttatggctt atgcgaatgg ttcgaggtag agcagtttca
     4081 gaacccctga aatttcaggg agatgtcatg gatagggtgg acagttgcag ccactgcatt
     4141 aggggataat cagatgtcct tcactcgacg caaatttgtt ctggggatgg gaacagtaat
     4201 cttttttact ggttctgcgt cttctctgtt agcgaacacg aggcaagaaa aggaagttcg
     4261 gtatgccatg attcatgacg agtcacggtg caatggctgt aatatttgcg cccgcgcatg
     4321 ccgtaaaact aaccacgtcc ccgctcaggg aagccgttta tcgatagcac acattccagt
     4381 caccgataac gacaacgaaa cgcagtatca cttttttcgc cagtcatgtc agcactgcga
     4441 agatgctccg tgcatcgacg tatgcccgac gggggcatcg tggcgtgatg agcaggggat
     4501 cgtgcgggta gaaaaatcgc agtgcatcgg ctgcagttat tgcattggcg cttgtccgta
     4561 cctggttcgc taccttaacc ctgtcactaa agtggcagat aaatgtgatt tctgcgctga
     4621 gtcccgactg gcaaaaggct ttccgcccat ttgcgtcagc gcctgtccgg aacacgcatt
     4681 gatctttggt cgcgaagata gcccagaaat tcaggcgtgg ttgcaggata ataaatacta
     4741 tcaatatcag ctacctggtg ccggaaaacc gcatctgtat cgccggttcg gtcaacattt
     4801 gattaaaaag gaaaatgtat gaacccgtcg caacatgctg aacagtttca gagccagtta
     4861 gcgaactatg tgccacagtt cactcccgaa ttttggccgg tgtggttgat cattgccgga
     4921 gtattgctgg ttgggatgtg gttggtgctg gggctgcacg ccttgcttcg tgctcgtggc
     4981 gtgaagaaat cagccaccga tcatggtgag aaggtttatc tttacagcaa agcggtcaga
     5041 ttatggcact ggtcgaatgc gttactcttt gtattgttgc tggcaagtgg gctgataaat
     5101 cactttgcga tggtgggcgc aactgcggtt aaaagtctgg ttgcggtgca tgaagtttgc
     5161 ggatttttgt tactggcatg ctggctcggc tttgtgctga tcaatgccgt tggggataat
     5221 ggtcaccact atcgcattcg tcgtcagggg tggctggaac gagcggcaaa acaaacgcga
     5281 ttttatttgt ttggcattat gcagggggaa gaacatcctt tcccggcaac aacacagtct
     5341 aaatttaatc ccttacagca ggtcgcctat gttggtgtca tgtatggatt gctgccgttg
     5401 ttactattga cggggctgtt gtgtctctat ccgcaagccg tgggagatgt gtttcctggc
     5461 gtaagatact ggttattgca ggcgcatttt gctctggcat ttataagcct cttttttatc
     5521 ttcggtcatc tttatctttg caccacgggg cgtacgccac acgaaacctt taaaagcatg
     5581 gtcgatggct atcaccggca ctaacgaatg atcattacac gagcagacct gcgagaatgg
     5641 cgtatcggag cggtgatgta tcgctggttc ctgcgccatt tcccacgcgg cggtagttat
     5701 gccgatattc gtcatgcact gattgaggaa ggatatacgg actgggcgga gagtctggtt
     5761 gagtatgcgt ggaaaaaatg gctggcggat gaaaacttcg cgcatcagga agtttcctct
     5821 atgcaaaaac tggccacaga ccccggagag ataccatttt gcagtcagtt tgcccgcagc
     5881 gacgatcatg ctcgcatcgg ctgttgtgag gataacgccc gaatcgcaac cgctgggtat
     5941 gccgcgcaaa ttgccagtat gggttatagc gtgcggattg gaagtgtcgg ctttaacagt
     6001 cacattggca gctccggtga acgcgcacgg gttgctgtaa ccggtaattc ttcgcggata
     6061 agcagcgctg gagatagtag tcggattgcg aataccggaa tgcgagtacg cgtttgtaca
     6121 ttgggtgaac ggtgtcatgt cgccagtaat ggtgacctgg tgcaaatcgc cagctttggc
     6181 gcaaacgcca gaattgccaa cagcggcgac aatgtacaca tcattgccag cggtgaaaat
     6241 tcaacggtag tgagcacggg cgtggtcgac tcaattattc tcgggctagg gggaagcgcc
     6301 gcactggcat atcatgacgg cgaaagagtt cgcttcgccg tcgccattga aggtgaaaac
     6361 aatattcgaa ccggagtccg ctatcgtctt aatgaacagc atcagtttgt tgagtgctga
     6421 tagctttacc tttaatcaaa cgaaagacgc cgacgagcac gggatgcagg ctttacgacc
     6481 gcccgagcca tcgcctcacc aatttctgca catgccgtaa gtccctgaac gaaaggctgg
     6541 tcgtgcatca accagggcaa cgcgccgaac gctacgcgtc cacgaatatc ttcgggttgc
     6601 tgtaacgtat aatcttcgcc aacatcaggg atttcagatc cccgcttcac gtaaggccag
     6661 tagtcgacga atagactctg aagggattgc cgcataatta tcgatgaaaa ccctggcaag
     6721 gcctttactg aaccgtttat ggtcgtgttc attcagatga ggaacaattt cctgtacggc
     6781 ttcatgcagg cgcagaatga cataacgcca gggaacagta tgtttttctc gtttattgcg
     6841 ttcgacttcc tggagatttt tttctgccca gtcaaatggg tcgcgttgtt tgcgctcggc
     6901 aaaccaggct tgagcaaagg aatcgacatt caggctctct aaggctatgc gttggctcca
     6961 gtctggatca gcaaacttga tttcctctac tatcaatcta aatacccgat ccaaaaggcc
     7021 ctcttcacct ttttgaatct cagcatttaa tgcctgatcg gtgacgatgt gtaagggctc
     7081 gtagggaata gggcaataga aatcggcttc gggtaaaata cccgtgcgcg acatcaacgt
     7141 aatatttagc ttttcacttg cgttatcgcg gtgaaaaacg acgtgttgtt tatcatcttc
     7201 aatgaacgaa ccatgctgaa tagccactgc cattgccgca tccagtccgc tcaaggatgt
     7261 tcccataata cccacgttac acgcatcgac ctttgcttcc atcaggcctg accacgggct
     7321 gggaaaatac gttctggttg cttcttcttc atcaggccag acgtgacccg tggcaatcac
     7381 cgctaaatca aacgtctcgc tgggtaaatc ctgatttgta gcgagcatga cgccagcatt
     7441 tgtaatttgc agatcggtaa cctggcatga ttcataaaca gccactgcaa atttttgctg
     7501 tcgtgcctgg tctactaatc gtaaaaattg atcgcggaaa tattcgccca gcagaattcg
     7561 cggtaaaaac tgacgatcgt gcaaggtttc ttttttaacg ccataacgct ggagatggct
     7621 ggcttcttgc ttttgtagcc attcgagata cgtacaatta atcggcggta tttcaatact
     7681 ggcaatattt gccagcatca tttttgagtt ttcctcatca ctgtatggca tcccgacacc
     7741 ggcctcgtca gcctgctcga agatagaaat agaaagtgga gtttgttgct gtagaagcga
     7801 gaataaggtg tagatccccg taggcccggc acccacaata gcaatttttt tcattgttac
     7861 ttacctgtac gcattctttc gaatttagtg ttatcagata agtttggttg agatttttag
     7921 aatttgagaa atttcagaca gggatgaaaa gggcgctaac cgcccttgat taaattctgt
     7981 caggcgagtt ttgcctgatt gatttgacta agtggctcat tgacatcgaa aactttggcg
     8041 acaacttcct caacgccgag atttttcagt cgggcagtat gtttttccag ataggcaagg
     8101 gcgctttttt catcagtgaa aaggtagatc ccaccggctt cgtggttctt ttcactttct
     8161 gtccatacct tccacagaaa accaggttcc tgattaatcg actcagcaag tggtttaagc
     8221 tgctcagcca ttgcgtcgcc aaatgggcca ttaaaagcaa aatgaagttg taacaaagtt
     8281 gccatagcga ttctccttct gataacgcat acgaagacag tgtaaatctt ttaacgtatt
     8341 ggatacagag aagaaacaga gggaaaaagg aaaagaccag attaagaaaa tctggtgcgt
     8401 ccgagtggac tcgaaccacc gacccccacc atgtcaaggt ggtgctctaa ccaactgagc
     8461 tacggacgca ggatggtgcg ttcaattgga ctcgaaccaa cgacccccac catgtcaagg
     8521 tggtgctcta accaactgag ctatgaacgc aacgttgtag gtgacaacgg ggacgaatat
     8581 tagcggcaga gtggaaaggt ggcaagaggc aaaacgtaat tttctgcgct atttcgaccg
     8641 tttgcagagc ttttaagcaa attggctata ttttgttgat ttgcaagggc gatttttatt
     8701 caggatcgca tttacatctg atacaaccca ataattcgtg tatcagagtt taaacagagt
     8761 tgtttgccac catctcaaat ctgtcgtgga taaatcctcc gtagccttta cacttaggta
     8821 gcaataaagg cacacagatg cagcaaagga ataaatcatg ggatctgatg cgaaaaactt
     8881 gatgagcgac gggaatgtgc aaattgttaa gaccggcgag gtccttggcg cgacgcaact
     8941 tactgaaggc gagttgattg ttgaagctgg cggaagagcc gaaaataccg tggtcacggg
     9001 ggctggctgg ttgaaagtgg caaccggtgg gatcgccaaa tgcacacagt acggtaacaa
     9061 tggcacgcta tcggtcagcg acggtgccat tgccacagat attgttcagt ccgagggagg
     9121 cgcaattagt ctctctacgc tcgctacggt taatggccgc catcccgaag gtgaattcag
     9181 cgttgatcag ggttatgcct gcggtttgtt gctggaaaat ggcggtaacc tgcgtgtact
     9241 ggaaggacat cgcgcggaaa aaatcattct cgatcaagag ggcggcctgt tggttaatgg
     9301 gacaacctca gcggtcgtgg tagatgaagg tggtgaattg ttggtgtatc caagtggaga
     9361 agccagcaat tgtgagatta atcagggcgg cgtttttatg ctggccggga aagccagtga
     9421 tacgttgctt gctggtggca ccatgaataa tctcggtggt gaagactctg acactattgt
     9481 tgagaatgga tccatttatc gtctggggac ggatggcctt cagctctaca gttccggtaa
     9541 gacgcaaaac ctgtcggtga atgtgggggg gcgggctgaa gtgcatgccg gtacgctgga
     9601 aaatgcggta atacaaggtg gaacagtgat cctgttgtca cccaccagcg cggacgaaaa
     9661 ttttgtcgta gaggaagatc gcgcaccggt tgaactgacc gggagtgttg cattactgga
     9721 cggcgcttca atgattattg gttatggcgc agagctgcaa caatcaacga ttactgtaca
     9781 gcagggcggt gtgttgattc tcgacggcag tacggtaaaa ggtgacagcg tcactttcat
     9841 tgttggtaac atcaatctga atggcggaaa actgtggctg atcactggtg cggcaacgca
     9901 tgtgcaactg aaagtgaaac gcctgcgcgg agagggagcg atttgcctgc aaaccagtgc
     9961 gaaagaaatt tcacctgact tcatcaatgt gaaaggggaa gttaacgggg atatacgcgt
    10021 tgagataaca gatgccagtc ggcaaactct gtgtaacgct ctgaaattac agccagacga
    10081 agacgggatt ggcgcaacgc tccagcctgc gtaaaaacac aggcgcaacc cgtgaaggtt
    10141 gcgccttgtc tttattagcg ggacgctcgt tgcagaatga tggctgacgg catacgttgc
    10201 aggaaccgca tacgcagcat catcataatg gctgccgatg tcaggccgat aataaagcct
    10261 atccagaagc ctgctggccc cataggttca acgaccagat cggtcagtgc cagaatatag
    10321 ccgcttggca agcccagcac ccagtaagct gtaaaggtga tatagaaaat ggaacgcgta
    10381 tctttataac cacgcaaaat cccactgcca atcacctgga ttgagtcaga aatctgatat
    10441 accgccgcca gcaacatcaa atgcgcagcc agcgttacaa cctcgggatt gtcgttgtac
    10501 aacagggcga tttgctcccg cagtgaaacc gtgaaaatgg ccgtcagggt tgccatacag
    10561 acacccacca taagcccggt ccgcgcagcg gtttgcgcat ccagcgttga gccctgaccc
    10621 agacgataac ctacgcggat agttaccgct gccgccagcg acattggaag cacaaacatc
    10681 agtgaactaa agttcagggc aatctggtgt cctgcgacat caacaatacc gagcggagac
    10741 actaacagag ccacgacggc aaacagtgtc acttcaaaga acagtgccag cgcaatcggc
    10801 aaaccgagtt gaatcagtcg tttcataacc gcaggctcgg gcttagcggt gcctttttcg
    10861 ttacgaatat cgcgcatgga gcgggcgcgt ttaatgtaag aaaccatggc aaggaacatg
    10921 acccaataca ccgccgcagt agccacgcca caaccaacgc caccgagctc aggcataccg
    10981 aaatgaccat aaataaagat atagttcacc gggatgttca ccagcaggcc gataaagccc
    11041 attaccatac ccggcttggt ttttgccaga ccttcacatt ggttacgggc aacctggaag
    11101 aacagatatc ccggcgcgcc ccacaacaac gcacgcagat aacccacggc tttgtccgcc
    11161 agagccggat cgatgttttc catgtagcgg ataatgtaac ctgcattcca cagcaccagc
    11221 ataatgagaa cggaaacaaa acctgccagc cagaaacctt gtcgcacctg atgcgcaatg
    11281 cgctcacgtc gaccggaacc atttaattgc gcgataaccg gcgttaatgc cagcagcagt
    11341 ccgtgaccaa agaggatcgc cggaagccag atagaagtac cgatagcgac cgccgccatg
    11401 tcggtggcac tatagccgcc cgccatcacg gtatcgacaa aacccatcgc agtttgggcg
    11461 atttgcgcga gaatcaccgg gattgctaat gctaataaca gacgcgcttc actgatatac
    11521 ttctgcacgt gaacaccttt tatttgtagt tatatgagag actaaaaagc cgcctgaatg
    11581 ggcagcaaaa agaagaagca ggggaaattc agtctattgt agcgaggatt tattaattct
    11641 ccagtgaaaa aatagccatt cttacccctt cactggtaag ggcgttttcc acctgctatt
    11701 gtgctgaaca gaatgtctta actgatttca ggagttgtaa gtatgtttac ggggattgta
    11761 cagggcaccg caaaactggt gtcgattgac gagaaaccaa attttcgtac gcatgtggtg
    11821 gagttacccg accacatgct ggacggcctg gaaaccggtg cttccgtggc gcataacggt
    11881 tgctgcctga ccgtgacgga aattaacggc aaccatgtca gttttgacct gatgaaagaa
    11941 acgttacgca ttaccaatct tggcgattta aaagtggggg attgggtaaa cgttgagcgt
    12001 gcggcgaaat tcagtgatga aattggcgga cacttaatgt caggtcatat tatgaccacc
    12061 gctgaagtgg cgaaaatatt aacctcggaa aataatcgcc agatctggtt taaagtccag
    12121 gatagtcagt tgatgaaata tatcctgtac aaaggattta ttggcatcga cggtattagc
    12181 ctgaccgtcg gcgaagtcac gccaacgcgt ttttgcgtcc atttaattcc ggaaacactg
    12241 gaacgcacga ctcttgggaa gaaaaaactt ggcgcacgcg tcaacattga aatcgatcca
    12301 caaactcagg cagtggtaga tacggtagaa cgtgtgctgg cggcacgaga aaatgccatg
    12361 aatcaaccag gtacagaagc ctgatctgaa ctcgcccccg aagagggggc gatagaatag
    12421 cctttagcga gcgactcgaa ggccgttttc aacaccgcgt gagaacacca cctgccagag
    12481 ctgaatatca cgggcgcgga aagcacctgc gcaggcattc agataatagg taaacatccg
    12541 tttaaagcgt tcactgtagt tatccgcaat ttctggccat gcggcgagga atcgttcata
    12601 ccacgccatc aacgtagtat cgtaatcagc accgaagtta tgccagtctt ccatcacaaa
    12661 gtggggttcg ctggattgag caatctggcg tacagagggc aggcaaccgt tcggaaaaat
    12721 atatttatta atccagggat caacattcag atcggttttt ttcgaaccga tagtatggag
    12781 caggaatata ccttccggtt tcaaattacg atccaccacc gcaaaatagg tatcgtaatt
    12841 tttcggtccg acgtgctcga acatccccac agaaacaata cgatcaaact ggtcgttcag
    12901 gtcacgataa tcttgcagca aaatggtgac atccaggcct ttacagcgtt cctgagccat
    12961 tttttgctgt tcggcagaaa tggtgacgcc gaccacgctt acgtcatagt tagatgccat
    13021 gtagtgcgct agtccgcccc agccgcagcc aatatccagt acgcgcatcc ctggttttaa
    13081 ctgcaatttt tcacaaatca ttttgagctt cgcctgctgg gcagattcca gattatcggc
    13141 atctttccag taagcgcagg aatattgcat gaagggatca agcatgcggc tgaacaagtc
    13201 attacccaaa tcgtaatgct ccttgccgac tatccaggca cgttttttac tctgcagatt
    13261 gaagagacga gcgccggcaa tacgcagcgt gtctttgaaa tgatggggga gttggttctc
    13321 gagacctgcg cgtaagactt tgctaaaaaa catatccagt cggtcacatt cccaccagcc
    13381 atccatataa ctttcgccta accccaaaga gccttcctgc agaacgcgtt taaaaaaatc
    13441 ggggtttttc acacgaatat ccgccggggc agaaccgtta atggctatac cggcacggct
    13501 aagtaattcg ttggcgatac ggtaccagtt gtcatccggt acactgactt cttctataca
    13561 cgatgaactc atagtttctc catcactggt cggtgatcag aaccttaaaa cagcgtagac
    13621 gctttttggg ctttgtgaga aatctcgcag aaaaaaccgc gagtctataa tccgacgtag
    13681 aacagaggga aaggaggaac ccttgccgaa atgaccatcc atagtgagtc gggaacgatc
    13741 ctgtccccgt aaaaacataa tatttatcgt agctttaaca actcgaaatt agtataggcc
    13801 gctaatttca atcatacaac aaaattttgt tagcagccta agtataagta tatcgatata
    13861 gatcagtgtg attcgctatg agcgacttcg gcattgccat gattcgggca gtcaacttct
    13921 tcacaacgtt gcatcatgta acccagcgcg accagcacta ctgttgataa catcacgctg
    13981 gtggtggtga gcaatggcgt gctgatacta ataagccagg aaactaccag acttgcgagg
    14041 aagcacagac ccagttgcag agtgttctgc aacgctgcgg cgcgaccggt tgcatgtggg
    14101 aagggacgca gcgcctgggc gacaacaata gggtagatcg cgccattggc aatcgccatc
    14161 acacagaatg ggatcaggat ttcgaccagc gacacatggc taatgaagcc cgcagcccag
    14221 gtcgcaatga cgctgacagc aaacagcacc agcaaccacg gtaataactg cttgccttgc
    14281 catttctgca gtgcggcgcg acagccataa ccaccaatca gaaacgcgat agtttgcggg
    14341 acataactta aaccaataac tgccgggctg tagcccattt cactaaggat gaacggtgaa
    14401 ccggtcagcc atgcaaaaaa actggctgaa caggctgcgt atatcagcac gttgccgcga
    14461 taggttttag aacgtagcag gtcggtaaag gtcagaccat cctgactatt gttacgggcc
    14521 ttcgtcgtgg gtttgagcca gaaaataggc agaatcagca ccacggtaat ggcaaacagg
    14581 gtggcgaaaa tcgcctgcca ggaaaaatgg accagcagcc agcttcctaa caggggagcc
    14641 agtgccggag acagacccac cagcggcatg atggtcgcaa aaatacggtt gactttctgt
    14701 gaaggataat aatctgtcac taatgcttgc cagataaccg ccgcggcgca gacccccaca
    14761 gcctgtacaa aacgcaatac cagcagcgtg gcggcgtttt ctacccacag catccccaga
    14821 ctacctaacg caaaaattgt caggccgatt aataacaccg gtttacgacc ataacggtcg
    14881 gagagtggcc cccacagaag ctgggctgcg gcaaaaccgg caaggaacag actaaggctg
    14941 gcactgacag cagacgcagg cgtttgcagg tcggcctgta tggcggcgaa agcaggcaga
    15001 tacatatcgg ttgccagaaa accgagtacg ctcaaacccg ccagccagac taaaaatctt
    15061 ttcccaggtt gcatgtatat tctctaaatc tctcaattaa acaccgccgc aaagtgtagg
    15121 gagtgaaatt agtgttgtga aacggtaata tttgatgact ggtttcaaaa attttgcagg
    15181 cagactatgt ggtcagaata ttcactcgaa gttgttgatg cggtagcgcg taatggtagt
    15241 tttagcgctg cggcacagga gctgcatcgt gttccttctg cggtcagcta taccgtgcgt
    15301 cagctggaag agtggctggc ggtaccgctc tttgaacgac ggcaccgtga tgtggagctg
    15361 accgctgctg gagcgtggtt tctcaaagaa gggcgctctg ttgtcaaaaa aatgcagatc
    15421 acccgccagc aatgtcagca gatagcgaac ggctggcgcg gtcagttagc tatcgcagtg
    15481 gataatattg tcaggccaga acgtacacgg cagatgatcg ttgattttta tcgccatttt
    15541 gatgatgtcg aacttcttgt ctttcaggaa gtgttcaacg gtgtctggga tgcgctttcc
    15601 gacgggcgcg tggaactggc aattggcgca acacgcgcga ttccggtagg cggtcgttat
    15661 gccttccggg atatggggat gctaagctgg agttgcgttg ttgccagtca ccacccgctg
    15721 gcgttgatgg atggcccgtt cagcgatgat acgttgcgca actggccgtc gttggtgcgc
    15781 gaagacacct cgcgaacgct acctaaacgt attacctggt tgctggataa ccaaaaaaga
    15841 cttgtcgtac ctgactggga atcatcggca acctgtatct cggcaggatt atgcataggg
    15901 atggtgccaa cacatttcgc caaaccgtgg cttaatgagg ggaagtgggt agcacttgaa
    15961 ctggaaaacc ccttcccgga ttcggcatgt tgtctgacat ggcagcaaaa tgatatgtcg
    16021 cctgcgttaa cctggctgct tgagtatctc agcgatagtg agacgctgaa taaggagtgg
    16081 ctgcgggagc cggaagagac tcccgcaacg ggtgattaac gacgatagtc gcggaacggg
    16141 ccgtcagcca cggagcggcg ttcaatcaaa cgcggatgca cttcaatgga ctgcggttct
    16201 tcacgtttgt tgacgatacg atccaacagc atgttgaacg ctgtttcacc cagcgaatct
    16261 tttggctgat ggatcgtggt cagcgccggc gtaaaatagc gcgcgttgcg cacgttatca
    16321 taaccgatca gcgaaacatc ctgcgggacg cgcaggccca tttcatcagc agcacaaagt
    16381 gcgcccattg ccatgatatc gccaccacag aagacggcag tagggcgatg cggctgcgac
    16441 aggatttgct gcatggcgcg ataaccggat tcaggttcaa agtcaccctg cacaatccag
    16501 ctttccggca ccttgatcat cgcttcttcc atcgccttca taaaaccggc aaggcggcct
    16561 gcgccggtgt tacgttccag cgggccgggg atgacgccga tttcgcggtg accgcgttca
    16621 atcagataac gcccggccat gtagccgcct tcgaacgcgt tatcaatgac cgcatcggtg
    16681 aagtcagctt ttgcttcacc ccagtccatc accaccattg ggatatggcg atactcttcc
    16741 agcatcgcca gcaacggctc tgggtactca gaacacatca ccagcagacc atcgacgcgt
    16801 ttttgcgcca tcatcgacag ataagcccgc tgtttctcaa gattgttcca cgcattaccc
    16861 agaatcaggg tgtaaccttt ctggaagcaa tttttttcaa ctgcttcaat gatctcggca
    16921 aaataggccg cttcgctgct ggtcgccagc aaaccgatag acttggtgtg gttaaccttc
    16981 aggctacgcg ccaccgcgct aggggagtag tgtaattctt taatcgctgc ccacacggcg
    17041 ttgcgcgttt cttcagcgac gaaacgtgtt ttgttgatca cgtgtgacac agttgtagtg
    17101 gaaacgtttg ctcgtttcgc tacatctttt attgttgcca ttccatttca ctccagaccc
    17161 taacttcagc tcctgcaaaa tcgcaaggta aacgtttgcc tttacacacc ttacgcacaa
    17221 caaaagtgtt gcggtacgcc ggaaaaacga cacggagcgt caggaagggg tcaatggcca
    17281 gtacgcaaat agtgtaagcg tggaattttg gccgatcttg acgaaaaggg gaagtggtaa
    17341 aaacacttat cattataaat gacgggagat tttttcatca cagtgtgtaa aaatgcgtaa
    17401 tatcactttt tgtgtggtaa taaaaaagga gaaaacttga tgagcaccga ccttaaattt
    17461 tcactggtaa caacggttat cgtcctcggt ttgatcgtag ccgtgggttt gactgccgcg
    17521 ctgcactgat ttcctctcga gggagcgcgt tctccctcgc atctccttcc tgtttgttag
    17581 caaaatggca aaagtgtttt ctctttttgt tattttcatt tttttgtgat tgctgtcatg
    17641 ctttcgccac tttaatgaat atcgcgtaat gacgcgaaga ggtggagata aggcatgaaa
    17701 attaactatc cgttgctggc gctggcgatt ggcgcgtttg gtatcgggac aacggagttc
    17761 tcgccaatgg gcttgttgcc cgtcattgcg cgcggtgtgg atgtctcgat tcccgctgcc
    17821 ggaatgttaa tcagtgccta tgcagttggc gtaatggtgg gcgcgccgct gatgacgctt
    17881 ctactttctc atcgtgcccg ccgcagtgcg ttgattttcc tgatggcaat tttcacgcta
    17941 ggcaacgttc tttccgccat cgcgccggat tatatgaccc tgatgctttc acgtattttg
    18001 accagcctga atcacggagc attttttggt ttgggttcag tcgtggccgc aagcgtggtg
    18061 ccaaaacata aacaggccag cgcagttgcc actatgttta tggggttaac cctggcaaat
    18121 atcggtggcg tgccggcggc gacctggttg ggtgaaacca tcggctggcg gatgtcattt
    18181 ctggcaacgg cggggctggg agtgatttca atggtaagtc tgttcttctc attacctaaa
    18241 ggtggtacag gggcacgacc tgaagtgaaa aaagagctgg cggtattaat gcgtccgcag
    18301 gtgctgtctg cattgctgac gacggtactg ggagctggtg caatgtttac cctctacacc
    18361 tatatctctc cggtactgca aagtattacc cacgcaacac cggtgttcgt cacggcaatg
    18421 ctggtgctga ttggtgtcgg attctctatc ggtaactatc tcggcggcaa actggcagat
    18481 cgttcagtta acggcacgtt gaaaggcttt ttgttgctgc tgatggtgat tatgctggca
    18541 atcccgttcc tggcccgcaa tgagttcggc gcagctatta gcatggtggt gtggggcgca
    18601 gcaacctttg cggttgtacc tccgttacag atgcgcgtga tgcatgtcgc cagtgaagcg
    18661 ccaggtctgt cttcatcagt caatattggt gcctttaatc ttggaaatgc gctgggagca
    18721 gctgctggtg gtgcggtaat ttccgctggg ctgggataca gctttgtgcc ggtgacgggg
    18781 gcgattgtcg cgggactggc attattgctg gtgtttatgt cagccagaaa acaacctgaa
    18841 acagtttgcg ttgctaacag ctaattaaac aaaaacgcag aaggataacc ttctgcgtta
    18901 cagttgctga caacgtgcga tttatcttgc cgaatgcgct gttaacgcct catccgcgtt
    18961 ggcatggtaa gcgaagcgca tcaggcaatg ctgcatttgc catcagttat tatgcagcga
    19021 gatttttcgc tacgaattcc cagttcacca gcgcccagaa gtgctccaga tagccaggac
    19081 gtgcattgcg atagtcgatg taataagcgt gttcccagac atcaacggtc agcagcggag
    19141 tcgcatcggt ggtcagcgga gtacccgcgt tagaggttga aacgatagcc agtttgccat
    19201 cgctgttttt caccagccag gtccagccag aaccaaagtt tttgatcgct gcatcagtaa
    19261 actgcgcttt gaaatcggca aagctgccaa aagatgcggc gatagcttca gcgacttttc
    19321 cagtcggttc gccaccggcg ttcggtgcca ggcagttcca gtagaaagta tggttccaga
    19381 cctgagctgc gttgttgaat acgccacctt cagagctgcg aataatctct tccagtgatt
    19441 taccttcaaa cgcggtacct ttaatcaggt tgttcaggtt agtgacataa gtctgatgat
    19501 gcttgccgta gtgatactcg atagtttccg cagaaatgtg cggtgccaga gcatctttag
    19561 catatggtag tgcaggtaat tcgaatgaca ttgctactct cctttattat taatttgcat
    19621 acgtacaata gccttattgt gcgtatggat agggtagcaa attaaaaggt gagacaaaag
    19681 atgaacttac cctgttgccg taacaacagg gtaaagtttt agcgaagtgt ttttggggtc
    19741 attacccgac gagcgccaac atagtggcgc tgccagtagt cttcactgag agaagtgatt
    19801 tgaatttcct gacctgtgcg cggtgactga ataaatttgc cgttgccgac atacacgccg
    19861 acatgatcgg ctgtaccgcg tccctgagta cggaaaaaga ccaggtcgcc gtttttcagc
    19921 tcactacgtt cgataggtcc tgcatcacgc aggtgataca tttcattcgc cgtacgcgga
    19981 atacgaattt tcaccaaatc tttataagcg taataaacca ggccgctgca atcaaaaccg
    20041 gtacgcggtg agctgccgcc ccaacgatat ggcttaccaa tttgctgcat cagtttattc
    20101 attgccactt tcgtggcttt ttgcaccttc gctttgtgcg cgtcggcaag ggttcctgac
    20161 gcggcatttt tgactttcac acaatgcgat ttatagccct tacgtttggt acacttttcg
    20221 gtaaagctgg ctgaggccgt tttattgacg gcgtgtttgc tgcgagtttt ggcgttttta
    20281 gaagcggtaa tggaagattt tttaacagaa gaagcggttt tgcttttggt catactttta
    20341 ctggcggctt ttttcgctgt tttctggctg gctttctggg tctttttggt ggttgccgtg
    20401 cttttctttt tatctgcttt tttggtgata tgagtggtag cagaactctc cctggcttgc
    20461 tttgaagcat gggccatagg cgttaaaggc agggtggtaa aaagtaaagc acagagcgtg
    20521 atcgaaatac ggtttatccg cgccactgag cgtccctctg ttaaggcagg tctatcatgc
    20581 ctgcgtctga tttacaaaac ttgatgattc taatgcatag aagaagagaa agttaatagc
    20641 ttttattgtt aaaaaatacg tttcgttggc gagcgcaaaa aaatgcagca gtgaaagcta
    20701 aaaatggtta taaatacgcc agtttgccgc gcatcagaga agaaacgaat atttgaaatg
    20761 caacaaatag agcaaaggga taaaatagca aaagcgtgat gaaaatgtta ttttccgatg
    20821 ccgcactgac ccgctacaat gtcagattac tgccgtaaag aagtttgagg aagcaagaca
    20881 atgagcacca ctatcgaaaa aatccaacgc cagattgctg aaaacccgat cctgctgtac
    20941 atgaaaggtt caccgaaact gccgagctgc ggtttctctg cccaggcagt ccaggcgctt
    21001 gccgcatgtg gcgaacgttt tgcctatgtt gatattctgc agaatccgga cattcgtgcg
    21061 gaactgccga aatatgctaa ctggccgacc ttcccgcaac tgtgggttga cggcgagctg
    21121 gtcggcggtt gtgatatcgt gatcgaaatg tatcagcgtg gcgaactgca gcagctgatc
    21181 aaagaaactg ccgctaaata caagtctgaa gagccggacg cggaataatc cgtcctgcaa
    21241 tactgacgga tgcgaacggg catccgtcag tcctttatcc ggtgcgccgt aaaacctcgt
    21301 ccttcagggc ggggatataa ggcgcggttt tccacctgac caggtgtttg ctgttgttca
    21361 atgtactggc ggatgacgga tattggcgca ccttcgcagc tactggcaaa atagccagga
    21421 ctccacaaaa cgcctttgta gtaatacctg actgcaatat ctggtcgatc tcgtcgcagt
    21481 aacctattcg atatgccttt gaggctgttt accagactgg atatcgccag tttgggagga
    21541 tagttaatta acaaatggac gtgatctggt tcgccatcca tttcaaccag ttcagctgca
    21601 aaatcagcac atacatttga aaagtaagtg cgtagttttt ctgtcgtgtc atggtcaaaa
    21661 atctggcgtc ggtatttggt gacaaagacc aggtgaacat gcatcaggaa aacacaatgc
    21721 cttccacgcc ggatatcggt ttcttttttc acagaccaaa gtatcatttg acctgtgaaa
    21781 cgattacagg catttaaatt ccagttaaga cccggtggtc aacaggagcg tgaaatgagg
    21841 cgcttcgcag gcgcatgtcg tttcgttttc aatcgtgctc tggcgcttca gaatgagaat
    21901 catgaggccg gtaataaata catcccttac gggaaaatgg cttcctggct ggttgagtgg
    21961 aaaaacgcca ctgaaacgca atggcttaaa gattcgccct cacagccatt gcaacagtca
    22021 ctgaaagacc ttgagcgggc ttacaaaaac ttcttccaga agcgggctgc ttttccccga
    22081 ttcaaaaagc ggggacagaa tgctgcattc cgctacccgc aggacgttaa gctcgatcag
    22141 gaaaacagcc gtatttttct gccgaaactt ggctggatgc gctaccggaa tagccgtcag
    22201 gtcacgggag ttgtgaaaaa tgtcactgtc agccagtcct gcggtacgtg gtacatcagt
    22261 attcagacag aaagtgaagt atcaactccg gttcaccctt cagcatcaat gatcgggctg
    22321 gatgctggcg tggctaaact cgccacgctg tcagatggca cagtctttga gcctgtaaac
    22381 agttttcaaa aaaaccagaa gacgctggcg agacttcagc gacagttaag ccggaaggtc
    22441 aaattcagca acaactggca gaagcagaaa cgcaaaatac agcgactgca ttcctgtatc
    22501 gcaaatatcc gcagggacta ccttcacaaa gtcacaacga ccgtcagc
//
LOCUS       DAJTRD010000061        22410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTRD010000061 DAJTRD010000000
VERSION     DAJTRD010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.061"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            83..2683
                     /locus_tag="OO315_002600"
     CDS             83..2683
                     /locus_tag="OO315_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5891459.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2674..3657
                     /locus_tag="OO315_002601"
     CDS             2674..3657
                     /locus_tag="OO315_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5891460.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3755..4295
                     /locus_tag="OO315_002602"
                     /pseudo
     CDS             3755..4295
                     /locus_tag="OO315_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4303..4818
                     /locus_tag="OO315_002603"
     CDS             4303..4818
                     /locus_tag="OO315_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5891461.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4784..5521
                     /locus_tag="OO315_002604"
     CDS             4784..5521
                     /locus_tag="OO315_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5891462.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5632..6642
                     /gene="pyrD"
                     /locus_tag="OO315_002605"
     CDS             5632..6642
                     /gene="pyrD"
                     /locus_tag="OO315_002605"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5891463.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6816..7358
                     /gene="zapC"
                     /locus_tag="OO315_002606"
     CDS             6816..7358
                     /gene="zapC"
                     /locus_tag="OO315_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5891464.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7355..8464)
                     /gene="ycbX"
                     /locus_tag="OO315_002607"
     CDS             complement(7355..8464)
                     /gene="ycbX"
                     /locus_tag="OO315_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5891465.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8708..10816
                     /gene="rlmKL"
                     /locus_tag="OO315_002608"
     CDS             8708..10816
                     /gene="rlmKL"
                     /locus_tag="OO315_002608"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5891466.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10828..12735
                     /locus_tag="OO315_002609"
     CDS             10828..12735
                     /locus_tag="OO315_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5891467.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12865..14118
                     /gene="pqiA"
                     /locus_tag="OO315_002610"
     CDS             12865..14118
                     /gene="pqiA"
                     /locus_tag="OO315_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5891468.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14123..15763
                     /gene="pqiB"
                     /locus_tag="OO315_002611"
     CDS             14123..15763
                     /gene="pqiB"
                     /locus_tag="OO315_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5891469.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15760..16323
                     /gene="pqiC"
                     /locus_tag="OO315_002612"
     CDS             15760..16323
                     /gene="pqiC"
                     /locus_tag="OO315_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5891470.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16579..16746
                     /gene="rmf"
                     /locus_tag="OO315_002613"
     CDS             16579..16746
                     /gene="rmf"
                     /locus_tag="OO315_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5891471.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16816..17334)
                     /gene="fabA"
                     /locus_tag="OO315_002614"
     CDS             complement(16816..17334)
                     /gene="fabA"
                     /locus_tag="OO315_002614"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5891472.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17403..19163)
                     /locus_tag="OO315_002615"
     CDS             complement(17403..19163)
                     /locus_tag="OO315_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5891473.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19348..19800
                     /gene="matP"
                     /locus_tag="OO315_002616"
     CDS             19348..19800
                     /gene="matP"
                     /locus_tag="OO315_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5891474.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19876..20922)
                     /gene="ompA"
                     /locus_tag="OO315_002617"
     CDS             complement(19876..20922)
                     /gene="ompA"
                     /locus_tag="OO315_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5891475.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21278..21787)
                     /gene="sulA"
                     /locus_tag="OO315_002618"
     CDS             complement(21278..21787)
                     /gene="sulA"
                     /locus_tag="OO315_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5891476.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22006..>22410
                     /gene="sxy"
                     /locus_tag="OO315_002619"
     CDS             22006..>22410
                     /gene="sxy"
                     /locus_tag="OO315_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5891477.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGE"
BASE COUNT         5721 a         5258 c         5872 g         5559 t
ORIGIN      
        1 gtatcgaacc atcaatgact acggggcgga aacgccgcaa ctcaactgta aatcgtaagc
       61 cgtcttcagt taagagagcg agatgtatag aactcatcga caacacagcc tgttaagctc
      121 tggtggagtg ccatcgttta ttggtgggct ggtggtgttt gtgtcggcag cgttcaatgc
      181 acaagctgaa acctggttcg atcctgcttt tttcaaagat gatccctcaa tggtggccga
      241 tttgtctcgt ttcgaaaaag gacaaaaaat aacgccaggg gtttatcgtg tcgatattgt
      301 tctgaatcag acaattgtag atacgcgcaa cgtcaatttt gttgagataa cgccagagaa
      361 ggggattgcc gcctgtttga cgactgaaag cctggatgca atgggtgtta atactgatgc
      421 gtttccggct tttaaacaac tggacaaaca agtgtgtgtg ccattggcgg agattattcc
      481 ggatgccagc gtaacgttta atgtgaataa actccgtctg gaaatttcag taccgcaaat
      541 cgccatcaaa agtaatgccc gtggttatgt cccccctgaa cgttgggatg aagggatcaa
      601 cgcgctatta ctgggatatt cgtttagcgg ggctaacagt attcatagca gcgcagacag
      661 tgattctggc gacagctatt ttctgaattt aaacagtggc gttaatttag gcccatggag
      721 attacgcaac aattcaacat ggagtcgcag tagtggccaa accgcagaat ggaagaatct
      781 cagcagctat ttgcagcggg cggttattcc actaaaaggc gaactgaccg taggtgatga
      841 ttatactgca ggcgattttt tcgatagtgt cagctttcgt ggtgtgcagc tggcgtcaga
      901 tgacaacatg ctgccagaca gcctgaaagg gtttgcgcct gtggtgcgtg gtatcgccaa
      961 aagcaatgcc cagataacga ttaagcaaaa tggttacacc atttaccaaa cttatgtatc
     1021 gcctggtgct tttgaaatta gtgatctcta ttccacgtcg tcgagcggtg atttgttagt
     1081 tgaaatcaaa gaagcggacg gcagcgtcaa tagctacagc gtaccgtttt ccagcgtgcc
     1141 attactccag cgtcaggggc gaatcaaata cgcggtgaca ctggcgaaat acagaaccaa
     1201 tagtaatgaa cagcaggaga gcaaatttgc ccagaccacg ttgcagtggg gcggaccgtg
     1261 gggaacgaca tggtatggtg gtggacaata tgctgaatat taccgtgccg ccatgtttgg
     1321 tctgggattt aaccttggcg atttcggagc aatttcgttc gatgcgaccc aggcgaagag
     1381 tacgctggca gaccaaagcg aacataaagg tcagtcatat cgttttctgt atgccaaaac
     1441 gctcaaccaa ttgggcacta attttcaatt gatgggctat cgctattcga cgtcgggttt
     1501 ctacaccctt tccgacacca tgtataaaca tatggatggc tacgaattta atgacggtga
     1561 tgatgaagat acgccgatgt ggtcgcgtta ttacaatttg ttttacacca aacgtggcaa
     1621 actgcaggtc aatatctccc agcaattagg cgagtacggt tcgttttatt taagtggtag
     1681 ccagcaaact tactggcata ccgatcaaca ggatcggcta ttacagtttg gctacaacac
     1741 gcaaattaaa gatctctcgc tgggggtttc ctggaactac agtaagtccc gtggtcaacc
     1801 tgatgctgat caggtgtttg cactaaattt ttccctgccg ctcaatctgt tgctccccag
     1861 aagtaatgat agctatacca ggaaaaaaaa ttacgcctgg atgacctcta acaccagtat
     1921 cgataacgaa gggcacatta cacaaaacct gggtttaacg gagacactac tcgatgacgg
     1981 taacctgagc tacagcgtgc aacagggata taacagcgag gggaaaacgg ctaatggtag
     2041 cgccagcatg gactacaaag gggcgtttgc agatgcccga gtgggctaca actacagcga
     2101 taacggcagt caacaacaac tgaactacgc tctttcaggc agtttagttg cccattcaca
     2161 gggcattacc ctggggcaat cgctggggga aactaacgtg ctgattgcag caccaggcgc
     2221 agagaatact cgtgtggcga acagcaccgg gctgaaaact gactggcgcg gatataccgt
     2281 tgtcccctat gccacttctt atcgggaaaa tcgaatcgca cttgatgcgg catcgttaaa
     2341 acgtaacgtg gatcttgaaa atgcagtagt caacgtggtt cccaccaaag gggcgttggt
     2401 tctggcggag ttcaatgccc atgcgggagc aagggtatta atgaaaacat caaagcaggg
     2461 tataccgctg cgttttggcg cgatagcgac gctggacggc atacagacta atagcggcat
     2521 tattgatgat gatggctcgc tctatatgtc cggtttgccc gctcagggag cgataactgt
     2581 acgctggggc gaagctcccg atcaaatttg tcatatcagt taccagctta ccgaacaaca
     2641 aattaactct gcgattacgc gaatggatgc catatgcaga taatctttgg agaaaaatgc
     2701 gtgttattac tacgactatt ttttgccgcc gtcttaatgc tatggtgcgc tcaaactgct
     2761 gcttatatcg ggcagtgtca taccactcag gggaatccgt atattggcgt caattttggc
     2821 gttaaaactc tggatgaaga agaaaatacg gctggagtag ttaaagacaa attttatcag
     2881 tggaacgaat cgaatgatta ttatgtttcc tgtgattgcg ataaagacaa tgtcagaagt
     2941 ggccgatggg cattcgccgc ggattcaccg ttagtctatt taggcgacaa ctggtacaaa
     3001 attaatgact atcttgccgc caaagtttta ttgcaggtta aaggcagttc tcctactgcg
     3061 gttcctttcg aaaacgtggg cacaggggca gatacacgat ggcatatttg cgatcccggc
     3121 ggtcaacgtt taggtggcca gggggctagc ggtaatagcg gtagcttttc cctgaaaata
     3181 ttgcagccgt tcgttggttc ggtcgtcatt cctcctatgg cgctggcgcg attatttgaa
     3241 tgctacaaca tacccgcagg tgattcctgc acgactactg gtacaccggt tttagtgtat
     3301 tacctgtctg gtacgatcaa ttcacttggc tcatgttccg tcaatgccgg agaaacaatc
     3361 gaggttgatc tgggcgacgt atttgcggct aactttcgtg ttgtagggca taagcctctt
     3421 ggggccagaa cggcagaact tgcaattccg gtcaggtgta acacgggaaa cgcagggtta
     3481 gttaacgtca atctgagtct gacggcaacc tcagacccca gctatcccca ggcgattaag
     3541 acgtcacgtc ctggcgtggg cgtggtggtg accgatagcc agcacaacat tatttcccct
     3601 gctggtggga cactaccgct ctctattcct gatgatgcag acagtatccg cgaatgaatg
     3661 tctatccagt cagcacgaca agcgtaccac cagaaaccgg gcgatttgaa gccacggcaa
     3721 cggtgagaat aaattttgat taacgcgtga acgtatgaag aaaaaaacga tatatcagtg
     3781 cgttattttg ttctttagcc ttcttaacat ccatgtcggg atggctgggc ctgaacaagt
     3841 cagtatgcat atttatggga atgtggtcga tcagggctgt gatgtcgcca ccaaaagtgc
     3901 attacaaaat attcatattg gtgattttaa tatcagtgat tttcaggccg cgaataccgt
     3961 aagcactgct gctgatttga atattgatat caccggttgt gccgctggta ttactggcgc
     4021 ggacgtcctt tttagcggcg aggctgacac ccttgcgccg acactgctca aactaactga
     4081 cacaggcgga agcggtggta tggcaacggg gattgccgtg cattcttgat gcgcaaagtc
     4141 agcaagaaat cccgctcaat caggtccagc ctcttacgcc cttaaaagcc ggggataaca
     4201 cactcaaata tcaacttcgt tataagtcca caaaggcggg agcaacgggc ggtaatgcga
     4261 cggcggttct ctattttgat ctggtttacc agtgaaggag tgatgttgaa acggataatc
     4321 tggattctgt tcttactggg attaacgggg ggctgtgagc tatttgccca tgatggcacg
     4381 gtcaacatta gcggatcgtt tcgccgtaat acatgcgtgc tggcacagga tagcaagcaa
     4441 atcaatgtgc agttaggcga tgtctcgctg acacgttttt ttcatgggaa ttatggcccg
     4501 gagaaatctt tcatcattaa tttgcaggat tgcggaacag atgtcagcac ggttgatgtc
     4561 accttttcag gaacccccga cggagtgcag agcgaaatgc tgtccattga aagtggtacg
     4621 gatgcagcca gtgggctggc aattgcgatt ctggatgatg cgaagatatt gattccgctt
     4681 aaccaggcga gtaaagacta cagcctacat agcggtaagg tgccattaac tttttatgcg
     4741 caattgcgac ctgtcaatag cgatgtgcag tcgggtaaag tgaatgccag cgcgacattt
     4801 gtacttcatt atgactaata cgtggaacag attagcgctc ttgattttcg ccgttttatc
     4861 gctgctggtg gcgggtgaat tgcaggcagg agtggtggtc ggcggaacgc gatttatctt
     4921 tccggcagac agagaatcga tatctatttt actgactaat accagtcagg aatcttggct
     4981 tattaacagc aaaatcaatc gcccaacgcg ttgggccggg ggggaagcgt cgacagtgcc
     5041 agcactatta ctggccgctc cgccacttat tctcctgaag cccggtacta caggcacgtt
     5101 gcgcttgctg agaacggaaa gcgacatctt gcctgtggat cgcgaaacgc tatttgagtt
     5161 aagcattgcc agcgtgccat ccggcaaagt tgaaaatcag agcgtaaaag tggcgatgcg
     5221 ctcggtattt aaactgttct ggcgacccga aggtttgccg ggcgacccgc tggaagctta
     5281 tcaacaatta cgctggacac ggaattcgca gggtgtacaa ctcactaacc caacgcctta
     5341 ctacattaac ctgattcagg tgagcgtaaa tggtaaagcg ttaagtaatg cgggagtcgt
     5401 gccgcctaaa agccagcgtc agacaaactg gtgtcaggta atagcaccgt gtcatgtcgc
     5461 ctggcggacg ataaatgatt acggcgggtt gagtgcaaag aaggagcaaa atctgccctg
     5521 aaacaggttc tgaaaacgtt tgcgtttttt ttgccgcagg tcaattccct tttggtccga
     5581 actcgcacat aatacgcccc cggtttgcac accgggaatc caggagagtt catgtactac
     5641 cccttcgttc gtaaagccct tttccagctc gatccagagc gcgctcatga gtttactttt
     5701 cagcaattac gccgcattac cggaacaccg tttgaagcac tggtgcggca gaaagtgcct
     5761 gcgaaacctg ttaactgcat gggcctgacg tttaaaaatc cgcttggtct ggcagccggt
     5821 cttgataaag acggggagtg cattgatgcg ttaggcgcga tgggatttgg atcgatcgag
     5881 atcggtaccg tcacgccacg tccacagcct ggtaatgaca agccgcgtct ctttcgtctg
     5941 gtagatgccg aaggtttgat caaccgtatg ggctttaata atcttggcgt tgataacctc
     6001 gtagagaacg taaaaaaggc ccattatgac ggcgtcttgg gtattaatat cggcaaaaat
     6061 aaagatacgc cagtggagca gggcaaagat gactatctga tttgtatgga aaaaatctat
     6121 gcctatgcgg gatatatcgc catcaatatt tcatcgccga ataccccagg attacgcacg
     6181 ctgcaatatg gtgaagcgct ggatgatctc ttaaccgcga ttaaaaataa gcaaaatgat
     6241 ttgcaagcga tgcaccataa atatgtgccg atcgcagtga agatcgcgcc ggatctttct
     6301 gaagacgaat tgatccaggt tgccgatagt ttagttcgcc ataatattga tggcgttatt
     6361 gcaaccaata ccactctcga tcgttctctg gttcagggaa tgaaaaattg cgatcaaacc
     6421 ggtggcttaa gtggtcgtcc gcttcagtta aaaagcacag aaattattcg ccgcttgtca
     6481 ctagaattaa acggtcgctt accgatcatc ggtgttggcg gcatcgactc ggttatcgct
     6541 gcgcgtgaaa agattgctgc gggtgcctca ctggtgcaaa tttattctgg ttttattttt
     6601 aaaggtccgc cgctgattaa agaaatcgtt acccatatct aattatttct tcgaattcct
     6661 ctttataaga taatcagggc tttattttca gacctggttg ttttatattc atcgttgttg
     6721 cttatttaga cattttgtac ttttattgtt gaggttatta agcgaagcga caatggattg
     6781 tgttgctgcg gttttatagg gtaggggaga ggcagatgcg aattaaacca gacgataact
     6841 ggcgttggta ttacgatgaa gagcacgatc gaatgatgct cgatttagcc aatggtatgc
     6901 tatttcgctc acgttttgcg cgcaaaatgt tgactccaga tgccttttct cccgcaggct
     6961 tttgtgttga tgatgccgcg ctctatttct cttttgaaga aaagtgccgc gattttaatt
     7021 tatctaaaga ccaaaaagcc gaactggtgc tgaatgcact ggtagcaatt cgttatctca
     7081 aaccgcaaat gccgaaaagc tggcattttg tctcccatgg tgaaatgtgg gtgcccatgc
     7141 cgggcgatgc ggcctgcgtt tggttgagcg atactcacga gcaggtcaat ttgctggtcg
     7201 ttgaatctgg cgaaaatgcc gcgctatgcc tgctggcaca accctgcgtt gttattgcgg
     7261 gtcgcgcgat gcagttgggc gacgccatta aaatcatgaa cgacaggctg aaaccgcagg
     7321 tgaatgttga cagcttcagc ctcgaacagg cagtctaacg cgtcagctta agggcagtct
     7381 tcggtacaca gctacagcaa agaatggtgc catcatcgcc cattgctgat tttttcagcg
     7441 gcgtgacttc gccttctaaa agctgaacac ggcaacttcc acaaatgccc gcgcggcaag
     7501 aataagggat acgaattccc tgattttcta attgctccag caacacttgt tggttatttc
     7561 cacgaaatgc ctgtccctgc cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt
     7621 tggcggtatc atcagcggca gctgcgccgt aaatttttgc cggagccgtt gccagaattt
     7681 ccacctcatc gccaacgcga atcacgccgc tattacgggc aattaaattc tgaccaaaat
     7741 cgacatcgcc gttatcctgg gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg
     7801 ccggatgttt ttgccctttt tctgggctga cggtggtgaa aatacagcgg ctacaaggtt
     7861 taaccacatc aaacaccaca tcaccaatgc gaatcacttt ccagctatct tcttcccagg
     7921 ctgacgcgcc ggaaaccacc agattggggc ggaattgctc catttttaca ctggccggac
     7981 aacgttgttg gagatcacgt aacgaggctt cgttagcaag aaggtaagga tagccatcag
     8041 caaatgacag aggtacagtg ttgtggcgtt tcacgcgccg ggtcatttgt ggccccaccc
     8101 agcgtaattg cacttcgcgg gagaaaaatc cacttagcca tttgttgatc gcgtctggcg
     8161 caattcgcgc ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat
     8221 cagcaaaacg aacatatgcg ctactgccat ctggtgcggt gagatgcaag ccatcatgca
     8281 caggtgaagg agtaaaccgt accatctggg gaaactggcg agcggtaata aacgtaccgt
     8341 caggttccgt gatcataaag atgcgatcga aggccagacc actgacatca gccagagcat
     8401 gtgtaagacc aatgccgcgc atcgatttaa caggatgaat aaaaagccgg gttaatgtcg
     8461 ccacggtgcg gtcctcaaat gaaaataagc cctcaacttt atgacatgac gcgcttatta
     8521 gctataatgc gcaacaattt tcttagcgcc tgtcccgtta ggctatttta tttgtcagtt
     8581 tggccctggg ttgtgctcga aatcctcacg tacttgtgta cgctccggtt tctccgcgct
     8641 ctccatgtcc agactgactg caacaattac gcctactgcg gtaggctctt agagtaaaag
     8701 tgacgatatg aattctctgt ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac
     8761 tgaactggaa aatctggggg ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa
     8821 gggcgacaca cggcttgttt accagagcct gatgtggagc cgcctggcct cgcgtattat
     8881 gttgccgctg ggcgagtgta aggtttacag cgatttagac ctctatctcg gtgttcaggc
     8941 gatcaactgg acagagatgt ttaatcctgg cgcgaccttc gctgtccact tcagtggttt
     9001 gaatgacacc atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga
     9061 tgctttcaca cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg
     9121 cgttaacgtc tggctgcata aagaaaccgc cagtatcgct cttgatctga gtggtgatgg
     9181 tttacatctg cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc
     9241 agccgctatt gtgatgcgat ccggctggca gtcaggaaca ccgctgctcg atccgatgtg
     9301 tggttccggt acgttgctga ttgaagcggc gatgctggcg accgatcgcg caccaggctt
     9361 gcaccgtggg cgttggggct ttagcggctg ggcgcagcat gatgaagcta tctggcagga
     9421 agtgaaagcg gaagcgcaaa ctcgcgcccg taaaggccta gctgagtata gctctcattt
     9481 ttacggttcg gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc
     9541 ggggattggt gaactgatca cctttgaggt gaaagatgtc gcgcaactga ccaatccgct
     9601 gccgaaaggg ccgtacggta cagtgttgag caacccgcca tacggtgaac gtctggacag
     9661 cgaaccggcg ctgattgcgc tgcatagtct gctgggccga atcatgaaaa accagttcgg
     9721 tggctggaat ctctctttgt ttagtgcctc gccggatctg ctaagctgcc tgcagctgcg
     9781 tgcagacaaa cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga aaaattacca
     9841 cgttgccgaa agcacaccag acagtaaacc ggcaatggta gcggaagatt acgccaaccg
     9901 cctgcgtaag aacctcaaaa aattcgagaa gtgggcacgc caggaaggga ttgaatgtta
     9961 ccgcctgtat gacgccgatc tgccggaata taacgttgcc gttgaccgtt atgccgactg
    10021 ggtggtggtg caggagtatg cgccgccaaa aactattgat gcgcacaaag cgcgtcagcg
    10081 tctgttcgat attatcgctg caaccatttc ggtactgggg attgcgccaa acaaactggt
    10141 gctgaaaacc cgtgaacgcc ataagggcaa aaaccagtac cagaaactgg gcgagaaggg
    10201 cgagtttctt gaagttaccg aatataacgc tcacttgtgg gtgaacctga cggattatct
    10261 cgatactggt ctgttcctcg atcaccgtat cgcccgccgt atgctcggtc agatgagcaa
    10321 aggcaaagat ttcctcaacc tgttttctta taccggcagc gctaccgtgc atgcgggatt
    10381 aggcggtgca cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg aatgggcaga
    10441 acgcaacctg cgtctgaatg gcctgactag gcgtgcgcat cgcctgattc aggccgattg
    10501 cctggcgtgg ctgcgtgagg caaatgaaca gttcgatctg atctttatcg atccgccaac
    10561 cttctctaac tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc atctggcgct
    10621 gatgaaagat ttgaaacgtc tgctgcgtgc aggtgggacg atcatgttct cgaacaacaa
    10681 acgtggcttc cgtatggatc tcgacggcct ggcaaaactg ggactgaaag cacaagaaat
    10741 tacgcaaaaa acgctctccc aggatttcgc ccgtaaccgc cagatccaca actgctggct
    10801 gattaccgca gcctgaaagg aatagtaatg tcattaatca gtatgcatgg cgcatggctg
    10861 tcgttcagcg acgcgccgct tctcgataac gcagaactgc atatcgaaga taacgaacgt
    10921 gtttgtctgg tgggccgcaa cggcgcagga aaatcgacgt taatgaaaat cctcaaccgt
    10981 gaacaagggc tggatgacgg tcgcattatt tatgagcaag atttgattgt agctcgtctg
    11041 caacaggatc cgccgcgtaa cgttgagggt agcgtttatg atttcgttgc cgaaggcatt
    11101 gaagaacaag cggaatatct gaaacgctat cacgatattt cgcgcctggt gatgaacgac
    11161 ccgagcgaga aaaatctcaa cgaactggcg aaggttcagg aacagctgga tcaccacaac
    11221 ctgtggcagc tggaaaaccg catcaacgaa gtgctggcgc aactggggtt agatcctaac
    11281 gttgcgctgt cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg
    11341 gtgagcaatc cgcgcgtatt gctgctggac gaaccgacta accatctgga tatcgaaacc
    11401 atcgactggc tggaggggtt ccttaaaacc ttcaatggga cgattatttt catctcccac
    11461 gaccgttcgt ttatccgcaa tatggcgacg cgcattgttg atctcgatcg cggcaagctg
    11521 gtgacctatc cagggaatta cgaccagtac ctgcaggaaa aagaagaagc cctgcgcgtg
    11581 gaagaactac aaaatgccga gttcgatctc aaactggcgc aggaagaagt gtggattcgc
    11641 caggggatca aagcacgccg tacccgtaat gaaggccgcg tacgcgccct gaaagcgatg
    11701 cgtcgcgaac gtggtgaacg tcgcgaagtg atgggtaccg caaagatgca ggtggaagag
    11761 gccagccgct ccggtaagat cgttttcgaa atggaagacg tttgctacca ggttaacggt
    11821 aagcaactgg tgaaagattt ttctgcccag gttctacgtg gcgacaaaat tgccctgatt
    11881 ggtccgaatg ggtgcggcaa aaccacgctg ctgaaactga tgctcggtca gcttcaagcg
    11941 gacagcgggc gtattcacgt tggcaccaaa ctggaagtgg cttatttcga tcagcaccgc
    12001 gcggaactgg atcccgataa aacggtgatg gataaccttg ccgaaggtaa gcaagaggtg
    12061 atggttaatg gcaagccacg ccacgtattg ggctatttgc aggactttct gttccatccg
    12121 aaacgggcga tgacgccagt acgtgcgttt tctggcggtg agcggaaccg cttgctgctg
    12181 gcgcgtttgt tcctcaaacc aagcaactta ttgattcttg acgaaccgac caacgatctt
    12241 gatgtcgaaa cgctggaact gctggaagaa ctgatcgaca gctatcaggg cacggtattg
    12301 ctggttagcc acgatcgtca gtttgtcgat aacaccgtta cagaatgctg gatcttcgaa
    12361 ggcggcggta aaattggtcg ttatgttggc ggttatcatg atgcccgtgg tcagcaagaa
    12421 caatatgtgg cactcaaaca gcctgcggtg aaaaaaagcg aagaagccgc cgcgccaaaa
    12481 gcagaaactg taaaacgcag cagtagcaaa ctaagctata aattgcagcg cgaactggag
    12541 cagctaccgc aattgctcga agatctggag gcgaagctgg aagccctaca gacgcaagtg
    12601 gcggatgctt ccttcttcag tcagccgcat gagcagacgc aaaaagtgct tgctgatatg
    12661 gctgctgcag agcaggagct ggagcaagcc tttgaacgct gggagtatct tgaagcgtta
    12721 aaaaatggtg gctgatcgca aaatagtcga tttaaacaga gacagctgac aacggtaaaa
    12781 ttgcctgatg aactgggttt atcaggcatg aaaaccaaca ctttgttagt catctgtaaa
    12841 atagcgcatc attaaggagt accaatgtgc gaacatcatc atgccgcgaa gcacatcctg
    12901 tgctcgcagt gtgacatgct ggtggcgtta ccgcgccttg agcatggtca gaaagcggca
    12961 tgtccccggt gtggcacaac gttaaccgtg gcgtgggatg cccctcggca gcgtcccacc
    13021 gcctatgcgt tggctgcact gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat
    13081 atgaacgttg caggagttac cagtgaaatt acattactgg aaattcccgg cgtgcttttt
    13141 tctgaggact acgccagcct cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg
    13201 ttttgtctga taaccattct gttactggtg aatcgcgcgg aattaccggt ccgtttaaaa
    13261 gagcaactgg cacgggtgct ttttcaactc aaaacctggg gaatggcgga gattttcctc
    13321 gcgggtgtgc tggtcagttt cgttaaactg atggcttacg gcagcattgg ggtaggcagc
    13381 agctttctcc cctggtgttt attttgtgtc ctgcaactgc gcgcttttca gtgcgttgat
    13441 cgtcgctggt tatgggacga catcgccccg atgccagaac tgcgccagcc gctaaaacca
    13501 ggcgtcacgg ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc aatccttcct
    13561 gctgatgaac ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg tagaaacagc
    13621 ctgcagtgga cactcgcgct gcttgtaacg tccatcatgc tgtatcttcc ggctaatatt
    13681 ttgcccatca tggtgacgga tttattaggc tcgaagatgc cgtcgacgat tctcgctggg
    13741 gtcattctgt tatggagcga aggctcttat cccgtcgctg cggtgatctt tctggccagt
    13801 attatggtgc caacgttaaa gatgatcgcc atcgcgtggc tgtgttggga tgccaaaggg
    13861 catggcaagc gcgacagtga aagaatgcat ttgatttatg aagttgttga gtttgtaggc
    13921 cgctggtcga tgattgacgt tttcgttatc gcggtgctct cggcgctggt gcgtatggga
    13981 ggtttaatga gtatttatcc ggcaatgggt gcattaatgt ttgctttagt cgtcataatg
    14041 acaatgtttt ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca acctgaatca
    14101 gagcatgagg agtcctgaca gtatggaatc taataatggg gaagccaaaa tccagaaagt
    14161 gaagaactgg tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg gggcctgggt
    14221 tcttttttat cattacagcc atcagggacc ggaagtgacc ctgatcaccg cgaatgcgga
    14281 aggaattgaa ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg gcgtggttga
    14341 aagcgccaca ctggctgatg atttgacgca cgttgaaatc aaagcgcggc tgaattccgg
    14401 tatggaaaaa ttgctgcata aagacaccgt cttttgggtg gtgaaaccgc agattggtcg
    14461 cgaagggatt agcggcctgg gaacgctgct gtctggagtt tatatcgaac tgcagccagg
    14521 cgcgaaaggc agcaaaatgg ataaatacga tttgctggac tcaccaccgt tggccccgcc
    14581 tgatgcgaaa ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc tctcgccagg
    14641 agatccggtg ctgttccgtg gctatcgggt aggttcggtt gaaaccagca ccttcgatac
    14701 gcaaaaacgc aatatcagtt atcaactgtt catcaatgca ccttatgacc gactggtgac
    14761 cagcaatgtt cgcttctgga aagatagtgg cattgcggtt gatctgacgt cagcggggat
    14821 gcgtgtggag atgggctcat tgacaacgct gcttagtggc ggtgttagct ttgatgtgcc
    14881 ggaaggtctg gatttagggc agccagtggc accgaaaaca gctttcgttt tgtatgatga
    14941 tcagaagagc attcaggatt cgttgtacac cgatcacatt gattatctga tgttctttaa
    15001 agattcggta cgcggtctgc aaccgggagc tccggtagaa ttccggggta ttcgcctggg
    15061 taccgtaagc aaagtgccat tctttgcgcc gaatatgcgt cagacattta acgatgatta
    15121 ccgtattccg gtactgattc gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa
    15181 tgcggatgtt gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa
    15241 aaccggaaac ctggtcactg gtgcactgta tgttgatctc gatttctatc caaatacgcc
    15301 tgcaataacc ggtattcgtg aatttaatgg ttatcagatt atcccgactg ttagcggcgg
    15361 cttggcgcaa atccagcaac gactgatgga agcgttggat aagatcaaca aactgccatt
    15421 gaatccgatg attgaacagg caaccagtac gctttctgaa agtcagcgca caatgaaaaa
    15481 cctgcaaacg acgctggata gcatgaacaa gatcctcgcc agccagtcga tgcagcagtt
    15541 gccgacggat atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg gcttccagcc
    15601 tggctccgca gcctacaaca agatggtggc ggatatgcag cgccttgatc aggtgttgcg
    15661 agaactgcaa ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc
    15721 gaaggacaaa aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg
    15781 attgcagcac tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa ctattaccag
    15841 ttacctgtgg tgcagagcgg tacacaaagt accgccagcc agggcaatcg tctgttatgg
    15901 gtagagcagg tcactgttcc tgactatctg gcggggaatg gtgtggttta tcaaaccagt
    15961 gatgtgaagt atgtgattgc caacaacaac ttgtgggcca gcccgttgga tcaacagttg
    16021 cgcaacaccc tggttgccaa cctgagtacg caactgcccg gctgggtggt tgcctcccag
    16081 cctctgggaa gcgcccagga cacgctcaat gttaccgtaa cggagtttaa cggtcgctat
    16141 gatggcaagg tcattgtcag tggtgagtgg ctgttgaacc accagggaca actgatcaaa
    16201 cgtccgttcc gtctggaagg agtgcaaact caggatggtt acgatgagat ggttaaagtg
    16261 ctggccggtg tctggagtca ggaagccgct tctattgcac aagagataaa gcgtctacct
    16321 taattataaa gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc ggtttttttg
    16381 tttctaacgt gcggaaaaat ttgttcctct tcacattttt tgtacaaccg acatgcccgt
    16441 gtagctcaca aatatgacag tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg
    16501 gtattgctta actgtgattg cacatttagt aatcactgtt ttcttttcca ccagaaacca
    16561 gtatgaggga aacgaggcat gaagagacaa aaacgagatc gcctggaacg ggcacatcaa
    16621 cgtggttatc aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg
    16681 agtcaaaggt cacaatggct gggaggctgg cgagaagcca tggcggacag ggtagtaatg
    16741 gcctgattct gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc cttttgatac
    16801 tctgtctgat gataatcaga aggcagacgt atcctggaac agaccgactt tcaggtcgct
    16861 ggcggtatag atcagacgac aatcaaccag cacttcgcca tctgccagac ccataatcag
    16921 acgacgatta acaatgcgtt taaagtgaat acggtaggtc acttttttcg ctgtcggcag
    16981 tacctgacca gtgaatttca cttcgccaac gcccagcgcg cggcctttac cttcgccgcc
    17041 cagccagccg aggtagaacc ctaccagctg ccacattgcg tccaggccca ggcatcccgg
    17101 cataaccgga tcgccaatga agtggcatcc gaagaaccac agatccggat tgatatccag
    17161 ttctgcttca acataccctt tgtcgaagtt accacccgtt tcggtcattt tgaccacacg
    17221 gtctatcatc agcatgttcg gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc
    17281 gtgaccagag gcaagaaggt cttcttttgt ataggattcg cgtttatcta ccatgttctc
    17341 tgtaagcctt attttattga agcacgcagg atagctaaca cgtgtacgct gaacaagtcc
    17401 gatcagttcg gaataaacca gttcagccaa cgtaatggcc atggaaaacg gtgacgtcct
    17461 tcctgttgcg atgcttgcgc gatacgttcc tggatggttt gcatcagcgt cgtttggcct
    17521 tcgccatccc acaccagatt taataataac ggcagtgcgt cagtcacatc gtctactgcc
    17581 cagatggtga atttgccttc ttctaccgct ttcaccagtt cactgtgaag acttaaatgg
    17641 cgaacgttag cagtggggat aatgacacct tgtttccccg ttaactcacg ttgctggcaa
    17701 atagcaaaga agccttcgat tttctcattt aaaccaccga ccggctggac gcggccgaac
    17761 tgatcgactg aacctgtgat agcgatactc tgattcactg gcacatcagc gagggcgctt
    17821 atcagggcgc agagttcagc catcgaggca ctatcgccat ccacttcact gtatgactgc
    17881 tcaaatgtca gcgatgctga gaaggggatc tgttgctcaa gctgtagttc cgacatcagg
    17941 aacgcctgca tgatcatcat ccctttcgca tggatattgc cgccaagctc cgctttgcgt
    18001 tcgatgtcgg tgaattcacc atcgccaata tgcacaacgc agctaatgcg agaaggttcg
    18061 ccaaaagcgc gtggatgacc cggaaattca atgaccgaaa gggcgttaat ttgcccgatg
    18121 cgttcgcctt cggtttcaat caggatttgc tcctgaagga tctcatcctg catacgttca
    18181 gcgaggaaac cttcgcgcca ttcacgctgc tgcagcatta agtttagctg ctcgccggag
    18241 aaggtgtcgc catcacacag ggaggcgacc tctttacact ggcggaggat ccactgcggg
    18301 ctaagcggaa gtgtttcttg ttcaccggtg tagcgtgctg cttcgcggat aagtaccggc
    18361 caagcatccg ctcccggggc aggtaagtga ttatgtctgg cggtaaatgt cacccagcga
    18421 caccactggc ttactgactc cgcatcgaca atctgcagag tatcttcaaa ttcgctataa
    18481 atagcctgct ctgaaagctc tggctccatc tcctggaaat cagccaatga ttcgcgttcg
    18541 cctaccagaa tgactttcag cttcaatggc attgaaggca cagagacggg gagagggcgc
    18601 gactcatcaa acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc
    18661 cacagcagag gttgcgccag cagtgtacgc aaagagatga tgagaatacc gccatttgcc
    18721 tgatgcacca gaccaggctg cagggtaatg tcgccattaa actggcgcag gcagccaaag
    18781 agttgctccg cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca
    18841 tctgcactca ctgcgtgacg taagcggatg gagtggccgg aaacctcata gtgatcgccc
    18901 accagttggc ctgcatcgct ttgtagcgta cgcgcggcat cggcaataag attcagatac
    18961 tcagactctt ccggggcctt agccagcata aaagaggagg atgctcgcgt atgcagcaac
    19021 tgctccagcg caaattgcag tcgcggttga gtatcactga ataaaggatc gttttcgtca
    19081 atcaaatgtg gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc
    19141 catgcaagtt tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt
    19201 ataccgtaac cggcactaca gcacacgtag aaagcaccga caatactcct ggctgggcgt
    19261 taaagctcac aggatggaga ttcttttctt cactggccta aaaagctgat attctgtaaa
    19321 gagttacacg gtaacattga gatcgctatg aaatatcaac aacttgaaaa tcttgaaagc
    19381 ggttggaaat ggaagtatct ggtgaagaag catcgcgaag gggagttaat tacccgttac
    19441 atagaagcca gtgccgccca ggaagccgtc gatgtgttgc tctcgctcga aaatgaaccc
    19501 gtgctggtaa atggctggat tgacaagcat atgaatccgg aactggtcaa tcgcatgaag
    19561 cagaccattc gggccagacg gaagcgccat tttaatgcgg aacaccagca tacgcgcaaa
    19621 aaatcgatcg atctggaatt tattgtctgg caacgtctgg ctggtcttgc acagcgtcgc
    19681 ggtaaaacgc tttctgaaac gattgttcag ctgattgaag atgcggaaaa caaagagaaa
    19741 tacgcgaata aaatgtcttc tcttaagcag gatctgcagg cattgctggg taaggaataa
    19801 ctgacgaaag tcagttcaat ttactaaagg caaaaaaaac cccgcagcag cggggttttt
    19861 ctaccagacg ataacttaag cctgcggctg agttacaacg tctttgatac ctttaacttc
    19921 gatctctacg cgacgatccg gagccaagca gtcgatcagt gcagcacgct gtttcacgtt
    19981 gtcacaggtg ttgccagtaa ccgggttgga ttcgcccata ccacgtgcgg agatcttgtc
    20041 tgccgggata cctttggaga tcaggtaatc aacaacagac tgagcacggc gctcggacag
    20101 accctggttg taagcgtcag aaccgatgcg gtcagtgtaa cccagaacaa ctacggaacc
    20161 gtctttcgga tctaggttgc tcagctggct gtacagctga tccagagcag cctgaccttc
    20221 cggtttcagg gttgctttgt tgaagttgaa cagaacgtca gacttcagag tgaagtgctt
    20281 ggtctgtact tccggtgccg gagctggagc aactaccgga gctgcttcgc cctgaccgaa
    20341 acggtaggaa acacccaggc tcagcagacc gttgtccgga cgagtaccga tggtgtttgc
    20401 gtcaccgatg ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc
    20461 gtactcaaca ccacctgcga aaaccggaga aacgccagtg tcgtggtctt taaaggatgc
    20521 gccaccaggt acgttagcct tggtgtctgc acgccatacc ataccaccca gacgagtgta
    20581 gatgtccaga tcgtcagtga ttgggtaacc cagtttagcg gtcagctgaa cgccctgagc
    20641 tttgtatgcg ccgttgatgt tgtcgccttt gtacggcata cgacctaacc agtcgtaacc
    20701 catttcaaaa ccaacatacg ggttaacctg gtaaccacca aaagcacctg cacccagttg
    20761 gttttcgtgg gtcggaccat tgttaggaat aaaaccagtg tcatggtact gggaccagcc
    20821 cagtttagca ccagtgtacc aggtgttatc tttcggagcg gcctgcgcta cggtagcgaa
    20881 accagccagt gccactgcaa tcgcgatagc tgtctttttc attttttgcg cctcgttatc
    20941 atccaaaata cgccatgaat atctccaacg agataacacg gttaaatcct tcaccggggg
    21001 atctgctcaa tattaactct accgatatct tcggcttatg ccgagcaccc ctggcgatgt
    21061 aaagtctaca acgtagttga aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa
    21121 agtcttgtat aaggtatgtt taatcttttt gtcagcgaca atttacagaa aaaaaacgcg
    21181 gaaaccgctt cagacaagcc ttcgcaagga aaattagtca cgactgaaag cattggctgg
    21241 gcgacaaaaa aagttctagg attaatccta aatttactta atgatacaaa ttagagtgaa
    21301 tttttagccc ggaaagttgt ctcgttgtgt gagaggatgc gcttaccgga cgcataataa
    21361 accccatagc gttaccttca tttgccgcat caacaagttc agcatgctct tcttcagtca
    21421 aatcatctgt caaccaaccg atcaccacac tgtaattgcc cgtgcgtaaa gcgcgaacca
    21481 ttgactccac tgtgtggcaa ggggagagct ggctaatctg cattactttc gttaagggta
    21541 gcccagatgc ctgaacccat tcccgactca gtttttgttg cggtgttaac cagagttgcc
    21601 agcgcgattg ctgaccgagt tgctgtaaca atggcaacag tagaagttgc gtcatcatgg
    21661 gctgatcttc gcgatagaca acttcactga taagcccggc tgtagtgttt tccgtagaga
    21721 cacgcgcaat tttacttgct gcggatgaga acgacgaaga acgatgtgca tagcctgaag
    21781 tgtacataat caatccagcc cctgtgagtt actgtatgga tgtgcagtac atccagtgac
    21841 aacaaagatc aaccctattt tcggaaagag cctcgcaaat tttgtcgttg gtgacgggaa
    21901 aacataaatt aatcttgccc cttaagaata agttgcctat tttcgtagtt aacggatccg
    21961 ttaatgtgaa tcattctttt atgttatgat tttaaaagga attttatgaa aagcctctcc
    22021 tataagcgga tctataaatc acaagaatac ctggcaacgt tgggcacaat tgaataccga
    22081 tcattgtttg gcagttacag cctgaccgtt gacgacacgg tgtttgcgat ggtttctgat
    22141 ggtgagttgt atcttcgggc ttgtgagcaa agtgcacagt actgtgtaaa acatccgcct
    22201 gtctggctga catataaaaa gtgtggccga tccgttaccc tcaattacta tcgggttgat
    22261 gaaagtctat ggcgaaatca actgaagctg gtgcgtctgt cgaaatattc tctcgatgca
    22321 gcgctgaaag agaaaagcac gcgcaatacc cgggaaagac tgaaagattt gcccaatatg
    22381 tcttttcatc tggaagcgat tctcggggag
//
LOCUS       DAJTRD010000062        22268 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTRD010000062 DAJTRD010000000
VERSION     DAJTRD010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.062"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..1015)
                     /locus_tag="OO315_002620"
     CDS             complement(<1..1015)
                     /locus_tag="OO315_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5891478.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAP"
     gene            complement(1117..2097)
                     /locus_tag="OO315_002621"
     CDS             complement(1117..2097)
                     /locus_tag="OO315_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5891479.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2105..2815)
                     /locus_tag="OO315_002622"
     CDS             complement(2105..2815)
                     /locus_tag="OO315_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5891480.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3032..4855)
                     /gene="typA"
                     /locus_tag="OO315_002623"
     CDS             complement(3032..4855)
                     /gene="typA"
                     /locus_tag="OO315_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5891481.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5228..6637
                     /gene="glnA"
                     /locus_tag="OO315_002624"
     CDS             5228..6637
                     /gene="glnA"
                     /locus_tag="OO315_002624"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5891482.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6810..7859
                     /gene="glnL"
                     /locus_tag="OO315_002625"
     CDS             6810..7859
                     /gene="glnL"
                     /locus_tag="OO315_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5891483.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7871..9280
                     /gene="glnG"
                     /locus_tag="OO315_002626"
     CDS             7871..9280
                     /gene="glnG"
                     /locus_tag="OO315_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5891484.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9392..9502
                     /locus_tag="OO315_002627"
     CDS             9392..9502
                     /locus_tag="OO315_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5891485.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9731..11104)
                     /gene="hemN"
                     /locus_tag="OO315_002628"
     CDS             complement(9731..11104)
                     /gene="hemN"
                     /locus_tag="OO315_002628"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5891486.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11293..11802)
                     /gene="yihI"
                     /locus_tag="OO315_002629"
     CDS             complement(11293..11802)
                     /gene="yihI"
                     /locus_tag="OO315_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5891487.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12384..13016
                     /locus_tag="OO315_002630"
     CDS             12384..13016
                     /locus_tag="OO315_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5891488.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13398..16184)
                     /gene="polA"
                     /locus_tag="OO315_002631"
     CDS             complement(13398..16184)
                     /gene="polA"
                     /locus_tag="OO315_002631"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5891489.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16548..17480
                     /locus_tag="OO315_002632"
     CDS             16548..17480
                     /locus_tag="OO315_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5891490.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17521..18951)
                     /locus_tag="OO315_002633"
     CDS             complement(17521..18951)
                     /locus_tag="OO315_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5891491.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19106..19732)
                     /gene="dsbA"
                     /locus_tag="OO315_002634"
     CDS             complement(19106..19732)
                     /gene="dsbA"
                     /locus_tag="OO315_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5891492.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19749..20735)
                     /gene="srkA"
                     /locus_tag="OO315_002635"
     CDS             complement(19749..20735)
                     /gene="srkA"
                     /locus_tag="OO315_002635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5891493.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20812..21081)
                     /locus_tag="OO315_002636"
     CDS             complement(20812..21081)
                     /locus_tag="OO315_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5891494.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21151..21735
                     /gene="mobA"
                     /locus_tag="OO315_002637"
     CDS             21151..21735
                     /gene="mobA"
                     /locus_tag="OO315_002637"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5891495.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21717..22244
                     /gene="mobB"
                     /locus_tag="OO315_002638"
     CDS             21717..22244
                     /gene="mobB"
                     /locus_tag="OO315_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5891496.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5512 a         5580 c         5330 g         5846 t
ORIGIN      
        1 taggtgcaaa aaatacggct cgctgtgtaa agactatcgc gccaaagccc agcgtacatg
       61 ccatccctaa atagaccggc atactttcgt gcggcagcat aatcaacagt acgagcgcag
      121 cggtactgat gataaaggtg tagcatagat atttactcgg cgatttcagg atcttatctg
      181 aaatcatgcc accaatcggt ccaccaatca ttttcagaca gtattggtta atgatgccgt
      241 aagcccccac cagcgcaacg ggcaatagat agatgttttt caggaatgga atgaagaatg
      301 tcaggccaca gtaaaccgcg taaacgaaga agacgttgaa agcgataagc cagatggttt
      361 tgtctctcag cactgaggtc atactggtgt tttgcgatgc tccgtcttct tttttcacct
      421 caacggacgg tgcctcttct ttgtcattca ggacaaagaa aataataatg cctacggcaa
      481 tcacaataag ggaatagaac cagatgcctg ctttaaaacc taataagcca ctgccaaacc
      541 aggtaaatac tgccaacgca gaaaatgcca ccacggtatc gacaatgcca cgccctgttt
      601 cgaagaagcc aaacaaccga ccttgttgtt cgctattgcc caatcgactt accgacttga
      661 gcaagaccgg ccagttcatc atgtcgcaag taacgccaaa tagcgcccag acaaagagga
      721 tgccccaata ccccggcatt gtcgtcaaat aaacacccag taatcctgtc gcaatgagtg
      781 agaatgacat ggtgtatctg cgcggtagtt tatcggcaaa ataaatagac agaaaaaagc
      841 ccactgtggt gacaaatgag tttaccgaca tagcattacc aatttgacca ttggtcaaat
      901 ggaaatattc ctgcatcggg atataaaacg catctttcag cgacggtaat ttataaattg
      961 taccgccaca cagtgttaat agactaaata acgcccattt ctttttcgtg agcataacat
     1021 cccctaagcg atttttaaaa tcactgtttt aaaggcaata agataccgac cattcatatg
     1081 gaatggtttt attaactgcg tatgagttaa aacttattag tggttcagaa gatggtttgc
     1141 ctcttgtttg atttgttgta gtacgttacg cacatgattt atctgattaa ttgcatcttc
     1201 tttttcttta cgtaaccgcg catccagtaa atgattttct ggtaggggtg tttcactcat
     1261 ctggcgataa gggatccacg gattatcatc ttcgtcttca aagcggaacg ccgacgcata
     1321 gtaatcaacc tcttcttcca ggccatatgc cgtcacctgc ggctcatcat cgccaaggca
     1381 gataaggtgc gttaataacg ctttgaatgg catatcaccc tgacctgaaa tacaggcttt
     1441 atgacccagg ccaccctgtt ctttaacgat caaggcatct ttaatatgga cctgggtaat
     1501 atgcggtgcc atcgttttta aggcgtcgat gggatgctca tttgcattga tcatgttagc
     1561 aaaatcaaac agtaaggaaa gagactccat ctcactttct ttgaccagcg acaccagctc
     1621 atgacttttt aaatcttcat gctgctcaat cgtaaaagtc aggccgctgt cctgatacgt
     1681 ttcccgtaca taggcaatgt cgttagcgat aatcgataat acgtcgcgca aattaccttc
     1741 ataacatgga taaaaacgta cggacgttgc cccagttttc aacgcgatgg cgacggcttc
     1801 gtcaatagat gccttatctg aagcgctggt ttcaatatga atatcaaggt tcagacggcg
     1861 ggctttatcc ccaaaggcag agagttcttt atcgtccata ttaccaagag aaaaacgctc
     1921 gccatccaga acatggattt tgacgccacg tagattattt tcacttgcga tatcaagtaa
     1981 atcagcaggc aacactcttt ccagccgcat atttaaatga aaggcataag catgtaaata
     2041 aagaggaaga gtgtcgacac gttgtagaat ctttcttgcg ttatttattg tcaccataaa
     2101 acacctacag attaatgatg aggtctacgg cctcaaatcg ccaatattca atatctatct
     2161 ggacaatatt gccttgctgg tcggcgcgat gcttttcaat taagatcgct ggcatacccg
     2221 cagaaccgcc aagatacttg ctgatatcgc ccggcattct gacgggtttg aaaatcaatt
     2281 tttttactac cgtctctttt tggtagcact tttcccagac tgcagaaaat gagtggtttt
     2341 caagttgttc aataaaaccc ggagcaactt caggattaat atatgtttcg tgatagaaaa
     2401 ctttatgtcc ttccagcgcc ccccagccgg tgatgcgata gagttcagtt gatggcgtca
     2461 ctgcaatcag tggcgcgagc gtctcgggaa tatcgctggt acggtttttc tcggtaaacc
     2521 cccaagaagg ctctcgccct tgctcaattg cggcccgctg aaagctcgcc gacagctccg
     2581 gactgtaatt aaatcgcggc tgggtcacga accacccctt acgatcctta cggaagattt
     2641 tggattctgc ctcgaggttc aacaacgcct ggcgcagcgt catacgttta atgcccagca
     2701 gttctcccag ctctctttct gaagggagtt ttccacccgg cgtggtgatg ccatctttca
     2761 accaccgatc cagtttctct tttgcatttt ctacggtgga ttgattctcc gccattgttt
     2821 aatccttttg gtttaaacca gatgaagcat cttacgctta cgccaaaaaa gaaagcaatt
     2881 aacggtgtat gactttgcag ataattgtga caaagctctc tattgacgta atcacagact
     2941 tataagggag atactcgcat tggtgtgcgc tcacgacagg taaaaaaaaa cctgccatcg
     3001 ctggcaggtt ttttatgact aaaaaacgaa attaatcgtc tttcggtgcg cggttggcgc
     3061 ggcgacgatc gttttccgtc aggtgacgtt tacgaatacg gatagaggtc ggagtcactt
     3121 ctaccagttc gtcatcatcg atgaactcca gagcttgttc cagagtcatg cggataggcg
     3181 gaaccagaac aacagcttcg tcagtaccgg aagcacgcat gttggtcagt ttcttaccgg
     3241 tcaggcagtt tacagtcagg tcgttagagc ggctatgaat accgataatc tgaccttcgt
     3301 aaacttctgc accgtgaccg aggaacagct taccgcgatc ctgcagaccg aacagcgcga
     3361 acgcgacagc tttaccctga ccgttagaga tcagtacacc gttctgacgc tgacccactt
     3421 cgcccggacg tacgtcgtcg tagtggctga aggtggagta cagcagacca gtaccggaag
     3481 tcatggtcat gaactcagaa cggaagccaa tcagaccacg gcttgggatc acgtagtcga
     3541 gacgtacgcg gcctttaccg tctggattca tgtttttcag gtcgccttta cgttcgccca
     3601 gcgcctgcat cacagaaccc tgatgctgtt cttcaacgtc cagcgtcacg ttttcatacg
     3661 gctcttgttt acgaccgtct atttcacgga agattacttt cggacgggat accgccagtt
     3721 cgaaaccttc acgacgcatg ttttcgataa gaacagagag gtgcagttca ccacgaccag
     3781 aaacgcggaa cgcatcggcg tcttcggttt cttctacgcg cagcgcaacg ttgtgtacca
     3841 gttctttgtt cagacgatcg aggatctgac gagacgttac gaacttacct tctttaccgc
     3901 agaacggcga ggtgttaacg cagaagaaca tagaaacggt cggctcatca acggagagtg
     3961 ccggcagcgc ttcaacgttt tgcgtgtcgc aaacggtgtc agaaatgttc agttcgccca
     4021 ggcccgtgat cgccacgata tcgccagctt ccgccagatc ggtttcgata cgttccagac
     4081 cgaggtggcc cagcacttta ccgactttcg cgttacgggt tttgccttcg ctatcgatga
     4141 tagtaacctg ctggttcggc ttcactttac cacgcttgat gcggccaatg ccgataacgc
     4201 caacatagct gttgtaatcg agctgagaaa tctgcatctg gaacggaccg tcaaggtcaa
     4261 cgtccggcgc aggaacgtgg tcaacaatcg cctgatacag cggggtcatg tcttccgcca
     4321 tatcttcgtg gtccagaccc gcgataccgt tcagcgcaga agcgtaaacg atcgggaagt
     4381 ccagctgctc gtcggtcgcg tcgaggttaa cgaacagatc gaatacctga tccacaaccc
     4441 aatcaggacg cgcgccaggg cggtcaactt tgttgataac aacaatcggc ttcaggccgt
     4501 aagcaaacgc ttttttggtt acgaagcgcg tttgcggcat cgggccgtca aatgcgtcaa
     4561 ccaccagcag cactgagtct accatggaca ttacacgttc aacttcacca ccgaagtcgg
     4621 cgtgccccgg ggtatcaacg atgttgatac ggtaatcatt ccatttgata gcggtgtttt
     4681 tcgcgaggat ggtaatccca cgctctttct ccaaatcgtt ggagtccatc acgcgctctt
     4741 gggtttcggc acgagagtcg aacgtaccgg attgttggag cagcttgtct accagggtgg
     4801 ttttaccatg gtctacgtgc gcgatgatgg cgatattacg caatttttcg atcacaactt
     4861 tgcctcaggc attagaaata gcgcgttatt gtacacggat taatcgcact acaaaacagg
     4921 atcacaaaca tcctccgcaa acaagtattg cagagtccct ttgtgatcgc tttcacggag
     4981 cataaaaagg gttatccaaa ggtcattgca ccaatatggt gcttaatgtt tccattgaag
     5041 cactatattg gtgcaacatt cacatcatgg tgcagccctt ttgcacgatg ttaagcatga
     5101 taacgccttt taggggcaat ttaaaagttg gcacagattt cgctttatct tttttacggc
     5161 gacacggcca aaataattgc agatttcgtt accacgacga caatgaccaa tccaggagag
     5221 ttaaagtatg tccgctgaac acgtactgac gatgctgaac gagcacgaag tgaagtttgt
     5281 tgatttgcgc ttcaccgata ccaaaggtaa agaacaacac gtcactatcc ctgctcatca
     5341 ggtgaatgct gaattcttcg aagaaggcaa aatgtttgac ggctcctcga ttggcggctg
     5401 gaaaggcatt aacgaatccg acatggtgct gatgccagac gcatccactg cagtgattga
     5461 cccgttcttc gccgactcca ccctgattat ccgttgcgac atccttgaac ctggcaccct
     5521 gcaaggctat gaccgtgacc cgcgctctat tgcgaagcgc gccgaagatt acctgcgttc
     5581 cactggcatt gccgacaccg tactgttcgg gccagaacct gaattcttcc tgttcgatga
     5641 catccgtttc ggatcatcta tctccggttc ccacgttgct atcgacgata tcgaaggcgc
     5701 atggaactcc tccacccaat acgaaggtgg taacaaaggt caccgtccgg cagtgaaagg
     5761 cggttacttc ccggttccgc cggtagactc tgctcaggat attcgttctg aaatgtgtct
     5821 ggtgatggaa cagatgggtc tggtggttga agcccatcac cacgaagtag cgactgctgg
     5881 tcagaacgaa gtggctaccc gcttcaatac catgaccaaa aaagctgacg aaattcagat
     5941 ctacaaatat gtcgtgcata acgtagcgca ccgcttcggt aaaaccgcga cctttatgcc
     6001 aaaaccgatg ttcggtgata acggctccgg tatgcactgc cacatgtctc tgtctaaaaa
     6061 cggcgttaac ctgttcgcag gcgacaaata cgcaggtctg tctgagcagg cgctgtacta
     6121 cattggcggc gtaatcaaac acgctaaagc gattaacgcc ctggcaaacc cgaccaccaa
     6181 ctcttataag cgtctggtcc cgggctacga agcaccggta atgctggctt actctgcgcg
     6241 taaccgttct gcgtctatcc gtattccggt ggtttcttct ccgaaagcac gtcgtatcga
     6301 agtacgtttc ccagatccgg cagctaaccc gtacctgtgc tttgctgccc tgctgatggc
     6361 cggtcttgat ggtatcaaga acaagatcca tccgggcgaa gccatggaca aaaacctgta
     6421 tgacctgccg ccagaagaag cgaaagagat cccacaggtt gcaggctctc tggaagaagc
     6481 actgaacgaa ctggatctgg accgcgagtt cctgaaagcc ggtggcgtgt tcacggatga
     6541 agcgatcgat gcgtacatcg ctctgcgtcg cgaagaagat gaccgcgtgc gtatgactcc
     6601 gcatccggta gagtttgagc tgtactacag cgtctaatag ttgaagttgt actacccggc
     6661 gcacaacgcc gggatttagt tgccgtggaa actttcagcc catctctgca tgggcttttt
     6721 tctccgtcaa ttctctgatg cttcgcgctt tttatccgta aaaagctata atgcactaaa
     6781 atggtgcaac ctgttcagga gactgcttta tggcaacagg cacgcagccc gatgctgggc
     6841 agatcctcaa ctcgctgatt aacagtattt tgttaattga tgacaatctg gcgatccatt
     6901 acgccaaccc cgccgcgcaa caactgctcg cccaaagctc ccgcaaattg tttggtacgc
     6961 cgttaccgga actgttgagc tacttctcat taaatatcga gctgatgcaa gaaagtctgg
     7021 aggcggggca aggctttacc gataacgaag tgacgctggt catcgatggt cgctcgcata
     7081 tcctttctgt gacggcccag cgtatgccgg acggcatgat cctgctggag atggcaccga
     7141 tggataacca gcgccgctta agccaggaac aactacaaca tgctcaacaa gtagctgccc
     7201 gtgatttagt gcgcggcctg gcacatgaga ttaaaaatcc gcttggcggt ttacgtggcg
     7261 cggcgcagtt actcagcaaa gcgttacctg acccatcact actcgaatat accaaagtga
     7321 ttatcgaaca ggcggaccgg ctgcgaaatt tggtcgaccg tctgttgggg ccgcagctgc
     7381 ccggtacgcg cgttaccgaa agtattcaca aagtggctga acgcgtggta acgctggtgt
     7441 cgatggaact gccggacaac gtgcggttga ttcgtgatta cgatcccagc ctgccggaac
     7501 tggcgcacga cccggatcaa attgaacagg tcttgctgaa tattgtgcgc aatgcgctac
     7561 aggcgctggg gccggaaggc ggtgaaatca ttctgcgtac ccgcaccgcg tttcaactga
     7621 ccttacacgg cgagcgctac cggctggcgg cgcggattga tgtggaagat aacgggccgg
     7681 gcattccgcc tcatttgcag gatacgctgt tttacccgat ggtcagcggc cgcgaaggtg
     7741 gcaccgggct tggcttatcc atcgcccgta atttgattga tcagcattca ggcaaaattg
     7801 aatttaccag ttggccaggt cataccgagt tctcggttta cctgcctatc aggaaataaa
     7861 ggtgacgttt atgcaacgag ggatagtctg ggtagtcgat gacgatagtt ccatccgttg
     7921 ggtgcttgaa cgtgcgctcg ctggagcggg tttaacctgt acgacatttg agaacggcgc
     7981 ggaagtactg gaggcgctgg cgagcaaaac gccggatgtg ctgctttcag atatccgtat
     8041 gccgggaatg gacgggctgg cgctgctcaa gcagattaaa cagcgccatc caatgcttcc
     8101 ggtcatcatt atgaccgcac attccgatct ggatgctgcc gtcagcgcct atcaacaagg
     8161 ggcgtttgat tatctgccca aaccgtttga tatcgacgaa gcagtggcgc tggttgagcg
     8221 cgctatcagt cattaccagg aacagcagca gccgcgtaat attcagctta acggcccaac
     8281 gaccgatatc atcggcgaag cgcaagccat gcaggacgtg ttccgtatta tcggtcggct
     8341 ttcgcgttct tctattagcg tgctgattaa cggcgaatcc ggcaccggta aagaactggt
     8401 cgctcatgcc ctgcatcgcc acagtccgcg caccaaagcg ccgtttatcg cgctgaatat
     8461 ggcagctatc ccaaaagatt tgatcgaatc agaactgttt ggccacgaga aaggcgcgtt
     8521 tactggcgcg aataccattc gtcaggggcg ttttgaacag gccgatggcg gcacgttatt
     8581 tctcgacgaa attggtgata tgccgctgga tgtgcagacg cgtttgctgc gcgtgctggc
     8641 agacggtcag ttttatcgcg ttggcggcta tgcgccggtg aaagtggatg tgcggattat
     8701 cgctgccact caccagaatc ttgaacagcg ggtgcaggaa ggtaagttcc gtgaggatct
     8761 gttccaccgc ctgaacgtta tccgcgttca tctgccgccg ctgcgcgaac gtcgggaaga
     8821 tattccccgt ctggcgcgcc attttttaca ggttgccgcg cgcgaactgg gcgtagaagc
     8881 gaagttgctg catccggaaa ccgaagctgc tctgacgcgt ctggcgtggc caggcaacgt
     8941 gcgccagctg gaaaacacct gccgctggct aacggtgatg gccgccgggc aggaagtgtt
     9001 gattcaggat ttgcccggcg aactgtttga atcaacggtt gcggagagta cttcgcaaat
     9061 gcaaccggac agttgggcga cacttttagc gcagtgggca gacagagcgc tgcgttccgg
     9121 tcatcaaaat ctgctttccg aagcgcagcc agagctggag cggacgttac tgacgaccgc
     9181 gttgcgacat acgcaggggc ataaacagga agcggcgcgg ctactcggct ggggccgcaa
     9241 cactctgacg cgtaagttaa aagagctggg gatggagtga ttcacggctt gtgtgtaaag
     9301 attgattatt gagcgcaaat tgctggtatt ttgcgcttta ctgttccgat aagttcagta
     9361 tgatcttgcc cggaaaacgg ggagagtcat tatgctggaa tcaataatta atctggtatc
     9421 gagtggcgca gttgacagcc acacaccgca aactgccgtt gctgccgtgc tgtgtgccgc
     9481 gatgattggg ctgtttagct gaagctcgtt gtttatgccg gatgcggaga aagtcgtagg
     9541 cctgataaga cgcgaccagc gtcgcatcag gcatcatgct cagatgcctg atgcggcgta
     9601 aacgccttat ccggcctaca aaagcaataa tttcaacagg ttttgttgcc cagcgcatca
     9661 ggcaatcttg ttctcagcac attgatggcg ctttcgtttt ctactttgta aacgaagcgc
     9721 cattcactct ttaaatcacc cgtgagaact gctgcatccg cgctttctgg cgcagatagg
     9781 tatcaaagca catgcaaatg ttgcggatca gcaagcgacc tttcgccgtc acctgtattc
     9841 ccttctcatc cacatccacc agcccatctt ttgctaacgg ggcgagcagc ttgagatctt
     9901 ccgcaaagta atcagcgaag tgcaaatccc actgtttctc aagaggggcg taatccagac
     9961 ggaagttgca gatgagcgac ttaatcacat cgcggcgaat acagtcatca cgcgttagcg
    10021 caataccacg ccacagcgca ttgccttgtt catacacttg ctgatagtac tgcttcaact
    10081 ctttctggtt ctgcgcgtag cagtcgccaa tcatgctgat ggcggaaacg cccatcccca
    10141 gcagatcggt atcgccctga gtggtgtagc cctggaagtt acgatgcagc acgccttcac
    10201 gctgggccac cgccagctcg tcatcaggac gggcaaagtg atccataccg ataaactgat
    10261 agcccgattg cgtcaggaag gcgatggttt cctgcaggat atcgagtttt tgctgcggac
    10321 tcggcaggtc agcatctttg attttgcgct gagcagcaaa aatggtcggc agatgcgcgt
    10381 agttaaaaac gctcagacga tcggggttca gctccgccac acgtttcagg gtaaaggcga
    10441 aactctccgg cgtctgtttc ggcaggccgt aaatcaggtc gatgttggtg gaggtaaaac
    10501 caatctcacg cgcatggtta agcagtgcaa agatgaactc ttcatcctgc tcgcggttaa
    10561 ccagacgttg cacttctttg ttgaagtcct gcacgcccat gctcaggcga ttaaagcctt
    10621 cggcgcgtaa atgatcgagt acatccagtt cgatttcccg cggatcgact tcgatcgaaa
    10681 tctccgcatc ggtattgaaa tggaagtttt cgcgcagcag cttcatcaga cggctgattt
    10741 gcgctttatt cagatacgtc ggcgttccgc cgccccagtg caattggctg acgtgacgcc
    10801 cggcgaacag cggtgcacga tggacgattt cttgctccag cgcgtccaga tactgatcgg
    10861 ccttgtgctg ctggcgagta acaatcttat tgcaaccgca gaagtaacaa agcttatggc
    10921 agaacgggat atgtacgtag agagataatg gacgctcagg atagcgcgcc acggcttgta
    10981 aaaacgcctg ttcgccgaag tcttctgaaa actccagcgc ggtcgggtac gaggtgtatc
    11041 gtggcccgga atagttatat ttctggatca gggccagatc ccagtcgatt tgctgtacag
    11101 acatgctcac tccttccgat ggcgtctctg gcggctacgg cgtaccggcg gatgcccgtt
    11161 tcgggtcatc agccggttgc gcagccactt ttgtcgccgc gacaaccgct gtagtttaac
    11221 gaataaccac accagataac atataaccgg aagggttatc agcagaacgg gaaggcccac
    11281 ggcggcaatc cgttagttgc cccgcagcag acgcatcatg tcttcttgct tctcgtcttc
    11341 ttcctcttct tcgtcatcgt cataagagag gccgagtttc tgcatcaact catcaatacg
    11401 atccagttta gcatccaccc aggattgctc ttcggcgctc agggtttcgc ctgcttccag
    11461 acgttccagc agcgcatcca gacgctcatc cgtttccagt aattccaact ccgcctgcgg
    11521 tgaaagcata ggtttctcac tcttcggttt gtgctgtttg gtgacttttt cagtcactcc
    11581 caatggaata ggggttttac tgccaatacg tggatctttt ggtgcgttct ggcctttgct
    11641 gcctgacgtg gtgtttccgc ccgctgcgcg gctgcccggc gcatgaccac gacgtttttt
    11701 ctgacgctta cggtcacgag cttcctgatc cagctcctcg cgtgtttttc gacgcgcttt
    11761 tgcatgacct ttgctgcgtg agtttgaaga tgatggtttc ataatggttc gtcttttagc
    11821 cgttttattc agtatagatt tgcggcggaa tctaacagaa agcaagcaaa gaaaaaaggc
    11881 gacagattac tctgtcgcct tttttcctga ctcataaccc ttaacgggtc ttacaatcct
    11941 tgcaggccgc caacacaccc tgtttttcca ttagccggaa caatccgtgt tgattccatt
    12001 tccgtttaat tacgtctccg gacgtttgtc ttcctgacaa tcctctgtct tcgcctcctg
    12061 gcgctcctga ccctcatcct gagtcattgt tcctgttagc gtcctcgctc tatgggtgct
    12121 actttacgcc tttgctttaa acaaacaatc aaccgccatc agtatttgca cattttcaga
    12181 gttttatata aaagcaattc actgatttat atagcttatt tgacatgcat cgcagaattt
    12241 tctctgctat ttcgcataaa gactatcggc aatgtctcac aaagcgtatg gcttttactt
    12301 acaacgctca cgccgaaaaa tccgcctttt attatggttc aggtataatc gccacaaagc
    12361 tttgactgac ggagacgacc gctttgacta atttgaatta tcaacagacg cattttgtga
    12421 tgagtgcgcc tgatattcgc cacctacctt ccgataccgg aattgaagtg gcttttgcag
    12481 gccgttccaa cgcaggtaaa tccagcgcgc tgaacacgct gactaaccag aaaagcctgg
    12541 ctcgtacctc aaaaacccca gggcgcaccc agcttatcaa cctgtttgaa gtggctgacg
    12601 gcaagcgtct ggttgacttg cctgggtacg gttatgcgga agtcccggaa gagatgaagc
    12661 gcaaatggca gcgtgcgctc ggcgaatacc tcgaaaaacg tcagagcctg caaggtctgg
    12721 tggtgctaat ggatattcgc catccgctga aagatttgga tcagcagatg attgagtggg
    12781 cggtagacag caatatcgcc gttctggtgc tgctgaccaa agcggacaaa ctggcaagcg
    12841 gcgcacgtaa agcgctattg aatatggtgc gtgaagctgt actggcgttt aacggtgatg
    12901 tgcaggttga aacgttttct tcgttgaaga aacaaggcgt agacaagctg aggcagaaac
    12961 tggatacctg gtttagcgag atgcagcctg tagaagaaac gcaggacggc gaataatttt
    13021 cttgccttaa tgcttgtgcc ggatgtggcg tatccggccc gtaaattcaa tagattacaa
    13081 ttctaccgtt ggcctgataa acatagcgca tcaggcatta cggatctttt ctttcgccca
    13141 ataaaaaacg ccccagtcat tactgactgg ggcggctaaa atattcagcc aaatccgatt
    13201 acgtgaagta aaaggtctga aagatagaac atcttacctc tgtaccctac gcgactaact
    13261 ttactctttt ttgttcagtt cagaaagcac tttttgtaat tttttttcag ttgttgcata
    13321 ggaatttcta atagccatca caaaacgtga cagcttatgt tgcttactta cgaaaaaagg
    13381 gcatgttcag gcgaatctta gtgcgcctga tcccagtttt cgccactccc cacttccacc
    13441 agcaacggca catccagacg ggtacagttt tccatcagtt gatgaatctg cttcgcgacg
    13501 gcatcaacat catctttatg aacttcaaat accagttcat cgtgtacctg catgatcata
    13561 cgtacacgcg gttgctcagc ctgtaaccac gcatcaacgg caatcatcgc ccgtttgata
    13621 atgtcggcgg cggttccctg cattggcgcg ttaatggctg cacgttcagc cgctgcacga
    13681 cgagcaccat tgctggattt gatatccggc agatacagac ggcgtccgtc cagcgtttca
    13741 acgtagccca gctctttcgc ctgagcacgg gtgcgttcca tatactccag cacgccaggg
    13801 tagcgttcga agtaaaggtc catgtacttc tgcgcttctt tacgtggaat gttcaattgc
    13861 cgcgccagac cgaaagcact catgccataa atcagaccaa agttgatcgc tttcgcgcta
    13921 cggcgttgct cgctggtgac ggtttccagt ggcaaaccaa acacttctgc cgccgttgcc
    13981 cggtggatat cttttccttc cgcgaatgcg gtcagcaagc ctttatcacg cgagagatgc
    14041 gccataatgc gcagttcaat ctgcgagtag tccgctgaga caatcacata atcctctggc
    14101 gcaataaacg cctggcggat acgacgacct tcttcgttac gcaccggaat gttttgcagg
    14161 ttaggatcgg ttgacgataa acgtcccgtt gcagttactg cctggtgata agaggtatgc
    14221 acacgcccgg ttttcgggtt gatcatcagc ggcagcttgt cggtgtaagt cgacttcagc
    14281 ttcgccagac cacgatactc cagaatcact tttggcaacg gatagtccag cgccagttct
    14341 tccagtacct cttccgacgt tgacggcgcg ccacccggcg ttttcttcag cggtttaata
    14401 ccctgttttt caaacagaat ggtttgcaac tgcttggtgg aagaaaggtt aaattcttca
    14461 cctgcaattt catgcgcttt cttttccagc tcagccagac gcaaggtgag ctcttcagaa
    14521 tgattgtgca gcactttcgg atcgatcttc acaccgttac gttcaatgcg tgaaagcacc
    14581 ggcaccagcg gcatttcgat attctcgaag acgttcaacg gccctttgtg tttctgcaga
    14641 tccggccaca ttttcagatg caactgcaag gtgacatctg catcttcggc ggcgtaacgt
    14701 ccggcctctt cgagggcaat ctggttaaag gtcagttgat ttttgccttt acccgcaatc
    14761 tcttcaaaag tgatggtttt gtgcttcaac caacgttccg cgaggctgtc catatcgtga
    14821 cgcccggcaa cgctattgag aatgtaggac tccagcatgg tatcaaacgc aatcccacgc
    14881 agttcaatgc cgtaattcgc cagaataccg cgatcgtatt tcaggttttg cccgaccttc
    14941 agcgcctttt catcttccag cagcggtttt agcaactcga gtgcacgctc gtgagagatt
    15001 tgatcgggcg catcaagata atcatgagca accggaatat atgccgctac gcctggctcg
    15061 atagcaaaag aaagcccgac caggttagca gagatgttat caaggctgtc ggtttcggta
    15121 tcaaatgcaa ataccggcgc tttttccagc ttcgcaatcc acgctttcag tgtttcttca
    15181 tcaaggatag tgacgtagtt gtcataagaa atcaccgttg ccgtcacttc tggcgcttcg
    15241 tctgcaacac tggtttcctg cggcttcgcg gctgtttttg cccctttggc ctgtaaccat
    15301 ttgcccactt cgacatcagc agtccagcgt ttgaactcat actttttgaa cagccccaac
    15361 aactcttccg ctgccggttg ctgcacttcc agttgttcac aggtcagctc cagttcaaca
    15421 tcggttttaa tcgtcgccaa ctggtatgag agataagcaa cttctttgtt ttgctcgagc
    15481 ttcgctgcca ttgttttcgc gccacggaag ctcaacccag caattttttc tggctcggca
    15541 tacagcgtat ccagcccgcc aagaccttgc agcaatgcct gcgcggtttt ttcaccgacg
    15601 cccggtacgc caggaatgtt atcagaggag tcacccatca gcgccaggaa gtcgatgatc
    15661 aattctggcg gcacgccgta cttattcacc acctcttccg gtccgaggat ggtattcgtc
    15721 atggtattga taagcgtaat atttggcgtc accagctgcg ccatatcttt atcgccagtg
    15781 ctaatcagca ccggacgccc ggctttttcg gcttcgcgcg ccagagtacc gataacgtcg
    15841 tccgcttcta cgccagaaac cgccagcagc ggcagtccca tcgctttaac catcgcgtgc
    15901 agaggttcga tttgtgctcg cagatcgtcc ggcattggcg ggcgatgtga tttgtaatgt
    15961 tcaaacagtt catcacgaaa ggtttttccc ttggcgtcaa agaccaccgc tgcatgcgtc
    16021 ggtttatatt gcatgatcag actgcgcagc atgttgagga caccatacat cgcaccggtc
    16081 ggctcgcctg cgctgttagt cagcggggga aacgcgtgat atgcgcgata aagataagat
    16141 gaaccatcta caaggataag tggattttgg gggatctgaa ccataatgtc cgtgcctgtt
    16201 tatcagatta tgggtaaagg atgccacaga caggatgaaa acatgagttt ttcaccgctt
    16261 ttcgtgaaaa tcttttgtag atctgctgga tcgctcgcaa atcgtcatgt ggataacttt
    16321 gtgcataaat tatttacatc gcttttaatt tattatcatg ctttttacag catgatgttt
    16381 gtctttgttt ttcatgcagt taaaaataaa tccatcccta ataattgaga taatgggatt
    16441 ttttctaatg tggatagata tgaattattt ttctccttaa ggatcatccg ttatttgggt
    16501 cattttctgc taaaatctgc gccttgcgct tctttagtgc actgtctatg gctaaccttt
    16561 tgaataaatt cattatgacg agaatactcg ctgcgataac ccttttgctg agtatcgtat
    16621 tgaccatttt ggtcactatt ttttgttccg tcccgatcat cattgccggg attgtaaaac
    16681 tattgctacc tgtgccagtc atttggcgaa aggtttcgcg tttttgtgat tttatgatgt
    16741 attgctggtg tgaaggtctg gcggtattac tgcatctgaa cccacacctg caatgggaag
    16801 ttcacgggct ggaagggtta agtaagaaga actggtatct gcttatttgt aaccaccgta
    16861 gttgggcaga tattgtcgta ctgtgcgtgc tgtttcgtaa gcacattccg atgaataaat
    16921 atttcctcaa gcaacagctg gcctgggtgc catttcttgg cctggcgtgc tgggcgctgg
    16981 atatgccgtt tatgaagcgt tattcccgcg cttatttatt acgtcatccg gagcgacgtg
    17041 gtcaagatgt tgaaaccact cggcgttctt gtgaaaagtt tcgcctgcat cccaccacca
    17101 ttgtcaattt cgttgaaggc tcccgtttca cgcaagaaaa acatcagcaa acccactcca
    17161 cttttcaaaa cttgttgcca ccaaaggcgg caggcattgc gatggcgcta aatgtactgg
    17221 gtaaacaatt cgataaactg cttaatgtta cgctgtgtta tccggacaat aaccgtcagc
    17281 cgttcttcga tatgttaagc ggtaaactga cgcggattgt cgtacatgtg gatttacagc
    17341 ctattgccga tgagttacat ggcgactaca tcaatgacaa aagctttaag cgccattttc
    17401 agcaatggct gaactcattg tggcaggaaa aagaccggct tctgacgtct ctcatgtcat
    17461 cacagcgtca gaataagtaa gttttactcg cctctgaatg agcagaggcg agtgagtatt
    17521 ttaatgaaca aaacgtccgg cacgagacat aaattcttct tcgctcatct cctgtccgtt
    17581 aaaaacaact ttacccggtg tataacgcag ctgcagcgag gcggtattgt tgtcaatcgt
    17641 gattaactga aacatctgac ctaatgtcat catcccgctg atttgtttat cagcctgctt
    17701 ttgcgctttt tccgcatcca ttccttcaga taaattaagc tgttttgcgg tttctgtgac
    17761 gacattaagc ggtaacttca catcaaaatt gagcgatttg atatctttgt ttgtggatga
    17821 tgaagatttg gctgggtcgg caatactgat atccagattg gcatttagtt cgccgagtgc
    17881 gttcttccat cttaccggtt gtttaatggt cggctcactt ttttgtaata acggtaaata
    17941 ctctttgaac aatgcagcat tcacttcttg cagagcaact tcatcgtttg ctaactcagg
    18001 gtgtgcagca agctgcttct gcatcgcaat gttatactgg atgataaatt ggcgcactgc
    18061 tgagggatca atagattcag caatcaatga ccactcacca ctaccgagtt gctgattccc
    18121 ttttgttaac ttatcaagtt cgtaggtcag attgacattg atataatctt tgtctttaac
    18181 gcgatccagc ctggtttttg caacgaaagc atcgatttgg gcaacgtctt ccccgtgatt
    18241 gataatgtta atctgattaa atttcgactc gctttcacca accggaaatt tcttttcttc
    18301 taatcgcgcc agagaatcaa tggtaaaact ttttgccgtt actttgttca ttggtgataa
    18361 ttgcagaaca atatcttcga ctttgccttc aatatccagg ttagacaatg aactaccatc
    18421 accattaaaa gtaaactggc cctcaccaaa agaaaatttt ccatactctg caggggcaac
    18481 atctaatgtc gtagagaatg agccaccaaa cgcgaatcgc aatgtcgctt ccacgggcga
    18541 tttatttttc gcggcgataa atagcggttg tgtcagttca ttattcacta agttcacttt
    18601 tgccgctgcc agtgctggga tgacattacc atgcattaac attgtgatgg gcaacggacc
    18661 gtgctcaaca tccacatcaa atacaacttt ctggcctggc ttgatattga gatcgggtgc
    18721 accgttatcg aatgtcattt gcatttgaaa acgggaatta aaaaagcctt tgtcaaagtt
    18781 tttataactt aacgtcatat cgtaggcatg ttcacctttc tttttcgcat cgttaaaatc
    18841 tttaataaac ttttcgatac caggctgaat ttgcgtacct gtgtaccatg tgccaccacc
    18901 ccagattacg gctaacgcaa caataacacc tgtagctgac ttacgtatca tataaatatt
    18961 ccctgataaa acagcaaaac tttgaatatc cacttatgct gaaataatgt ccagcggcag
    19021 gatgcattat cagaatgtat ttatatttat taaataataa aaaaagcccg tgaatattca
    19081 cgggctttat gtaatttaca ttgaattatt tttcctcgga cagatatttc accgtatcag
    19141 catactgctg aacaaaaaca tccatattgc tggtatccat accctgcgga ttcagctgat
    19201 atttaccgtt aacaaacatc gccggaacac cacgcaattg cacatcagct gcggcttttt
    19261 cctgctgagc gaccagagat ttcaccacga agctgttcca cgccgcgtcg tactcttcac
    19321 ctttaatacc tgcattgata aatacatcgc ggatatcaga tgctgaacga atggtctggg
    19381 ttttctgtac gccttcaaac agcggaacag tcactttgtc ttccacgccc agcgccatcg
    19441 ccacagccca tgcctgagtc agatctttgc ccaggtcacc acccatgaag ttgacgtggt
    19501 atttagtcat cttcacgcct tccggcagtt ttttcttcac attatcagaa atatgcagaa
    19561 cttcttcaaa ctgatagcag tgcgggcaga agaaagagaa aaactccagc acttgcggcg
    19621 cgccagctac cggtttttcc agggtagtgt actgtttacc atcttcatac tgcgccgccg
    19681 atgcgctaaa cgctaaaact aaaccagcca gcgccagcca aatctttttc atgatctact
    19741 ctctccgatt aatacatagg tgttaattgc aaagggggtt cttgtagaac ttttgcctgt
    19801 tctataaaag tcgccgtctg tcgtagccag taatcttccc cggttaacca cgggaaattt
    19861 ttcgggaacg cgggatcagc ccaatgccgc attagccagg caagataata aaccaaacgc
    19921 atggcgcgta aaggttcaat cagtccgatt tcagcggtgt cgaactcgct aaattcttca
    19981 taagcttcaa taatagtttc cagttgcatc cgctgctggg ctttatcgcc attgagcaac
    20041 atccacaaat cctgaatagc tggaccatta cgtgcatcat ccagatcaac aaacattgga
    20101 ccatcgcgcc agagaatatt cccggcgtgg cagtctccat gtagccgcag aacggtgaaa
    20161 tcttcccgcc agtgtgctgt aacggcggcg atcagctcat ctgtcgcttt cagaaatgcc
    20221 gctttcaacc cggaaggtat cagtgtagcg tcctcaaaca gcttgcgagg ctcaatgaga
    20281 tattcattca gaccgatggt cgggcgatga ataaaaagct gtttgcgccc cgtctggtgc
    20341 atacgcccta aataacgccc aaccgcttcc atctgatcga tattatcagc ttcgaactgg
    20401 cgaccaccga cgcttggaaa aacagcgaaa taaaatccct gatgattcaa taaagtctga
    20461 ccgttaaagg ccacaggtgc tgcgaccgga acttcatcat ttaccagctg caacgcaaat
    20521 tgatgttctt cgaggatttg atcggctgtc caacgttcag ggcgataaaa tttgacgaca
    20581 aaacgtcgac gctcttcgtc ctgaaattga tagacacggt tttcatagct gttaagcggg
    20641 gtaagaccgg aatccacccg gatcccttgc ttaaacagag cgtccatgat ggtatccggg
    20701 tgtagtgtct ggaaagtaaa agcgctgttg ttcatcccat catccggaaa acattacgaa
    20761 tgatgaagga tatcatttca cgatgatttt ggtggcgccg cttacaagct tttactcttt
    20821 aattacgccg cgagcgcgta acagcgccgt tttgaagtct tcttcataat ctttctgcaa
    20881 acccgggatc accgcatctt tcgcggaatc acgcattttc aggtgataga tcagaatgtc
    20941 atccgtcaaa tccgccagtt cgccgtcaaa acctgactct ttcgccagtt tctgcaaaaa
    21001 ttgcaacaag ttaaagtctg gctctttttg ccaggctggc tggaggagtt caataacttc
    21061 attcagacgt ttacatttca tagtaatgct ccttactctt gagacagaca cgttagcagg
    21121 gtcaatccca caataaaaga ggcgatatcg gtgaatctaa tgacgacgat aacaggcgtt
    21181 gtgctggcag gcggtaaagc cagacgaatg ggcggcgtag ataaaggatt gctggactta
    21241 aatggcaaac cgctgtggca acatgtcgct gacgcgctta agacgcagct ctctcacgtc
    21301 gtggttaatg ctaatcgtca tcaggaaatc tatcaggcag gcggtctgaa agtgattgaa
    21361 gattcactgg cggattaccc aggccctctg gcaggaatgc tttcagtaat gcagcaggaa
    21421 gctggtgagt ggtttttgtt ttgtccgtgc gatacgcctt acattcccca tgatttagcc
    21481 gcccggctta atcatcagcg caaagatgcg cctgtcgtgt gggtccatga cggtgaacgc
    21541 gatcacccga ctattgctct ggtaaaccgc gctattgagc ctttattact ggaatatctg
    21601 caagcaggag aacgccgggt aatggcattt atgcgtctgg ctggcggtca tgcggttgat
    21661 ttcagcgatc ataaagatgc atttgttaac gtgaatacgc cagaggagct tgcccgatgg
    21721 caggaaaaac gatgataccg ttactcgcct ttgccgcgtg gagtggtacc ggaaaaacta
    21781 cgcttttgaa aaaactgatc ccggcattat gcgccagagg gatccgtccg gggctgatta
    21841 agcatacgca ccatgatatg gatgttgata agccaggcaa agatagctat gagctgcgca
    21901 aggctggcgc ggtacaaacc atcgttgcca gccagcaacg atgggccttg atgacggaaa
    21961 caccagacga agaagagctg gatctacatt ttctcgcaag tcgaatggat acctcaaagc
    22021 tggatttgat tctggtcgaa gggtttaagc atgaagagat cgcaaagatt gtgctgtttc
    22081 gcgatggagc cggacatcga cctgaagaat tagtgataga caggcatgtt attgctgtag
    22141 ccagtgatgt gccgcttaat cttgatgtcg cattactgga tattaatgat gttgaggggc
    22201 tggctgattt tgtggtggag tggatgcaaa agcagaacgg ataagcaaga gtaggctgga
    22261 tcagacgc
//
LOCUS       DAJTRD010000063        21530 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTRD010000063 DAJTRD010000000
VERSION     DAJTRD010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21530
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.063"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO315_002639"
     CDS             complement(19..1017)
                     /gene="fbp"
                     /locus_tag="OO315_002639"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5891497.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1193..2566
                     /gene="mpl"
                     /locus_tag="OO315_002640"
     CDS             1193..2566
                     /gene="mpl"
                     /locus_tag="OO315_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5891498.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2649..3200)
                     /locus_tag="OO315_002641"
     CDS             complement(2649..3200)
                     /locus_tag="OO315_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5891499.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3294..4646
                     /gene="pmbA"
                     /locus_tag="OO315_002642"
     CDS             3294..4646
                     /gene="pmbA"
                     /locus_tag="OO315_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5891500.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4829..5211
                     /gene="cybC"
                     /locus_tag="OO315_002643"
                     /pseudo
     CDS             4829..5211
                     /gene="cybC"
                     /locus_tag="OO315_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO315_002644"
     CDS             complement(5256..5720)
                     /gene="nrdG"
                     /locus_tag="OO315_002644"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5891501.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO315_002645"
     CDS             complement(5878..8016)
                     /gene="nrdD"
                     /locus_tag="OO315_002645"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5891502.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO315_002646"
     CDS             complement(8410..10065)
                     /gene="treC"
                     /locus_tag="OO315_002646"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5891503.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO315_002647"
     CDS             complement(10115..11536)
                     /gene="treB"
                     /locus_tag="OO315_002647"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5891504.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO315_002648"
     CDS             complement(11655..12602)
                     /gene="treR"
                     /locus_tag="OO315_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5891505.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12787..12840
                     /gene="mgtL"
                     /locus_tag="OO315_002649"
     CDS             12787..12840
                     /gene="mgtL"
                     /locus_tag="OO315_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5891506.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12981..15677
                     /gene="mgtA"
                     /locus_tag="OO315_002650"
     CDS             12981..15677
                     /gene="mgtA"
                     /locus_tag="OO315_002650"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5891507.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO315_002651"
     CDS             complement(15883..16269)
                     /gene="ridA"
                     /locus_tag="OO315_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5891508.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO315_002652"
     CDS             complement(16342..16803)
                     /gene="pyrI"
                     /locus_tag="OO315_002652"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5891509.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO315_002653"
     CDS             complement(16816..17751)
                     /gene="pyrB"
                     /locus_tag="OO315_002653"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5891510.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO315_002654"
     CDS             complement(17755..17889)
                     /gene="pyrL"
                     /locus_tag="OO315_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5891511.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18170..18565)
                     /locus_tag="OO315_002655"
     CDS             complement(18170..18565)
                     /locus_tag="OO315_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5891512.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO315_002656"
     CDS             complement(18697..19410)
                     /gene="bdcA"
                     /locus_tag="OO315_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5891513.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19481..20074
                     /locus_tag="OO315_002657"
     CDS             19481..20074
                     /locus_tag="OO315_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5891514.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20219..20671
                     /gene="tabA"
                     /locus_tag="OO315_002658"
     CDS             20219..20671
                     /gene="tabA"
                     /locus_tag="OO315_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5891515.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20794..>21530
                     /locus_tag="OO315_002659"
     CDS             20794..>21530
                     /locus_tag="OO315_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891516.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNDSQELNNSQKLNN
                     SQELNNSQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5202 a         5326 c         5673 g         5329 t
ORIGIN      
        1 gtggagaggc gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc
       61 aaccatatgg tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat
      121 atccagaata cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc
      181 catcgggttg cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg
      241 gtagagataa ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat
      301 ataacgcgag gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta
      361 cttcttcacc ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc
      421 tttctccggg aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta
      481 agtaaaggcg tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac
      541 cacgtaacct gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg
      601 cgtgccaaca ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac
      661 atcgatgttg gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca
      721 gccttcaaag acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg
      781 tgctttcagt gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc
      841 gccctgcacg ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat
      901 atcgcgatgg ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc
      961 ggtagcatga gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa
     1021 actttccctg caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa
     1081 tgttgcgctc aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac
     1141 ttgcccgaca tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat
     1201 tcatatttta ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt
     1261 aggccatgaa gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga
     1321 gaagcaaggc attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga
     1381 tctggtgatt attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga
     1441 aaaaaacatc ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg
     1501 ctgggtgctg gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg
     1561 gattctggaa cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt
     1621 tgaggtttcg gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga
     1681 ctgcgccttc ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct
     1741 caacaacctt gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca
     1801 gttccaccat ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga
     1861 catcaacctg aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga
     1921 gcagggtcac tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct
     1981 ggatggcgaa aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa
     2041 tggcctgatg gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa
     2101 cgcgctgggt tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg
     2161 cgtcacggta tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc
     2221 gctgcgtggc aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa
     2281 taccatgaaa atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga
     2341 agtcttcctg ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg
     2401 cgttcagcct gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac
     2461 cgctcagcct ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca
     2521 gaaactgctg gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct
     2581 gatatagcat tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac
     2641 aacgaaaatt acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg
     2701 gatttcggcg gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt
     2761 tgctgacgat cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga
     2821 tcgatcagac gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga
     2881 cggtttttca gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc
     2941 ttaccgatga gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca
     3001 atagccgggc gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca
     3061 acaatttccg cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg
     3121 acccagataa tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag
     3181 tcttcgggct gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa
     3241 gatgcgcact gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac
     3301 ttgcaatgaa agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt
     3361 ccactgcgct ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga
     3421 ccaccggcat tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg
     3481 atggcgcgct ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg
     3541 atttaagccc gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata
     3601 cctcgccaga tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga
     3661 atctcgactt gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc
     3721 gcgcagaaca ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct
     3781 ttaacagcca ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact
     3841 gctcaacgcg tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc
     3901 gtgattacgc ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg
     3961 gggccgactg tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga
     4021 aagcgccggt catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg
     4081 cgatagcggg tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac
     4141 aaattctgcc ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt
     4201 cgacgccatt cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca
     4261 tcctgactca gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg
     4321 gacatgcggg cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa
     4381 tgctcaaaga gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg
     4441 ccattaccgg tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc
     4501 agtatcccgt tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg
     4561 tcaccgtcgg taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc
     4621 cggagatgaa aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa
     4681 aaattacaca aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg
     4741 tactcgcagt acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat
     4801 gaagtgtaaa taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt
     4861 ctcctcgttg gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac
     4921 cctcaacgac aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc
     4981 gttaacgaag atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga
     5041 agataaatca ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt
     5101 cggtcagatt gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct
     5161 gcagagcaac tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt
     5221 tccctgttgc ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga
     5281 ttgctgctgc cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg
     5341 accagcacgt taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta
     5401 tagcctgtcc acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt
     5461 ttcagaatat ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc
     5521 tggcgtttga tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta
     5581 aatggctgac cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga
     5641 acacacccgg agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga
     5701 tagtactgat gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc
     5761 ggtaatcaat gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat
     5821 gtaggccgga taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta
     5881 acctatctgc ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc
     5941 gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac
     6001 acgggaggcg tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa
     6061 ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg
     6121 tacatgctga tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt
     6181 tggatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa
     6241 gtcgatcttg tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta
     6301 gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa
     6361 gcggtcgcac aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc
     6421 tttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag
     6481 cttctcgttg tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc
     6541 gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc
     6601 agcattcaga cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt
     6661 cacgccttcg agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc
     6721 atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag
     6781 gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt
     6841 ttcccacacg ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt
     6901 cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc
     6961 cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc
     7021 aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt
     7081 acgcaggatc gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa
     7141 ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga
     7201 ctggaaggca tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg
     7261 gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc
     7321 cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc
     7381 aataatctgc gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc
     7441 gttccccatt ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa
     7501 catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc
     7561 ctgcaccacg tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc
     7621 cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa
     7681 cgaggcgttg gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc
     7741 acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg
     7801 acccgacatc agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg
     7861 cccctgcatc tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac
     7921 ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac
     7981 tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca
     8041 ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct
     8101 ttccgcaatc gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta
     8161 gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag tctatatata
     8221 gtgctttgca tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc
     8281 gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa
     8341 atcaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt
     8401 agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca
     8461 gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg
     8521 ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc
     8581 cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt
     8641 gccccatgtc aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa
     8701 cacagaggag tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag
     8761 gccaatccac ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg
     8821 cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg
     8881 accatcgttg cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat
     8941 gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc
     9001 ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc
     9061 atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca
     9121 tgccacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc
     9181 aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt
     9241 aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct
     9301 ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat
     9361 ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc
     9421 caggtcttca gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc
     9481 gtcgacccca cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg
     9541 attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct
     9601 ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt
     9661 ttctggttcg ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc
     9721 ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg
     9781 aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt
     9841 gggatcaatc gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata
     9901 aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat
     9961 aacgccacgt aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat
    10021 ctggtagata acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca
    10081 aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc
    10141 ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca
    10201 ttgccagcgc aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc
    10261 ctacgccgat accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag
    10321 aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag
    10381 gctcagtgac cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt
    10441 gcttgcggct ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg
    10501 caatcagcgg ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa
    10561 gggtggtctg gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg
    10621 caccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc
    10681 caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg
    10741 aacagacggg caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt
    10801 caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca
    10861 ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat
    10921 aagcgttcat cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg
    10981 tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg
    11041 cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca
    11101 gcgtctgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac
    11161 cgccgctaat caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact
    11221 gctcatgcca tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg
    11281 cctgtccggt tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca
    11341 cctgaaattg cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt
    11401 tcggtctggc attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca
    11461 ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt
    11521 ttattttgct catcataaag ccccatggca gatgacattt ttggttggct gcagaatata
    11581 ttgcgcggaa aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat
    11641 cgttcaggaa acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc
    11701 ccggttacct gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga
    11761 tctacggtga cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc
    11821 agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg
    11881 tcggttgcgc acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag
    11941 ccttgcttca tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag
    12001 aacgccaggt aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg
    12061 agataactga tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc
    12121 ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc
    12181 gatgactgcc agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg
    12241 ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac
    12301 tgattttcca tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga
    12361 acggcgagat tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg
    12421 ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact
    12481 gcttcaacac gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa
    12541 accgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc
    12601 atcctgttgt cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca
    12661 gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata
    12721 aatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga
    12781 ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa
    12841 gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc
    12901 agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag
    12961 acagtgcgga gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac
    13021 cttcccgtct ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg
    13081 tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt
    13141 ggaaaacgtt cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg
    13201 aacaacatgg tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat
    13261 gggtctgcta tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg
    13321 ccacggaaga tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc
    13381 tgaactttat tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca
    13441 gtaataccgc gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga
    13501 tcccgataga ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc
    13561 cggcagattt acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg
    13621 gtgagtctct gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc
    13681 tggagtgcga caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa
    13741 tggtgattgc tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc
    13801 aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc
    13861 gctttatgct ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact
    13921 ggtgggaagc ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc
    13981 cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga
    14041 tcgtcaaaca tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata
    14101 aaaccggcac cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta
    14161 aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta
    14221 aaaatctgct tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg
    14281 ccagccgttg gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg
    14341 tggtagtggc agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa
    14401 tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca
    14461 tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg
    14521 cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg
    14581 acctgatcct cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg
    14641 cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt
    14701 tagttgcagc gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta
    14761 gtgatattga aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt
    14821 ttgcccgcct gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc
    14881 atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata
    14941 tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc
    15001 tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca
    15061 acatgctgaa atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc
    15121 tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga
    15181 acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa
    15241 ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac
    15301 cgatcagctc gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca
    15361 acacgccgga aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc
    15421 aaacgctgat tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat
    15481 cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt
    15541 caccgctggc cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg
    15601 ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc
    15661 gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa
    15721 agtgagcttt gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata
    15781 aattgcagga atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca
    15841 tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat
    15901 tgcttcgatc tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc
    15961 cgggaaagtg gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa
    16021 gtcgttcaga tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc
    16081 ggcttcgacg attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc
    16141 cggtacttca ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc
    16201 cagatcaacg ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt
    16261 tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca
    16321 gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc
    16381 acagtatttg catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga
    16441 aaccggttcg gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg
    16501 ctctggcaga cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt
    16561 ggcttgcggt gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc
    16621 gattttgatc agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat
    16681 gcgctgatct gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg
    16741 gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt
    16801 catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg
    16861 gcgagcgaaa atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac
    16921 atccgtcgca atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc
    16981 gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg
    17041 gtccagacgc tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc
    17101 ttcaatagag ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta
    17161 ttgcggcatt gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc
    17221 tagcgcctgg gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac
    17281 gtggagattg tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg
    17341 cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc
    17401 ggtggctagg cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt
    17461 gctgataacc gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc
    17521 gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag
    17581 gcgggtacgg gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc
    17641 tggttgcggg tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg
    17701 actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct
    17761 cctgacacct aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg
    17821 gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca
    17881 ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc
    17941 aagcatttga tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata
    18001 tatgcatttt tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca
    18061 gacatttcct gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt
    18121 catccgactt atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag
    18181 ggatacgcgc tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg
    18241 ccgtccagtt tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt
    18301 cagattgttt ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc
    18361 atcccgcagc tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa
    18421 aatcgggttc tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat
    18481 cgccggaacg aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag
    18541 ggaaaacagc ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag
    18601 atttatattt atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat
    18661 catggataat ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat
    18721 ggtatgcatc gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc
    18781 agcgacctct tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg
    18841 cattggcccg ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat
    18901 ggtgatccct cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga
    18961 tttactggcg gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc
    19021 gatgattaag atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata
    19081 aggagcatga atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc
    19141 atcgccaaag acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg
    19201 aacgacatca ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt
    19261 ctcttgtgcc agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc
    19321 cccatcggtg acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat
    19381 gagaactgtc ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc
    19441 attacaaaac tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc
    19501 tcgcgttcca ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt
    19561 gctttttcac caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg
    19621 tattaacccc ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt
    19681 actcaatgaa tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg
    19741 tccggtaggc gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa
    19801 cggcggctgc gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg
    19861 tgatattgcc attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag
    19921 gcggcatcca caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct
    19981 ttctgcgaag gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc
    20041 aggggaagcg ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga
    20101 acgcgcctgc cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg
    20161 atcatctttt cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat
    20221 gatcatcgga aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat
    20281 tgagcatatc aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg
    20341 caatcgtctg ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc
    20401 ggagtaccat gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac
    20461 cttcagcacg caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc
    20521 atttttgccg gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt
    20581 gttttatccg ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg
    20641 caaagcagta gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt
    20701 tacggcgagt ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg
    20761 cttagacgtt cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat
    20821 tctcaacaca ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg
    20881 tatgtgggaa tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt
    20941 ctttctccta gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat
    21001 tttagcaaac gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct
    21061 tattctaaag atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg
    21121 gagcgagcgg atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa
    21181 gggattcgtg aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt
    21241 tatgaaggga agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag
    21301 gttctggcgt gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat
    21361 cagaagttaa ataatataca ggagttaaat gattcgcagg agttaaatga ttcgcaggag
    21421 ttaaataatt cacagaagtt aaataattcg caggagttaa ataactcgca ggtgagttgt
    21481 aaaggttcag ttgattctac gattacggat ttattagaaa aagcattgaa
//
LOCUS       DAJTRD010000064        21498 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTRD010000064 DAJTRD010000000
VERSION     DAJTRD010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21498
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.064"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(37..2106)
                     /gene="glyS"
                     /locus_tag="OO315_002660"
     CDS             complement(37..2106)
                     /gene="glyS"
                     /locus_tag="OO315_002660"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5891517.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="OO315_002661"
     CDS             complement(2116..3027)
                     /gene="glyQ"
                     /locus_tag="OO315_002661"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5891518.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3122..3421)
                     /locus_tag="OO315_002662"
     CDS             complement(3122..3421)
                     /locus_tag="OO315_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5891519.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3596..4591
                     /gene="wecH"
                     /locus_tag="OO315_002663"
     CDS             3596..4591
                     /gene="wecH"
                     /locus_tag="OO315_002663"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5891520.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4633..5070)
                     /locus_tag="OO315_002664"
     CDS             complement(4633..5070)
                     /locus_tag="OO315_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5891521.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5116..5457)
                     /locus_tag="OO315_002665"
     CDS             complement(5116..5457)
                     /locus_tag="OO315_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891522.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="OO315_002666"
     CDS             complement(5626..7080)
                     /gene="xylB"
                     /locus_tag="OO315_002666"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5891523.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="OO315_002667"
                     /pseudo
     CDS             complement(7152..8474)
                     /gene="xylA"
                     /locus_tag="OO315_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8840..9832
                     /gene="xylF"
                     /locus_tag="OO315_002668"
     CDS             8840..9832
                     /gene="xylF"
                     /locus_tag="OO315_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5891524.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9910..11451
                     /gene="xylG"
                     /locus_tag="OO315_002669"
     CDS             9910..11451
                     /gene="xylG"
                     /locus_tag="OO315_002669"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5891525.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11429..12610
                     /gene="xylH"
                     /locus_tag="OO315_002670"
     CDS             11429..12610
                     /gene="xylH"
                     /locus_tag="OO315_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5891526.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12688..13218
                     /locus_tag="OO315_002671"
     CDS             12688..13218
                     /locus_tag="OO315_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5891527.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13330..13866
                     /locus_tag="OO315_002672"
     CDS             13330..13866
                     /locus_tag="OO315_002672"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5891528.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13974..14798)
                     /locus_tag="OO315_002673"
     CDS             complement(13974..14798)
                     /locus_tag="OO315_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5891529.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15118..17147
                     /gene="malS"
                     /locus_tag="OO315_002674"
                     /pseudo
     CDS             15118..17147
                     /gene="malS"
                     /locus_tag="OO315_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="OO315_002675"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="OO315_002675"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5891530.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="OO315_002676"
     CDS             complement(18729..19202)
                     /locus_tag="OO315_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5891531.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO315_002677"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO315_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5891532.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..21344
                     /gene="yiaK"
                     /locus_tag="OO315_002678"
     CDS             20352..21344
                     /gene="yiaK"
                     /locus_tag="OO315_002678"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5891533.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRILP
                     MGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPTRD
                     AKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21356..>21498
                     /locus_tag="OO315_002679"
     CDS             21356..>21498
                     /locus_tag="OO315_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5891534.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATNFNVLEPGVVEIDGKN
                     IYA"
BASE COUNT         5401 a         5489 c         5277 g         5331 t
ORIGIN      
        1 gcctgccaga tggcaggctt tttaataacg gcgttattac tgcaacagcg aaatatccgc
       61 aacgcgcagg aacagttcgc gcagtttctc cagcatggtc agacggttga gacgcaattc
      121 tttgtcatca accatgacca tcactttatc gaagaaagca tcaaccggct cacgcagctc
      181 tgccagttcg accagcgcat cctggtaacg accttccgca aagtacggct ccagcttgtc
      241 acgtagcacc acaacctgca tcgccagttt aatttcttcc ggctctttca gggtagaggc
      301 attcacgcga tcgctcagca cttcgtcaga tttcgccagg atgttagata cacgtttgtt
      361 cgccgccgcc agtgctgcag ctgcttccag ggtacggaag tgcgataccg ctttcatgcg
      421 ggcatcgaaa tcagccggac gagtcggacg acgcgccagt accgcctgga tggtgtcaac
      481 ggtataacct tcgtcctgat accaggcgcg gaagcgaccg agcataaagt cgataacatc
      541 atcaactacg ttggcattag tcagcttatc gccatacaga cgcaccgctt cttcggtcag
      601 cgtttgcaga tcaaggttga ggttcttctc aacgataatt cgcagcacgc caagtgcggc
      661 acgacgcagc gcaaacgggt ctttgtcgcc tttcggatgc tgaccgatac cgaagatacc
      721 cgccagagta tccatcttat cagcaatcgc cagcgcacaa gctaccgggt tggacggcag
      781 gtcatcaccg gcaaagcgcg gctgatactg ctcattcagc gcgacggcta cgtcttccgc
      841 ttcgccatcg tgacgcgcgt agtgcatccc cataacgcct tgggtgtcgg tgaactcgaa
      901 gaccatgttg gtcatcaggt cacacttaga cagcaggccc gcacgggttg cgtggttaac
      961 gtcagcgcca atctgttcgg cgatccagcc agccagcgcc tggatgcggt cagtcttgtc
     1021 acgcagcgta cccagctgtt gctggaacag cacggtttgc aggcgcggca ggttatcttc
     1081 cagacgtttt ttgcggtcgg tgttgaagaa gaactcggca tccgccagac gtggacgaac
     1141 gactttctcg ttaccagaga taatttgctg cggatctttc gattcgatgt tggcaacgaa
     1201 gataaagttc ggcagcagtt tgccgtcgtt cgcatacacc gggaagtact tctggtcacc
     1261 tttcatggtg taaaccaggg cttcagccgg caccgcgagg aatttctctt cgaattttgc
     1321 ggtcagaacg accggccact ccaccagcga agccacttct tccagcaggc tttcgcttaa
     1381 gtcagcgtta ccgccaatct tacgcgctgc ttcttcggca tcggctttaa tcttcgcttt
     1441 acgttcttcg taatcggcga tgactttccc gcgctcacgc agaatttccg gatactgatc
     1501 ggcgttgtcg atggtgaatt ccggttcgcc cataaagcgg tggccgcgaa tcacgcgatc
     1561 ggactgaatg cccagaatgg ttgccggaat gactttgtcg cccagcagca gggtcacggt
     1621 gtgcaccgga cgcacgaagt gcacgtcgct tgcaccccag cgcatcagtt tcgggatcgg
     1681 cagtttcgcc agcgaagtcg caaccatatt cggcagcagt gcttcggtgc tttcgccctt
     1741 cacatgggcg cgatacagca gccattcgcc tttatcggta gtcagacgct cagcctggtc
     1801 aacggtaata ccacaaccac gcgcccaacc ttctgccgct ttgctcggtt ttccttcagc
     1861 gtcgaacgcc tgggcaatcg ccgggccgcg tttttcgatt tcacgatctg gttgtgcttc
     1921 cgccaggtta gctactttca gcgccagacg acgcggagca gcaaaccatt gaacggtgcc
     1981 gtgtgcgagg cctgcgttat ccagctccgc agtaaagttc gcagcaaagg actctgccag
     2041 gctgcgcagt gcttttggtg gcagctcttc agtgccgatt tccaccagaa aagttttctc
     2101 agacatagcc gcctcttact tatctttgtt gcacatcggg aagccgaggg cttcacggga
     2161 agcgtaatat gcttctgcca ctgctttggt cagggtgcga atgcgcagaa tgtagcgctg
     2221 acgctcggtg acggagatgg ctttacgcgc atccagcagg ttgaagctgt gggcggcttt
     2281 cagaatacgc tcgtaggctg gcagcggcag cggattttcc agcgccagca gctgctgcgc
     2341 ttctttctcg tactgctcaa agcaggtgaa caggaagtcc acatccgcgt attcgaagtt
     2401 gtaagtggac tgctccactt cgttctgatg gaacacgtcg ccgtaggtgg ttttacccag
     2461 cgggccgtcg ctccagacta ggtcgtaaac gctgtctacg ccctgaatgt acatggccag
     2521 acgttccaga ccgtaggtga tctcgccggt aaccggttta cactccagac caccaacctg
     2581 ctggaagtaa gtgaactgcg tcacttccat gccgtttagc cacacttccc agcccagtcc
     2641 ccaggccccc agagtcgggt tttcccagtt atcttccacg aaacggatgt cgtgaatagt
     2701 cgggtccatg cccagctctt tcagagaacc gaggtacagc tcctgaatat tgtccggcga
     2761 tggcttaatg accacctgga actgatagta gtgctgtaaa cggttggggt tttcgccgta
     2821 gcgaccatcg gtcgggcgac gagaaggctg aacataagca gccgccatcg gttctggccc
     2881 cagcgcgcgc agacaggtca ttgggtgaga ggttcccgcg ccgacttcca tgtccaatgg
     2941 ttgaacaatg gtgcagccct ggcgagccca gtaatcctgt aaggtcagga tcaagccctg
     3001 gaaggtcctg gtatcaaact tttgcatatt atttcgtgct ggatacgtgt ggatttaaag
     3061 gaagggatca gtatacccgc tggatggaag atatacagta cgaaacggga aaaagcaggg
     3121 cttaacgcat ggaaagatgc aaaaaatggc catctgcgtc aaaagaacag acaaactgct
     3181 ctttacggaa ggtataaccg cgcatttcat agcttccctg gaactgttcg aaggcggtga
     3241 cgtcgatttt ttgctcgccg gtgttataac gttttgccgc ctgatccttg cataacgctt
     3301 ccatattcaa cgaacgcaga ggatcaaccc ttacccgttg tgctttctgc acgggtgacg
     3361 gtgtagaaca acccactagc accataagcg ccattgccgg aaagaaagcg ttcatcatca
     3421 ttaaattacc gtctggtttg caggtgagtc ttattattta tatcggtata gtggctgata
     3481 tcttacgaat ttccttaagc ctctataggc tgtagtgata aaactcagat tattccagtg
     3541 cgccaaccaa atgatttatc ggtcagactt tttttaacca gatacagaga cacagatgca
     3601 gcccaaaatt tactggattg ataacctgcg agggatagcg tgtttaatgg tggtgatgat
     3661 tcacaccact acctggtatg tgaccaatgc tcatagtgtt agccccgtca catgggatat
     3721 cgccaatgtt ctgaattctg cctctcgtgt cagcgtgccg ctatttttca tgatttccgg
     3781 ctatctcttt tttggcgaac gcagcgccca gccgcgccat ttcttgcgta tcggcttatg
     3841 tctgattttt tatagcgcaa tcgcactgct ctacattgcg ctgtttacct ccatcaatat
     3901 ggagttagcg ctgaaaaacc tgctgcaaaa gccagtgttt taccacttgt ggtttttctt
     3961 cgcgattgcg gtgatttatc tggtttcacc gctgattcag gtgaagaacg tcggcggaaa
     4021 aatgttgctg gtgctaatgg tggtgattgg cattatcgct aacccaaaca cagtgccgca
     4081 gaaaattgac ggttttgaat ggctgccaat taacttatat atcaatggcg atacttttta
     4141 ctacattctg tatggcatgt tgggccgcgc tatagggatg atggacacac agcataaagc
     4201 actgtcgtgg gtgagcgccg cgctgtttgc gacgggggtt tttattatct ctcgcgggac
     4261 attatatgaa ttgcagtggc gcggaaattt tgccgatacc tggtatcttt actgtgggcc
     4321 gatggttttt atctgcgcaa tcgcgctatt gactctggtt aaaaacacgc tggatacgcg
     4381 taccattcgc ggacttggct taatctcccg ccattcattg ggtatatacg gattccacgc
     4441 cttgattatc catgcgctgc gcacccgggg aattgagctt aaaaattggc caatactgga
     4501 tattatttgg atcttttgcg cgacgttggc agcgagtttg ttactttcta tgctggtaca
     4561 acgaatcgac agaaacagat tagtgagtta agtaaaagcc cgttcacatt ggactgaccg
     4621 ggcttacgtg aattattcgc tgtattcttc ctgggtaacc tgtgtttctt ttggcgggtt
     4681 tattccggca tcacgaatat tcttctgcac cgctactgca ccaaacaagc ttaagaagaa
     4741 agccagtcca taaaaacctt tttcactgag taataatgtc gcattccaca gacctaccat
     4801 cagtagagca acagagataa taaagacagt caggcaggtc atataataaa tggaagtggt
     4861 cggtatgcct tcatacttgt cccgaacggt cttttgataa gacgcagcag aaaacagtcc
     4921 taacaccagt acggcaaaat aatacccttt ttcatttaac tgcatctctg cattccatag
     4981 ccctaacagg taggtaacga taccaccaac gagagctatc catgacacaa tactaaaggc
     5041 cggtgaatag gttgatattt tgttgtccat cacagtattc ctttcattcc tgaatgtgta
     5101 agagctttat gttgctcata ccgagcgaaa caaacgcatt tgaccaaaca agcagacaaa
     5161 aaaggcgatg gggtagatca ccatttcata agcattgata ggtgcattta aaacaccgat
     5221 aaacaagaga ccgatagtga ttaacgcaac taactggcag atatgggtaa aaaaattatc
     5281 cagttgcccg agtttttctg cgcgaagata tgcatagtta ccaaaagttg ctgtcattaa
     5341 cacgccaaga aaatagcctt ttccacttag caatgggcat gagatccata gcccaaccag
     5401 ataaaccagc gccccgacaa caaataatag ttttgccaca gtttttgagg ttttcataac
     5461 gatctccata tctaccagcg atacattacg agtaaccaac gaaagacaaa actgaaaaat
     5521 gccattaaca aatgattttc agaataaatt catactaaat attaattaat tagtgagata
     5581 tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg
     5641 cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg
     5701 ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga
     5761 tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc
     5821 cccccccgta cggtaatcga gctgctgacc gctgatatcc gccagcatct gacgccagta
     5881 ctcactacgc gccccgccac caatcaacgt aacactttgc ggtttaatgc cgcaggcatg
     5941 cacgacatcc atgccatctg ccagcgcata acccacgcct tccagcactg ctcgcgccag
     6001 ttcattgggg ccatgttgat gagtcaaacc aaagaaaacc cccttcgcct ggggattatt
     6061 gtgtggtgta cgctcgccgg aaagataagg cagaaaccaa actggctcgg cactttcatc
     6121 agcctgttga gctgcagcga ttaaagctgg gacattgcac aggccggtta atttcgcggc
     6181 ccaatccaga cacgatgctg cactcagcat tacagacatt aaatgccaac gctgcggtag
     6241 cgcatggcaa aagctgtgta cagcgctttc tggcttgctt aagaaccctt cgctgacagc
     6301 aaaatagacc cccgacgtcc ccagcgataa cattgcctga ttagcatcaa ccattcccac
     6361 accaactgca ccagctgcat tgtcgccacc gcctgcgaca actggcaccg tcgccatacc
     6421 ccacgctttc gcaacttcag gtaacaaagc accagtaatt tcgctgcctt cgtataatgc
     6481 gggcatctgg tcacgagata agtcgcaagc ctgcagcatg acgtcactcc agtcacgctt
     6541 tgcgacatcc agccacatgg tgccagctgc gtcagacata tcgctggcaa actcccccgt
     6601 catacgcaga cgcaagtaat ctttcggtaa taatactttg tcgatttgac ggaatatctc
     6661 cggctcatgc cgctgaaccc atagcaattt aggcgcagta aatccgggca tcatcaggtt
     6721 gccggtaatc actcgtgatt gcggaactct cgcttccagc aaagtgcact cttgcgcaca
     6781 gcgcccgtcg ttccacaaaa tggcagggcg caatacccgt tgttgagcat ccagtaaggt
     6841 tgctccatgc atctggccgg caatacccaa tgctttaacg tcctgcagag aatgctgatc
     6901 gcccagagct ttcattgcgc gatcagttgc ctgccaccac tgttccgggt cttgttccga
     6961 ccaaagtgga tgcgggcgcg aaacggtcag cttttccgtt tgcgaagcaa ccacctcacc
     7021 ctgctcgttg agcaaaataa cttttacgcc cgaggtgcca agatctatcc cgatatacat
     7081 atcgatcgtt ccttaaaaaa atgcccggta tcgctaccga taaccgggcc aacggactgc
     7141 acagttagcc gttatttgtc gaacagataa tgatttacca gattttccag ttgctcctgg
     7201 cgaccactct gatgcaccgg agacaaatta tgttcctgag catatttggc taaatctgcc
     7261 agtgacattt ggcctttcag gatttgctgg cccaattcgc tattccagcc ggaataacgc
     7321 tgcgcgatgc gtttatccag ctcgccatct tcaatcatgc gcgctgcaat tttcagcgcc
     7381 agtgccatcg tatccatcgc gccgatatga ccgtaaaaca gatcatattt atcagtactt
     7441 tgacgacgta ctttggcatc gaagttcaga ccaccggtgg tgaaaccgcc tgctttgaga
     7501 atttcataca tcaccagcgc attctcttcc acactgttcg ggaactggtc ggtgtcccag
     7561 cccagttgcg catcgccacg gttggcgtcg acagaaccga acaggccaag cgcaatggcg
     7621 gtggctattt catgatggaa agagtgacct gccagcgtcg cgtggttagc ttcaatgttc
     7681 agtttaatct ctttttccag accaaactgt ttcaggaagc catagaccgt cgcggcatcg
     7741 taatcatatt gatgtttagt cggttcttgc ggtttcggtt cgataagcaa cgtgccctag
     7801 aagccgattt tatgtttatg ctcaaccacc atctgcataa agcggcccag ttgttcacgc
     7861 tcctgacgca agtcggtatt taacagcgtt tcgtaacctt cacgaccacc ccacaggaca
     7921 tagttttcac cgcccaattt atgggttgct tccatcgctg taacaacttg cgttgccgcc
     7981 cagctgaaga cttcaggatc tgggttcgtc gccgcacccg cgccatagcg agggtttgta
     8041 aagcagttag cggttcccca cagcagcttc acgccgctct cttcttgctt ggctgccagg
     8101 acatcaacca tttgcgcaaa attattgatg tactctttta acgacgcgcc ctcaggggaa
     8161 acatccacat cgtggaagca ataaaatggc acatgtaact tgtggaaaaa ctcaaatgcg
     8221 acatctgctt tacgcttcgc caacgccagt gcctcaccag gctgctgcca cggacgatta
     8281 aacgccccca caccaaacat gtccgccccg ttccagcaga aggtgtgcca gtagcaggcg
     8341 gcaaaacgca agtgctcttc catacgctta cccaacacca gttcgtcggg attgtagtga
     8401 cggaatgcta acgggtttga ggattttgag ccttcataac gaacgcgatc gagctggtca
     8461 aaataggctt gcatattgaa ctccataatc aggtaatgcc gcgggtgatg gatgatgtcg
     8521 taatattggg cactcccttt caattgctca attatgttat ttcacactgc tattgagata
     8581 attcacaagt gtgcgctcgc tcgcaaaata aaatgggatg atgaaaatgg gtaattcctc
     8641 gaagataaaa atgcaataag tacaattgcg caacaaaagt aagatctcgg tcataaatca
     8701 agaaataaac caaaaatcgt aatcgaaaga taaaaatctg taattgtttt cccctgttta
     8761 gttgctaaaa attggttacg tttatcgcgg tgattgttac ttattaaagc tgtcctctaa
     8821 ctacagaagg ccctacacca tgaaaataaa gaacattcta ctcacccttt gcacctcact
     8881 tctgcttacc aacgttgcgg cacacgccaa agaagtcaaa ataggtatgg cgattgatga
     8941 tctccgtctt gaacgctggc aaaaagatcg agatattttt gtgaaaaagg cagaatctct
     9001 cggtgcgaaa gtatttgtac agtctgcaaa tggcaatgaa gaaacacaaa tgtcgcagat
     9061 tgaaaacatg attaaccggg gtgtcgatgt tcttgtcatt attccgtata acggtcaggt
     9121 attaagtaac gttgtaaaaa aagccaaaca agaaggtatt aaagtattag cttacgaccg
     9181 tatgattaac gatgcggata tcgattttta tatttctttc gataacgaaa aagtcggtga
     9241 actgcaggca aaagccttgg tcgatattgt tccgcaaggt aattacttcc tgatgggcgg
     9301 ctcgccggta gataacaacg ccaagctgtt ccgcgccgga caaatgaaag tgttaaaacc
     9361 ttacgttgat tctggaaaaa ttaaagtcgt tggtgaccaa tgggttgatg gctggttacc
     9421 ggaaaacgca ttgaaaatta tggaaaacgc gctaaccgcc aataataaca aaattgatgc
     9481 tgtagttgcc tcaaacgatg ccaccgcagg tggggcaatt caggcattaa gcgcgcaagg
     9541 tttatcaggg aaagtagcaa tttccggcca ggatgcggat ctcgcaggta ttaaacgtat
     9601 tgctgccggt acgcaaacta tgacggtgta taaacctatt acattgctgg caaatactgc
     9661 cgcagaaatt gccgttgaat tgggcaatgg tcaggagcca aaagcagata cctcactgaa
     9721 taatggcctg aaagatgtcc cctcccgcct cctgacaccg atcgatgtga ataaaaacaa
     9781 catcaaagat acggtaatta aagacggatt ccacaaagag agcgagctgt aagcgttacg
     9841 ccccggcgcg gagcgggggc gtgatttctc tccatgccgc gtgaatgaat tggcttaggt
     9901 ggagtcgtta tgccttatct acttgaaatg aagaacatta ccaaaacctt cggcagtgtg
     9961 aaggcgattg ataacgtcag cttgcggttg aatgctggcg aaatcgtctc actctgtggg
    10021 gaaaatgggt ctggtaaatc aacgctgatg aaagtgctgt gtggtattta tccccatggc
    10081 tcctacgaag gcgaaattat ttttgcggga gaagagattc aggcgagtca catccgcgat
    10141 accgaacgca aaggtatcgc catcattcac caggaattgg ccctggtgaa agaattgacc
    10201 gtgctggaaa atatcttcct gggtaacgaa ataacccaca atggcattat ggattatgac
    10261 ctgatgacgc tacgctatca gaagctgctc gcacaggtca gtttatccat ttcacctgat
    10321 acccgcgttg gcgatttagg gcttgggcaa caacaactgg ttgaaattgc caaggcactt
    10381 aataaacagg tgcgcttgtt aattctcgat gaaccgacag cctcattaac tgagcaggaa
    10441 acgtcggttt tactggatat tattcgcgat ctacaacagc acggtatcgc ctgtatttat
    10501 atttcgcaca aactcaacga agtcaaagcg atttccgata cgatttgcgt tattcgcgac
    10561 ggacagcaca ttggtacgcg tgatgctgcc ggaatgagtg aagacgatat tatcaccatg
    10621 atggtcgagc gagagttaac cgcgatttac cctaatgaac cacataccac cggagatgaa
    10681 atattacgta ttgaacatct gacggcatgg catccggtca atcgtcatat taaacgagtt
    10741 aatgatgtct cgttttccct gaaacgtggc gaaatactgg gtattgccgg actcgttggt
    10801 gccggacgta ccgagaccat tcagtgcctg tttggcgtgt ggcccggaca atgggaagga
    10861 aaaatttata ttgatggcaa acaggtagat attcgtaact gtcagcaagc catcacccag
    10921 gggattgcga tggtacccga agacagaaag cgcgacggca tcgttccggt aatggcggtt
    10981 ggtaaaaata ttaccctcgc cgcactcaat aaatttaccg gtggcattag ccagcttgat
    11041 gacgcggcag agcaaaaatg tattctggaa tcaattcagc aactcaaagt taaaacgtcg
    11101 tcccccgacc ttgctattgg acgtttgagc ggcggcaatc agcaaaaagc gatcctcgcc
    11161 cgctgtctgt tacttaaccc gcgcatcctc attcttgatg aacctaccag gggtattgat
    11221 attggcgcga aatatgagat ctacaaatta attaaccaac tcgtccagca gggtattgcc
    11281 gttattgtca tctcttccga attacctgaa gtgctcggcc ttagcgatcg tgtactggtg
    11341 atgcatgaag ggaaactaaa agccaacctg ataaatcata acctgactca ggagcaggtg
    11401 atggaagccg cattgaggag cgaacatcat gtcgaaaagc aatccgtctg aagtgaaatt
    11461 ggccgtaccg acatccggtg gcttttctgg gctgaaatca ctgaatttgc aggtcttcgt
    11521 gatgattgca gctatcatcg caatcatgct gttctttacc tggaccaccg atggtgccta
    11581 cttaagcgcc cgtaacgtct ccaacctgtt acgccagacc gcgattaccg gcatcctcgc
    11641 ggtaggaatg gtgttcgtca taatttctgc tgaaatcgac ctttccgtcg gctcaatgat
    11701 ggggctatta ggtggcgtcg cggcgatttg tgacgtctgg ttaggctggc ctttgccact
    11761 taccatcatt gtgacgttgg ttctgggact gcttctcggt gcctggaacg gatggtgggt
    11821 cgcgtaccgc aaagtccctt catttattgt caccctcgcg ggcatgttgg catttcgcgg
    11881 catactcatt ggcatcacca acggcacgac tgtatccccc accagcgccg cgatgtcaca
    11941 aattgggcaa agctatctcc ccgccagtac cggcttcatc attggcgcgc ttggcttaat
    12001 ggcttttgtt ggttggcaat ggcgcggaag aatgcgccgt caggctttgg gtttacagtc
    12061 tccggcctct accgcagtag tcggtcgcca agctttaacc gctatcatcg tattaggcgc
    12121 aatctggctg ttgaatgatt accgtggcgt tcccactcct gttctgctgc tgacgttgct
    12181 gttactcggc ggaatgttta tggcaacgcg gacggcattt ggacgacgca tttatgccat
    12241 cggcggcaat ctggaagcag cacgtctctc cgggattaac gttgaacgca ccaaacttgc
    12301 cgtgttcgcg attaacggat taatggtagc catcgccgga ttaatcctta gttctcgact
    12361 tggcgctggt tcaccttctg cgggaaatat cgccgaactg gacgcaattg cagcatgcgt
    12421 gattggcggc accagcctgg ctggcggtgt gggaagcgtt gccggagcag taatgggggc
    12481 atttatcatg gcttcactgg ataacggcat gagtatgatg gatgtaccga ccttctggca
    12541 gtatatcgtt aaaggtgcga ttctgttgct ggcagtatgg atggactccg caaccaaacg
    12601 ccgttcttga ttttgataaa aattttctca aagccggtta cgtattaccg gttttgagtt
    12661 tttgcatgat tcagcaggaa aagaaccatg tttactaaac gtcaccgcat cacattactg
    12721 ttcaatgcca ataaagccta tgaccggcag gtagtagaag gcgtagggga atatttacag
    12781 gcatcacaat cagaatggga tattttcatt gaagaagatt tccgcgcccg cattgataaa
    12841 atcaaggact ggttaggaga tgtcgtcatt gccgacttcg acgacaaaca gatcgagcaa
    12901 gcgctggctg atgtcgacgt ccccattgtt ggggttggcg gctcgtatca ccttgcagaa
    12961 agttacccac ccgttcatta cattgccacc gataactatg cgctggttga aagcgcattt
    13021 ttgcatttaa aagagaaagg cgttaaccgc tttgcttttt atggtcttcc ggaatcaagc
    13081 ggcaaacgtt gggccactga acgcgaatat gcatttcgtc agcttgtcgc cgaagaaaag
    13141 tatcgcggag tggtttatca ggggttagaa accgcgccag agaactggca acacgcgcaa
    13201 aatcggctgg cagactgact acaaacgctg ccaccgcaaa ccgggattat tgccgttact
    13261 gacgcccgag cgcggcatat tctgcaagta tgtgaacatc tacatattcc cgtaccggaa
    13321 aaattatgcg tgattggcat cgataacgaa gaactgaccc gctatctgtc gcgtgtcgcc
    13381 ctttcttcgg tcgctcaggg cgcgcggcaa atgggctatc aggcggcaaa actgttgcat
    13441 cgattattag ataaagaaga aatgccgcta cagcgaattt tggtcccacc agttcgcgtc
    13501 attgaacggc gctcaacaga ttatcgctcg ctgaccgatc ccgccgttat tcaggccatg
    13561 cattacattc gtaatcacgc ctgtaaaggg attaaagtgg atcaggtact ggatgcggtc
    13621 gggatctcgc gctccaatct tgagaagcgt tttaaagaag aggtgggtga aaccatccat
    13681 gcaatgattc atgccgagaa gctggagaaa gcgcgcagtc tgctgatttc aaccaccttg
    13741 tcgatcaatg agatatcgca aatgtgcggt tatccatcgc tgcaatattt ctactctgtt
    13801 tttaaaaaag catatgacac aacgccaaaa gagtatcgcg atgtaaatag cgaggtcatg
    13861 ttgtagcgat gatgagaatt gtcggcgtca catcagggaa ctcagcacag ccacctgatg
    13921 cgagcaaaag ccttatccga ccgacaaatc agataaggct cgaggaaatg aaattacata
    13981 tgcgccgcga ttaaccgttg gttatcctgg tacattgcga acaggtagtt gttataactc
    14041 ttccctttgg tcgagtagcc cttcagcttg tgaatcatcg ctgtggcagt cacttcctga
    14101 tccgctttac gcagctgtgc acgcgattta cggaacgaag agtaagccgg gtgcgtattc
    14161 aggttagtga cataggcgct caccgattct ttgacagaac taaactgtga gtaccctttc
    14221 actttacccg gcgcattggt acaacgtcct ttcatgcatt tcatgccgaa cagattgtta
    14281 ttgttgcgcg ccagcttcga cgttccccaa ccgctttctg ctgcagccat cgtcgccacc
    14341 atactggtag ggataatgtc tacgcgttca agcaaggtat tccacgggat ttttcgcgta
    14401 ttaccagacc acttcacctt gtagcgtttg gcgatgtctt tcagacgcgc acgctcagca
    14461 ggtgcccatt ggccctgata ctgttttgaa atcagccagt tacgttctgc agtaatggct
    14521 gcattttggc tggtaatgta aggcattacg gtccggagaa acgccttttt ccttggtgtt
    14581 ccggaagggt attttcgcaa atcaggaagt gaactactct ttgcactatt gcgagaatac
    14641 tcttgtttac tgcttacctg tttattacta gctttagtta agtgggactt ttgactcgct
    14701 gttgttgtgt gcgtcttcgc taacacctca ctcgaaaaca ccagagtgag taacataaga
    14761 atcatcgccc catatcgtcg tatgggagtc aaaatcatca ggtctcctgg tcggatttaa
    14821 tcattccaac accttatatt tttcacaaat ttgagagttg aatctcaaat catatcaaaa
    14881 atagctgtca agagcacccc aaggaatagt ccaaatctga aactatgtca cgagttaacg
    14941 attcagattg gcgctaaatc gcagaaaatg tgggggttat cgcaaaattc agccgttttt
    15001 tgcgcgcgat cgctcaccct tgcttctcat cctgtggact taccgctcag ggatgagttt
    15061 tgtctggctt atcgctggca aactgtctga aatcgcagca ataaggactc atccaccatg
    15121 aaactcgccg cctgttttct gacactcctt cctggcttcg ccgttgccgc cagctggact
    15181 tctccggggt tccctgcctt tagcgaacag ggaacgggaa catttgtcag ccacgcgcag
    15241 ttgcccaaag gtacgcgtcc actcacgcta aattttgacc aacagtgctg gcagcctgca
    15301 gatgcgataa aactcaatca gatgctttcc ctgcaacctt gtagcaacac gccgcctcaa
    15361 tggcgattgt tcagggacgg caaatatacg ctgcaaatag acacccgctc cggtacgcca
    15421 acattgatga tttccatcca gaacgcgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga cgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcgttga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgttct
    20881 cttacggcat gttagaagtt aaccgcctgg caggccgtca gctcccggtc gatggtggct
    20941 ttgatgatga gggcaatttg accaaagaac ctggcgttat cgagaagaat cgccgcattt
    21001 tgccgatggg ctactggaaa ggttctggca tgtcgattgt gctggatatg atcgctactc
    21061 tcctttccga cggcgcatcg gttgccgaag tcacccagga caacagcgac gaatacggca
    21121 tttcacaaat ttttattgcc attgaagtgg acaagcttat cgacggtccc acccgcgatg
    21181 ccaagctgca acgtatcatg gattacgtta ctaccgctga acgcgctgac gaaaaccagg
    21241 ccatccgctt acccggccac gaatttacta ccctgctggc cgaaaaccgc cgtaacggca
    21301 tcactgttga tgacagcgtg tgggccaaaa tccaggcgtt atgaggagat aagtcatgat
    21361 ttttggacat atcgcgcagc ccaatccgtg ccgtttgccc gccgccattg aaaaggcgct
    21421 cgattttctg cgcgctacca acttcaacgt cctggagccg ggcgttgtcg aaatcgacgg
    21481 caagaatatt tatgcgca
//
LOCUS       DAJTRD010000065        21269 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTRD010000065 DAJTRD010000000
VERSION     DAJTRD010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.065"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..448)
                     /locus_tag="OO315_002680"
     CDS             complement(<1..448)
                     /locus_tag="OO315_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5891535.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRIPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLV"
     gene            complement(503..1354)
                     /gene="manZ"
                     /locus_tag="OO315_002681"
     CDS             complement(503..1354)
                     /gene="manZ"
                     /locus_tag="OO315_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5891536.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1367..2167)
                     /gene="manY"
                     /locus_tag="OO315_002682"
     CDS             complement(1367..2167)
                     /gene="manY"
                     /locus_tag="OO315_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5891537.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(2230..3201)
                     /gene="manX"
                     /locus_tag="OO315_002683"
     CDS             complement(2230..3201)
                     /gene="manX"
                     /locus_tag="OO315_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5891538.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3664..5214
                     /gene="yoaE"
                     /locus_tag="OO315_002684"
     CDS             3664..5214
                     /gene="yoaE"
                     /locus_tag="OO315_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5891539.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(5218..6816)
                     /locus_tag="OO315_002685"
     CDS             complement(5218..6816)
                     /locus_tag="OO315_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5891540.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6947..8311)
                     /gene="sdaA"
                     /locus_tag="OO315_002686"
     CDS             complement(6947..8311)
                     /gene="sdaA"
                     /locus_tag="OO315_002686"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5891541.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8495..9073)
                     /locus_tag="OO315_002687"
     CDS             complement(8495..9073)
                     /locus_tag="OO315_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5891542.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(9077..10438)
                     /gene="pabB"
                     /locus_tag="OO315_002688"
     CDS             complement(9077..10438)
                     /gene="pabB"
                     /locus_tag="OO315_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5891543.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10512..10691
                     /locus_tag="OO315_002689"
     CDS             10512..10691
                     /locus_tag="OO315_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5891544.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10811..11170)
                     /locus_tag="OO315_002690"
     CDS             complement(10811..11170)
                     /locus_tag="OO315_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5891545.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11532..11876)
                     /locus_tag="OO315_002691"
     CDS             complement(11532..11876)
                     /locus_tag="OO315_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5891546.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            12008..13918
                     /locus_tag="OO315_002692"
     CDS             12008..13918
                     /locus_tag="OO315_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5891547.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQIVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13976..14671
                     /gene="tsaB"
                     /locus_tag="OO315_002693"
     CDS             13976..14671
                     /gene="tsaB"
                     /locus_tag="OO315_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5891548.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14711..15292
                     /gene="yeaY"
                     /locus_tag="OO315_002694"
     CDS             14711..15292
                     /gene="yeaY"
                     /locus_tag="OO315_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5891549.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15498..17183
                     /gene="fadD"
                     /locus_tag="OO315_002695"
     CDS             15498..17183
                     /gene="fadD"
                     /locus_tag="OO315_002695"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5891550.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            17265..18380
                     /gene="rnd"
                     /locus_tag="OO315_002696"
     CDS             17265..18380
                     /gene="rnd"
                     /locus_tag="OO315_002696"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5891551.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18434..19399)
                     /gene="yeaX"
                     /locus_tag="OO315_002697"
     CDS             complement(18434..19399)
                     /gene="yeaX"
                     /locus_tag="OO315_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5891552.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19455..20072)
                     /gene="yeaW"
                     /locus_tag="OO315_002698"
                     /pseudo
     CDS             complement(19455..20072)
                     /gene="yeaW"
                     /locus_tag="OO315_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20414..20980)
                     /locus_tag="OO315_002699"
     CDS             complement(20414..20980)
                     /locus_tag="OO315_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5891553.1"
                     /translation="MPPEIMRFKERLEEAMNGESSRAFAAKCGLSDGVIRNYLSGKTY
                     PSLDRLAQIAYATGRPIEWFIQSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELL
                     EDKELHSAIELFRKKGFEALMPEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRK
                     KILSKYGIYEQEGLVAPSQEPQDVKKAV"
BASE COUNT         5263 a         5272 c         5464 g         5270 t
ORIGIN      
        1 atacgagaag cttataaatt ttttccagat cgtcgatatt tcgaacgcgg attaacagcc
       61 gacgctgttc taattgcatc accaacacgc catcttccga caagttcatc gctttgattc
      121 ggctatattc aatccagaca ttggcgaaga aaaaaccttt ttgtttaaag atgatcttcg
      181 gaatgcggat ccagaatata taaaaaccca tcagagccag tgcacttaat aaccatgttg
      241 ttattagcgc gccatgattc gtgacgttgt tatagataag aatgacaatc aatccgacga
      301 agataacgct atcgatgcga ccacgccgga gcaaaggaat tgccagcagg gtggggccgt
      361 tacggcgggg catgatgaac tgatcgtaga tcgcgaaggc caggagtgcg gcgatgaaaa
      421 gaatcagtac caggtccgtg attgtcatta atcctccaga taaaaaaacg gggccaaaag
      481 gccccggtag tgtacgacag tcttacagtc ccagcaggcc gcaagcgtaa ccagcgatac
      541 cgatgacgaa gaagccaacg atgatccaca gcgggttaac ttttttgcgc agcagccaca
      601 tacaagcaaa tgtcagcagc agtggtacaa ggcctggcat taactggtca agaatagttt
      661 gcacggtggt gacacgggtc tgtccatcct gcccggtgat ggtcgaaacc accagcggga
      721 tgttgacatg tgtccacttg ttaaccaatg cccccatgac aaacaggccg aggatagacg
      781 ccccttccgt cagtttttgc aggaagccac cacccatatc tttaacgata tcgatacctt
      841 tggagtaacc atacgctacg ccgtagtaac gggttgccag acgcaccagg ttaaacagga
      901 tgaagaacag cagcggacct aacaggctgc cgctcatcgc gataccggca cccagtgctg
      961 caaataccgg acgtacggtt ccccagaaga tcgggtcgcc tacaccagcc agcggcccca
     1021 tcaaaccgac tttgataccg ttgatagcac cgtcatcgat ctctgcgcca ttagcacgct
     1081 gttcttccag cgccagggtt acgccgagaa tcggtgcagc cacgaacggc tgggtgttaa
     1141 agaactccag gtgacggcga atagcttgtt tacgagcttc gttgttctca gggtagaggc
     1201 gacgaattgc cggtaccata gagaagcaga aacccagtgc ctgcatacgt tcgaagttcc
     1261 atgaaccctg gaagaggtta gaacgcagga atacgccacg aatatcactt tgagtgagtt
     1321 ttttctcggt ggtagtttga gttgtatcaa ccatttcgct cacctgttag tccagttcgt
     1381 tatcgagatc gttgttacca gctgcctgag caggcgcacc ggctacgcgg ttgtatttcg
     1441 ggctaagttg gatgtagagc actgccataa cagtaccaat cacacccaga gcaaccaggt
     1501 taaagttggt gaatgctgcg gttacgaagc cgaggtagaa gaacggcatc aggtagccag
     1561 cacgcatcat gttgataacc atcgcataac caaccaccac gatcatgccc ccggcgatat
     1621 tcagaccatt ggtcaccact tccggaatcg cattcagcat gttctgtact tcgctggtac
     1681 caacagacag cgcaacgatg acggccggaa tagccacacg cattgcttgc aggaacagag
     1741 aagaaacgtg gatccaggaa atcgctgtca ggttgccgtt atcagcagcc ttatccgcag
     1801 cgtgctggaa agcaacggta atagtacgaa cgatgatggt cagtacctgg cccgcagcgg
     1861 ccagagggat tgccagtgcg atacctgcac caatgctctg atgacctgcg ataaccagaa
     1921 tggtagaaat gatagaagcc agagcggcgt caggcgcaac tgcagcaccg atgttcatcc
     1981 agcccagcgc gatcatttcc agcgtaccac cgataataat accggttttc atatccccaa
     2041 gaacgatacc caccagggta cacgcgatta gcggacggtg aaactgaaat tcatcgagga
     2101 ttgatcccat acctgcgata caggctacga taaataccag cacaatttga agagtggtaa
     2161 tctccattgt acttctcctg tttacgtaag acttaagtgt gaaaactgaa tgataatcaa
     2221 cacaatacgt tacttatcga ttttgctgat cagatccatc attttcagtt tcggatcggt
     2281 ggaaacctta cggacttcca gctcaatacc gcgtgcattc agttttttga acgcctcgat
     2341 atctttttca tcaaccgaaa ccgcgttatt cacctgggtt ttaccctgac ggaatgccat
     2401 accaccgacg ttaacagagg tgattttcac gccgccttca acgagacgct ctacatctgt
     2461 tgggttggta aataacagca ttacgcgttc gccagcatat ttcgggttgt tgtagacgcg
     2521 aatcattttg gcaacatcaa ctacgtgtgc tgttacgccc ggaggtgcaa cctgggtgag
     2581 cagtgtctta cgaacggtat ccgcagccac ttcatcacta acaacaataa tacgggagac
     2641 attggtttct ttggtccagc gggtggcgac ctgaccgtga atcagacggt cgtcgatacg
     2701 cgcaaggcca ataaccatgt agtcgtttgg ccccattggt tttgccggag ttggagccgc
     2761 ttttggtgct gcggcagcgg gtgctggcgc ggctttttca accggtttgg ctttcagtgc
     2821 tttcacgcct tcacggcctg tttctactgc cagcgcaacc agttcatcaa agctcgggtc
     2881 atcatcacgg gccattaacg tttccacgag cattggaatg ttaacgcctg caatgacttc
     2941 ataatgctct ttgtcgacga caatgcggct ggcagcattg aacgggctgc ctccccatgt
     3001 atcaacgaga aacagcacgc ctttagtggt gtcgagtttt gccaactgag cgttgtattt
     3061 ttcaatcagc gtttcggcat tttcacctgg aacgaaatcg atccagccga cgttttcctg
     3121 ctcgcctaac agcatttctg ccgttttaag caactgctct gcagcccaac catgtgtgcc
     3181 tataacaata gcaatggtca cttgctacct cctttattat cgttaacacc tcaacgtgcc
     3241 agatgtattt ttgaatcgtc gtccacaatc gaatcgattc agataagggc tacaaccaaa
     3301 aacccctgcg tttcgcgatt tattttagat atcgaaaaaa ttattttatg tgatgaagat
     3361 ccgtaattta actttcgatt atcagaaatt tcgaaaggta aaatatcctt gtcacattcg
     3421 tttgcaaagg aaggtaaatc tttgccaaat cagaggcgtc tctgatatgt ttaactcccg
     3481 tttaacaacc atggagtata gggcagtagc ccgcagtatg gatcgtcacc gacgtcattt
     3541 cagcatcagg ccttttaacg cctgcctttc tggcactcta tgccgcacct ttcgtttgca
     3601 ttttgtcgtt acgcctgcat tatttctggc gtcgaatagc tattccttaa gcaggagctt
     3661 gtcatggaat tcttaatgga cccctcaatt tgggcggggc tactcacgct tgttgttctc
     3721 gaaattgtgc tgggtatcga taacctggtc ttcatcgcca ttcttgctga caaactgccg
     3781 ccaaaacaac gcgataaagc gcgtttgctg gggttatcac tggcgctgat tatgcgtctg
     3841 gggctgctgt cgctgatttc atggatggtc acgctgacca aaccgctatt taccgtcatg
     3901 gatttctcct tctccggacg cgatctgatt atgttgttcg gggggatatt cttgctgttc
     3961 aaagcaacaa ccgaactgca tgaacggctg gaaaaccgcg atcatgattc cggccacggt
     4021 aaaggctacg ccagtttctg ggtggtcgtc acacagattg tcatccttga cgccgtcttc
     4081 tcgttggatg cggtaattac tgcagtaggg atggttaacc atctgccggt gatgatggcg
     4141 gcggtagtga ttgcgatggc ggttatgttg ctggcatcga aaccgctgac gcgattcgtt
     4201 aaccagcacc ccacggtggt ggtgctctgt ctgagcttcc tgttaatgat tggtctgagt
     4261 ctggtggcag aaggtttcgg tttccacatt ccgaaaggtt acctgtatgc cgcgattggc
     4321 ttctcgatca ttatcgaagt gtttaaccag attgcgcgtc gcaactttat tcgtcaccag
     4381 tcgactttgc cgctgcgagc gcgtactgcc gatgccatcc tgcgtttgat gggcgggaaa
     4441 cgtcaggcca atgttcagca cgatgccgat aacccgatgc cgatcccgga aggtgcattt
     4501 gccgaagaag aacggtacat gattaacggc gtactgacgc tggcgtcgcg ttctctgcgc
     4561 gggatcatga cgccgcgcgg tgaaataagc tgggttgacg ctaatctcgg ggtcgatgaa
     4621 atccgcgagc aactgctctc ttcaccgcac agtctgttcc cggtatgtcg cggtgaactg
     4681 gatgaaatca tcggtatcgt acgtgctaaa gaactgctgg tggcgctgga agagggcgtt
     4741 gatgtggcgg cgattgcttc ggcgtctccg gcgattatcg tcccggaaac cctcgatccg
     4801 atcaacctgc tgggcgtgct gcgtcgcgct cgcgggagct ttgttatcgt gaccaacgag
     4861 tttggtgtgg tacaaggctt ggtcacgccg ctggatgtgc tggaagccat tgcgggtgaa
     4921 ttcccggacg ctgacgaaac gccggaaatc attaccgatg gtaacggctg gctggtaaaa
     4981 ggcggtacag atttgcatgc cttgcagcag gcgcttgatg ttgagcacct tgccgatgac
     5041 gatgatatcg cgacggtcgc gggcctcgtg atctcggcaa atggtcacat tccccgtgtg
     5101 ggcgatgtga ttgatgtagg gccactgcat atcaccatct ttgaagccaa tgattatcgt
     5161 gttgatctgg ttcgcattgt taaagagcaa ccggcgcacg atgaagatga gtaagcgtta
     5221 acgtaacgac ataatgggcg tgatatgtcc attatgccgg gcgggcggcg gttggctgcc
     5281 cgccagccat ttgggaaaat cacgtagcgg catcggctgt gcgtacaaat acccttgcaa
     5341 aatatgcacg ccatgacggc gcaaatactt cgcctgttct tgcgtttcca cgccctccgc
     5401 caccagttca atattcagtc tttgccccag cgcgatgatg atatcggtca ccgtcgagtt
     5461 aaccgcgtca gaacctatag ctgcggtaaa tgacttatca attttcagca cgtcaggacg
     5521 taatgtttca agccaggaaa acgagctgtt gccggtgccg aagtcatcaa tcgccagttt
     5581 gacgttttta cgatgcagct cgcgggcaat ccgataatca acatccagta aggcatcgcg
     5641 ttcggtgatt tcgaggatca gttgctgaat cgggtgagcg ctaaaccagt actgattgag
     5701 atcttttatc agcacaccac ggcgaaaatg gctgggtgcg acgttaatgc caacatgaaa
     5761 ctgactactc atcgggaaaa catggtgctg acgaatggtt tctgccatca catagtgggt
     5821 cagtggcaca attaaatgat gttcttccgc gataggaata aacacatccg gtgaaatcca
     5881 gccctgacgc ggattattcc agcgcagcaa aatctctaca ccaatacatt gctggctgcg
     5941 cgcattaagc aaaggctgac agaacaattc gaactcatgt tgcgccaggc ccagattaat
     6001 ttcgcgggaa aagctcatcc ggtaagcggt cgccagccag gcgatatagc ccaccagtaa
     6061 acttagcagc accgccagcg gtaattgtgt tggaagatag tgccatgcca gcgccgtagc
     6121 ccccggacca tttacgttaa tggtaaaagg aaagctttgc gaagaaacct gatagttttc
     6181 attgtcttca ggttgcggaa gggaatctac cagcccatta ccatagagca aatgccgttt
     6241 gtccaccgtc aggctggcgc tactgatttg cggcagttgt ggctcaagga gcattgccgt
     6301 cagtaagtcg atgttaatca tctctatgac cccagcattg ctactgcccg ctgcaggcgt
     6361 ccattgaatc aaaaccggac tgcctttaat cagggcacgg tcgatcgtca ggcgtaaaag
     6421 tggttgcggt gcaggaagtt cagccagaat gtccacgacg ggcacattgc gataaccaaa
     6481 aatgctggag caataaagtg tgccgtcttg caccaggcca atggatcgca cagtttggag
     6541 ttttgccgcc tgtttacgta atggcaaatg ggcgacagag cagggcagac caatcagcgg
     6601 aagcaagact ttgctacctg cctgtagggg aagcagtact ttatctaatt cctctacagc
     6661 gtgattggcg aattgtacta cccgttgttg atttaagtta cgctgtgaaa taaatcgcac
     6721 gctcagggtc gaagcgagcg taacgagtgc gcaaatcgta caaacaatca ttcgattacg
     6781 gcgataggtt ttaatgatcc gttgtgcttt ttgcatgcgg gccaccctgt aagcgtatgg
     6841 cgacaaacgc ctgacgcaac agtggaagtg tagtggggaa tattacagca gacgagaaag
     6901 cgggtataaa ttcgcccatc cgttgcagat gggcgagtaa gaagtattag tcacactgga
     6961 ctttgattgc cagaccaccg cgtgaggttt cgcggtattt ggcgttcatg tccttaccgg
     7021 tttcgtacat cgtttcgatg accttatcca gcgagacgcg cggtgcactg gtgcggcgca
     7081 gagccatccg tgcggcgtta atcgccttca cagaggcaat ggcattacgc tcaatgcacg
     7141 gcacctgaac ctgccctgca accgggtcgc aggttaaacc aaggttgtgt tccatgccaa
     7201 tttccgccgc cacgcaaacc tgttccgggc taccgcccag cagttctgca agacccgcag
     7261 cagccattga acaggcaaca cccacttcgc cctggcaacc aacttccgca ccggaaatag
     7321 aggcgttcat tttatacaat gcaccaatcg cgcccgctgc cataaagtaa cgggtataga
     7381 tgtctgggct gaccgattca ataaagtggt catagtaagc cagcactgcc ggaacgatac
     7441 cgcaggcacc gttggttggc gcagttacca cacgaccacc ggcggcgttt tcttcgttaa
     7501 ctgccagcgc aaacatgttt acccagtcaa tgacattcat cggatcgtta gacagtttat
     7561 cgctggaaac taacatccgg cgcagggcag acgcacgacg tggcacgcgc agcgggcctg
     7621 gcagtacacc ttcggtgttc atcccgcgat cgatacatgc ctgcatggtt tgccagacat
     7681 gcgcgaaata ctcgtcgatc tctttcttgc tgtgcagcgc cagttcgttc tgcatagcga
     7741 gaccagacag cgaatagccg gtttcattac agtacgcgag cagttcggtg gcagatttga
     7801 acggatacgg cacgctcact tcgttggcag catcctgacc aaagtgttct tcatcgacga
     7861 taaaaccgcc gccgatggaa taataagttt tgctgtagac gacttcatcg ccgttatagg
     7921 cgtggatttg cataccgttt tcatgcagcg gcaggttgcc gttatgaaaa cgcatcccgt
     7981 tgtcgcgcgg gaaatccact tcatgccgtc cctgtgccag cagcagacgt tcgcgctctt
     8041 ttacgtcgcg aataaaaccg ggaatactgt cgatatccac ggtggcaggt tcgttacctg
     8101 caagacccat aataatggcg atatcggtgt ggtggccttt acccgtcagc gacagtgaac
     8161 cataaacgtc cacggcaacg cgagtaacgc tatccagtaa gcctttttcg accagatcat
     8221 cgacgaactg tttacccgcc ttcataggcc ctacggtatg ggaagatgag ggaccaatcc
     8281 ccaccttaaa catgtcgaat agactaatca cgataatact cctgacaagg tgactggact
     8341 tccagtaacg aactaataac agcgcatagt gtaagaggga acgcggcgac tggcttaact
     8401 attcacatga attaaactaa tcaaaaaccg cgatttaact aatattgcga tgattgtctc
     8461 aagtagtgtt ttgtagatgt aaagataagt atagtcaggg ggtcacacca atttgcagcg
     8521 ccagctcacg aattatgcct gcggtcattc cccatacaaa atactgttcg taccaggaca
     8581 gccatacccg atgtgaatcg ccacggcggt agatatctaa agggtgataa cgacccagat
     8641 gtaatgcctg ggcgagcggc atttcaaaca ccgccgagac ttcatcttca ctggcgcgat
     8701 acggcagatc gggcgggata atgccgacca ctggggttac ttgatagcca gttacgctat
     8761 cgacgggcgg cagcacgccg ataacttcaa cggcggaagg cggtatagcg acctcttctt
     8821 cagcttcgcg cagcgcggcg gcgataactg atgcgtccgt gtcatcgact gcacctccag
     8881 ggaatgccac ttgtccagcg tgtttacgca gatgaattga acgctgagtc agcaacaacc
     8941 ccggttgcgg tcgacggacg atggggatta acacggcagc ctgacgatga tttaggggtt
     9001 cccggttaat ttgcgggcgc aaaagttgaa agcgagataa aaaatcatca agcgtcaggc
     9061 tacggtattc cacgtcttac ttctccagtt ggcgtaatat cttattaact ttatcaaaag
     9121 tttcctgata ttccgcttct tcctggctat cggcgacaat tccaccgccc gcagagcagt
     9181 atatttgtcc gttaatggca gtcagcgtgc ggatagtaat gctggtatcc atgttgccgc
     9241 aaaagctcaa atagccaatg ctgccgcacc aggcattacg tcgctgcggt tccagttcgt
     9301 cgataatttc catagcccgt actttcggag ccccggttat tgagccacca ggaaaagctg
     9361 cgcgcagcag atcgctggcg tgtaactgtt ctggtagtcg cgccgttata gtgctgacca
     9421 gatgatgcac ggcagggaag ggttccacca cgaagagctc tggtactttt accgaaccgg
     9481 ctacggcaac acgaccgata tcattacgca ttaaatcgac aatcatcaga ttttcggcac
     9541 gatctttcgc tgagttcgcc agtttttctg cttgtttgct atcttcctga ggatcgggca
     9601 ggcgtggtag cgtgccttta atcgggcgag tctggatttc actattatca caaagaataa
     9661 accgctctgg cgaaaggctt aaaattgcac cctgttcaag acgtaaaaaa gcgctaaatg
     9721 gcgcgcggtt ggcctgatta agctgaagga atgcctgcca ttcatcgcca gaataggtcg
     9781 catgaaaacg ctgggcgaga ttcacctgat agcaatcacc gctgtgcaga tattcctgta
     9841 cctggcgaaa tttttcgccg tactgctcgt gggtcatatt ggattgccag tcggaagtga
     9901 gcgtgaaatc ttcctgcggc gagaattgct ggctttccag ccaggcccga cgggcattga
     9961 catcattatg actcagcaaa gaaactgtat gacgctggtg gtcgacaatg agcgcccaat
    10021 cgtagatacc cactgccata tccggcagaa cgatatcttg ttccgcaatt tctggcagtg
    10081 actcaaaacg gcggcccaga tcgtagccaa acaaccccag tgcgccgccc tgaaatggca
    10141 aatcttcgtt atgcgttggg cgaatgtctg cgcgatccag cacctgctgg agcacctgta
    10201 gcgggtcatc agtggtcgtt gtgcgttttt cgctttcact aacaacggtt tctttaccta
    10261 aagtggttaa agtgcaaatc ggatcggcga ccacaatatc aaagcggcta tacggatgat
    10321 cggcatagcc ggagtgtaaa agcatcgccc acggcaggtg gcttaagcgg gagaaataaa
    10381 attcagcggc gtcctgacgc cagggtaaag taatcacagt gggagataac gtcttcatca
    10441 gtcctgactc tactggctat gtgccgggta gcatgaaata attagcgcca acaatttagc
    10501 aggagttaac aatgtttgca ggtttacctt cactcatcca tgaacagcag caaaaagctg
    10561 tcgagcggat ccaggaactg atggcacaag ggatgagtag cggccaggca atcgcgctgg
    10621 tggcagaaga gctgcgtgcc aaccactccg gcgagctaat cgtggcgcgt tttgaggatg
    10681 aagacgagta acacactaat ccgtatcgat ttcgttatag aagccgatga tcatttgatt
    10741 gtgagaaaaa ggacgctttt tatatgtgat taaaagcgtc ctgacccatt tatgctgata
    10801 taaatttaac tcagaccagt gcttttagtt cttcctgcat atatgttgaa aagtattcag
    10861 gatttttaat cacagagcgt tcacctgtct ccattttttt tgcccatcca accatttttt
    10921 ccgtgaaccc agggttccag ccttcctggt cagctcgcgc cgtaatatca gcggcactcg
    10981 cgggtactcc cagttctttt aaatatttaa agattccctt cgccgtgctt tcattcatcg
    11041 aacttggtgt tggcgctgat acatccatgg caccaatgaa atccagggct ttatctatta
    11101 cagcaggcat attcttatcc ttaaaattaa ccatgttatc gataaggcta tatatgacct
    11161 taatttgcat ctaatatcaa agggattata acgatatggc acgagttaac ctgttgacat
    11221 tcaggtgttt taaaggtagc agctcatggt gtgttatcca gtaagatgga gaaaattaac
    11281 ggtcagcccg cacgctataa ccagattgtt cagctaagaa agaaaaccag agttgctaat
    11341 caaagggcga acacaacggc atggttatgt ttatttatat agttagtttt atagataaaa
    11401 ttgaaattat aaatataaat aatgaattag cagaagcatc aacagtaata tcttattctc
    11461 tggtcgattt atgtaaaacc gaccagtatt atcgataaat tatcgtctga cttattttgc
    11521 ttcgataaag cttacaccgc agccacaatc ttaatttcaa ctttatactt cgggttcatc
    11581 aaacccgctt gtaccgtgca gcgcaccggc gcatgacccg cgacaaccca ggcatcccac
    11641 gctttattca tcgccgcgaa gtcgttttta tcggccagga aaatggtggc atccagaatg
    11701 ctcgatttat tgctgccctg tttttccagc acggcgtcaa tctgtgccag cgtgttggcg
    11761 gtttgctcaa aggcatcggc gtcgaggttt tccggtacac cagtgtagta gagcgtgttg
    11821 ttgtggatta ctacatcaga ccagcgggct tcagcatcga tacgaacgat agtcatcatt
    11881 ttgtcctcat tatacttcca ttttcgtgct gcaaagactg ccacaatgtg aggttgccgt
    11941 caccacttca actggcgaaa gcgccctcat cctgacataa tccctgttca aatcaacagg
    12001 gggtagtgtg acggacgatt ttgcaccaga cggtcagctg gcgaaagcga taccaggctt
    12061 taagccgcga gaaccacagc gacagatggc ggtagccgtc acccaggcga tagaaaaagg
    12121 ccagccgctg gtggtggaag caggaaccgg tacgggcaaa acctacgctt acctggctcc
    12181 tgcgctgcgg gcgaaaaaga aagtcattat ctcgaccggc tcaaaagcgt tgcaggatca
    12241 gctctacagc cgcgatttgc caacagtctc aaaggcattg aaatatacgg gcaacgtggc
    12301 gctgctgaaa gggcgctcaa actacctctg cctcgaacgt ctcgaacagc aggcgctggc
    12361 ggggggcgat ctgccggtac aaatcttaag cgatgtgatc ctgctgcgct cctggtctaa
    12421 tcaaatagtc gatggtgata tcagcacctg cgtcagcgtg gcggaagatt cacaggcgtg
    12481 gccgctggtc accagcacca acgacaactg tcttggcagc gactgcccga tgtataaaga
    12541 ttgctttgtg gtcaaagcac gtaaaaaagc gatggacgcc gatgtggtgg tggtaaacca
    12601 tcatctcttt ctggcggata tggtggttaa agagagtgga tttggcgagt tgatccctga
    12661 agcggacgtc atgatcttcg acgaagccca ccagctaccg gacattgcca gccagtattt
    12721 tggtcagtca ctctccagtc gacaactgct cgacctggca aaagacatca ccatcgccta
    12781 ccgcaccgaa ttaaaagaca cccagcagtt acaaaagtgc gctgatcgtc ttgcccagag
    12841 tgcgcaggat tttcgtctgc aactcggtga gccaggttat cgcggtaacc tgcgtgagct
    12901 gttagctaat ccgcaaattc agcgggcatt tttactgctc gatgacacct tggaactttg
    12961 ttatgatgtg gcgaaactgt cactggggcg ttccgccttg ctggatgcgg catttgagcg
    13021 cgccacgttg tatcgcacac ggctgaagcg gctaaaagag atcaatcagc cgggctacag
    13081 ctactggtac gaatgcactt cgcgccattt tactctggct ctcacgccgc tcagcgtggc
    13141 ggataaattc aaagagttaa tggcgcaaaa gcccggtagc tggatcttca cctcagcaac
    13201 gctgtcggtg aacgacgatc tgcatcattt cacctcgcgg cttgggatag aacaggcgga
    13261 gtcgttgcta ttgccaagcc cgtttgatta cagccgccag gcgttactct gtgtgccgcg
    13321 caatctgccg caaaccaacc agccaggttc tgctcgccag ttagcggcaa tgctgcgacc
    13381 gatcatcgaa gctaacaacg gtcgttgttt tatgctttgt acctcgcacg ccatgatgcg
    13441 cgatctggcc gagcagttcc gcgctaccat gacgcttccc gttttgttgc agggggaaac
    13501 cagtaaaggc caactgttgc agcagtttgt cagtgccggt aatgcgcttc ttgtggcaac
    13561 cagtagtttc tgggaagggg tggacgtgcg tggcgataca ttgtcattgg taattatcga
    13621 caaattgccg tttacctcgc cggatgatcc actgttaaaa gcgcgcatgg aagattgtcg
    13681 tttgcgcggt ggcgacccgt tcgatgaagt gcaactacca gatgccgtca ttactctcaa
    13741 acagggggta ggtcgactga ttcgcgacgc cgacgatcgt ggcgtgctgg tgatttgtga
    13801 ctatcggctg gtgatgcgtc cttacggcgc gacgtttctc gccagtctgc cgcccgcgcc
    13861 acgcacccgt gacattgccc gtgcggttcg tttccttgcg ataccatcct ccaggtaatt
    13921 tgttacggag tatggtatga tgcgcgcctt tttattgatc taagcacgaa gacccatgcg
    13981 aattctggct atcgataccg cgacagaggc ctgctctgtc gccctgtgga acgacggtac
    14041 tgtcaacgct cattttgagc tttgccctcg tgaacatact caacgaatct taccgatggt
    14101 gcaggatatc ctgaccacca gcggaacttc cctgactgat attaacgctc tggcttacgg
    14161 gcgcggcccc ggtagcttta ccggcgtgcg cattggtatt ggcatcgcgc aagggctggc
    14221 gcttggcgcg gaattaccga tgattggcgt ctccacgcta atgacgatgg cgcaaggggc
    14281 gtggcgcaaa aacggcgcaa cacgcgtgct gtcagccatt gatgcgcgaa tgggcgaagt
    14341 ttactgggcc gaatatcagc gtgatgaaaa cggtatctgg cacggtgaag aaaccgaagc
    14401 cgtactcaaa cccgaactcg ttcatgaacg aatgcaacag ctttccggcg aatgggtgac
    14461 ggtagggacg ggctggcaag cctggccgga tctcggtaaa gagagcgggc tggttttgcg
    14521 cgatggcgaa gtgttactcc ccgctgcaga agatatgctg ccgattgctt gtcagatgtt
    14581 tgccgagggt aaaacggtgg cggtggaaca tgccgagccg gtttatttgc gtaacaacgt
    14641 cgcatggaag aaacttccgg gcaaagaatg aatcttaaga gtatgccctg agaaaaaaag
    14701 gagtcgcatc atggcggttc aaaagaatgt tatcaaaggc atactggcag gtacgtttgc
    14761 gctaatgctg agcggttgtg tcactgtgcc ggacgccatt aaaggcagca gtaccacgcc
    14821 gcaacaagat ttagttcggg tgatgagtgc gccgcagctg tacgttggtc aggaggcacg
    14881 ctttggtggc aaagtggttg cggtacaaaa ccagcaaggg aaaacccgcc tggaaattgc
    14941 taccgtaccg ctggacagcg gagctaggcc gacgctggga gaaccttctc gcggtcgcat
    15001 ttatgccgat gtgaacggtt ttctggaccc ggtggatttc cgtggacaac tggttacggt
    15061 agtcgggcca atcaccggtg cggttgacgg caaaatcggc aatacgccct ataaatttat
    15121 ggtgatgcaa gtaacgggtt acaaacgttg gcatttaacc cagcaggtga ttatgccgcc
    15181 tcagccgatt gatccatggt tttatggcgg tcgtggctgg ccctatggct acggcggatg
    15241 gggctggtat aatcccggcc ccgcgagagt acaaacagtt gtaaccgaat aattgcttgt
    15301 ttttaaagaa tgcagaaaca gcggctggtc cgctgtttct gcattcttac ggtaaagata
    15361 aaaataaata gtgacgcgct tcgcaacctt ttcgttgggt aattatcaag ctggtatgat
    15421 gagttaatat tatgttaacg gcgtgtatat catttggggt tgcgatgacg acgaacacgc
    15481 attttagagg tgaagaattg aagaaggttt ggcttaaccg ttatcccgcg gacgttccga
    15541 cggagatcaa ccctgaccgt tatcaatctc tggtagatat gtttgagcag tcggtcgcgc
    15601 gctacgccga tcagcctgcg tttgtgaata tgggggaggt gatgaccttc cgcaagctgg
    15661 aagaacgcag tcgcgcgttt gccgcttatt tgcaacaagg gttggggctg aagaaaggcg
    15721 atcgcgttgc gttgatgatg cctaacttat tgcaatatcc ggtggcgcta tttggcattt
    15781 tgcgtgccgg gataatcgtc gtaaacgtta acccgttgta taccccgcgt gagcttgagc
    15841 atcagcttaa cgatagcggc gcatcggcga ttgttatcgt gtctaacttt gcccacactc
    15901 tggaaaaagt ggttgataaa accgccgttc agcacgtaat tctgacccgt atgggcgatc
    15961 agctatctac ggcaaaaggc acggtagtca atttcgttgt taaatacatc aagcgcttgg
    16021 tgccgaaata ccatctgcca gatgccattt catttcgtag cgcactgcat aacggctacc
    16081 ggatgcagta cgtcaaaccc gaactggtgc cggaagattt agccttcctg caatacaccg
    16141 gcggcaccac aggtgtggcg aaaggcgcga tgctgactca ccgcaatatg ctggcgaacc
    16201 tggaacaggt caacgccacc tatgggccgt tgctgcatcc aggtaaagaa ctggtagtaa
    16261 cggcactgcc gctgtatcac atttttgccc tgaccattaa ctgcctgctg tttatcgaac
    16321 tgggtgggca gaacctgctt atcactaacc cgcgtgatat tccagggttg gtaaaagagt
    16381 tagcgaaata tccgtttacc gctatcactg gcgttaacac cttgttcaat gcgttgctga
    16441 acaataaaga gttccagcag ctggatttct ccagtctgca tctttccgca ggcggtggga
    16501 tgcctgttca gcaagtggtg gcagaacgct gggtgaaact gaccggacag tatctgctgg
    16561 aaggttatgg cctgaccgag tgcgcgccgc tggtgagcgt taacccgtat gatattgatt
    16621 atcatagcgg tagcatcggt ttgccggtgc catcgacgga agccaaactg gtggatgacg
    16681 acgacaacga agtaccaccg ggtcaaccgg gtgagctttg tgtcaaagga ccgcaggtga
    16741 tgctgggtta ctggcagcgt ccggatgcta cagatgagat catcaaaaat ggctggttac
    16801 acaccggcga catcgcggtg atggatgaag aaggattcct gcgcattgtc gatcgtaaaa
    16861 aagacatgat tttggtttcc ggttttaacg tctatcccaa cgagattgaa gatgtcgtca
    16921 tgcagcatcc tggcgtacag gaagtcgcgg ctgttggcgt accttccggc tccagtggtg
    16981 aagcggtgaa aatcttcgta gtgaaaaaag atccatcgct taccgaagag tcactggtga
    17041 ctttttgccg ccgtcagctc acgggataca aagtaccgaa gctggtggag tttcgtgatg
    17101 agttaccgaa atctaacgtc ggaaaaattt tgcgacgaga attacgtgac gaagcgcgcg
    17161 gcaaagtgga caataaagcc tgagcgttaa gtcagtcgtc agacgccggt taatccggcg
    17221 ttttttttga cgcccactaa agagaaaaca atttgaatta ccaaatgatt accacggacg
    17281 atgcgctggc ttctttgtgt gaagccgtcc gtgtctttcc ggcgatagcc ctggatactg
    17341 aatttgttcg tacgcgcact tattacccgc agctggggtt gattcaactt ttcgatggcg
    17401 agcatctggc gctaattgat cctctcggga tcaccgactg gtcaccgctg aaagcgatcc
    17461 tgcgcgatcc gtccatcaca aaatttctcc atgcaggcag tgaagatctg gaagtgttcc
    17521 tcaatgtctt tggcgaatta ccacaaccct tgattgacac gcaaatcctt gctgccttct
    17581 gcggacgccc gatgtcatgg ggtttcgctt ccatggtgga agagtattcc ggcgttacgc
    17641 tggacaagag tgaatcgcgc accgactggc tggccagacc gctgaccgaa cgtcagtgtg
    17701 aatacgcagc ggcggatgtc tggtatctgt taccgatcac cgccaagctt atggtagaaa
    17761 cggaggcctc cggctggcta cctgcggcgc tggatgaatg ccgcctgatg caaatgcgtc
    17821 gtcaggaagt cgttgcgccg gaagatgcct ggcgtgatat caccaatgcc tggcagttac
    17881 gcacacgcca actggcctgt ctgcaactgt tagccgactg gcgactgcgc aaggcgcgag
    17941 agcgtgatct ggcggtgaac tttgtcgtgc gtgaagagca tttgtggtcg gtcgcgcgtt
    18001 atatgccggg aagtttaggc gaactggaca gcctgggttt atccggtagc gaaatccgct
    18061 ttcacggtaa aacgctgctg gcgctggtgg aaaaagcgca gacattgccg gaagatgcct
    18121 taccgcagcc gatgcttaac ctgatggaca tgcctggtta tcgtaaagcg tttaaagcga
    18181 ttaagtcgct gattactgac gtgagcgaaa cgcataagat cagcgccgaa ttgctggcat
    18241 cgcgtcggca aatcaaccaa ctgctgaact ggcactggaa actgaaaccg cagaacaatt
    18301 tgccggagct gatttccggc tggcgtggtg agctgatggc ggaagcatta cacaatttat
    18361 tgcaggaata tccgcagtaa aatcttccga agccggactg ggcgcgctca gcgccacatc
    18421 cggctttggc aaactacaaa tccaacacca ggcgtttacc cttcgcacgc gaacaacaga
    18481 tcaacatact ttgctggctg gcacgctctt catcgctaaa atactgatcc cgatggtcag
    18541 cttcaccttc cagtattgcc gtttcgcagg ttccgcatac cccttcacga cataaacatt
    18601 ccactttcgc ggctttatta ttttcaataa cctgcaaaat agtcatctct tccggcacca
    18661 caaactcttt tccggaacgg gcaagcacca gggtaaatgc atcgccggtt ttgtcttcga
    18721 tagcaaattg ctcaaagtgc agtgtatcgg cggcgatgtc cagacgcgca gcttcacttc
    18781 ttaccgcttc aattagcgcc tcggggccac aggtataaac gtgtgtgcca ggttcgatat
    18841 ccgccaataa tcgcgctaat tccagtcgtg ttccggttga tgaatgatgc aaatggactt
    18901 tctcagcctg cggatgctgg actagctcag cacgaaatgc gcaactttct ggatttcgcg
    18961 agcagtaatg tagctgccag tcgatgtcgc tgtgttgcaa ctctgccatg tgcgacagga
    19021 aaggggtgat accaataccg cccgcgataa acagatgctt tctggctgag ggaattagcg
    19081 caaacaggtt attaggcgtt gagatcgtta accgatcgcc cacttttacc tgctgatgca
    19141 aaaagcggga accgccgcgc gagttttctt ccagtcgaac ggcaatctga taactagagg
    19201 tgttatgcgg tgaactcagt agtgaatacg cattgctgta ctggttatca ccatcgctca
    19261 tctgcactag gatgtgactt cctccggtga acgcaggcag cggtttgcca tcggttgcca
    19321 ccagcgtgaa gcgtttcacc tgttcggtaa ggggttcaac ctggctcacc tgtacttcaa
    19381 acatttgata gtctgacatt gctgtactcc atcaaaaatc agcccagcgg cggaaaatac
    19441 cgccgccgat gtcattagtc cttaaacacc tgcgccagca gattatggaa atgggcgata
    19501 ccatgttcgg aaatgccact accgctactg tcggccatga tgcgcccctg accacgatag
    19561 ccacgcgatt tcagcccttt ctgtacgctt tcaaccagac gtaaatcttc cggatggaac
    19621 acatcgcgat accactcaat cagcgatttt tgctcgtcgg ttaactcttc attggtgaag
    19681 taaatatcgt agttttgcag ggtagtttca gaatccaccg ggaattcata aatgaccgtc
    19741 atcatccctt tgatcggggt gacgttcagc atcgtgcacg gccacagcca gaaaccgtgg
    19801 aatgccgcat ccgtaccctc ttcaaattta aacgactgtt cggacggttt ggcgaaaccg
    19861 tattgcagcg tccagttacc gtgcatggtg tgccagtaac gatcaacctg tacggagtcg
    19921 gagaaaccta gatgcgccgg accacagtga tagcactcga gatagttatc gacaatgttc
    19981 ttccagttgg caggcgtgcg ggtggtaaag cgggccgcta gtttcagatt gtggacttcc
    20041 gggcaggctt ccagcacttt cgcgcccaga ccggtgtatt gattcacttg aagtacgaaa
    20101 aaaaaccggg aggacattgg attattcggg ttctgatggg attagatttg gtggggcttg
    20161 caagcctgta gcgcaaattt tagtcgttaa tcaatgaaac gcgaaagata gtaaaaaatt
    20221 gcttttgttt cattgaaaat acgaaaaaca aaaacactgc aaatcatttc aataacagct
    20281 tcaaaaaacg ttcaaaaccg ataacaacca ggctgtaacc aaatgactca tatcacaaat
    20341 cagctcatgc cgtttaggta tgttacatgt gtgattatgt gaggtgaagt atgttttagc
    20401 tggttcatgg ttgttatacg gcttttttta catcttgtgg ttcttgtgaa ggagctacaa
    20461 gaccttcctg ttcataaata ccatactttg acaaaatctt tttacgagta tctgttggta
    20521 atgattcaag cattttggct gtttgtaagg tttgctgcga aataccttgc aaatcagcct
    20581 ggatggaatc atcggaagaa aaaatctcag gcataagagc ttcaaatcct ttctttctga
    20641 acaactcaat cgcagaatgc agttccttgt cctctaacaa ttcgacaatt gcgatcagtt
    20701 tagttttcaa tgtgctgcgg tcaggttgag aatttttatt ctcttgatta tttttatgct
    20761 caaccattgg catgctctga ctttggataa accactctat tggacgccct gttgcgtagg
    20821 ctatttgtgc taatcgatct aaagacgggt atgtcttgcc agacaggtag tttctgataa
    20881 ccccatcaga aagtccgcac ttagctgcaa acgcccggct tgattctcca ttcattgcct
    20941 cttcaaggcg ttccttgaaa cgcattattt ccgggggcat aaaaatgcgc tctgcatttt
    21001 tgctttcttt ttcacctgtc atgcccaatc tctctgttat ataaagaatt ttacagaatt
    21061 ctttgatttg cactggttaa aataaaaatg cgcatagctt ttgaattgag catttttctg
    21121 cgctatcgtt tttcccgata gcggtagccg gtatcagtta ccgaaaacgt taagagagta
    21181 aggatcgcag aatgtcagca aaaaaccaag actggcacgc agagcagata aaggctgctg
    21241 ttcgtatgaa ggggaagaca ctatctcaa
//
LOCUS       DAJTRD010000066        21253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTRD010000066 DAJTRD010000000
VERSION     DAJTRD010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.066"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            18..791
                     /locus_tag="OO315_002700"
                     /pseudo
     CDS             18..791
                     /locus_tag="OO315_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO315_002701"
     CDS             complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO315_002701"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5891554.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO315_002702"
     CDS             complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO315_002702"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5891555.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO315_002703"
     CDS             complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO315_002703"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5891556.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4989..7223)
                     /locus_tag="OO315_002704"
     CDS             complement(4989..7223)
                     /locus_tag="OO315_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5891557.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO315_002705"
     CDS             complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO315_002705"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5891558.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8629..11385
                     /gene="barA"
                     /locus_tag="OO315_002706"
     CDS             8629..11385
                     /gene="barA"
                     /locus_tag="OO315_002706"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5891559.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO315_002707"
     CDS             complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO315_002707"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5891560.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12978..14318)
                     /locus_tag="OO315_002708"
     CDS             complement(12978..14318)
                     /locus_tag="OO315_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5891561.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO315_002709"
     CDS             complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO315_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5891562.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16106..16555)
                     /locus_tag="OO315_002710"
     CDS             complement(16106..16555)
                     /locus_tag="OO315_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5891563.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO315_002711"
     CDS             complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO315_002711"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5891564.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17355..17684)
                     /locus_tag="OO315_002712"
     CDS             complement(17355..17684)
                     /locus_tag="OO315_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5891565.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO315_002713"
     CDS             complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO315_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5891566.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18919..19767
                     /gene="queF"
                     /locus_tag="OO315_002714"
     CDS             18919..19767
                     /gene="queF"
                     /locus_tag="OO315_002714"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5891567.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19879..21243
                     /gene="ppnN"
                     /locus_tag="OO315_002715"
                     /gene_synonym="ygdH"
     CDS             19879..21243
                     /gene="ppnN"
                     /locus_tag="OO315_002715"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5891568.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5229 a         5670 c         5067 g         5287 t
ORIGIN      
        1 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta
       61 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
      121 cccaagtgca aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat
      181 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      241 tgatcgttgt tgcctagtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag
      301 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      361 aacaacaaaa attagctaag ggattagagt tagctataat cgctttgaat aaccaactca
      421 cttcacaaca ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc
      481 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
      541 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc
      601 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt
      661 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
      721 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg
      781 gccgctggta actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat
      841 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
      901 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca
      961 tagaaccagt tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat
     1021 cttcagtttc accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct
     1081 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     1141 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     1201 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt
     1261 cagattcgtc cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca
     1321 ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt
     1381 taccatcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     1441 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     1501 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     1561 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     1621 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     1681 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     1741 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     1801 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     1861 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     1921 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     1981 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     2041 cagctgccat accgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     2101 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     2161 caagtcacta gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc
     2221 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     2281 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     2341 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     2401 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     2461 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc
     2521 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     2581 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     2641 ccgggtactt acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat
     2701 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     2761 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     2821 cgccaggtac gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat
     2881 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     2941 gtgcttcgat cactgattta taagcatccg gcagttcaat gtacttgccg accataccga
     3001 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     3061 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     3121 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     3181 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg
     3241 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     3301 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     3361 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaacg
     3421 gcaaggattc gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt
     3481 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg
     3541 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     3601 tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt
     3661 ccaagtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     3721 tagtacctgg atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac
     3781 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     3841 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     3901 agacgatgga ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac
     3961 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     4021 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     4081 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     4141 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     4201 ctccacttcg cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc
     4261 tttcgtccct aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc
     4321 ctccagttta gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc
     4381 ttcgtagact ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca
     4441 acgtttctgg attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag
     4501 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     4561 cacttcacta ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa
     4621 tttatcgcta atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     4681 ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa
     4741 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     4801 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg
     4861 cataatagtg agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg
     4921 tcaggtgtaa taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct
     4981 atgtaagact aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     5041 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     5101 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     5161 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5221 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5281 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     5341 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5401 tggtgcatca ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt
     5461 tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     5521 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5581 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
     5641 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5701 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5761 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5821 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
     5881 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5941 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     6001 acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca
     6061 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     6121 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6181 tccgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
     6241 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
     6301 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6361 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6421 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
     6481 tcagctttaa cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata
     6541 tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc
     6601 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
     6661 cggttcgcca acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct
     6721 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6781 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6841 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6901 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6961 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     7021 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     7081 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     7141 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7201 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7261 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7321 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7381 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7441 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7501 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7561 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag
     7621 gaacaccttc tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7681 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7741 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7801 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7861 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7921 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7981 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg
     8041 ccctgacttt gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt
     8101 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     8161 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8221 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8281 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8341 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8401 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8461 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8521 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8581 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8641 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8701 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8761 ggtgctagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8821 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8881 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8941 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9001 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9061 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
     9121 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg
     9181 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9241 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9301 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9361 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9421 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
     9481 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9541 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9601 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9661 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9721 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9781 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9841 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9901 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9961 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10021 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    10081 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10141 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10201 attatcgaag ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca
    10261 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10321 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10381 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10441 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc
    10501 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10561 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    10621 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    10681 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt
    10741 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg
    10801 gatggcattc gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt
    10861 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10921 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10981 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    11041 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11101 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    11161 gaggaacagc tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11221 ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa
    11281 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11341 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11401 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11461 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11521 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11581 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11641 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11701 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11761 acccagcccc ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11821 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11881 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11941 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12001 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12061 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    12121 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12181 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12241 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12301 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12361 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12421 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12481 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12541 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12601 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12661 gcgcccacta gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12721 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12781 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12841 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12901 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12961 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13021 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta
    13081 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13141 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13201 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13261 acatgggtaa atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc
    13321 agcccccagt cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13381 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13441 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13501 acttcacgtc cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13561 tcattcagcc ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13621 acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc
    13681 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13741 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13801 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct
    13861 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13921 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13981 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccaaaggt atcaaaatcg
    14041 gctgcctgat tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg
    14101 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    14161 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca cgatattgcg ggtgaagtat
    14221 gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact
    14281 ttcatatcag taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt
    14341 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc
    14401 atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc
    14461 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14521 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14581 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14641 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14701 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc
    14761 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14821 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14881 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14941 ctgcccccac ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat
    15001 cagcgcgcca cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt
    15061 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15121 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15181 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac
    15241 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15301 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15361 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15421 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15481 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15541 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15601 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15661 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15721 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15781 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15841 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15901 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    15961 agaaataaag aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc
    16021 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16081 acgacattta ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg
    16141 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16201 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat
    16261 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    16321 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    16381 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    16441 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg
    16501 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    16561 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    16621 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    16681 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    16741 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    16801 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatgtcg
    16861 tcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    16921 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    16981 tgctggctgc gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17041 caccagtgga taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17101 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17161 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg
    17221 ctgtcctatc tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    17281 gctacggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    17341 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca
    17401 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    17461 acagcaaaag cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc
    17521 agcccccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    17581 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    17641 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    17701 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc
    17761 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    17821 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    17881 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac
    17941 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18001 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18061 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18121 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18181 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    18241 ttatttcctg gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat
    18301 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaattc
    18361 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    18421 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    18481 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    18541 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    18601 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    18661 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    18721 gttgaaataa tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta
    18781 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    18841 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    18901 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    18961 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19021 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19081 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19141 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag
    19201 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    19261 ctggagcgcg acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt
    19321 gatgaactgg aaggccagcc gataggtcat ttcaatggca cttgcattga tgaccaggat
    19381 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    19441 aaagtagtgg aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat
    19501 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    19561 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    19621 cgcatcttta atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt
    19681 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    19741 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    19801 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    19861 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    19921 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    19981 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20041 ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg
    20101 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20161 gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccataatacc
    20221 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    20281 atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    20341 ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg
    20401 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg
    20461 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    20521 acagagccgt cgattatcgc cgctgaaccg cctaacccac tggtcaacga attgatcatc
    20581 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    20641 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    20701 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    20761 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    20821 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    20881 ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc
    20941 attgcgccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21001 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21061 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21121 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21181 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    21241 taatctcccc tcc
//
LOCUS       DAJTRD010000067        20796 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTRD010000067 DAJTRD010000000
VERSION     DAJTRD010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20796
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.067"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..256
                     /gene="mliC"
                     /locus_tag="OO315_002716"
     CDS             <1..256
                     /gene="mliC"
                     /locus_tag="OO315_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5891569.1"
                     /translation="ERMQTDTLEYQCDEKPLTVKLNNPRQEVSFVYDNQLLHLKQGLS
                     ASGARYSDGIYVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
     gene            315..971
                     /gene="pdxH"
                     /locus_tag="OO315_002717"
     CDS             315..971
                     /gene="pdxH"
                     /locus_tag="OO315_002717"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5891570.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHGQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            1100..2374
                     /gene="tyrS"
                     /locus_tag="OO315_002718"
     CDS             1100..2374
                     /gene="tyrS"
                     /locus_tag="OO315_002718"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5891571.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            2433..3296
                     /gene="pdxY"
                     /locus_tag="OO315_002719"
     CDS             2433..3296
                     /gene="pdxY"
                     /locus_tag="OO315_002719"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5891572.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO315_002720"
     CDS             complement(3340..3945)
                     /gene="gstA"
                     /locus_tag="OO315_002720"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5891573.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO315_002721"
     CDS             complement(4051..5553)
                     /gene="dtpA"
                     /locus_tag="OO315_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5891574.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO315_002722"
     CDS             complement(6164..6799)
                     /gene="nth"
                     /locus_tag="OO315_002722"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5891575.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO315_002723"
     CDS             complement(6799..7494)
                     /gene="rsxE"
                     /locus_tag="OO315_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5891576.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO315_002724"
     CDS             complement(7498..8118)
                     /gene="rsxG"
                     /locus_tag="OO315_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5891577.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO315_002725"
     CDS             complement(8122..9180)
                     /gene="rsxD"
                     /locus_tag="OO315_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5891578.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(9181..11307)
                     /gene="rsxC"
                     /locus_tag="OO315_002726"
     CDS             complement(9181..11307)
                     /gene="rsxC"
                     /locus_tag="OO315_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5891579.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIARS
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDP
                     RKAAVAAAIARVQAKKAAQQKVVNED"
     gene            complement(11300..11878)
                     /gene="rsxB"
                     /locus_tag="OO315_002727"
     CDS             complement(11300..11878)
                     /gene="rsxB"
                     /locus_tag="OO315_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5891580.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(11878..12459)
                     /gene="rsxA"
                     /locus_tag="OO315_002728"
     CDS             complement(11878..12459)
                     /gene="rsxA"
                     /locus_tag="OO315_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5891581.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(12536..12976)
                     /locus_tag="OO315_002729"
     CDS             complement(12536..12976)
                     /locus_tag="OO315_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5891582.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(13062..13277)
                     /gene="ydgT"
                     /locus_tag="OO315_002730"
     CDS             complement(13062..13277)
                     /gene="ydgT"
                     /locus_tag="OO315_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5891583.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(13550..13675)
                     /gene="blr"
                     /locus_tag="OO315_002731"
     CDS             complement(13550..13675)
                     /gene="blr"
                     /locus_tag="OO315_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5891584.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            13918..14958
                     /locus_tag="OO315_002732"
     CDS             13918..14958
                     /locus_tag="OO315_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5891585.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(14994..15995)
                     /gene="add"
                     /locus_tag="OO315_002733"
     CDS             complement(14994..15995)
                     /gene="add"
                     /locus_tag="OO315_002733"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5891586.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(16099..17271)
                     /gene="malY"
                     /locus_tag="OO315_002734"
     CDS             complement(16099..17271)
                     /gene="malY"
                     /locus_tag="OO315_002734"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5891587.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(17281..18873)
                     /gene="malX"
                     /locus_tag="OO315_002735"
     CDS             complement(17281..18873)
                     /gene="malX"
                     /locus_tag="OO315_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5891588.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            19048..20076
                     /gene="malI"
                     /locus_tag="OO315_002736"
     CDS             19048..20076
                     /gene="malI"
                     /locus_tag="OO315_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5891589.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            20188..>20796
                     /gene="hdhA"
                     /locus_tag="OO315_002737"
     CDS             20188..>20796
                     /gene="hdhA"
                     /locus_tag="OO315_002737"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR5891590.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
BASE COUNT         5308 a         5235 c         5335 g         4918 t
ORIGIN      
        1 tgagcgaatg caaaccgata cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa
       61 actgaataat ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa
      121 acagggcctt tcagcctctg gcgcgcgtta ctccgacggg atctatgttt tctggtcgaa
      181 aggtgaggaa gcgactgtct ataaacgcga ccgcatcgtc ttgaataact gtcagttaca
      241 aaatccacag cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat
      301 ccgtaaagaa aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg
      361 aatacaccaa aggcgggtta cgccgccgcg atcttcccgc cgatccatta accctttttg
      421 aacgttggct ctctcaggct tgtgaagcca aactggcgga ccctaccgcg atggtggtcg
      481 ctaccgtgga tgaacatggt cagccttatc agcgcatcgt tttactcaaa cattacgacg
      541 aaaaaggcat ggtgttttac accaacctcg gtagccgtaa agcacatcaa attgaaaata
      601 atccgcgcgt tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg
      661 gtaaagcaga acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg
      721 atagccagat tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc
      781 ttgaaagtaa attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga
      841 gcttttgggg cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc
      901 atcgcctgca tgaccgcttt ttgtaccagc gtgaaaatga tgcgtggaag attgatcgtc
      961 ttgcaccctg aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac
     1021 tttattctat gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt
     1081 tatatacatg gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg
     1141 gctggtagcc caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat
     1201 cgcgctctat tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc
     1261 attgttatgc ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg
     1321 cgcgacgggt ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga
     1381 agaaactgtt caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt
     1441 cgactgtgga gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa
     1501 tgtgctgacc ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa
     1561 agaagcggtt aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc
     1621 ctacaacctg ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg atgtggtgct
     1681 gcaaattggt ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg
     1741 tctgcatcag aatcaggtgt ttggcctgac cgttccgctg atcaccaaag cagacggcac
     1801 caaatttggt aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta
     1861 caaattctac cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt
     1921 cttcaccttt atgagcattg aagagatcaa cgccctagaa gaagaagata aaaacagcgg
     1981 taaagcaccg cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga
     2041 agaaggttta caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc
     2101 gctgagtgaa gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga
     2161 aaagggcgca gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca
     2221 ggcgcgtaaa actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc
     2281 tgaatacttc tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg
     2341 caaaaagaat tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag
     2401 aaatcactcc ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt
     2461 ctcacgttgt ttatggtcat gcgggtaaca gtgcggcaga gtttccgatg cgccgcctgg
     2521 gcgcgaacgt ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat
     2581 ggactggctg cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca
     2641 ttgataaatt acacacctgt gatgccgtat taagtggcta tctgggatcg gcggagcagg
     2701 gtgaacatat cctcggtatc gtccgtcagg tgaaagcagc gaatccgcag gcgaaatatt
     2761 tttgcgatcc ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag
     2821 agtttcatgt gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc
     2881 tggaaatact ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg
     2941 aactcattga gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca
     3001 gccgtgaccg ttttgaaatg ctgctggtca ccgccgatga agtctggcat atcagccgtc
     3061 cactggtgga ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac
     3121 tgctggtgaa actgcttcag ggggcaacgc tgcaggaggc cctggaacat gtgaccgctg
     3181 cagtctacga aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg
     3241 ctgctcagga tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata
     3301 tctcatttcg gcccacaacg acctgtgggc cgatggctat tactttaagc cttccgctga
     3361 cagcgcgtct tgtacttccg gacgttcagc catacgttgc ataaatgccg caatgtgctc
     3421 taacccttcc agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc
     3481 atcagcaatt gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac
     3541 atattgcagc ttcttatcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc
     3601 tgggcgaaac agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc
     3661 gatggtttta tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct
     3721 gtcggcaaga tactgcataa tcgcaacgcc ttccgtcagc aaagtaccat cgtccagcag
     3781 caatgcaggc acctgcccct tagggttaac ggaaaagtaa tcgtcaccgt tttcgagacg
     3841 ttttttcatt aaatccacac tgacgagcgt aaaatccttt ccgctctcac gcagggtgat
     3901 atgggaagcg agagagcagg cgcccggttt gtagaacaat ttcatcggta actccttttg
     3961 tctgtggttt aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt
     4021 tagcggcttg taaaagagtt tccctgaatt ttacgctaca gtagctttcg ccgctttgtc
     4081 tgcagcgtca tcctgcgtca tgcggtgcag tttaggcgcg gtcagcagca tcagtactgc
     4141 aataacggca gtagcgacac caatctgcaa gaatacgcga ccatagactt ccagtgacat
     4201 cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag
     4261 gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg
     4321 aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg
     4381 caggccatag cttgcgacca gccagctgac agacacgata cctgcgtcag acgcgaattt
     4441 cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt
     4501 ggttggcatc ggcagggtat cacccatctt gttatagata gcggccagaa tcggactacc
     4561 gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag
     4621 aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta
     4681 cagcacgaag aagatgattg cttcgagcat caggatgaag gcaacgatca ttttacgacg
     4741 cgcagcacct ttcatcgcga aagcttcttt accgaagata accacaatac cgaaggcaac
     4801 aacgcccagc gccatacgcg caacttcctg attgtgcagt agccaggtgg caacagcgat
     4861 cagcgccaca acaccaataa tggtcagcaa caggttacgg tagttgactg gttcaaagtc
     4921 tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat
     4981 cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca
     5041 cggggtggct atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa
     5101 tgcaccgtcc agacgtgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt
     5161 agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa caataccggc
     5221 gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat
     5281 aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc
     5341 ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag
     5401 ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca
     5461 taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac
     5521 gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat
     5581 taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga
     5641 ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc
     5701 tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa
     5761 tttttcaaaa aacgtaacgg cagatttttt cactgcattg agagggggat gatggcttgg
     5821 cggcgatatg ttctgctatt tagcgttttt cctagtgaga tagtccagtt tctgaaaaat
     5881 agccagtgta atgttttgca ggtcaatatt tgttgcgtgt gtaaatttaa atcattttaa
     5941 cctgattctt gcaatgtttt tacattgcca cagcatcgtg atcttgatca cgaatgaata
     6001 gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc aaacggatta
     6061 cctgatgata acgctaagca atggcatcat tgacgttaaa aagatgtttt aaaagaggat
     6121 aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg tcaactttct
     6181 ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg cgggcaatgc
     6241 aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac tctgccggaa
     6301 ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca aattgagtac
     6361 gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg aatgcagtgt
     6421 ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc gcagcacgat
     6481 cttccggaac ctcgccatta tgttgttcca gtaatatacg gcaggttttg atgatatttt
     6541 ctgctttgct gttataaagg ccaatcgttt tgatataggt tttcacccct tcaacgccta
     6601 gttcaagcat cgctgcaggc gtattcgcca ccgggtagag tttcgccgtc gccttattaa
     6661 cactgatatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca aaaggcgaac
     6721 tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg aggatctcca
     6781 gacgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg cacgttcggc
     6841 agctgcttca gcacggcgct ttttcattct ttcatcaatc aggtattttc ctgccagcat
     6901 cagtcccaag ccaataaatg cacctggtgg cagcatcgcc agcaggaaag gggagtcggt
     6961 gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat ctgcaccgtc
     7021 aaacaacgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca tggcgcaggt
     7081 tgcgcccgta ccaattgaaa agccgtccag tgccgaaagc gccggacctt ttttggcggc
     7141 gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa taaaaatccc
     7201 taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag cgctgaccac
     7261 cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc agtgacgcag
     7321 cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc ctaaacccag
     7381 agcgttagtg gcagtggatg tgaccgccaa tagcggacaa aggccgagca gttggaccag
     7441 tgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc tcacgcttta
     7501 ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt acaatccggc
     7561 gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga actggtcgaa
     7621 atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga ttttttttcc
     7681 cgcaaaatgg gtgagccagt cagaaaggcg caattcgatt ttatcgccaa gccctggcgt
     7741 ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgcca ttaaaatcgg ctcccaccag
     7801 cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg cggctaccgg
     7861 tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact ctggcgcagt
     7921 taccagatag caactctgtg cgagcgcatt gttatagcgt tcggctggca gcacctgatc
     7981 aaataacgtc ttttgttgca gactggcctg ttcagcaatt gtcgttttgg tcatctggtt
     8041 gatggccgca gttaaccctg tagaacccgc tgcaaatagt gccagtgtaa tgccgtgttt
     8101 tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg gacgcgtgta
     8161 gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca cgccgtcagg
     8221 atagccgccg aaactgcgga tcaaccagac taataagccc gccagcgcgc cgaacatcag
     8281 ccgaccacga ttggtcgtag aagcagtaac tgggtcagtc aaaataaaga atgcgccgag
     8341 catagtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg gtgagaacaa
     8401 ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa tatgccagcg
     8461 aatcgctttc tgccatagca accacacgcc accagccagc caggcgagat ttacccattg
     8521 ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct gttcaaccga
     8581 atgacctgcg cggacagagg ttttgaatgt atccagcggt gtcgcctgac taatgccatc
     8641 aatacctaag cgtagcgtat tcatatcacc accactggcg gtatgaccgc tgaaaataac
     8701 ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg gtaaccagct
     8761 ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg ccggattaaa
     8821 cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa acaccgtacc
     8881 cagtacgacc atccaccatg gcgctagggg gggaatactt accgccagca ataagcctgt
     8941 cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta gtttgagtac
     9001 gagagcttcg gctaacagag cactgaccga cgccaacagg atctgaacga gagtacccca
     9061 accaaaaaac cgcagttgcg ctgcgattcc tggcacggct gcgagcaaca ccaacagcat
     9121 aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc tgaataccat
     9181 ttagtcctcg tttacaacct tttgctgggc ggcttttttg gcctgaacgc gggcaatagc
     9241 cgcggcaact gcggctttgc gcggatcgac ctgttcttct ggttccgcat tagcctgttg
     9301 ctgttccagc ttgcgtgctt tggcgcgggc aatagcggct tcgacggcag ctttgcgcgg
     9361 atcaacctgt tcttctggct ccgcattggc ctgttgctgt tccagcttgc gtgctttggc
     9421 gcgggcaata gcggcttcga cggcggcttt gcgcggatcg acctgttctt ctggttccgc
     9481 attagcctgt tgctgttcca gcttgcgcgc tttggcacgg gcaatagcgg cttcgacggc
     9541 ggctttgcgc ggatcgacct gttcttctgg ttccgcatta gcctgttgct gttccagctt
     9601 gcgcgctttg gagcgggcga tagctgcttc aacggcagtt ttacgtggtt cagcaacggt
     9661 tgctgcgtcg ttagtttgct ccagttctgc ctgtttcgct ctggcttgcg ctttacgggc
     9721 ttcccgttct gcaataattg cactgttatc cgggcgttcg cccgctttaa tcacaatagg
     9781 ctgtgtagcc tgggcctgtt tctctttcac ccgcgccaga gcggcagcaa tcgcatcttt
     9841 atctttggct gcaggttgaa cggctgcgct cttatgtcgt tcaaggcgag tcgctttttc
     9901 gcgctccaga cgagcctggc gcgcttcgaa acgcgctttg gcttctgcgg cgcgcttttc
     9961 ttcctgacga atagccgcaa tttcagcttt ttcctgacgg aaatattgca ccaagggaat
    10021 attgctcggg caaacccacg cgcaagcccc acattcaatg cagtcggcaa tgttatgcgt
    10081 ggtagcttta tcgtgttgct gacctttgct gaaccagtac aactgttgcg gcaaaagatc
    10141 cacagggcag gcgtcagcac aggcgctaca ccggatgcag ctttgttctt cctgtggttc
    10201 gccaagttca ttggcagagg gagccaacag acagttggta atctttacga ccgggacatc
    10261 cagccatggc aaggtaaagc ccattagcgg gccacccata atcaccattt gatcggcaga
    10321 ggggcagaat ccggcatcat tcaataaatg acgcactggc gtccccagcc gtgcccagac
    10381 gttgcccggg cgagcgattg cttcgccagt cagggttaca acacgctcgg taatcggctc
    10441 gccatcaata acggcacgtt tcactgcata agcagtgccg acgttttgca ttaatacgcc
    10501 gatatcggat gaacgcccgc catgtggaac ctgcttcccg gtcagaatgt aggttaattg
    10561 tttagcaccg ccagaaggat atttggttgg aatcacccgc agagaaatat cgttagagtc
    10621 cgccagcacc gcgcgcagca tggaaatcgc ctgcggtttg ttatcttcaa tgccgataag
    10681 aatttcgcgt ggctgcagaa tatgcgcaag aatgcgaata ccctctacga cctgagccgc
    10741 gcaatcctgc atcaaacggt catcggcggt aatgtacggc tcgcactcag cagcgttgat
    10801 aatcaacgtt tcaatcttat ctccaccacc ctgcaattta acgcctgtcg ggaatccggc
    10861 accgcccagc ccggcaacac caaactgatg tatgcgctcg attaactctt cgcgactgcg
    10921 agagcgataa tcgacccagc cgtcgcgcgg gatccagcag tcttcaccat cggcatcaat
    10981 aatcacgctt aattcagcta aagctgaagg atgagccgta gagtggggcg caatagccgt
    11041 aacggtaccc gaggtgggcg cgtgaacagg cagcattttg ccgcgaccac gggtaagcgg
    11101 ctggccgcgc aatactttat cgccgacgct aacgcacaac tcaccttcag cgccaatatg
    11161 ctgtttcagt ggaataacaa aatgctgcgc cagtagtacc tggcgcaggg gggtaccgtt
    11221 ggtctgggtt ttcatctccg gtggatggat gccgccgttg aaatcccaga ttttattttt
    11281 tctgaatgca gagaataact taagcatggt gttccacggg aatgatacgc acgggaatgg
    11341 tgttcagatc ccatttccag gagtcaggtg tttctgcgac cggttgcaac gagatgcagt
    11401 gcgtcgggca cggatcaaca cataaattgc agcccgtaca gagatcactc attaccgtat
    11461 gcatgacacg ggtagcgcca acgatggcgt ctaccggaca agcctgaata catttagtgc
    11521 agccaataca gttattttca tcaataaccg ccaccatccg cgcaggcgtt agctcttgcg
    11581 cttcgccatc cagcggctgc ggctcgacat taagcaactc ggcaattttt agcatcacag
    11641 cttcgccacc tggggcgcaa cggttgattt tttcaccgtt acagctgatg gcttccgcgt
    11701 aggggcgaca gccgggataa ccgcactgac cacactggct ctgcggtaag atttcgtcaa
    11761 ttttctcaac gaccggatcg tcttccaccg caaaacggcg ggaggcataa cccagaatgg
    11821 cgccaaacgc caggcccagc aggctcacgg cggcaacggc aatccagata gcattcatta
    11881 caacttcacc aaaccactaa agcccataaa ggccagagac ataagacctg cggtaattaa
    11941 cgcaatggca ttaccgcgaa aaggtgccgg gacatcagcc acagcaaggc gttcgcggat
    12001 ggcggcgaag agcaccatca ccagcgagaa accgacagcg gcggaaaaac cgtacagcgc
    12061 cgactgcaag aaattgtgcc cgagattgat attcagcaac gccacgccga gcactgcaca
    12121 gttggtggtg ataagcggca aaaaaatccc cagcagacgg taaagcaccg ggctggtttt
    12181 gcgcaccacc atctcggtga actgcacgac cacagcaatc accagaataa atgccagggt
    12241 gcgcaggtaa atcagattaa gtgggatcaa aatccacgta tcgataagcc aggcgcaaat
    12301 agacgtcagc gtcatcacaa acgttgttgc cagccccatg cccatcgcgg tttccagttt
    12361 tttggaaacc cccataaacg gacagagacc gagaaacttg accagtacaa agttattgac
    12421 cagtacagtt ccgacaaaca gtaacaggta gtcagtcatt tttcggcctg aaataaaaaa
    12481 gccgcctatt atccgttaat ccagagcagg cgacaacagg ttaactgtaa ggttattacg
    12541 gattcacaaa cgtcgctttt acccgcgacg agcgtttgaa ataggggacg atcaatgcgg
    12601 ttgccaacaa agtaaacagc aactggcgaa cggctatgcc aacttccacg ggtgaaaaag
    12661 cgaatgcttt tactgccagc aatacggaaa taagcagcca gataatatag tgtttaggaa
    12721 cacaacgccg acgtttaaag aaagcgatgg tcaaccacag tgtgtaatac cacatggcga
    12781 tagcggtaat aaaagaaacg ccccataaga tttgtgtcgt cagtgcttgt ccgccgagcg
    12841 tttgaaatgt ttgcggagaa gataacgcag cggtatacag caacaacgcc agcgtcgtac
    12901 tcaacagagc gactaaaagc caggccaacg ggccaagcaa ccagcctcca atacgttgtg
    12961 gcgttgtggt ggtcataggt tctccagaat agcgacgcaa acaggcaaat ctaattttag
    13021 gggcgttatt ataaggcttt tataaccgat cgctactttc tttattggac atagtgccag
    13081 acggacttcg gtacctggcc gaggtcaaac aaacgcccgc cagaaaccag ctctgcgcga
    13141 cggtgatcgg cagcacgata catattgatc agttcctgat cgtccgtcag ggtgtaatta
    13201 agatggtcgt agagtttttc cagactttcg agtgaactga ttttgcgaaa ttttaataag
    13261 tagtcctgaa cagtcataaa aggtccataa aataattaat accaaagggg taggtattat
    13321 tagtgatgat aaaatttcac caaaagtgaa cgcttgtgtt attacaataa taagaatgcg
    13381 ctgatggcaa atattcaggg atgaaaaact gctctgaaaa gagtaacgcg ctttactatc
    13441 cctgacaatc accaacaaca tcgaacaaga taataaattc ctggtttaat atccgacaag
    13501 tgaaaacatg cacccggacg ggcagcatgt cgctccacaa gtgcagagct tacttgtgtt
    13561 gtaccgaagc actctgttca ggtggctgat agttgtcaat gtgactcgcc acgccaagaa
    13621 gaatgactga aacgacaaga acgatccaac ctgttaattc aataagacga ttcattacag
    13681 cccacatctc tcttgattga tccattaact tcagggggta aatgttactt agcaataata
    13741 gctccagcaa gatttttact gaggtttttg cgatatttag cttttgtcgt tggaaaattc
    13801 gctatttttt tgacttaagt taaacaacat cccttattgc tggcaggtta ttaaactgtt
    13861 gagcgtgggt aaaggatagt gtcaaatagc catcatactt caatgagagg caatgacatg
    13921 agcgacaaca tccgtgttgg gttgattggg tatggttatg cgagcaaaac cttccatgcg
    13981 cccctgattg cgggcacgcc cgggctggaa ctggcggtaa tctccagcag tgatgaaaca
    14041 aaagtaaaag ccgactggcc aacggttacg gttgtctctg agccgaagca tctgtttaac
    14101 gatcccaaca tagacctgat tgtcattcct acacccaacg atacccattt cccgttagcc
    14161 aaagcggcgc ttgaggcggg taaacatgtg gtcgttgata aaccctttac cgtgacactg
    14221 tcacaagcgc gagagctgga tgcgctggca aaaagcctgg ggcgtgtgct gtctgtattc
    14281 cataaccgcc gttgggatag cgatttcttg acgctaaaag gtttgctcgc ggaaggcgtg
    14341 ctgggtgaag ttgcttactt tgagtctcat tttgaccgct tccgtccgca ggtgcgcgat
    14401 cgttggcgtg aacagggcgg cccaggcagc ggtatctggt acgatttagc accgcatctt
    14461 cttgatcagg ccattacgct atttggttta ccggtcagca tgacggtaga tttggcacag
    14521 ttacggcccg gagcgcagtc gaccgattat ttccacgcca tcttgtccta tccacagcgg
    14581 cgagtcattt tacacggtac catgctggca gccgctgagt cagcacggta tatcgtgcat
    14641 ggatcccgag gcagttatgt gaaatatggc ctcgatccac aggaagaacg tctgaaaaat
    14701 ggcgagcgtc tgccgcagga agactggggc tacgatatgc gtgatggcgt acttacccgc
    14761 gtggaaggtg aggaacgtgt cgaagaaacg ctgttgacgg tgcctgggaa ttatccggct
    14821 tactatgcgg cgattcgtga tgcgttaaat ggcgatggtg aaaatccggt tccggcaagc
    14881 caggcaatcc aggtaatgga gttgattgag ctgggcatcg aatccgccaa acatcgcgcg
    14941 acgttgtgcc ttgcgtgaaa aatggatggt gcaatttgca ccatccattt ttgttacttc
    15001 gcggcgactt tttctcgcag tgcgcgtttt tcctctgcgc tgaggaaagc catttccaga
    15061 ccattaatct gtgcctggcg gatttgctca cgggataacc cagcagctgg cgcggcaacg
    15121 gtatattcgt gaatgatatc cactccctgt acgccgggat cgtcagtgtt aatgctggca
    15181 cgaatgccat gctcaaggaa cgttttcagc ggatgtgcag ccagctctgc tacggtgctg
    15241 gtctgaatat tggaggtcag acaggattca ataccaattt gttgctcggc gagaaaatcc
    15301 atcagcgccc gatcttcaat ggcttttacg ccatgtccaa tacgctccgc acccagttca
    15361 cgaatcgcct gccagatgct ttccggcccg gcagcttcgc ctgcatggac ggtaatatgc
    15421 cagcccgcat cacgcgcgcg gttgaagtga gaaaggaaca gacttcccgg gaatccaagt
    15481 tcatcaccag ctaaatcaag tgcggtaatc tggtcacggt gggctaaaaa ggcctccagc
    15541 tcttgctgac aggcggcttc gccgaaggtt cggctcataa tgccgataag cttcgcctgc
    15601 acaccaaagg tgcggcaacc ttcacgtacg ccatcgatca ccgcttcgac aacacccgct
    15661 acaggcagct tatgtgccat tgccatgtag cctggtgaaa aacgcagctc gacatagcgc
    15721 aggccgttac gggctgcatc ttcaatgttt tcaaatgcca cgcggcggca ggcatcaaga
    15781 gaggcgagaa ctttaacgcc ccagtcaagt ttggtcagaa agctcaccag atcgggttcg
    15841 ttggcaatga cctgaacgtg gggaatcagt gtttcaaggg attgtgcagg aagcgagata
    15901 ttatactggc ggccaagttc aagaatggtc tggggacgaa tgttgccatc aaggtggcga
    15961 tggatatcag ttaatggcag ggtggtatca atcatggtcg cactcttttt ctggatataa
    16021 aagtgcgcac cattataaaa acaaagcagg gtaaaaatcc acgattgcgc aacgattttt
    16081 tacgttgcgc aattggggtt agcgaacagc gcggatggcg tttattaatc cagccacacc
    16141 tttttccagt ttcgaacgtg ggcagccggc attgagacgg acaaaaccac gaccttcttc
    16201 accataggta taccccggca tgatcgcgac tttttcttgc tcgataagcg ctttttgcaa
    16261 cgcgttgtcg tcaatattca acggacgtag atcaagccag gccagataag tggattgcgg
    16321 gatctgccag ttgagttcag gaaacgcggc gttcatttta tctgagatat acgtcaggtt
    16381 atctttcaga tagacgcgta aggcatccag ccacggtgca ccttgctgat aggcggcgat
    16441 atgggcagtt aacgccagta ccgaagggga agaaagccca tcacgtcctt tcagtgccga
    16501 taaataggca tcacggctgc tgctattttc tataatcccg taagcaccgg tcagcgcggg
    16561 aatattgaaa cttttcgatc ccgatgtcag caacgcccag tctccccgcg ccacattact
    16621 ccagggaata tgcggctgct cgccccagac catatccata tggatttcat cggaaataac
    16681 ccgcacatca tgacgctcgc acaggtcagc catgatctcc agctcatcgc acgtccacac
    16741 tttcccggta ggattctgtg ggttacacag gagcataatt ttacattctg gtttcgccaa
    16801 aacggcttcc agcttgccca tatcgcaaaa ccagccgtca gcctgcttct ctaaagcaac
    16861 gggcattact gtgcgctggt taccttcaat ggccttgtaa aatgcgtcat aggcgggcgt
    16921 gtggatcacc acgccttcac ctgtttcaga ccactgacga atcagttctg aaaccatata
    16981 gatgacagaa gggccataca ccaccgtctg cgtatcaatt gcggtgtaat gctgggtgga
    17041 aaaccagtgg gcaatagccg cgagaaactc atcgtttttc cagcggctgt agccaaatac
    17101 gccgtgcatc aggcgctgat tcagcgcctc gataatgcag ggggcagtgg caaaatccat
    17161 gtctgaaatc gtgaacggta acaggtcagc agtgccgaaa cggtcagcga cataatccca
    17221 ctgtgtacac catgtgccat gacgatccac gacctttgaa aaatcgaaca tatcttatcc
    17281 ttatgcctgg acagtatgca tcagaccggc catttcatct tttactgact gaacttgtgg
    17341 cccgataaca acctgcaggt tatgttgatt aagttgtact acgccaattg cccgattgtc
    17401 cttcagtgcc tgcacattaa caagcgacat atcttccaca gacaaacgca gacgggtaat
    17461 gcagttatcg aggctgacaa tattgtcggc accgcctaat gcttcgagga ttgcaggaac
    17521 gttgtaacct gatttacccg gcgcaccggc aacggctttt tcgattgagc tggcaacttc
    17581 gctatcgcgc cccggggttt tcagattgaa gcgggtgata gcgaaacgga agatgacgta
    17641 gtaaacgaca aaccagattg ccgccaccac tggcaccatg taccacttgg ttgacagacc
    17701 atgcaaaata ccgaacacca cgaagtcgat gatattgccg tcggtattac cgatggtgac
    17761 gccgagcaca gacatgacgg tgaagccgag gccggttaac agcgcgtgga tgacatacag
    17821 aactggcgct acgaacagga acaggaattc cagcggttcg gtagtgccgc caacgacgca
    17881 ggcgatcagg ccagaaatca gcagaccttt aattttatgg cgattttccg ggcgcgcgca
    17941 gtgatacata gctaacgctg cacctggtag accgccgaga aacgcaggca ttttaccttg
    18001 cgaaaggaaa cgcgtgacgc tttcagaaaa accgtgagtg gtcgggcaac tcaattgcgc
    18061 ctggaagatg gtcagtgcgc cgctgacggt ttgaccgcag acctcctgcg tgccgcctgc
    18121 gtcggtaaag cgaattaatg ccaccagaat gtgatgcaga ccaaacggca acagcagacg
    18181 ttcaccggta ccaaacagca tcggtccgaa atcacccgcg ctgtttatca tatggcccaa
    18241 gccgctaata cccatggcga aaatcggcca gactaatgga atcaccaggc cgacaaggcc
    18301 catcaccagc gaggagataa ttggtacgaa gcgcgtaccg ccgaagaatg ccagcgcatc
    18361 cggcaggcgg atattatgga aacgctcatg cagcatccag acgataatac cggcgatcac
    18421 cgcaccgagg atcccggtat cgatcgactg gatcccaaga atgctctgga tgttattggc
    18481 tttcagaacg gcggcatctg tggttggcag aatgccttta ttggtcaacc agaagtttac
    18541 cgcgaggttc attaccgcat aaccgacgaa gccagcgaat gccgctacgc ctttattttc
    18601 gcgtgccagg cccagcggga tggcgataca gaacatgaca ggcaggaaac taaaagcaaa
    18661 cgagccaatc ttactcatcc aggtaaagat agcctgcaac acggggttgc ccaggaccgg
    18721 gatcagggtt atgacatcat ggctgctaag agaactacca atgccgagca taatgccgca
    18781 gaacgacaat aatgccacgg gtaacatgaa ggttttgcct aactgctgga agaactccca
    18841 cagcgtgact ttcggtgctg ttttcgccgt cataaaacga ctcctcgtag agaacaatct
    18901 ggattcagta aattgcgcga gaagataaaa cgttttatca aattttagtg agtcataaat
    18961 cacattacgc aacgataata gcgggtataa gataaataaa aggtaaaacg ttttatctgt
    19021 cacataatca gggagtaggt catctgcatg gctaccgcca aaaaaataac cattcatgat
    19081 gttgcgctgg ctgcgggcgt gtcggtaagt accgtttcgc tggtgcttaa tggcaaaggg
    19141 cgaatctcta ccgccacagg agaacgcgtt aacgccgcca ttgaagagct gggatttgtg
    19201 cgcaatcgcc aggcgtcggc gctgcgcggc gggcaaagcg gcgtcattgg tttgatcgtc
    19261 cgtgatttat ctgcgccgtt ttacgccgaa ttgacggccg gattgacgga agctctggaa
    19321 gcacagggac ggatggtttt tttgcttcac ggcggtaaag acggcgagca gctggcacag
    19381 cggttttcac tgttactgaa tcagggtgtc gatggtgtgg taattgccgg ggctgcagga
    19441 agcagcgatg acctgcggcg gatggcagaa gaaaaagcta tcccggtgat tttcgcttcc
    19501 cgtgccagtt atcttgatga tgttgatacg gttcgcccgg acaacatgca ggctgcacag
    19561 ttgttgacgg agcatctcat tcgcaatggg catcagcgga tcgcctggct gggagggcaa
    19621 agttcctcat taacccgtgc agaacgggtg gggggctatt gtgcaactct actaaaattt
    19681 ggcctgccgt ttcacagcga ttgggtgttg gagtgcactt ccagccagaa gcaagccgcg
    19741 gaagctatca cggcgctttt acgtcataac ccgaccatca gtgccgtggt ttgctataac
    19801 gaaactattg cgatgggggc atggtttggt ttgctgaaag cagggcggca aagcggggaa
    19861 agcggagtcg atcgttactt tgagcaacag gtttcgctgg cggcatttac cgatgcgaca
    19921 ccaaccacac ttgatgatat acctgttacc tgggccagca cgccagcgcg ggaacttggt
    19981 atcacacttg cggatcgcat gatgcaaaaa atcacccatg aagagacgca ttcacgcaat
    20041 cttattattc ccgcccggct cattgcggcg aaataatcct ctctttatct gctatacttg
    20101 gtagtgtcct ttcctcaagg ttaatgataa aaaatgtggc acacaggcgg tgccttttat
    20161 ttttactgaa gttcacagga ggtttatgtg tttaattctg acaacctgag actcgacgga
    20221 aaatgcgcca tcatcacagg tgcgggtgca ggtattggta aagaaatcgc cattacattc
    20281 gcgacagctg gcgcatctgt ggtggtcagt gatattaacg ccgacgcagc taaccatgtt
    20341 gtagacgaaa ttcaacaact ggatggtcag gcatttgcct ggcgttgtga tattacttcc
    20401 gaacaggaac tctctgcact ggcagacttt gccgtcagta atctgggtaa agttgatatc
    20461 ctggttaaca acgccggtgg cggtggtcct aaaccgtttg atatgccaat ggcagatttt
    20521 cgccgtgctt atgaactgaa tgtgttttct tttttccatc tgtcacaact tgttgcgcca
    20581 gaaatggaaa aaaatggagg tggcgttatt ctgaccatca cttctatggc ggcagaaaat
    20641 aaaaatataa acatgacttc ctatgcatca tctaaagctg cggccagtca tctggtcaga
    20701 aatatggcat ttgacctggg tgaaaaaaat attcgggtga atagcattgc gccgggggca
    20761 atattaaccg atgccctgaa atccgttatt acacca
//
LOCUS       DAJTRD010000068        20777 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTRD010000068 DAJTRD010000000
VERSION     DAJTRD010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20777
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.068"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..579
                     /locus_tag="OO315_002738"
     CDS             <1..579
                     /locus_tag="OO315_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5891591.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OO315_002739"
     CDS             597..1235
                     /locus_tag="OO315_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5891592.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OO315_002740"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OO315_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5891593.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO315_002741"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO315_002741"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5891594.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OO315_002742"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OO315_002742"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5891595.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO315_002743"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO315_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5891596.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OO315_002744"
     CDS             complement(5552..6613)
                     /locus_tag="OO315_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5891597.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OO315_002745"
     CDS             complement(6610..8007)
                     /locus_tag="OO315_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5891598.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNAAFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OO315_002746"
     CDS             8163..9161
                     /locus_tag="OO315_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5891599.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO315_002747"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO315_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="OO315_002748"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="OO315_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO315_002749"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO315_002749"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5891600.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO315_002750"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO315_002750"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5891601.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="OO315_002751"
     CDS             complement(14232..14855)
                     /locus_tag="OO315_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5891602.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="OO315_002752"
     CDS             complement(14852..15274)
                     /locus_tag="OO315_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5891603.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16071)
                     /locus_tag="OO315_002753"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="OO315_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="OO315_002754"
     CDS             complement(16089..17141)
                     /locus_tag="OO315_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5891604.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="OO315_002755"
     CDS             complement(17172..18014)
                     /locus_tag="OO315_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5891605.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEATMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="OO315_002756"
     CDS             complement(18001..18882)
                     /locus_tag="OO315_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5891606.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="OO315_002757"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="OO315_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20777)
                     /gene="ycjM"
                     /locus_tag="OO315_002758"
     CDS             complement(20200..>20777)
                     /gene="ycjM"
                     /locus_tag="OO315_002758"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5891607.1"
                     /translation="NWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAIL
                     LSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAVY
                     HELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHVN
                     ITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5070 a         5666 c         5053 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gccgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgtc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagttta
//
LOCUS       DAJTRD010000069        20709 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTRD010000069 DAJTRD010000000
VERSION     DAJTRD010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20709
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.069"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(77..4537)
                     /gene="mukB"
                     /locus_tag="OO315_002759"
     CDS             complement(77..4537)
                     /gene="mukB"
                     /locus_tag="OO315_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5891608.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="OO315_002760"
     CDS             complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="OO315_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5891609.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="OO315_002761"
     CDS             complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="OO315_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5891610.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="OO315_002762"
     CDS             complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="OO315_002762"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5891611.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7462..8241
                     /gene="elyC"
                     /locus_tag="OO315_002763"
     CDS             7462..8241
                     /gene="elyC"
                     /locus_tag="OO315_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5891612.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8218..9111)
                     /locus_tag="OO315_002764"
     CDS             complement(8218..9111)
                     /locus_tag="OO315_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5891613.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="OO315_002765"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="OO315_002765"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5891614.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="OO315_002766"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="OO315_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5891615.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /locus_tag="OO315_002767"
     CDS             complement(10242..11474)
                     /locus_tag="OO315_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5891616.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="OO315_002768"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="OO315_002768"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5891617.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="OO315_002769"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="OO315_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5891618.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="OO315_002770"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="OO315_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="OO315_002771"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="OO315_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5891619.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="OO315_002772"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="OO315_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5891620.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="OO315_002773"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="OO315_002773"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5891621.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="OO315_002774"
     CDS             complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="OO315_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5891622.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4982 a         5727 c         5029 g         4971 t
ORIGIN      
        1 ccgttatgtg ccattttttt agtgcagaaa cggagttttc ggaaaaagaa aaggcggcat
       61 tgctgccgcc ttaattttaa ctcgcctggg aaggcgcttc gtctcttcct agaagcgttt
      121 cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacatgttcg gtattctgga
      181 agactttacg taccagttta taggtggtgc ctttttccgg actgatattt tccggcgctg
      241 cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg atagaacgag
      301 catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata tctttaccgc
      361 gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc gacataccgg
      421 taccaatcgc ctcaccggtc gacaatgcac cagactctgc gcgcaaccaa ccatcggaac
      481 cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt tcttcgccaa
      541 tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga tacaatttcg
      601 ccaacgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc tgctcgtgct
      661 gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg ttgagacgca
      721 cgctgttcac ctgaccaaac gatacgttct gcaacccctg gttgagcata cggatacggt
      781 tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac gctgcgggaa ctgatcgcca
      841 gtttctgttc acgggaggtt aattcttcgg tcagacggct aagttcaatc tccatctgtt
      901 cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt tcacgcagat
      961 gctgataaac cgccacgaag aactgaattt tacgctccgg acgtttcgga tcttccgaca
     1021 tgcgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc gcacctaacg
     1081 ccttatccga catggaacgc aaatcatcag cggagagata agccagctca cgacggtgta
     1141 agcggcgctc aacgccgtta tctttcacca tgcgcatcac cgcacaccag cccgctttgg
     1201 cggtcactac ctgctcgcgc atctcaaagt aatcccgctc cagcttgcgc agtttgcggg
     1261 tcaggttgtc catctccgct tcgcagaagg taagcgcttt ttccagttga ttgcggcgtg
     1321 aacggttatt gctcagttgc gcatgcagct cgtcacggcg aatacgcgcc cgctcttctg
     1381 ccccgctatc agcacgcacg ccgatatcct gcaattcacg ttgcagatcg ttgagtagct
     1441 cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac tgactcaact
     1501 gcgctgcgtg accgcgcaac gcttcgcgag cgcgggtacg ctccgcttcc gcctgttcca
     1561 gacgttcacg cagtttttcg ttgagatcgc tgttaccgct aagcatttct gccgagtcag
     1621 aatagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgct tgctgacggg
     1681 catcgcgctg catctgctga gagtacgcgt aatcttcttt taactgttcg aactgttccg
     1741 ggtcgctctg caataccgaa acgatcggtt ccagtttcgc cagttgattg ccaaactgct
     1801 gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca tcgacgcgat
     1861 ccgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc agcgccgtaa
     1921 cgccttcttt cgcctgctca aactgaatac gctgctgctg gttatcattt tcatgattac
     1981 tcaacgcccg ctccagttcg actcgacggc tgttcagttg acggatttct gcttccgggt
     2041 cagactcaaa cgccaccgcc agatgactgc cgataaagcg gctgaacgcc tgatgcagac
     2101 gctgggtttt ctgtacatca aaggagagcg tggcgaagcg ttcggaaagc acttcacgct
     2161 cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc ggcacttccg
     2221 ggaaacgtga ataacgccac tgtcgatcgg cgattttcac caccaccgct ttttccagct
     2281 catcaacgct gaacacgcta tcatcgaatg actgcggatc cccttcgatc aaatagagat
     2341 cttccgggca atcggtcaag ccttccaggt gttcggttac ctgtgacaga tctggcacca
     2401 cgatggcgtg gcgtgacggg ccatacagag ccgagaagta cggcgcatct tccaggctaa
     2461 cgtcgtcata aatttctgac agcagcacac caccaaaacg ctccgccagc gcgttcagac
     2521 gctgatcttc agagccgcca ggctggctta aacgttcgat ctcttcatcg acggcgtttt
     2581 tgcgcgcgcc tacttcatcg cgttcaacaa tcgcctcgcg ctcacgctcc agcaactgtt
     2641 gcagatattc agtgacgtcc tggctggagg agaactcttc gccgcactgt tcactcaact
     2701 ggttgagact gttttgcgct gccagccaaa ccggtgcacg ctgcatcaaa ctctgaatgc
     2761 gagactgcag ctgttcctgt tcctgccgca gtgccatccg ctcttcacgg gcgttagata
     2821 cgctatcgga aagagaggca atgcgtgctt ccagctcctg atgcagagct tccagttcat
     2881 caatatcaaa attcttgccc tgacgtttgc agaaatctgc cagcagacgc tcagcttctt
     2941 gctgctcacg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggc tgaacctgct
     3001 ctgccaggtg acgctgatca accccttcgc gcaatagttc gcgagcgaca tcccacgcct
     3061 cgttacgcgc cagcgggccg ttgattgcca ccaccagctg ataagcctgc tcaaactggc
     3121 tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt tcagtcgctt
     3181 ccagctcttt cgcctggaag gtttccagcc attcggcggc gctgtcggcg gttaagtccg
     3241 gcagatggca cagttctttg gcacgattaa gcgcagcaat cgcctggtta tactggatcg
     3301 cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc aagctggctt ttcagctcgt
     3361 ccacttccag ttcggcagct tccgcgcgag cctcattctc ttcctggcgt tcgatggctt
     3421 ctgccaccac ttcattttgc tcttccagac ggatctgcag ctcatcgaga tccacttcgt
     3481 agcgttcgat cttctcctgc tgacgcaatg cggtttgcac caggttcagg tgatcgctgg
     3541 ccgcctgata atccgcttcc agatcacctt cggcaccgtt gtgctctgcc agctcacgcg
     3601 ccatatcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagta tgtagctcgc
     3661 gacgaaactc cagggctttg tcgagatgga cacggcgctc gttggcgtga cgcatgtagt
     3721 ccgccgccac gtagttggta gcttcgctga tcagatgctt aaacaggtca cggtccgact
     3781 gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg cagcgccgct tccatgtcct
     3841 ggaacgcttt acgcacgccg ctgttttccg gcaacaggta gtcgcgcaga gaacgggtaa
     3901 tggcactgga gatcccgcca tacagcgaag cttcgatcag acgatagaat ttgctacggt
     3961 cagatgcgga gcgcagacga cgcgcgatga tgcccagatc gaacatcagc gagtggtaat
     4021 cggtaatgga gttgaactgt ttaaactgca cgccttccat cgcctcgagc ttgtctttca
     4081 gctcgttaag cggcagcacg cgcgcctggc gttcgttcag ggtttcggtc accagctgtg
     4141 tcggctgcac cgacatcggc agtccctgaa tggcaaatgg tttgatatcc actttacgat
     4201 cgcgtccggc aacctgttgc agacgcacac cgaccaccac gcgctggtgg cgcgagttaa
     4261 tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga cctttatcgc
     4321 gcgaaccgct ggtggccccg gcttccgtag tgttacggaa atgcagcagg gtcaggtcgg
     4381 ggatcagcgc cgtaacgaac gccgccatcg tggtggattt acccgccccg ttaccgccgg
     4441 aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc aaaaaagccg ttccagttaa
     4501 tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc cgctatctgg
     4561 ctgactctct tcggtttcat cgttgagttg cagatgattt tcaatcggca ttgcttcgcc
     4621 atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca catcggctcc
     4681 gaagcggaac accgattcgg taatgcggaa cttgctgctg tcgtggccca taaaccacac
     4741 catgcctaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca acttctgacg
     4801 gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagtttcg cttcatcggc
     4861 cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa atcccctcat tcgccagccg
     4921 ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca gttccgacaa
     4981 gacggaacgc gggataagag tggtggaacg tgggcgtaaa tagaagaacc cttccggtgc
     5041 gcgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct gaaaatccat
     5101 caggaatgca tgattatcca gttcgtcgag gccaatatgg cggcctgaac gtaaggcgct
     5161 gtccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg gcatcacttg
     5221 ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta atcggctgcc
     5281 atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc gcctgatcaa
     5341 taacaatacg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga tattcgcgta
     5401 ccaccagacc aagatccagc ggcacttgtc tggttttgta cacggcaagt tgttcttcga
     5461 tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtattccaga tcctcaggaa
     5521 gttccccagt cacttcttca tcgcgcagtg ccatctcttc gtcacgcata tccagcagac
     5581 gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt accgactgac
     5641 gtaaccgctg agcaaagacg cggtttttat ccatatcgat cgcggtacga ataaatttgt
     5701 gtacgtggcg gtcgtagcca atccacaagt cgatggattg ctggccccaa ctgataatac
     5761 gatcgagttt gctctgcaga tcgaacacca gacgatcgac gaaatgcaga tcgtcatggg
     5821 tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttgtcgcct gccgcttcca
     5881 gcgtatcctg caattcacgc agcgttccgg aagtttccga aagcaacaat tcacagctgg
     5941 aaatcgccgc ccgccagtct ttgttcagca actgggcgat atcgtccttc acctgctgct
     6001 gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct gctaccgaat
     6061 atttcagtgg cgcatagaca ttacggtgcc agtgaaattc atcaccgccc tcttcggcgg
     6121 catctgctgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa agacgcagcg
     6181 tagaaaactc gcgctgacgg atgtagtagt cagtaatgcc gatgccgagc ggcgtcagac
     6241 ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga cgttgacgca
     6301 ccatgtcgtt gatcgcgtta ttggcgcgca cgccgatggt ttcgctggtt tgctcaaacg
     6361 catcactcac atggcggaat gcatccacca gctcgccttc actcatctca ccatccagac
     6421 gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc ggcagcgaga
     6481 tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg gaaaattcac
     6541 tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac gccctagggt
     6601 aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt catagcagtc
     6661 gcgctgctgg tacttctcgc gcagataatc atgaaacacg cgaacgcctg tcttacccat
     6721 aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc gtggataatc
     6781 tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat caaaattccc
     6841 ggcgaccatg ttatgcatca acaaaccatg cgcattgtag aacattaacg acaacactcc
     6901 gcctggacgc aacactgacc acagggtctg caatacactg cggggatcgg ccacccactc
     6961 gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgcgaag caacatcctg
     7021 agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg ccgcctgttt
     7081 tgcgcggtcg atcatctgcg cagaaagatc gcataaaata acctgatgcc cgcgctcggc
     7141 cattttgatt gcggtctgcc cttctccgcc gccagcatcc agcacacgca gtttttgcgg
     7201 gcccatttcc gccagcacgc gatcgagatc ctgccacaga atagcctgtc gaagctgccc
     7261 tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcaa aattgcgatc
     7321 ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc tattttgtca
     7381 cagccacggc gagaatgaac ccgtctggtg taatatcgct agcgatccct tgctatatgg
     7441 ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg caatatgttg
     7501 ctgccacttc cgctgatgtt gttaattatt ggcgctggcc tggcgctgct gtggtttagc
     7561 cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcact cttgttattg
     7621 agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta tccgacatgg
     7681 aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac ctggaacccg
     7741 cagtgggcac cgagctctaa tttaatcaat aacagtctgc cacgcctgaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg cgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatccgc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcagtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa cagcgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttgc ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatcg ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcca ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tggcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttaccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cggagctgat agccatattg gtatcaatac
    20521 cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt cgcaatcgcc agcagtaatt
    20581 tagtattctt catgtgaatc atccgttgat taatcatata taaatattat gtatcaacaa
    20641 cttacattga caagcgacgc taccattaat caaaatgact cacaaggtcc gaaaaaaaac
    20701 gccgacaat
//
LOCUS       DAJTRD010000070        20691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTRD010000070 DAJTRD010000000
VERSION     DAJTRD010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.070"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            81..350
                     /locus_tag="OO315_002775"
     CDS             81..350
                     /locus_tag="OO315_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5891623.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            344..709
                     /locus_tag="OO315_002776"
     CDS             344..709
                     /locus_tag="OO315_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5891624.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(710..1498)
                     /gene="bioH"
                     /locus_tag="OO315_002777"
     CDS             complement(710..1498)
                     /gene="bioH"
                     /locus_tag="OO315_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5891625.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1536..2219
                     /gene="gntX"
                     /locus_tag="OO315_002778"
     CDS             1536..2219
                     /gene="gntX"
                     /locus_tag="OO315_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5891626.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2278..2853
                     /gene="nfuA"
                     /locus_tag="OO315_002779"
     CDS             2278..2853
                     /gene="nfuA"
                     /locus_tag="OO315_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5891627.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3214..4530
                     /gene="gntT"
                     /locus_tag="OO315_002780"
     CDS             3214..4530
                     /gene="gntT"
                     /locus_tag="OO315_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5891628.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="OO315_002781"
     CDS             complement(4575..6659)
                     /gene="malQ"
                     /locus_tag="OO315_002781"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5891629.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TTTKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6669..9062)
                     /gene="malP"
                     /locus_tag="OO315_002782"
     CDS             complement(6669..9062)
                     /gene="malP"
                     /locus_tag="OO315_002782"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5891630.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEVPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9674..12379
                     /gene="malT"
                     /locus_tag="OO315_002783"
                     /pseudo
     CDS             9674..12379
                     /gene="malT"
                     /locus_tag="OO315_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="OO315_002784"
     CDS             complement(12422..13438)
                     /gene="rtcA"
                     /locus_tag="OO315_002784"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5891631.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="OO315_002785"
     CDS             complement(13442..14668)
                     /gene="rtcB"
                     /locus_tag="OO315_002785"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5891632.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14849..16447
                     /gene="rtcR"
                     /locus_tag="OO315_002786"
     CDS             14849..16447
                     /gene="rtcR"
                     /locus_tag="OO315_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5891633.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16429..17187)
                     /locus_tag="OO315_002787"
     CDS             complement(16429..17187)
                     /locus_tag="OO315_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5891634.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="OO315_002788"
     CDS             complement(17204..18034)
                     /gene="glpG"
                     /locus_tag="OO315_002788"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5891635.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="OO315_002789"
     CDS             complement(18079..18405)
                     /gene="glpE"
                     /locus_tag="OO315_002789"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5891636.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18595..20100
                     /gene="glpD"
                     /locus_tag="OO315_002790"
     CDS             18595..20100
                     /gene="glpD"
                     /locus_tag="OO315_002790"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5891637.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20369..20599)
                     /locus_tag="OO315_002791"
                     /pseudo
     CDS             complement(20369..20599)
                     /locus_tag="OO315_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4893 a         5693 c         5213 g         4892 t
ORIGIN      
        1 gtcagtagat actctaccct gcttaagtgc tactttgtgc tatcttgcat gtaacacaaa
       61 gtagcactta gggaactgat atgcagacag tgaaattacg gcaacaagga ggagccatga
      121 tcgttactat cccacgagat cttgccatcg atcttggctg gtcaccgggc acagagctta
      181 ccgtggagaa aaaaggcgat agcgttaatt tgcgcgccac agagcacaaa ccgcgtgggc
      241 gtcttaccgt cgcgcagttg ttgagccaaa tcgatgaaag cgaaattacg gagctaaatc
      301 agtccacaga aggatgggct gaagggaaaa aaggtaatga agcatggtaa aggcacggac
      361 gccacatcgt ggtgagatct ggtattttaa ccctgatccg gttgccgggc atgaacttca
      421 ggggccacat tattgcattg tggtaacgga caaaaaactc aacaatgttt taaaagttgc
      481 tatgtgctgc ccgatttcaa caggggcaaa tgcagcacgt tccacagggg tgacggtgaa
      541 cgtcctcccc cgtgatacgc aaaccggtaa cctgcatggc gttgtacttt gtcaccagct
      601 aaaagccgtc gatcttattg cccgtggcgc taaatttcat accgttgccg atgaaaaatt
      661 gattagtgaa gttatcagta aactggtgaa tttaatcgac ccacaataat tagctctcac
      721 ttaccaccaa caccctctgc ttcaacgcca ccagcaggtg acaaaactcg accggatgcg
      781 aaataaacgg cgcatgggcc gctttggcga agatatatga ttcgctgtga ggccaaagtt
      841 tatccagcat cggcaccact ttgcgcggca ccagaccgtc gagatagcca tacaatcgca
      901 aaaacggcat ggacacgttt tgcagcggct gacggagatc gaccgttttc aggatttcca
      961 gcccgccatt aagcacgtca acctccggca tcggtaacgc cagaacggtt ttcttcaacg
     1021 cccgcgcatc ctggcgcgcc gtttcagtcc ccatggtttg taacgccagg aaccgctcca
     1081 ctgtacgctg aaaatcatca ctgagttgct gctgaaatcc cgccagcacg tccggtttta
     1141 tccccggcca ctcgtcacga gcactaaaac aaggtgacga cgccacggtg accagcgcct
     1201 gaacacgctc gggatgggtt aacgcaatct ggcttgccac cagcccgccc agactccagc
     1261 ctaaccaaat ggctttatca ggtgcctgtt gcagcacggc ttcggccata tcagcaagtg
     1321 acagcgcacc aaatccccgg ctacgcccga agccgggcag gtcaacaagg tgcagcgtaa
     1381 aatgcgagct aagttcctcg tcaatgcaac gccacacttc ggcattcagt ccccatccgt
     1441 gcagcagcac aagatgaaca ttcccctgac ctttggtctg ccaccagatg ttattcatcc
     1501 gctattgttc tcttttgact tacaaggatg aacatatgct aacagtaccg ggattatgct
     1561 ggctatgccg aatgccactg gcgttaggtc attgggggat ttgttcggtc tgttcacgcg
     1621 ccacccgcac agataaaacg ttatgcccac aatgtggatt acccgccaca cactcccatc
     1681 ttccctgcgg tcgctgcctg caaaaaccgc cgccctggca aagactggtc acggttgccg
     1741 actatgcgcc gccgttaagt ccgcttatcc accagcttaa attttcccgg cgcagtgaaa
     1801 tcgccagcgc cctgtcacgt ctgttactat tggaagtctt acacgctcgt cgcaccaccg
     1861 ggttgcaatt gccggatcgc atcatcagcg ttccgttatg gcagcggcgt cactggcgtc
     1921 ggggatttaa tcagagcgat ttgctgtgtc agccgttatc acgctggttg cactgccaat
     1981 gggatagcga agccgtcaca cgtacacggg ccactgcgac ccagcatttt ctcagtgccc
     2041 ggctgcgcaa gcgcaacctg aaaaatgcct ttcgtcttga attgcccgtg caaggtcgcc
     2101 atatggtgat tgtggatgat gtcgttacca ccggaagtac cgtcgcagag attgcgcagt
     2161 tgcttttacg caatggtgcg gcgactgtcc aggtctggtg cctttgtcga accttgtaga
     2221 gcctcgatga tgggcgtatt ataaccaact aaaatagtca actattaggc cattactatg
     2281 atccgtattt ccgatgctgc acaagcgcac tttgccaaac tgctggcaaa tcaggaagaa
     2341 gggacacaaa tccgcgtatt tgtgattaac cctggcacgc ctaacgctga atgtggtgtt
     2401 tcttattgtc cgccggatgc cgtggaagcc accgacactg ccctgaaatt tgacctgctg
     2461 accgcgtatg ttgatgagtt aagcgcacca tacctggaag atgcagagat cgactttgtt
     2521 accgaccagt tgggttctca gttaacgctg aaagccccga acgccaaaat gcgtaaagtg
     2581 gcagacgatg caccgctgat ggagcgcgtg gagtatatgc tgcagtcgca gatcaaccca
     2641 cagcttgctg gtcacggtgg tcgcgtttcg ctgatggaaa tcaccgaaga cggttacgcc
     2701 attctgcaat ttggcggcgg ctgtaacggt tgttccatgg tcgatgtgac gctgaaagaa
     2761 gggatcgaga agcagctgct gaacgaattc ccggagctga aaggtgtgcg cgatctcacc
     2821 gaacaccagc gcggcgaaca ctcctactac taagttatcc cctcttttgt agattgcccg
     2881 atgcgacgct aaagcgtcat ttcgggccta cgttgaacat gtgccagacg ttggcacagc
     2941 attgctttgg caaatccctc ccttcgtttc cccgcgataa tatgaccaac ctctcataat
     3001 ttaaatttac cccgctctgg tgattctcaa acgccaaatg ttacccgcat cattcacatg
     3061 ggtaccaaac atactcctga catctgacta caataattag ttttagtggg tatcagtcgt
     3121 ggtgccgcaa tatctctgtt cccgattggg ataattagag tttgtcgtca gaaaattgac
     3181 gttacccata acaaatgaaa ggccaggtaa atcatgccat tagtcattgt tgctatcggt
     3241 gtaatcttgt tgttgctcct gatgatccgc ttcaaaatga acggcttcat cgctctcgtc
     3301 ctcgtggcgc ttgctgttgg attaatgcaa ggaatgccgc tggataaagt tattggctcc
     3361 atcaaagccg gtgtcggcgg gacgctcggt agccttgccc tgatcatggg ttttggcgca
     3421 atgctgggca aaatgctggc agactgcggt ggcgcacaac gtatcgccac cacgctgatt
     3481 gccaaatttg gtaaaaagca catccagtgg gcggtggtgt taaccggttt taccgttggt
     3541 tttgccctgt tctatgaagt gggcttcgtg ctgatgctgc cgctggtgtt taccatcgcg
     3601 gcttccgcga atattccatt gctgtatgtt ggtgtaccaa tggcggctgc actgtctgtg
     3661 acccacggct tcctgccacc gcatccgggc ccgactgcga ttgccaccat tttcaatgcc
     3721 gatatgggta aaaccctgct gtacggtact attctggcaa tcccgaccgt gattctcgcc
     3781 ggtccggttt acgctcgcgt gttgaaaggt atcgataagc caatcccgga aggtctctac
     3841 agcgcgaaaa ccttcagcga agaagagatg ccgagctttg gcgtcagcgt ctggacctct
     3901 ctggtgccgg ttgtgctgat ggcgatgcgt gcgattgccg aaatgatcct gccgaaaggt
     3961 cacgctttcc tgccggtagc ggagttcctc ggtgacccgg taatggcaac gctgattgcc
     4021 gtgctgattg cgatgttcac ctttggtctg aaccgtggtc gttcaatgga tcagattaac
     4081 gacacgctgg tttcttccat caaaatcatt gcgatgatgc tgttgatcat cggtggtggc
     4141 ggtgcgttca agcaggtgct ggtagacagc ggcgtggaca aatacattgc ttccatgatg
     4201 cacgaaacca acatttctcc gctgctgatg gcctggtcga ttgctgccgt actgcgtatc
     4261 gcgctgggtt ccgcaaccgt tgcggcaatc actgcgggtg gtatcgcggc accgctgatt
     4321 gcaacgacgg gtgttagccc agagctgatg gttattgcgg tgggttccgg tagtgtgatt
     4381 ttctctcatg tgaacgatcc gggcttctgg ctgttcaaag agtactttaa cctgactatc
     4441 ggcgagacca tcaaatcctg gtcgatgctg gaaaccatta tctcggtgtg cggtctggta
     4501 ggctgtctgc tgctgaatat ggtgatttga taacactgac tgccggatgc atcgcatccg
     4561 acatcaaccg cactctactt cttcttcgct gcagctctgc gccgtctgtc caaatccttc
     4621 agcaacttgt tcacgccatc atcggcaaac atcgactcaa gcgttgcgga aagcttgcgt
     4681 cgccagtttt tatactggta actggtgcca ggaatattca ccggttcggc catatccagc
     4741 cagtcttccg gctgtagtcc taacagagca ctgttactgt cggcaatgta gcgctgcaaa
     4801 ccacggttca gcgtcggcgt catcgacatc aacgatgcct tatgcccggc acgttttggc
     4861 agacaaccat atttatgcag tgcatccagc agcccttgct tcgccagttc gcgatcctga
     4921 tacagaccgc gcagtaccac ttcatccgga tacagcccca gggttttgcc cagcgttaga
     4981 tccccgctct cccagtaacc acgcagcgtt ggcaggtcat gtgtcgccgc aaccgccatc
     5041 gactgctccg gatacgcttt cggtgcacgg aacgtcttct catggtcgtt ttcgaaatag
     5101 agcactttgt aagagtacac accgctgctg cgcagcttac caacaatctc taccggtacg
     5161 gtaccgagat cttcaccaat caccatacag cgatgacgtt tactttcgag tgccagaatc
     5221 gagagcagat catccaccgg atagtgaaca tacgcgccct gatctgccgt ctcgccatac
     5281 ggtatccacc acaaacgcag catcgacatc acatggtcaa ttcgtaatgc gccgcagttt
     5341 tgcatattgg cacgcaacag ctcgataaac ggttcatagg cacgcgcggt gatgatatgc
     5401 gggtccattg gcggtaatcc ccagttctgc cccaacgggc cgaggatatc cggcggcgcg
     5461 ccaaccgatg ctttcaggca atatagttca cggtcacacc aagtttccgc cccaccttcc
     5521 gctacgccaa ccgccagatc acgatacaag ccaatcggca tttcatagcc ctggcttatc
     5581 tcccagcagg cggcaaactg gctgtaagcc agccactgca accagagata aaaatcgacg
     5641 tcatcacgat gttcttcgca gaactgacgc acttctggtg aatccacgtt cttatacatc
     5701 tctggccatg caggccagcc ccagcgcatt tcgtcctctt tcacttgctg ggcatgtagc
     5761 gcatcaaagg cagcctgcca gaacaggctg tcgccctgct ctgcaacaaa ctggcgaaac
     5821 gcggccatct gctcatcatc acgttgcgcg aaacctttcc acgccattcg taatgctgtc
     5881 atttttaggg cggtaaccgt ggagtaatcg acccagtcgg catcgcgcgc ctgttgcagc
     5941 gtctgttgcg tggtcggcaa ctgccaccag gcctgagcct cttcgctaag atggaaatct
     6001 tcaacggcgt taacgtcgat ataaatcaca ttcagccaac ggcgagaaga cgggctgtat
     6061 gggctggcgc tctccggatt tgccggatag agcgcatgaa tcgggttcag gccaatgaac
     6121 gacccgccac gttttgccac atccaccagc atcgctttga gatcgccaaa atccccaata
     6181 ccccagtttt tttccgatcg cagcgtataa agctgaacgc aggcacccca cagcttttgt
     6241 ttattcagca aagcctgcgg ttcgtaacaa cgtttcgggg cgacaatcac ccggcaatgc
     6301 gcgcgctggt cgtcctgggt gagtgttagc gtgtgataac cttccggcag cttcgtcggt
     6361 agattgaacg ctttgccccc cgttacatgg cctttgtact gcgttccttc ttcggtggtc
     6421 agctgccagc tatattcgcc gctgccctcc accaccatcg gcattttttt gccgctggta
     6481 taaaccatga cattcgggac tggcgttacc gccactttcg tggtggtacg ttgatgcatc
     6541 gcgtcaagca aacgccgttt ggtttcggcg ctaatcgact gcggtttacc gtgggcattg
     6601 atgtaattgg ggctaatccc cgccgccagc gcggcattat ccagacgttt gctttccatc
     6661 gagcttcctt agcgttttgc ctgccagata cgagcctgat aatcgcgaat agagcgatcc
     6721 gagctaaaca taccgcagcg ggcggtattg aggatcgccg cgcgagtcca ggcctcctgg
     6781 tcgcggtaca gcacatccac ctgcttttgt gcctcaacat aggctgcgaa atccgccatc
     6841 accagatacg gatcgccgcc ctgtttgccg atactgtgca gcatctggtc gaaggcatgc
     6901 ttatcgccgt cgctgtattt accgctttcc agctctttca atactgcgtc cagcacctta
     6961 tctttcttcc gccatttcac cgggtcgtag cctttggcca gaatagcctt cacttgttcc
     7021 acggtatgac caaaaataaa gatattttct tcaccgactt tctcggcgat ttcaacgttc
     7081 gcaccatcca gcgtaccgac agtaagcgca ccattgagcg ccagtttcat attgccggta
     7141 ccggaagctt ctttacctgc agtcgaaatt tgttcggaga tatccgccgc cgggatcagt
     7201 ttttccgccg ccgaaacgca ataatccggc aggaacacca ccttcaactt atcgccaacc
     7261 agtggatcgt tgttgatcac gtcagccact ttgttgatcg caaagataat attcttcgcc
     7321 aggtagtagc ccggtgccgc tttcgcgccg aagaggaaga cgcgcggtac gcgatcagcc
     7381 tgcaggtttt cacgaatttc tttgtacaac gccagaatat gcagcagatt caggtgctgg
     7441 cgtttgtact catgcagacg tttgatctga atatcgaaaa tcgcctgtgg attgatctca
     7501 ataccggtac gaactttcac aaactccgcc agacagactt tattcgcctg cttgatctcg
     7561 cgatattgct gacggaattt cgcatcatca gcgaattttt ccagattgat cagctgatcg
     7621 agatcgttag cccactcttt ttgcagtgat ttatccaaca gagccgccag tgcagggttg
     7681 cactgtttga tccagcgacg tggggtaata ccgttggtaa cgttatggaa tttgttcggc
     7741 catagctggt gatattccgg gaacagatct ttcaccacca gatccgagtg cagcgccgca
     7801 acaccgttca ccgcgaaacc gccaaccaca cacaggttcg ccatatgcac ttgtttatcg
     7861 tgcaccaccg ccagtttggc ccacactttt tcatcgcccg gccaggtttt ctccaccagc
     7921 gttttaaagc gagtattaat ttcgttaata atctgcatat ggcgcggcag taagcctttc
     7981 accagtttca catcccagcg ttccagcgct tctggcatca gggtatggtt ggtgtaagcg
     8041 aaagttttgc tggtgatggc ccaggcgtca tcccagctca tctggtgctc atcggtcagc
     8101 acgcgcagca gttctggaat cgcgatagtt ggatgggtat cgttcagctg aataacttcg
     8161 taatccgcca gttcgtgcag tttacgcccc gccagatgat ggcgacgcaa aatatccgct
     8221 accgaacagg cacactggaa gtattgctgc atcaggcgca gctttttacc ggcagtatgg
     8281 ttgtcgtttg gatagagaac tttggtcagt ttttccgcat tgatgccttg ctgttcggcg
     8341 cgcaggaaat caccgtcgtt aaatttagtt agatcaaacg gatgcgcatg cgtcgcctgc
     8401 cacagacgca gcggctgcgc cacgccatta cgatagccga caacggggag atcccacgct
     8461 tgaccggtaa tggtaaactc cggctcccag cgtccgtctt tcatcacttt accgccaatc
     8521 cctacctgca catccagtgc ttcgttgtgg cggaaccacg ggtagttact gcgatgccag
     8581 tcatccggca cttcaacctg tttgccatcg acaaaagact ggcggaacaa accatattga
     8641 tagttcagac cgtaacccgt cgcagactga ccgacagttg ccattgagtc gaggaagcac
     8701 gccgccagac gtcccagacc accgttaccc agcgccgggt cgatctcttc ttccagcagg
     8761 tcagtcaggt tgatgtcata agccttcaac gaatcctgta catcctgata ccagccgaga
     8821 ttcaacaggt tgttgcccgt cagacgacca atcaaaaact ccattgagat gtagttaaca
     8881 tgtcgctgat tcgccaccgg tttggcgaat ggctgagcac gcagcatttc ggccagtgct
     8941 tcactcactg ccagccacca ctggcgagga gtcatttcag ccgcagaatt taagccataa
     9001 cgctgccact gacgtgaaag cacttcctga aattgcttat cgttaaaaat aggttgtgac
     9061 ataggagttc cacttttctt agattttcaa cacaacgtta tcgctagttt gccaggctcg
     9121 atgttgacct tcctcatcct gcgggggatt aggcagggag gagttgcggg gatgagcaag
     9181 gaaatgtgat ctcgaccact taaagctagt gcaaaccaca ggattagcag caaatcaatg
     9241 ccatacagcg cagaaaatct gtatctaagt gcaaaaaatg gccgttgcgt attttcaaaa
     9301 agcggaaggt aactctataa attaagtaaa ggagtgaaac agtttcacaa gtaaaatatc
     9361 cagtgtgctc catctcattc ttaatagatt tattaagatc atctttttag atggcacttt
     9421 catcaggaat gaagaagaaa cccttgctta aatgaatctg atgaacataa gggaaaccag
     9481 tattcacgct ggatcagcgt cgttttaggt gagttgttaa taaagatttg gaattgtgac
     9541 acagtgcaaa ttcagacaca taaaaaaacg tcatcgcttg cattagaaag gtttctggcc
     9601 gaccttataa ccattaatta cgaagcgcaa aaaaaataat atttcctcat tttccacagt
     9661 gaagtgatta actatgctga ttccgtcaaa actaagtcgt ccggttcgac tcgaccatac
     9721 cgtggttcgt gagcgcctgc tggctaaact ttccggcgcg aacaacttcc ggctggcgct
     9781 gatcacgagt cctgcgggct acggaaagac caccctcatt tcccagtggg cggcaggcaa
     9841 aaacgatatc ggctggtact cgctggatga aggtgataac cagcaagagc gtttcgccag
     9901 ctatctcatt gccgccgtgc agcaggcaac caacggtcac tgtgcgatat gtgagacgat
     9961 ggagcaaaaa cggcaatatg ccagcctgac gtcactcttc gcccagcttt tcattgagct
    10021 ggcggaatgg catagcccac tttatctggt catcgatgac tatcatctga tcactaatcc
    10081 agtgatccac gagtcaatgc gcttctttat tcgccatcaa ccagaaaatc tcaccctagt
    10141 ggtgttgtca cgcaaccttc cgcaactggg cattgccaat ctgcgtgttc gtgaccaact
    10201 gctggaaatt ggcagtcagc aactggcatt tacccatcag gaagcgaagc agttttttga
    10261 ttgccgtctg tcatcgccga ttgaagccgc agaaagcagt cggatttgcg atgacgtttc
    10321 cggttgggcg acggcactat agctaatcgc cctctccgcc cggcagaata cccactcagc
    10381 ccataagtcg gcacgccgcc tggcgggaat caatgccagc catctttcgg attatctggt
    10441 cgatgaggtt ttggataacg tcgatctcgc aacgcgccat tttctgttga aaagcgccat
    10501 tttgcgctca atgaacgatg ccctcatcac ccgtgtgacc ggcgaagaaa acgggcaaat
    10561 gcgtctcgaa gagattgagc gtcaggggct gtttttacag cggatggatg ataccggcga
    10621 gtggttctgc tatcacccgc tgtttggcaa cttcctgcgc cagcgctgcc agtgggaact
    10681 ggcggcggag ctgccggaaa tccaccgtgc cgccgcagaa agctggatgg cccagggatt
    10741 tcccagcgaa gcgattcatc atgcgctggc ggcaggcgat gcgctgatgc tgcgcgatat
    10801 tctgcttaat cacgcctgga gtttgttcaa ccatagcgaa ctgtcgctgc tggaagagtc
    10861 gcttaaggcc ctgccgtggg acagtttgct ggaaaatccg cagttagtgt tattgcaggc
    10921 gtggctaatg caaagccaac atcgctatgg cgaagttaac accctgctag cccgtgctga
    10981 acatgaaatc aaggacatca gagaaggcac catgcatgca gaatttaacg ctctgcgcgc
    11041 ccaggtggcg attaacgatg gtaatcagga tgaagcggaa cggctggcaa aactggcact
    11101 ggaagagctg ccgccgggct ggttctatag ccgcattgtg gcaacctcgg tgctgggtga
    11161 agtgctgcac tgcaaaggcg aattgacccg ctcactggcg ctagtgcagc aaaccgaaca
    11221 gatggcacgc cagcacgacg tctggcacta cgccttatgg agtttaattc agcaaagtga
    11281 aattctgttt gcccaagggt tcctgcaaac cgcgtgggaa acgcaggaaa aagcgttcca
    11341 gctgatcaac gagcagcatc tggaacagct gccaatgcat gagtttctgg tgcgcatccg
    11401 agcacaactg ttatgggcct gggcacggct ggatgaagcc gaagcgtcgg cgcgtagcgg
    11461 gattgaagtc ttgtcgtctt atcagccaca gcaacagctt cagtgcctgg caatgttgat
    11521 tcaatgctcg ctggcccgtg gtgatttaga taacgcccgt agccagttga accgtctgga
    11581 aaacctgctg gggaatggca aatatcacag cgactggatc tctaacgcca acaaagtccg
    11641 ggtgatttac tggcaaatga ccggcgataa agccgccgct gccaactggt tgcgtcatac
    11701 ggctaaaccg gagtttgcga acaaccactt cctgcaaggt caatggcgca acattgcccg
    11761 cgcacaaatc ttgctgggcg agtttgaacc tgcggaaatt gttctcgaag aactcaatga
    11821 aaatgcccgc agtctgcggt tgatgagcga tctcaaccgt aacctgttgc tgcttaatca
    11881 actgtactgg caggccggac gtaaaagtga cgcccagcgc gtgttgctgg acgcattaaa
    11941 actggcgaat cgcaccggat ttatcagtca ttttgtcatc gaaggcgaag cgatggcgca
    12001 acaactgcgc cagctgattc aacttaatac gctgccggaa ctggaacagc atcgcgcgca
    12061 gcgtattctg cgagaaatca atcaacatca tcggcataaa ttcgcccatt tcgatgagaa
    12121 tttcgttgaa cgtctgctaa atcatccgga agtgccagaa ctaatccgca ccagcccgtt
    12181 gacccaacgt gaatggcagg tcctggggct gatctactcc ggttacagca atgaacaaat
    12241 tgcgggcgaa ctggaagtgg cggcaaccac catcaagacg catatccgca atctgtacca
    12301 aaaactcggc gtcgcccacc gtcaggctgc cgtgcagcac gcgcagaaac tgctgaagat
    12361 gatgggttat ggggtgtgag tttagccgga taatgcattg gatccggttt acatctttac
    12421 atcattcaat actcacccgc gttacgccat ctgtttctat caaaccaaac cgcaccggca
    12481 agaaacgctc caccaccgcg atattggtca gcagatggca tgaaggatgg gcgaccgtaa
    12541 attctcccgc gcccgccagc gccatcggca gtaccagttg gtcggcgaga tattccccca
    12601 ccgccgctgg gcttgccagg tagcgtttca cctctttcac taactgcgcg gctaccacct
    12661 cggcactgac gcgcttttca ccgacgacga aaaagcgttc agtgatattt tcactttcga
    12721 cttccagcga gacggtatta cccggcccct ggtcgcgcgg caggttatga atattctgtt
    12781 catgcaggga aaaaatcccc gccagtgtag cgatttcacg ctcagcaaca tggcgcggca
    12841 caccagctaa tagaacttct ccacgcatct gcacaatgtt cccgcgctcg ccaagttgca
    12901 aggtgctaaa cgatgccacg ggcgagactt ccgttgccac cacaccgcct ccggcaggat
    12961 aaaaaccgtg acgtaacagc gtggtttgct gatgaattcc tattttcgcc agcagcggct
    13021 ccagcacccg gcggataaaa tccgcaggcg gcgccgacgg gttatcggtg ccgccgctca
    13081 cttcaacacg cgaaggtcca tcggcaaacc acagcgcggg cagcaccgtt tgcagcacca
    13141 gcgtacaact tccggcgcta ccgatagcaa agcggtaatc gccgccgcgc acggtgccag
    13201 gccggaagac cagacgctgc gatcccagct ccgcgccttc caccgttgcc ctacaaattt
    13261 ccgcagccgc ttttaccgcg gtcagatgct ggcgcagcag ccccggtttc gcccgtccgg
    13321 ctcgaatgcc agtgatggta aatggctggc cggttatcat cgacaggctc agcgccgagc
    13381 gcaggatctg cccgccgcct tcgccctgtg cgccatccag cgcaatcatc cttttcatca
    13441 tttatccttt tacgcacacc acctgacgca gggtgtagat aacttccacc agatcgcttt
    13501 gtgccgccat caccgcatca atatctttat acgccatcgg gatttcgtcg atcacttcgg
    13561 catctttacg gcattccaca tgcgcggtgg cacgaatttg gtcttccacg ctgaacagtt
    13621 ttttcgcttt agttcggctc attacccgcc cggcaccgtg gctgcacgaa cagaacgact
    13681 cttcatttcc cagcccacgg acgataaagc tttttgctcc catcgaaccg ggaataattc
    13741 catattgacc agcacgcgca gacaccgcac ctttacgcgt cacgtagatc tcttcaccaa
    13801 agagctgttc tttttgcaca tagttgtggt gacagttgat ctcttccatc gccagcgttt
    13861 gtggctgtct gaccgttttc tgcgtaatgc tctgcaacgc cgttaccaca ttttccatca
    13921 tcgcatcgcg gttaaggctg gcgaaaagct gcgcccaggc cacggctctt aggtaatcat
    13981 caaagtattc cgtaccttcc ataaagtacg ccagatcacg cgacggcaac gtctcaagtt
    14041 ggtcctgcat ctctttttgc gccagatcga taaagtaagt cccgatggcg ttaccaattc
    14101 cgcgtgaacc ggagtgcagc ataatccaca cctgctccga ctcatcaagg cagatttcaa
    14161 taaagtggtt acccgttccc agcgttccca ggtgtttata gttgttagtg ttaaggaaac
    14221 gcggatattt ttgcgttaac cactgataac cggcctccag ctcagcccat ttcgcatcga
    14281 cgttaaaagg cgggttttcc catgcgcctt tatcacgttt acaacgcccg gtagtacgcc
    14341 cgtgtggtac ggccgtttca atcgcttgac gcagctccgc caggttttca ggcaggtctg
    14401 ccgccgttaa cgcggtacgc agcgcgttca ttccacagcc aatatccacg cccaccgccg
    14461 ccggaataat cgcccctttg gtcgggatca cgctaccaat ggtggaacct ttacccaggt
    14521 gtacatcagg cattaccgca atatgtttga aaataaacgg catcttcgcc gtattaataa
    14581 gttgctgacg cgcatcggct tctaccggca cgcctttggt ccacattttt accggggcat
    14641 tttcagtggt cagtaattcg taattcattt tgtttttctc ttttcgttgt ttgcggtcct
    14701 gataattgca accgtcgtgc cagaaaatta aataagcaga ttaatttatt gtttctaaaa
    14761 agattatatc tttatatccg taaacggaga tttcccgcaa agccgattta ccgataatga
    14821 aatatcgcct tttataagga tatctaagat gcgtaaaaca gtggcttttg gctttgtcgg
    14881 taccgtgctg gattatgccg gacgcggcag tcagcgctgg tcaaaatggc gtccgtcact
    14941 ctgtatatac cagcaagaat cgttggtcat cgatcgactg gaattattgc acgatgcccg
    15001 ctcgcgctcg ctatttgaaa cgcttaaacg cgatatcgcc agcgtttcgc cagaaacaga
    15061 agtggtgggc gttgagattg aactgcataa cccgtgggat ttcgaagagg tttacgcctg
    15121 cctgcatgat ttcgcccgtg gttacgagtt tcagccagaa aaagaagact atttaattca
    15181 catcaccacc ggcacccacg tcgcgcagat ttgctggttt ctgctggcgg aagcgcgtta
    15241 tctacccgcc cggttgatac aatcttcaac tccgcgcaaa aaagaacagc cgcgcggccc
    15301 aggtgaagtg acgattatcg atctcgattt aagccgttat aacgccatcg ccagccgctt
    15361 tgccgaggaa cggcagcaaa cgctcgattt cctcaaatcc ggtattgcca cgcgtaaccc
    15421 ccacttcaac cgcatgattg agcagatcga aaaagtggcg attaaatccc gcgcgccgat
    15481 tctgcttaac ggcccgaccg gcgcaggtaa gtcatttctg gcgcgacgca tcttcgagtt
    15541 aaaacaggca cggcatcagt ttagcggcgc gtttgtggaa gtgaactgcg ccaccctgcg
    15601 cggcgatacc gcgatgtcga cgctgtttgg tcatgtgaaa ggcgcgttta ccggggcgcg
    15661 ggaatctcgt gaaggattat tacgcagcgc caacggcgga atgttgtttc ttgatgagat
    15721 tggcgaactg ggcgcagacg aacaggcaat gctgctgaaa gctattgaag agaaaacctt
    15781 ttacccgttt ggcagcgatc gccaggtgag cagtgatttt cagcttatcg ccggaacggt
    15841 gcgcgatttg cgccagctgg ttgccgaagg caaattccgc gaagatctgt acgcgcggat
    15901 caatctctgg accttcaccc tgccgggtct acgccagcgc caggaagata ttgaaccgaa
    15961 cctggattat gaagtggagc gccacgccac actcactggt gacagcgtgc gttttaacac
    16021 cgaagcgcgg cgtgcctggc tggcctttgc aacatctccc caggcaacat ggcgcggtaa
    16081 ctttcgcgaa ctttctgcca gcgtcacgcg gatggccacc tttgccacta gcggacgcat
    16141 cactctggac gtggtggaag atgagataaa ccgtctgcgc tataactggc aggagagccg
    16201 cccctccgcg cttacggcgt tgctgggcgc agaggcagaa aacatcgatc tcttcgaccg
    16261 tatgcaactg gaacacgtta tcgctatctg ccgccaggca aagtcgcttt ctgctgccgg
    16321 acgacagctt tttgatgttt cgcgccaggg caaagccagc gtcaatgacg cggatcggct
    16381 acgcaaatac ctggcgcgtt ttggtctgac gtgggaagcc gtgcaggatc agcacagctc
    16441 cagttgaata tggtggtccg tcagcacctg catcacgctt actggcggcg gggcgtcggt
    16501 gtagacggca tcaaccatgc tgatgctccc catattgacc atcgcattac ggccaaattt
    16561 tgaatggtca accaccagca taacgtggcg cgagttctca ataatggcgc gcttggtacg
    16621 aacttcgtgg taatcgaact ccagcagcga gccgtcgctg tcgatgccgc tgatccccag
    16681 aatgccgaaa tcaaggcgga actgggagat aaaatcgagc gtcgcttcgc caatgatccc
    16741 gccatcgcgg ctacgtaatt cgccaccggc gagaataatg cggaaatctt cttttaccat
    16801 caacgtgtta gcaacgttga gattgttggt gacaatgcgc aaattgctgt gattgagcag
    16861 cgcgtgcgcc accgcttccg gcgtggtgcc gatatcgata aacagcgtcg agccattggg
    16921 gatttgctcc gccactttgc gggcgatgcg ctctttttct tcggtctggg tggctttacg
    16981 atcgtgccac ggcgtgttaa ccgaactgga aggcagcgcc gcgccgccat gatggcgcag
    17041 gatcaggttt tgctctgcca gctcgttgag gtcgcggcga atagtctgcg ggctgacgga
    17101 gaagtgctcc accagttcct cggtactgac ataaccctgc tgtttaacca gttcgataat
    17161 accgttgtga cgttgtgttt gtttcattta taaatccctg gaattatttt cgttttcgcg
    17221 cattgagcga atcaacaaaa gccatcgcta aacccacggc taatccggcg atgtgtgctc
    17281 cgttcgccat cgacatccca aacaaatcaa accatccggc gacaatccag atcagcgcaa
    17341 agataattaa cccacgttgc aggtaaatgc cactttgcgg atcgcgttcg ccacgtagcc
    17401 agacgtagcc catcagcgca tacaccacgc cagaaagccc gccaaaccac ggcccgctga
    17461 atttttgctg catatagccg cttaacaggg cgctgataag cgtaatgaca attagcttac
    17521 cgctaccgag gcgtttttcc accgcgccgc cgagatacca ccaccaaagc aggttaaaga
    17581 ggatatgcat cagcgagaag tgcattaacg cgtgggtgaa gtaacgccag aactcaaatt
    17641 tcagtgttgg atcgaatggc caggccagcc ataacatcac ttcctgatcg ccgagaattt
    17701 gcatggcaat aaacaccacc acgcaggcga tcatcatcac ccaggtcacc ggacctgcgc
    17761 gttcacgcaa ggcagcaaag aaaggataac ggcgataatg caggccactg ccggtatggc
    17821 ctgccagcca gctggccgcc agataacgcg gatctgccgg gttttcgaga aaacgcgcca
    17881 gctccgcccg cacgcgctcg gcctgggact catccgccag ccagacatcg ctttggttat
    17941 gttgttgaat cgtgaggata acaccctgcg tcgccatgta atcaacaaac gcctgcgcca
    18001 cgcgggggtt agcaaaagag gtaatcatca acatcgttgc tgtcgcttat tccacacaaa
    18061 aggggacagt ataaagcgtt acgcgccgta cgccacctct gcgggaaact gtcgctgcca
    18121 ggcttcaaag ccgccgtcaa tgctatagac cacatcgtag ccctgttgca gcagatactg
    18181 cgccgcccct ttgctgctat tgccgtgata acacatcacc atcaccggag tgtcaaagtc
    18241 gttatcacgc ataaacgcgc ccagcgtgtc gttggttaaa tggaaagcct gcaccgcatg
    18301 ccccatagcg aaactctgtg gatcgcgaat atcgaccagc accgcctctt tttcctgcaa
    18361 cttctggtgc gcgtcggcaa cgttaataca ttcgaactga tccatgcgtc tctctttctt
    18421 tacaaacaag tgggcaaatt taccgcacag tttacgtcga agcggcagat aaacgccata
    18481 atgttataca tatcactcta aaatgttttt tcaatgttac ccaaagcgcg attctttgct
    18541 aatatgttcg ataacgaaca tttatgagct ttaacgaaag tgaatgaggg cagcatggaa
    18601 accaaagatc tgattgtgat aggtggcggc atcaatggtg ctggtatcgc ggcagacgcc
    18661 gctggacgcg gtttatccgt gctgatgctg gaggcgcagg atctcgcttg cgcgacctct
    18721 tccgccagtt caaaactcat tcacggtggc ctgcgctacc ttgagcacta tgaattccgc
    18781 ctggtcagcg aggcgctggc tgaacgtgaa gtgctgctga aaatggctcc gcatatcgcc
    18841 ttcccgatgc gttttcgcct gccacatcgt ccgcatctgc gcccggcgtg gatgattcgc
    18901 attggtctgt ttatgtacga tcatctgggt aaacgcacca gcctgccggg atcaactggt
    18961 ttgcgttttg gcgcaaattc agtgttaaaa ccggaaatta agcgcggatt cgaatattct
    19021 gactgttggg tagacgacgc ccgtctggta ctcgccaacg cccagatggt ggtgcgtaaa
    19081 ggcggcgaag tgcttactcg gactcgcgcc acctctgctc gccgcgaaaa cggcctgtgg
    19141 attgtggaag cggaagatat cgataccggc aaaaaatata gctggcaagc gcgcggcctg
    19201 gttaacgcca ccggcccgtg ggtgaaacag ttcttcgacg aggggatgca tctgccttcg
    19261 ccttatggca ttcgcctgat caaaggcagc catattgtgg tgccgcgcgt gcatacccaa
    19321 aagcaggcct acattctgca aaacgaagat aaacgcattg tgttcgtgat cccgtggatg
    19381 gacgagtttt ccatcatcgg caccaccgat gtggaataca aaggtgatcc gaaagcagtg
    19441 aagattgaag agagtgaaat caattacctg ctgaaagtgt ataacgcgca ctttaaaaag
    19501 cagctaagcc gtgacgatat cgtctggacc tactccggtg tgcgtccgct gtgtgatgat
    19561 gagtccgact cgccgcaagc cattacccgt gattacaccc ttgatatcca tgatgaaaac
    19621 ggcaaagcac cgctgctgtc ggtattcggc ggtaagctga ccacctaccg aaaactggca
    19681 gaacatgcgc tggaaaaact gacgccgtat tatcagggta ttggcccggc gtggacgaaa
    19741 gagagcgtgc tgccgggtgg tgccattgaa ggcgaccgcg acgattacgc cgctcgcctg
    19801 cgccgccgtt acccgttcct gactgaatcg ctggcacgtc actacacgcg cacttacggc
    19861 agcaacagcg agctgctgct cggcaatgcg ggaacggtaa gcgatctcgg ggaagatttc
    19921 ggtcatgagt tctacgaagc ggaactgaaa tacctggtgg atcacgaatg ggtccgccgc
    19981 accgacgatg ccctgtggcg tcgcacaaaa caaggcatgt ggctgaatgc ggatcaacaa
    20041 tctcgtgtga gtcagtggct ggtggaatat acgcagcaga agttgtcgct ggcgtcgtaa
    20101 attaacgtaa ggtgatcagg tcagatttca atctggcctg agactgatga caaacacaaa
    20161 actgcctgat gcgctacgct tatcaggcct acgtggttta tgcaatatat tgaatttgca
    20221 tggtcttgta ggccagataa gacgttcacg tcgcatccgg catgaacaaa gcgcactttg
    20281 tcaaaaatct aacctacttt ttaattcagg aaattaccgc aaagcccacg ccatcatacc
    20341 caacgtaaca aaactcaggc acgttcccca ctcgccccga gaaaaatagc attaatgcgc
    20401 ccagcgtcag cataaaaata ttgagcagaa ggtaaattgt tggcgtaaag agtaaattgc
    20461 aaacctcagg aatagcggca agcccagccc ccagcatggc aataaccgct gcaaaaaatt
    20521 gcgtcagtat tgggatgccg aaaaaagtca ttaccagcga ggtcaaaatc cattttctat
    20581 tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga gtccccttgc
    20641 cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg g
//
LOCUS       DAJTRD010000071        20545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTRD010000071 DAJTRD010000000
VERSION     DAJTRD010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.071"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            246..587
                     /gene="raiA"
                     /locus_tag="OO315_002792"
     CDS             246..587
                     /gene="raiA"
                     /locus_tag="OO315_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5891638.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    690..815
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            691..738
                     /gene="pheL"
                     /locus_tag="OO315_002793"
     CDS             691..738
                     /gene="pheL"
                     /locus_tag="OO315_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5891639.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            837..1997
                     /gene="pheA"
                     /locus_tag="OO315_002794"
     CDS             837..1997
                     /gene="pheA"
                     /locus_tag="OO315_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5891640.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2040..3161)
                     /gene="tyrA"
                     /locus_tag="OO315_002795"
     CDS             complement(2040..3161)
                     /gene="tyrA"
                     /locus_tag="OO315_002795"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5891641.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3172..4242)
                     /gene="aroF"
                     /locus_tag="OO315_002796"
     CDS             complement(3172..4242)
                     /gene="aroF"
                     /locus_tag="OO315_002796"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5891642.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4452..4817
                     /locus_tag="OO315_002797"
     CDS             4452..4817
                     /locus_tag="OO315_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5891643.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4967..5485
                     /locus_tag="OO315_002798"
     CDS             4967..5485
                     /locus_tag="OO315_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5891644.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5475..6700
                     /gene="dgcN"
                     /locus_tag="OO315_002799"
                     /pseudo
     CDS             5475..6700
                     /gene="dgcN"
                     /locus_tag="OO315_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6716..7198
                     /locus_tag="OO315_002800"
     CDS             6716..7198
                     /locus_tag="OO315_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5891645.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7275..7622)
                     /gene="rplS"
                     /locus_tag="OO315_002801"
     CDS             complement(7275..7622)
                     /gene="rplS"
                     /locus_tag="OO315_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5891646.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7664..8431)
                     /gene="trmD"
                     /locus_tag="OO315_002802"
     CDS             complement(7664..8431)
                     /gene="trmD"
                     /locus_tag="OO315_002802"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5891647.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8462..9010)
                     /gene="rimM"
                     /locus_tag="OO315_002803"
     CDS             complement(8462..9010)
                     /gene="rimM"
                     /locus_tag="OO315_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5891648.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(9029..9277)
                     /gene="rpsP"
                     /locus_tag="OO315_002804"
     CDS             complement(9029..9277)
                     /gene="rpsP"
                     /locus_tag="OO315_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5891649.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9414..10775)
                     /gene="ffh"
                     /locus_tag="OO315_002805"
     CDS             complement(9414..10775)
                     /gene="ffh"
                     /locus_tag="OO315_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5891650.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10942..11733
                     /locus_tag="OO315_002806"
     CDS             10942..11733
                     /locus_tag="OO315_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5891651.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11754..13040
                     /locus_tag="OO315_002807"
     CDS             11754..13040
                     /locus_tag="OO315_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5891652.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(13095..13688)
                     /gene="grpE"
                     /locus_tag="OO315_002808"
     CDS             complement(13095..13688)
                     /gene="grpE"
                     /locus_tag="OO315_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5891653.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13811..14689
                     /gene="nadK"
                     /locus_tag="OO315_002809"
     CDS             13811..14689
                     /gene="nadK"
                     /locus_tag="OO315_002809"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5891654.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14775..16436
                     /gene="recN"
                     /locus_tag="OO315_002810"
     CDS             14775..16436
                     /gene="recN"
                     /locus_tag="OO315_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5891655.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16585..16926
                     /gene="bamE"
                     /locus_tag="OO315_002811"
     CDS             16585..16926
                     /gene="bamE"
                     /locus_tag="OO315_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5891656.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16988..17278)
                     /locus_tag="OO315_002812"
     CDS             complement(16988..17278)
                     /locus_tag="OO315_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5891657.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17268..17705)
                     /gene="ratA"
                     /locus_tag="OO315_002813"
     CDS             complement(17268..17705)
                     /gene="ratA"
                     /locus_tag="OO315_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5891658.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17876..18358
                     /gene="smpB"
                     /locus_tag="OO315_002814"
     CDS             17876..18358
                     /gene="smpB"
                     /locus_tag="OO315_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5891659.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18573..18935
                     /gene="ssrA"
                     /locus_tag="OO315_002815"
     tmRNA           18573..18935
                     /gene="ssrA"
                     /locus_tag="OO315_002815"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            19140..20381
                     /locus_tag="OO315_002816"
     CDS             19140..20381
                     /locus_tag="OO315_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5891660.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5305 a         5365 c         4820 g         5055 t
ORIGIN      
        1 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
       61 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
      121 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gatttccatc
      181 acacattttg acatcaggaa cggtatgctg aattcaccaa gacgggaaga caagaggtaa
      241 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
      301 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
      361 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
      421 gcgttctggt tgccagtggt aaacatgaag atatgtacac cgcaattaac gaattgatca
      481 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
      541 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
      601 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
      661 actgtactaa agtcacttaa ggaaacaaac atgaaacaca taccgttttt cttcgcattc
      721 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
      781 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
      841 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
      901 tagcgttact ggcagaacgg cgcgaactgg ccgtcgaggt gggaaaagcc aaactgctct
      961 cgcatcgccc ggtacgtgat attgatcgtg aacgcgattt gctggaaaga ttaattacgc
     1021 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     1081 aagattccgt attaactcag caggctttgc tccaacaaca tctcaataaa attaatccgc
     1141 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     1201 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     1261 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     1321 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     1381 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcactact gatttatcca
     1441 ccatcaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     1501 gttatccgca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     1561 cacaggcaaa atcaccgcat gttgctgcgt tgggaagcga agctggcggc actttgtacg
     1621 gtttgcaggt actggagcgt attgaagcaa atcagcgaca aaacttcacc cgatttgtgg
     1681 tgttggcgcg taaagccatt aacgtgtctg atcaggttcc ggcgaaaacc acgttgttaa
     1741 tggcgaccgg gcaacaagcc ggtgcgttgg ttgaagcgtt gctggtactg cgcaaccaca
     1801 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     1861 tctatctgga tattcaggct aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     1921 taggggaaat cacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     1981 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     2041 tactggcgat tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     2101 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     2161 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     2221 agattacgct ctgacgacat aatgatgtcg gcataaagct gcggatcctg agcaaacagt
     2281 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     2341 agctgaacat tttcttctgc caggtgcagc ccgtaagcaa aagtagcaaa gtggcgcaga
     2401 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg caaccgagcg
     2461 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     2521 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     2581 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     2641 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     2701 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     2761 tcccagtcat gttgctccag aatccgcacc tgataacccg atagtgtcag catcttctcg
     2821 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     2881 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa
     2941 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     3001 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcaccttg
     3061 cccacttcag caaccagttc cagacgcttc gctaataaat tcagcagcgc tttatcgact
     3121 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     3181 gcgagccgtc agctgcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat
     3241 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     3301 ctcagaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     3361 attaccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     3421 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     3481 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     3541 caggatcaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     3601 gttaatgcca acaaaacagt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     3661 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     3721 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     3781 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     3841 cagctcaagc aacaatttac gcgcgatttg cagcccggct tctacatcaa aagagccatc
     3901 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     3961 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagagctt taaatcgacg
     4021 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     4081 acgaggatcg cgcccggcga taatatctga aatggtttta cgcgagtcag caatctgggc
     4141 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     4201 ttcgtcggta atatgtacgt tattcagcgc gtctttatgc atgatggcga tcctgtttat
     4261 gctcgtttgc gatagttgct cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     4321 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gctttcaacc
     4381 cttgcttctg tatagataaa tttacatccc ctttaaaaac aatccgctat gctttgaaaa
     4441 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
     4501 gtcagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgtcg
     4561 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
     4621 aggcggatcg cggtctatac cttaaaggat atgccgaagg gcaaaagaaa acttgccaga
     4681 ccgattttac ttatgcccgg ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
     4741 ttgaaaatgc cagccaactg catgaagtat ggcaaaaaag ggctgatgaa aatgccagca
     4801 caatacgatt aaattaatta aaaccccaca aataaattaa acataagatt ttactcattg
     4861 tgttttattt ctcacattga tgacggtcgc atgaaaaaat gatattttaa tgagcatcct
     4921 gaaaaaatat cgatgcattt cgagcgaaga tggtggggat ccctgaatgc gtttttctca
     4981 ccgactgttc ttacttctga ttctcctgct gacgggagca ccaatactcg ctcaagagcc
     5041 ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt attgtcagtt acacccgctg
     5101 gcctgcgcta tcagggccac caaagttatg tatcttttca tcttcacgtt ttagtaccgc
     5161 cctacaagag aatgccgcga catctttgcc ttatctgcct gtcatcattc acactcaaca
     5221 agaggcgatg atttcaggct gcaatggttt ttattttggc aacgagtcac cgacattcca
     5281 gatggaatta accgaacaat atccgtcaaa ggcgttatta ttaattgccg aacaaaatac
     5341 cgagtgcatt attggtagcg ccttttgtct gatcatccac aataatgacg tcagatttgc
     5401 cgtaaacctg gatgcctcat cgcgtagcgg tgtaaaggtc aacccggatg tcttaatgct
     5461 cgcacggaag aaaaatgatg gataacgata attctcttaa taagcgcccc acgtttaaaa
     5521 gagcattacg caacatcagt atgaccagca tatttatcac tatgatgctg atctggttgc
     5581 tgctttccgt gacctcggtg ctgaccctga aacagtacgc gcaaaaaaac ctggcactga
     5641 cagcagcaac aatgacttac agtctggaag cagctgtcgt ttttgccgat ggccctgcag
     5701 caactgaaac actggcagcg ctgggccagc aagggcattt tcaactgcag aagtacgtga
     5761 taagcagcaa aatattctgg catcctggca ttacccccgt aaggatccag gcgatacttt
     5821 cagcaatttc ataagccact ggctcttccc tgcccccatc attcagccga ttcgtcacaa
     5881 tggtgaaacc attggcgaag tacgcttaac cgctcgcgac agttcaatca gccattttat
     5941 ctggttttcg ctcgccgtac tgaccggttg tattctgctg gcatcaggca tcgcaattac
     6001 cctcacccgc catttgcaca atggcctggt ggaagcactg aaaaatatca ccgatgtcgt
     6061 acatgatgtg cgttccaacc gcaatttttc ccgacgagtt tcggaagaac gtatcgctga
     6121 gtttcaccgc ttcgctctcg acttcaacag tctgctggat gaaatggaag agtggcagct
     6181 tcgtttacag gccaaaaatg cgcagcttct gcgtaccgcg ctacatgacc cattaaccgg
     6241 gctggctaac cgcgcagcgt ttcgtagcgg catcaatacg ttgatgaaca attccgatgc
     6301 ccgaaaaacg tcggcgttac tatttcttga tggcgataat ttcaaatata tcaatgatac
     6361 ctggggtcat gcgacgggcg atagggtctt gattgaaatc gcaaaacggt tagctgaatt
     6421 tggcgggctg cgacataaag cataccgcct gggcggcgat gaattcgcta tggtgctcta
     6481 tgatgtacag tcggaatctg aagtgcagca gatatgctca gcactgacac aaatctttaa
     6541 tctcccgttt gatcttcata atggccatca gaccaccatg acattaagca ttggttacgc
     6601 gatgaccatt gagcacgcct ctgcggaaaa attacaagag cttgccgatc acaatatgta
     6661 tcaggccaaa caccagcgtg ccgaaaagct ggtgagataa caaggatata tcgatatgat
     6721 aaagcacctg gtagcacccc tgattttcac ctcactaata ctgactggct gccagtcccc
     6781 tcagggaaag tttactcctg agcaagtcgc cgctatgcaa tcttatggat ttactgaatc
     6841 cgccggcgac tggtcgctgg gcttatcaga tgccattctg ttcgcaaaaa atgactacaa
     6901 attgctcccg gaaagccagc aacagatcca aaccatggca gccaaattgg cctcgacagg
     6961 gctaacacat gcccgtatgg atggacacac cgataactat ggtgaagaca gttacaacga
     7021 aggcttatca ttgaaacggg cgaatgtcgt ggccgatgca tgggctattg gtggacaaat
     7081 tccacgcagc aatctcacca cacagggttt aggaaaaaaa tatcccatag ccagtaacaa
     7141 gaccgcccag ggccgcgccg agaaccgccg cgtcgcagtg gtgattgcaa ccccttaaaa
     7201 cacgagcaaa cagacaaaaa aagggccagc caattggcca gcccttctta acaggatgtc
     7261 gcttaagcga aatcttagtt aagacgctct ttgatacgag cagccttacc agtacgctca
     7321 cgcaggtagt acagtttagc tttacgaaca gcaccacgac gtttgacaga aatgctgtca
     7381 actaccggag agtgagtctg gaagacacgc tcaacgcctt cgccgttgga aattttacga
     7441 acagtgaatg cagagtgcag accgcggtta cgaatagcga taaccacgcc ctcgaatgcc
     7501 tgcagacgtt ttttggaacc ttcaacaacc catactttca cttccacggt atcacccgga
     7561 cggaaggaag gtacgtcctg cttcatctgc tcttgttcaa gttgcttaat aatgttgctc
     7621 ataatttaat ctcttatcct gggtaaactg atctctcggg ggcttacgcc atcccatcat
     7681 gtttatgttg ctgttgtgcg tgttccgttt tgaactccgc cagcaacctt gcttgctctt
     7741 cagtcagagc caggttttcc agaagttcag gtcttctaag ccaggtacgg cccagcgact
     7801 gtttcaaacg ccagcgacgt atctcggcat ggttgcccga cagtaacact ggcggaactt
     7861 ccatcccttc taacacctca ggccgcgtat agtgcgggca atccagcaat ccttcagcaa
     7921 aggaatcttc cgttgccgag gcttcatgtc ccagtactcc cggaataaac cgggaaacgg
     7981 agtcaatcag cgtcattgct ggtaactcac caccactgag aacgtaatcg ccgattgacc
     8041 attcttcgtc aatttcggtt tggatcacgc gctcatctat accttcgtag cgaccgcaca
     8101 ccagaatcaa tttttgattc gttgccagtt cgctgacgcc cgcttgatca agcttgcgtc
     8161 cctgtggtga cagataaatc acctttgcgc cttcacccgc cgcggctttt gctgcatgaa
     8221 tggcgtcccg caagggttgc accatcatta acatccccgg tccgccgccg taaggacgat
     8281 cgtccacggt acggtgccgg tcatgcgtga agtcgcgagg actccaactc tggatgctca
     8341 gcaggccatt tttaactgcc cggccagtta ccccgtaatc ggtaattgcg cggaacattt
     8401 caggaaacag gctaattatg ccaatccaca tagcgccgcc ttttaccgtt tatccggtgg
     8461 tttaaaaacc aggatcccaa tctacttcga ttgaacgagt agtgagatcg actttcttga
     8521 taacctgccc atcgaggaac ggtacgagac gttccttgat accaaacgca tctttcaggt
     8581 ttgccttaat gacgagaacg tcattagatc cggtttccat catatcaacg actttaccga
     8641 gatcgtagcc ttcagtggtt actacctggc agcccatcag gtctttccag tagtagtccc
     8701 cctcttcaag ctgaggcagc tgcgatgaat ccacgacaat ttcacaattc gtcagcaggt
     8761 tcgccgcatc acgatcgtca acgcctttca gcttgatgat catgtcctga ttgtggtgct
     8821 tccagctttc cagctggact tgctgccact gacccgcctt ctggataaac cagggctgat
     8881 agtcaaaaat gctttcggcg tcttcggtgg aagaaaacac tctgagccac ccacgaatac
     8941 cgtaagacga acccattttt cccaaaacga tgggatcaac aggtgcttgc gcggtgagtt
     9001 gtttgctcat catgaccacc gtgacagatt aagctgcttt gtttacttct ttgatcagcg
     9061 cagcaacgcg atcagaaata gttgcgccct ggccaaccca gtgagcgatg cgatccagat
     9121 ccaggcgagt gccttcttct ttttcgctag cgattgggtt gaagaaacca acgcgctcga
     9181 tgaagcgacc gttgcgtgca ttacggctgt cagcgacaac aacctggtag aacggacgct
     9241 ttttagcgcc gtgacgtgct aaacgaatag ttaccataac atcctcttgt gtgaataaaa
     9301 caaccggacc ccatcgagga acggagtccg gtgtcatatt aaaagcccga aaattttact
     9361 gatttctgga gaaaatgcaa tcagcagtta ataactctgt cgcataaggt catttagcga
     9421 ccaggaaatc ccggcggcat cattcccttc atgcttctca tcatcttcgc cattccgcct
     9481 ttcttcattt tcttcatcat gcgctgcatg tcgtcgaact gcttcagaag gcggttaacg
     9541 tcctgtacct gcatcccgca accggcggcg atacgacgct tacgcgaacc tttgatgatt
     9601 tctggcttag cgcgctcttt catcgtcatt gagttgatga tggcttccat acgcaccagc
     9661 actttatcgt ccatctgcga cttgacgtta tccgggatct gccccatgcc cggcagtttg
     9721 cccatcaagc tggccatgcc gcccatattt ttcatctggc gcagttgctc aagaaagtcg
     9781 ttgagatcga agccgtcgcc tttttttagc ttgctggcta atttctctgc ctgcgcgcgg
     9841 tcaactttgc tttcgatatc ttcgatcagc gacagtacgt cgcccatgcc gagaatacgc
     9901 gacgcgatgc ggtccggatg gaacggctcc agcgcctcag ttttctcgcc aacaccgagg
     9961 aacttgatcg gtttgccagt gatgtgacga atagagagcg ccgcaccgcc gcgggcatcg
    10021 ccgtccactt tggtcaacac tacgccggta agcggtaacg cttcattgaa tgcttttgcc
    10081 gtattggccg catcctgacc ggtcatggcg tcaaccacaa acagggtttc aaccgggtta
    10141 atcgacgcat ggacttgttt gatctcgtcc atcatcgctt cgtcaacgtg cagacgacca
    10201 gcggtatcca ccagcagcac gtcgtagaat ttcagtttgg cttctttcag cgccgcgtta
    10261 acaatatcaa ccggcttctg accaacatca gaagggaaga aatcaacgcc cacctgctct
    10321 gccagcgtct caagctgttt gattgccgcc gggcgataga cgtcagcaga aaccaccagc
    10381 actttcttct tgtgcttctc gcgcaggaaa ttaccgagtt taccaacgct ggttgtttta
    10441 ccggcacctt gcaggcctgc catcagcacg accgcaggcg gttgcgcagc cagattcagg
    10501 gtctggttct cttcgcccat cgccgcaacc agttcgttac ggacgatttt gacgaactcc
    10561 tgccccggcg tcaggctctt attaacttca tgaccaaccg ctttctcttt tacgcgattg
    10621 ataaactcac gcactaccgg cagagcgacg tccgcctcca gcagcgccat gcgcacttcg
    10681 cgcagcgtat cttttacgtt gtcttcagtg aggcgtccac ggccactgat attgcgcagc
    10741 gtgcgcgaca aacgatcggt taaattatca aacattgtct ctcgcctggg gtggaaacgg
    10801 ttggccgcaa tcgcgacaca tcatcagtat tttgccgcag tataacatga aggcgtcttt
    10861 gttgttatgc aacggttgga gcagcgttca cctgacgcta tactgcttct ctttcttatt
    10921 gctcaaactg tcgacattac tatgcccgtt tttgctctgc tcgcgcttgt cgcctactcc
    10981 gtcagtcttg cgctgattgt tcccggtctg ctgcaaaaaa acggcggctg gcggcgcatg
    11041 gctattattt ctgcggtcat tgcgctggtc tgccacgcaa tcgctctgga agcccgcatc
    11101 ctgcccgacg gtgatagcgg acaaaacctc agcctgctga acgttggttc attggtcagt
    11161 ttggtgatct gtacggtaat gaccattgtg gcttctcgca atcgtggctg gttgctgcta
    11221 cccattgtct atgcctttgc gcttatcaac ctggcgctgg cgaccttcat gcccaatgaa
    11281 tacatcaccc atctggaagc aacgcctggg atgctgatgc acattggctt atcgctcttt
    11341 tcctatgcca cgctaattat cgccgccctg tacgcgctgc aactggcgtg gattgattac
    11401 caactgaaga acaagaagct ggcatttaac caagaaatgc cgccattgat gagtatcgag
    11461 cgtaaaatgt tccacatcac gcagattggc gtggtgctgc taacgctcac gctttgcact
    11521 ggcctgttct acatgcacaa cctatttagc atggaaaata tcgacaaggc tgtgctctct
    11581 atcgtggcgt ggtttgtcta tattgtgctg ctgtggggac attaccatga aggatggcgt
    11641 ggacgccgcg tcgtctggtt taacgttgcg ggcgcggtaa ttctgacact ggcctacttc
    11701 ggcagccgaa ttgtccagca gttaatcagc taaacccaga aaaggagttt cccctggaac
    11761 acatttctac tactacgttg atcattattc tgatcatcat ggtggtcatt tcagcctatt
    11821 tttccgggtc cgaaaccgga atgatgaccc tcaaccgcta tcgtcttcga catatggcga
    11881 aacagggtaa tcgctcggcc aaacgcgtcg aaaaattgct gcgtaagcca gaccgcctga
    11941 taagcctggt gttaatcggc aataacctgg tcaatattct tgcctccgcg ctcggcacta
    12001 ttgtcgggat gcgtttgtac ggcgatgcgg gcgtggcaat tgcgactggt gtgctgactt
    12061 ttgtcgtact ggtttttgct gaggtattgc cgaaaaccat tgccgcgctg tacccggaaa
    12121 aagtcgctta tccgagtagt tttctgctgg ctccgctgca aattttgatg atgccgctgg
    12181 tctggttgct gaatgctatc acccgtatgc tgatgcgcat gatgggtatc aaaaccgata
    12241 tcgtggttag cggctctttg agcaaagaag agttgcgcac tatcgtgcat gaatcgcgct
    12301 cacaaatttc ccgtcgcaat caggatatgc tgctgtcggt gctcgatctg gaaaaaatga
    12361 ccgttgatga catcatggta ccgcgcagtg aaattatcgg tattgatatc aacgatgact
    12421 ggaaatctat tctgcgccaa ctctcccact cacctcacgg gcgcatcgtg ctttaccgtg
    12481 attcgctgga cgacgccatc agtatgctgc gagtacgtga agcctggcgg ttgatgtcgg
    12541 agaaaaaaga gttcaccaaa gaaaccatgc tgcgcgccgc ggacgagatc tattttgtgc
    12601 ctgaaggtac gccgctcagc acgcagttgg taaagtttca gcgcaacaaa aagaaagtcg
    12661 gcctggtcgt caacgagtat ggagacattc aggggctggt gacggttgaa gatattctgg
    12721 aagagattgt tggcgatttc actacgtcga tgtcgccaac acttgccgaa gaggtcacgc
    12781 cgcaaaacga cggttcggtg attatcgatg gcaccgccaa cgtgcgggaa atcaacaaag
    12841 cctttaactg gcatctaccg gaagatgatg cccgcacggt taatggcgtc attcttgagg
    12901 cactggaaga gatccctgtc gcaggcaccc gcgtgcgtat tggcaagtac gatatcgata
    12961 ttctcgacgt acaggacaat atgattaagc aggtaaaagt ttttcctgtg aaaccgctgc
    13021 gcgagagtgt ggcggagtaa cgaaaacggc cctgcattcg catgcagggc cgtgaattat
    13081 tacgaaaaca gaaattacgc ttttgctttc gctacagtta ccatcgccgc acgaatcgta
    13141 cgaccattca gcgtataacc cttctgcata atgcccagta cgttacctgg cgcaacgtca
    13201 tcagattcca ccattgcgat agcctgatgc acattcgggt ccagcggcac gttagtttcg
    13261 gcgatcactt caacaccaaa cttacgcaca acatccagca tcgacttcag cgtcagctca
    13321 atgccttcaa ccatcgcaga catatccggg ttagctttat cagccacttc cagcgcacga
    13381 tccaggctat caatcaccgg cagcaattcg ttgatgaatt tctccagcgc gaatttgtgg
    13441 gctttttcaa tatccagttc agtacgacga cgcaggtttt ccatttcggc ttttacgcgc
    13501 aaaataccgt cacgttcacg ggtctgggct tcagccagct gagcttcgag attcgcaatt
    13561 ttttcatcgc gcggatccac ctgctcagca gaagcttctg gctcaactgc ctcaatctct
    13621 tcgtgctgat ccatgataat ttcttccggg gcttgcccct caggcgtttt ctgttcttta
    13681 ctactcatga atttctccgc gtttttttcg cattcatctc gctaacttcg cttattatgg
    13741 ggatcagttt cagggtttca agggaagcac tcacattgtc atcaatcttc gcaacaagga
    13801 cctcggaaaa atgaataatc atttcaagtg tattggcatt gtgggacacc cacggcaccc
    13861 tactgcactg acaacacatg aaatgctcta ccgctggctg tgcacaaaag gttacgaggt
    13921 catcgttgag caacaaatcg ctcacgaact gcaactgaag aatgtgaaaa ctggcacgct
    13981 cgcggagatt gggcaactgg ctgatctcgc ggtagtcgtt ggtggcgacg gtaatatgct
    14041 gggcgcggcg cgcacgctcg cccgttacga tattaaagtt attggaatca accgtggcaa
    14101 cctgggtttc ctgactgacc ttgaccccga taacgcccag caacaattag ccgatgtgct
    14161 ggaaggtcac tacatcagcg agaaacgttt tttgctggaa gcgcaagtct gtcagcaaga
    14221 ttgccaaaaa cgcatcagca ccgcgattaa cgaagtggta cttcatcctg gcaaagtggc
    14281 gcatatgata gagttcgaag tgtatatcga cgagatcttt gcgttttctc agcgatctga
    14341 tgggctaatt atttcgacgc caacaggctc caccgcctat tccctctctg cgggcggtcc
    14401 tattctgacc ccctctctgg atgcgattac cctggtgccc atgttcccgc atacgttgtc
    14461 agcacgacca ctggtcataa acagcagcag cacgatccgt ctgcgttttt cgcatcgccg
    14521 taacgacctg gaaatcagtt gcgacagcca gatagcactg ccgattcagg aaggtgaaga
    14581 tgtcctgatt cgtcgctgtg attaccatct gaatctgatt catccgaaag attacagtta
    14641 tttcaataca ttaagcacca agctcggctg gtcaaaaaaa ttattctaat tttacgccag
    14701 cctctttact gtatataaaa ccagtttata ctgtacacaa taacagtaat ggtttttcat
    14761 acaggaaaac gactatgttg gcacaactga ccatcagcaa ctttgctatc gttcgtgagc
    14821 ttgagattga ttttcatagc ggcatgaccg taataactgg cgagaccggt gcgggtaaat
    14881 ctattgcaat agatgccctc ggtctttgtc tcggtggtcg cgctgaagcc gacatggtgc
    14941 gtaccggcgc tgctcgcgct gacctgtgcg cccgtttttc tctgaaagat acgccagcgg
    15001 ccctgcgctg gctggaagaa aaccagcttg aagacgggca tgaatgtttg cttcgtcgcg
    15061 tgatcagcag tgatggtcgc tcccgtggtt tcatcaacgg tacagctgtt cctctgtcac
    15121 aactgcgcga actgggtcag ttgctgattc agatccatgg tcagcacgct catcaattac
    15181 tcaccaaacc tgagcaccaa aaattcctgc ttgatggcta tgccaatgaa acctctctac
    15241 ttcaggaaat gaccgcacgt tatcagttgt ggcatcaaag ctgccgtgac ctcgcgcatc
    15301 atcaacagtt aagtcaggaa cgcgccgccc gtgcggaact gctgcaatac caattaaaag
    15361 aacttaacga atttaatccg cagcccggag agtttgagca aatcgacgaa gagtacaaac
    15421 gtctggcgaa cagcggtcaa ttgctgacca ccagccagaa tgcattggca ttaatggccg
    15481 acggtgaaga cgcaaacctg caaagtcagc tttacacggc taaacaactg gtgagcgaat
    15541 tgattggcat ggacagcaaa ctgtccggcg tacttgatat gctggaagaa gctaccatcc
    15601 agattgctga agccagcgac gaactgcgcc actactgcga tcgtctggat ctcgatccca
    15661 accgactgtt tgaacttgaa cagcgcatct caaaacagat ttcgctggca cgtaaacatc
    15721 acgtcagccc tgaggcatta ccacagtatt accagtcgct actggaagaa cagcagcaac
    15781 tggacgatca ggccgactca caagaaacgc tcgcgctggc ggtaacgaaa catcatcagc
    15841 aggcactgga aaccgcgcgc gcattacacc aacaacgcca gcaatatgca gaagaacttg
    15901 cacagctgat caccgacagt atgcatgcgc tctcaatgcc gcatgggcag tttacgattg
    15961 atgtcaaata tgacgagcat cacctgggcg ctgacggtgc cgatcgtatt gagtttcggg
    16021 taaccaccaa cccaggtcag ccaatgcagc ctattgccaa agtcgcatcc ggtggtgaat
    16081 tgtcccgcat cgcactggca atccaggtca tcacggcgcg taaaatggaa accccggcac
    16141 tgatttttga tgaagtggat gtagggatta gcggtccaac agcggcagtt gtcggcaaac
    16201 tgctgcgtca actcggcgaa tcaactcagg tgatgtgtgt tacccacctg ccacaagtcg
    16261 cgggatgtgg tcatcaacac tattttgtca gcaaagaaac cgatggtgcg atgacagaaa
    16321 cacatatgca atctctggat aaaaaagcgc ggttacaaga gctggcgcgc ctgcttggtg
    16381 gcagtgaagt cacacgtaac acactggcga atgcgaaaga actgcttgca gcgtaaactt
    16441 ttttcctgct tcacggtcag agtaaacagc aaaacgccgt aagaccggaa agcaaaaggt
    16501 tttaaagtga tgaaaggtct attatcatcg gcatattaca gatgagccac gtactgctcg
    16561 ggcccgaaaa ggaatcaaat cactatgcgc tgtaaaacgc tgactgctgc agcagcagta
    16621 ctattgatgt taaccgcagg ctgttccact ctggagcgag tggtttaccg tcctgacatc
    16681 aaccagggga actatctgac cgctaacgac gtatccaaaa tacgtgttgg catgacgcaa
    16741 caacaagttg cgtacgcatt gggtacaccg ctgatgtccg atccatttgg tacgaatacc
    16801 tggttctatg tcttccgcca gcaaccaggt catgaaggtg taacgcagca aacgctgacg
    16861 ctgaccttta acagtagcgg tgtgttgacc aatattgata acaaacctgc gctgagtggt
    16921 aactaataat aaagttgctc tcaaagacgt taaaaaaggt gctcaatgag cacctttttt
    16981 ctgtctgtta tttattcgct gatttttctg ctcgttgcct gcgaagctct ttcggatcgg
    17041 caatgagagg gcgataaatc tccacccgat cgccatcatg cacactatcg cttagttttg
    17101 ccggacggct gtaaatgccg actttgtttt tagttaaatc gatatcggta cgcaattcca
    17161 gcaagccact ggcgcgaata gcttcttcaa ccgtcgcgcc ctcctgcagc gtcactcgct
    17221 gcaggtattg cttctcaggt agcgcataag ccacctcaac ggcaatttta cctagcactg
    17281 taaacctctt tcgcacgaac cgtaaaagcc tggaccatat tagccgccag ctctttaaac
    17341 acgcgaccaa aggcgagttc aatcaactta ttggtaaact caaagtcgag atgaaactcg
    17401 atacgacacg cctcctggct cagcggcgta aacttccatc caccaatcaa tttcttgaac
    17461 ggcccatcca ccagattcat aagaatactt tggttactgg tcaactggtt gcgggtagta
    17521 aacgttttgc tgatcccagc cttagagaca tctaccgccg cagtcatctg cccaggagtg
    17581 gactccagaa tccgacttcc ggtacaacct ggcaaaaact gaggataaga ctgaacgtca
    17641 ttcactaact gatacatttg ctccgcgctg tagggtacca gtgcggtccg gctaatctga
    17701 ggcataacaa tttccatcaa caaaaatcca acaaataata tcatttatcc tgctgtaaaa
    17761 aaaacgctat cccggcgctg ggtaacatcg ggttcatgct aagatagagc cttgtccccc
    17821 gcaggattga tatggggtat tttcgatttc agattaccga tgattcacga cgcttatgac
    17881 gaagaaaaaa gcacataaac ctggttcagc gaccatcgcg cttaacaagc gcgcccgtca
    17941 cgaatacttt atcgaagaag agttcgaagc gggacttgcc ctgcaaggct gggaagttaa
    18001 atccctgcgc gcaggaaaag ccaatatcag cgacagctac gtccttctgc gtgacggaga
    18061 ggcatttctg tttggcgcta acatcacgcc aatggccgtg gcctccacgc atgtggtgtg
    18121 cgatcctacc cgtacccgca agttacttct caaccagcgc gaactggact cattgtacgg
    18181 tcgcgtcaat cgagaaggct ataccgtagt ggcgctctcc ctgtactgga aaaatgcctg
    18241 gtgcaaagtg aaaatcggcg tcgccaaagg taaaaaacag cacgataaac gttcagatat
    18301 caaagaacgc gaatggcagg tggataaagc acgcatcatg aaaaacgccc accgttaaac
    18361 ctgcactcca attattgacc agttcctcac cgcgcctccc tctccggcgg cgcgaatgaa
    18421 catcttattg gctatcacat ccgacacaaa tgttgccatc ccattgctta atcgaataaa
    18481 aatcaggcta catgggtgct aaatctttaa cgataacgcc attgaggctg gtcatggcgc
    18541 tcataaatct ggtatactta cctttacaca ttggggctga ttctggattc gacgggattt
    18601 gcgaaaccca aggtgcatgc cgaggggcgg ttggcctcgt aaaaagccgc aaaaaatagt
    18661 cgcaaacgac gaaaactacg ctttagcagc ttaataacct gcttagagcc ctctctccct
    18721 agcctccgct cttaggacgg ggatcaagag aggtcaaacc caaaagagat cgcgtggaag
    18781 ccctgcctgg ggttgaagcg ttaaaactta atcaggctag tttgttagtg gcgtgtccgt
    18841 ccgcagctgg caagcgaatg taaagactga ctaagcatgt agtaccgagg atgtaggaat
    18901 ttcggacgcg ggttcaactc ccgccagctc caccaaaatt ctccatcggt gattaccaga
    18961 gtcatccgat gaagtcctaa gagcccgcac ggcgcaagcc ctgcgggctt ttttgtgctc
    19021 cgaatttgtc ccgcgaagtc tgatgccaac taattaaatc cgatcctttt aggcaccttg
    19081 ttaggcactt cataaagcta tattgtcttt gaggtgccta aaactatgga aacccggcaa
    19141 tggcaagaca aaccaaacct ctatccgtta aggaaatcga atctgctaaa cccaaggaag
    19201 cggactacgt tctctatgat ggcgatggcc ttgagctact cattaaatcc agcgggagta
    19261 aaatctggca gtttcgctac attcgccctg tcaccaagac acgagcaaag aaaagcatag
    19321 gcccttaccc gtcagttacc cttgccgatg ccagaaacta ccgtgcagag tcccgctcac
    19381 tcctagctaa acagattgat ccgcaggaac atcagcaaga acaattgcgt agttcgctgg
    19441 aagccaaaac caatactttc cagctcgtag ctgaacgttg gtggaatgtg aagaaagcca
    19501 gtgtgacaga ggactatgcg gaagatatct ggcgctctct tgaaagagat gtctttcctg
    19561 cgattggcga cgttagcgtt acagatatta aagctcatac actggttcag gccgtacaac
    19621 cggttcaggc cagaggagca ctggaaacgg ttcgtcgcct gtgtcaacgt attaatgagg
    19681 tcatgatcta tgcccagaac acagggctga ttgatgctgt tcccagcgtt aatatcggta
    19741 aagccttcga aaagcctcag aaaaagaaca tgcccagcat tcgaccggat cagctatctc
    19801 aactgatgca gacaatgcga acagccagca ttagcctttc cacacgctgc ctgttcatgt
    19861 ggcaacttct tactattacc cgccctgccg aagcggctga agctcgctgg gaagaagtag
    19921 acatagaagc gcaagagtgg aagattcccg cagcacgcat gaaaatgaac cgcgaccata
    19981 ctgttccatt gtcagatgaa gcaatagctg tgctggagat gatgaaaccg ctaagcggca
    20041 accgagaatt tatctttcct agccgtatca agcccaacca gcctatgaac agtcagactg
    20101 ttaacgcctc actaaaacgc gcaggttttg gtggtgtgct tgtttcgcat ggtctgagat
    20161 caattgccag tacggctctt aatgaacaag gctttccgcc tgatgccatt gaggcagcat
    20221 tggcccatgt ggataagaat gaggttcgtc gtgcttataa ccgcagcgat tacctggaac
    20281 agcgtcgccc gatgatgcaa tggtgggcaa attttgtgat ggcagcggat cgtggaagca
    20341 tgattgaagg tgggataaaa ggaatgaagc tagttgggtg actaaatagc tgcgcggcat
    20401 agtgtcccac tttcagccag cccttgcagg ttaagccacc agacagtaaa cggcgggtgt
    20461 cgcgttatta ggcgcagcta tttagtagta atattaggat tggtgattct gcactccaga
    20521 agttcggtaa tgctgccaac ttact
//
LOCUS       DAJTRD010000072        20538 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTRD010000072 DAJTRD010000000
VERSION     DAJTRD010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20538
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.072"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            75..227
                     /gene="hokA"
                     /locus_tag="OO315_002817"
     CDS             75..227
                     /gene="hokA"
                     /locus_tag="OO315_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5891661.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO315_002818"
     CDS             complement(414..626)
                     /gene="cspA"
                     /locus_tag="OO315_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5891662.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(907..1197)
                     /locus_tag="OO315_002819"
                     /pseudo
     CDS             complement(907..1197)
                     /locus_tag="OO315_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            1631..2341
                     /locus_tag="OO315_002820"
     CDS             1631..2341
                     /locus_tag="OO315_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5891663.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO315_002821"
     CDS             complement(2391..3365)
                     /gene="ghrB"
                     /locus_tag="OO315_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5891664.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3469..4119)
                     /locus_tag="OO315_002822"
     CDS             complement(3469..4119)
                     /locus_tag="OO315_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5891665.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            4282..6615
                     /gene="bisC"
                     /locus_tag="OO315_002823"
     CDS             4282..6615
                     /gene="bisC"
                     /locus_tag="OO315_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR5891666.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6584..7024)
                     /locus_tag="OO315_002824"
     CDS             complement(6584..7024)
                     /locus_tag="OO315_002824"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5891667.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7021..7584)
                     /gene="tag"
                     /locus_tag="OO315_002825"
     CDS             complement(7021..7584)
                     /gene="tag"
                     /locus_tag="OO315_002825"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5891668.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            7742..8440
                     /locus_tag="OO315_002826"
     CDS             7742..8440
                     /locus_tag="OO315_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5891669.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8669..9877
                     /locus_tag="OO315_002827"
     CDS             8669..9877
                     /locus_tag="OO315_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5891670.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10201..11892
                     /gene="eptB"
                     /locus_tag="OO315_002828"
     CDS             10201..11892
                     /gene="eptB"
                     /locus_tag="OO315_002828"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5891671.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            11984..12060
                     /locus_tag="OO315_002829"
     tRNA            11984..12060
                     /locus_tag="OO315_002829"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12018..12020,aa:Pro,seq:cgg)
     gene            12804..14411
                     /gene="dppA"
                     /locus_tag="OO315_002830"
     CDS             12804..14411
                     /gene="dppA"
                     /locus_tag="OO315_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5891672.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            14719..15738
                     /gene="dppB"
                     /locus_tag="OO315_002831"
     CDS             14719..15738
                     /gene="dppB"
                     /locus_tag="OO315_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5891673.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            15748..16650
                     /gene="dppC"
                     /locus_tag="OO315_002832"
     CDS             15748..16650
                     /gene="dppC"
                     /locus_tag="OO315_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5891674.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            16661..17644
                     /gene="dppD"
                     /locus_tag="OO315_002833"
     CDS             16661..17644
                     /gene="dppD"
                     /locus_tag="OO315_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5891675.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            17641..18645
                     /gene="dppF"
                     /locus_tag="OO315_002834"
     CDS             17641..18645
                     /gene="dppF"
                     /locus_tag="OO315_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5891676.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(18675..19946)
                     /locus_tag="OO315_002835"
     CDS             complement(18675..19946)
                     /locus_tag="OO315_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5891677.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            20422..20529
                     /locus_tag="OO315_002836"
     CDS             20422..20529
                     /locus_tag="OO315_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5891678.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT         4922 a         5082 c         5401 g         5133 t
ORIGIN      
        1 agtgaggcta ccctatgcct gaacaccata aggttagcct cttactcgtt ggaaatcaac
       61 acagggggct gggaatgccg cagaaatata gattactttc tttaatagtg atttgtttca
      121 cgcttttatt tttcacctgg atgataagag attcactgtg tgaattgcat attaaacagg
      181 agagttatga gctggcggcg tttttagcct acaaattgaa agagtaagag tcttcgacgg
      241 gaaattattc ccgccttact tacggcgttg cgcattctca ttgcacccat atttattctt
      301 cacaaaaata ataatagatt ttattacgcg atcgattatt tatttcctga aaaaaaataa
      361 aaaaatcccc gccaaatggc agggatctta gattctgtgc ttttaagcag agattacagg
      421 ctggttacgt taccagctgc cgggccttta gcgccgcttt cgatggtgaa ggacactttc
      481 tgaccttcgt ccagagattt gtaaccatcg ttctggatag cagagaagtg tacgaacaca
      541 tctttagagc catcgtcagg agtgatgaag ccgaagcctt tgtcagcgtt gaaccatttt
      601 acgataccag tcattttacc ggacatagtg tattaccttt aataattaag tgtgcctttc
      661 ggcgatatgg cgtgctttac agattttgaa gcgttaaagg aatgtgcact acgaggggta
      721 tcaacgataa ctcttgaagg gacttgcctt actacactgc tttaatggtc tgtacgtcaa
      781 accgttgatg tgcattaagc cactcattgg cgggtgatgc aacaattatt tttcatattt
      841 atgattaatc ggttggaaaa cggtcctgtc atcaggaccg taaacagcaa taaagtggat
      901 aaaagtctat ttcatcaact gcttacttaa tgccgggttg gcttgaatca aacgcatcaa
      961 ttttagttcg gcacttgaag gcttcacgcg tctggattcc cattccttta ccatggcgac
     1021 tgatacgccc aaaacccggg cgaaatcatc gatttttaat cctgtccctt ttcataactg
     1081 ctcaatttcg gtacaggacg ttgttctgtg cgtcagggta attttctgcg tttcatcttt
     1141 aaaaacaatc tgttccaggc tgctcaacag ctcatgcatt ggatctttat attccattga
     1201 aaactcctca aatcacactg cgggatcgtg aattgcatcg aagctcatta agaatagtcg
     1261 ggaaaagcag acacagcggg gtgaagtcag ggcatgctct ggctatgatt cgattcagcc
     1321 tgtcatttag cgtgacttat agccacctaa cgatctgata aatgccgata tatctcttat
     1381 cactcctgtt ttgtaaagat ttcgcaaatc taaccttctg cgaaggcgta aatctctggc
     1441 tacaaaaaag gcaacggcac tatcattttt taacaatgaa acaacaaatt aaccttgttt
     1501 cattttctcc atgattttac caggaagatt acgtagaaag ggtatcctgc aagcctgtcc
     1561 tggactatcc ttgtcacgtc agacacgcgt gtgtcgttgt gcgctttttt tgggtgaaag
     1621 gagtaagaaa atggcgacag gaaagtcctg ctctcgctgg tttgcgcctc ttgcggcgtt
     1681 attaatggta gttagcctga gtgggtgttt tgataaagaa ggcgatcagc gtaaagcgtt
     1741 tatcgacttc ctgcaaaata cagtgatgcg tagcggtgaa cgtctaccaa ccctgactgc
     1801 cgatcagaaa aaacagtttg gtccttttgt ctctgattac gcgattctgt atggttattc
     1861 tcagcaggtg aatcaggcga tggattccgg tctgcgcccg gtcgtagaca gcgttaacgc
     1921 aattcgcgta ccgcaggatt acgttacgca aagcggtccg ctgcgtgaaa tgaatggttc
     1981 gctgggtgtg ctggcgcaac aactgcaaaa tgcgaagttg caggccgatg ctgcgcactc
     2041 tgcgctaaaa cagagcgatg acctgaaacc ggtatttgat caggcattca ccaaagtggt
     2101 aacgacgcca gctgatgcat tgcaaccgtt gattccggcg gcacaaactt tcacgcaaca
     2161 actggtgatg gttggagact acattgctca gcaaggtact caggtgagct ttgtcgcaaa
     2221 tggcatccag ttcccgactt cacagcaggc aagtgaatat aacaaactga ttgcgccatt
     2281 accggcacaa catcaggcgt ttaatcaggc ctggactaca gcagttactg caacccaata
     2341 aagagtaaaa gcccgagcga ttatcgggct ttacttcacg cagtcgcagc ttagtccgcg
     2401 acgtgaggat tcacacagtt cttctcaacc tttccttgta acgcatcaat caaattatcc
     2461 acggcacagg cggccatgcc ataacgcgtc tcatgggtgg cagatccaat atgcggtact
     2521 gcgacgacgt tggccattga gagcaacggc gaatctacgg acagtggctc ttgttcgaag
     2581 acatccagcc cggcggcgtg aatttcgcct ttctgcaatg ctgcgatcag tgcattttcg
     2641 tcaaccaccg ggccacgtcc ggcattaatg aaaatggcgg aggatttcat tttggcgaat
     2701 tgttctgcgc caaacagatg atgcgtctca tcagttaacg gcaggatcag gcaaacgaaa
     2761 tctgactctt gtaacagagt atccaaatcg cagtagcggg cgttgaagcg ttcttctgct
     2821 tctttatggt ggcggcgcgc gttatagagg atgggcatgt tgaagccaaa gtgcacacgt
     2881 tgtgccagcg ccatgccgat ccgtcccatc ccgacaatgc ccagtgtttt atggtgaacg
     2941 tcagtgccgt accagtccgg gcctatgctc gcggtccatt cgcctgcttt tacccgttct
     3001 gctacctcca caacccgacg agcggtagac aacaccagcg ccatcagcgt atcggcgacg
     3061 gtttctgtta atacggttgg cgtgtgcatc agcagaattt ttcgggcggt aagcgcatcg
     3121 acatcaaaat tgtcatagcc gacggagatc gttgatgtgg cacgcagttt cggcattttt
     3181 tccagcaatg cggcatctac attctcgttt gaacccagta aaccttcagc ttctgcaaaa
     3241 attgctgcat tttgaacgac ggtttgtggg ctgaggtttg ccacctggtg aacggtgaaa
     3301 tgcgcttgca ggcgttgcag taaatcatca ggtaaggctt tgtagaggat aacggacggc
     3361 ttcatgcttc tctccattca ctgataaggc gatcacctta gcctgaaata tgatgaagga
     3421 aaactgaaga atgccaggtg atttctgcat cacctggcat gaaagggatt acagcgggct
     3481 taaggtaatt tctacacggc ggttttgcgc cttaccttct gcggtgctgt tgctggcgat
     3541 tgggttagcc gggccaaggc cctgagtacg gatgcggctg gcgtccacgc cctgggtgat
     3601 caacgcgctg gcaacggaat ccgcacgctg ctgggagaga cgcatgttca ggtcgtgacc
     3661 ccccgtgctg tcggtataac caatcacgtt aaccgccgtt ttcggatact ctttcagcac
     3721 cattgccacg ccggtcaggg tgttagcgcc tgccggtttc aggggagcgc tgctgctgtc
     3781 gaaggtcaca ttgttcggca tattgaggat aatgttatcc ccgctgcggg ttacgctaac
     3841 accagtgccg cgcattttgt cacgcagctt cgcttcctgc acatccatgt aataacccac
     3901 gccgccgccc agagctgcgc ctgctgctgc gccaatcagc gcgcctttac cgcgatcttt
     3961 cttcgaagaa gagagcgcac caatacccgc gcccacgaga gagcccagac ctgcgccgat
     4021 agcagatttg cctgcttcgc gttcgccggt gtaagggtta gttgtgcagc cagataccgc
     4081 cagagcgcca ctcactacgg cggcaagtaa gataaacacg tttcttcatt gttaatcctt
     4141 aataaccttt ttattctttg tcacgggttc cgtggcggga gattatgccg cgtgaacatg
     4201 aagattattc ctgggaatac tcggaaattt gtaagtaata tttaactgct caatacatct
     4261 aacctttcag gagtcttcgg tttggccaac tcatcctcac gatattccgt tctgactgcc
     4321 gcccactggg ggcccatgct ggttgaaacc gacggcgaaa ccgtgtttag ctcgcgtggc
     4381 gcgttagcca caggaatgga aaactccttg cagagcgcgg ttcgcgacca ggttcacagc
     4441 aatacgcggg tacgatttcc aatggtgcgc aaaggctttc tcgcgtcacc ggaaaacccg
     4501 caaggcattc gtgggcagga tgaatttgtt cgcgtgagtt gggatgaggc gctggatctt
     4561 attcaccaac aacataaacg cattcgtgag gcttatggtc cggcatcgat ttttgctggt
     4621 tcctacggct ggcgttcaaa cggcgtgctg cataaggcct cgacattatt acaacgctat
     4681 atggcgctgg caggcggtta taccgggcat ctgggggatt attcgaccgg tgcggcacag
     4741 gcgatcatgc cgtatgtcgt aggtggcagc gaagtttatc aacagcaaac cagttggccg
     4801 ctggtgctgg aacatagcga tgtcgtggtg ttgtggagtg ctaacccact caatacgctg
     4861 aaaattgcgt ggaatgcatc cgatgagcag gggctttctt acttttccgc actgcgtgac
     4921 agtgggaaaa agctgatctg cattgatcca atgcgatcgg aaaccgtcga tttctttggc
     4981 gataaaatgg agtgggtggc accgcacatg ggcaccgatg ttgcgctgat gctggggatc
     5041 gcccatacgc tggtggaaaa tggttggcac gacgaagcgt ttctggcgcg ttgcaccaca
     5101 ggttatgccg tcttcgcctc ttatttgctg ggcgagagtg acggaatagc gaaaaacgcc
     5161 gaatgggcgg cagagatttg tggtgttggc gcagcgaaaa tccgcgagct ggcggctatt
     5221 ttccaccaaa ataccaccat gctgatggca ggctggggaa tgcagcgcca acagtttggt
     5281 gagcaaaaac actggatgat cgtcacgctg gcagcaatgt tggggcaaat cggcacaccc
     5341 ggcggcggtt ttggtctttc ttaccatttt gccaatggtg gtaaccccac gcggcgttct
     5401 gcggtgctct cttccatgca gggcagcttg ccgggtggct gcgatgcggt ggataaaatc
     5461 cctgttgccc gcattgttga agcactggaa aaccctggtg gcgcatatca acacaacggt
     5521 atgaaccgac atttcccgga tattcgtttt atctggtggg cgggcggtgc caactttact
     5581 catcatcagg ataccaatcg tctgatccgt gcctggcaaa aaccggagct ggtggtgatc
     5641 tctgaatgct tctggacggc ggcggcgaaa cacgcggata ttgttctgcc tgcgactacc
     5701 tcttttgagc gtaatgatct caccatgacc ggtgattaca gtaatcagca tctggtgccg
     5761 atgaagcaag tggtgccgcc acgctatgaa gcgcgtaatg attttgatgt ttttgccgag
     5821 ttgagtgaac gttgggagaa gggcggctat gcgcggttta cggaaggaaa aagtcagctg
     5881 caatggctgg aaacgtttta taacgttgcc cggcagcgcg gggcaagcca gcaggttgaa
     5941 ttgccgccat ttgctgagtt ctggcaagcc aaccagttaa ttgagatgcc ggaaaacccg
     6001 gacagcgagc ggtttattcg cttcgctgat ttttgccgcg atccgctggc gcatccgtta
     6061 aaaaccgcca gcggcaagat tgaaatcttc tcacagcgta ttgccgatta cggttacccg
     6121 gattgccctg ggcatccaat gtggctggag ccggacgaat ggcagggcaa tgccgaacca
     6181 gaacagttgc aggtactttc tgcccatccg gcgcaccgcc tgcacagcca gctgaattac
     6241 agttctctgc gcgaattgta cgcggtagca aaccgtgagc ctgtcaccat tcatcctgac
     6301 gatgctcagg cgcgcggcat aaccgaaggg gatatggtgc gggtgtggaa ctcccgtggg
     6361 cagatccttg ccggagcggt cattagcgag ggaattaaac ctggcgtgat ttgcattcac
     6421 gaaggggcat ggccggatct ggatttaacc gctgacggta tttgtaaaaa cggcgcagtg
     6481 aacgtgctga ccaaagatct ccccagctcg cggctgggga atggctgtgc gggtaatacg
     6541 acgctggcat ggctggaaaa atacaacggt ccggaactga cacttacagc gtttgaacca
     6601 ccggccagct cataatccag gtgggtagtt gggtttcatc ctgccatgcg caatcgacaa
     6661 tgtgaaaacc ctgcgcacgg taaaaatcta tcgccggttg atttttttga taaacctcca
     6721 gcatcagatg gggatagcgc tgctgcacat actgcatcag cgccttacca ataccgcgtc
     6781 tgacggcctt cggtgcgaca aacattgctg ccagaaatcg gccttccata atgctgacaa
     6841 aaccgagaag cttaccgtcc tcttcccaga cccagttttg cgcgttggca agataggcat
     6901 cccgcaccag cggaatgcag tcacgccagt aattcgcttt tataaaggga tgcccccagg
     6961 ttgtactttc cagccacagt tcgaggatcg cggggagttc tgaacgttgc gcttcccgaa
     7021 tcatggttta tttcccagat agcaacagca gccaaccaca tgatcattca ccagcccaca
     7081 tgcctgcata aaggagtaac agattgtggt gccgacaaac ttaaaaccac gttttttcag
     7141 tgccttagat agggcgtcgg aggcggacgt agatgtggga atttcgctca acgttgtggc
     7201 ttgtgtcacc tgtggctgat gatttacaaa cgaccagaca aagtcgggaa acggttcgcc
     7261 gttctgttcc atttgcaggt acgcccgcgc attaccaata attgcctgaa ttttccctcg
     7321 atggcggata atcccggcgt cctgtaccag tctttcgaca tcctcttcct gcattgctgc
     7381 gaccttcacc ggatcgaact gatgaaagta ggcgcgatag ttttcgcgtt ttttgaggac
     7441 ggtgatccac gataacccag cctgctgccc ttcaaagcag atcatttcga acagtttttt
     7501 actgtcagtt tcaggcacgc cccactcatt atcatggtag gcaatataaa gcgggccctg
     7561 actcacccag ccgcaacgtt ccatactttc cctcgctatg tacccgatcg taaaaatttc
     7621 agccgctttt cttgacgcat cggtgaaaaa gtcaatattt aatacagggc tactatgccc
     7681 gatatccttc atacaatgac aataatattg ccgaattcgg ctcttccctg gagtcgtgtt
     7741 aatgatcata aaaaaaagcg gtggtcgctg gcagctaagc ctgctggcga gcgtggtaat
     7801 cagtgccttt tttctcaaca cagcttacgc ctggcaacaa gaatatatcg ttgatacgca
     7861 acccggacat tccacagagc gttacacctg ggatagtgat catcaacctg attacaacga
     7921 tattttgtcg caacgtattc aaagtagcca aagggcgctg ggactggaag tcaatctggc
     7981 ggaagaaact cctgtggatg tgaccagcag tatgagtatg ggctggaatt ttcctttata
     8041 tgaacaggtt acaaccggcc cggtcgcggc attacattac gatggcacaa ccacctcgat
     8101 gtataacgag tttggcgaca gtactaccac gctgaccgat ccgttatggc atgccagcgt
     8161 gagtagctta ggctggcgtg ttgactcccg gcttggcgat ctccgaccct gggcgcaaat
     8221 cagctataac cagcaatttg gcgagaatat ctggaaggcg caatcaggcc tgagccggat
     8281 gacggcgaca aaccagaacg gcaactggct ggatgtcacc gtaggcgctg atatgttgct
     8341 caatcaaaat attgccgcct atgctgcgct aactcaggca gaaaatacca ctaataatag
     8401 cgactatctg tatacgatgg gggttagcgc cagattttaa cgtaacagtc acaattgaaa
     8461 ccattgaata acaacagttg tggcgatagt gggtgctaac ttaccaaata ataaatttgg
     8521 tgaataattg tcgcgttatt cattcctgaa ctaaggcatt tcattccgtt ctgatggcat
     8581 ttcatgccgt ttttccccaa gcataaaatg cacttcgtta tggttgtcgg cagagatttt
     8641 tcctttttat tactgcagga atactgccat gacaccttca aattatcagc gtacccgctg
     8701 gctgacactc atcggtacta tcattaccca gtttgcgctg gggtcggttt atacccggag
     8761 cctgtttaat ggcgcgcttt ctgccaagct ggatgcgccg gtaagccagg tcgctttctc
     8821 tttcggcttg ttaagtctgg ggctggcaat ttcgtcttct gttgcgggca aattgcagga
     8881 acgttttggc gttaaacgcg tcaccgtggc ttccggcatt ttgctgggat taggcttctt
     8941 cctgaccgcg cattccaaca acctgatgat gctgtggtta agcgccggtg tgctggtcgg
     9001 tctggcggat ggcgcgggtt acctgctgac gctctctaac tgcgtgaagt ggttcccgga
     9061 gcgtaaaggt ctgatctccg cgttcgctat cggttcttat ggtctgggta gcctgggttt
     9121 caaatttatc gacacgcagc tgctcgaaac ggtgggtctg gaaaaaacct ttgtgatttg
     9181 gggagcgatt gcgctggtga tgattgtttt cggcgcaacg ttaatgaaag acgcaccaaa
     9241 acaggaagtg aaaaccagca atggtgtggt ggagaaagat tacacgctgg cagagtcgat
     9301 gcgtaaaccg cagtactgga tgttagcggt aatgttcctg actgcctgca tgagcggcct
     9361 gtacgtgatt ggggtagcga aagatatcgc ccaaagtctg gcacaccttg atgtggtttc
     9421 cgcagccaat gcagtaacgg ttatttccat cgctaacctt tcaggtcgtc tggtgctggg
     9481 cattctgtct gacaaaattg cccgtatccg tgttatcacc attggtcagg tgatttcgct
     9541 ggtgggtatg gcggccctgc tgtttgcacc attaaatgca gtgacgttct ttgcagcgat
     9601 tgcctgcgtg gcatttaact ttggcggcac tattaccgtc tttccatcac tggtcagtga
     9661 gttcttcggc ctcaataacc tggcgaaaaa ctacggtgtg atttacctcg gtttcggtat
     9721 cggtagcatt tttggttcga ttatcgcctc actgtttggc ggtttctatg tgaccttcta
     9781 cgtgattttc gccctgctga ttctgtcatt ggcgctttct acgacgattc gtcagccaga
     9841 gcagaaaatg ttgcgtgagg cgcatggctc cctttaatgc tcagccgcga gctactgggc
     9901 ttcagcttcc gtgtgcatca aacgcatcaa cgaaatgtat aaacaatgac tcgtaaaatt
     9961 tgggccagtt aactggccct ttttactgct ttttgtaacg ctaaagtctc ttttcaaact
    10021 tgcatttttg taaatttgtg cttcatgcac actctttccc cacacttttt cgctttgctg
    10081 tggtctactt attcgcgcgt gtagatttta cttatctgac tacctccgca cttttcccct
    10141 gccgggcctg aaaagccgct aagcagggtg ttatcacctg tttgtccagg gtttgtttgc
    10201 atgagataca tcaaatcgat tacacagcag aagctgagct ttttgcttgc aatctatatt
    10261 ggccttttta tgaatggcgc ggttttttat cgccgcttcg gcagctatgc gcacgatttt
    10321 accgtctgga aaggcgtttc tgctgttgtt gaactggccg ccaccgtact ggtgaccttc
    10381 tttttactac gtcttctatc gctgtttggc cgccgcagct ggcgtattct ggcgtcgctg
    10441 gtggtgctct tttccgcagg tgccagctat tacatgacct tccttaatgt ggtcattggt
    10501 tatggcatca tcgcttccgt catgaccacc gatatcgacc tgtcaaaaga agttgttggt
    10561 ctgaacttta ttctctggtt aatcgccgtt agtgcattgc ctcttatcct tatctggaat
    10621 aaccgctgtc gctacacctt gctccgacaa ctgcgaaccc cagggcagcg tattcgcagc
    10681 ctggtggtcg tcgtactggc gggtattatg gtttgggcac cgattcgttt gctggatatc
    10741 cagcagaaga aagtggagag ggcgaccggc gttgatttgc cgagttatgg cggtgtcgta
    10801 gcgaactctt atctgccatc aaactggctt tctgcgttgg ggctgtatgc ctgggcgcgg
    10861 gtcgatgaat cttccgataa taattcattg cttaatccgg cgaagaagtt tacataccag
    10921 gctccacaaa acgttgatga tacttatgtc gtgtttatca tcggtgaaac cacgcgttgg
    10981 gaccatatgg gtattttcgg ctatgagcgt aataccacgc cgaaactggc ccaggagaaa
    11041 aatctggcgg cgttccgtgg ttactcctgt gataccgcaa ccaaactctc actgcgttgc
    11101 atgtttgtac gtcagggggg cgcggaagat aatccgcagc gcacattaaa agaacagaac
    11161 attttcgcgg ttctgaagca gttaggattc agttctgacc tctacgctat gcagagcgaa
    11221 atgtggttct acagcaacac gatggcagac aacattgctt atcgtgagca gattggtgcg
    11281 gagccacgta atcgtggcaa gccggtagat gatatgttgc tggtagacga aatgcagcaa
    11341 tcgctagggc gcaacccgga tggtaagcat ctgatcattc tgcataccaa aggttcgcat
    11401 tttaactaca cccagcgtta tccgcgtagc ttcgcgcagt ggaagccgga atgtattggt
    11461 gttgatagcg gctgtaccaa agcgcagatg atcaactcct atgacaactc ggtgacctat
    11521 gtggatcact ttatctccag cgtgattgat caggttcgcg ataagaaagc gattgtgttc
    11581 tacgcagctg accacggtga gtcaattaat gaacgcgagc acctgcacgg cacgccgcgt
    11641 gaactggcac cgccggagca gttccgcgta ccgatgatgg tctggatgtc agataaatat
    11701 ctggaaaatc cggccaatgc gcaggcgttt gcgcagctga aaaaagaagc cgacatgaaa
    11761 gtgccacgcc gtcacgtaga gctgtacgac accatcatgg gttgtcttgg ctatacttca
    11821 ccggatggtg gaattaacga aaacaacaac tggtgtcaca tcccgcagac aaaagtggca
    11881 gcggctaact aagcggcctg ctgactttct cgccgatcaa aaggcatttt gctattaagg
    11941 gattgacgag ggcgtatctg cgcagtaaga tgcgccccgc attcggtgat tggcgcagcc
    12001 tggtagcgca cttcgttcgg gacgaagggg tcggaggttc gaatcctcta tcaccgacca
    12061 aattcgaaaa gcctgctcaa cgagcaggtt tttttgcatt tgtagctcat gaggatgaga
    12121 acctccgggg gcagggaggc tcgactcgac gtaggcctga taagacgcgc cagcgtcgca
    12181 tcaggcaacg gcaccgcact tattgtcgga tgcggcgcga ccgccttatt cgaccaacac
    12241 accacacatt ttcctgccaa ctttgcgccc catatctcac tatccattaa cggatttgtg
    12301 acagaaacca ttgtcttttt ttatatttgc ttaaatcttt tttgcgttac ttatggctgg
    12361 cgctggcatt ttccgctgtg cgttttagcg ttcatggtat taatcgctca gatatccacc
    12421 ttttcctcag atttttttca ttaaatgcat aaaacttaat caccaaatgt tgctaaatat
    12481 taagctgatt gttctggtta tgttggagta gcacaaattt ctctgctgca aatagctgtt
    12541 tgaagttttt ttaatctttg tttgtggatt ctcaccgttg gtgtaaatcc cggttggctg
    12601 tattgacgtt ttcacattct gttgacagat tgtaggtcac gaggggcatt ttatggagga
    12661 tccgcactgt tacactgatg ttaattagta cggcatcccc acctcataac gttgacccga
    12721 ccgggcaaaa aacaaaaaag gtcaggcagc gacaacccac tgcaaagggt taaaacaaca
    12781 aacatcacaa ttggagcaga ataatgcgta tttccttgaa aaagtcaggg atgctgaagc
    12841 ttggtctcag cctggtggct atgaccgtcg cagcaagtgt tcaggctaaa actctggttt
    12901 attgctcaga aggatctccg gaagggttta acccgcagct gtttacctcc ggcaccacct
    12961 atgacgcctc ttccgtcccg ctttataacc gcctggttga atttaaaatc ggcaccaccg
    13021 aagtgatccc gggcctcgct gaaaagtggg aagtcagcga agacggtaaa acctatacct
    13081 tccatctgcg taaaggtgtg aagtggcacg acaataaaga attcaaaccg acgcgcgaaa
    13141 tgaacgccga tgacgtggtg ttctcgttcg atcgtcagaa aaacgcgcaa aacccgtacc
    13201 ataaagtttc tggcggcagc tacgaatact tcgaaggtat gggcttgccg gagctgatca
    13261 gcgaagtgaa aaaggtggac gataacaccg ttcagtttgt gctgactcga ccggaagcgc
    13321 cgttcctcgc tgacctggca atggacttcg cctctattct gtcaaaagaa tatgctgacg
    13381 cgatgatgaa agccggtaca ccggaaaaac tggacctcaa cccaatcgga accggtccgt
    13441 tccagttaca gcagtaccaa aaagattccc gtatccgcta caaagcgttt gatggctact
    13501 ggggcaccaa accgcagatc gatacgctgg tcttctctat tacccctgat gcttccgtgc
    13561 gttacgcgaa attgcagaag aatgaatgcc aggtgatgcc gtacccgaac ccggcagaca
    13621 tcgctcgcat gaagcaggat aaatccatca acctgatgga aatgccgggg ctgaacgtcg
    13681 gctacctctc gtataacgtg cagaaaaaac cgctggatga cgtgaaagtt cgccaagcac
    13741 taacctacgc ggtgaacaaa gacgcgatca tcaaagcggt ttatcagggc gcgggtgtat
    13801 cagcgaaaaa cctgatcccg ccaaccatgt ggggctataa cgacgacgtt caggattaca
    13861 cctacgatcc tgaaaaagcg aaagccttgc tgaaagaagc gggtctggaa aaaggtttct
    13921 ccatcgacct gtgggcgatg ccggtacaac gtccgtataa cccgaacgct cgccgcatgg
    13981 cggagatgat tcaggcagac tgggcgaaag tgggcgtgca ggccaaaatc gtcacctacg
    14041 aatggggtga gtacctcaag cgtgcgaaag atggcgaaca tcagacggta atgatgggct
    14101 ggactggcga taacggggac ccggataact tcttcgccac cctgttcagc tgcgccgcct
    14161 ctgaacaagg ttctaactac tcaaaatggt gctacaaacc gtttgaagat ctgattcaac
    14221 cggcgcgtgc taccgacgac cataacaaac gcgttgaact gtacaagcaa gcgcaggtgg
    14281 tgatgcacga tcaggctccg gcactgatca tcgctcactc caccgtgttt gaaccggtac
    14341 gcaaagaagt caaaggctat gtggttgatc cattaggcaa acatcacttc gaaaacgtct
    14401 ctatcgaata attaaaagcc atacaagact gatggcaaag gcaaaactgc ctgatgtgct
    14461 ccgcttatca ggcctacgaa aattctgcaa tgtattgaat ttgcacgatt ttgtaggccg
    14521 gataaggcgt taacgccgca tccggcataa acaaagcgca ctttgtcaac aatctgtata
    14581 cccggtggcg ctgtgcctct gcgtgcagcg ccatccggca gcaatacttc attctctgtc
    14641 cgacaagggc gggaattgat ttgtgagcaa tacagacacg cagttccagg ctgcgagtca
    14701 ctatagagaa tccgggttat gttgcagttt attctccgac gtttgggact cgtcatcccc
    14761 acgtttatcg gtattaccct tctcacattt gcctttgtcc acatgatccc gggcgatccg
    14821 gtgatgatca tggcaggcga acgtgggatc tcccctgagc gtcacgtgca gctgctggct
    14881 gaactcggct tagataaacc gatgtggcag cagtatctcc attacatttg gggcgtaatg
    14941 cacggcgatc taggcatttc aatgaaaagc cgaattccag tatgggaaga gttcgtgccg
    15001 cgctttcagg ccacgctgga acttggcgtc tgcgcgatga tttttgctac ggcagtcggt
    15061 attccggttg gtgtgctggc tgcggttaaa cgcggttcca ttttcgatca cactgcagtt
    15121 ggcctggcgc tgaccggcta ctcgatgccg atcttctggt ggggcatgat gctgatcatg
    15181 ctggtttcgg tgcactggaa cctgacgccc gtctccggtc gcgtgagcga tatggtgttc
    15241 ctcgatgact ccaatccgtt aaccggtttt atgctaatcg acaccgccat ctggggtgaa
    15301 gacggcaact ttatcgatgc cgtcgcccat atgatcttgc ccgccattgt gctgggtact
    15361 attccgctag cggtcattgt gcgtatgaca cgctcctcga tgctggaagt gctgggcgag
    15421 gattacatcc gcaccgcgcg cgccaaaggg ctaacccgca tgcgggtgat tatcgtccat
    15481 gcgctgcgta acgcgatgct gccggtagtg accgttatcg gcctccaggt gggaacattg
    15541 ctggcggggg cgattctgac cgaaaccatc ttctcgtggc ccggtctggg gcgctggttg
    15601 attgacgcac tgcaacgccg cgactatccg gtagtgcagg gcggcgtatt gctggtggcg
    15661 acgatgatta tcctcgtcaa cttgctggtc gatctgctgt acggcgtggt gaacccgcgt
    15721 attcgtcata agaagtaagg ggacatcatg tcacaggtta ctgaaaataa agtgattagc
    15781 gcaccggtgc cgatgacccc gttacaggag ttctggcact attttaaacg caacaaaggc
    15841 gcggtcgtcg ggctggttta cgtcgccatc gtgctgttca tcgcgatctt tgccaactgg
    15901 attgcgcctt ataacccggc ggatcagttc cgcgatgcgc tgctcgcccc gccagcctgg
    15961 caggaaggcg gcagtatggc acacttgctg ggcaccgatg acgtcggtcg tgatgtgctg
    16021 tcgcggctga tgtacggcgc gcgtttatcg ctgctggttg gctgtctggt ggtggtgttg
    16081 tcgctgatta tgggcgttat tctcggcctg atcgccggtt acttcggcgg cctggttgat
    16141 aacattatca tgcgcgtggt cgatatcatg ctggcgctgc caagtctgct gttggcactg
    16201 gtgctggtgg cgattttcgg cccgtcgatt ggtaacgccg cgctggcgct gaccttcgtt
    16261 gccttgccgc actatgtgcg cttaacccgc gccgccgtgc tggtggaggt gaatcgcgat
    16321 tacgtcaccg cctctcgcgt ggcaggtgcc ggggcgatgc gccagatgtt tattaacatc
    16381 ttcccgaact gccttgcgcc gctgattgtt caggcgtcgc tcggtttctc taacgccatt
    16441 ctcgatatgg ctgctcttgg tttcctcggc atgggggcac agccgccaac gcctgagtgg
    16501 ggcaccatgc tctccgacgt gttgcagttc gcgcaaagcg cctggtgggt cgtgaccttc
    16561 cctggtctgg cgatcctgtt gacggtgctg gcatttaacc tgatgggtga cggtctgcgt
    16621 gacgcgctcg atcccaaact aaagcagtaa gaggttcgag atggcgttat taaatgtaga
    16681 taaattatcg gtgcatttcg gcgacgaaag cgcaccgttc cgcgccgtag accgcatcag
    16741 ctacagcgta aaacagggcg aagtggtcgg gattgtgggt gagtccggct ccggtaagtc
    16801 ggtcagttca ctggcgatta tggggctgat tgattatccg ggccgcgtaa tggcagaaaa
    16861 actggagttt aacggccagg atttgcagcg tatctcagaa aaagagcgcc gcaacctggt
    16921 gggtgccgaa gtggcgatga tcttccagga cccgatgacc agccttaacc cgtgctacac
    16981 cgtgggtttc cagattatgg aagcgattaa agtgcatcag ggcggcaaca aaagtacccg
    17041 ccgtcagcgg gcgattgacc tgctgaatca ggtcggtatt cccgatccgg cttcgcgtct
    17101 ggatgtttac ccgcatcagc tttccggcgg catgagccag cgcgtgatga tcgccatggc
    17161 gattgcctgt cggccaaaac tgctgattgc cgatgaaccg accaccgcgc tggacgtgac
    17221 cattcaggcg caaatcatcg aactactgct ggagctacag cagaaagaga acatggcgct
    17281 ggtgttaatt acccatgacc tggcgctggt ggcggaagct gcgcataaaa tcattgtgat
    17341 gtatgcaggt caggtagtag aaacgggcga tgcacacgcc atcttccatg cgccgcgtca
    17401 cccgtatact caggcattgc tgcgtgcgct gccagaattt gctcaggaca aagaacgtct
    17461 ggcgtcgttg ccaggtgttg ttcccggcaa gtatgaccgc ccgaacggct gcctgcttaa
    17521 cccgcgctgc ccctatgcca ctgacagatg tcgcgctgaa gaaccggcgc tgaatatgct
    17581 cgctgatggg cgtcagtcca aatgccatta cccacttgat gatgccggga ggcccacact
    17641 atgagtacgc aagaggccac ctcgcaacaa ccgctgttgc aggctatcga cctgaaaaaa
    17701 cattatccgg tgaagaaagg catgttcgtg ccggaacgtc tggtgaaggc gctggacggc
    17761 gtttcgttta accttgaacg tggcaaaacg ctggcagtag tgggcgaatc tggctgcggt
    17821 aaatcgaccc tcggtcggtt gctgacgatg atcgaaacgc ccaccggcgg cgagctgtat
    17881 tatcaggggc aggatctgct caagcacgat ccgcaggcgc agaagctgcg tcggcagaaa
    17941 atccagatcg tcttccagaa tccctacggt tcgctgaatc cgcgtaagaa agttgggcaa
    18001 attctcgaag agccgctgct tattaacacc agcttaagca aagaacagcg tcgggaaaaa
    18061 gccctgtcga tgatggcaaa agtcggcctg aaaaccgagc actatgaccg ctatccgcac
    18121 atgttttccg gcggacaacg tcagcgtatc gctattgccc gtggtctgat gctcgacccg
    18181 gatgtggtga ttgccgatga gccggtttct gcgctggacg tctcagtgcg tgcgcaggtg
    18241 ctgaatctga tgatggattt gcagcaggag ttggggcttt cttatgtctt tatctcccac
    18301 gacctgtcgg ttgtggagca cattgctgat gaagtgatgg tgatgtacct aggccgctgc
    18361 gtggagaaag gaacgaagga ccaaatcttc aataacccgc gccatccgta cacccaggcg
    18421 ctgctctccg cgacgccacg cctgaacccg gacgatcgtc gtgaacgcat caaactcacc
    18481 ggtgaactgc caagcccgct gaatcctccg ccgggttgcg ccttcaacgc ccgctgtcgt
    18541 cggcgcttcg gcccctgcac ccagttgcag ccgcagctaa aagactacgg aggtcaactg
    18601 gtagcttgtt ttgctgttga tcaggatgaa aatccgcagc gttaactcac aaaaccgggg
    18661 ccatgcgctc cggtttactc aatcagcttc aacagtggcg acaagcatag caaaatgccg
    18721 taaagcagaa tcagccaggc tttaaatccg cgaagttttt ccagttgtgc gactttatag
    18781 atcaggaaaa acggaatgag gcacgacaca atgccatata acgggcttcc caactgaaag
    18841 aacaccagca ctgatacacg aaacgaaacc caaatcgtca acgtaatgac gataaaagcg
    18901 cagatcgcca gagtcagcat gcgtgagtta attttcttgg tatcaataat tcggcttaac
    18961 agattgagaa taatgccttt aatagcctcg tggaaaccga gataaatgcc aaagaatgcg
    19021 gtcagtacgg caaagatatt cagcaccgta gaggtgatat gaatgatatg tccagggatc
    19081 acctgcgcgg ccagtgccag tgccgagata ttttgttcaa aggcagaaac ggcttcttcg
    19141 tggctaattg agaaggtaaa cgaaaaggca aaaaacagga tcaccgcgat gagcgtgata
    19201 taactaattc ggtgggtacg cagcgcgagc cgggttgcca gtactttatc tgcttcccgt
    19261 ttacgatagg caatattcat tgggttaagt acctgaataa ataccgcaga aaagaagcaa
    19321 aatggaatgg taagcaggac atcgcggaaa aagacggagg cttgcgggaa ggcggttata
    19381 ttggcgaaat tccagtgcgg gatcatcgca aaaccgaaca cgacaataat ccctactttg
    19441 accaccacca ttggcccaga aatcttaaac agtaatcgtt caccaccaga cgcaatcgct
    19501 atcagtacgg cgaaaatagc gactttataa agtagagatt gtgaaagatc ggcatcggtt
    19561 aaaccgaagg ttttcaggta cgaggcgctg tcgaaaacca cggagagaga gtagataaaa
    19621 atcccgtgga taatcatcaa aaagtagata accccgagga aaattcccca gttcttcccc
    19681 aggtaatgac tgataatatc ggtgtagtca ttacaggaat cgctttcaga aagggttttt
    19741 aaataaatgt cctgcactac ccaggtgaca ggataagcaa tgatcgctgc ggtaataaat
    19801 acccaaattc ccttcaagcc aatttgtact ggcatcagca cggttccggc accaatcgcc
    19861 atgcctatgc ataataaaac ccagccgaag tcgtagcgtg taaacggcaa tttctgattg
    19921 tgtttcgata gtgtgttgtg ctgcataaag tcctgtccag aaagaagtcg gcctcgcaaa
    19981 aatgaataaa cccagtttct cttgcgcaac aggtggagaa gtcgaatacg cagtatgtaa
    20041 aaaatgaggc aacagattca ctggggaggt cgcgaggagg cgcaattcta caaaattgcg
    20101 catttcacta ccagtctgga gtcagcccgg atatgaactg caactattgc aaataaagaa
    20161 attaagctgg aaggcatcgc gcaatgagga agattgccgg gtgctgacag taaaaagcaa
    20221 tcgtagaatc agcgtcaagc cggaaggatt ccggtgaggc gcaatgttgc gggggcttaa
    20281 tccctggtgg cattggttgc tggagagaga aaacccccgc acgttgcagg tatgtacctg
    20341 acaacataac gggggctaat cttgaaccta gaccactcaa gaatagccgc gaaacgttgt
    20401 cattacaaca caggcggcta tatgacgctc gcagagctgg gcatggcctt ctggcatgat
    20461 ttagcggctc cggtcattgc tggcattctt gccagtatga tcgtgaactg gctgaacaag
    20521 cggaagtagc gtgtcatg
//
LOCUS       DAJTRD010000073        20278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTRD010000073 DAJTRD010000000
VERSION     DAJTRD010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.073"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(62..820)
                     /gene="aroD"
                     /locus_tag="OO315_002837"
     CDS             complement(62..820)
                     /gene="aroD"
                     /locus_tag="OO315_002837"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5891679.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLVGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="OO315_002838"
     CDS             complement(851..1717)
                     /gene="ydiB"
                     /locus_tag="OO315_002838"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5891680.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1729..3000)
                     /locus_tag="OO315_002839"
     CDS             complement(1729..3000)
                     /locus_tag="OO315_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5891681.1"
                     /translation="MRTVTKLSFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLSEK
                     FSTDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPN
                     LTLAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSY
                     MLLNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGV
                     SSVLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLK
                     KMVRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEF
                     FPKSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFS
                     RYYRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(3183..4388)
                     /locus_tag="OO315_002840"
     CDS             complement(3183..4388)
                     /locus_tag="OO315_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5891682.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4488..4844)
                     /locus_tag="OO315_002841"
     CDS             complement(4488..4844)
                     /locus_tag="OO315_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5891683.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4980..5087)
                     /gene="rprA"
                     /locus_tag="OO315_002842"
     ncRNA           complement(4980..5087)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO315_002842"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="OO315_002843"
     CDS             complement(5273..6385)
                     /gene="ydiK"
                     /locus_tag="OO315_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5891684.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6774..9830
                     /locus_tag="OO315_002844"
     CDS             6774..9830
                     /locus_tag="OO315_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5891685.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9827..10237
                     /gene="menI"
                     /locus_tag="OO315_002845"
     CDS             9827..10237
                     /gene="menI"
                     /locus_tag="OO315_002845"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5891686.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10337..10525
                     /locus_tag="OO315_002846"
     CDS             10337..10525
                     /locus_tag="OO315_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5891687.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            11073..11441
                     /gene="sufA"
                     /locus_tag="OO315_002847"
     CDS             11073..11441
                     /gene="sufA"
                     /locus_tag="OO315_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5891688.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11450..12937
                     /gene="sufB"
                     /locus_tag="OO315_002848"
     CDS             11450..12937
                     /gene="sufB"
                     /locus_tag="OO315_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5891689.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12947..13693
                     /gene="sufC"
                     /locus_tag="OO315_002849"
     CDS             12947..13693
                     /gene="sufC"
                     /locus_tag="OO315_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5891690.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13668..14939
                     /gene="sufD"
                     /locus_tag="OO315_002850"
     CDS             13668..14939
                     /gene="sufD"
                     /locus_tag="OO315_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5891691.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14936..16156
                     /gene="sufS"
                     /locus_tag="OO315_002851"
     CDS             14936..16156
                     /gene="sufS"
                     /locus_tag="OO315_002851"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5891692.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            16169..16585
                     /gene="sufE"
                     /locus_tag="OO315_002852"
     CDS             16169..16585
                     /gene="sufE"
                     /locus_tag="OO315_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5891693.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16734..17738
                     /gene="ldtE"
                     /locus_tag="OO315_002853"
     CDS             16734..17738
                     /gene="ldtE"
                     /locus_tag="OO315_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5891694.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="OO315_002854"
     CDS             complement(17801..18037)
                     /gene="lpp"
                     /locus_tag="OO315_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5891695.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="OO315_002855"
     CDS             complement(18348..19760)
                     /gene="pykF"
                     /locus_tag="OO315_002855"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5891696.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5314 a         4859 c         5175 g         4930 t
ORIGIN      
        1 acggttaaaa atactttttt tatttcggcg ggggagggta tttccgccga aatattattg
       61 cttatgcctg atgtaaaata gttaataccg tgcgcaaatc atttaccgag atttgccctg
      121 gcgcagaggc tttttttacc gcaccaaaag ttgccgccga gccaaatact tcaccaacca
      181 gacgagaaat tacgccagtt tttgccatcg acatcgtgat gattggacga tcggcatact
      241 gctcctgcat ctccagggtc gcggcaagca acgtcagcac atcgctggta ctttgcggca
      301 tcagcgcaat cttaggaata tcggcgtcga aggactgcat tttgcgcaga cgggcaatga
      361 tttcttcggc ttccggcgtt ttatggaagt catggttgga catgacaact ttcacatcat
      421 gcgcgtgggc gtaggcgacg gtttctttga cctgatcatc gccggtaaat aactccagat
      481 cgatcatatc aaccaggccg ctgtcgatgg ctgcacgatt gagagcaata taagcctcgg
      541 tggaaatcgc ctgctcgccg ccttctttgg cactgcggaa ggtaaacagc agcggttttt
      601 ctggcatggt ttcacggaga atttttgccg ccgccatgac agactccaca ttggagaggt
      661 cggcaaagtg gtccacacgc cattccagaa tatcaaagtc cgcttcacga taggcgagag
      721 cttcggattt cacgcgggcg atatctttcg ccatcagcga gacgatgatt ttaggtgcgc
      781 ccgcaccaat gacgagatct tttacagtta cggttttcat tttttaccct ttctgcacgc
      841 ggtcagcctg tcaggcaccg aaccccatga cctgtttaac atattccaga gggaaatctt
      901 tgccagtcca taacgtgaac tgttcagccc cttgccacaa caacatgccg tatccatcaa
      961 tcgttttgca accagcttgt tgtgcctgct gcaataactt cgtcatatgc gggttataca
     1021 cgcattcagt gaccagcagt cccggatgta acagactgat atcattaacc aatgattcat
     1081 tctcaagggg tttcatacct acttttgtgc cattggttaa aatgtcggcg gaagccaggg
     1141 cttcagcaaa ggcttgctga tcggcgagat cggtatccgt gacgacacaa tcggtgtttt
     1201 cgttaacccg ctgcgcgaag gcgagggctt tatcgaagaa ctcatcccga cggttaaaga
     1261 gtttaatttc ttttaaacct tcaattgccc cctgcgcgcc aatcgccgtt gaggcacctc
     1321 cggcccctaa cagcaccatc gttttgcctt tgatatcaaa accgctctct ttaatggcgc
     1381 gaatatggcc cgtgccgtcg gtgttatagc cacgcagata gccatcatca ttaacgatgg
     1441 tgttgatggc acccaccagt ttggctgctg gcgttaattc atcaacatat tcacacgcca
     1501 gttgtttgtt cggcatcgac acaccagttc cgcgcatttt gagggctttt aatccttcaa
     1561 ttgctccggg aaagctatcg ttatccactt cgaaggccat ataggtaaat ggcaatcccg
     1621 ctttttctaa ggctttattc tgcatttcag gcgataaact gtggcggata ggataagcca
     1681 tcaatccaat caattcgtat tttgcggtaa catccataat taactccttt aacctctatg
     1741 cttaattgtg tttaaccggg tgctaaattt gcgttcgcca aaccgcacat cattctcagg
     1801 aataataaaa acgcggtaat agcggctaaa aacaataatt gcggtaatca gggccagcag
     1861 cgcgaaagta aaatcgagaa caatgatata ttgcaggccg atgttcgaca gataaccggt
     1921 aatcagcgga ataacaaagt tagccagtcc acccatcatc atataaatac tggtgacttt
     1981 ggctttgctt ttggggaaaa actctgacat gaccgaaaca ccgagctgta aaatgccgcc
     2041 agctgctgag aaaccgataa caaaggcgcc ggcattacac accagtggag aagggtacag
     2101 gtagataatg gcagctgtta ttgttgccag cgcagagtta aatacattag cccagatggg
     2161 ccggaccatt tttttcagta gtgcggcaaa aataaagaca cagaccaacg agcccatact
     2221 gtaataagag atggttttta atgcctcagc ttctgacata ccagcaaaag ccatcgcata
     2281 tttgggcatc cacaccacaa tcacataaaa ggtcgagaat gcggctacac cgaacagtac
     2341 cgatgaaaca ccttccagcc agactaaagg tttgctgttc atttgcggta attcattagc
     2401 tacgctggcg tccaccaact ggctggggaa tttgcttttc aacagcatca gcgtgatcag
     2461 tacaaacaga atacccggaa taatcagccc atagccgtac cagatattat tgagcaacat
     2521 atagctcacc agcattgggt agaacatttg cccaaatgac accatcgctt taaccagtat
     2581 gaccgccgaa ccagaggctt tcggaaagca ttccatgagc gcggggtagc cacccgtatc
     2641 cagcgctgag ttagcgatac ctacgcacac tgccagaccg taggcgagag ttaaattcgg
     2701 gcaagcggga ataccaaaga agaatagcag atacattatt actgccatta atatcaccgc
     2761 ccgacgacca aacttatcgg agatcacacc gaagaataaa atactgatca atcgccccaa
     2821 accgataccg gaaattaaat aggcaatgcc cgcgttgtca gtggaaaact tttctgacag
     2881 agatgtcata ttttgggcaa gcgtaataac actaatgccg tgcaggaaat agctgaagta
     2941 aatacaaaga acagccagga taaatggcgt gctgaaagat aatttcgtta ccgtacgtat
     3001 tgttctccga cggtttacat aattactata ctgaagatga ttttccctgg gtacatggca
     3061 taaagtttat gtttccagga atattgcatg tcatagtttg tttttgatcg cgattttcct
     3121 tagttagcgc agaaagagtt atttatccgt aagatcttgc atgacctgaa aagaatattg
     3181 cattacccac ccggagcgac attttccttt agcgagtgat gccgcgtttt tttgcggctg
     3241 cgtaagccga taaacagtgc cagtaaaaaa ccgatggcag tatcgaacca cataatatcg
     3301 gcaatacttc tttgggacag atgagccgta atcaacggaa tagtaaaggt cgcaatactg
     3361 cccgtactgt aatagatccc tgtagcttta cctttagcgt aagggaaacg ttcagccatt
     3421 aacgtcaggc caatttgcac aacacctcca gcagaggtaa aaccaatgac aaaagcaaat
     3481 attatcacca cataaaatgt gggatgcagg cagacggtaa acagagcaat aaatgagata
     3541 aaggtgtaca gcatcagtaa tgttgttggg cgaacggtat tacgaatgag tggagcggta
     3601 ataaatacac aaagcagcga acccacggtg tagatactga gtagtttgat cgacatagtg
     3661 tatgacatgc ctgcaacaaa ttgtccgtac tgtgccagcc actggctaac cagataaaac
     3721 gttgccattg agatatagcc atataaggta taactggcta aatcgataat tgaacagcga
     3781 tgttccgtag agctggtggt tttctttatg acaggtaagc gacgacccgg atggggtggg
     3841 aacgtacaac ggtataaaaa cagagcgtta ataaacataa tgcctgcagc aatcataaag
     3901 gaccaaccga accacagttc agcccacact aacaggctaa tgattagcgg taataaaaat
     3961 tgtccgctgg aaacaaatgc tttaattaaa atattggctg tcccaggtga gcgtggaaaa
     4021 gcttccatca aactgggata agtgcctgca tcgagaaaac tgtttgccat tcccgccaga
     4081 aagccaaaaa cataagcgat aatgatgtta ttggtctgca ggatgtcaaa aaagaaggcc
     4141 atatagcagc acatcccgag catgataaaa gggcggcgac caaagcgatc ggataataat
     4201 cctgcaaaaa gcaagacact taatcgacca atgcccagcg atgagataac tatcgagaca
     4261 cccgcggcat tagtctgcca aagtgtctcc agcgaggcca tattcaggct catcaaaatg
     4321 acgcccatac catgcaccag gtaattaaaa tacaacccaa gtgcggtagg gtaataggga
     4381 tttttcatag gttctactta gcaaaggtgc tttaaaaagt gtagcgatgt tgtgtcagta
     4441 tccattcatt attgttgtaa caacgacaat tcctttctgt ggaaaaatta acaaagccgc
     4501 tccagatcgt gtgcatataa ttcagcggct accgattgat agatcttcca gtcaataaaa
     4561 ttgccatcag ggtagacttg ttgaaacgcg gttaagtcgg ggaaaaaacg catggtgttg
     4621 ttgccgatgc ggttatttat tttctctatg atggcatgaa gatgtttttt tcgttgctga
     4681 cgcatagcca acagttgctc gactacacaa tcaggaatgg cgcatttgcc attttcccac
     4741 tgacgccagc tttcactgtc accggtctgg gcaatccagg tcgcgcattc atcaatagtc
     4801 atggcaaaaa tatggcggag tgcctggagt tcataagcgt tcatcgtgta atggctgcat
     4861 gaaataaaca ataaaatata aataacatta tcagcctgct gacggcttga agagagtcac
     4921 agtatcttgt gcaacattat tggaaagagt gaggggcgag gtagcgaagc ggaaaaatgt
     4981 taaaaaaaag cccatcgtgg gagatgggaa aagactacac acagcaattc gttgtttcac
     5041 tcaggggatt tccatgctta taaatcaata tgttgattta taaccgtgag ctaatagtag
     5101 gcatacggac tttttgcgtc gatcagattc gtctcattag tttaaaaact gtaaagaatt
     5161 tgcgacaggt actaccagct gaatggcggt aagcaggaaa atatggggaa gagcaagcaa
     5221 atatcaatta gcggtgatta acaatcaggc ggcagagccg cctgatgaga aattacttat
     5281 tcggtttctc gatttcgcca agttcttcga gaatttcttc cggttggtcc gtcggtggcg
     5341 ggacttcttc cacccacgcg gcaaacagac gccaggaaac ggctaacaga accggaccaa
     5401 taaacagacc gatcatcccg aaagcaatca aaccaccaat aacgccagag aggatcagga
     5461 tcagcggtaa atcggcaccc atgcgaatta acattgggcg gatgacgtta tccagcgtgc
     5521 caaccacacc gctccacact aacaataccg ttccccaggt ggtatcgccg gtccagtaga
     5581 gccagataat cgccggaatc agtaccggca acgggccaag ctggacaagg caggagagga
     5641 tcattaacac cgttagcaac gttgcataag gtacgccgga tacggcgagg ccgataccgc
     5701 caagcactgc ctgtactaac gccgtcacca ccacacccag cgccaccgcg cggatagcct
     5761 gtgccgccag cagcacggcg gcatctccgc gaacgcctgc cagacgggtt gcaaaatggc
     5821 gaatgccttg tgccacctgt tcaccgcgcc agtacagcag ggcactgaag agaagcatca
     5881 gcgcacaatg caccataaag cgcccgatat gcgccgcctg cccaacgaac caggtggtgg
     5941 tggtgccaat ataagggcgg actttcgcca tgatcgccgt accccccata tccagcaagt
     6001 tgtgccagcc tgcatacagc ttcgcgccaa tcaccggaat ggtattaagc cacgccagat
     6061 cgggtaacgt catgtcgccg ctggaaatgg ctttaattag cgggccgctg ccgtcgacga
     6121 tactgttaac cagcaaagca atagggatga taaacaccat cactaataac agcgtcatca
     6181 ccagaacggc gagagagcgg cggccaaaca tgatcttttg caaacgtaac aataccggcc
     6241 aggtggcgat aaccaccgta ccggcccatg caaagccgag aataaagggt tgaacaatcc
     6301 acagacatgc cacaatcatg atggctaaaa acagcaccga aagcagaatt tgtgcgacat
     6361 ccctgggctg acgaacattt accataacta cttttcacct tttttgtgcg tcaaatcgtc
     6421 gacgcgacgt gaacacgcga aactcacctg cataatgatg agcaatttca gcggttttta
     6481 acaggccgat tctgccctta attctgatgg gcgtgttaga aaaaaatgtg gtacaacaac
     6541 tcaggcaaca cgcaaacgat ttactcgtcg tatttcaagt tgcatgtgct gcggctacgt
     6601 tcgttcaccc cagtcactta ctattgtaag ctcctggagt tcactcgctt gccgccttcc
     6661 tgcaactcga aatccataga gtaaataaac atcacctttc agtgcgctat tgcagccgcc
     6721 tgaaagaata gtgtatcgac ctacatcaca gaccgcagga aagggtcaat ataatgattc
     6781 cacagatttc cctggcaccc ggcgtcgttc aactggtgct taattttttg caagagctgg
     6841 agcaacaagg ttttaccggc gatgcggcga caagttatgc cgatcgtctg acaatgtcga
     6901 ccgacaacag tatttaccaa cttctccccg atgcggtggt atttccgcgt tcaaccgcag
     6961 atgtggcgct gatcgcccgt cttgccgcgc aggaacgcta ttcatcgctg atctttaccc
     7021 cccgcggcgg cggcaccggc actaacggtc aggcgctcaa ccaggggatt attgttgata
     7081 tgtcccgcca tatgaaccgc atcatcgaaa ttaaccctga agagggctgg gtgcgcgttg
     7141 aggccggggt gataaaagac caactcaatc agtacctgaa accgttcggc tacttttttg
     7201 cgccggaact ttcgaccagc aaccgggcaa cgctcggtgg gatgatcaat accgatgcat
     7261 ccggtcaggg atcgctggtc tatggcaaaa cgtcagatca cgtacttggc gtacgcgcgg
     7321 tgttgttggg gggcgatatt ctcgatacgc aacctttacc cgtcgagctg gcggaaacgc
     7381 tgggtaaatc caataccaca atcgggcgaa tttataacac ggtttatcaa cgttgccgtc
     7441 agcaacgcca gttaatcatc gacaacttcc ccaaacttaa ccgctttctt accggttacg
     7501 atctgcgtca tgtctttaac gatgagatga ccgagttcga cctgacgcgc attctgacgg
     7561 gttcagaagg gacgctggcc tttattaccg aagcgcggct ggatattacg cgcttgccta
     7621 aagtgcgccg tctggtgaat gtcaaatatg actcttttga ctctgcgctg cgtaacgcgc
     7681 cgtttatggt tgaggcgcgg gcgctttcgg tagagacggt ggactcaaaa gtgctgaatc
     7741 tggcgcggga agatattgtc tggcattccg tcagcgagtt gattaccgat atgcctgaca
     7801 aagagatgct cggactgaac attgtggaat ttgctggtga tgatgaggcg ctgattgacg
     7861 agcgagttaa tgcactctgt gtgcggcttg atgagctgat tgccagccaa caggcaggtg
     7921 tgattggctg gcaggtgtgc cgcgagctgg cgggggttga acgtatctat gcgatgcgca
     7981 aaaaagccgt tggtctgctt ggcaatgcca aaggtgccgc taagccaatt ccgtttgctg
     8041 aggatacctg cgtaccgccg gaacatctgg ccgattatat tgctgaattt cgcgcgctgc
     8101 tcgacagcca cggcttaagc tacggtatgt tcggtcacgt cgacgcaggt gtcttgcacg
     8161 tccgtccggc actggatatg tgcgatccgc aacaagagat tttgatgaag caaatctctg
     8221 atgacgtggt ggcgctgact gcgaaatacg gtggtttgtt gtggggcgag cacggcaaag
     8281 gttttcgcgc tgaatacagc ccggcgtttt tcggtgagga actttttgca gaactgcgca
     8341 aagtgaaagc ggcatttgac ccgcataacc gactcaaccc agggaagatt tgcccgccag
     8401 aaggtctcga tgcgccgatg atgaaagtgg acgcggtgaa gcgcggtaca ttcgatcggc
     8461 agatccccat tgcggtacgc cagcagtggc gcggtgcgat ggagtgtaac ggcaacggtt
     8521 tatgcttcaa ctttgatgcc cgtagtccga tgtgtccgtc gatgaagatc acccagaacc
     8581 ggattcattc accgaaaggg cgcgcaacgc tggtgcgtga atggctgcgc ttgttggcgg
     8641 atcgcggcgt tgacccactc aaactggaac aagaactgcc tgaatccggc gtcagtttgc
     8701 ggacgttaat tgcccgcacg cgcaatagct ggcatgcgaa taaaggcgaa tatgacttct
     8761 cacacgaagt caaagaggcg atgtcgggct gtctggcctg taaagcttgt tcgacccagt
     8821 gccccatcaa aattgatgtg ccggagtttc gctctcgttt tctgcagctc tatcacaccc
     8881 gttatttacg cccgcttcgc gaccacctcg ttgctacggt cgagagctac gcaccgctga
     8941 tggcacgcgc gccgaaaacc tttaacttct tcattaacca gccgctggtg cgcaaactct
     9001 cggaaaaaca tatcggcatg gttgatttgc cactgctgtc ggtcccctcg ctacaacaac
     9061 aaatggtggg gcatcgctcg gcaaacatga cgctggaaca gcttgaagct ctcaatgcag
     9121 agcagaaagc gcgcacggtg ttggtggtgc aggacccctt taccagctat tacgatgcgc
     9181 aagtggtggc ggattttgtc cgtctggttg aaaaattagg tttccagcct gtgttactgc
     9241 cattttcgcc aaatggcaaa gcccagcata tcaaaggctt tcttaatcgt tttgcgaaga
     9301 cggcgaaaaa gacggcggat ttcctcaacc gtatggctaa gcttgggatg ccaatggtgg
     9361 gcgtcgatcc ggcgctggta ctttgttatc gcgatgaata taaactggcg ctgggcgagg
     9421 aacgtggcga gtttaacgtc ttacttgcga atgaatggct ggcaagcgca cttgagtcac
     9481 atccagtggc tacagtcagc ggtgaatcat ggtatttctt tggtcactgt accgaggtta
     9541 ccgctttgcc gggcgcgcca gcacaatggg ccgcgatatt tgcccgtttt ggcgcgaaac
     9601 tggaaaatgt cagcgtgggt tgctgcggca tggcagggac ttacggacat gaagcgaaaa
     9661 accatgaaaa ttcgctcggg atctatgagt tatcctggca tcaggctatg cagcgactgc
     9721 cgcgtaaccg ctgtctggcg accggatatt cctgccgtag ccaggtaaaa cgggttgaag
     9781 gcacgggggt acgccatcct gtgcaggctt tactggagat tattaaatga tatggaaacg
     9841 gaaaatcacc ctggaagcac tgaatgctat gggtgaagga aacatggtgg ggttcctgga
     9901 tattcgcttt gaacatattg gtgatgacac ccttgaagcg acaatgccag tagactcgcg
     9961 gacaaagcag cctttcgggt tgctgcatgg aggagcatcc gtggtactgg ccgaaagtat
    10021 cggttccgtt gccggttatt tatgtaccga aggtgagcaa aaagtggttg gtctggaaat
    10081 caatgctaat cacgtccgct cggcacgaga agggcgggtg cgcggcatat gtaaaccgtt
    10141 gcatctcggt tcgcgtcacc aggtctggca gattgaaatc ttcgatgaga aagggcgttt
    10201 gtgctgttcg tcacgattga cgaccgccat tttgtgacgt tgtcaccaga aaagtgtgac
    10261 gcatattgct tttggtcatt atctgcacag taaacggtgg tatactgtgc aggtcttgtc
    10321 taaaagcgag gatgctatgt ctactcaact tgatcccacc cagctggcca ttgaattttt
    10381 acgccgtgac cagagcaatc tttcacctgc ccaatatctc aaacgtctga aacaactaga
    10441 acttgaattt gctgacctgc ttactctctc ttctgctgaa ttaaaagaag aaatctactt
    10501 tgcctggcgt ctgggcgtgc attgatcctg acttaatgcc gcaagttcgc caaatcgacc
    10561 tggattaacc tccgtccagg ttcgatgacc ttcttaatac tgcttatcta ccgcctgata
    10621 ttgttcaaaa ataatactaa tcgcagtttg tgttaaaacg gcgggttagc tttatgagat
    10681 tattcttatc gccccttcaa gagctaagcc actgagagtg ccggagataa gcgccggatg
    10741 gggtagaaac ccttaagcct gtgtcgcaca gacttaaggg ttttcttatt tctgaacgct
    10801 gcactttttt acctatctct taaatagtca ttttcaatac aactttttct ttttcatcac
    10861 agccttaatg ctccttagat aataaccatt atctaacaat gagataccta attcttagcg
    10921 tgcctgttaa cccacacatc aggggctatg cttattaaaa gcaggcaaaa agaatgtttt
    10981 acggtaaagc ccctgcgttt gctgggttga actgataatc attatcacta acatgctgtt
    11041 atacgctgaa agcgatgaag tgaggtaaat agatggacat gcattcagga acctttaacc
    11101 cacaggattt cgcctggcaa ggcttaacgc tgacacccgc agcggcgata cacatccgcg
    11161 agctggtggc aaagcagcca ggtatggtcg gcgtgcgctt aggtgtgaag caaacgggct
    11221 gcgcgggctt tggctatgtg ctcgacagtg ttagcgagcc ggacaaagac gatctgctgt
    11281 ttgaacacga cggcgcgaag ctgtttgtcc cgctgcaatc gatgccgttt attgatggca
    11341 cggaagtcga tttcgttcgt gaaggactta atcagatatt caaatttcac aaccctaaag
    11401 cccagaatga atgtggctgt ggcgaaagct ttagggtata ggcggtacta tgtctcgtaa
    11461 tactgaagca actgacgatg tcaaaacctg gaccggcggc ccgctgaact ataaagaagg
    11521 attcttcacc cagttagcca ccgatgagct ggcaaagggg ataaacgaag aggtggtgcg
    11581 cgcaatttcg gcgaagcgta atgagccgga gtggatgctg gagtttcgtc taaacgccta
    11641 tcgcgcatgg ctggagatgg aagaaccgca ctggttgaaa gcgcactacg acaagctgaa
    11701 ttatcaggat tacagctact actcagcacc atcgtgcggt aattgtgacg acacttgcgc
    11761 gtctgaacct ggcgcggtgc agcaaactgg cgcgaacgcc tttttaagta aagaggtgga
    11821 ggcggcgttt gagcagttgg gcgttcccgt gcgggaaggc aaagaggtgg cggtggatgc
    11881 cattttcgac tctgtttcgg ttgccaccac ttatcgcgaa aaactggcgg agcagggaat
    11941 tattttctgt tcctttggtg aggcgatcca cgatcacccg gaactggtgc gtaaatatct
    12001 cggcaccgtg gtgccgggga atgacaactt ctttgcagca cttaatgcgg cggtagcctc
    12061 tgacggtacg tttatttatg tgcctaaagg cgtgcgctgc ccgatggaac tttccaccta
    12121 ttttcgcatt aacgcggaaa aaaccgggca gtttgagcgc acaattctgg tggccgacga
    12181 agacagctac gtcagctaca ttgaaggctg ttccgctccg gtgcgtgaca gctatcagtt
    12241 gcacgcggca gtggtggaag tcatcatcca taaaaacgcc gaggtgaaat attccacggt
    12301 acaaaactgg ttccctggcg ataacaacac cggcggtatt ctcaacttcg tcaccaagcg
    12361 tgctttgtgc gaaggcgaaa acagcaaaat gtcatggacg caatcagaaa ccgggtcagc
    12421 gattacgtgg aaatatccca gctgcatttt gcgcggcgat aactccattg gtgagtttta
    12481 ctcagtggcg ctgaccagcg gtcatcagca agcggatacc ggcaccaaga tgatccacat
    12541 cggtaaaaac accaaatcga ccattatctc gaaagggatc tctgccggac atagtcagaa
    12601 cagttatcgc ggcttagtga aaatcatgcc gacggcaacc aatgcgcgca atttcactca
    12661 gtgcgactca atgctgattg gcgctaattg tggggcgcat accttcccgt atgtcgaatg
    12721 tcgcaataac agcgcacaac tggagcacga ggcaacgaca tcacgtattg gtgaagatca
    12781 actgttttac tgcctgcaac gcgggatcag cgaagaagac gccatctcga tgattgttaa
    12841 cggtttctgc aaagacgtgt tctcagaact gccgctggaa tttgccgttg aagcacaaaa
    12901 actcctcgcc atcagtcttg aacacagcgt cggataagga ataaacatgt taagtattaa
    12961 agatttacac gtcagcgtgg aagataaagc tatcctgcgt ggattaagcc tcgacgttcg
    13021 tcccggcgaa gttcacgcca ttatggggcc aaacggttcg ggcaaaagta ccttatcggc
    13081 aacgcttgcc gggcgcgaag attatgaagt gacgggcggc acggttgagt tcaaaggcaa
    13141 agatttgctt gcactgtcgc cggaagatcg tgcaggcgaa ggcatcttta tggccttcca
    13201 gtatccggtg gagattccag gcgtcagtaa ccagtttttc ctgcaaacgg cacttaatgc
    13261 agtgcgcagc tatcgcggcc aggaaacgct cgaccgcttt gattttcagg atttgatgga
    13321 agagaaaatc gctctcctga agatgccgga agatttatta acccgttcgg taaacgttgg
    13381 tttttccggc ggcgagaaaa agcgcaacga tattttgcaa atggcggtgc tggaaccgga
    13441 gttatgcatt cttgatgagt cggactccgg gctggatatt gacgcattaa aagtggtcgc
    13501 cgatggcgtg aactcgctgc gtgatggcaa gcgctcattc atcattgtta cgcactacca
    13561 acgcattctc gactacatca agcctgatta cgttcatgtg ctatatcagg gacgaattgt
    13621 gaaatccggc gatttcacgt tggtcaaaca actggaggag cagggttatg gctggcttac
    13681 cgaacagcag taatgcgctg caacagtggc atcacttgtt tgaagctgaa gggacaaaac
    13741 gctcaccgca agcacagcag catttacaac aattgctgcg taccggactg ccgacacgta
    13801 aacatgaaaa ctggaaatat acgccgctgg aagggctgac caatagtcag tttgtcagca
    13861 ttgcgggaga tatatcccca cagcagcgtg atgccttagc gttaacgtta gacgccgtgc
    13921 ggctggtgtt tgtcgatgga cgttacgtgc ccgcgctgag cgatgcaatt gaaggcagcg
    13981 gatatgaagt gagcattaac gacgaccgtc aggggttacc cgacgctatt caggcggaag
    14041 tgtttctgca tttgacggaa agcctggcac aaagcgtgac gcatatcgcc gtgaagcgcg
    14101 gtcaacggcc ggcaaagcca ttgctgttaa tgcatatcac ccagggcgtg gcaggtgaag
    14161 aggtgaacac tgcccattac cggcatcatc tggatctggc ggaaggtgcc gaagcaacgg
    14221 tgatcgaaca ttttgtcagc cttaatgatg ctcgtcattt taccggggca cggttcacta
    14281 tcaatgtcgc agcgaacgcc cacttgcagc atatcaagct ggcgtttgaa aacccggtca
    14341 gtcaccactt tgcccataac gatttgttgc tggctgacga tgccaccgca tttagccata
    14401 gtttcctgct gggtggcgca gtgttacgac acaacaccag tacgcaactc aatggcgaaa
    14461 acagcacgct gcggatcaat agcctggcga tgccggtgaa aaacgaggtg tgtgataccc
    14521 gtacctggct ggaacacaat aaaggttttt gtaacagccg acagttgcac aaaactatcg
    14581 tcagcgacaa aggccgcgcg gtatttaacg gtttgatcaa cgtcgcgcag cacgccatca
    14641 aaacggatgg tcagatgacc aacaataatc tgctgatggg caaactggcg gaagtggata
    14701 caaaaccgca gctggaaatc tatgcagatg atgtgaaatg cagccacggc gcgacggtgg
    14761 ggcgtattga tgatgaacag atgttctatc tgcgctcgcg cgggatcaat cagcaggatg
    14821 cccagcagat gatcatttac gcctttgctg ccgaactgac ggaagcactg cgtgatgagg
    14881 ggcttaaaca gcaggtgctg gcccgaatcg gtcaacggct gccaggaggt gcaagatgac
    14941 tttttccgtc gacaaagtgc gggccgactt tccggtgctt tcgcgtgagg taaacggttt
    15001 gccgctggct tatctcgaca gcgccgccag tgcacagaaa ccgagccagg tgattgacgc
    15061 cgaggccgag ttttatcgtc atggctacgc ggcggtgcat cgcggtattc ataccttaag
    15121 cgcccaggcg accgagaaaa tggagaacgt gcgcaagcgg gcatcgctgt ttattaatgc
    15181 ccgttcggcg gaagagctgg tgtttgtccg cggcacgacg gaagggatca atctggtcgc
    15241 caatagctgg ggcaacagca atgtgcgggc gggcgataac atcatcatca gtcagatgga
    15301 gcaccacgct aacattgtcc cttggcagat gctttgcgca cgcgttggcg cagagctgcg
    15361 tgtgatccct ctcaaccccg acggtacgct gcaactggag acgctgccta cgctgtttga
    15421 tgagaaaact cgcctgctgg caattactca tgtctccaac gtgcttggca cagaaaatcc
    15481 actggcggaa atgatcacgc ttgcgcacca gcatggcgca aaagtgctgg tggatggcgc
    15541 tcaggcggtg atgcatcatc cggtggatgt tcaggcgctg gattgcgact tttacgtgtt
    15601 ctccgggcat aaactgtatg gccccaccgg aattggcatt ctttatgtga aagaagcctt
    15661 gttgcaggag atgccgccgt gggaaggggg cggttctatg atcgccaccg tcagcctgag
    15721 tgaaggcact acctggacca aagcaccatg gcggtttgaa gccggtacac ccaataccgg
    15781 gggcatcatt ggtcttggcg cggcgctgga gtatgtttcg gcgctggggc ttaatagcat
    15841 agccgagtat gaacagaatc tgatgcatta tgcgctatca cagctggaat ctgtaccgga
    15901 tctcactctc tatggccaac aaaacaggct tggcgttatt gcttttaatc tcggtaaaca
    15961 ccacgcctat gatgttggca gttttctcga taattacggc attgctgtgc gtaccggaca
    16021 tcactgcgct atgccattaa tggcctatta caacgtccct gcgatgtgtc gggcgtcgct
    16081 ggccatgtat aacacccatg aagaagtgga gcgtctggtg accggcctgc aacgtattca
    16141 ccgtctgctg ggataacagg gaggcactat ggctttattg ccggataaag aaaaattgct
    16201 gcgtaatttt ttacgctgcg ccaactggga agagaaatat ctctacatta ttgagctggg
    16261 ccagcgtctg ccagaattac gcgccgaaga cggaagccca caaaatagca ttcagggctg
    16321 ccagagtcag gtgtggattg tcatgcgcca gaatgcgcag ggaattattg aattgcaggg
    16381 cgacagcgat gcggcgattg tgaaagggct tattgcggtc gtctttattc tctacgatca
    16441 gatgacgccg caggatattg tcaatttcga tgtgcgtccg tggtttgaaa aaatggcgct
    16501 cacccaacat ctcaccccat ctcgttcaca aggtctggaa gcgatgattc gcgcaattcg
    16561 cgccaaagcc gctgcactta gctaaactaa agggacagct ttcatccggt tggctcacat
    16621 tgccggatgg tcaatgttta cactgaccgg cacaggagtg ttttggtttc aggtgaacat
    16681 aagcaccatc cgacgtttca gcgtgagtct ccggcaaata caggaggttt acaatgaaac
    16741 gcgcgtcttt gcttacactc acgcttatcg gcgcttttag cgccatccag gctgcctggg
    16801 cggttgatta tccgctacca ccaaccggaa gccgactggt tgggcaaaat caaacgtata
    16861 cggtgcaaga aggggataaa aaccttcagg ctattgcccg acgttttgat actgcagcaa
    16921 tgttgatcct tgaagccaat aacactatcg ccccggtgcc aaaacctggt acgacgataa
    16981 ctattccttc acaactgtta ttacctgatg caccgcgtca ggggattatc gttaaccttg
    17041 cagagctgcg cctttattat tatccgccgg gagaaaatat tgtgcaggtt tatccaatag
    17101 gtattggatt gcaggggctg gaaacgccgg tgatggaaac gcgtgttggg cagaaaatcc
    17161 ctaacccaac ctggacgcct acggcaggca ttcgtcagcg ttcgctggag cgtggcatta
    17221 aattaccgcc agtcgttcct gccggaccaa ataacccgct aggacgttac gcactgcgcc
    17281 tcgcgcatgg taatggcgaa tacctcattc atggcaccag tgcgccggac agcgttggtt
    17341 tgcgcgtcag ttcagggtgt attcgcatga atgcaccgga tattaaagcc ttgttctcca
    17401 gcgtgcggac gggaacgccg gtgaaagtga tcaacgaacc ggtgaaatat tccgtggaac
    17461 ctaacgggat gcgttatgtt gaagtacatc gaccactatc agcagaagaa cagcaaaacg
    17521 ttcagacaat gccatacaca ctgccagcag gctttacgca atttaaagac aataaggctg
    17581 tagatcagaa gttagtcgat aaagcgttgt atcgtcgggc agggtatccg gtttcggtga
    17641 gcagtggagc aactcccgca gccagcaatg cgccttcagt agagtcagcg cagaatggtg
    17701 aaccagagca agggaatatg ttacgcgcga cgcagtagtg gtaaatagca gacaaaaaaa
    17761 tggcgcacaa tgtgcgccat ttttcacttc acaggtacta ttacttgcgg tatttagtag
    17821 ccatgttgtc cagacgctgg ttagcacgag ctgcgtcatc tttagcagcc tgaacgtcgg
    17881 aacgcattgc gttcacgtcg ttgctcagct ggtcaacttt agcgttcaga gtctgaacgt
    17941 cagaagacag ctgatcgatt ttagcgttgc tggagcaacc tgccagcaga gtagaaccca
    18001 ggattaccgc gcccagtacc agtttagtag ctttcattat taataccctc tagattgagt
    18061 taatctccat gtagcgttac aagtattaca caaagttttt tatgttgaga atattttttt
    18121 gatgggaagg cacttatttt tgatcgttcg ctcaaagaag catcgaacgc tgggttttag
    18181 ataaaaaaat tgagagaaaa caggatgctt ccatcggatt catcttaaat aaagtgagat
    18241 tatatagtga aatccgattt gattttttat tgcttctggt tatcgattaa ataaaaaaag
    18301 cgcccatcag ggcgcttcga tatacaaatt aattcacaaa agcaatatta caggacgtga
    18361 acagatgcgg tgttagtagt gccgctcggt accagtgcac cagaaaccat aactacgacg
    18421 tcacctttgt gtgccagacc gctctgcaga gccagttctt tacccagacg gtagaaatca
    18481 tcagtagaag tgatctcttt aaccagctgc ggcacaacgc ctttgctcag taccaactga
    18541 tgagccgttt tttcgttggt ggtcagtgcc aggatggtgg catccgggaa gtatttacgt
    18601 actgcgcgag cagatttacc gccctgggta gcaaccacga tcagcggagc atccagtttt
    18661 tcagcagttt caacggcacc acggcatacc gcttcggtga tgcgcagttt acggttgtca
    18721 ttgttgaact cgagacggct gttcatcacg cggtcggtac gttcgcagat ggtcgccatg
    18781 atagaaaccg cttccagcgg gtatttacct tttgcggatt caccagacag catcactgcg
    18841 tcagtaccgt cgaggatggc gtttgcaacg tcaccggctt ctgcgcgagt cgggcgtggg
    18901 tttttgatca tggaatccag catctgggtc gcagtgataa cgactttacg tgcacggata
    18961 catttttcga tcatcatctt ctgggcgaag ataacttctt ctaccgggat ttctacaccc
    19021 aggtcgccac gcgcaaccat gatgccgtca gaggcttcga ggatttcgtc gaagttgttg
    19081 aggccttcct ggttttcgat tttggagatg atgtggatgt tttcgccgcc gtgcgctttc
    19141 aggtgctcac ggatttcgat aacgtcagaa cgcttacgaa taaaggaagc agcaacaaag
    19201 tctacgcctt tttcgcaacc aaagatcagg tcctgtttgt ctttttcagc cagtgctggc
    19261 agagcaatgg aaacgccagg caggttcaca cctttgtttt cgcccaggtc accgttgttc
    19321 agcactttac agataacttt gttaccttca atggcggtaa cttccatacc gatcagacca
    19381 tcgtcaacca gtacggtgtt gccaacagac aggtcagtag tgaaaccttc atacgttacc
    19441 gcaaccattt cgctgttgcc gataacagat ttatcagtgg tgaaagtaaa ggtctgacca
    19501 gctttcagag aaacatcgtt accgccttcc agtttcatgg tgcggatttc cggacctttg
    19561 gtatcaagca ggatagcggc ggttttacca gttttgctca tcacgttgcg cagattctga
    19621 atgcgctgac cgtgttctgc atagtcacca tgagagaagt tcagacgcat aacgttcatg
    19681 ccagcgtcca gcattttagc taacatctct tcagattcgg ttttcggtcc gatggtgcaa
    19741 acaattttgg tctttttcat gacagtctta gtctttaagt tgagaaggat gggagaaact
    19801 tgctttctgg gcgcactgcc ggaaagcgaa tccggtatta cgaaagtggt ggtgcctcgc
    19861 tctaaggata ggtgacatcg aaaaagcgtg cagaggaatg tgtgcttgtg tctcagccca
    19921 acgtgagtgt ctgtttttga gtcgttgagt tctacagtcc aaagtgctga aaccattcaa
    19981 gagtcaattg gcgcgcatta tacgctgaaa ttaatccaaa aggaaaacaa aaacagcgtt
    20041 tcaaaaaata tacaacttgt tagacaataa attgttatca aagatttaac gttccaggga
    20101 aaaggttacg caaaaacttc gctgtttgcg caacgtctgg tgccgaagtt gtcagtgtga
    20161 ggataagtga aagttcatcc ggtgtataaa tatatcatcg ctcaattgct acagttaaaa
    20221 taatttatcg cgttatttgt ctgattttgt ttgatttttg tttaattggt tagattga
//
LOCUS       DAJTRD010000074        19692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTRD010000074 DAJTRD010000000
VERSION     DAJTRD010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.074"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO315_002856"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO315_002856"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5891697.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO315_002857"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO315_002857"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5891698.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO315_002858"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO315_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5891699.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO315_002859"
     CDS             6038..6658
                     /locus_tag="OO315_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5891700.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO315_002860"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO315_002860"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5891701.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO315_002861"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO315_002861"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5891702.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO315_002862"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO315_002862"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5891703.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO315_002863"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO315_002863"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5891704.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO315_002864"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO315_002864"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="OO315_002865"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="OO315_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="OO315_002866"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="OO315_002866"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5891705.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14226
                     /gene="ttdT"
                     /locus_tag="OO315_002867"
     CDS             12769..14226
                     /gene="ttdT"
                     /locus_tag="OO315_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5891706.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVTLIVVFYLLRYFF
                     ASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYGSG
                     YLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14269..15282)
                     /gene="tsaD"
                     /locus_tag="OO315_002868"
     CDS             complement(14269..15282)
                     /gene="tsaD"
                     /locus_tag="OO315_002868"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5891707.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15520..15735
                     /gene="rpsU"
                     /locus_tag="OO315_002869"
     CDS             15520..15735
                     /gene="rpsU"
                     /locus_tag="OO315_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5891708.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15846..17591
                     /gene="dnaG"
                     /locus_tag="OO315_002870"
     CDS             15846..17591
                     /gene="dnaG"
                     /locus_tag="OO315_002870"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5891709.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17786..19627
                     /gene="rpoD"
                     /locus_tag="OO315_002871"
     CDS             17786..19627
                     /gene="rpoD"
                     /locus_tag="OO315_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5891710.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         4681 a         5506 c         5029 g         4476 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtgtcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgacgtt gattgtggtg
    13921 ttttatctac tgcgctactt tttcgccagc gccacggcgt atacctgcgc cctcgcaccg
    13981 atgatgatcg ccgccgcgct ggcgatgccg gaaatcccgc tgccggtgtt ctgcctgatg
    14041 gttggcgcgg caattggtct ggggagcatt cttacaccat acgccaccgg acccagtccg
    14101 atttactacg gtagtggtta tctgccaacg gcggattact ggcgactggg ggcgattttt
    14161 gggctgatat tcctcgtgtt gctggtgatt accggcttac tgtggatgcc cgtggtgttg
    14221 ctttaagtga ggacggtgcc ggaaattgct ctccggcacc caatagtttt acgcagccgg
    14281 taactccgcc agcggccagc gcggacgcac gctaacgccg agatccgccg tcgcgcctgc
    14341 tttaaaccgc accattccgg cataggcgat catcgcgccg ttatcagtac aaaattccgg
    14401 acgcgcgtag aacacttcgc cgcggcgttt tttcatcatt tcagccagct tcgcccgtaa
    14461 cgtgcggtta gcactcacgc cgcccgccat gaccagtcgc ttaaagcccg tctgatccaa
    14521 cgctcgctta cacttaatca tcaacgtatc gaccaccgca tcttcaaagg cgcgggcgat
    14581 atcagcacgc gtctggtcgt cggtgccgtt gtcacgaatc gtgttcgccg caaaggtttt
    14641 cagaccagaa aagctgaaat ccagccccgg acggtcggtc atcggacgcg ggaaaacaaa
    14701 gcgcccggca gtaccctgag ccgccatttt cgacagtaac ggtccgccag gataatccag
    14761 ccccagcagc ttcgcggttt tatcaaacgc ttcaccggcg gcatcatcga tagactcgcc
    14821 gagcagctcg tactgaccaa tgccagtcac gctgattaac tgcgtatgac cgccggacac
    14881 cagcagcgcg acaaacggaa attccggcgg gttatcttcc agcatcggcg ctaacagatg
    14941 cccttccata tggtgtaccg ggattgccgg aacgttccag gcaaacgcca gagaacgccc
    15001 cacggttgcg ccaaccagca gtgcgccgac taatccaggg cctgcggtat aggccacagc
    15061 atcaatgtct tttgccgtta aaccagactc ctttagcgcc gcctggatca acggtacggt
    15121 tttacgcaca tgatcgcggg aggccagttc aggcacgacg ccgccgtagt cagcgtgcaa
    15181 tttcacctga ctatacaatt ggttggctaa caaacctttt tcatcgtcgt aaatggcgat
    15241 gccggtttca tcgcaggaag tttcaatacc cagtacacgc atgacttgtt ttacctcgct
    15301 ttattaccgc gcagtgtagg accaatgcgg gttgatgtaa aactttgttc gcccctggag
    15361 aaagcctcgt gtatactcct cacccttata aaagtccctt tcaaaaaagg ccgcggtgct
    15421 ttacaaagca gcagcaattg cagtaaaatt ccgcaccatt ttgaaataag ctggcgttga
    15481 tgccagcggc aaaccgaatt aatcaaaggt gagaggcaca tgccggtaat taaagtacgt
    15541 gaaaacgagc cgttcgacgt agctctgcgt cgcttcaagc gttcctgcga aaaagcaggt
    15601 gttctggcgg aagttcgtcg tcgtgagttc tatgaaaaac cgactaccga acgtaagcgc
    15661 gctaaagctt ctgcagtgaa acgtcacgcg aagaaactgg ctcgcgaaaa cgcacgccgc
    15721 actcgtctgt actaattccc cgagagcgtt gctctccgat cagaccgagt tgtagttgta
    15781 aggccgtgct tccgaaagga atgcgcggct tattttcgtt tatgaattgc taaaaatcgg
    15841 ggcctatggc tggacgaatc ccacgcgtat tcattaatga tctgctggca cgcactgaca
    15901 tcgtcgatct gatcgatgcc cgtgtgaagc tgaaaaagca gggcaagaat ttccacgcgt
    15961 gttgtccatt ccacaacgag aaaaccccgt cattcaccgt taacggtgag aaacagtttt
    16021 accactgctt tggatgtggc gcgcacggca acgcgatcga cttcctgatg aactacgaca
    16081 agctcgagtt cgtcgaaacg gtcgaagagc tggcagcaat gcacaatctt gaagtgccat
    16141 ttgaagcagg cagcggcccc agccagatcg agcgccatca gaggcaaacg ctttatcagt
    16201 tgatggacgg tctgaatacg ttttaccaac aatctttaca acaacctgtt gccacgtctg
    16261 cgcgccagta tctggaaaaa cgcggattaa gccacgaggt tatcgctcgc tttgcgattg
    16321 gttttgcgcc ccccggctgg gacaacgtcc tgaagcggtt tggcggcaat ccagaaaatc
    16381 gccagtcatt gattgatgcg gggatgttgg tcactaacga tcagggacgc agttacgatc
    16441 gtttccgcga acgggtgatg ttccccattc gcgataaacg cggtcgggtg attggttttg
    16501 gcgggcgcgt gctgggcaac gataccccca aatacctgaa ctcgccggaa acagacattt
    16561 tccataaagg ccgccagctt tacggtcttt atgaagcgca gcaggataac gctgaaccca
    16621 atcgtctgct tgtggtcgaa ggctatatgg acgtggtggc gctggcgcaa tacggcatta
    16681 attacgccgt tgcgtcgtta ggtacgtcaa ccaccgccga tcacatacaa ctgttgttcc
    16741 gcgcgaccaa caatgtcatt tgctgttatg acggcgaccg tgcaggccgc gatgccgcat
    16801 ggcgagcgct ggaaacggcg ctgccttaca tgacagacgg ccgtcagcta cgctttatgt
    16861 ttttgccgga tggcgaagac cctgacacgc tggtacgaaa agaaggtaaa gaagcgtttg
    16921 aagcgcggat ggagcaggcg atgccactct ccgcatttct gtttaacagt ctgatgccgc
    16981 aagttgatct gagtacccct gacgggcgcg cacgtttgag tacgctggca ctgccattga
    17041 tatcgcaagt gccgggcgaa acgctgcgaa tatatcttcg tcaggaatta ggcaacaaat
    17101 taggcatact tgatgacagc cagcttgaac gattaatgcc aaaagcggca gagagcggcg
    17161 tttctcgccc tgttccgcag ctaaaacgca cgaccatgcg tatacttata gggttgctgg
    17221 tgcaaaatcc agaattagcg acgttggtcc cgccgcttga gaatctggat gaaaataagc
    17281 tccctggact tggcttattc agagaactgg tcaacacttg tctctcccag ccaggtctga
    17341 ccaccgggca acttttagag cactatcgtg gtacaaataa tgctgccacc cttgaaaaac
    17401 tgtcgatgtg ggacgatata gcagataaga atattgctga gcaaaccttc accgactcac
    17461 tcaaccatat gtttgattcg ctgcttgaac tgcgccagga agagttaatc gctcgtgagc
    17521 gcacgcatgg tttaagcaac gaagaacgcc tggagctctg gacattaaac caggagctgg
    17581 cgaaaaagtg atttaacggc ttaagtgccg aagagcgatc gggaagcccc cgacagccgc
    17641 actgagaggc agcggcaaat atataagtac gccctcgtaa ttatcgttgg cggtaaacaa
    17701 ccgttggatt tcagcgttaa cggctgaagg acatcgggtc aatcgcccaa caccaacctc
    17761 atgaaataag tgtggatacc gtcttatgga gcaaaacccg cagtcacagc tgaaacttct
    17821 tgtcacccgt ggtaaggagc aaggctatct gacctatgcc gaggtcaatg accatctgcc
    17881 ggaagatatc gtcgattcag atcagatcga agacatcatc caaatgatca acgacatggg
    17941 cattcaggtg atggaagaag caccggatgc cgatgatctg atgctggctg aaaacaccgc
    18001 ggacgaagat gctgccgaag ccgccgcgca ggtgctttcc agcgtggaat ctgaaatcgg
    18061 gcgcacgact gacccggtac gcatgtacat gcgtgaaatg ggcaccgttg aactgttgac
    18121 ccgcgaaggc gaaattgaca tcgctaagcg tattgaagac gggatcaacc aggttcaatg
    18181 ctccgttgct gaatatccgg aagcgatcac ctatctgctg gaacagtacg atcgtgttga
    18241 agcagaagaa gcgcgtctgt ccgatctgat caccggcttt gttgacccga acgcagaaga
    18301 agatctggca cctaccgcca ctcacgtcgg ttctgagctt tcccaggaag atctggacga
    18361 tgacgaagat gaagacgaag aagatggcga tgacgacagc gccgatgatg acaacagcat
    18421 cgacccggaa ctggctcgcg aaaaatttgc ggaactacgc gctcagtacg ttgtaacgcg
    18481 tgacaccatc aaagcgaaag gtcgcagtca cgctgccgct caggaagaga tcctgaaact
    18541 gtctgaagta ttcaaacagt tccgcctggt gccgaagcag tttgactacc tggtcaacag
    18601 catgcgcgtc atgatggacc gcgttcgtac gcaagaacgt ctgatcatga agctctgcgt
    18661 tgagcagtgc aaaatgccga agaaaaactt cattaccctg tttaccggca acgaaaccag
    18721 cgatacctgg ttcaacgcgg caattgcgat gaacaagccg tggtcggaaa aactgcacga
    18781 tgtctctgaa gaagtgcacc gcgccctgca aaaactgcag cagattgaag aagaaaccgg
    18841 cctgaccatc gagcaggtta aagatatcaa ccgtcgtatg tccatcggtg aagcgaaagc
    18901 ccgccgtgcg aagaaagaga tggttgaagc gaacttacgt ctggttattt ctatcgccaa
    18961 gaaatacacc aaccgtggct tgcagttcct tgacctgatt caggaaggta acatcggcct
    19021 gatgaaagcg gttgataaat tcgaataccg ccgtggttac aagttctcca cctacgcaac
    19081 ctggtggatc cgtcaggcga tcacccgttc tatcgcggat caggcgcgca ccatccgtat
    19141 tccggtgcat atgattgaga ctatcaacaa actcaaccgt atttctcgcc agatgctgca
    19201 agagatgggc cgtgaaccga cgccggaaga actggctgaa cgtatgctga tgccggaaga
    19261 caagatccgc aaagtgctga agatcgccaa agagccaatc tccatggaaa cgccgatcgg
    19321 tgatgatgaa gattcgcatc tgggggattt catcgaggat accaccctcg agctgccgct
    19381 ggattctgcg accaccgaaa gcctgcgtgc ggcaacgcac gacgtgctgg ctggcctgac
    19441 cgcgcgtgaa gcaaaagttc tgcgtatgcg tttcggtatc gatatgaaca ccgaccacac
    19501 gctggaagaa gtgggtaaac agttcgacgt tacccgcgaa cgtatccgtc agatcgaagc
    19561 gaaggcgctg cgcaaactgc gtcacccgag ccgttctgaa gtgttgcgta gcttcctgga
    19621 cgattaatcg gtaggccgga tcaggcgtta cgccgcaccc ggcactagtc tctctgcaca
    19681 aacgccacct tt
//
LOCUS       DAJTRD010000075        19379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTRD010000075 DAJTRD010000000
VERSION     DAJTRD010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.075"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OO315_002872"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OO315_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5891711.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OO315_002873"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OO315_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5891712.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO315_002874"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO315_002874"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5891713.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTLSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OO315_002875"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OO315_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5891714.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OO315_002876"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OO315_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5891715.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OO315_002877"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OO315_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5891716.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OO315_002878"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OO315_002878"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5891717.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OO315_002879"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OO315_002879"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5891718.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OO315_002880"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OO315_002880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5891719.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OO315_002881"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OO315_002881"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5891720.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OO315_002882"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OO315_002882"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5891721.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OO315_002883"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OO315_002883"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5891722.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OO315_002884"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OO315_002884"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5891723.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OO315_002885"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OO315_002885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5891724.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OO315_002886"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OO315_002886"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5891725.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OO315_002887"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OO315_002887"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5891726.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OO315_002888"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OO315_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5891727.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OO315_002889"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OO315_002889"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5891728.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
BASE COUNT         4771 a         4857 c         5119 g         4632 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cagggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatatgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgccgaccg
//
LOCUS       DAJTRD010000076        18803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTRD010000076 DAJTRD010000000
VERSION     DAJTRD010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.076"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(139..3654)
                     /locus_tag="OO315_002890"
     CDS             complement(139..3654)
                     /locus_tag="OO315_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5891729.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO315_002891"
     CDS             complement(4107..5369)
                     /locus_tag="OO315_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5891730.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO315_002892"
     tRNA            complement(5567..5642)
                     /locus_tag="OO315_002892"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO315_002893"
     CDS             complement(5748..6455)
                     /locus_tag="OO315_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5891731.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO315_002894"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO315_002894"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5891732.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO315_002895"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO315_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5891733.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO315_002896"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO315_002896"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5891734.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO315_002897"
     CDS             complement(11639..11914)
                     /locus_tag="OO315_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5891735.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO315_002898"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO315_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5891736.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO315_002899"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO315_002899"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5891737.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO315_002900"
     CDS             13874..14200
                     /locus_tag="OO315_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5891738.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO315_002901"
     CDS             14384..15103
                     /locus_tag="OO315_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5891739.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO315_002902"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO315_002902"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5891740.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO315_002903"
     CDS             16442..17404
                     /locus_tag="OO315_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5891741.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18803)
                     /locus_tag="OO315_002904"
     CDS             complement(17505..>18803)
                     /locus_tag="OO315_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5891742.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4899 a         4584 c         4644 g         4676 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa ggccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cgatataact atc
//
LOCUS       DAJTRD010000077        18451 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTRD010000077 DAJTRD010000000
VERSION     DAJTRD010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18451
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.077"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..1238
                     /locus_tag="OO315_002905"
                     /pseudo
     CDS             1..1238
                     /locus_tag="OO315_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
     gene            1388..1819
                     /gene="ndk"
                     /locus_tag="OO315_002906"
     CDS             1388..1819
                     /gene="ndk"
                     /locus_tag="OO315_002906"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5891743.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            1969..3123
                     /gene="trmG/rlmN"
                     /locus_tag="OO315_002907"
     CDS             1969..3123
                     /gene="trmG/rlmN"
                     /locus_tag="OO315_002907"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5891744.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            3408..4421
                     /gene="rodZ"
                     /locus_tag="OO315_002908"
     CDS             3408..4421
                     /gene="rodZ"
                     /locus_tag="OO315_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5891745.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            4448..5575
                     /gene="ispG"
                     /locus_tag="OO315_002909"
                     /gene_synonym="gcpE"
     CDS             4448..5575
                     /gene="ispG"
                     /locus_tag="OO315_002909"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5891746.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            5678..6952
                     /gene="hisS"
                     /locus_tag="OO315_002910"
     CDS             5678..6952
                     /gene="hisS"
                     /locus_tag="OO315_002910"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5891747.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            6970..7590
                     /gene="yfgM"
                     /locus_tag="OO315_002911"
     CDS             6970..7590
                     /gene="yfgM"
                     /locus_tag="OO315_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5891748.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            7601..8779
                     /gene="bamB"
                     /locus_tag="OO315_002912"
     CDS             7601..8779
                     /gene="bamB"
                     /locus_tag="OO315_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5891749.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            8897..10369
                     /gene="der"
                     /locus_tag="OO315_002913"
     CDS             8897..10369
                     /gene="der"
                     /locus_tag="OO315_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5891750.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            10439..10654
                     /locus_tag="OO315_002914"
     CDS             10439..10654
                     /locus_tag="OO315_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5891751.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(10651..12021)
                     /gene="xseA"
                     /locus_tag="OO315_002915"
     CDS             complement(10651..12021)
                     /gene="xseA"
                     /locus_tag="OO315_002915"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5891752.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12183..13649
                     /gene="guaB"
                     /locus_tag="OO315_002916"
     CDS             12183..13649
                     /gene="guaB"
                     /locus_tag="OO315_002916"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5891753.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            13718..15295
                     /gene="guaA"
                     /locus_tag="OO315_002917"
     CDS             13718..15295
                     /gene="guaA"
                     /locus_tag="OO315_002917"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5891754.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(15390..15929)
                     /locus_tag="OO315_002918"
     CDS             complement(15390..15929)
                     /locus_tag="OO315_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5891755.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(15945..16463)
                     /locus_tag="OO315_002919"
     CDS             complement(15945..16463)
                     /locus_tag="OO315_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5891756.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(16774..16965)
                     /locus_tag="OO315_002920"
     CDS             complement(16774..16965)
                     /locus_tag="OO315_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5891757.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            16983..17135
                     /locus_tag="OO315_002921"
     CDS             16983..17135
                     /locus_tag="OO315_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891758.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            17317..>18451
                     /gene="pdeF"
                     /locus_tag="OO315_002922"
     CDS             17317..>18451
                     /gene="pdeF"
                     /locus_tag="OO315_002922"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5891759.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQ"
BASE COUNT         4361 a         4481 c         4992 g         4617 t
ORIGIN      
        1 ggcacgttac cttgaagcgc tgatcaatta tgaagatcgc tggttctgga agcatccggg
       61 ggttaatcca ttctcggtgg cgcgcgcagc atggcaagat ctcacttcgg gacgggttat
      121 ttccggtggc agcacgctca ctatgcaggt tgctcgtctg cttgatcctc accccaaaac
      181 atttggtggc aaaattctcc agctctggcg cgcgttgcaa ctggagtggc atttgtctaa
      241 gcgtgaaatt ctgatcttgt atcttaaccg cgctccgttt ggcggtacgt tgcaggggat
      301 cggtgcggca agttgggctt atctcggaaa atcgcctgcg aatttaagct attccgaggc
      361 ggcaatgctg gcggtgttac cgcaagtgcc cagccgactg cgcccggatc gttggccgga
      421 gcgtgccgaa gccgcgcgtc gccccactgg catggaaaac gggcaccagc tatggctatc
      481 gtgacgcctg ggcgattggg gttaacgctc gctatgtcat tgggatctgg actggcagac
      541 ctgacggcac gcccgttgtt ggtcagtttg gctttgccag tgccgtacca ttgttgaatc
      601 aggtcaataa tatcttactg tcgcgcagtg cgaatctgcc ggaagacccg cgcccgaact
      661 cagtcactcg tggcgttatc tgctggccgg gcggacagtc tttgccggaa ggtgacggta
      721 actgtcgccg ccgcctggca acctggctgc tggatggcag ccagccgcca acgctgttac
      781 tgtcagagca ggaagggatt aatggcattc gcttccccat ctggctggat gacaaagggc
      841 agcgcgtcgc cgctgactgc ccgcaggcga agcaagaaat ggtaaatgtc tggccgctac
      901 cgctggaacc ctggctgccc gcatcagaac gtcgcgctgt gcgcttacca ccggcctcga
      961 caatttgccc gccatacggt cacgatgccc agctccctct gcagctgaca ggcgttcgcg
     1021 atggcgcgat tattaaacgt ttaccgggcg cagcggaagc gactttgccg ttgcaatcaa
     1081 gtggaggggc aggtgaacgc tggtggtttc tgaatggcga accgttaact gaacgcgggc
     1141 gcaacgtgac cctgcatttg acggataaag gtgattatca attgctggtg atggatgatg
     1201 tggggcaaat cgcgacagtg aaatttgtca tgcaatagtc tgttttgtcc ctgattgttg
     1261 ctaaaactca ttttatttta aaaaaaatgt taccttcatc aatagtcaac ggccctgttg
     1321 ctcattataa tccgcgccat ctcgtacgct ggtacagaca acaacagaac aatttacaga
     1381 ggtaaaaatg gctattgaac gtactttttc catcatcaaa ccgaacgcgg tagcaaaaaa
     1441 cgtcattggt aatatctttg cgcgctttga agctgcaggg ttcaaaattg tcggcaccaa
     1501 aatgctgcat ctgaccgttg aacaggcacg tggcttttat gctgaacacg atggaaaacc
     1561 gttctttgat ggtctggttg aattcatgac ctctggcccg atcgtggttt ccgtgctgga
     1621 aggtgaaaac gccgttcagc gtcaccgcga tctgctgggc gcgaccaatc cggcaaacgc
     1681 actggctggt actctgcgcg ctgattacgc tgacagcctg accgaaaacg gtacccacgg
     1741 ttctgattcc gtcgaatctg ccgctcgcga aatcgcttat ttcttcggcg aaggcgaagt
     1801 gtgcccgcgc acccgttaat aatttcgtaa atgccgcgtg caaacgtggc atccgtgcgc
     1861 cagaatttgt acaatgcagc gcccccggac gagcagccgc tcaccggggc gtttcttttt
     1921 tcaaccctcc aggggccata acgtgtaata acgaggccgg aatagcatat gtctgaacaa
     1981 ttagtcacac ctgaaaacgt caccacgaaa gatggaaaaa tcaacctgct ggatctcaac
     2041 cgtcagcaga tgcgggagtt ttttaaagat ttaggtgaaa aaaccttccg cgccgatcag
     2101 gtgatgaagt ggatgtatca ctattgctgc gacaactttg atgagatgac cgacatcaac
     2161 aaagtgttgc gcggcaaact gaaagaggtg gcggaaatcc gcgcaccgga agtggttgaa
     2221 gaacagcgtt catccgacgg caccattaaa tgggcgatcg ccgttggcga tcagcgcgtc
     2281 gaaacggtgt atatcccgga agacgaccgt gccacgctct gcgtctcttc gcaggtgggg
     2341 tgtgcgctgg agtgtaaatt ctgttccacc gcccagcagg gctttaaccg caacctgcgg
     2401 gtatcggaaa ttattggcca ggtgtggcgt gcggcgaaaa tcgtcggcgc agcaaaagtc
     2461 accggtcagc gcccaatcac taacgtcgtg atgatgggca tgggcgagcc gctgctcaac
     2521 ctgaacaacg tcgttccggc gatggaaatc atgcttgatg atttcggttt tggcctgtct
     2581 aaacgtcgcg tgacgctttc cacttccggt gtagttccgg cgctggataa actgggcgat
     2641 atgatcgacg ttgcactggc aatttccctg cacgcgccga acgacgaaat tcgtgacgaa
     2701 attgtgccga tcaacaaaaa gtacaatatc gaaacgttcc ttgctgcggt gcgccgttat
     2761 ctggagaaat ccaacgccaa tcagggccga gtcactattg aatacgtgat gcttgaccac
     2821 gttaacgacg gcactgaaca cgcgcaccaa ctggcggaac tgctgaaaga tacgccgtgt
     2881 aagatcaacc tgatcccgtg gaacccgttc ccggacgcgc cgtatggacg cagttcgaac
     2941 agccgtatcg accgtttctc aaaagtgctg atgagctacg gtttcaccac tattgtgcgt
     3001 aaaacgcgtg gtgatgatat cgatgctgcc tgtggtcagt tggcgggcga tgttatcgac
     3061 cgtacgaaac gtactctgcg taaacggatg cagggtgaag ccatcgatat taaagcggtc
     3121 tgatagccat tacgccacgg ttactttatg taccgtggcg taatctttta tgaatgaagt
     3181 attaagttgc aattgtctgg tcaatattaa cggtgcgttt ttgctgactt taaggcagta
     3241 tgtaacgatg caacagtaag ttagcctcag tgaatgtggg ctttgtcacg agcacacaga
     3301 cggtcttata ctgtatgata acggttaact taacggatgt ttcgcggtgt gggtgagcat
     3361 gatggttcac cggcatctca attctcattt aaacgtacct gcagcgaatg aatactgaag
     3421 ccacgcacga ccaaaatgaa gcacttacta ccggcgctcg cctgcgtaat gctcgcgaac
     3481 aactaggact tagtcagcag gccgttgccg agcgactttg cctgaaggtt tccacggtac
     3541 gcgacattga agaagataag gcacccgccg atcttgcttc aacattcctg cgcggatata
     3601 tccgctctta tgcgcgtctg gtacatattc cagaagaaga actgctgcca gggctggaaa
     3661 agcaggctcc acttcgggct gcaaaagttg cgccaatgca gagtttttcc ctcggtaaac
     3721 gccgcaaaaa acgcgacggc tggctaatga ccttcacctg gctggtgttg tttgtggtta
     3781 tcggcctgag cggtgcctgg tggtggcaag accacaaagc tcagcaggaa gagatcacca
     3841 ctatggccga tcaatcttcg gcggaactga gcagtaatag cgagcagggg cagagtgttc
     3901 cgttaaatac gtcgacaact acagacccgg ctacaacgtc cacgccgcca gcgtctgtgg
     3961 atactaccgc aaccaacacg caaacacctg ccgtaactgc gccagcacca gctgttgatc
     4021 cgcaacagaa tgcggttgtt tcgccttcgc aggcaaatgt tgataccgcc gcgaccccgg
     4081 taccaacggc agcaacaacg ccagatggtg ctgcgccgtt gccaaccgat caggctggcg
     4141 tgaccacgcc ggcggctgat ccgaatgcgc tggtgatgaa ctttactgcc gattgctggc
     4201 tggaggtcac tgatgctacc ggtaaaaaat tgtttagcgg gatgcagcgt aaagacggta
     4261 atttgaattt aaccggccag gcaccgtata aactgaaaat tggtgcgcca gccgcagtac
     4321 agatccagta tcaagggaaa cctgtcgatc tgagtcgttt tatcagaact aaccaggttg
     4381 cgcgtctgac cctcaatgcc gaacaatcac cggcgcagta acagacgggt aacgcgggag
     4441 atttttcatg cataaccagg ctccaattca acgtagaaaa tcaacacgta tttacgttgg
     4501 gaatgtgccg attggcgatg gtgctcccat cgccgtacag tccatgacca atacgcgtac
     4561 gacagatgtc gaagcaacgg tcaatcaaat caaggcgctg gaacgcgttg gcgctgatat
     4621 cgtccgtgtc tccgtaccga cgatggacgc ggcagaagcg ttcaaactca tcaaacagcg
     4681 ggttaacgtg ccgctggtgg ctgacatcca cttcgactat cgcattgcgc tgaaagtagc
     4741 ggaatacggc gtcgattgtc tgcgtattaa ccctggtaat atcggtaatg aagagcgtat
     4801 tcgcatggtg gttgactgtg cgcgcgataa aaacattccg atccgtattg gcgttaacgc
     4861 cggatcgctg gaaaaagatc tgcaagaaaa gtatggcgaa ccgacgccgc aggcgttgct
     4921 ggaatccgcc atgcgtcatg ttgatcatct cgatcgcctg aacttcgatc agttcaaagt
     4981 cagcgtgaaa gcgtctgacg tcttcctcgc tgttgagtct tatcgtttgc tggcaaaaca
     5041 gatcgatcag ccgctgcatc tggggatcac cgaagccggt ggtgcacgca gcggggcagt
     5101 aaaatccgcc attggtttag gtctgctgct gtcggaaggc atcggcgaca cgctgcgtgt
     5161 atcgctggcg gccgatccgg tcgaagagat caaagtcggt ttcgatattt tgaaatcgct
     5221 gcgtatccgt tcgcgaggga ttaacttcat cgcctgcccg acctgttcgc gtcaggaatt
     5281 tgatgttatc ggtacggtta acgcgctgga gcaacgcctg gaagatatca tcactccgat
     5341 ggacgtttcg attatcggct gcgtggtgaa tggcccaggt gaggcgctgg tttctacact
     5401 cggcgtcacc ggcggcaaca agaaaagcgg cctctatgaa gatggcgtgc gcaaagaccg
     5461 tctggacaac aacgatatga tcgaccagct ggaagcacgc attcgtgcga aagccagtca
     5521 gctggacgaa gcgcgtcgaa ttgacgttca gcaggttgga aaaataataa cgtgatggga
     5581 agcgcctcgc ttcccgtgta tgattgaacc cgcatggctc ccgaaacatt gagggaagcg
     5641 ttgagggttc atttttatat tcagaaagag aataaacgtg gcaaaaaaca ttcaagccat
     5701 tcgcggcatg aacgattacc tgcctggcga aacggccatc tggcagcgca ttgaaggcac
     5761 actgaaaaac gtgctcggca gctacggtta cagtgaaatc cgcttgccga ttgtagagca
     5821 gaccccgcta ttcaaacgtg cgattggtga agtcaccgac gtggttgaaa aagagatgta
     5881 cacctttgag gatcgcaatg gcgacagcct gactctgcgc cctgaaggga cggcgggctg
     5941 tgtacgcgcc ggcatcgagc atggtcttct gtacaatcag gaacagcgtc tgtggtatat
     6001 cgggccgatg ttccgtcacg agcgtccgca gaaagggcgt tatcgtcagt tccatcagtt
     6061 gggctgcgaa gttttcggtc tgcaaggtcc ggatatcgac gctgaactga ttatgctcac
     6121 tgcccgctgg tggcgtgcgc tgggtatctt cgaacacgta actcttgagc tgaattctat
     6181 cggttcgctg gaagcacgcg ccaattaccg cgatgcgctg gtggcattcc ttgagcagca
     6241 taaagaaaag ctggacgaag actgcaaacg ccgcatgtac actaacccgc tgcgcgtgct
     6301 ggattcaaaa aatccggaag tgcaggcgct tctcaacgac gctccggcat taggtgatta
     6361 tctggacgag gaatctcgtg agcattttgc cggtctgtgc aaactgctgg agagcgcggg
     6421 gatcgcttac accgtaaacc agcgtctggt gcgtggtctg gattactata accgtaccgt
     6481 tttcgagtgg gtgactaaca gtctcggctc ccagggcacc gtgtgtgcag gcggtcgtta
     6541 tgacggtctt gtggaacaac tgggcggtcg tgcaacaccg gctgtcggtt ttgcgatggg
     6601 cctcgaacgt cttgtattgt tagtacaggc cgttaatccg gaatttaaag ccgatcctgt
     6661 tgtcgatata tacctggtgg cttcaggtgc tgatacacaa tctgcggcta tggcattagc
     6721 tgagcgtctg cgtgatgaat tactgggcgt gaaattgatg accaaccacg gcggcggcaa
     6781 ctttaagaaa cagtttgccc gtgctgataa atggggtgcc cgcgttgctg tggtgctggg
     6841 tgagtctgaa gtggctaacg gcacagcagt agtgaaggat ttgcgctctg gtgagcaaac
     6901 ggcagttgcg caggatagcg tagccgcgca tttgcgcacg ttactgggtt aaggaaggag
     6961 aaggacagcg tggaaattta cgagaacgaa aacgaccagg tagaagcggt taaacgcttt
     7021 tttgctgaaa atggcaaagc actggctgtt ggggggattt tgggcgttgg cgcactgact
     7081 ggctggcgct actggaacag ccatcaggtt gattctgcac gctccgcttc tcttgcctat
     7141 caaaatgcgg ttaccgcagt gagcgaaggc aaaccggata gcatcccggc ggcggaaaaa
     7201 tttgctgctg aaaataaaaa tacttatggt gcgctggctt ctttggaact tgcgcagcaa
     7261 tttgttgaca aaaatgaact agagaaagct gccgcccagt tacaacaggg gctggcagac
     7321 acgagcgatg aaaatctcaa agccgtgata aatctgcgtc ttgctcgcgt tcaggtacag
     7381 ctcaagcagg ctgatgccgc gctgaaaacc cttgatacca tcaaaggtga agggtgggct
     7441 gccattgttg ccgacttgcg tggtgaagca ttgctgagca aaggtgataa gcaaggtgcg
     7501 cgtagtgcat gggaagcagg cgtgaaaagc gatgttactc cggcactgag cgaaatgatg
     7561 cagatgaaaa ttaataattt gtccatctga gagggacccg atgcaattgc gtaaattact
     7621 gctgccagga ctgctttccg ttaccctttt aagcggctgt tcgctgttta acagcgaaga
     7681 agatgtggta aagatgtccc cattgccaac cgttgaaaac cagtttacgc cgaccacggc
     7741 gtggagcact tccgttggta gcggcattgg caacttctat tccaatcttc atccggcact
     7801 ggcggacaac gttgtctatg cagcggaccg cgctggtttg gtaaaagcgc tgaatgcgga
     7861 tgatggcaaa gaaatctggt ctgtcagcct ggccgagaaa gatggctggt tctctaaaga
     7921 gcctgcatta ctttctggcg gtgtgaccgt gtctggtggg catgtctaca ttggcagcga
     7981 aaaggcgcag gtttacgcgc tgaataccag cgatggtact gtggcatggc aaactaaagt
     8041 cgcgggtgaa gcactttcgc gcccggtggt cagcgacggt ctggtgttaa tccacaccag
     8101 taacggtcag ttacaagcgc tgaacgaagc tgacggcgct gtcaaatgga cagttaacct
     8161 cgatatgcct tcgctctctt tgcgtggcga gtctgcgccg gcaacggctt ttggtgcggc
     8221 cgtcgtgggt ggcgataatg gtcgcgtcag cgcagtgctg atggaacagg gccagatgat
     8281 ttggcagcag cgtatttccc aggcgaccgg ttctaccgaa attgaccgtc tgagcgatgt
     8341 tgacacgact cccgtcgttg ttaacggcgt tgttttcgcg ctggcctata atggtaacct
     8401 gacggcgctt gatctgcgca gtggtcagat tatgtggaaa cgcgaactgg gttcggtgaa
     8461 tgatttcatc gtcgatggca atcgcatcta tctggtcgat caaaatgacc gggtgatggc
     8521 gttgaccatt gatggcggcg ttacgctgtg ggcacaaagc gatctgctgc atcgcctgct
     8581 gacttctccg gtgctgtata atggcaacct ggtggtcggt gacagtgaag gttatctgca
     8641 ctggattaac gtcgaagatg gtcgtttcgt tgcccagcaa aaagttgata gttccggttt
     8701 ccagactgaa ccggttgccg ctgacggcaa actgctgatc caggcaaaag acggaaccgt
     8761 gtactctatt acacgttaat cgtctctgtc gttcactttg aaaacggctc ctggacaggg
     8821 gccgttttcc tgtttttaac aacgacgcga atatagcgtg cgttgtctga tgatttataa
     8881 aaatgaggct ttaaacatgg tacctgtggt cgcgcttgtc gggcgcccta acgtaggaaa
     8941 atccacgtta tttaaccgtc taactcgcac ccgagatgcg ctggttgcgg atttcccggg
     9001 tctgactcgt gaccgtaagt acggtcgtgc ggaaattgaa ggccgtgagt ttatctgtat
     9061 tgataccggc gggattgatg gcacagaaga cggtgtagaa acccgcatgg cggaacagtc
     9121 gctgctggcg attgaagaag cggacgtcgt actgtttatg gtggatgcgc gcgcgggcct
     9181 gatgccggca gatgaagcga ttgccaaaca tctgcgctcc cgtgaaaaac cgaccttcct
     9241 ggtggtaaac aaaactgacg gtctggatcc cgatcaggca gtggttgatt tctactcgct
     9301 tggtttaggt gaaatctacc cgatcgccgc gtctcacggt cgtggcgtat taagtctgct
     9361 ggagcatgtg ctgctgccgt ggatggaaga tctcgcaccg caagaggaag tcgacgaaga
     9421 cgctgaatac tgggcgcaat ttgaagcgga agagaacggc gaagaagaag aggaagacga
     9481 cttcgacccg caaagtctgc cgatcaaact ggcgattgtg ggtcgtccga acgtaggtaa
     9541 gtctacactc actaaccgta ttcttggaga agagcgcgtt gttgtttacg acatgcctgg
     9601 cacgacgcgt gacagcatct acatcccaat ggaacgcgat ggacgtgagt atgtgctcat
     9661 tgacaccgct ggcgtacgta aacgcggcaa aatcaccgat gctgtagaga aattctccgt
     9721 aatcaaaacg ttgcaggcca ttgaagacgc caacgtggtg atgttagtga ttgatgcgcg
     9781 cgaaggtatt tccgatcagg atctctcgct gctgggcttt attctcaata gtgggcgctc
     9841 acttgtcatt gtggtgaata agtgggatgg cctgagccag gaagtgaaag agcaggtgaa
     9901 agaaacgctg gacttccgtc tgggctttat cgattttgct cgtgtgcact ttatctctgc
     9961 cttgcacggc agtggtgttg gtaacttgtt tgaatcagta cgtgaagcgt atgacagctc
    10021 cacccgtcgt gtggggacct ctatgctgac gcgcatcatg acgatggctg ttgaagatca
    10081 ccaaccgccg ctggtacgcg gtcgtcgtgt gaagctgaaa tatgcccacg ctggtggtta
    10141 taacccgccg attgtggtga ttcacggtaa tcaggtgaaa gacctgcctg attcctacaa
    10201 gcgctacttg atgaactact tccgcaaatc gctggacgta atgggatcgc cgattcgtat
    10261 tcagttcaaa gaaggggaaa acccgtatgc gaacaagcgt aacaccctga cgccaaccca
    10321 gatgcgtaaa cgtaagcgtc tgatgaagca catcaagaaa aataaataat tatccgtaag
    10381 tattttatct gccattccct ctatgaatgt agagggaatg gctaccggag gaattcatat
    10441 ggaactgcat tgtccaaaat gtcagcatgt gcttgatcag gataacggcc atgcgcgttg
    10501 cccatcctgc ggggaattta ttgaaatgaa agctctttgt ccagactgcc atcaaccgtt
    10561 gcaggtgtta aaagcctgcg gtgcagtaga ttacttctgt cagcacgggc atggcttgat
    10621 atcgaaaaaa cgcgttgaat tcgtgctggc ttaatgaacc tttttacgcg atttttttac
    10681 cggctggatg ttttttactt cactttctat ccagccgtct tccagacgtg tggttagcat
    10741 ttcacccgtt ttaacttgct tcactttttt cagtacattg ccgtcagtag cagtagtaac
    10801 gctgtatcca cgcgccagcg ttgacagtgg gcttacggct tcgaggtgcg ttactgcatt
    10861 accgaaacgt tcacgcgtgg cgctaagctg tgcgcgcagg atttctgcta aacgatattc
    10921 cagttgctga atgcgcgttt gcgtgcgatg aatcttcggc tgtggatttt gctgattcag
    10981 ccgctgtgtt aatcgctgct gctgttgccc ggcacgctta agctgatttt ccagcgcaaa
    11041 gctcatccgt ttttgcaggc gttcaagcat ggtttgctgg cgtgccagcc ggagctgcgg
    11101 atgctgctgc tgcaagcgat gatgaatctg cgtaaagcga cgtgtacggt tggcgagata
    11161 ataatccatc gccatctcca gccgttgacg ggccgattgc acctggcgca gtaactcttg
    11221 ctgattacgg ctcactactt cagcggcggc agacggcgtt ggcgcacgca gatcggcaac
    11281 aaaatcggca atggtcacat ccgtctcatg cccgacggcg ctgacgaccg gaatgcggct
    11341 ggcaaaaatc gcccgcgcta cgcgttcgtc gttaaaactc cataaatctt ccagcgaacc
    11401 gccgccgcgc ccaacgatca acacgtcgca ctcattgcgc tgattcgcca gttcaatggc
    11461 gcgaacaatt tgccccggcg cgtcatcgcc ctgaacggag gtggggtaga tgatcaccgg
    11521 tagagaagga tcgcgacgtt ttaacacatg caaaatatca tgtagcgcag caccggtttt
    11581 tgaggtgatc acaccaacgc aatgcgcagg ggagggaagt ggttttttgt attgctgatc
    11641 gaacaaacat tcagcctgca acttcgcttt gagctgttcg tacttctgtt gcagcagccc
    11701 ttcaccggcc ggctgcatac tctcaacgat tatctggtag tcgccgcgcg gctcgtagag
    11761 cgtaatattg gcgcgaacta aaacttgttg cccatgctgc ggacggaagg tgacccggcg
    11821 gttgctgttg cggaacatcg cgcagcgtac ctgggcggtg tcgtctttga gtgtaaagta
    11881 ccagtgaccg gaagccggtt gtgtgaaatt agagatttcg ccgctgatcc aaacctgtcc
    11941 catctcatgc tcaagcagca gacgaaccgt ttgattcagg cgactaacgg taaaaattgc
    12001 aggggattga gaaggtaaca tgtgagcgag atcaaattct aaatcagcag gttattcagt
    12061 ctatagtaac ccgcccttcg gggatagcaa gcattttttg caaaaagggg tagatgcaat
    12121 cggttacgct ctgtataatg ccgcggcaat atttattaac cactctggtc gagatattgc
    12181 ccatgctacg tatcgctaaa gaagctctga cgtttgacga cgttctcctc gttcctgctc
    12241 attctaccgt tctgccgaat actgctgacc tcagcaccca gctgacgaaa actattcgtc
    12301 tgaatatccc tatgctttcc gcagcaatgg ataccgtaac ggaagcgcgc ctggctattg
    12361 ctctggctca ggaaggcggt atcggcttta tccacaaaaa catgtccatt gaacgccagg
    12421 cagaagaagt tcgccgtgtg aaaaaacacg aatctggtgt ggtgactgat ccgcagactg
    12481 ttctgccaac cacgacgctg cgcgaagtga aagaactgac cgagcgtaac ggttttgcgg
    12541 gctatccggt cgttaccgaa gaaaacgaac tggtgggtat tatcaccggt cgtgacgtgc
    12601 gttttgttac cgacctgaac cagccggtta gcgtttacat gacgccgaaa gagcgtctgg
    12661 tcaccgtgcg tgaaggtgaa gcccgtgaag tggtgctggc aaaaatgcac gaaaaacgcg
    12721 ttgaaaaagc gctggtggtt gatgacgaat tccacctgat cggcatgatc accgtgaaag
    12781 acttccagaa agcggaacgt aaaccgaacg cttgtaaaga cgagcaaggc cgtctgcgtg
    12841 ttggtgctgc agttggcgca ggtgcgggta acgaagagcg tgttgacgcg ctggttgccg
    12901 ctggcgttga cgttctgctg atcgactcct cccacggtca ctcagaaggc gttttgcagc
    12961 gtatccgtga aacccgtgct aaatatccgg atctgcaaat catcggcggc aacgtggcaa
    13021 cagctgcagg tgcacgcgct ttggcagaag ctggttgcag cgcggttaaa gtcggtatcg
    13081 gccctggttc tatctgtacg actcgtatcg ttactggcgt aggtgttccg cagatcactg
    13141 ccgttgctga cgcagttgaa gccctggaag gcaccggtat tccggttatc gcagatggtg
    13201 gtattcgctt ctccggcgac atcgccaaag ctatcgccgc tggcgcaagc gcggtgatgg
    13261 taggttccat gctggcgggt actgaagaat ctccgggtga aatcgaactc taccagggcc
    13321 gttcttataa atcttaccgt ggtatgggtt ccctgggcgc gatgtccaaa ggttcctctg
    13381 accgttattt ccagagcgat aacgctgccg acaaactggt gccggaaggt atcgaaggtc
    13441 gcgtagctta taaaggtcgc ctgaaagaga tcattcacca gcagatgggc ggcctgcgct
    13501 cctgtatggg tctgaccggc tgtggtacta tcgacgaact gcgtactaaa gcggagtttg
    13561 tacgtatcag cggtgcgggc attcaggaaa gccacgttca cgacgtgacc attactaaag
    13621 agtccccgaa ctaccgtctg ggctcctgat tctcttcgcc cgatttcatg tcgggcgatt
    13681 tatattatct gtttcacttg cctcggaata agcgtcaatg acggaaaaca ttcataagca
    13741 tcgcatcctc attctggact tcggttctca gtacactcaa ctggttgcgc gccgcgtgcg
    13801 tgagctgggt gtttactgcg aactgtgggc gtgggatgtg acagaagcac aaattcgtga
    13861 cttcaatcca agcggaatta ttctttccgg cggcccggaa agtactactg aagaaaacag
    13921 tccgcgtgcg ccgcagtatg tctttgaagc aggcgtaccg atattcggcg tttgctatgg
    13981 catgcagacc atggcaatgc agttgggcgg tcacgttgaa gcctctaacg aacgtgaatt
    14041 tggctacgcg caggtagaag tcgtaaacga cagcgcactg gttcgcggta tcgaagatgc
    14101 gctgaccgca gacggtaaac cgctgctcga tgtctggatg agccacggcg ataaagttac
    14161 cgctatcccg tccgacttcg tcaccgtagc cagcaccgaa agctgcccgt ttgccattat
    14221 ggctaacgaa gaaaaacgct tctatggcgt acagttccac ccggaagtga ctcacacccg
    14281 ccagggtatg cgcatgctgg agcgttttgt gcgtgatatc tgccagtgtg aagccctgtg
    14341 gacgccagcg aaaattatcg acgatgctgt agctcgcatc cgcgagcagg taggcgacga
    14401 taaagtcatc ctcggcctct ctggtggtgt ggattcctcc gtaaccgcaa tgctgctgca
    14461 ccgtgctatc ggtaaaaacc tgacttgcgt attcgtcgac aacggcctgc tgcgtctcaa
    14521 cgaagcagag caggttctgg atatgtttgg cgatcacttt ggtcttaaca ttgttcacgt
    14581 accggcagaa gatcgcttcc tgtcagcgct ggctggcgaa aacgatccgg aagcaaaacg
    14641 taaaatcatc gggcgcgttt tcgttgaagt attcgatgaa gaagcgctga aactggaaga
    14701 cgtgaagtgg ctggcgcagg gcaccatcta ccctgacgtt atcgaatctg cggcttctgc
    14761 aaccggtaaa gcacacgtca tcaaatctca ccacaacgtg ggcggcctgc cgaaagagat
    14821 gaagatgggc ctggttgaac cgctgaaaga gctgttcaaa gacgaagtgc gtaagattgg
    14881 tctggagctg ggcctgccgt acgacatgct gtaccgtcat ccgttcccgg gaccaggcct
    14941 tggcgttcgt gttctgggtg aagtgaagaa agagtactgt gacctgctgc gccgtgctga
    15001 cgccatcttc attgaagaac tgcgtaaagc ggacctgtac gacaaagtca gccaggcgtt
    15061 caccgtcttc ctgccagtac gttccgttgg cgtaatgggc gatggtcgta agtatgactg
    15121 ggttgtctct ctgcgtgctg tcgaaaccat cgactttatg accgcacact gggcgcatct
    15181 gccgtacgat ttcctcggtc gcgtttccaa ccgcattatc aatgaagtga acggtatttc
    15241 ccgcgtggtg tatgacatca gcggcaagcc gccagctacc attgagtggg aatgattttg
    15301 atccctgcac tatgaatgaa caaaaccctc cgttactaca gagggttttt tatcttcaag
    15361 aatcataggc ttgaagttac taacatcgat taattaaacc agctgtccga tttgttctct
    15421 tctgctttgc ccacgctttt catcagatcg cgaccgcctt cagtcatatt tctgtttgcg
    15481 tcagcttcag attgcaccac atcggtttgc gcagctttgt gcttcagttc ctgatcgata
    15541 aattcgtttt cgcgcttaac gcgggcttct tctttcgcca gcgccagttt ttgtttctga
    15601 atctctaagc tgcgtagctc atcttcataa ctttgatcgc gttttttgtc cgcagtggct
    15661 tcggcgtcca gtttatcctg acgagctttc ttatttgccg ctgccgttgc cgctcttttg
    15721 ttcgcggcgg cctgggcatt tgcgcgacgt tgcttctctt gctggatttc cctgttgcgc
    15781 tccgcgaccc attcgtcatg ctgcctttgc tcttcatttt taccttgctg ttccgcttct
    15841 gcgacagcag agagttgatc ctgcaatgat gaggctatag ccggatagct taaggaggct
    15901 aagatggcgc aaagaaaaac tttcttcatg actcctcctg attattagct cttttcagga
    15961 catttggtat ttggctgaat acgcgtttcg ttatacgttg tggtaataac aacggctaaa
    16021 cctgtcgtaa actggcactc tttaccctcc tgggtggagg tatacacttt ggtgccttcc
    16081 ttatatgtta aagaaacacc ttccactaag gttttatcat tcaccataga acccgctgcc
    16141 gcgcctacag ctccgccgcc aactgcccct gccgtcgttc cggaattgct gccagaaccg
    16201 acgttgtggc cgataacacc gccagcgact gcgccaataa gcgcgccgaa ggcttgtgcg
    16261 ttccgtttat tttgggagtt gtctacggca acttttgcgg gaagaatgga aataatatta
    16321 acggttttag tttcttgttt ggtattcagt tgatcggttt gataaacatc ggcggcatga
    16381 tcgtcagcat ttgactggca tcctgccaga gtgaatgacg ctaacattgc cacaggcaga
    16441 agacattttt taaatttcat cactattcct tgttatacac attacgaaaa ataagacatt
    16501 taagatcatc aaaagaggaa agtaaatgta ttgcgtataa agggatagat taaatttatt
    16561 aaaattcacg cagggaataa tttataaaaa tatatcatta caacatatat attaatttta
    16621 tatttatttc gcagccaaac aggataaaca tcagtcagga caaagacgta aaatttaaga
    16681 tgaaagaata tctgccgctt tgataatccc aaagagcaaa agcatccgac agcgaagtgc
    16741 cgttatgttc tgtgaaagtg gagaaggtaa ccgctaacgt tgtaccggtg attcgacgga
    16801 gagtgtggat tgctgagctt cttttttgct ctgatggtgc tgccaggcac cgacggacga
    16861 gtagataaaa cggccaaaga agaagataaa gctgatgagc agtacgatac gggtcatgcg
    16921 actgttaaat cggtgtcgtt ttcgcatact agttgcctga ctcacaaaag gttccttgaa
    16981 gtatgtccca cgctgtggac ggtacttaca ttaaggcaca acaggacaaa atggtcaatt
    17041 cttcgttatg taaaaaagcg tcagtggata catattttaa tgttatggaa gttaatttaa
    17101 ttatttacaa tgatgatgta ataatgatga attaacatga taaacgtaat aattcattaa
    17161 tcagggttat tttgtttgat atatatcaat tggaactttc atatgacact tagaatcaat
    17221 gctctctata gtgatgaata atcatcattc gaagtcaggt gggatgcctg tctgaataca
    17281 cctccttcag gatgtggggg attctgctga gcatctatga aactgaatgc aacttatata
    17341 aaaatacgtg ataaatggtg ggggcttccg ctgttcctgc cttctttaat cttgcccatt
    17401 ttcgcccaca ttaatacttt cgcgcatatt tcttccggtg aggtttttct cttttatctg
    17461 cctttggcac tgatgatcag catgatgatg tttttcagct gggcggcatt gccagggatc
    17521 accttaggga tttttgtccg caaatatgca gagctgggtt tttacgaaac gctctcatta
    17581 acggctaatt ttattatcat tatcattctc tgttggggcg gttacagggt ctttactccc
    17641 cggcgtaaca acgtttcaca tggtgatacc cgtttaattt cccagcgtat attctggcag
    17701 atagtgtttc ctgcaacgct gtttctgata cttttccagt ttgctgcatt tgtaggatta
    17761 ctggcgagca gagaaaatct ggtcggtgtc atgcctttta acctcgggac tttaatcaat
    17821 tatcaggcct tactggtggg taatctgatc ggtgtcccgc tgtgctactt catcattcgg
    17881 gtagtgcgaa atccatttta tttacgtagc tattattcgc aattaaaaca gcaggttgat
    17941 gccaaagtca ccaaaaaaga gttcgcgctc tggctactgg cattaggtgc tttattgttg
    18001 ctgttatgca tgccgttaaa tgaaaaaagc acaattttta gcaccaatta taccttgtca
    18061 ttattgctgc ccctgatgat gtggggagca atgcgctatg gttataagct gatttcgctg
    18121 ctctgggcgg tcgtgttgat gatcagcatc cacagctatc aaaattacat tcccatttat
    18181 cctggctata ccacgcagct gaccataacc tcctccagtt atctggtatt ctcttttatt
    18241 gtcaattata tggctgtact ggcaactcgt cagcgagcgg tagtcagacg cattcagcgg
    18301 cttgcgtatg tggacccggt ggttcatctg ccaaatgttc gcgccctgaa tcgcgcgttg
    18361 cgtgatgccc cctggtctgc gctttgttat ttacgcatcc ctggcatgga aatgctggtt
    18421 aagaactatg gcatcatgct gcggattcaa t
//
LOCUS       DAJTRD010000078        18076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTRD010000078 DAJTRD010000000
VERSION     DAJTRD010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.078"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            256..780
                     /locus_tag="OO315_002923"
     CDS             256..780
                     /locus_tag="OO315_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5891760.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQS
                     GDVLSSLVMRVAQD"
     gene            861..1535
                     /locus_tag="OO315_002924"
     CDS             861..1535
                     /locus_tag="OO315_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5891761.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1551..4031
                     /locus_tag="OO315_002925"
     CDS             1551..4031
                     /locus_tag="OO315_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5891762.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            4047..5080
                     /locus_tag="OO315_002926"
                     /pseudo
     CDS             4047..5080
                     /locus_tag="OO315_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(5162..5500)
                     /gene="rcnB"
                     /locus_tag="OO315_002927"
     CDS             complement(5162..5500)
                     /gene="rcnB"
                     /locus_tag="OO315_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5891763.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5719..6540)
                     /gene="rcnA"
                     /locus_tag="OO315_002928"
     CDS             complement(5719..6540)
                     /gene="rcnA"
                     /locus_tag="OO315_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HCR5891764.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRGERNWLENMHGHDYEHHHHHHDHEHHHDQGHHHHHEHGEYQDAHARAHANDI
                     KRRFDGREVINWQILLFGLTGGFIPCPAAITVLLICIQLKALTLGATLVVSFSIGLAL
                     TLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            6661..6933
                     /gene="rcnR"
                     /locus_tag="OO315_002929"
     CDS             6661..6933
                     /gene="rcnR"
                     /locus_tag="OO315_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5891765.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      7025..7121
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            7156..7944
                     /gene="thiM"
                     /locus_tag="OO315_002930"
     CDS             7156..7944
                     /gene="thiM"
                     /locus_tag="OO315_002930"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5891766.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7941..8741
                     /gene="thiD"
                     /locus_tag="OO315_002931"
     CDS             7941..8741
                     /gene="thiD"
                     /locus_tag="OO315_002931"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5891767.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8806..9627
                     /locus_tag="OO315_002932"
     CDS             8806..9627
                     /locus_tag="OO315_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5891768.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9675..10421
                     /locus_tag="OO315_002933"
     CDS             9675..10421
                     /locus_tag="OO315_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5891769.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(10395..11360)
                     /locus_tag="OO315_002934"
     CDS             complement(10395..11360)
                     /locus_tag="OO315_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5891770.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(11357..12360)
                     /locus_tag="OO315_002935"
                     /pseudo
     CDS             complement(11357..12360)
                     /locus_tag="OO315_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            12400..13452
                     /gene="fbaB"
                     /locus_tag="OO315_002936"
     CDS             12400..13452
                     /gene="fbaB"
                     /locus_tag="OO315_002936"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5891771.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13760..14614
                     /gene="gatY"
                     /locus_tag="OO315_002937"
     CDS             13760..14614
                     /gene="gatY"
                     /locus_tag="OO315_002937"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5891772.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14643..15905
                     /gene="gatZ"
                     /locus_tag="OO315_002938"
     CDS             14643..15905
                     /gene="gatZ"
                     /locus_tag="OO315_002938"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5891773.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKAYVRAGFSKIHLDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGHALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQS
                     GELSAMPHQLIMNKIYDVLRAYRYGCAE"
     gene            15915..16367
                     /gene="gatA"
                     /locus_tag="OO315_002939"
     CDS             15915..16367
                     /gene="gatA"
                     /locus_tag="OO315_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5891774.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            16398..16682
                     /gene="gatB"
                     /locus_tag="OO315_002940"
     CDS             16398..16682
                     /gene="gatB"
                     /locus_tag="OO315_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5891775.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            16686..18041
                     /locus_tag="OO315_002941"
     CDS             16686..18041
                     /locus_tag="OO315_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5891776.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
BASE COUNT         4730 a         4111 c         4573 g         4662 t
ORIGIN      
        1 aatcatttat ataaatgatt tcgtcttttt tattgatatc aacaatacca tttacataaa
       61 tctacattaa ttaatagatg tgataaatat ttaatattct taagtatttg ttgtatttat
      121 attgcattgg ttaatttgtt ttaaatcaat aaaacaatat gtttacattg cggcagcttt
      181 tttgatgtga gaaatttgtt tccaagagat acagactctt aacaaccaaa aataaatttt
      241 attaaggaac atattatgaa acgttcaatt attgctgccg ctgtcttttc ttcttttttt
      301 atgagcgctg gagtatttgc tgcagacgtt gataccggaa cattaaccat taaaggaaat
      361 attgcagaat ctccgtgtaa attcgaagcg ggtggtgatt cagtaagtat taatatgccg
      421 actgtaccaa ccactgtctt tgaaggtaaa gctaaatatt ctacctatga tgatgcagtc
      481 ggtgtaacca gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc
      541 tcgttgatca caaacgacaa aataaccggt aacgataagg cgatagccag tagcaacgat
      601 accgtaggtg ataacagcga tgtcctagat gtttctgcac cttttaacat tgagagttat
      661 aaaacagcgg aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat
      721 aactcagtgc aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa
      781 taaataaaat tgcaagtttc tccaggtaat aaacatggcc gctatcccca tggcggcttt
      841 ttaatttaag aggcattaag atgaaaggat tattatcttt actcattttt tctatggtcc
      901 ttcctgcaca tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata
      961 aagaagttat ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga
     1021 ttgatgatgg cgatacgtcg ttaccaccag aaaaaattca ggttcctttc atgttaacgc
     1081 caccagtggc aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga
     1141 ataaactgcc cactaataaa gaaagcattt tttatctgaa tgttctgtat attccaccaa
     1201 atagtccaga gcaagaaggc aagaatgcac tgaagtttgc gatgcaaaac agaattaagt
     1261 tgttttaccg gccagcgggt attgctccgg taaataaagc gacctttaaa aagttactgg
     1321 taaatcgcag tggcaatgga ttggtgataa aaaatgactc agctaattgg gtgacgattt
     1381 cggatgtcaa agctaataat gttaaagtca attatgaaac tattatgatt gcccccttag
     1441 aaagtcagag tgttaatatc aaaagtaata atgcaaataa ctggtatctg accattatca
     1501 atgaccacgg caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa
     1561 tgaccccact tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg
     1621 aagaaacctt tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata
     1681 ttcgtcttga tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata
     1741 agcaatggcg cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat
     1801 caagagaagt tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat
     1861 gtttaacatt tgagcaactt gttcagggtg ggagctatag ctgggatatc ggggtttttc
     1921 gtctcgattt cagtgtcccg caggcttggg tggaagaact ggaaagtggc tatgttccac
     1981 cggaaaactg ggagcggggt attaatgcgt tttatacttc ttattatgtg agtcagtatt
     2041 acagcgacta taaagcgtcg ggtaacaaca agagtacata tgtacgtttt aacagcgggt
     2101 taaatttact ggagtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc
     2161 caggggtgtg gaaaagcaat accctgtatc tggaacgtgg atttgcccaa tttctcggca
     2221 cgcttcgcgt gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg
     2281 gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac
     2341 gggtgcaggg gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg
     2401 tttatcagaa agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg
     2461 gtggagcaga tcttgatgtt agcgtgaaag aggcggacgg ctcggtaacc acctatctgg
     2521 tgccttatgc agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gattttgcgg
     2581 cgggccgtag ccatattgaa ggggcgagca agcaaagtga ttttgtccag gcaggttatc
     2641 agtatggttt taataactta ttgacgctgt atggcggcac gatggttgct aataattact
     2701 atgcgtttac cctcggaacg ggttggaaca cgcgcattgg cgcaatttcc gtcgatgcca
     2761 cgaagtcgca tagtaaacaa gacaacggcg atgtgtttga cgggcaaagt tatcaaattg
     2821 cctacaacaa atttgtgagc caaacgtcga cgcgttttgg tctggcggcc tggcgttatt
     2881 cgtcgcgtga ttaccggaca tttaacgatc acgtttgggc aaacaataaa gataattatc
     2941 gccgtgatga aaacgatatc tatgacattg ccgattatta ccagaacgat tttggccgta
     3001 aaaatagctt ttctgccaat atgagccagt cattgccaga aggctggggt tctgtgtcct
     3061 taagtacgtt atggcgagat tactgggggc gtagcggcag cagtaaggat tatcagttga
     3121 gttattccaa taactggcgg cggataagct ataccctcgc ggcaagccag gcgtatggcg
     3181 agaatcatca tgaagagaaa cgttttaata tttttatatc aattccctgt gattggggtg
     3241 atgacgttac gacgcctcgt cggcaaatat atatgtctaa ctcaacgacg tttgatgatc
     3301 agggttttgc ctcaaataat acgggattat caggaacagt agggagtcgg gatcagttca
     3361 attatggtgt caacctgagt catcaacatc agggaaatga aacgacagct ggggcgaatt
     3421 tgacctggaa cgcgccggtt gcgacagtga atggcagtta tagtcagtcg agtacttatc
     3481 gacagactgg agccagtgtt tcagggggca ttgtcgcctg gtcgggtggc gttaatctgg
     3541 cgaaccgtct ttccgaaacg tttgctgtga tgaatgcgcc aggaattaaa gatgcttatg
     3601 tcaatgggca aaaatatcgc acaacaaacc gtaatggagt ggtggtatac gacggaatga
     3661 caccttatcg ggaaaatcac ctgatgctgg atgtgtcgca aagcgatagc gaagcagaat
     3721 tacgtggcaa ccggaaaatt gccgcccctt atcgcggcgc ggttgtactg gttaattttg
     3781 ataccgatca gcgcaagccc tggtttataa aagcgttaag agcggatggt caaccattaa
     3841 cgtttggtta tgaagtcaat gatatccatg gtcataatat tggcgttgtc ggccagggaa
     3901 gccagttatt tattcgcacc aatgaaatac cgccatcggt taatgtagca attgataagc
     3961 aacaaggact ttcatgcaca atcaccttcg gtaaagagat tgatgaaagt agaaattata
     4021 tttgccagta atagtcaggt ggttctatgg aaattcgcat aatgctattt atattaatga
     4081 tgatggttat gcctgtgagc tatgcggcat gttatagtga gttatctgtt cagcacaatt
     4141 tggttgttca ggggaatttt gcacttactc aaacacaaat agcgacatat gagcataatt
     4201 ttaatgattc gtcatgcgtg agtacaaata ctatcacccc tatgagccca tcggatatta
     4261 ttgttggact ttataacgat accattaaat taaatttaca ttttgaatgg accaataaaa
     4321 acaacatcac gttgtcaaat aatcagagca gtttcaccag tggttattcg gtaacggtga
     4381 cacctgcggc cagtaatgca aaagtgaata tttctgcggg gggcggtggt tcagtgatga
     4441 ttaatggtgt tgcgacatta tccagtgctt catcatcgac acgcgggagt gccgcagtac
     4501 aatttctact gtgtttatta ggtggcaagt catggggtgc atgtgtaaat agctacagaa
     4561 atgcattggc acaaaatgca ggtgtctatt ccttcaatct gacattgtca tacaacccga
     4621 taaccacaac ctgcaaacag gacgatttat taattacttt agacagtatt cccgttttac
     4681 aattaccagc cacaggtaac aaagcaacaa taaatagtaa aaaaagggat attattctgc
     4741 gttgtaaaaa tttattaggt caacaaaatc aaacatcacg gaaaatgcag gtgtatttat
     4801 caagttctga cttgttaacc aacagcaaca caatactgaa aggtgcggaa gataatgcgt
     4861 aggatttatt cttgaaagta atggttcgcc agtcacactt ttaaatatca ctaacagcag
     4921 taaaggatac acaaatttaa aagaaattgc ggcaaagtca aaacttacag atacaacggt
     4981 ttcaattccg ataacagcca gttactacgt ctacgataca aacaaagtta aatctggcgc
     5041 actggaggca accgcattaa tcaacgtgaa atacgactaa taacaagcaa aacgagcaag
     5101 tggctaataa taaaaaataa caaggtgtct gcatttcccc ctcatgatga gggggctttt
     5161 tttagcgatg ataaaaaatc tcaccgtcgt aggctttaat gattttaccg tcggtgtcgc
     5221 tgatcaacac gtacgcgcca cccatatagg tccagtgcgt cccggcatca ggcgcgggca
     5281 ggttacgtaa ctgccactgc ttaatgttgt actcatcggt gcggtacatc tctgggacgg
     5341 tatcacctgg tttgaaatgc ttaaagtcag caatgaaact acttaactca tacttagaaa
     5401 ttcccgaggt attggtcgaa cccgcggtgg ctggtgcggc ccaggcggca ctggtaacca
     5461 gcaaaagcac acccagcaac atcttatttt taatggtcat aaactctcca ctttggggat
     5521 ctgtaatatc agtcgcatat ctttcctgtc atacaatata actgacaagc gatattttgt
     5581 tagtgatccc atgatttcgt aaaaagtgta aatgaaagga tgccctaaaa gcatccttag
     5641 cgagcagaga gtcagcggaa atagctgatt taagaatcat cctgggctga agataacatg
     5701 caagcctaaa ggattgagtt atcgcattat gcccatgaag ccatgtacgc ccatatacac
     5761 accgactaag ccaatcaaca gactggaaaa atagggggcg cgtttagcga gagtgttaaa
     5821 tccgctccag cgttttgcga cctgctgaac gctgattgct gcgccaacgc ctacggtgac
     5881 aagcgttaac gccaggccaa tgctgaaact gacgaccagt gttgcgccca gtgtcagggc
     5941 tttcaactga atgcatatca acagcacggt aattgctgcc gggcagggga taaagccacc
     6001 ggttaagcca aataacaaaa tttgccagtt gatgacctct ctaccatcaa agcgtcgttt
     6061 aatgtcattg gcatgggctc gtgcatgggc atcctgatac tcgccatgtt cgtgatggtg
     6121 atgatgtcct tggtcgtggt gatgttcgtg atcgtgatga tggtgatgat gctcataatc
     6181 atgcccgtgc atattctcca gccagttgcg ttcgccgcgc cagaacatcc agaacgcggt
     6241 gccaataatg atcactgcgg aaatcagctg gagccacggt tctgctgatt gagcagtaaa
     6301 gcgcttactg atcaccatcc cgccaaaggc aatcaaccag accaccgcgg tatgcgaaat
     6361 ggttgctgcc agtccgagca tcaccgcctg tttaatggtg cctttgatgg cgatgataaa
     6421 cgccgccatc atcgtttttg agtgccctgg ttccaggcca tgaagcgcac caagtaagat
     6481 ggcgctgggg atgaagaacc aggcgtttcc ttgctgaaga agagttgtaa attcggtcat
     6541 gataataatt cttagtatta attcggcaat ctgattctac tcccccccag tacctgatac
     6601 taccccccag tagattaata gtgctatgat ttttcatatt cttgttaacc aggtgttgcc
     6661 atgtctcata caatccgtga taaacagaaa ctgaaagcgc gtgccagtaa gattcagggc
     6721 caggtcgtgg cgctcaagaa aatgctcgac gagccgcacg aatgcgctgc agttttacaa
     6781 cagattgctg ctatccgtgg agcggtaaac ggtctgatgc gggaagtgat taaaggtcat
     6841 ctgacggaac acatcgttca ccagggggat gagctaaaac gtgaagaaga tctggatgtc
     6901 gttctgaagg tgctggattc atatatcaaa taatttatta acgcgattgt aaaactgccg
     6961 tttttcctcg tttacaacgg gggcgctgga cattaccatc ctcctctgcg atttatcatc
     7021 gcaaccaaac gactcggggt gcccttctgc gtgaaggctg agaaataccc gtatcacctg
     7081 atctggataa tgccagcgta gggaagtcac ggaccaccag gtcattgctt cttcacgtta
     7141 tggcaggagc aaactatgca agtcgacctg ctgagttcag cgcaatctgc gcacgcgtta
     7201 cacctttttc accaacattc ccctcttgtg cactgcatga ccaatgatgt ggtgcaaacc
     7261 tttaccgcca ataccttgct ggcgctcggt gcatcgccag cgatggttat cgaaaccgaa
     7321 gaggccagtc agtttgcggc tatcgccagt gccttgttga ttaacgttgg cacactgacg
     7381 cagccacgcg cacaggcgat gcgtgctgcc gttgagcaag caaaaagctc tcaaacacgc
     7441 tggacgcttg atccagtagc ggttggtgcg ctcgattatc gccgccattt ttgtcatgaa
     7501 cttttatctt ttaaaccggc agcgatacgt ggtaatgctt cggaaatcat ggcattagct
     7561 ggcattgcta atggcggacg gggagtggat accactgacg ccgcagctaa cgcgataccc
     7621 gctgcacaaa cactggcacg ggaaactggc gcaatcgtcg tggtcactgg cgaggtggat
     7681 tatgttaccg atggacatcg tatcattggt attcacggtg gcgatccgtt aatgaccaaa
     7741 gtggtaggaa ctggctgtgc attatcggcg gttgtcgctg cctgctgtgc gttaccaggc
     7801 gatacgctgg aaaatgtcgc atctgcctgt cactggatga aacaagccgg agaacgcgca
     7861 gtcgccagaa gcgaggggcc aggcagtttt gttccacatt tccttgatgc gctctggcaa
     7921 ttgacgcagg aggtgcaggc atgaaacgaa ttaacgctct gacgattgcc ggtactgatc
     7981 cgagtggtgg tgcggggatt caggccgatc ttaaaacctt ctcggcactt ggcgcttatg
     8041 gttgctcagt tattactgca ctggtggcgc aaaatacccg tggcgtacag tcggtgtatc
     8101 gcattgagcc tgattttgtc gccgcccagc tcgattcggt gttcagcgat gtgcgaatcg
     8161 ataccactaa aatcggtatg ttggcggaaa ccgatattgt tgaagcggtg gcagaacggt
     8221 tgcaacgtta tcagatccaa aacgtggtac tcgacaccgt tatgctggca aaaagcggcg
     8281 acccgctgct ttcaccttcg gcggttgcta cgctgcgcag tcgattattg ccacaggttt
     8341 cattaataac gccaaacttg cccgaagctg ccgccttgct cgacgcgcca cacgcgcgca
     8401 ccgaacagga aatgctggaa caagggcgat cgctgttggc gatgggctgt ggcgcagtgc
     8461 taatgaaagg tggtcatctg gatgatgagc aaagcccgga ctggctgttt acccgcgagg
     8521 gtgaacaacg gtttaccgca ccgcgcatta tgaccaaaaa cacccacggc actggttgta
     8581 cactctctgc ggcgttggct gcactacgcc cgcgccatac aaactgggct gacaccgtac
     8641 aggaggcaaa aatctggctt tcatcggcgt tagcccaggc cgacacgctg gaagttggtc
     8701 acggtattgg tccggttcac cacttccacg cctggtggtg agcaccgtac tatagctgct
     8761 ttggttattg tcagcctttg gcactgacat tcagggatga aaccaatgca gctaagaatt
     8821 accagtcgaa aaaaactcac cgctctatta tgcgctcttg ggctaatttc catcgttgca
     8881 atttatccgc gtcagacggt gaattttttc tattcgacag cagttcagat tacagactac
     8941 atccacttct acggttatcg cccggttaaa tctttcgcta ttcgcattcc tgccagttac
     9001 accattcacg gattagatgt ttcgcgctgg caggagcgta tcgactggca gcgggtggca
     9061 aaaatgcgcg acaacgatat ccgcttacag tttgctttta ttaaggctac ggaaggcgaa
     9121 aagctggtgg acccctattt ttcgcgtaac tggcaattaa gccgcgaaaa tggcctgctg
     9181 cgcggggcgt atcattattt ctccccgtcg gtatccgctt cagttcaggc gcgattattt
     9241 ctgcaaacgg tggatttctc acaaggcgat ttccctgccg tgctggacgt agaagaacgg
     9301 ggaaaattat cggcaaaaga attacgcaag cgggtaagtc agtggctaaa aatggtcgaa
     9361 aaacgcacgg gaaaaaagcc gattatttac tcaggagccg ttttttatca caccaatctg
     9421 gcgggctatt tcaatgaata tccgtggtgg gtggctcact attatcaacg tcgtccggac
     9481 aatgacggca tggcctggcg cttctggcag cattccgacc gtggacaggt agatggcatc
     9541 aatggtccgg tggattttaa tgtgtttaat ggcacgggga tgagttgcag gcattcgttg
     9601 atgggattaa agaaacgcct taaataagaa gcagtgatat actcccacca gactccctgg
     9661 caggaaaaag acaaatggaa caagcgcata cccagctgat tgcccaactg aacgagcgta
     9721 ttttagcggc ggataacacg ccgctttata ttaagtttgc cgaaacggta aaaaatgccg
     9781 tgcgcagcgg ggtgctggag catggcaata ttttgcccgg tgagcgtgat ttaagtcagt
     9841 taaccggcgt ttcgcgcatt acggtgcgca aggcgatgca ggcgctggaa gaagagggcg
     9901 tggtgacgcg ttcgcgcggt tacggcacgc agatcaacaa catcttcgaa tactcgttga
     9961 aagaagcgag aggtttttct cagcaggtgg tattgcgcgg gaaaaagccc gatacgctat
    10021 gggttaataa gcgtgtcgtg aaatgtcccg aagaagttgc gcagcagctg gcggtcgaag
    10081 cgggaagtga tgtctttttg cttaagcgta ttcgctatgt cgatgaagaa gcggtatcga
    10141 ttgaggaatc gtgggttccg gcgcatttaa ttcatgatgt tgatgccatc ggaatttctc
    10201 tttatgacta tttccgcagc cagcacattt acccacaacg tacgcgttcc cgcgttagcg
    10261 cccggatgcc ggatgccgag tttcagtcac atattcagtt agatagcaaa ataccggtgc
    10321 tggtgatcaa gcaagtggcg cttgaccaac agcaacggcc tattgagtac agcattagcc
    10381 actgtcgcag cgatctatac gtttttgtgt gcgaggagta gttcctcgcg cgttggcgca
    10441 caatcaccgc cccgatgccc gacaacccac gacgccactg cattgcccag taatacggca
    10501 tccgccagtg gcagaccaga ggccagcccg gcaagtacgc cacctgcatg actgtcgccc
    10561 gccccaatgg tgtctacaac ttgcgttgga aatgccggaa tgcagccaga agcgtcgttg
    10621 ctgaaatacc atgcgccttc tttatcgaga cgaacgatca acggcgcggc aaatttctcc
    10681 tgccattgct tgccaagtgt tgttatctct gcggataaag caaaacgttc ggcggcaatc
    10741 tcagcctctt gacgattgag tgacactaaa ggtcgacagg ccatgatccg cgccagtaat
    10801 gcgtccggga tatcgccaat acgtgggcaa aaatcgataa acggcgtcac gtcttgcagc
    10861 ttttccagcc attccactaa caattcgccg cagggcgagg ccagttgata accggaaaaa
    10921 tagagcaggt tgccaggcgc aacggttaat cgtgccaacc attggcgatt ccattgattt
    10981 tccacaccgc tgaatgacat aaaagtgcgt tcgccatccg gctcaaccag tgccagacac
    11041 cagccgttat cgctttcggc gttatcgatc aggctgatta acccctcttt cgccatccgg
    11101 ttgcgaataa tctccgccca cacgccctga ccgagcggca aggcattacc cgcttcgatg
    11161 ccgaggcgct ttaacgccac ggcaatattc agtgcgcagc cgccaacgtt aacgctctgc
    11221 tgtttcagtt cgatatcgca cccacgccag gggagggcat aagcgtcggc gatcacgtca
    11281 atgaccgccg cgccgacgac catcaccggc tgacgcgtgg ttaattcagg cagcagtgtg
    11341 tgtaatcgag cgccgctcat accgcctccc gttgttgacg atatttcgcc agcgctgtgg
    11401 catagcggtt gaaatcaagc tgatttaccg catccagctc cgcctttaac gcaggatcga
    11461 cagcgttaat gccatgcaac gcgccgcaaa tcgccgtcgc catagcacct atggtgtctg
    11521 tgtcgccgcc aagattggcg cacaggacgg cgcagcggtt tgggtcggtt tgtgccagtt
    11581 caactagcgc aatggcgcac ggaacggact caatagtgct ggtgcctgcg ccaacgacct
    11641 ggtaaagctg ttcgctggcg gattcggtgc cgtcggcatt acgcacaatt ttcagcgcaa
    11701 tctccagacg tgccgccagt gaggcgctga acgtggtggt gcgtttttgc tgtgcatgtc
    11761 gcgcaattga aggcaatgaa tcaacaatcg ctgaccagcg ttctccgtca atggcacgag
    11821 aaatcgccca tgcgatgact accgcgcctg caaccgccag atcggattta tgggtcgggc
    11881 tggacgccag cgccacatca tcaataaagg aatcaacatc acgcgctgga agcaaacaac
    11941 ctaatggcga gacgcgcatc gctgcgccgt tggttacgcc gttgttttcc agttcagcaa
    12001 cgggtttacc gtcgcgaatg gcgttaagcg caatcttcga ggtcggacct agtacgtttt
    12061 tgttaaaggc gtcgaaacgc aacgcccagt cgagaatatt acgcccaatc agattcggat
    12121 cgatcttgcc ttcacattcc agtaacgcat ccgccagaca caggccatcg aggtatcgtc
    12181 ggtgaattcg gcgcggttaa aataacaggc cgcgttattc tcctttggtc caggcagaaa
    12241 acggtcaatc cagccaaagt gtgctttaac gcggctgcgt ggccaaagct cggagggcat
    12301 ccccatcgca tcccctaacg cctgcccata aagagcaccg agaatacgtt ctgttttcat
    12361 ttaacttccc cttgaatcgg aatttacggg agcatagtaa tgacagatat tgcgcagttg
    12421 cttggcaaag acgccgacaa ccttttacag caccgttgta tgactattcc ttctgaccag
    12481 ctttatctcc ccggacatga ctacgtagac cgcgtgatga ttgacaataa tcgcccgcca
    12541 gcggtgttac gtaatatgca gacgttgtac aacaccgggc gtctggctgg cacaggatat
    12601 ctttctattc tgccggttga ccagggcgtt gagcactctg ccggagcttc atttgctgct
    12661 aacccgctct actttgaccc gaaaaacatt gttgaactgg cgatcgaagc gggctgtaac
    12721 tgtgtggcat caacttacgg cgtgttggtg tcggtatcgc ggcgctatgc gcatcgcatt
    12781 ccatttctcg tcaaacttaa tcacaacgag acgctaagtt acccgaatac ctacgatcaa
    12841 acgctgtatg ccagcgtgga gcaggccttc aacatgggcg cggtggcggt tggtgcgact
    12901 atctattttg gctcggaaga gtcacgtcgc cagattgaag aaatttctgc ggcctttgaa
    12961 cgcgcgcacg agctgggcat ggtgacagtg ctgtgggcct atttgcgaaa ctccgccttt
    13021 aagaaagatg gcgttgatta ccatgtttcc gccgacctga ctggtcaggc gaaccatctg
    13081 gcggcaacca taggtgcaga tatcgtcaaa caaaaaatgg cggaaaataa cggcggctat
    13141 aaagcgatta attacggtta taccgacgat cgcgtgtaca gcaagttaac cagcgaaaac
    13201 ccgattgatc tggtgcgtta tcagttagct aactgctata tgggccgggc tgggttgata
    13261 aactccggcg gtgctgcagg cggtgaaact gacctcagcg atgcagtgcg tactgcggtt
    13321 atcaacaaac gcgcaggcgg aatggggctg attcttggac gtaaggcgtt caagaaatcg
    13381 atggctgacg gcgtgaaact gattaatgcc gtgcaggacg tttatctcga tagcaaaatt
    13441 actattgcct gatacgcctc atacgaacag caccaccccg taggacggat aagacgttta
    13501 cactgcatcc ggcaacctat gcctgatgcg acgcggaagc gtcttatcat gcctacatag
    13561 cactgccacg tatgtttaca ccgcatccgg cataaaaaca cgcgcacttt gctacggctt
    13621 ccctatcggg aggccgtttt tttgcctttc actcctcgaa caattttcat attgttgttt
    13681 ttgcgatcat tatctcgaca tttaaaagca aataatttca ttatattttg aaatcgaaag
    13741 caaacgacaa gatatgaaaa tgtacgtggt atcgacaaag cagatgctga acaacgcaca
    13801 gcgcggcggt tatgcggttc cggcattcaa tattcacaat ctcgaaacga tgcaagtggt
    13861 ggtggaaacc gctgccaacc tgcatgcgcc ggtcatcatc gccggaacgc ctggcacatt
    13921 tactcatgct ggtacagaaa atctgttggc gctggtcagc gcgatggcga agcaatatca
    13981 ccatccactg gcaattcatc tcgaccatca cacgaaattt gacgatatcg ctcagaaggt
    14041 tcgttctggc gtgcgctcag tcatgattga cgcctcgcat ttgccttttg cgcaaaatat
    14101 ttcacgggtc aaagaggtgg tggatttttg ccatcgcttt gatgtcagcg tcgaggcgga
    14161 gctggggcaa cttggcggcc aggaagatga tgtgcaagtc aatgaagtcg atgcgttgta
    14221 caccaacccc gcacaggcgc gtgaatttgc cgaggcaacc gggattgatt ccctggcggt
    14281 cgccatcggc acggctcacg gtatgtatgc cagcgcaccg gtgcttgatt tttctcgact
    14341 ggagaacatt cgccagtggg tgaacttacc gctggtgctg catggcgcgt caggattatc
    14401 gactaaggat attcagcaaa ccatcaaact ggggatatgc aaaatcaacg ttgcaacgga
    14461 gctgaaaaat gccttcttgc agtcgttaaa aaattacctg accgagcacc ccgaagcgac
    14521 cgatccccgg gattatttgc agtcggctaa atccgcaatg cgcgatgtgg tgagcaaagt
    14581 gattgccgat tgtggctgcg agggcagggc ataacgcacc tgccatttaa caaggaaaaa
    14641 acatgaaaac gttaattgcc cggcataaag ctggtgaaca tatcggcata tgttcagtct
    14701 gttctgccca tccgttggtt atcgaagcgg cgctggcatt tgatcgcaac agcacgcgca
    14761 aagtgctgat tgaagcaacg tcaaaccagg tcaatcaatt tggcggttat accggaatga
    14821 caccggcaga ctttcgcgaa tttgttttta cgattgccga taaagttggg tttgcacgcg
    14881 aacgcattat tctcggcggc gatcatctgg gaccaaactg ctggcagcaa gaaaatgcgg
    14941 atgcggcgat ggaaaaatcc gtcgagctgg taaaagcata tgttcgtgct ggcttcagta
    15001 aaattcatct tgatgcgtca atgtcctgcg cgggggatac cataccgtta gcaccagaaa
    15061 cggttgcgga acgagctgct gtgctttgct ttgctgcgga aagtgtggcg acagattgcc
    15121 agcgtgagca actgagctat gtcattggca ctgaagttcc ggttccgggc ggtgaggcca
    15181 gcgccattca gtcagtacac atcacccatg ttgaagatgc cgccaatact ttacgtacgc
    15241 atcaaaaggc ctttattgcc cgtgggctga cagaggcgtt aacacgtgtg attgccatcg
    15301 tggtgcagcc gggtgtggaa tttgatcaca gcaatattat ccattatcag ccgcaggaag
    15361 cgcaggcgct ggcgcaatgg atagaaaaca cccgaatggt ttatgaagca cattctaccg
    15421 attaccagac ccggacggct tattgggaat tagtccgcga tcactttgca atattgaaag
    15481 tcggtcacgc attaaccttt gctttacgcg aggcgatatt tgcactggca caaattgagc
    15541 aggaacttat cgcccccgaa aatcgcagcg gttgcctggc ggtaattgaa gaagtgatgc
    15601 tcgacgaacc gcaatactgg aaaaaatatt atcgcacggg ttttaacgat tcattactgg
    15661 atattcgtta cagcctgtcg gatcgtattc gttattactg gccgcatagt cggattaaaa
    15721 atagcgttga aacgatgatg gtgaatctgc aaggcgtgga catcccactg ggcatgatta
    15781 gtcagtatct tcccaaacaa tttgaacgca ttcagtccgg ggaattatca gcaatgccgc
    15841 atcagcttat tatgaataaa atttatgatg ttttgcgcgc ctatcgctac ggctgtgcgg
    15901 aataaggacg gtatatgact aacctgtttg ttcgtagcgg aatttctttt gtcgatcgta
    15961 gcgaagtttt aacctatatc ggtaatgaga tgctcgcgaa aggtgtggtt catgatacct
    16021 ggccacaggc attaattgcc agagaagcag aattccctac cgggataatg cttgagcagc
    16081 acgccattgc aataccgcat tgtgaggcga ttcatgctaa gtcgtcagcc atttatctgt
    16141 taaggccaac aaataaagtt cattttcagc aagcggatga tgataacgac gtggcggtat
    16201 cgttggttat tgcgttgatt gtggaaaatc cgcagcagca attgaaactt ttacgctgtt
    16261 tatttggcaa gttacaacag cccgatatcg tcgagacact aattactctt cctgaaactc
    16321 agttaaagga atacttcaca aagtatgttt tagattcaga cgaataaatc cctctgtaac
    16381 aataaaaagg actatttatg aaacgcaaga ttattgtcgc ttgcggaggc gcggttgcga
    16441 cctctacgat ggcggcggaa gaaattaaag agttgtgtca gagtcataat attcctgttg
    16501 aattaatcca gtgtcgggtt aatgaaatag aaacctatat ggatggtgtg catttgatat
    16561 gtaccactgc cagggtggat cgtagttttg gcgatattcc gttagttcac ggcatgcctt
    16621 ttgtttctgg tgtcggtatc gaagcattac aaaataaaat tctgactatc ttacagggat
    16681 gacctatgtt ttcagaagtc atgcgttata ttctcgacct cggccctaca gtgatgctgc
    16741 cggttgtcat cattattttt tctaaaatat taggcatgaa ggcaggcgat tgctttaaag
    16801 cgggtctgca tatcgggatt ggctttgttg gcattggcct tgtgattggc ttaatgctgg
    16861 attccattgg tccggcggcg aaagcgatgg cggaaaattt cgacctgaat ctgcatgtgg
    16921 tcgatgtcgg ctggccgggc tcttcaccaa tgacctgggc gtcgcaaatt gcgctggtgg
    16981 cgattccgat tgcgatattg gttaacgtgg cgatgttact gacccgtatg acgcgggtgg
    17041 taaatgttga tatctggaat atctggcata tgaccttcac cggcgcgttg ctgcacctgg
    17101 caaccggttc atggatgata gggatggcgg gtgtggtaat tcacgcggcg tttgtttata
    17161 agctcggcga ctggtttgcc cgcgataccc gaaatttctt tgagctggaa ggtattgcta
    17221 ttccgcacgg tacgtcggcg tatatggggc cgattgcggt gctggtcgat gctatcatcg
    17281 agaaaatccc aggcgttaac cgaattaaat ttagcgccga cgatattcag cgcaaatttg
    17341 gcccttttgg cgaacctgtc accgtgggtt ttgtgatggg gctgattatc ggcatcctcg
    17401 cgggttacga tgtcaaaggt gtattgcagc tggcggtaaa aacggcggca gttatgctgc
    17461 tgatgccacg gatgattaaa cccatcatgg atggtttaac gcccatcgct aagcaggccc
    17521 gtagtcgttt acaggcgaag ttcggcggtc aggagttcct gattggcctg gatcccgcat
    17581 tactgctggg gcatacggcg gtggtatcgg caagcctgat ttttattccg ctcaccattt
    17641 taattgctgt ttgtgttccg ggtaatcagg tactgccgtt tggcgatctt gccactatcg
    17701 gcttcttcgt ggcgatggcg gtcgcggtgc atcgtggaaa tctgttccgc accttaatct
    17761 cgggtgtcat cattatgagc atcaccctgt ggatcgcgac gcaaactatt ggtttgcaca
    17821 cccaaccggc ggctaatgct ggggcgttaa aagccggggg tatggtggct tcaatggatc
    17881 agggcggttc tcccattacc tggttactga ttcaggtttt ctccccgcaa aatattcccg
    17941 gtttcattat tatcggcgca atttatctga ccggtatttt catgacctgg cgtagagcgc
    18001 gtggttttac taaacaagag aaagccgttc tcgcagaata atttttacct gagggggagg
    18061 ttatccccct taacaa
//
LOCUS       DAJTRD010000079        18050 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTRD010000079 DAJTRD010000000
VERSION     DAJTRD010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18050
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.079"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            446..1129
                     /locus_tag="OO315_002942"
     CDS             446..1129
                     /locus_tag="OO315_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5891777.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1173..5835
                     /locus_tag="OO315_002943"
                     /pseudo
     CDS             1173..5835
                     /locus_tag="OO315_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6203..7858
                     /gene="lldP"
                     /locus_tag="OO315_002944"
     CDS             6203..7858
                     /gene="lldP"
                     /locus_tag="OO315_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5891778.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7858..8634
                     /gene="lldR"
                     /locus_tag="OO315_002945"
     CDS             7858..8634
                     /gene="lldR"
                     /locus_tag="OO315_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5891779.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8631..9821
                     /gene="lldD"
                     /locus_tag="OO315_002946"
     CDS             8631..9821
                     /gene="lldD"
                     /locus_tag="OO315_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5891780.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10017..10490
                     /gene="trmL"
                     /locus_tag="OO315_002947"
     CDS             10017..10490
                     /gene="trmL"
                     /locus_tag="OO315_002947"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5891781.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO315_002948"
     CDS             complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO315_002948"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5891782.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO315_002949"
     CDS             complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO315_002949"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5891783.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO315_002950"
     CDS             complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO315_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5891784.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO315_002951"
     CDS             complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO315_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5891785.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13386..13817)
                     /locus_tag="OO315_002952"
     CDS             complement(13386..13817)
                     /locus_tag="OO315_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5891786.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14062..15606
                     /gene="gpmM"
                     /locus_tag="OO315_002953"
                     /gene_synonym="pgmI"
     CDS             14062..15606
                     /gene="gpmM"
                     /locus_tag="OO315_002953"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5891787.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15616..16899
                     /gene="envC"
                     /locus_tag="OO315_002954"
     CDS             15616..16899
                     /gene="envC"
                     /locus_tag="OO315_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5891788.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16903..17862
                     /locus_tag="OO315_002955"
     CDS             16903..17862
                     /locus_tag="OO315_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5891789.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17849..18050)
                     /locus_tag="OO315_002956"
                     /pseudo
     CDS             complement(17849..18050)
                     /locus_tag="OO315_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4547 a         4663 c         4674 g         4166 t
ORIGIN      
        1 aattcactcc ctttacttcg atttaccgct ggttgatatt ctgaaaatgc atttttaccg
       61 aaatagccat ctataaacaa taaaaatata tagaagatat catttgttaa ttaacaagca
      121 tttacgtctc tttttttcgg atttaattgt gaaattttaa tcatcatatt cacaaaatca
      181 attatctgtg aaatttttcc cagacatggt ttctttcata tttgacattt attaataatc
      241 atcattagaa tccacgaaaa ataatatatt catacaaaca cagacatctt ttgaaacgaa
      301 tgatacattt ttatgatgaa tgcttaaaac aatatttaat cgacacacat cactgtcatt
      361 aatgtaaagc aacatagctc accaataata caatggacaa ctctaccggc attgagcgct
      421 ggtcggagga aataaggatt tttacatgaa cttgaagaag atttttttca gtgctgtcac
      481 tgtttcagtt ctctgcgcat taacgggatg tgactacatt gaagaaggca agccagaaag
      541 tagtttactg aaacaacaag aagaacataa taataaaatt gtgctacttg aaaaacaaca
      601 agcacagcta aagagtcaac tcgaaactat tcaaaaacaa caaactggca taataaacag
      661 tacaaagact ttaactcacg taattaaatc cgttaaagac caacaaaata cttttatttt
      721 taccgagttt aacccagcaa aaacgaagta tttcatcctg aacaacggtt cggtggcttt
      781 agcgggtcga gtgttatcca ttgacgccac agagaatggt agtgttattc atatctcact
      841 ggtcaactta ttaagtacac ctatctcaaa cattggtttt aatgcgacat ggggtggtga
      901 aaaacctgtc gatgccaaag agtttgcccg gtggcaacaa ttacttttca acacatcaat
      961 gaaatccaca ttgaaattat taccaggtca atggcaagac attaatttga ccctgaaggg
     1021 tgtatcgccc aataacctgg gatatctgaa gttagccatc aacatggaaa atattcagtt
     1081 cgacaatctt ccctctgctg aaaatcggca gaaaagaagc aaaaaataaa tgcagttagt
     1141 acgcttataa caaggaatag cgagcatcat taatgaacaa aatatttaaa gttatctgga
     1201 accctgcgac agggaattat actgttacca gcgaaacggc aaaaagccgt ggcaagaaat
     1261 ctgggcgcag taagctgtta atttctgcgc tggttgcggg tggaatgttg tcgtcgtttg
     1321 gggcattggc gaatgccggg aatgacaacg gtcagggtgt tgattacggt agtggatcag
     1381 ctggcgacgg ctgggttgct ataggcaaag gggcgaaagc aaatactttt atgaacacca
     1441 gtggttccag tactgctgtg ggttatgacg ctatagctga aggccaatat agctctgcca
     1501 tcgggtcaaa aacccatgcg attggtggtg catcaatggc ctttggggtt agtgcaatat
     1561 cagaaggcga tagaagtata gcactgggtg cctcttcgta ttcattgggc caatactcaa
     1621 tggccctcgg ccgttattca aaagcattgg gtaaattgtc tattgctatg ggggactctt
     1681 ccaaagcgga aggagcaaac gccattgccc tggggcgtag cagtgtagct agcggtactg
     1741 acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt gcgataggtg
     1801 ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag gcaaaaggga
     1861 ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact atcgcattag
     1921 gaaatggcag ccaggctctg gcagataatg caatcgccat aggccagggc aacaaagctg
     1981 atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta aacaccattg
     2041 ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt agtaatagca
     2101 gtgccaacgg tattaactct gtcgcgctgg gcgcagattc cattgcggat ttagacaata
     2161 ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa aatggcgcga
     2221 tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt agcgactcgg
     2281 tagcaaaaaa ggcttggagg aggggctgca gtagatgttg atgacggtac tgttacagca
     2341 ccaacctaca atttaaaaaa tggtagcaaa aataacgtag gggctgcgct cgctgtactt
     2401 gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat acagcgctgc tcatggtact
     2461 agtagcccaa ctgccagcgt aatcaccgat gttgcggatg gcacgatttc agcctccagt
     2521 aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg acgatgtcga agccaacacc
     2581 accaatatca ccaacctgac ggattccgtt ggtgaccttc aggctgatgc cctgctctgg
     2641 aacgaaacta aaaaggcatt cagtgcagct cacggccagg ataccaccag caaaatcacc
     2701 aacgttaaag atgccgacct gacggctgac agcactgatg ctgttaacgg ctctcagctg
     2761 aaaaccacca acgatgctgt ggcgacgaat accaccaata tcgccaataa cacttccaat
     2821 attgccacta acaccaccaa catctctaac ctgactgaga cggtgactaa tcttggtgag
     2881 gatgcgctga aatgggataa ggacaatggt gtattcacgg cagctcatgg caccgagacc
     2941 accagcaaaa taaccaacgt taaagatggc gacctgacga ctggcagcac cgatgccgtt
     3001 aacggctctc agctgaaaac caccaacgat gccgtggcga cgaataccac caatatcgcc
     3061 actaacacca ccaacatctc taatctgact gagacggtga ctaatcttgg tgaggatgcg
     3121 ctgaaatggg ataaggacaa tggtgtcttc actgcagctc atggcaacaa taccgccagc
     3181 aaaatcacca atatcctgga cggcacagtc actgcaacca gttccgatgc cattaacggt
     3241 agccagcttt atgacttaag cagcaatatc gccacctact tcggcggcaa tgcttctgtg
     3301 aatactgacg gtgtgtttac cggtccaacc tacaaaatcg gtgaaacaaa ttattataac
     3361 gtcggcgatg cactggctgc gattaactcc tcatttagca cgtctctcgg cgatgctctg
     3421 ctttgggatg ccaccgcagg taaattcagt gccaaacacg gtactaatgg tgacgcaagc
     3481 gtgatcactg atgtcgcaga tggtgaaatt tcagactcca gttctgacgc agtaaacggc
     3541 tcacaactcc acggcgtgag cagttatgtt gttgatgcgc tggggggtgg tgccgaagtc
     3601 aatgcagacg gcaccatcac tgcgccgacg tacaccattg ctaatgctga ttacgataat
     3661 gtcggtgatg ccctgaatgc tatcgatacc actcctgacg acgctctgct ctgggatgcg
     3721 gacgccggtg aaaatggtgc atttagcgcc gctcacggaa aagataaaac tgccagtgta
     3781 atcactaacg tcgctaacgg tgcaatctct gctgccagca gcgacgcgat taacggctca
     3841 caactctata ccaccaataa gtacatcgct gatgcgctgg gtggtgacgc agaagtcaac
     3901 gctgacggca ccatcaccgc accgacttac accattgcga acgccgagta caacaacgtc
     3961 ggtgacgccc tggatgcgct tgatgataac gccctgctgt gggataagac tgccaatggc
     4021 ggtgctggag cctacaatgc cagccatgac ggtaaagcca gcatcatcac taatgtcgct
     4081 aatggcagta ttagtgagga cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg
     4141 aatatgatga ttgagcagaa cacccaaatt atcaatcagc tcgctggtaa caccgacgca
     4201 acctatatcg aagaaaatgg cgctggtatc aactacgttc gtactaacga caacgattta
     4261 gcctttaacg atgcaagcgc ctctggtgtc ggcgctacag ctgtaggtta taacgctgtc
     4321 gcgtctggtg ccagcagcgt agccattggt cagaacagca gcagcaccgt tgataccggt
     4381 attgcgctgg gtagcagctc cgtttccagc cgtgtgattg ccaaaggttc tcgtgacact
     4441 agcgtaacgg aaaatggcgt ggtgattggt tatgacacca ctgacggcga actgctaggt
     4501 gcattgtcaa ttggtgatga cggtaaatac cgccaaatca tcaacgtagc tgatggttca
     4561 gaagcccatg acgccgttac ggttcgccag ttgcagaacg ctattggagc ggtcgccact
     4621 acgccaacca agtactttca cgccaactca acggcagaag actcactggc cgttggtgaa
     4681 gactcgctgg caatgggtgc gaaaactgtc gttaatggta atgcaggtat tggtatcggt
     4741 ttgaacactc tggttctggc tgatgcgatc aacggcattg ctatcggcag taacgcacgc
     4801 gcaaaccatg caaacagtat cgcaatgggt aacggttctc agaccacccg tggtgcacag
     4861 actggctaca ccgcctacaa catggacgca ccgcagaact ctgtaggtga gttttctgtc
     4921 ggtagtgaag acggtcaacg tcagatcaca aacgtcgcag ctggttcagc ggataccgat
     4981 gcggttaacg tgggtcagtt gaaagtcact gatgagcgcg tagcgcaaaa tacccagagc
     5041 attactaacc tgaacaatca ggtcactaat ctggatactc gcgttactaa tatcgaaaac
     5101 ggtattggcg acattgtcac caccggtagc accaagtact tcaagaccaa caccgatggc
     5161 gtagatgcca acgcccaggg taaagatagc gttgctattg gttctggttc cattgctgcc
     5221 gctgacaaca gcgtcgcact gggtaccggt tccgttgcag aggaagaaaa tacaatctct
     5281 gtaggttctt ctactaacca acgccgtatc accaacgtag ctgccagtgt taatgccacc
     5341 gatgcggtta acgtgtcgca gctgaaatct tctgaagcag gcggagtgcg ctacgacacc
     5401 aaagctgatg gttctatcga ctatagcaat atcaccctcg gtggtggcaa cggcagtacg
     5461 actcgtatca gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa ctacgcgcag
     5521 ttgaagcaaa gcgcgcagga aacgaagcaa tacaccgatc agcggatggt tgagatggat
     5581 aacaaactgt ctaaaactga aagcaagttg agcggtggta tcgcttctgc aatggcaatg
     5641 accggtctgc cgcaggctta tacaccgggt gccagcatgg cttctattgg tggcggtact
     5701 tacaacggtg aatcggcagt tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg
     5761 gtctacaaat tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc
     5821 ggtattcagt ggtaatgatc cattaagtta gtgtgactaa gggcaaacgc caaattgcct
     5881 gatgcgttac gcgcatcagg caagataagg cgctccgggt caacaatcta agggcaattc
     5941 tctgatgagg attgcccttt tctttaccag acatctcccc ccacaagaat tggccctacc
     6001 aattcttcgc ttatctgacc tctggttcac aattccccaa ttaaaactca catcaatatt
     6061 gccaatacat aacatttagt taaccattca ttgtcattat ccctacacaa cacaattggc
     6121 agtgccactt ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg
     6181 cgtggactct aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa
     6241 tatctggctt tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat
     6301 taagctcaaa ctgaaaggat acgtcgccgc ctcatggacg gtagcaatcg cccttgccgt
     6361 ggctttgctg ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggctt
     6421 cttctatggt ttgtggccca tcgcgtggat cattattgca gcagtgttcg tctataagat
     6481 ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct attctttcga taacccctga
     6541 ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc gctttccttg aaggagccgc
     6601 aggctttggc gcaccggtag caattaccgc cgcattgctg gtcggcctgg gttttaaacc
     6661 gctgtacgcc gccggactgt gcctgattgt taacaccgcg ccagtggcat ttggcgcgat
     6721 gggcattcca atcctggttg ccggacaagt aacaggcatc gacagctttg agattggcca
     6781 gatggtgggg cgtcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat
     6841 tatggacggc tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg caggcggctc
     6901 gtttgccatc gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat
     6961 ctcttcgctg gtatcactgc tctgcctgac gctgttcctc aaacgctggc aaccagttcg
     7021 cgtattccgc ttcggtgact tgggggcgtc acaggttgat atgacgctgg cccacaccgg
     7081 ttacaccgcg ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga ccgctaccgt
     7141 aacactgtgg agcatcccgc cgtttaaagc cctgttcgca tcgggtggtg cgctgtatga
     7201 gtgggtgatc aacattccgg tgccgtacct caataaactg gttgcccgta tgccaccagt
     7261 ggtcagcgag gctacagcct atgccgccgt gtttaagttt gactggttct ctgccaccgg
     7321 caccgccatt ctgtttgctg cactgctctc gattgtctgg ctgaagatga aaccgtctga
     7381 cgctatcagc accttcggca gcacgctgaa agaactggct ctacccatct actccatcgg
     7441 catggtgctg gcgttcgcct ttatctcgaa ctattccgga ctgtcatcaa cactggcact
     7501 ggcactggcg cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg
     7561 ggtattcctg accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac
     7621 cgcagcacaa caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg
     7681 cgtcaccggt aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct
     7741 ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg
     7801 tatggtgggc gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg
     7861 attgttttac ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat
     7921 gaaaaaaacc tggaagcggg catgaagttg cccgctgagc gccaactggc gatgcaactc
     7981 ggcgtatcac gtaattcact acgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg
     8041 ctcagtcgac gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa
     8101 aacatcgtcc agccgctaaa aacgctgatg gccgatgatc cggattacag tttcgatatt
     8161 ctggaagccc gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca
     8221 cctggcgaca aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat
     8281 atcgcctcac aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc
     8341 gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc
     8401 cgtcagcgga tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct
     8461 gtcattgacg ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac
     8521 cttagttttg ttcacaccac catgaaacga ttcgatgaag atcaggctcg ccacgcacgg
     8581 attacccgcc tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt
     8641 ccgcagccag cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact
     8701 atatggatgg tggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag
     8761 aagtggcact gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc
     8821 tgtttaatga gaaattgtcg atgccggtag cactggcacc ggtgggtttg tgtggcatgt
     8881 atgcgcgtcg tggcgaagtt caggcagcca aagcggcgga cgcgcatggc attccgttta
     8941 ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa
     9001 tgtggttcca gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag
     9061 caaaagcagc gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggcgcac
     9121 gctaccgtga tgcgcattca ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc
     9181 aggcggtgac gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt
     9241 taggtaatat ctcagcttat ctcggcaaac cgaccggact ggaagattac atcggctggc
     9301 tggggaataa cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgatttct
     9361 gggatggccc gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac
     9421 gttttggtgc tgatggaatt gtggtttcta accacggtgg ccgccagctg gacggtgtac
     9481 tctcttccgc tcgtgcgttg cccgctattg cagatgcggt gaaaggtgat atcgccattc
     9541 tggcggatag cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg
     9601 acaccgtact gctgggtcgt gctttcctgt atgcgctggc aacagcgggc caggcgggtg
     9661 tagctaacct gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg
     9721 cgaaatcgat cagtgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagttgc
     9781 ctacggcact ggctccgatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcacc
     9841 ctaaccctct ccctcaggga gaggggaccg ttcggcgctg tatgtactcc ctcactctga
     9901 aacgacaccg cactcttttt ttctccctcg cccctccggg gagggccggg gtgaggggaa
     9961 aaggccgcac ggttcaccaa ttttctgcta tcctgccccc gcactaaggg ggcagtatgc
    10021 taaacatcgt actttacgaa ccagaaattc cgccaaatac tggcaacatc atccgtcttt
    10081 gcgctaatac cggctttcgt ctacatatca tcgaaccgat gggatttgcc tgggacgaca
    10141 agcgcctgcg ccgcgcgggg ctggactatc acgagtttac cgccgttacg cgtcatcatg
    10201 actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct gtttgccctc accacgaaag
    10261 gtacgcctgc tcacagcgcc gtaagctatc aggatggcga ttatctgatg ttcggcccgg
    10321 aaacacgcgg cctgccagcg agcattcttg atgccctgcc cgctgaacaa aaaattcgca
    10381 ttccgatggt gccggacagc cgcagcatga atctgtccaa tgcggtgtcg gtagtggtgt
    10441 atgaagcctg gcggcagctg ggatatccgg gagcgttgtt gagagattag ttactgtatg
    10501 ccggatagat gattacatcg catccggcac gatcacagga cattagatcc catccccata
    10561 ctcaaatgta tggttaatac cgttgaaatg ctggtccata tccattgatg gcttatcgct
    10621 gtctggttta ccgacaatac gagccggaac gccagcggcg gttgtatgcg gcggcaccgg
    10681 ttgcagcacc acggaacctg cgccaatctt cgcgccgcga ccaacttcaa tattgccgag
    10741 gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt ttcgggtgac ggtcaccacc
    10801 agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc gatacgtcgt tttcaattac
    10861 cgccgtttca cccacaacga tgcccgtggc gtggtcgagc ataatgccac ggccaatttt
    10921 tgctgccggg tgaatatcga cctggaacgt cacagaaacc tggttttgca gaaagattgc
    10981 caatgcgcga cgcccctgat tccacaacca gtgaccgatg cgataggcct gcaaggcatg
    11041 aaaacccttc aggtataaca acggggttga gtatttatcg actgccgggt cgcgggtacg
    11101 caccgcctga atatcacagg ccgcagaggc gatcatttcc gggtcagcgg cgtaggcttc
    11161 ttcaaccact tcacggatag caatagcagg cataattggc gatgacagct tgttcgccag
    11221 catgtagctc agtgcactgc caaggttttc gtgcttgagt agcgtcgcgt ggtaaaaact
    11281 ggccagcatt ggctcacagt ccgccagcgt tctggcttcg gctttaatat tgttccagac
    11341 aatttccagt tcttcacacg acattgctta ctccacacga tgagataatg accggcccgt
    11401 tctgcgcggg ccgggtcata gcggtaacaa aggttccctg gggttagtga ctgctgcgct
    11461 cgtccttgcg tgcacgacct aataatgtca atgctgcctc gcgcgcgttt tttccgcaat
    11521 ataatacttg ataaatttcc tcggttattg gcatttcaac gccgaagcga tgcgccagtt
    11581 cgcggacttc tttcgtattg cggtagcctt ccaccacctg accaatcttc tcctgcgcgc
    11641 tttgtacatc catgccctga ccgagcatca taccaaaacg gcggttacgc gactggttgt
    11701 cggtacaggt aagcaccaga tcgcccagcc ccgccatgcc cataaaggtg gcaggatcgg
    11761 cacccagcgc cgcaccaaga cgcgacattt cagccagccc acgggtgatc agcgccgtac
    11821 gcgcattcgc accaaaaccg ataccgtcag acatccccgc gccaatggca atgacgtttt
    11881 tcaccgcgcc gccaagctgc acgccaatga aatccgggtt gctgtaaacg cggaaacttt
    11941 tgccgcagtg cagcagctgc tggagatcat cggcaaaggt ctgatcggtc gaggccagcg
    12001 aaatagctgt cggtaaacct gccgccagtt ctttcgcaaa cgttgggcca gagataaccg
    12061 ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc ctgtaacaga cgtccggttt
    12121 ccgcttccag ccctttggtc gcccacacca gacgcgcatc aggacgcatc agcggtttaa
    12181 tctggcgcag cacttcacca aagacatggc tgggtacgac gacgagaata ttacgactgg
    12241 ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt atcgggaaaa ggcacatcgg
    12301 ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc gatatgttca gggtcatggc
    12361 cccagaggac aacctcgtgg ccatttcttg ccagggtaat ggcaagagcg gtgccgtacg
    12421 agccggcacc gatcacagtc attgaagcat tacgttggtt catcaggcat cctgatgttc
    12481 ttcagtacct tcgccagcct gctgctgcaa atagttcatg aacagcgcat cgaagttaac
    12541 cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc atgctggtga tgcactcacg
    12601 agcatacggg aacagaatgt tcgggcagta tgcgcccagg caatgtgcca tctgggtgcc
    12661 ttcgataccc gcgatggaga aaataccgcc ctgctgaact tcacacagga acgcggtttc
    12721 ttcgcccaag gaggccgtta cggtaacacg cagtaccact tcgtatacgt catctgccag
    12781 ttgggaagat gccgtatcca gatcaagttt aacttctggt tgccaatctt tctggaaaac
    12841 gtgcggcgcg ttcggcgctt cgaaagagat atccttggta taaatacgtt ggatctggaa
    12901 agtcatttca gtgttgtttt gttctgacat gtgtagaaaa cccttaagtg ttgtccttaa
    12961 atactgcgca atgtaatacc aacgttcaca cgttatttca gcaggggatc cagtccacca
    13021 cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt gctgtgcgtc aataaaaatc
    13081 tggggaaccg tggtgcaacc gctgcgtttg atcatctctt cacgcttggc ggcattgcca
    13141 tcgatcggca actcctggaa actcacgccc ttgctgctca gcagtgcttt tgcacgatgg
    13201 caatacgggc aggtttcttt ggtatagatt tcaacattgg ccataattta gctcctgttt
    13261 ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat ggagtgtccc cagaagctta
    13321 taacaataag tcgctggggg cctaattgca gctaacggcc tgcatcatga aagacgacag
    13381 gtaaattatt tgccgcgcac cagaggcaag ttttcgccag cccagccagc aacgccctct
    13441 ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg cgtttgcagg ctcctggcac
    13501 tgcatgccag aaccgtctac cacgataacc gatttgtctt tgtgcttctc aagctcacca
    13561 acattgttgg ctttgatttc gctcggcaac agattgatag aacctgcgat atggccttta
    13621 cggaagtcat cacgctgacg taaatccaca accacagcgt cttctttgtt gatcagacgc
    13681 gtagcttcac cacgagtaat caccttcact ttcgaggtca ggctcttaaa cgtagtcaca
    13741 agaaccgcca ccagtaacgc gatccaggcg atactcagta tgggatgacg gccaacaaat
    13801 tgcataattt cttgcatggg gggtaacaac tcccgacgta gtgattaaaa aaaccaggaa
    13861 aggagtatac ctgcgcggtg cggcaaatac agccagcgcg ttaactggaa tgcaattttg
    13921 cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct tgccgcgagc ggtcgtatat
    13981 tttgatcttc agaggctatt ttatcgattc agctgtagta aaattacgca aattttgact
    14041 cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac ctatggtact ggtgattctg
    14101 gatggctatg gctatcgtga agaacagcag gataacgcca tttttagtgc taaaaccccg
    14161 gtaatggatg cactgtgggc caatcgtccg cataccctga tcgacgcttc cggtctggaa
    14221 gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag gccacgttaa cctgggtgcc
    14281 ggccgcatcg tgtatcagga cctgactcgt ctggacgttg aaatcaaaga tcgcgctttc
    14341 tttgctaatc cagtgctggc tggcgcggta cataaagcga aaaacgcagg taaagcagta
    14401 cacattatgg gtctgctctc cgcaggcggc gtacacagcc acgaagatca catcatggcg
    14461 atggtagaac tggcagctga acgcggcgca gaaaaaatct acctgcacgc attccttgac
    14521 ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga aaaaatttga agagaaattt
    14581 gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc gttactacgc gatggaccgc
    14641 gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc tgactctggc gcagggtgag
    14701 ttccaggccg atactgccgt tgctggtttg caggcagctt atgctcgcga cgaaaacgac
    14761 gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc cagatgcggc aatggaagac
    14821 ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg cgcgtgaaat cactcgtgca
    14881 ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag tggttaacgt cgatttcgtg
    14941 atgctgaccg aatacgccgc tgacatcaaa actgcggttg cttacccacc cgcttccctg
    15001 gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa ctcagttgcg tatttccgaa
    15061 accgaaaaat atgcccacgt tactttcttc ttcaacggtg gcgtagaaga gtcgttcaaa
    15121 ggcgaagatc gcattctgat caactcaccg aaagtggcta cctacgatct gcaaccggaa
    15181 atgagctccg cagagctgac cgaaaaactg gttgcggcca tcaagagcgg caaatacgac
    15241 accattatct gtaactatcc gaacggcgac atggtaggtc acaccggggt gatggaagcg
    15301 gcggttaaag cggttgaagc actggatcac tgcgtggaag aagtcgcgaa agcggttgag
    15361 tccgtgggtg gacaactgct gatcaccgct gaccacggta acgctgaaca gatgcgcgat
    15421 ccggcaacgg gtcaggcaca cacggcacac accaacctgc cagttccgct gatttacgtt
    15481 ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc gccgaccatg
    15541 ttgtcgctga tgggtatgga aatcccgcaa gagatgactg gtaagccgct gttcatcgtg
    15601 gaataatccc tccccatgag gggaaaggcg attaatacca tgacacgggc cgtgaaaccg
    15661 cgcaggtttg caatcaggcc catcatctac gccagcgttc ttagcgctgg cgtattgttg
    15721 tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca aatctattca ggccgatatc
    15781 gccgcaaaag agcgcgcggt acgccaaaag caacaacaac gcgcaagcct gctcgcacaa
    15841 ttgaaaaagc aggaagaagc gatctctgaa gccacccgta agctgcgcga aacgcaaaac
    15901 acgctcaatc aactcaataa acagattgat gagatgaacg cgtcgattgc caaactggag
    15961 cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac tggatgccgc attccgtcag
    16021 ggcgagcata ccggtattca gctaattctc agcggtgaag aaagccagcg tggacagcgt
    16081 ttacaggctt atttcggcta tctcaaccag gcgcgacaag aaaccattgc ccagttgaag
    16141 caaacgcgtg aagaagtcac catgcagcat gctgaactgg aagagaaaca aagcgagcaa
    16201 caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc tctgaacgag
    16261 cgtaaaaaga cgctggcggg gctggaatct tccatccagc aaggtcagca gcagctaagt
    16321 gaactgcgtg ccaacgaatc ccgtctgcgt aacagcattg cccgtgctga agccgcggcg
    16381 aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg ttcgcgagcg ccagaaagaa
    16441 gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct gatgtcccga
    16501 actggtggcc tgggtgcgcc gcgtggtcag gcattctggc cggttcgcgg gccgacgctg
    16561 catcgctatg gcgagcagct acagggtgaa ttacgctgga aagggatggt tatcggtgcc
    16621 tctgaaggta ctgaagttaa agcaattgcc gacggtcgag tgattctggc tgactggctg
    16681 caaggttacg gtctggtggt ggtggttgag cacggtaaag gcgacatgag tctttacggc
    16741 tataatcaga gcgcactggt gagcgttggt tcgcaggttc gcgcgggcca gccaattgca
    16801 ctggtgggca gcagtggcgg tcagggtcgg ccttcactct atttcgaaat tcgccgccag
    16861 ggtcaggcgg tcaatccaca gccgtggttg ggaagataag ttttgtttcc atttcgtcgt
    16921 aacgttcttg catttgccgc tctgttggcg ctctcctccc ccgtacttgc tggcaaactt
    16981 gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg aaaaccaggt gctggcgatg
    17041 ccttccgcta tctccgtcgc tgtattaccc gattcaccgc acgccagaga gatggcgacc
    17101 aaagcgcata acagcggtca cgaagtgttg attcatctcc cgatggcacc gttgagtaaa
    17161 cagccgctgg agaaaaatac gctacgcccg gagatgagca gcgacgaaat tgagcgcatt
    17221 attcgtagtg cggtcaataa cgtgccctat gccgtgggga tcaacaacca catgggcagc
    17281 aagatgacct ctaacctgtt tggtatgcag aaagtgatgc aggcgctgga gcgttacaat
    17341 ctttacttcc tcgacagcgt aaccatcggt aatacccagg cgatgcgcgc cgcgcaaggt
    17401 actggcgtga aggtgatcaa acggaaggtg ttcctcgacg attcgcagaa cgaagcggac
    17461 atccgtgtgc aatttaatcg cgcaattgat ctggcgcgtc gcaacggttc aaccattgcc
    17521 attgggcatc ctcacccttc aacggtacgc gtgctacaac agatggttta taacctgccg
    17581 ccagacatta cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt tgatacttct
    17641 acaccgccga aaaacgctgt gcctgatgca ccgcgtaatc cgttccgtgg cgtgaagctg
    17701 tgcaaaccga agaaaccgat agaacctgtt tatgctaatc gcttctttga agtattaagc
    17761 gaaagcatca gccagagcac gctgatcgtt tacttccagc atcagtggca aggctggggc
    17821 aaacagcccg aagcggcgaa gtttaacgct agcgcaaatt aaaggcgcta cgcgtaatgc
    17881 gatggtgcga ccgcgttttg tcgcgccatt gccataagcg gaaagaccac agtaacgcct
    17941 ggtaaccgac cttcacgctg cgcacattgg taatcaggcg cttatacata cccggaagta
    18001 aatatctcgg caatcatccg ttggcgggta agaatatccg gctctttgcg
//
LOCUS       DAJTRD010000080        17636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTRD010000080 DAJTRD010000000
VERSION     DAJTRD010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.080"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(1..673)
                     /gene="chbR"
                     /locus_tag="OO315_002957"
                     /pseudo
     CDS             complement(1..673)
                     /gene="chbR"
                     /locus_tag="OO315_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
     gene            complement(681..1031)
                     /gene="chbA"
                     /locus_tag="OO315_002958"
     CDS             complement(681..1031)
                     /gene="chbA"
                     /locus_tag="OO315_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5891790.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(1090..2438)
                     /gene="chbC"
                     /locus_tag="OO315_002959"
                     /pseudo
     CDS             complement(1090..2438)
                     /gene="chbC"
                     /locus_tag="OO315_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2523..2843)
                     /gene="chbB"
                     /locus_tag="OO315_002960"
     CDS             complement(2523..2843)
                     /gene="chbB"
                     /locus_tag="OO315_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5891791.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(3142..3480)
                     /gene="osmE"
                     /locus_tag="OO315_002961"
     CDS             complement(3142..3480)
                     /gene="osmE"
                     /locus_tag="OO315_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5891792.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3682..4509
                     /gene="nadE"
                     /locus_tag="OO315_002962"
     CDS             3682..4509
                     /gene="nadE"
                     /locus_tag="OO315_002962"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5891793.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4739..5626
                     /gene="cho"
                     /locus_tag="OO315_002963"
     CDS             4739..5626
                     /gene="cho"
                     /locus_tag="OO315_002963"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5891794.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5586..6161)
                     /gene="ves"
                     /locus_tag="OO315_002964"
     CDS             complement(5586..6161)
                     /gene="ves"
                     /locus_tag="OO315_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5891795.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(6364..6849)
                     /gene="spy"
                     /locus_tag="OO315_002965"
     CDS             complement(6364..6849)
                     /gene="spy"
                     /locus_tag="OO315_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5891796.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(7179..8147)
                     /gene="astE"
                     /locus_tag="OO315_002966"
     CDS             complement(7179..8147)
                     /gene="astE"
                     /locus_tag="OO315_002966"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5891797.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(8140..9473)
                     /gene="astB"
                     /locus_tag="OO315_002967"
                     /pseudo
     CDS             complement(8140..9473)
                     /gene="astB"
                     /locus_tag="OO315_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9470..10948)
                     /gene="astD"
                     /locus_tag="OO315_002968"
     CDS             complement(9470..10948)
                     /gene="astD"
                     /locus_tag="OO315_002968"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5891798.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10945..11979)
                     /gene="astA"
                     /locus_tag="OO315_002969"
     CDS             complement(10945..11979)
                     /gene="astA"
                     /locus_tag="OO315_002969"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5891799.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11976..13196)
                     /gene="astC"
                     /locus_tag="OO315_002970"
                     /pseudo
     CDS             complement(11976..13196)
                     /gene="astC"
                     /locus_tag="OO315_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13642..14448
                     /gene="xthA"
                     /locus_tag="OO315_002971"
     CDS             13642..14448
                     /gene="xthA"
                     /locus_tag="OO315_002971"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5891800.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14615..15325
                     /locus_tag="OO315_002972"
     CDS             14615..15325
                     /locus_tag="OO315_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5891801.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            15330..16007
                     /gene="ydjY"
                     /locus_tag="OO315_002973"
     CDS             15330..16007
                     /gene="ydjY"
                     /locus_tag="OO315_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5891802.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            16022..16729
                     /locus_tag="OO315_002974"
     CDS             16022..16729
                     /locus_tag="OO315_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5891803.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16729..17277
                     /locus_tag="OO315_002975"
     CDS             16729..17277
                     /locus_tag="OO315_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5891804.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            17287..17613
                     /locus_tag="OO315_002976"
                     /pseudo
     CDS             17287..17613
                     /locus_tag="OO315_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         4364 a         4684 c         4304 g         4284 t
ORIGIN      
        1 tctcattaat aatctgcatt ggtgttttgc cataatatcg ttgagtcgct cgcgtcaaat
       61 attcctgtga tttggctgac aacgtcacca tattctccag cgccgattca ctaaactgct
      121 ctttatcatg catcttttct accgtacttt tcagccacta cggtacatca tcaatcacct
      181 gttcttcgcg gtaatgacgt aaacggttaa tgacataaaa agtaaccatc tcaacaaact
      241 cttccagccc tgtttcgcgg aaatttaatg aagaaatcac tgtttccaca taggtgagaa
      301 aagcgttatt ggtccggtat acctgcgaag caacaaagca ataaggcaac aacggcaggt
      361 aatgctgctc aaaaaagcgt ttactgatcc caacgttcaa tatgcgcgtg gcaccaaact
      421 cataaaaact ttgatggtgc gaacctaacg gaataaaaac aaaatcgccc cgttccagta
      481 acacgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga gtaaattcat
      541 aatagtcgtg ctgatgcagt ccgctgatac tctcagtttt gttatagata aacacatgga
      601 agtttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc gcgttaatca
      661 ctggctgcat catcgactcc ttatgccttc agtttttcat gaagctcaat taattcagta
      721 atcagttcac gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac cagcaccaga
      781 ctcactttca tcttaccttc gcccgcatcg ccttcaatca gtttcgtctg taccagatgc
      841 gcttcattca atgccatccg tgactgatcc atcatggctt tcgctgcggc aaaatcgccc
      901 tgcttcgcct gtttcaatgc ggcatacgcc aggctacgcg cttgtccaga gttgatgata
      961 agccccatca ccacttcttc cagctcttca gcttctgttt gcgtatcagg aatattatcg
     1021 agatccatca tacatcgttc ctctctcttc ttaccggcaa gattacccgt accggcatcg
     1081 attaaaattt caggggttag cgatatcttc ttcgctctct tctttatcaa tcgcattttg
     1141 tgctttgtta gccaccacaa caaagggcag ataaattaac gttgcgatgc caaggttgaa
     1201 gagtgcgacc agcaatgcgg cgacgctacc gttggtgtta aagaaggctc ccagaccggt
     1261 tggcatggtc cacggtgcaa tattggtcac cggaggaatg atgcccatgt agtacgctgc
     1321 gagggtgatt gccgccagaa tcggttgtac cagtacaaac gggataaaca tcaccgggtt
     1381 catgataatt ggcagaccaa acagaatcgg ttcattaatc tggaagatgc cggacggcag
     1441 cgccagtttt gccacctgac gataatcagc acggcgagag gcgataaaga tagccaggat
     1501 caggcctaaa gtcgcaccac tgcccccaag gaaaataaag gagtccagca tcggcttggc
     1561 ccagatatgg aaggtcttac cggctgccag cgccgcttcg acggaaccat attgctgata
     1621 ggtcgcgata ttttccagcg cccacggcgt cataatgccg ttgtccagtg cggtcagcgc
     1681 cagcgcgcca tgaataccga agaaccagag cagtggaaca aagatcacat aggcccagcc
     1741 caccacgcta cccaacgatg ccagtggggt tgagatggta tccataatga tctgatggaa
     1801 gttggtgccc caggtattca acgcccaggc aataatcccc atcacggaaa gaataataaa
     1861 gccgggaatt aatgccgaga aagaacgcga tactgaagca ggtacgctgt cgggcagttt
     1921 aatgacccaa ttgcggcgga caataaaggt aaacatttct gccaccacca ggccaataat
     1981 aatcccggag atgatattcg ccccacctaa ccagtttgcg ccaaccgcat aggcctcacc
     2041 gacactatat ggggtgacgg tcataaatgc tgcaacggat aacaacccag ccgccagcgc
     2101 atcgacttta cgctcttctg ccagcgccat gccaataaag aacggtgcca ttaaagacat
     2161 tattcctaat gttccgttat atacgttgcc gccaatacct ttcagaccat taagtgtttc
     2221 aatggttgag gcgtcgaggc gaatacctaa ggaataaaaa acgacccctc cccaaagctt
     2281 agaaaaacgt tgttaattaa tacaaacatg gcccccgcaa gggttaacgg cattaagcga
     2341 ataaagccat ttttgattgc attaacgtgt ggctgctttc ctattttaac tgcaaaaggg
     2401 aggagtacct tttcaagcga tgcaataaca ttactcatag aaaaataccc ttaaaaaccg
     2461 caatttaaat attacggtat tgatttatga aataactctt tgacgggaaa atttaaaata
     2521 aattaatttg ctgcggcttt tttaatcgct gcaaccgcag ccttaagcac gcctaaacca
     2581 tcgactttgc cataaagcag cgagtcaatt acttcaaccg gtttgttggg taacaaacgc
     2641 tggatttcgg gcaacatata agcaatctgc ggccctaata acacgacatc ggcattctga
     2701 cctttttcac cagccagtgt ttccggaaat gcttcaataa tgaccggaac ttcatatttt
     2761 tctgcctgtg cgcgcatttt tgataccagt aaagagatag acatgcccgc agaacaaaac
     2821 agataaatgt gtttcttttc cataaaactg ccctcatcga cgattatctg tcagccagac
     2881 actccgcaag ccttgacctg cttccatgct ctgggtaact tgcgaaacca acatgacttt
     2941 ttttgtggct gaaaggagta tacggtatag acctgagcaa tagtatttct ttgaccttat
     3001 aaattgtttc tccttgacct gtggttatga ccctcttcac atttcgggca aataattcgc
     3061 gaagataatt aagaactcag gccaaaaaaa accggcgcaa tggccggttt ccgttgttac
     3121 tcaagctttc agacgaattg attacttcgc agcctgtgga tcagtgtcgt attcagcaca
     3181 ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc
     3241 tgctttacca tcacgttgac ccaggatgta ggtctggcaa gtaccgcgag catggatcat
     3301 gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt
     3361 tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat aagctgtaca
     3421 acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat tcttgttcat
     3481 attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg gcgctttttc
     3541 aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt ttaaccacca
     3601 ttcagcccgc tgtcgcttgt cgtttcagta gcaacgggtt agctttaagg aagttttgtc
     3661 ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc gctgggcgca
     3721 aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg tcgattttct gaaaagctac
     3781 ctgcaaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg tcaggactcc
     3841 acgcttgccg gaaagctgtg ccagatggcg attaatgagc tgcgccagga aactcgcaac
     3901 gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggctga cgaacaagat
     3961 tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa tatcaagggc
     4021 gcggtattgg ctagcgagca ggcattgcgg gaagcaggca ttgaactgag cgattttgtc
     4081 cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc gggtatgacc
     4141 agcggtgtcg tggtgggcac cgatcatgca gcagaagcca ttaccggatt cttcactaaa
     4201 tatggtgacg gcggtacgga tattaatccg ctgtatcgtc tcaacaaacg ccagggtaaa
     4261 cagttactga cggcattagg ttgcccggaa cacctttata agaaagcccc aactgccgat
     4321 cttgaagatg atcgcccttc tctgccagat gaagtggcac tcggcgtgac ctatgacaat
     4381 atcgacgact atctggaagg gaaaaaccta cctgaacagg tcgccagaac aatagagaac
     4441 tggtatctga aaaccgaaca taaacgccgt ccgccaatta acgttttcga tgatttctgg
     4501 aaaaagtaat aatttgcacc tattggattg tgcgaaaaag agtaatttgt tcacgccgga
     4561 tgcggcgtga acgccttatt ctacctacaa aactatgcaa attcaataca ttgcaggagt
     4621 cgaataggcc tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc aacccgcctc
     4681 ttttttaatc gcctcccgcc tgttacactg gatagataac cagcattcgg agtcaacagt
     4741 ggtacggcgt ttaacttctc cgcggctcga atttgaagct gcggcaattt atgaatatcc
     4801 cgaacattta cattcattcc ttaatgactt acccacccga ccaggggtgt atctgtttca
     4861 tggcgaaagt gacaccatgc cgctctatat cggcaaaagc gttaatatcc gcagtcgggt
     4921 gctttctcat ttacgcaccc cggatgaagc cgccatgcta cggcaatccc ggcgaattag
     4981 ctggatctgc accgcaggcg aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga
     5041 acaacagccg ctgtttaata aacggttgcg ccgcaatcgc cagctctgtg ccctgcaatt
     5101 aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac gagcccccaa
     5161 cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga gcatcgctga
     5221 tgaacaaaaa ctttgttatg gcctgttggg actggaaccg ttaagtcgcg gtcgtgcatg
     5281 ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga gccatgagga
     5341 acatgcgcta cgcttgcgcc agtctctgga gcgtttgcga gtggtgtgtt ggccttggca
     5401 aggtgcggtg gcactgaaag aacagcaccc ggaaatgact caatatcata ttattcaaaa
     5461 ctggctgtgg ctgggggcgg ttaattcgct ggaagaagcg acaacgttaa ttcggacacc
     5521 cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt ccggtaacta
     5581 tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc actgaacaac
     5641 agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca gcacacgccc
     5701 tggtcggtag tgagcagttt gtcgcccagt tgccacgccc cgttaatcac aaacaccacc
     5761 ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt cgccttacag
     5821 acatccaggc gcgtcataat attgaaatcc atcgacatct gccctgccgt cagtttcgct
     5881 tttaccacct ggtccgccgt aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag
     5941 cggtctgcgc tttcaaggag catctcgccg ccttccagca acgtcactat cctttccatg
     6001 ccgggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc acgccagtaa
     6061 aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc agcgtttcgc
     6121 cacagattca ccgacatttt acgcatatca aagtattcca tactcgctcc cggcccgttc
     6181 ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg tgatttacgt
     6241 tagtggtgat caggcttttt ttacaactgt ttgagaagag aaaagaaaac cgccgatcct
     6301 gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa
     6361 agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg tcagacgctt
     6421 ctcaaaatta gcattaaatt gctttttctg ttccggcgtc aggatgttgt aaattttgtt
     6481 ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt tcttccattt ttgcgatctg
     6541 cgcttcagct tttgctttat cgaaggtatc gctggcaatg atgtcatgca ttgcgcggcg
     6601 ttcttccagc ggcggacgtt tcatctggtc acgctggcct ttcatgattt cgtggatctg
     6661 ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat gcggaccgaa
     6721 cttgcctttg tggtgcatca tcggcttcgc gtcagccggt gctgcggtag tggtgtctgc
     6781 ggcatgggcc aggttagccg cgccaagagc cagggtagag gcaacaaaca gtgcagttaa
     6841 tttacgcata ttctatatcc ttcctttcag ttatttatta cggctttctt aagtagcgtg
     6901 ccgtgttgac gagattaact ttactgactt tagcgtcaat taatcagagc aacggtaaaa
     6961 caatgaaagt gtaaaaaaca cttttgcgcc aattatggag aaaaaaagaa aatttgatgg
     7021 agagtgatga gagaatatta caacacgatg attttgcaga gattatgaag aactataccg
     7081 gatgactggt gataaataaa gcaaataacc aggattaatc tgtattaatt tataagaaag
     7141 caacttaata cccgcagaat gatttctgcg ggtaagtatt agcttatttt ttcgagcatt
     7201 aatcccgcgc gtaatcccaa cgctaccaac ggattaggga ataacacata ctctacatca
     7261 tgggttacgg taaaacgttc ctctccgtcc tgcgccagca atgttccttt ctcaaacggc
     7321 ataaaattca gcgtgtcact tgccatatgc atttcgaagg acggcgagtg gcgagtaatt
     7381 tgcgaaacca cccgataacg gagcggcggt gttctcacga taccgacact ctcaccagat
     7441 agcagcgcag caattgcgct ggcagttact gcaaactggc gaagatcgtt ttgcccaaag
     7501 ggcaacgctt tgccaagttc caacgtacag gccagcgcgc caaaatgtct gacgctgaaa
     7561 tgggtaaacg taccaccagg ctcctgatgg aacaccagcg cctccagccc cgccgcaccc
     7621 agccacgtca gaaatttctc gtcccaggga atgtcgcgtt gcggtaatac accgaactgc
     7681 ggatgcaagg agccacgaat tgcggtatgt agatcaaggt gccaacgcac agattctttg
     7741 ccctggtcat aaaaatcttc caggcactgt tccagttcgc gcgcccgaca ggtttctccg
     7801 ctttcagcaa atagctgcca acgaccgcca aacattcggt tcatatcgct atggcaataa
     7861 cgtttccctt gcttcagcgc aggaggattc ccgaggatca ccagcaaccg ccaacgtaac
     7921 gggatctcgc cgtgagatat cgcgccaagc aacgcgtcca gcatttccac aggtgccgtc
     7981 tcattaccgt ggatccccgc agaaatcacc agcgcgcctt gcggtggcgt taatggcgtc
     8041 agttccagca caccatcgcc cagccagcgc cagcgaacgc cgttgatttc tcgctcggtg
     8101 ataaccggtt ttttacccgt taaggtcaga gcaagaaaat tatccattgc cccctccctc
     8161 gcgctggaac ggataaaccg aaccgagatt cagtaattgg ctcaatacat ccagtgcttc
     8221 ccgcccttcg cgcagcaatt gcgggtcggc cagatcggca gcagtaagac gatcgcggta
     8281 gtaacgatcc acccagtcat tgagcgcatt aaacaaggta tcgttcatca tcaccgccgg
     8341 attcaccgcc cggcgttctt cttctgtcaa taccacccgc aaccgcaggc acgccgggcc
     8401 gccgccattc gccatgcttt cacgtaagtc aaagactttt agttcgctaa tcgggttgtc
     8461 agcggcaagg agttcattga gataacccca tactccggcg tgttcccgac actcctgagg
     8521 cagcaccaac atcatggaac catcatcgcg gctcaacagt tggctgttaa acagataggt
     8581 agacaccgca tccgacacgg aaacctgagt tgccggaact tctatcgcca taaaaccgtt
     8641 cacccgcgtg ccagtaactg tgactggcga gcgaacgcct gttggtggca aaacagcacc
     8701 tggcggttac tcacggcaat cacgtcatta tgaaaaacgc tctggtcgat aacgtccggg
     8761 ttttgctggg cgaaaatcac ctgttgggga ttcacctgat taagccttgc caccgcctcg
     8821 ctggcttcgc gagtctgtcg cgccggataa cgggaaggcc gggtatcatt gccttcttct
     8881 cgcccgtaga caaaaagtag catacccggt tcaccgtaat gaccgccgag acgattgtgg
     8941 tttgccgccc cctcatcacc gagcaacgct acctgcggca acgccgaatg gacgctaaat
     9001 ttctcttcgt ccttaaaaat cgcttttaac agcgattcag tgacgggcgc ttccagcgaa
     9061 cggtgaaatt tattgttcag gttggcaacg gtgagatgca ctttgccatc cagcgtatcg
     9121 gcagatggcg cgatcgttgc cgcattggct acccacattg gcgaagcgga actgacgctg
     9181 gaaagccagt gcggtgcctg gcgtgcaact ttttccagta cctgctcatc gctaccactg
     9241 aatcccaact gacgcagcac cggaataaac ggacgctcgt gcggcggaat cacggcctgg
     9301 gggaatcccg catcggcaag ggctttcatt ttcagtaagc cctgttttgc cgccagtcgc
     9361 gggttagaca cctgaaaacg gtgacgggta gaggcttcat taccaaacga caggcccgcg
     9421 taatgatgcg tcagccctac cagcccgtcg aaattaactt cccaggcgtt catcgcacca
     9481 cctcatcgga aaaatccagc ccggggttaa gcgtggcggg caatgttaac gagtccgact
     9541 ccaggctcgc cataggccat gcgcagtaat ctgcggcata ccaggcgctg gggcgatggt
     9601 taccggaagc accaatgccg ccgaatgggg cggtactggc agcaccagta agcggtttgt
     9661 tccagttaac aatccccgcc cgcgcctcca gcaacagttg atcgaatttt tcccgctcgg
     9721 gggaaaccag accgcaaaag agtccgaagc gagtgttgtt cgccatccga atcgcctcat
     9781 caaaagtatc ataacgccag acgcgcaaaa acggcccgaa cacctcttca tccggcacac
     9841 cgccaacgcc cgtcatttca atgatccctg gtgtcagcaa cgatgtccct gcttgtaata
     9901 agcgcggcgc aagcagggtt cgtccgccca tcgcttccag ttgctgccag gcagtaacca
     9961 cctgctgtgc ggcctgttca gaaatcagcc cgccaataaa cggctgcggt tcgtcatccc
    10021 agttgcccgg cgttaatcgc tggctgacgg caaccagact agcaagaaac gcatcgccct
    10081 gcgctccgct tttgagcaat aaacggcggg cgcaggtgca gcgttgcccg gctgtgacaa
    10141 acgccgactg aatggtcaga tggacagccg cgtcgatatc cgccacctca tcgataatta
    10201 gcggattatt accgcccatc tcaagggcga gaattttctc cggctgaccg gagagctggc
    10261 gatgcagctg gtagccggta ttggcgctac cggtaaacag caaaccgtcg agatcctcca
    10321 gcgcactcag cgcctgaccc gtttcacgcc cgccctgcac caggttcagc acgcccggcg
    10381 gcaagccagc ctgctgccat aaacgcatta ccgcttcgcc actccacggt gtcagttcgc
    10441 tgggtttaaa gataatggtg ttacctgcca gcaatgccgg aacgatatgt ccgttcggca
    10501 aatgaccagg gaaattatac ggcccaaaca ccgccagcac gccgtgcggg cgatgtcgca
    10561 ggctcgccgc accgtccggc atttcactac gctgctcgcc ggtacgaacg tgatacgcct
    10621 taattgatat cgcgatttta ttgatcatcg ccgtcacttc ggttgccgct tcccagcgcg
    10681 gcttacccgt ttctctggca ataatcgcgg ttaattcggc tttattgctt tccagcaatc
    10741 cggcaaagcg ttcgacaacg acctgacgtt cagccaatga gagccgcgcc cagcgcggaa
    10801 acgccgcacg ggctgcccga caagcctgtt cgacctgagc ggcatcggca tcattgcctt
    10861 gccataacac ctcgcccgat accggattac gcttcacacg cgatgcgccc tggcccgtta
    10921 tccagtcacc gttaatccat aaagtcatgc tgttttctcc tctgcgcaca ggcgcaccag
    10981 acgaacgcga tcccctgcgt ggcatttgag ggcatccagt tgtgcggcgg ttaaaatcaa
    11041 acgctcggtt gccggatcgg tacgcgccag caccacgcgg aaatggtgat aattttcatt
    11101 ggcgaccagg caggccggaa aatcgccctg cgcaggctgc ccttctgcca cttccaccag
    11161 ccgactttta cggatggcgc gcacgcggtc gatgtcacac tcaagcgttg gcccgccgtc
    11221 gaagatgtcg atatagttgc ggtaacgaaa accttctttc tccagcaccg cgcgggcagg
    11281 cgcggtttgc ggatgtacct gaccgatgac gtcctgggct tcctgggata aaaagtgggt
    11341 atagatcgga tgtttcggca tcagttctgc aataaatgcc ttttgcccgg tgccgcagag
    11401 aaaatcggcg cggctaaaat ccatcgaaaa gaagcgttta ccgaggcttt gccagaacgg
    11461 tgaatagccg tgttcgtcaa tcaccccgcg catttcagca accactttgt cattaaactt
    11521 gtcgcgaaaa gccgccataa acataaagcg cgatttcgat agcaaatagc cgttgccctc
    11581 tttgcgccag tccgggtcga gaaacagcgt gcacagctcg ctgctgccgg tgtgatcgtt
    11641 actaagaaac agcgtcggca atgcgttata gacattcagc tcttttgagg cgtgaaccaa
    11701 cgtgccgacg cgatagttgt accagggatc gttcagccca accgccacct caatagcgca
    11761 aatccccgcc acggtgcctg tctcgctatc ttccagcacg aacacatagc cctgctcgct
    11821 tttgggcagt tcaccttgcc aggttttgat tgccctttcg atacgcgccg aaagcgtggc
    11881 ttcattggcg ggaagcgacg tcaggccgcc gcccgttttg ctggcaagct gcatcagcgc
    11941 cgagacatct gagcgctcaa cgggacggat gaccatcatg atgaacctcg gctaacaaag
    12001 tgttcgcaag cagctgcaaa gcgatccagt ccggtcgtca cctcttcttc gctgacattg
    12061 agcgcaggcg caaaacgcac cacgttgcca cccgcaatca gtaccatcac gcctgctttc
    12121 gccgcttcca gagagatctg tttcgcttgc ccggcgtaat cggcattcag tacacagcca
    12181 atcaacaaac ctaagccgcg aacttcactg aacaaaccat agcggtgatt aatagtatta
    12241 agacgctcaa caaaccagtc gtgacgctgt ttaacgccat taagcatctc tggtgtgttg
    12301 atgagctcca gcactttgcc tgctaccgcc gaggccagcg gattaccgcc ataggtggtg
    12361 ccatgagtgc caacggtcat cacgcgggcg cactcttcgg ttgccaacaa cgcaccgacc
    12421 gggaaaccgc cgcccagcgc tttggcggtg gttaacagat cgggcgtcac gccgtagtgc
    12481 atataggcat acagctcacc agtgcgcccg acgccggttt gtacctcatc aaaaatcagc
    12541 aaggcattgt ggcggtcaca caattcacgc agaccttgta aaaacgcgtt gctggctggc
    12601 accacaccgc cttcgcccta gatgggctcg acaatcaccg cacaggtggc gtcgtcaatc
    12661 agcgcgctgg cagagttaat atcgttatat gcagcatgac gaatatccgc cggcagtggc
    12721 gcaaaatcct gtgaataggc tggctgccca cccgcactga cagtaaacag cgtgcgacca
    12781 tgaaacgcat ttttgaacgc cacgatgcca ctcttatggc tgccgtagcg gtcgtgagcg
    12841 aatttacgcg ccagttttag cgccgcttcg ttggcttccg caccggagtt acaaaagaag
    12901 acgcgatcgg caaacgtggc gtcgatcaat tttttcgcca gtcgcagtac cggctcgttg
    12961 gtgtaaccgt tgccggtatg ccagaactta ctcgcctgtt cgttcagcgc ttcacgcagt
    13021 tccggatgcg catggcccag cgcgttcacc gcaatgccac ccgcgaagtc gatatactct
    13081 ttcccctgct gatcccacaa gcgcgaacct tcgccacgta ccggtataaa gggtgccgga
    13141 gcgtaaacag gtatcatcca ttcatcaaag ttttcacgcg taattggctg agacatagcg
    13201 acctctacag taaataattc gttatttgta tgttaacaat aagtaatgtt tgcgctgtaa
    13261 atgtagattg cagggttcgt gccagccagt gataaaagtg cataaacggc ggaggctaac
    13321 tggaaatcaa ggagttataa ccaaaccata tgcatttaaa gtgcatataa agtgaatacg
    13381 tttgcgatgt gggtgaataa aaagaataaa aaacgcaatg ttatgcagaa gtaaaatata
    13441 attctagaat tgtgatcatt gacgaaattt actggaaatt actgcgccat tctgacgcag
    13501 cgcgcaccaa aagcgggcat tttttgcgcc atcgttgaca tcattaacaa ccatcgatca
    13561 aatcacttaa caacaggcgg taagcaacgc gaaattctgc taccatccac gcactcttta
    13621 tctgaataaa tggcagcgac tatgaaattt gtctctttta atatcaacgg cctgcgcgcc
    13681 agacctcacc agcttgaagc catcgtcgaa aagcaccaac cggatgtgat tggcctgcag
    13741 gagacaaaag ttcatgacga tatgtttccg ctcgaagagg tggcgaagct cggctacaac
    13801 gtgttttatc acgggcagaa aggccattat ggcgtggcgc tgctgaccaa agagacgccg
    13861 attgccgtgc gtcgcggctt tcccggtgac gacgaagagg cgcagcggcg gattattatg
    13921 gcggaaatcc cctcactgct gggtaatgtc accgtgatca acggttactt cccgcagggt
    13981 gaaagccgcg accatccgat aaaattcccg gcaaaagcgc agttttatca gaatctgcaa
    14041 aactacctgg aaaccgaact caaacgtgat aatccggtac tgattatggg cgatatgaat
    14101 atcagcccta cagatctgga tatcggcatt ggcgaagaaa accgtaagcg ctggctgcgt
    14161 accggtaaat gctctttcct gccggaagag cgcgaatgga tggacaggct gatgagctgg
    14221 ggggtggtcg ataccttccg ccatgctaat ccgcaaacag cagatcgttt ctcatggttt
    14281 gattaccgct caaaaggttt tgacgataac cgtggtctgc gcatcgacct gctgctcgcc
    14341 agccaaccgc tggcagaatg ttgcgtagaa accggcatcg actatgaaat tcgcagcatg
    14401 gaaaaaccgt ccgatcacgc ccccgtctgg gcgaccttcc gccgctaatt tagcagctct
    14461 cctggctcaa actgggtcag gagaattaac cttgagaaaa atcaacaaac tgtcagtaat
    14521 gatttgttgc ctgccgtcct ttgttatacc gtcactgcgt ttttagttgt ctgaccactt
    14581 ctctattatc aagtttgata taggaaactc cacgatgaac gctgagcgta actttctgtt
    14641 tgcctgtctt atttttgcgc tggtcattta cgctatccac gctttcggtt tattcgatct
    14701 gctcaccgat ttaccccact tacagacact cattcgccag agcggattgt tcggttatag
    14761 cctctatatt ctgttattca tcaatgcccc cctctttctg ttaccaggaa gcatactggt
    14821 gatcgctgga ggaatagttt ttggcccgct cttagggaca caactctcat taattgccgc
    14881 aacgctggcc tcctcgtgct cattcctgct ggcgcgctgg ctgggacgtg atttactgct
    14941 gaaatacgtg ggccatagcc ataccttcca ggccattgaa aaaggcattg cgcgtaacgg
    15001 tattgatttt cttattctga cccgcttaat cccgttgttt ccttacaata ttcaaaatta
    15061 cgcttacgga ttcaccacca tcgccttctg gccttatacc cttatttcgg cactcacgac
    15121 cctgcccggt attgttattt ataccgtgat ggcaagcgat ctcgccaatg aaggcattac
    15181 gctgcgcttt attttacaac tctgtctggc gggcctggcg ctgtttattc tcatccagct
    15241 cgcaaaactc tacgcccgac acaaacatgt ggatctgtct gcttcgcgcc gcagcccact
    15301 tactcaccca aaaaatgaag gatagaacga tgtcgcaaca ttattcagtg tcatggaaaa
    15361 aaggactggc tgcactctgc ttactggctg ttgccgggct tagcggctgc gatcaaaaag
    15421 agaatgcggc ggcaaaagtg gaatacgacg gactttcgaa cagccaaccg ctgcgcgtcg
    15481 atgccaataa ccatacggta accatgctgg tgcaaattaa tggtcgtttc ctcaccgacg
    15541 acactcgtca cggtattgtg tttaaagatg gctccaacgg acataaatcg ctgtttatgg
    15601 cttatgcgcc cccgaaagca ttttatgaag ccctgaaaga ggcaggtggt acgccgggcg
    15661 aaaacatgac gatggataat aaagaaacga ctcatgtcac aggcagcaaa ctggatattt
    15721 cggtcaactg gcaaggggcg gcaaaagcgt attccttcga tgaagtgatt gttgacagta
    15781 atggcaagaa actggacatg cgctttggcg gtaatttaac ggcagcagaa gagaagaaaa
    15841 cgggttgcct ggtgtgtctg gatagctgcc cggtcggcat cgtcagcaat gcaacataca
    15901 cttatggtgc ggtggaaaaa cacggcgaag tgaaattcaa aggcaatgcc tcagttctcc
    15961 cggcggataa cacgctggca acggttacct ttaaaatcac cgaataaagc caagataaag
    16021 gatgatgaag atacaatcgc gtaaaatctg gtactaccgt ataaccctca tcatcctgtt
    16081 gttcgctatg ctgctcgcat gggcgctgct tcccggcgtc catgagttta tcaatcgcag
    16141 cgttgcggcg tttgccgccg tggaccaaca gggtatagaa cgctttattc agtcttacgg
    16201 cgcactggcg gcagttgtct cgttcttgtt gatgatttta caggccattg ctgcaccgct
    16261 acctgcgttt ttgatcacct ttgccaatgc gtcgctgttt ggcgcgttct ggggcggctt
    16321 gctgtcgtgg accagttcga tggccggcgc ggcgctgtgc ttttttatcg ccagagtgat
    16381 gggccgcgaa gtggtggaaa aattaaccgg caaaaccgtg cttgacagta tggacggctt
    16441 tttcactcgc tacggcaaac acaccatcct ggtctgtcgg ttattgcctt ttgtcccttt
    16501 cgatccaatc agctatgctg ccggtctgac ttcaatacgt tttcgctcgt tttttatcgc
    16561 caccgggctt ggtcaattac cggcgactat tgtttattcc tgggcaggca gcatgttaac
    16621 aggcggtact ttctggtttg tcaccgaact gtttattctg tttgccctga ccgtggtgat
    16681 ttttatggcg aagaaaatat ggcttgagcg ccagaagagg aatgactgat gggtttaccg
    16741 ccgcttagca aaattccttt cattttacgt ccgcaggcgt ggctgcatcg tcgccattac
    16801 ggcgaggtgc taagcccgat tcgctggtgg gggcggatcc cgtttatctt ttatttggtg
    16861 tcaatgtttg ttgggtggct ggagcgcaaa cgctcaccgc tcgatccggt agtgcgatcg
    16921 cttgtcagcg cgcgcattgc gcaaatgtgc ctgtgtgagt tttgcgtgga tatcaccagt
    16981 atgaaagtcg ccgagcgtac cggcagcacc gataaactgc tggcagtggc tgactggcgg
    17041 caaagcccgc tctttagcga tgaagaacgg ctggcgctgg agtacgccga agccgcaagc
    17101 gtcacaccgc caacagtcga tgatgccctg cgtacccgac tggctgcgca ttttgacgct
    17161 caggcgctca ccgaactgac ggcattgatc ggcctgcaaa acctgtcggc tcgttttaat
    17221 tctgccatgg acattcccgc tcaggggctg tgccgtattc cggaaaaacg ttcttaagga
    17281 gggaagatgc gccattgtgg gtggttgctg ggattgttat cgctgttttc tttggcaaca
    17341 catgccagtg actggcagga aattaaaaat gaggccaaag ggcaaaccgt ctggtttaac
    17401 gcctggggcg gcgataccgc aattaaccgc tatctcgact gggttagcgg cgagatgaaa
    17461 acccattacg ctataaacct gaagattgtt cgtctcgcgg atgccgcaga cgcggtgaag
    17521 cgcattcaga ccgaagctgc tgccggtcgt aaaacgggcg gctcggtgga tctgctctgg
    17581 gtgaacggcg aaaacttccg caccttaaaa gagtaatgcc agtcagttaa gcaact
//
LOCUS       DAJTRD010000081        17466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTRD010000081 DAJTRD010000000
VERSION     DAJTRD010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.081"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(43..774)
                     /gene="rlmB"
                     /locus_tag="OO315_002977"
     CDS             complement(43..774)
                     /gene="rlmB"
                     /locus_tag="OO315_002977"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5891805.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(954..3395)
                     /gene="rnr"
                     /locus_tag="OO315_002978"
     CDS             complement(954..3395)
                     /gene="rnr"
                     /locus_tag="OO315_002978"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5891806.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3434..3859)
                     /gene="nsrR"
                     /locus_tag="OO315_002979"
     CDS             complement(3434..3859)
                     /gene="nsrR"
                     /locus_tag="OO315_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5891807.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4064..5362)
                     /gene="purA"
                     /locus_tag="OO315_002980"
     CDS             complement(4064..5362)
                     /gene="purA"
                     /locus_tag="OO315_002980"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5891808.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5466..5663)
                     /locus_tag="OO315_002981"
     CDS             complement(5466..5663)
                     /locus_tag="OO315_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5891809.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5745..6749)
                     /gene="hflC"
                     /locus_tag="OO315_002982"
     CDS             complement(5745..6749)
                     /gene="hflC"
                     /locus_tag="OO315_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5891810.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6752..8011)
                     /gene="hflK"
                     /locus_tag="OO315_002983"
     CDS             complement(6752..8011)
                     /gene="hflK"
                     /locus_tag="OO315_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5891811.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8097..9377)
                     /gene="hflX"
                     /locus_tag="OO315_002984"
     CDS             complement(8097..9377)
                     /gene="hflX"
                     /locus_tag="OO315_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5891812.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9453..9761)
                     /gene="hfq"
                     /locus_tag="OO315_002985"
     CDS             complement(9453..9761)
                     /gene="hfq"
                     /locus_tag="OO315_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5891813.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9847..10797)
                     /gene="miaA"
                     /locus_tag="OO315_002986"
     CDS             complement(9847..10797)
                     /gene="miaA"
                     /locus_tag="OO315_002986"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5891814.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10790..12637)
                     /gene="mutL"
                     /locus_tag="OO315_002987"
     CDS             complement(10790..12637)
                     /gene="mutL"
                     /locus_tag="OO315_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5891815.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12647..13984)
                     /gene="amiB"
                     /locus_tag="OO315_002988"
     CDS             complement(12647..13984)
                     /gene="amiB"
                     /locus_tag="OO315_002988"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5891816.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14003..14464)
                     /gene="tsaE"
                     /locus_tag="OO315_002989"
     CDS             complement(14003..14464)
                     /gene="tsaE"
                     /locus_tag="OO315_002989"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5891817.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14436..15983)
                     /gene="nnr"
                     /locus_tag="OO315_002990"
     CDS             complement(14436..15983)
                     /gene="nnr"
                     /locus_tag="OO315_002990"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5891818.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15982..17121
                     /gene="queG"
                     /locus_tag="OO315_002991"
     CDS             15982..17121
                     /gene="queG"
                     /locus_tag="OO315_002991"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5891819.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17104..17157)
                     /locus_tag="OO315_002992"
     CDS             complement(17104..17157)
                     /locus_tag="OO315_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891820.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17391..17466)
                     /locus_tag="OO315_002993"
     tRNA            complement(17391..17466)
                     /locus_tag="OO315_002993"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17431..17433),aa:Gly,seq:gcc)
BASE COUNT         3962 a         4983 c         4388 g         4133 t
ORIGIN      
        1 aaaaccatcc aaatctggat ggcttttcat aattcagagg aactagctgc gctgacgaac
       61 cgcttcaaat aagcaaattc cggttgcaac cgaaacgttc agggaagaaa cacttcctgc
      121 cattgggatg ctgatcaact catcgcaatg ttcacgggtc aggcgacgca taccttcacc
      181 ttccgcgccc atcaccagcg ccaggcgtcc ggtcattttg ctctgataga gtgtatgatc
      241 cgcctcgcct gccgtaccga cgatccagat attctcttcc tgcaacatac gcatggtgcg
      301 cgcaaggtta gtcacccgaa tcagcggaac gctttctgcc gcgccgcagg ctactttttt
      361 cgccgtagcg ttgagctgtg cggagcgatc tttcggcaca atcaccgcat gaacgcctgc
      421 ggcgtccgca ctacgcaggc acgcgccgag gttgtgcgga tcggttacgc cgtcgaggat
      481 cagcaggaac ggttgatcga gcgaagcgat cagatccggc agatcgtttt cctgatactg
      541 acgtcctggc ttcacgcggg cgataatgcc ctgatgcacg gcaccgtcgc ttttctcgtc
      601 gagatattgg cggtttgcca actggataac cacgccctga gactcaaggg cgtgaatcag
      661 cggtaacaga cgtttatctt cacggccttt taaaataaag acttcctgaa aacgttcagg
      721 ggcgcgctcc agcagggcct gcactgcgtg gatgccgtaa atcatttcgc tcattaatgt
      781 actcgttgtt tacgttttgc ctgatgcact acgtttatcc ggcctacatg atctctgcaa
      841 tatattgaat ttgcgtgatt atgtaggccg gataaggcgt tcacgccgca tccggcatga
      901 acaaagcgca ttttgtcagc aatctaaccc tcttctttta aagagggtat tgatcactct
      961 gccacttttt tcttcgccgc acgcttcgct ttggtcgctg cggctatttt ctgcgttttc
     1021 gccgatggct ttttcgcttt tctcgcgtct ttcttcgccg ctttcggctt cgtttttttc
     1081 tcaccgcgga aggcgctgtc tggctcaaag tttacctttt taccgacctg acaacgcttg
     1141 ccgccttttt tgcctgcatc gccttttttc gctttctcgc gcgccgtttt accgacgttg
     1201 cgcggtgcgc gttcactgga gatcagacta aagtcgattt tgcgctcgtc catattaacc
     1261 gcttcgacgc gaacttccac gcgatcgccc aggcgataag tctggccgct ggattccccc
     1321 atcaggcgtt gccctacctg gtcaaagcga tagtagtcat tgtccagcga agagacatgg
     1381 accagaccat caatgaacaa gtcgtccaga cggacgaaga agccaaagcc agtgacgctg
     1441 gaaattacgc ctttaaagac gttacctacc tggtcgagca tgaagtcaca cttcagccag
     1501 tcagcgacat cgcgcgttgc ttcgtcggca cgacgttccg ccatcgaaca gtgctgaccc
     1561 agttgcaaca tctcttccat cgaataatgg tagccgccgg tttcagtggt gttgccctga
     1621 tgcccctgct ctttcgccag cagatattta atggcgcggt gcagcgtcag gtcaggataa
     1681 cgacgaatcg gcgaagtaaa gtgcgcatag gactgcaatg ccagaccgaa gtgaccacgg
     1741 ttttctggat cgtaaatcgc ctgtttcatc gagcgtagca gcatggtttg cagcatttct
     1801 gcatcaggac ggtcggcaac cgactccagc agttccgcgt agtcacgcgg ttccggcttg
     1861 ttaccacccg gcagctccag ccccagctcc gccagcactg aacggaaaga ggtaatcgct
     1921 tcggtgctcg gcttgtcgtg aatacggaac agtgccggtt ctttcgcttt ctcaacgaaa
     1981 cgcgccgccg agatattcgc cagaatcatg cactcttcaa ttaatttgtg cgcgtcgtta
     2041 cgctgggtct gttcgatacg ttcaatacgg cgttcagcgt tgaaaatgaa cttcgcttct
     2101 tcgctctcaa atgagatccc accgcgttct tcacgggctt tatccagcac tttatagagg
     2161 ttatgcaact cttcgagatg cttaaccagc ggggcgtact gctcgcgcag atcctgatcg
     2221 ccctgcagaa tatgccagac tttggtgtag gtcagacgcg cgtgagagct catcaccgct
     2281 tcgtagaatt tgtagcccgt caggcggcct ttcgacgaaa ccgtcatctc gcacaccata
     2341 cacaggcggt ctacctgcgg gttgagcgaa cacaggccgt tagagagcac ttccggcagc
     2401 atcgggataa cctgcgaagg gaagtacacc gacgtgccac ggttacgcgc ttctctgtcc
     2461 agcggcgttg gcggacgcac atagtagctg acgtcggcaa tcgcgaccca taaacgccag
     2521 ccgccgccgc gttttttctc gcagtaaact gcatcgtcaa agtcacgggc gtcttcgcca
     2581 tcaatggtga ccagcggtaa atcgcgcaaa tcgacacggc ccgcttttgc ttcttccggc
     2641 acttcttctt tcagccccgc aacctgttgc tcaacagcct gcggccagat atacggaatt
     2701 tcatgggtac gcagagcgat atcaaccgcc atgccggtgc ccatattgtc gcccagcact
     2761 tcgacgattt tacccaccgc tttggtgcgg cgagtcggac gctgagtcag ttcgactacg
     2821 accacaaagc ccatccgcgc gcccatgatc tgatcgggcg ggattaagat atcgaagctc
     2881 agacggctat cgtcaggaac cacaaagccg acgcccgctt cggtaaagta gcgaccaaca
     2941 atctggctgg tttttggcac cagtacgcgg acaatacgcg cttcacgacg acctttacgg
     3001 tcagcaccca gcggctgcgc cagcacctga tcgccatgaa tgcaggtttt catctgctcg
     3061 ctggagagat acaaatcatc tttacgccct tcaacccgca gaaagccgta gccatcacgg
     3121 tgaccaataa cggtaccttt caccaggtcg aggcgttccg gcagcgcata gcactgacga
     3181 cgagtgaaga ccagttgacc atcgcgctcc atcgcgcgca ggcggcgacg caggccttca
     3241 agctgctctt cgccttcaat gtgcagttct accgccagct catcacggct ggccggtttt
     3301 tcacgtttgg ttaaatgttc gaggataaat tcccgactag ggatgggatt cgcgtatttt
     3361 tcagcttcgc gttcctggaa aggatcttgt gacatctcgg ttcctccgtt gtcatctctg
     3421 atgaagattt tcgtcactcc accagcaata atttataaag cggttgattc tcttcaacca
     3481 aatcggcaag cgtgtagtta tccagttccg taagaaaact ttgcacggcc ttagaaagcg
     3541 cctgtttcaa ccgacaggca ggtgtaatgt ggcaaaactc actgctgcaa ttcaccagcg
     3601 ataagggctc cagctcgcgc accacatcac caatacgtat cgcactcgcc ggtttaccca
     3661 ggcgaatgcc gccatttttt ccacgaacag cagtcacgta gccggcacga ctaagttgat
     3721 tgattatttt gaccatatga ttacgggaga cgccgtagac gtcagtcact tcagaaatac
     3781 tggtcatccg cccttctggc aatgacgcca tgtagatcag cgcacgtaat ccgtaatcag
     3841 tgaaactcgt taactgcaca tcaacctcaa aagggaaatc gggaaaaaat acatttatat
     3901 tgatgataaa ccagccacaa gctgtgtcgc taatttattt cagttcgggg aaggaaaaaa
     3961 gcgaggattt aacactgtgt cggatagcgt aatggcttaa ccgacacagc aatgtatcac
     4021 actgttagcc cggcaggcaa aatatctgcc aggcgtacca gaattacgcg tcgaacgggt
     4081 cgcgcagaat catggtttca gtacgatccg gaccggtaga gatgatatcg atcggcacac
     4141 cggtcagctc ttcaatacgc ttgatgtagt tcagcgccgc ctgcggcagg ccgctacgat
     4201 ctttcacgcc gaaagtggat tcagaccagc ccggcatggt ttcgtaaatc ggctctacac
     4261 ctttccagtc gtcagctgcc agcggagtgg tagtcacttc gcgaccatcc ggcatacggt
     4321 aagccacgca gagtttaacc tctttcaggc catccagaac gtccagttta gtcaggcaga
     4381 agccagacag ggagttcagc tgtaccgcac gacgaacggc aacggtgtcc agccagccgg
     4441 tacgacgacg acgacccgta gttgcaccga attcgttacc ctgcttgcag aggaactcgc
     4501 cagtttcatc aaacagttcg gccgggaaag gacctgcacc tacacgagtt gagtaagctt
     4561 tgaggatacc cagaacgtaa tcaacataac gcgggcccag gccggaaccg gtcgccacgc
     4621 caccagcagt ggtgttggaa gaagttacgt acggataagt accgtggtcg atatccagca
     4681 gcgtaccctg cgcaccttcg aacatgacga aatcgccacg ctgacgcgcc tggtcgagca
     4741 ggtcagaaac gtcaaccacc atagaagtca ggatgtcggc aacagccatc gtatcatcca
     4801 gaactttctg gtaatcaacc gcttcagctt tgtagtagtt aaccaactgg aagttgtgat
     4861 attccatcac ttctttcagt ttttcagcga aggtttcttt gtcgaaaagg tcgccaacac
     4921 gcagaccgcg acgtgccact ttatcttcat aagcaggccc gataccacga ccggtggtgc
     4981 cgatcgcttt cgcgccacgc gctttctcac gcgcgttatc cagagcaacg tgataatcaa
     5041 ggatcagcgg acatgcttca gacagcagca gacgctcacg aacggggatg ccacggtctt
     5101 ccagttcttt catctctttc atcagcgcgg ccggagacag cacaacaccg ttaccgatga
     5161 tgctggttac attctcgcgg agaatacctg atggaataag atggagaacg gttttttcac
     5221 cgttgattac gagagtatgg cctgcgttgt gaccgccctg gtagcgtaca acatatttag
     5281 cccgttcagt cagaagatcg acgatcttac ctttaccttc gtcaccccat tgggtgccca
     5341 gtacgacgac gttgttaccc atttttcaaa atcaccgttt gcttaaaaat ggattctacc
     5401 atcgcttttt cagagttaca gcactttttg cactcaaaaa tgacctcgat cagtctgttt
     5461 tttgttcagc caatcgtttt cctcaacatg tagtagacca caacgcccgc aaccacaagt
     5521 ccaccgccaa aacgacgtaa aatattatcg ggcaaattgg tcatcgcaga gatcatcttc
     5581 ttccatgcct tcgggtaaag catcggccct aaaccttcca gtaccaaaac cagggcaagc
     5641 gccagccaga ttgtcgaatt cattcattat ccttatagaa aaagaaaacc accgacatcc
     5701 ctgaggatgc ggtggcttta ttgacctgta ccgcagtcgt tatattaacg cgttgcggaa
     5761 gtcggcgtct tcatgtagcg gaagaaatcg ctatccgggc tcatgaccat cacgtcctga
     5821 ttgccagaga agctgttctc ataagcacgc aggctacgga tgaatgcgta gaagtccgga
     5881 tctttactga atgcatcagc aaacagtttg gctgcttcgg catcgccttc accacgcata
     5941 atgcggccct gacgctcagc ttctgccagc gttctggtca cttcatagtc ggcagtcgcg
     6001 cgcagttttt ccgcttcttc ctgaccttgt gaacggtgac gacgcgctac cgcttcacgc
     6061 tcggcgcgca tacggttgta gatcgcttca gacacttcgg tcggcaggtt gatctgcttg
     6121 atacgcacat cgacaacttc aatacccagc gccgccatac tgttcgggtt gatgaccgga
     6181 actttgccct tcgtctcagc cgttacgcgc tctgccgctt cggcaatggc gttatctgct
     6241 gccggggtag taacttcatc ttctgtaccc gcagaaccgg agttcagcgc gtcacgtact
     6301 tcgagggtca gacgaccacg ggaatcggtg acgatatctt tcacgtccag gcgaccaatt
     6361 tcagaacgca gacggtcaga gaacttacgt ttcaacagca cttccgcttg cgaaatgtcg
     6421 ccaccacccg ttgccaggta gtaacggctg aaatcgctga tgcgccattt gatgtaagag
     6481 tcgacgatca ggtctttctt ctctttggtc acaaagcggt cggcctggtt gtccatggtc
     6541 tgaatacgtg cgtcgagcat tttcaccgtt tcaatgaacg gtatcttgaa atgcagacca
     6601 ggctcataaa ccaaaggttt gttgtcatcg tcacgcagta ccttaccaaa acgcagcgta
     6661 ataccgcgct caccttcttt gacgacaaag acagacatgt aaagcactac cagcacgatg
     6721 atgataatcg cgataactga cttacgcatc gttattcccc ctgacgctgg tagtcgttac
     6781 gctgcgcgtt ggcgcggcgt tggtccataa tatcgccctg actggtggac gacgtgttgc
     6841 ttgctccact ggttgtggag gaagaggctg gcggcagacg cagcagattg ctggcaccgt
     6901 tatcgctctt cgccgcaggg gcgttaccac ctttcagcat ctggtctaac ggcagaacca
     6961 tcaggttgcc acctttatcg ttaaccagca ctttgcgggt gttacccaac actttttcca
     7021 tcgtctcgat atacagacgc tcgcgagtaa tttccggcgc ggctttatat tccggcagaa
     7081 gtttagcaaa gcgcgccact tcaccctgag cttccaggat ggtctgggcc ttgtacgcac
     7141 gcgcctcttc gaggatacgt tgcgcctgac cgttcgcacg cggctgaact tcgttggaat
     7201 acgcttctgc ttcacgaatg tattgctgtt cgttttcacg cgcggcaatc gcatcgtcaa
     7261 acgccgcttt tacttcttcc ggcggacgag cagcctggaa gttgacgtcc agcagcgtga
     7321 tacccatgtc atacggacga atcgtctctt ccagttcgcg ctgagtatcg ctacgaatca
     7381 cggtacgacc ttccgtcaga atgcggtcca tggtgtattt accgataact ccacgcaggg
     7441 cgctgtcggt agcctgacgc aggctgtcat ccgggctggt cacgctatac agatattttt
     7501 ccggattggt gacgcggtac tgcacgttca tctcaacgcg cactacgttc tcgtccgacg
     7561 tcagcatcac accagaagcg gccagttcac gcacggcttc cacgttcacc ggtttgactt
     7621 cgtcgataaa cgtcggtttc cagttcagac ccggctcaac cagatggctg aatttaccaa
     7681 agcgtgttac cacgccgcgt tcggcttctt taatggtata gaaaccactg gccgcccaga
     7741 taatgacaat cgctgccgct gcgatggtaa cgacacgacc gccaagctgc gggcgcgggc
     7801 cttgtgatga actgccaccg ccagatccgg tgcctttacc gccgcccaga ccaccgagct
     7861 ttttgctcag tttgcggaag atatcatcta aatcaggtgg cccttgatcg cgaccgcctt
     7921 tgtttccatt tccctcagag ttgccgccag gtttgctgct tccccacggg tcgcggtctt
     7981 gtccgttatt accgggctga ttccacgcca tgtttgtgct ccatatttgt tatgcggtga
     8041 tccctgttgt ctttcgacgc cagaggatat gactccacgc ttcagacgct acgccgttag
     8101 atcaggtaat cgatcaacgc cggttcttgt ttacagaggc gacgccagtc aacgatcggc
     8161 atacgaactt gcagacttac gctgccgtcc tcctccatcc actctttttc tattgcctga
     8221 agctgataaa aacgactcct cagacgccct tcctgcggtg gcagacgcaa tgtatgctgc
     8281 gccacctcgc cggaaagccg ctccgtcaaa gcctgaaaaa gctgtggtat ccccgctccg
     8341 gtctgtgcgg aaagccagac acggatcggt ttgttctctt cgtcccgatc aatacgcggt
     8401 tcgaaatctt ccagcatatc gatcttgttc atcaccagca gggttgggat ctcgtgagcg
     8461 tcgatctctt caagaaccgt attcaccgct tcgatgtttt cttgtacacg cacatccgcc
     8521 gcatcaatga cgtgcagcag taatgtggct tgccgcgtct cttgtaacgt ggctttaaat
     8581 gccgccacca gatcgtgcgg caggtggcga ataaacccta cggtatctgc aagtacggtt
     8641 tcaccgacat ctgcaacgtc aatacgccgc aacgtcgggt cgagggtggc aaacaactgg
     8701 tctgccgcgt agacccgcgc ttcggtgatg cgattgaaaa gggtagattt accggcgttg
     8761 gtatatccca ccagcgaaac agtaggaacg tcggctttga tacgcgattg ccgcccctgc
     8821 tcacgctgct tttcaactct ttccaggcgc gactgtatct gcacgatgcg attacgcaac
     8881 aaacgacggt cggtttcgag ctgggtttca cccggaccac gcaaacctat cccgcctttc
     8941 tgtctttcaa ggtgggtcca gccacgcacc aggcgcgtag ccagatggcg cagctgcgcc
     9001 agctcaacct gcaacttacc ctcatgggta cgcgcacgtt gggcgaaaat atctaaaata
     9061 aggccggtgc ggtcgataac acgacactcg cacaaacgct ccaggttacg ctcttgcgcc
     9121 gggctcaggg catggtcaaa aagaacgacc gaagcaccca tcgctttgac agcttccgca
     9181 atttcaactg ctttaccttc acctacaaaa tactttgggt gcggcgcttt acggctaccg
     9241 gtaatcacct gcaatgcttc gacaccggcg gaagagacca gagattcaaa ctcctggagg
     9301 tcttccatat ctttgtcttg cgtaaaatag atgtgtacca gtaccgcctg ctcaccagca
     9361 tcataacggt caaacaagcg tataaccctc taaatagatc agcggggaac gcaggatcgc
     9421 tggctccccg tgtaaaaaaa cagcccgaaa ccttattcgg tttcttcgct gtcctgttgc
     9481 gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc gccaccggcg
     9541 ttgttactgt gatgagaaac cgggcgagat gggacaacag tagaaatcgc gtgcttgtaa
     9601 accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga ctcgatttgc
     9661 ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc ccgacgcagt
     9721 gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt tccttatatg
     9781 cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg tctcaattgt
     9841 acacattcag cctgcgatag caccaacaac ctgtaatact tcgtcacgcg cctgttctgg
     9901 tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta tctgccgctt
     9961 cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg agatttcgcc
    10021 ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca agtccgtatg
    10081 caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag ccaacatctg
    10141 atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg cgatggcgaa
    10201 ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg ttaaagtttt
    10261 acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat ttggatgaat
    10321 ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact cccaaccttg
    10381 ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg gtagcggcga
    10441 caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca gtgggatccg
    10501 ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat cagcagccga
    10561 gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga gtaactcttc
    10621 agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataaggacag aatcaacgct
    10681 tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt taccggaggc
    10741 cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat cactcatctt
    10801 tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc ggcggcgttt
    10861 tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc tgtgccattg
    10921 accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata ttgccaggtt
    10981 cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt tgtaaatttt
    11041 gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct gactggaaat
    11101 caatacccaa ttccgctagg gcagactgcg ctttttctaa tgccgatttt tcttcgccag
    11161 aaacttttag ccgcaacgga atcagcagcg gctgggcgca aacgggcgct tcacccggcg
    11221 tcaattgtgc ctgacgcagc caacgttctg ccactggcaa ggataaaagt gaaatgttgc
    11281 catcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga ccaaaactct
    11341 gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttggtttt tgcatcggcg
    11401 cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag cctggctgcg
    11461 cattcggcca gggggcagcc ggacgactgc ctgaggcggg cgcaggagag tagcgcggag
    11521 ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc acgcggtttt
    11581 ccgggatggc acgcggtgcg ggttgtggtt catcgtccag cggtagcggc gtttccagtt
    11641 gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga cgcgactgat
    11701 ggaaacgcac ttcgtgtttg gcggggtgta cgttgacgtc cacctgatgc gggtcgatct
    11761 ccagatacaa cacaaatgcc ggttgctgat cggcccccag tttgtcttcg caggcctggc
    11821 ggatcgcatg attgatcagg cgatcgcgca tcatgcgacc attcacgtag caatactgaa
    11881 tttccgccag tgcgggcgta gtgtgatttg gatcggccac ccagccgcgc agggtgagat
    11941 cgccgtgttg ccattcaatc gccagcgctt gttcgagaaa agcggtgccg caaatcgcgc
    12001 ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc acaattttac
    12061 cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg cggatgattt
    12121 catcaatatg gctaaattcg gttttctcgg tgcgcaggaa tttgcgccgc gccggggtgt
    12181 tgtagaacag atccagcacc tccagcgtcg ttcccaccgg atgcgctgcc ggtttaaccg
    12241 tcacgtccat gtcgcgtcct tcggcatagg cctgccaggc ttcctgctgt tctgcggtgc
    12301 gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca ccgcgaaagc
    12361 ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg gcatgacgag
    12421 ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga atgcggataa
    12481 gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca tcgaggctgt
    12541 tttccactag ttctttgact accgacgcag gtcgctcgac cacctcacct gcggcaatct
    12601 ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag tttggcagcg
    12661 tgcgatctgg cgtcgtcacc gtactggcag tttgtgccgt tgcaccctgc ggcgcagatt
    12721 gcatcggatg cgcaaggaag taattgcgca ggcctttgta aatggcttct gccagctgtt
    12781 gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata aaaccggttt
    12841 cgaccagtac tgatgggata tccggcgagc gcagaacgcc aaggctggcg tgttctggtc
    12901 gacgtttatg tatttcgcca atgcgttgca actgactgat catactggtc gctacatcat
    12961 accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt aaataggggt
    13021 cagactgact gttcgccagc acatcacccg ccccacccag cagctccgac agtttctcgt
    13081 gctgctccag ccagctggcc atttcactgt tggcgcgacg gttagagagc acccatacgg
    13141 aagcgccagt cgcactacgg ttcggtgcgg catcagcgtg aatcgacacg aggaaattgg
    13201 cgttttgctt atgtgccaca tcactgcgcc ccatcaccga gataaagtaa tccccgtcac
    13261 gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat ttacgcgcga
    13321 tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca gggtcctgac
    13381 cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc gtcgcgcgcg
    13441 ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta ctttccgttt
    13501 taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc gtttcaacgc
    13561 gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta atcgtaaaga
    13621 cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttaccg ttttcggtaa
    13681 gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga atcgccttca
    13741 ccagattatt gccgctgaac aacaacggca gtccctgaat catgcccgtt tgtttgatat
    13801 cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg ccaataaaac
    13861 tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag agcgtcgcgg
    13921 cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg atgcgataca
    13981 tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat tcacccgcag
    14041 aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg tgtatttcga
    14101 catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg cagatggcat
    14161 cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg gcaaggcggt
    14221 acaaatcaaa gtgatagacc attaagttgt cgagcgtata gggttcgacc agcgtataag
    14281 tggggctttt gacattaccc tgatgaccca gagcctgtaa aaagccccgg ctaaaggtgg
    14341 ttttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg caggctttcg
    14401 ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt actcgattca
    14461 tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga aaagagatcg
    14521 gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc cgcaccgtgc
    14581 gcgacacagc ctgcacaggc tgcatcatac ggcgacagtt tttgcccaag caatgcgcca
    14641 ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt tccggcatca
    14701 ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt cagcaccgct
    14761 acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact ttcaatttca
    14821 gcgacggaac agcctaacaa ccgtgcggcc tcgctaggat gcggcgtgat cacgcgattg
    14881 tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca caacatcggt
    14941 ttgcgaaaat tctcaacttt ttgcagggct tttttccccc actcttgctg gcccagaccg
    15001 ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc catagtcagt
    15061 tcatgcacca tcaattctgg tcgtgcagtc agcagcggcg caatgttctc actgcgggtc
    15121 agtactcgga ctaaaccagc accagcacgt agcgccgctt cccccgtcat acgaatagcc
    15181 cccgccgtgc cgtgatcgcc accgataatc accagccgcc cgtgatcgcc tttatgcgaa
    15241 gtcgggcgac gcggtttcag ccagtgagaa agttgttctg ccgaaaaccg ctgaattttc
    15301 gtctcctgac ctgccagcca gctatccagc cccagtgagt caaaatgcag ttgtcctgta
    15361 acatcccgcg cttttccagt gagcaagcct agtttcagcg caataaaagt gatggtgtga
    15421 tcggcgttga tcactgcgcc tggcgtagcg ccggtttcag ccagcaggcc ggaagggata
    15481 tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg gctaatggat
    15541 tcgcggggcg cttgctgcaa gccggtaccg agcagcgcat caacaatcag atctaccgat
    15601 tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca tgcttcgcgt
    15661 gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggccaacaa cgtgacctca
    15721 atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt attaccatga
    15781 ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac ctggaatgcg
    15841 gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc tgccgcctcg
    15901 cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact tacggggttt
    15961 ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt agcgcaaaaa
    16021 attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga taccgatctc
    16081 agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg cgaaatggac
    16141 tggatggcac gtcacggtat gctgcgcgct cgcccccatg agttattacc cggtacgctg
    16201 cgtgtaatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc cagcacgctg
    16261 aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta tcacaaactt
    16321 ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt ttcgctgaat
    16381 tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga aaaagctggg
    16441 ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc gttcttcttt
    16501 ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga ggaaggatgc
    16561 ggcaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga gccatatacc
    16621 gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc gctcccggaa
    16681 gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca gcttatctgc
    16741 ccgtggaatc gctattcgca actcactaca gaagacgatt tcagcccgcg taagccgcta
    16801 cacgcaccgg aactcattga gttatttgcc tggagcgaag agaagttttt aaaagtcacg
    16861 gaaggatcgg cgattcgccg tataggtcac ctgcgctggc tgcgtaatat cgccgtggca
    16921 ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg taaaggtgag
    16981 cacccacttc tcgatgagca catagcgtgg gcgattgcgc agcaaataga gagacgaaat
    17041 gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt gattgaaaaa
    17101 gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa aacgcattgc
    17161 aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc ttgattttta
    17221 acttatttat caataggtta aaaaaatacc attcaccgtg cgaagtttct ttgaaattaa
    17281 gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag acaacaaaaa
    17341 ccagcaataa cgcatcgcgt cgctgtagat attttattga gagaagaatt tggagcggga
    17401 aacgagactc gaactcgcga ccccgacctt ggcaaggtcg tgctctacca actgagctat
    17461 tcccgc
//
LOCUS       DAJTRD010000082        17328 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTRD010000082 DAJTRD010000000
VERSION     DAJTRD010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17328
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.082"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OO315_002994"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OO315_002994"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5891821.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OO315_002995"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OO315_002995"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5891822.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OO315_002996"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OO315_002996"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5891823.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OO315_002997"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OO315_002997"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5891824.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4817
                     /gene="citX"
                     /locus_tag="OO315_002998"
                     /pseudo
     CDS             4267..4817
                     /gene="citX"
                     /locus_tag="OO315_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4792..5670
                     /gene="citG"
                     /locus_tag="OO315_002999"
     CDS             4792..5670
                     /gene="citG"
                     /locus_tag="OO315_002999"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5891825.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5721..7183
                     /gene="citT"
                     /locus_tag="OO315_003000"
                     /pseudo
     CDS             5721..7183
                     /gene="citT"
                     /locus_tag="OO315_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7297..8103
                     /gene="rna"
                     /locus_tag="OO315_003001"
     CDS             7297..8103
                     /gene="rna"
                     /locus_tag="OO315_003001"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5891826.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8332..8742
                     /gene="rnk"
                     /locus_tag="OO315_003002"
     CDS             8332..8742
                     /gene="rnk"
                     /locus_tag="OO315_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5891827.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8973..10211)
                     /locus_tag="OO315_003003"
     CDS             complement(8973..10211)
                     /locus_tag="OO315_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR5891828.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10432..10860
                     /gene="uspG"
                     /locus_tag="OO315_003004"
     CDS             10432..10860
                     /gene="uspG"
                     /locus_tag="OO315_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5891829.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="OO315_003005"
     CDS             complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="OO315_003005"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5891830.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="OO315_003006"
     CDS             complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="OO315_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5891831.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13726..14472
                     /gene="dsbG"
                     /locus_tag="OO315_003007"
     CDS             13726..14472
                     /gene="dsbG"
                     /locus_tag="OO315_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5891832.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14682..15584
                     /locus_tag="OO315_003008"
     CDS             14682..15584
                     /locus_tag="OO315_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5891833.1"
                     /translation="MANLYDLKKLDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15731..15934
                     /locus_tag="OO315_003009"
                     /pseudo
     CDS             15731..15934
                     /locus_tag="OO315_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(15953..17100)
                     /locus_tag="OO315_003010"
     CDS             complement(join(15953..16863,16863..17100))
                     /locus_tag="OO315_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5891834.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDIFKKSRGVLRKAVRLRYAFIRDNS
                     CCWPVRLLCRVLDVHPSGFYAWLQQPHSQRHQADLRLTGQIKQFWLESGCVYGYRKIH
                     LDLRDSGQQCGVNRVWRLMKRVGIKAQVGYRSPRARKGEASIVSPNRLQRQFNPDAPD
                     ERWVTDITYIRTHEGWLYLAVVVDLFSRKIIGWSMQSRMTKDIVLNALLMAVWRRNPE
                     KQVLVHSDQGSQYTSHEWQSFLKSHGLEGSMSRRGNCHDNAVAESFFQLLKRERIKKK
                     IYGTREEARSDIFDYIEMFYNSKRRHGSSEQMSPTEYENQYYQRLGSV"
     gene            17158..17328
                     /locus_tag="OO315_003011"
                     /pseudo
     CDS             17158..17328
                     /locus_tag="OO315_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         4274 a         4494 c         4184 g         4376 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcgaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agacccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgcgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa
     4741 aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg
     4801 cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg
     4861 ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat
     4921 cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg
     4981 gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca
     5041 ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc
     5101 cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt
     5161 gcggcaattg gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct
     5221 acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga
     5281 ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc
     5341 gaagcaggtt atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat
     5401 caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac
     5461 ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag
     5521 gcgcaaacat tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg
     5581 cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg
     5641 atccttacct gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata
     5701 ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc
     5761 cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg
     5821 catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc
     5881 cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc
     5941 tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat
     6001 ggggcctggc tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg
     6061 cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca
     6121 tggacaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac
     6181 cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc
     6241 tgccgccgtt gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt
     6301 tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg
     6361 accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt
     6421 gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct
     6481 ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg
     6541 tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt
     6601 attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg
     6661 tctgctggca gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg
     6721 ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg
     6781 cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg
     6841 attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct
     6901 gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa
     6961 aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat
     7021 catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta
     7081 tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat
     7141 gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct
     7201 gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag
     7261 atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc
     7321 gcgttgctcg cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca
     7381 aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc
     7441 cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg
     7501 accaacaaaa ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt
     7561 gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc
     7621 ccgaatctac cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca
     7681 ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg
     7741 gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc
     7801 ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg
     7861 gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg
     7921 ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact
     7981 gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc
     8041 ttgccacaac ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat
     8101 taatcctctg atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt
     8161 atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg
     8221 agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct
     8281 tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga
     8341 ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc
     8401 gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg
     8461 tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc
     8521 aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat
     8581 agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt
     8641 ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa
     8701 ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga
     8761 taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc
     8821 ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca
     8881 tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg
     8941 ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc
     9001 accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc
     9061 accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca
     9121 aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt
     9181 aataattctc ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta
     9241 tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta
     9301 acaatgccgc cacgcctgac cgccgcaata cactggcgca acgctttacc gctgctgccc
     9361 tccagtttca ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc aaaaccgacg
     9421 gcgtctatta ctgcatccac gccccggtga cctgccgttt gttcaataat tgactgtgcc
     9481 ggatcgctat cttcatcgaa attaatcggg atcgcgccgt agcggtcagc ggcgaaacgc
     9541 aagcggtagg gatgatgatc aacaacaaaa atctgttccg caccgagcaa ccgtgcacag
     9601 gcgattgtca acaatcccac aggaccagca ccatagactg caacgcttga accttgttgg
     9661 atctgcacat tttttgctgc ctgccatgcc gttggcagaa tatcagaaag gaaaagggct
     9721 ttatcatctg aaagcaaagg cggtacttta aacggcccca cattcccttt cgggacgcgg
     9781 acatattccg cctgcccgcc aggaacgcca ccatacaggt gactataacc aaacaatgcc
     9841 gcgggagcag gtatctgttt tttattgagc gcagcgcctt tacccgcatt ggtattttcg
     9901 caggcggcat attgctgtaa tcgacagaaa aaacagtcgc cacaagcaat gacgaacgga
     9961 attaccaccc ggtcgccttt ttgcaaattt tttacgtcct tcccggtttc aacgacttcc
    10021 cccataaatt catgaccaaa aatatcgcca tgtttaacct ggggtatttt gcctcgataa
    10081 agatggaggt cagagccaca gatcgccgtt accgtaatac gcagaataat atcatctgcc
    10141 tgttcaatgc ccggatcggg aacattttct acctgaacgt gatgtgggcc gtgataagtc
    10201 aatgctttca tgcaacctcc agagacagtt caaaaaagag tcagtcttta agggtagcta
    10261 tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga attcctagtg ttcagtgaga
    10321 atatattaat cacagaaacc gttcgtaagg aaaaagaacc agacaaatat cagggataaa
    10381 tattataatc atggagaatg atttgttcat gattaacagg gagaaagggt tatgtataag
    10441 gcaatcatta tgccagttga tgtatttgaa atggaattga gcgacaaagc tgttcgccac
    10501 gctgaattcc tcgcccagga tgacggagtt attcatctac ttcacgtact acccgggtca
    10561 gccagcctga gcctgcaccg ttttgccgct gatgtgcgtc gttttgaaga gcatctgcaa
    10621 catgaagcag aagaacgtct gcaaacgatg gtcagccact tcaccatcga tccttcccgc
    10681 attaaacaac atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt ggcagaagaa
    10741 ctgggggctg atgttgtagt tatcggttct cgcaacccat cgatttcgac ccatctgtta
    10801 ggttctaacg cctcgagcgt gatccgccac gccaatctgc ctgtactggt cgttcgctaa
    10861 cagataaaag tatttatgcc gccctgcctg gaacatcccg gcagggcttt ttaatgccat
    10921 aaaaaagccg ccaggtttga ccccggcggc taagcaattg caggtgaatc ttacttcttc
    10981 ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt tggcaccttc
    11041 gccagtggcg atgatgatct gcttgtacgg aaccgtcgta cagtcacccg ctgcgaacac
    11101 gcctttgacg ttggtttcac atttcgcatc aatgataatc tcgcccatgc ggttacgttc
    11161 gactgcgcct tccagccagt tggtgttcgg cagcagacca atctggacaa aaataccggc
    11221 cagttcgatg ttgtgaatat tgccgctgac acgatctcga tattccagac caacgacttt
    11281 gctgccgtcg cctttcactt ccgtggtttg cgcattcaga ataatgtcga cgtttttcag
    11341 gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa attccagcag
    11401 cgttacgtgc tcaacgatac ccgccaggtc gattgccgct tccacgccgg agttaccgcc
    11461 gccgataacc gctacgcgtt tgcctttaaa cagcgggccg tcgcagtgcg ggcagtaggt
    11521 cacgccttta gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc attttgcacc
    11581 cgtcgccacg ataatgctgc gtgctttcag taccgcgcca gaagctgttt caatctggtg
    11641 tagaccacct tcaaccgctg ccgggatcag tttgctggcg ctctggctgt cgatcacatc
    11701 aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc aacttctgcc cttcagtctt
    11761 cggtacagaa atgtagtttt cgatatcaac ggtatcgagg atctgaccac caaaacgttc
    11821 gcccatcaga ccggtacgga tgcctttacg tgcggagtaa attgctgccg ctgcacccgc
    11881 cgggccggaa ccaacgatta atacgtcata agcatcacgc ttgttcagct cttctgccgc
    11941 acgattttcc gcgccagtat caattttggc aacgatttca gtcaacgtca tgcggccctg
    12001 accaaactct ttcccgttta cgaacactgc cggaacgccc atcacgttgc gatcggtgat
    12061 ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt tcaatacgct
    12121 catcaggttc agcgcctgca ccacgtccgg gcagttatgg caagagagcg agtaataggt
    12181 ttcgaattca aaatcaccgt caatatggcg aatctgctcc agcagagact gcgcttcttt
    12241 cgacggatga ccaccggtcc acagcaacgc cagtaccagc gaggtgaact cgtggcccag
    12301 cggggagcct gcaaaacgtg gtccctggtt ggaacctggg ttggtgatca ggaaagacgg
    12361 cttacgcact ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca gttctgcgat
    12421 ttcagccaac agttccttga tttctgccga tttagcgctg tcatccagcg tggcaattaa
    12481 ctcaacaggc ttggtcaatt tctcaaggta agccttgagt tgagttttca tatttgtgtc
    12541 gagcatgttt atctcctggg ctttaaacat catcatgcaa gcggcctctg cggctacctg
    12601 gatgcagctt gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc cgaagaatta
    12661 ggtgaatttc cgaagtattt cgcgtcagag caaggcgact agcctgtgaa tccccgggag
    12721 cttacacaag taagtgaccg gggtgagcgg gcgacgccaa cgccgctatg gcgtgaaaga
    12781 cgaaggaaat ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt
    12841 ctttccattt agccgggcaa acttcacctg ggtgagaagc tacgtactga gctgctttga
    12901 ttttacgcag cagatcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct
    12961 ggatgatacc ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac
    13021 gcatgttgtc gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga
    13081 ttttagcgat ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag
    13141 atactgcgta tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac
    13201 ccagttcggt cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc
    13261 ggccttcggt atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt
    13321 taaaaggttt aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga
    13381 gatgtaaggt aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa
    13441 gttttacctt acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa
    13501 aaagtaaaat ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc
    13561 agcgcctgcc tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca tggattacaa
    13621 caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt
    13681 tatgcctttt actcgacttt tgcactgact gaaaagaaca aattaatgtt aaaaaagata
    13741 cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttccttc cccagtaaaa
    13801 gcgattgaaa aacagggcat tacaatcatc aaaacgttcg atgcccccgg aggaatgaaa
    13861 ggttatctcg gaaagtatca ggatatgggc gtcaccatct acctgactcc cgatggtaag
    13921 cacgctatct ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc
    13981 gaaaaagaaa tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac
    14041 tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtcttctc cgatccgttc
    14101 tgcccatatt gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc tggcaaagtg
    14161 caattaagaa cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca
    14221 attcttgcct ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc tggttgcaag
    14281 cttaagctaa acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt aagtgacaat
    14341 gagaaactga tggacgatct gggggcaaat gtcacgccgg ctatctatta catgagtaag
    14401 gaaaatacgc tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg
    14461 ggaaataaat aaaaggtaat tcatggatgg ttgaactata tccctgcttt tcaaccatcc
    14521 taaaaaagca tgaatttctc gctctaccct aagtggctgt gctattttag aaaaacttaa
    14581 tcctgagtta agcaaaaaaa cataatcaat aaaaatacat acgcaaaaaa cattgattaa
    14641 gtgaatatac catggaagaa aaatataaac cggagtagtg tatggccaat ctctacgact
    14701 tgaaaaagct cgacttaaat cttcttgtca tatttgagtg tatttaccag catttaagca
    14761 ttagcaaagc tgctgaatca ctatatatca ccccatcagc cgtgagccag tcgttacaac
    14821 gcttacgtgc gcaatttaat gaccctttat tcattcgctc aggaaaaggt atcgcgccga
    14881 cgacaacagg gttgaatctg catcaccatc ttgagaaaaa cctcaaaggc cttgaacaaa
    14941 caattaatat agtcaataag tccgaactaa agaaaaactt tattatctac ggccctcagc
    15001 tcatttcttg ctctaataat agtatgctga tccgctgcct acggcaagat gcctctgtcg
    15061 agattgagtg ccatgatatc ctcatgtcag cagagaatgc tgaagaactg ttggttcacc
    15121 gcaaagccga tctggtgatc acccaaatgc cagtgatcag ccgttccgtc atttgcatgc
    15181 cattgcatac aattcgcaat actctaattt gtagtaataa gcatccgcga attactgaca
    15241 atagcactta tgaacaaatt atggcagaag agttcaccca attaatagcc aaatcagcag
    15301 gagttgatga tattcaaatg gaaatagacg agaagtttat gaatcgcaaa atttcatttc
    15361 gtggttcctc tttgctgaca ataattaata gcattgcagt tacagattta ctgggaatag
    15421 tcccctatga actttataac taccaccgtg actttcttaa tttaaaagag ataaaactag
    15481 aacatccgtt accttcaata aagctttaca tttcctataa taaatcttct ttaaataatt
    15541 tggtgttctc ccgatttatt gaccgtctaa atgagagttt ttgatatata actatagaca
    15601 gctactataa ctttcatcta tttattcaca gcgcgaatga aaaataaaac actctgcttt
    15661 acagtgttta ctttataatt aacagtctta tctcactgtt caccctgtaa cgttcagaga
    15721 gcgtcttcag atgtctattt ataaaattcc tcttccgctc aatattctgg aagctgcacg
    15781 agagcgtatt acctagacac tcaacaccct accccgcgtt tgtgtctcat tttctggcgg
    15841 caaagactct ggtttaatgc tgcatctgac agccgaactt gcccgacaaa tgggcaaaaa
    15901 aatctgcgtt ttgtttatca actgggaggc gcaatgaatc gccacggata atctagacac
    15961 ttccgagccg ttgataatac tggttttcat attctgtcgg tgacatctgt tcgctagaac
    16021 catgccgacg cttactgtta taaaacattt cgatgtaatc aaaaatatca ctgcgggctt
    16081 cttcccgcgt tccgtagatc tttttcttta tccgttcacg tttcaacaac tggaaaaaac
    16141 tttctgcaac cgcattatca tggcagttac cgcgacggct catgctaccc tccaggccgt
    16201 gtgatttcag gaacgactgc cactcatggc ttgtgtactg actgccctga tccgaatgaa
    16261 ccagcacctg tttttcggga ttacgccgcc atacagccat cagcagtgcg ttcaggacaa
    16321 tgtcctttgt catccgggat tgcatggacc agccgataat tttgcgtgag aacagatcaa
    16381 caacaacggc aagatacagc cagccttcgt gggtcctgat gtaggttatg tccgttaccc
    16441 aacgctcatc aggagcatcc ggattgaact gtcgctggag cctgttgggt gacacgatac
    16501 tggcctcgcc tttacgtgcc cgcgggcttc ggtatccgac ctgagccttt attccgacac
    16561 gtttcatcag tctccagact ctgtttactc cgcactgttg cccgctgtca cgcagatcca
    16621 gatggatttt gcgataacca tagacgcatc ccgattccag ccagaactgt ttaatctgtc
    16681 ctgtcagtct caggtctgcc tgatggcgtt gtgaatgcgg ctgctgaagc caggcgtaaa
    16741 aaccactggg atgaacatcc agcacccgac agagcaggcg aacaggccag caacaggagt
    16801 tgtcacggat aaaggcgtac ctcagtcgga cagctttgcg aagtacgccg cggctttttt
    16861 taatatgtcc cgttcgtcgg taacccgttt cagctctttc tggagacggc ggatctcggc
    16921 ctgagcatct gactgttctt tattagtgga agaatccgga ccgtacttct ttatccaggc
    16981 ataaaggctg tgggtggtga tatcgagacg tgttgcaacg ctggcaacag aataaccgcg
    17041 atcaacaacc tgtttgactg cttcagtttt aaactcttcg ggataacgct taccgctcat
    17101 gggcacctct ctttaagcca tcttaaatga ctctgaggtg tctgttaaac ccgtggcgat
    17161 tcacaattct cttgcaccat taactatgtt cagtccctgc gcgagttgta caccgatgtc
    17221 atcgaagagt tttactgggt tgcgctcccg cttacgacgc aaaattccct ttcacaatac
    17281 caacccgaat ggcagtgctg ggaacctgat gtcgaatggg tgcgtcag
//
LOCUS       DAJTRD010000083        17090 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTRD010000083 DAJTRD010000000
VERSION     DAJTRD010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17090
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.083"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..508
                     /locus_tag="OO315_003012"
     CDS             <1..508
                     /locus_tag="OO315_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR5891835.1"
                     /translation="FREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGF
                     TQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIR
                     HNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQ
                     YSRAWTVK"
     gene            complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="OO315_003013"
     CDS             complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="OO315_003013"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR5891836.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1778..2866
                     /gene="hcxA"
                     /locus_tag="OO315_003014"
     CDS             1778..2866
                     /gene="hcxA"
                     /locus_tag="OO315_003014"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5891837.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2876..3073)
                     /locus_tag="OO315_003015"
     CDS             complement(2876..3073)
                     /locus_tag="OO315_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5891838.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="OO315_003016"
     CDS             complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="OO315_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5891839.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5541..5954)
                     /gene="entH"
                     /locus_tag="OO315_003017"
     CDS             complement(5541..5954)
                     /gene="entH"
                     /locus_tag="OO315_003017"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5891840.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5957..6703)
                     /gene="entA"
                     /locus_tag="OO315_003018"
     CDS             complement(5957..6703)
                     /gene="entA"
                     /locus_tag="OO315_003018"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5891841.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6703..7560)
                     /gene="entB"
                     /locus_tag="OO315_003019"
     CDS             complement(6703..7560)
                     /gene="entB"
                     /locus_tag="OO315_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5891842.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7574..9184)
                     /gene="entE"
                     /locus_tag="OO315_003020"
     CDS             complement(7574..9184)
                     /gene="entE"
                     /locus_tag="OO315_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5891843.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9194..10369)
                     /gene="entC"
                     /locus_tag="OO315_003021"
                     /pseudo
     CDS             complement(9194..10369)
                     /gene="entC"
                     /locus_tag="OO315_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10661..11617
                     /gene="fepB"
                     /locus_tag="OO315_003022"
     CDS             10661..11617
                     /gene="fepB"
                     /locus_tag="OO315_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5891844.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11621..12853)
                     /gene="entS"
                     /locus_tag="OO315_003023"
     CDS             complement(11621..12853)
                     /gene="entS"
                     /locus_tag="OO315_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5891845.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGAAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12964..13968
                     /gene="fepD"
                     /locus_tag="OO315_003024"
     CDS             12964..13968
                     /gene="fepD"
                     /locus_tag="OO315_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5891846.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13965..14957
                     /gene="fepG"
                     /locus_tag="OO315_003025"
     CDS             13965..14957
                     /gene="fepG"
                     /locus_tag="OO315_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5891847.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14954..15769
                     /gene="fepC"
                     /locus_tag="OO315_003026"
     CDS             14954..15769
                     /gene="fepC"
                     /locus_tag="OO315_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5891848.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15766..16899)
                     /gene="fepE"
                     /locus_tag="OO315_003027"
                     /pseudo
     CDS             complement(15766..16899)
                     /gene="fepE"
                     /locus_tag="OO315_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3993 a         4863 c         4445 g         3789 t
ORIGIN      
        1 ctttcgtgaa aagccagtgg attgcgttct gtgggttaaa aacagccaac ttatgcctaa
       61 tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgcaaa aatccattga
      121 aatagatggt tttacccaac ccattgtggt cacacatacg gataaaaatg ccatggaaat
      181 cgtggatggt tttcaccgcc atgaaattgg taaaggctca tcatcattaa aattacgctt
      241 aaaaggttat ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac
      301 tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg
      361 tgaacttagc cagttgggat gggatgataa taaaattggc aaagagctgg gaatggacag
      421 cgacgaagtg ctgcgactga agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca
      481 atattcccgc gcctggacgg ttaaatagct aaagctggcg caggcgttca gctgctgcca
      541 gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag tttatgtggg aagggatcgg
      601 cgcaaaacac cgacagcgga atcgccgcta cgccgtgctc ctgcgtcagc cactggcaaa
      661 actcaacatc atccagggta gaaaccgcgc tgtaatccac cagcaaaaag tatgtacctt
      721 cacacggtaa aatctccagc cggctttcat ttaaagcatt caccagaata tcgcgcttct
      781 ggcgataaaa gtccggtaac gcaagataat gctcaggttc tgcacgtagc atatcagcaa
      841 gcgccagctg tgccggggta ttcaccgaaa aggtcagata ctgatgtacc ttgcgaattt
      901 cggcgctgat gggcgctggc gcaacacaat aacccacttt ccagccggtc atatgatagg
      961 tcttgccaaa tgaagaaacc gccactgccc gctcacgcag ctgcggatgc gccagcacac
     1021 tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta atgacaaaaa
     1081 tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc cagacagttg
     1141 cactggggtt atgcggagtg ttgaggatca ccagtctggt gcgctcgctt aataatgcgg
     1201 caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgt ttcactattc
     1261 ccccagaaag cgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa caaatcactt
     1321 catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc gcccctgccg
     1381 ttacggtgat atcgctatcg gcatctggtt gatagccata caaacgttcc gttttctgag
     1441 caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt gccccctggg
     1501 caacgtggta cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt
     1561 gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg aaaatagtgg
     1621 tgccaagttg tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac
     1681 ctgacgaagt tgcgtgttga aggcactata acacgatgcc agtatttggc aatcaagacg
     1741 tttagatgtc taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt
     1801 ggtcgtcggc ccggctaact acttttcaca tccaggaagt ttcaatcacc tgcacaattt
     1861 tttcactgat gaacaacttt ctcgcgcggt gtggatctac ggcaaacgcg ccattgctgc
     1921 ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg gcaaagcata ttttgtttcg
     1981 tggtcattgc agcgaaagcg atgtacagca actggcggct gagtccggtg acgatcgcag
     2041 cgtggtgatt ggcgtcggcg gcggcgcact gctcgacacc gcgaaagccc tcgcccgccg
     2101 tctcggtctg ccatttgttg ccgctccgac gatcgccgcc acctgcgcag cctggacacc
     2161 gctctccgtc tggtataacg atgccggaca ggcgctgcat tatgagattt tcgacgacgc
     2221 caattttatg gtgctggtgg aaccggagat tatcctcaat gcaccgcaac aatatctgct
     2281 ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc
     2341 agaaacgttg ccgctaaccg tgcggctggg gatcaataat gcgcaggcca ttcgcgacgt
     2401 cttgttaaac agtagcgaac aggcacttgc cgatcagcaa aatcagcagt taacgcaatc
     2461 attttgcgat gtggtggatg ctattattgc tggtggtggg atggtcggtg gtctgggcga
     2521 tcgttttacg cgtgtggcgg cggctcatgc cgtgcataac ggtctgactg tcctgccgca
     2581 aaccgagaag tttcttcacg gcaccaaagt cgcttacgga attctggtgc aaagcgcctt
     2641 gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt ttcatctgcc
     2701 gactacgctg gcggagctgg aggtggatat caataatcag gcggagatcg acaaagtgat
     2761 tgcccacacc ttgcgcccgg tggagtccat tcattacctg ccagtcacgc tgacaccaga
     2821 tacgttgcgt gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg caaatttagc
     2881 agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg aaaaactcct
     2941 cgtaggtcat cggcgtttga tcgggatgat taacccgcat atgttcgaca tagttgtcgt
     3001 aatcaggcat accaatcatc agcttcgccg cctgacctaa atattttccg gcttttgcca
     3061 gtgaatcaaa catcttggcc tccgctaacc atgttgctga aagagtgccg gatgcggcgt
     3121 gaacgcttta tccggcctac aaatgctatc aacgcactcg gtcagtcccc tcgccccatc
     3181 ggggagaggg ttagggtgag ggggaaccct cggcactatg ccaacattcg ccaacatccc
     3241 ccctcactct gactttagtg cgcgcctttc gcctgcgcta cgatctcctc gacattttcc
     3301 ggcatcggct catacggcgt ttctttcgcc gttggcttcg gatctttcaa tgccgcaagt
     3361 gccgtcttaa tcgagaacag tgccaggacc accacgacca ccataaagaa gatggttaac
     3421 ccggcatcca gacggttgtt gaacaccagt tgcgccagct gtgactcagt atactgcgac
     3481 ggaatattgc cgctgtcgat cattgcctgg aacttgttag caatgaccag gaagccgact
     3541 ttcgcatccg ggctaaacgc tttcagccag tcagcggtca gggtacaaat cagcagccag
     3601 gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg
     3661 gcacagagca tcagcgccat ccctgccagc atctggttgg caataccaaa cagcggccac
     3721 agagtgttaa taccgcccag cggatcgacc acgctctgat ggaggaagta gccccacgcc
     3781 agtacgcaca gcgctgttgc cagcaggtta gcaggcagtg aatcggtccg cttcaggcca
     3841 ggagacacca cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt acccgcatct
     3901 accgccgtca gaataaacag agcttcaaac agaatggcga agtgatacca gaacgccaca
     3961 tccatcatac cacccagcgc accgtgcagg atgtaagcca tccccaccgc caacgtcggc
     4021 gcaccgcccg cacgggaaat gatcgactgt tcacccactt cgctggcaat ctggttgagc
     4081 gtatctggtg taatgctaaa gccccagcta ctcaccacct gcgcggcaga agcgactaca
     4141 tccgccgtcc ctgccggagc cagcaccgcc attgggctgt tcatggcaaa atacacgccc
     4201 ggatcgatga tacaggcaga aactagcgcc ataatcgcca cgaaggattc catcaacatc
     4261 ccaccgtagc cgataaagca cgcctgccct tcgttcgcca gcatcttcgg cgtggtgccg
     4321 gaagagatca gcgcatggaa gccagacacc gcaccacagg cgatggtgat aaacaggaac
     4381 gggaacaggt taccggtcca taccgggcca gtgccatcga caaatttggt cagcgcaggc
     4441 atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca gaccgacgat agtcccgatt
     4501 ttcaggaagg tcgagagata gtcacgcggg gccagcagca accacaccgg cagcaccgcc
     4561 gccacaaaac cgtagcccac cagcatccag gtcagctgca cgccggtaaa gtcaaagtac
     4621 ggtgcccagg tcggactttc tgccacccag ccgccagaga taatggcgaa aatcaggaac
     4681 accaggccaa tcactgacac ttcgccaatg cggcccggac gcagatagcg caggtagatt
     4741 cccataaaaa tcgccagcgg aatggtgaac gcaacggtat acgttcccca cgggctatga
     4801 gtcagggctt tcaccacgat catcgccagt actgcaagga taatgaccat gatcataaag
     4861 caggctacca gcgcaatcac cccggcagtt ggccccatct cttctttgac cagctcaccc
     4921 agcgagcgac cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg caccgcaccg
     4981 gcgagaacca ccccggcgag cagccagatc atccccggca ggtagcccat ttgcgctgcc
     5041 agcaccggcc ccaccagcgg ccctgctccg gcaatggccg caaaatggtg accgaacagc
     5101 actttcttgt ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc tggcgtcata
     5161 cgcgtcgggt caaccgccag cacatttttg gcgatataca gcccataaaa acggtaagcg
     5221 atcagataga tacagaccga cgccaccaca atccacagcg cgttgatctg ttccccacga
     5281 tttaaagcaa tgtatcccag agcaaatgct cccattacag agagcactgt ccagacgagg
     5341 tatttccctg atttgttcat agttgttatc cgtgtgcgtg tccatagaga tgttacattt
     5401 tgtttctata acatctttgt acgatttaac aacacggtaa ccatgtaaat cggagttgaa
     5461 accaatattt aacttaacaa tgttaactcg atcactccgt taaccgagtt ttcctgcaat
     5521 ctcatttaat tctgtcgggc tcatcccaaa actgccgtac ccagccgaca agtgcagcaa
     5581 cgccgcccct gttcatcgaa aacgacgatt tcccaactct gattttgccg cccaagatgc
     5641 agcggctggc agacgccgcg taccttgcct tcagacaccg ggcgatgatg ggtggcgtta
     5701 agttccgtgc ccaccacaca ctgcccgtca cgggtcatca taaatccggc catcgatccc
     5761 agcgtttcag ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg ctgatgagta
     5821 cgggtatcaa ccggcatttc ggcttccagc acatcatctc ccagaagggt atacacaatt
     5881 cccagatgcg ccaccattgt gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg
     5941 cgtttccaga tcatgcttat gcccccagcg ttgagccgcc atcgaccaca atatcctgta
     6001 gggtgatatg gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg
     6061 gacgggcgat tttccccagc ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac
     6121 gctgttcttc ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg tcggtggagc
     6181 caggcgaaac cacattacag cgcacgccgc tacccgccag ttccagcccg acgctcaacg
     6241 ccaggctttt cagcgccgct ttcgatgcgc cataagcact cataccaata cgcgccgtgt
     6301 gtgcggcgtc ggacgccaca gtgacaatcg ccccgccccg ctgacggcga aactggttca
     6361 tggtttgctg gaacaggtta aacgcaccgc cgacgttaac cgcaaaagtc tgctgccagt
     6421 cctctttact gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca ttgatcagca
     6481 cgtccagtcg ctccgtttca gctaacagtc gctgacatac ctgcgccacc tgcccggcgt
     6541 cggcaacatc cattacttcg gtcgcaaagg gatattgctc ctgagtgaac gcttgatcaa
     6601 aacctgtaac tttcgctccc gcctcaacaa acgtcagcgc cgtggcgtag ccgatacctt
     6661 tacctgcgcc ggttacccag acatttttac cgctgaaatc cattatttca cctcgcggga
     6721 gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa agtcgatatc
     6781 accatgcact ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat ccagaccgta
     6841 gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg tccagcaacg gcaggatcac
     6901 ctcacgcagc gccgctttgc tggcggggat aggtgctggc agtaattctt cggtcatcac
     6961 cacccggcca gaacgtccgg ccacatattt cagcgacatc aaatgctcgt cacggctgaa
     7021 atcagccagc gcatccgcca ccataaacgg tttaatatcg cgcataaatg cgtcggttgc
     7081 ggtggtcata cagccaatgt gggcatatac tcctgtaata atcagctggt tacgtccact
     7141 ctctttgagc atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag
     7201 caccgtgtcg tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt ccggcgagcg
     7261 ggtcaggccc ggcccccaca tatcattcaa cagcgcccga tcttcatcgc tctgctcttt
     7321 cggctgggcg gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc gcagcgcagc
     7381 aatattcgcg atcacctgct ccatcatcgg gcagttctcg ccccagaagc tgacaaaata
     7441 gtcctgcata tcatggatta acaacgcggc acgttgcggt tcaaaggccc agtcaacttt
     7501 attctgcgga atatcgtgag actccggcag tgcgtaagcc tgtaattttg gaatagccat
     7561 cgtgttctct ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat tgttttttat
     7621 cgactttccc gaccgccgtc agcggaagtg aatccacaca ctccacgcga tccggtaatt
     7681 taaattcggc aataccctgt tcacgcagga aacgacgcac ctgcaccgcg cgcagcggct
     7741 cttttaccac cagataagca cagctttttt cgcccatcag ctcattttcc atgctcacca
     7801 gtgcggcata gatcactgcc ggatggctca gcagcaggtt ttcgatatct tcggcagcga
     7861 tcttctcgcc gccacggttg atctgatctt tctcgcgccc ctgcacggtg atgtaaccct
     7921 ctggatcaat agagatcaga tcgccggaac agtaaaaacc gttggcatca aaggcactgg
     7981 cattgtgctg cggacttttg taatagccac ggaaggtgta cggcccgcgc gtcatcaggc
     8041 gtccaacttc cccttgcggc agtggatttc cttcggcatc ggcaacccac acttcatcgt
     8101 ccggacacat tgggtaaccc tgggtatgga taattttctc cgcgctatca tcaagacggg
     8161 tgtagttcac cagcccttcc gccatgccaa acacctgctg caactggcag ccaatctcag
     8221 cgggaatacg cgctgcaagc gtggcagaaa gacgtgcgcc gccgacctgt aacagtttca
     8281 gcgaggcaag ctgcgcccgg ctttcgcctt cagccagcgc ctgcaaccac aggctgactg
     8341 ccggcggcac cagcgcggta acgttaacct gatgttttgc aatcaacggg aagcaaagcg
     8401 tggcgctggg atcggccgcc agaacaaccg ttcctccggc aagaaagacg cccagcgatc
     8461 ctggcgaact catagtgtag ttatgagccg ccgggatcga gcacaggtag cgcgtctgtt
     8521 gtgtgaactg acaaatctcg gcgctacgac gcacgctgta gtaatagtcg ttatgagtgc
     8581 gcgggatcag tttcggtgtg ccggtggtgc cgccggaaag ctggaaatag gccacttcat
     8641 cagcaggtga tggcgtggca gtaaaaccct ctgccggatg gttaatcgca tcctgcaagt
     8701 tatgctcacc gctgtcgttg tgcagttgca ccacgcgaat ggaagaatgc tctgcaacaa
     8761 atgtgttgag gaaatcatcc ccgctaaata gcgcatgctg gcgatcggca atcagtaacg
     8821 ccggttcaat ctggctggcg taggcgttca gttcactacg ctgatggctg aacagcgcca
     8881 gcaccggcgc gacgcccagt ttcagcagcg cgaaaaaggt aatgtaaagt tcagcgacgt
     8941 tacccagttg taccagcgcg gtttcaccag gtttaatgcc ctgacggcgc aaactacagg
     9001 cgaggttatc cgccgcctga ttcagctccc gataactcaa ctgccgctcg ccgtcgataa
     9061 ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc ggtcagtggc aaatcctgcc
     9121 agtagccttt ttcccgatag cgacgggcaa actcttccgg ccagcgggtg aatggaatgc
     9181 tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg
     9241 tttcgcgcca ctcacccaac ggtgacgacg caggcacaat ccccgctccg gcaaacagac
     9301 gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg ccgttacctt
     9361 cgctgtcaca ccaacccaca atgccgccaa acagttcgcg gtcgaacggc tccagttcag
     9421 caataacctg ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtc ggatgcagca
     9481 gacaggccag agtcagtgcg ttttcttgcg aattcgcttt accttcaaag ggagttgcga
     9541 gatgccacag cgtcggcgtg gtaatcaatt gtggagagga gggaacgtgt aactcactac
     9601 tgcgttcgcg cagtacctct ttcatcgcct gagtccccag ttcatgttca tggcgatctt
     9661 tttctgacgc cagcagacga ttacccgctt cgcgatcgag cacttcatcc ggctgacgac
     9721 gcgcggaacc ggctaacgga atggagctaa aacgctcgcc gtctttacgt agcagcagtt
     9781 ccgggctggc ccccagcagg acgccaccat cagccagcgg gacatggaag ttgtaactaa
     9841 ccgggttttg cgcaatcaac cgttccagca atacgccact atcaatggcg gcgtcagtgg
     9901 tgatatcaat caatcgtgac aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg
     9961 cggcgcgggc acccatctgt tcaaacgtgg tttgttcagg aattgccagg cgttccacca
    10021 cgttcagcga ctggctgcgg gtaaaacagc gggctgaggt ctgtttttcc tgacgggaga
    10081 acgactgcta ggattcggga atatacagcg acgaaggctg acgtggatcg aagggaatcg
    10141 ccccgaccat caccggattt ttgatgccct gcgctttggc atcggcaaac agcgcggcga
    10201 gtttttgctg gaagggactg tcgggcgaat ccccgttcac agccggttca tcgaaacggg
    10261 cgaaacatcc tgacgtcgta aaactgcggt acggcgacat aaagaaaaag cgattgggcg
    10321 caagtgttgc catggtctgc tgtacttcct cagccagtga cgtatccata tcatcctcca
    10381 caaaatgata aaggctttaa taatgattat cacttatatt ttcggccgct aacctaaaag
    10441 caaacgcgca ctatgtcaac tcttgaggta acgcaatctg caactcgggg ttgcatctgg
    10501 ttgtggttac aatgaaaatg agaagcatta ttgatgaatt cgcataagcg caatgtgatg
    10561 tcctgcgccg ttttgccccc tctccccaaa gggacggatt gtgcgctttg tcgaatttgt
    10621 cattacgccc ttaaccttat taataacagg aagctgactt gtgagactcg ccccgctcta
    10681 ccgcaacgcc cttctattaa caggactttt gctttcagga atagccgtag ttcaggccgc
    10741 cgactggccg cgtcagatta ctgacagccg gggcactcat accctggaaa gccagccgca
    10801 gcgtattgtt tccaccagcg tcaccctgac cggatcactg ctggcgattg atgctccggt
    10861 gatcgccagc ggcgcgacca cgccgaataa ccgcgtcgca gatgaccagg gctttttacg
    10921 ccagtggagc aaggtggcga aagaacgcaa actgcaacgg ctctatatcg gcgaaccgag
    10981 cgccgaagcc gttgccgcgc aaatgccgga tctgatttta attagcgcaa ccggcgggga
    11041 ttcggcgctg gcgctgtatg atcagctttc caccatcgcc ccgacattaa tcatcaatta
    11101 cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa
    11161 acaagcggca gagcggattg cacagtttga taagcaactg gcagcggcga aagagcaaat
    11221 caaattaccg ccgcagccgg tcactgccat tgtctatacc gccgctgcac acagtgccaa
    11281 tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc
    11341 gaaattgccc gcagggttaa acgccagcca aagccagggc aaacgccatg acatcattca
    11401 gcttggtggg gaaaatctgg ctgcggggtt aaacggtgaa tcgctattcc tgttcgctgg
    11461 tgatcagaaa gatgccgatg cgatttatgc caatccactg ctcgcgcacc tgcctgcggt
    11521 acaaaacaag caggtttatg cgctgggaac cgagacgttc cgcctggatt actacagcgc
    11581 catgcaagtg ctggaacggc ttaaggcgct gttttaagca ttaactgtcg gacgctgtca
    11641 cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc
    11701 cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgcgc
    11761 ccaaaccacc cagcagcgcc gcgcctatgg catcgcccgt cacgttctgc gccgtccaca
    11821 aaccgttaat ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt gtatattgca
    11881 gcaacgaact aaccgcgctc agccagccga acagcgccag acaaaccacg cctaaaatcc
    11941 acatcggcat caggccaaac agaccaatgg cgaggaacga tcccagcgtg gagagcagca
    12001 tcaataaccc tggtcgcgca ctatgtgcca gcttcccgct ggttaacgca ccaatagccg
    12061 cgccgagcgg gatcgccgcg tagagaaaac caatctgtgc cgctgacatc tgccagttgt
    12121 cagccagcgc cggatacagt accagcaccg cgctcgccat cgtcaataaa ccacccagca
    12181 gcgggctggc aagcagaaaa cgaaatcctg ccagtaatga tttcaacgga tgctcaagcg
    12241 gctgcggtgg cggtggcaac tccggaaggc ttaataacgg taacaaggta ataaacgtgc
    12301 ccgccgccgc cagcccgtag ttccaggcta cgccgccggt cgccagcaat aaaccgccaa
    12361 tcatgggcga aatcaccgac cccaggcgca cggtcaacat ggtgatcgcc ccggcctgca
    12421 ttaagttttc acgccctacc agtgcagatg ttgccgccag tagcgccgta acaccaagtg
    12481 atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac ggctccggca
    12541 gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc gccagcaaaa
    12601 tcactttttt gcgttcatag cgatccgcca gcacaccgcc aaccatcagg ccaacaaaca
    12661 tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgg gtcatcatct
    12721 ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag ataaaacgag
    12781 cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta agcagccagg
    12841 attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt cttaggctgc
    12901 acatgttaac atatccaaat aagatcgata acgataatta atttcattat catggaagtt
    12961 cgtatatctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt gctgttatta
    13021 ctgattatcg cgacggcatt aagcctgctc attggggcaa aatcactccc cgcttccgta
    13081 gtgctggagg ccttctccgg cacctgccag agcgccgact gcaccatcgt gctcgacgcg
    13141 cggctgccgc gtacccttgc cggtttactg gcaggcggcg cgcttggcct tgccggggcg
    13201 ttaatgcaaa ccctcacccg aaacccactt gccgaccccg gcttgcttgg cgtgaacgcc
    13261 ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcatc cgcacaggaa
    13321 caactggcga tggccttcgc cggggcgctg gtggcctcat tgattgttgc ctttaccggc
    13381 agtcagggtg gagggcagtt aagtccggtg cgtttaaccc tggcgggcgt ggcgctggcg
    13441 gcggtgctgg aaggactgac cagcggcatc gccctgctta atcctgacgt ctacgatcaa
    13501 ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt aaaagtggtg
    13561 ctgatcccgg ttctgatcgc cggagcaact gcgctattac tgagtcgcgc gctgaacagt
    13621 ttgagcctcg gcagtgacac cgcgacggcg ctgggcagtc gcgtggcgcg cacacagttg
    13681 attggtctgc tggcgattac cgtgctttgt ggtagtgcga cggcggtagt tggcccgatt
    13741 gcctttatcg gcctgatgat gccgcacatg gcgcgctggc tggtaggtgc cgatcatcgc
    13801 tggtcgctgc ccgtcacgct acttgctacc cctgccctgc tgctgtttgc cgatatcatc
    13861 gggcgggtga ttgtgcccgg cgaactgcgc gtttctgtgg tcagtgcgtt tattggtgca
    13921 ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gtgcatgatt tacgtctctc
    13981 gccgattact catcacctgt ttgctgctga tttccgcctg tgtggttgca ggtatctggg
    14041 gattacgcag cggtgccgtc acgctggaaa tctcgcaggt attcgccgcg ttgatgggcg
    14101 atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc gtgctgatgg
    14161 cgctgttgat tggcgcggcg ctgggcgtca gcggcgcgat ttttcagtcg ctgatgcgta
    14221 acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc ggcgtgctgg
    14281 tggcgatggt gctgtttggt caggatctga cggctatcgc gctggcagca atggtgggcg
    14341 gcattatcac ttcgctgttg gtctggctgc tcgcctggcg taacggtatc gacacctttc
    14401 ggttgattat tatcggtatc ggcgttcgcg ccatgctggt ggcctttaat acctggctgt
    14461 tgctgaaagc gtctttagaa acggcgctaa cagcaggttt gtggaatgcc ggatcgctca
    14521 acggcctgac gtgggcaaaa acctcgcctt ccgcacccat cattatattg atgctcattg
    14581 ccgccacctt gctggtgcgg cgaatgcgct tgctggagat gggcgatgat accgcctgcg
    14641 cgctgggcgt cagcgtcgag cgttcgcgtc tgttaatgat gctggttgca gtggtgctta
    14701 ccgctgcggc aacggcgctt gccgggccga tttcctttat tgctttagtc gcgccgcaca
    14761 ttgcccgacg cattagcggc accgctcgct gggggctgac ccaggcggcg ctgtgcggag
    14821 cgctgttact gctggcggcc gttctctgcg cccaacaact gtttatgccg tatcaacttc
    14881 cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg ttaattcagg
    14941 agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt taaccctggg
    15001 atatggcaaa tataccgttg cggaaaatct gactgtagaa attcctgatg gtcacttcac
    15061 ggcaattatc ggaccaaatg gctgtggtaa atccacgtta ctgcgtacct taagccgcct
    15121 gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac attacgccag
    15181 taaagaagtt gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc cgggcgatat
    15241 caccgtgcag gagctggtgg cgcgtggacg ttatccgcat caaccgctgt ttacccgctg
    15301 gcgcaaagag gatgaagaag cggtaacgaa agcgatgcag gccacgggaa taactcatct
    15361 ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgagcgt ggatcgcgat
    15421 ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct ggctggatat
    15481 cagtcatcag attgatctac tggaactgtt aagcgaactg aaccgcgaga aaggctatac
    15541 cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc atttgattgc
    15601 attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca ctgctgaact
    15661 gattgagcgc atttatggtc tgcgatgcat gatcattgac gatccggtag ccggaatgcc
    15721 gcttgtggtg ccgctcggac gaacggcacc ttcaaccgca aatagttaaa ctaagtggtc
    15781 tgccatcatg gcatcctgtt ttctggatgc catcgcatag cgcaataaca cgctaccaca
    15841 agccaccatc ccgccgatca gcgcggaaag gatcacaata atcgccttac ccggaccgtc
    15901 ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat tcacatcgtt
    15961 gacatttgct tttgttaatt gctcgacaag atactgccga ttacgtaatt caccgttcag
    16021 ttccgcaacg tcagtaaccg ctttttcaat ttccagtttg cgttcaatac cgtctgcgcc
    16081 gagggaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt atacgggctt
    16141 tttaattcct gccgcgttgg caatgtcgag tgaataattg aggcgctgaa tgtttgcatc
    16201 aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa actgggtttt
    16261 gatctccagt ttattacgga cgttttctat cgactctttc accaccaacg cagagatata
    16321 atcgatatac ccgctcaaaa cggtctgcgc ctctttactg gttggcgcgg taaaacttag
    16381 cgtccaggat gtatacagtg acggttcatc ttttttctta ctagcattgt catcaaccgc
    16441 tttcattttt tcgctcaatg caacaatagc gcgatgcaaa tccagttcat cgattttcgc
    16501 ctcttttaat tagtccatca cataaggtga tgaacgcagg tactcttcca gcaagctaac
    16561 agactgaaac ttcttgataa acaggttaaa tgcaccgcca cggtcgatat taacatcgag
    16621 atcaagaacg cggagtttgg taaaggtttt ctccaggtcc tgccactgaa tggcttcggc
    16681 gggagtaata acggctgagc tggtccattt ttgcggcagg atgaaagaga tcagtaaacc
    16741 tgcgcaggca aatgcaaaaa cgaccgccat gaccgttttt ttggcccgcc ataaaacttc
    16801 gattagatta agtaaatcaa tttcattatt actgggcgac gccagaggat actcagaaaa
    16861 atgagcgtca cttccctgtt taatattcaa tgatgacatg gggtataacc tgaaaatgag
    16921 tccaataggg gaaaatctcc acggcgctca ctttaccagt tggtgagaat tatccgaagc
    16981 tgaagtttgt aaattccttc cactgaatgc aacattcact ctatttaaga gaaatattca
    17041 tcaaacctat aaatcaaaca aataaaaaac ccataattat tttctgtaat
//
LOCUS       DAJTRD010000084        17052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTRD010000084 DAJTRD010000000
VERSION     DAJTRD010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.084"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..189)
                     /locus_tag="OO315_003028"
     CDS             complement(<1..189)
                     /locus_tag="OO315_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5891849.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
     gene            complement(294..530)
                     /locus_tag="OO315_003029"
     CDS             complement(294..530)
                     /locus_tag="OO315_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5891850.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(648..2087)
                     /gene="rsmF"
                     /locus_tag="OO315_003030"
                     /pseudo
     CDS             complement(648..2087)
                     /gene="rsmF"
                     /locus_tag="OO315_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            complement(2167..4787)
                     /gene="yebT"
                     /locus_tag="OO315_003031"
                     /pseudo
     CDS             complement(2167..4787)
                     /gene="yebT"
                     /locus_tag="OO315_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            complement(4756..6039)
                     /gene="yebS"
                     /locus_tag="OO315_003032"
     CDS             complement(4756..6039)
                     /gene="yebS"
                     /locus_tag="OO315_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5891851.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            6169..6666
                     /gene="msrC"
                     /locus_tag="OO315_003033"
     CDS             6169..6666
                     /gene="msrC"
                     /locus_tag="OO315_003033"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5891852.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6763..7461
                     /gene="proQ"
                     /locus_tag="OO315_003034"
     CDS             6763..7461
                     /gene="proQ"
                     /locus_tag="OO315_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5891853.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7481..9529
                     /gene="prc"
                     /locus_tag="OO315_003035"
     CDS             7481..9529
                     /gene="prc"
                     /locus_tag="OO315_003035"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5891854.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9721..10602
                     /gene="htpX"
                     /locus_tag="OO315_003036"
     CDS             9721..10602
                     /gene="htpX"
                     /locus_tag="OO315_003036"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5891855.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10648..12021)
                     /locus_tag="OO315_003037"
     CDS             complement(10648..12021)
                     /locus_tag="OO315_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5891856.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            12198..12989
                     /gene="kdgR"
                     /locus_tag="OO315_003038"
     CDS             12198..12989
                     /gene="kdgR"
                     /locus_tag="OO315_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5891857.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(13132..13371)
                     /locus_tag="OO315_003039"
     CDS             complement(13132..13371)
                     /locus_tag="OO315_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891858.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            13530..13673
                     /gene="mgrB"
                     /locus_tag="OO315_003040"
     CDS             13530..13673
                     /gene="mgrB"
                     /locus_tag="OO315_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5891859.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            13748..14035
                     /locus_tag="OO315_003041"
     CDS             13748..14035
                     /locus_tag="OO315_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5891860.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            14705..14848
                     /locus_tag="OO315_003042"
     CDS             14705..14848
                     /locus_tag="OO315_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5891861.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            14861..15070
                     /gene="cspE"
                     /locus_tag="OO315_003043"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             14861..15070
                     /gene="cspE"
                     /locus_tag="OO315_003043"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5891862.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15236..16045
                     /gene="rlmA"
                     /locus_tag="OO315_003044"
     CDS             15236..16045
                     /gene="rlmA"
                     /locus_tag="OO315_003044"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5891863.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16042..16608)
                     /gene="mntP"
                     /locus_tag="OO315_003045"
     CDS             complement(16042..16608)
                     /gene="mntP"
                     /locus_tag="OO315_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5891864.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGAPLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT         4512 a         4119 c         4295 g         4126 t
ORIGIN      
        1 aaaatcacca tactcctgtg ctggttgcca gaaaccatct ataaaatcct caatcggaaa
       61 acaactgcca tggcggatcc gttgatcgct catagaataa agacactgct gttccgtgtt
      121 gtagacatcc acaacaatat cttcacaacc gccatccagg tagcaaacaa aaagtaccag
      181 cgcgaacatt tcatccccga agtgtggtgc cgtaccgtta agtttaggag agattttaca
      241 acgggggaat aaccaggaca aataacccgc catgccgacg ggttcttttt ggatcaggca
      301 agacgcataa tccaggcatc agattgttga tcgtaacggg tacggtaaag gacagagtgt
      361 tcgccattta gcagggcagg gatactggtt tcagcatccc aggcatccag ttgcatacat
      421 aaatcggggt cagatttaca aggaatggtt aacgttcgat cacccggcga ttcaccgtgt
      481 aattcgccgt cgtcgatttc aaaagcgcct atgcgcacac tggttttcat cttcgtgctc
      541 tccatttagc tgctgattgt agtacgacca gtccgatcgc acaatttttc agcctagttg
      601 aggccggcga atgtgccagt caaaaagtgc gctttttttg tgcgctgtca tgcgttactg
      661 gtaaaaagtt tcccgtcccg caccagttca cgcgggtagc tgtttttcag tcgcgaacca
      721 atccgtttgg ctaaaccaat cggctgatgc tggaaagtaa ccaacacgtc atccgccact
      781 ggcgcggctt gcgggtaaac atcgcgcccg cgataccact cctacgcttc ctgcggtgtc
      841 agttcaaaag cgtttacatt gtcgggggag gcatgggcaa taaccgcttc atgctgccag
      901 cgataacctt tgttatgcgt ttcggcaagt ttaatcccta agcgagaaaa tcggacttta
      961 ccgatcaggg cttcaatgcc taccgggaac aaccacagtt ctttgtcgcg ttgccagagt
     1021 cgcaggtttc catcccagtt taagccaaca cttgcagccg cctgacgaat ttgtccggct
     1081 tcgcgatctt tcaccgggct gaacgggaaa ttgcccactt tgtattttgg ggcgggtaag
     1141 gcggggatcg cctgagtttt acgcagacga gcaacgaaga agccttcgca gtcgtaaatt
     1201 tgtgggaaaa catgcaaaaa accttcttcg gtcagcgcct tgtttgcacc agggaagaga
     1261 tcgccaagtg gtaaaaactc tacttcgtcg gggtaagtct ctttcagcca ccggcaaacg
     1321 gcttcgtttt cttcccggtt taaggtatag gtcgagtaaa ccagcgtacc gccaggacgt
     1381 aatgcatgaa aggcgctgtc aatcagttcc cgttgggtag ctgcgatttc ctgattgctt
     1441 tcgggtgacc agttttttag cgcatcggga tctttacgca ccacgccttc gccggagcag
     1501 ggagcgtcca gcaaaatggc atcaaacatt tctggcactg ccgcaccaaa cacgcggcca
     1561 ccaaaatgtg tgagcgcaac attactgatg ccacagcggc tgatattggc atgtaacact
     1621 tttacccaac tggcggaaaa ctcattggca aggattgccc cttcgttatt catccgcgcg
     1681 gcaatttgcg tcgtttttga gcctggcgca gcagcgacat ccatcacccg ctgtggtgca
     1741 ttaccgtcag caaacaaggc ggcgacgggc aacattgaac tggcttcctg aatataaaac
     1801 aggccactta aatgctcggt ggtactaccc aatggcaatg catcttcatt gtcgcgttca
     1861 atccagaaac cttcttcaca ccacggaatt ggcgtaagcg tccagccata aggagcggtt
     1921 aattgcagga aatcagcaac ggagattttc agcgtattaa cgcgaatgct gcggcgcaac
     1981 gggcgctgac aggcggcaag aaaatcatca aatgagagcg tcgaaggcat agcttcgcgc
     2041 atttgtgtca gaaacgcgtc cgggaaataa acggtgtgtt gggccacgag catgtaccac
     2101 tggcaaaaaa acaggcgcgc agtttagcat aagcgctccg gcgcaggcac gccggagcag
     2161 tgggcattat ttgggaagcg cagttcccca ttcacgccac tctttcggtt cactttcctg
     2221 caacaggaag tgtttgcctt cttgggcttt cggtgcaagt ggcgtacccg gaggcgtggc
     2281 gaaggcgatg ccgccacgga taaattggtt aaaggtgccg gttttcacta cgccgcccgt
     2341 cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt tacgcaccag
     2401 gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca atgtccccag
     2461 cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg taccgatgcc
     2521 taacgaaccg gcttccggcg cttcaacaat aatgcttaaa ccatctaggt aacgcgaatc
     2581 agtaatggtg gcctcttcta attcaaagtc gcgacgagga ttgccacggc ctggttcgac
     2641 gttgatatac ggctggagga tagtatctag atgctcaacg ccagctgccg agatttgcgg
     2701 tgtgaccact gagaagcgcg taccaccgcg agcaaaggtc tggacatatt ccggatagag
     2761 caccgccttt gcctgtactt cattgcgtgc ggtaatcaga tccagcgtct ggatttgccc
     2821 gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg catcgaaagc
     2881 gtgaagcgta atctgcctgc caaccgcacg ggccgctgtt tcggaagcat acagaattcg
     2941 cttgtcgcct ttacgctgac tggcgctggc accactgagg ttatcgaagc taatggcccc
     3001 ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat tcagctgaac
     3061 tttcgccccg ccttctgccc agaacacgct gttgctggtc aaaaggttgc gatattccgg
     3121 tttaatgtgc agatcgatat cgaacgcgtt agctcgcgga cgcacggtaa taacttcacc
     3181 aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca gcgtctctgc
     3241 actcaaactc atggttgtgg tgggtaaatc gctaaggctg ttctccagcg ctttttccag
     3301 attggcatac agtggatagc tggctttcat ctcgccttta tcgcccggca gaatacgtat
     3361 cccgccgtta atccattctg aggcgctggc accgagaaac tcaacgccat ccagccccac
     3421 cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca gttctcgatg
     3481 ctgaggctcg atggcgacgg taaaggtgac gcctttgctg gtgagtttac gatcgataac
     3541 ctggcctacc tgcacgccgt gaagaatgag cggctgaccc gcatcaatac cgtaactttc
     3601 cggtgctgtc agggtcagtg tcagaacgtc cggctcttgc agcagggctt tttcgcccgg
     3661 cacaacaacg aactctttgc gtggctcgcc atcgccgggc accaactcaa aggtttttcc
     3721 ggtcagcagg gcgctgagat tagcatcgct aagggataat ttcgggttgc gtaattcgat
     3781 gcgggtattt tcacgaagca gggatcaacg gtcatttccc cagtgacttt accaccagga
     3841 tttaaatcca gtttagtcag ctgtccgact tccagcccct gatacattaa cggcgtcgag
     3901 tcggcggtta atcctgctcc actcggcagt tccagtttta ttattactcc acgctggctg
     3961 tgggccagat cttcatacag accaaaggta tcttccgcct cggcaggttt cgactcttca
     4021 ggtgaatcga aggcaatcgc accgttaacc agtgccgcca gactttccag tttcaccttc
     4081 gcgccactga tactgacgtt ggcatcaacg ccggaaacgt ttcagaaacg gctacctttt
     4141 ttcaccagat cggtaaaacg ccgctcgatc aggacatcaa tcaccacgcc ttgcttgttg
     4201 ggattgatgg catagtcgta gacttttccc accgggatct tgcggaaata gaccaatgaa
     4261 ccgctgttca gcgaaccgag agcgggggct tgcaggtgga tcatcagatc gccattgtcc
     4321 agccgatatt tcggttgggt atcgagcgcg acaaagtgat cctgctcttt acctttaccc
     4381 ggcatcatgc cgatatagtt cccaccgacg agggcgtcca gcccggagac acctgccaac
     4441 gatgcttttg gcgtcatcag ccagaactga gtctcttcgc gcagcgcatc tttcatatcg
     4501 gacttgatgc tgaccttgac ttcaatctta cgaagatcgt cgctgaggct gatatcctgc
     4561 actgttccga cttcaacgcc ctgataacga acaggcgtac ggcccggaac aataccatcc
     4621 gccgacataa agtcgatggt gacggtatta ccccggtcct gataactgtc ccaaatcagc
     4681 caaccggcaa tcattagcgc gatgaaaggc agcagccaga aaggtgagat acggcgttta
     4741 tttttaatct gcgcttcagt cgtcgaagcg ggcgtttcct gactcatgtg catcccaaag
     4801 taagcggctg tccagccatt ccacagcaag aatagtcaaa attacggcag cgccaaaata
     4861 aaacgcagcc ggtcccatag taaaagcgag gatctgatcg cgattaatca gcgacatggt
     4921 taaagatatg acaaacaggt cgagtatcga ccagcgtcca atccaggtca ccatccgcag
     4981 taacagaatg cgtgtgcgta aaccttgctg gcatttaaaa tgaatgctca acagtaaagt
     5041 gaacatgacg atcactttag taaacggtac cagaatactg gcgataaaca cgattcccgc
     5101 aaccgcaatg ttgctacttg ccagcgacat aattccggaa agaatcgtat cttcctgccg
     5161 gccaccattc agataaatga tagaaatagg caataggttg gctggtaaca acaaaacgat
     5221 tgacgctaac agcgccgccc agcatttttg caaactatga cgacgacgca ggcgtagcgg
     5281 gatatggcaa cgcgggcaga gaccacgctg atcaggatag ccggtaaaat ggcacccaag
     5341 acagacacga agtttctcgt ccctacgcgt agcggggcgc tgcggataat atcgctccca
     5401 cagctcctca acattaagat gtgacaacgt caccgtcgtt aaaatcacca acgccacaaa
     5461 agagaacaag ccgacacccg cctggatatg ggcataatcc tgtaccttta tagaagcaac
     5521 gccaatgccg accaggtaga tatccagcat tacccactct ttaagtcgct caagcatcag
     5581 cagtaccgga cgtaaattca ttcccagtcg gttaccaaac cataaatatg ctatggagga
     5641 caccagaatg aggggggcac caataacgca gaaaaagacc attgatcccg ttatcgcatc
     5701 gccctgtttg gtcatttgcc agatgccttg catgacgttg gcgtcaatac gaatgcctaa
     5761 cagccagata tgcaacagcg gttcgcccca ggcaaacggc ataagcaaca gcatggtgaa
     5821 ggccattgcc gccaggcgcg ttagcgacca gtcgcgcccg tcgcgaattt ttgcctgaca
     5881 gcgcggacaa taggcacttt gatgagaatt tatctcgggc aggctaaaca gcatgtcaca
     5941 ttgcgggcaa cgttggtaat caccacgcgg cagttcctcg ccgattgccc tcactgttat
     6001 cttttttgtc ggcgtaattt gtggtgtgtt aagagccatg acgtgccaaa tgtaataaga
     6061 attaacatta atcttaaccc atgatgggaa tgatcttgag cattaacgca tttaatgctt
     6121 attttaatag gctggcagtt ccaggtaaga caaccaagtg attaagtaat gaacaaaaca
     6181 gaattttacg cggatttaaa tcgcgacttt aacgcgctga tggcgggaga aaccagtttt
     6241 ctggcaacgc ttgcgaacac cagtgcgttg ttatacgagc gtctcactga cgttaactgg
     6301 gcaggttttt atttgcttga ggacgataca ctggtactcg gaccatttca gggcaaaatt
     6361 gcctgtgtcc ggatacccgt cgggcgcggg gtgtgcggca cagcggttgc ccgcaatcaa
     6421 gtgcagcgta tcgaggatgt tcacgcgttt gacgggcata ttgcctgtga tgcggcgagt
     6481 aattctgaaa ttgttctgcc gctggtggtg aaaaatcaga ttattggtgt tctcgacatc
     6541 gatagtactg tcttcgctcg ctttacagac gaggacgagc aaggcttacg tcagcttgtg
     6601 gcacagcttg aaaaagtgct tgcaacgacg gattacaaaa aattctttgc gagcgtcgca
     6661 ggataatcaa cggataacgt agcatttact ggtagcgtca ttataatgac gcctgttcat
     6721 gcctgcgctt gttggctacg tccgttgtaa tcaggaaatt tcatggaaaa tcaacctaag
     6781 ttgaatagca gtaaagaagt aatcgcgttt ctggccgaac gttttcccca ctgtttcagt
     6841 gcggaaggtg aagcgcgtcc gctgaaaatt ggtatttttc aggatttggt cgatcgtgtt
     6901 gccggggaaa tgaacctgag caaaacgcaa ttacgatccg ctttacgtct ctacacttcg
     6961 agctggcgtt atctttacgg tgttaaaccc ggcgcaacgc gtgtcgatct tgacggcaac
     7021 ccatgcggtg agctggacga gcaacatgta gagcatgctc gcaagcagct tgaagaagcg
     7081 aaagcgcgtg ttcaggcaca gcgtgctgaa cagcaagcga aaaaacgcga agctgccgca
     7141 gctgctggtg agaaagaaga cgcaccgcgc cgcgaacgca agccacgtcc gaccacgcca
     7201 cgccgcaaag aaggcgctga acgtaaacct cgtgcgcaaa agccggtaga gaaagcgcca
     7261 aaaacagtaa aagcacctcg cgaagaacag cacaccccgg tttctgacat ttcagctctg
     7321 actgtcggac aagccctgaa ggtgaaagcg ggtcaaaacg cgatggatgc caccgtatta
     7381 gaaatcacca aagacggcgt ccgcgtccag ctgaattcgg gtatgtcttt gattgtgcgc
     7441 gcagaacacc tggtgttctg aaacggaggc cgggccaggc atgaacatgt tttttaggct
     7501 taccgcgtta gctggcctgc ttgcaatagc aggccagacc ttcgctgtag aagatatcac
     7561 gcgtgctgat caaattccgg tattaaagga agagacgcag catgcgacgg taagtgagcg
     7621 cgtaacgtcg cgcttcaccc gttctcatta tcgccagttc gacctcgatc aggcattttc
     7681 ggccaaaatc tttgaccgct acctgaatct gctcgattac agccacaatg tgctgctggc
     7741 aagcgatgtt gaacagttcg cgaaaaagaa aaccgagtta ggcgatgaac tgcgttcagg
     7801 caaactcgac gttttctacg atctctacaa tctggcgcaa aagcgccgtt ttgaacgtta
     7861 ccagtacgct ttgtcggtac tggaaaagcc gatggatttc accggcaacg acacttataa
     7921 ccttgaccgc agcaaagcgc cctggccgaa aaacgaggct gagttgaacg cgcagtggga
     7981 cagtaaagtc aaattcgacg agttaagcct gaagctgaca ggaaaaacgg ataaagaaat
     8041 tcgtgaaacc ctgacacgcc gctacaaatt tgccattcgt cgtctggcgc aaaccaacag
     8101 cgaagatgtt ttctcgctgg caatgacggc gtttgcgcgt gaaatcgatc cgcataccaa
     8161 ctatctttcc ccgcgtaata ccgaacagtt caacactgaa atgagtttgt cactggaagg
     8221 tattggcgca gtgctgcaaa tggatgatga ctacaccgtt atcaattcga tggtggcagg
     8281 tggtccggca gcgaagagta aagctatcag cgttggtgac aaaattgtcg gtgttggtca
     8341 aacaggcaag ccgatggttg acgtgattgg ctggcgtctt gatgatgtgg ttgccttaat
     8401 taaagggccg aagggcagta aagttcgtct ggaaatttta cctgctggta aagggaccaa
     8461 gacccgtact gtaacgttga cccgtgaacg tattcgtctc gaagaccgcg cggttaaaat
     8521 gtcggtgaag accgtcggta aagagaaagt cggcgtgctg gatattccgg gcttctatgt
     8581 gggtttgaca gacgatgtca aagttcaact gcagaaactg gaaaaacaga atgtcagcag
     8641 tgtgatcatc gacctgcgta gcaatggcgg tggggcgttg accgaagcgg tatcgctctc
     8701 aggtctgttt attcctgcgg gtcccattgt tcaggtccgc gataacaacg gcaaggttcg
     8761 tgaagacagc gataccgacg ggcaggtgtt ctataaaggc ccgctggtgg tactggttga
     8821 ccgtttcagt gcttcggctt cagaaatctt tgccgcggca atgcaggatt acggtcgtgc
     8881 gctggttgtg ggtgaaccga cgtttggtaa aggcaccgtt cagcaatacc gttcattgaa
     8941 ccgtatttac gatcagatgt tacgtcctga atggccagcg ctgggttctg tgcagtacac
     9001 gatccagaaa ttctatcgcg ttaacggcgg cagtacgcaa cgtaaaggcg taacaccaga
     9061 catcatcatg ccgacgggta atgaagaaac ggaaacgggt gagaaattcg aagataacgc
     9121 gctgccgtgg gatagcatcg atgccgcgac ttatgtgaaa tcaggagatt taactgcctt
     9181 tgaaccggag ctgctgaagg aacataatgc gcgtatcgcg aaagatcctg agttccagaa
     9241 catcatgaag gatatcgcgc gcttcaacgc tatgaaggac aagcgcaata tcgtttctct
     9301 gaactacgct gtgcgtgaga aagagaataa tgaagatgat gcgacgcgtc tggcgcgttt
     9361 gaacgaacgc tttaaacgag aaggtaaacc ggagttgaag aagctggatg atctatcgaa
     9421 agattaccag gagccggatc cttatctgga tgagacggtg aatatcgcac tcgatctggc
     9481 gaagcttgaa aaagccagac ccgcggaaca acccgctccc gtcaagtaat atcaatcagg
     9541 cacaagaaat tgtgcctgat tttttaacag cggcaagatg ccgtaaatca gatgctacaa
     9601 aatgtaaagt tgtgtctttc tggtgactta cgcactatcc agacttgaaa atagtcgcgt
     9661 aacccatacg atgtgggtat cgcatattgc gttttgttaa actgaggtaa aaagaaaatt
     9721 atgatgcgaa tcgcgctctt cctgctaacg aacctggccg taatggtcgt tttcgggctg
     9781 gtactgagcc tgacagggat acagtcgagc agcgttcagg ggctgatgat catggccttg
     9841 ctgttcggtt ttggtggttc cttcgtttcg cttctgatgt ccaaatggat ggcattacga
     9901 tctgttggcg gggaagtgat cgagcaaccg cgtaacgaaa gggaacgttg gctggtcaat
     9961 actgtagcaa cccaggctcg tcaggcgggg atcgctatgc cgcaagtggc tatctaccat
    10021 gcgccggaca tcaacgcttt tgcaaccggt gcgcgccgtg atgcctctct ggttgctgtc
    10081 agcaccggtt tgctgcagaa catgagcccg gatgaagccg aggcggtaat tgctcacgaa
    10141 atcagccaca tcgccaatgg tgatatggtc accatgacgc tgattcaggg cgtggtgaac
    10201 accttcgtta tctttatttc ccgtattctg gcgcagcttg ccgcgggttt tatgggcgga
    10261 aatcgtgatg aaggtgaaga gagcaacggc aacccgctga tctactttgc ggttgcaacg
    10321 gttctggaac tggtgtttgg tattctggcg agcattatca ccatgtggtt ctcgcgtcat
    10381 cgtgaattcc atgcagatgc cggttcggca aaactggttg gtcgcgagaa aatgattgcc
    10441 gcgctgcagc gcctgaaaac cagctatgaa ccgcaagaag caaccagcat gatggctttc
    10501 tgcattaacg gtaagtcgaa atcgctcagt gagttgttca tgactcaccc gccgctggat
    10561 aaacgtattg aagctctgcg tacgggtgaa tacctgaagt aatatgaagt aaatactaaa
    10621 aagcgcgtcc tgatcgacgc gcttttttta tgccctggct cgtggctgag tgatacgtaa
    10681 accactgaca caggcagcaa tcactgccag aatagccgca gccatcagcg agacgtgcgt
    10741 accattatca ccaaactgat ttagcatcag cgccaccagc gccgcgccac tactctgacc
    10801 cagtagacga gccgttccta acatgccact ggccccaccg ctacgttcgc gcggagcgga
    10861 ggtaataatg gtgtggttat tgggtgactg gaataagcca aatccagcgc cacataagat
    10921 catcggccag ataatattga tatccgcagg tgacgcgggc agcagaacca gggaaaaaag
    10981 ccccgtagcc atgatgaaca accctaaagc acccagcaat cctgcatgta cgcgttcaat
    11041 caaatagcct gccagaggag ccatcaccat cgttgctaac ggccacggtg tcagaagtaa
    11101 acctgtttcg acttcactac gcccgagcac ggtttgcagg taaaagggca gggaaaccat
    11161 tgccagcatt tgtgcgcaga aagagcaaac agatgtgcaa atagaaagtg aaaacagcgg
    11221 gatacgcagt aaatccaccg gtagcagcgg tacgggaaga gaaagctggc ggcgaataaa
    11281 gaaaatacca acaacaacca ttaccaccag ttccgcagca attaacgtca gcgattgccc
    11341 ctgagcgaaa ccactcaacg cagtgataag caggccgaag gttaacgcgt tcatcacggc
    11401 gctgggcagg tcgaaacggg gtttactggc gcgagaacca ttgggtggca gaaaacgcat
    11461 cgccagaagc agggcgataa tacctaacgg tacgttgatt aaaaataacc atttccagga
    11521 tgcgatggag aggattgctg cagcaattgt cggcccggca gcagaagaga cggcaacaat
    11581 aaacgagttt atgcccatcc ctctccccag aaaacgttgt ggatagatca ggcggataag
    11641 tgcggtatta acgctcatca acgctgcacc gccgaaacct tgtatgacac gcgcaagggt
    11701 gagcatttgc agcgaatcag aaagggcgca gaacagtgaa gacaacagaa aaacgaccag
    11761 accgcattta taaatacgtc gatagccaaa catatcgccc agaaacgaaa acgagagcag
    11821 ggagatgaca atggcgattt gataggcgtt cactacccag atggaactgg ctggtgtggc
    11881 atgaaggtcc gtggcaattg ttggcagggc gacgttggcg attgcgccgt caaggacagc
    11941 catcgaaata ccaatcacaa tggttaatat cgcaccgtat cgctggggca atggcaggcc
    12001 gtcggcctga acttttggca taggaatttt atatctttgg tgaataatca acaggggtat
    12061 gattctagca ctgatattca cggtggatgt cgcagaattg taactaaccg gcaaaagatg
    12121 gattgcgacc accctggtgc gaaattataa taaaaaacag ttctgatttt tataaaacac
    12181 tcgcaatgag gtgataaatg gctaacgcag atctggataa acagcctgat tctgtatctt
    12241 ccgtgctaaa agtttttggc attttgcagg cgctgggtga agagcgcgaa atagggataa
    12301 ccgagctgtc gcagcgcgtc atgatgtcaa aaagcaccgt ttatcgcttt ttacagacca
    12361 tgaaaacctt aggttatgtg gcgcaggaag gggagtcgga gaaatattcc ctgaccctga
    12421 aattgtttga actgggcgct cgcgcgttac aaaacgtcga tttaattcgt agcgcagata
    12481 tccagatgcg tgagatctcc cgcctgacca aagaaactat ccacctcggc gcactggacg
    12541 aagacagtat tgtttacatt cacaaaattg actctatgta caatttgcgc atgtattcac
    12601 ggattgggcg tcgtaatccg ctgtacagca ccgcgattgg taaggtactg ctggcatggc
    12661 gcgatcgcga tgaagtgaag caaattcttg agggcgtgga gtataaacgc agtaccgagc
    12721 ggaccatcac cagtacagaa gcgttattac ccgttctgga ccaggtgcgc gagcaggggt
    12781 atggagaaga taacgaagag caggaagaag ggctgcaatg cattgcggta ccggtatttg
    12841 atcgctttgg cgtggtcatt gccggtttga gcatctcctt cccgacgttg cgtttctctg
    12901 aagagcgttt acaggaatat gtcgcaatgt tgcataccgc agcgcgcaaa atttctgccc
    12961 aaatgggtta tcacgactat ccgttctgat gagagtaaga acctgtcgga atatcaaaca
    13021 gacaggttct ttatttagca tgagaaaaat aaagttgaag gtgcgttata ttaaacgcgc
    13081 ttgctataag aatattttac tcatgagtga gaatctggtt atttattacc cttaaccatt
    13141 atcgaccacg atattgcttt tacgtaacag tgggcaatct gttatctcca agaaaccact
    13201 tttagtgtgc aagtattgta ccgtgctggt gcctctggca gtcagatagg tacattgcaa
    13261 acctaatcct gcggcattct ctttgcttcc aatcaaaacg ccatatccgc tgagtaataa
    13321 tcctatccat accagtgcta tcagcataac tgtgcgaatg atgaatcgca ttacaatctc
    13381 ttctcttttt atgttcgctt aatcgtagcg gcaatatgcg ctgaagcaag ctactcattc
    13441 cgaaaaagca cgaatatcga catagttagg cgctgtttaa ctaacgcatg ctagtttaat
    13501 gacataaggt aggtgaagcg gagattggag tgaaaaagtt tcgatgggtc gttctggttg
    13561 tcgtggtgtt ggcttgcttg ctgctttggg cgcaggtatt caacatgatg tgcgatcagg
    13621 atgtacaatt tttcagcgga atttgtgcca ttaaccagtt tatcccgtgg tgatatcatt
    13681 ttttcagcac tagattttct tcctctccag cagtggtaga atagcgcctc tactcagagg
    13741 tggtgaaatg aacgaagttg taaattcagg cgtgatgaac attgcgtctt tggttgtatc
    13801 ggtggtggtt cttcttatcg ggctcatctt gtggtttttt atcaatcgtg ccagttcacg
    13861 gactaacgaa cagattgaac tgcttgaggc gttgctggat cagcaaaaac gtcaaaatgc
    13921 actgttacgt cgtttgtgcg aagcaaacga accagagaaa gcagataaaa agaccattga
    13981 gagtcaaaaa tcggttgatg acgaagatat tattcgcctg gtcgccgaac gataatcctt
    14041 cctcaatctt gagtaacccc gtcgttgaag ggttactcaa ttcatcctga ttatacgcac
    14101 tgtcatggtg caactgcttc aaattaaaac tctggtctga cttcaaataa ttatcatatt
    14161 atttatttgc gtaacgggat atcagaaaaa gatattttcc tcattaattt attaggatgt
    14221 ttacatcaga tttgtgatta agcgtggtat tatttattag cgaaacgttt ctctcttgat
    14281 ttttttgctc actcatcaat ttttcttatt ttaaatttac aatccctttg gggattgact
    14341 tctctttagg gtaattgata gccgttaact gactgtttta tgagaaaaag tgatataact
    14401 ttttattcat tgcatagcag aaaatgtgat attgcacgca ctatgtaata acttctccct
    14461 ctggcctgga acaactgaac ttattgaact atgttagaaa ctacgccagt ttaagtatct
    14521 gcctgaactg gcaaggttaa gcacaatgat atatcggcgc gtattccgtt gcataagtgt
    14581 gcaaaaaaag tggaagacgt atcgagattt gtgcgtctga tcgagatatg tttaaaaatg
    14641 gcttgccata attaacgttg tatgtgataa aagatttcgg gttaaacgag gtacagttct
    14701 gtttatgtgt ggcattttca gtaaagaagt cctgagtaaa cacgttgacg ttgaataccg
    14761 cttctctgcc gagccttata ttggtgcctc atgcagtaat gtgtcagttt tatctatgtt
    14821 atgcctgcgg gcgaagaaaa caatctaagg aatttttcaa atggcaaaga ttaaaggtca
    14881 ggttaagtgg ttcaacgagt ctaaaggttt tggcttcatt actccggctg atggcagcaa
    14941 agatgtgttc gtacacttct ccgctatcca gggtaatggc ttcaaaactc tggctgaagg
    15001 tcagaacgtt gagttcgaaa ttcaggacgg ccagaaaggt ccggcagctg ttaacgtaac
    15061 agctatctga tcgaatccac tgatctgaag tgtgaatacg cttcaatctc gctataaagc
    15121 ctcgtcgaat gcgaggcttt ttactatgct ttatcttcgc tcctggcgtt cggatatttg
    15181 cccgccgcgt gattcgcgtt acacttgcgg cctttagtat cctgccggag ttgtcatgtc
    15241 gttttcctgt ccactttgcc atcagcctct ttcgcgtgaa aaaaacagct atatctgtcc
    15301 ccaacgacat cagtttgata tggcgaaaga agggtatgtc aatctgctgc ccgttcagca
    15361 taaacggtct cgtgatccgg gcgacagcgc ggaaatgatg caagcacgcc gcgcattctt
    15421 agatgccgga cattatcagc cgctgcgtga tgcaattgtc gcccaactgc gggaacggct
    15481 tgatgaaaag gccacggcgg tgctggatat tggctgtggt gaagggtatt acacacacgc
    15541 atttgccgat gcgttgcccg aaatcaccac gtttggcctg gatgtctcga aggtagcgat
    15601 aaaagcggcg gcgaaacgct atccgcaggt cactctttgt gtcgcttcca gccaccgttt
    15661 gccgttttcc gataccagta tggacgccat aatacgtatt tacgcgccgt gtaaagcaga
    15721 agaattagca cgagtagtga agcccggcgg ctgggtcatt actgccacgc cgggaccgcg
    15781 acatttgatg gagctgaagg ggctgattta caatgaagta catcttcatg cacctcatgc
    15841 agaacaactg gaaggtttta cattacagca gagtgcggag ttgtgttatc cgatgcgtct
    15901 tcgcggtgat gaagccgtcg cattattgca gatgacgccg tttgcctggc gtgcgaagcc
    15961 agaagtctgg caaacactgg cagcaaaaga agtgttcgac tgccagacgg actttaatat
    16021 tcacctctgg cagcgttctt attaaccgtg gaagtgcgtc cagaggatct ggacgccgat
    16081 gccgatcagc accagcccgc cgagaatttc cgcttttttc ccaataattg agccgataaa
    16141 gcgaccaacc atcatcccta atgtcgacat aatcaaggtt gcacaaccaa tggccaatgc
    16201 ggtcgcgata atgttgacct gaaggaaagc aagaccaaca cccacagcca tggcatccag
    16261 gctggtggca atcgcggtgg ttaccagtag ccagaaaccg tgccgacggc gcggctcttc
    16321 atcttcatca tctgcgccac gaaaaccctc aataatcatt cgcccgccga ggaatatcag
    16381 cagcacaaac gcaatccagt gattccattc aaggacaaac cggctggcta acatgcccat
    16441 tccccagccg atcagcggcg tcagggtttc gacggcacca aaaataaggc cggttcgcaa
    16501 tgcttcagaa aatttcggtt tatggagggg ggcaccttta ccgattgatg cagcaaatgc
    16561 atccatcgac ataccaaacg caagaagaac agtagcagtg atattcatga caacgtcctg
    16621 accggggtat ccataaaaca catcatcgcc cccagcaaac atgcatacat gaacaacaag
    16681 ccgcgatggg ttttcgcgtt catgcaggca tagcgattga tgtgcatatg gtctcgcctg
    16741 actgaaagtc ttcagtccgt acgtgccacg ttttgcaacg agtatgttga cacgtacatt
    16801 tccggaatct ggaaatcggc tactccccaa atgagggcgc aaccttaaca tattttgaga
    16861 atataaaaca actgttggaa tatattattt cattgcttta ttgataacga ttttcattta
    16921 tgtttatata tcaatgaaac gttaaaataa ataacaaata agggaagaga taatatagcc
    16981 taagctatgt ctgattatta aaaatacact aaacatagct ttggctaaat tcataagtta
    17041 ttgagttgat ac
//
LOCUS       DAJTRD010000085        16082 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTRD010000085 DAJTRD010000000
VERSION     DAJTRD010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16082
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.085"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO315_003046"
     CDS             complement(87..2063)
                     /gene="speA"
                     /locus_tag="OO315_003046"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5891865.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO315_003047"
     CDS             complement(2072..2203)
                     /gene="yqgB"
                     /locus_tag="OO315_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5891866.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2339..2554
                     /locus_tag="OO315_003048"
     CDS             2339..2554
                     /locus_tag="OO315_003048"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891867.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2858..4012
                     /gene="metK"
                     /locus_tag="OO315_003049"
     CDS             2858..4012
                     /gene="metK"
                     /locus_tag="OO315_003049"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5891868.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4449..5843
                     /gene="galP"
                     /locus_tag="OO315_003050"
     CDS             4449..5843
                     /gene="galP"
                     /locus_tag="OO315_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5891869.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5920..6417
                     /locus_tag="OO315_003051"
     CDS             5920..6417
                     /locus_tag="OO315_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5891870.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6512..7219
                     /gene="endA"
                     /locus_tag="OO315_003052"
     CDS             6512..7219
                     /gene="endA"
                     /locus_tag="OO315_003052"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5891871.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7299..8030
                     /gene="rsmE"
                     /locus_tag="OO315_003053"
     CDS             7299..8030
                     /gene="rsmE"
                     /locus_tag="OO315_003053"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5891872.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8043..8993
                     /gene="gshB"
                     /locus_tag="OO315_003054"
     CDS             8043..8993
                     /gene="gshB"
                     /locus_tag="OO315_003054"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5891873.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9102..9665
                     /locus_tag="OO315_003055"
     CDS             9102..9665
                     /locus_tag="OO315_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5891874.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9665..10081
                     /gene="ruvX"
                     /locus_tag="OO315_003056"
     CDS             9665..10081
                     /gene="ruvX"
                     /locus_tag="OO315_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5891875.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10257..11237)
                     /locus_tag="OO315_003057"
     CDS             complement(10257..11237)
                     /locus_tag="OO315_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5891876.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11255..11959
                     /gene="yggS"
                     /locus_tag="OO315_003058"
     CDS             11255..11959
                     /gene="yggS"
                     /locus_tag="OO315_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5891877.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11977..12543
                     /gene="yggT"
                     /locus_tag="OO315_003059"
     CDS             11977..12543
                     /gene="yggT"
                     /locus_tag="OO315_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5891878.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12540..12830
                     /locus_tag="OO315_003060"
     CDS             12540..12830
                     /locus_tag="OO315_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5891879.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12838..13431
                     /locus_tag="OO315_003061"
     CDS             12838..13431
                     /locus_tag="OO315_003061"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5891880.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13424..14560
                     /gene="hemW"
                     /locus_tag="OO315_003062"
     CDS             13424..14560
                     /gene="hemW"
                     /locus_tag="OO315_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5891881.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14875..15838
                     /locus_tag="OO315_003063"
                     /pseudo
     CDS             14875..15838
                     /locus_tag="OO315_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15883..>16082
                     /locus_tag="OO315_003064"
     CDS             15883..>16082
                     /locus_tag="OO315_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR5891882.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVG"
BASE COUNT         3974 a         4158 c         4061 g         3889 t
ORIGIN      
        1 agcagctaga ttatttttaa gaatatataa aaagaaagcg ggccgattta cgtaaataaa
       61 tcgacccgcc agcggaataa tcttaattat tcatcttcaa gataagtata accgtacaaa
      121 cctgcctcga actcttcaag gaactgttgt tgcagttcag catcaagatc ggttttcttc
      181 acttgatcgc ggaactgggt taacagcgtt ttcggatcga gctgtacata ttgcagcatg
      241 tccgccacgg tatcgccttc gtcagacagt tctacttcta cgctaccgtc agggaacacg
      301 aacacgtcaa ctgcttcggt atcaccgaac aggttgtgca tattgccaag gatctcctga
      361 tatgcaccga ccataaagaa accgagcatc ggcggattct ctggatcgta ctccggcatt
      421 ggcattgtcg tggcaatacc atcaccatca atatagtggt cgatagcacc gtcagagtca
      481 caggtaatat ccagcagcac agcgcgacgt tccggcactt gatccagccc ttccagcggt
      541 agaaccggga acaactggtc gatcccccat gcgtccggca ttgactggaa cagcgagaaa
      601 ttgacgtaca ttttgtccgc catacgttcc tgcagttcgt cgattatcgg acgatgcgca
      661 cggttttgcg gatccagttg cttctgcact tcatggcaca tgctcagata gagttgttct
      721 gcccatgcac gttcttgcag gctaaaggtg ccggaagagt agccgatatg aatgtcgtgc
      781 agatccatct ggctgtcgtg taaccattca cgcaaagaac ggcgagttcc cggttcgtgc
      841 atttcctgcc aggtttccca catgctttgc agcgcacgcg gcgcatcttc tacgggcgcg
      901 gtcggcaccg tatattcgtt acgttccacg ccgatgatat tcgacaccag cacggtatgg
      961 tgcgcagtca ccgcacggcc cgattcggtg attaccgtcg gatgcggcag accattttct
     1021 tcacacgcat cgccaatcgc ccagatgatg ttgttggcgt attcattgag gccatagttt
     1081 acggagcagt cagactgcga acgggtgcct tcataatcca cgcccagacc gccgccgacg
     1141 tcgaagcact gaatattgac gcccagcttg tgcagttcca catagaaacg cgcggattca
     1201 cgaacgcctg tcgcgatatc gcgaatattc gccatctgcg aaccgaggtg gaagtgcagt
     1261 agttgcaggc tgtcgagacg cccggcttca cgcagggttt caaccagttg cagtacctga
     1321 gtcgcagcca ggccgaactt cgatttttcc ccgccggagg actgccattt acccgagccc
     1381 tgcgaagcca gacgtgcacg cacgcccaga cgaggaacga cattcagacg ttctgcttca
     1441 tccagcacaa tggcgatttc tgacatcttc tcaatgacca gatagacctt gtgccccatc
     1501 ttctcgccaa ttaatgccag gcggatatat tcgcggtctt tataaccgtt gcagacgatg
     1561 acgctacgag tcatgccagc atgtgccagc actgccatca actcggcttt ggaaccggct
     1621 tccagaccca gcggttcacc cgaatgaatc agggactcga tcacgcggcg gtgctggtta
     1681 actttgatcg gataaacaag gaagtaatcg ccgttatagc cgtaggattc cctcgcacgt
     1741 ttgaacgcgg cgttaatgga acgcaaacgg tgctgcagga tctgtgggaa acagaacagt
     1801 gcaggcagac gctgaccctg tgcttcacga gttttcacta actgcgcgag atcgacgcga
     1861 gcttccggga cgtccgggtc cgggcacacg ctgatgtggc ccagctcgtt aacgtcatag
     1921 tagttattgc cccaccaggc aatattgtaa gtacgcagca tcttgctggc ttcctgggag
     1981 ctcattgcaa cctcctgcat ggagcgtagt acaccgtgtt cgcccgctga cgaaggcaaa
     2041 cccatagaca tgtcgtcaga catagcgaac ctcaaattat tttattaagt gtaaaacagt
     2101 taacgactgt cgcagcctgg aactgcgata acaacccata agcacatgga ttttccagca
     2161 gtgtatgctg acgctccgac tgcgcaaccg gtttcttttt cataacatta ttaagcacat
     2221 aaccgaacgt aagtgtgaca gttcggcgaa accacgagaa aactcttgtt tttacaagag
     2281 cgcccttgtt cagtcctcag taactgtaac cagctcttga atcctgagaa gcgccgagat
     2341 gggtataaca tcggcaggta tgcaaagcag agatgcagag tgcggggaac gagtcttcac
     2401 cagaacggtg agacaggtta agcagcagac aacggttcat tatttcgtat cacctccacg
     2461 gccgcctgtt aagacgaacc cacaagccaa aactctgatt tcaacccggc tggaagtggc
     2521 aacacgaaag aaacgtcgtg tgctttttat ttaagccgcg cgccgcgttt tataccccac
     2581 aatggcagaa aattgcaaaa gataaatacg cagaatgccg gcattgtcag gaaaaatttc
     2641 cagccacgtt tttaacagaa tgagacacga ttcaaaaaaa agtggaaatc gggtgaagaa
     2701 ttgacctaaa atagccatcc agatgttaat ccatccatac cgattaacac tcagactgcc
     2761 agtgttttta acctgcagag tcgtggtagg atccgctacc acagaaaatc cacacaacag
     2821 tttgagctaa ccaaattctc tttaggtgat attaaatatg gcaaaacacc tttttacgtc
     2881 cgagtccgtc tctgaagggc atcctgacaa aattgctgac caaatctctg atgccgtttt
     2941 agacgcgatc ctcgaacagg atccgaaagc acgcgttgct tgcgaaacct acgtaaaaac
     3001 cggcatggtt ttagttggcg gcgaaatcac caccagcgcc tgggtagaca tcgaagagat
     3061 cacccgtaac accgttcgcg aaattggcta tgtgcattcc gacatgggct ttgacgctaa
     3121 ctcctgtgcg gttctgagcg ctatcggcaa acagtctcct gacatcaacc aaggcgttga
     3181 ccgtgccgat ccgctggaac agggcgcggg tgaccagggt ctgatgtttg gctacgcaac
     3241 taatgaaacc gacgtgctga tgccagcacc tatcacctat gcacaccgtc tggtacagcg
     3301 tcaggctgaa gtgcgtaaaa acggcactct gccgtggctg cgcccggacg cgaaaagcca
     3361 ggtgactttc cagtatgacg acggcaaaat cgttggtatt gatgctgtcg tgctttccac
     3421 tcagcactct gaagagatcg accagaaatc gctgcaagaa gcggtaatgg aagagatcat
     3481 caagccaatt ctgcccgctg aatggctgac ttctgccacc aaattcttca tcaacccgac
     3541 cggtcgtttc gttatcggtg gcccaatggg tgactgcggt ctgactggcc gtaaaattat
     3601 cgttgatacc tacggcggca tggcgcgtca cggtggcggt gcattctctg gtaaagatcc
     3661 atcaaaagtg gaccgttccg cagcctacgc agcacgttat gtcgcgaaaa acatcgttgc
     3721 tgctggcctg gccgatcgtt gtgaaattca ggtttcctac gcaatcggcg tggctgaacc
     3781 gacctccatc atggtagaaa ctttcggtac tgagaaagtg ccttctgaac aactgactct
     3841 gctggtacgt gagttcttcg acctgcgccc atacggactg attcagatgc tggatctgct
     3901 gcacccgatc tacaaagaaa ccgcagcata cggtcacttt ggtcgtgaac atttcccgtg
     3961 ggaaaaaacc gacaaagcgc agctgctgcg cgatgctgcc ggtctgaagt aatctttctt
     4021 cacctgcgtt caaaggccag cctcgcgctg gcctttttct tttacctcac cgcggtggat
     4081 aggcgttcac gccgcatccg gcaaaaaaac tgcccgcaca ataacatcat tcttcctgat
     4141 cacgtttcac ggcaaattac catcacaact gaaaccgatt acaccaacca caccagcaaa
     4201 atatttgtaa tattttcata ttattattca gctttcacag ttgttacatt tcttttcagt
     4261 aaagtcttaa ttgcagatga cagcgtttaa tctatgatga tataactcaa ttattttcat
     4321 gcccttaaat cataactaag ataaatgtta gtgtaagcga ttacactgat gtgatttgct
     4381 tcacatcttt ttacgtcgta ctcacctatc ttaattcaca ataaaaaata actatattgg
     4441 agggcatcat gcctgacgct aaaaaacagg ggcggtcaaa caaggcaatg acgtttttcg
     4501 tctgcttcct tgccgctctg gcgggattac tctttggcct ggatatcggt gtaattgctg
     4561 gcgcactgcc gtttattgca gatgaattcc agataacttc gcacacgcaa gaatgggtcg
     4621 taagctccat gatgttcggt gcggcagtcg gtgcggtggg catcggctgg ctctccttta
     4681 aactcgggcg caaaaagagc ctgatgatcg gcgcaatcct gtttgttgcc ggttcactgt
     4741 tctctgcggc tgcgccaaac gttgaagtac tgattctttc gcgcgttctg ctggggctgg
     4801 cggtgggtgt ggcctcttat accgcaccac tgtacctgtc agaaattgcg ccggaaaaaa
     4861 ttcgcggcag tatgatctcg atgtatcagt tgatgatcac tatcgggatc ctcggtgctt
     4921 atctttctga taccgccttc agctacaccg gtgcatggcg ctggatgctg ggtgtgatta
     4981 tcatcccggc aattttgctg ctgattggtg tcttcttcct gccagacagc ccacgttggt
     5041 ttgccgccaa acgccgtttt gttgatgccg aacgcgtgct gctacgcctg cgtgacacca
     5101 gcgcggaagc gaaacgcgaa ctggatgaaa tccgtgaaag tttgcaggtt aaacagagtg
     5161 gctgggcgct gtttaaagag aatagcaact tccgccgcgc ggtgttcctt ggcgtactgt
     5221 tacaggtaat gcagcaattc accgggatga acgtcatcat gtattacgcg ccgaaaatct
     5281 tcgaactggc gggttatacc aacaccaccg agcaaatgtg ggggacagtg attgtcggcc
     5341 tgaccaacgt acttgccacc tttatcgcaa tcggccttgt tgaccgctgg ggacgtaaac
     5401 caacgctaac gctgggcttc ctggtgatgg ctgctggcat gggcgtactc ggtacaatga
     5461 tgcatatcgg tatccactct ccgtcggcgc agtatttcgc catcgccatg ctgctgatgt
     5521 ttattgtcgg ttttgccatg agtgccggtc cgctgatttg ggtactgtgc tccgaaattc
     5581 agccgctgaa aggccgcgat tttggcatca cctgctccac cgccaccaac tggattgcca
     5641 acatgatcgt tggcgcaacg ttcctgacca tgctcaacac gctgggtaac gccaacacct
     5701 tctgggtgta tgcggctctg aacgtactgt ttatcctgct gacattgtgg ctggtaccgg
     5761 aaaccaaaca cgtttcgctg gaacatattg aacgtaatct gatgaaaggt cgtaaactgc
     5821 gcgaaatcgg cgctcacgat taatctcctc aggcttcctc ccgtcgcgga ggaagccgcc
     5881 tcttgcagtc atcttttctt cgctctatcc tctgccgcta tgaaaacatc ccgtctccct
     5941 atcgccatcc aacaggccgt tatgcgccgc ctgcgggaaa aactcgccca ggccaacctg
     6001 aagctagggc gtaactaccc ggagccaaaa ctctcttaca cccagcgcgg aacctccgcc
     6061 ggaacagcct ggctggaaag ctatgaaatc cgcctcaatc ccgttttgct gttggaaaac
     6121 agtgaagctt ttattgaaga agtggtaccg cacgaactgg cacatttgct ggtatggaaa
     6181 catttcggcc gcgtagcgcc acatggcaaa gagtggaagt ggatgatgga aagcgtgctg
     6241 ggcgttcccg cccgtcgtac gcatcagttc gaactgcaat ccgtgcgtcg caacaccttc
     6301 ccctaccgct gtaagtgcca ggaacatcag cttaccgtac gccgccataa tcgcgtagtt
     6361 cgtggcgagg ccatctatcg ctgtgttcac tgcggtgaac agctggttgc gaaataacca
     6421 cctgaactat caggaacttt cctgatctgg ctgattgcat accaaaacaa ctttcgctac
     6481 gttgctggct cgttttaaca cggagtaagt gatgtaccgt tatttgtcta ttgctgcggt
     6541 ggtactgagc gcagcatttt ccggcccgac gttggccgaa ggtatcaata gtttttctca
     6601 ggcgaaagcc gcggcggtaa aagtccacgc tgatgcgccc ggtacgtttt attgcggatg
     6661 taaaattaac tggcagggca aaaaaggcgt tgttgatctg caatcgtgcg gctatcaggt
     6721 gcgcaaaaat gaaaaccgcg ccagccgcgt agagtgggaa cacgtcgttc ccgcctggca
     6781 attcggtcac cagcgccagt gctggcagga cggtggacgt aaaaactgcg ctaaagatcc
     6841 ggtctatcgc aagatggaaa gcgatatgca taacctgcag ccgtcagtcg gcgaggtgaa
     6901 tggcgatcgc ggcaatttta tgtacagcca gtggaatggc ggcgaaggcc agtacggtca
     6961 atgcgccatg aaggtcgatt tcaaagaaaa agctgccgaa ccaccagcgc gtgcacgcgg
     7021 tgccattgcg cgcacctact tctatatgcg cgaccaatac aacctgacac tctctcgcca
     7081 gcaaacgcag ctgttcaacg catggaacaa gatgtatccg gttaccgact gggagtgcga
     7141 gcgcgatgaa cgcatcgcga aggtgcaggg caatcataac ccgtatgtgc aacgcgcttg
     7201 ccaggcgcga aagagctaac ctacactagc gggattcttt ttgttaaccc ctaccccacg
     7261 cgtacaaccg cgtggggaga cgacgcggat ttttaactat gcgtatcccc cgcatttatc
     7321 atcctgaacc actgaccagc cattctcaca tcgcgctttg cgaagatgcc gccaaccata
     7381 tcgggcgcgt actgcgcatg gggccggggc aggcgttgca attgtttgac ggtagcaacc
     7441 aggtctttga cgccgaaatt accagcgcca gcaaaaaaag cgtggaagtg aaggtgctgg
     7501 aaggccagat cgacgatcgc gaatctccgc tgcatattca cctcggtcag gtgatgtcgc
     7561 gtggtgaaaa aatggaattt actatccaga aatcgatcga actcggtgta agcctcatta
     7621 cgccactttt ttctgagcgc tgcggcgtta aactggatag tgaacgtctg aacaagaagc
     7681 ttcagcagtg gcagaagatt gcaattgctg cctgtgagca gtgtggtcgt aaccgggtgc
     7741 cggaaatccg tccagcgatg gatctggaag cctggtgtgc agagcaggat gaaggactga
     7801 aactgaatct tcacccgcgc gccagtaaca gcatcaatac gttgccttta ccggttgaac
     7861 gcgtccgcct gctgattggc ccggaaggcg gtttatcggc agatgaaatt gccatgactg
     7921 cccgctatca atttactgat atcctgttgg gacctcgcgt tttgcgtaca gagacaactg
     7981 cgctcaccgc cattaccgcg ctacaagtac gatttggcga tttgggctaa cggagaagaa
     8041 taatgatcaa gctcggcatc gtgatggacc ccatcgcaaa catcaacatc aagaaagatt
     8101 ccagtttcgc tatgttgctg gaagcacagc gtcgtggtta cgaacttcac tatatggaga
     8161 tgggcgatct gtatctgatc aatggcgaag cccgcgccca tacccgcacg ctgaacgtga
     8221 agcagaacta cgaagagtgg ttttcgttcg tcggtgaaca ggatctgccg ctggccgatc
     8281 tcgatgtgat cctgatgcgt aaagacccgc cgtttgatac cgagtttatc tacgcgacct
     8341 atattctgga acgtgccgaa gagaaaggga cgctgatcgt taacaagccg cagagcctgc
     8401 gcgactgtaa cgagaaactg tttaccgcct ggttctctga cttaacgcca gaaacgctgg
     8461 ttacgcgcaa taaagcgcag ctaaaagcgt tctgggagaa acacagcgac atcattctta
     8521 agccgctgga cggtatgggc ggcgcgtcga ttttccgcgt gaaagaaggc gatccaaacc
     8581 tcggcgtgat tgccgaaacc ctgactgagc atggcacttg ctactgcatg gcgcaaaatt
     8641 acctgccagc cattaaagat ggcgacaaac gcgtgctggt ggtggatggc gagccggtac
     8701 cgtactgcct ggcgcgtatt ccgcaggggg gcgaaacccg tggcaatctg gctgccggtg
     8761 gtcgcggtga acctcgtccg ctgacggaaa gtgactggaa aatcgcccgt cagatcgggc
     8821 cgacgctgaa agaaaaaggg ctgatttttg ttggtctgga tatcatcggc gaccgtctga
     8881 ctgaaattaa cgtcaccagc ccaacctgta ttcgtgagat tgaagcagag tttccggtgt
     8941 cgatcaccgg aatgttaatg gatgccatcg aagcacgttt acagcagcag taacccacct
     9001 tagcgagaag gatctcgttg agactctgag tgacagcgcc cttctttcca cgcatactgg
     9061 gcgctgttgc ttttttgaac caggaaacag aacctctgac aatgaattta cagcatcact
     9121 ttcttattgc catgcctgct ctccaggatc cgattttccg tcgttccgtg gtctacattt
     9181 gcgaacataa taccaatggt gcaatgggga tcatcgtcaa caagccgctg gaaaatctca
     9241 aaattgaagg gattctggaa aagctgaaga tcacgccgga gccgcgtgat gaatcaatcc
     9301 gtctggataa accggttatg ctcggcggcc cactggctga agatcgcggg tttattttgc
     9361 atactccgcc ctccaatttt gcttccagca ttcgcatttc agacaacacg gtgatgacca
     9421 cttcccgcga tgtgctggaa acgctcggca ccgataaaca accgtctgac gtattggtgg
     9481 ctctgggcta tgcctcctgg gaaaaaggtc aactggaaca agaaattctc gataacgcgt
     9541 ggctaacggc cccggcggat ctgaatattc tgttcaaaac gccgattgcc gaccgctggc
     9601 gcgaggcggc aaaactgatt ggtgtggata ttctcaccat gcctggtgtg gcaggacacg
     9661 cctgatgagt ggaaccttac tcgccttcga cttcggcacc aaaagcattg gcgtagcggt
     9721 cggccaacgc attaccggca ccgctcgccc tttgcctgca attaaagcgc aggacggtac
     9781 gccggactgg aacctcatcg aacgtttact gaaagagtgg cagcctgacg aaatcatcgt
     9841 cggtttgccg ctgaatatgg acggcaccga acagccgtta accgccagag cgcgcaaatt
     9901 tgccaaccgt attcatggtc gtttcggtgt tgaagtaaag ctccatgacg agcgtcttag
     9961 cactgtggaa gcccgttccg gtctttttga acagggcggc tatcgggcgc tcaacaaagg
    10021 caaagttgac tctgcctctg cggttattat tctcgaaagc tatttcgagc agggatatta
    10081 aggcgatcta aaagcctggc ggatagtcgt taccgccatc ttgccggatg cggcgtgaac
    10141 gccttatcca gcacacatct ggcagcggct gtaggtctga taagacgcgt tagcgtcgca
    10201 tcagacattt attgccttta taggccctat aaacttgcgc atcgggcatg gcaacgtcac
    10261 aaacgccctt cccccacccg ctgctgataa ctctgctgaa acgttaacat ccccaccttc
    10321 tgcccggttt gaataacatg cggtaactgg tgggttttcc cttcgcgaat caaattcccc
    10381 accgcgggag tgttaatcag taattcaaac agcgccacgc gtccttcctg tttatccact
    10441 tccagctttt gtgacagcac tgcccgtaaa ctacccgcaa gttgattacg tacgggatct
    10501 ttttcctgcg ccggaaatga atccaccagt cgctcaactg cctgcgcggc accacgtgta
    10561 tgtaaggttg ccagcaccag atgcccggtt tctgccgccg tcagcgccag acggattgtc
    10621 tcactgtcac gtagctctcc gagcaaaatc acatcaggat cttcccgcaa tgcagcccgc
    10681 aaccccgatg cgaacgtcat acagtgcaaa ccaatttccc gctgctggat caaacatcgc
    10741 tggctggcat agagatattc cacaggatct tccagcgtca gaatatgcgc atcggcatgt
    10801 tgattaagat agccaaccat cgccgccagc gtggtagatt tgccactccc cgtagccccc
    10861 gtcaccagaa tcaggccatt ctcgctcttg agtaattccg gcaataccgg tggtgcacca
    10921 agctgttcga gctgtggaca gtgcgaaggt aacaaccgta atgccagaga aataccttgc
    10981 cgttgcgcga acgcactgcc acgcaaccgc tggttttccg ccagcgacac ggcaaaatcc
    11041 agctggccat tttccagcaa tattgcccgc tgatcgtcat ccagccactc ccgcagtagc
    11101 tcttcgacgt ccggcgcatc aaacggcgca gcttccattc tgccacgaat gcgccatcgt
    11161 gcgggccagg cgctgcacag gtgtagatcc gagacgttat gctttacact aagggccaca
    11221 atttcttcca tattcatact aagatcctcg gaaaatgaac gatattgcgc ataacctggc
    11281 acaggtccgg gacaaaatct cagcggctgc aacgcgttgc ggccgttctc cagaagaaat
    11341 tacgctgctt gcagtcagta aaacaaaacc tgcgagcgcc atcgcagaag ccattgatgc
    11401 cgggcagcgt caatttggtg aaaactacgt tcaggaaggg gtagataaaa ttcgccactt
    11461 tcaggaactg ggcgtaacag gattagaatg gcattttatt ggcccgttgc agtctaataa
    11521 aagccgccta gtggcagagc atttcgactg gtgtcatacc atcgaccgtt tgcgcattgc
    11581 tacccgcctc aacgagcagc gcccggcaga acttccccct cttaacgtac tgattcaaat
    11641 taatattagt gatgaaaaca gtaagtccgg gattcaactg gcagaactgg acgagctggc
    11701 agcggcggtc gctgaactac cgcgtttacg tctgcgcggg ctgatggcaa tccctgcgct
    11761 tgagtcagaa tatgtaaggc agtttgaagt tgcacgccaa atggctgtag catttgccgg
    11821 actgaaaacg cgctacccgc atatcgacac gctctctctg ggaatgtcgg acgatatgga
    11881 agccgccatt gcggcaggta gcacgatggt tcgtatcggc actgcaattt ttggtgcgcg
    11941 tgattactct aaaaaataag gaattaaagg aacgccatga atacgttgac tttcctgctt
    12001 tcaacggtca ttgagctgta tacaatggtg ctgttattac gcatctggat gcagtgtgct
    12061 cattgtgatt tttacacccc cttctcacag tttgtagtga aggtagcgca gccaattatc
    12121 gggccactgc gccgcgttat tccggcaatg ggtcccattg acagcgcctc gctgctggtt
    12181 gcctatattc tcagttttat caaagccatt gtgctgtttg aagtggtgac cttcctgcca
    12241 atcatctgga ttgccggttt actgattctg ctgaaaacca tcggcttgct gattttctgg
    12301 gtcctgctgg tgatggcgat tatgagctgg gtaagccagg gtcgtagccc gattgaatac
    12361 gtgctgattc agctggccga tccgctgctg cgcccgattc gccgcttgct accggcaatg
    12421 ggtgggattg atttctcgcc gatgatcctc gttctgctgc tgtatgccat caatatgggt
    12481 gtcgcagaag tattacaggc aaccggaaac atgctgctgc cggggctgtg gatggcgtta
    12541 tgagtgccgt aacagttaat gatgacggtc tggttttacg gctctatatt cagccgaaag
    12601 ccagccgtga ttctattgtc ggtttacatg gcgacgaagt taaagtcgcc attaccgcgc
    12661 cgccggttga cggccaggcc aacagtcatc tggtgaagtt tctcggtaag caattccggg
    12721 ttgccaaaag ccaggtggtg attgaaaaag gcgaacttgg ccgccacaaa caaattaaaa
    12781 tcattaatcc gcaacaaatc ccgccagaaa tcgcggcgtt aattaattag gtatcctatg
    12841 caaaaagttg tcctcgcaac cggcaatgcc ggtaaagtgc gtgagctggc gtcgctgctt
    12901 agcgactttg gtcttgatat cgtggcccaa acagacctcg gcgttgattc cgctgaagaa
    12961 accggcctaa cctttatcga aaacgcgatt ctgaaagcgc gccatgcggc aaaagtgacc
    13021 ggtttaccgg caattgccga cgactctggt ctggcggtag atgcgctggg tggcgcgccg
    13081 ggtatttact ccgcgcgtta ttccggtgaa gacgcgaccg atcaaaagaa tctgcaaaaa
    13141 ctgttggaaa cactgaaaga cgtaccggac gaccaacgtc aggcgcgttt ccactgcgtg
    13201 ctggtatatc tgcgtcacgc ggaagatccg actccgctgg tatgccacgg cagctggccg
    13261 ggcgtgatta ctcgcgaacc agcgggcact ggtggctttg gttatgatcc aatcttcttc
    13321 gtaccttccg aagggaaaac cgccgccgaa ctgacccgcg aagaaaagag cgccatttcc
    13381 caccgtggtc aggcgttgaa actgctgctg gacgctttac gtaatggtta aattaccgcc
    13441 gctgagtctc tacattcaca tcccgtggtg cgtgcagaaa tgcccgtact gcgatttcaa
    13501 ctctcacgcg ttgaaaggag aagtgccgca cgacgattat gttcagcatc tgctttgcga
    13561 tctggacaat gatgtggctt acgctcaggg ccgtgaagta aagacaatct ttattggcgg
    13621 tggtacgccg agcctgcttt ccggcccggc gatgcaaacg ctgctagacg gcgtgcgtgc
    13681 gcgtttgccg ctggcagcgg atgcagaaat tactatggaa gcgaacccag gcacggtaga
    13741 agcggatcgc tttgtcgatt atcagcgtgc tggtgtaaac cgcatctcta ttggtgtgca
    13801 gagttttagc gaagaaaagc tgaaacgact tgggcgtatt catggcccgc aagaagcgaa
    13861 acgagcggca aagctggcga gcggtttagg gttacgtagc tttaaccttg atttgatgca
    13921 tggactaccg gatcaatcac tggaagaggc gcttggcgat ctacgccagg ccattgaact
    13981 gaatccgccg catctttcct ggtatcaact gactatcgaa cctaatacgc tgtttggctc
    14041 gcgaccgccg gtgctgccgg acgatgacgc gctgtgggat attttcgaac aggggcatca
    14101 gttattaacc gcagcgggtt atcagcaata tgaaacttcc gcttacgcca aacccggtta
    14161 tcagtgccag cacaatctca actactggcg ctttggtgac tacatcggta ttggctgcgg
    14221 cgcgcacggc aaagtgacct tcccggatgg gcgcattctg cgtaccacta aaacgcgtca
    14281 tcctcgtggt tttatgcaag gaaggtatct ggaaagccag cgtgatgtcg aagccgcaga
    14341 taagccgttt gagttcttta tgaatcgctt ccgtctgctg gaggccgcgc cgcgcgtgga
    14401 gtttattgcg tataccgggc tttgcgaaga tgtgattcgc ccacagttag acgaggcgat
    14461 tgcccagggt tatctcaccg aatgtgcgga ttactggcag ataacggaac atggtaaatt
    14521 attcttaaac tcacttcttg aattattcct cgctgagtaa tcattaatgc gcctgcgggc
    14581 gcattaatca cttcttttat ccccccgtta attaacctcg gcagaaatat atttcttact
    14641 gaataaatat cgacgacatt tattatatat atttagtgaa tatttactat atcattcatt
    14701 gattataaat atgtacaacc tggcgcacaa atttaatatt taatttctga ctttatttca
    14761 agttgtgata aacatagaca agttgtcaga caggttaacc aatttgtcgc acaaatcact
    14821 gataagcaca attagatatc aaccaatttc catccttcaa cagggagttt ttttatgaaa
    14881 atgaggtcat ttacccgctc attagtctgc gcatccttgc tggctctggt tagcactggc
    14941 gtaaacgccg cagaaaaagt ggcgttaaag ttagcacaca acctggaaag aagtcatgtt
    15001 gtacatcaga gttttgaaga gctggcgaaa gaggtaaaac agctgtcaaa aggtaatatg
    15061 gtgatccgta tttatcccag cagccagatg ggtaatgcgc gagaaactat ggaattgttg
    15121 caaaatggcg cactggatat gaccaaaggt tccgccagtg atctggagtc ttttgataat
    15181 atttatgcta tctataattt gccgttctta ttaagttgtt tttggtgaag ttggtaaaga
    15241 aattatggac tcaacaaaag acaaaggctt ttttgcttta tcagcttatg tagcgggtac
    15301 tcgcagtttc tatgcgaaaa aaccgattac aaagccagaa gatcttaaag gactgaaaat
    15361 tcgtgttcag ccaagtccga ccaccatcaa aatgattgaa ctgatgggtg gttcacccac
    15421 tccaatttca tttggcgaag tttatactgc gatgcagcaa ggtgtagtag atggcgcaga
    15481 aaataacgtg ccttcctgga tgcagactcg acatattgaa attgccaaag ttttttctga
    15541 agatgaacac gcctcaattc ctgacttttt ggttatctcc accaaaacat ggaataaatt
    15601 aaccccagaa cagcaacaaa tcctcgaaac agcggcgaag aaatccgaag cttatcaaca
    15661 aaaattgtgg gaaaaaattg acgctgacac acgtgctcag gcgaaagcta tggggggtga
    15721 aattgtaaaa gtggataaag cgccattccg taaagccgtg caacctctgt ttgacgactt
    15781 caaaaaagat ccaaaacaag ctgctttgtt agagaaattc gacaacgctg ctcaataata
    15841 ttccgggcca gcctctggcc ctgattttcc aggatgctta caatgatatt taaccgcctg
    15901 aaacttgcgg tagaccgcgt cattgccacg ttttctgtcg ccgtaatgct tgctctggtt
    15961 gtatgcgtag tctggcaagt atttagccgc tacgtactta accagccaag tacattaacc
    16021 gatgagctgg cacgcttttt gatgatctgg gtaggattat taggtgctgc atataccgtc
    16081 gg
//
LOCUS       DAJTRD010000086        15803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTRD010000086 DAJTRD010000000
VERSION     DAJTRD010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.086"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..295
                     /gene="tuf"
                     /locus_tag="OO315_003065"
     CDS             <1..295
                     /gene="tuf"
                     /locus_tag="OO315_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5891883.1"
                     /translation="TIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGT
                     IELPEGVEMVMPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            478..672
                     /gene="bfd"
                     /locus_tag="OO315_003066"
     CDS             478..672
                     /gene="bfd"
                     /locus_tag="OO315_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5891884.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            745..1221
                     /gene="bfr"
                     /locus_tag="OO315_003067"
     CDS             745..1221
                     /gene="bfr"
                     /locus_tag="OO315_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5891885.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1218..1685)
                     /locus_tag="OO315_003068"
                     /pseudo
     CDS             complement(1218..1685)
                     /locus_tag="OO315_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            2064..2375
                     /gene="rpsJ"
                     /locus_tag="OO315_003069"
     CDS             2064..2375
                     /gene="rpsJ"
                     /locus_tag="OO315_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5891886.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2408..3037
                     /gene="rplC"
                     /locus_tag="OO315_003070"
     CDS             2408..3037
                     /gene="rplC"
                     /locus_tag="OO315_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5891887.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3048..3653
                     /gene="rplD"
                     /locus_tag="OO315_003071"
     CDS             3048..3653
                     /gene="rplD"
                     /locus_tag="OO315_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5891888.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3650..3952
                     /gene="rplW"
                     /locus_tag="OO315_003072"
     CDS             3650..3952
                     /gene="rplW"
                     /locus_tag="OO315_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5891889.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3970..4791
                     /gene="rplB"
                     /locus_tag="OO315_003073"
     CDS             3970..4791
                     /gene="rplB"
                     /locus_tag="OO315_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5891890.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4808..5086
                     /gene="rpsS"
                     /locus_tag="OO315_003074"
     CDS             4808..5086
                     /gene="rpsS"
                     /locus_tag="OO315_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5891891.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5101..5433
                     /gene="rplV"
                     /locus_tag="OO315_003075"
     CDS             5101..5433
                     /gene="rplV"
                     /locus_tag="OO315_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5891892.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5451..6152
                     /gene="rpsC"
                     /locus_tag="OO315_003076"
     CDS             5451..6152
                     /gene="rpsC"
                     /locus_tag="OO315_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5891893.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6165..6575
                     /gene="rplP"
                     /locus_tag="OO315_003077"
     CDS             6165..6575
                     /gene="rplP"
                     /locus_tag="OO315_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5891894.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6575..6766
                     /gene="rpmC"
                     /locus_tag="OO315_003078"
     CDS             6575..6766
                     /gene="rpmC"
                     /locus_tag="OO315_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5891895.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6766..7020
                     /gene="rpsQ"
                     /locus_tag="OO315_003079"
     CDS             6766..7020
                     /gene="rpsQ"
                     /locus_tag="OO315_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5891896.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7185..7556
                     /gene="rplN"
                     /locus_tag="OO315_003080"
     CDS             7185..7556
                     /gene="rplN"
                     /locus_tag="OO315_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5891897.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7567..7881
                     /gene="rplX"
                     /locus_tag="OO315_003081"
     CDS             7567..7881
                     /gene="rplX"
                     /locus_tag="OO315_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5891898.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7896..8435
                     /gene="rplE"
                     /locus_tag="OO315_003082"
     CDS             7896..8435
                     /gene="rplE"
                     /locus_tag="OO315_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5891899.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8450..8755
                     /gene="rpsN"
                     /locus_tag="OO315_003083"
     CDS             8450..8755
                     /gene="rpsN"
                     /locus_tag="OO315_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5891900.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8789..9181
                     /gene="rpsH"
                     /locus_tag="OO315_003084"
     CDS             8789..9181
                     /gene="rpsH"
                     /locus_tag="OO315_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5891901.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9194..9727
                     /gene="rplF"
                     /locus_tag="OO315_003085"
     CDS             9194..9727
                     /gene="rplF"
                     /locus_tag="OO315_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5891902.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9737..10090
                     /gene="rplR"
                     /locus_tag="OO315_003086"
     CDS             9737..10090
                     /gene="rplR"
                     /locus_tag="OO315_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5891903.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10105..10608
                     /gene="rpsE"
                     /locus_tag="OO315_003087"
     CDS             10105..10608
                     /gene="rpsE"
                     /locus_tag="OO315_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5891904.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10612..10791
                     /gene="rpmD"
                     /locus_tag="OO315_003088"
     CDS             10612..10791
                     /gene="rpmD"
                     /locus_tag="OO315_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5891905.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10795..11229
                     /gene="rplO"
                     /locus_tag="OO315_003089"
     CDS             10795..11229
                     /gene="rplO"
                     /locus_tag="OO315_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5891906.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11237..12568
                     /gene="secY"
                     /locus_tag="OO315_003090"
     CDS             11237..12568
                     /gene="secY"
                     /locus_tag="OO315_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5891907.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12600..12716
                     /gene="rpmJ"
                     /locus_tag="OO315_003091"
     CDS             12600..12716
                     /gene="rpmJ"
                     /locus_tag="OO315_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5891908.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12863..13219
                     /gene="rpsM"
                     /locus_tag="OO315_003092"
     CDS             12863..13219
                     /gene="rpsM"
                     /locus_tag="OO315_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5891909.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13236..13625
                     /gene="rpsK"
                     /locus_tag="OO315_003093"
     CDS             13236..13625
                     /gene="rpsK"
                     /locus_tag="OO315_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5891910.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13659..14279
                     /gene="rpsD"
                     /locus_tag="OO315_003094"
     CDS             13659..14279
                     /gene="rpsD"
                     /locus_tag="OO315_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5891911.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14305..15294
                     /gene="rpoA"
                     /locus_tag="OO315_003095"
     CDS             14305..15294
                     /gene="rpoA"
                     /locus_tag="OO315_003095"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5891912.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15335..15718
                     /gene="rplQ"
                     /locus_tag="OO315_003096"
     CDS             15335..15718
                     /gene="rplQ"
                     /locus_tag="OO315_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5891913.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
BASE COUNT         4070 a         3721 c         4204 g         3808 t
ORIGIN      
        1 caccatcaag ccgcacacca agttcgaatc tgaagtgtac attctgtcca aagatgaagg
       61 cggccgtcat actccgttct tcaaaggcta ccgtccgcag ttctacttcc gtactactga
      121 cgtgactggt accatcgaac tgccggaagg cgtagagatg gtaatgccgg gcgacaacat
      181 caaaatggtt gttaccctga tccacccgat cgcgatggac gacggtctgc gtttcgcaat
      241 ccgtgaaggc ggccgtaccg ttggcgcggg cgttgttgct aaagttctgg gctaattaca
      301 cgttaattag ttttgaattg aaaagggcgc ttcggcgccc tttttgctgc ccgtaatttg
      361 agctcgaaat atttagtcgt aattttgctc gctgaactat ggcgcaaata aaactgtgct
      421 attgtaatca taaccattct catttacact ttgtgcagaa tttgaacggg agcaatcatg
      481 tacgtttgtt tgtgtaatgg tgtaagtgac aaaaaaattc gccaggcagt ccgtcagttt
      541 catccgcaat ctttccaaca attgcgtaag tttattcctg tgggaaatca atgcggtaag
      601 tgcattcgcg ccgcgcgcga agtgatgcag gacgagttga tgcagatgcc agaatttaaa
      661 gagattgcct gaagcccacg cttatttttt gacatcctgc tagccccatc tacgctctaa
      721 tagtggaagc ggagggacta taaaatgaaa ggtgatgtta aaatcataaa ttatctcaat
      781 aaactattgg gaaatgagct tgtcgcaatc aatcagtatt ttctccatgc ccggatgttt
      841 aaaaactggg gcctgatgcg tctcaatgac gtcgaatacc atgaatccat cgatgagatg
      901 aaacacgctg ataaatatat cgagcgtatt ttgtttttag aaggaatccc caacttacag
      961 gatttaggca aactggggat tggtgaagat gtcgaggaga tgctgcaatc tgacctgcga
     1021 ttagaactgg aaggcgcgaa ggatctgcgt gaggctatcg catatgccga cagcgttcat
     1081 gactatgtta gccgcgatat gatgatcgag atccttgctg atgaagaagg tcatatcgac
     1141 tggctcgaaa ctgaactgga tctcatcggt aaaatcggcc tacaaaatta cctgcaatca
     1201 caaattaaag ttaaagatta acggcctatc agcaaactat cccagcctat aacgaaaccc
     1261 gcagccgcca gaaatggccc aaaaggcagc gggtttttta atgattcttt tcctctcatg
     1321 agcagaccaa taactacggc tccgcaagcg aatgaggcag caaggaaaac cagtcgtggt
     1381 aagaaggccc atgaatgcta ggcgccaagg gcggcgagaa acttcacatc accatagcct
     1441 aatccttctt tatgtcgcag tatgcgatac ccccagtaaa tgacagcgaa tgtgccgtaa
     1501 ccaacaattg ccccccataa cgcatcggct aaaccatcag gatgacaaac ttgatagaac
     1561 aaaagacctg accagagtaa cgggcacgta aatctgtcag gtaacaagcc atgctttgca
     1621 tcccagaaaa aaagtagggc tgataaacaa gcgtacaaaa ttagaaaggg aagggtagtg
     1681 tccataaaac atcctctgta attggagtat caccatcgaa taactccgga tgctcagcaa
     1741 cagagagagc gctgatttgt taacctgatt ccggtgaaaa acgctatctt cacaacctta
     1801 caaatttcgt ggaatgcagc tcgcaatctg cgcgttgcga ttaatttcta cccgatgctt
     1861 gcgtcaggct cagatttgcg tataatgcgc gggcttgtcg tagttgacag caggttcaat
     1921 ctgaacctca gttagccgaa tttggctacc taacaatgct cccaatcggg gagctacgta
     1981 agaacggtta cactctccca tcaatcgtaa tgggtctgag gagtaatcat tttcgtttat
     2041 aaaataattg gagctctggt ctcatgcaga accaaagaat ccgtatccgc ctgaaagcgt
     2101 ttgatcatcg tctgatcgat caagcaaccg cggaaatcgt cgagactgcc aagcgcactg
     2161 gtgcgcaggt ccgtggtccg atcccgctgc cgacacgcaa agagcgcttc actgttctga
     2221 tctccccgca cgtcaacaaa gacgcgcgcg atcagtacga aatccgtact cacttgcgtc
     2281 tggttgacat cgttgagcca accgagaaaa ccgttgatgc tctgatgcgt ctggatctgg
     2341 ctgccggtgt agacgtgcag atcagcctgg gttaatcagg tcattgagcg attgagaggt
     2401 tgaaacaatg attggtttag tcggtaaaaa agtgggtatg acccgtatct tcacagaaga
     2461 cggcgtttct atcccagtaa ccgtaatcga agttgaagca aaccgcgtta ctcaggttaa
     2521 agacctggct aacgatggct accgtgctat tcaggtgacc accggtgcta aaaaagctaa
     2581 ccgtgtgacc aagcctgaag ctggccactt cgctaaagct ggcgtagaag ctggccgtgg
     2641 tctgtgggaa ttccgcctgg ctgaaggcga agagttcact gtaggtcaga gcattagcgt
     2701 tgaactgttt gctgacgtta aaaaagttga cgtaactggc acctctaaag gtaaaggttt
     2761 cgcaggtacc gttaagcgct ggaacttccg tacccaggac gctactcacg gtaactcctt
     2821 gtctcaccgc gttccgggtt ctatcggtca gaaccagact ccgggcaaag tgttcaaagg
     2881 caagaaaatg gcaggtcaga tgggtaacga acgtgtaacc gttcagagcc ttgacgtagt
     2941 acgcgttgac gctgagcgca acctgctgct ggttaaaggt gctgtcccgg gtgcaaccgg
     3001 tagcgacctg atcgttaaac cagctgtgaa ggcgtaagga gatagcaatg gaattagtat
     3061 tgaaagacgc gcagagcgcg ctgactgttt ccgaaactac cttcggtcgt gatttcaacg
     3121 aagcgctggt tcaccaggtt gttgttgctt atgcagctgg tgctcgtcag ggtactcgtg
     3181 ctcagaagac tcgtgctgaa gtaactggtt ccggtaaaaa accgtggcgc cagaaaggca
     3241 ccggccgtgc gcgttctggt tctatcaaga gcccgatctg gcgttctggt ggcgtgacct
     3301 ttgctgctcg tccgcaggac cacagtcaaa aagttaacaa gaagatgtac cgcggcgcgc
     3361 tgaaaagcat cctgtccgaa ctggtacgtc aggatcgtct gatcgttgtc gagaagttct
     3421 ctgtagaagc gccgaaaact aagctgctgg cacagaaact gaaagacatg gctctggaag
     3481 atgtgctgat catcaccggt gagctggacg aaaacctgtt cctggctgcg cgcaacctgc
     3541 agaaggttga cgtacgcgat gcaactggta tcgacccggt tagcctgatc gccttcgaca
     3601 aagtcgtaat gactgctgat gctgttaagc aagttgagga gatgctggca tgattcgtga
     3661 agaacgtctg ctgaaggtgc tgcgtgcacc gcacgtttct gaaaaagcgt ctactgcgat
     3721 ggaaaaatcc aacaccatcg tactcaaagt tgctaaagac gcgaccaaag cagaaatcaa
     3781 agctgctgtg cagaaactgt ttgaagtcga agtcgaagtc gttaacaccc tggtagttaa
     3841 agggaaagtt aaacgtcacg gacagcgtat cggtcgtcgt agcgactgga aaaaagctta
     3901 cgtcaccctg aaagaaggcc agaatctgga cttcgttggc ggcgctgagt aagtcggagg
     3961 agtaatacaa tggcagttgt taaatgtaaa ccgacatctc cgggtcgtcg ccacgtagtt
     4021 aaagtggtta accctgagct gcacaagggc aaaccttttg ctccgttgct ggaaaaaaac
     4081 agcaaatccg gtggtcgtaa caacaatggc cgtatcacca ctcgtcatat cggtggtggc
     4141 cacaagcagg cttaccgtat tgttgacttc aaacgcaaca aagacggtat cccggcagtt
     4201 gttgaacgtc ttgagtacga tccgaaccgt tccgcgaaca tcgcgctggt tctgtacaaa
     4261 gacggtgaac gccgttacat cctggcccct aaaggcctga aagctggcga ccagattcag
     4321 tctggcgttg atgctgcaat caaaccaggt aacaccctgc cgatgcgcaa catcccggtt
     4381 ggttctactg ttcataacgt agaaatgaaa ccaggtaaag gcggtcagct ggcacgttcc
     4441 gctggtactt acgttcagat cgttgctcgt gatggtgctt atgtcaccct gcgtctgcgt
     4501 tctggtgaaa tgcgtaaagt agaagcagac tgccgtgcaa ctctgggcga agttggcaat
     4561 gctgagcata tgctgcgcgt tctgggtaaa gcaggtgctg cacgctggcg tggtgttcgt
     4621 ccgaccgttc gcggtaccgc gatgaacccg gtagaccacc cacatggtgg tggtgaaggt
     4681 cgtaactttg gtaagcaccc ggtaactccg tggggcgttc agaccaaagg taagaagacc
     4741 cgcagcaaca agcgtactga taaattcatc gtacgtcgcc gtagcaaata attttagagg
     4801 ataagccatg ccacgttctc tcaagaaagg tccttttatt gacctgcact tgctgaagaa
     4861 ggtagagaaa gcggtggaaa gcggagacaa gaagcccctg cgcacttggt cccgtcgttc
     4921 aacgatcttt cctaacatga tcggtttgac catcgctgtc cataatggtc gtcagcacgt
     4981 tccggtattt gtaaccgacg aaatggtcgg tcacaaactg ggtgaattcg caccgactcg
     5041 tacttatcgc ggccacgctg ctgataaaaa agcgaagaag aaataaggta ggaggaagag
     5101 atggaaacta ttgctaaaca tcgccatgct cgttcttctg ctcagaaggt tcgccttgtt
     5161 gctgacctga ttcgcggtaa gaaagtgtcg caggctctgg atattttgac ctacaccaac
     5221 aagaaagcgg ctgtacaggt caagaaagtt ctggaatctg ccattgctaa cgctgaacac
     5281 aacgatggcg ctgacattga cgatctgaaa gttacgaaaa ttttcgtaga cgaaggcccg
     5341 agcatgaagc gcattatgcc gcgtgcaaaa ggtcgtgcag atcgcatcct gaagcgcacc
     5401 agccacatca ctgtggttgt gtccgatcgc tgagactctg gagactagca atgggtcaga
     5461 aagtacatcc taatggtatt cgcttgggta ttgtaaaacc atggaactct acttggtttg
     5521 cgaacaccaa agaattcgct gacaacctgg acagcgattt taaagtacgt cagtacctga
     5581 ctaaggaact ggctaaagcg tccgtatctc gtatcgttat cgagcgtccg gctaagagca
     5641 tccgtgtaac cattcacact gctcgcccgg gtatcgttat cggtaaaaaa ggtgaagacg
     5701 tagaaaaact gcgtaaggtc gtagcggaca tcgctggcgt tcctgcacag atcaacatcg
     5761 ccgaagttcg taagcctgaa ctggacgcaa aactggttgc tgacagcatc acttctcagc
     5821 tggaacgtcg cgttatgttc cgtcgtgcta tgaagcgtgc tgtacagaac gcaatgcgtc
     5881 tgggcgctaa aggtattaaa gttgaagtta gcggccgtct gggcggcgcg gaaatcgcac
     5941 gtaccgaatg gtaccgcgaa ggtcgcgtac cgctgcacac tctgcgtgct gacatcgact
     6001 acaacacctc tgaagcgcac accacttacg gtgtaatcgg cgttaaagtg tggatcttca
     6061 aaggcgagat cctgggtggt atggctgctg ttgaacaacc ggaaaaaccg gctgctcagc
     6121 ctaaaaagca gcagcgtaaa ggccgtaaat aaggagcgtc gctgatgtta caaccaaagc
     6181 gtacaaaatt ccgtaaaatg cacaaaggcc gtaaccgcgg tctggcgcag ggtacggatg
     6241 ttagcttcgg cagcttcggt ctgaaagctg ttggccgtgg tcgtctgact gcccgtcaga
     6301 tcgaagcagc acgtcgtgct atgacccgtg cagttaagcg tcaaggtaag atctggatcc
     6361 gtgtgttccc ggacaaaccg atcactgaaa agccgctggc agtgcgtatg ggtaaaggta
     6421 aaggtaacgt ggagtattgg gttgccttga ttcagccggg taaagtcctg tatgaaatgg
     6481 acggtgttcc ggaagagctg gcccgtgaag cattcaagct ggcagcagcg aaactgccga
     6541 ttaaaaccac ctttgtaact aagacggtga tgtaatgaaa gcaaaagagc tgcgtgagaa
     6601 gagcattgaa gagctgaaca ccgagctgct gaacctgctg cgtgagcagt tcaacctgcg
     6661 tatgcaggct gcaagtggcc agctgcaaca gtctcacctg ttgaagcaag tgcgtcgcga
     6721 tgtcgcacgc gttaagactt tactgaacga gaaggcgggt gcgtaatgac cgataaaatc
     6781 cgtactctgc aaggtcgcgt tgttagcgac aaaatggaga aatccattgt tgttgctatc
     6841 gaacgttttg tgaaacaccc gatctacgga aaattcatca agcgtacgac caaactgcac
     6901 gtacatgacg agaacaacga atgcggtatc ggtgacgtgg ttgaaatccg cgaatgccgt
     6961 ccgctgtcca agactaaatc ctggacgctg gttcgcgttg tagagaaagc ggttctgtaa
     7021 tacagtacac tctctcgata cgaataaacg gctcagaaat gagccgttta ttttttctac
     7081 ccatatcctt gaagcggtgt tataatgccg cgccctcgat atggggattt ttaacgacct
     7141 gattttcggg tctcagtagt agttgacatt agcggagcac taaaatgatc caagaacaga
     7201 ctatgctgaa cgtcgccgac aactccggtg cacgtcgcgt aatgtgtatc aaggttctgg
     7261 gtggctcgca ccgtcgctac gcaggcgtag gcgacatcat caagatcacc atcaaagaag
     7321 caattccgcg tggtaaggtc aaaaaaggtg atgtgctgaa ggcggtagtg gtgcgcacca
     7381 agaagggtgt tcgtcgcccg gacggttctg tcattcgctt cgatggtaat gcttgtgttc
     7441 ttctgaacaa caacagcgag cagcctatcg gtacgcgtat ttttgggccg gtaactcgtg
     7501 agcttcgtag tgagaagttc atgaaaatta tctctctggc accagaagta ctctaaggag
     7561 cgaatcatgg cagcgaaaat ccgtcgtgat gacgaagtta tcgtgttaac cggtaaagat
     7621 aaaggtaaac gcggtaaagt taagaatgtc ctgtcttccg gcaaggtcat tattgaaggt
     7681 atcaacctgg ttaagaaaca tcagaagccg gttccggccc tgaaccaacc gggtggcatc
     7741 gttgaaaaag aagccgctat tcaggtttcc aacgtagcaa tcttcaatgc ggcaaccggc
     7801 aaggctgacc gtgtaggctt tagattcgaa gacggcaaaa aagtccgttt cttcaagtct
     7861 aacagcgaaa ctatcaagta atttggagta gtacgatggc gaaactgcat gattactaca
     7921 aagacgaagt agttaaaaaa ctcatgactg agtttaacta caattctgtc atgcaagtcc
     7981 ctcgggtcga gaagatcacc ctgaacatgg gtgttggtga agcgatcgct gacaaaaaac
     8041 tgctggataa cgcagcagca gacctggcag caatctccgg tcaaaaaccg ctgatcacca
     8101 aagcacgcaa atctgttgca ggcttcaaaa tccgtcaggg ctatccgatc ggctgtaaag
     8161 taactctgcg tggcgaacgc atgtgggagt tctttgagcg cctgatcact attgctgtac
     8221 ctcgtatccg tgacttccgt ggcctgtccg ctaagtcttt cgacggtcgt ggtaactaca
     8281 gcatgggtgt ccgtgagcag atcatcttcc cagaaatcga ctacgataaa gtcgaccgcg
     8341 ttcgtggttt ggatattacc attaccacta ctgcgaaatc tgacgaagaa ggccgcgctc
     8401 tgctggctgc ctttgacttc ccgttccgca agtaaggtag ggttactaaa tggctaagca
     8461 atcaatgaaa gcacgcgaag taaaacgcgt agctttagct gataaatact tcgcgaaacg
     8521 cgctgaactg aaagcgatca tctctgatgt gaacgcttcc gacgaagatc gttggaacgc
     8581 tgttctcaag ctgcagactc tgccgcgtga ttccagcccg tctcgtcagc gtaaccgctg
     8641 ccgtcaaaca ggtcgtccgc atggtttcct gcggaagttc gggttgagcc gtattaaggt
     8701 ccgtgaagcc gctatgcgcg gtgaaatccc gggtctgaaa aaggctagct ggtaattgtc
     8761 accaattgaa tcacgggagt aaagacagat gagcatgcaa gatccgatcg cggatatgct
     8821 gacccgtatc cgtaacggtc aggccgcgaa caaagctgcg gtcaccatgc cttcctccaa
     8881 gctgaaagtg gcaatcgcca acgtgctgaa ggaagaaggt tttattgaag attttaaagt
     8941 tgaaggcgac accaagcctg aactggaact tactctgaag tatttccagg gcaaagctgt
     9001 tgtagaaagc attcagcgtg tcagccgccc aggtctgcgc atctataaac gtaaagatga
     9061 gctgccgaaa gttatggcgg gtctgggtat cgcagttgtt tctacctcta aaggtgttat
     9121 gactgatcgt gcagcgcgcc aggctggtct tggtggcgaa attatctgct acgtagccta
     9181 atcggaggaa aaaatgtctc gtgttgctaa agcaccggtc gttgttcctg ccggcgttga
     9241 cgtaaaaatc aacggtcagg ttattacgat caaaggtaaa aacggcgagc tgactcgtac
     9301 tctcaacgat gctgttgaag ttaaacatgc agataatacc ctgaccttcg gtccgcgtga
     9361 tggttacgca gacggttggg cacaggctgg taccgcgcgt gccctgctga actcaatggt
     9421 tatcggtgtt accgaaggct tcactaagaa gctgcagctg gttggtgtag gttaccgtgc
     9481 agcggttaaa ggcaatgtga ttaacctgtc tctgggtttc tctcatcctg ttgaccatca
     9541 gctgcctgcg ggtatcactg ctgaatgtcc gactcagact gaaatcgtgc tgaaaggcgc
     9601 tgataagcag gtgatcggcc aggttgcagc ggatctgcgc gcctaccgtc gtcctgagcc
     9661 ttacaaaggc aagggggttc gttacgccga cgaagtcgtg cgtaccaaag aggctaagaa
     9721 gaagtaaggt aacactatgg ataagaaatc tgctcgtatc cgtcgtgcga cccgcgcacg
     9781 ccgcaagctc caggagctgg gcgcaactcg cctggtggta catcgtaccc cgcgtcacat
     9841 ttacgcacag gtaattgcac cgaacggttc tgaagttctg gtagctgctt ctactgtaga
     9901 aaaagctatc gctgaacaac tgaagtacac cggtaacaaa gacgcggctg cagctgtggg
     9961 taaagctgtc gctgaacgcg ctctggaaaa aggcatcaaa gatgtatcct ttgaccgttc
    10021 cgggttccaa tatcatggtc gtgtccaggc actggcagat gctgcccgtg aagctggcct
    10081 tcagttctaa ggtagaggtg taagatggct cacatcgaaa aacaagctgg cgaactgcag
    10141 gaaaagctga tcgcggtaaa ccgcgtatct aaaaccgtta aaggtggtcg tattttctcc
    10201 ttcacagctc tgactgtagt tggcgatggt aacggtcgcg ttggttttgg ttacggtaaa
    10261 gcgcgtgaag ttccagcagc gatccagaaa gcgatggaaa aagcccgtcg caatatgatt
    10321 aacgtcgcgc tgaataacgg cactctacaa caccctgtta aaggtgttca cacgggttct
    10381 cgcgtattca tgcagccggc ttccgaaggt accggtatca tcgccggtgg tgcaatgcgc
    10441 gccgttctgg aagtcgctgg ggttcataac gttctggcta aagcatatgg ttccaccaac
    10501 ccgatcaacg tggttcgtgc aactattgat ggcctggaaa atatgaattc tccagaaatg
    10561 gtcgctgcca agcgtggtaa atccgttgaa gaaattctgg ggaaataaac catggcaaag
    10621 actattaaaa ttactcaaac ccgcagtgca atcggtcgtc tgccgaaaca caaggcaacg
    10681 ctgcttggct tgggtctgcg tcgtattggt cacaccgtag agcgcgagga tactcctgct
    10741 attcgcggta tgatcaacgc ggtttccttc atggttaaag ttgaggagta agagatgcgt
    10801 ttaaatactc tgtctccggc cgaaggctcc aaaaaggcgg gtaaacgcct gggtcgtggt
    10861 atcggttctg gcctcggtaa aaccggtggt cgtggtcaca aaggtcagaa gtctcgttct
    10921 ggcggtggcg tacgtcgcgg tttcgagggt ggtcagatgc ctctgtaccg tcgtctgccg
    10981 aaattcggct tcacttctcg taaagcagcg attacagccg aagttcgtct atctgacctg
    11041 gctaaagtag aaggcggtgt tgtagacctg aacacgctga aagcggctaa cattatcggt
    11101 atccagatcg agttcgcgaa agtgatcctg gctggcgaag taacgactcc ggtaactgtt
    11161 cgtggcctgc gtgttactaa aggcgctcgt gctgctatcg aagctgctgg cggtaaaatc
    11221 gaggaataag tagcagatgg ctaaacaacc gggattagat tttcaaagtg ccaaaggtgg
    11281 cttaggcgag ctgaaacgca gactgctgtt tgttatcggt gcgctgattg tgttccgtat
    11341 tggctctttt attccgatcc ctggtattga tgccgctgta cttgccaaac tgcttgagca
    11401 acagcgaggc accatcattg agatgtttaa catgttctct ggtggtgctc tcagccgtgc
    11461 ttctatcttt gctctgggga tcatgccgta tatttcggcg tcgatcatta tccagctgct
    11521 gacggtggtt cacccaacgt tggcagaaat taagaaagaa ggggagtctg gtcgtcgtaa
    11581 gatcagccag tacacccgtt acggtactct ggtgctggca atattccagt cgatcggtat
    11641 tgctaccggt ctgccgaata tgcctggtat gcaaggcctg gtgattaacc cgggctttgc
    11701 attctacttc accgctgttg taagtctggt cacaggaacg atgttcctga tgtggttggg
    11761 cgaacagatc actgaacgag gtatcggcaa cggtatttca atcattatct tcgccggtat
    11821 tgtcgcggga ctcccgccag ccattgccca tactatcgag caagcgcgtc aaggcgacct
    11881 gcacttcctc gtgttgctgt tggttgcagt attagtattt gcagtgacgt tctttgttgt
    11941 gtttgttgag cgtggtcaac gccgcattgt ggtaaactac gcgaaacgtc agcaaggtcg
    12001 tcgtgtctat gctgcacaga gcacacattt accgctgaaa gtgaatatgg cgggggtaat
    12061 cccggcaatc ttcgcttcca gtattattct gttcccggcg accatcgcgt catggttcgg
    12121 gggcggtact ggttggaact ggctgacaac aatttcgctg tatttgcagc ctgggcaacc
    12181 gctttatgtg ttactctatg cgtctgcaat catcttcttc tgtttcttct acacggcgtt
    12241 ggttttcaac ccgcgtgaaa cagcagataa cctgaagaag tccggtgcat ttgtaccagg
    12301 aattcgtccg ggagagcaaa cggcgaagta tatcgataaa gtaatgaccc gcctgacctt
    12361 ggttggtgcg ctgtatatta cctttatctg cctgatcccg gagttcatgc gtgatgcaat
    12421 gaaagtaccg ttctacttcg gtgggacctc actgcttatc gttgttgtcg tgattatgga
    12481 ctttatggct caagtgcaaa ctctgatgat gtccagtcag tatgagtctg cattgaagaa
    12541 ggcgaacctg aaaggctacg gccgataatt ggtcgcccga gaagttacgg agagtaaaaa
    12601 tgaaagttcg tgcttccgtc aagaaattat gccgtaactg caaaatcgtt aagcgtgatg
    12661 gtgtcatccg tgtgatttgc agtgccgagc cgaagcataa acagcgccaa ggctgatttt
    12721 ttcgcatatt tttcttgcaa agttgggttg agctggctag attagccagc caatcttttg
    12781 tatgtctgta cgtttccatt tgagtatcct gaaaacgggc ttttcagcat ggaacgtaca
    12841 tattaaatag taggagtgca tagtggcccg tatagcaggc attaacattc ctgatcataa
    12901 gcatgccgta atcgcattaa cttcgattta tggcgtcggc aagacccgtt ctaaagccat
    12961 cctggctgca gcgggtatcg ctgaagatgt taagatcagt gagctgtctg aaggacaaat
    13021 cgacacgctg cgtgacgaag ttgccaaatt tgtcgttgaa ggtgatctgc gccgtgaaat
    13081 cagcatgagc atcaagcgcc tgatggatct tggttgctat cgcggtttgc gtcatcgtcg
    13141 tggtctcccg gttcgcggtc agcgtaccaa gaccaacgca cgtacccgta agggtccgcg
    13201 caaaccgatc aagaaataat cggggtgatt gaataatggc aaaggcacca attcgtgcac
    13261 gtaaacgtgt aagaaaacaa gtctctgacg gcgtggctca tatccatgct tctttcaaca
    13321 acaccatcgt gactatcact gatcgtcagg gtaacgcgtt gggttgggca acagccggtg
    13381 gttccggttt ccgtggttct cgcaaatcca ctccgtttgc agctcaggtt gcagcagagc
    13441 gttgcgctga cgccgtgaaa gaatacggca tcaagaatct ggaagttatg gttaaaggtc
    13501 cgggtccagg ccgcgaatct actattcgtg ctctgaacgc cgcaggtttc cgcatcacta
    13561 acattactga tgtgactccg atccctcata acggttgtcg tccgccgaaa aaacgtcgcg
    13621 tataacgcct cgttttccag gtttgttgga gaaagaaaat ggcaagatat ttgggtccta
    13681 agctcaagct gagccgtcgt gagggcaccg acttattcct taagtctggc gttcgcgcga
    13741 tcgataccaa gtgtaaaatt gaacaagctc ctggccagca cggtgcgcgt aaaccgcgtc
    13801 tgtctgacta tggtgtgcag ttgcgtgaaa agcaaaaagt tcgccgtatc tatggtgtgc
    13861 tggagcgtca gttccgtaac tactacaaag aagcagcacg tctgaaaggc aacaccggtg
    13921 aaaacctgtt ggctctgctg gaaggtcgtc tggacaacgt tgtataccgt atgggcttcg
    13981 gtgccactcg tgcagaagca cgtcagctgg ttagccataa agcaattatg gtaaacggtc
    14041 gtgttgttaa catcgcttct tatcaggtta gtccgaatga cgttgtaagc attcgtgaga
    14101 aagcgaagaa gcagtctcgc gtgaaagccg ctctggagct ggctgagcag cgtgaaaagc
    14161 caacttggct ggaagttgat gctggcaaga tggaaggtac gtttaagcgt aagccggagc
    14221 gttctgatct gtctgcggac attaacgaac acctgatcgt cgagctttac tccaagtaaa
    14281 gcttagtacc aaagagagga cacaatgcag ggttctgtga cagagtttct aaaaccgcgc
    14341 ctggttgata tcgagcaagt gagttcgacg cacgccaagg tgacccttga gcctttagag
    14401 cgtggctttg gccatactct gggtaacgca ctgcgccgta ttctgctctc atcgatgccg
    14461 ggttgcgcgg tgaccgaggt tgagattgat ggtgtactac atgagtacag caccaaagaa
    14521 ggcgttcagg aagatatcct ggaaatcctg ctcaacctga aagggctggc ggtgagagtt
    14581 cagggcaaag atgaagttat tcttaccttg aataaatctg gcattggccc tgtgactgca
    14641 gccgatatca cccacgacgg tgatgttgaa atcgtcaagc cgcagcacgt gatctgccac
    14701 ctgaccgatg agaacgcgtc tattagcatg cgtatcaaag ttcagcgcgg tcgtggttat
    14761 gtgccggctt ctacccgaat tcattcggaa gaagatgagc gcccaatcgg ccgtctgctg
    14821 gtcgacgcat gctacagccc tgtggagcgt attgcctaca atgttgaagc agcgcgtgta
    14881 gaacagcgta ccgacctgga caagctggtc atcgaaatgg aaaccaacgg cacaatcgat
    14941 cctgaagagg cgattcgtcg tgcggcaacc attctggctg aacaactgga agctttcgtt
    15001 gacttacgtg atgtacgtca gcctgaagtg aaagaagaga aaccagagtt cgatccgatc
    15061 ctgctgcgcc ctgttgacga tctggaattg actgtccgct ctgctaactg ccttaaagca
    15121 gaagctatcc actatatcgg tgatctggta cagcgtaccg aggttgagct ccttaaaacg
    15181 cctaaccttg gtaaaaaatc tcttactgag attaaagacg tgctggcttc ccgtggactg
    15241 tctctgggca tgcgcttgga aaactggcca ccggcaagca tcgctgacga gtaaccggat
    15301 cacaggttaa ggttttactg agaaggataa ggtcatgcgc catcgtaaga gtggtcgtca
    15361 actgaaccgc aacagcagcc atcgccaggc tatgttccgc aatatggcag gttcactggt
    15421 tcgtcatgaa atcatcaaga cgactctgcc taaagcgaaa gagctgcgcc gcgtagttga
    15481 gccgctgatt actcttgcca agactgatag cgttgctaat cgtcgtctgg cattcgcccg
    15541 tactcgtgat aacgagatcg tggcaaaact gtttaacgaa ctgggcccgc gtttcgcgag
    15601 ccgtgccggt ggttacactc gtattctgaa gtgtggcttc cgtgcaggcg acaacgcgcc
    15661 gatggcttac atcgagctgg ttgatcgttc agagaaagca gaagctgctg cagagtaatc
    15721 tgaagcaatg taaaaaaacc cgccccggcg ggttttttta tacccgtagt atccccactt
    15781 atctacaata gctgtactct ttt
//
LOCUS       DAJTRD010000087        15523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTRD010000087 DAJTRD010000000
VERSION     DAJTRD010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.087"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..73)
                     /locus_tag="OO315_003097"
     CDS             complement(<1..73)
                     /locus_tag="OO315_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5891914.1"
                     /translation="MTMKKLLIIILPVLLSGCSAFNQL"
     gene            complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO315_003098"
     CDS             complement(171..1280)
                     /gene="anmK"
                     /locus_tag="OO315_003098"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5891915.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1554..2021
                     /gene="slyB"
                     /locus_tag="OO315_003099"
     CDS             1554..2021
                     /gene="slyB"
                     /locus_tag="OO315_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5891916.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO315_003100"
     CDS             complement(2068..2502)
                     /gene="slyA"
                     /locus_tag="OO315_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5891917.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2703..2939
                     /locus_tag="OO315_003101"
     CDS             2703..2939
                     /locus_tag="OO315_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5891918.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2942..3799
                     /locus_tag="OO315_003102"
     CDS             2942..3799
                     /locus_tag="OO315_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5891919.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3799..5811
                     /locus_tag="OO315_003103"
                     /pseudo
     CDS             3799..5811
                     /locus_tag="OO315_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO315_003104"
     CDS             complement(5812..6333)
                     /gene="sodC"
                     /locus_tag="OO315_003104"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5891920.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6414..7310)
                     /locus_tag="OO315_003105"
     CDS             complement(6414..7310)
                     /locus_tag="OO315_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5891921.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7359..7598)
                     /locus_tag="OO315_003106"
     CDS             complement(7359..7598)
                     /locus_tag="OO315_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5891922.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7701..8300
                     /gene="nemR"
                     /locus_tag="OO315_003107"
     CDS             7701..8300
                     /gene="nemR"
                     /locus_tag="OO315_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5891923.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8337..9434
                     /gene="nemA"
                     /locus_tag="OO315_003108"
     CDS             8337..9434
                     /gene="nemA"
                     /locus_tag="OO315_003108"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5891924.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9515..9922
                     /gene="gloA"
                     /locus_tag="OO315_003109"
     CDS             9515..9922
                     /gene="gloA"
                     /locus_tag="OO315_003109"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5891925.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            10025..10672
                     /gene="rnt"
                     /locus_tag="OO315_003110"
     CDS             10025..10672
                     /gene="rnt"
                     /locus_tag="OO315_003110"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5891926.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10765..12879
                     /locus_tag="OO315_003111"
     CDS             10765..12879
                     /locus_tag="OO315_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5891927.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12889..14181
                     /locus_tag="OO315_003112"
     CDS             12889..14181
                     /locus_tag="OO315_003112"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891928.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            15131..15223
                     /locus_tag="OO315_003113"
     CDS             15131..15223
                     /locus_tag="OO315_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR5891929.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15267..>15523)
                     /locus_tag="OO315_003114"
     CDS             complement(15267..>15523)
                     /locus_tag="OO315_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5891930.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         3560 a         4109 c         4078 g         3776 t
ORIGIN      
        1 ccagttgatt aaaggcgcta caaccggaga gaaggaccgg caaaatgata attaacagtt
       61 ttttcatagt catatcccga agactttcct ggtccggagg gcaaataccc tccttaatct
      121 tccaagtgta acggcagaca cgataagaaa aaattcagtt aattctgata tcacgagttg
      181 gcggggaaaa tagcccccag taccgtctcc tggcttgcgc cagtgacgga aggcagattt
      241 cctggtaatc ccgccagcgt ccgccaggca agccaggcga aagccaatgc ttccatgtca
      301 tcgccactaa tgccaacggc atcggtggtg gtgacttctg tgcctagcag taataccgcc
      361 agacgcgcca tgagtagcgg attacgacta cctccaccac ataccatcaa tcgttcgcag
      421 ccaccgctca acaaaacttg ttcagaaatg gtcacggcgg tgagttctgc cagtgtggcc
      481 tgaacatcac ggggatcaac acccggaaaa tggcgtaaat ggcgctccag ccagccgtag
      541 ttaaagtatt cgcgtccggt gcttttcggt gcaggttgcg agaaatacgg gtcgctgagc
      601 atattttgca gcagtgggag aataacttta cccgcccgtg cccactcggc atctttatcg
      661 taaggtttac ctgcctgacg ccagatccag gcatccatca gcatgttacc aggaccggta
      721 tcgtagccgc caaccagctg cccaggaatg agcagtgaca gattggcgat accgccaata
      781 ttgagcacca ttcgtcgctc ggttgggtga gccagcaagg catgatggaa cgcaggtacc
      841 agcggtgcgc cttgtcctcc caaggcaata tcgcgacggc gaaaatcacc gacaaccgta
      901 attccggtgc gtgccacaat ttgattgtta tcgccaatct gtaaagtgtg tggtgccacg
      961 cccgtcggtt catgccagac ggtttgaccg tgacagccga tcgcaactat atctctcgcc
     1021 tgcaggtttt gttccttaag caaggcattg accgcatcag caaaaagtcg cccgagttga
     1081 gtatcaagct gtccaaactg cgaaagtgta agctgctggc cctggcaaat atccagtaca
     1141 gcctgtttca gagacaccgg gatcggccag ctcaagcttg ccagctgtgc gacccggtgt
     1201 tcatcaattg tcgccaacac aacatcaaca ccatcaaggc tggtgcctga cataacgcca
     1261 ataaagcggc ccgatttcat agttcatcct ttttcaatct gacgtttgcg caccactcaa
     1321 acataaactt ttcgtgaata ccatgcggaa tgaccgattt ttaccgttgg tagtaaaaca
     1381 ttatcttcaa atcaataatc atcatgaatg ttttgtttat aattggttga tcctactttc
     1441 attatgattt gctcatattt ggtagaacat gtaaccatgg attcacatat gccatatact
     1501 ttgaccatga gggatgcttg cgtggcgttt catggtgaac aggagatttt tcaatgatta
     1561 aacgcgtatt ggttgtttca atggtaggtc tgtctcttgt cggttgtgtt aataacgaca
     1621 ccctgtcagg ggatgtttat accgcttctg aagcgaaaca agtacagaat gtcagctatg
     1681 gcaccatcgt taacgtacgt ccggtacaga ttcagggcgg tgatgattcc aacgttatcg
     1741 gtgcaattgg cggtgctgtt cttggtggtt tcctggggaa tactgttggt ggcggaaccg
     1801 ggcgttctct ggctactgca gcaggcgctg ttgcaggtgg cgtagctggt cagggcgtac
     1861 agagtgcaat gaacaaaacg cagggtgtcg agctggaaat tcgtaaagac gatggtaata
     1921 ccatcatggt ggtacagaaa caaggcaaca ctcgtttctc tccgggccaa cgtgtcgtac
     1981 tggccagcaa tggcagtcag gtgaccgttt ctccgcgcta aataagtttg cgtgtggtca
     2041 ggttactgac cacacgcccc cttcatttca ccctttggcc tgtaactcaa tgatattatg
     2101 ctcaagtttt gcgatgagcg taatcagttg ctccagttcc tctgcggaga tgccatgtaa
     2161 tatttccgcg cgggttttgt taataacagc ttccatttcg ctgatcagtg gctctgcctt
     2221 ttccgtcagt ttaatacgtt tagcccgacg atcgctggca caagtttgac gcgaaattaa
     2281 ccctttttct tccagttggt ccagagtacg gaccagtgat ggctgctcga tgccaatcgc
     2341 ttttgccagt tgaatttgcg actggtctgg aggtaactga tggatattgt gtaacgtaac
     2401 ccaatgggtt tgtgttaact ccagcggttt caggcgatgg tctatcagag cacgccatat
     2461 gcgcaccaac cgtgccagat cagaacctag tggcgattcc aatttcatct ccttataatt
     2521 agcttgctaa gatattatgc ggcttttaga atagtgtgca gcaattgtat tgctaaaaca
     2581 aatgtattgc tgcatttggt taccgtcaga catatttttc agaaattgcg cgtaaatttt
     2641 tcgcacttaa agaatattta ttaatctaac gcaatatatt cggtcgtaaa ggaatctact
     2701 ttgtgaagtt tatgctcaat gcaacaggat tgcccttaca agatctggtg ttcggtgcgt
     2761 ccgtctattt tcctccgttt ttcaaagcat tcgcgtttgg attcgtcatc tggcttgtcg
     2821 tacaccgcct gcttcgtggc tggatctacg ccggcgatat ctggcatccc ttgttaatgg
     2881 atttatcgct gtttgcgatt tgcgtttgcc ttgctctggc aatactgatt gcgtggtaac
     2941 tatgtcaatt aaaacaatta agtatttctc aacaatcatt gtagcgatag ttgcggttct
     3001 tgccggatgg tggctgtgga attattacat gcaatctccg tggacacgcg acgggaaaat
     3061 acgcgcagaa caggtttcta tcaccccaca ggtgtcggga cgtattgttg agctgaatat
     3121 aaaagacaac caactggtta acgcggggga acttctgctc acgatcgata aaacgccctt
     3181 tcagatcgcc gagctgaacg cacaggctca gctggcgaaa gcgcaatctg acctcgcaaa
     3241 agccaataac gaagcgaatc gtcgtcgtca tctctcacaa aattttattt ttgccgaaga
     3301 actcgatacc gctaacctca atgttaaagc gatgcaggcc agtgttaatg ccgcacaggc
     3361 gacgctgaaa cagacacaat ggcaactggc gcaaacggaa attcgcgccc cggtgagtgg
     3421 atgggtgact aacctcacta cccgcatcgg cgattacgct gacaccggga aaccgctgtt
     3481 tgcccttgtc gacagccact cgttttacgt cattggttat tttgaagaaa ccaagttgcg
     3541 ccatatccgc gaaggtgcac cagcacaaat taccctctat agtgataaca aaacgttaca
     3601 gggtcacgtt tccagtatcg gtcgggcgat ttatgatcag agcgttgaaa gtgactcaag
     3661 ccttattccg gatgtgaaac ctaacgtccc ctgggttcgt ctcgcccaac tcgttcccgt
     3721 ccgctttgcg ctggataaag ttccaggcga tgtcacgctg gtgtcgggca ccacctgtag
     3781 cattgccgta ggtcaataat gaacgcatcg tcatggtcct tgcgcaattt gccctggttc
     3841 agggccacgc tggcgcaatg gcgttatgcg ttacgcaata ccattgccat gtgtctggcg
     3901 ctgacggttg cctattattt gaatctggat gaaccctatt gggcgatgac ctcggctgca
     3961 gtggttagct ttcccaccgt tggcggtgtc atcagcaaaa gcctcggacg catggctggc
     4021 agtttactgg gagccattgc ggcactgctt cttgccggac atacgctcaa tgagccgtgg
     4081 ttttttctat tgagcatgtc ggcgtggctt ggcttttgta cctaggcctg tgcgcacttc
     4141 acgaataacg tcgcgtatgc atttcaactg gcgggctaca cggctgccat catcgccttt
     4201 ccgatggtta atattactga ggccagccag ctgtgggata tcgctcaggc gcgcgtttgc
     4261 gaggtgatag tcggtatttt gtgcggcggc atgatgatga tgatcctgcc tagcagttcc
     4321 gatgctactg cccttttaac tgcattgaaa aacatgcacg cccgattact ggaacatgcc
     4381 agtttactct ggcagcctga aacaaacgat gccattcgtg cagcacatga gggggtgatt
     4441 gggcagatac tgaccatgaa tttgctgcgt atccaggctt tctggagcca ctatcgtttt
     4501 cgccagcaaa acgcgcgcct taatgcgcta ctccaccagc aattacgtat gaccagtgtc
     4561 atctccagcc tgcgacgtat gttgctcaac tggccctcac cgccaggtgc cacgcgagaa
     4621 attctcgaac aattgctgac ggcgctcgcc agttcgcaaa cagatgctta caccgtcgca
     4681 cgtattatcg ccccgctacg cccgaccaac gtcgccgact accggcacgt cgccttctgg
     4741 cagcgactac gttatttttg ccgcctttat ctgcaaagta gtcaggaatt acatcgtctg
     4801 caaagcggtg tagatgatca tgccagactc gcacggacat ccgctctggc tcgtcatacc
     4861 gacaacgccg aagctatgtg gagcgggctg cgtacatttt gtacgttgat gatgattggc
     4921 gcatggagta ttgcatcgca atgggatgcc ggtgccaatg cattaacgct ggcagcaatt
     4981 agctgcgtac tctactccgc cgtcgcagca ccgtttaagt cgttgtcact gctgatgcgc
     5041 acgctggtgt tactttcgct attcagcttt gtggtcaaat ttggcctgat ggtccagatt
     5101 agcgatctgt ggcaattttt actgtttctc tttccactgc tggcgacaat gcagcttctt
     5161 aaattgcaga tgcccaaatt tgccgcactg tgggggcaac tgattgtttt tatgggttcg
     5221 tttatcgctg tcactaatcc cccggtgtat gattttgctg attttcttaa ctataatctg
     5281 gcaaaaatcg ttggcgttgc gctggcttgg ctagcgttcg ccattctgcg tccaggatcg
     5341 gatgctcgta aaagccgccg ccatattcgc gcgctgcgcc gggattttgt cgatcagcta
     5401 agccgccatc caacactgag tgaaagcgaa tttgaatcgc tcacttatca tcacgtcagt
     5461 cagttgagta acagccagga tgcgctggct cgccgttggt tattacgctg gggtgtagtg
     5521 ctgctgaact gttctcatgt tgtctggcaa ttgcgcgact gggaatcgcg ttccgatccg
     5581 ttatcgcgag tacgggataa ctgtatttca ctgttgcggg gagtgatgag tgagcgtggc
     5641 gttcagcaaa aatcactggc ggccacactt gaagaattac agcggatttg cgacagcctt
     5701 gcccgtcatc atcaacctgc cgcccgtgag ctggcggcaa ttgtctggcg gctgtactgc
     5761 tcgctttcgc aacttgagca agcaccaccg caaggtacgc tggcctcgta attacttaat
     5821 tacaccacag gcatagcgtt caccgccacc gcccagcggt ttaggttgat cggacatatt
     5881 atcgccgcca acgtggacca tcagcgcctt gtctttgatt tcatccagtg atttcagacg
     5941 aggcgcgatg acggcatcgg tagctttgcc gtcattattg acgaccagtg caggcagatc
     6001 gcctaaatgc ccggtacctt ctggcccttc atgtttaccg gtattttgtg gatcaagatg
     6061 cccgcctgcg gattccgcgg cgctggcttt gccatctttg gtggctggct ggcagcttcc
     6121 tttggcatga atatggaagc catgttcacc ggggggtaat gctttcagat cgggtgaaaa
     6181 ctccagacct ttatcggttt cagtaatggt gacgctacca attgactgcc caaccccttg
     6241 cgacgtgacg aggttcatct cgactttttc actggcagct tgtgcgccgg tcgcaacaac
     6301 cagcgccaga atggccagac taaaacgttt cataggacct ccgttcattg tgcattcctg
     6361 aataagtgta aaccagtgac acatttttga acggcggcta ttcctaaaag tgcttacggt
     6421 acgtcgtacc ccagtgcagc tttacggata cgaaaccatt gttgacgggt cattttcagt
     6481 gtttctgctt cgacagctgc ccgtacgcgc tcaattttac ctgagccgat aattggcagc
     6541 ggctgcgacg gtaaacgtaa tacccaggcg taaaccacct gttcaatcga gcccgcgttt
     6601 aactcctccg ccaccacagc cagttcatca cgcagcggct ggaaataatc atcattaaac
     6661 agacgaccac caccaaggca ggaccacgcc atcggacgaa cacgcagttg ttgtagttgg
     6721 tcgagcgtgc catccagcag taacggctga tgcaccgggg atatttccac ctgattagtg
     6781 gcaagggtaa acggcagacg tgattgcaac agggcaaatt gcgcaggcgt aaagttcgat
     6841 acgccaaaat gacgcacttt gccgctctga tgcagatgtt tgaacgcgtc cgccacttca
     6901 tcggcatcca ttaacgggtc tggtcggtgg attaacagca gatccagatg atcggtcgcg
     6961 agattaatta gcgactgttc ggcgctctta atgatgtgat tgcggtcagt gatgtaatga
     7021 ccaatgacgt tttcttcacg cgcggtcgtc gcgataccgc atttactgac gatttccatc
     7081 cgttcacgca ggtgaggtgc cagtttcagt gcctcgccaa acgccgcttc gcactgatag
     7141 ccaccataaa tatcagcatg gtccacggtg gtcacgccga gatccagatg ctcttcaata
     7201 aaactgacca gctggcgggc ggacatattc cagtccatca atcgccagta gcccatcaca
     7261 aaacgggaaa actccgggcc ttccggcgca atagtaatac gctgaaccat aatcgcttcc
     7321 tcttatcaga tataagagga gtatacgcaa gattaggttc aaaagagtga tggttgctcc
     7381 ggttcgtctg atgacgctgg cttatttgcg cgtaatttgc gcattaatcg ctgccgacaa
     7441 aggcgcagca cctcttgttt ttcgccatcg ctcattttat tccagttaaa acgctcatcc
     7501 cgactacgaa aacagccgcg acaaaacccg cgttcgtcag actggcaaat tccccggcac
     7561 gggctctgga cgggaaagaa ctctaattgc tccgccacct cgccctcctc agataagatt
     7621 attaccatta ttgaagctgt taatgtccaa agtagcaact ttgcttgcac tagaccaact
     7681 ggtctactac actccaacgc atgaacaaac acaccgaaca tgatactcgc gaacatctct
     7741 tggcgacggg cgagcaactt tgcctgcaac gtggattcac cgggatgggg ctaagcgaat
     7801 tactaaaaac cgctgaagtg ccgaaagggt ccttctatca ctactttcgc tctaaagaag
     7861 cgtttggcgt tgccatgctt gagcgtcatt acgccgcata tcaccagcga ctggctgagt
     7921 tgctgcaatc cggcgaaggt aactaccgcg accgcatact ggcttattac cagcaaacac
     7981 tgaaccagtt ttgccaacat ggaaccatca gtggttgcct gacagtaaaa ctctctgccg
     8041 aagtgtgcga tctgtcagaa gatatgcgca gcgcgatgga taaaggtgct cgcggcgtga
     8101 tcgccctgct ctcgcaggct ctggaaaatg gccgtgagaa ccattgttta accttttgtg
     8161 gcgaaccgct acaacaggca caagtgcttt acgcactatg gcttggcgcg aatctgcagg
     8221 ccaaaatttc gcgcagtttc gagccactgg aaaacgcgct ggcccatgta aaaaacatta
     8281 ttgcgacgcc tgccgtttag caggcatttt ttatcaccag acgaccggga gcctttatgt
     8341 catctgaaaa actgtattcc ccactgaaag tgggcgcgat cacggcggca aaccgtattt
     8401 ttatggcacc gctgacgcgt ctgcgcagta ttgaaccggg tgacattcct accccgttga
     8461 tggcggaata ctatcgccaa cgtgccagtg ccggtttgat tattagtgaa gccacgcaaa
     8521 tttctgccca ggcaaaagga tatgcaggtg cgcctggcat ccatagtccg gagcaaattg
     8581 ccgcatggaa aaaaatcacc gctggcgttc atgctgaaaa tggtcatatg gccttgcagc
     8641 tgtggcacac cggacgcatt tctcacgcca gcctgcaacc tggcggtcag gcaccggtag
     8701 cgccttcagc acttagcgcg ggaacacgta cttctctgcg cgatgaaaat ggtcaggcga
     8761 tccgcgttga aacatccatg ccgcgtgcgc ttgaactgga agagattccg ggtatcgtca
     8821 atgatttccg tcaggccatt gctaacgcgc gtgaagccgc ttttgatctg gtagagctcc
     8881 actctgctca cggttatttg ctgcatcagt tcctttcccc ttcttcaaac catcgtaccg
     8941 atcagtacgg cggcagcgtg gaaaatcgcg cacgtttggt actggaagtg gtcgatgccg
     9001 ggattgaaga atggggtgcc gatcgcattg gcattcgcgt ttcgccaatc ggtactttcc
     9061 agaacacaga taacggcccg aatgaagaag ccgatgcact gtatctgatt gaacaactgg
     9121 gtaaacgcgg cattgcttat ctgcatatgt cagaaccaga ttgggcgggg ggtgaaccgt
     9181 atactgatgc gttccgcgaa aaagtacgcg cccgtttcca cggtccgatt atcggcgcag
     9241 gtgcatacac agtagaaaaa gctgaaacgc tgatcggcaa agggttaatt gatgcggtgg
     9301 catttggtcg tgactggatt gcgaacccgg atctggtcgc ccgcttgcag ctcaaagctg
     9361 agcttaaccc acagcgtgcc gaaagtttct acggtggcgg cgcggaaggc tataccgatt
     9421 acccgacgtt gtaatccaac attgcgagcg gcgtaaagcc gccgctatac taaaacaaca
     9481 ttttgaatct gttagccatt ttgaggataa aaagatgcgt cttcttcata ccatgctgcg
     9541 cgttggcgat ttgcaacgct ccatcgattt ttataccaaa gtgctgggca tgaaactgct
     9601 gcgtaccagc gaaaacccgg aatacaaata ctcactggcg tttgttggct acggcccgga
     9661 aaccgaagaa gcggtgattg aactgaccta caactggggc gtggataaat acgaactcgg
     9721 cactgcttat ggtcacatcg cgcttagcgt agataacgcc gctgaagcgt gcgaaaaaat
     9781 ccgtcaaaac gggggtaacg tgacccgtga agcgggtccg gtaaaaggcg gtactacggt
     9841 tatcgcgttt gtggaagatc cggacggtta caaaattgag ttaatcgaag agaaagacgc
     9901 cggtcgcggt ctaggcaact aatctcctgc cgggcgtgaa ctcatcgcgc ccgcatcttt
     9961 actgcatcga caagtaatat ttgtcataat gcgcgctgca atttatccgt attaagagaa
    10021 tcagatgtcc gataacgctc aacttaccgg tctgtgcgac cgttttcgtg gtttttatcc
    10081 tgttgtgatc gatgttgaaa cagccggatt taacgccaaa accgatgcgc tgcttgagat
    10141 tgccgccatc accctgaaaa tggatgaaca aggctggctg atgccggaca ccacattaca
    10201 tttccacgtc gaaccatttg tcggcgcaaa tttgcaacca gaagccctcg ccttcaacgg
    10261 cattgacccg aacgatcccg atcgcggcgc ggtcagcgaa tacgaggcgc tgcacgaaat
    10321 ttttaaagtt gtacgtaaag gtattaaagc gagcggctgt aaccgcgcca ttatggtggc
    10381 gcacaacgcc aattttgatc acagctttat gatggccgcc gcagaacgcg cctcgctgaa
    10441 acgtaacccg ttccaccctt tcgccacttt tgacactgct gcactggccg ggctggcact
    10501 cggacaaacc gtattgtcaa aggcttgcca gaccgctggc atggacttcg acagcaccca
    10561 ggcgcactcc gcgctgtacg acaccgaacg cactgctgtg ctgttttgtg aaatcgtcaa
    10621 ccgctggaaa cgtctgggag gctggccgct acctgccgcc gaagaggtgt aatcgagtcg
    10681 atgcctgatg acatgcaatg attcaggcat ctatagtgag gctattccac gcatcaagca
    10741 tgatattcac ggggaatagc gttaatggca gataatccag acccttcatc gctcctgccg
    10801 gacgtgtttt caccggcgac ccgcgactgg tttcttcgcg cctttaaaca gccgaccgct
    10861 gtccagtcgc aaacctggca tgtggcggcg cgaggcgaac atgcgctggt gattgcaccg
    10921 actggctccg ggaaaacgct ggcagcattt ctctacgccc tcgatcggct cttccgcgaa
    10981 ggcggcgaag atacccgcga ggcgcataag cgtaaaacct cacgcatcct ctatatttca
    11041 ccgataaaag ccctgggcac cgacgttcag cgcaacttgc agatcccgtt gaagggtatt
    11101 gccgatgaac ggcggcggcg cggcgaaacg gtaattattg atgaagtcca cgcggtagcg
    11161 ggcagtaaac gtggtgcgca tctggcgtta agtctggagc ggctcgatgc gctgctccac
    11221 acctcagcac agcgaattgg cctttctgcc actgtgcgct cagccagcga tgtggcagca
    11281 tttcttggtg gcgatcgccc ggttacggta gtcaacccgc ccacaatgcg ccatccgcag
    11341 atacgaattg tcgtaccggt cgccaatatg gatgatgtct catcggtcgc cagcggcacc
    11401 ggcgaagaca gccatgccgg ccgggaaggc tccatctggc catatattga aacgggtatc
    11461 cttgatgaag tgttgcgcca tcgctcgacc attgtcttta ctaattcacg cgggctggcg
    11521 gaaaaactga cggcacgatt aaatgagctt tacgccgcac gcttacagcg ttccccgtct
    11581 atcgccgttg atgcggccca tttcgagtcg acctccggcg caacctctaa ccgtgtacaa
    11641 agtagcgacg tttttattgc ccgctcacac cacggctccg tctctaaaga acaacgagca
    11701 atcaccgaac aggcgctgaa atcgggtgaa ttacgctgcg tggtcgcaac ctccagtctt
    11761 gaactgggga ttgatatggg cgcggtggat ctggtgattc aggtggcaac gccgctttct
    11821 gttgccagtg ggttacaacg cattggtcgc gccggacatc aggttggcgg tgtatctaaa
    11881 gggctgtttt tcccccgtac ccggcgtgat ttagtcgatt ccgcagtcat tgtagagtgt
    11941 atgttcgcag gcaggctgga aaacctgaca ccaccgcata atcctctcga cgtccttgcg
    12001 cagcaaaccg ttgccgccgc ggcgatggat gcattacagg tagacgaatg gtactcccgc
    12061 gtacgccgta ccgcaccgtg gaaagatctg ccaagacgtg tttttgacgc cacgctggat
    12121 atgctttccg ggcgctatcc ctctggcgat ttttctgctt ttcgccccaa actggtctgg
    12181 aacagggaga ccgggatatt gaccgcccga cctggcgctc aattgttggc ggttaccagc
    12241 ggcggcacca ttccggatcg tggcatgtat agcgtgttat tacccgaagg tgaagaaaag
    12301 gccggttcgc ggcgggtggg tgaactggat gaggagatgg tatatgagtc gcgggtgaac
    12361 gacattatca ctctcggcgc tacctcatgg cggatccagc aaatcacccg cgatcaggtg
    12421 attgtgactc ctgctccggg tcgttctgcc cagctcccct tctggcgtgg tgaaggtaac
    12481 ggacgtccgg ctgaattagg cgagatgatc ggcgattttc ttcatttact ggcggatggc
    12541 gcgttctttt ccgggactat tcccccgtgg ctggcagaag aaaatacgaa cgccaatatt
    12601 caggggttga ttgacgagca acgcaacgcg acgggcatcg ttccggggag tcgccatctg
    12661 gtcctcgaac ggtgccgtga tgaaattggc gactggcgta ttattttgca ctctccctat
    12721 ggaagacggg tgcatgaacc ctgggcgctg gcgattgccg ggcgaataca tgccctatgg
    12781 ggcgctgacg cgtctgtggt cgccagtgat gacagcattg ttgcgcgcat tcctgacact
    12841 gatggtaaat tgcccgacgc cgcgattttt ttgttttaac cagaaaagtt gctgcaaatt
    12901 gtccgcgagg cggtaggcag ctcggcactt ttcgccgccc gttttcgcga atgcgccgcg
    12961 cgggcattat taatgccggg ccgcacgccg ggccatcgca ccccgctttg gcaacaacgg
    13021 ctgcgcgcca gtcagttgct ggaaatcgct cagggatatc cggattttcc ggtcattctc
    13081 gaaaccctac gcgaatgtct gcaagatgtt tatgatcttc ccgcactgga acgtttgatg
    13141 cgtcgcctga acggtggcga aattcaaata tccgatgtaa cgaccactac gccctcgcct
    13201 ttcgccacaa gtttattgtt cggctatgtc gcggaattta tgtaccagag cgacgccccg
    13261 ctggcagagc gccgggcatc cgtactgtcg ctggacagcg agttactgcg caatctactc
    13321 ggacaggtcg atccggggga attactcgac ccgcaggtca ttcgccaggt ggaagaagag
    13381 ttgcaacgac tggctcctgg tagaagagcg aaaggtgaag aaggattgtt cgacctgctg
    13441 cgcgaactgg ggccaatgac cgttgaagac ctggcgcaac ggcatacagg cagcagtgaa
    13501 gaggttgcgt cgtatctgga aaatcttctt gcagtaaaac gaatcttccc agcgatgatt
    13561 agcggacagg agcgtcttgc ctgtatggat gatgccgcca ggctgcgtga tgccctcggc
    13621 gtacggctac cagagtcatt gccagagatt tatttacata gagtcagtta cccgcttcgc
    13681 gacctctttc tgcgctatct ccgggctcat gctctggtca cggctgaaca actggctcat
    13741 gagtttagtc tcggtattgc cattgtcgaa gagcagcttc agcaactgcg tgaacagggt
    13801 ctggtgatga atctgcaaca agacatctgg gtgagcgatg aagtatttcg tcgtctgcgt
    13861 ttgcgctcgc tgcaagccgc cagagaagcg acgcgtcccg ttgcagccac gacctatgcg
    13921 cgattgctgc tggaacgtca gggcgtatta cccgccaccg atggtagccc ggcgctcttt
    13981 gcctcaacat ctccaggcgt ttatgagggc gtagatggag tgatgcgggt gatcgaacag
    14041 cttgccggag tcggtttacc cgcctcactc tgggaaagcc agatcctgcc tgcccgcgta
    14101 cgcgactatt cgccagaaat gctcgatgaa ttactggcaa ccggagcggt tatctggtcg
    14161 ggacaaaaaa aagctgggtg aagatgacgg cctggtggca ctgcatctac agaaatatgc
    14221 cgcagaatcg ttcactcccg ccgaagcgga tcaggcgaat cgttcggcgc tgcagcaagc
    14281 gataatcgct gttctggctg acggaggagc ctggtttgca caacaaatca gccaacggat
    14341 acgcgataaa atcgtcgaat cggttgattc ctcggcactg caagaggcgt tatgggcgct
    14401 ggtctggcaa ggcgtcatta ccagcgatat ttgggcaccg ttacgcgccc tcacccgcag
    14461 cagttccaac gcacgcacct caactcgccg cagtcaccgg gctcgtcgtg ggcgtcctgt
    14521 ctatgcacaa cccgtctcgc cgcgggtatc ttacaacaca ccaaatctgg ctggacgctg
    14581 gtcgttattg caggtggagc cactaaacga taccgaaagg atgctggcgc tggcggaaaa
    14641 tatgctcgac cgctacggca tcatcagtcg tcaggcggtg atagccgaaa atatccctgg
    14701 cgggtttcca tcgatgcaaa cactttgtcg aagtatggaa gactccgggc gaattatgcg
    14761 aggtcgtttt gtagaaggtc tgggtggcgc gcaattcgct gaacgtctga ctattgaccg
    14821 attgcgcgat ctggcgacac aagccacgca aacacgccac tatacaccag tggcgctctc
    14881 cgccaacgat ccggctaatg tgtggggaaa tcttctgccc tgggctgcac atccggcaac
    14941 gctggtacca acgcgtcggg cgggtgcgct ggtggtcgtt tctggcggca aattgttact
    15001 ctatctggcg caaggcggca aaaaaatgct ggtctggcag gaaaaggagg aattactcgc
    15061 cccagagatt ttccaggcgc tgaccaccgc actgcgtcgc gaaccaaggc tgcgctttac
    15121 gctaacagaa gtgaatgatc ttccggtccg gcaaacgccg atgtttacgc tgctgcgtga
    15181 ggcgggattt tcaagttcgc cacaagggct ggattgggga tagttgacat cctccacgcc
    15241 ctgaaagacg tggttccctg ctacgttcaa gctgtcgcct gaatcatttc ggtgggttcc
    15301 tgcttcaacg agcggcctga ctgcaccatc tctccacagg caagaacggc gtgccccgcc
    15361 gctaaaatgt tacgagcgcc atttacatcg gcgttcgctg tatatccaca tacctggcat
    15421 ctgaatttac tttgtgacag gcggttttct ttcgctgtat gaccacagca cgcgcaacgc
    15481 tggcttgtgt acgctggcgg aacagcaagc acctgaccgc cac
//
LOCUS       DAJTRD010000088        15438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTRD010000088 DAJTRD010000000
VERSION     DAJTRD010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.088"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO315_003115"
     CDS             complement(172..1197)
                     /gene="tdh"
                     /locus_tag="OO315_003115"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5891931.1"
                     /translation="MKALSKLKAEEGIWMTDVPVSELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO315_003116"
     CDS             complement(1207..2403)
                     /gene="kbl"
                     /locus_tag="OO315_003116"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5891932.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO315_003117"
     CDS             complement(2678..3535)
                     /gene="yibB"
                     /locus_tag="OO315_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5891933.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3839..4771
                     /gene="rfaD"
                     /locus_tag="OO315_003118"
     CDS             3839..4771
                     /gene="rfaD"
                     /locus_tag="OO315_003118"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5891934.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4781..5827
                     /gene="rfaF"
                     /locus_tag="OO315_003119"
     CDS             4781..5827
                     /gene="rfaF"
                     /locus_tag="OO315_003119"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5891935.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5831..6823
                     /gene="rfaC"
                     /locus_tag="OO315_003120"
     CDS             5831..6823
                     /gene="rfaC"
                     /locus_tag="OO315_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5891936.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6820..8028
                     /gene="rfaL"
                     /locus_tag="OO315_003121"
     CDS             6820..8028
                     /gene="rfaL"
                     /locus_tag="OO315_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5891937.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8064..9206)
                     /locus_tag="OO315_003122"
     CDS             complement(8064..9206)
                     /locus_tag="OO315_003122"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5891938.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9215..10228)
                     /locus_tag="OO315_003123"
     CDS             complement(9215..10228)
                     /locus_tag="OO315_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5891939.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO315_003124"
     CDS             complement(10253..10960)
                     /gene="rfaY"
                     /locus_tag="OO315_003124"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5891940.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO315_003125"
                     /gene_synonym="rfaI"
     CDS             complement(10986..11993)
                     /gene="waaO"
                     /locus_tag="OO315_003125"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5891941.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHVFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO315_003126"
     CDS             complement(12036..12833)
                     /gene="rfaP"
                     /locus_tag="OO315_003126"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5891942.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12826..13950)
                     /locus_tag="OO315_003127"
     CDS             complement(12826..13950)
                     /locus_tag="OO315_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5891943.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO315_003128"
     CDS             complement(13947..15005)
                     /gene="rfaQ"
                     /locus_tag="OO315_003128"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5891944.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            complement(15363..>15438)
                     /locus_tag="OO315_003129"
     CDS             complement(15363..>15438)
                     /locus_tag="OO315_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5891945.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         4407 a         3466 c         3164 g         4401 t
ORIGIN      
        1 tgtgagatat tatttttaca ggtgagagag tcacttcagg gtagagagat aatgagaaca
       61 gctatataaa agatcagcgc aattacgaac tctttattac actgacagga ttttacataa
      121 ttaacagatt atcctcagat aaaagggctg gaataccagc ccttgttcgt gttaatccca
      181 gctcagaata actttcccgg actggcccga acgcatagcg tcaaagccct tctggaaatc
      241 atcgatagag aaacgatggg taatgatcgg cgagagatcg aggccagact gaatcagcgc
      301 cgccatcttg taccaggttt caaacatctc acgaccgtaa atgcctttaa tgaacaagcc
      361 tttaaagatc actttggtcc agtcgataga catatcagac ggtggaatac ccagcatcgc
      421 aatacgaccg ccgtggttca tggtatcgag catggtacga aacgctggcg gcgcgccgga
      481 catttccaga ccgacatcaa agccttcggt catgcccagt tcagccatca catcattaag
      541 gttttctttc gcgacgttaa ccgcacgggt gatacccatt ttgcgcgcca gctcaaggcg
      601 gtattcgtta acatcggtga tcaccacatt gcgtgcacca acgtgtttcg ccaccgccgc
      661 agccataata cctatcggac ctgcaccaga aaccagcaca tcttcgccca ccagatcgaa
      721 cgacagcgcg gtatgcacgg cgttaccgaa gggatcaaaa atggaagcca ggtcgtcgga
      781 gatattgtcg gggattttga aagcgttgaa cgccggaatc accagatatt cggcgaagca
      841 gcccggacgg ttaacaccga caccaatggt gttgcggcac agatgggtac gaccgccacg
      901 acagttgcga cagtgaccgc aggtgatatg gccttcgcca gaaacgcgat cgccgatttt
      961 gaagcctttc acttcctgac caatacctac cacttcaccg acatattcat ggcccacgac
     1021 catcggcacc gggatggttt tttgcgacca ctcatcccag ttatagatgt gaacgtcagt
     1081 cccgcagatg gctgttttac ggattttaat cagcagatcg ttatgcccga gttccgatac
     1141 agggacgtcg gtcatccaga tgccctcttc cgctttcagt ttggataacg ctttcatctc
     1201 acatcctcag gcgataacgc ccagttgttt accaatacgc gtgaatgctt ctactgcacg
     1261 cgtaatttgc tcaggggtat gcgccgcaga catctgggta cgaatacgcg cctgaccttt
     1321 cggaacgacc ggatagaaga aaccggtgac gtaaatgccc tctttttgca gctctcgggc
     1381 aaatttctgc gccactaccg catcaccaag catgaccgga ataatggcgt gatcggctcc
     1441 cgccagggta aagcccgccg ccgacatttg ctcacggaac tgacgcgcgt tcgcccacag
     1501 acggtcacgc aactcgctac ccgcttcaac catctccagc actttgatgg aagcggcgac
     1561 aattgctggt gccagcgagt tggagaacag gtacggacga gaacgctggc gcagccactc
     1621 aaccacttct ttgcgcgccg cggtataacc accagaagcc ccgcccagcg ctttaccgag
     1681 cgtgccggtg atgatgtcga cacgccccat cacatcgcag tattcatggg aaccacgacc
     1741 attttcaccg acaaaaccga ccgcgtggga gtcgtctacc atcaccaggg catcgtattt
     1801 atctgccaga tcgcaaacgc ccttcagatt ggcaatcacg ccgtccatcg agaacacacc
     1861 atcggtggca atcagcacat gacgcgcacc ggcttcacgc gcttctttca gacgagcttc
     1921 aagctcctgc atatcgttgt tggcatagcg atagcgttta gctttacaca gacgcacacc
     1981 atcaataata gacgcgtggt tcagtgcgtc ggagataatg gcgtcttccg cgcccagcag
     2041 cgtttcaaac aggccaccgt tagcatcaaa gcaggaagag tagagaatcg catcttccat
     2101 ccccaggaat gccgccagtt tttgttcaag ctctttatgg ctgtcctgag tgccgcaaat
     2161 aaaacgcacc gaagccatgc cgaaaccgtg agaatccatt cccgcctttg ctgccgcaat
     2221 cagatccgga tgattcgcca gcccgagata gttgttggca caaaagttaa tgacgtggct
     2281 tccatcagcc acggtgatat ctgcttgctg cgcagacgta ataatgcgct cttctttaaa
     2341 caacccttcc gcccgtgcgg tttccagatc gttggttaac tgctgataaa attctccacg
     2401 cattgcgatt ctccagacag ggcaaattcc agcacatatt acccaaactt ataggtcgcg
     2461 acgagatgat gcgttaacac ttctgcaaaa ttcaggataa ataacgtgta tcttgatgtc
     2521 tctcaacacc tacagatagt agaaaaactg tacagttttg gctgagcata atgatgagtg
     2581 attttactgc attgatataa aaagaaaaaa tgatgccaaa gcatcatttt tcctatctca
     2641 gttggcatca aaacgctacc cactaatgga gggaaaccta ttttcttgaa aggaaaattc
     2701 tcagcgcctg catttttgcc cctaaagtgt tactcctgaa gcagcgaaac aaatcgaacc
     2761 attttcctct ccccagatag tttaggttga agagatcgga acgtttgtaa tagcacatga
     2821 caaaaatgcc ctggtcatcg tccacaatgt tattgtttag tgttattttt tgactttcca
     2881 gaactaattt atagaattct ttccatttat gctgtgaacc aacaattgcg cccccaataa
     2941 tatatacatg attgccaatc atgaaatcaa aagcctgttg ttgagatgta acagtaagcc
     3001 ctttttttat agtgaaaaga tgcatcttat tttcatcaaa tgggaaatcc caaattttca
     3061 atcctcgagt tacatttggc ttacggcaat agccaaaatc tatccacgca acaagaggcg
     3121 ttttcactaa acccatgttg atagctttat ttacaaaata ggccttaagg ttacatacca
     3181 aaacatactc tggtgaccag tactctgggt tttttaattg tcgaggctca agtctatttg
     3241 taaatgactc atctttttga attttttcga tacgacttct gatatatcta aatttctttt
     3301 ttatatcgat aacgataacc gttgttggtt ttccgttgcg aatcgcttca atccttggtt
     3361 tcaaatcggg agaagtgaaa ataatcatct cattttcaag tgcggccaga cgctcaaagt
     3421 aactaaaata aacatcaact gaacgagcta gtttttcacg gaacccttta ttagcagtcc
     3481 aatcccctct accaatgtca aaatatgcag tgataattgt ggtggatgat ttcattacta
     3541 ataagaccat taatataaaa aaagaattat atcataaata ttaacagaat gtatactgta
     3601 tattatccat tcaaatgatt ccgttattta tcatacatcg gaatattgat actaaagcac
     3661 tattcacatg caaaaccaac atccgccatg aaggactagc taaaacccaa actagtttgt
     3721 tgcaattagc atccttgcac ctctatgtaa agggctgaag ggattcggat gtgatggtat
     3781 gattacagac attcgtgtct gagattgtct ctgactccat aattcgaagg ttacagttat
     3841 gatcatcgtt accggcggcg cgggctttat cggcagcaac atcgttaagg ccctgaatga
     3901 taaaggcatc accgatattc tggtggtgga caacctgaaa gacggcacca agtttgtgaa
     3961 cctggtggat ctggatatcg cggactatat ggataaggaa gacttcctga tccagattat
     4021 ggctggcgaa gagttcggtg atgtcgaagc gattttccac gaaggcgcat gctcttccac
     4081 caccgagtgg gatggcaagt atatgatgga taacaactat caatactcca aagagctgct
     4141 gcactactgt ctggagcgcg aaatcccgtt cctgtatgcc tcttccgcag ccacctacgg
     4201 cggacgcacc tccgacttta ttgaatcccg cgagtacgaa aaaccgttga atgtctacgg
     4261 ttactcaaaa ttcctgtttg atgaatatgt tcgtcaaatc ctgccagaag cgaactcgca
     4321 gattgttggc ttccgctatt tcaacgttta tggaccgcgt gaaggccata aaggcagcat
     4381 ggcgagcgtc gctttccatc tcaacaccca gcttaataac ggtgaatcac cgaagctgtt
     4441 cgaaggtagc gagaacttca aacgtgactt cgtctatgtg ggcgacgtgg cagatgtaaa
     4501 cctgtggttc ctggaaaatg gcgtttccgg tatcttcaac ctcggcaccg gtcgtgcaga
     4561 atccttccag gcagtagcag atgctacgct ggcttatcac aagaaaggcc agatcgaata
     4621 cattccgttc ccggataagc tgaaaggccg ctaccaggcg ttcactcagg cagatctgac
     4681 aaatctgcgc gcggcgggtt acgacaaacc gttcaaaacc gttgctgaag gtgtaacgga
     4741 atacatggcc tggctgaatc gcgacgcata agagctctgc atgaaaatac tggtgatcgg
     4801 cccgtcttgg gttggcgaca tgatgatgtc gcaaagtctc tatcgcacgc tccaggcgcg
     4861 ctatccccag gcgataatcg atgtgatggc accggcatgg tgccgtccat tattatcgcg
     4921 gatgccggaa gttaacgaag ctatccctat gcctctcggt cacggagcgc tggaaatcgg
     4981 cgaacgccgc aaactgggtc atagcctgcg tgaaaagcgc tacgaccgcg cctacgtctt
     5041 accaaactcc ttcaaatctg cattagtgcc tttcttcgcg ggtattcctc atcgcaccgg
     5101 ctggcgcggc gagatgcgct acggtttact caacgatgta cgcgtgctcg ataaagaagc
     5161 ctggccgcta atggtggaac gctatgtcgc gctggcctat gacaaaggca ttatgcgtac
     5221 cgcacaagat ctgccgcagc cattgttatg gccgcagttg caggtgagcg aaggtgaaaa
     5281 atcatatacc tgtaatcaat tttcgctttc atcagaacgt ccgatgattg gcttttgccc
     5341 gggtgcggag tttggtccgg caaaacgctg gccacactac cactatgcgg aactggcaaa
     5401 gcagctgatt gatgaaggtt atcaggtggt tctgtttggc tctgcgaaag atcatgaagc
     5461 gggcaatgag attcttgccg ctttgaatac cgagcagcag gcatggtgtc ggaacctggc
     5521 gggggaaaca cagcttgatc aagcggttat cttgattgca gcctgtaaag ccattgtcac
     5581 taacgattct ggcctgatgc acgttgcggc ggcgctcaat cgtccgctgg ttgccctgta
     5641 tggtccaagt agcccggact tcacaccgcc gctatcccat aaagcgcgcg tgatccgtct
     5701 gattaccggc tatcacaaag tgcgtaaagg tgacgctgcg gagggttatc accagagctt
     5761 gatcgacatt actccccagc gcgtactgga agaactcaac gcgctattgt tacaagagga
     5821 agcctgacgg atgcgggttt tgatcgttaa aacatcgtcg atgggcgatg ttctccatac
     5881 gttgcccgca ctcactgatg cccagcaggc aatcccaggg attaagtttg actgggtggt
     5941 ggaagaaggg ttcgcacaga ttccttcctg gcacgctgcc gttgagcgag ttattcctgt
     6001 ggcaatacgt cgctggcgta aagcctggtt ctcggccccc ataaaagctg aacgcaaagc
     6061 gtttcgtgaa gcgctacaag cagagaacta tgacgcagtt atcgacgctc aggggctggt
     6121 aaaaagcgcg gcactggtga cacgtctggc gcatggcgta aagcatggat tggactggca
     6181 aaccgctcgc gaacctttag ccagcctgtt ttacaattgt aagcatcata ttgcaaaaca
     6241 gcagcacgcc gtagaacgca cccgcgaact gtttgccaaa agtttgggct atagcaaacc
     6301 gcaaacccag ggcgattatg ctatcgcaca gcattttctg acgaacctgc ctacagatgc
     6361 tggcgaatat gccgtatttc ttcatgcgac gacccgtgat gataaacact ggccggaaga
     6421 acactggcga gaattgattg gtttactggc tgattcagga atacggatta aacttccgtg
     6481 gggcgcgccg catgaggaag aacgggcgaa acgactggcg gaaggatttg cttatgttga
     6541 agtattgccg aagatgagtc tggaaggcgt tgcccgcgtg ctggccgggg ctaaatttgt
     6601 agtgtcggtg gatacggggt taagccattt aacggcggca ctggatagac ccaatatcac
     6661 ggtttatgga ccaaccgatc cgggattaat tggtgggtat gggaagaatc aagtagagtg
     6721 tcgttctaca agtatgtctc ttgcagattt gccagctcaa accgtttttc aaaatttaaa
     6781 tcttgaaata ataaccaata agttgacatc ggagataaga tgacctcaac attatttttc
     6841 tctctcgaga aaaaaaactg gatagcgtac tggaacagag ctctcgtatt cttattcatt
     6901 accacctatt ttttgggtgg gattacaagg tataaacatc ttattgttat tcttatgaca
     6961 ataacgacaa tcgtctatct ctgcaaacgg ccaaaacact atctctcact atttaaaaca
     7021 tttctttttg gtagtgttgc catattaact attgctgcat tgctgtcact tcttcaatcc
     7081 cctgatgcag gtgctagcat gaaggaagtt ttcaaagcta ttattgagaa tactttacta
     7141 tgcacaatag caataccggt catattgaga gacgagaaaa gagaagatgt cgaaaaaatc
     7201 gttttcttct catttattag tgcgttgggc ttacgctgtt tttctgaatt gattacctat
     7261 tataaggact atcaacaagg gataatgcca ttcgcagatt atagacaccg tagcatttct
     7321 gactcgatgg tctttttatt ccctgcattg ttaaatctct ggcttatcaa atcagcaaaa
     7381 taccgcattt cttttgtggt tctaagcgtt atttttattt ttctgatatt aggaacttta
     7441 tccagagggg cctggctttc cgtgttagtc attggattaa tatggattct gatgtttaaa
     7501 caatggaagt tactattagt aggagtaatg gtttccatca ttgcattgtc ggttattttc
     7561 acacataagg agatgaccgc aaagctaacg tataaacttc aacaaactaa tagttcttat
     7621 cgctatgcaa atggtactca aggcagcgca ctcgatctaa tattagaaaa tcctgttatt
     7681 ggttatggtt acggtaacgt tgcatataaa gatgtctata ataaacgtgt cattgattat
     7741 ccagaatgga cctttagaca atcaataggg ccacataatt ttgcgctatt catctggttt
     7801 ggcactggtt tattagggct ggtaagtctt atgatgctat actgtgcaat attgaaagag
     7861 tgtataaaaa atggcgtcaa gaataaatat cgctcaccat ataatgcata ttatataatc
     7921 ttactatctt ttataggtta ttttgttatc cgtggaaacg tagaacaaat tgaaccaaat
     7981 ttattaggcg tttacgccgg cttattatta gcgatgaaaa acaagtaaaa aataaaaaag
     8041 gctgcataat gcggcctttt tatttattca aaccaattat gaataacctc ttcgaatctt
     8101 tgagttacgc catcccaact gtagtgatca aacacaaagt cctgaccttg ctttgcaact
     8161 gctgttaatt caggattcgc taacgttttt aaaatatcgc tgcttatgga gtctgcagtc
     8221 ataggttctt ttaaatggaa tcccgtagta ttttctttga caaattccgt catcccccca
     8281 cgcgtactca ctaaaacagg cttaccagca cccatagctt caattgccac catgcaaaat
     8341 ggttcctgga actgggaagg aatgacgact aaatctgcca gtgggtaata acaatacatc
     8401 ttttcaggcg gtatgcttcc taacataatg caacgatcct tcaaacgttt tgcaatttcc
     8461 ctgacattgc gttgatatgc acctttgtca cttttactca tttctgtata atcaccaatc
     8521 acaactaatt ttaaattttt gtgagcagcg gccaattttt cgaatgattg catcaacagc
     8581 aatatgcctt tgtcagggac gatacgccca gcataaaaaa taattttctc ttcaggagag
     8641 atattaagtt cgcttctagt gatcggctga aaattagatt gataggtttc taaatcaatt
     8701 ccatttggta caatctcaat atctgcattt gctaaataag attggtaata ctttttcaag
     8761 tataaactag ggactatcat tttaacattt tgctcaagca attttggttc aaatgcatta
     8821 tgcatatgta taaccactct tgcctgagga gctagcttac gaatttgagt gtatagcttc
     8881 atgctattat gaactacaat aacactttct ttcgttatag gaaaatcatg tgcaatattc
     8941 aatattctct gtgaatatgg aagaggatca agtcttgtcc atttttgaaa taaccgctta
     9001 tatatgcgac tgaatccaac cctatgaata ctacaattat cattaacaaa ggtgtaattt
     9061 gagtatcctg gatttttgat acaaacaata cgatttggaa aattggtacg ctgagcaacc
     9121 tgatacatcc acgtttcaac agcagccgct ccgcgaggag gaattgaaaa aataggagta
     9181 accgtaaata ttattttatc aaccatctcg tatattaacc tttcataaca ttatatttaa
     9241 ttgctgtaaa aacaccatcg agaaattttt tctggtaaag caagtgttta tatttttctt
     9301 tgtgctcatg ttttttaaca gcttttttat aaggtatatt tctccatggt gatatattat
     9361 aaatattacg aaaataatct gcagaggcgt agttcgccca atcatgccat ggcttagtta
     9421 taccagtata atgtatgaag acagtgtcat cattaataaa ccgggtgtaa tattcactat
     9481 ttttttcttc aaattctgac tttatagtat aaatacaatt atattttctc ggtaatattt
     9541 tagcatgatg tagaaacatt aagtttaaaa tatcttgatc cgggtacttt aattttttta
     9601 taattgactc atctgataat agatcaaaga atttttccgt tagtctttgt tttaaccatt
     9661 cccgtaaatt aatatacatt acaccagagt taaaatagct accgttaaaa tcctcattac
     9721 acaaacgctc agcgctttta ctttgcatag catctacatc aattaccact gccccatatt
     9781 catctttaaa ctctaatgct attaactcgt tcaatgaacc cttacagacg atatcagcat
     9841 caagatataa taatgaatcc aatcgcgcag aaaaataatc aaatgaaagc aaacgataat
     9901 aaatagatac tggccagata tttgattgag gtagcgcctt caatggctca gaatcaatat
     9961 gatataattt aattactgtt tgatacttcg ttgctaattg tgataataat tcaatatact
    10021 ctttgtctaa gtaatcagcg attatgtgaa aggtaaattt gttttgttta ttcttttcaa
    10081 gaattgaagc aattgaaatc gcagccccaa cctggtagtt ctcatcaata ccccatgaga
    10141 cattaaacgt ttcccgttca tcaagtttaa cggggcgctt gtccagttca attatatctt
    10201 taaattgagt aagatgttta aaatccaatt taattaccct tcatcataat cattatttgc
    10261 tcttcaatcc tagtttgact tttacatccc tgattacttt tttaattttc tttttgaata
    10321 taagatatgt atagccaaag tctttcaatt cattttttat attatagtgc cgttcaagat
    10381 caatgcgatc ttttgctttt aaaacagcgg ttgttttttt acctgaaagg tcaattagtc
    10441 ttaaaccttt ttcggatact ataaaatttc ctttatgagg atcgccagac accattccat
    10501 gctgatgcaa ctcttttatc gattctgata attgatcctt cagatcttca gaaatttcca
    10561 aatactcatt taacccgaca ccttcaatat actcaatgag cataatataa taatgagcga
    10621 aatttaatgt tttacgttca gctagcaaaa aatagtcatt aattgattgg atgccttcac
    10681 cacgtacccg atcggtttga taaattaaat tttcataata atcttttttt atgcaagatt
    10741 ttaagaaacg ttcggtcatt ttatgctttg gcgcataaac tttaagtaca agaggtccac
    10801 gagcagtgtc aataagaatg actttagtat catcaataga acgaaaaact tttaatgttt
    10861 taaccctgca agttaaaaag tcatttagaa cttgctcata aaagggatca ttatctttta
    10921 tgaaaacttt caacccattt atcgttttat tatatatcat aatgaattat tttaacctta
    10981 aatctttaga agcatttttc tttataatac tttaaataat taaaaatgcc tgccatataa
    11041 tgcttttgtt taaatttatg ttttgcacaa taccgatact gattgctatt tacaggttta
    11101 agtaaatctt ctttactcca cggagaagct gctttggcga tcaaaaaact acgtgaaacc
    11161 ggataatcag cccactcatg ccagggtttt gtaggtccca catagtgtat aaacacggtg
    11221 tcatcattga ctggattatc aactttgtct tttaattcat agttaatact atatcgggta
    11281 ttatatttct ccgaaataaa cttcacttta ccattcaata atacattcag tacatcttga
    11341 tcaaggtggg tgattttact tacccaatct gggtcacgca acatttcaat agcttttgac
    11401 gaaatgttat ttagattcca ctcatcaata tttatcagta aaaaaccagc attgaaatat
    11461 ccagaagcta actgtggtgt agttaatacc gaggctcgat tttgccacca ttcaatatct
    11521 ctttcagcta caactgcggc aatttcatta gtagaaaatt gataatctaa gagttcttta
    11581 atactaccct tgcaagcaat atctgcatca agatatagta ttttttcatg tttatgatag
    11641 aaataatctg caattataaa tcgaaaatat gttgcgtaag tccagttttt cgtgcttggt
    11701 aatgacttca gcttatcaca attgataaca taaatattaa ttcttgaatt atattgtttt
    11761 gctaaatcag aaaaatataa tttgtcttta tcactgatat aatctgtgaa aacatgaaat
    11821 tcacaagaga tttctctatt gtttaataga atagatgcga tggctacacc acatccaaaa
    11881 agaaaatttc tgtcaatacc aaatgctata tcaagagtat ttttagatct tatattttga
    11941 taatgaaaat tatactcaaa aaggatgatg tcactatcat tgagttgaga catatttatt
    12001 ctgtatccaa aattttggca aaaataagtt acgggttaca acgattttcg aatcgttctt
    12061 tccctgattt ttgtggcttt tgcttctgct tgcgacagca gtccttgttc ctgcttgaga
    12121 atgtctttaa gcggggcggc aaaatacacc ttcataaagc gccagatatc ccgctgagtc
    12181 aggccaatat tcattgaaga aaaataaagc cctataagat ctttatcgcg ccaacgacct
    12241 ggaacgcgcg tgcgaagctg cgcccggtgt aggtcaatta ccgaaatttt taactcctct
    12301 tccttaccgg aaaaaggcaa gtgcagcagg aaatgacaga tataacagtc acgatggtta
    12361 atgcccgcag catgcatatc gcgcaccatc gtcgcgacac gcttaataag catacgcttt
    12421 acgcgaacat ctggtgggtt ggtcgcccag tcagcacagt aatcttccag actgatggtt
    12481 ggtgtcagat cttcggtaat aataaacgag gttctggtca gcggattcat gcctttttcg
    12541 ccgaatgcca ccccatacat agtatcaacg ccgacatccc gcagtcgatg aatcgcattc
    12601 cattcgcggt ctgcgcctaa tactggcatc cgcaatgaga gtaaattttt gattatctct
    12661 ttcagggtcg tgccgcgatg ccatttgaga aaatagcttt ttcccgccat ttcaaagcgc
    12721 agagtacggc gagtttccag ttcacgaaat acctcaccct gcaaggtttt aacttcctca
    12781 aaaggatctt tgccgcgcca taacgtggca aacggctctt taagttcaac catccagacc
    12841 acccgttatg atatccgccg ctttctctgg cagactgtat aaatcttgtg tatcagcata
    12901 atgtcgcgca ttttccgccc aagcctggcg caatgaagat tgcgttaacg ctttgcgtaa
    12961 aatctcattc aatgtttcct ggcggaatgg ctcagcaata gcctcgccgc aattagcgtc
    13021 gacaatataa tgcgcatagc cacaaacggc agttgttagt accggtaatc ctgcagtaat
    13081 cgcttccagc agcacaattc ccgccgcttc ctggtaggca ggatgcagta ataaatccgc
    13141 cgccgccatt aattccgaga catcgttgcg ccccgagaag aagtgaacat tactgcgcac
    13201 gccgcgtttt tctgccagtg cctcaaattt tcgcggttta tcctgcccaa caacatatag
    13261 caatgtgttg tggcgcagcg aatccggtaa cgaagcaagt gcttcaatgg aacgatcggc
    13321 acctttacgc gtgaagtctg aaccgacctg caacaataaa tattgttgtt cggttattcc
    13381 attcttctta cggaagattt cacggctatt ggctggctgc tggctatatt tacgatcagg
    13441 ataaatcccc ggtggcagaa tatgaaaacg ctccgcttca gtctgataat gtttctggaa
    13501 atcggcgatt tgcttatctg tcagcatcag cagctgtgtc ggcttgccct gttcgaaggt
    13561 tgcccgctca aaggcggcat aatggcgata acgtgacgtc aggcgataga aaaagccttt
    13621 ttcctgcgct actttctcgg cataacaaac atcagcggca taataaacgt ccagccccgg
    13681 cattttgttg aatccaacga ctttatcgac gggatgttcg cgtaaatgtt tttgcaccca
    13741 ggcaaaatac tccgcattgc gcccgtgatt ggtatgcgat ttaaccggca ctttgatcac
    13801 ttcaaataca tcagggcatt cgccttccca cgactgggta taaacccgaa catgatgacc
    13861 tcgggcggcg actgtctgag caatacgcat aaaatcgcgc tgcaaaccgc caaagggaaa
    13921 atatttatat aaacaaaaag caacgatcat aacgacgtac ctgtcgtgga ggagggcagt
    13981 aatttatcga cagcagcaat gacatctgcc gccggaataa ccgaaagata catctcattt
    14041 cggtcacgct gatcgcgcgt tggcatttcc cggtaatctc ccgcccagaa ttgaatcatg
    14101 ttatttgacc agggacgcca gaaaatatga tctgttgcgc caaacagcga tatcagcggc
    14161 gtattaactg cagcggcaat atgcgccggt gcggaatcaa cgccaataaa cagctgcgca
    14221 tgatcgatta acgcaccaag ttccgggaag gtcacctttc cagccagcgc cgttactggt
    14281 ggcgtctggc atccctgcgc aatttcattg acgcaggcca gatcgtcttt atctgggccg
    14341 gacgtcagaa cgacttcata accacgagca tgtaaggcat caatcacagc ggaaaacttg
    14401 gcgttgtccc agcatttgaa gatttgccgc gccgtaggtt ggataaccac ataattttgt
    14461 ccaacaccag cgtgatcaag ttcgcgacgc atacgtttcc agcttgcagg cgggtaactc
    14521 atggttgtct gcttcaccaa cgaatcaagt cccaatggtg tcagcacgga taagttactt
    14581 tccaccacat ttccaccctg caacggcacc aaatgggtga aacttttacg ccaaaaagca
    14641 gactgccgat gatgataatc ctgggaaatt ttcacacgag catttaataa gcgaaccagt
    14701 atagcaacca tccattgatc ggtaagattg acgataaggt catacttatt ggcacgtaat
    14761 accttgatga gatgaaaaaa gttggcaatt ttttctgagg cttttgcttt tttatttttt
    14821 atgccgtaga gcgcgttaat ctctggattt tcagacagga tcgggatggt gtcctgataa
    14881 agcagcacat cgatttttgc gtcagggtaa ttttttttca gagaactaat gacgggagta
    14941 gttaataaca tatccccatg aaaacgcatc ttaatgagca aaattcttcg aaatggctta
    15001 tccacaagta actctttttg ttgagattat ccgattaagg tgaagcagaa aaagcacgct
    15061 accgccccag gctcaacagc tacctgaata ctgataccgc actgatattg acgcgctagt
    15121 gtatcacttc tttcgcggca atccgacccg aatgattttt aagcacattg ttgattttca
    15181 ataaagaaat caatcaaata aaatcattag caaccatatt ctttcactgt tgctctgcga
    15241 ttccttcctg cacgtacagc agaaatcaat gaatcgcata aaaatacaaa aaaagtatag
    15301 atgttcaaca agaattggcg ggtaaattgc tatttaagtc aaaaacgggt aatgactcca
    15361 acttactgat agtgttttat gttcagataa tgcccgatga ccttgtcatg cagctccacc
    15421 gattttgaga acgacagt
//
LOCUS       DAJTRD010000089        14977 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTRD010000089 DAJTRD010000000
VERSION     DAJTRD010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14977
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.089"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(519..1460)
                     /gene="fabY"
                     /locus_tag="OO315_003130"
                     /gene_synonym="yiiD"
     CDS             complement(519..1460)
                     /gene="fabY"
                     /locus_tag="OO315_003130"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5891946.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1505..1942)
                     /gene="dtd"
                     /locus_tag="OO315_003131"
     CDS             complement(1505..1942)
                     /gene="dtd"
                     /locus_tag="OO315_003131"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5891947.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1939..2811)
                     /locus_tag="OO315_003132"
     CDS             complement(1939..2811)
                     /locus_tag="OO315_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5891948.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2805..3404)
                     /gene="yihX"
                     /locus_tag="OO315_003133"
     CDS             complement(2805..3404)
                     /gene="yihX"
                     /locus_tag="OO315_003133"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5891949.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(3503..4288)
                     /locus_tag="OO315_003134"
     CDS             complement(3503..4288)
                     /locus_tag="OO315_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5891950.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(4322..5218)
                     /gene="yihV"
                     /locus_tag="OO315_003135"
     CDS             complement(4322..5218)
                     /gene="yihV"
                     /locus_tag="OO315_003135"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5891951.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            5386..6282
                     /gene="yihU"
                     /locus_tag="OO315_003136"
     CDS             5386..6282
                     /gene="yihU"
                     /locus_tag="OO315_003136"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5891952.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            6306..7184
                     /gene="yihT"
                     /locus_tag="OO315_003137"
     CDS             6306..7184
                     /gene="yihT"
                     /locus_tag="OO315_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5891953.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            7201..8442
                     /gene="yihS"
                     /locus_tag="OO315_003138"
     CDS             7201..8442
                     /gene="yihS"
                     /locus_tag="OO315_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5891954.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8580..9482
                     /locus_tag="OO315_003139"
     CDS             8580..9482
                     /locus_tag="OO315_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5891955.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9681..11717
                     /gene="yihQ"
                     /locus_tag="OO315_003140"
     CDS             9681..11717
                     /gene="yihQ"
                     /locus_tag="OO315_003140"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5891956.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11763..13137
                     /locus_tag="OO315_003141"
                     /pseudo
     CDS             11763..13137
                     /locus_tag="OO315_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            13180..14583
                     /locus_tag="OO315_003142"
     CDS             13180..14583
                     /locus_tag="OO315_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5891957.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14651..>14977
                     /gene="ompL"
                     /locus_tag="OO315_003143"
     CDS             14651..>14977
                     /gene="ompL"
                     /locus_tag="OO315_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5891958.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3674 a         3856 c         3735 g         3712 t
ORIGIN      
        1 gatttaatag cgtttgcttc ggtgatttat tactcattag ttgtttttct aaattaacaa
       61 cagaataact ttcttgttta atgagattaa tattggcaac cattatagcc tccttcccac
      121 ggtttaagag caaacataca acataatgat tcataacata cagagcagta aatgaatgat
      181 actgacaatg tatgccagcc cattatcttt gcataatatt tgtgagctat agattttaaa
      241 aatatttatg aaaaaagtat ggcaccagag ggggttttga ctatcaaaaa tcgctcaaac
      301 ctgaactaat atgatgagtt aagcattatc tgcaataatt gccactcagc tctacaaata
      361 atatttaaat gctcctgaac agtgatgtag caagacacgt atggtgccaa cgaagaataa
      421 aaatttataa agttcttatt caatataacc ggaatagtca gggactgatg taaatgaccg
      481 gaatgaatga gagaggcagc accgcccctc tccataagct actcttcttc gttcccgccc
      541 tcttcatacg ggccaaatgg cttcgcgggc agaacgatat acgtgccttc aaacactgca
      601 cccggcgtct cgtcgccaaa gatttcgacc tgcatctgca cccgtgcttt tcgtccgcgc
      661 gccagacggt cgagatcgcc gcttaaggca ccaaggtcgg ctaccgcatg gggtttaccg
      721 ctaatcggtt tgctgtagcg gatatgcgca tccgccagaa taatcgttcc gccgaggtgg
      781 cgttcacgca gcatcagcca gataagcccc caaccggtga gcgtcgccag cgagaataaa
      841 ctcccggcaa acagcgtatg gtgcggattc tgattgccgg tttccggcat ggtggtgata
      901 aatttttgcc cggtatattg ttgaatacgc acgcccattt tttcactaag cgggatatgt
      961 tcgtaccacg cctgttgcag ctgcgcgcac cagtcgccgc gatgcaaaat atcatccaga
     1021 gtggcgacgg gcttaatcat caaaaaatgg cgaatcggcg tggtggttgg cgtggtgatt
     1081 tctccctgat taacaaaccc cagcttggcg aaaaactcca ccgcgtcttc acgggcgcta
     1141 caggtcacgc gcttaacgcc ttcctgacgc gccaccgact ccagggtcat cgccatcagc
     1201 gttcctaacc ctttgtcctg cacgtccggg tgaacgacca taaagcgaat ggacgcttca
     1261 ttgtcggcat taatatacaa tcggcctacc gccaccagat taccctgctc gtcgacgacc
     1321 atctggtgat gcgccatcgc atcccacgcg tcgcgttccg aaccttttgg ttgatgcagg
     1381 ggcttacgca acatttccca gcgaaagtga tagtaacgct ctaactcttc ttctgtttgt
     1441 ggaacctgaa ggtgatacat agcgatactc tctcttgttt cccgtgacaa ccctggaagc
     1501 tggctcatac ctgcaaccag aatgtcacgg ggccatcatt gaccagcgat acctgcatat
     1561 cggcagcgaa gcgtcctgtt tgcgtgttca tctcttgctg gcggcagcgt tcgacgaaat
     1621 agtcatataa cgcctctgcg cgatccggtg atgcgccttt ggagaaactt gggcgcatcc
     1681 cccgttcggt atctgcggcg agggtaaact gggaaaccac cagtacactg ccgcccgcct
     1741 gttgcacgtt gagattcatc ttgccttcgg catcgctaaa gatgcggtag ccgagcacac
     1801 gctcgcacag acggtttgct ttctgttcgt cgtcatcctt ttcgacaccc aataacacca
     1861 aaagtcccgc gccaatttcg cccgtcactt ctccctccac ggtgacgctg gcacgggtta
     1921 cgcgttgaat taatgcaatc atggttcgtc gtcttcttct tgttcagctg cttgttttag
     1981 tttgcggtat tccccgagag tgacagtaat ttccgcgcca agcaagacga tacaccatgt
     2041 ccagtagacc caaacaaaga gaatggggat caccgccagc acaccgtaaa tgagctgata
     2101 tgacgggaac atggtgatat aaagcgcgaa acctttcttt cctgcttcga acaggagtgc
     2161 ggcgacaaac gcgccgacaa tcgcgtcgcg gttaggtacg cggatggtag gaacaatgct
     2221 gtacagcaac cagaaggaga tccacgacaa cagcaacgga aaaatacgca gcacgttgtc
     2281 gatgacagta ttgagatcgc tcgcccagcg cagggagagc aaataggaac tgatcgccag
     2341 actggcccct gccagcagcg gccccagcgt taaaatcatc cagtacacgg cgaacgagta
     2401 aatttttggt cgcgctcgtt tactgcgcca gatggtattc aacgcgctat cgatggagta
     2461 catcaacaat aacgcggtga cgatcagccc gcacgcccca acggcggtca tcttgttgga
     2521 attagcaaca aattgttcga tataccgctg aataacatcg ccagtagcag gcagaaagtt
     2581 ggcaaaaata aagtgacgta actgaatgct gacgtcggaa aacatgggaa aagcggcgaa
     2641 aagcgcaaaa acaacggcaa ccagcggcac taatgagagc aacgacacat aggcaaggtt
     2701 acctgccagg gttgtcatgt tgtcctcatc aatacgttgc cagagtagtt ttagccaggc
     2761 ccatagtgga cgggtacggt gcctggcttt gtcctgaatg gtttttagca taacaccttc
     2821 gcgaaatagt ccgggatggt ggttttatct ttcaccagaa tactggtaat gcctagctga
     2881 ttagctcctt ctatattatc ggcgttatcg tcgaaaaaga ccgtatcgct gggtgaaaaa
     2941 ccttccgcct gcaaaacatg ctggtaaatt cgtgcttcag gtttgcgcat ccccagatct
     3001 tgcgacagat agatatggtc agcagcatca cgaatttccg ggtattcttc cggccagaag
     3061 gtggtatgca ggcggttggt attggaaagc accaccacgc gatgcccctg ctcacgcagt
     3121 ttatgcatga tggcgatcac ttccgggcgc agcgcaacaa acaccgcctg ccagccgtga
     3181 gagaactgct cgtagcttag cggtagagcc atctcatgac acagcgcctc tgcgaacgct
     3241 tcgtcgctaa tttccccacg ctcatgctga tgaaacgcct cccccatatg aaaactcttc
     3301 ttaagcgatg ccagcggaat acgcgttaaa tcgctccagg ctcccagcac acggttaaag
     3361 tcgatatcga caatcacatt acctaaatca aagatataga gcatttttgc ctcctttcgc
     3421 gccacaagaa attaactgta gcgggaaagg atgggtttgg ctatgtggca aggtatgaag
     3481 ccagattaac ggtcacaaaa atctacgcgt cttcctgggg aagaatgatt tcaatttggc
     3541 tgtcttgtaa gcgtgatttt agcgcagcgg gcggcagctc gtcggtaaag agcgcagtga
     3601 cctgtgccac gttaccaatt tcaaccgccg ccgaagcatg atacttagtg tgatcggcga
     3661 ccagcagaat atttctcgcg tgcgccatca tcgttttcac cacgttagct tcgtttacat
     3721 caaactccat caacgcgcca tcgctctcaa tcgccccaac gcttgttacc agataatcag
     3781 cacgaaaatc agccacaaag gacgccgctg aagggccaat gatcccgcta ttatgagagc
     3841 gcaacgtacc gccgggcacc atcacttcaa agcgcgggtt gtggtaaaga atatgcgcca
     3901 cacgcaggct gttggtgatt atccgcaaat gattatggtt aagtaacgcc cgggcaacat
     3961 gctcaacagt cgtaccaatg gtgataaata ttgttgaacc atcaggaata tagtctgcca
     4021 ccgcttcggc aatcgctttt tttttctcgg tttgcgaaac ctcacgctgc tcgaacgccg
     4081 tattaacgac gctggaagcc cgacccgcgc caccgtgatg gcgcgtaatc aggccttgct
     4141 cgcttaattt acgaatatcc cggcggaccg tctgcgtgga gacatccagc agatttgcca
     4201 gctcatcaat attcatatac ccacgctcgg cgatcagcat gaggagttgg tcgtgccgcg
     4261 ggttaccggt tagttcggta aggctcatga aaattcctcg aaaaaccatc accctggcat
     4321 tttatacaaa aagtgacaaa aaagatcggg tttgatcaca gtcagggatc ccggctcgtc
     4381 caccgggacg tgtgcatttt aacgccgcta caccgctggc gaagcggact gactccgcta
     4441 aatccccact tgttgccagc gccaccgcca aagcgccgtg aaaaacatct cccgcacctg
     4501 tggtatctac cacatcaact ttgaaggccg gttgatgctg acgcccacca ttttccagcc
     4561 agtcgcagcc tgcgctaccc tgggtcacat agacatgacc atttgtgagc gtttgtgcct
     4621 gttttagcgc actggccatc tctttcacgc ccgttaagcg cgccagaccc ggttctgaaa
     4681 aggccgcgtg atcgcttaat gccaccagct cactgatatc ctgcggcgta atgtccccgt
     4741 ccagaacggt catcacaccc gcctgacggg ccagggtgaa ggctttttta gcgccgtcgt
     4801 gccagcgtac atctgccagc acaacatccc actgagagaa atcaatttcc tccaaccact
     4861 ctgcgtcagg cagcagatcc gggctggggt agttaatgat tatccgctcg cctttggtat
     4921 ccaccatgat ggcggattgc gaagatttcg cctggttata ccgtttggtg taacgggtgt
     4981 taacgcccca ggattccagc tctgccagca ggctgttgcc cgcgtcgtca tcacctacgc
     5041 gaccaataaa atcgacctgc gcccccagcc ttgccgccgc aaccgctgcc gtcgccgctg
     5101 gcccgccacc aacttccgta taatttctcg ccacgtattt accgctctcc gtcggtaacc
     5161 cttccacgta atagatgcga tccatcacgg ttatacctac acaagcaaca cgaatcatgg
     5221 tcattcctta aacattttgg gatgcgttca ttttaatttc accaattgtt taaaaagtga
     5281 cggctgtcaa ttttttgacg ataaattaca aattcgatca aaaacagaca agcaaagcat
     5341 gtcataaatg acaaaaagtg acatcactgt attcaggaga gggttatggc agcaatcgcg
     5401 tttatcggtt taggacaaat gggttcgcca atggcgagca atttattgca gcaagggcat
     5461 caacttcgcg tctttgatgt gaacgccgaa gctgtgcggc atctggtaga caaaggtgcg
     5521 actcccgccg ccaacccggc gcaggccgct aaagatgccg aatttatcat taccatgttg
     5581 ccgaatggcg atctggtgcg caacgtgttg ttcggtgaaa acggcgtttg cgaaggctta
     5641 tctaccgatg cgctggtcat tgatatgtcc accatccatc cgctgcaaac cgataaattg
     5701 attgccgata tgcaagccaa aggcttcagc atgatggatg ttccggtagg ccgtacttct
     5761 gcaaatgcca ttaccggtac tctgttactg ctggctggcg gcaccgctga acaagttgaa
     5821 cgtgccacac cgatcctgat ggcgatgggc agtgagttga tcaacgctgg cggtccgggc
     5881 atggggatcc gcgttaagct catcaacaac tacatgagca tcgcgctcaa tgcgctttcg
     5941 gcagaagccg ccgttttgtg cgaagccctg aatcttccct tcgatgttgc cgtcaaagtg
     6001 atgagcggta ccgccgccgg taaaggtcac ttcaccactt cctggccaaa caaagtcctc
     6061 agcggagatc tttctcccgc cttcatgatc gatcttgccc ataaggatct tggcatcgcc
     6121 cttgatgtcg ccaaccagct gcatgtgcca atgccgctgg gggccgcctc acgggaggtt
     6181 tatagccagg cgcgcgcagc gggtcgcggt cgccaggact ggtccgccat tctggaacag
     6241 gtccgtgtca gtgccgggat gactgccaaa gtaaaaatgt aacgactgga taaaggaata
     6301 aatgaatgaa taagtacacc atcaacgaca ttacgcgcgc atcgggcggt tttgccatgc
     6361 tggcggtcga tcagcgcgaa gccatgcgca tgatgtttgc cgcggctggg gcacccgctc
     6421 cggtagccga tagcgtttta actgatttca aagttaacgc tgcaaaggcc ctctcgcctt
     6481 atgcctcggc gattctggta gatcaacaat tctgctaccg ccaggtggtt gagcaaaacg
     6541 cgattgccaa aagttgcgcc atgattgtcg ccgccgatga gttcattcct ggcaacggta
     6601 ttccggtcga tagcgtggtt attgaccgca aaatcaatcc gctacagatc aaacaagacg
     6661 gtggcaaagc cttaaaactg ctggtgctgt ggcgtagtga tgaagatgcg cagcaacgtc
     6721 tggatatggt gaaagagttc aacgaactgt gccactcaca cggtctggta agcatcattg
     6781 agccagtcgt ccgcccaccg cgtcgtggcg ataaattcga tcgcgaacaa gcgatcatcg
     6841 atgccgccaa agagctgggc gacagtggcg ctgacctcta caaagttgaa atgccgcttt
     6901 atggcaaagg tccgcaacaa gagcttctct gtgcttcaca acgtctgaat gaccatatca
     6961 atatgccatg ggtgatcctc tcttccagtg tcgacgaaaa actgttcccg cgtgccgtac
     7021 gcgtggcaat gacggcgggc gcatcgggat tcctggcagg ccgtgcagtc tgggcatcgg
     7081 tcgtcggttt accagacaac gagctgatgc tccgtgacgt ttgcgcaccg aaattacaac
     7141 aacttggcga tatcgtcgac gaaatgatgg ctaaacgccg ctaagatgag gataaaagga
     7201 atgaaatggt ttaacaccct aagccacaac cgttggctgg aacaggaaac cgaccgcatc
     7261 tttgattttg gtaaaaattc cgtagtgccg actggttttg gctggttagg caataaaggg
     7321 caaatcaaag aagagatggg cacccatctg tggatcacag ctcgtatgtt gcacgtttat
     7381 tccgttgctg cggcgatggg tcgacctggc gcttacgcgt tggttgatca cggtattaaa
     7441 gccatgaacg gcgcactgcg tgataaaaaa tatggcggct ggtatgcctg cgtgaatgac
     7501 gagggcgtgg tggatgcctc caaacagggc tatcaacact tctttgctct gctgggtgcc
     7561 gccagcgccg tcacaacggg tcacccggaa gcgcgcaagc tgctcgatta caccattgag
     7621 attatcgaga aatatttctg gagcgaagaa gagcagatgt gcctggagtc ctgggacgaa
     7681 gccttcagca aaaccgaaga gtaccgcggc ggcaatgcca atatgcacgc ggtggaagct
     7741 ttcttgattg tttatgacgt cactcacgac aaaaaatggc tggatcgcgc gattcgcgtg
     7801 gcttccgtga ttatccacga cgtcgccaga aataatcatt atcgcgttaa cgaacacttc
     7861 gatacccagt ggaatccgct gccggattac aacaaagata acccggcgca tcgcttccgc
     7921 gcgttcggag gtacaccagg ccactggatc gaatggggcc gtttaatgct gcacatccat
     7981 gctgccctgg aagcccgttg cgaacaacca ccagcatggc tgctagaaga tgccaaaggt
     8041 ctgtttaacg ccaccgtgcg cgatgcctgg gcacccgatg gagcggacgg gattgtttat
     8101 accgttgact gggaaggaaa accggtggtc cgcgaacgtg tacgttggcc tatcgtcgaa
     8161 gcaatgggta cggcctacgc gctctacacc gtcactggcg atcgccagta tgaaacctgg
     8221 tatcaaacat ggtgggagta ctgcattaag tacctgatgg actatgaaaa tggttcctgg
     8281 tggcaggagc tggatgcgga caataaggtc atcaccaaag tctgggacgg caaacaggat
     8341 atttatcacc tgctgcattg cctggtgatc ccgcgtatcc cgttagcgcc cggcatggct
     8401 ccagcggttg cagcgggtct gctggatatt aatgcgaaat aactgatctg aaaatagggt
     8461 gcgggttttc ccctcaccct aaccctctcc ccaaaggggc gaggggaccg tccggtgcag
     8521 tttttgggtt tgacatcagc ctcacgggag gctgtatgtc gttaattaag gtgccttgta
     8581 tgcaaatcac caatatgcat tgcagtggac agaccgtttc acttgccgcg ggggattatc
     8641 acgcgaccat tgtgaccgtc ggtgctggtc tggctgaatt aacctttcag ggttgtcatt
     8701 tagtcatccc gcacaaaccc gaagagatgc cgctggcgca tctgggtaaa gtgcttattc
     8761 cgtggccgaa ccggatcgcc aacggctgtt accgttatca ggggcaggaa tatcagttac
     8821 ccattaatga acatagttca aaagccgcta ttcacgggct gcttgcctgg cgagactggc
     8881 aaattagtga attaaccacc accagcgtta ccctgacagc ttttctgccg cccagttacg
     8941 gctatccgtt tatgctggct tcacaagttg tttattcgct caacgcacac acgggtcttt
     9001 ccgttgagat cgcaagccag aatatcggca ctgtcgcagc gccttatggc gtgggtattc
     9061 atccttattt aacctgcaac ttaacatctg ttgatgaata tctgttccag cttccggcaa
     9121 accaggttta tgccgtcgat gaacacgtaa atccgacaac gcttcaccac gttgatgagc
     9181 tggatttaaa tttcacgcag gcgaaaaaaa tagccgcgac aaaaatcgac cacaccttta
     9241 aaaccgctaa tgatttgtgg gaaatgacca ttacccatcc acagcaggcg ctttcggtaa
     9301 gcctttgttc cgatcaaccc tgggtgcagg tttatagcgg tgaaaaatta cagcgtcagg
     9361 gactggcagt tgaacctatg agttgcccgc ctaacgcctt taattcaggt atcgacttat
     9421 tattactgga accaggaaaa ccgcaccgcc tgttttttaa tatttacgga cagagaaaat
     9481 aacttgtatt gattagcagt caaaatcatt gaagaacatt tcactcgcta aaggcaacat
     9541 tatgtatctt caatttatta agttaaaaac agctcagctt ctgtaaagtt tttatatatc
     9601 agttttatga gaatcatttt acataagcct gtaataacag gaaggcaaca atctcgttct
     9661 caactctatg gagattagtt atggatacgc cacgtccaca gttattagat tttcaatttc
     9721 atcagaataa cgacagtttt accctacatt ttcaacaacg tcttatttta acccataaca
     9781 aagataatcc ttgtttatgg attggctcag gtatagcgga tatcgatatg ttccgcggta
     9841 atttcagcat taaagataaa ctacaggaga aaattgcgct taccgacgcc atcgtcagcc
     9901 agtcaccgga tggttggtta attcatttca gccgtggttc tgacattagc gccacgctga
     9961 atatctctgc cgacgatcag ggccgtttat tgctggaact acaaaacgac aaccttaacc
    10021 acaaccgtat ctggctgcgc cttgccgctc aaccagagga ccatatctac ggctgcggcg
    10081 aacagttttc ctacttcgat ctgcgcggca aaccgttccc gctatggacc agtgaacaag
    10141 gcgttggtcg caacaaacaa acctatgtca cctggcaggc cgactgcaaa gaaaatgcgg
    10201 gcggcgacta ttactggact ttcttcccac agcctacgtt tgtcagcacg cagaagtatt
    10261 actgccatgt tgataacagt tgctacatga acttcgactt tagcgccccg gaataccatg
    10321 aactggcgct gtgggaagac aaagctacgc tacgttttga atgtgctgac acatacatct
    10381 ccctgctgga aaaattaact gccctgctgg gacgccagcc agaactgccc gactggattt
    10441 atgacggggt aacgctcggc attcagggcg ggacggaagt gtgccagaag aaactggaca
    10501 ccatgcgtaa cgcgggcgtg aaggttaacg gcatctgggc gcaggactgg tctgggatcc
    10561 gtataacctc ttttggcaag cgcgtgatgt ggaactggaa gtggaacagc gaaaactacc
    10621 cgcaactgga ttcacgcatt aagcagtggt atcaggaggg cgtgcagttc ctggcctata
    10681 tcaacccgta tgttgccagc gataaagatc tctgcgaaga agcggcacaa cacggctatc
    10741 tagcaaaaga tgcctctggc ggtgactatc tggtggagtt tggcgagttt tacggcggcg
    10801 ttgtcgatct cactaatcca gaagcctacg cctggttcaa ggaagtgatc aaaaagaaca
    10861 tgattgaact cggctgcggc ggctggatgg ctgacttcgg cgagtatctg cccaccgaca
    10921 cgtacctgca taacggcgtc agtgccgaaa ttatgcataa cgcctggcct gcgctgtggg
    10981 cgaagtgtaa ctacgaagcc cttgaagaaa cgggcaagct cggcgagatc ctcttcttta
    11041 tgcgcgccgg ttctaccggt agccagaaat actccaccat gatgtgggcg ggcgaccaga
    11101 acgtggactg gagtctcgac gatggcctgg cgtccgtagt cccggcggcg ctgtcgctgg
    11161 caatgaccgg acatggtctg catcacagcg acattggcgg ttacaccacc ctgtttgaga
    11221 tgaagcgcag caaagagctg ctgctgcgct ggtgcgattt cagcgccttc acgccgatga
    11281 tgcgcaccca cgaaggtaac cgtcctggcg acaactggca gtttgacggc gatgcggaaa
    11341 ctatcgccca ttttgcccgt atgactaccg ttttcaccac cctgaaacct tatctgaaag
    11401 aggccgtcgc gctgaatgcg aagtccggtc tgccggtcat gcgcccgctg ttcctgcatt
    11461 acgaagacga tgcacatacc tattccctga aatatcagta cctgttaggc cgcgacattc
    11521 tggtcgctcc ggtacacgaa gaaggtcgca gcgactggac gctctatctg ccggaggata
    11581 actgggtaca tgcctggacg ggtgaagcgt tccggggcgg ggaagttacc gttaatgcgc
    11641 ccatcggcaa gccgccggtc ttttatcgcg ccgatagcga atgggcggca ctgttcgcgt
    11701 cgttaaacag catctaatca catccgcccg tggggaaact catgggcaaa gggagaacat
    11761 cgatgagtca catcacaacg gaagatccag caactttacg cctgcccttt aaagagaaac
    11821 tctcttacgg tattggcgac ctggcctcta acatcctgct ggatatcggt acgctttatc
    11881 ttttgaagtt ttataccgac gttctggggc tgccaggcac ctatggcggc attatctttt
    11941 tgatttcaaa attctttact gcgtttaccg atatgggtac cggcattatg ttggattccc
    12001 gacgcaagat cggtccaaaa ggtaagttcc gtccttttat tctgtatgcg tcattcccgg
    12061 tcaccttact ggcgatcgcc aactttgtcg gcacaccgtt tgatgtcacc ggtaaaacgg
    12121 tgatggccac tattctgttt atgctttacg gactgttttt cagcatgatg aactgctcct
    12181 acggcgcaat ggtcccggca atcaccaaaa accccaacga acgcgcctca ctggcggcat
    12241 ggcgtcaggg cggcgctacg ctgggcctgc tgctgtgcac ggtgggattc gtgccagtta
    12301 tgaatcttat cgaaggtaat cagaaacttg gctatatctt cgccgccacg ctgttttcac
    12361 tgttcggcct gctgtttatg tggatctgct actcaggcgt gaaagagcgt tatgtcgaaa
    12421 cccaaccaac caatccggcg caaaagcctg gcctgttgca atctttccgc gcaattgccg
    12481 gtaaccgccc actgttcatt ctgtgtattg ccaacctctg caccttaggg gcgtttaacg
    12541 tcaagctcgc cattcaggtc tattacaccc agtacgtact taacgatccc atcctgttgt
    12601 cgtatatggg atttttcagc atgggctgta ttttcatcgg cgtgttcctg atgcctggcg
    12661 cagtcagacg ttttggtaag aagaaggtct atatcggcgg cctgctgatt tgggtgctgg
    12721 gcgatctgct caactatttc ttcggcggcg gctcggtcag ctttgtggcg ttctcctgcc
    12781 tggcgttctt cggctcagcg cctgaactgg gcgctggttt ccgacaccgt cgagtacggc
    12841 gagtggcgca ccggcgtgcg ttcggaaggc acggtctaca ccggcttcac cttctttcgc
    12901 aaagtgtctc aggcgctggc tggtttcttc cccggctgga tgctgacgca aattggctat
    12961 gtgccgaacg tcgcacaggc tgaccacacc attgaagggt tacgccagtt gatcttcatc
    13021 tacccaagcg cactggcggt agtcaccatt gtggcgatgg gttgcttcta cagcctgaac
    13081 gagaagatgt atgtccgcat tgtggaagag atagaagccc gtaaacgcac ggcgtaataa
    13141 taataaacga cgccctgcgg ggcgttataa ggagtgatta tgtctgacca taatccactg
    13201 acattaaaac tgaatctgcg ggaaaaaatc gcctatggta tgggcgacgt cggttcgaat
    13261 ttaatgctct gcatcggtac tctgtatctc ctcaaacttt ataccgatga attagggatg
    13321 cctgcttact atggcgggat tatctttctg gtcgcgaagt tttttaccgc gtttaccgat
    13381 atgctcaccg gatttttact cgactcgcgg aaaaatattg ggcctaaagg caaatttcgt
    13441 ccctttattt tatatgcagc agttccggcg gcgttaattg caacgctcca gtttatcgcc
    13501 accacctttt gtttgccggt taaaacgacg attgccacca cgctgtttat gatgtttgga
    13561 ctttcataca gtctgatgaa ctgctcgtat ggtgcgatga tcccggcaat taccaaaaac
    13621 ccgaatgagc gcgcgcagct cgcggcttac cgccagggtg gtgcaaccat agggctattg
    13681 atttgtaccg tggcgtttat tccgttgcag tcgctttttt ctgactcaac cgtcggttat
    13741 gcctgtgcgg cacttatgtt ctccattggc ggctttattt ttatgatgct gtgctacaga
    13801 ggcgtcaaag agcattatgt ggacacagcg ccaaccggac ataaagccag tattctcaaa
    13861 tctttttgcg cgatatttcg taatccacca ttgctggttt tatgcattgc taacctgtgt
    13921 actctggcgg catttaatat caaactggcg attcaggtct attacaccca gtatgtgctg
    13981 aatgatatta atttattgtc gtggatggga ttcttcagta tgggatgcat cctcgtcggc
    14041 gtattactgg tgccagtaac tgtaaaatgt tttggtaaaa aacaggttta tttagctggc
    14101 atggtgctat gggcggtggg tgatatactg aattatttct ggggaagtaa ctctttcact
    14161 ttcgtcatgt tctcttgtgt cgcctttttt ggcacggcct ttgtaaacag cctgaactgg
    14221 gcactggtac cagataccgt agattacggt gaatggaaaa ccggtattcg tgccgaaggt
    14281 tctgtttata ccggttatac cttctttcgt aaaatttctg ccgcacttgc tggcttcttg
    14341 ccaggcatta tgctgacgca aattggttat gttcccaaca tcgcgcaaag cgatgcgaca
    14401 ttacagggtt tgcgtcagct catttttatc tggccttgtg cactggcaat tattgctgca
    14461 ttgacgatgg gattctttta cacactcaat gaaaaacggt ttgcattgat tattgaggaa
    14521 atcaaccaac gcaaaaataa agagatggcg acagaaaaaa aaacggcttc cgtaacgtta
    14581 taaatgtcaa tagccgctat ttccatcctg gtgggtggcg gcctccctac gtttaaaaaa
    14641 tggacttatt atgaaaaaga ttaatgcaat aattttatta tcttctctga cctctgcctc
    14701 ggtatttgct ggcgcatacg tcgaaaacag ggaggcatat aatcttgcct ccgatcaggg
    14761 cgaggttatg ctacgtgtgg gttataactt cgatatgggc gcgggtatta tgttaaccaa
    14821 tacctacacc tttcagcgag aagatgaact aaaacatggc tataatgaaa ttgaaggttg
    14881 gtatccttta ttcaaaccaa ccgataaatt aaccatccag cccggtggct taattaatga
    14941 taagagtatc agttcgggtg gtgcagtgta tctggac
//
LOCUS       DAJTRD010000090        14421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTRD010000090 DAJTRD010000000
VERSION     DAJTRD010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.090"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..132)
                     /locus_tag="OO315_003144"
     CDS             complement(<1..132)
                     /locus_tag="OO315_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5891959.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            315..458
                     /locus_tag="OO315_003145"
     CDS             315..458
                     /locus_tag="OO315_003145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891960.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO315_003146"
     CDS             complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO315_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5891961.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO315_003147"
     CDS             complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO315_003147"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5891962.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO315_003148"
     CDS             complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO315_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5891963.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO315_003149"
     CDS             complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO315_003149"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5891964.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7051..7659)
                     /locus_tag="OO315_003150"
     CDS             complement(7051..7659)
                     /locus_tag="OO315_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5891965.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7730..8815)
                     /locus_tag="OO315_003151"
     CDS             complement(7730..8815)
                     /locus_tag="OO315_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5891966.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8818..9180)
                     /locus_tag="OO315_003152"
     CDS             complement(8818..9180)
                     /locus_tag="OO315_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5891967.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9717..11630
                     /gene="mtlA"
                     /locus_tag="OO315_003153"
     CDS             9717..11630
                     /gene="mtlA"
                     /locus_tag="OO315_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5891968.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11725..12873
                     /gene="mtlD"
                     /locus_tag="OO315_003154"
     CDS             11725..12873
                     /gene="mtlD"
                     /locus_tag="OO315_003154"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5891969.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12873..13460
                     /gene="mtlR"
                     /locus_tag="OO315_003155"
     CDS             12873..13460
                     /gene="mtlR"
                     /locus_tag="OO315_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5891970.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13472..13681)
                     /locus_tag="OO315_003156"
     CDS             complement(13472..13681)
                     /locus_tag="OO315_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891971.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13965..14327
                     /locus_tag="OO315_003157"
     CDS             13965..14327
                     /locus_tag="OO315_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5891972.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEEMTGFSKTTF"
BASE COUNT         3523 a         3842 c         3624 g         3432 t
ORIGIN      
        1 aatggcgtcg gcatgaggat gattacgttt tttagcaatc aggtagggaa tatcatgtaa
       61 aataataatc ccatagaata tcaccagaaa tacaaaaata agcactccca gtgcaaaata
      121 gtccaggaac atatttccct caaagaatat aaaaaagaac aattaacgca tattatgaaa
      181 tgccatgatg cagtgcaatt tccttattta tataacgaca tattgttttt tatatacttt
      241 ctgaaatgtg ctgtctggtt tttcaacagg acgaggttta tcatattgaa atagtggaac
      301 tattggccaa actaatgaat aactccagta aaacatccac agtacagatt aagcgtatta
      361 aaccttcaat tatctaccgt ttattgctga ttggcctcgg atcaccaatg gtgatttacg
      421 gcctggttcg cccgctcacc atcgaaacgc gagattaaac tcctgacaaa acacgcccca
      481 gaaacgcact tctgttggcg tagtctgggt tattgcgcac gtaggtttaa aacgtaaatt
      541 gtaacaacgt gcgctttgtt tatgccggat gcggcgtgta cgccttatcc ggcctacggt
      601 caggttcccg taggcatgat aagacgcgta agcgtcgcat caggcaatga atacccaatg
      661 cgaccagctt cttatatcag aacagcccca acggtttatc cgagtagctc accagcaggc
      721 acttggtttg ctggtaatgc tccagcatca tcttgtgggt ttcgcgaccg atacctgatt
      781 gtttgtagcc accaaacgca gcatgtgccg ggtaagcgtg ataacagttg gtccacacgc
      841 gcccagcctg tatgccgcgc cccatcttat aggccagatt accgttgcgg ctccagacgc
      901 ccgcgcccag gccatattgc gtatcgttcg ccagctccag cgcttcttcc atcgttttga
      961 aggtggtcac cgccagcacc gggccaaaaa tctcctcctg gaacacccgc atattgttct
     1021 gaccaaacag aatcgtcggt tcgaggtagt agccgtcttt cagttcacct tccagcagct
     1081 tgcgccgccc gcctgtgagc acgtcagcgc cctctttttt accgatatca atgtagttga
     1141 ggatggtttc cagttgcccg tgagaaacct gcgcgcccat ttgcgtcacg ctgtcgagcg
     1201 ggttaccgct gcgaatgctt tcgacacggc ggatggcgcg ttccataaag cgttcgtaga
     1261 ttgactcctg cactaaggca cgactcggac aggtgcaaac ttcgccctgg ttaaaggcaa
     1321 acagtgcaaa gccttccagc gctttatcga aaaaggcatc ttcttcatcc atcacatcag
     1381 caaagaagat atttggcgac ttaccgccca actccagcgt caccggaata atgttttgcg
     1441 ttgcgtattg cataatttgt tggcccactt ccgttgagcc ggtaaacgcc actttggcga
     1501 tgcgtttcga ggtcgccaga tattcgccaa tttccccacc tgcgccattg accacgttca
     1561 ccacgcccgg cggcagtaaa tcaccgacaa tttccattag cagcagtaca gaaagcgggg
     1621 taagacgtgc gggtttcagc accacacagt tgcccgccgc cagcgcggga gccattttcc
     1681 agctcgccat cagcagcggg aagttccacg ggataatctg ccccaccacg cctaacggtt
     1741 catggaaatg ataggccacg gtttcgctat caacttcact gatcccacct tcctgcgccc
     1801 gaatacacga ggcgaaatag cggaaatggt caatcgccag cggtacatcc gcagcactgg
     1861 tttcgcgaat gggtttgccg ttatcccagg tttcagctgt cgctaacagc tcgaggtttt
     1921 gttccattcg atcggcaatc ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg
     1981 cccatttatc tttcactttg tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg
     2041 aagacgccac ttcgcacagc agctgcccgg tcaccggcgt cagattctgg taatactcac
     2101 catcggcagg ggctacccat tcaccgccaa taaagttgtc gtagcgggct tttaacttga
     2161 tggggaaacc atactcgccg ggcttaatct gtgctgaagg gggattattg gtcatgatcg
     2221 tctccttgcg gtgtgaggta taacaagggt agacgtgact ggcgaaatct tcgccagtcg
     2281 gtaacagctt tacgacagct atcacgaatt tacgggcaag gattacagag atagttgaaa
     2341 aagcggctga caatttgcca gccgttgttg gaatgatgat tacattgcgg cgcgataaat
     2401 caccacaatt tcttcgtgag ttgcctggat cgggttagta aagccacagg catctttcag
     2461 ggcattagtc gccagaaccg cgaaatcttc ttctttcacg ttcaggtcgc gtaggcctgc
     2521 cgggatatcc actttcttcg ccagttcacg gatggcgtta atgcaggctt cagcaccttc
     2581 cgcgtcgttt ttacctgtca cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc
     2641 agcggcgact ttgctgttga atacctgaac atgcggcagc agaacggcgt tacatacacc
     2701 gtgtggcagg ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat aacccagaga
     2761 agcattattg aacgccatac cggcaaggaa ctgggcataa gccattgctt cacgcgcttt
     2821 cgcattactg ccatcttcaa cggctaacgg caggttttcg gcaatcatgg tcacggcttt
     2881 cagtgcacaa gcgtcagtga tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg
     2941 cgttaaggca tccatacccg ttgcggcggt cagtgacttc ggcataccaa tcatcagaga
     3001 ggagtcattg acagaaagca gcggagtcac gtgcttatca acaatcgcca ttttgttatg
     3061 acgcgcttcg tcagtgatga tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt
     3121 attgatggcg atcatcggca gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg
     3181 aatatcgccg ccattggctg ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc
     3241 accgcctaag gagatcacgc tatcgcaatt attttcttta agtaatttca aacctgcggc
     3301 gacgttttcc gtggtggggt taggttgggt gccatcataa ataacgctaa aaatattgcg
     3361 ttcttccagt gctttttgca catcacccgc catacctaat ttcgttaaca tattgtcagt
     3421 gacaattaag gtacgggtaa atccataatc tgccatcata ttcattgcat cagtcaatga
     3481 atcagcgccg atgacattca cagaaggaat aaagaaagtt gaagatgcca taacacactc
     3541 ctgaaagtgt aaaaaagggt gggcagctta ctgaggattt tcattcgaaa acatgatcat
     3601 tattattaat tatcaaaatt aatctaataa aaagcacatt gtattcatta aacaatgtgc
     3661 tttatttaaa atcataagaa atttgtatta atttatttta aaaattaatt aatcatttcc
     3721 ttatttttcc ggaaataata atgcgtcgcg caataaatga tcatttccac gacgacgcgt
     3781 aaagccaatg cggtcaaaat attccagaat ctgaattgcc agctttcggc ctacgcctaa
     3841 gcgatcgcgg aaatccgccg cgcaggttga accacactcc tgatcgagat cgcggatcat
     3901 attggcaaat tcgacaatcc gatcgttacg gtaataacga tctttgacga tcgcggtaat
     3961 tattccttgc tgcgccgcct ggcgtagcgt caggcgcatt gcctgctcgt cggtacccgt
     4021 ctcttttgcc agatcacgca cccaccacgg ctcgtcacca aacagcggct ctgctttttg
     4081 ccagatggcc tgctgctctt cgctgaagcc cgctttgtga tccggcagat gcagccaacc
     4141 gtgatgactg aggatgtcgc cgctttcgcg catcttttca atcagcaaca gtactagcgc
     4201 ttcatcttcc attggcaacg ccatacgtcg cagacgttcg cgcccaggcc caggttcatc
     4261 gcgatgttgc tcatgataag tcgctaatgt gtcgagaatt ttccgttgcc agcgagcggc
     4321 aaccggcgca ttcaacaagc tataaccagc ctgaatatag ccaggctgtt gcagcagttc
     4381 gcgcatccct tcgccgttga gctggcgcgc ccaagcgaaa tcaggaagat taaccgcgcc
     4441 gcgttgcagg tgaacggcta acgcttcgct atcattctgt gccctggcga gggaagccag
     4501 ccattgcaga tactctggtt tacgtttacc gcgacgcggc gggttaagca tcacgacgcg
     4561 cgctccggcc agcgtgttgc gggcagagat atcgcgcaat accaggcggt cgttatctgc
     4621 cagccataac ggggtgtcga agaccagttc agcaaggtta tcttccagca gtgaaacgcg
     4681 tcccgtgacg tggctggcgg cgtggtgaat atgcagcggc tgccactggg tcagcggtgc
     4741 atgggtttga agctcgacaa tcacccgtgt gaacggctct ggcggcacat cggcaagcag
     4801 ccagtcgcca cggttaatct gctctttttc cgcatcaccc gcgatgtcaa gcgcgatacg
     4861 ctgcccggca ttggcggttt ctgttggctg gttttgcgca tgcagcgcac gtacacgcat
     4921 cggtttattt acaccagtca gccagagtga atcgcctacc ttcacttccc cgcttaacgc
     4981 cgtaccggtg acgaccagcc cggcaccttt tacggtaaat gcgcggtcaa tcgcgaggcg
     5041 gaaactatgt tggctggcgt gctcgcgttc cggcaactga agcagatgct cgcgcagggc
     5101 atcaattccc cgaccttcgg ttgctgcggt gataaacagt tttgcctcag caaaaccgta
     5161 ttcccgcaga acctccttta cctggcgttc aacctcatca acacgcgctt cgtccacgcg
     5221 atcggctttg gtcagcgcca ctgtcagcat cgggttaccg gtcagctgca aaatcgccag
     5281 atgctcacgg gtctgtgcca tcacgccgtc atcgcacgcc accaccaaca gcgcgtgatc
     5341 gataccacca acgcccgcca gcatgttgga aagaaacttt tcatgaccgg gaacgtcgat
     5401 aaaaccaggc acgcgaccat ccggctgcgg ccagtaggca tagccgagat cgatggtcat
     5461 gccgcgcttt ttttcttccg gcagacggtc agcatttacg ccagtaatcg cctgcaataa
     5521 ggttgttttg ccgtggtcaa cgtgtccggc agtcgcaata atcatttcaa caacatctcc
     5581 aaaaaccgtt gctcatcttc aaggcagcgt aaatccagcc acaatcgtcc gtcataaata
     5641 cgaccaatca ccggcactgg caattcacgc cagcgggcgg ctaatgactc aaggtggcta
     5701 ccgcgtccat catggggtgt aaacgttaat gccgcgctcg gcaggcgatc aaccggcagc
     5761 gaaccactgc caatctgcga aagacatggc ataacctgta ccgcaaactc cgcgccgtaa
     5821 tgtgcgacaa ggggagcctg taaacgttgt gcctggattt gaatgacctc tgcgctgcgg
     5881 gtaagcaggc gcagggtcgg taatttttca ctcagagctt cagggtgtaa ataaagacgc
     5941 aacgtggctt ccagcgccgc gagggtcatt ttatccgcgc gtaatgcacg cttcagcggg
     6001 tggctttgca ggcgggcgat catctctttt ttaccaacga taatcccggc ctgcggcccg
     6061 cctaacaact tgtcgccgga gaagctcacc agactgacgc ccgccgcaat caactcctgc
     6121 ggcattggct ctttcggcaa accgtactgg ctaagatcga ccagcgagcc actgcctaaa
     6181 tcagtcacca ctggaacatc cagctctttg ccgagcgcca ccagttcagc ttcgtctatc
     6241 gctttggtga acccctgaat gctgtagtta ctggtatgta ctttcatcaa cagtgcggta
     6301 ttttcattca ccgcctgacg ataatcattc gcgtgcgtgc ggttggtggt ccctacttcg
     6361 tgtagggtgc agcctgcctg acgcataaca tcgggaatac gaaacgcgcc gccaatctcc
     6421 accagttcgc cgcgagatac caccacctct ttcccgctgg cagtggccgc caacatcaat
     6481 aacaccgccg ccgcattgtt attgacgata caggcatctt ccgcccccgt aatacggcac
     6541 agcagctgcg ccagcgcccg atcgcgatgt ccgcgtccgg cgtcgtccag atcgtactcg
     6601 agggtcactg gcgaacgcat agcctgcgca acggcttcca ccgcggcttc cgcctgtaaa
     6661 gctcgcccaa ggttggtatg cagcacggtt cccgtcaggt tgatcaccgg acgcagcgcg
     6721 ctctgcgcct ctttcgtcaa ccgggcatcg acttcttgcg cccagttttc acaccacgca
     6781 ggcagcgtct ggctgccacg aatcacttct cgcgcttcgt cgagcatctg acgcaacaat
     6841 tccaccacgc gggtgtgacc ataagtatca cgcaaagaaa ggaaggagct atcgcgcaat
     6901 aagcgatcaa tagccggaag ttgactatag agggaacgcg tttcggttgt cataggaaac
     6961 ctggctgatc aaggccctct cacacggaga agggcgttta acataaccac ggattgtaac
     7021 gtgagatggg tcaggaggac atatcgcgca tcaagccttt ggcggttcgg tgcgagcaaa
     7081 actttcgcgg ctaaacaggt tttcgaccag tttgactaag tgtgggcggt cgacacacca
     7141 acctggcgca acgcggcgga aattgagata tccgacagca caggcaatgg cgatagtcgc
     7201 cagattgacc gtatcggttt tgagtgtgcc atcgaccaga tatccttcca gcacatccag
     7261 actgcggttg attttctccc gctggcgtaa caattcatct tcagactgct gcgccgccgg
     7321 acgcgcctgt tcacgcaccg ataccagccc ggcatccata atgccatccg ccagcgcctc
     7381 aattttgcgc acccgcaacg actccagcgg atcgcgcggc aacatcgccg gagcgacatt
     7441 cattaactca atatattcag cgatgatcgg cgaatcaaac cagcattcgc cctcttcggt
     7501 caccagcacc ggcactttcc ctaacgggtt aaattgcgcc acgccgttat ccgcgttata
     7561 gggcagttca ttaataaatt cgaaagttat gcccttttct aacaacagaa taaaaagttt
     7621 gcgtacaaac gggctggtgt agctaccgac gagtttcatg ccgagtcctt tgtgcgagga
     7681 aaaatatcag tatggctcag gctcacgcaa aacacaggtg atataagtct caatgatcca
     7741 aataaagata atgcatccaa ctggtcattc ttagcagcac tttccgcatc accgaaacat
     7801 ggctgaaatg gtcggagtaa accgccacct gggcgtagat gccgtcgggt aaggcatcga
     7861 tatcatcgtt agggtccagt tcaatggttc ccagcacacc gtccgtgccg ggaacgaccg
     7921 ttaatgattg caataccccc tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac
     7981 tagtcaattt gccgtggaac acctgcccag gtagcgcgtt aaacaccact tccgcatcat
     8041 cgccaggttt cagacgtaac agcgagtttt gccgaaattg ggcgacaatt tgccgttttt
     8101 gctcggggat gaagaccatc accggacgca gcggcaaggc agctgcgtat gtacctgggc
     8161 ggatcagtac ctgagtgacg tagccattgc ttggtgcgcg aatgacagtc tgctcaagat
     8221 tatattttgc ttcagtaagt tgcgctctta agctcacaat ctgcgattgc tcgccgttga
     8281 ccatactgtc gagctgactc tggatctgcg cctgctccgc caccgagcct ttcactaacg
     8341 catcctgcgc gaggaaattt tgccgcgcat cgtcgatgtc acgttccgag aacggattca
     8401 ccgccgcctg gctgcctttc aggtaacgtt gataattttt aaacagacgg tcgcgctccg
     8461 ctgaaacctg ggtggtgttg gcctgcgctt cagtgagctg cgcacgcagc gtctttatat
     8521 tatgcgtcgc cgtcatcagg tcagcctgaa gtctgtcaac tcgcgcctgg taacgaaccg
     8581 ggtcgagctt aaaaagcacc tcgccctttt gaataagctg attattcttg tcagtgactt
     8641 cagtaacaat tcccgtcacc tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg
     8701 taaaagtgta agggtggttg tagttcatca acaaaatcaa accactcacc agaaacacgc
     8761 ctcccagcgc cgccgtcgcc agcgccacgt aagttaaaac aatcaataga tccataatta
     8821 gatctccggg aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg
     8881 ggaaattcgg tcagaaagtg cggcgatttc cggttcagtc gctttctcct gcgacgcaac
     8941 atgtgactgc ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa
     9001 cggccagata acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc
     9061 atgggggtga ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc
     9121 ataaaaaatc accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat
     9181 atctgcctcg gattcacgtt tatcagtgtt gttttttggc tggcagccag aagggagtca
     9241 ggctgatatt ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt
     9301 tttagaggtg atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg
     9361 tagatcacat caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca
     9421 acaaaccatc aaaacaaaaa tgtgacatta ctcacattta aatgccattt ttagcgaaaa
     9481 tcaccgcctt gtttcttttt tatacaggcg ttttgtgatg agcgtcacgt caattacctc
     9541 tctaccccct atatttatgt gactgatatc acacaaaagc ccgtcgactg gacagttaac
     9601 cgattcagtg ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa
     9661 caaaaaacct cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt
     9721 catccgatat taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc
     9781 caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt
     9841 ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctaccgc
     9901 tgctgatcgg ttataccggt ggtaagctgg taggcggcaa acgtggtggc gtagtcggtg
     9961 ccattaccac catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga
    10021 ttgcaggtcc gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga
    10081 tcaaatccgg ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc
    10141 tcgctattct ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg
    10201 ctgcgggcgt taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg
    10261 aaccggcgaa aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg
    10321 gtattcagca gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag
    10381 gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt
    10441 ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat ccacgaaatc tacttcccgt
    10501 atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt
    10561 tcacgctgac tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg
    10621 ctgtactggc gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg
    10681 cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag
    10741 aagatgatat tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca
    10801 catctccgct atctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg
    10861 ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa
    10921 ttcaggatgc aggtctgtcg cagatttcgg tcactaacag cgcgatcaac aacctgccgc
    10981 cagatgtgga cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc
    11041 cgcaggcaca gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa
    11101 ccgaacgtct cgttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc
    11161 tgaaagacag ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct
    11221 tcctcggtcg caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg
    11281 tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga
    11341 ccccgactta tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc
    11401 gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag
    11461 aagaagatga cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc
    11521 aggttatcac cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac
    11581 acaccaccag cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccta
    11641 ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta
    11701 aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt
    11761 cgtggcttta tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc
    11821 aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt
    11881 gaaaccgagc aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat
    11941 gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg
    12001 gaacgtattg ctccggctat cgccaaaggg ctggtgaaac gcaaagaaca aggtaatgaa
    12061 tccccgctga acatcatcgc ctgtgaaaac atggtacgcg gcaccacgca gctgaaaggt
    12121 catgtgatga acgccctgcc ggaagacgcc aaagcgtggg tagaagagca cgttggcttt
    12181 gtcgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg
    12241 gaagtgacgg tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca
    12301 ctgccgaaca tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa
    12361 ctcttcaccc tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat
    12421 cagaccattc gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg
    12481 gaagaaagtg gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg
    12541 tacatccaga aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc
    12601 gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc
    12661 ggtacgctgg aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg
    12721 cacttccgca gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa
    12781 ggtccgcagg cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc
    12841 gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc
    12901 gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag gccgttgaaa
    12961 accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg
    13021 tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt
    13081 acgcggtgaa gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt
    13141 ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg
    13201 cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgccttta
    13261 ccgacgacga aatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc
    13321 cgccacagtt tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac
    13381 aggctgtgcg atcaacaatg gtcctttcac tgactgagct gatttccaaa atcagcttaa
    13441 aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat
    13501 aaagccgacg ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc
    13561 cggacggaat cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg
    13621 atgccatgaa atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca
    13681 tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt
    13741 tcccggtaaa gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt
    13801 ttattatctt tttctataga tttcttaatt aatcaacgaa ttgcgtagtt tttgagcatg
    13861 ttaatgatta cgttgcagaa ctattacaat gtcatcagtt gtgaacaaag cacctggtcg
    13921 cgcatactag gggctataaa tttatcttta tcagaagcca tcacatgaaa gaagtcgaaa
    13981 aaaacgaaat caagcgtctc agcgatcgcc tggacgccat ccgccaccag caggccgatc
    14041 tgtcgctggt tgaagccgca gacaaatatg ccgagctgga aaaagagaaa gccacgctgg
    14101 aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa actgagtaaa gaagcacaga
    14161 agctgatgaa gatgccgttc cagcgcgcaa ttaccaaaaa agagcaggct gatatgggca
    14221 agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc aatgaccgcg ctggggcgcg
    14281 aaatgggcct ggaagagatg actggatttt ctaagactac gttttaagcc cctcagacga
    14341 ctggttcgat cgtatcaacc cgcatctggc ttgccttagc tggtcaatgc ggggactctc
    14401 ctgtctactc gcctaattac c
//
LOCUS       DAJTRD010000091        14175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTRD010000091 DAJTRD010000000
VERSION     DAJTRD010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.091"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..75)
                     /locus_tag="OO315_003158"
     CDS             complement(<1..75)
                     /locus_tag="OO315_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5891973.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQA"
     gene            349..3873
                     /gene="nifJ"
                     /locus_tag="OO315_003159"
     CDS             349..3873
                     /gene="nifJ"
                     /locus_tag="OO315_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5891974.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4240..5369
                     /gene="ompN"
                     /locus_tag="OO315_003160"
                     /pseudo
     CDS             4240..5369
                     /gene="ompN"
                     /locus_tag="OO315_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5510..5944
                     /gene="uspF"
                     /locus_tag="OO315_003161"
     CDS             5510..5944
                     /gene="uspF"
                     /locus_tag="OO315_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5891975.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6120..7055
                     /gene="ttcA"
                     /locus_tag="OO315_003162"
     CDS             6120..7055
                     /gene="ttcA"
                     /locus_tag="OO315_003162"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5891976.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7184..8551)
                     /gene="dbpA"
                     /locus_tag="OO315_003163"
     CDS             complement(7184..8551)
                     /gene="dbpA"
                     /locus_tag="OO315_003163"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5891977.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9029..10012)
                     /gene="zntB"
                     /locus_tag="OO315_003164"
     CDS             complement(9029..10012)
                     /gene="zntB"
                     /locus_tag="OO315_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5891978.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10267..11499
                     /gene="dgcM"
                     /locus_tag="OO315_003165"
                     /pseudo
     CDS             10267..11499
                     /gene="dgcM"
                     /locus_tag="OO315_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11520..12083)
                     /gene="smrA"
                     /locus_tag="OO315_003166"
                     /pseudo
     CDS             complement(11520..12083)
                     /gene="smrA"
                     /locus_tag="OO315_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12337..13791)
                     /locus_tag="OO315_003167"
     CDS             complement(12337..13791)
                     /locus_tag="OO315_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5891979.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGLAWNIQIMILF
                     RALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLTDNLD
                     WRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYTLEEG
                     ARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFSFSGG
                     VGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRWLLMA
                     GLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKERLKLA
                     SGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSALFFNR
                     SRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(13788..>14175)
                     /locus_tag="OO315_003168"
     CDS             complement(13788..>14175)
                     /locus_tag="OO315_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5891980.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3777 a         3568 c         3345 g         3485 t
ORIGIN      
        1 cgcctgctga acaggctcac tactacacgc cgacaacaat aaagcggcac accctaccca
       61 aaacgctgct cgcatctctt cttcctctga tcttcaagcc aaacgacacc gccataaata
      121 ataggcagca cagagggcgg cgtcgagagc tgtcctgcgc gttgccccgc atttttactt
      181 ttttatggct atttttttgc cctctgtttg atcaaaacat tcattactct gatgtggggg
      241 acacaaaagc gaaaatgcag aagaaagcca tttgctaaaa ttgaaggatt actactgggc
      301 gcgcagcaat ttcgtgcgcc cctcatttgc gcaatgtaag ggtgtcatat gattactatt
      361 gacggtaatg gcgcggttgc ttcggtgacg tttcgcatca gtgaagttat cgccatctac
      421 cctattaccc ccagttccac gatggcagaa caggctgatg cctgggccgg aaacggcttg
      481 aagaacgttt ggggagacac accacgcgtg gttgaaatgc agtcggaagc gggtgctatc
      541 gctaccgtgc atggcgcttt gcagacgggt gccctttcaa catcgtttac gtcatcgcag
      601 ggtttgctgc tgatgatccc gacgctgtac aaactggcag gcgaactgac gccatttgtc
      661 ctgcatgtag cggcacgtac cgttgccaca catgcacttt ctatttttgg cgatcattcc
      721 gacgttatgg cggtgcgcca gacgggttgc gcgatgttgt gtgcggctaa cgtccaggaa
      781 gcgcaagact ttgctctcat ttcgcacatc gcaacgctga agagccgcgt gccatttatt
      841 catttctttg atggttttcg cacgtcccac gaaatcaata aaattgtccc gctggccgat
      901 gacactattc ttgatctcat gccgcaggcc gaaattgatg ctcatcgcgc ccgggcgctc
      961 aacccggaac atccggtgat ccgcggtacg tccgccaatc ctgacactta tttccagtct
     1021 cgcgaagcca ccaatccctg gtacaacgcg gtctatgacc atgttgaaca ggcgatgaat
     1081 gatttcgctg ccgcgacagg tcgtcagtat cagccgtttg agtattacgg gcatccgcaa
     1141 gcggaacggg tgattatcct gatgggctct gccattggca cctgtgaaga agtggttgat
     1201 gaattgctaa cccgtggcga aaaagttggc gtgctgaaag ttcgtctgta ccgtcccttc
     1261 tccgccaaac acttgctgca agctctgccg ggatccgtac gcagcgtggc ggtactggac
     1321 agaaccaaag aacccggtgc ccaggcagaa ccgctctatc tggatgtgat gaccgcactg
     1381 gcagaagcct ttaataatgg tgagcgcgaa accctgcccc gtgtcattgg tgggcgctat
     1441 ggtctttcat ccaaagaatt tggcccggac tgtgtactgg cggtatttgc cgagctcaac
     1501 gcggctaaac ctaaagcacg ctttacggtt ggtatttacg atgatgtgac caatctgtca
     1561 ctgccgttgc cggaaaacac tctgccaaac tcggcgaaac ttgaagcctt gttttatggc
     1621 cttggtagtg atggcagcgt ttccgcaacc aaaaacaata tcaagattat cggtaattcc
     1681 acgccgtggt acgcacaggg ctattttgtt tacgactcca aaaaagcggg cggcctgacg
     1741 gtttctcacc ttcgcgtgag cgaacagccg attcgttccg cttatctcat ttcccaggct
     1801 gattttgttg gctgccacca gttgcagttt atcgataaat atcagatggc tgagcgttta
     1861 aaacctagcg gcattttcct gcttaatacg ccgtacagcg cagatgaagt gtggtcgcgc
     1921 ttgccgcagg aagttcaggc cgtgttaaac cagaaaaaag cgcgcttcta tgtgattaac
     1981 gcggcgaaaa tcgcccgcga atgtggcctg gcggcccgta ttaataccgt catgcagatg
     2041 gcttttttcc atctgacgca aattctgcct ggcgatagcg ccctcgcaga attgcagggt
     2101 gcgattgcca aaagttacag tagcaaaggt caggatctgg tggagcgcaa ctggcaggct
     2161 ctggcgctgg cgcgtgaatc cgtagaagaa gttccgttgc aaccggtaaa tccgcacagc
     2221 gccaatcgac cgccagtggt ttccgatgcc gcccctgatt tcgtgaaaac cgtaaccgcg
     2281 gcgatgctcg ccgggcttgg tgatgccctc cccgtttcgg cgctgccgcc agacggcacc
     2341 tggccgatgg gcaccactcg ctgggaaaaa cgcaatatcg ccgaagagat ccccatctgg
     2401 aaagaggaac tctgtaccca atgtaaccac tgcgtcgccg cttgcccaca ctcagctatt
     2461 cgcgcaaaag tggtaccgcc tgaagcgatg gaaaacgccc ctgccagcct gcattcgcta
     2521 gatgtgaaat cgcgtgatat gcgcgggcag aaatatgtct tgcaggtggc accggaagat
     2581 tgcaccggtt gtaacctgtg cgtcgaagtt tgcccggcga aagaccgtca gaatccagag
     2641 attaaagcca tcaatatgat gtctcgccag gaacatgtcg aagaagagaa aatcaattac
     2701 gatttcttcc tcaacctgcc agaaatcgac cgtagcaaac tggaacgtat tgatattcgt
     2761 acatcgcagc tgattacacc gctgtttgaa tattcaggtg cttgccccgg ttgtggcgag
     2821 acgccgtata ttaaattgct gactcagctc tatggtgacc ggatgttgat cgctaacgcc
     2881 accggctgtt cttcaattta tggcggtaac ctgccctcta caccgtatac caccgatgcc
     2941 aacggtcgtg ggccggcatg ggcgaactct ctatttgaag ataatgccga atttggcctt
     3001 ggtttccgcc tgacggtcga tcaacaccgt gtccgcgtgc tgcgtctgct ggatcaattt
     3061 gccgataaaa tcccggcgga attactgacg gcgttgaaat cagacgctac gccagaggtt
     3121 cgtcgagaac aggtcgcagc tttacgccag caactcaacg atgttgccga agcacatgaa
     3181 cttctgcgtg acgcagatgc actggtggaa aaatcaatct ggctgattgg tggcgacggc
     3241 tgggcttacg atatcggctt tggcggtctg gatcatgtat tgagtttgac ggaaaacgtc
     3301 aacattctgg tgctggatac gcaatgctat tccaacaccg gtggtcaggc gtcgaaagcg
     3361 acaccgctgg gtgcagtaac taagttcggc gagcacggca aacgtaaagc gcgtaaagat
     3421 cttggcgtca gtatgatgat gtacggtcat gtttatgtgg cgcagatttc tctcggcgcg
     3481 cagctgaacc agacggtgaa agcgattcag gaagcggaag cgtatccggg accatcgctg
     3541 atcattgctt atagcccgtg tgaagagcat ggttacgatc tggctctcag ccacgaccag
     3601 atgcgccaac tcactgctac cggcttctgg ccgctatatc gctttgatcc gcgtcgtgcc
     3661 gatgaaggca aactgccgct ggcgctggat tcacgcccgc cgtcagaagc actggaagaa
     3721 acgttacttc acgagcagcg tttccgtcgt ctgaattcgc agcagccaga agtggcagaa
     3781 cagttatgga aagatgctgc agctaatttg caaaaacgct atgacttcct agcacaaatg
     3841 gccggaaaag cggaaaaaag caacaccgat taaatgctct ggataaggat tatccaattc
     3901 taaaaaaaaa gcccggacga ctgttcgggc ttgtcttttt atatgttgga aaatcagtgg
     3961 caatgcaatg gcccaacaga aaataaaatc tgtgacaata aaggcatata acccgcgcag
     4021 aataacgtat aggaaattaa aagtataatt tttattttgt atgtattatc ttcgatacat
     4081 tcaccagact tattatcatt tcagaggaat tatttgatta aggttttact taaggcgtaa
     4141 caaatgatat ttatcggcta actgaacttc tcctttagga tgttttcact ccccgaaatg
     4201 gggataaaag gcaaataaaa taactaaagg atttattcaa tgaaaagaaa agtactggca
     4261 cttttaattc ctgccctgct cgccgcaggt gctgcacatg cagccgaagt ttataataaa
     4321 gacggcaaca aattagatct gtatggcaaa gttgatggcc tgcattattt ttctgataat
     4381 tcagcgaaag atggcgacca gagctatgcg cgtctgggtt ttaaaggcga aacccaaatt
     4441 aacgatcaac ttactggcta cggtcaatgg gaatacaata ttcaggcaaa caacactgaa
     4501 tcttcaaaaa accagtcatg gacccgtctg gcatttgccg gactgaaatt tgcagattac
     4561 ggttctttcg attacggacg taattatggc gtaatgtacg acatcgaagg ctggaccgat
     4621 atgctgcctg aatttggcgg tgactcttat accaatgcag acaactttat gactggtcga
     4681 gccaatggcg tcgcgactta tcgtaatact gatttcttcg gtctggtaaa tggtctgaac
     4741 ttcgcggtgc agtatcaagg taacaacgaa ggagccagta atggtcagga aggcaccaat
     4801 aacgaacgcg atgttcgtca tgaaaacggt gacggctggg gtctttccac aacatatgat
     4861 ttaggcatgg gctttagcgc tggtgcggca tacacctctt ctgaccgcac caatgaccag
     4921 gttaaccata ctgcggcggg tggtgataaa gcagacgcgt ggactgctgg gctaaaatac
     4981 gatgctaaca atatttacct agcaaccatg tattcagaaa cgcgtaatat gaccccgttt
     5041 ggcgacagcg attatgcagt tgcgaacaaa acccagaact ttgaagttac tagtaccagt
     5101 ttgatttttg tctgcgtccg gcagtctctt tcctgatgtc taaaggccgt gaccttcacg
     5161 ctgctggtgg tgcagacaac ccggcaggtg ttgatgataa agatctggtt aaatacgccg
     5221 atgttggcgc gacttactat ttcaataaaa acatgtccac ctatgttgac tataaaatca
     5281 acctgttgga tgaagatgac agtttctacg ctgccaacgg catctctacc gatgatattg
     5341 tcgctttagg tctggtttat cagttctaaa tcctcctgcc cgctgttatg gcgggctttt
     5401 tctgcttatt cttcctcctt tgatctaaat taaaaatgtg aactccgtca ttacacaaaa
     5461 agtgtcatct ggcgttacac tttatgcgga tactaaaaca ggaggtttta tgaacagaac
     5521 gattcttgtc cctatcgata tttcagattc agaattaact caacgcgtga ttagccacgt
     5581 tgaggcagag gcaaagattg atgatgcaga ggttcatttc ctgacggtaa taccttcact
     5641 gccctactat gcctctctgg gtttagcgta ttccgcagaa ttaccggcaa tggatgacct
     5701 gaaagcggaa gccaaatcgc aactggaaga gatcattaaa aaatttaaac tgccaaccga
     5761 cagagtgcat gtccatgttg aggaaggctc gcccaaagac cgcattctgg aattggcgaa
     5821 gaagatcccc gctcatatga tcatcattgc ttcccatcga ccggatatca ccacttatct
     5881 gctcggttcc aacgccgcag ctgtagtgcg tcacgcagag tgctccgtgc tggttgtgcg
     5941 ctgacactaa cgcccgcaca ttgctgcggg ctttttgatt catttcgcaa atgtgctgac
     6001 attttcccct ctaatccgta ccatacacgc cacagttttt atatcagatt tcttatgctg
     6061 agcgttccgg catgacgcat actcttctga tgccatataa cgaattgagt cgcttttaaa
     6121 tgtcgcaaaa tcaagaaatt agtaagaaag aacaatacaa cctgaacaaa ttacaaaaac
     6181 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
     6241 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
     6301 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
     6361 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
     6421 agattgttga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
     6481 cttgctcact gtgttctcgg ctacgtcgcg gtatccttta tcgcaccgca acggaactgg
     6541 ggacgacgaa gatcgcgttg ggtcaccatc gtgacgatat cctgcaaacg ttgttcttaa
     6601 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     6661 aacatatcgt tattcgtcca ctggcctact gccgcgagaa agatattcag cgatttgccg
     6721 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     6781 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     6841 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     6901 atttcaaagg cattacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     6961 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     7021 atgagttacg gctgaatgtg gttgaagtga aataaccagg atagcgcccg atgcgcaagc
     7081 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ctggcaatcc
     7141 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     7201 cccggcacgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     7261 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     7321 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     7381 cttttttccc gccatcgata cacaacgttg ccatttctgc ttccagcgtc acaatggaac
     7441 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     7501 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     7561 ctgtacgacc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     7621 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     7681 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg ttgctccaga tcgccgtgta
     7741 acgacaatgc actttgctct acttcattca gcgcgtcgca gacagcctgg caatcttttt
     7801 tggtattgca aaacaccaca caagaggatg gctgatgcaa gcttaataac cgttgcaaca
     7861 gaggaatttt gcctttactg gatgtctcat aaaattgttg ttcaatgggt ggcaaagcat
     7921 ctgttgagtc aatttcaatc gccaaaggat cgcgttgcac tcgtccgctg attgcggcga
     7981 tggcttccgg ccaggttgcc gaaaacagaa gcgtctgtcg agatgcaggc gcaaaacgga
     8041 tgacatcatc aatggcatcg ctaaatccca tatccagcat gcggtcggcc tcatccatca
     8101 ccaaagtatt cagcgcatcc agtgataccg tgcctttttg caagtgatcc agcaaacgac
     8161 ccggcgttgc cacgataata tgcggcgcat gttgcagcga atcacgctgc ataccgaacg
     8221 gttgaccacc gcacaacgtc aaaattttgg tatttggcag aaaacgcgcc agccgacgca
     8281 attcacctgc cacctgatcc gccagttcac gcgtaggaca cagcactaaa gcctgggttt
     8341 gaaatagcga cgcatcaatt tgctgtaaca agccgaggcc aaaagccgcc gttttgccgc
     8401 tgccggtttt cgcctgcacg cgaacatctt ttccggcaag gatcgccgga agcgcggcgg
     8461 cctgcaccgg cgtcatggtt aaataaccca actcattaag gttcgtgagt tgggcgggag
     8521 gcaaaacatt cagggtagaa aaagcggtca caatctattc tcgtggtcat cgacgcaaag
     8581 ttagcaggcg cgtatcctcg cagatctacg ctcacgatgc gacaatttaa tcggttcttc
     8641 atcgggtggt gggtcaggca tgggttgcgg gcgagggatc ggatcgggca ctggaacagg
     8701 atcgccagga atcggttcag ggacaggaat ttgcaaataa ataagtgtcg tcatatttcc
     8761 ctccggtcat tgggtggact cttaaagggt agacgctgat aaataacagg caaaaaaaag
     8821 ccgactcatc aaagtcggcg tcgtacgaat caattgtgct atgcagtaat tcaaaaaagg
     8881 aagtaagaca atatggagcg caacgcccat cgcttgacgt tgcattcacc tgcaagggag
     8941 atattgccct gaatgggtag agagtttatt gacttcgctc aaactttgcg gcgtttttgt
     9001 atacagacag ccggaaaaat tgcttttgtt acaaccattt actacggtgc aaccataaag
     9061 caacaccacc aataagaaca actaacagaa tacaaaaaat tgaaaatccg aattgccacc
     9121 cgccgccagg gatcccacca aggttgacgc caaataaccc ggtcagaaag gtactgggta
     9181 aaaagaccat tgccatcaac gacattgtat aggtacgacg agctaaattt tcctgcatca
     9241 cctgagcgat ttcatccgcc atcacgccag tccgtgctat acaggcgtcg atttcgtcaa
     9301 ggccgcgccc aaggcgatcg gcaatatcct gcatccgacg gcgttggtca tcgctcatcc
     9361 acggcaaacg ttcactggca agacgagcat aaacatcacg ttgcggtgcc atatagcgac
     9421 gcatcacaat taattgtttg cgcagcagag ccaggaatcc acgcggtgga atttgctgat
     9481 caaggagatt atcttcaagg tcgataattt tatcgtgcag ctgctcgata aattcactgg
     9541 aatgatcggt caacgcatcg cacacatcca ccagccatcc cccgcaatcg gtcggacccg
     9601 tgccctcttc cagatcgctc accacatcgt ccagcgccag cactttgcgt tgtcgggtcg
     9661 aaacaattaa ccgcccgtcc atatatacac gcatggcgac cagttgatcg gggcgttcat
     9721 cggtgctgcc gtttatacag cgcaatgtaa tcagcgtgcc ttcaccgaaa cggctgactc
     9781 ggggccgtgt gctctcgccc gccagcgcat cacgtacgtt attgggaagc agcggtgttg
     9841 tcgccagcca ttgggcgcta tcatggtgta cataattaag gtggagccag cagggatgcg
     9901 cttcatcaat cacatctgta ttttccagcg gtttaacgcc gcctctacca tccagcatcc
     9961 aggcaaatac tgcatccggg acattaacgt ccgatccctt aatcgcttcc actgtgcctc
    10021 catcatcaac gcattatttt gtagtctagc cttctggccc tgttacgcaa catctcatca
    10081 ccccattacc ctgaaatgat taataaaatt ctgtctaaat tgaatacaaa aagcaaaatg
    10141 cttttccgta tacaaaccgt gtgaagtgtt aaatagcgtc tatcattatc agaattatct
    10201 gatcatatga cgtggctttt ttgcgatcgg atagcaacaa aaattgataa aaataacggg
    10261 atctcaatga ttacgcacaa cttcaatacc ctggacttac tcaccagtcc tgtctggatt
    10321 gtttcgccct ttgaggaaca gttaatttat gccaatagcg cggcgcgact gttgatgcaa
    10381 gacctcacgt ttagtcagct acgaaccgga ccctattccg tctcctcaca aaaagaactg
    10441 ccgaaatacc tctccgatct gcaaaaccaa cacgatatta tcgaaatcct cactgttcag
    10501 cgtaaagaag aggaaacagc attaagctgt cggcttgttt tgcgagagct gacagaaaca
    10561 gaaccggtga ttattttcga aggtatcgaa gcgccggcaa cgctgggttt aaaagccagt
    10621 cgctcggcaa attatcagcg caaaaaacaa ggtttttatg cgcgcttttt tctgactaac
    10681 tctgcaccaa tgttgttgat tgacccgtca cgagatggac aaatcgtcga tgctaacctc
    10741 gccgcgctca atttctatgg ttataaccat gaaacgatgt gccagaaaca tacctaggaa
    10801 ataaatatgc ttgggcgtcg cgtcatgcct atcatgcatg aaatttcgca tttacccggc
    10861 ggtcataacc ctttgaattt tgttcataaa ctggcggatg gatctactcg tcatgtgcag
    10921 acctatgccg gaccgattga aatttatggc gacaagctca tgttatgtat tgtgcatgat
    10981 attactgagc aaaaacggct ggaggagcag ctggaacatg ctgctcacca tgacgcgatg
    11041 accggattac tgaatcggcg acagttttat cacattacgg aaccaggcca aatgcagcat
    11101 ctcgccatcg ctcaggatta cagcttgttg ctcatcgaca ccgatcgttt taaacacatt
    11161 aacgatctct atgggcattc taaaggtgat gaggtgttat gcgccctcgc ccgccccctc
    11221 gaaagttgcg ctcgcaaagg cgatttggtg tttcgttggg gaggcgaaga gtttgtctta
    11281 ttgctaccaa gaaccccact ggataccgcg ctttcgctgg ctgaaactat ccgcgtaagc
    11341 gtggcaaaag tgagtatttc gggcttacca cgctttaccg tcagcattgg tgtggcgcat
    11401 cacgaaggaa atgaaagcat cgatgaactg tttaaacgcg ttgatgatgc tttgtatcgg
    11461 gcgaaaaatg atggacgcaa ccgcgtgctg gcggcataag ccgcggatgc gtctcgagat
    11521 caacgactgc gcttagcgtg gcgctcccag ttttcttgct tcgcctgcgc cgttttacgt
    11581 agtgcgacgt aacacgcccc gctgccgcca tgatgcggta gcgcggtgca atatgcctga
    11641 acatcatcaa attcggtcag ccagcgcgcc acatagctgc ggacaatatt ggcatgtgat
    11701 ttatcttccc gccctttacc atgaataatc agcacattac gcaaaccatc cgccagggct
    11761 tgttgaatga aactgaacac cattttgcgg cactcttcca ccggctggcg caaaagattc
    11821 aggctcgcct gttgcggata tttaccactg cgcagcttat ccagcacccc atgctacaac
    11881 ccttcctgcc gaaactccag cggctgactt agcgggatga tgtcgagaaa tccggtagtg
    11941 aggaaattat caagctgcag cgtgtcgata cgctgcggcg cacgttggtt acgcgttgga
    12001 tgccagtgga catctgtagc acgtttcagc ggctggacat cttccatggc gtcaagaaac
    12061 agcgatttgt cgtcaaggtt catgtaacct cctatgcacc ttcacacgtc gtgattcaga
    12121 cgggttaatt gttcatactt actgcatcgt tcccttcaca gattgaaaca atactctact
    12181 tcatacctgt gagtatctgc gcaggcgggg cattttatac tagcctgtta gcagtatcga
    12241 acagtttagc ccgagtttaa ttcagcacag gctccgatca ggatgattta ttcatagcag
    12301 gaaccaacaa aaatgcgata aataacagac cactgattag cagaaaggta ccgaaaatgc
    12361 catagtttgc gcctccctca gcatcaattg actcaataat ttggttgaag ccagtatttc
    12421 cgatgtctgg tcacttctac tgcgattaaa aaataaagca cttctggaaa tgaagtcatt
    12481 caccgtatgg ggaacagaaa ccattttttc acccattctc gaaaaatgaa aattagttcg
    12541 attattgaga atagagccac acagggcaat accaatcgcg cccccaagat tacgtgttaa
    12601 attaaataca ccagaggcta acttcagccg ttcttttgga attcccccca gcgttaatgt
    12661 tacgatgggg gccatcgcaa actgctgcga aatccctcta attgcctgtg gaaacagtaa
    12721 ttcctgccat ccccattcgt gggttatagg ggtaaaaaga tacattgaaa acacaaatcc
    12781 gcccagcccc gccatcaaca gccatcgcag atttattttt ttacttaacc agaagtaaaa
    12841 gggaacagaa aataactgga aaatacctgt tgtgcaaacg gcaaaaccta tctcttcggc
    12901 atttaatccg cgaatctgcc ccaaaaacac aggtattaaa tatacagtag agaatatccc
    12961 aacccctcca gaaaaagaaa aaaaacatcc cagagtgaaa tttttatcct taaaagcatg
    13021 aagatccatt atcggattgg atattgtaag tgtacgcgca gcaaataaaa taaaactcac
    13081 caatgccaga actgatgtca gcaaaatagt attatcatcc agccatcccc agcgcgctcc
    13141 ttcttcaagg gtatattcca gacatcccag agtcatggcc aacaggataa tactcggata
    13201 atcagcaact ttcagtaaag agagatcagg tttatcaaaa ttaaccagaa aaggaatact
    13261 caaaactaaa tatatgccag gtaatatatt gatataaaat agccatcgcc agtcgaggtt
    13321 atcagtgagc cagcccccca gagttggccc tagcgtcggt gacagtgagg ccagcgcact
    13381 gactaccgca gcagccaacc ccaactcttt accctggtaa taaataaacg ccatcgtgaa
    13441 aactaaaggg atcatggatg cgccagcagc tccctgtaaa gcacgaaaca aaatcatgat
    13501 ctgaatattc catgcaagtc cacacgcgat actcattaaa gtaaatattc ccgcggatag
    13561 agtaaaaagc caacgagtgg aaaatacccg tgacagccac ccagagagcg gaataacaat
    13621 aatctcagca attaaatagc tggtctgaag ccaggccatt tcgtctttac ctgcggacag
    13681 accaccacct atttcattta gggatgacga tacaatttga atatcaagct gagcaataaa
    13741 taatcccata cacatactgg cgaaagcaat atgtttacgc atagacatca tctctcatca
    13801 acactgacgg tgacggacag tcccgggcga agaagttgct ttaattcctt tgcgtcgtca
    13861 aatgcaatcc gtaccgggac acgctgaaca attttggtga aattgccagt ggcattttct
    13921 atcggaatca agctgaaact ggctcctgtc gctggggaaa ggctttcaat gtgaccgtga
    13981 aacacttccc ctttgagaat atcagcacgg atctcagcct tcattcctgg cttcattccc
    14041 gctatttggt tttctttata atttgcatca acccacaact cactgactgg tactagtgaa
    14101 actaacgatg tgccgccttc cacccaactg cctgtatggg cgctgcggtt tgcgacaatg
    14161 ccatcaatcg gcgca
//
LOCUS       DAJTRD010000092        14159 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTRD010000092 DAJTRD010000000
VERSION     DAJTRD010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14159
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.092"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..2865
                     /locus_tag="OO315_003169"
                     /pseudo
     CDS             1..2865
                     /locus_tag="OO315_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            2875..6521
                     /locus_tag="OO315_003170"
                     /pseudo
     CDS             2875..6521
                     /locus_tag="OO315_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6582..6989
                     /locus_tag="OO315_003171"
     CDS             6582..6989
                     /locus_tag="OO315_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891981.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7531..8619
                     /locus_tag="OO315_003172"
     CDS             7531..8619
                     /locus_tag="OO315_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5891982.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8630..10909
                     /locus_tag="OO315_003173"
     CDS             8630..10909
                     /locus_tag="OO315_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891983.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            10902..12039
                     /locus_tag="OO315_003174"
                     /pseudo
     CDS             10902..12039
                     /locus_tag="OO315_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            12036..14066
                     /locus_tag="OO315_003175"
     CDS             12036..14066
                     /locus_tag="OO315_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5891984.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASVDAFHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVG
                     SGIAGEAKLDHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSP
                     AATRRLFSFQAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPL
                     RQPGIVALREYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEG
                     EDNLLTLSLPASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKT
                     RAWALDAETAPVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQA
                     ELLANDLTAVGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3494 a         3727 c         3626 g         3312 t
ORIGIN      
        1 taattgcttt gttattaccc gaaaaaccag aaattgcaca tgaaattgcc cagcatctac
       61 tggggcaaaa aaaattaccc tcgcttgagt ggttaaaaat agtggcgact gatgagcaca
      121 ttcttgcctc attagaaaaa tatcacgagc catatgccat ttttgatgat tactattgtg
      181 gtgcgatatg gtcagccacc gtattacagg agcaaggtgt tgcagccctg ccccgatttg
      241 ctccctatgc cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat catccgttcg
      301 cattaacact gcttatacgt gtagccgggc atactaaacg ctgtcacgat cgaatgacga
      361 aagcctgcgc tgcgttccca cacgcagcac tggcagcact ggcggaactt ctagtgcaaa
      421 aagaagagaa tagttggcgc attatgctaa tgacgatgtt tatctcgcaa ccagcactgg
      481 cagaacaggt cattccctgg ctttcgacac ccgcagttgc agtgctgaaa tcatgccagc
      541 accaactgac acagccctca aaccatgcca gcgccgatct actgccagcc atagtagtct
      601 ccccaccatg gatttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat
      661 taggcatcga gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat
      721 atatctggta ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg
      781 caaggatggg ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag
      841 ttgaggcatg gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt
      901 cacctaccgg gcattattgg cagttgggga ttttgacaac attgccgctg gaggctggag
      961 aaagcagtaa aagcatggaa tgcccttact ctatctccac ataccgatac cgaatacgcc
     1021 atgttacatt ttggactcaa aggtttacct ggtttagtta actcaattac acgctatcca
     1081 caggaagcct tgcccatcac gaattacttc gcagcgagtg agctggctcc tgccgtcgcc
     1141 cgtgccttca acaaactcaa aacgctacgc gaaaacgccc gcacctggct gttgaaatac
     1201 ccggaacatg cccttaccgg cctgctgcct gcggcgctcg gcaaagccgg tgaagcacag
     1261 gataacgccc gcgctgcctt gcgtatgctt accgaaaacg gtcatcagcc attactgcaa
     1321 gaaatcgccc ggcgttataa ccagtcggaa gtaacagacg cggtgaacgc tctgcttgcg
     1381 ctcgatccct tagataatca cccgacaaaa atccccactc ttccgacctt ttatcagcca
     1441 tcgatctgga cgcgcccggt attaaaagca aacgcccaat cactgccaga taacgccctc
     1501 ctccacctcg gcgaaatgct ccgcttccct caggaagagg ctctgtatcc gggattattg
     1561 caggtgaaag acgcctgtac cgctgactct ctggctgaat ttgcctggga tctgtttacc
     1621 gcctggcaga ccgctggcgc gccgtcgaaa gagagttggg cgttcactgc gttaggcgtt
     1681 ctcggtaacg atgacaccgc ccgcaaactg acgccattaa tacgcgcctg gcctggtgaa
     1741 tcccagcata aacgcgccac cgttgggttg gatattctcg ccgctatcgg tagtgatatc
     1801 gccctgatgc agcttaacgg cattgctcag aaactgaaat tcaaagcatt acaggagcgg
     1861 gcaaaagaaa agattgccga cattgccgag agccgcgaac tcacggtagc ggagcttgaa
     1921 gatcggttag caccggatct cggtctggat gataacggtt cgttgctgct ggattttggc
     1981 ccacgccagt tcaccgtcag ctttgatgaa accttaaaac cgtttgtgcg tgatgtttcc
     2041 ggcagccgcc tgaaagacct gccaaaacca aacaaaagcg attatgagtc acaggcgaac
     2101 gatgctgtta accgctacaa attgctgaaa aaagatgcgc gtaccgtcgc ggcccagcag
     2161 gtagcaaggc ttgaatccgc catgtgcctg cgtcgccgct ggtcgccgga aaacttccaa
     2221 ctcttcctgg ttgtgcatcc gctggttcgc cacttaaccc gccgtctgat ttggggcgtt
     2281 tatagcgccg acaaccagct actggcctgt ttccgggtag cggaagataa cagctacagc
     2341 accgctgacg atgatctttt caccctgccg gaaggcgata tctctatcgg cattcctcac
     2401 gttctggaaa tatcaccgac ggatgctgtc gcctttggtc agctttttgc cgactacgaa
     2461 ctgctaccac cgttccgtca gcttgaccgt aacagttacg ccctgacaga agccgagcgc
     2521 aacgccagcg aactgacccg ctgggcaggc agaaaatgcc cgagcggccg ggtcatgggg
     2581 ctggcaaata aaggctggat aaagggcgaa ccacaggatg gaggctggat gatcaaacct
     2641 ctcggtcgct ggtcgttaat catggaaatc gacgaaggtt ttgcggtggg tatatcacca
     2701 gccgaactta gcgctgagca gctcttaagc aagctgtggc tatgggaagg caaagcggaa
     2761 agctatggct gggggagtaa ttcaacacag gaagcgcagt tctccgtact cgatgccatc
     2821 accgccagcg agctaattaa cgatattgaa gccctgtttg aataaggaaa gagcatggac
     2881 aaggaattac cgtggctggc ggataacgcc caactggaac tgaaatataa aaaaggcaaa
     2941 acaccactca gtcatcgcaa ttggccgggc gaaccagtgc ctgttatcac tgaaagcatc
     3001 attcagacac tgggtgatga attgctacaa aaagctgaga agaaaaaaaa cattgtctgg
     3061 cgttatgaaa atttttcacc ggagtggcag tccgccatta cgcaggccat caacttgatc
     3121 ggcgaacaca aaccctcagt cccggcccgc acaatggcgg cactagcctt tatcgcgcaa
     3181 aatgacaacc aacagttgct cgacgaaatc gtccaacaag aggggctgga atatgcgact
     3241 gaggtggtga ttgcacgcca gtttattgca cggtgttatg agagtgatcc tctggtagtc
     3301 acattgcagt atcagaacga ggactatggc tatggttatc gctcagaaac ctataacgaa
     3361 ttcgatctcc gactgcgtaa acacctctct ctggcagagg aaagctgctg gcagcgttgc
     3421 gccgacaaac tcattgccgc actgccagga ataaccaaag ttcgccgccc ttttattgcg
     3481 ctgatcctcc ccgaaaaacc agaaatcgcc aatgagttgg taagccttga atgcccgcgg
     3541 actcattttc attctaagga gtggttaaaa gttgttgcta ctgaccccaa agcggtgaga
     3601 aaactcgaac gctactggag ccaggatata tttagcgatc gagaagccag ctacatgtcg
     3661 catgaaaacc acttcggcta cgcggcctgc gctgcccttt tgcgcgaaca aggactggca
     3721 gtcgttccgc gcctcgcgat atatgcccat aaagaagatt gcggcagtct gctggtacaa
     3781 attaaccatc cgcaagtcat ccgcaccttg ctgttagttg ctgataaaaa caaacccagc
     3841 ctgcaacgtg tggctaaata cagtaaaaac ttcccccatg cgacactcgc cgcactggca
     3901 gaactgctgg cgttaaaaga accaccagcc cgcccaggtt atccaatcat cgaagacaaa
     3961 aagctgcctg cacagcaaaa agctcgagat gaatactggc gtacgctgtt acaaacgctg
     4021 atggcatcgc agccacaact ggcagcagaa gtgatgccgt ggttaagtac tcaagcccgg
     4081 gcagtgctga atagttattt atcggcaccg cccaaaccag ttattgatag caccgataac
     4141 agccaaatgc ctgaaatcct cgtttcacca ccatggcgtg ggagaaagaa aaagacttcc
     4201 atacgtcact tttcgctgcc tgatcttacg cttgcaccgc aaacgtacag gtcgccgaat
     4261 aaacactgga cgccggaaca gcaaactgca attgaccatt ttagcgccat gtcttttgac
     4321 gaacgtcttg ctacgagagg agcggatatc ttcttgcggg aattaggttt tgacgttcat
     4381 agcctggaat ttaataagcg tcttcttaat ggacagttgg atgcctttga tgctcagcaa
     4441 ctgcaagaat acacatcaat aggactattg cgtacttatc acgcgcatca ggccaaatct
     4501 aaacagtacc gggaagacgc caccaatgca ttactggcac aagatagcat tgcgttgatg
     4561 ggagggtggc gaacttttca gcacggattt tactatggta atgaacagcg ttgggggatt
     4621 tggaatctgt accttatcgc gcagatgccg cgcgaaatgg cggtatcgtg ctggcaacaa
     4681 attgtcgagg ctgattttca ttacaccggg gtggaatatt tgttaagtgt gctgggtacc
     4741 gatgctttgc ctggattaaa caccgccttt gcccgacatc caaaagagat atttccgctg
     4801 ctgattcatt tcggcgcaac agaactggcc ctgcccattg cccgcgtctg gcaccttttt
     4861 gcgggccagc gtaatctggc tcgccagtgg attttacaat ggccggaaca tacggctact
     4921 gcacttatcc ctcttgtctt caccaaatct agcgataaaa gcgaagctgc attacttgcc
     4981 ctgcgtttac tttacgaaca tgggcatggc gaatggctac aaactgtagc caatcgttgg
     5041 cagcgtacag atctatggcc tgccctggaa caattactta aacagagtcc aatagagatt
     5101 tacccggcac gcattccaaa agcccctgat ttctggcatc cgcaaatgtg gcccaggccg
     5161 cgccttatca ctaataatca acctgttacc gatgacgctc tggaaattat cggcgaaatg
     5221 ctgcgcttta cccagggggg acgtttttat agcgggctgg aacaactgaa aacgttctgc
     5281 cagccacaaa cgctggcagc ttttgcctgg gatctcttca ctgcgtggca acaagctggt
     5341 gcccccgcaa aagacaactg ggcatttctg gcgttaagtc tctttggtga cgaaagcacg
     5401 gcacgggatc taacgacaca tatcctcgcc tggccacaag aaggcaaatc tgcccgtgcg
     5461 gtcagtggca tgaacatcct taccctgatg aataatgata tggcgctgat gcagctgcat
     5521 catatatcgc aacgggcgaa atcccgcccc ttacgtgata acgcggcgga atttcttcag
     5581 gtagtcgcag aaaatcgcgg gctaagccag gaagagttag cggacagatt agtcccaaca
     5641 ctgggcctta atgatccgca ggcgttgagt tttgattttg gtccccggca gtttaccgtt
     5701 cgcttcgatg aaaacctcaa cccggttatc tttgatcagc aaaacgttcg ccagaaaagt
     5761 gttccccggt tgcgcgccga tgacgatcaa ctgaaagcgc ccgaggcact ggcccgacta
     5821 aaagggttaa aaaaagatgc tactcaggtg agcaaaaacc tgctcccgcg tcttgaagct
     5881 gccctacgta ccacccgacg ctggtcgctg gcagattttc attctctgtt tgttaatcat
     5941 ccctttaccc gtctggttac ccagcgatta atatgggggg ttatccggca aatgaaccgc
     6001 gtcgtttact caacgccttt cgtgtggccg cagaggggga gttctgcaat gcgcaagatg
     6061 agccaattga cctgcctgcg gacgctctga ttggcattgc ccacccgtta gaaatgacag
     6121 cagaaatgcg cagtgaattt gcacagcttt ttgccgatta cgaaattatg ccgccttttc
     6181 gccagttgtc gcgccgcacg gtgctgctca cacctgacga gtcaaccagt aacagcctga
     6241 ctcgctggga aggtaaatcc gctaccgttg ggcaacttat gggaatgcga tacaaaggct
     6301 gggagtcagg ttatgaggac gcatttgtct atgacctggg tgagtaccgg ctggtcctta
     6361 agttttcacc cggttttaac cactacaatg ttgatagcaa agcgctaatg agcttccgtt
     6421 ctcttcgagt gtaccgtgac aataaatccg tcacttttgc cgaacttgat gtgtttgatt
     6481 tgagtgaggc gttaagcgca cctgacgtca ttttccatta aaacaaagcg ggcagtgatg
     6541 cgactgcccg ttatctacac gacttaccag cggggaaagc gatgatcgtg caaaaagagc
     6601 tggttgctat ttacgattat gaggtccctg tacctgaaga tccgttttcc ttcagacttg
     6661 agatccataa atgctctgaa ttatttacag gttccgtcta ccgactggaa cggttccggc
     6721 tacgtccaac atttcatcaa cgtgatcgag aggatgttga cccgctaata aatgatgcgt
     6781 tgatttatat aagagatgag tttattgatg agcaaaaatt acgaggcgaa tcacctgaac
     6841 ctgtaatagt aatttttaat tgtaaactac agaatatatt caaccaacaa atagaattat
     6901 atactctaga taattcaagt cgcagcacat gcaacttgaa gtatgacgag tatagcttta
     6961 ttaaaggggg gaacggtatt cccccttaga tgcgtttcaa tttgcccgtt acttcaacaa
     7021 atcaatcaac cgtaacagag ccactaatag ctccagtaca gcaatgagca cggttaactt
     7081 ttgtgtcagc gtgtccatcc gctctcctta ttaaggagca caacaccagc accttaccct
     7141 tacactgcct gtcgatactg gtttacgttt gttgagtcgc catgttgcac tccggatcag
     7201 agcactacct gcggcaccac ccgtccggta gccaggactt gaaataggtt cgatccggcg
     7261 caacttccca acagtcagga ttataaatct cattatctta attgcaacac tgtccagagg
     7321 tggttataat tcgttcgttg gaacacgatg ttgcacaggc agtggtgtta ggcctgaaaa
     7381 tagtaaataa aagaacctgc ctcaccagca gtttttttta tttactgtga tctgctttcc
     7441 agatattttt cgctcaaaca actaatgcgc caaacattta ttgcgcgtaa aatatcgttt
     7501 atttcattaa tacatttcag ggatgaatat atgtcaccgc agaataatca tctgcagcgt
     7561 ccgcctgctg ctgtgttata cgccgatgaa ctggcaaaat taaaacaaaa tgataacgca
     7621 ccttgcccgc ccggttggca gttaagtttg cctgcggccc gtgcttttat ccttggcgat
     7681 agcgcgcaaa atattagccg taaagtggtg attagtccct ccgctgtcga acgtatgtta
     7741 gtcactcttg ccaccggacg cggtttgatg ttggtggggg aacctggcac cgcaaaatct
     7801 ctcctgtctg aattactggc aacctctatt agcggcgatg ccgggttaac catccagggc
     7861 ggcgcatcta ctaccgaaga tcaaatcaag tacggctgga actacgccct gcttatcaat
     7921 catggtccat caacggaagc gctggtccct gccccgcttt accagggaat gcgcgacggc
     7981 aaaatcgtcc gttttgaaga gataacgcgt acaccgctgg aagtgcaaga ctgtctgtta
     8041 ggtatgcttt ctgacagggt gatgacggtg ccggaactca ctggtgaagc cagtcagctc
     8101 tatgcgcgag aaggctttaa tattattgcc actgccaata cccgcgatcg cggcgtcaat
     8161 gaaatgagtg ctgcgctaaa gcgtcgcttc gattttgaaa ctgtctttcc gattatggat
     8221 ttcgcccagg agctggagct tgtcgccagt gcttcggcgc gtttgctggc ccatagcggt
     8281 attccgcata aagtgcctga cgcggtactg gagttactgg tccgcacctt ccgcgatctg
     8341 cgtgccaacg gcgaaaagaa aacgtcaatg gatacactaa cagcgattat gtccaccgct
     8401 gaagccgtga acgtggccca cgccgtaggc gtccgcgcct ggtttttagc gaatcgcgca
     8461 ggtgagccag ccgacctggt ggactgcatt gccggaacca tcgtcaaaga taacgaagaa
     8521 gatcgcgctc gtctgcgccg ttacttcgaa cagcgtgtcg ccacacataa agaagctcac
     8581 tggcaggctt attatcaagc ccgccaccgc ctgccgtgag gaaagatgca tgagcgagcc
     8641 gttaattgtc ggcatccggc atcatagtcc ggcctgcgcc cggctggtga aatcgttaat
     8701 cgaaagccag cggccacgat acgtgttgat tgaaggccct gctgatttta atgaccgggt
     8761 agacgaactg tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca
     8821 ggacggtgca gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg
     8881 gcaggcgcta caagccgatc gtcgtattca ggcacaaact tacttcatcg atttgccttg
     8941 ctgggcgcag agtgaagaag aggacgattc gcctgatacg caagaggaaa gccagacctt
     9001 gctgctgcgt gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga
     9061 tgaaagccag caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg
     9121 cgactccccc ggcgatgctc taaatcgtca gcgcgaagcc tttatggccc gctggattgg
     9181 atgggcgatg cagcaaaata atggcgacgt gttagttgtc tgcggtggct ggcacgctcc
     9241 ggcactggca aaaatgtggc gcgaatgtcc gcaggtaatt aacacgccag aattgccgtc
     9301 gctggcagat gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt
     9361 gctggcagga tacctttcag gaatgcctgc cccggtctgg caaaactggt gctggcagtg
     9421 gggcttacag caggcgggag aacaactgct aaaaacggtt ctcacccgtt tgcgccagca
     9481 caagttgcct gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcactggc
     9541 acagttgcgc ggtcatacac taccgttacg cactgactgg ctggatgcca tagcaggatc
     9601 gctgattaaa gaagccctga atgcgccgtt gccgtggagc tatcgcggcg ttattcatcc
     9661 cgataccgat ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa
     9721 acttgcccct tctacgccac aaccgcctct gccaaaagat gtcacttgcg aactggaacg
     9781 taccgcaatc tctcttccgg cggagcttac cttaaatcgc tttaacccca atggactagc
     9841 gcaaagtcag gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca
     9901 gggaagcaca ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct
     9961 tagccagcat gcggcattga ttgaggccgc ctgctttggt gccacactcc tggaagccgc
    10021 acgccataaa ttagaagccg atatgctgga cgcgggtgga atcggcagca ttaccacatg
    10081 tcttagccaa gcggcgtcag cgggtctggc gtccttcagt caacaattac tggagcaact
    10141 cacattatta atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct
    10201 atatgcctta tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac
    10261 gacgttatgc gcggctatcg atcgcacgct gtggctgtgt gaatccaacg gcagaccgga
    10321 tgaaaaggag tttcacgctc acttgcatag ctggcaagcg ttgtgtcata tcctgcgcga
    10381 tctacatagc ggtgtgcaac tccccggcgt ttcgctttct gcggcggtag ccttgctgga
    10441 gcgacacagt caggcgattc atgtcctggc actggatcgc ggcgcgactc ttggtgcgct
    10501 gatgcgtctg gaacatccca acgctagtgc cgaagcggcg ctgacgatgc tggcgcagtt
    10561 atccccggca caatccggcg aggcgctgca cggtttgctg gcattagccc gtcatcaact
    10621 ggcctgtcag ccggcattta tcgccggttt cagcagtcat ttaaatcaac taagtgatgc
    10681 cgattttacc aatgccctgc ccgatttacg cgcggcaatg gcctggctac cgccacgaga
    10741 acgcgggacg ctggcgcatc aggtgcttga gcattatcaa ctggcgcaac ttcccgtttc
    10801 ggcactgcaa atgccgttgc attgtccacc gcaagccatt gcacatcatc aacaactcga
    10861 acagcaggca ctggcatcgc tgcaacactg gggagttttc catgtctgaa ctgaacgatc
    10921 ttctgaccac ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga
    10981 cgctttgtgg gctggatgac aacgcccggc agatagatca cgcgctggag tggctgtatg
    11041 ggcgcgatcc tgaacggctc cagcgtggtg aacgctctag tggattaggt ggctcaaatc
    11101 tcaccactcc tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc
    11161 ggctggaaag cgatgccgta ctgcgctacg gcattgaaga tgtggtgaca aatctcgacg
    11221 tgctggaacg tatgcagcct tccgaaagcc tgctacgcgc cgttttgcac accaaacatc
    11281 tgatgaaccc cgaagtactg gctgccgccc gcaggattgt gcgccaggtt gtggaagaaa
    11341 ttatggcgcg actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgtcgtc
    11401 gccgctcatt tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgccaacc
    11461 tgcaacactg gcacccgcaa cacggcaagt tgtatatcga attcccccgc tttaacagcc
    11521 gcattaaacg ccaaagcgag caatggcaac tggtcttact ggttgatcaa agcggatcga
    11581 tggtcgattc ggtgatccac tctgcggtga tggcggcctg tttatggcag ttacccggca
    11641 ttcgtaccca tctggtggcg tttgaccaca agcgtcgttg atctcacggc agacgttgcc
    11701 gatccggtag agttattaat gaaagtgcaa ttaggcggcg gaaccaatat cgccagtgcc
    11761 atggagtatg gtcggcaact tattgaacaa cctgcaaaaa gcgtcattat cctcgtgagc
    11821 gatttttatg aagggggttc atcatcattg ctgacgcacc aagtgaaaaa gtgtgtccag
    11881 agcggcatca aagtgctggg actggcagcg ctcgatagca cagcaacacc ttgctatgac
    11941 cgcgatacgg cccaggcgct ggttaatgtc ggcgcacaaa tagccgccat gacaccgggc
    12001 gagctggcat catggcttgc ggagaatctt cagtcatgaa ttcactacgt ccggaattat
    12061 tagaactgac accgcaggca ctgacggcgt taagcaatgc cggttttgtt aagcgcagcc
    12121 ttaaggaact ggaaaatggc aacgtcccgg aaatcagcca tgagaacagc gctttaatcg
    12181 ccaccttcag tgacggtgtc cgtacccagc tggcgaacgg ccaggcactg aaagaggctc
    12241 agtgcaccta tggggccagc ggcatgtgcc gtcatcgcgt gatgctggtg ttaagttatc
    12301 aacgactttg tgctaccgcc cagcctacgg gaaaagaaga ggaatgggat ccggcaatct
    12361 ggctggaaga actggctacc cttcccgatg ccacccgcaa acgcgcacag gcgctggtcg
    12421 ctaaaagcat caccattgag ttgttctgtg cgccgggtga aattccctct gcccgcttac
    12481 cgatgagcga tgtgcgtttt tattcccgca gcagtattcg tttcgcccgt tgtgattgta
    12541 ttgaaggcac actttgcgaa catgtcgtac tggcagtaca ggcctttgtc caggcaaaag
    12601 cgcagcaagc ggaattgact catttaatct ggcagatgcg cagcgaacac gtcacatcat
    12661 ctgacgatcc gtttgccagc gaagaaggca aaacgtgtcg tcaatatgtt caacaactaa
    12721 gccaggcatt atggctgagc ggcatcagcc agccgcttat tcactacgag gccgctttca
    12781 gccgcgcgca gcaagcagcg gaacgctgca gctggcgatg ggtgagtgaa tcgctacggc
    12841 aactgcgcgc cagcgttgat gccttccacg cccgcgccag cgttgatgcc ttccacgccc
    12901 gcgccagcca ctatcatgcc ggagaatgct tacgtcagct tgcggcatta aacagtcgat
    12961 taaattgcgc acaagagatg gcccggagcg acagtgttgg tgaagttcct cctgtgccgt
    13021 ggcgcacggt cgttggctct ggcattgccg gagaagcaaa gcttgatcat ctgcggctgg
    13081 tgtctttagg tatgcgttgc tggcaggata ttgagcatta tggtttacgc atctggttta
    13141 ccgatcccga caccggcagt attttgcacc tttcgcgcag ttggccgcga agtgaacagg
    13201 aagactcacc ggcagctacg cgtcggctgt ttagttttca ggctggcgca ctggcgggtg
    13261 ggcaaattgt ttcacaagca gcaaaacgca gtgccgatgg cgagctgctg ttagctaccc
    13321 gcaaccgctt aagcagcgtt gtaccgctgt cgcctgatgc ctggcaaatg ttgagcgcgc
    13381 cgttacgcca gccgggcatt gtggctttgc gggaatattt acgccagcgt ccccccgcct
    13441 gcatacggcc tcttaatcag gtcgataact tatttattct gccggtcgct gagtgtattt
    13501 cgctcggttg ggatagcagc cgccagacgc tggatgcgca ggtaatcagc ggcgaaggtg
    13561 aagataatct gctgacgtta tcactgccag cctcagccag cgcgccttat gccattgaac
    13621 gcatggcggc gcttttgcaa caaacagacg accccgtgtg tctggtttct ggctttgtca
    13681 gttttgttga tgggcaattg acactggaac cacaggtaat gatgacaaag actcgtgcct
    13741 gggcgctgga cgcagaaact gcgcctgtgg tggtatcact accttctgcc agcgttctgc
    13801 ctgtgccgtc tactgctcat cagttgctga tgcgctgcca ggcgttactt attcaactgc
    13861 tccataacgg ctggcgctat caggaacaga gcgctattaa tcaggcagag ttgctggcga
    13921 atgacctcac cgcggtgggt ttttatcggc tggcacatgt gttggcacaa tttcgtaata
    13981 cagaaagcga ggcacgggta gaagcaatga ataacggtgt tttgctttgc gaacaattat
    14041 tccccatgct tcagcaacaa ggatgaaata gtgcttttta ctaagagttc tactccagtt
    14101 ccggactgct cacgccacgg tattaggcat atcctatata gcccctggtg agagtcacc
//
LOCUS       DAJTRD010000093        13710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTRD010000093 DAJTRD010000000
VERSION     DAJTRD010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.093"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="OO315_003176"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="OO315_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5891985.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="OO315_003177"
     CDS             2219..2767
                     /locus_tag="OO315_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5891986.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="OO315_003178"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="OO315_003178"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5891987.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO315_003179"
     CDS             complement(3664..4854)
                     /gene="aspC"
                     /locus_tag="OO315_003179"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5891988.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO315_003180"
     CDS             complement(5039..6127)
                     /gene="ompF"
                     /locus_tag="OO315_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5891989.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO315_003181"
     CDS             complement(6728..8128)
                     /gene="asnS"
                     /locus_tag="OO315_003181"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5891990.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO315_003182"
     CDS             complement(8297..9499)
                     /gene="pncB"
                     /locus_tag="OO315_003182"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5891991.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9765..12380
                     /gene="pepN"
                     /locus_tag="OO315_003183"
     CDS             9765..12380
                     /gene="pepN"
                     /locus_tag="OO315_003183"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5891992.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO315_003184"
     CDS             complement(12587..13354)
                     /gene="ssuB"
                     /locus_tag="OO315_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5891993.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(13351..>13710)
                     /locus_tag="OO315_003185"
     CDS             complement(13351..>13710)
                     /locus_tag="OO315_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5891994.1"
                     /translation="HGIRNIDRGLVEMARSYGLSGIPLFIHVILPDALPSIMVGVRFA
                     LGLMWLTLIVAETISANSGIGYLAMNAREFLQTDVVVVAIILYALLGKLADVSAQLLE
                     RLWLRWNPAYHLKEATI"
BASE COUNT         3479 a         3485 c         3465 g         3281 t
ORIGIN      
        1 taacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgctgg tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgtgac gtggtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccgatcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcgcttt caacgccgcc gctttcggtg attaaccaat ggcagctagc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggtctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gtgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggagcaga
     1201 tggtgctatt ggcccggcaa cgcgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggca ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccagcct attcgctgct ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcacttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt accaccaact ctggcacgca aagatattct
     1501 gccgaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg ggaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg tttcgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggatg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atgatcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca attgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga gctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttccatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattacccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa tcgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attgatctgg tgtgaacaaa cccgcctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc tacacttggt tatgagcgcc tgcacaatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aaggcgcata aaaaagtcca cttttaatgc gggcctggat
     3481 ttctggcaaa gtgcgctttg tttatgccgg atgcggcacg agcgccttat ccggcctaca
     3541 aaatcgtgca aattcaaaat attgcagggg acgcgtaggc ctgataagcg tagcgcatca
     3601 ggcaatgttg cgtttgtcat cagtctcagc ccgcttttca gcgggcttca ttgtttttaa
     3661 cgcttacagc actgccacaa tcgattcgca cagcggagcc atgttatctg gtgtcatccc
     3721 ggccacattt acgcgaccag aagcaacagc atatacgcca aactcttcgc gcagacgcag
     3781 cacttgttct tttgtcagac cactgaagga gaacatgccg ttctgtttga tgataaagct
     3841 gaagtcgcgg tttgcacctt tttcctgcag cgtattgacg aacaactgac gcatacgctg
     3901 aatacgctgg cgcatatcag tcagctcttg ttcccaaatc gcacgtaatg catcgctgct
     3961 caggatggtg gcaacaacag aagcgccgtg tgctggaggg ttagagtagt tagcgcgaat
     4021 cgccgctttc atttggctga atgcgcgatc aacgatttca ctgtcggcag caaccagagt
     4081 acaagcgcca acacgctcgt tgtacaggcc aaagttttta gagtaggaac tggcaacaat
     4141 cagctcttta tgcatagccg cgaaagcgcg cagtccttca gcatcttctt ccagaccacg
     4201 ggcaaaaccc tggtaagcga agtcaaacag cggtaaccag cctttctcaa cggagagttg
     4261 tgccagtgtc tgccattgtt ccagcgtagg gtcgataccg gttgggttat ggcagcagcc
     4321 atggaacagc actacgtcgc cagcctgagc ttcgttcaga ctgttaatca gtgcatcgaa
     4381 gtcaagggtg tgattttccg catcataata agcgtattca cgaacttcca gacctgcaga
     4441 gttaaagacg ctcttatggt tcggccagct tgggttgctc acccacacac gcttaacgct
     4501 ggtatttttt gccaggaagt cggcagccac gcgtagtgcg ccagtgccac ccggagtctg
     4561 tgccgtgcga gcacgtttgt cattgatcag ggcgctacct ttaccaaaca gcagttcctg
     4621 agtgcagcga ccaaattcaa ggatgccgtc aatgccgagg taatttttgg tggtttcatt
     4681 ttcgagcaga tactgttcag cctttttcac gctggtcagt accggggttt tgcccgtctc
     4741 atctttatag acaccaatcc cgaggttaat tttgccggga cgttcatcgg cacgaaacag
     4801 atcggccagg cccagaatcg ggtcggcagg agcggcggta atgttctcaa acatgacgag
     4861 gttccattat ggttacagaa gggaagtccg ctatcagggt aacgggagat ttacaaaatt
     4921 ccaactatta ctgatgaaaa cgcaggctgt ttttgcaaga cgtgagattg ctctggaagg
     4981 cataaaaaaa acaggaccaa cgtcctgttt tttcggcatt taacaaagag gtgtgctatt
     5041 agaactggta aacgataccc acagcaacgg tgtcgtctga acctacgccc agtttgttgt
     5101 cagaatcgat ctggttgatg atgtagtcaa cataggtgga catgtttttg ttgaagtagt
     5161 aggttgcgcc cacttcaaag tagttcacca gatcaacatc accgatacct tctacgtctt
     5221 tcgctttaga tttggtgtaa gcgatggacg gacgcagacc gaaatcgaac tggtattgcg
     5281 caactaacag aacgtcttgc gttttgttgg cgaagccgct ggtgtttgta aatttattag
     5341 tgatcggcgt agcgttacgg gtttcaccgt agttcgctgc caggtagatg ttgttcgcgt
     5401 cgtacttcag accagtagcc cactgttcag cttttttacc gttgccaaga ggttgagctt
     5461 cttgcaggtt ggtacggtca gctgcaccat aagcaccaac gataccaaag ccttcgtatt
     5521 cgtagctgat agaaccgcca acaccgtcgc cgttagaacg gcgtgcagtg tcacgctcgt
     5581 ttttacccag gtactgaaca gcgaagttca ggccatcaac cagaccaaag aagttggagt
     5641 tacgataggt agcaacgccg ccaacacgac caacgaagaa gtcatcgctg tatgcagtat
     5701 caccaccaaa ttctggcagc atatcggtgt aacccagtgc atcataaacc acaccgtagt
     5761 tacggccgta atcgaaagaa ccaacgtcag cgtatttaag acccgcgaat gccagacgcg
     5821 ttttgttacc agtttgagcg tcagcgcctt cagagttgtt accctggaag ttatattccc
     5881 actgaccata accggtcaga tcggaattga tttgagtttc ccctttaaaa ccaagacggg
     5941 cataggtcat gtcgccattg ccaccgtaac tgttttcacc gttacccttg gaaaaataat
     6001 gcagaccaac agttttaccg tacagatcta ctttgttgcc atctttgtta tagatttctg
     6061 cagcgtttgc agtacctgct actaacagag cagggacgat cactgccaga atattgcgct
     6121 tcatcattat ttattaccct catggttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agtttcaaaa agaaaaaata tgtaaccaaa agtaaaaatt aaggagcttt gtgaacaccg
     6361 tcatatttcc atagaaacgt gatgatattt acagcaattt ttatctattt atatgattta
     6421 cttatattta aattaactaa acggaaattt tgtttctgat ggaaacttta tcgacctggc
     6481 acaaaatcct ctttcagaca tccagaatgc caaaaaatag tatgaaaatt gtgctatcag
     6541 ctaagaaaaa actaatccgc aataagagta cgggttattt tttgtgcgta aatgttgcaa
     6601 tgtaatattc cgtaacagga tgatcgttat ggcgggggtg caaatagatt gcttgactta
     6661 acaaacagat aacttgacag aaaggataaa aaagagccag caggcgctgg ctctttgagg
     6721 aagaaaatca gaagctggcg ttgcgcggag tacgtgggaa cggaatcaca tcacgcacgt
     6781 tttgcacgcc agttacgtaa gcaatcaggc gttcaaaacc aagaccgaaa cctgaatgcg
     6841 gaacagtacc gtagcgacgc agatcgcgat accaccagta atcttcttta ttcaggccca
     6901 tttccagcat acgctcgtcc agaacgtcca gacgttcttc acgctgggag ccaccaatga
     6961 tctcaccgat gcccggagcc agaacgtcca tagccgcaac ggttttaccg tcttcgttga
     7021 ggcgcatata gaacgcttta atatctttcg gatagttttt aaccactacc ggtgctttaa
     7081 agtgttcttc cgccagataa cgctcatgct cagaagagag atcgacgccc cagtaaaccg
     7141 ggttttcaaa cttcctgccg cagttttcga gaatggtcac tgcgtcggtg taatccacct
     7201 gcgcgaaatc ggcttcaatg aagcgttcca gacgtgaaac ggcatcttta tctacgcgtt
     7261 cagcgaagaa tttcatgtcg tctgcgcgtt cttcgagaac cgctttgaag acatatttca
     7321 gcatggcttc agccagaccc gcgatatcgt tcaggttagc aaacgccact tccggctcca
     7381 gcatccagaa ttccgccagg tgacggctgg tgttggagtt ttcagcacgg aaagtcgggc
     7441 cgaaggtata aattttggat aatgcgcaag cgtaggtttc gccgttcaac tggccagata
     7501 cggtcaggaa agactcttta ccaaagaagt ctttgtcgaa atccactttg ccctgatcgt
     7561 tacgcggcag gttttccaga tccagcgtag aaacgcggaa catttcgcct gcaccttcgg
     7621 tatcagatgc ggtaatcagt ggcgttgaaa cccagaagaa tccctgctcg ttaaagaagc
     7681 gatgcagcgc ctgcgccagc gtatggcgaa cgcgcgcgac ggcaccaatc aggtttgtgc
     7741 gcggacgcag gtgagcgact tcacgcagat actcaatgct gtggcgtttt gccgccatcg
     7801 ggtaagtgtc tggatcttca acccaaccag caacttcaac cttgctggcc tgaatttcaa
     7861 attgttgccc ctggcccggc gatgccacga ctttacccgt cacaatgacc gagcagccgg
     7921 tggtcagacg caggacgtct tcattgtaat tgggcagaga attattgatg acagcctgta
     7981 caggatcaaa gcaggaaccg tcataaacgg cgaggaagga gatgccagct tttgaatctc
     8041 ggcgggtacg tacccatccg cgtacggtga cttcgctgtc aacggctacg cggccctgga
     8101 gtacgtcggc tacaggcaca acgctcataa tattctctct gttaatagtc ggaaaaaata
     8161 aacacttgtc cacccgtaaa tgggggggat acctatgtta cctggcatct gcaatcagac
     8221 aagcagaatt cgcaaatgca gcgaaagatt tcggaattaa aggtaaaaaa gggagccgat
     8281 tagctccctg atgatattaa ctggcttttt taatatgcgg aaggtcgaac gctttgcgca
     8341 gcgcccgaac aaacgcttta tcatggcaga tagttttgcc agggctgtca gaaagtttcg
     8401 ccaccggttt accgttacac tctaccaact taatcacaat attcaggggt tttacctggg
     8461 ggatatcgca ggtcaggcga gtcccaatac caaaacttaa ttgcacgcgg gaagagaagt
     8521 ggcggtatag ctcaaccgct ttgcgtaaat ccagattgtc agagaaaacc agcgttttac
     8581 tctgtggatc aattcccagc ttttcataat gtgcaatggc tttttcaccc cattcaaccg
     8641 ggtcgccaga gtcatgacgc aggccctgat accgactagc gaactcgaca ccgaaatcac
     8701 gcaggaaagc atccatagtg atgcagtcgg ttaatgcaat gccaagttgg tcgggatact
     8761 cttccagcca ggcagcaagt gcagctcgct ggctgttggc tagatccggg ctgatttgct
     8821 gatgtgcctg gaaccattcg tgtgcctgtg ttcccatcgg cgtgagggaa agccgacgcg
     8881 ccagatcgta gttgctggtg cccacgaacc aggattcctg ttgcagacgc ttaacgatgg
     8941 tttcttgtac ttcgcgagaa aaacggcgac gggtgccaaa atccatcaga tggaagcgcg
     9001 acatatcaag accggcggtt aacgccgaga agtcgactaa tttgctttcc agcgtgtcga
     9061 gggcttgcgc aacgtcggcc tgcggtgagc gatagcgatg taccatttca ctgataaccg
     9121 ccagcaaagg aacttcccag aggatgactt cacgccacgg accgcttaag cgaatatcca
     9181 gcttgccatt atcgttggac acggtgactt gttccgggtt aaagcggaac tcgcgtaacc
     9241 agttaagata gtcggcctta aagaaaggca gggcagagag ccactgatat tcatcatcct
     9301 gcaggcgcag gtgctgcatc gcctgaatct gttcacgaat agcatcggca taaataccca
     9361 gcagatcgtc acctcggcaa cgaaactccg ccgcgacatg cacatcgtaa tagtgatgaa
     9421 acacggcttg ctgcatatgc aacttataag catctgtatc cagcaacgag tgcagaacag
     9481 gagaagcgaa ttgtgtcata ggtgcgcagt agcgtcctcg tacaggagcg tttagtacaa
     9541 taaacatctt caggaactgc tggagtatac cttgtttcgc aatttattga accccgatca
     9601 caccatatgc cacctttctg gtcgatggca ttacgcggtg tatgttataa aaatgtagca
     9661 ataaaggcgt ttgtacctga aaagatgaag attctgcata gcgcgattta cgcaacagga
     9721 atagactgaa caccagactc tataaaagat gctaaaggtt atttatgact caacagccac
     9781 aagccaaata ccgtcacgat tatcgtgcgc cggattacca gattactgat attgacttga
     9841 cctttgacct cgacgcgcaa aagacggtcg ttaccgcggt cagccaggct gtccgtcatg
     9901 gtgcatcaga tgctccgctt cgtctcaacg gcgaagacct caaactggtt tctgttcata
     9961 ttaatgatga gccgtggacc gcctggaaag aagaagaaga gggcgcactg gtcatcagta
    10021 atttgccgga gcgttttacg cttaagatca ttaatgaaat aagcctggcg gcgaatacgg
    10081 cgctggaagg gctttatcag tcaggcgatg cgctttgcac ccagtgtgaa gccgaaggtt
    10141 tccgccatat tacgtattat ctcgaccgcc cggacgtgct ggcgcgtttt accaccaaaa
    10201 ttattgccga taaaatcaaa tatcccttcc tgctttccaa tggtaaccgc gttgcgcaag
    10261 gcgaactcga aaacggacgc cattgggtac agtggcagga cccgttcccg aaaccgtgct
    10321 acctgtttgc gctggtggca ggcgactttg atgtactgcg cgataccttt accacgcgtt
    10381 ctggtcgcga agtagcactg gagctgtacg tcgatcgcgg caaccttgat cgcgcgccgt
    10441 gggcgatgac ctcgctgaaa aactccatga aatgggatga agaacgcttt ggcctggagt
    10501 atgacctcga catctatatg atcgtcgcgg tggatttctt caatatgggc gcaatggaga
    10561 ataagggtct gaatatcttt aactccaaat atgtgctggc ccgcaccgac accgccaccg
    10621 acaaagatta cctcgatatt gaacgcgtta tcggccatga atatttccat aactggaccg
    10681 gtaaccgagt gacctgccgc gactggttcc agctcagcct gaaagaaggt ttaaccgtct
    10741 tccgcgatca ggaattcagc tctgaccttg gttcccgcgc agttaaccgc atcaataatg
    10801 tgcgcaccat gcgcggattg cagtttgcgg aagacgccag cccgatggcg cacccaatcc
    10861 gcccggacat ggtcattgag atgaacaact tctacaccct gaccgtttac gagaagggcg
    10921 cggaagtgat ccgtatgatc cacaccctgc tgggcgaaga aaacttccag aaagggatgc
    10981 aactctattt cgagcgtcat gatggtagcg cagcgacctg tgacgacttt gtgcaggcga
    11041 tggaagatgc gtcgaatgtt gatctctccc atttccgccg ttggtacagc cagtccggta
    11101 cgccggttgt gaccgtcaaa gacgactaca atccggaaac tgagcagtac accctgacca
    11161 tcagccagcg cacgccagcc acgccggatc aggcagaaaa acagccgctg catattccgt
    11221 ttgccatcga attgtatgac aacgaaggta aagtgatccc gttgcagaaa ggcggtcatc
    11281 cggtgaattc cgtgctgaac gtcacccagg cggaacagac ctttgttttt gataatgtct
    11341 acttccagcc ggtgcctgcg ctgctgtgcg aattctctgc gccagtgaaa ctggaatata
    11401 agtggagcga tcagcaactg accttcctga tgcgtcatgc gcgtaatggt ttctcccgtt
    11461 gggatgcggc gcaaagtttg ctggcaacct acatcaagct gaacgtcgcc cgtcatcagc
    11521 aagggcagcc gctgtctctg ccggtacatg tggctgacgc tttccgcgcg gtgctgctcg
    11581 atgagaagat tgatccggcg ctggcggcag aaatcctgac gctgccttct gtcaatgaaa
    11641 tggctgaact gttcgatatc atagacccga ttgctattgc cgaagtacgc gaagcactca
    11701 ctcgtactct ggcgactgaa ctggcggatg agctgctggc tatttacaac gcgaattacc
    11761 agagcgagta ccgtgttgag catgaagata ttgcaaaacg cactctgcgt aatgcctgcc
    11821 tgcgcttcct tgcttttggt gaaacgcatc tggctgacgt gctggtgagc aagcaatacc
    11881 atgaagcaaa caatatgacc gatgcgctgg cagcgctttc tgcggcggtt gctgcacagc
    11941 tgccttgccg tgacgcgctg atgcaggagt acgacgataa gtggcatcag gacggtctgg
    12001 tgatggataa atggtttatc ctgcaagcca ccagcccggc ggcgaatgtg ctggagacgg
    12061 tgcgtggtct gttgcagcat cgctcattta ccatgagcaa cccgaaccgt attcgttcgt
    12121 tgattggtgc gtttgcgggc agcaacccgg cagcgttcca tgccgaagat ggcagcggtt
    12181 accagttcct ggtggaaatg cttaccgacc tcaacagccg taacccgcag gtagcttcac
    12241 gtctgattga accgctgatt cgcctgaaac gttatgatgc caaacgtcag gagaaaatgc
    12301 gcgcggcgct ggaacagttg aaagggctgg aaaatctctc tggcgatctt tacgagaaga
    12361 taactaaagc actggcttga tacttttgag ccaaaatgcc tgatgcgacg cttgacgcgt
    12421 cttatcaggc ctacaactcc gttgaaattg ctggttttgt aggctggata aggcgttcac
    12481 gccgcatccg gcagccgtgc ccaactgcct gatgcgatgc ttgacgtgtc ttatcaggcc
    12541 tacaagtccg ttgaaaatgc tggttttgtg cgccacatcc ggcatattaa ccatgacgcc
    12601 gaatcagttg ttcagaatgc ccacgccgca ttacccgttg caatacttcc gcttccagct
    12661 ccgctaatcg caccgaccct aatcgacgcg gacgggggat atccactgtc aaatccagac
    12721 taatttttcc ctcttcaatt aacaacaccc ggtcagccat tgctaccgct tcgctcacat
    12781 catgcgtcac caacagtacg gtaaagccgt gctgctgcca aagtgacaca atcaaatcct
    12841 gcatctcgag tcgcgttaag gcgtccagtg ccccgagcgg ttcatcaagc aacaataaac
    12901 ccggtcgatg aatcaacgct cttgccagcg ccacacgctg tttctgcccg ccagaaagtg
    12961 cggcaggcca ttccccggcg cgattctcca gtcctgccgc agccaacgcc cgacgcgcgg
    13021 catcgcgcca ctgaccttta aggcctaacc caacgttatc aatcaccgat ttccacggca
    13081 gcagacgcgc atcctgaaac atcattcgcg tatcatcctg aatttcagcc agcggtgtgg
    13141 tgcccgctaa cacatcgcct gcggttggcg tttccagacc ggccagcagg tgcagcaggg
    13201 tacttttacc accaccgctg cggcccacca ccgccacaaa ctgacctgcc ggaatatgta
    13261 aatccagttg gttcaggacg atattttccg cgtaatgttt gcttactgca ttgagcaaca
    13321 atggtgtgcc ctggttcaga cgagcagtat tcatatcgtg gcctccttca aatgataagc
    13381 cgggttccag cgcaaccaga ggcgctctaa cagctgcgcg ctgacgtcag caagtttgcc
    13441 gagcagggcg taaagaataa tagcgactac caccacgtct gtttgcaaaa actctcgcgc
    13501 attcatcgcc agataaccaa tgccggaatt ggcagaaatg gtttcggcaa caatcagcgt
    13561 cagccacatc aggcccaacg caaaacgcac gccgaccata attgagggca gggcatcggg
    13621 caggatcaca tggataaaca gcggtatgcc ggataatcca tagctacgcg ccatctccac
    13681 cagcccgcga tcgatattac ggatgccatg
//
LOCUS       DAJTRD010000094        13210 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTRD010000094 DAJTRD010000000
VERSION     DAJTRD010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13210
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.094"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(18..1001)
                     /gene="kdgT"
                     /locus_tag="OO315_003186"
     CDS             complement(18..1001)
                     /gene="kdgT"
                     /locus_tag="OO315_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR5891995.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(1261..1881)
                     /gene="sodA"
                     /locus_tag="OO315_003187"
     CDS             complement(1261..1881)
                     /gene="sodA"
                     /locus_tag="OO315_003187"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5891996.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2166..3200
                     /gene="rhaT"
                     /locus_tag="OO315_003188"
                     /pseudo
     CDS             2166..3200
                     /gene="rhaT"
                     /locus_tag="OO315_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3197..4063)
                     /gene="rhaR"
                     /locus_tag="OO315_003189"
     CDS             complement(3197..4063)
                     /gene="rhaR"
                     /locus_tag="OO315_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5891997.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(4119..4955)
                     /gene="rhaS"
                     /locus_tag="OO315_003190"
     CDS             complement(4119..4955)
                     /gene="rhaS"
                     /locus_tag="OO315_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5891998.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5243..6712
                     /gene="rhaB"
                     /locus_tag="OO315_003191"
     CDS             5243..6712
                     /gene="rhaB"
                     /locus_tag="OO315_003191"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5891999.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6709..7927
                     /gene="rhaA"
                     /locus_tag="OO315_003192"
                     /pseudo
     CDS             6709..7927
                     /gene="rhaA"
                     /locus_tag="OO315_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
     gene            8169..8993
                     /gene="rhaD"
                     /locus_tag="OO315_003193"
                     /pseudo
     CDS             8169..8993
                     /gene="rhaD"
                     /locus_tag="OO315_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            9003..9317
                     /gene="rhaM"
                     /locus_tag="OO315_003194"
     CDS             9003..9317
                     /gene="rhaM"
                     /locus_tag="OO315_003194"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5892000.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9618..10064
                     /locus_tag="OO315_003195"
     CDS             9618..10064
                     /locus_tag="OO315_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5892001.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            10075..11526
                     /locus_tag="OO315_003196"
     CDS             10075..11526
                     /locus_tag="OO315_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5892002.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11516..12586
                     /locus_tag="OO315_003197"
     CDS             11516..12586
                     /locus_tag="OO315_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5892003.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12586..>13210
                     /locus_tag="OO315_003198"
     CDS             12586..>13210
                     /locus_tag="OO315_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR5892004.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3147 a         3450 c         3481 g         3132 t
ORIGIN      
        1 ggattaagga agcaggttta tttcaccgta cctaaaattt cgattttcgc tgctctggct
       61 ttgactttac gtgaccagat agaggtaaga attggcacca gaatcgaagt cacaatgacc
      121 gccgtcgcta ccagcgaagt tgctgccgga gccatcggtt taaacgcagg caccatttct
      181 gcaatcagca caggtgtcgc taccgcggcc cctgcggaac tggaagcggc aattccggca
      241 gtgccatcgc caccgccaat caatttatcg gcgataatca acggaatacc ggtcacgata
      301 attactgcca cacccaacag gatccccagc aaaccagtct gggcaatcac agtcaaatca
      361 atggtattgc ccagcgcgaa ggcaaagaat ggaatcagcg tttgcaccgc tttgctgaaa
      421 aattctcgta attcagggtc aagattcccc agggcaaagc ccaccaggaa cggtaatact
      481 gcgccgacga aaacatgcgg ttcaaacgag gcaatcccgg cagtgcccag aataatcatc
      541 gtcatgagcg gcccggactc caacgacatc aacacaaatg ccccagcttc ttctttcgta
      601 ccgtactgct gcatgatgga agcgtaaagt ccgccgttgg tcatatccat cgccgccacc
      661 agcgccagcg ttgaaagtcc ggcaaagaat ccaacttcaa caccatgttc cggaataatg
      721 cgcgaggcaa ttgccgcaac cacccacgcg acggcaattt tagttaccac cagcgtaccg
      781 gatttacgca gtaccgttcc cgtcgcgctt aattttattg acgcccccat gcaaaaaaac
      841 cacaccgcca gaatgggcac cgtaccggta atcatcccgt tggtgaatga tccaaaatat
      901 ttccccgccc ccggcgagaa ggtgtggcac agtgcgccaa ggaatagcgg gacgagcatc
      961 atcccccccg ggattttctc aatcgagcgt tttatctgca tttccatcac ctgtcggcat
     1021 atttttaagg gctgatgcgt aattcctcaa tggagcatca gtgttgatgg aaacgaagat
     1081 agacattcat ttaatcaata aaagtgatca aaatcacatc aattgaaacg ctgttttatt
     1141 tttataggac cattgttttt attttggaat tgatcacaaa aaaaccaccg ccgttggcga
     1201 tggttcattt tttaagctga tatgcggcct atacgcctca ttgcagcagg cggcaaatga
     1261 ttattttttc gccgcaaaac gtgccgctgc ttcgtcccag ttcaccacgt tccagaactc
     1321 tttgatgtag tccggacggc ggttctggaa tttcaggtag taagcatgtt cccacacatc
     1381 caggcccaga atcgggaagc cggaagcgcc agaaatagct tcacccatca gcggagaatc
     1441 ctggttagca gtagaaacca ccgccagttt atcgcctttc agcaccagcc atgcccagcc
     1501 ggaaccaaag cgggaagctg ccgctttttc aaattctgct ttgaagttat caacggagcc
     1561 gaagtcacgt tcgatagccg ctttcaggtc accctgcagg gtggtgcctt ttttcagacc
     1621 tttccagaac aggctgtggt tagcgtgacc gccagcgttg ttgcgcagta cggttttctt
     1681 gtctgctggc agctggtcca gtttggtgat cagctcttca accggcaggt tggcaaattc
     1741 tggcaggctt tccagcgccg cgttggcgtt gtttacgtag gtctgatggt gtttggtgtg
     1801 gtggatttcc atggtctgct tatcgaagtg cggttccagg gcatcgtaag catacggcag
     1861 ggatggcagg gtatagctca tattcatctc cagtattgtc gggcggccga ttgttaatgc
     1921 cgcgtaagca gttggttcat tatagttaat taaatgatat tgaaaatgat tatcaatgcc
     1981 gtacttttcg taagggtatg gttttgcagg aaaatgcccg agatgtgaag caaatcaccc
     2041 acttaatgcc gtgattgcca gtaaatcgac aacggcggca acaggcgaaa ggttaatcga
     2101 cagcacgatt tttacactca tctcgtcgga gatgtgaagc gacgaaaaat gatgaggata
     2161 agaagatgag taacgcgatt acgatgggga tattttggca tttgatcggc gcggccagtg
     2221 cagcctgttt ttacgctccg ttcaaaaaag taaaaaaatg gtcatgggaa accatgtggt
     2281 cagtcggtgg gattgtttcg tggattattc tgccgtgggc catcagcgcc ctgttactac
     2341 cggatttctg ggcgtattac agctcgttta gtctctctac gctgctgcct gtttttctgt
     2401 tcggcgctat gtgggggatc ggtaatatca actatggcct gaccatgcgt tatctcggca
     2461 tgtcgatggg aattggcatc gccattggca ttacgttgat tgtcggtacg ctgatgacgc
     2521 caattatcaa cggcaatttc gatgtgctga ttaacaccga aggcggacgc atgacgttac
     2581 tcggcgttct ggtggcgctg attggcgtag ggattgtaac tcgagccggg cagttgaaag
     2641 agcgcaagat gggcattaaa gccgaagagt tcaatctgaa aaaagggctg gtgctggcgg
     2701 tgatgtgcgg cattttctct gccgggatgt cctttgcgat gaacgccgca aaaccgatgc
     2761 atgaagccgc tgccgcactt ggcgtcgatc cactgtatgt tgctctgcca agctatgttg
     2821 tcatcatggg cggcggcgcg atcattaacc tcggtttctg ttttattcgt ctggcaaaag
     2881 tgaaggattt gtcgctaaaa gccgacttct cgctggcaaa accgctgatc attcacaatg
     2941 tgttactctc ggcactgggc gggttgatgt ggtatctgca attctttttc tatgcctagg
     3001 gccacgcccg cattccggcg cagtatgact acatcagttg gatgctgcat atgagtttct
     3061 atgtattgtg cggcggtatc gtcgggctgg tgctgaaaga gtggaacaat acaggacgcc
     3121 gtccggtaac ggtgttgagc ctcggttgtg tggtgattat tgtcgccgct aacatcgtcg
     3181 gcatcggcat ggcgaattaa tctttctgcg aattgagatg acgccactgg ctgggcgtca
     3241 tcccggtttc ccgggtaaac accaccgaaa aatagttact atcttcaaag ccacattcgg
     3301 tcgaaatatc actgattaac aggcggctat gctggagaag atattgcgca tgacacactc
     3361 tgacctgccg cagatattga ttgatggtca ttccagtctg ctggcgaaat tgctgacgca
     3421 aaacgcgctc actgcacgat gcctcatcac aaaatttatc cagcgcaaag ggacttttca
     3481 ggctagccgc cagccgggta atcagcttat ccagcaacgt ttcgctggat gttggcggca
     3541 acgaatcact ggtgtaacga tggcgattca gcaacatcac caactgcccg aacagcaact
     3601 cagccatttc gttagcaaac ggcacatgct gactactttc atgctcaagc tgaccgataa
     3661 cctgccgcgc ctgcgccatc cccacgctac ctaagcgcca gtgtggttgc cctgcgctgg
     3721 cgctaaatcc cggaatcgct ccctgccagt caagattcag cttcagccgc tccgggcaat
     3781 aaataatatt ctgcaaaacc agatcgttaa cggaagcgta ggagtgttta tcgtcagcat
     3841 gaatataaaa gagatcgcca cgggtaatgc gataaggacg atcgttgagt acatgcagac
     3901 cattaccgcg ccagacaatc accagctcac aaaaatcatg tgtatgttcg gcaaagacat
     3961 cttgcggata acggtcagcc acagcgactg cctgctggtc gctggcaaaa aaatcatctt
     4021 tgagaagttt taactgatgc gccaccgtgg ctacctcggc cagagaacga agttgattat
     4081 tcgcaatatg gcgtacaaat acgttgagaa gattcgcgtt attgcagaaa gccatcccgt
     4141 ccctggcgaa tatcacgcgg tgaccagtta aactctcggc gaaaaagcgt cgaaaagtgg
     4201 ttactgtcgc tgaatccaca gcgataggcg atgtcagtga cgctggcctc gctgtggcgt
     4261 agtagatgtc gggctttcat cagtcgcagg cggttcaggt atcgctgagg cgtcagtccc
     4321 gtttgctgct taagctgccg atgtagcgta cgcagtgaaa gagaaaattg atccgccacg
     4381 gcatcccaat tcacctcatc ggcaaaatgg tcctccagcc aggccagaag caagttgaga
     4441 cgtgatgcgc tgttttccag gttctcctgc aaactgcttt tacgcagcaa gagcagtaat
     4501 tgcataaaca agatctcgcg actggcggtc gagggtaaat cattttcccc ttcctgctgt
     4561 tccatctgtg caaccagctg tcgcacctgc tgcaatacgc tgtggttaac gcgccagtga
     4621 gacggatact gcccatccag ctcttgtggc agcaactgat tcagcccggc gagaaactga
     4681 aatcgatccg gcgagcgata cagcacattg gtcagacaca gattatcggt atgttcatac
     4741 agatgccgat catgatcgcg tacgaaacag accgtgccac cggtgatggt atagggctgc
     4801 ccattaaaca catgaatacc cgtgccatgt tcgacaatca caatttcatg aaaatcatga
     4861 tgatgttcag gaaaatccgc ctgcgggagc cggggttcta tcgccacgga cgcgttacaa
     4921 gacggaaaaa aatccacact atgtaatacg gtcatactgg cctcctgatg tcgtcaacac
     4981 ggcgaaatag taatcacgag gtcaggttct taccttaaat tttcgacgga aaaccacgta
     5041 aaaaacgtcg atttttcaag atacagcgtg aattttcagg aaatgcggtg agcatcacat
     5101 caccacaatt cagcaaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc
     5161 ccattttcct gtcagtaacg agaaggtcgc gaattcaggc gctttttaga ctggtcgtaa
     5221 tgaaattcag caggatcaca ttatgacctt tcgcaattgt gtcgccgtcg atctcggcgc
     5281 atccagtggg cgcgtgatgc tggcgcgtta cgagcgtgaa tgccgcagcc tgacgctgcg
     5341 cgaaatccat cgttttaaca atgggctgca tagccaaaac ggttatgtca cctgggatgt
     5401 ggatagcctg gaaagtgcca ttcgccttgg attaaacaag gtgtgcgagg aagggattcg
     5461 tatcgatagc attgggattg atacctgggg cgtggacttt gtgctgctcg accaacaggg
     5521 tcagcgtgtg ggcctgcccg ttgcttatcg cgatagccgc accaatggcc tgatgccgca
     5581 ggcacaacag caactcggca aacgcgatat ttatcagcgt agcggcatcc agtttctacc
     5641 cttcaatacg ctttatcagt tgcgtgcgct gacggagcaa caacctgaac ttattccaca
     5701 cattgctcac gctctgctga tgccggatta cttcagttat cgcctgaccg gcaagatgaa
     5761 ctgggaatat accaatgcca ccaccacaca actggtcaat atcaatagcg acgactggga
     5821 cgagtcgcta ctggcgtgga gcggggccaa caaagcctgg tttggtcgcc cgacgcatcc
     5881 gggtaatgtc attgggcact ggatttgccc gcagggtaat gagattccgg tggtcgccgt
     5941 tgccagccat gataccgcca gcgcggttat cgcctcgccg ttaaacggtt cacgcgccgc
     6001 ttatctctct tctggcacct ggtcattgat gggcttcgaa agccagacgc catttaccaa
     6061 tgacacggcg ctggcagcca acatcaccaa tgaaggcggg gcggaaggtc gctatcgggt
     6121 gctgaaaaat attatgggct tatggctgct tcagcgagtg cttcaggaac ggcaaatcaa
     6181 cgatctcccg gcgcttatcg ccgcgacaca ggcacttccg gcctgccgct tcatcatcaa
     6241 tcccaatgac gatcgcttta ttaatcctga cgagatgtgc agcgaaattc aggctgcgtg
     6301 tcgggaaacg gcgcaaccga tcccgggaag tgatgctgaa ctggcgcgct gtattttcga
     6361 cagtctggcg ctgctgtatg ccgatgtgtt gcatgagctg gcgcagctgc gcggtgaaga
     6421 tttctcgcaa ctgcatattg tcggcggcgg ctgccagaac acgctgctca accagctatg
     6481 cgccgatgcc tgcggtattc gggtgatcgc cgggcctgtt gaagcctcaa cgctcggcaa
     6541 tatcggcatc gagttaatga cgctggatga actcaacaat gtggatgatt tccgtcaggt
     6601 cgtcagcacc accgcgaatc tgaccacctt tacccctaat cctgacagtg aaattgccca
     6661 ctatgtggcg cagattcact ctacacgaca gacaaaggag ctttgcgcat gaccactcaa
     6721 ctggaacagg cctgggagct ggcgaaacag cgtttcgcgg cggtggggat tgatgtcgag
     6781 gaggcgctgc gccaacttga tcgtttaccc gtttcaatgc actgctggca gggcgatgat
     6841 gtttccggtt ttgaaaaccc ggaaggttcg ctgaccgggg ggattcaggc cacaggcaat
     6901 tatccgggca aagcgcgtaa tgccagtgag ctacgtgccg atctggaaca ggctatgcgg
     6961 ctgattccgg ggccgaaacg gcttaattta catgccatct atctggaatc agatacgcca
     7021 gtctcgcgcg accagatcaa accagagcac ttcaaaaact gggttgaatg ggcgaaagcc
     7081 aatcagctcg gtctggattt taacccctcc tgcttttcgc atccgctaag cgccgatggc
     7141 tttacgctct cgcatcccga cgacagcatt cgccagttct ggattgatca ctgcaaagcc
     7201 agccgtcgcg tttcggccta ttttggcgag caactcggca caccatcggt gatgaacatc
     7261 tggatcccgg atggtatgaa agatatcacc gttgaccgtc tcgccccgcg tcagcgtctg
     7321 ctggcagcac tggatgaggt gatcagcgag aagctaaacc ctgcgcacca tatcgacgcc
     7381 gttgagagca aattgtttgg cattggcgca gagagctaca cggttggctc caatgagttt
     7441 tacatggggt atgccaccag ccgccagact gcgctgtgcc tggacgccgg gcacttccac
     7501 ccgactgaag taatttccga caagatttcc gccgccatgc tgtatgtacc gcagttgctg
     7561 ctgcacgtca gccgtccggt tcgctgggac agcgatcacg tagtgctgct ggatgatgaa
     7621 acccaggcaa ttgccagtga gattgtgcgt cacgatctgt ttgaccgggt gcatatcggc
     7681 cttgacttct tcgatgcctc tatcaaccgc attgccgcgt gcgttgctgg aacctaccgc
     7741 tgagctgcgc aagctggaag cggcgggcga ttacactgcg cgtctggcac tgctggaaga
     7801 gcagaaatcg ttgccgcggc aggcggtctg ggaaatgtat tgccaacatc acgatacgcc
     7861 agcaggtagc gaatggctgg agagcgtgcg ggcttatgag aaagcgattt tgagccagcg
     7921 tgggtaaaca ctgccggatg cggcacgagc gccttatccg gcctacgggt cgaatgtcca
     7981 tgcgggctgt caccctcacc ccggccctct ccctgaaagg gcgagggaga aaaacatagc
     8041 ctcgtgcggc aacggagctg aacaccgctc cggacagtcc cctcgcccct acggggagag
     8101 ggttagggtg aggggaacaa gccgcaccat gtctaaacaa ggcaacctaa tttacaggaa
     8161 cacagaacat gcaaaacatt acccagtcct ggtttgtcca gggaatgatc aaagccacca
     8221 ccgatgccta gctgaaaggc tgggacgagc gcaacggcgg caacctgacg ctgcgcctgg
     8281 atgacgccga tatcgcgctt tatcacgaca acttccaccc acaaccgcgc tatattccgc
     8341 tcagccaacc catgccttta ctggcaaata cgccgtttat tgtcaccggc tccggcaaat
     8401 tcttccgtaa cgtccagctc gatcctgcag ctaacttagg cgtcgtaaaa gtcgacagcg
     8461 atggcgcggg ctaccatatt ctctgggggt taaccaacga agccgttcca acttctgaac
     8521 ttccggctca ctttctctcc cactgcgagc gcattaaagc caccaacggt aaagatcggg
     8581 tgatcatgca ctgccacgcc accaacctga tcgccctcac ctatgtactg gaaaacgaca
     8641 ccgcggtctt tactcgccaa ctgtgggaag gcagcaccga atgtctggta gtattcccgg
     8701 atggcgttgg cattctgccg tggatggtgc ccggcacgga cgaaatcggc caggcgaccg
     8761 cacaagagat gcaaaaacat tcgctggtgt tgtggccctt ccacggtgtg ttcggttccg
     8821 gaccgacgct ggatgaagcc tttggtttaa tcgacaccgc agaaaaatca gcacaagtat
     8881 tagtgaaggt ttattcgatg ggcggcatga aacagaccat cagccgtgaa gagttgatag
     8941 cgctcggcaa gcgcttcggc gttacaccac tagccagtgc gctggcgctg taaggagcaa
     9001 acatgatccg caaagccttt gtcatgcagg taaaccccga cgcccacgaa gagtatcagc
     9061 gtcggcataa tcccatctgg ccggaactgg aagcagtgtt gaaatctcac ggtgtgcata
     9121 actacgccat ttacctcgac aaagcgcgta atctgctgtt tgccatggta gagattgaat
     9181 ctgaagaacg gtggaatgcg gtagccagta ctgatgtttg ccaacgttgg tggaaatata
     9241 tgaccgatgt tatgcccgct aacccagata acagcccggt gagtagcgag ctgcaagaag
     9301 tgttttacct gccgtaattg cccaatgtgc cggagatgcc tccggcacaa tattttaaat
     9361 ttcgatttga aaaattaaac atattacatc tcccggtaag ttatatttcc ctgatacatt
     9421 gtgagtaaat cacaaaaata atgaataacc cattaatgat tcatgtggtt tatttaaata
     9481 accattatgt gcattactcc gcaaatctga cctttcactc tgtctcaaat tgtcattgtt
     9541 taccgacata tcgcctctca tcacttaatc gctccaccat tacaattgat taaaatatat
     9601 tttatggagt ccgattaatg gcagctctta ctgcaagctg tattgacctg aatattcagg
     9661 gcaatggcgc ttattccgtt ctgaagcagt tggcgacaat agcgttacaa aacggtttta
     9721 tcaccgactc acaccagttc ctgcaaaccc tgctgctgcg cgaaaaaatg cactctactg
     9781 gatttggttc cggtgtcgca gtgccgcacg gtaaaagcgc ctgcgttaaa caaccgttcg
     9841 tattattcgc ccgcaaagcg caggctattg actggaaagc cagcgatggc gaagacgtca
     9901 attgctggat ctgcctcggc gtgccgcaaa gcggcgaaga ggatcaggtc aaaatcatcg
     9961 gcacactgtg tcgcaaaatt attcaccagg aatttattca tcaactgcaa cagggcgata
    10021 ccgaccaggt gctggccttg ttaaatcaaa ccctcagctc ataaggaagt ggcgatggag
    10081 tcatccttac gtattgtcgc gatcaccaac tgccccgccg ggatcgctca cacctacatg
    10141 gtggcggaag ccctggaaca gaaagcccgt tctctcggcc ataccataaa agtggaaact
    10201 caagggtcca gtggcgttga aaaccgctta tccagcgaag agattgccgc tgccgattac
    10261 gtcattcttg ctaccgggcg tggcctgagc ggtgatgatc gcgcgcggtt tgccgggaag
    10321 aaagtttatg agattgccat ctccctggcg ttgaaaaata tcgaccagat tttcagcgaa
    10381 ttaccgacaa actcgcagct ttttgccgca gatagcggcg tgaagcttgg caaacaggaa
    10441 gtgcaaagcg gcagcgtgat gagccacctg atggcgggcg tctctgcggc gctgccgttt
    10501 gtcatcggcg gtggtatcct ggtggcgctt gccaacatgc tggtgcagtt cggtttgccg
    10561 tataccgata tgtcgaaagg cgcgccgtcg tttacctggg tagttgaatc catcggttac
    10621 cttggcttca cctttatgat ccccatcatg ggcgcttata ttgcctcgtc gattgccgat
    10681 aaaccggctt tcgctccggc gtttctggtt tgctatctgg cgaacgacaa agcgctgctc
    10741 ggcacccagt cgggtgcggg attcctcggc gcggtggtgc tggggctggc gattggctat
    10801 ttcgtcttct ggttccgcaa agtgcgcctt ggcaaagcat tacaaccgct gctcggttca
    10861 atgctgatcc cgttcgttac tctgctggtt ttcggcgtgc tcacttacta cgttatcggg
    10921 ccggtgatgt ccgacctcat gggcgggctg ctccacttcc tgaataccat tcctccatca
    10981 atgaagtttg cggcagcgtt tctggtcggt gcgatgctgg cgttcgatat gggcggcccc
    11041 atcaataaaa ccgcctggtt cttctgcttc tcactgctgg aaaaacacat ttatgactgg
    11101 tacgccatcg tcggcgttgt cgcgttgatg ccgcccgttg cggcgggtct tgcgacgttc
    11161 attgcgccca aactgtttac ccgacaggaa aaagaggctg ccagtagtgc cattgtggtc
    11221 ggtgccacag tcgcaaccga accggctata ccttacgctc tggcagctcc gttgccgatg
    11281 attaccgcca atacgctggc gggcggcatt accggcgtgc tggtgatcgc tttcggaatc
    11341 aaacgtctgg cacctggttt gggtatcttt gacccgctga tcggcctgat gtcgccggta
    11401 ggttcgtttt atctggtgct ggcgattggc ctcgcgctga acatttcatt cattatcgtc
    11461 ctgaaaggat tgtggttacg ccgcaaagcg aaagccgcgc aacaggagct tgtccatgaa
    11521 cattgagtta ctgcaacagt tgtgcgaagc cagcgccgtt agcggcgatg aacaggaagt
    11581 tcgcgacatc ctgataaaca cgctggaacc ttgcgttaat gaaatcacct ttgatggtct
    11641 gggcagcttt gttgcccgta agggaaataa aggtccaaaa gttgccgttg tcgggcatat
    11701 ggatgaagtc ggctttatgg tcacccacat cgacgagagc ggttttctgc gtttcaccac
    11761 cattggtggc tggtggaatc agtcgatgct caaccatcgg gtaaccatac gcacacacaa
    11821 gggagtgaaa atccctggtg tgattggttt cgtcgcgcct catgcgttaa cggaaaagca
    11881 aaggcaacag ccgctgtcat ttgatgagat gttcattgat attggcgcaa acagtcgcga
    11941 tgaggtggaa aaacgcggtg tggcgatggg cgattttatt agcccggaag ccaattttgc
    12001 ctgctggggc gaagataaag tggtcggcaa ggcgctggat aaccgcatcg gctgcgcaat
    12061 gatggctgaa ctattgcaga cggtgaataa tcccgaaatt acgctgtatg gcgtcggcag
    12121 cgtggaagaa gaagttgggc tacgcggggc acaaacctcg gcggaacaca ttaaaccgga
    12181 tgtagtgatc gtgctggata ccgctgtcgc aggcgatgtt ccgggcattg ataacattaa
    12241 atacccgctg aaactgggcc aggggccggg gctgatgctg tttgacaagc gctacttccc
    12301 caaccagaaa ctggtagcag cgttaaaaaa ctgtgccgca cataacgatt taccgctgca
    12361 attttccacc atgaaaaccg gagcgacgga tggcgggcgc tacaacgtga tgggcggcgg
    12421 gcgtccggtt gttgcgctgt gcctgccaac ccgctatctg cacgctaaca gcggcatgat
    12481 ttcaaaagcc gattacgatg ctctgctcac gttgatacgg ggttttctga cgaccttaac
    12541 cgcggagaaa gtcaacgcgt ttagccagtt ccgtcaggtg gattaatgct aaacgaacgc
    12601 cagttaaaga ttgtcgatct gctggagcaa cagccgcgca cgcctggtga gctggcgcaa
    12661 cagactggcg tttcaggcag gaccatcctg cgtgatattg actatctcaa ctttaccctt
    12721 aacggcaaag cccgcatttt cgccagtggc agtgcgggct atcagctgga aatcttcgag
    12781 cgccgcagct tttttcagtt gctgcaaaag cacgataacg acgatcggct gctggcgctg
    12841 ttattactga atactttcac tccccgtgcg caactcgcct cggcgcttaa tttgccagaa
    12901 acgtgggtag cagagcgtct gccccagtta aaacagcgtt atgaacgcac ttgttgcctg
    12961 gccagccgcc ctggtttggg ccatttcatt gatgagacag aagagaaact cgttatcttg
    13021 ctggcgaact tgctgcgcaa agatccgttt ttaattccgc tggcgggcat aacacgagac
    13081 aaccttcagc atttatccac ggcctgcgac aaccaacacc gctggccgct catacagggt
    13141 gattatctct ccagcctgat tctggcgatt tacgccctgc gtaatcaact gaccgatgag
    13201 tggccgcaat
//
LOCUS       DAJTRD010000095        13144 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTRD010000095 DAJTRD010000000
VERSION     DAJTRD010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.095"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..893)
                     /locus_tag="OO315_003199"
     CDS             complement(<1..893)
                     /locus_tag="OO315_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5892005.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPG"
     gene            1134..1934
                     /locus_tag="OO315_003200"
     CDS             1134..1934
                     /locus_tag="OO315_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5892006.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            2012..2782
                     /locus_tag="OO315_003201"
     CDS             2012..2782
                     /locus_tag="OO315_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5892007.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2830..4176)
                     /gene="mltD"
                     /locus_tag="OO315_003202"
     CDS             complement(2830..4176)
                     /gene="mltD"
                     /locus_tag="OO315_003202"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5892008.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4248..5003)
                     /gene="gloB"
                     /locus_tag="OO315_003203"
     CDS             complement(4248..5003)
                     /gene="gloB"
                     /locus_tag="OO315_003203"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5892009.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5037..5759
                     /locus_tag="OO315_003204"
     CDS             5037..5759
                     /locus_tag="OO315_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5892010.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5756..6223)
                     /gene="rnhA"
                     /locus_tag="OO315_003205"
     CDS             complement(5756..6223)
                     /gene="rnhA"
                     /locus_tag="OO315_003205"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5892011.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6288..7019
                     /gene="dnaQ"
                     /locus_tag="OO315_003206"
     CDS             6288..7019
                     /gene="dnaQ"
                     /locus_tag="OO315_003206"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5892012.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7152..7228
                     /locus_tag="OO315_003207"
     tRNA            7152..7228
                     /locus_tag="OO315_003207"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7186..7188,aa:Asp,seq:gtc)
     gene            complement(7359..8405)
                     /locus_tag="OO315_003208"
     CDS             complement(7359..8405)
                     /locus_tag="OO315_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5892013.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8419..8978)
                     /gene="tssG"
                     /locus_tag="OO315_003209"
                     /pseudo
     CDS             complement(8419..8978)
                     /gene="tssG"
                     /locus_tag="OO315_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8975..10858)
                     /gene="tssF"
                     /locus_tag="OO315_003210"
     CDS             complement(8975..10858)
                     /gene="tssF"
                     /locus_tag="OO315_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5892014.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10874..11368)
                     /gene="tssE"
                     /locus_tag="OO315_003211"
     CDS             complement(10874..11368)
                     /gene="tssE"
                     /locus_tag="OO315_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5892015.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11365..12195)
                     /locus_tag="OO315_003212"
                     /pseudo
     CDS             complement(11365..12195)
                     /locus_tag="OO315_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12182..13096)
                     /gene="tagK"
                     /locus_tag="OO315_003213"
                     /pseudo
     CDS             complement(12182..13096)
                     /gene="tagK"
                     /locus_tag="OO315_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3020 a         3409 c         3491 g         3224 t
ORIGIN      
        1 ccgggagctg tttttacatg ctcgctaagg aaatcgataa aagcccggat gcgcgtactt
       61 accgcacggt cgctgtaata gacggcactg aatggcattt ccactggcaa cactttatct
      121 gccattaact ccaccaattc tccgcgagcg atttctttgt cgatcatgta gtcggacaaa
      181 cacgcaatcc cgttgccact caggcaaagc tgtttcagtg tttccccact attggatgac
      241 aaaccgtact tcacctcatg taattgtcca tcactacagg ctatcggcca ggtattgagg
      301 gaagcgggtt cagtgaatcc caggcaaaca tgttgcttta aatcgtcgat cgtttctggc
      361 ttcccgtagc gggaaatata atcgggggag gcgataattt ttcgataact gttaaataac
      421 ggcctggcac gtaagctgga gtccgttaac gtaccagcgc gtatcgcgac atccactttt
      481 ctttcgatca aattaataat cgtttcggag gagactagcg ataaagtgac ttccggatag
      541 cgttcacgga aaggcttaat taacggcatc agaaagtgca gcaccactgg agttgcggca
      601 tcgatccgta acagtccacg cggtgtatta cgcgtctcca taatttctga ttctgccgct
      661 gccatctcct gcaaaattga ctgtacgcga cgaaaataac gctcgccttc ttccgtcagg
      721 ctaagttgcc gcgtggtccg attaagcagg ctaacgccaa gtttcatttc cagctttttc
      781 accgcccggc ttaccgctga gtttgcttgc cctaattgtt ccgctgcccg gctaaagctg
      841 ccgctttcga cgaccgaaac aaaaatggcg agttcttccg acgtggcttt catttttgct
      901 cctgttgcaa aatagatgag atattttgaa tttatttgtc attaaaccat caggatgcgt
      961 gatatgtcat ccgatttaat gttctcaata atgagcaaat ttctgacagg tgtaagcact
     1021 tgcttacata acaatataca attgctcgtt gaaagagtga gctaaaatcc ctataacagt
     1081 agaaccctcc cgagtgcgga agggttgacg taatagaggt ttcaaagtca aaagtgcgaa
     1141 aaaacaccta tgccatgcgc tatgttgccg gacaacctgc ggaaaggatc ttaccgccgg
     1201 ggtcttttgc gagcatcggc caggcattac cacctggaga accgttaagt accgaagagc
     1261 gtattcggat cctggtgtgg aacatataca aacagcaacg cgctgaatgg ttgtcggtat
     1321 taaagaacta cggcaaagat gcacatctgg tgttattgca ggaagcgcag acaacgccag
     1381 agttagtaca gtttgcgacc gctaactatc ttgccgccga tcaggtgccc gctttcgtgc
     1441 tgccacaaca tccttccggt gtaatgaccc tttcggcggc gcatccagtg tattgctgcc
     1501 cgttacgtga acgagaaccc attttgcgtc tggcgaagtc ggcactggtg acggtctatc
     1561 cattacctga cacccgcctg ttgatggtgg ttaatataca cgccgtcaac ttcagtctgg
     1621 gcgtggatgt ctatagtaag cagttacttc ctattggtga tcagatagct caccacagcg
     1681 gcccggtcat tatggcggga gatttcaatg cctggagccg tagaaggatg aacgcgttat
     1741 atcgctttgc gcgggaaatg tcgctgcgcc aggtgcgttt taccgatgat cagcgccgtc
     1801 gggcgtttgg ccgcccgctc gattttgttt tctaccgtgg tctgaacgtc agcgaagctt
     1861 ctgtattggt tacgcgcgct tccgatcaca atccgctact cgttgaattc agtcccggca
     1921 agcctgataa ataaggtatg tcaggtctgc cacagggcag accaacgttt ggcgctgtcc
     1981 ttttatactc aaacaacaaa aggacagcgc aatgacaaca caatcccacc atgaccacgt
     2041 agaaaagcag tttagctctc aagcctgcga atatctgacc agtaccgtac atgcatccgg
     2101 acgagattta cagcgcctgg cggtgcgtct ggctgattat cctggtgcaa gtgcacttga
     2161 tatgggctgc ggagcagggc atgccagctt tgttgctgcg caaaacgtga gcgcggtggt
     2221 ggcgtatgac ttatctgccc aaatgctgga tgtcgtggca caagctgccg aagcccggca
     2281 actgaaaaat atcaccaccc gccagggata tgccgaaagt ctgccatttg ccgataacgc
     2341 atttgatatt gttatcagcc gttattctgc ccatcactgg catgatgttg gtgcagcact
     2401 gcgtgaagtg aaccggatat tgaaacctgg cggtaggatg attgtgatgg acgtaatgtc
     2461 tccgggtcat ccagtgcgcg acatctggtt acagacggta gaagcattac gcgatacctc
     2521 tcacgtacga aactacgcca gcggtgagtg gttgaggtta atcaatgaag ccaatctgat
     2581 agttgataat ttaattacag ataagttacc gctggaattt tgttcatggg tcgcgagaat
     2641 gcgtacgcca gaagcgttag tagacgctat tcgcatttac caacagagcg catcgacaga
     2701 ggtgagaacg tattttgcct tgcagaatga cggctctttc accagtgata tcatcatggt
     2761 agaagcacat aaagcggcat aaataaaaaa ggcaccgggg gaatcggtac ctttttatta
     2821 tctggtttgt caggaatctg gcatgttgtt gtttttcaca aacaacgtca gcttgtcgcc
     2881 tggttgcaga ttcgcagtgt cgctgttcca gcgcatcaca tctttgatgt tcacgccgtg
     2941 gcgtttagca atgcttgaaa gcgaatcgcc tttgcgcaca cgatacgtaa tgctatcgct
     3001 gttgtttgcc agtcgctgtg caccaatcgt caaactttgg cctggcttca gcttagatcc
     3061 gcgcagtttg ttccactgct gcaaatcttt ggtgcttacg ccgagacgtg aagcgatgct
     3121 tgaaagcgtg tcgccagagc gtacggtgta aacacggctg ttaagcggcg tattgttggc
     3181 aaccagcgtc gactgtacag cagcaatttc gcctgaagcg agagattcac gcagttgatc
     3241 tgcatgcttc tttggcacca tcacgtactg cggcccactt gcgcccagcg tggagccttt
     3301 cacgccagcg ttgaatgtct tcagcttgct gacggaaatc cccgccatat ctgcaacctt
     3361 cgccatttca accgggctac tcaggtgcac acgcgccaga gcacggcttt catcggtcgt
     3421 tggcagacgt acgccataac gcttgctgtt tttgagaata tcactcaatg ccagcatttt
     3481 aggcacgtac tgcttcgttt cctgcggcaa cggtaacgac cagaagtccg tggatttccc
     3541 acgcgctttg ttcgttttaa ttgccttcat gacccgacct tcgccgctgt tataagccgc
     3601 tacggtcaga agccagtcgt cgtcaaacat tttgttcaga cgctgcatca tattcagcgc
     3661 ggcagttgtt gaagcaacaa catcgcgacg cgcgtcataa ttgcgggtct gtttcaaacc
     3721 ataattgcgc cccgtgctcg gaatgatctg ccagatgcct gcggcattgg cgccagacgt
     3781 tgcgtgagga tcaaaagcgc tctccactat gggtagtagt accagttcca taggcatgtt
     3841 acgtttttta acttgccctg ctatccagta catatacggc tctgcccgta aagttacatc
     3901 gtggagatag ctcttattgc gtaaatattt ctgtttctgt tcgcgaatcc ggtcattttc
     3961 cggaattccc atctttagct cgtcgccaat gaaagcccac aagtcaccat ctggcgcgat
     4021 agacgtccca tcgtccatcc atcgtgcctg acttgtaaac tttgctgctt ccccttgacc
     4081 agctgcagaa aggctctgtg cgtgctgttg aacgttgccg gtactctggc aacccacgag
     4141 caggacagag gcgagtaata tcgcttttgc cttcatgtgt gtgtcaatag ttgcttaaaa
     4201 gacgaccgat cataacggcg aacggagccg atgacaagaa agttttatca gaacctatct
     4261 ttctttgacc ttaaccatgc aaaacgctct tcaggttgtt gcaataatgt ttcttcatta
     4321 attccattaa ttaaatcaat atcttccgtt cttaaaaaaa cattaatttg ccgctcattt
     4381 ttcagaatta cgggtagtgt tatttgattt tttgcccgta actccttaac tttacgataa
     4441 taatcattta tggacaaatc gtgcggaaaa atactcaaag caaacttcat atttgataac
     4501 gtatattcat gagcacaaca taccaatgta tcgtcaggta acgcacttag ctttttaagt
     4561 gattgataca tttgtaaagc tgtcccttca aacaaccgac cacacccacc agaaaacagc
     4621 gtgtcgccgc aaaatagata aggtttactg aagtaacaga tatgtcctaa agtgtgaccc
     4681 ggtgtggtaa ttacactaaa ttcatgccct aaaacgaagg cagtttcgcc atctttgact
     4741 acctgtgttg ttcccttatc ttgtgtctct tgtggaccat acactacaat ttgcggaaac
     4801 ttttccacca gttcttttac gccgccaacg tgatcgtggt gatggtgggt tagaaatatg
     4861 gcctccggtt gccagttatt ggcggtaatg gcgtttaata ctggctctgc gtctccggga
     4921 tcgacaatca ggcagcgacc tgcctcatca ttcaaaaccc agatgtaatt gtcatcaaag
     4981 gcgggaatac tgttaagatt catagattac ctctcagtgt aaaacggaag gttgtgatga
     5041 aaccggcaag agtccctcaa actgtcgtgg ctcctgattg ctggggcgat ttgccctggg
     5101 gagagcttta tcgcaaggcg ctggagcgcc agctcaaccc gtggttcact aaaatgtatg
     5161 gttttcatct gcttaagatt ggcaatttaa gcgcagaaat caattgcgaa gcgtgcgcgg
     5221 tttctcatca agtgaatgtt tctgcgcaag gaatgcccgt ccaggtgcag gcggatccac
     5281 ttcatcttcc ttttgccgat aaatccgttg atatttgtct actggcacat accttgccgt
     5341 ggtgcaccga tccgcatcgt ttattacgtg aagccgatcg ggtactgatt gatgatggct
     5401 ggctggtgat tagtggcttc aaccccatca gtttaatggg attacgcaaa cttgtgccgg
     5461 tattgcgcaa aacctcgccc tataatagcc ggatgtttac tctgatgcgg caactggact
     5521 ggctctcttt gttgaatttt gaagtgctgc acgccagccg tttccacgtt ctcccgtgga
     5581 acaaacacgg aggaaaacta ttgaatgcgc atattcctgc gcttggttgc ttacaactta
     5641 ttgttgcccg gaaacggact attcctttaa cgctaaatcc gatgaaacag agtaaaaaca
     5701 agccacgaat tcgccaggcg gttggagcca cccggcaatg tcgtaaacca caggcttaaa
     5761 cttcgacttg gtagcccgta tcttccagtg tgggattcat cgccgcggca cgagccagtt
     5821 catcacagcg ttcgttttcc gggtgtccgg catggccttt aacccattcc catttgattt
     5881 gatgctgccc caatgcagca tcaagacgtt gccagagatc gacatttttt actggttttt
     5941 tgtctgctgt tttccagcca cgttttttcc agttatggat ccactgggtg ataccctggc
     6001 ggacatactg gctgtcggta ctcaaaatga cttcgcaatg ttcttttaac gcctccagcg
     6061 cgacaatagc ggccatcaac tccatacggt tgttggtggt gcgggtgtag cctgcgctaa
     6121 aggttttctc gcgtccgcga tagcgtaaaa tagcgccgta acccccaggt ccaggattac
     6181 ccagacacga accatcggtg aaaatttcta cctgtttaag catctctggt agacttcctg
     6241 taattgaatc gaactgtaaa acgacaagtc tgacataaat gaccgctatg agcactgcaa
     6301 ttacacgcca gatcgttctc gataccgaaa ccaccggtat gaaccagatt ggtgcacact
     6361 atgaaggcca caagatcatt gagattggtg ctgttgaagt agtgaaccgt cgcctgacgg
     6421 gcaataactt ccatgtttat ctcaaacccg atcggctggt ggatccggaa gcctttggcg
     6481 tacatggtat tgctgatgaa tttttgctcg ataagcccac gtttgccgaa gtagccgatg
     6541 agtttatgga ctatatccgc ggcgcggagt tggtgatcca taacgcagcg ttcgatatag
     6601 gctttatgga ctacgagttt tcgttgctta agcgcgatat tccgaagacc aataccttct
     6661 gtaaggtcac cgatagcctt gcggtggcga ggaaaatgtt tcccggtaaa cgcaacagcc
     6721 tcgatgcgct atgtgcccgc tacgaaatag ataacagtaa acgaacgctg cacggggcat
     6781 tactcgatgc ccagatcctt gcggaagttt atctggcgat gaccggtggt caaacgtcga
     6841 tggcttttgc gatggaagga gagacacagc agcaacaagg tgaagcaaca attcagcgca
     6901 ttgtacgtca ggcaagtaag ttacgcgttg tttttgcgac agatgaagag ctggcagcac
     6961 atgaagcccg tctcgatctg gtgcagaaaa aaggcggaag ctgcctctgg cgggcataaa
     7021 tacatgtaaa aggcgctaaa aatagcgact tgggcgattt ttgcagcaaa cgattcaaaa
     7081 gatgagaaaa atcgttgacg aaggtcgagg caatccgtaa tattcgcctc gttcccaacg
     7141 gaacacaacg cggagcggta gttcagtcgg ttagaatacc tgcctgtcac gcagggggtc
     7201 gcgggttcga gtcccgtccg ttccgccaat attcagaaag cccgaatcag taatgattcg
     7261 ggctttcgtc gttttggtga agtgagaatt tttcactccg aagtaaaacg gatatgattt
     7321 aaaaaatggc cagacattat aaattctggc cacttctgtt actccagcgc atcccatttc
     7381 tccagcagtt ctactggaat ttcatttatt acctccgaga cgcgttttcc gaccaggcgt
     7441 tctgcctggc gcagcaaagg aatggtcggg ctgctcggtt cgctgtgctc aaaccagcga
     7501 cggatgacac gcaggcgttc cagcgcgtcg tttcggtcgc ggatctgccg gggttctgcg
     7561 ccggtcatcg gtgatatttc ctccgcgata tgcaccggta gcgcaatctg ctctgcctgc
     7621 atctgttcag tatgaacgat ggtggcgttt tccggctgct cagcctgtgg ttgtggcagg
     7681 atctccggcg tggtgctctg cactgctccc ggtaacaggg ccagcagttg cgtcagacgg
     7741 gagaagtccg gtgcctggtc attcagcgtt tcacgcgcat ggcgttgcag ccgctcagtg
     7801 gtctctgctg cctggcgaaa ggcatccagc ggcagtgtgc cgcgcgcttc caggtctgcc
     7861 agttgctggc gtaccgattc tggtgccagc gcatccgccg ggcgtaatgc agatagcgaa
     7921 cgctccacat cccgcacctg aaggcgcatg gcagcattat ttgacagtgt aatgccacga
     7981 atatccgcca ttacaccttc gtggtccagc aacgccgcca gcgcgttact gcgtgccacc
     8041 gcagcatctt cggcggtgat atcttcagtc gcgagcagtt gaggatggat ggcgtccggg
     8101 tacgtggagc acaacgtttc cagcagcgtg agcgcctccg acagtcccct ggcaccggcc
     8161 tgctggatac ggcagcgcag caggacaacc agaatgcgga tatctttgct gcgcatcagc
     8221 agacgatggg cgtcccgctc aagctccgcc cagttaatgt tttccggcgt actgacaaaa
     8281 tcgccatatt gcgcttcttc acgcggtgcg gcgcgggtga acagcagcag gtattccgga
     8341 tcgtactcca gatccgggcc gcaggggcat tccgccgaaa ttgggtcaga caggaatatt
     8401 tccatttaat tatcctcagt gcgccagcgt ggtggccaga ccgtccggat tgctggactc
     8461 acgcaccaga tgggccgatg ccgccagacg agcgtgatct ggcaacgggc tgtcagcgct
     8521 ctcccgcaaa tcctgcccgc tgaggctggc gacataaaag gcaaagcggt catgttgcgg
     8581 tttatccaga ccgcccccag cggactgggc gctgcgccat gcctggtaaa actgcgtcag
     8641 ccagcggtga tgaaaaagat cagaaaaacg ggtcagcgtc ggctttcagt ccggttacgc
     8701 gccagttcgg tgtagtgcag cggcagtgga ccgttcggcc cccacagcac gaggctgtac
     8761 agactgatgt gcaggcgccc ttcctgccag cccacactgg cgatttctcg tggcgcaaag
     8821 gtcagcgcgg gcgtttgtcc gaggcgaaat ttttccatcc gtggttgcca ggtgttgccc
     8881 gccggaacgg tacacaactg cacatccacg cggcgcatca ggttcagaaa accgtaacgc
     8941 cagggggggg cctgcgcctg agtgaaatcg ttgctcatac gcttccccgc tgtcccgccc
     9001 tgacaggcca ggtcataatt ttgccgcgct gcatgctgtg cagcgtcatc tgcgagaagg
     9061 tattaatgga aacatggcgg gcgatatagt gttccagcac caggccgaac agatacggac
     9121 tgatgccgga aaaaccttct tcatccaccg tcagttcaca actgacgcca cgcccataga
     9181 ccagtagtcc ggaaccgggc agacggcggg tgaccggggt cgttttacaa ccaatcaggc
     9241 tgcgcacctg gcgcgattgc ggactgtcat gagcaggaat aaacaggttc agcagatctc
     9301 gcaacgcctg gccgccggta cggtgatcca gatcggccag cggcagataa ttaaaagata
     9361 actggcggat caaccgccat gccatctcac gttcagccat cgggggctgc ggcgagcgtg
     9421 gcgggcggat aagccctacg cccgccaccg ggatggctgc atccacggtt agatcgtccc
     9481 ggccattgcg ggcaataaga cagggcagat cgcggttagt gaccatggcc gtgatggtga
     9541 tgtggcgcag attttccggg tacggcgctt catactggtc aaccagcgac aggaacactt
     9601 ccgagcccgt atagggggta cgggtgccgt aacggcgggc gttttctgat aagcggcggg
     9661 gctcccggcg cagggaaaaa taacgtccgt gattgccttc atcattattg cgggtgtggt
     9721 agagcgggcg aaaggccatc ttccgtgtgg tgtcggtttc cagcccttcc acttcctgga
     9781 ccgagaacac ctcataatcc agcggatggg ttctgtccac caccagatgc tgttccgtcg
     9841 tgctgtgcgt gacatcgatc cgggcggtga cgcggggaaa caggttgata accggggtac
     9901 agaacaggct gaactgtgcc gcatttgtct ggtggatcag ccagtccggc ggcaggcggt
     9961 tcagcaggat aacaatctcc gccacgccac cgtcaatttt ctgtaatccg gcagacaggc
    10021 ctgtcggggt gaaaaaatag aagcgttccg gacaggcaaa atattcatgc agcaggttat
    10081 gaccgtgaaa cacgttccac gccagcggca gcagcccctg atccggctcc aggccttcgt
    10141 acataacggg ctgttgcaga ttcacatcca gcgcgccgtc aaaatgcccc ggaatgccag
    10201 ccaggggcgc gatggcgctg gtatgtagaa gttctaacag gtgtgaggcc gtacgctcct
    10261 cgccgcagag ataaaacggc agcctgtcca gtccggcaag ctgactgaac gttagttcac
    10321 caaaggtgcg cagggtaata cgcagcgccc cggcgacgtg aatgttggcc ggcaggtagc
    10381 ggtgcagggc aggcatatcc ggtggcgcgg cggtcagtcg cgcctcttcg atggcgagcg
    10441 gccacagcgt tacatcctgg ctgctgcgaa actggcaggc agtgctttcg ccttccggaa
    10501 tggcggagaa aaaggctgtg tcgcgcggga cggtaaatcc tttcgccaga tcgccttctt
    10561 ccgtatcggg atgcaactgc gccacgctca ttgagggcgt gggtgtgacg taattggggc
    10621 tgaccacttc cagcaggcgc tgggtgaaac gggggaattc agcgtcaatt tttagctgcg
    10681 tgcgggcggt caggaagctg aaggcctcga ccattcgctc tacatacgga tcggcaatat
    10741 ccgttccctg catgcccagc cgggcggcga ctttgggatg acgggcggca aattcggctc
    10801 ccgtttcccg cagatagctc agttcgcggt tgtagtattc cagtagccgt ggatccatcg
    10861 gtctctctct gaattaaaaa aacgtaatgc gactctgctc catatccagc gcgctacgta
    10921 cccggaatgc agtcggatac ggctgcgtga ggatctcgcc gcgaatttca aactgtaatg
    10981 tgttatggcc actctggcgg cgctcatcct gaagtgggat gatttgcaga gtggacgctt
    11041 tcagccgggg ttcaaaccgg gtgatggttc gcaaaatggc cctgcggata tcgtcccact
    11101 tgtgctcata cagaaagctg cccgccagtg gcggcaggcc gtagttaagt acggacgctg
    11161 ccgcgtacgg ataacgtgca gcatcaatat catcttcatg gctgacggtg ttgagcagaa
    11221 atgacaaatc ccggcggatg atctccttaa tctgaaccgg actgacgctg ataccgtggt
    11281 cgcgtttctg gtttggggcg tcgtcacaca ggcggtcaaa cagcgtgggc agcaggtgat
    11341 tgcctggacg ctggcgggaa gcgctcacgc gtcgcttcct tcagcggtgt taaaggtaca
    11401 ggtggacaaa tccagcaggc tgatatcgcc gtgaccggtg agccacaccc tctgacccag
    11461 cgcctgtacc agtgtctctc ccggaccgtc ctgccagacc gtttcccggc acagacgcag
    11521 ggagtcggca gccttttcag agccgctgta gcggggaaac agctaggcac tgtgtgtatc
    11581 gccattgttc agggtgacgt tgacgggttt ccagatcaaa tcgatcagat gcgcaggccg
    11641 cggcgatttc agcgaacaga tttgtgaaaa cggcagccag atatatgctc cgcccgcgat
    11701 cagttcgagt accggaccgg tacgggagtc gctgtcgcta atccagtcaa atgcgccgcc
    11761 attccactgc ccggaagtgt ctgttatggc ctcaagcgcg cggctgcggt gagcatcagc
    11821 ttcctgcgct tcaccacggg cgttacaggc gagggcggtc agcagatcct ccatccaggc
    11881 gggcggcggg agaataacac cgggccgctg ttcgccctga aagcaggcgt ggcggtatat
    11941 ctcacagcgg atcagctcgc caaatacctg cgcttcgcga gtataatttg cgtccatccg
    12001 ggcgcatagc tggatctgtt gcagtgcccg cgaccagtcg cctgccacgc acaataactg
    12061 aaacaggctg tggcggcggg aggcctgcgc tgggtgcatc ctgacctgtt gctctgccat
    12121 ctgaatgccc tccaccacgg aataatcccg cagcagcgcc gacaaggtaa tcggtagctc
    12181 gttattgttt gtcatgatat atctctccat gttgtgccgg gtttatccgg taatggctgt
    12241 caatgccaat gatgcgatgc tctttccgtg tcagatctgg cagtacggtg ttatgcgctg
    12301 tttccggggc gtattccggg gaaagtaaat gcaggatatc agggggagtc ttttcgacag
    12361 gccagatagt ttcatcttcg ccagtggcat ccagtacatt gagaatagcg tcaatgctta
    12421 acccaccagt gaccatatcc tgcaatgtct cggcttcggt gaccaggtca cgcaatgaat
    12481 ccagatcctg cgaggagggc tcttcgttaa aaggaaaagg atcagcatgg cgattaagat
    12541 cttgttcggg agataacaac gcctcgcggt attcctgata aagtcgggtc agggcatttc
    12601 ctttttccgc accttctccg gtggaggagg ctccatgaac aggaatgata tcgaatggat
    12661 tttgtgatgt cagatgctgc tgcctgaacc aatccagatc cagatattcg gttattgctt
    12721 ccgacgtgat ggcagtctgc gatatcgttt ttactgtgct caggttcgga gatgatggat
    12781 tgccatgtac aaggcactat gaagataacc cccattctat caggtcgcct tcattcagac
    12841 gcatacggtg atgaggttcc acaatctggt cgtttaccgt acagcaatat tcggtagact
    12901 gattgataat ccaccatgct tcttcgtggc gggtcagagt gagggcaatc tttacatcat
    12961 cattcacctt aaaaacagga atccagggag aggtaacggt gaagataatt tgtgttcctt
    13021 cagtcgcgta aattgtgtta tcggcgatcc cgtgcgcctg tattttttgg agtccccacg
    13081 taatatttgg tttcattgtg tatagactgg ccccctgaat ctccagacaa ccagtatcac
    13141 ttat
//
LOCUS       DAJTRD010000096        12810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTRD010000096 DAJTRD010000000
VERSION     DAJTRD010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.096"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..1177)
                     /locus_tag="OO315_003214"
     CDS             complement(<1..1177)
                     /locus_tag="OO315_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5892016.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     "
     gene            complement(1376..1460)
                     /locus_tag="OO315_003215"
     tRNA            complement(1376..1460)
                     /locus_tag="OO315_003215"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1424..1426),aa:Leu,seq:caa)
     gene            1656..2675
                     /gene="ahr"
                     /locus_tag="OO315_003216"
     CDS             1656..2675
                     /gene="ahr"
                     /locus_tag="OO315_003216"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5892017.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2804..4306
                     /locus_tag="OO315_003217"
     CDS             2804..4306
                     /locus_tag="OO315_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5892018.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="OO315_003218"
     CDS             complement(4425..5507)
                     /gene="lptG"
                     /locus_tag="OO315_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5892019.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="OO315_003219"
     CDS             complement(5507..6607)
                     /gene="lptF"
                     /locus_tag="OO315_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5892020.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6874..8385
                     /gene="pepA"
                     /locus_tag="OO315_003220"
     CDS             6874..8385
                     /gene="pepA"
                     /locus_tag="OO315_003220"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5892021.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8519..8962
                     /gene="holC"
                     /locus_tag="OO315_003221"
     CDS             8519..8962
                     /gene="holC"
                     /locus_tag="OO315_003221"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5892022.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            8962..11817
                     /gene="valS"
                     /locus_tag="OO315_003222"
     CDS             8962..11817
                     /gene="valS"
                     /locus_tag="OO315_003222"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5892023.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11871..>12810)
                     /locus_tag="OO315_003223"
     CDS             complement(11871..>12810)
                     /locus_tag="OO315_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR5892024.1"
                     /translation="AILMTVSADMPLVGCVLTLLLLVLLIFMAAKGLRYQALMTSLNG
                     VRFSFNCSLKGFWWVTFFLPILMAIGMGTVFFISTKMLHANSSSSVIISVVLMAIVGI
                     VSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDTAYCIKYAILAFLALLPFLAVAGY
                     IIFDQILNEYDSSGYANDDIENLQQFMEMQRKMIIAQLIYYFGIAVSTSYLTVSLRNH
                     FMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISGITGGLAYPLLKIWMIDWQAKNTY
                     LLGDLDDLPLINKEEQPDKGFLASISRGVMPSLPFL"
BASE COUNT         3242 a         3328 c         3373 g         2867 t
ORIGIN      
        1 acatcaacct acgctcccca agatgttccg ccttatggat gtaagctgct cgcacagagc
       61 tacgctcctg gtgactcatc tgccgctcta ccgcatccct cgaccataat cccgactcaa
      121 tcaacgaact acaagccatc gtcctgaagc catgcccaca aacctcgacc ttcgtgtcat
      181 accccattac gcgcaaagcc ttgttcaccg tattctcact catcggctta cgtggatcgt
      241 gatcgcccac aaaaatcagc tctcgatttc cgctcatgct tttgatcttt tccaaaatac
      301 ttagggcctg ccgcgacaaa ggtacaaggt gtggtgtccg catcttcgag ccacgctgag
      361 aatgcttgac tccttccagt tgctcacgct cacctgggat tgtccacata gccgtttcaa
      421 aatctatttc tgaccagcgg gcaaaacgca gctcgctcga acggatgaaa accaataaag
      481 tgagttcaac cgccagtcga gttaacggtc tgccagaata gtgatctatg cgatgaagta
      541 attcaggtat acggttcagt tcaagagctg cacggtgctg tcttttcgcc gtagcaaccg
      601 cacctgcaat ctcttgcgcg gggttgtagt cgattaagcc gctctgaacg gcaaagcgca
      661 taatcgcggt agtacgctgc tgtaaacggg cggcaacttc gagccgcccg gatgactcga
      721 ctgctttaat gggtacaaga agatcccgcg tcttcagttc cgcaatgttc cgcttaccga
      781 tagcagtaaa gagattatcc tccagacttt tcaaaacacg agcgctatgc gatgccgacc
      841 acttctgatt gctggcgtgc cagtctctgg cgactacttc aaacgttatc gcctcttgtt
      901 cctgttctac cttaacagct ttcttgtttt cactgggatc gacaccatta gctaaaagct
      961 tacgagcctc atcccggcgt gccctggcat ccgccaacga cacttcgggg tatttcccca
     1021 atgccagcat cttctctttg ccgccaaagc gataacgtag ccgccagtat ttggagccgt
     1081 tagggtgaac cagcaaaacc atgccttcac cgtcggtcag cttataggct tttgcttcag
     1141 gcttagccga acgaaccttc acatcactca gagccatgat gagtatcctt tcaagggttc
     1201 tgtgtgggta caaacattat cgaaccggga aatacccgca gttgtacccg catcagtaag
     1261 ttgatgtaga ttgaatcagg ttgacttagg ttgagtgaaa aagcgaggaa agccttgcgg
     1321 atactggatt ttaggcacaa aaaaagacgt ccgttgacgt ctattgatgt tccgatggtg
     1381 ccgaaggccg gactcgaacc ggcacgtatt tctacggttg attttgaatc aactgcgtct
     1441 accgatttcg ccacttcggc actgaagagg tatgcggaaa acgttgtgga ttatacctgt
     1501 cgcacgccac catgcaagcg ccagcttgca cccacctcgc taagtgctga aattttcagc
     1561 attacactca gcgatatgta acctttgtca cactccaggc accccgccct gccatgctct
     1621 acacttccca aacaacacca gagaaggccc caaaaatgtc gatgataaaa agctatgccg
     1681 caaaagaagc gggcggcgaa ctggaagttt atgagtacga tcccggtgag ctgaagccac
     1741 aagatgttga agtgcaggtg gattactgcg ggatttgtca ttccgatctg tcgatgatcg
     1801 ataacgaatg gggattttca caatatccgc tggttgccgg gcatgaggtg attgggcgcg
     1861 tagtggcact cgggagcgcc gcgcaggata aaggtttgca ggtcggtcag cgtgtcggga
     1921 ttggctggac ggcgcgtagc tgtggtcact gcgacgcctg tattagcggt aatcagatca
     1981 actgcgagca aggtgcggtg ccgacaatta tgaatcgcgg tggctttgcc gagaagttgc
     2041 gtgcggactg gcaatgggtg attccactgc cagaaaatat tgatatcgag tccgccgggc
     2101 cgctgttgtg cggcggtatc acggtcttta aaccactgtt gatgcaccat atcactgcta
     2161 ccagccgcgt tggggtaatt ggtattggcg ggctggggca tatcgctata aaacttctgc
     2221 acgcaatggg atgcgaggtg acggccttta gttctaatcc ggcgaaagag caggaagtac
     2281 tggcgatggg tgccgataaa gtggtgaata gccgcgatcc gcaggcactg aaagccctgt
     2341 cggggcagtt tgatctcatt atcaatactg tgaacgtcag cctcgactgg cagccttatt
     2401 ttgaggcgct gacctatggc ggtaatttcc atacggtcgg tgcggttctc acgccgctgt
     2461 ctgttccggc ctttacgtta attgcgggcg accgcagcat ctctggttct gctaccggca
     2521 cgccttatga gctgcgaaag ctgatgcgct ttgccgcccg cagcaaggtt gcgccgacaa
     2581 ccgaactgtt cccgatgtcg aaaattaacg acgccattca gcatgtacgc gatggtaaag
     2641 cgcgctaccg agtagtcctg aaagccgact tctgatccct ttgccgggtg ctgtcgcccg
     2701 gcacttaaat ctctttggaa ggcgtctcgc aatctgctga atttagcggt gaagagtgca
     2761 cagccgttgc ctatactcca ggcaggaaac tggaggaaat ctcatgagcg aacccctgtt
     2821 aattgcccgc acgccggaca cagaactgtt tttactgccg ggaatggcta accgtcacgg
     2881 gctgattact ggcgcaacgg ggacgggtaa aaccgttacg ctgcaaaaac tggcagagtc
     2941 attgtcggaa atcggcgtgc cggtgtttat ggctgatgtg aaaggcgatc tgaccggtat
     3001 cgcgcaggca ggaacggcgt cggaaaaact gctcacaagg cttaaaaata tcggcgtcaa
     3061 tgactggcaa ccgcatgcca atccggtggt ggtgtgggat atctttggcg agaaaggcca
     3121 cacggtgcgg gcgacggttt cggatctggg gccgctgttg ctggcgcgac tgttgaatct
     3181 caacgatgtg caatctggcg tgctgaatat tatcttccgc attgcggacg atcagggatt
     3241 gttgctgctc gactttaaag atttgcgggc gattacccag tacatcggcg ataacgccaa
     3301 atccttccag aatcagtacg gaaatatcag tagcgcatcg gttggtgcca tccagcgcgg
     3361 attactgtcg ctggaacagc aaggcgcagc acacttcttt ggtgagccga tgctggatat
     3421 caaagactgg atgcgcaccg atgccaacgg taaaggcgtt atcaatatcc tcagcgccga
     3481 gaaactttat cagatgccga aactgtacgc cgccagcctg ctgtggatgc tttcagagtt
     3541 gtatgaacaa ttgccggaag cgggcgatct ggagaaacca aaactggtgt ttttcttcga
     3601 cgaggcacat ctgctgttta acgatgcacc gcaggtactg ctggataaga ttgagcaggt
     3661 gataaggctt attcgctcaa aaggcgtggg cgtctggttc gtttcgcaaa acccgtctga
     3721 tattccggat aatgtgttcg gccagctcgg taatcgtgtt cagcacgctt tgcgggcttt
     3781 tacgccaaaa gatcagaaag cagtgaagac agcggcgcaa accatgcggg ccaatccggc
     3841 atttgatacc gaaaaggcaa tccaggaact ggggaccggc gaggcgttaa tctcgtttct
     3901 cgatgcaaaa ggaagtcctt ctgtggtgga acgggcgatg gtgatcgcac cttgttcgcg
     3961 aatggggccg gtgacggaag atgagcgtaa tggcctgatt aatcactccc cggtgtatgg
     4021 caaatatgaa gatgaggtgg accgcgagtc cgcctatgag atgctgcaaa aaggctttca
     4081 ggccagtacc gagcagcaaa ataatccccc cgtgaaaggg aaagaggtgg cggtggatga
     4141 cggtattctt ggtggattga aggatatttt gtttggcact accggaccac gcggcgggaa
     4201 gaaagatggt gtggtgcaaa caatggcgaa aagcgccgct cgccaggtga cgaatcagat
     4261 tgtacgcggg atgttgggga gtttgctggg ggggagaaaa agataagtca gacgtgatct
     4321 gtgcccctca ccctaaccct ctccccaaag gggcgagggg accgttcgaa ctcgtatttg
     4381 actttgtcaa caataataaa gggagctttc gctcccttgt tagattacga ttttctcatt
     4441 aacagccaca ggctgattaa gaagaagctg gcacttggca acagtgcgcc gatgatcggc
     4501 gggatgccgt aaaccaacgt cagcgggccg aagatctggt ccagtacgta gaagacaaaa
     4561 ccgaagctga tacctgtgac cacacgcacg cccatcggta cgctacgcag tgggccaaag
     4621 ataaacgaca gcgccatcag catcatcacc gccacggata gcggctggaa gattttgctc
     4681 cacatgttga gctgataacg tccggcatcc tgaccgctcg acttcagata cttcacgtag
     4741 ttgtgcaaac cactgatgga gagtgcatcc gggtccagcg ccaccacgcc cagtttgtct
     4801 ggcgtgaggt cggttttcca ggtgccgctc accgtctgcg aaccggtaat ctgtttcgga
     4861 ttggtcagat cagattcatc aacctgcgac agacgccaga ctttatgttc cgggtcaaac
     4921 ttcgcggtag cggcataacg tacggattgc agacgacgat tctcgttaaa ggcataaata
     4981 ctgatgccac ctaacacctc gtcaccttta acccgctcaa tgtagacgaa gttgttgcca
     5041 tctttcgccc ataaaccttg ctgggtagag agcaacgagc cgccgtacat cgcctgcgca
     5101 cggtagttac gcgccatctg ttcgccctgc ggcgcgaccc attcgccaat cgccatcgtc
     5161 agcaagacca gtggaatggc ggttttcatc accgacagcg ctacctgcat acgggtaaaa
     5221 ccagaagcct gcatcaccac cagttcgctg cgctgcgcca gcatcccaag accaagcaac
     5281 gccccaagaa gagccgccat cgggaagaag atctgcacat ctttcggcac gctcagcaag
     5341 gtatacattc ctgcgcctaa cgcgtcgtaa ctcccctgcc cggctttttt cagctgatcg
     5401 acaaatttga taatgcccga cagcgacacc agcatgaaca gcgtcatcat gatggtggtg
     5461 aaaatagttt taccgatata gcggtcaagt acgccaaaag gttgcatcac accgctcctt
     5521 tacgcgaaaa actggcgcgc aggcggcgga ccggcacggt gtcccaaagg ttgagaacaa
     5581 tcgccaaagc cagataaatc aggttaacgg tccacatcca cagtgtcggg tccagcttac
     5641 ctttaccgcc gttcgatttc agggaggtct ggatcaggaa gaaaagtaga tacagcagca
     5701 tggctggcag catcgacagt acgcgtccct gacgcgggtt aaccacgctc aacggtacaa
     5761 ccataagtgc catcataaac acggtgaata ccaacgtgat acgccagttc agttctgcgc
     5821 gagcacgatc ggtgtcagtg ttccacaatg tgcgcatgtc catctggtcg gtatcgttcg
     5881 ggtcgagcgc caccgcctgg tgaccaatga tcgcctgata atcctggaaa tccgtaatgc
     5941 ggaaatcacg taacagtgca gtgccttcga agcgcgttcc ctggttgaga gtgacgacct
     6001 gggagccgtc gcgcagctgg gttaaatgtc cggaatcggc caccaccaca gaaggacgtg
     6061 cattaccttt tggtcgaatt tgtgcgagga acacatcttt gaaatcgctg ccgtcaacgc
     6121 tttcgatgaa cagcaccgag ctgccattag tcgcttgctg gaattgccct tgcgccagcg
     6181 ccgccatgcc agggttcgct ttcgcttctg ctaacacttc atcctgatga cgcgatgacc
     6241 acggtcccgc ccacatcacg ttaaccgccg cgacgattgc cgtgaatacc gcaaggatca
     6301 ttgccgcttt caccagaacc gctttgctca agccgcaggc atgcattacc gtaatttcac
     6361 tttcggtata cagtttgccc agcgtcatca gcagcccgag gaacaggctt aatggcagga
     6421 taagctgcgc catttccggc acgcccaacc cgagaaggga gagcaccaga ttcgccggaa
     6481 tatcgccgtc aaccgctgcg ccgaggatcc tcactaactt ttgacagaag aagatcaaaa
     6541 gcaagatgaa gagtatcgcc agctggcttt tgagcgtctc ccgcaccaga tatcttatga
     6601 ttatcacttt aaatacgccc gtaaaaactc gtcttttgca ggattttagc ttgtttcatg
     6661 gcttaaacgt catttattct cttgagtcgt cgaaatcgtc gctaagataa ttatactcaa
     6721 cggattcacc tctcagattt tgttctgacg tgccaatgcc gtaataacgt taagattaac
     6781 acgaagtcat cgcaacagcg gacatgagtt acgaaagctt gcaattctat ctgtagccac
     6841 cgccgttgtc tttaagattc aggagcgtag tgcatggagt ttagtgtaaa aagcggtagc
     6901 ccggagaaac agcggagtgc ctgcatcgtc gtgggcgtct tcgaaccacg tcgcctttct
     6961 ccgattgcag aacagctcga taaaatcagc gatgggtaca tcagcgccct gctacgccgg
     7021 ggcgaactgg aaggaaaacc ggggcagaca ttgttgctgc accatgttcc gaatgtactt
     7081 tccgagcgaa ttctccttat tggttgcggc aaagaacgtg agctggatga gcgtcagtac
     7141 aagcaggtta ttcagaaaac cattaatacg ctgaatgata ctggctcgat ggaagcggtc
     7201 tgctttctga ctgaactgca cgttaaaggc cgtaacaact actggaaagt gcgtcaggct
     7261 gtcgagacag caaaagagac gctctacagt ttcgatcagt tgaaaacgaa taagagcgaa
     7321 ccgcgtcgtc cgctgcgtaa aatggtgttc aacgtgccga cccgccgtga actgaccagc
     7381 ggtgagcgcg cgatccagca cggtcaggcg attgccgccg ggataaaagc agcgaaagat
     7441 ctcggtaata tgccgccgaa tatctgtaac gccgcttatc tcgcctctca ggcgcgccag
     7501 ctggctgaca gctacagcaa gaatgtcatc acccgcgtta tcggcgaaca gcagatgaaa
     7561 gagctgggaa tgcactctta tctggcggtc ggtcagggtt cacaaaacga atcgctgatg
     7621 tcggtgattg agtacaaagg caacgcgtcg gaagatgcac gcccaatcgt gctggtgggt
     7681 aaaggtttaa ccttcgactc cggcggtatc tccatcaagc cttcagaagg catggatgag
     7741 atgaagtacg atatgtgcgg cgcggcggca gtttacggcg tgatgcgtat ggtcgcggag
     7801 ctgcaactgc cgattaacgt tattggcgtg ctggcaggct gtgaaaacat gcctggcggg
     7861 cgtgcctatc gtccgggcga tgtgttaacc accatgtccg gtcaaaccgt tgaagtgctg
     7921 aacaccgatg ccgaaggccg cctggtactg tgcgacgtgt taacttacgt tgaacgtttt
     7981 gagccggaag cagtgattga cgtggcgacg ctgaccggtg cctgcgtgat cgcgctgggt
     8041 catcatatta ctggtctgat gacgaaccat aatccgctgg cccatgaact gattgccgcg
     8101 tctgaacaat ccggtgaccg cgcatggcgc ttaccgctgg gtgacgagta tcaggaacag
     8161 ctggagtcca attttgccga tatggcgaac attggcggtc gtcctggtgg ggcgattacc
     8221 gcaggttgct tcctgtcacg ctttacccgt aagtataact gggcgcacct ggatattgca
     8281 ggaaccgcct ggcgttctgg taaagcaaaa ggcgcaaccg gtcgtccggt agcgttgctg
     8341 gcacagttcc tgttaaaccg cgctgggttt aacggcgaag agtaagtttg cgttttaccc
     8401 tcaccctaac cctctcccta agggagaggg aaccgatcga gcgcaataga aattcattgc
     8461 tcatctttca gggtgaggaa tgcagcaata tttaaatcca ccacaagaag ccccatatat
     8521 gaaaaacgca acgttctacc ttctggacaa tgacaccacc gtcgatggct taagcgccgt
     8581 tgagcaactg gtgtgtgaaa ttgccgcaga acgttggcgc agcggtaagc gcgtactcat
     8641 cgcctgtgaa gatgaaaagc aggcttaccg gctggatgaa gccctgtggg cgcgtccggc
     8701 agaaagcttt gttccgcata atttagcggg agaaggaccg cgcggcagtg cgccggtgga
     8761 gatcgcctgg ccgcaaaaac gcagcagcag cccgcgcgat atattgatta gtctgcgaac
     8821 aagctttgca gattttgcca ccgctttcac agaagtggta gacttcgttc cttatgaaga
     8881 ttctctgaaa caactggcgc gcgaacgcta taaagcctac cgcgtggctg gtttcaacct
     8941 gaatacggca acctggaaat aatggaaaag acatacaacc cacaagatat cgaacagccg
     9001 ctttacgagc actgggaaaa gcagggctac tttaagccta atggcgatga aagccaggaa
     9061 agtttctgca tcatgatccc gccgccgaac gtcaccggca gtttgcatat gggtcacgcc
     9121 ttccagcaaa ccatcatgga taccatgatc cgctatcagc gcatgcaggg caaaaacacc
     9181 ctgtggcagg tcggtactga ccacgccggg atcgctaccc agatggtcgt tgagcgcaag
     9241 attgccgcag aagaaggtaa aacccgtcac gactacggcc gcgaagcttt catcgacaaa
     9301 atctgggaat ggaaagcgga atctggcggc accattaccc gtcagatgcg ccgtctcggc
     9361 aactccgtcg actgggagcg tgaacgcttc accatggacg aaggcctgtc caatgcggtg
     9421 aaagaagttt tcgttcgtct gtataaagaa gacctgattt accgtggcaa gcgcctggta
     9481 aactgggacc cgaaactgcg taccgctatt tctgacctgg aagtggaaaa ccgcgaatcg
     9541 aaaggttcga tgtggcacat ccgctatccg ctggctgacg gcgcgaaaac cgcagacggt
     9601 aaagattatc tggtggttgc gaccactcgc ccggaaaccc tgctgggcga taccggtgta
     9661 gctgttaacc cggaagatcc gcgttacaaa gatctgattg gcaaatatgt cattctgccg
     9721 ctggttaacc gtcgtattcc gatcgttggc gacgaacacg ccgacatgga aaaaggcacc
     9781 ggctgcgtga agatcacacc ggcgcacgac tttaacgact atgaagtcgg taaacgtcac
     9841 gccctgccga tgatcaacat cctgaccttt gacggtgata tccgtgaaag cgcccaggtg
     9901 ttcgatacca aaggtaacga atctgacgtt tattccagcg agatcccggc agagttccag
     9961 aaactcgagc gttttgctgc acgtaaagca gtcgttgctg cggttgatgc actcggcctg
    10021 ctggaagaaa ttaaaccgca cgacctgacc gttccttacg gcgaccgtgg cggcgtggtt
    10081 atcgaaccga tgctgaccga ccagtggtac gtgcgtgccg atgtactggc gaaaccggca
    10141 gttgaagcgg ttgagaacgg cgacattcag ttcgtaccga agcagtacga aaacatgtac
    10201 ttctcctgga tgcgcgatat tcaggactgg tgtatctctc gtcagctgtg gtggggtcac
    10261 cgtatcccgg catggtatga cgaagcgggt aatgtttatg ttggccgcaa cgaagaagaa
    10321 gtgcgcaagg aaaataacct cggtgccaac gttgccctgc gtcaggacga agacgttctc
    10381 gacacctggt tctcttctgc actgtggacc ttctctaccc ttggctggcc ggaaaatacc
    10441 gacgccctgc gtcagttcca cccaaccagc gtgatggtat ctggtttcga catcatcttc
    10501 ttctggattg cccgcatgat catgatgacc atgcacttca tcaaagatga aaatggcaaa
    10561 ccgcaggtgc cgttccacac tgtttacatg accggtctga ttcgtgacga cgaaggtcag
    10621 aagatgtcca aatccaaggg taacgttatt gacccgctgg atatggttga cggtatttcg
    10681 ctgccagaac tgctggaaaa acgcaccggc aatatgatgc agccgcagct ggcggacaaa
    10741 atccgtaagc gcaccgagaa acagttcccg aacggcattg agccgcacgg caccgatgcc
    10801 ctgcgcttca ccctggcggc gctggcgtct accggtcgtg acatcaactg ggatatgaag
    10861 cgtctggaag gctaccgtaa cttctgtaac aagctgtgga acgccagccg ctttgtgctg
    10921 atgaacacag aaggtcagga ttgcggcttc aacggcggcg aaatgacgct atcgctggcg
    10981 gaccgctgga ttctggcgga gttcaaccag actatcaaag cgtaccgcga agcgctggac
    11041 agcttccgct tcgatatcgc cgcaggcatt ttgtatgagt tcacctggaa ccagttctgc
    11101 gactggtatc tggagctgac caagccggta atgaacggtg gaacagaagc cgaactgcgc
    11161 ggtactcgcc atacgctggt gactgtactg gaaggtctgc tgcgcctcgc gcatccgatc
    11221 attccgttca tcaccgaaac catctggcag cgtgtgaaag tactttgcgg tatcactgcc
    11281 gacaccatca tgctgcagcc gttcccgcag tatgatgcat ctcaggttga tgaagccgca
    11341 ctggccgaca ccgagtggct gaaacaggcg atcgttgctg tacgtaacat ccgtgcagaa
    11401 atgaacatcg cgccgggcaa accgctggag ctgctgctgc gtggttgcag cgcggatgca
    11461 gaacgtcgcg taaatgaaaa ccgtggcttc ctgcaaacgc tggcgcgtct ggaaagcatc
    11521 accgtgctgc ctgccgatga caaaggtccg gtttccgtta ccaagatcat cgacggcgca
    11581 gagctgctga tcccgatggc tggcctcatc aacaaagaag atgagctggc gcgcctggcg
    11641 aaagaagtgg cgaagatcga aggtgaaatc agccgcatcg agaacaagct ggcaaatgaa
    11701 ggctttgtcg cccgcgcacc ggaagcggtt atcgcgaaag agcgtgagaa gctggaaggt
    11761 tatgcagaag cgaaagccaa gctgattgaa cagcaggctg ttatcgccgc actgtaatca
    11821 acacagtgat gaaaacgaag gccggagcat gctccggcct tttttatctc ttacagaaat
    11881 ggtaaagaag gcataactcc ccgagaaata ctggctaaga agcctttatc tggttgttct
    11941 tctttattga ttaaaggaag gtcatccaaa tcgcccagca aatacgtatt ttttgcctgc
    12001 cagtcaatca tccatatttt cagcagtggg taagccagac cacccgtaat cccggatatc
    12061 accaccaacg cacacatgcg ataaagcata ccgtggtacg ttaaagttga gcgaaaacga
    12121 atacgcccat cattcagtga caggttgctc ataaaatgat ttcgcaacga caccgttaaa
    12181 taactggtgc taacagcaat cccaaaataa taaattaact gcgcgattat cattttacgt
    12241 tgcatttcca taaattgctg taaattctca atatcatcat ttgcataccc agaactatca
    12301 tactcattta atatttgatc gaagataata taaccagcaa cagcgagaaa aggtaataaa
    12361 gctaaaaatg cgaggatggc atattttata caatacgcag tatctaattt caccttgaaa
    12421 cgatgtatac cgaaactggt attgctccag agaaaactca ttaccaggct atataaagta
    12481 ccattaaaaa taccaatgga aacaatacca actattgcca tcagaaccac agatataata
    12541 acactacttg aactattggc atgtagcatc tttgtcgaga taaagaaaac agtccccatc
    12601 ccaatggcca ttaaaatcgg caagaaaaag gttacccacc agaacccttt caaagagcaa
    12661 ttaaaactaa atcttacgcc gttgagactg gtcattaagg cctgataacg cagtcctttt
    12721 gctgccataa atataagcaa aaccaacagt aacaaagtca aaacacagcc aacaagcggc
    12781 atatctgctg acactgtcat taagattgcg
//
LOCUS       DAJTRD010000097        12703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTRD010000097 DAJTRD010000000
VERSION     DAJTRD010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.097"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(107..745)
                     /gene="rutR"
                     /locus_tag="OO315_003224"
     CDS             complement(107..745)
                     /gene="rutR"
                     /locus_tag="OO315_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5892025.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1033..2124
                     /gene="rutA"
                     /locus_tag="OO315_003225"
     CDS             1033..2124
                     /gene="rutA"
                     /locus_tag="OO315_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5892026.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2124..2816
                     /gene="rutB"
                     /locus_tag="OO315_003226"
     CDS             2124..2816
                     /gene="rutB"
                     /locus_tag="OO315_003226"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5892027.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2828..3214
                     /gene="rutC"
                     /locus_tag="OO315_003227"
     CDS             2828..3214
                     /gene="rutC"
                     /locus_tag="OO315_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5892028.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3222..4022
                     /gene="rutD"
                     /locus_tag="OO315_003228"
     CDS             3222..4022
                     /gene="rutD"
                     /locus_tag="OO315_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5892029.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4032..4622
                     /locus_tag="OO315_003229"
     CDS             4032..4622
                     /locus_tag="OO315_003229"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5892030.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4633..5127
                     /gene="rutF"
                     /locus_tag="OO315_003230"
     CDS             4633..5127
                     /gene="rutF"
                     /locus_tag="OO315_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5892031.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5148..6476
                     /gene="rutG"
                     /locus_tag="OO315_003231"
     CDS             5148..6476
                     /gene="rutG"
                     /locus_tag="OO315_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5892032.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6558..6665)
                     /locus_tag="OO315_003232"
     CDS             complement(6558..6665)
                     /locus_tag="OO315_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892033.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7038..7634
                     /gene="wrbA"
                     /locus_tag="OO315_003233"
     CDS             7038..7634
                     /gene="wrbA"
                     /locus_tag="OO315_003233"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5892034.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7655..7882
                     /locus_tag="OO315_003234"
     CDS             7655..7882
                     /locus_tag="OO315_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5892035.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7920..9161)
                     /gene="agp"
                     /locus_tag="OO315_003235"
     CDS             complement(7920..9161)
                     /gene="agp"
                     /locus_tag="OO315_003235"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5892036.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9454..10710)
                     /locus_tag="OO315_003236"
     CDS             complement(9454..10710)
                     /locus_tag="OO315_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5892037.1"
                     /translation="MGSNIHGISCTANNYLKHAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10971..11891
                     /gene="cbpA"
                     /locus_tag="OO315_003237"
     CDS             10971..11891
                     /gene="cbpA"
                     /locus_tag="OO315_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5892038.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11891..12196
                     /gene="cbpM"
                     /locus_tag="OO315_003238"
     CDS             11891..12196
                     /gene="cbpM"
                     /locus_tag="OO315_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5892039.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12289..>12703)
                     /gene="torD"
                     /locus_tag="OO315_003239"
     CDS             complement(12289..>12703)
                     /gene="torD"
                     /locus_tag="OO315_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5892040.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3035 a         3241 c         3201 g         3226 t
ORIGIN      
        1 tgaacgttcg ctgagtgtga ataccgctgc cgctggcggt aacgccagtt taatgacgat
       61 cggttaaaca ggtagccgga ggatgttaca acctcctccg tcatctttaa cgtggtcgaa
      121 tcccctcaat aataatccgc tgcacgtttt caaccgtttg attgaaaaat acctcatcgc
      181 gcaacgtcgc gcctgtcacc gcctccacct gaggggcgaa atcggcgtaa tgttgagtgg
      241 aagcccaaat cataaaaatc aaatgctgcg gatcaatcgg cgcgagtttg ccgcttttga
      301 cccaaccggc aatcagcgcc gatttctcat caattaatgc cttcaaatcg cccgtcagtt
      361 catccattaa caacggcgcg cctgccagca tctccataca gaacaggcgc gaagcctgcg
      421 gataatcgcg tgagacttcc agcttcagac ggatgtactc ttttatcgcc gccagcgggg
      481 cgaaatcttc acgaaacgct tttaacggtg ccagccagat atcgagaatc tgccgcagca
      541 cggcaatata cagcgcctct tttgacggga agtaatacag cagattggtt tttgaaacac
      601 ccgccaactc tgcgatctgc tccagccttg tgccgtgaaa accgaattgt gaaaaagtgt
      661 ccagtgctgc gctaagaatc gcttttttct tcgcgcttac tgcgcgcgaa cgtttacccg
      721 ttgttttcac tgcgccttgc gtcatgcgct ctcccctctt gtagattggt aacaggatag
      781 caaaaccccc gcccggcctc gaccttctgc actctcatcg cgcacggatg catgttttat
      841 gtgcaactgt tttgaccgtt tagtccactt tttaccagat attttagata acacaatgct
      901 aacacattaa taatattcac tttttaaaaa ctggcatccg ctttgcgaac aagccaatac
      961 gcggctgaaa aggatatgca ggatgcagcg ccccgtctga ctttcacttt acgagacgaa
     1021 gagaggttga tgatgaaaat tggcgtattc gtacctattg gcaacaacgg ctggctcatt
     1081 tcgacccacg cgccgcagta catgccgacc tttgaactga ataaagccat tgcgcaaaaa
     1141 gcggagcatt accatttcga tttcgccctg tcgatgatca aactgcgtgg ctttggcggc
     1201 aaaactgagt tctgggatca caaccttgag tcgttcacct tgatggcggg tctggcagcc
     1261 gtgacctcgc gcattcagat ttacgccact gctgccacct taacgttacc tccggcaatc
     1321 gtcgcccgta tggccgcaac catcgactcc atctctggcg ggcgttttgg cgtcaacctc
     1381 gtgactggct ggcaaaagcc cgagtatgag cagatgggta tctggcctgg cgatgactat
     1441 ttctcccgtc gttacgacta tctcaccgaa tatgttcagg tgctgcgcga cctgtggggc
     1501 acggggaaaa gcgattttaa aggcgatttt ttcaccatga atgattgtcg cgtcagtccg
     1561 caaccgagta tccccatgaa agtgatctgc gccgggcaaa gcgacgctgg catggcgttc
     1621 tccgcccagt atgccgattt caacttctgt ttcggcaaag gcgtaaatac acccacggct
     1681 ttcgccccga ccgctgcgcg gatgaaacag gccacagagc aaaccggacg cgacgttggc
     1741 tcttatgtgt tgtttatggt gattgccgat gaaaccgacg atgccactcg cgccaaatgg
     1801 gaacactaca aagcgggcgc ggatgaagag gcgttaagct ggctaaccga acaaagtcag
     1861 aaagataccc gctccggtac tgacaccaac gttcgtcaga tggccgatcc cacttcggcg
     1921 gtaaacatca atatggggac gttagtcggt tcttacgcca gtgtcgcgcg catgttagat
     1981 gaagtcgcaa gcgtgcctgg tgcagaaggc gtgctgttga ccttcgatga ttttctgtcg
     2041 ggaatcgaaa ccttcggcga gcgcattcaa ccactgatgc agtgccgcgc ccatctctct
     2101 gcgctgactc aggaggtggc atgatgacaa ccttaaccgc tcgaccggaa gccattacct
     2161 tcgatccgca gcaaagtgcg cagatcgtgg tggatatgca aaacgcttat gccacgccag
     2221 gcggctactt agatctcgcc gggtttgatg tctcaaccac tcgcccggtc attgccaaca
     2281 ttcaaaccgc tgtgaccgca gcgcgagcgg cagggatgtt gatcatctgg tttcaaaatg
     2341 gctgggatga acagtatgtc gaggctggcg gccccggctc accgaatttt cataaatcga
     2401 acgccctgaa aaccatgcgt aagcagccgc agctgcaggg gaaattgctg gcgaaaggct
     2461 cctgggatta tcaactggtg gatgaactgg tgccgcagcc tggcgatatt gtgctgccga
     2521 agccgcgcta cagcggtttc ttcaatacgc cgctggacag cattttacgc agccgtggaa
     2581 tacgccatct ggttttcacc ggtatcgcta ccaacgtctg cgtcgaatcg acgctacgcg
     2641 atggcttttt tctggagcat ttcggcgtgg tgctggaaga cgcaacacac caggcggggc
     2701 cggaatttgc acagaaagcc gcgttgttca atatcgaaac cttttttggc tgggtcagcg
     2761 acgtcgaaac gttctgcgac gcgctttctc ccacgtcctt tgctcgtatc gcttaaggag
     2821 tttagcgatg ccaaaatccg taattattcc cgctggcagc agcgcaccgc tggccccctt
     2881 cgttcccggc acgctggctg atggcgtggt gtatgtctcc ggtacgctgg cttttgatca
     2941 acataataac gtgctgtttg ccgatgaccc aaaggcgcaa acccgccacg ttctggaaac
     3001 tatccgcaag gtgatcgaga cggcgggtgg cacgatggcg gatgtgacct tcaacagcat
     3061 ctttattacc gactggaaaa attacgccgc gattaacgaa atctacgccg agttttttcc
     3121 gggtgataaa ccggcgcgat tctacattca gtgcggactg gtaaaacctg acgcgctggt
     3181 ggaaatcgcc acaattgcgc atatcgccaa gtgaggccgc gatgaaactt tcactctcat
     3241 ctccccctta tgctgatgca cccgtagtgg tgttgatttc gggtcttggc ggtagcggca
     3301 gttactggtt accgcaactg gcggtgctgg agcaggagta tcaggtagtc tgttacgacc
     3361 agcgcggcac cggcaataat cccgacacac tggcagaaga ttacagtatc gcccagatgg
     3421 cagcggaact gcatcaggcg ctggtagccg cagggattga gcgttacgcg gtggtcggcc
     3481 atgcgctcgg tgcgctggtg gggatgcagc tggcgctgga ttatcccgcg tcggtaacta
     3541 tgctggtcag cgttaacggc tggctacgaa taaacgccca tacgcgccgc tgttttcagg
     3601 ttcgcgaacg attactgtat agcggcggcg cgcaagcatg ggtggaagcg cagccgttgt
     3661 tcctctatcc cgccgactgg atggcggccc gcgcacctcg cctggaggca gaagacgcgc
     3721 tggcactggc gcattttcag ggcaaaaata atttactacg tcgacttaac gccctcaaac
     3781 gcgctgactt tagtcaccat gcggatcgca tccgctgccc ggtgcaaatc atctgcgcca
     3841 gtgatgatct gctggtgcca acagcatgtt ccagtgaact tcatgccgcc ctgcccgata
     3901 gccagaaaat ggtgatgccc tatggcggac acgcctgcaa cgtgaccgat cccgaaacgt
     3961 ttaatgctct gttactcaac gggcttgcca gcctgttaca tcaccgtgaa gccgccctgt
     4021 aaggaattgc tatgaacgaa gccgttagcc caggtgcgct tagcaccctg ttcaccgatg
     4081 cccgcactca caacggctgg cgggagacac ccgtcagcga tgagacgtta caggagcttt
     4141 atgccctgat gaaatggggg ccgacatcag ctaactgttc tccggcacgg atcgtgttta
     4201 tccgcactgc agaaggaaaa gaacgtctgc gcccggcgct ttccagcggc aatctgcaaa
     4261 aaaccctgac cgcgcccgtc accgctatcg tcgcctggga cagtgaattt tatgaacggt
     4321 taccactact gtttccccac ggtgatgccc gcagttggtt tacctccagc ccacaacttg
     4381 ccgaagaaac agcgtttcgc aacagttcca tgcaggcggc ttatctgatc gtcgcttgcc
     4441 gtgcgctggg actggatacc ggcccgatgt cgggttttga ccgtcaacac gttgacgacg
     4501 ccttttttgc gggcagcacg ctgaagagca atctgctgat taatatcggc tatggcgata
     4561 gcagcaaact ttttgcgcgc ctgccacgtc tgtcctttga agaagcctgc gggctgttgt
     4621 aaggagcaaa ctatgaacat agttgatcaa caaacctttc gcgatgcgat gtcctgtatg
     4681 ggcgcggcgg tcaatatcat caccacggac ggtccagccg ggcgcgccgg gttcaccgcc
     4741 agcgccgtct gcagcgtgac cgacacgccg cccacattac tggtgtgcct gaatcgtggg
     4801 gcgtccgtct ggccggtatt caatgaaaat cgaacgctgt gtgtaaatac actgagcgcc
     4861 gggcaggagc cgctttcaaa tctttttggc ggcaaaacgc ccatggaaca ccgctttgcc
     4921 gccgcccgct ggcagacggg tgtgaccgga tgcccgcaac tggaagaggc tctggtttcg
     4981 tttgactgcc gtatcagcca ggtggtgagc gtcggcaccc acgacattct gttttgcgcc
     5041 atcgaagcga ttcatcgtca caccacaccc tacgggctgg tgtggttcga tcgcagttat
     5101 cacgcgctga tgcgccctgc ttgttaacct ccttaaggag acagctgatg gcaatgttcg
     5161 gttttcctca ctggcagtta aaatcgacct ctacagagag cggcgtggtc gcgccggatg
     5221 aacgattgcc gtttgctcag acggcagtaa tgggcgttca acacgcggtg gcgatgtttg
     5281 gcgcgacggt gttaatgccg attctgatgg ggctggatcc caatctttcc attttaatgt
     5341 cggggatcgg cacgttactg tttttcttta ttaccggcgg gcgcgtcccc agttatctcg
     5401 gctccagcgc ggcttttgtc ggcgtggtga ttgccgcgac gggctttaac ggtcagggca
     5461 ttaacccgaa tatcagcatc gcgctgggcg ggattatcgc ctgtgggctg gtttataccg
     5521 tcattggcct ggtggtgatg aaaattggta cacgctggat tgaacgacta atgccgccgg
     5581 tggtgacggg tgcggtggtg atggcgattg gcctgaactt agcgcctatc gcagtgaaaa
     5641 gtgtatcggc ttcggccttt gatagctgga tggcggtaat gacggtgctg tgtattgggc
     5701 tggtggcggt gtttacccgt ggcatgatcc agcggttatt gattctcgta ggattaattg
     5761 tcgcctgtct gctgtatggc gtgatgacca acgttttagg gctgggaaaa gccgtcgatt
     5821 ttactctcgt cagctatgct gcctggttcg gtctgccgca tttttccact cctggtttta
     5881 atggccaagc gatgatgttg attgcgcccg tggcggtgat tctggtggcg gaaaacttag
     5941 gtcatctcaa ggcggtcgcc gggatgaccg gacgcaatat ggacccgtat atggggcgag
     6001 cattcgtcgg tgatggtctg gcaacgatgc tttccggctc tgtcggcggt agcggcgtca
     6061 ccacctatgc tgaaaatatc ggtgtgatgg ccgtgacaaa agtctactcg acgctggtgt
     6121 ttgttgcagc tgccgtcatc gctatgctgt tgggcttttc gccgaaattt ggcgcgctga
     6181 tccataccat tccggccgcg gtgattggtg gtgcatcaat tgttgtgttc gggctgattg
     6241 ccgtcgcagg cgcaaggata tgggtacaaa accgtgtcga tttaagccag aacgctaatt
     6301 tgattatggt cgcagtaacc ttcgtgctgg gcgcgggtga ttttgctctc acgcttggcg
     6361 gttttacgtt gggagggatc ggtacagcaa cctttggcgc gattttactc aatgcgttat
     6421 taagcagaag gttggttgac gttccaccgc cagaagttgt tcatcaggag ccataaacag
     6481 aaaaccgccg ggttaccggc ggtgatgact ttttgacgag cttaagaaaa aaatgcattc
     6541 gtcattgatt agcccggcta gttgtcgctt ttgccgtgac tgctcttgcc acctttttta
     6601 cctgcttctg atgctctttc gcggtcttct gcaaaattgc cggaaccgcc tcgatggttt
     6661 gccattactg acctccgtct gggtatagac atcatattgc atagaattaa cgcggcgtat
     6721 ggcggctgcc atattttcgc tacgtgggac taagcttagt gaactgacgc ggacagaccc
     6781 gcgtttggta atatcctgca acagcttctt agcgagaata gatatcatct ccaataatta
     6841 tccataagcc gctcttatga ttaaaaaaac gcgatgagat aaaaatgtgt cattttgcga
     6901 caaaattacg tgcttgtgaa agttgttata aatcaaataa gtggttgtga aatttgcact
     6961 ctgaaaagga cgtcttatct ttaaataagt ggtagcgaat cgctacggaa tagagataac
     7021 acgaggagtg gttagaaatg gctaaagttc tggtgcttta ttattccatg tacggacata
     7081 ttgaaacgat ggcacgcgca gtcgctgagg gtgcaagcaa agtggatggc gcagaagttg
     7141 tcgttaagcg tgtaccggaa accatgccgc cgcaattatt tgaaaaagca ggcggtaaaa
     7201 cgcaaactgc accggttgca accccgcaag aactggccga ttacgacgcc attatttttg
     7261 gtacacctac ccgctttggc aacatgtccg gtcaaatgcg taccttcctc gaccagacgg
     7321 gcggcctgtg ggcttccggc gcactatacg gaaaactggc gagcgtcttt agttccaccg
     7381 gtactggcgg cggtcaggaa caaaccatca catccacctg gacgaccctt gcgcatcacg
     7441 gcatggtgat tgtccccatt ggctacgcag cgcaggaatt atttgacgtt tcacaggttc
     7501 gcggcggtac gccgtacggc gcaaccacca tcgcaggcgg tgacggctca cgccagccaa
     7561 gccaggaaga actgtctatt gctcgttatc aaggggaata tgtcgcaggt ctggcagtta
     7621 aacttaacgg ctaatcttca acaggaggat acgcatgcca actcaagaag cgaaagctca
     7681 tcacgtcggt gaatgggcat ctctgcgcaa tacgtcgccg gaaatagccg aggccatttt
     7741 cgaagtcgcc gggtacgacg aaaaaatggc agaaaagatt tgggaagaag gtagtgatga
     7801 agtgttagtc aaagcctttg ctaagaccga taaagattcg cttttttggg gcgaacagac
     7861 catcgaacgt aaaaacgttt aaccagcgac tcccccgctt ctcgcggggg agttttctgt
     7921 tatttcaccg cctcattcaa cacgctatca aacttatcca tcgggcagaa accgttggca
     7981 tctatcgggc aaccgcttaa ttccagcgtc acgcgctgcg caggtgcctg cagggttaac
     8041 gcatcggcat tacgtaactg ttccgcactc tggtacacat attcaatttt catcaaatcg
     8101 cgattggctt tgctgtcatg ccaacgctgg aaaacgattt tgccgccaat cggcgtgcgt
     8161 tcgttctggt catgcaactg atacggtttg aaatccagcg ccgttaacag agaggctatg
     8221 ttggagtcgt gcccaactaa caccgtgatt ttcggagcac tggcgcgatc ggtgaccagg
     8281 gctttgtcga tataactgac cagcggtttc gcaacattgc gcgccacttc cggtgaggta
     8341 aacagactgt cctggtaacc gtttttcagc ttcgacaata ccttccactg ctggtcagat
     8401 ttgatttctc cccaggccac ctgatccatc ggaaaacctt cgtagtattg caaagtaaac
     8461 gcatctacca gcgagttgcc gactttcagc ggcccggaaa cacctggttc ttgttgatac
     8521 ttggcgctaa aggtattttt gccatccacc agcgaacact gttgtttctc tttacaggca
     8581 ggggaatctt tatagttaac gattttttcc agtagctggt agctgtcggt aagctggagt
     8641 ttgctgagct ctttctccat tgccgccacc gcctgttcac tgaatgcggc ggaatcatcg
     8701 gtgatcatcg ggttaaaggt tgggtccata gtgcccattt tttcctggtg atgcacagga
     8761 atatcacacc cagggaatgc accagtaata aagaactggg cggtagcgac ggtacgttgc
     8821 agactattgg cataggcata aacggtgtcc ggcggcgggc attcccccga tttcactatc
     8881 ccctgctctg ccagccattc acgcatgtaa tggcccatat acacttcgag cacgccacct
     8941 ttggtggtga gttgcccacc ggggacgtcc cattcgggcc atttattcgg cgtcgactgc
     9001 tccagcacac tgccattgtt cgccagcggc gcacgtaagt tatggcggct catcatgagc
     9061 acttgctgta gctgatagcc ttccggtacg gtttgtgcct gagcatttga agcgagtaaa
     9121 actatccctg ccacagttgc ggcgattagc gttttgttca ttcctggcac ctcttttgtt
     9181 atcaatcatt aaagagtgtg acagaaatat gacttttttc cgggaacagg ttcgcaaaat
     9241 ggcttgttga acgcctgttt ttctatatac atcttcaaaa acaggcaagg tttgacgcaa
     9301 actatgcctg attagcccct gcctcgcgta cgccgctgat aattgcacct gtggctaaac
     9361 agaatatctg cggcgttacc aatacagaga caatatttac caataaaaaa gtgtgagact
     9421 aataacaatg gtttttagca tatgcctttg ccattaagtt gcataacgat ttaaatagtc
     9481 aagttgcgca ttcatttcct tattttttaa cttataagcc tgagaaaaaa gttcaggagg
     9541 gaggtaaaca tcataatgat tgccagtgtt tgttaaatat ccatactcaa gatcttcatc
     9601 tgttgaaaag gattcatttc ccaaggagat tgatgccaca ttactattaa caataaataa
     9661 tgagcaaatg ctatctgaaa gaacagcaag aaaattcttc accgctttaa tcctatccga
     9721 cgtgtcggta tctaaagcaa gcaaattctt gttatcatta aatataaact cgggtaaatg
     9781 cagtatgcat tttttgtcat tcaagcaact atcaatgaat tgttttttaa aaaacagaaa
     9841 gctccatgac ataaaactac ccacaccctg aattttgttg tcagattgca gatcaacgcc
     9901 aaataatgtc tttacatctt ccctgagatt atagaatgcc gtatcaacaa gagcctcgat
     9961 cgtttgttta tcaacaattt taataaactc ttcgtatact tgcttttgcg tcttatggct
    10021 gcccagcgcc catgaatact catgttcttt taaaaccagt atggctctta acagacaata
    10081 tccatcgcca tctactggag cgtattttaa tccatatgtt ttattaattt cactctgaat
    10141 aaaagatatg ccagacaaat caataccagt atctaaatca gatattcccg gcatatttat
    10201 ctgttctggc tggatactca tattttcatt atctaaacaa tactcagaaa aaacatcatc
    10261 acacgcatta atgttttcaa tatattccat aaagccatac caataataac ctttaaaatc
    10321 tgtgatagac tctgaacctc tgcccggcaa ggacactcta acaatatcaa catcttgaac
    10381 atattgtata gtatatgtaa tacctcctga aacccctgaa taaatttgtg tatcattagt
    10441 acgcaatgtt ctcataaata atgatagatc attatcattc tgcatttcct caaattcttt
    10501 ttctagtgat tcacattcca gacatggagt atcctcttca tttactttac gtctgagttc
    10561 attgtacaac cgcataaaac acaccagtgt gttttcaaaa agcgtgattg aatatgtttg
    10621 atttttttcg tactcatttt ttatatcgtt ccaggcatgt ttcaaataat tatttgcagt
    10681 gcaactaatt ccatgtatat tactacccat atatagcgtc tataaaattt aataaataat
    10741 gacgccctag ttaaacttaa agtgcctggt tcaactatca aaaatcgctc accctttttc
    10801 acctgtttaa aatatgttca gcaacccatc ttgatggcga cctcctctcc gcgatgattt
    10861 caataacata ttctgtgttg gcatatgaaa ttttgaggat taccctacac ttataggagt
    10921 taccttacag gggttccttc aatttgtgtt gatttacgcg agataacgct atggaattaa
    10981 aggattatta cgccatcatg ggcgtgaaac cgacggacga tctcaagaca atcaagaccg
    11041 cctatcgtcg actggcccgt aaataccatc ctgatgtcag caaagaaccg gatgccgaag
    11101 cccgcttcaa agaggtcgct gaagcctggg aagtgttaag tgatgaacaa cgtcgcgctg
    11161 agtatgatca gatgtggcaa catcgcaacg atccgcaatt taaccgtcag ttccatcatg
    11221 gcgacggtca gagttttaat gccgaagatt ttgacgatat cttctcgtca attttcggtc
    11281 agcatgcccg ccagagccgt caacgccccg ccgcacgtgg ccacgatatt gaaatcgaag
    11341 tggcggtatt cctcgaagaa acgcttactg agcataagcg taccatcagc tataacctgc
    11401 cggtttataa cgcctttggc atgatcgaac aggaaatccc gaaaacgctg aatgtgaaga
    11461 tcccggcagg cgtcggcaat ggtcaacgca tccgcctgaa aggccagggg acgccgggcg
    11521 aaaacggcgg tccaaatggc gatttgtggc tggtgattca tattgcgcca catccgctgt
    11581 tcgatattgt cggccaggat ctggaaattg tggtgccggt tagcccgtgg gaagcggcgc
    11641 tgggtactaa agtcaccgtt ccaacactga aagaaagcat tttgctgact atcccgccag
    11701 gcagccaggc cgggcaacga ttgcgcgtta aaggcaaagg tctggtgagc aaaaaacaga
    11761 ccggcgatct gtatgcggta ctgaaaatcg tgatgccgcc gaaaccggat gaaaacactg
    11821 ccgcgctgtg gcagcaactg gcagacgccc agtcgtcttt tgatccacgt aaagattggg
    11881 ggaaagcata atggctaatg ttacggtgac ttttactatt accgaatttt gcctgcatac
    11941 cggcatctct gaagaggagt tgaatgaaat tgtcggtttg ggggtggttg aaccgcgtga
    12001 gatccaggaa acaacctggg tatttgacga ccatgccgcc attgtggtgc aacgcgcggt
    12061 acgcctgcgt catgaactgg ctctggactg gccggggatc gcggtggcgc tgacgttaat
    12121 ggatgatatt gcgcacctga agcaggaaaa ccgcctgcta cgccagcggc tttcccggtt
    12181 tgtagctcat ccgtgatgtg tgtttgccgg atgtgatgac gcatccggca actgcgccat
    12241 aatgcctgat gcgacgctaa acgcgtcttt tcaggcgcat aaacgacgtt atctgttttg
    12301 gtggtcgcac tccactaaca ccagcaataa ctggcttagt gccgcgtaaa aaccaaagct
    12361 gtcatactgt cgacaacgcg cagcaaactc tggtaaccat tgccgcagcg ccgtcagtgt
    12421 tttttgccgc aaactgtcga ttcttcgcgc aggaacggtc ccctctccca gcgaaaaatg
    12481 cagataactc aacaactcga gatagatcgc cagatgatct gccggttcgt tgaaattgcc
    12541 actggtttcc atccctgcct caactaacaa gcgtttaatc tcttgctcgt cctgtttgta
    12601 ggccgatgca tacggcagcg ccgcttgttt gtcggtcatc agaaacaggc cgcaaaagtc
    12661 agcggccagt tccagacggg catcgtcacg tactgtcagc gtg
//
LOCUS       DAJTRD010000098        12654 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTRD010000098 DAJTRD010000000
VERSION     DAJTRD010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.098"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            130..1827
                     /gene="maeA"
                     /locus_tag="OO315_003240"
     CDS             130..1827
                     /gene="maeA"
                     /locus_tag="OO315_003240"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5892041.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1961..2971
                     /gene="adhP"
                     /locus_tag="OO315_003241"
     CDS             1961..2971
                     /gene="adhP"
                     /locus_tag="OO315_003241"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5892042.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3117..3401
                     /locus_tag="OO315_003242"
     CDS             3117..3401
                     /locus_tag="OO315_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5892043.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3629..4282)
                     /gene="fdnI"
                     /locus_tag="OO315_003243"
     CDS             complement(3629..4282)
                     /gene="fdnI"
                     /locus_tag="OO315_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5892044.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4275..5159)
                     /gene="fdnH"
                     /locus_tag="OO315_003244"
     CDS             complement(4275..5159)
                     /gene="fdnH"
                     /locus_tag="OO315_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5892045.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5172..8219)
                     /gene="fdnG"
                     /locus_tag="OO315_003245"
     CDS             complement(5172..8219)
                     /gene="fdnG"
                     /locus_tag="OO315_003245"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7632..7634),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5892046.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8451..9332
                     /gene="yddG"
                     /locus_tag="OO315_003246"
     CDS             8451..9332
                     /gene="yddG"
                     /locus_tag="OO315_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5892047.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9587..10687
                     /gene="ompC"
                     /locus_tag="OO315_003247"
     CDS             9587..10687
                     /gene="ompC"
                     /locus_tag="OO315_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5892048.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFIRAVDIRTDNIVATGITYQF"
     gene            10851..11336
                     /locus_tag="OO315_003248"
     CDS             10851..11336
                     /locus_tag="OO315_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5892049.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11446..12435
                     /locus_tag="OO315_003249"
     CDS             11446..12435
                     /locus_tag="OO315_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5892050.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLILSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3337 a         3124 c         2941 g         3252 t
ORIGIN      
        1 tgacgattaa acaccattgc ctgcgcaatg gtgtttttat ctgctttata cttggggacg
       61 acgccctggc ggtaaagcaa agacgataaa agcgtgccag ggatggatat tcaaaaaaga
      121 gtgagtgaca tggaaccaaa aacaaaaaaa cagcgttcgc tttatatccc ttacgctggc
      181 cctgtactgc tggaatttcc gttgttgaat aaaggcagtg ccttcagcat ggaagaacgc
      241 cgtaacttca acctgctggg gttactgccg gaagtggtcg aaaccatcga agaacaagcg
      301 gaacgagcat ggatccagta tcagggattc aaaaccgaaa tcgacaaaca catctacctg
      361 cgtaacatcc aggacaccaa cgaaaccctc ttctaccgtc tggtaaacaa tcatcttgat
      421 gagatgatgc ctgttattta taccccaacc gtcggcgcag cctgtgagcg tttttctgag
      481 atctaccgcc gttcacgcgg cgtgtttatc tcttaccaga accgccacaa tatggacgat
      541 attctgcaaa acgtgccgaa ccataatatt aaagtgattg tggtgactga cggtgaacgt
      601 attctggggc ttggtgacca gggcatcggc gggatgggca ttccgatcgg taaactgtcg
      661 ctctataccg cctgtggcgg catcagcccg gcgtataccc ttccggtggt gctggatgtc
      721 ggaacgaaca accaacagct gcttaacgat ccgctgtata tgggctggcg taatccgcgt
      781 atcactgacg atgaatacta tgaattcgtt gatgaattta tccaggctgt gaaacaacgc
      841 tggccggacg tgctgttgca gtttgaagac tttgctcaaa aaaatgcgat gccgttactt
      901 aaccgctatc gcaatgaaat ttgttctttt aacgatgaca ttcagggcac tgcggcggta
      961 acagtcggca cactgatcgc agccagccgc gcggcaggtg gtcagttaag cgagaaaaaa
     1021 atcgtcttcc ttggcgcagg ttcagcggga tgcggcattg ccgaaatgat catcgcccag
     1081 acccagcgcg aaggattaag cgaggaagca gcgcggcaga aagtctttat ggtcgatcgc
     1141 tttggcctgc tgacggacaa gatgccgaac ctgctgcctt tccagaccaa actggtgcag
     1201 aagcgcgaaa acctcagtga ctgggatacc gacagcgatg tgttgtcact gctggatgtg
     1261 gtgcgcaatg taaaaccaga tattctgatt ggcgtctcag gacagaccgg gctgtttacg
     1321 gaagagatca tccgtgagat gcataaacac tgtccgcgtc cgatcgtgat gccgctgtct
     1381 aacccgacgt ctcgcgtgga agccacaccg caggacatta tcgcctggac cgaaggtaac
     1441 gcgctggtcg ccactggcag cccgtttaat cctgtggtat ggaaagataa aatctaccct
     1501 atcgcccagt gtaacaacgc ctttattttc ccgggcatcg gcctgggtgt tattgcttcc
     1561 ggcgcgtcac gtatcaccga tgagatgctg atgtcggcaa gtgaaacgct tgctcagtat
     1621 tcgccgctgg tcctgaacgg cgaaggtctg gtactaccgg aactgaaaga tattcagaaa
     1681 gtctcccgcg caattgcgtt tgcggttggc aaaatggcgc agcagcaagg cgtggcggtc
     1741 aaaacgtctg ctgaagcttt gcaacaagcc attgacgata atttctggca agccgaatac
     1801 cgcgactacc gccgtacctc catctaagcc tgcacccggt agtgaaggct accgggctat
     1861 ttccctctcc ctttttcaga tctcatccat actgggtagt ggcgaataaa tctcatttgc
     1921 ctcacctgct atgcagaaca tcatccgaaa aggaggaact atgaagactg cagttgttac
     1981 gaaggatcat catgttgacg ttacggataa aacactgcgc tcactgaaac atggcgaagc
     2041 cctgctgaaa atggagtgtt gtggtgtatg tcataccgat cttcatgtta agaatggcga
     2101 ttttggtgac aaaaccggcg taattctggg ccatgaaggc atcggtgtgg tggcagaagt
     2161 tggcccaggt gtcacctcat taaaaccagg cgatcgtgcc agcgtggcgt ggttttacga
     2221 aggatgcggt cattgcgaat actgtaacag tggtaacgaa acgctctgcc gttcagttaa
     2281 aaatgccgga tacagcgttg atggcgggat ggaggaagag tgcatcgtgg tcgccgatta
     2341 cgcggtaaaa gtgccagatg gtctggactc ggcggcgccc agcagcatta cctgtgcggg
     2401 ggtcaccacc tacaaagccg ttaaactgtc aaaaattcgt cccggtcagt ggattgctat
     2461 ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcaaagaatg tctttaacgc
     2521 caaagtgatc gccattgatg tcaatgatga gcagttaaaa ctggcaaccg aaatggatgc
     2581 agatttagcg attaactcac gcaccgaaga cgccgccaaa attgtgcagg agaaaaccgg
     2641 tggcgctcac gctgcggtgg taacagcagt agctaaagct gcgtttaact cggcagttga
     2701 tgctgtccgt gcaggcggtc gtgttgtggc tgtcggtctg ccgccggagt ctatgagcct
     2761 ggatatccca cgtcttgtgc tggatggcat tgaagtggtc ggttcgctgg tcggcacgcg
     2821 ccaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc cgaaagtcgc
     2881 cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag gcaaaatccg
     2941 tggccgtatg gtgattgatt tccgtcacta agaggtcttt gctgcgaccc ccatgttcgg
     3001 gtcgcagcat cgcacactct ccaacatgaa atggctaaat ggattattgt tgcgtaggaa
     3061 ggaacaataa tgatagtgtt cccagagaag taacgctact gagagtaaag acactaatga
     3121 aaatggccaa tcatccccgc ccgggggaca ttattcagga atcactggac gaacttaatg
     3181 tcagcctgcg cgagtttgcc agagcaatgg aaattgcgcc ctcaacggca agccgattgc
     3241 tgaccggaaa agcagctttg acgccagaaa tggcaattaa actttccgtg gtgatcggca
     3301 gttcgccgca aatgtggctg aatctgcaaa acgcctggag tctggcagag gcagaaaaaa
     3361 cggtggttgt gtcgcgatta cgccgtctgg ttacgcaata aaaaatggca ccctataaag
     3421 cgccatttga aactgatgac aaacgcaaca ttgcctgatg cgctacgctt atcaggccta
     3481 cacgttcact tcaatctatt gtatttgaag ggttttcatg ccggataagg cattcacgac
     3541 gcatccggga tgaacaaagc ccacgttgtc tacaatatga aaatggcgcc ttttacagcg
     3601 ccattttcag cttaaagttc aaaagagatt atatcccttc ttcactctct tttttcgctt
     3661 ctgccttctc gatttcacga taccagcgcg gatggtgttt cttcgcccag cgacggctca
     3721 ccttcccttc gatcatccct ttaatcgacc ctttcaccca gaatgccata tacatatgga
     3781 tcaggatggc gtggatcagg atgatacccg cagccgcgtg gatcagcagg ctgtagcgaa
     3841 caacctgcat cgggaagtac tgcgcaaagt acggacgcca gataatcacc ccggtcacca
     3901 gcagcacgaa aatcatgctc atgatcgacc agaacatcat cttttgcccg gcgttgtact
     3961 taccaacatc cgccactttg tgctcattgc cttttaatac ttcgacaatg ttcaacagcc
     4021 acggaatatc tttcttatcc gggatgttgt gatgcacaaa acgcacaaac ataaacatca
     4081 gtgcgacgaa aatcgcaatg ccgaagaagg ggtgcaaaat gcgtcccatc tgcggcgtac
     4141 cgaaggtttg cgtcagccat tgcagcgtcg ggaagaagaa cgaaatcccg gacagcgcca
     4201 ccaggaagaa gcaaatcacc acggtccagt gacaggcgcg atcaataaat ttggtgcgca
     4261 caatcatttt cgacttactc atgatgatcc tcctcgtcat cgtccacttc cttattcggg
     4321 ccaataccga tgtagtggaa aatcaacccg gcaaaagtgg caataaagcc agccgctgcc
     4381 agcggtttca acgcgccttt ccacaggctt accgaggtgt cgatcttcgg atctttcggc
     4441 agaccgtgat acagctccgg ctgatcggcg tgatgcagca cgtacataac gtgcgtacca
     4501 ccgacccctt ccgggttgta gacgccagca tgttcgtaac cacgcgcttt cagtttcgcc
     4561 acgcgctgtt ccgccagctc cagcatctcc ttcttggtgc cgaagtggat agccccggtc
     4621 ggacaggttt tcacacaagc cggttcctgg ccgacgctga cgcgatcgac gcagagcgtg
     4681 catttatata cccggttatc ctctttgttg aggcgcggaa tattaaacgg acacccggca
     4741 atgcagtaac cacagccgat gcagttttcc gactggaaat cgacaatccc gttagcgtac
     4801 tgaatgattg caccagcaga cgggcacgcc ttcaggcagc ccggatcttc acagtgcata
     4861 cagccgtctt tacggatcag ccactccagc ttgccattct gttcggtttc gctaaagcgc
     4921 atcaccgtcc aggacttggc gctcagatcg gcggggttat cgtaaacccc gacgcagtgc
     4981 cccacttcat cacggatgtc gttccactcc gaacacgcca cctgacaggc tttacagccg
     5041 atacaggtgg aaacgtcgat aagtttcgcg acttctgctt tgtaatcacg cacctgagaa
     5101 ggcggcgtga tggagttagt tgcggacctt ttgataatat cctgcgtttc catagccatc
     5161 tgttcgcccc cttacgcctt ctcgatgttg actaagaacg ctttatattc cggcgtttgc
     5221 gagtttgcat caccgacatt cggcgtcaga gtgttagcga tataaccttt acgcgcgaca
     5281 ccctcaaagc cccagtggat tggaataccc accgtttcaa cctgctgacc gtttacattc
     5341 agcggtttca gacggcgcgt taccactgcc accgcgcgga taaagcctcg cttgctggag
     5401 acagtgacac gatcgccatt attaatgcct ttcgccgccg cctgcgtttc gctgatttcc
     5461 acaaactgtt ccggctgagc aattgcgttg agcaacgcgt gcttggtcca ggtgtggaag
     5521 tgctcggtca gacgataggt cgtacccaca tacgggaact gctctttttt acccatccgc
     5581 agcgcgtctt gttcatacag acgaacaacc gggttagaca ccacgttcgg atgcagcggg
     5641 ttagtgccca gcggcgtttc aatcggctcg tagtgttccg ggaacggacc ttccgccatt
     5701 ttgttgatgg caaacaggcg tcccatccct tccggctgca taataaacgg cccggttggc
     5761 gtacccggtg cggcattgcc gaagtccgga atatcgttac ccgtccactt gctgccgttc
     5821 cactggatca gcatccgttt cggatcccac ggtttaccgt tgatatccgc cgaagcacgg
     5881 ttgtagagta cgcgacggtt gagcggccac gcccaggccc atcccagcgt attccccaga
     5941 ccggacgggt cggagttatc gcggtttgcc atctggttac cctgctctgt ccagctaccg
     6001 gtgtagatcc agcaagaaga tgcggttgta ccgtcatcac gcagatgcgc aaagctactc
     6061 agcaactgac ctttcttcgc aatcagcacg ccattagcat catagagatc ttccaacgca
     6121 tagccgttgt tctctttagc tacttcgtca gattgcggtt cgtgcggctg cttgtagttc
     6181 cagctcatct tcatcagcgg ttctacgcct ttaccacctt cggcctggta caactcgcgc
     6241 agatgatggt agatacccgc cagaatttcg ccgtcgttac gcgcttcgcc cggcgcgtcc
     6301 tgacctttcc agtgccactg caaccagcga ccggagttag cgatagaacc atcctcttca
     6361 gcaaagcagg tcgaaggcag acggaatact tcagtctgaa tagacgccgg atcgacatcg
     6421 ttcgactcgc cgtggttctg ccagaaggta gaggtttcag tcaccagcgg atcgataacc
     6481 accatgtact tcagtttgct caggcagctc accactttgt ttttatccgg gaaggacgca
     6541 accgggttaa agccctggca gaaataaccg gtaactttgc cttcatccat catgttgaaa
     6601 tacttgatga cgtcgtaggt ctggtcccac ttcggcagcc agtcatagcc ccagttgttc
     6661 tctttctgcg cggcatcgcc atagaaagat ttcatcaggc taacgaagaa cttcggatag
     6721 ttgctccagt agttcacctg atcagccagc gtcgctttcg gcgtgttcgc ttccagatac
     6781 gactgcaaat caacctgttt ttctgacggc agcgtcagat aacctggcag gctggtagag
     6841 agcagaccta agtcagtcag cccctgaatg ttggagtgac cacgcaatgc gttcacgccg
     6901 ccaccggcca tacccatgtt accgagcagc aactggatca tcgccatagt acggatgttc
     6961 tgcgcaccca cggtgtgctg cgtccagccc agcgcgtaca ggaaggtggt tgtgcgatcc
     7021 ggtgcgctgg tggaggccag cacttcacac actttcagga agtcggcttt tggcgtacca
     7081 cagatgtttt caacaacgtc cggcgtgtag cgggaaacgt gcgctttcag caggttccac
     7141 acacagcgcg gatgagtcag tgtttcatcg cgtttcgcat agccgttttc atcgaactga
     7201 tagttccagg acgatttgtc gtactggcgt ttttcagcgt cataaccgct gaacaggcca
     7261 tcttcgaaag caaaatcatc acgcaccagt aggctggcgt tggtgtaatg tttaacgtat
     7321 tcggcgttga ttttgttgtt ttcgatcagg tagcgcaaaa tgccagacag gaacgtaatg
     7381 tccgtaccgg aacgaatagg tgcgtaaata tccgccacag aagcggtacg cgtaaaacgg
     7441 ggatcgacaa cgatcaaggt tgcatcgttg ttgtttttcg cttccatcgc ccagcggaaa
     7501 ccgacgggat gcgcttcagc agcgttaccg cccattacca ttacgacgtt agcgtttttg
     7561 atatccaccc agtggttggt catagcaccg cgaccaaatg ttggagcaag acttgctacc
     7621 gttggtccgt gtcagacgcg cgcctggttg tctaccgcca gcatcccgag ggagcgggca
     7681 aatttctggg tcagcatccc ggtttcgttg ctggcaccgg aggcacacag cataccggta
     7741 gaaagccaac ggtttaccgt tacgccctgc tcgttctttt caataaagtt agcgtcacgg
     7801 tcagctttca tcagcttcgc aatacgggag aatgcttctt cccagctaat gcgctgccat
     7861 ttgtcagaac ctggcgcacg atatttcggg tagcgcagac ggttttcact gttgacgtaa
     7921 tccagcaaac cggccccttt cgggcacagc gcaccacggc ttaccggatg atccgggtca
     7981 ccttcaatgt gataaatcgc ttctctggcg ttttttgcgc catcacccag gctatacatc
     8041 aatagcccgc aacctacgga acagtatgtg caggtgttac ggatctcttt agcgcgtaat
     8101 aatttgtagt ttcgcgcctg agccagtgct tgcttcgggg caaagcccaa tgccgctacc
     8161 gttgttccag ccataccgcc cgcgcagatt ttaaaaaatt gtctgcgact gacgtccatt
     8221 gctttcctct tttttcaggg gtattactgc gcgaggaaac taacagtaaa attctgaatc
     8281 tttttgcgcg aaatcaaaaa gaacacgtgt ttgccacaaa ataatccgcc tgcgcaaatt
     8341 gacctacctc aatagcggta gaaaaacgca ccactgcctg acaggccagt taaaaaaatg
     8401 ctataaaatt cagcttaatt tttaacggca agagagacaa aacagcgagc atgacacgac
     8461 aaaaagcaac gctcataggg ctaatagcga tcgtcctgtg gagcacgatg gtaggattga
     8521 ttcgcggtgt cagcgagggg ctcggcccgg tcggcggcgc agctgctatc tattcattaa
     8581 gcgggctgct gttaatcttc acggttggat ttccgcgtat tcggcagatt ccgaaaggct
     8641 atttactcgc cgggagtctg ttattcgtca gctatgaaat ctatctggcg ctttccttag
     8701 ggtatgcggc gacccgtcat caggcgattg aagtgggtat ggtgaactat ctgtggccca
     8761 gcctgacaat tctctttgcc attctgttta atggtcagaa aaccaactgg ctgattgtac
     8821 caggattatt attagccctg gtcggcgtct gctgggtgtt aggcggtgag aatgggttac
     8881 attatgatga aatcatcaat aatatcacca ccagcccatt gagttatttc ctggcgttta
     8941 ttggtgcgtt tatctgggca gcctattgca cagtaacgaa taaatacgca cgcggattta
     9001 acggaattac cgtttttgtc ctgctaacgg gagcatgtct gtggatttac tattttctca
     9061 cgccacaacc agaaatggta tttagcacgc ccgtcatgat taaactcatc tctgcggcat
     9121 ttaccttagg atttgcttat actgcatgga atgtcggtat attgcatggc aatgtcacca
     9181 taatggcggt aggctcgtat tttacgcctg tactttcctc agcgcttgca gccgtgctgc
     9241 tcagcgcccc gctgtcgttc tcgttctggc aaggcgcact aatggtctgc ggcggttccc
     9301 tgctctgctg gctggcgaca cgtcgtggtt aaaataattg ccgggtctac ggcccggcag
     9361 ttaatcagat aaattccgac aatattaaaa aataaatttc aaataccagt aattcacctt
     9421 atatctcatt tcgacttcga tcacatatcg ataaataata ttattctatt tttctcattg
     9481 tttttaatca ccattctatt ttaaaaacag tatgtcattt ataaatgacg ccgtttaaaa
     9541 ttcgtttaga aaatacgttt aacgataatt aaaaggattc tgttaaatga aattaaaaat
     9601 agttgcggtg gttgtaactg gtttgttagc tgcgaacgta gcacacgctg ccgaagtcta
     9661 taacaaggat ggtaataaac tcgaccttta tggcaaggtt accgctctac gttattttac
     9721 tgatgataag cgtgacgatg gtgataaaac ttatgcccgt ctcggcttta aaggagaaac
     9781 gcaaatcaat gatcaaatga ttggttttgg tcactgggaa tatgatttta aaggctataa
     9841 cgatgaagcc aacggctcgc gcggcaacaa gacccgtctg gcctatgctg gtttaaaaat
     9901 tagtgaattt ggctctctgg actatggtcg taactacggt gtcggctacg acattggttc
     9961 atggaccgat atgttgccag aatttggtgg cgatacctgg agtcagaaag atgtcttcat
    10021 gacataccgt accaccggtc tagcaaccta tcgcaactac gatttctttg gcttaattga
    10081 agggctgaac tttgccgcgc aatatcaagg caaaaataaa cgtactgaca acagtcatct
    10141 ttatggtgct gacgacacgc gtgccaacgg tgacggtttc ggtatctcct caacttatgt
    10201 ttatgatggc tttggtatcg gtgcggtata taccaaatcc gatcggacaa atgcgcagga
    10261 aagagccgca gctaatcctc tcaatgcctc cggtaagaat gcagaactat gggccacagg
    10321 tataaagtat gatgccaaca acatctactt tgcagctaat tacgatgaaa cattaaacat
    10381 gaccacctat ggggatggtt atatctctaa caaagcacaa agttttgaag tggtggcgca
    10441 atatcaattc gacttcggct tgcgcccatc actcgcttac ctgaaatcga aaggcagaga
    10501 tctgggccgc tacgccgacc aggacatgat tgagtatatc gacgttggtg cgacgtattt
    10561 cttcaacaaa aatatgtcga cctatgttga ttataaaatc aacctgattg atgaaagcga
    10621 ctttatccgt gccgtagata ttcgcaccga taacatcgtc gctacgggca ttacctatca
    10681 gttctaacgt cctctcctga ccagcccttc tctgggctgg tttttgattt aagtgctgct
    10741 gtatgactcc ttaagtaaaa atgagcaatt cttgatattt ctggtcattc acctcaatta
    10801 atattattcc atatcctccg gggacatctt actataatat ttattgatga gtgattatga
    10861 ccaatattaa tacagcttgt gtaaaaaata atgccagtta tcaacttaat aacgcattac
    10921 ctaacaaaga aaccatctcc agcaattttt gtgaacgact agaacaatgg ggtaataaat
    10981 cggttaataa tggtgaagaa agagcaattg ccgtggagcg aattaaagaa gcttacaatt
    11041 cgaacatggc atctcttgat ttgtcatatc ttgatttgag tgaattaccg cctatcccct
    11101 cgacagttaa tacgttaaat ctggaaaaca attgtctaac ttgtcttgac ttagctgaca
    11161 atgccagtct cgttaatatc aacctcagcg ttaataaaat taacacgata acctttccaa
    11221 aagaaccaaa actggaaaac atttatatta atcataacaa tctggaaagt ttagatttaa
    11281 aaaatcagca ttcattggtt aatctggaag cgcaaaataa tagcctaaaa aaatgatatt
    11341 tctgatagtt ataaactgaa atttcttaat cttgattata acaagctagc atcgctggat
    11401 gtctcccggc aagaatctct gattgagtta agtgcccatc acaatatgat cactgacctt
    11461 atattacaca atcatcccag gatgaaaaca atcactttaa acgacaacca tattgcacat
    11521 ttaaacgcca aaaacactac aaaactggaa tatttaaact taagcaataa caatttactg
    11581 ccaaccaatg acattgatca actaatatta tcaaaacatc tttggcatgt attagttaac
    11641 ggcatcaaca atgatccact tgcacaaatg cagtactgga ctgcagtaag aaatataatt
    11701 gatgacacta atgaagtgac cattgattta tcaggactta atttaaccac tcaaccacca
    11761 gggctgcaaa acttcacctc tatcaatctt gataataacc aactcacaca ttttgatgca
    11821 accaactacg atagactcgt aaagctaagt ctgaatagta atgctcttga gtcaataaat
    11881 tttcctcaag gcagaaatgt aagtattaca catatatcta tgaataataa tgctctcaga
    11941 aatattgata tagataggct ttcatcagtt acttatttta gtgcggcaca taatcaacta
    12001 gagtttgtgc aattagaatc ttgcgaatgg ctgcaatacc tgaatctcag ccataatcaa
    12061 ttaactgata ttgttgcagg taataaaaat gaactcttac tgctggatct atcccataat
    12121 aaactaacaa gtttacacaa tgtcttattt cccaacttga atacgttact tattaacaac
    12181 aatttgcttt ctgaaattaa aatattctat agcaacttcc gcaatgttca gacattaaac
    12241 gctgctaaca accagttgaa atatataaat cttgatttcc tgacttatct tccatctatc
    12301 aaaagtttaa gactggacaa taataaaata acccacattg atactaataa tacatccgat
    12361 attggaactt tattccccat aataaaacag agcaaaaact taaatttttt aaatgtttct
    12421 gggaagaaca attgacctac tatgcagctc atgttattta atttgttttc cccagcactt
    12481 aagcttaata ctggcctggc aattctttcg cctggtgcat ttgaagttca ctctgacgga
    12541 atagatgcgg ataacgaatt gtttcactat actattaaaa aagcatatac cccatataat
    12601 atacacactt acaagacaga ggaagttgta aaccagagga atataaaagt taaa
//
LOCUS       DAJTRD010000099        12652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTRD010000099 DAJTRD010000000
VERSION     DAJTRD010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.099"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..1040
                     /locus_tag="OO315_003250"
     CDS             <1..1040
                     /locus_tag="OO315_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5892051.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1041..3206
                     /locus_tag="OO315_003251"
     CDS             1041..3206
                     /locus_tag="OO315_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5892052.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKEADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3220..4131
                     /locus_tag="OO315_003252"
     CDS             3220..4131
                     /locus_tag="OO315_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5892053.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4131..5426
                     /locus_tag="OO315_003253"
     CDS             4131..5426
                     /locus_tag="OO315_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5892054.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5471..5701
                     /locus_tag="OO315_003254"
     CDS             5471..5701
                     /locus_tag="OO315_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892055.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5679..6179
                     /locus_tag="OO315_003255"
     CDS             5679..6179
                     /locus_tag="OO315_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5892056.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6179..7597
                     /locus_tag="OO315_003256"
     CDS             6179..7597
                     /locus_tag="OO315_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5892057.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7597..8445
                     /locus_tag="OO315_003257"
     CDS             7597..8445
                     /locus_tag="OO315_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892058.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8445..8900
                     /locus_tag="OO315_003258"
     CDS             8445..8900
                     /locus_tag="OO315_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5892059.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8903..9595
                     /locus_tag="OO315_003259"
     CDS             8903..9595
                     /locus_tag="OO315_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5892060.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9605..10936
                     /locus_tag="OO315_003260"
     CDS             9605..10936
                     /locus_tag="OO315_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5892061.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10937..>12652
                     /locus_tag="OO315_003261"
     CDS             10937..>12652
                     /locus_tag="OO315_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5892062.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKAMPAGAKIDAPSTRSMLKQVAEDLGGMKNL
                     DPIEKR"
BASE COUNT         3406 a         3129 c         3335 g         2782 t
ORIGIN      
        1 ctttctttcc gaaccttgtt gatcaccttc tggttaagca tcacacggct gatggcgttg
       61 aagatggcat ttcaatctgc tacttcaagc catactcgca aggccgtgct cgctggcagg
      121 gtgacacaat ccacggcgtg tggtttgacg aagagccacc atacagcatt tatggcgaag
      181 gtcttacccg taccaacaaa tacgggcaat tctcaattct gacgtttacc ccgctgatgg
      241 ggatgtctga cgttgttacc aagttcctga agaaccccag caagtcgcag aaagtggtca
      301 acatgaccat ctatgacgct gagcactata ccgacgagca gaaagagcaa atcatcgcat
      361 catatcccga gcatgagaga gaggcgcgtg ctcgcggtat tcctacgatg ggtagtggtc
      421 gaatcttcca gataccggaa gagacgatta agtgtcagcc gttcgagtgt cctgatcact
      481 tctacgtaat tggcgggatg gatttcggat gggatcaccc acaggcgcaa gttcagcttt
      541 ggtgggataa ggaggcagac acaatctacg tttcacgcgt gtggaaggcg aaagaaaaaa
      601 cagccgttca ggcgtgggga gccgttaaat catgggcgca taaagtgcca accgcatggc
      661 ctcatgatgg aaaccagcac gagaagggcg gcggtgagca gctcaaaggg cagtatgcag
      721 acgctggatt tatgatgttg caggagcatg cgacatggcc tgatggcggt aacgctgttg
      781 agccaggcat cactgaattg cgcgacatga tgctcgatgg tcgcttcaaa gtattcaata
      841 cctgtgagcc attctttgag gagttccgcc tctatcaccg tgatgaaaac gggaaaatcg
      901 tcaagcttaa cgacgacgtg ctatccgccg ttcgctatgc atacatgatg cgccgcttcg
      961 caaaaatgat gcgcgacatc aaaaaaccaa aagagaaaaa gataccagcc ccaatcaggc
     1021 ccatcgcacg gagaacttaa atggccgacg aaaacagact caattccatt ctgtgtaagt
     1081 ttgacgcaga ctggatggcg agcgatgaag ccagaaccga ggcgacaaat gacctgtatt
     1141 ttagccgagt gtcgcaatgg gatgactggc tatcaaacta cactaccctg caatatcgcg
     1201 gacaattcga tgttgttcgc ccggaggtca ggaaactggt cgcagagatg cgccggaacc
     1261 ctgtcgacgt tctcttccga cccaaagacg gcgctaatcc tgatgcagcc gatgtattga
     1321 tggggatgta tcgtactgat atgcgccata acacggcaaa gattgccgtt aacgttggcg
     1381 ttcgtgagca gatagagtca ggcgttggtg catggcgtct ggtcacgcag tacgaagaca
     1441 acgatccaac aagcaacaat caggtaatcc gacgcctgcc aatccatgaa gcctgctcac
     1501 acgtcatatg ggacgccaac agcaagcaga tggataagag cgacgctaag cactgcacgg
     1561 tgattaacgc cttgtcgcgc aatggctgga aagagttcgc agaggattac ggtattgacc
     1621 cggataccct gccatctttc cagaatccta acgatacatg gctattcccg tgggtgtcga
     1681 atgatgtcgt ctacgtcgct gagtattacg aggtagaaga ggagaaggag aaggtcttca
     1741 tctaccgcga cccgctgaca ggtgagccgg tcagctatta ccagcaggat atcaaagacg
     1801 tcatcgacga cctggctaat cgtggattca ttaaggtagc agagcgcaag gtgaagcgtc
     1861 ggcgtgtgta taagtcgatc atcacctgta cgcagatact gaaagaccgc gagaagatag
     1921 ccggagagca tattccaatc gttccagtgt atggcgaatg gtcattcgct ggtgacaagg
     1981 agtgctacga gggtgtggta aggctgacga aagacggtca acgccttcgt aacatgatta
     2041 tgtcgttcaa cgccgatatt gttgctcggt caccgaagaa gaaaccgacc ttcttccctg
     2101 agcaaatcga aggctacgaa tacatgtacg gcggaaatga tgactatccg tactatctgc
     2161 agaacaggac tgatgaaaac ggtaacgacc tgccgattgg tccaatctcc tacatggaaa
     2221 accctgaagt gccgcaagcc aacgcttaca tgcttgaggc tgccaccaac gcagtgaaag
     2281 aagtggctag tcttggcgtg gatgcgcagg cggcaaatgg tcaggtcgct tttgataccg
     2341 tcaatcaact gaacatgcgg gcagaccttg agacatacgt gtttcaggat aacctggcta
     2401 ccgcaatgcg acgtgatggc gagatttatg cctcaatggt caacgatatt tatgacgttc
     2461 ctcgtcatgt aacgctgaca cttgaagatg gaagcgagaa agacgttcaa ctctatgcgc
     2521 aagttgtcga ttaccagtcc ggcaatgtgg tcacactcaa cgacattcgc ggtcgatatg
     2581 agtgctatac agacgttggg ccatccttcc agagtatgaa ggaacagaac cgcgcagaga
     2641 ttcaggagtt gctcaccaag gttccgcaag gtactccaga gttccagatg ctgatgctgc
     2701 aatacttcac gctgcttgac ggtaaaggcg tcgagatgat gcgagagtac gcgaacaagc
     2761 aactggtgat gatggggctg aagaaaccag aaacacctga agagatggag atggtgcagc
     2821 aggcacaaca acagccgcag cagccatcag cagagcaaat tcaggcgcag ggtatccttc
     2881 tgcaaggtca ggctgaattg ctcaaggcag agaaccaaca ggcgcagatt caggttgaag
     2941 ctgccaaggt tgaagcccaa aaccaactca acgccgcgaa gattgcagaa atattcaaca
     3001 atatggacct cgacaagcag gcagaactgc gtgagtacct caggctcgta ggtcaattcc
     3061 agcaacagcg cagcaaagat gctcgtgcta acgctgagct gcttcttaaa gaagcagacc
     3121 agactcattc acaacgcatg gatttcgcga atcttatgcg tcaagttcaa atcccctccg
     3181 gcggagtagc cgagacacct caataagaga gagttaatca tggaccaaac caccgacatt
     3241 caggcttctg aagaattaac cctgcccggc aatcatgcag cggcatctgc tgatggctta
     3301 gttgtcgata atgccaacga cagcgcaggt caggaagaag gctttgagat tgtccggaaa
     3361 gacgatgaga aaccaaaaca agacccggca actaatgctg aatttgcccg tcgccgcatc
     3421 gaacgcaaac gccagcgtga gcttgagcag cagatggaag cggttaagcg tggagagttg
     3481 ccggagcacc tgcgggtgaa ccctgagtta ccaaaacaac cagaccctaa cgattatctt
     3541 tccgaagatg cactggctaa gtacgactat gaccagagcc gcgcactggc tgccttccag
     3601 caggcaaaca gtgaatggca gatcaaggct atggacgcac gaagccaggc tgtcgccgag
     3661 cagggtcgca aaactcagga gttcacccag caatcagcgc aatacgtcga ggcggcccgt
     3721 aagcactacg aggcagcgga aaagctcaat atccctgact atcaggagaa agaggatgca
     3781 ttcatgcaac tggtgccgcc agcagtcggt gccgacatca tgcgcctctt cccggagaaa
     3841 tccgccgctc tcatgtatca ccttggtgct aatcctgaga aaacacgcca gttgctggcg
     3901 atggacgggc aatccgcgct gattgaactc actcgactgt cagaacgttt aactctcaag
     3961 cctcgagcca agcctgtttc agaagccccg ttacctgatg aacccattca gggacatgct
     4021 gttgctgcaa atatctctgc gattgaaaag cagatggagg cggcagcaaa caaaggggat
     4081 gtagagacgt accgcaagct caaggcgcaa ctgaataaag gaattcgata atggcattaa
     4141 atgaaggtca actggtcacg tatgctctgg atgaaatcat cggaaccgtc cagaacctga
     4201 cgccaatggc gtcaaaagtg acaaaatacc cccctccggc agaatccatg cagcgttcaa
     4261 gcaacaccgt gtggatgcct gttgagcagg aagcgccaac ccagactggc tgggatttaa
     4321 ctggcaacgc aaccgggatt ctggaactct ccgtgaagtg caacatgggc gatccggata
     4381 acgatttctt cgagcttcgt gcagatgacc tgcgtgatga gcgttcttac cgtcgccgca
     4441 tccaggcatc cgccaaaaaa ctggcgaata acattgagtc agcgattgcc aaacaggcaa
     4501 ctgaaatggg ctcgcttgtt gttcacgata cccgcgcaat tggtccatcg actggcctgc
     4561 ctggctggga ttttgtgtct gatgcagagc gactgatgtt ctcccgtgag ctaaaccgcg
     4621 acatgggcat cagttacttc ctgaaccctg acgattaccg caaagcaggc cgcaacctgg
     4681 tagatggtga catcttcggg cgcgttcctg aagaagcata tcgaaacggt actattcagc
     4741 gtcagattgc tggctttgat gaaattcttc gctcaccgaa acttccggca gttaccaagt
     4801 caaccgcgac tggtgtaact gtttctggtg cgcagaagtt taagccgcag gcatacaccc
     4861 ttgataccga tggtaacaaa gagaacgtcg acaaccgtgt tgcaacggtg accgtatcct
     4921 ccaccaccgg atttaagcgc ggcgacaaaa tcagcttcat tggtgtgaag ttcctgtctc
     4981 agatggcgaa gaatgtgctg actgatgacg cgactttctc aatcacccgt gtgatcgatg
     5041 gtactcacat cgaaatcacg ccgaagccga ttgcgctgga tgacgcgtca ctgacaaaag
     5101 aagagaaggc ttacgctaac gtaaacacct ctcttgctga taccactccg gtaaacgttc
     5161 tgaacgtggc aacaaccacc gctaacgtgt tctgggctga tgactcaatc cgcctgctgt
     5221 ctcagccgat cccggtaacc catgaactgt ttgctggcat gaaaacgtct tccttcagca
     5281 ttccaggtat tggtgttaac ggcatcttcg caacgcaggg tgatatcaac actctgtctg
     5341 gtaagtgccg tattgctgtg tggtattcag catgtgctgt acgaccagag gcaattggtg
     5401 ttggtctgcc taaccagacc gcgtgataac cagagggagc ttcggctccc gtttttatct
     5461 ggagacaagc atgacacaca tgatctttcg tcatggcgac atgaagaagt ggaaaggcgt
     5521 tggctacgac tttgaaatcg tgaaagccga agagcttcag gaatatctgg atgctggctg
     5581 gttttcacat cctgatgacc ttttgaagga tgttgcagag ccagagccag aagaaaaaca
     5641 gcgtaaaaag cctggtcgaa aacctaaggc ggcagcagat gaacctgaca acgaagggtg
     5701 atttagttct tgcggcatta cgtaagctcg gtgttgcatc aaatgccacg ttaaccgatg
     5761 tcgaaccgca gtctatggaa gacggcgtca acgaccttga aatgatgatg gctgaatggc
     5821 ttggcggtga tgcgtcacct ggtatcaacg ttggctacat ttttgctgat gcagatgttg
     5881 ctccggatcc gggcgatgag cacggtttat caaataacgc tatcaatgcc gtcattttca
     5941 accttgcctg ccgaattgct ccagattatg cgctggaagc gtctgcaaaa cttataacca
     6001 ctgccagata cgggaaagag cgactcgtca aactgtctgc aatggacagg gcaaaagcag
     6061 ctaaatgtaa gtccggttat ccaaaccgta tgcctgttgg tagtggtaac cagttggcga
     6121 agtggaatgg ttggaattac ttccaccgaa aggaaccttg cgataacggg agcgaataat
     6181 gcctattcag caacttccgc ttatgaaagg tgtcggcaaa gacttccgaa acgccgacta
     6241 tatcgactat ctgccagtga atatgctggc tacacccaaa gaaatcctga acagcagcgg
     6301 atatcttcgc tcattcccgg gcattgccaa acgttctgac gtgaacggtg tatctcgcgg
     6361 cgtcgagtac aacatggcgc agaatgctgt ttatcgcgtg tgtggcggca agctgtacaa
     6421 aggagaaagt gaagtcggtg atgttgccgg aagtggtcgt gtatcaatgg cgcatggtcg
     6481 gacatcacag gcggtaggcg ttaatggtca actggtcgag tatcgctatg atggcacggt
     6541 caaaaccgtc tcaaactggc ctacagacag tggattcact cagtatgagt taggttcagt
     6601 tcgcgacatt acgcgcttac gtgggcgtta tgcgtggtca aaagacggaa ctgattcatg
     6661 gtttatcact gaccttgaag acgaatctca tcctgaccgt tacagcgcac aatatcgtgc
     6721 cgagtctcag cctgacggca tcatcggtat cgggacatgg cgagacttca tcgtctgctt
     6781 tggttcatcg actattgaat atttctccct gactggcgca accaccgttg gtgccgcttt
     6841 gtatgttgca caaccatcgc tgatggtgca gaaaggcatt gccggaactt actgcaaaac
     6901 gccattcgct gattcgtatg cgtttatcag caatccggca acgggtgcgc cgtctgtgta
     6961 tatcatcgac tccggtcagg tgtcaccaat tgccagcgcg agcattgaga aaatcctccg
     7021 ctcctacact gctgatgaac tggctgatgg tgtgatggaa tcgctgcgat ttgatgctca
     7081 tgagttgctg attatccacc ttccgcgtca cgtcctcgtg tacgacgcat cttcaatcgc
     7141 caatggccca caatggtgtg tgctgaaaac aggtctgtat gacgatgtgt accgcgctat
     7201 cgacttcgtt tacgaaggca atcagataac gtgcggcgat aagctggaat ctgttaccgg
     7261 gaaactgcaa ttcgatatca gcagccagta cgacaagcaa caggaacacc tgctgtttac
     7321 tccactcttc aaagcggata acgccagagt gttcgacctt gaggttgaat cgtcaactgg
     7381 cgttgcgcag tacgccgacc gcctttttct ctctgcaacc actgactgca tcaattacgg
     7441 gcgtgagcag atgattgagc agaatgaacc gttcgtttac gacaagcgtg ttttgtggaa
     7501 gcgagtaggg cgcatcagga aaaatgtcgg cttcaaattg cgcgttatca cgaagtcacc
     7561 tgtcactctg tctggcgctc agataaggat tgagtaatgg ctgattcgaa tctcaatgtg
     7621 ccggtaatca tccaagctac gcggctcgat acatcagtcc ttccacgcaa tatcttctcg
     7681 cagtcgtatc tgctttacgt tatcgcacag ggcactgatg ttggtaacgt ggctaacaaa
     7741 gccaacgagg ccggacaggg cgcttatgat gcacaggtca ggaacgatga gcaggatgtg
     7801 attctcgctg accatgagca gcgaatttct gctgcggaag caacgcttgt taatcatgag
     7861 gagcgaatca gacaggcaga atcaactctt caggaccatg aaacacgaat agctcagaat
     7921 gaaagcgata tttcgtcgct tgacacaaga gttcagtcgc tggagtcgca ggtttcagac
     7981 catgaaacgc gcatcgatgc tctggagtat gcaaccacac gcaagaagtc agaggttgtt
     8041 tactctggtg tatctgtaac catcccgaca gcgccgacca accttgttag cctgctgaaa
     8101 acgctcacac cgtcatccgg ctcgttggca ccattcttcg acaccgttaa caacaagatg
     8161 gttgtgttca acgagaacaa aaccctgctc ttcaagttgt cgattgtcgg cacgtggccc
     8221 agcggaaccg ccaacaggtc aatgcagcta accttttccg gctctgttcc tgacacactg
     8281 gtcagcagtc gtaatgcggc gacaacaacc gacaacatcc tgttagctac gttcttcagc
     8341 gtggataaag acggctttct tgccacaaat ggcagtacgt taaccattca gtcgaatggt
     8401 gcggcgttta ctgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac
     8461 caacgcgaaa catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga
     8521 gcaacaacgg tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc
     8581 acggtcagtt tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc
     8641 acgccatgta cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc
     8701 gatacattct gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc
     8761 gacacggtca gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat
     8821 acttcaaagg cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact
     8881 tcctgaatca cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgcgcgg
     8941 cgaggaacct tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa
     9001 gtatgcagca gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat
     9061 catgaaaaac cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa
     9121 cctttccagt ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta
     9181 taaagacctt gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg
     9241 acttggttcg acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc
     9301 ttggttatca aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct
     9361 gcaaggtcag gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca
     9421 aagcgcagct cttgccgctg ctaatgccaa taaaccatca agtcttcaga ctgcaattag
     9481 cggtggcacg tctggtgcga ttgccggtgc aggtcttgcc agccttttgg gaacatcaac
     9541 gccttggggc gctggtattg gtgctggtat cggattgctt ggctcgttgt tttaaggggg
     9601 aatcatggct acttggcaag gaacaaacgg cggattgttg gctggtatcg gcggcgtcaa
     9661 ctcaaacgct ccgagcgtaa atgacatcgg caatacgctt cagcttatca ggcagaacaa
     9721 tgatattgag cgttcaggcg ctaacaatgt tgggctgact gctttgcaag gtctttcagg
     9781 tattgcgggt gtttttcagc aggaaaagca ggctcagcgg cagaaagaat ttcagcaggc
     9841 gtacgctaat gcttatgcgt ctggtgatcg cggtgctttg cgtcagttgg ctactcaata
     9901 tccagaccag attgaatccg ttcgtaaagg catgggattc attgatgaag agcagcgtaa
     9961 ttctatcggc accttagcgg ctggcgcacg ccttgcgtca tcgtctccag aagcaatgca
    10021 atcatggctg caaaacaacg ccggtgagtt agctcgtgtt ggcgttaatc ctcatgacgt
    10081 cgctcagatg taccaacaga acccgcggca gttcggcgaa tttgtcgatc acctggggat
    10141 gaacagtctc gggcccgaaa aatactttga cctacaggat aaaatgcagg gtcgccaggt
    10201 taccatgcgc ggtcaggatc tggattcgca aaccgccgct cggaatcagg caatcacaat
    10261 gcgcggacaa gatatccagg cgaatttagg tcagcagcgc attaatctgg acgcagaaac
    10321 aaaccgcatt aacaacgaaa ataagcgcct tgaccggatg ctatcagcag aaactaacga
    10381 cctgaagcgc caggaaatac agagccgcat agcagccaac aaccagcagt tgcagcagaa
    10441 gcagcaagcg ctaaatgatg gctacaaaga cggcatcaac accctcacaa ccagcatgtt
    10501 cactctgaac gatatcgtta gttctccttc acttaagagc attacaggct tacgtggagt
    10561 aatccccaac gttccaggct cacaggctgc agacactcag gcacgacttg atacctttaa
    10621 atcacaagca tacctgacag cggttcaggc catgcgaggc atgggcgcac tttctgatgc
    10681 cgagggcaaa aagctcgacc aggctgttgg ttcgctgcag aactcgcaga gcgaggagtc
    10741 ctttcgtcgc aacgctggcg tcatcctgaa cacgctcaac cagaagcgta atgaggcggt
    10801 tggtaagtac gttcagcaaa acggtatcaa gcgagtggaa gcgcctcagg cttctataga
    10861 ttacctgaag cagcaccccg agctgtcaat cgacttcatt aatcgctacg gatatcttcc
    10921 atctttgggg cagtaaatgg ctaattaccg tgatttgtta gagcaggctg gcgcacgtta
    10981 cggtgtgcca gaagggttga tgactgcact gggtgccaag gagtcttctt acaaccctgc
    11041 cgcagtaagc tccgccgggg ctgtaggatt gactcaggtc atgcctggga catggcgtga
    11101 tatgggttat accgatgagc aaatgcaaaa ccccgaatat caggctgacg ctggcgcgcg
    11161 ctatctggca aagatgtacc agcagtttgg taactggcgt gactctcttc aggcttatca
    11221 cgacggtccc ggcaacgtta tgaaggcaaa gcgtggtgaa tatacgccag gacctgaagg
    11281 ccgcggttac gttgatgatc gctttgctca atgggctggt gacccggtga cagactcaac
    11341 agtcgaacag cgcgccacct ctgcaaaggt acatcctcag caagacccta acaacccgtt
    11401 tgcacaactg gaagtacagt catccgaaca agtatcggca tcaggtgtgc agtcagaccc
    11461 aaataatcca tttgctcaga ttgagcagca ggcagccagt cagcagccac ctcaacccgt
    11521 aagttctgtc gcaccgaaac ctgttcagca acaaacagtt aatcaggcca ataatgcacc
    11581 aacacgggaa gaaccatcat tgatgcaaca agctggcgat tggctcactg gtggtcaaag
    11641 tgcagggcaa attgcagaac aggctggtcg tggtctggta aacataccat ttgacgtatt
    11701 gcagggtggc gcaagtctga ttaatgcaat cagtcagggg cttggggggc caaaagtatt
    11761 ggatgatgtt tatcgtccag tagacagacc gacagacccc tacgcgcaag ctggagagtc
    11821 aataggcggt tatcttgttc ctggagcagg tgtagctgga aacatggtca ttggttctct
    11881 cgctgacgcg gcgaatcaac ggggtgattt tgccgaaaat gccgctatta atgccggact
    11941 tagcattgct acgcatggcc tgataaatgg cgttacccgt ggtgttcgtg gtgcatcaaa
    12001 tataattagt ggcaataaaa catctgcaca gagagcgacc actgcgccaa cagaaacatc
    12061 accattctcc ggtgatgccg ctgcagcaac aaatcctgcg gttcatgccg cagaggcaag
    12121 agtagcacaa ggtgtaccaa tgacgcctgc gacgaagaac ccagaggaag tcgttcgcac
    12181 agtagcagca caaaaaaggc caaatctcgc ttcatcgctt gatgaactag atatcaatcc
    12241 tcaggctgaa gttctggagt ctgctgaaag gcttaatgtt gattcattac tcccttcaca
    12301 cttttccggg aacgagcaat acaaggcagt tgagcaagca atcaagtccc gtgcgggttc
    12361 tgctctacag gtgcaggaaa atgaagcaat caggcagcta gcacagggcg cgggtgagat
    12421 aattgatcgc gtttccggtg caaaagatgc tcttggtatg agcgacaagt ttattgatac
    12481 ggtcaatgga agaatgtctg cgctgatgaa acgaagcgac cagctttatc gcaatgttga
    12541 aaaggcgatg cctgcaggtg caaaaattga tgcgccatca acaaggtcaa tgctcaaaca
    12601 ggtggcagaa gatcttggcg ggatgaaaaa ccttgaccct attgaaaaga ga
//
LOCUS       DAJTRD010000100        12624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTRD010000100 DAJTRD010000000
VERSION     DAJTRD010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.100"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            758..841
                     /locus_tag="OO315_003262"
     CDS             758..841
                     /locus_tag="OO315_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5892063.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO315_003263"
     CDS             complement(895..2247)
                     /gene="gorA"
                     /locus_tag="OO315_003263"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5892064.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO315_003264"
     CDS             complement(2319..3161)
                     /gene="rlmJ"
                     /locus_tag="OO315_003264"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5892065.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3364..5406
                     /gene="prlC"
                     /locus_tag="OO315_003265"
     CDS             3364..5406
                     /gene="prlC"
                     /locus_tag="OO315_003265"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5892066.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO315_003266"
     CDS             5414..6166
                     /gene="rsmJ"
                     /locus_tag="OO315_003266"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5892067.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO315_003267"
                     /pseudo
     CDS             complement(6215..7684)
                     /gene="dtpB"
                     /locus_tag="OO315_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO315_003268"
     CDS             complement(8002..8436)
                     /gene="uspA"
                     /locus_tag="OO315_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5892068.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8830..9165
                     /gene="uspB"
                     /locus_tag="OO315_003269"
     CDS             8830..9165
                     /gene="uspB"
                     /locus_tag="OO315_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5892069.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO315_003270"
     CDS             complement(9236..10735)
                     /gene="pitA"
                     /locus_tag="OO315_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5892070.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
     gene            10967..12166
                     /locus_tag="OO315_003271"
     CDS             10967..12166
                     /locus_tag="OO315_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5892071.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLASIVK"
BASE COUNT         3106 a         3168 c         3356 g         2994 t
ORIGIN      
        1 taacagaaat gacatattgc gctcctgatt gttgcaggta gtgtctctct tcgaagcgga
       61 taagtcaaaa acatatatga cttaacgaat gtgtaagtgc agaggaaggt aataggtgtg
      121 aattttgagt tggctattca tttgaaaggc ggtttgaatc aggacgtaaa aaaaccgact
      181 ttgcgtcggt tttttactct tcagcctgag ttggtggctc tggctggagt gagaatgccc
      241 acatctaacg gttcaatact attagccagt ggctaacatt gcaagcgtta gcgatttctt
      301 cagctggaga agggacagtt tgctatacat ggtggtgaaa gaggatttat atactttcat
      361 cgggtcgctg ccgtaccaag ccttgtccga atccggacgt aaaaaaaccg acttcgcgtc
      421 ggttttttta ctcttcagcc cagagttggt ggctctggct ggagtgagaa tgccatcatc
      481 taacgcatca atgctaatac catactgaaa ctattgcaag gttatgctgg ttatttagcc
      541 taattacact gtatgattat ccagttagct ctgaggcatt ttcactctgg caatgcgcat
      601 aaacgctttc aaagtcctgg tcagaagtac gggtggtgcc gttaactgat gctctggccg
      661 gagtgagatt gcccacatct aacaatgagg catgcgccgt gacaggcagt ggatgagtaa
      721 gcggatgcat tctcactcca tcgcatggag aaaacgggtg attgataaag caatcatcgt
      781 tcttggggcg ttaattgcgc tgctggaact gatccgcttt ctgcttcagc ttctgaactg
      841 atagcggaaa cgtaattaag ggctaagagc acactactct tagcccttta acatttaacg
      901 catggtcacg aactcttctg ccgccgttgg gtgaatggcg acggtattgt cgaagtcttt
      961 tttggttgcg cccatcttca gcgccaccgc gaagccctgc aacatttcgt ccataccaaa
     1021 gccaatgccg tgaataccga caatcttctc ttctggccca acgcacacca gcttcatgcg
     1081 gcacggctgg cggtgagtgg tgacggcggt atacatcgcg gtgaaagagg atttatacac
     1141 tttcacctga tcgtcgccgt actgctcgcg cgcctgcggt tccgttaaac caacagtacc
     1201 aatcggcgga tggctgaaga ccacggtcgg aatgttgctg taatccagat gctcatccgg
     1261 cttgttatta aacaggcgtt cagagagacg gcgacccgct gcaactgcca ccggtgtcag
     1321 ttccactgca cccgtgttat cgcccaccgc gtaaatacct tcaatattgg tgttttgata
     1381 tttatcgacg acgatatagc ctttttcgtt ggttttaacg ccagcggctt cgaggttgat
     1441 gttgtcattg gcaggctcgc gaccaatcgc ccaaatcagg caatccaccg tttcactgcg
     1501 accatcttcc agctccagcg tcaggctacc atcggcattt ttcactaccg ctttcgggat
     1561 ggcgttggta tgcaactgtg ggccttcggc gttcatcact tcgaccagcg tttcagagat
     1621 catcgggtcg aagctgcgca gcggcgcatg tttacgcaca aacagatgcg ttttcgcgcc
     1681 gaggccgtta atcacgccag ccagctcaac ggcgatgtaa cccgcgccaa caaccgccac
     1741 gcgctctggc aaagcaggaa gggcgaagaa gccatcggaa tcaataccgt attccacgcc
     1801 tggaatatcc gggtggctcg gacgaccgcc ggtggcgatc agaatatgat cggccgtgat
     1861 ggtttcgccg ttaacttcca gcgttttggc atcaacgaag cgggcaaagc ctttgattac
     1921 atcaacgtta tttttaccga gcacgttttc ataggaagta tgaatacggt cgatataggc
     1981 ggtacggctg gcgatcaacg tttcccagtt gaacttgttg atggtggtat cgaaaccata
     2041 atcagggccg tacatatgga tcgcttcacg gatttgcgcc gcgtgccaca tcactttttt
     2101 cggcacgcag ccgacgttca cgcaggtgcc gcccagctct ttggcttcaa tcagtgcgca
     2161 tttctggccg tacatagccg cgcggttgat ggaggcgata ccgccgctgc cgccgccgat
     2221 ggcgatgtaa tcatagtgtt tagtcatgac atattgtcct tatcgttgat taccgcgatt
     2281 gtagcgcgag acgtaatagg tgccagcaat ggctgcaatt actccggcac gatccagctt
     2341 acggtggcgt gcccggtacc tgccggaacc agtttgctgt gcagccacga cagcacgtta
     2401 ttcatctgct gttccagctt ccacggcggg ttaatcacaa tcatgccgga agcggtcatg
     2461 ccacggcgat cgctgtctgg cagcaccgcc agttcaattt gcagaatttt gcgaatgccg
     2521 gtcgcttcca ggtcgtggat catgcgctta atttgctggc gcagcaccac cggataccac
     2581 aatgcgtaag tgccagtggc gaaacgtttg taaccttctg ctatcccgct gaccaccgcc
     2641 tgatagtcgg ttttcatttc atacggcggg tcgataagga ttaaaccacg gcgagaaacc
     2701 ggcggcagtt tggccttaag ttgctggaaa ccgtcggctt tttcgacgcg tgcacggctg
     2761 tctttctgaa atccagaacg cagcaacggg taatcgctcg ggtgcagttc ggtcagttgc
     2821 aggctgtctt gttcgcgcag tagctggcga gcaatcaacg gcgaaccggg gtagtaacgc
     2881 aactggccgc tacggttgaa gtgttttacc acattgatgt acgcctccag ttctgcgggc
     2941 aaatcgtcct gctgccagat acgggcgata ccttcgagat attcgccggt acgctcggca
     3001 tgttcgctac ctaactgata acgcccggcc cctgcgtggg tgtcgagata gagaaacggt
     3061 ttatctttct ctttcagcga ctcgatgatc aggctctgaa cggtatgttt aaggacgtcg
     3121 gcgtggttgc cagcgtgaaa gctgtggcga taactgagca tgggtaaagg tgttccggta
     3181 agtaaaaatt ggcccgtatt cggggcgcac aaaagcctat caggacagta taccgaaaag
     3241 aggccgccgc cgcgaaagcg taacgtttct cattgaaatt cactacactt aaccccatgc
     3301 tacacacatt atgtaaagcg cctgttgagc gcttccttaa cctctttaac caggactgcg
     3361 ctaatgacga atccgttact gactcccttt gaattgcctc cgttttctaa aattctcccg
     3421 gaacatgtcg ttccagccgt gactaaggca ttgaacgact gccgcgaaaa tgtggagcgc
     3481 gtagtagcgc aaggggcacc gtacacctgg gaaaatctct gccagccgtt ggcggaagtg
     3541 gacgatgttt tggggcgtat cttctccccg gtcagccacc tgaactcggt gaaaaatagc
     3601 ccggaactgc gtgaagcgta cgaacaaacc ctgccgctgc tgtcggaata cagcacctgg
     3661 gtagggcaac atgaagggct gtataaagcg taccgcgacc tgcgcgatgg cgatcattac
     3721 gccacgctga acacggcgca gaagaaagcg gttgataacg ccctgcgtga ttttgaactc
     3781 tctggcatag gtctgccgaa agagaaacag cagcgttacg gcgaaattgc tacccgtctt
     3841 tctgaactgg gcaaccagta cagcaacaac gtcctcgatg cgacgatggg ctggaccaaa
     3901 ctcgttaccg acgaagcggg gctggcgggg atgccagaaa gcgcgctggc tgcggcaaaa
     3961 gcccaggccg aagcgaaaga gctggaaggc tacctgctga cgctggatat cccaagctac
     4021 ctgccggtaa tgacctactg cgacaaccag gctctgcgcg aagagatgta tcgcgcttac
     4081 agcacccgcg cctccgatca aggcccgaat gccggtaagt gggataacag caaggtgatg
     4141 gaagagatcc tcgcgctgcg tcacgaactg gcgcaactgc tgggctttga aaactacgcc
     4201 tttaaatccc ttgccactaa aatggcagaa aacccgcagc aggtgctgga tttcttaacc
     4261 gatctggcaa aacgcgcgcg tccgcaaggc gaaaaagagc tggagcaact gcgtgccttc
     4321 gctaaagccg aatttggtgt cgatgagttg cagccgtggg atatcgctta ctacagcgaa
     4381 aaacagaaac agcacctcta cagcatcagc gacgagcagc tgcgtccgta cttcccggaa
     4441 aacaaagcgg ttaacggcct gtttgaagtg gtgaaacgta tttacggcat caccgctaaa
     4501 gagcgtaaag atgttgatgt ctggcatccg gatgtacgtt tcttcgaact gtatgacgaa
     4561 aataacgaac tgcgcggcag cttctacctc gatctgtatg cccgtgaaaa caagcgcggc
     4621 ggggcgtgga tggatggctg cgtaggccag atgcgtaaag ctgatggttc tctgcaaaaa
     4681 ccggtcgcgt atttgacttg taacttcaac cgcccggtaa atggtaaacc ggcgctgttc
     4741 actcacgacg aagtgatcac cctgttccac gagttcggtc acggcctgca ccatatgctg
     4801 acccgcatcg aaaccgctgg tgtttccggt atcagcggtg tgccgtggga tgcggtcgaa
     4861 ctgccgagtc agtttatgga aaactggtgc tgggagccgg aggcgctggc gtttatctct
     4921 ggtcactatg aaaccggcga accgctgccg aaagagttgc tggataaaat gctggcggcg
     4981 aagaactacc aggcggcgct gtttattctg cgtcagctgg agttcggcct gtttgatttc
     5041 cgccttcatg ccgagttccg cccggatcag ggggcaaaaa tcctcgaaac tctggcagaa
     5101 atcaagaaac tggttgccgt ggtgccatct ccgtcctggg gccgtttccc gcacgctttc
     5161 agccatattt tcgccggtgg ttatgcggca ggttactaca gctacctgtg ggctgacgta
     5221 ctggcggcag atgctttctc gcgctttgag gaagagggca ttttcaaccg tgaaaccggg
     5281 cagtcgttcc tcgacaacat tctgagccgt ggcggttcag aagagccgat ggatctgttc
     5341 aaacgcttcc gtggtcgtga accgcagctg gatgcgatgc tggagcatta cggcattaag
     5401 ggctgatcat tcagtgaaaa tctgcttaat tgatgaaaca ggcgccggag acggcgcctt
     5461 atctgttctg gcggcccgct gggggctgga gcacgatgaa gacaacctga tggcgctggt
     5521 gttaacgccg gaacatctgg aattgcgcaa gcgtgatgag ccaaaacttg gcggcatctt
     5581 tgttgatttt gttggcggag cgatggcgca ccgacgcaaa ttcggcggtg gtcgcggtga
     5641 ggcggtggcg aaagcggtgg gcattaaagg cgattatttg ccggatgtgg tggacgccac
     5701 cgcagggctg gggcgcgatg cctttgtact ggcttcagtc ggctgccgcg tgcggatgct
     5761 ggagcgtaat ccagtggttg ccgcgctact cgacgacggc ctggcgcgtg gttatgcgga
     5821 tgcggaaatc ggcggctggt tgcaggagcg gttgcagtta attcacgcct ccagcctgac
     5881 ggcgctaacc gatattaccc cacgcccgca ggtggtctat ctcgatccga tgttcccgca
     5941 taagcagaaa agcgcgctgg tgaaaaaaga gatgcgtgtc tttcagtcgc tggtggggcc
     6001 ggatcttgat gccgatggat tactggagcc tgcacgcttg ctggcgacca aacgcgtggt
     6061 ggtaaagcgc ccggactacg cgccaccgct ggcgaatgtc gccacgccaa acgcggttgt
     6121 gactaaaggg catcgctttg atatctatgc aggtacgcca gtgtaaaagt aaagcagggt
     6181 agcgataacg ctaccctgct ttctgcaaga ataattaatg gctttccggc gtcgcaatca
     6241 tgcgtttcag ccacggcacc atcaacagca tcactactgc aacgcccagc gtgaccagac
     6301 caatcttacc aaacacgttg gtatagacgg gcaacgtctc aagcggatcg gtaatgttgt
     6361 ccggcaccgc ggtaaatgtt gccacatagc cgcccagcaa gaacgcggca gcctgcgtca
     6421 ggaaccacat cccgagaata aagcccatca aatgctgcgg caccagggca gcaatcatcg
     6481 ccaggccaag ggcgctaata aacaattcac ccaggctcta gaataaatac accagcacga
     6541 taaaccatgg cgatgtcagc ccctggtcat ccgcaaacca cattcccgca gcagcagccg
     6601 tcaaaaagcc cagtgagcac ataaacatgc tgagagtaaa tttcatcggc atcgagaggt
     6661 ctttgccttt gttacccaga tgcgtgtaaa tgcctgccag tattgggctg gcgagtacca
     6721 cccagaacgg gttaagcgcc tggaagctga ccgggttgat ggaaaaaccg agaatttcat
     6781 gatgcacgtt gttgatggca aagaagttca gcgatgttgg catctgggcg tagagaatgt
     6841 aaaacaccac cgcttcgagc atcaggacaa aggcgacaaa cattttattg cgcccggttt
     6901 tatccagctt gaatgcctga cgaaagaaga tgatagtgac gacgatggag agaacaatca
     6961 gcaccagatt ggcgacttct acgttgtgca tcagccatgc acatacgaag atcatcacca
     7021 cgctgccaag taatacgtac aacagtttgc tgaagctcat cggcttgaag tcgggttcag
     7081 aaccaatgtc tttcaccatt ccacgacagg cgatgtaaac cagtaatgcg ataattaacc
     7141 ccgccccgca caggttgtag gtgactgaat aaccgaatct atcagcgatc acaggggcca
     7201 gcgataacgc tatcaacgag ccgatattga tcgacatata gaacagggtg aatgcgccat
     7261 caagccgcgg atctttcggc ggatagcact tcgaaagcaa gctggctggg ttagctttaa
     7321 acaggccgtt accgacagcg atagtcccca gggcgatgaa aatcaggtca ggcttaagta
     7381 gcgacatgcc ggtcatgaag tagccaatcg ccagcacaag tgctccaaga acaatggtgc
     7441 gtttggtccc caacaggtgg tcgccgacat agccgccaat ggaaatgagg ccatagacca
     7501 gtgcagcaaa agcaccaaaa gtgacaaaag cctgctcttg cgagaatcca agctgtttaa
     7561 cgaagaaaac cgccagtacg ccctgcacgc cgtagtagcc gaatcgctcc cataactcga
     7621 caaaaaagat catgaaaaat gggcgaggtt gctgcagcat ccccatgggt gttgttgtat
     7681 taatatttat taaccttcca ataccatcct gaaaagtatg aacgcgtaag ggataacgcg
     7741 cgaaaaagtt tacatctgtc agagattctt aatattgatg cggtgaatag attaatacac
     7801 tattacaaca gaaaataacc agctgatcac actaatgatg gaaaatatga tggtgttaaa
     7861 ttttgtattg gcaggagatt ttgattgcgt acgcagatcg taggccagat aaggggttta
     7921 cgctgcatca ggtaacaagg cgcgccaaaa agcaaaacgc ccgcatatgc gggcgttcag
     7981 gaacacgtcg tggagggatg attattcttc ttcgtcgcgt agcggaacaa tcagcatatc
     8041 aacgtgaacg gtgttgatca gctgacgtgc ggaagacatc agtttgctcc agaagtcctg
     8101 gtggtgacca caaaccacca gatccatatc gtatttcttg attgcatcga ccagaacctg
     8161 gcctaggtcg ccgctgccgc tcagggtttc agtgattgga tagcctgcat tagtggaaag
     8221 ctcggtcagc gcatgatgtg tctcttcaga gatgcgtttc tgcatatcac ccagattcac
     8281 atcaataagc ccggtgtata ggtcagagta gtttacatct acgtggatca gagaaacttt
     8341 cgcattgtag gggcgagcca tagagactgc tttctctacc agaactttgc tttccgggga
     8401 gagatcgact gcgatgagaa tgtgtttata agccatagtg ttactccttc cataaagttg
     8461 tcgatgactg gccagctagc gtttcttgtg ccgttacgtc acccgcgtcc tgcgaactac
     8521 gtgcgcgggg ctaacccagc cgtaaagatt cagtgttaag accatcctta ccttatagcg
     8581 acccggatga tccgtcaatc cgccttgctt acccgttaga taaacaaatt ttcgacatga
     8641 atattatatt gatagtggtt aaccttctgg aaaaaaaaca acctgatctc ctacactatc
     8701 tatagagccg ctcggatgtt ggtcaccaca aaagcggttt ctccaggacc tggtgttaac
     8761 tgattggcag cgtatcggga gcggtagccc cggggtggaa ctccgcgggc aggtcgccgg
     8821 ggaggaggta tgataagcac cgtcgcatta ttttgggctt tatgtgtcgt ttgcattgtt
     8881 aacatggcgc gctatttctc atcactacgc gcgttgttag tggtactgcg taactgcgat
     8941 ccattgctct atcaatatgt tgatggaggg ggctttttta cctcacatgg ccaacccaac
     9001 aaacaggtgc gtctcgtttg gtatatctat gcccaacgtt atcgcgatca tcacgatgat
     9061 gagtttattc gccgctgtga gcgggtgcgt cggcagttta ttctgactag cgcattgtgt
     9121 ggtctggtgg tggtcagcct gattgcattg atgatttggc attaagcact tcagcaataa
     9181 taacgtataa aaaaagcggg ccagtaaact gacccgctcg ttttggtgcg tacgattaca
     9241 ggaactgcaa ggagaaccag tacagcccgc cggaaagtag taccgcagcc ggaagggtaa
     9301 acacccaggc catcagaata ctggttacgg ttttacgctg taagccgccg ccatctacca
     9361 ccatcgtccc cgcgacagaa gaggagagta cgtgagtggt ggaaaccggc atcccggtat
     9421 aactcgccag gccgattgac actgccgccg tcatctgggc agacatcccc tgagcgtagg
     9481 tcatgccttt cttaccgatt ttctcaccga tagtcgttgc cacacgacgc cagccaatca
     9541 tcgtaccgat acctaacgcc agcgcgaccg ccatgatgat ccacaccggc gcatactcga
     9601 tggtgctaag catgtcggac ttcagtttct tcaacaggcg ctgatcgtca gcactcacac
     9661 caggcatctt caccactttg tcgatagtgt cagaaacgca cagcataatg cggcgcatct
     9721 ggctacgttg atcaagcgac agcttgtcgt agctttccac atcggtggtc aacatacctt
     9781 tcaggcggtt gagcgcgtta atggtattcg acggatggca gtggaactcc gcaggttgcg
     9841 ttgcgccagc ttccggagcc ggtactaact gatcagcacc ggtagcctgt ttgagcagcg
     9901 caggatgctg ctcaaagtaa gcttcgacgt tgttgatggc atcacgggta cgggtgattt
     9961 cgtagccagt ggcattcatg ttcaccacga agcctgctgg cgcgacgcca atcaatacca
    10021 acataaccag accaatgcct ttctgaccat cgttcgcgcc gtgcgaaaac gccacgccga
    10081 tagcggaaag gatcagcgcg atacgcgtcc agaacggcgg ctttttcttg ccgtctttct
    10141 tttcacgctc cgctggggtc aggtggatac gggcgcgttt cttggtgccg ctccagtagc
    10201 gacgcagcaa gaaaatcaga ccgccagcaa acaccaggcc gacaataggg gaaacgatca
    10261 gagaaccgaa aatacttaat actttcggga tattgagtgc atccaccact gacgtcccgg
    10321 tcatcaacgc attggttaaa ccaatcccga tgatcgcgcc aatcagcgta tgagagctgg
    10381 atgcaggtaa accaaagtac caggtaccca ggttccagat aatcgccgcc agcaacatag
    10441 agaacaccat ggcaaggcca tgagacgatc ccatattaag cagcagatcc gtcggcagca
    10501 tatgcacaat ggcataggca acactcagac cacccagcaa aacacccaaa aagttgaaca
    10561 ccgccgccat aaccacggcg agctgagaac gcatcgcgcg ggtatagata acggttgcca
    10621 cggcgttggc tgtgtcatgg aaaccattga tggcttcgta gaacagcaca aaagccagtg
    10681 caagcaataa taacagcccg gtatgcaaat ccaggccagc aaacaaatgt agcataggac
    10741 gttacgccat tttgaggaca tgaacgcggc gcattatcag tgactttcgc ggcgcgggca
    10801 aagtgaaata tagacttttt ttgatttgcc tcctgtatgg atttcactca aaaaataatt
    10861 atcttatata attcaggcaa atacttcctt ttagtaatat tgatgctggt gcgaccactg
    10921 aggaatcttt acaattcacg cccgtttttt ctaagaggag cgcaacgtgg aaaggtttga
    10981 tgccattatt ataggcgctg gtgcggcggg tatgttctgt tctgcgctgg caggtcaggt
    11041 aggacgccgg gttctgctga tcgataatgg taaaatacta gggcgcaaaa tccttatgtc
    11101 tggcggtggg cgctgcaact ttaccaacct ttatgtcgaa ccaggcgctt atctgagcca
    11161 gaatccgcat ttttgtaagt ctgcactcgc acgttttacc cagtgggatt tcattgatct
    11221 ggtcaataaa cacggtatcg cctggcacga gaaaacgtta gggcaactct tctgcgatga
    11281 ctccgcgcag cagattgtcg acatgctggt ggatgagtgc gagaagggca atgtaacctt
    11341 cagattgcgt agcgaagtgc tgagtgtggc gaaggatgaa acaggcttca cgcttgatct
    11401 gaacggcatg actgtcggtt gcgaaaagct ggtcatcgcg accggtgggc tgtcaatgcc
    11461 ggggctgggc gcatcgccgt ttggttataa gattgccgaa caatttggcc tcaacgtgct
    11521 gccgacccgc gcgggtctgg tgccattcac tctgcataaa ccgttgctcg aagagttaca
    11581 ggtgctggcg ggcgtggcgg tgccttccgt gattaccgct gaaaacggca ccgttttccg
    11641 tgagaactta ctcttcaccc accgcggctt atctggaccg gcggtgttgc agatttcaag
    11701 ctactggcaa ccgggtgaat ttgtcagtat caatctgcta ccggatgtgg acctcgaaac
    11761 cttcctgaat gagcagcgta acgcacatcc gaatcaaagc ctgaaaaaca cactggcggt
    11821 tcatctaccg aagcggttgg ttgaacgctt acagcaactc gggcaaatcc cggatgtttc
    11881 gctaaaacag ctcaacgtgt gtgaccaaca ggcactgatt agcacattga ccgactggcg
    11941 cgtacaaccc aacggcactg aaggctatcg cactgccgaa gtgacgctcg gcggcgtgga
    12001 caccaacgaa ctttcttcac ggacgatgga agcgcgcaaa gtgcctgggc tgtacttcat
    12061 cggcgaagtg atggacgtta ccggctggct ggggggctat aacttccagt gggcgtggtc
    12121 gagtgcatgg gcttgtgcgc aggatttggc gtcaattgtg aaataaccat gacgttttcc
    12181 cgtcagccgt ttgtatatgt cgttattaag tcattttggt acggttctgc atccgacatt
    12241 ctggtgttaa ctcgctgttt tgttagcgaa ttaacgctat tgccgttgtg gttattttat
    12301 agtgtattta ttctcagaga atatatatta ctgaaataaa aaacacaaag aataaccaaa
    12361 ggaaattatt tgaaacgtaa aaggtatata tttttaatct gtaaaattta aaatcacctc
    12421 ctctggattg aatcaacttg ctctatttaa gacgattgtt tttacatttt caccacctgt
    12481 atcaggtggt gtttttttat tggcggcaat gcctttatct tatttttatt acgttgtaaa
    12541 tatggctatt cctaaatgaa atttgatttg ctcagtttat ttgtgatgtc aggggagtgc
    12601 ttgccatgag agtcgtgata ttat
//
LOCUS       DAJTRD010000101        12566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTRD010000101 DAJTRD010000000
VERSION     DAJTRD010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.101"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..1240)
                     /gene="uxaB"
                     /locus_tag="OO315_003272"
     CDS             complement(<1..1240)
                     /gene="uxaB"
                     /locus_tag="OO315_003272"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5892072.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQD"
     gene            complement(1467..2885)
                     /locus_tag="OO315_003273"
     CDS             complement(1467..2885)
                     /locus_tag="OO315_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5892073.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEVLKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3039..3383)
                     /locus_tag="OO315_003274"
     CDS             complement(3039..3383)
                     /locus_tag="OO315_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5892074.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3383..4309)
                     /gene="glsB"
                     /locus_tag="OO315_003275"
     CDS             complement(3383..4309)
                     /gene="glsB"
                     /locus_tag="OO315_003275"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5892075.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4373..5308)
                     /locus_tag="OO315_003276"
     CDS             complement(4373..5308)
                     /locus_tag="OO315_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5892076.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5388..6503
                     /gene="yneK"
                     /locus_tag="OO315_003277"
     CDS             5388..6503
                     /gene="yneK"
                     /locus_tag="OO315_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5892077.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6653..7843
                     /gene="ydeA"
                     /locus_tag="OO315_003278"
     CDS             6653..7843
                     /gene="ydeA"
                     /locus_tag="OO315_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5892078.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(7868..8533)
                     /gene="marC"
                     /locus_tag="OO315_003279"
     CDS             complement(7868..8533)
                     /gene="marC"
                     /locus_tag="OO315_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5892079.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            8745..9179
                     /gene="marR"
                     /locus_tag="OO315_003280"
     CDS             8745..9179
                     /gene="marR"
                     /locus_tag="OO315_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5892080.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9199..9582
                     /gene="marA"
                     /locus_tag="OO315_003281"
     CDS             9199..9582
                     /gene="marA"
                     /locus_tag="OO315_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5892081.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9614..9832
                     /gene="marB"
                     /locus_tag="OO315_003282"
     CDS             9614..9832
                     /gene="marB"
                     /locus_tag="OO315_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5892082.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(9863..10762)
                     /gene="eamA"
                     /locus_tag="OO315_003283"
     CDS             complement(9863..10762)
                     /gene="eamA"
                     /locus_tag="OO315_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5892083.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11026..12144
                     /gene="ydeE"
                     /locus_tag="OO315_003284"
     CDS             11026..12144
                     /gene="ydeE"
                     /locus_tag="OO315_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5892084.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12271..12366
                     /gene="mgtS"
                     /locus_tag="OO315_003285"
     CDS             12271..12366
                     /gene="mgtS"
                     /locus_tag="OO315_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5892085.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
BASE COUNT         3308 a         3212 c         2871 g         3175 t
ORIGIN      
        1 cgtcctgtac cggatatgtt tctccgttac gctcaccacg atagaacgca atcaatgccg
       61 ctaatgcgaa agtaaggcgc gccggaagtg tgccatttgc cttctgccct gccagcagct
      121 gcggcaggat gcgggtgcgg aacttggtca taccgttgag cgcgatagac agcagctgat
      181 gcttaatgta cgggttacgg aaacgcccgg tgactgcact ggcgaaagat tccagttcat
      241 cacgaggcaa atccagtacc ggaataattt cttcgtagat ggctttttca acgaatgcgc
      301 aaatttcagc atcgttcatc gcttcaccta cggtatccag ccctgcctgg aacgccaccg
      361 gcaccagcgc ggtgtgcgca ccgttgagga ttgccacttt gcgttcttta tacggtttaa
      421 tatcgtcaac aatcagcacg ttgagcggat atttgtccag gcgcagctca gtcgctaagg
      481 atttcggccc ctgaatcaca aacaggtaaa agtgttcagc ggtgtcgagg aaaccatcgt
      541 gataacccag ttcttcttcc agtttagcca cttcatcgcg cggataaccg gtaacgatac
      601 ggtccaccag cgtagaacag aagctgttag cctgatccag ccattgaata aatgcttctg
      661 gcagtgccca ctcttgcgca tagcgcagca ccagttcacg caacgcgtcg ccattgtagt
      721 caatcaactc acacggaatg atgatccaac ctttatccag cgcaccgttg aaatggctaa
      781 agcgttcgaa cagcagacgg gtcagttttg ccggatagct taccgctggc gcgtcatcga
      841 atttatcgcc cgcatggtag ctgatacccg cttcggtggt gttagagaaa acaaagcgca
      901 tttccgggtt gtgcgccagt ttcaggaatt catcgtattc actgtagacg ctgatttcac
      961 gattaaccga gcgaatcaga cgcgcgtcgc tgaccgcttc ccccttctca ttcaggccac
     1021 ggataatggt ggtgtacaga ccatcctgcg tgctcagtga cggcgggaat gaagtttcaa
     1081 tcggacgaac aacgaccacg ccagaattca gatcggtgtg ctcattcagg agatcgattt
     1141 gccagtcaac aaaggcgcgc aggaagttac cttcaccaaa ctgaatgata cgttctggat
     1201 actgtgcacc gggaaaatcg cgacgattta gtgttttcac aatgggttcc cttctgatta
     1261 gtcatacaac ccgtttgaat tggtgcgaca ggttagcaaa ctttaatacg ccgaacccct
     1321 gttttgatca actcctgatg attaatgagc agttttatga gaaaagtgtg gcgcggatca
     1381 tggtttaatc gaggaaatac gccttttcct ggatcataaa gtggtagaac acattgcatt
     1441 caaatcgcgc ggaatgaata aagatgtcag acaacttcct caccgtaacg catagtgctg
     1501 gtacggttgc gcccatcttt cttcgagcga tacagacacg tatcggcttc gaccatcaat
     1561 ttattaaaat catcggtcag cgttcggtac gatgcgcgac cactaccgac gccaatactt
     1621 accgtgagat aaagcgtttt ttgttgccag gtgaatggtt gcagttcaac gcctttacgg
     1681 attttttccg ccattagcag accatcgaca ggattcaccg acggcactgc aacagcaaat
     1741 tcttcgccgc ccatgcgcgc caccagcccc ttatcaccga caatcttctg aatatgccgg
     1801 gcaaacacgc ttaacacttt atcgccacat tcatgtccat agttatcgtt aatgcttttg
     1861 aaatagtcga tatcaagcag catgaccgtc agatgttgtg tctgtttcag cgatggactt
     1921 ttcagcacct cataaagacc ggaccgtgag taaacctgag tcagaaaatc aaagtcggct
     1981 cgcagcgcaa cttgcttcat tagcaaattg atcgctgcca cgctaaaaga aaccataatt
     2041 gggcatatcg ccatcgtggc aataccgaga cgtgcggaga acatttccgg aatggagaac
     2101 ggcgaaccga ccgagatatc aatcaccgaa tttgccacca gtacgatttc caccgcaccg
     2161 gtgacaaagg tcaacagaca tgttacctgc ggcgtatagc gcactgcaca ccagattaat
     2221 gcaggcagcg ggaacgccag actccccgct ccgccaatga ctaccgaggc aataaccgac
     2281 acaataaacg cgatggcggg catcatctgt tctgctttaa agcgcggcag cactccagga
     2341 atagccaacg tcagcataca aggcacgatc aacacgcccg ttgagaattg ctcactgaac
     2401 cagtccgcaa gcaaaggcca gaaatccaga ctatcaatac tgaccgaacc aatcgccccg
     2461 acaatagcgc ataataatgc ggcaatcaga cagtaattaa atagccgtaa agcacttact
     2521 ggctcatact tatttttccc cagacgctta tcccgcacga ccagtaaggc gacggtaaca
     2581 ataaacatca tattggagaa attgataacc agtgaaacca gtccccattc ggtggttatg
     2641 gcatcataaa ccagcatcgc cacataactg atcgcatagt aatgcagtcg attaagccag
     2701 acatagcggg caaacacccc cgccattacg ccattcagcg gccagaaaag agaaagttcg
     2761 tgaaccagcc gtaactctgc accaataaaa tagaacagcg tggttagcgc aaaaatagcg
     2821 atgctattac gtagcagatg accttcctgg aacaaacgga aagttgaaat ggggtgtaca
     2881 tgcattaatt atctgtccat acaattgcgg gtggcaatta tctcaacgtg ataagtaaat
     2941 tctcataaag gatagcatgt tcgctaacag cggcggcaaa taaaggtacc ctgggaatgc
     3001 tattttatcg ttttccgcaa ttctcatggc tgattcatct atacaaccag ccagtgatag
     3061 attaccgcca cgatataacg ttgttgttct tcgcttaacg aaacaccctg cggaatgtta
     3121 tgccacttag ccagacaacc gcgacagcag gtcgcggtgg cgtgctgagc gataaacacc
     3181 ggatgcccgc gcatgggggt ttgcttaccg tcattagccg gtaatgccgg agcaagacgt
     3241 ttagcgacaa aatccgccgc atgttgttcg atgactggcg cgcctttctc caggcaatac
     3301 tgacgctctt tcatgcccag acgaaagcga gagcgaaatt ttgaacggga taaacgcgcg
     3361 aagagtggat cgagagactg cattaataaa ccgaacgccc taactgtttc gtcaattgtt
     3421 caaggacggc gatacccgca agcgagttac ctgcatcatc cagttccgga ctccagactg
     3481 cgatggccat ttcatgcgga acaatcgcca caataccgcc accaacgcca gatttcgccg
     3541 gtagtcccac ccgccaggca aactcccccg cgttctggta cataccactg gtcgccatca
     3601 gcgcgttaat ttgccgcgcc tgcattggcg tcaccacagg ttcatcaata tgaatagctt
     3661 tcccctgatt agccagaaag acaaacgtcc gggccagctc tacacagctc attttcagag
     3721 cgcagtaatg aaagtagttt tgcagaacgg ttgtcacgtc atgatggaaa ttgccaaacg
     3781 acttcatcag ccaggcgata gccgcatttc gcgcggaatg ttcaaattcg gaacgcgcta
     3841 ccaccgtatc gtatgaaata tcagacacac cgcttaagcc gcgcacgact tccagcatac
     3901 gttgccgtgg tgcgcttagt cgcccttgca acatatcgca gaccaccagc gcaccggtat
     3961 taatgaacgg attacgcgga ataccctgtt ccatttccag ttgcactaaa gaattgaacg
     4021 gtgatccaga cggatctttg ccgacgcgtt gccagatttc ctcttcggag taatgacgca
     4081 tggcgacaac gagactcagc actttggaaa tagactgaat ggaaaaacgt tcttgcgcgt
     4141 ctccggcctg aaaaagctgt ccgtcaacgg tacagatagc aatccccaat cgggaaccgt
     4201 ctactgtagc cagcgccgga atataatccg cgactttacc ctgaccaatg agcggccgca
     4261 cttgccgcaa gatgttttct aaaattgcat tatccatggc gactgccact ttctactcct
     4321 ggaccgcagg tctgaaaaga cctgcgagta tatcagagct gaatatgtcg cgtcagatcc
     4381 ggtctttcca cactgtctgg atattacaga attcgtgtaa gccgaaatgg gaaagctcac
     4441 gaccaaagcc actctttttc acgccaccaa aggccactcg cgcgtcgctg gcacaataac
     4501 cattgataaa caccccaccg cattccagac gtgccgccat ctgtctggcc tgtgtttcgt
     4561 cggtggtaaa aatggtcgct gaaaggccga actcactatc attagccagt tccagcgcat
     4621 gttctgcatc tttcgcaatg gtgattgcag caacggggcc aaacatttct tcccgaaacg
     4681 cggtcatttc tggggtaaca ttcgccagaa ccgttggcgg atagtagtta cctgctccag
     4741 ccatcttttc cccgcccagt aacaaacgcg caccctgcgc cagggttttc tccacctgat
     4801 gatgcagctc atcacgtaaa tcaaaacgag ccattggtcc gagagcgttc tcttcatcac
     4861 ggggatcgcc cattttcaag gctgccgcag ctgacacaaa acgttcggta aatgccgaag
     4921 caattccctc ttcgataata aagcgttttg ccgctgcaca aacctgtccg gtattctgat
     4981 aacgtccggc taccgccgct ttaaccgcca gttccagatc ggcatcgtta agcacaataa
     5041 atggatccga accgcccagt tccagtacgc attttttcag tgccgctcca gcacgaacac
     5101 ttccggtcac cgtgacagca gcaatgcgcg agtctttaat catctgactg acaccgtcgt
     5161 tgtcggcatt cagccagcca tatacgcctt gcgggattcc cgcatcttta aacacttggg
     5221 caatgagctg agcacagccc atcacattcg gcgcatgttt aagtaagtag ccgttacctg
     5281 caagaatgat gggaacagcg ccacgcatca cctgcctttg atagttcttg cagctcaaat
     5341 gccctatata gtgatgttcc gatgacttat gactatatgg ggcaaatatg gttacgccag
     5401 taagcatctg caattacata tctcttcctg atgattttcc cgctcgtaat attgcacctc
     5461 aagtaaaaga agttttaaag gattttattg atgcactcag taccataata tgtgatgaag
     5521 agtggcgcac gtctttaaac accaactcag ccacaaaaaa gatatttaac aaccttgata
     5581 acttatcata tattcagagg acttcttttc ggggtaacga cacactatac aatgaaaagg
     5641 ttcagtttaa actcacttat cctgtcaaga atggaagaca caaagaaaat attgaatttc
     5701 aggtagtaat aaatttaagc cctatttatt tagataattt tcgccatgat ggagaaatta
     5761 atattttttg cgctcccaac ccaaagcctg tcactatggg gcgcgtattt cagaccggcg
     5821 tcgagcgtgt gctctttctg tttatgaatg attttattga acaatttcca atgatcaacc
     5881 ttggcgcccc catcaaaaga gcgcatacac cacatattga acccctgcct ccggatcacc
     5941 ataccgctgc agattactta cgccagtttg atttgcttgt cctgaatttt atctctcgcg
     6001 gtaattttgt catactcccc cgattatgga ataactctga ggttcacaga tggttcgtca
     6061 ataaagatcc taacttgatc accgccattc tcgacataac ggacagcgaa ttaaaagagg
     6121 atttattgca aagcctgatg gattcattag gttctaacaa acatgtacaa cccgaagtct
     6181 gcatctgctt cttatccctt ttagcagaac aagaatctcc tcattttcag gacttgtttt
     6241 tatttttcgc caatatgtta ttgcactatc accaatttat gaatcccaat gaaagtgatc
     6301 tgaatgacgt gttgatgcca gcatcattaa gtgatgataa aattatcaaa catatggcgc
     6361 gcaggaccct caaactgttt gtaaaaaatg aaacgccgcc aaaagttact cacgaagacc
     6421 tggtgaaaaa caggcctcgc tcccctgtca gaccacctat acccgcaacc gccaaaacgc
     6481 cagacctccc tgaacgtcat taaaccgtga tgttaccgac tctctgactc ggaacagaat
     6541 cagcgtcaga gaaacggaaa acgcgatcca gatcacaaat gcattgtatt cacatcatta
     6601 accgttttaa gatcatttca tcactttttc gcaactcacc cgataatctg ttatgacaac
     6661 aaacactgtt tcccgcaaag tggcgtggct acgggtcgtt acgctggcag tcgccgcctt
     6721 catcttcaac accaccgaat ttgtccctgt tggcctgctc tctgacattg cgcaaagttt
     6781 tcacatgcaa accgctcagg tcggcatcat gttgaccatt tacgcatggg tagtagcgct
     6841 aatgtcattg ccttttatgt taatgaccag tcaggttgaa cggcgcaaat tactgatctg
     6901 cctgtttgtg gtgtttattg ccagccacgt actgtcgttt ttatcgtgga gctttaccgt
     6961 tctggtgatc agtcgcattg gtgtggcttt tgcacatgcg attttctggt cgattacggc
     7021 gtctctggcg atccgtatgg ctccggccgg gaagcgggca caggccttga gtttaattgc
     7081 caccggtaca gcactggcga tggtcttagg tttaccgctc gggcgcattg tggggcagta
     7141 tttcggctgg cgaatgacct tcttcgcgat tggtattggg gcgcttgtta cccttttgtg
     7201 cctgattaag ttacttccct tactgcctag tgagcattcc ggttcattga aaagcctccc
     7261 gctattgttc cgccgcccgg cattgatgag catttatttg ttaactgtgg tggttgtcac
     7321 cgcccattac acggcataca gctatatcga gccttttgtg caaaacattg cgggattcag
     7381 cgccaacttt gccacggcat tactgttatt actcggtggt gcgggcatta ttggcagcgt
     7441 gattttcggt aaactgggta atcaatatgc gtctgcgttg gtaagtacgg cgattgcgct
     7501 gttgctggtg tgtctggcac tgctgctacc tgcggcgaac agtgaaatac acctcggggt
     7561 gctgagtatt ttctggggga tcgcgatgat gatcatcggg cttggtatgc aggttaaagt
     7621 gctggcgctg gcaccagatg ctaccgacgt cgcgatggcg ctattctccg gcatatttaa
     7681 tattggaatc ggggcgggtg cgttggtagg taatcaggtg agtttgcact ggtcaatgtc
     7741 gatgattggt tatgtgggcg cggtgcctgc ttttgctgcg ttaatatggt caatcattat
     7801 atttcgccgc tggccagtga cactcgaaga acagacgcaa tagttgaaag gcccattcgg
     7861 gcctttttta atggtacgtt ttaatgattt ccaggatgcc gttaataata aactgcacac
     7921 ccatacatac cagcaggaac cccatcaggc gggagatcgc ttcaatgcca cccttgccca
     7981 ccagccgcat aattgcgccg gagctgcgta ggcttcccca caaaataacc gccaccagga
     8041 aaaagatcag cggcggcgca accatcagta cccaatcagc gaaggttgaa ctctgacgca
     8101 ctgtggacgc cgagctaata atcatcgcta tggttcccgg accggcggta cttggcattg
     8161 ccagtggtac aaaggcaata ttggcactgg gttcatcttc cagctcttcc gacttgcttt
     8221 tcgcctccgg tgaatcaatc gctttctgtt gcggaaaaag catccgaaaa ccgataaacg
     8281 cgacgattaa gccgcctgca attcgcagac cgggaatcga aatgccaaat gtatccatca
     8341 ccagttgccc agcgtaatac gccaccatca tgatggcaaa tacgtacacc gaggccatca
     8401 acgactgacg attacgttcg gcactgttca tgttgcctgc caggccaaga aatagcgcga
     8461 cagttgttaa tgggttagct aacggcagca acaccaccag ccccaggcca attgctttaa
     8521 acaaatctaa cattggtggt tgttatcctg tgtatctggg ttatcagcga aaagtataag
     8581 gggtaaacaa ggataaagtg tcactcttta gctaaccttg catcgcattg accaaaactt
     8641 gaaccgattt agcaaaacgt ggcatcggtc aattcattca tttgacttat acttgcctgg
     8701 gcaatattac cccctacaac taattacttg ccaaggcaac taatgtgaaa agtaccagcg
     8761 atctgttcaa tgaaattatt ccattgggtc gcttaatcca tatggttaat cagaagaaag
     8821 atcgtctgct taacgagtat ctgtctccgc tggatattac cgcggcacag tttaaggtgc
     8881 tctgctctat ccgctgcgcg gcgtgtatta ctccggttga actgaaaaaa gtgttgtcgg
     8941 tcgacctggg agcactgacc cgtatgctgg atcgcctggt ctgtaaaggc tgggttgaaa
     9001 ggttgccgaa cccgaatgat aagcgcggcg tactggtaaa acttaccacc agcggcgcgg
     9061 caatatgcga acaatgccat caattagttg gccaggacct gcatcaagaa ttaacaaaaa
     9121 acctgacggc ggacgaagtt gcaacacttg agcatttgct taagaaagtc ctgccgtaaa
     9181 caaaaaagag gtatgacgat gtccagacgc aatactgacg ctattaccat tcatagcatt
     9241 ttggactgga tcgaggacaa cctggaatcg ccactgtcac tggagaaagt gtcagagcgt
     9301 tcgggttact ccaaatggca cctgcaacgg atgtttaaaa aagaaaccgg tcattcatta
     9361 ggccaataca tccgcagccg taagatgacg gaaatcgcgc aaaagctgaa ggaaagtaac
     9421 gagccgatac tctatctggc agaacgatat ggcttcgagt cgcaacaaac tctgacccga
     9481 accttcaaaa attactttga tgttccgccg cataaatacc ggatgaccaa tatgcagggc
     9541 gaatcgcgct ttttacatcc attaaatcat tacaacagct agttgataac gtgacaacgt
     9601 cactgaggca atcatgaaac cactttcatc cgcaatagca gctgcgctta ttctcttttc
     9661 cgcgcagagc gttgcggaac aaaccacgca gccagttgtt acttcttgtg ccaatgtcgt
     9721 ggttgttccc ccatcgcagg aacaaccacc gtttgattta aatcacatgg gtactggcag
     9781 tgataagtcg gatgcgctcg gcgtgcccta ttataatcaa cacgctatgt agtttgttct
     9841 ggccccgaca tctcggggct tattaacttc ccacctttac cgctttacgc caccgcaagc
     9901 caaatacatt gatatacagc ccggtcataa tgagcaccgc acctaaaaat tgcagacccg
     9961 ttaagcgttc atccaacaat agtgccgcac ttgccagtcc gactacgggc accagtaacg
    10021 ataacggtgc aacccgccag gtttcatagc gtcccagtaa cgtcccccag atcccataac
    10081 caacaattgt cgccacaaac gccagataca tcagcgacaa gatggtggtc atatcaatag
    10141 taaccagact gtgaatcatg gatgcggaac catcgagaat cagcgagaca acaaagaagg
    10201 gaatgattgg gattaaagcg ctccagatta ccagcgacat caccgccgga cgcgttgagt
    10261 gcgacatgat ctttttattg aagatgttgc cacacgccca actaaatgct gcagccaggg
    10321 tcaacataaa gccgagcatc gccacatgct gaccgttcag actatcttcg attaacacca
    10381 gtacgccaaa aatcgctaag gcgatccccg ccaattgttt gccatgcagt cgctccccga
    10441 aagtaaacgc gccaagcacg atagtaaaaa acgcctgtgc ctgtaacacc agcgaagcca
    10501 gtccagcagg cataccaaag ttaatggcgc agaacagaaa ggcaaactgc gcaaaactga
    10561 tggttaatcc ataccccaag agcaaattca gtggtacttt cggtcgtgca acaaaaaaga
    10621 tggccggaaa agcgaccagc ataaagcgca aaccggccag catcagcggt ggcatgttat
    10681 gaagccccac tttgatgacc acaaaattta gcccccatac gaccactacc agtagcgcca
    10741 acaccccatc ttttcgcgac attctaccgc ctctgaattt catcttttgt aagcaatcaa
    10801 cttagctgaa tttacttttc tttaacagtt gattcgttag tcgccggtta cgacggcatt
    10861 aatgcgcaaa taagtcgcta tacttcggat ttttgccatg cgtccgatgg ttaataacaa
    10921 cgacgaggca agaagggcgc tggtagagcg tcgtagggat aagttcattt gtctgtccgg
    10981 cagtgcgttt cgttatgttt tattttagag atgtaaagaa atagcatggc tacgctgcca
    11041 tttatgacca tttacttgag tcgccagtac agcctgagtg tcgatctaat cggttatgcg
    11101 atgacaattg cgctcactat tggcgtcgtt tttagcctcg gttttggtat cctggcggat
    11161 aagttcgaca agaaacgcta tatgttactg gcaattaccg ccttcgccag cggttttatt
    11221 gccattcctt tagtgaataa cgtgacgctg gttgtgctct tttttgccct cattaactgc
    11281 gcctattctg tttttgctac cgtgctgaaa gcctggtttg ccgacaatct ttcgtccacc
    11341 agcaaaacga aaatcttctc aatcaactac accatgctaa acattggctg gaccatcggt
    11401 ccgccgctcg gcacgctgtt ggtgatgcag agcatcaatc tgcccttttg gctggcagct
    11461 atctgttccg cgtttcccat gcttttcatt caaatatggg taaagcgcag cgagaaaatc
    11521 atcgccacgg aaacaggcag tgtctggtcg ccgaaagttt tattacaaga taaagcactg
    11581 ttgtggttta cctgctctgg ttttctggct tcttttgtaa gcggcgcatt tgcttcatgc
    11641 atttcacaat atgtgatggt gattgctgat ggggattttg ccgaaaaggt ggtcgcggtt
    11701 gttcttccgg tgaatgctgc catggtggtt acgttgcaat attccgtggg ccgccgactt
    11761 aacccagcta acatccgcgc gctgatgaca gcaggcaccc tctgtttcgt catcggactg
    11821 gtcggtttta ttttttccgg caacaacctg ctattgtggg gtatgtcagc tgcggtattt
    11881 actgtcggtg aaatcattta tgcgccgggc gagtatatgt tgattgacca tattgcgccg
    11941 ccaggaatga aagccagcta tttttccgcc cagtctttag gctggcttgg tgccgcgatt
    12001 aacccattag tgagtggcgt agtgctaacc agcctgccgc cttcctcgct gtttgtcatc
    12061 ttagcgttgg tgatcattgc tgcgtgggtg ctgatgttaa aagggattcg tgcaagaccg
    12121 tgggggcagc ccgcgctttg ttgatttaag tcgaacacaa taaaaattta attcagcctt
    12181 cgtttaggtt atctctgcta atatctttct cattgagatg aaaattaagg taagcgagga
    12241 aacacaccac accataaacg gaggcaaata atgctgggta atatgaatgt ttttatggcc
    12301 gtactgggaa taattttatt ttctggtttt ctggccgcgt atttcagcca caaatgggat
    12361 gactaatgaa cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg
    12421 ggcctgattt ttatgacggc gaaaaaaacc gccagtaaac cggcggtgaa tgcttgcatg
    12481 gatagatttg tgttttgctt ttacgctaac aggcattttc ctgcactgat aacggatcgt
    12541 tgacacagta gcatcagttt tctcaa
//
LOCUS       DAJTRD010000102        12561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTRD010000102 DAJTRD010000000
VERSION     DAJTRD010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.102"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(72..791)
                     /locus_tag="OO315_003286"
                     /pseudo
     CDS             complement(72..791)
                     /locus_tag="OO315_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(807..1139)
                     /gene="cusF"
                     /locus_tag="OO315_003287"
     CDS             complement(807..1139)
                     /gene="cusF"
                     /locus_tag="OO315_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5892086.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1163..2545)
                     /gene="cusC"
                     /locus_tag="OO315_003288"
     CDS             complement(1163..2545)
                     /gene="cusC"
                     /locus_tag="OO315_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5892087.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2702..3385
                     /gene="cusR"
                     /locus_tag="OO315_003289"
     CDS             2702..3385
                     /gene="cusR"
                     /locus_tag="OO315_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5892088.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3375..4817
                     /gene="cusS"
                     /locus_tag="OO315_003290"
     CDS             3375..4817
                     /gene="cusS"
                     /locus_tag="OO315_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5892089.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            4967..7204
                     /gene="nrfB"
                     /locus_tag="OO315_003291"
     CDS             4967..7204
                     /gene="nrfB"
                     /locus_tag="OO315_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5892090.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7191..10159
                     /gene="nfrA"
                     /locus_tag="OO315_003292"
                     /pseudo
     CDS             7191..10159
                     /gene="nfrA"
                     /locus_tag="OO315_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10160..11050
                     /locus_tag="OO315_003293"
     CDS             10160..11050
                     /locus_tag="OO315_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5892091.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11233..11994
                     /gene="envY"
                     /locus_tag="OO315_003294"
     CDS             11233..11994
                     /gene="envY"
                     /locus_tag="OO315_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5892092.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12040..12116)
                     /locus_tag="OO315_003295"
     tRNA            complement(12040..12116)
                     /locus_tag="OO315_003295"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12080..12082),aa:Arg,seq:tct)
     gene            12359..>12561
                     /locus_tag="OO315_003296"
     CDS             12359..>12561
                     /locus_tag="OO315_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5892093.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3057 a         3275 c         3198 g         3031 t
ORIGIN      
        1 cgagttataa cagacagtta attaactaca gtggacaatg ctagacaatg acagacacag
       61 ataagcaacc taccttcctc tttcgcgacc acgttatctt tggcgatatt cattcccgcg
      121 cgcagatcaa acgcggtgat cacgccatca atgggcgctt tgagcgtaaa gcgagtctgg
      181 attttttgcg tggcgatcag gtggcgaata tccgcctccg gcattcccgc cagtcgcagc
      241 cgctcaagaa tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcaa cagtaaatac
      301 tcactctgcg cttccaccca gtcgggaatg gtcaggtcga gaagcggtgt gcccttttgt
      361 actttatcgc ccacggtaag cggatacacc ttgtcgataa acccggcagc gcgagcctgc
      421 acaatggcat actgatactc gttgtaactg acattcgtcg ggaaactctg ggcaaaagtc
      481 agcggcccgc gcgtgacggt agccgttttc acccccagat tctgcgtctg agtcgggtca
      541 atgcgcacac cagacgcaga actctcttca tcggcatatt tcggcaccag atccatatcc
      601 ataaacggcg atttacctgg tttatcgaac cgcgtattgg gatacatcgg gtcgtaccag
      661 aataagattt tacgttctgc ggtcggcatt ttttctgcgt gcggttccgc ctggtcaaac
      721 caggtaaaac ctgccgctga aataataccg cccgcgatca tgctgccgat aataagcgcg
      781 atttttttca tctcattaaa cctgggttac tggctgactt taatatcctg taataaagaa
      841 aggttgccct gctggacaaa attaaacgcc actttgtcgc cggttttaat ttcactcatt
      901 ttcgtctgcg gggtgatggt aaagcgcatg gtcatctccg gccagttgac ggcagcaatc
      961 ggatcgtgat ggatggtgat ttttttgctt tccagatcaa caccttttac cacaccggtg
     1021 gcactaatga cctgtggttg tgcttcgctc atggtttcat gatgatgttc gttagcctgg
     1081 gcattaaagc caataacggt aaacagactg aacattgcga cttgcagtgc ttttttcatt
     1141 tattctctcc tggagttaaa aattattgct gccaaccgcc ccctaaggcg gtatacaaat
     1201 aaatttcgtt aacctgacgg gcataattca ggtcaagtaa agtttgtcgg gttgcaaata
     1261 aagaacgctc ggcatccagc acttccagat aacttaccgc gccgtgctga tataatgccc
     1321 gcgcccgttg cagggtaatt tgcagcgacg ccagataacg ctgctgggcg ctgatttgat
     1381 cgtccaggct ttgacgtaat gccagcgcat ctgccacctc tttaaaggcg ttctggattt
     1441 tctgttcata attcaccacc gactgctgct ggcgaatttc ggcgatatcc agattggcct
     1501 ggttgcgtcc ggcattaaaa atggggatct caattttagg aataaaattc cacatcccgc
     1561 tgctagcgtt aaataatgac gacagatcgc tgctggcggt tgatattccg ctggtcaggc
     1621 tgatagacgg gaaaaatgcc gcacgcgcag caccaatatt agcattagcc gccattaacg
     1681 cgtgttcagc ttccataata tccgggcgct gcaataagat ttgcgacgac aagcccgccg
     1741 gtaatttaac gctttgcagg ctgccgctgt ttactgtctg cgcttgcggt agcttgccgt
     1801 agcttcccaa taacagttgc aatgcattat tcgcctgcgc cagttccccc tgacgtttag
     1861 cgatgtcact gcgggtactt tctatcaccc cgcgagcctg ttccagcgcc agaacattgc
     1921 tgctaccggt caacagttgt ttttcgacaa acgcatatga ctgctgataa ttacgcagcg
     1981 tttcttcggc tatttgcaat tgcgcatacg ccagctgctg attgaaatag ctttgcgcga
     2041 cattagaaac cagcaaaata tgcaccgcgc gctgggcttc ctcagtggct aaataatttt
     2101 ggcgctcggc ttcgctcatg ttctttaagc gaccgaaaaa atcgagatca aagctggcgt
     2161 taaggccagt cgagaactcc cgcgtcgtgg ctgaatcgcc tttaagattg ccgctccagc
     2221 tgccgctgcc ctcgccattg agctgtgggt agcggtcggc atcggtcaga cgatattgcg
     2281 cccgtgcctc ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc accagcgcct
     2341 cgctaatcag cgtcttcacc tgattatcaa caaaaaaggt gcgccagccc acgttctggt
     2401 agttatccgc tgcgttaacc aggccgttct ggctgagtga gaactgctgc ggtacgggca
     2461 ttaccggacg ctgataatcc ggtgccagtg aacaaccagt tagcgcaagg gccacacaaa
     2521 atggcagaag tttacaagga gacataggct cataatttct ggtgatttta tgtcgccaac
     2581 tttactcgcc aggctctgat tttccggtga caggaaaatg acaaaattgt cattttgcca
     2641 ataagcgatt gccatctgat cccgctactc tagaattgcc cgggcaacat gcggaggaaa
     2701 tatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga ccaaagggtt
     2761 aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg ctgaatggct accatctggc
     2821 gatgaccggt gattatgacc tgataatcct cgatattatg ctgccagacg tgaacggctg
     2881 ggatatcgtg cgcatgttgc gctccgccaa taaagggatg ccgattctgt tgcttaccgc
     2941 gcttggcacc attgaacatc gcgtcaaggg gctggagttg ggggcagatg actacctggt
     3001 gaaaccgttc gcttttgctg aactgctggc gcgggtgcgc actctcctgc ggcgcggggc
     3061 ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg atggtcgatc tcgtcagccg
     3121 caaagtcacc cgcagcggca cgcgcatcac tctgaccagt aaagaattta ctctgctgga
     3181 gttttttctc cgccatcagg gcgaagtgct gccccgctcg cttatcgcct cgcaggtatg
     3241 ggacatgaat ttcgacagcg acactaacgc cattgatgtg gcggtgaagc tgctacgcgg
     3301 caaaatcgac aacgactttg agccgaagct gattcaaacc gtgcgcggcg tgggttacat
     3361 gcttgaggtg ccggatggtc agtaagccat ttcagcgccc gttttcgctg gcaacccgcc
     3421 tgaccttttt tatcagccag gccaccatcg cggcgttttt cgcctttgca tggatcataa
     3481 tccactcagt aaaagtgcat tttgccgagc aggacattaa tgatttaaaa gagattagcg
     3541 ccacccttga acgggtacta aatcaccctg acgaaacgca agcccgacgc ttaatgacgc
     3601 tggaagatat cgtcagtggt tattccaacg tgttgatttc cctggcagat agccacggca
     3661 aaacggtgta tcactccccc ggtgcgcctg atatccgcga gtttgcacgt gatgccatac
     3721 ccgataaaga cgcgcggggt ggcgaggtgt atctcctttc cggcccgacg attatgatgc
     3781 caggccacgg tcacgggcat atggaacaca gcaactggcg gatgattaac ttgccggttg
     3841 gcccgttggt ggacggcaaa ccgatttata cgctctacat cgcgctgtcg atcgattttc
     3901 atcttcatta cataaatgat ttgatgaata aacttattat gaccgcatcg gtaatcagca
     3961 tcctgatcgt ctttatcgtg ctgttggcgg tacataaagg tcacgcgccg atccgcagcg
     4021 tcagccgtca aatccagaat atcacctcga aagatctcga cgttcgcctt gacccgcaga
     4081 ccgtgccgat tgagctggaa cagctggtac tgtcgttcaa ccatatgatc gagcgtattg
     4141 aggatgtctt tactcgccag tccaatttct cagcggatat cgcccacgaa attcgcacac
     4201 caattactaa tctcattacg caaacggaaa tcgccctcag ccagtcgcgc agccagaagg
     4261 agctggaaga tgtgctctac tctaatctcg aagagctgac gcgaatggcg aaaatggtca
     4321 gcgatatgct gtttctcgct caggccgata acaaccagct aatccccgaa aagaaaatgc
     4381 tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt cgaggcgcta gcggaagatc
     4441 gcggcgtgga gttgcggttt gttggcgaca aacgtcaggt tgcgggcgat ccgttgatgc
     4501 tgcgtcgggc gctaagcaac ctgctttcta atgcccttcg ttatacgcca accagagaga
     4561 caattgtagt acgctgtcag acggtcgatc accaggtgca agtttccgtc gaaaaccccg
     4621 gtacgcccat tgcgcccgag cacttaccgc gattgtttga ccgtttctat cgcgttgacc
     4681 cctcccgcca gcgaaaaggt gaaggtagcg gtattgggct ggcgatagtg aaatcgattg
     4741 ttgtcgcgca taaaggcacg gttgcggtaa cgtcagatgc gcgggggaca aggtttgtta
     4801 tcacattacc cgcttaatta ttcagcaaac ggcaactttt ataaccagtg taaaaataac
     4861 gtgccgcaat agtcatacat attaatggta aaaagctgtc acaattcata aaaaacctta
     4921 atatacgcca ccctaaacat aaccagcgtt aatgtaaggt ttttgtgtgg actggcttct
     4981 tgatgttttt gctacctggc tctacggctt aaaagtaatc gcgataacgt tagcggtcat
     5041 catgttcatc agcgggctgg acgatttttt tattgatgtc gtctactggg tacgccgcat
     5101 taaacgcaag ttgagtgttt atcgccgcta cccgcgaatg agttaccgcg aactgtataa
     5161 accagatgaa aaaccgttag cgattatggt tccggcgtgg aatgaaacgg gcgtcatcgg
     5221 caatatggcc gagctggcgg cgaccacgct cgactacgaa aactatcata tctttgttgg
     5281 cacctacccc aacgaccccg atactcaacg tgatgttgac gaagtgtgcg ctcgctttcc
     5341 gaacgtgcat aaggtagtct gcgcgcgtcc tggtcccacc agcaaagccg actgcctgaa
     5401 caacgtgctg gacgccatca cccagtttga acgtagcgcc aatttcgctt ttgctggttt
     5461 tattctgcat gacgccgaag atgtgatttc accgatggaa ttgcgtctgt tcaactatct
     5521 ggtcgagcgt aaagatctga ttcagatccc agtgtatccg tttgaacgcg aatggacgca
     5581 cttcaccagc atgacttaca ttgatgagtt ttcagaactg catggcaaag atgttccggt
     5641 gcgtgaagcc ctcgccggac aagtgcccag cgcaggcgtc ggcacctgtt tcagccgccg
     5701 cgccgtgacc gcactgttag ctgacggtaa cggtattgct ttcgacgtgc agagtcttac
     5761 tgaagattac gacattggct tccgcctgaa agaaaaaggt atgacggaaa tttttgtccg
     5821 ttttccggtg gtggacgaag ccaaagaacg cgagcagcgt aaatttttac agcacgcacg
     5881 gacgtcaaac atgatctgcg tgcgcgaata tttccccgat accttttcga ctgcggttcg
     5941 acaaaaatct cgctggatca tcggcattgt tttccagggc tttaaaaccc ataaatggac
     6001 ctccagcctg acgctgaact actttctctg gcgcgaccgc aaaggggcaa tcagtaactt
     6061 tgtcagcttc ctcgcgatgc tggtgatgat ccagcttttg ctgttgctgg cgtatgaaag
     6121 tttgtggccc gatgcctggc atttcctttc tatttttagc ggcagcgcat ggttaatgac
     6181 cctgctgtgg ctaaactttg gcttgatggt taaccgcatc gtgcagcgag tgattttcgt
     6241 cactggctac tacggcctca cgcagggcct gctttccgtc ctgcgtcttt tctggggcaa
     6301 cctgattaac tttatggcta actggcgcgt gctgaaacag gttcttcagc acggcgatcc
     6361 gcgtcgcgtc gcgtgggata aaacaacgca tgacttcccc agcgtcactg gcgatacccg
     6421 ctcgttgcgc ccgttaggtc aaattctgct ggaaaatcag gtcatcactg aagaacaact
     6481 cgatacagca ctgcgtaatc gcgtcgaagg tctacgcctg ggcggttcaa tgctgatgca
     6541 ggggctgatt agcgccgagc agctggcaca ggcgctggca gagcaaaacg gcgtggcgtg
     6601 ggaatccatc gatgcctggc agatcccgtc ctcgctgatt gccgaaatgc cagcctccgt
     6661 agcgctgcat tatgcagtac tgccgctgcg tctggaaaat gatgagttaa ttgtcggcag
     6721 tgaagatggt attgacccgg tttcgctggc ggccctgacg cgtaaagtcg gacgcaaagt
     6781 gcgttacgtc attgttctgc ggggacaaat tgtcacaggg ttacgccact ggtatgcacg
     6841 ccgacgcggt cacgatccgc gggcaatgtt gtacaatgcg gttcagcatc agtggctcac
     6901 ggaacagcag accggtgaaa tctggcggca atatgtgccg catcagttcc tgttcgccga
     6961 aatactgacc acgctcggtc atattaatcg ttcagcaatt aacgtgttgt tattgcgcca
     7021 tgaacgcagt tctctgccgc tcggcaagtt tttggtcacc gaaggcgtta tcagccagga
     7081 aacgttggat cgcgtcctga caattcaacg cgaattacaa gtttcgatgc aatcactatt
     7141 actcaaagca ggtttaaaca cagaacaggt tgcgcaactg gagtccgaaa atgaaggaga
     7201 ataaccttaa tcgcgtcatc ggatggtctg gtttactgct gacgtcttta ttgagtacca
     7261 gcgcactcgc agacaatatc ggcaccagcg cagaagagct ggggctgagc gattatcgcc
     7321 attttgttat ttatccccgt ctcgataagg cgctgaaggc acagaaaaat aacgacgaag
     7381 caaccgccat ccgcgaattt gaatatatac accaacaggt gccggataat attccgctga
     7441 ctttatacct tgcggaagcc tatcgtcatt ttggtcatga tgaccgggcg cggctgttgc
     7501 ttgaggatca actgaaacgt cacccaggag atgcccgact tgagcgcagt ctggcggcta
     7561 ttccagttga agtgaaaagc gttacgactg ttgaagaact gcttgcccag caaaaagcgg
     7621 gcgacgctgc gccgaccctg cgttgtcgca gtgaagtcgg gcagaatgcc ctgcggctgg
     7681 cacaattacc tgtcgccaga gcgcaactga acgatgcgac gtttgctgca tcgccggaag
     7741 gaaaaacgct gcgaaccgat ctgctgcaac gggcaatcta cctgaaacaa tgatcccagg
     7801 cagatacgct atacaatgaa gcacgccagc agaacacatt aagcgcggca gaacgccgtc
     7861 agtggtttga cgtgcttctt gccgggcagc tggacgatcg gatcctggca ctgcaatcac
     7921 aggggatctt caccgatcct cagtcatata ttacttacgc gaccgcgctg gcttatcgtg
     7981 gcgaaaaagc acgcctccag cattatctca ttgaaaataa gccgctgttt accacgggcg
     8041 cacaagagaa aagttggctc tatctgttat ctaaatacag cgccaacccc gttcaggcat
     8101 tggcgaatta tacggtgcag tttgccgata accgccagta tgttgttggc gcgacgctac
     8161 cggtgctgtt aaaagaaggt cagtacgacg cagcgcaaaa actgctcgcc accctccccg
     8221 ccaatgaaat gcttgaggag cgttatgctg tcagcgtggc gacccgtaac aaggctgaat
     8281 ctctgcgtct ggcacgattg ctgtatcagc aagaaccgac aaatcttccc cgcctggatc
     8341 aactgacctg gcaactgatg cagaacgagc agtcacgcga agctgccgat ttattgctgc
     8401 aacgctatcc tttccagggc gatgcgcgtg tcagccagac tttaatggcg cgactggcgt
     8461 ctctgctgga aagtcatcct tacctagcaa cgccggcgaa ggtggcgatt ttatcgaaac
     8521 ccttaccgct ggcggagcaa cgtcagtggc aaagtcagtt gccgggtatt gcagataatt
     8581 gcccggcaat agttcgcttg ctgggcgata tgtcgccttc ctacgatgct gccgcttgga
     8641 accgtctggc aaagtgttat cgggacacgc tacccggtgt ggcgttgtat gcatggcttc
     8701 aggccgaaca acgacaaccg agcgcctggc aacatcgtgc ggtagcctat caggcgtatc
     8761 aggttgagga ctacgccacc gcactggcgg cctggcagaa aatcagtctt cacgacatga
     8821 gcaatgatga tctgctcgcc gccgccaaca ccgctcaggc agcgggaaat ggcgcagctc
     8881 gcgatcgctg gcttcaacag gcagaacaac gtgagctggg aaacaatgct caatactggt
     8941 ggctgcatgc gcgacgttat atcccagctc agccagaact cgcactgaac gatctcacgc
     9001 gctcaatcaa tattgcccct tctgccaact cttacgttgc gcgggcgaca atttatcgcc
     9061 aacgtcataa tgttccggcc gcggtgagtg atttgcgcgc cgcgctggaa ctggaaccaa
     9121 ataatagcaa cacccaggca gcgcttggtt acgccttgtg ggatagcggt gatatcgcac
     9181 agtcgcggga aatgctcgaa caggcgcata aagggcttcc ggacgatccg gcactgatcc
     9241 gacaactggc ctacgtgaac cagcgtctgg atgacatgcc tgcgacgcag cactacgccc
     9301 ggctcgtgat tgatgacatt gataatcagg cataacccca ctgaccccag agcaaaatca
     9361 gcaacgcttc aatttccgcc gtttgcatga ggaggtcggt cgccgctgga cgttcagttt
     9421 cgattcttcc atcggcttgc gttccggcgc aatgagtacc gctaacaata atgtcggcgg
     9481 tgcagcgcct gggaaaagct atcgtagcta cggacaactg gaagccgagt accgcatcgg
     9541 acgcaatatg ctgctggaag gcgacctgct ctcagtttat agccgcgtct ttgccgatac
     9601 cggagaaaac ggggtgatga tgccggtgaa aaatccgatg tccggcaccg gtctgcgctg
     9661 gaagccgctg cgcgatcaga tctttttcct cgccgtcgaa cagcagttgc cgctgaacgg
     9721 gcaaaatggc gcatccgata ccatgctgcg cgccagcgcc tcattcttta atggcggcaa
     9781 atacagcgac gaatggcacc cgaacggttc aggctggttt gcccaaaacc tgtacctcga
     9841 tgcggcgcaa tatatccgcc aggatattca ggcgtggacg gcagattatc gcgtcagctg
     9901 gcatcagaag gtagctaacg gacagactat tgagccttac gctcacgttc aggacaacgg
     9961 ctatcgtgat aaaggcactc agggcgcgca gcttggcggt gtcggggtcc gctggaatat
    10021 ttggaccggc gagacgcact acgacgcctg gccgcacaaa gtcagtctcg gcgtcgagta
    10081 tcaacatacc tttaaggcga ttaatcaacg taacggagag cgcaacaacg cgtttctcac
    10141 cattggagtg cactggtaaa tgcgtaagtt cattttcgta ttgctgacac tgcttttggt
    10201 cagccctttt tcctttgcga tgaaaggtat tatctggcaa ccacaaaacc gagatagtca
    10261 ggttaccgat acccagtggc aggggctgat gagtcagtta cgttttcaag gcttcgatac
    10321 cctggttttg caatggaccc gttacggcga tgcatttacc cagccagaac agcgcacgtt
    10381 attgtttaag cgtgccgcag ctgcgcaaca gactggcctg aagctgattg tcgggctgaa
    10441 cgccgatccg gaatttttta tgcaccagaa acagtcgtct gcagcgctgg aaagctatct
    10501 taatcgcctg ctggctgccg atctccagca agccagatta tggagcgccg cgcctggcat
    10561 aacgccggat ggctggtaca tcagcgcgga aattgacgac ctgaactggc gcagcgaagc
    10621 cgcccgtcaa cctttgctaa catggttaaa caacgcgcag cggctgatta gcgatgtttc
    10681 agcaaaaccg gtttatatca gtagtttttt cgccggaaac atgtcgcccg atggctatcg
    10741 ccaactgctg gaacaagtta aagcaaccgg agttaatgtc tgggtacagg atggcagcgg
    10801 cgtggataaa cttaccgctg aacagcgtga acgttatcta caggccagcg ccgattgcca
    10861 aagctccgcc ccagcaagcg gcattgttta tgaactgttt gtcgccggca aaggcaaaac
    10921 ctttacagcg aaaccgaaac cggacgcaga aattgcctcg ctgttagcga aacgttcctc
    10981 ttgcggtaaa gacatgctct atttctctct gcgctatttg cccgtcgcgc acggcattct
    11041 cgagtattaa atcccctcca ggtaagtcgg gtacgacctg gcttacctct ttcgctctcg
    11101 tcagaattcc atcaagatta attgctaaaa agcatgctaa agaaagaaga tcgttatcgg
    11161 cgaacgggcc gatcactcat gcaggaagca tagatccttt gagcggagtt tctgatatct
    11221 gaggagtgcg aaatgcaatt gagcagcagt gaaccttgcg tggtgatcct gaccgaaaaa
    11281 gaggtagagg taagcgtcaa taaccatgct acgtttaccc ttccgaaaaa ctacctggcc
    11341 gccttcgcgt gcaacaataa cgtcattgaa ctctcaacgt taaatcacgt attaatcacc
    11401 cacatcaacc gtaacatcat caacgattat ctgttgtttt taaataagaa attaacctgt
    11461 gtaaagccct ggtcgcggct agcaaccccg gttatcgctt gtcatagccg tacaccggaa
    11521 gtgttccggc tagccaccaa ccacagcaag cagcaatcca gcaaaccctg cgaagcggag
    11581 ttgacgcgcg cattactttt taccgtattg tctaactttc ttgagcaatc gcggtttatt
    11641 gccctactga tgtatatctt acgcagcagc gtccgcgaca gcgtctgccg cattattcaa
    11701 agcgatattc agcattactg gaatctgcga attgtcgcca gttcgctatg tttaagcccc
    11761 agcctgctca aaaagaaatt aaaaaacgaa aataccagct atagccagat tgtcacagag
    11821 tgtcgtatgc gttacgccgt acagatgtta ttgatggata acaaaaatat cactcaggtg
    11881 gcgcaattat gtggctatag cagcacgtcg tactttatct ctgtttttaa ggcgttttac
    11941 agcctgacac cgttgaatta tctcgccaaa cagcgacaaa aagtgatgtg gtgaagggca
    12001 aagcggaaac ggataagacg ggcataaatg aagaagaaat ggcgcgccct gcaggattcg
    12061 aacctgcggc ccacgactta gaaggtcgtt gctctatcca actgagctaa gggcgcgttg
    12121 ataccgcaat gcggtgtaat cgcgtgaatt atacggtcaa cccttgctga gtcaatggct
    12181 tttgatctgg ttgctgaaca agtgaacgac cgcgtctgat tttctgattt atttcgctat
    12241 agcggcaaac aaacgcacac cgctgcgcgt ctgaatcaag aaaacccgta ttttcatgta
    12301 tcaaagtaca atttaccgcc ctaacggaaa attgtccgct cctatgagac tggtaactat
    12361 gaaaccaacg tcggtgatca ttatggatac tcatcctatc atcagaatgt ctattgaagt
    12421 tctgttgcaa aaaaacagtg aattgcagat tgtcctgaaa acggatgatt accgcataac
    12481 catcgattat ctccgaaccc gtcctgttga tttaatcatt atggatatag acttgcccgg
    12541 aacagacggt tttaccttcc t
//
LOCUS       DAJTRD010000103        12351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTRD010000103 DAJTRD010000000
VERSION     DAJTRD010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.103"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..248)
                     /gene="yefM"
                     /locus_tag="OO315_003297"
     CDS             complement(<1..248)
                     /gene="yefM"
                     /locus_tag="OO315_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5892094.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            531..572
                     /gene="hisL"
                     /locus_tag="OO315_003298"
     CDS             531..572
                     /gene="hisL"
                     /locus_tag="OO315_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5892095.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    548..671
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            718..1617
                     /gene="hisG"
                     /locus_tag="OO315_003299"
     CDS             718..1617
                     /gene="hisG"
                     /locus_tag="OO315_003299"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5892096.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1623..2927
                     /gene="hisD"
                     /locus_tag="OO315_003300"
     CDS             1623..2927
                     /gene="hisD"
                     /locus_tag="OO315_003300"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5892097.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2924..3994
                     /gene="hisC"
                     /locus_tag="OO315_003301"
     CDS             2924..3994
                     /gene="hisC"
                     /locus_tag="OO315_003301"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5892098.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3994..5061
                     /gene="hisB"
                     /locus_tag="OO315_003302"
     CDS             3994..5061
                     /gene="hisB"
                     /locus_tag="OO315_003302"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5892099.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5061..5651
                     /gene="hisH"
                     /locus_tag="OO315_003303"
     CDS             5061..5651
                     /gene="hisH"
                     /locus_tag="OO315_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5892100.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5651..6388
                     /gene="hisA"
                     /locus_tag="OO315_003304"
     CDS             5651..6388
                     /gene="hisA"
                     /locus_tag="OO315_003304"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5892101.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6370..7146
                     /gene="hisF"
                     /locus_tag="OO315_003305"
     CDS             6370..7146
                     /gene="hisF"
                     /locus_tag="OO315_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5892102.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7140..7751
                     /locus_tag="OO315_003306"
     CDS             7140..7751
                     /locus_tag="OO315_003306"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5892103.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="OO315_003307"
     CDS             complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="OO315_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5892104.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="OO315_003308"
                     /pseudo
     CDS             complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="OO315_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="OO315_003309"
     CDS             complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="OO315_003309"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5892105.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11945..12304)
                     /locus_tag="OO315_003310"
     CDS             complement(11945..12304)
                     /locus_tag="OO315_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892106.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
BASE COUNT         3122 a         3132 c         3083 g         3014 t
ORIGIN      
        1 tcaataatgt ccttttccgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
       61 ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga attgtattct
      121 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taatgaggat cggggcatga
      181 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
      241 gtacgcatgt aaatctccta ttttgtacag ttcattgtac aatgataggt gttaattaac
      301 tatttattaa ttagtttgta gatcaagaca ttgccagcga gacgaaaaat cagactttgc
      361 tctttttggt gctgtcaagt tagaggacag tcctcttagc cccctccttt ccccgctcat
      421 tcattaaaca aatccatcgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
      481 gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagattttt atgacacgcg
      541 ttcaatttaa acaccaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
      601 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
      661 ggctttttta ttgcgcggtt gttaacggtt cagacaggtt taaagaggaa taagaaaatg
      721 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
      781 cgcgaattac tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg
      841 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgatgacat tccctgtctg
      901 gtaatggacg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
      961 cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccctgcgtcg tctggatttc
     1021 ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc
     1081 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
     1141 aaaggtatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc gccgcgtgcc
     1201 gggctggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc
     1261 ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa
     1321 atggaagaat ctaaacagca actgatcgac aagctactga cccgtattca gggtgtgatc
     1381 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc
     1441 atcgccctgc tgccaggtgc cgaacgccca accattctgc cgctggcggg tgatcaacag
     1501 cgcgtagcga tgcacatggt gagtagcgaa accctgttct gggaaacgat ggaaaaactg
     1561 aaagcactgg gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg
     1621 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
     1681 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt aacgatattc
     1741 tcgataacgt gaaaacgcgt ggcgatgagg ccctgcgtga atacagcgcg aagtttgata
     1801 aaaccacggt taccgcgctg aaggtgtctg ctgatgagat cgccgccgcc agcgaacgcc
     1861 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gagtattgaa accttccaca
     1921 ctgcgcaaaa actgccgccg gtagatgtag aaacgcagct aggcgtgcgt tgccagcagg
     1981 tcacgcgccc ggtggcttca gtgggtttgt atattccggg cggctccgcc ccgctcttct
     2041 caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
     2101 gctcaccgcc gccgattgcc gatgaaattc tttatgcggc gcagctgtgc gatgtgcagg
     2161 acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg
     2221 tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc
     2281 aggtaagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc
     2341 tggtgattgc tgacagcggc gctacgccgg attttgtggc ttctgatttg ctttctcagg
     2401 ctgaacacgg ccctgactca caggtaattt tactgacccc cgatgctgat atggcgcgtc
     2461 gcgttgccga ggccgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accacccgcc
     2521 aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtggagatct
     2581 ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgc gaactggtcg
     2641 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg
     2701 actacgcctc cggcaccaac cacgtgctgc cgacttacgg ttacaccgcc acctgttcca
     2761 gcctcgggct ggcagatttc cagaaacgca tgaccgtaca ggaactgtcg aaagaaggtt
     2821 tctccgcgct ggcttcgacc attgaaacac tggccgccgc cgagcgcctg accgcccaca
     2881 aaaatgccgt tactttgcgt gttaacgtcc ttaaggagca agcatgagca ccgtgactat
     2941 tactgattta gcgcgtgaaa acgtccgcaa cctgacaccg tatcagtcgg cgcgtcgtct
     3001 gggcggtaac ggcgacgtct ggctgaacgc caacgaatat cccacagccg tggagtttaa
     3061 gcttactcag caaacgctca accgctaccc ggaatgccag ccaaaagcgg tgattgaaaa
     3121 ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg gcgcggacga
     3181 aggtattgaa ctgctgatcc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
     3241 cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggtgtcg aatgccgcac
     3301 agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca agctggacgg
     3361 cgtaaaagtg gtctatgttt gcagccccaa caaccccacc gggcaactga tcaatccgca
     3421 ggattttcgc accctgctgg agctaacccg cggtaaggcg attgtggttg ccgatgaagc
     3481 ttatatcgag ttttgcccgc aggcatcgtt agctggctgg ctggcggaat atccgcacct
     3541 ggcgattttg cgtacccttt cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac
     3601 gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atccccccct acccgctctc
     3661 gacgccggtt gccgacattg ccgcacaggc gttaagcccg cagggaatcg tcgccatgag
     3721 cgaacgggtg gcgcaaatta ttacagaacg cgaatacctg attgccgcac tgaaagagat
     3781 cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc gctttaaagc
     3841 ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
     3901 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
     3961 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
     4021 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
     4081 ctcgcctttg aaccgggcgt gatcccggaa ttgctgaagc tgcaaaaagc gggctacaag
     4141 ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca agcggatttt
     4201 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa
     4261 gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa
     4321 ctggtggagg gttatctggc tgagcaagcg atggatcgtg ccaacagtta tgtaattggc
     4381 gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac
     4441 cgcgaaaccc tgaactggcc aatgattggc gagcaactca ctaaacgaga ccgttacgcc
     4501 catgtagtgc gcaacaccaa agagacgcag attgacgtcc aggtgtggct ggatcgcgaa
     4561 ggtggcagca agattaacac cggcgttggc ttctttgatc acatgctgga tcagatcgcc
     4621 acccacggcg gtttccgcat ggaaatcaat gtcaaaggcg acctctatat cgacgatcac
     4681 cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac
     4741 aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc
     4801 gcactggata tctctggtcg cccgcacctg gaatataaag ccgagtttac ctaccagcgt
     4861 gtgggcgatc tcagcactga aatgatcgaa cacttcttcc gttcgctctc ttacaccatg
     4921 ggcgtgacac tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agaaagcctg
     4981 ttcaaagcct ttggtcgtac cctgcgccag gccatccgcg tcgaaggcga caccctgccc
     5041 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga tactggctgc gccaacctga
     5101 actctgtgaa gtctgccatt gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg
     5161 acattgtgtt gctggccgat aaactgtttt tacccggcgt cggcaccgca caagcagcga
     5221 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
     5281 tgggcatctg cttagggatg caactcctgg gacgacgcag cgaagagagc aatggcgtcg
     5341 acttgctggg catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctgccactgc
     5401 cacatatggg ctggaaccgc gtttatccgc aggcaggcaa ccgcctgttt caggggattg
     5461 aagacggcgc gtacttttac tttgttcaca gctacgcaat gccagtcaat ccgtggacca
     5521 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
     5581 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc
     5641 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
     5701 tcagggcgat tacggcaaac aacgcgatta cggtaacgac ccgctgccgc gcttacagga
     5761 ttacgctgcg caaggtgccg aagtgttgca cctggtggat ctgaccgggg caaaagatcc
     5821 ggctaagcgt caaatcccgc tgattaaaac cctggtcgcg ggcgttaacg tcccggtgca
     5881 ggttggcggc ggtgttcgta gtgaagaaga cgtggcggcg ttactggaag ctggcgttgc
     5941 gcgcgtggtg gtcggttcca ccgcagtgaa atcaccggac gtagtgaaag gctggtttga
     6001 acgtttcggt gccgatgcgc tggtgctggc actggatgtc cgtattgacg agcaaggcaa
     6061 caagcaagtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt
     6121 ggaaacctat ctgcccgtcg gcctgaaaca tgtgttgtgt accgatatct cgcgcgacgg
     6181 cacgctggcg ggatctaacg tctctttata tgaagaagtg tgtgccagat atccgcaagt
     6241 ggcgtttcag tcctccggcg gcattggcga cattaacgat gttgctgccc tacgtggcac
     6301 tggcgtgcgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca ccgtgaagga
     6361 ggccatcgca tgctggcaaa acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg
     6421 gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc actggcaaaa
     6481 cgctacgccg aagaaggtgc agatgaactg gtgttctacg atatcaccgc ttccagcgat
     6541 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt
     6601 tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
     6661 gataaaattt ccatcaactc ccctgcgctg gcagacccaa cattaattac tcgcctggcc
     6721 gatcgctttg gcgtgcagtg tattgtggtt ggtattgata cctggtacga cgccgaaacc
     6781 ggtaaatatc atgtaaatca atataccggc gatgaaagcc gcacccgcgt cactcaatgg
     6841 gaaacgctcg actgggtaga ggaggtgcaa aaacgcggcg ccggagaaat cgtcctcaac
     6901 atgatgaatc aggacggcgt gtgtaacggt tacgacctca agcagctgaa aaaagtgcgt
     6961 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctg
     7021 gaagccttcc gcgatgccga cgttgacggt gcgctggcag cttccgtatt ccacaaacaa
     7081 ataatcaata ttggcgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
     7141 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacggt ctgatgccgg
     7201 tgattgtgca acacgcggta tctggcgaag tgttaatgct gggctacatg aacccggaag
     7261 ccttagacaa aaccatcgaa agcggtaaag tcaccttctt ctcgcgcact aaacagcgac
     7321 tgtggatcaa aggcgaaacc tcgggcaatt ttctcaatgt ggtaagtatt gccccggact
     7381 gcgacaacga cacattactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca
     7441 ccagtagctg cttcggcaac accgctcacc agtggctgtt cctgtatcaa ctggaacaac
     7501 tgctcgccga acgtaaatat gccgacccgg aaacctccta caccgccaaa ctgtatgcca
     7561 gcggcactaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag
     7621 caacggtaca tgatcgcttt gagctgacca acgaggcatc tgacttgatg tatcacctgc
     7681 tggtgctgtt gcaggatcag gatctggatt taacgacagt aattgagaac ctgcataaac
     7741 ggcatcagtg agttacgggc tgagcaggtg tgatgttgtt gtcacacccg gagccattgc
     7801 ccgatgcgca aacttatcgt gcctacacat tatctcaaca cacgtaggcc ggataagatg
     7861 cgccagcatc gcatccggca aaaaaaacgg gcatggtgtc accaccctgc ccgtttctct
     7921 taaatgcaca ataatattac ttcgcgttgt aattacgcag agcgttacgc cccaacacaa
     7981 tccccgcgcc aaccatgccg cccagaagca ctgccagaat caaggtgatt gcctttttcg
     8041 gactatcgcg acgaataggt aacgtcggtt tcatcacata gcggtaagca tgaatatcaa
     8101 gatcatcaaa ctttaatttt tcaatatcca acaggttttg acgtgtctga tagtagtttg
     8161 gtgagaacac caacggacga gtcgcttcat gcttaatcat cgactccagc gcttcgctcc
     8221 ctagaaggaa caacgtatct tgcgtcacat cttcagtctg ctgaacctgt ggctttgtca
     8281 cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc agatctttct
     8341 gctcctgcgc gaccacttcc tgggttctta aagagtcctg caagtttttc cgtcccagag
     8401 caatgttgtc cttgagatcc ttttctagct cttgattcac tttatcatca acttgctgaa
     8461 tgtattgggc caacttcatt tgtgcgccct ctgcagtttg cccaacataa gaaacagtca
     8521 atggtaattg ctggttctta acagaaggtt cgatggtaag tttttctggc tcttcctgat
     8581 tatccagcgt ttctgctaat gcagagaagg cagaactgaa gcgaccaatt aacgtctcct
     8641 gcaaatccga tactttcggt gcagcctgac catagataac attcatggca ttgttatagc
     8701 cagcaatttg ccccacgtcg ggctgagtga taattgctgt tgacgtccat ttctccttcg
     8761 ctactgccaa ataaccaata gccagggcaa tagccacaat gacggaaatg ataattgtca
     8821 tcttgccacg ccacaactgc actagtaaat caatcaaatc aatctgttcc gggtcatggt
     8881 tttgcccaga aacattatta ttttctactc tcatcattac cctaactgac ggaaatttcc
     8941 aagtccgcat agtttagcga taatccccgt gaatgctata ggataagtga taaaaataat
     9001 gaattattgc ggaggtaaca aaagacaatc agggcgtaaa tagccctgat aacaagatgt
     9061 tagtcgctgc caaagagatc gcgggtgtag accttatctg ccacatcctt aagctcttct
     9121 gccatacggt tggagataat gacgtcggct tgttgtttga aggtggcgag atcacgttcc
     9181 aggcgagagt tgaagaatga gtcttctttc atcaccggct cgtagatgat cacttcaacg
     9241 cctttcgcct tgatacgctt cataatcccc tgaatagaag acgcacggaa gttatctgac
     9301 ccgctcttca taatcagacg ataaataccc accacttgcg gtttgcatcg acaattgccg
     9361 agatcaggtt attcggcaca gactggtagt tcgccagtaa ctgcttggta tctttcggca
     9421 gacaataacc accataacca aacgacggat tgttgtaatg gttgccaata cgcggatcga
     9481 gacaaacgcc ttcgataatc tggcgagtat tcagacctaa actttctgca taactatcca
     9541 gttcattaaa gtatgctacg cgcatcgcca gataggtatt agcgaaaagt ttaatcgctt
     9601 ctgcttcagt ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta
     9661 acaacgcagc aaaacgttct gcgcgttctg aacgctcacc gatgacaata cgtgacggat
     9721 ggagattatc gtaaagggct ttaccttcgc ggaggaattc cggagagaaa ataatatttt
     9781 cagtacgata tttcttatgc atcgctgcgg taaaaccaac gggaaccgtt gatttgatga
     9841 ccataaccgc ataaggattt atctcaacta cgtctttaat tactgattct acactggatg
     9901 tattgaaata attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag
     9961 catcccggta ggcttcattt ttatctaatg tggcattaaa gtgtatttta tctgattgca
    10021 aaaactgctg aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc
    10081 gtgacggtaa aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg
    10141 acaagcctac atagccagta ccggaaatgg tgattttcat ttcatgctct cagaattaac
    10201 ttaactgtga atcatgatgt ttttagcatc gtgataagat gctaaaagtt ttaacgctac
    10261 gttgtcagat ggtcaacgca acgaaaaaca attcagattt tctttataag aatagctgat
    10321 gtttattaag ttaatattaa gcagtgaaat ttagtctata agatatttgg caaaaaaaag
    10381 cccggtgcaa tatacgccgg gcctcaattt tattgttggt taaatcagat taatccagcc
    10441 attcagtatg gaacacacct tctttatcaa tgcgcttata agtatgcgcc ccgaaatagt
    10501 cacgctgtgc ctggatcagg ttcgcaggca gaaccgcggc acggtagcta tcgtaatagg
    10561 caaccgcagc ggcgaaggtc ggaaccggga taccgttctg tactgcataa gcgacgacat
    10621 cgcgcagcgc ctgctggtag tcatcggcaa tttgcttgaa gtacggggcc agcagcaggt
    10681 tagcgatctg cggattttcg gcataagcat cggtgatttt ctgcaggaac tgcgcacgga
    10741 tgatgcagcc agcacggaaa atcttcgcga tttcgccgta gttcagatcc caattgtatt
    10801 cttcagacgc agcacgcagc tgagagaagc cctgagcgta agaaacgatt ttgcccagat
    10861 acagcgcact gcgaactttt tcgatgaact cagccttgtc gcctgctgac tgagcttgcg
    10921 gaccagagag aactttagat gcggcaacgc gctgatcttt cagagaagag atataacgtg
    10981 caaacacaga ctcggtaatc agcgacagcg gttcgccgag atccagcgcg ctctggctgg
    11041 tccatttacc ggtgcctttg tttgctgctt catccagaat cacatcaacc aggtagttac
    11101 cgtcttcatc ttttttggtg aagatatctt tagtgatgtc gatcaggtag ctgctaagtt
    11161 caccgttatt ccactcggtg aaagtctggg ccagttcttc attagataga ttcaggccac
    11221 ctttgagcag agaatacgct tcagcaatga gctgcatatc tccgtattca ataccattgt
    11281 gaaccatctt cacataatgg cctgcgccgt cggcaccaat ataagtaacg catggctcgc
    11341 cgtcttctgc cacggcagcg attttagtca ggataggcgc aaccagctca taagcctctt
    11401 tctgcccgcc aggcataata gaaggacctt tcagcgcgcc ctcttcaccg ccggaaacac
    11461 cggtaccgat gaagttaaag ccctctgctg aaagctcacg attacgacga atggtgtcct
    11521 ggaagaaggt attaccacca tcaatgatga tgtcaccttt atcgagatat ggtttcaggg
    11581 aatcaatcgc agcatccgtg cctgcacctg ctttcaccat taacaggatg cgacgaggcg
    11641 tttccagaga ttcaacaaac tctttcaccg tatagtaagg agccagtttc ttgcctggat
    11701 tttcagcaat cacttcttcc gtcttttcgc gggagcggtt gaaaatagag acggtataac
    11761 cacgactttc gatgttgagc gcaaggttgc gccccatcac tgccataccg actacgccga
    11821 tctgttgctt tgacattgtt tactcctgtc ttctatatta ttaaccgtct attaaccgtt
    11881 atattattaa ccgtcgtgat tttcatcttc taatcaagga atacatatgg ttacagcatg
    11941 cttcttactt tatagcgata agtatgccat tgggtgtatc cataagaact ttgaattttg
    12001 gatacttttc tggattaaaa ttagcatagc ttcgtttaga tataacaata aacgaactat
    12061 taaaagattt catcttctct attaaatcat cctgtgggat tacctcacac tcgggattac
    12121 taatagtcca atcactatat cttgctaatc caaagacgtc accaattgcc atccctttat
    12181 agaagtaaat accatcctcc aagcctaatt ggataagatt atctccatat aactcactat
    12241 attcacttgc tttcttcatc agttcatagc tatagcgact ttctaagcgt gaattaaaat
    12301 tcaagtattc agaataaaaa tatttgaatt caaaatacaa cgatcgcccc g
//
LOCUS       DAJTRD010000104        12220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTRD010000104 DAJTRD010000000
VERSION     DAJTRD010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.104"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..493)
                     /locus_tag="OO315_003311"
     CDS             complement(<1..493)
                     /locus_tag="OO315_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5892107.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OO315_003312"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OO315_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5892108.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="OO315_003313"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="OO315_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5892109.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="OO315_003314"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="OO315_003314"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5892110.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="OO315_003315"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="OO315_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5892111.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="OO315_003316"
     CDS             3647..4156
                     /locus_tag="OO315_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5892112.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="OO315_003317"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="OO315_003317"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5892113.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="OO315_003318"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="OO315_003318"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5892114.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="OO315_003319"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="OO315_003319"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5892115.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="OO315_003320"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="OO315_003320"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5892116.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="OO315_003321"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="OO315_003321"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5892117.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="OO315_003322"
     CDS             complement(8798..9691)
                     /locus_tag="OO315_003322"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5892118.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="OO315_003323"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="OO315_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="OO315_003324"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="OO315_003324"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5892119.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11581..>12220
                     /gene="allE"
                     /locus_tag="OO315_003325"
     CDS             11581..>12220
                     /gene="allE"
                     /locus_tag="OO315_003325"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5892120.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3158 c         3118 g         2978 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatacaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 ccacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catccggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgct
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgttg attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgccagcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggcgc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcaggaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12061 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12121 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12181 gaacatggtg cctatattct ttccggtcag ggggtttata
//
LOCUS       DAJTRD010000105        12112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTRD010000105 DAJTRD010000000
VERSION     DAJTRD010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.105"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..369
                     /locus_tag="OO315_003326"
     CDS             <1..369
                     /locus_tag="OO315_003326"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892121.1"
                     /translation="TTLMSWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPS
                     GSVTKILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNA
                     LINLLAQQSRFADLKLSNYK"
     gene            complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="OO315_003327"
     CDS             complement(457..1101)
                     /gene="cheZ"
                     /locus_tag="OO315_003327"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5892122.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="OO315_003328"
     CDS             complement(1112..1501)
                     /gene="cheY"
                     /locus_tag="OO315_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5892123.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1516..2565)
                     /gene="cheB"
                     /locus_tag="OO315_003329"
     CDS             complement(1516..2565)
                     /gene="cheB"
                     /locus_tag="OO315_003329"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5892124.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="OO315_003330"
     CDS             complement(2568..3428)
                     /gene="cheR"
                     /locus_tag="OO315_003330"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5892125.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3447..5048)
                     /gene="tap"
                     /locus_tag="OO315_003331"
     CDS             complement(3447..5048)
                     /gene="tap"
                     /locus_tag="OO315_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5892126.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5094..6755)
                     /gene="tar"
                     /locus_tag="OO315_003332"
     CDS             complement(5094..6755)
                     /gene="tar"
                     /locus_tag="OO315_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR5892127.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6900..7403)
                     /gene="cheW"
                     /locus_tag="OO315_003333"
     CDS             complement(6900..7403)
                     /gene="cheW"
                     /locus_tag="OO315_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5892128.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7424..9388)
                     /gene="cheA"
                     /locus_tag="OO315_003334"
     CDS             complement(7424..9388)
                     /gene="cheA"
                     /locus_tag="OO315_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5892129.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9393..10319)
                     /gene="motB"
                     /locus_tag="OO315_003335"
     CDS             complement(9393..10319)
                     /gene="motB"
                     /locus_tag="OO315_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5892130.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10316..11203)
                     /gene="motA"
                     /locus_tag="OO315_003336"
     CDS             complement(10316..11203)
                     /gene="motA"
                     /locus_tag="OO315_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5892131.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11330..11908)
                     /gene="flhC"
                     /locus_tag="OO315_003337"
     CDS             complement(11330..11908)
                     /gene="flhC"
                     /locus_tag="OO315_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5892132.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11911..>12112)
                     /locus_tag="OO315_003338"
     CDS             complement(11911..>12112)
                     /locus_tag="OO315_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5892133.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2783 a         3387 c         2934 g         3008 t
ORIGIN      
        1 actacgctaa tgagttggga tgccgtgctc tatgatctac gtagtgaaaa aattatcagt
       61 aaagataatt acttgaccga cttaaaccgc agacatcttg aacttgttct tcatactaac
      121 cccaatccaa gcggttcggt aacgaagatt ttacgcacta tttttactaa gcaggtaaag
      181 agtttagggc cggaactctg tcatttcttg caccatgaac tgacgaaaaa acgttatgaa
      241 gtatggcatc actacgatgt actgcactac cagtcatcgt cattttctcg ttcacaattt
      301 aatgcattga taaatttact ggcgcaacaa tcacgcttcg ctgatctaaa attatcaaac
      361 tacaaataac catcctccac gtactccctg aacgataccc gccaccctgc tatcggcttg
      421 gcatcatcgc gcaggccgaa gttcaggcaa tacaaatcaa aatccaagac tatccaacaa
      481 atcgtccacc tgatcctgac tggctaccac gccggctttg ctggtatcga cctgaggtcc
      541 attaagcaaa ctctggtttt cacgttttgg acgcgactcc tgttccggga tgttttccaa
      601 cagcaccatc agcaactggc gttcgatctc ctgaatgaca tccatcatcc gcttaataac
      661 ctgcccggtg agatcctgaa aatcctgcgc catcatgatt tccagcagtt gcgcgttagt
      721 aaagctggta tgcgcgggta catctgccag aaattgtcgt gtatctgtta ccagttcacg
      781 ggcgtcggca aggtcaatcg gatcggcaaa ccagtcatcc caacgttggg ttaacgcttt
      841 tgctgatttc tccatttgat cctgatgcgg ttgtgacgcc tcaacactgt tcagcgcccg
      901 ctccgcagcc tgggcggtca tctgcacaac atagtacaaa cgatcgcgcg catcggggat
      961 ggcttccgcc gcttcggcaa tggcctgaac cagccccagt tcccgcaaac tgtcgcgcag
     1021 catatgcgtc aggctgccga tgcgcgcaat gatatcgcca gctgaatgct cgtcagcagg
     1081 tttgattgat ggttgcatca tagtcgcatc ctcacatgcc cagtttctca aagattttgt
     1141 tgagtttttc ctccagcgtc gcggcggtaa atggcttcac cacatagcca ctggcccccg
     1201 cttgcgccgc agcaatgatg ttctctttct tcgcttctgc agtcaccatt aacactggca
     1261 atgccgacat cgcgccatcc gcacgaattg ttttcagcaa ttccaggcca tccatattgg
     1321 gcatgttcca gtcggagata acaaatccat aaccgcctgc ctgcaactta ttgagagcgt
     1381 cgacaccatc ttccgcttcc tcaacattat tgaatcccag ctctttcagc aggttacgca
     1441 ctatgcgtcg catggtggaa aagtcatcca caaccaaaaa tttaagttct ttatccgcca
     1501 tttcacactc ctgatttaaa tacgtatcgc ctgtccggca ctaattgttg ccaacatttg
     1561 ctggcttacc tggctaagat cgaccacttc gcagacacca cccatattga tggcctcgcg
     1621 cggcatgccg aacaccacgc aacttgcttc gttttgcgca agggtccatg cccccgcctg
     1681 acgcatcgcc aacattcccg ccgcgccgtc gttgcccata ccggtcagga tcaccccaac
     1741 cgcattacgc cccgcctgtt tggcgacaga atggaacaac acatctaccg aaggccgatg
     1801 acggttaacc gccgggccgt cgtgaatttt gatttgataa tttgcgccac tacgcgccag
     1861 ttccatatgc cgatcgcccg gcgcaatata ggcatgcccc ggcaagacac gttctccgtc
     1921 ttcggcttct ttaaccccga tctggcaaag cttattaagt ctgtcggcaa aagagcgggt
     1981 gaaaccgggc ggcatatgtt gggtaattaa caatgccggg ctggaaagcg gcaacggttg
     2041 cagtacgtga cgaattgcct cagttccacc cgttgaagca ccaatcgcaa tcagtttttc
     2101 agaactcaac aacggccctg ccttcagcgt tgtcggtgct gacaatggct tatgtgctgc
     2161 aaggctcgcc tttgctgccg tacgcacctt ttcagcaatc atttcgctat acgccagcat
     2221 tccttcgcga atacccagtt gcggtttggt gacaaaatct attgccccca gctccagcgc
     2281 gcgcagcgtg acttctgatc ctttgccggt caaggaagaa accatcacaa cgggcattgg
     2341 acgcaaacgc attaattttt cgaggaaatc cagtccgtcc atccgcggca tttcaacatc
     2401 cagcgtcagc acatcgggat tgaatttctt aatcaagtca cgcgcaacca gcggatcagg
     2461 cgcggtcgcc accatttcca tgtcgctatg gctgttgatg atttctgtca tgatctggcg
     2521 catcagtgcc gaatcatcga cagataacac cctgattttg ctcatcgtta atccttactt
     2581 agcgcataca ccgtctgacc acgcagcgtg aagcggcgct cgaggtggct aaagttttca
     2641 gagtgacccg caaacagcaa tccgtcgggt ttaaggagcg gaacaaagcg gcgcaaaatc
     2701 tcctgctggg tagtttgatc gaagtagatc atgacgttac gacagaagat cgcatcaaac
     2761 ggccccggca cggtgtactg tttcgccagt aaattcagcg gggcaaaatc aacatagttc
     2821 gccagctcct gacgcacgcg taccagccct tcatgcggtc ccgtccctcg catgaaatac
     2881 cgttgcagtt gctgcggcgt caggtttttc aactcttcat ggcgatagat accgcttctg
     2941 gctttttcca gcacttcggt gtcgatatca ctggcaaaca ctttccagcg tccgggcacg
     3001 gtgcccaatg tgtcagccag agtcatcgca atgctgtacg gctcttcgcc ggtcgaagcc
     3061 gccgcgctcc atacgcgatc ctcgccagaa cggcgacgtg cgtgatccgc gagcagaggg
     3121 aaatgatgag cctcacggaa aaatgccgtc agattcgtgg tcagcgaatt gataaacgcc
     3181 tgccactcac cgctgtgctg attagattcc agcaagttca gataatgacc gaaatccgtc
     3241 agtcccagag aacgcaaacg acgaaccagt cggttgtaaa ccatgtcgcg tttatggtca
     3301 gccagaacga tcccggctcg ttgatagatc aattgactta tccgccgaaa atgcgcgtcg
     3361 gaaagcgcca ggcgctcggt catctgtaac aataaagacg tttgcccaca gggcagagat
     3421 gaagtcatag cgccttctca atcacttcag gataccactg gcgcaatttg taactgcgcc
     3481 gactcatgtc gtgctacttc atgttcttca agggtaaata ccgccacacg cgacgaaaga
     3541 tggtcggcct gattcgccag ttgttccgtt gccaccgccg cttcttctac cagcgaggag
     3601 ttctgctgag tcacctgatc catctggctg acagcctgtg caacctgctc aatcccccgc
     3661 cgttgttctt ccgacgccga agcaatttct cccataatgt cgttcacgcg ggtcaccgaa
     3721 ctgacaatat cggtcatggt cgcggcggcg ttattcacca gtttcgaacc ctgctggaca
     3781 cgattgactg actcttcgat cagccctttg atctcttttg ctgcctgcgc gctacggctg
     3841 gcaagattgc gcacttcacc tgccactacc gcaaaaccac gcccctgctc tccggcgcga
     3901 gccgcttcca ctgccgcatt cagggccaga atattggtct ggaaagcaat tccgtcgata
     3961 acgctgataa tgtcgccaat tttttgcgag ctggtggcga tctcctgcat ggtgtgagtc
     4021 atggtactga cctgaacacc gcccgcctgc gccgttgtcg cggcattttt tgccagttcc
     4081 gacgcctgtc gtgcgttatc ggcgttttgc cctaccgtgg cggttaattg ctccatactg
     4141 gcggccgttt gtgccagcga tgccgcctgc tgttcggtac gacttgagag atcgttattg
     4201 cctgcgacaa tctccgcgat cccaatgtgc atctcatggc ttcccttacg cacatcactc
     4261 accgtcccac gcaaagcctg ctgcatggtc ttcaggctgg caaaaatggc ggtgatctca
     4321 ttacgaccat ataccgcaat cggacgcgcc agattacccg cagcaatgct gtcaaaatgg
     4381 ctaccgataa tggccagtgg ttgaacaatc atcttgcgcg tccaccacag cgcactgctg
     4441 atgtagatcg ccgcaacaat aatcatgctg ataaacacca gcgccgaaat ctgatagtta
     4501 cgctggcttt gcgcactggc ggcttccagc acatggttga tctccagctg ccaggcctca
     4561 aagttaacgt caaacgcatt ctgtgatccc tgcaccggcg cagtgaggaa atccgaaagt
     4621 tgattacttt caagccaggt cgcctgatgt tcgagatcgt tgtgccagcg ggcaaaactt
     4681 ttctccgtct ctttttgcaa tgccctgacg tgctcgttgc ccgcagtcat cgccataaaa
     4741 cttttaaaca gcgtggtgga ttgcgtcaga ctggcgcgcg ccgtcgtcat caacgtttta
     4801 atgtcatccg ccggatagct aagcgctgtc agagtgcctg ctttgttcag cgcggtactg
     4861 gcctgtaaca ttaccgcccg agtttgcgct aatgccgcac gttgctgatt gctctgctcg
     4921 ccgtgattca gtcgttgcaa atcgtcgcga aatgcccaaa aagacatgcc gttactgcca
     4981 atctgcaaga tcccgcagag aatcaaaatt aaaaacagcg tggtcgaaat tcgaatacga
     5041 ttaaacatca acgctcccca tcaggcggca atgaccgcgt tagtaaaaac tcgtcaaaat
     5101 gtttcccagt ttggatcttg ttcagtaatt cgcagtcgtg gctgtgccgg tggttgctca
     5161 ctggcaggac gggatggtgt ttgcggttta ttggtgagtg ggctggctgc cagacggaac
     5221 gcggaaaccg cttgcgttaa acgactcgcc tgttcttcca gcgcagctgc ggcggcagct
     5281 gattcctgca ccagcgatgc gttctgttgc gtgacgcgat ccatttccga aaccgccaat
     5341 gcgacttgat cgatgccacg gctctgttca tccgatgccg atgcaatctc gcccataatg
     5401 tcagtcacgc gagtgacagc attgacgata ttgttcattg tttccccggc gctttcgacc
     5461 agcaccgaac cggtatcaat gcgcgagacg gagtcttcaa tgagggcttt gatctctttt
     5521 gccgccaggg cgctgcgact ggcaagatta cgcacttcac ccgccaccac ggcaaaacca
     5581 cggccctgtt cacccgcacg cgcggcttca accgcggcat tcagcgcgag gatattagtc
     5641 tggaaggcaa taccgtcgat aacgctgata atgtcggcaa ttttcttcga actatcggcg
     5701 atctcatgca tcgttttcac tacgccatcc accactttgc cgccgtgctg ggcggtgtcg
     5761 gaggcacttt gcgccagttg cgaggcctgg cgggcgttat cggcgttttg cttcactgtc
     5821 gcggtgagct gctccatgct ggcggcagtt tcttccagcg cggatgcctg ctgttcagta
     5881 cgggaggaaa gatcggtgtt gcccgccgca atttcacggg taccggcata gatggcatct
     5941 gaaccttcgc ggacatgagt gacggtgtca gtcaaagagc gttgcatatg tgaaacgctc
     6001 tgcgccaggt cacccatttc actgcgcccg tcaatggtca gggtattcgc caggttacca
     6061 ccggcgattt cgcaaatgtg agcaataatt tttgccagcg gagtaagcaa catacggcga
     6121 atgccgtacc acgccaccag cagaatcaat accaccacca gcgcgataac cgccagttgc
     6181 cactgggcaa atcggtaatc atctgcgttg tcagtgacga tatcgcgata cagtttttca
     6241 ctgctgaggg cgtactgagc aaacgcttcg cccattgcat tttgcattcc ctgggttggc
     6301 tgagcgaaat aagctccagt attgccataa tcaagataat caatcagttc agttaacgct
     6361 gtgtaatagt ttttatattt ttcatcaaaa ttacggctgg tagcgaccat ttcaggtaac
     6421 ggtgccatgc ttttgaattt tttataatgc gtcgcggcct gggccaatgt tttcctggcg
     6481 ctatcgagca attcaacttt ggcgttactt tgttgattgg aggaatccat catcatccgc
     6541 accgctgaac gactcaggtt aatgcgcgtt tgcagcatta aatcccaggt tgacgtcagc
     6601 tcgccctgct gttcccgtaa ttgattggaa accacaaagc tcttctggct atggtgaagg
     6661 gaagaaaaaa acaaactgcc ggaaataagc tgtaacagtg cgaatacccc cagcaccatt
     6721 accaacagcg tgactacgcg gatacggtta atcataaggc accttcctga aaacaagttg
     6781 atctcgttat cggcaagaag gggggaaact ttattgctga tgccacccgc cgcgaaattg
     6841 aaataaaaaa cccgatgcgc agatcatcgg gttcatttca attgaggaaa tcgggagaat
     6901 tacgccactt ctgacgccgc gctatctaac agcgccatct cttcgctgtt cagcagtttt
     6961 tcgatgttca ccagaatcaa catacggtcg cccagtgcgc ccagtccagt gagatattct
     7021 gttgaaagcg tcacggcaaa ctctggtgcc ggacgaattt gctccgccgt caatgaaagc
     7081 acgtctgaaa cgccgtcaac cacgatgccg accacccgct gtccgagatt caggacgata
     7141 actaccgtgt tgtcgttata gtccacatcc acctggctga acttaattcg taagtcaaca
     7201 atcggcacaa taacgccgcg cagattcgtg acgcctttga taaacgctgg cgtgttcgca
     7261 atccgtgtta cctgatcgta gccacggatc tcctgcactt tcaggatatc aataccgtac
     7321 tcttcatcac caagggtaaa taccagaaat tcctggcctg acggctcgct ggccagcttt
     7381 gttacattcg tcataccggt catattgtta cctttttact cattcaggcg gcggtgttcg
     7441 ccatacgttg ttcgcggttt atcgcctgca aggcggagac atcaacaatc agtgccacgc
     7501 tgccgtcgcc aagaatggtc gcagcagaaa tgccggggac tttgcgatag ttactttcaa
     7561 ggtttttaac cacaacctgg tgttgaccaa ttaattgatc caccagcaag gcgtagcggc
     7621 gaccgccact ttgtaagatc accacaattc cctgggtggc ttcggttttc gcgcccgcga
     7681 cgttgaacac tttccacagt tcgacgatgg gcagatattc accccgcact tccagcaccc
     7741 gctcgccgcc ggccagtgga tggagatcgg cttcacgggg ttgcagtgat tccataacag
     7801 cattcagcgg cagaatgaaa acttcatccg caacgcgtac ggacatgccg tcgaggatgg
     7861 ccagcgtcag cggcagtaaa atgcggatcg tagtgccagt accctgcttc gactggattt
     7921 cgacatgacc gcccatctcc tggatattac gtttaacgac gtccatgccg acgccgcgcc
     7981 cggagacgtc ggtgacctgc tctgccgtgg agaagcctgg tgcaaatatc agcattgcaa
     8041 cttcgtcgtc gctcatgttt tcgctgacag tcaaaccttg cgaggccgct tttgccagaa
     8101 ttcgctcacg gtttagcccc gccccatcgt cggtcacttc aatgcaaatg ttgccgccct
     8161 gatgttcggc agacagaatt aaatttccga cgctgttttt acctgcggcg agccgttttt
     8221 ctggcagttc aataccgtga tcgaggctat tgcgtaccag gtgggtcagc gggtcgataa
     8281 tgcgttctat caggctcttg tcgagctcgg tggaactgcc caccagcgtc agttctacct
     8341 gcttgccgag ttttcccgcc agatcacgca ccagccgggg atagcgacta aagacatatt
     8401 ccatcggcat catgcgaatc gacatcactg attcctgcaa atcacgggcg ttacgttgta
     8461 actgccccat gctggtaatc aaatcaccat gattaaccgg gtccagttcg ctggaacgct
     8521 gggcaagcat ggactgggtg ataaccagct cgccgacgag gttaattaat tgatcaacct
     8581 tttctaccgc tacacggatg ctggtggatt cactgctgcg cgtcgttttt tcccgctcca
     8641 cgcggccggt tggcgcttgt tcggctgcca gtttaagcac aggtggggta gctatttttg
     8701 gcgaaacttc tactgtttca aaggtaatct gatcggcttc aatcacaaaa cagagtaccg
     8761 ctgtgatgtc atcttcggcg atatcgcccg gtaatattgc cgagagagaa tccgcccctt
     8821 tcaccacgtc atttaacgtt gtcagatgcc ccagctcttc ttccagcagg tcgacttcgc
     8881 cggccttcag gcgcgaaagg ataattcgtc gcggcaactg actgcgactt tgctcatctt
     8941 gcggttcact tttggcaacc acacttaatc gggtcactgc ggatggcgtt tcgcctttcg
     9001 cttctaatgc cagttgacgc aaggcctggc agatataatc gtagctggcg gcatccggct
     9061 cttgcgactg tttataagcg tcgagctgtt cttgcatgat gtccttcgtt tccaaaaaca
     9121 gattgataat gtcggtgttg agttgcatct cacctcgtct ggcttcatcg agcaggtttt
     9181 ccatcagatg cgtggtttcc tgcaaaacgc tgaagccaaa agttcctgcc cctcctttga
     9241 tcgagtgggc agcccgaaag atggcattca attgttcggc atctggcgct tccggctgca
     9301 aaaccagcag atgctgctcc atatcagcca acagttcgtc cgcttcatca aaaaatgtct
     9361 gataaaaatc gcttatatcg atgctcacgc tgtcacctcg gttcggctga tggcattgtg
     9421 ggaacactga cctgtggtgc aacctcaggt ttttctaggg cgcttactgg ctcattctgg
     9481 ctttcggcgt tttcatgcaa aatggcctgt tcggcttgtt tgttcagtac caacaggctg
     9541 atgcgacggt tgacggcatc atcaggtccg cgatcgctta agcgcatcgt tgccgccatg
     9601 ccgacgacac gtaacacttt gccgctatcc aaccctccga ccatcagttc gcggcgggat
     9661 gcattggccc gatcggcaga aagctcccag ttgctgtaac ctttctcacc gctggcgtag
     9721 ggaaaatcat cggtatgacc tgaaaggcta atacggttgg gaataccgtt cagcacaggc
     9781 gcgatggcgc gcagaatgtc gcgcatatag ggttcgacat cggcactgcc ggttctaaac
     9841 atcgggcgat tctggctatc gatgatctga atacgtagac cttcctggac cagatcgatt
     9901 ttgagatgga gacgtaacgc ccgcagtttc ggatcggact ctatcaactg gtcgagatca
     9961 ccccgcaatt tccgcaatcg actttgctcc atgcgttttt tcagctcttc gatgttcggc
    10021 tgcttattca cttccccctg gctttgggtg taatcatcac caccgccggg aattgggctt
    10081 tcactattgg aaatgcgatc gccgcccgta accgctgtcg ccagtggagt ccggaagtac
    10141 tccgcaatct gaatcaactc ttttgggctg gagatggaga tcagccacat caccagaaaa
    10201 aaggccatca tcgcagtcat aaagtcggca taagcaatct tccacgatcc atgtgctgcc
    10261 ccgtggcttt tggctttgcg tcgtttgacg acaataatcg gatgcgcttg attcttcatg
    10321 cttcctcggt tgtcgtctgt tgttgcggat ttttcaccgc acgcacatgc tcttccagtt
    10381 caataaacga cggacgttcg ctggaataga gcgttttgcg accaaactca acggcgatag
    10441 gcggtgcgta accgttcaga ttagaaagca gagtgacttt gacgcactgc atcattttgc
    10501 tggtttcggc gcttttctga cgtaaaacac tcgctagtgg ggaaataaat ccgtaagcca
    10561 ataaaatacc gaggaaagtc cccaccatcg catgtgcgat aagcgccccc agctcggcgg
    10621 caggacgatc ggctgaacct aacgcgtgaa cgccccccat tacagccgcg acaataccaa
    10681 acgccggaag tgagtccccg accagcgcca gactgttcgc cgggacttct gcctcgcttt
    10741 cgtgcgtctc aatctcttca tccatcaggg cttcgatttc gaaggtgttc atgtgaccgc
    10801 tgataatcag gcgcagataa tcgacgataa aatcaagcat gacgctatcc gcgaggatgc
    10861 gtgggtagct ggcgaagatc tcgctctcac ggggattttc aatatcacgt tccagcgaaa
    10921 acatccccat ctgccgcgat ttcgccatca accgataaag cagagccagc agatccatat
    10981 acattgcttt ggtgtatttg gagcgacgaa acagcaacgg cagcgctttc agcgtgcctt
    11041 taatcgcttt gccattattg ccgacgataa acgacccaat ccctgcaccg gcaataatca
    11101 ccagttcagc gggttgatag agtgctccaa ggcttccacc ggtcatcaaa taaccgccga
    11161 aaactgtacc gagaacaacc aggtaaccta ataagataag cacgacatca tccttccact
    11221 gttgaccatg acaggatgtt cagtcgtcag gcgttaacgc gcgattgggg aaaaaaaaag
    11281 cagcggtacg tcgttaccgc tgctggaatg ttgcgccaca ccgtatcagt taaacagcct
    11341 gtactctctg ttcatccagc agttgtggga taatatcggc aggattctgg gaaagtttac
    11401 gtctttttac tgcccgggat ggcggttgac ataagctgca ggcaaagctg ccaacaggct
    11461 ggtgagcgtg ggtaataaaa ttgccgccgc agcagttgca gctggaaagt tgcagtaatc
    11521 cactttcaac aaaccgcacc aatgtccagg cacgggttaa tgccagcagt ggtccttctt
    11581 ctgcttgtgg gcactgttca aggtataaac ggtaggcttt gatcaccgca tcgacgccat
    11641 tacacaaacc ggttttcagt aaaaactgcc atgcattaca gaacatcgaa gcatgaacgt
    11701 tttgttccca ggtcataaac cagtcggttg agaatggcag catgcctttc ggcggtgggc
    11761 ttccgcgcag ttctttataa agttttatca ggcgtccgcg acttaactgt gtttcgcttt
    11821 ccagcatctg caaacgagcg cccagggtga tcaattccat tgccagctga atatcccgcg
    11881 cttcctgaac aatgcttttt tcactcatga tcaggccctt ttcttgcgca gcgcttcttc
    11941 aggctgatta acatcattca gcaagcgtgt tgagagcatg atgccggtat gaatttgctg
    12001 gagatcgtca acgcgggaat cttgcgtcaa ctgagtaatc gtctggtggc tgtcaaaacg
    12061 gaagtgacaa accagctgat tggtttctgc cagcttaacc atttgcggaa ga
//
LOCUS       DAJTRD010000106        12066 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTRD010000106 DAJTRD010000000
VERSION     DAJTRD010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.106"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            321..1961
                     /gene="trg"
                     /locus_tag="OO315_003339"
     CDS             321..1961
                     /gene="trg"
                     /locus_tag="OO315_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5892134.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(1999..2922)
                     /locus_tag="OO315_003340"
     CDS             complement(1999..2922)
                     /locus_tag="OO315_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5892135.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3139..4482
                     /locus_tag="OO315_003341"
     CDS             3139..4482
                     /locus_tag="OO315_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5892136.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4707..6362
                     /gene="opgD"
                     /locus_tag="OO315_003342"
                     /gene_synonym="mdoD"
     CDS             4707..6362
                     /gene="opgD"
                     /locus_tag="OO315_003342"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5892137.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6502..6726
                     /locus_tag="OO315_003343"
     CDS             6502..6726
                     /locus_tag="OO315_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5892138.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6789..7325
                     /gene="rimL"
                     /locus_tag="OO315_003344"
     CDS             6789..7325
                     /gene="rimL"
                     /locus_tag="OO315_003344"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5892139.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7320..8300)
                     /locus_tag="OO315_003345"
     CDS             complement(7320..8300)
                     /locus_tag="OO315_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892140.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8424..9416
                     /gene="tehA"
                     /locus_tag="OO315_003346"
     CDS             8424..9416
                     /gene="tehA"
                     /locus_tag="OO315_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5892141.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9413..10006
                     /gene="tehB"
                     /locus_tag="OO315_003347"
     CDS             9413..10006
                     /gene="tehB"
                     /locus_tag="OO315_003347"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5892142.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10309..10965
                     /locus_tag="OO315_003348"
     CDS             10309..10965
                     /locus_tag="OO315_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5892143.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(10983..11489)
                     /gene="tnpA"
                     /locus_tag="OO315_003349"
                     /pseudo
     CDS             complement(10983..11489)
                     /gene="tnpA"
                     /locus_tag="OO315_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            11497..12066
                     /locus_tag="OO315_003350"
                     /pseudo
     CDS             11497..12066
                     /locus_tag="OO315_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3122 a         2825 c         3031 g         3088 t
ORIGIN      
        1 aaatatcggt cggggccttc gtctttctga ttcccggtta gcctgaaaac agaaagtctc
       61 aggcacccgc aggcatccta tgaggtttcc ttagggacga aaataatcac ttcacgaaat
      121 tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc cagtttagcc attcgttact
      181 ctcttcattc caatagcatt aattttctct gcaataattg ttgtaaaaat gtgacgcaaa
      241 gaggtttttg gtcataagta attaccgtca agtgccgatg actttctatc aggagtaaac
      301 ctggacgaga gacaacggta atgaatacaa ctccctcaca gcgattaggt tttttgcatc
      361 acatcaggct ggttccgtta tttgcctgca ttcttggcgg tatcttagtt ctattcgcat
      421 taagttctgc tctggctggc tatttcctct ggcaggccga tcgcgatcag cgtgatgtta
      481 ctgcggagat tgagattcgg accgggttag cgaacagttc agattttttg cgttcagccc
      541 ggatcaatat gattcaggcc ggggctgcga gtcgtattgc ggaaatggaa gcaatgaagc
      601 gaaatattgc gcaagccgaa tcggagatta aacagtcgca gcaaggttat cgtgcttatc
      661 agaatcgacc ggtgaaaaca cctgctgatg aagccttcga cactgaatta aatcaacgct
      721 ttcaggctta tatcacgggt atgcaaccta tgttgaaata tgccaaaaat ggcatgtttg
      781 aagcgattat caatcatgaa agtgagcaga tccgaccgct ggataatgct tataccgata
      841 ttttgaacaa agccgttaag atacgtagca ccagagccaa ccaactggcg gaactggccc
      901 atcagcgcac ccgcctgggt gggatgttca tgattggcgc gtttgtgctt gccctggtca
      961 tgacgctgat aacatttatg gtgctacgtc ggatcgtcat tcgtccactg caacatgccg
     1021 cacaacggat tgaaaaaatc gccagtggcg atctgacgat gaatgatgaa ccggcgggtc
     1081 gtaatgaaat cggtcgctta agtcgtcatt tacagcaaat gcagcattca ctggggatga
     1141 cagtagggac tgttcgacag ggcgcggaag agatttatcg tggcaccagc gaaatttcag
     1201 ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc ggcggctatc gaacaaactg
     1261 ccgccagcat ggagcaactc actgcgacgg tgaaacagaa tgcggataac gcgcatcatg
     1321 ccagcaaact ggcgcaagag gcttctatta aagccagcga tggcgggcag acggtttccg
     1381 gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa gaaaatttct gagatcaccg
     1441 ccgtcatcaa cagtattgct ttccagacga atattctggc actgaatgct gccgttgaag
     1501 ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt cgccagcgaa gtacggacac
     1561 tcgcaagccg cagcgctcag gcagcgaaag agattgaagg cttgatcagt gaatcagtca
     1621 ggttaattga cctggggtcg gatgaggtgg caacggcagg gaaaaccatg agcactattg
     1681 ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat cgccgccgcc tcggatgaac
     1741 aaagtagagg cataactcag gttagccagg cgatttctga aatggataag gtgacgcaac
     1801 agaatgcttc tctggtagaa gaggcctcag cagcggcggt gtcccttgaa gaacaggctg
     1861 cacgattaac tgaggcggtg aacgtattcc gtctgaacaa acattctgtg tcggcagaac
     1921 ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg aaaatgttca aggagggatc
     1981 gacagatccc ttcacctttc agaacggcat tgattttcga atagcgttaa tcatcaactg
     2041 gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt ccaatcggtt cgcctgcacc
     2101 atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc aggtcgtctt ttacagcgcc
     2161 agaagggaca aaccacacgt aatcgtattc aacggtaagt tgacgagata gcgaagtaga
     2221 cagcgtttcg atacaacccg aaggaatttt acagccctgg ctctgcacta atgcatctga
     2281 atgctggcgt ggcgcagtgc cttctggtga tacaacgacc ggccattcca gcacccggct
     2341 tagcgttacg ttctcctgaa gtagcgggtg attagggcgg acaaccagct tcaacgattc
     2401 aagaaacagc agttcgtaat taagcccggt catcagttca ggatctgaca tccgaccaat
     2461 gccgatatcg atttccccgg tttttaaacc cgccagaatc atagggttac tcattgtcgc
     2521 aacttgcaag gtcgtctctt tttgttgctg atgaaactga cctataaccg aaggtaatat
     2581 ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca tcattattaa gaccttcttt
     2641 acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga actctgactg catgcgttaa
     2701 aaattgttcg ccgggtaagg taagttgcgc cccctgacga ccacgctcaa acaagcgagc
     2761 gcccgtcagc tgctccagtt cattcaatgt attagagagc gcaggttgac tcaaattaag
     2821 ggtttcagct gcgcgcccca aagttccttg ttgtgcgaca gctacgaatg tatgaaggtg
     2881 gcgcaaacgg atgcgctgac taaacagact atttttttcc ataagcgatg ttaaaaacga
     2941 agcggtgtcg ctgacaagtg aagttgtttg attatgataa cttgattgca aaatattatt
     3001 aacaattaaa gcaattatgt tacagcaaaa tggataatat tgatgttttc gcggcgagat
     3061 cacagtttgt aaattcttcc cgccagagtg aatgcggtta cctacactcc agattactga
     3121 ccactggagg cagacactat ggcgaacagc atcacggcgg atgagattcg ggaacagttt
     3181 tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc aatatggcac gctgctggaa
     3241 ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc ctcaactgca cgaaaaaatg
     3301 gtaaatgcag acgagctggc gcgactgaat gttgaacgtc atggggcgat tcgcgttggg
     3361 actgcacaag agcttgctac tcttcggcgg atgtttgcca ttatggggat gtacccggtg
     3421 agctattacg atctctcgca ggcaggggtg ccggtacatt cgacagcatt tcggcccatt
     3481 gatgatgctt ctctggcgcg taatcccttc cgcgttttta catccttact ccgccttgag
     3541 cttatcgaga acgaaatttt gcgccagaaa gcggcggaga ttctgcgtca gcgcgatatc
     3601 ttcaccccac gttgtcgaca actgttagag gaatatgagc agcagggcgg ttttaacgaa
     3661 acacaggcac aggagtttgt gcaggaagcc ctggaaacgt ttcgctggca ccagttagca
     3721 acggtagatg aagaaaccta tcgcgccttg cacaacgaac atcggttgat tgctgatgtg
     3781 gtctgttttc ctggatgcca tatcaaccac ctgacgccac gtacgctgga tattgaccgg
     3841 gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa ttctcatcga ggggccgccg
     3901 cgccgcgagg taccgatttt actacgccag accagcttta aagcactgga agagacggtg
     3961 ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct ttggtgaaat tgagcagcgt
     4021 ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg atcttctgcg taacgctgga
     4081 accgggcagg ataatctcac tcaccaaatg catttacagg aaaccttccg cacttttcct
     4141 gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt tccggtaccg tctgacgcct
     4201 tcgggtgagg cgcatcgtca ggcgattcat cctggagacg atccacagcc cttaattgaa
     4261 cgtggttggg tagtggcgca acccatcacc tatgaagatt tcttgcccgt tagcgcggcg
     4321 gggatcttcc agtcaaatct gggtaatgaa acgcagacac gcagtcacgg taatgccagt
     4381 cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg atgagttcca gctttaccag
     4441 gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt aaattctgac catccgcctt
     4501 tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat cccatcgaca taaaaaaaat
     4561 gccgatttat gcatattctc tcagttcaac aattggatta ttaataaata ttgtctagag
     4621 tgagcggtca taaataagca ctttcttgcc gctgaaaacg accagcgcgg gaccattcac
     4681 aacaccagaa ggactcactt tcaggtatgg atcgtagacg atttattaaa ggttcaatgg
     4741 ctatggccgc cgtgtgcggt accagcggca ttgcttctct tttttctcag gcagcattcg
     4801 cggcagattc tgatattgcc gacgggcaaa cccagcgttt tgacttctcc attctacagt
     4861 caatggcgca cgacttagcg caaacagcgt ggcgtggtgc gcctcgtccg ttacctgaca
     4921 cgctggcgac aatgacgccg caggcttata acagtattca atacgacgcc gaaaaatcgc
     4981 tctggcataa cgttgagaac cgtcaactgg acgctcagtt cttccatatg ggaatgggat
     5041 tccgtcgccg cgttcgtatg ttttctgtag atccagcaac acatctggcg cgtgaaattc
     5101 actttcgccc ggagttgttc aaatacaacg atgcaggtgt tgataccaaa caattagaag
     5161 ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa agcccccgaa ctggcgcgcc
     5221 gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc cgttgatgat acatatcaat
     5281 acggtttgtc ggcccgcggc ctggcgatcg acacttacac cgacagtaaa gaagagttcc
     5341 ccgactttac cgccttctgg tttgatacgg taaaaccggg ggcaactacc tttaccgttt
     5401 atgcgttgct cgatagcgcc agcattactg gtgcctataa gttcactatc cattgtgaga
     5461 aaagtcaggt gattatggat gtggaaaatc acctgtatgc gcgcaaagac attaaacagc
     5521 tgggcatttc gccgatgacc agtatgttca gctgcggtac taatgaacgt cggatgtgcg
     5581 atacaattca tccgcaaatt catgactctg atcgtctgtc catgtggcgg ggcaacggcg
     5641 agtggatttg ccgtccgctg aataatccgc aaaaattgca gttcaatgct tacaccgaca
     5701 acaacccgaa agggtttggt ttattgcaac tggatcgtga cttctcccat tatcaggaca
     5761 ttatgggctg gtataacaaa cgcccaagtc tgtgggtgga accgcgtaac aagtggggta
     5821 agggcaccat cggcctgatg gaaatcccaa caacgggcga aacgctggat aacattgtct
     5881 gcttctggca gccagaaaaa gctgtaaaag caggtgatga gtttgcattc cagtatcgtc
     5941 tgtactggag tgcgcaaccg cctgttcatt gcccattagc gcgcgttatg gcgacgcgta
     6001 ccggcatggg cggtttcccg gaaggttggg cgccaggtga acactatccc gaaaaatggg
     6061 cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa agctgccgcg ccaaaaggca
     6121 ttgagccggt gattacgctt tccagtgggg aagcgaagca aatcgaaatt ctctatattg
     6181 aacccatcga tggttatcgt attcagtttg actggtatcc gacttcggac tccactgatc
     6241 cggtcgatat gcggatgtat ctacgttgtc agggggacgc tatcagtgaa acatggctgt
     6301 atcagtattt cccgccagcg ccggataaac gtcagtatgt tgacgaccgc gtgatgagtt
     6361 aatcgttttt tcttcggcac cttcttcggg aggtgccgtc tggttaaaca cgatcccgct
     6421 cgcatttttc cctaagttaa atgagtaatc tgatggtgtg tatttcagat acaccgtgtc
     6481 agccactaac agggagtgcg tatgtttcca gaataccgag atttaatatc ccgtctgaaa
     6541 aacgaaaatc ctcgctttat gtccttgttc gataaacaca ataaacttga tcatgaaatt
     6601 gccagaaagg aaggttccga cggtcgaggg tacaatgcgg aagtggtccg catgaaaaaa
     6661 caaaagctac agttaaaaga tgagatgctc aaaatcctgc agcaggagag cgtcaaagag
     6721 gtgtaaactt tcctaagccg ccagccaggc ggctttttta acaacagcat ggattgactg
     6781 gagataagat gactgaaacg ataaaagtaa gcgaatcact tgaattacat gctgttgcgg
     6841 aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa taaaacctgg ttacagcagt
     6901 cgctaaactg gccgcagttc gttcagagtg aagaggacac gcgaaaaacg gtgcagggta
     6961 atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat gattttcaaa gaagatgaac
     7021 ttatcggcgt tatctcgttt aatcgtattg aaccactgaa taaaactgct gaaatcggct
     7081 actggctgga cgaatctcat caggggcagg ggatcatttc tcaggcgctg cagacattga
     7141 ttcatcatta cgctcagtct ggtgaactta gacgcttcgt gatcaaatgt cgggtggaca
     7201 atccgcaaag caaccaggtc gctttgcgca atggttttat ccttgaaggt tgcctgaaac
     7261 aggctgagtt cctgaatgat gcctatgatg atgtgaactt atacgcgcgt attatcgatt
     7321 cataataacc ctgccagcgg cgttcgcgta atgcgctcct cgccgttaat gacttttgga
     7381 ccacggacat gaaccgtgtc gccatcaaag gcttcaacga cggcgtggtc ggttaactcc
     7441 acatggtttt caattatcac cgcacctgta atacggctct caccttgtat tacaacgtgc
     7501 tcgtcgagca gaatcggtcc accacgtacc acggcatttc cgccaatcaa gacatgatgt
     7561 tttaacacac aattaccttc cacaatggca tattccgcca cctgcgaact gtaatgaatc
     7621 gtcggaatgg catcttcttc tatgccagct ttcacctgcg cgtgaccgta gactttagcg
     7681 caatcgcata gccagacgtt gttctcttca ttaccttcaa cactggcaaa atcaaaaact
     7741 tcggcgcgat gttcaataaa agcataccgg acgacggcat cgccataaat ttgtgcctgg
     7801 tggacaatac gcgaggcgct aacccttgcg cgatcataaa tttggagcag gagttgatgg
     7861 tcgggcgtta agccttgtgc ggcgacgatc atagaatgtt gatcaattaa ggcgtgacca
     7921 aatattctac attgcacgta aactaatgag tcatgaatgg tgacactgtc acttatatat
     7981 gcaccctggc tgatttcact attatcgatc cagacgttat ccgttgcgta aacctctccc
     8041 cacagcacgc tggtgccggt gatacgcgta ttaccggaaa tcaccgttcc gccgaacgca
     8101 atagcattct ggtcgtaaat ccagcaattt ccttcctgtg ctaacaccgt ttcgcgatcg
     8161 atccagccgc cagcggttcc tgctatcaca tcgttaaaat cgctaatggc gataatctgc
     8221 cgtaataaca cacttttttt agtgccatct tcctgataac taaaggctcg ctgttcttca
     8281 cttaggcgat atttgcgcat agcgttttcc cacaggtgac ttactataac cgtagcaaat
     8341 tctgcggctc tggctatgct cgagaaaatt ccataaaatg catttcaaat atactttata
     8401 aattaaacaa aatgagtaag aagatgcaga gcgataaagt gctcaatttg ccggcaggct
     8461 actttggtat tgtgttgggg acgataggga tgggatttgc ctggcgctat gccagccagg
     8521 tttggcaggt cagccactgg ttaggggatg ggctggtgat tctggcgatg atcatctggg
     8581 gattattgac tagcgcattt attacccgac tcatacgctt tccgcatagc gtgctggcgg
     8641 aagttcgcca tccagtgctg agcagttttg tgagtttgtt tccggcaacg acgatgctgg
     8701 tggcgattgg ttttgttccg tggtttcgcc cactggcggt gtgcatgttc agttttggtg
     8761 tcgtggttca gttggcttat gccgcctggc aaactgcggg attatggcgc ggatctcacc
     8821 ctgaagaagc taccacgcct ggactgtatc tgccgacagt tgccaacaac tttatcagcg
     8881 caatggcctg tggtgcgttg ggctacaccg acgccggtct ggtgttttta ggcgcaggcg
     8941 ttttctcatg gctaagcctt gaaccggtga tcttgcagcg tctgcgcagt tcgggagaat
     9001 tacccacggc actgcggaca tcactcggca ttcagctcgc tcctgcgctg gtggcctgta
     9061 gtgcctggct gagcgtcaac ggcggcgagg gtgacacgct ggcgaaaatg cttttcggtt
     9121 atggactgct gcaactgctg tttatgctac gtctgatgcc atggtatctc tcccagccat
     9181 ttaatgcttc attctggagt ttctcgttcg gcgtatctgc actggcaacc accggtttgc
     9241 atctggggag tggcagcgat aatggatttt tccatacgct ggcggtgccg ctgtttatct
     9301 ttaccaattt tattattgca atactgctca tccgtacttt tgcgcttctg atgcagggaa
     9361 aattgttagt cagaaccgag cgcgccgttt taatgaaagc agaggacaaa gaatgatcat
     9421 tcgtgacgaa aactatttta ctgataaata tgaattaacc cgcacacact ctgaagtact
     9481 ggaagcggtg aaagtggtta aaccgggtaa aacgctggat ctgggctgtg gcaatggtcg
     9541 taacagtctt tacctggcag ccaatggtta tgatgttgac gcatgggata aaaatgccat
     9601 gagtatcgcc aacgtcgagc gcattaaatc cattgaaaat ctggataatt tacacacccg
     9661 ggtcgttgat ctgaataacc tcacatttga tggacagtac gattttattc tttcgactgt
     9721 ggtgctgatg ttccttgagg ctaaaaccat cccagggttg attgccaata tgcaacgttg
     9781 cactaaacct ggtggttaca acctgattgt ggcggcgatg gataccgctg attatccatg
     9841 taccgtcggc ttcacgtttg ccttcaaaga gggagaatta cgtcgatatt acgaaggctg
     9901 ggagagggtg aaatacaatg aagacgtcgg cgagctgcac cgcaccgacg ccaacggtaa
     9961 tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa aaatgacccg gtaagcacaa
    10021 aacgcgtgaa aattccccac gctgagatga tttactgttc ttcttttcgg taagcatatt
    10081 ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag ttttttagca gcttaactta
    10141 ctgaaattta actactgatg attgacttag cccctttttc ggcattgact atgtcgtctg
    10201 aaaaggggct gaaaaattta ttttcaccaa cacttttttt gccacaacac gaagcggcgc
    10261 tttttgctat aacttagaaa gtaatataat catctcagga aactattcat gcgtaccaca
    10321 tcatttgcga aagttgcagc tttatgcggc ttgttggctc tgtctggttg tgcctctaaa
    10381 atcacccagc cagataaata taacaattac tctgatttaa aagaaacaac ctcagcaaca
    10441 ggtaaacctg ttttacgttg ggtagacccg agttttgatc aaagcaaata tgacagcatc
    10501 gtctggaacc caatcactta ttatccggta ccaaaacctt ctacccaggt agggcagaaa
    10561 gttctggata aaattttgaa ctataccaac accgaaatga aagaagcgat agcgcagcgt
    10621 aaaccagtgg ttaccaccgc tgggccgcgt agtctgattt tccgtggggc cattaccggt
    10681 gtagatacca gcaaagaagg gctgcaattc tatgaagtgg ttcctgttgc attagtggtt
    10741 gcggggacgc aaatggctac aggccaccgt accatggata ctcgcctcta ttttgaaggt
    10801 gagctgattg atgcagcgac taataaaccg gttatcaaag tcgttcgtca gggcgaaggt
    10861 aaagacctga ataacgaaag tacgccaatg gctttcgaaa atattaaaca agttattgat
    10921 gacatggcga ccgatgccac catgtttgac gttaacaaaa agtagttcca gacgcgccat
    10981 cgttagatgg cgctttttat ccggtgcgcc gtaaaacccc atccttcagg gcggggatat
    11041 aaggcgcggt tttccacctg accaggtgtt tgctgttgtt caatgtattg gcggatgacg
    11101 gatattggcg cacctccgca gctactggca aaatagccag gactccacaa aacgcctttg
    11161 tagtaatacc tgactgcaat atctggtcga tctcgtcgca gtaacctacc cgatacgcct
    11221 ttgaggctgt ttaccagact ggatatcgcc agtttgggag gttagttaat taacaaatgg
    11281 acgtgatctg gttcgccatc catttcaacc agttcagctt caaaataagc acatacattt
    11341 gaaaagtaag tgcgtagttt ttctgtcgcg tcatggtcaa aaatctggcg tcggtatctg
    11401 gtgacaaaga ctaggtgaac atgcatcagg aaaacacaat gccttccacg ccggatatcg
    11461 gtttcttttt tcacagacca aagtatcatt tgacctgtga aacgattaca ggcatttaaa
    11521 ttccagttaa gaccaggtgg tcaacaggag tgtgaaatga ggcgcttcgc aggcgcatgt
    11581 cgtttcgttt tcaatcgtgc tctggcacgt cagaatgaga atcatgaggc cggtaataaa
    11641 tacatccctt acgggaaaat ggcttcctgg ctggttgagt ggaaaaatgc cactgaaacg
    11701 caatggctta aagattctcc ctcacagcca ttgcaacagt cactgaaaga ccttgagcgg
    11761 gcttacaaaa acttcttccg gaagcgggct gcttttcccc gattcaaaaa gcggtgacag
    11821 aatgatgcat tccgctaccc gcagggcgtt aagctcgatc aggaaaacag ccgtattttt
    11881 ctgccgaaac tgggctggat gcgctaccgg aatagccgtc aggtcacggg tgttgtgaaa
    11941 aatgtcactg tcagccagtc ctgcggtacg tggtacatca gtattcagac agaaagtgaa
    12001 gtatccactc cggctcaccc ttcagcatca atggtcgggc tggatgctgg cgtggctaaa
    12061 ctcgcc
//
LOCUS       DAJTRD010000107        12056 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTRD010000107 DAJTRD010000000
VERSION     DAJTRD010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12056
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.107"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..285
                     /locus_tag="OO315_003351"
     CDS             <1..285
                     /locus_tag="OO315_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5892144.1"
                     /translation="AVAVWLSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWM
                     ENTIFPAMKDIPVLSGLIDTMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            296..1036
                     /locus_tag="OO315_003352"
     CDS             296..1036
                     /locus_tag="OO315_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5892145.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1095..1481
                     /locus_tag="OO315_003353"
     CDS             1095..1481
                     /locus_tag="OO315_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5892146.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1490..1819
                     /locus_tag="OO315_003354"
     CDS             1490..1819
                     /locus_tag="OO315_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5892147.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            1791..4832
                     /locus_tag="OO315_003355"
     CDS             1791..4832
                     /locus_tag="OO315_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5892148.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            4832..5161
                     /locus_tag="OO315_003356"
     CDS             4832..5161
                     /locus_tag="OO315_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5892149.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5161..5859
                     /locus_tag="OO315_003357"
     CDS             5161..5859
                     /locus_tag="OO315_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5892150.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            5864..6607
                     /locus_tag="OO315_003358"
     CDS             5864..6607
                     /locus_tag="OO315_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5892151.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6544..7146
                     /locus_tag="OO315_003359"
     CDS             6544..7146
                     /locus_tag="OO315_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5892152.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7207..10686
                     /locus_tag="OO315_003360"
     CDS             7207..10686
                     /locus_tag="OO315_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5892153.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10754..11353
                     /locus_tag="OO315_003361"
     CDS             10754..11353
                     /locus_tag="OO315_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5892154.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHRSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11417..>12056
                     /locus_tag="OO315_003362"
     CDS             11417..>12056
                     /locus_tag="OO315_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5892155.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLSE"
BASE COUNT         2835 a         2857 c         3870 g         2494 t
ORIGIN      
        1 gccgtggcgg tgtggctgag tgatgcgcag tacaccggaa aaatgacgga tgaggacgac
       61 tggcaggctg ttctgcatat tgccgttttc gtccgtgcac aggcgccgga ttctgagctg
      121 gatacgtgga tggagaacac catatttccg gccatgaagg atattccggt gctttccgga
      181 ctcatcgaca ccatgatccc actcggtttt aactatcaac gtgataatga gatggccacc
      241 tgggcgatgg cggaaatcac gtaccagatc acgtacacga attaaggagg tggtaatgac
      301 cacaccaaat ccactggcaa agacgaaagg tgcggggacg acgttctgga tgtataccgg
      361 caacggcgat gcgtttgcga accctttgtc ggacactgac tggctgcgtc ttgcgatggt
      421 gaaggatctg caacctggcg aaatgaccgc tgatgcagaa gatgacactt atctcgatga
      481 tgaagatgca gactggaaaa cgacaaccca ggggcagaaa tccgttggtg atacttcggc
      541 gacgctggcc tggcgtccgg gtgacagcgg gcagaaaaaa ctggttcagt tgttcgactc
      601 cggtgaagtc tgcgcgtttc gtatcaaata tcccaacggc actgttgatg ttttccgcgg
      661 ctggctgagc tcactgggta aaaccattgc ctcaaaagac gtgatgaccc gcactgtgaa
      721 aatcagcggt gtggggcgtc cgtatctggc agaggaaggc actgaaaccg tgggtgttac
      781 cggactgacg gtggcaccgg catctgtcag tgtcaaagcg ggagcaacca ccacactgac
      841 ctttacagta aaacctgacg gggccagtga caaagcgatc agtgtgcatt cgtcagatcc
      901 tcagactgct tcggtgaccc tgagcgggct tgtggccacg gtgaaaggcg tgaagcaggg
      961 cagtgtcagc atcgtgggca tgacctctga cggagagttt gtggcagtgg ctgcggtgac
     1021 cgtcagcgca ccataacagg acgatactca tcattgcccc ggttatccgg ggctttttgc
     1081 atccggagaa catgatgttt ctgaaacagg atacgtttaa ttatgaaaaa cagtccgtgg
     1141 tgctcagtga gctgtccggg ctgcagagaa ttgaatatct gacgtttgtt cagcagcgaa
     1201 cggcaaagtt tgatgcacag gagggagaac tgccggaggc tgaacgacag attgcttttc
     1261 tgcgtatggg aatggatatc aatgcctggc tggtttcccg ctcactgtgg aatgctgagc
     1321 agtctcagga tgttgagacg ctttgcgcat ccattatgac aacatggtcg tatgatgcgc
     1381 tgggcgcggg ggcggagagg gttctgtcgc tgagcggtat ggggaccatt gagaatgccg
     1441 gggatgatga tcatgaggcg ctgacgccgg aaaagtcctg acgcgggaaa tgcagtttgt
     1501 catgcggctt gcccgggagt tccggcgggc agactggcgg cggatgctgt cggatatgtc
     1561 ggccactgag cttggtgagt ggggcgatta tttccggatg cagagcttca gtgatgtgtg
     1621 gatggatgcg cagtttgcct cgctgaaggc attgatcgtg agaatggtgt ccggcagcag
     1681 cgatgctgcg gtggctgatt tcagcctttt accggaagag aacgggatac cggagcgaac
     1741 ggacgaagaa ctgatgcatc ttggggaagg tatttccgga ggtgtgcgtt atggaccaga
     1801 tagccaacct ggtcattgat ttggggattg atgcggcaga gtttaaaaat gaaattcccc
     1861 gtatcaaaaa ccttctgaat ggtgcagcca gcgatgcaga acggtcttct gcccgtatgc
     1921 agcgttttat ggagcgtcag actcaggcgg cccggcagac aatgcaggcg gcttcttcgg
     1981 ctgcaacagc agcatcagcc catgcgcaga cggtggagaa gaatgcacgg gctcatgaac
     2041 gcatggcccg tgaggtggaa caaacccgtc tgcgcgtgga tgccctgaat cagaaaatgc
     2101 gcgaggaaca ggcgcaggcc agggcactgg cggaggcgca ggataaagcg gctgccgcct
     2161 tttatcgcca gattgacagt gtgaaacagg ccggtgcggg gcttcaggaa ttacagcgta
     2221 ttcagcagca gatccgacag gccagaaaca gtggcggggt tggtcagcag gattatctgg
     2281 cgctgatttc ggagatcacg gcgaaaaccc gtgccctgac gcaggcagag gaacaggcca
     2341 cccggcagaa agcggcgttt atccgccagc ttaaagagca ggcaacccgc cagaatctgt
     2401 cgtcttctga gttgcttcgc gccagggcgg ctcagctggg ggtaagcagt gctgcagaag
     2461 tgtatatccg caaaatggag cgggcaggaa aagccacaca ttcgctgggg ctgaaaagtg
     2521 cggcagcccg gcgggagctg ggggtgttaa tcagtcagat ggcgcgaggc aattttggtg
     2581 cgctgagggg atccgggata acgctggcta accgtgccgg atggacaggc gcactgatgt
     2641 cgccgaaagg catgatgact ggcggcgtta ttggcggact tgtcgcggcg gtcctgggtc
     2701 tgggtaaagc ctggcatgac ggccggaagg agggcgagaa atttaaccgt cagctggcgc
     2761 tgacgggaca ttatgccggt gtcactgtcg ggcagttgtg gaaactcagc cgggccatat
     2821 ccgggaatgg tatcacgcaa catgcggcag ccggtgcgct ggcacaggtg gtgggcagtg
     2881 gggcatttca tggaaacgat attggtatgg tggcgaaagc tgccgcacag atggagcgat
     2941 cggttggcca gtcggtcagc gataccataa atcagtttaa gcggctgaag gatgatcctg
     3001 taaatgccgc gaaggcgctg gacaatgcgc tgcatttttt gaatgccact cagcttgagc
     3061 agatacgcgt ccttggggag caggggcggt ccagtgatgc ggcccggatc gccatgtctg
     3121 cgctggcaga ggaaaccggt aaacgcacat ctgatattga taataatctc aatgcgctgg
     3181 gtagtacgct gcaaaccttg tctgactggt ggaagcagtt ctgggatgcg gccatgaata
     3241 ttggtcgtga agactcgctg gatgcgcaga ttgatgcgtt acaggaaaaa attcagcgcg
     3301 cgaaaaaata tccgtggaca aacgcctcca cacaggtgga gtacgatcag cagcgtctta
     3361 acgatcttca ggagaaaaaa cgccggaagg atttgcagga tgcaaaagcg caggcagaac
     3421 ggaattacca ggagcaacag aaacgccgga atgctgaaaa tgccgcgctg aaccggatga
     3481 atgaaacgga agctgcacga catcagcggg aaattgcgcg tattaatgcc atgcagtacg
     3541 ccgaccaggc tgtcagggat gcggcgatac agcgtgaaaa tgaacgttac gaaaaagcca
     3601 ttaagaaaaa tacacgggca acccgtaatg atgaggccac ccggttattg ctgcagtaca
     3661 gtcagcagca ggcacaggtg gaagggcaga ttgctgctgc cagacagtca gcaggcattg
     3721 ccactgaaag gatgactgaa gcgcataaac agcttctggc cctgcagcag cgcatcagcg
     3781 acctggacgg gaaaaagctg acggcagatg aaaagagtgt gctggcccgt aagaatgagc
     3841 tgattcaggc gctgacgctg ctggatgtga aacagcagga gctgcagaaa cagacagcgc
     3901 ttaacgacct gagaaaaaaa acggttcagc tgaccagcca gctggcagac aaagaacgtg
     3961 cactgcgtga gcagcacaat ctggatattg ccactgcagg tatgggggat aagcagcggc
     4021 agcgctacca ggcacagttg cgcatccggc aggaataccg gcaacagttg caacagcttg
     4081 agaatgacag tcgccagaaa ggcacttacg ggacggagga ctaccggagg gctgaggagg
     4141 tgctgaaggg gagcctgaag cgacaactga atgaaaacaa acgctactgg caggaactgg
     4201 aagtggcgca gggcgactgg aaaaacggtg ccatgcgggc gtttcagaat tttacggcgg
     4261 atgcggataa tgcggcggga acggcagaac agatgtttac agtggcattc agcagtgccg
     4321 gtaatgcact ggcgacattc tgtaccaccg gtaagctgaa ttttaaatcc ttcacctctt
     4381 ccctgttgtc agatatggcc agaattatgg cacagatggc catgatgcag gcggtaaagg
     4441 gcgtcggttc tttattcggc ttcacgacta atgctgatgg cggtgtttac cagtctgctg
     4501 atttgagtcg ctacagtggc acggtggtta accgtccgac gttttttgct tttgcaaaag
     4561 gcgcgggtgt gatgggggaa gcgggacctg aagccattct gccactgcgt cgcggtgctg
     4621 atggtaagct gggggttgtg gcggatactg gtggttcagg catggtgatg tttgccccgc
     4681 agtacaacat tgagatcaat aacgacggca cgaacgggca gataggtccg gctgccctga
     4741 aggtggttta tgacctcggg aaaaaagcgg ccgcggactt tatgcaacag caggcccgtg
     4801 atggtggccg gttaagtgga gcataccggt aatggagacg tttcactgga aagtgcgccc
     4861 ggatatgaat gtggtatcag agccgaaagt ggtgacagtg aagctgggcg atggttatga
     4921 acagcgtcgt gcggcgggac tgaataacca gttgtcgact tacagcgtga cgatacgtgt
     4981 tcgtaaaggt gaacacccat ctttaaaagc ctttctggaa cggcacggtg gcgtccgcgc
     5041 atttcagtgg acgccacctt atgactggaa actgatcagg gtggtttgtc gtaaatggtc
     5101 ggcaagcgtg ggggcgttat gggtgactgt aacggccgat tttgaacagg tggtgaactg
     5161 atgcaggata tccggcagga aacactgaat gaatgcaccc gtgcggagca gtcagcccgg
     5221 gtggagctct gggaaatcga tctgacagag gtcggtggtg agcgttattt tttctgtaat
     5281 gagcagaacg aaaaaggtga gccggtcacc tggcaggggc ggcagtatca ggcatacccc
     5341 attcaggggt cggggtttga actgaacggc aggggctgtg ctgcccgtcc gacactgacg
     5401 gtctctaacc tgcacggcat ggtcaccggg atggcggaag atctgcagag tctggtcggc
     5461 ggaacggtgg tccggcgtaa ggtttacgcc cgttttctgg atgcggtgaa cttcgtcaac
     5521 ggaaacagcg acgccgatcc ggaacaggag gtgatcagcc gctggcgcat cgagcagtgc
     5581 agcgaactga gcgcggtcag tgcctctttt gtgctggcca caccaacgga gacggatggc
     5641 gcggttttcc cggggcgtat catgctggcg aatacctgta tgtggactta ccgttctgat
     5701 gagtgtggtt acacgggcag ggcagtggct gacgagttcg acaaaccaac aacggatatc
     5761 cggaaggaca aatgcagcaa gtgtatgcgc gggtgtgagt tgcgcaacaa taccggtaat
     5821 ttcggcggtt tcctttccat caataaactt tctcagtaaa tccatgacac agacagaatc
     5881 agcgattctg gcgcacgccc ggcgatgtgc gccagcggag tcgtgtggct tcgtggtgag
     5941 aacgccggag ggagacaggt atcttcccag cgagaatatc tccggtgagc cggaggaacg
     6001 gttccggatg gctccggagg actggctgcg ggcacaaatg cagggtgaga ttgtggcact
     6061 ggtccacagt catcccggtg gtctgccctg gctgagtgag gctgaccggc ggctgcaggt
     6121 gcagagtgat ttgccgtggt ggctggtctg ccgggggacg attcacaagt tccgctgtgt
     6181 gccgcatctc accgggcggc gctttgagca cggggtgacg gactgttaca cgctgttccg
     6241 ggatgcttat catctggcgg ggattgagat gccggatttt catcgcgggg atgactggtg
     6301 gcgtcacggt cagaatctct atctggataa tctggaggcc acagggctgt atcaggtgcc
     6361 gttgtcatca gcacaaccgg gcgatgtgct gctgtgctgt tttggttcat cggtgccgaa
     6421 tcatgccgcc atttactgcg gcgatggtga gctgctgcac catattcctg aacaactgag
     6481 taaacgagag aggtataccg acaaatggca gcgacgcaca cactccctct ggcgtcaccg
     6541 ggaatggcac gcatctgcct ttacggggat ttgcaacgat ttggccgccg catcgacctt
     6601 cgtgtgaaaa cgggggctga agccatccgc gcactggcca cacagctccc ggcgtttcgt
     6661 cagaaactga gtgacggctg gtatcaggtg cgcattgccg ggcgtgatgc aggtgaaacc
     6721 gaattgtctg cccgtcttaa tgagccgctg gcaaatggtg cagtgatcca catagtaccg
     6781 cgtctggtgg gagctaaaag tggcggtgtg tttcaggcgg tgctgggggc agctgttatg
     6841 gcggttgcta tatggatgcc gggggtagga attatggcga gtaatctgct gttttctctc
     6901 ggtgccagta tgacgcttgg cggtgttgca cagatgctgg caccgaaagc cagaactccc
     6961 cgtacacaga caacggataa cggcaaacag aacacctgtt tctcctcact ggataacatg
     7021 gttgcccagg gcaatgttct gcctgttctg tacggtgaaa tgcgcgtggg gtcgcgggtg
     7081 gtatctcagg agatcagcac ggcagacgaa ggggatggtg gtcaggttgt ggtgattggt
     7141 cgctgatgca aaatgtttca tgtgaaaccg cctgcgggcg gttttgtcgt ttatggagcg
     7201 tgaggaatgg gtaaaggcag cagtaagggg cataccccgc gcgaagcgaa ggacaacctg
     7261 aaatccacgc agttgctgag tgtgattgat gccatcagcg aagggccggt tgacggtccg
     7321 gtggatggat taaaaagcgt gctgctgaac ggtacgccgg tcctggatag cgaggggaag
     7381 acaaactttt ccggtgttac ggtggtgttc cgcgccggcg agcaggagca gacaccgccg
     7441 gaggggtttg aatcttccgg ctcagagact gtgctgggta cggaagtgaa atacgacacg
     7501 ccgatcaccc ggaccatcac gtcggcaaac attgatcgtc tgcgcctgac cttcggtgtg
     7561 caggcactgg tggaaaccac ctcaaagggg gaccggaatc cgtcggaagt ccgcctgctg
     7621 gttcagatac agcgtaacgg tggctgggtg acggaaaaag acatcaccat taagggcaaa
     7681 accacttcgc agtatctggc ctcggtggtg gtgggtaacc tgccgccgcg cccgtttaat
     7741 atccggatgc gcaggatgac accggacagc accacagacc agctgcagaa caaaacgctc
     7801 tggtcgtcgt acaccgaaat catcgatgtg aaacagtgct acccgaacac ggcgctggtc
     7861 ggcgtgcagg tggattcaga gcagttcggt aaccagcagg tgagtcgcaa ttatcatctt
     7921 cgcgggcgca ttctgcaggt gccgtcgaac tataacccgc agacgcggca atacagcggt
     7981 atctgggacg gaacgcttaa gccagcatac agcaacaaca tggcctggtg tctgtgggac
     8041 atgctcactc atccgcgcta cggcatgggg aaacgtcttg gtgcggcaga tgtggacaaa
     8101 tgggcgctgt atgtcatcgg ccagtactgc gaccagtcag tgccggacgg atttggcggc
     8161 acggagccgc gcatcacctg taacgcttac ctgaccacac agcgtaaggc gtgggatgtt
     8221 ctcagtgatt tctgctcggc gatgcgctgt atgccggtat ggaacgggca gacgctgacg
     8281 ttcgtgcagg accgaccgtc ggataaagtg tggacctata accgcagtaa tgtggtgatg
     8341 ccggatgatg gcgcgccgtt ccgctacagc ttcagcgccc tgaaggaccg ccataatgtc
     8401 gttgaggtga actggattga tccggacaac ggtcatgaga cggcgacaga gcttgtggag
     8461 gacacgcagg ccattgtccg ttacggtcgt aacgtcacga agatggatgc ctttggctgt
     8521 accagccggg gacaggcgca ccgcgccggg ctgtggctga ttaaaacgga actgctggaa
     8581 acgcagaccg tggacttcag cgtcggcgca gaagggcttc gccatgtacc gggtgatgtc
     8641 attgaaatct gcgatgatga ttatgccggt atcagcaccg gtggtcgcgt actggcggtg
     8701 aacagccaga cccggacgct gacgctcgac cgtgaaatca cgctgccgtc ctccggtacc
     8761 acgctgataa gcctggttga cggaaatggc aatccggtca gcgtggaggt tcagtccgtc
     8821 accgacggcg tgaaggtgaa agtgagccgg gttcctgacg gcgttgcaga atacagcgtg
     8881 tgggggctga agctgccgac gctgcgccag cgcctgttcc gctgcgtgag tatccgtgag
     8941 aacgacgacg gcacgtatgc catcaccgcc gtgcagcatg tgccggaaaa agaggccatc
     9001 gtggataacg gggcacactt tgacggtgac cggcgcggca cggtgaatgg tgtcacgccg
     9061 ccagcggtgc agcatctgac cgccgaagtc accgcagaca gcggggaata tcaggtgctg
     9121 gcgcgatggg acacgccgaa ggtggtgaag ggtgtgagct tcctgcttcg cctgaccgtg
     9181 gcagcggacg acggcagtga gcggctggtc agcacagcca ggacgacgga aaccacatac
     9241 cgcttcaggc agctggcgct ggggaattac agtctgacag tccgggcggt aaatgcccgg
     9301 gggcagcagg gcgatccggc gtcggtatcg ttccggattg cggcaccggc agcgcctgtc
     9361 actattgaac tgataccggg gtattttcag ataacggtgg tcccgaaact ggctgtatat
     9421 gacccgacgg tgcagtttga gttctggttt tcggaaaagc ggattgcgga tatcaggcag
     9481 gttgaaacca gcgcccgcta tcttggcacg gcgctgtact ggatagccgc cagtatcaat
     9541 atcaggccgg gccatgatta ttatttttac gttcgcagtg tgaacaccgt tggcaaatcg
     9601 gcatttgtgg aggccgtcgg tcgggcgagc gatgatgcgg aaggttatct gagtttttat
     9661 aaagggttga tcaataaaac gcatctcggc aaggagttgt ggacgcagat tgataacggt
     9721 cagcttgcgc cggacctgac tgaaatcagg acgtccatta cgaatgtcag caatgaaatc
     9781 acgcaaaccg tcaataaaaa actggaaaat cagagtgctg caatccagca gatacagaaa
     9841 gttcaggttg atacaaataa taacctgaac agcatgtggg ctgtgaagct gcaacagatg
     9901 aaggacggac gcctttatat tgcgggtatc ggtgccggta ttgagaatac gccagcagga
     9961 atgcagagtc aggtgctgct ggcggcagac aggattgcga tgattaatcc tgcgaatggc
    10021 aacacaaagc cgatgtttgt tggtcagggc gatcagatat tcatgaacga cgtgttcctg
    10081 aaacgcctga cggctccgac cattaccagc ggcggtaatc ctccggcatt ttccctgaca
    10141 cctggcggac ggctgacggc gaaaaatgcc gatatcagcg gtaacgtgaa cgcgaactcc
    10201 gggacgctca acaacgtcac gattaataag aactgtcggg ttctgggaaa attgtccgcg
    10261 aaccagattg aaggcgatct cgttaaaaca gtgggcaaac ctttcccacg ggactcccgg
    10321 gcaccggaga ggtggccatc agggaccatt accgtcaggg tttatgacga tcagccgttt
    10381 gatcggcaaa ttgttattcc cgcggtggcg tttcgcggtg ctaaacatga gcggaagaat
    10441 aacaatattt attcgtcatg ccgcctgata gtgaagaaaa acggtgctga aatttataac
    10501 cgaacgaccc tggataatac gctgatatat acgggtgtta ttgatatgcc tgccggtcac
    10561 ggtcacatga cgctggagtt ttctgtatcg gcatggctgg taaatggctg gtatcccaca
    10621 gcaagtatca gcgatttgct ggttgttgtg atgaagaaat ccacagcagg tatcagtatc
    10681 agctgaattt tataacccag atacgggcac cagaaatggt gcctttttta ttgcagaaaa
    10741 gcgagaggta attatgcgta aactttatgc tgccattttg tccgcagcca tctgtctggc
    10801 tgtatccggt gcgcctgcat gggcgtctga acatcggtcc acgctgagcg cggggtatct
    10861 tcatgcctcg acgaacgctc ccggcagtga tgatctgaac gggattaacg tgaaataccg
    10921 ttatgagttt acggacacgc tggggctggt gacgtcattc agctatgcca acgctgaaga
    10981 tgagcaaaaa acgcattaca gtgatacccg ctggcatgag gattccgtgc gtaaccgctg
    11041 gttcagcgtg atggcggggc tgtctgtgcg cgtgaatgaa tggttcagcg cgtatgcgat
    11101 ggcgggcgtg gcttacagcc gtgtgtcgac cttctccggg gattatctcc gcgtaactga
    11161 caacaagggg aaaacgcacg atgtgctgac cgggagtgat gacaaccgcc acagcaacac
    11221 gtctctggcg tggggggctg gcgtgcagtt taacccgacc gaatccgtgg ccattgacct
    11281 tgcttatgaa ggttccggca gtggcgactg gcgcactgac ggtttcatcg tgggtgtcgg
    11341 ttataagttc tgattagcca ggtaacacag tgttatgaca gcccgctggt tcaggcgggc
    11401 tttttgtggg gtgaatatgg cagtaaagat ttcaggtgta ctgaaagatg gggcgggtaa
    11461 acctgtcgta aattgtgcga ttgaactgcg ggccagaaga accagtccga ccgttgtggc
    11521 acacgttgtt gccacttgcg tgacggacaa taacggtgct tatgtgattg aggctgagcc
    11581 ggggtattac gaggttgcgc ttcactgtaa cggctggcag ccaacccgtg tcggggatat
    11641 tgatgtggca ccgactgatg caccggggac actgaacgcg tttctgaatg caccaaagga
    11701 tggtgattta cgtccggagg tgatgaagcg ctttgaggaa atggtggcgc aggcgcagca
    11761 gagtgccggg gctgcagccg gaaacgcaca gcagacggcg caggatgtgg cggcagccgc
    11821 aacggcccgt gatgatgcac aacgttttgc ggagaaagcc cgacaggatg caaccgtcac
    11881 agctgaggac agaaaggcca ctgcggaaga tgtgacaagc acaggagcaa atgcagccgc
    11941 agccggacag agcgcacagg atgccgcagg ttatgcccgc gcagcagaac aggccaaaaa
    12001 tgacattgat gctgcgctga ccggcactct gaaaatggct aaccatctgt cagaaa
//
LOCUS       DAJTRD010000108        11926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTRD010000108 DAJTRD010000000
VERSION     DAJTRD010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.108"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..2342)
                     /locus_tag="OO315_003363"
     CDS             complement(<1..2342)
                     /locus_tag="OO315_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5892156.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLGIRVKTDKTHYQPGELVNVE"
     gene            complement(2276..2899)
                     /locus_tag="OO315_003364"
     CDS             complement(2276..2899)
                     /locus_tag="OO315_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5892157.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="OO315_003365"
                     /pseudo
     CDS             complement(2896..4584)
                     /locus_tag="OO315_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO315_003366"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO315_003366"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5892158.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="OO315_003367"
     CDS             7507..8229
                     /locus_tag="OO315_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5892159.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..10291)
                     /gene="yfaL"
                     /locus_tag="OO315_003368"
     CDS             complement(8357..10291)
                     /gene="yfaL"
                     /locus_tag="OO315_003368"
                     /inference="COORDINATES: protein motif:HMM:NF007285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HCR5892160.1"
                     /translation="MMEITANVDTRSTTTEHGRDIEMRADGEVAVDAGVDTQWGALMA
                     DSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDVADIFPYASSLWVGD
                     GATFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNASLFNGDGTLVNATD
                     GVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAGDTLTVNGDYTGGGT
                     LLFDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTGIKVVDFAADPTQFQ
                     NNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPEPDPTPDPDPTPDPDP
                     TPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDGQTLNLRVIGGRYHY
                     TAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGGYSDNQGDSRSNMTGTRADN
                     QNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDVSEHEDGVDRYHSSGIIASLE
                     AGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDDIQTRLGLHSEWRTA
                     VGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKLRGEIKVGVTGNISQRVSLRGSVAW
                     QKGSDDFAQTAGFLSMTVKW"
     gene            complement(10301..11896)
                     /locus_tag="OO315_003369"
     CDS             complement(10301..11896)
                     /locus_tag="OO315_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AidA family protein"
                     /protein_id="HCR5892161.1"
                     /translation="MTNNASGGAVFLQQGAEFSLLPENETGMTLFANNTVTGEYNNGG
                     AIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNAINNIANDGKGG
                     AIYTINNDVYLSDDVFNNNQAYTSTSYSDGDGGAIDVTDNNSDSKHPSGYTIINNTAF
                     TNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSAAGYGDGPSTAAGG
                     FMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFTG
                     EMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDQYQNQAELNVGSTQQTF
                     VHALTGFQNGTLNIDAGGNVTVNQGSFAGIIEGAGQLTIAQNGSYVLAGAQSMALTGD
                     IVVDDGAVLSLEGDAADLTALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSGS
                     SGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASGT
                     LMVSDNSQLGDTHYNRQVIFTDK"
BASE COUNT         2757 a         3310 c         2945 g         2914 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 ccaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagacct tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcttcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctgatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgtg ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg tcataaaccg ccgagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccagcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcgcccgcta aacaagccgc
     9061 cgctaagctg caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga gggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccgtcgcgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctttg ccatcattag cgatattatt gatggcgtta gtgacacgta
    11641 aatcgacggc accagtatca ttagtaccag aagaatagat tgcgccacca tagccgcctg
    11701 cgacgttacc ggaaaaaata acatcagtaa gattcagcgt tgagttttct ttagcaaata
    11761 tggccccgcc gttattatat tctcctgtaa cggtgttatt ggcaaacaga gtcattccag
    11821 tttcattttc tggtaatagt gaaaattccg ctccttgttg caaaaatacg gccccaccgc
    11881 tggcattatt ggtcatatcc gaaaaaacca gccattgccc atcggc
//
LOCUS       DAJTRD010000109        11786 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTRD010000109 DAJTRD010000000
VERSION     DAJTRD010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11786
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.109"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            11..487
                     /locus_tag="OO315_003370"
     CDS             11..487
                     /locus_tag="OO315_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5892162.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            550..771
                     /locus_tag="OO315_003371"
     CDS             550..771
                     /locus_tag="OO315_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5892163.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            934..1308
                     /locus_tag="OO315_003372"
     CDS             934..1308
                     /locus_tag="OO315_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5892164.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            1355..1729
                     /locus_tag="OO315_003373"
     CDS             1355..1729
                     /locus_tag="OO315_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5892165.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            1726..2217
                     /locus_tag="OO315_003374"
     CDS             1726..2217
                     /locus_tag="OO315_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892166.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            2229..2426
                     /locus_tag="OO315_003375"
     CDS             2229..2426
                     /locus_tag="OO315_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892167.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2511..3071
                     /locus_tag="OO315_003376"
                     /pseudo
     CDS             2511..3071
                     /locus_tag="OO315_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            3143..4147
                     /locus_tag="OO315_003377"
     CDS             3143..4147
                     /locus_tag="OO315_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5892168.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            4429..4710
                     /locus_tag="OO315_003378"
                     /pseudo
     CDS             4429..4710
                     /locus_tag="OO315_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(4860..4947)
                     /locus_tag="OO315_003379"
     tRNA            complement(4860..4947)
                     /locus_tag="OO315_003379"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4911..4913),aa:Ser,seq:gga)
     gene            5182..6120
                     /gene="ghrA"
                     /locus_tag="OO315_003380"
                     /pseudo
     CDS             5182..6120
                     /gene="ghrA"
                     /locus_tag="OO315_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6175..6912
                     /gene="ycdX"
                     /locus_tag="OO315_003381"
     CDS             6175..6912
                     /gene="ycdX"
                     /locus_tag="OO315_003381"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5892169.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            6936..7490
                     /gene="ycdY"
                     /locus_tag="OO315_003382"
     CDS             6936..7490
                     /gene="ycdY"
                     /locus_tag="OO315_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5892170.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            7592..8083
                     /locus_tag="OO315_003383"
     CDS             7592..8083
                     /locus_tag="OO315_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5892171.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(8216..8922)
                     /locus_tag="OO315_003384"
                     /pseudo
     CDS             complement(8216..8922)
                     /locus_tag="OO315_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(8949..9365)
                     /gene="csgF"
                     /locus_tag="OO315_003385"
     CDS             complement(8949..9365)
                     /gene="csgF"
                     /locus_tag="OO315_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5892172.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(9390..9779)
                     /gene="csgE"
                     /locus_tag="OO315_003386"
     CDS             complement(9390..9779)
                     /gene="csgE"
                     /locus_tag="OO315_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5892173.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            complement(9784..10434)
                     /gene="csgD"
                     /locus_tag="OO315_003387"
     CDS             complement(9784..10434)
                     /gene="csgD"
                     /locus_tag="OO315_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5892174.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            11187..11642
                     /gene="csgB"
                     /locus_tag="OO315_003388"
     CDS             11187..11642
                     /gene="csgB"
                     /locus_tag="OO315_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5892175.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            11683..>11786
                     /locus_tag="OO315_003389"
     CDS             11683..>11786
                     /locus_tag="OO315_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5892176.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
BASE COUNT         3103 a         2760 c         2856 g         3067 t
ORIGIN      
        1 ggcagggaca atgcagcagc tttcctttct gcccggaaac atgacgcccg gcgagcgcag
       61 tctcattctg cgggccctgc aaaccctgga ccgccatctt catgaacccg gcgtggcctt
      121 cacctccacc cgtgcggcac gggaatggct gattctgaac atggcgggac tggagcggga
      181 agagttccgg gtgctgtatc tgaacaacca gaatcagctg attgccggtg aaaccctctt
      241 caccggcacc atcaaccgta cggaagttca tccccgggaa gtgattaaac gcgccctgta
      301 ccacaatgcc gctgccgtgg tgctggcgca caatcacccg tccggtgaag tcacacccag
      361 taaggcagac cggcttatca ccgaacgtct ggtacaggca ctgggcctgg tggatatccg
      421 ggtgccggac catctgatag tcggtggcaa ccaggttttc tcctttgccg aacatggtct
      481 gctttaaccc gtcacaacca catcacacct gttttcactt ttatcttctg tcttcagagg
      541 tatcacatca tgagaatcat cacccgtggt gaagccatgc gtattcacca acagcatcct
      601 gcatcccgtc tttttccgtt ctgtaccggt aagtatcgct ggcacggcag tgctgaagcg
      661 tatactggtc gtgaagtgca ggatattccc ggcgttcttg ccgtgtttgc agaacgccgt
      721 aaggacagtt ttggcccgta tgtccggctg atgagtgtca ccctgaactg atgttccgtc
      781 acccgggaaa cgagtacgac cagatgacct cagacgccgc tgtggatgag gccattctca
      841 tcaatgcata catcttcacc gaaaccggtc agcgcgccgg ctgacctgtc atcgttaaac
      901 cattcttaac cacttttcag agaggatttt atcgtgtcag acaaactctc cgggataact
      961 catcccgatg acaatcacga ccgcccctgg tgggggcttc cctgcacagt gaggccctgt
     1021 tttggcgctc gtctggtgca agagggtaac cgcctgtatt accttgccga ccgcgccggt
     1081 atcagaggcc gcttcagcga cgcagatgca taccatctgg accaggcctt tccgctgctg
     1141 atgaaacaac tggaactcat gctcaccagc ggtgaactga atccccgata tcagcatacc
     1201 gtcacgctgt atgcaaaagg gctgacctgc gaagccgaca ccctcggctc ctgtggttac
     1261 gtttatctgg ctgtttatcc gacaccagca cccgcaacca cctcataacc ccgaagctct
     1321 cacctttagc actaccagtt acagagaact taagatgaaa acattatccg acacacatgt
     1381 acgggaggta tctcgctgcc cgtctcccgt caccatctgg cagacactgc tcatccgact
     1441 gctggaccag cattatggcc tcacactgaa tgacacaccg tttgtcgatg aacgtgtgat
     1501 tgagcagcat attgaggcag gcatttcact gtgtgatgca gttaattttc tcgtggaaaa
     1561 atacgcgctg gtgcgtaccg accagccggg attcagcacc tgtccccgct ctcagttaat
     1621 aaacagtatt gatatcctcc gggctcgccg ggcgaccggc ctgatgaccc gcgacaatta
     1681 cagaacggta aataacatta ccctgggtaa gtatccggag gcgaaatgat gaaactggcc
     1741 ctgacactgg aagccgacag cgttaacgta caggcactga acatagggcg cattgtcgtt
     1801 gacgtcgatg gtattgagct cgctgaactg attaacatgg tctgcgataa cggctactcc
     1861 cttcgtgttg ttgatgaatc tgaccagacc tcagcagaat gcacgccacc atttgctacc
     1921 cttaccggca tacgctgcag taccgcacat atcacggaaa cggacaacgc ctggttgtac
     1981 tcgctgtcac accagaccag tgacttcggt gaatcagaat ggattcattt tacaggtagc
     2041 ggatatctgt tacgtaccga tgcgtggtca tacccggttc tgcggcttaa acgcctgggg
     2101 ctgtcaaaaa cgttccgtcg tctggtcgtc acactcatcc ggcgttatgg cgtcagtctc
     2161 attcatctgg atgccagcgc tgaatgcctg ccgggtttac ccactttcga ctggtaacca
     2221 ggaacaacat gaaatcatta accacggaaa cagcgctgga tattctgatt gcgtggctgc
     2281 aggacaatat cgactgcgga tcgggaatta tctttgacaa cgatgaggat aaaacggatt
     2341 cagcagcact gttgccctgt atcgaacagg cccgggagga tgtccgtacc ctgcgtcatc
     2401 tgcagcttct gcaccagaac cggtgaagct cactcaccag acttcattcc tctgacgcca
     2461 gcctgcacag ggctggcgtt ttcatttatc tgcaaaaagg aatatcgatt atgtcagata
     2521 tcacaatctc ccgtccggaa gtggtcaacg ggcatacgga cgttatctgc tcaaccagca
     2581 ttcgccacat tctggctgta cgaaagagta cactgttgca aatcgacaca cttatccgac
     2641 aactggctga aatctcggtg ctgacggaaa gcatcggagg taaaactgcc ccggactggg
     2701 ccatgaaaca ggattttcgc tgcggctgct ggctgatgga gaaacccgaa acagcaatga
     2761 aagccattac ccggaatctt gatcgcgaaa tctggcgtga cctgatgcaa cgttccggga
     2821 tgctgtcatt aatggatacg caggcccgtg atacatggta ccggtcactg gagtacgata
     2881 attttccgga aatcagcgaa gcgaacattc tgagcacatt tgaacaactg caccagaata
     2941 aggatgaggt gtttgagcga ggggtgatca acgtcttcag agggctgaac tggaattaca
     3001 aaaccaattg cccctgtaaa tttggcagta aaattatcgt caacaatctg gtgagatggg
     3061 accggtgggg ataatgatat ggtcaccccc accttaacag cagtacgctg ttaaggtaaa
     3121 tccctttaac ggagagacca tcatgcagaa catcacactt attggtatcg atctcggaaa
     3181 gcactccttc cacattcatt gtcaagacaa gtctggtaag gctcttctgc gtaaaaaatt
     3241 tacccgtact aaactgatgc agtttctggc gacatgtccg ccctcagttg ttgtaatgga
     3301 agcctgtgcg ggtgcacact ttatggctcg ctgtatctct gattttgggc atgaaactaa
     3361 gctgatttct ccacagttcg ttcgcccgtt tgtcaaaagt aataagaatg attttgttga
     3421 tgcagaagcc atatgcgaag cggcttcacg accatcaatg cgttttgtcc agccaagaac
     3481 tgagacccag caggccatga gggcgttaca ccgggtcaga gaatcgctca tcagagataa
     3541 ggtgaaaaca accaaccaga tccacggttt cttgctggaa tttggaatca gcctgccgat
     3601 aggtgatgct gtaataaaac ggttgtctct cgtgttggct gaacacgaaa tccccgaata
     3661 tctgagccgc cttttggtga ggttgcatac ccactacttt taccttgttg agcaaatagc
     3721 agagctggaa tcagaattaa gtcagtcaat caacgctgat gataccgctc agcgcattat
     3781 gacaattcct ggtgttggtc cgattactgc cagcttacta tcgtcgcaac ttggtgacgg
     3841 gaaacagttc tcatgcagca gggattttgc agcttccaca ggacttgttc cccggcagta
     3901 tagcactgga ggaaaatcga ctctgttggg tatcagtaag cgaggtgata aaaatttacg
     3961 ccggttactg gttcagtgtg cccgctcatt cataatgcga cttgaacgca agcatggaaa
     4021 acttgctgag tgggttcggg agcaactcaa caagaagcat tcgaatgtgg ttacctgcgc
     4081 tctggcaaat aaactggctc gaattgcctg ggcaatcact actcagaaaa aagaatatca
     4141 ggcctgatat agatgctgat ataactcaat tagtttattt taaacaataa gatacaagtt
     4201 acctttctgg ttttgcgaag actgattatt gatgacatga acggcaaatc gacctgatta
     4261 aaaacctaac agatgaaatg gctcactgaa gccgagggga tttttaggat tatcaggtgc
     4321 ggaactcatc ggggcgcggg catccttgcc cacagagacg ccggatagat ttaagcaagc
     4381 caaccattaa tcaaaaatca gtgttgcaaa aacgggggtg accatagatt tcatcttatc
     4441 accggacaac aggctgaccg actcgccgat ctggaaagaa tgttgcacct gttcagcggt
     4501 aaaccaatcc ccgacaaccg gggaaacatc acccttcgtc tggatgatca cattcagtct
     4561 gttcagggta aagagagcta tgaagatgag atgttcagca tcagatactt taagaagggc
     4621 tccgcgcata tcacgttcag aaaaccagaa ctggttgaca gactgaatga tattatcgca
     4681 aaacactatc ctgatatgct ggcagtatag ctttgccagt atgctcaaag atcggaaatt
     4741 gtccgataag atatgatcgc ggggcatata aaccggtttc agatggcata cccgatggca
     4801 tactgtctgg aaggaaaaat aaaaaagcgc atgttaaaca ataacatgca cttataatct
     4861 ggcggtgagg gggggattcg aacccccgat acgttgccgt atacacactt tccaggcgtg
     4921 ctccttcagc cactcggaca cctcaccaaa ttgtcgttcc tgtcttgctg gaacgggcgc
     4981 taatttaggg aaatcatgac ctgaggtcaa caaacttttt gaaaaaatcg cgcgtttatt
     5041 caaacattca atcaatgtgt ggttttaata agcgaaatct gctttttttg ccaccgacca
     5101 cggatttgtt atgctggtgg cctttgtaga tcataacgat aagtgcgaat aaatttcgca
     5161 caacgctttt cgggagtcag tatggatatc atcttttatc acccaacgtt cgatacccaa
     5221 tggtggattg aggcactgcg caaagctatt cctcaggcaa gagtcagggc atggaaaagc
     5281 ggagataatg actctgctga ttatgtttta gtctggcatc ctcctgttga aatgctggca
     5341 gggcgcgatc ttaaagcggt gttcgcactc ggggccggtg ttgattctat tttgagcaag
     5401 ctacaggcac accctgaaat gctgaaccct tctgttccac tttttcgcct ggaagatacc
     5461 ggtatgggcg agcaaatgca ggaatatgct gtcagtcagg tgctgcattg gtttcgacgt
     5521 tttgacgatt atcgcatcca gcaaaatagt tcgcattggc aaccgctgcc tgaatatcat
     5581 cgggaagatt ttaccatcgg cattttgggc gcaggcgtac tgggctgtaa agttgctcag
     5641 agtctgcaaa cctagcgctt tccgctgcgt tgctggagtc gaacccgtaa atcgtggcct
     5701 ggcgtgcaaa gctttgccgg acgggaagaa ctgtctgcat ttctgagcca atgtcgggta
     5761 ttgattaatt tgttaccgaa tacccctgaa accgtcggca ttattaatca acaattactc
     5821 gaaaaattac ctgatggcgc gtatctcctc aacctggcgc gtggtgttca tgttgtggaa
     5881 gatgacctgc tcgcggcgct ggatagcggg aaagttaaag gcgcaatgct ggatgttttt
     5941 aatcgtgaac ccttaccgcc tgaaagtccg ctctggcaac atccacgcgt gacgataaca
     6001 ccacatgtcg ccgcgattac ccgtcccgct gaagctgtgg agtacatttc tcgcactatt
     6061 gcccagctcg aaaaagggga gagggtctgc gggcaagtcg accgcgcacg tggctactaa
     6121 taaagcatca ggattcctgc tatccttggc gggaattgaa tacaggagag agttatgtat
     6181 cccgtcgacc ttcatatgca taccgttgcc agcacacatg catatagcac attgagtgat
     6241 tacattgccc aggccaaaca aaagggcatt aaactttttg cgatcaccga tcatggcccg
     6301 gatatggaag atgcgccgca tcactggcac ttcattaaca tgcgtatctg gccgcgagtg
     6361 gttgatgggg tagggatcct gcgcggcatt gaagctaaca ttaaaaatgt tgatggtgaa
     6421 attgactgca gcggtaaaat gtttgactcg ctggatctga ttattgccgg ttttcatgag
     6481 ccggtttttg cgccacatga caaagcgacc aatacacaag cgatgatctc cactatcgcc
     6541 agcggcaatg tgcatataat aagccatccc ggaaatccga aatatgaaat agatgttaaa
     6601 gccgttgctg aagcagccgc gaaacatcag gtggcgctgg aaatcaataa ttcctcattt
     6661 ttacactcac gtaagggcag tgaagacaac tgtcgtgcgg tagctgcagc ggtacgtgat
     6721 gctggaggtt gggtggcatt aggctcggat tctcacaccg cgtttaccat gggggaattt
     6781 gaagagtgtc ttaaaatcct cgacgcggta gattttccgc tagagcgcat tttgaatgtt
     6841 tctccgcgcc gcttactgaa cttccttgaa tctcgcggta tggcaccgat tgcggaattt
     6901 gcagaccttt aattactagt aatggaaata tattaatgaa cgagttttct atcctctgtc
     6961 gtgtgctggg ttcgctctat taccgccaac cgcaagatcc tttactggtg ccgctgttta
     7021 ctctgattcg tgaggggaaa ctggctgcga actggccact ggagcaggat gagttactga
     7081 cgcgtttaca gaaaagctgt gatatgacgc aagtctctgc cgattacaat gcgttgttta
     7141 tcggcgatga atgtgccgtg ccgccatatc gtagcgcatg ggttgaggac gcaacggaag
     7201 cggaagtgcg cgcttttctt tccgaacgag ggatgctact ggcggatacg ccagccgatc
     7261 acatcggcac attgctgctt gcggcttcct ggctggaaga tcagtcaacg gaagatgaga
     7321 gcgaagcact ggaaacactg ttcagtgagt atctgttacc ctggtgtggt gcgttcctcg
     7381 gcaaagtgga ggcccatgca accacgcctt tctggcgcac catggcaccg ctaacccgtg
     7441 atgccattag tgcaatgtgg gacgagctgg aagaagattc tgaagagtaa atgtgatcat
     7501 cgtcactcat aactgcaaca gataatttgt tgttgcataa aatgtgtgct cgatctcatt
     7561 catggccgcg ttttctgcta tcatgcgcgg catgaacata cttctctcta ttgcaatcac
     7621 aacaggcatt ctctccggta tctggggatg ggtggctgtt tccctgggct tactaagctg
     7681 ggcgggcttc ctgggctgta cggcctactt tgcttgcccg caaggtgggc tgaaagggct
     7741 ggcgatctcc gctgcaaccc tgcttagtgg cgtggtgtgg gcgatggtca ttatttacgg
     7801 tagtgcactg gcaccacatc tggaaattct cggttatgtc ataaccggta ttgtcgcgtt
     7861 tctgatgtgt attcaggcca aacagctgct gctttcattt gttccgggga cgtttatagg
     7921 cgcatgcgcg acgtttgccg ggcagggtga ctggaaactg gtgttacctt cgctggcgct
     7981 tgggctggta tttggttacg caatgaaaaa cagcggcctg tggctggcgg cgcgtagtgc
     8041 aaagacggct caccgtgagc aggaaatcaa aaataaagcg tgaggggcac tcacgctttc
     8101 gcttaaacag taaatgccgg attattaatt ccggcatttt tatctgtcag gattccggtg
     8161 gaaccgacat atggcggtat ttcaccagaa tgtcattctg ccgttctgct ttattttaca
     8221 aatcccacag accacggtcg ataccatcat taatcaggaa aatgacccct gtttcgatag
     8281 ccgacatcag acacagcata acgggttcgt ttgaggtgta acccacttcc ccttccagca
     8341 agcgctggta gtcaataaag cggaaaaccc cggcctgaac ttcataggaa agtatcgtct
     8401 tactggtgtt caccgaggag aggatctcac cggtactcac attgacgaca cgcaggttca
     8461 cggcaatctg atcgagctgg tattgcgtgt ctgcaccgat cccaaaatat cttgccccaa
     8521 caccgcccga tttaacgttg ctttcataac cgataatcga accttcaacc ataatatttg
     8581 ccgccgttaa agattgcagc gggattcggt tattaatggc aaccgtgccg ttttcttgtg
     8641 ccgcacgaat aaccttgcgc tcgttaagca ggttttgtaa gccctggcgc tttgcggaac
     8701 agcagtggag aagttacttg ccgggtaggg tttaaattgc ccggtttcgt cctgaatgtt
     8761 gtataccgaa acaaagatct tacccgtcgg cgctggcaga tgggtcaaat ctttgtagct
     8821 ctgagcacga ggcattaatg tcggtctggc ggcttcttta ggcggggcgg ttaagcatcc
     8881 gctcagtaac atgacggcaa ccaaaagaaa taagcgctgc atgattattt tccttatgaa
     8941 gctggggctt aaaaatcggt tgagttattt tgtaaacccg aaacctggat ggtcgaggtt
     9001 tgtccggttt tacgatctgt cacgttcaac tgcaattgac catcgcggtt agcaatatca
     9061 acaatataat cgttggtcac catgcggccc ggtttaccgg tattaatatt cgacagtagc
     9121 ccacctaaaa tttgtgactg gatggcctga gtaaagttat ctaacgctga gggtgtttca
     9181 ataccaaagt catcgttata gctcggatct ttataagagt tttgggcctg agcgctattt
     9241 aataaaaaag cgccattatt tgggttacca ccaaagtttg gattacggaa ctggaaagtc
     9301 atggttccag cccaacttaa tggcgaaata agcatgagta gaactactgc atgtttgaca
     9361 cgcattgcag cctccgaaca attttttatt tagaattcat catgcgccaa atcgtccgta
     9421 cttaataacg cctgatttat ctggcgacga tttagtgctt cttcagtttg aatcagtgca
     9481 aagacgacag ttttctcgga gtctcttttc aacgggaata aaaaagtctg gaaaataaca
     9541 tcctgattga ccgttattgt gatccagctt ccccatcgtg cactgggcct ttcattaatc
     9601 gttaagttac ccgtatagtc actttcccat ttatcactaa aggctcggta aaaatcatgg
     9661 ccaatagatg aaacagtatg gtcagttaac aatcccggga cttctacctc aacggcgtga
     9721 agattccctg cggcgaacag aaattctgcc gccacaatcc agcgtaaata acgtttcatg
     9781 gctttatcgc ctgaggttat cgtttgccca ggaaaccgct tgtgtccggt tttttacggc
     9841 tatcttcttg aaaagattat aaagatgcgt tttaaccgta ttttcgctga tgaacaacga
     9901 acgagcgatc tcgttattag acgcgccgat acgcagctta ttcaggatct ctttttcccg
     9961 atgagtaagg agggctgatt ccgtgctgtt ataacgatag ttaccagaat gtgtaatcag
    10021 gtaactggca agcttttgcg taaagtagca ttcgccacgc aggacgcctt gcaacccatt
    10081 gacaacacgt tcttgatcct ccatggcata aaaaacgccg ttgatatgag gccagttttc
    10141 aatgtcgcgg tacgggtaat cttcaggcgt atttagcaac aatattttga tattgttgtt
    10201 tttcctgctc aaagtatcct gccaataatg gataagcttt ttatccgctt ccatcatatc
    10261 cagaagaata atagagcctg aagagatatc gtccagagaa cgttgaatat tatgtaattt
    10321 tcctgtaatt gctagcgatt gtttaaggtg ctgcaagaga gctgtcgcct gcaaagaagg
    10381 tttagtgatc aacaataatg tatgaccatg aatactatgg acttcattaa acatgatgaa
    10441 accccacttt tttattgatc gcacacctga cagctgcctc taaaatagaa gcaccagaag
    10501 tactgacaga tgttgcactg ctgtgtgtag taataaatca gccctaaatg ggtaaaatat
    10561 aaaactaatg gattacatct gatttcaatc tagccattac aaatcttaaa tcaagtgtta
    10621 aacatgtaac aaaatgtaac tcattatatt aaaatgttaa cattaaggtt ttattaagtt
    10681 tagaaatgat agaaaagttg tacatttggt ttttattgca caattttaaa aaatcataca
    10741 aatggtgcta acttactaat aatacatata aaaaatattt cggtgtagtc ctttcgtcat
    10801 gtaaaacgtt tttgtttttt ctccacacct ccgtggacaa ttttttactg caaaaagacg
    10861 aggtttgtca cggcttgtgc gcaagacata tcgcagcaat cagcgacggg caagaagaat
    10921 gactgtctgg tgatttttga tagcggaaaa cggagattta agagaaaaca aaatattttt
    10981 tgcgtagata acagcgtatt tacgcgggtt ttaatacttt ggtatgaaca aaaaaagaaa
    11041 aatacaacgc gcgggtgagt tattaaaaat atttccgcag acatactttc catcgtaacg
    11101 cagcgttaac aaaatacagg ttgcgttaac aaccaagttg aaatgattta atttcttaaa
    11161 tgtacgacca ggtccagggt gacaacatga aaaacaaatt gttatttatg atgttaacaa
    11221 tactgggtgc gcctgggatt gcagccgcag caggttatga tttagctaat tcagaatata
    11281 acttcgcggt aaatgaattg agtaagtctt catttaatca ggcagccata attggtcagg
    11341 ctgggactaa taatagtgct cagttacggc agggaggctc aaaacttttg gcggttgttg
    11401 cgcaagaagg tagtagcaat cgggcaaaga ttgaccagac aggagattat aaccttgcat
    11461 atattgatca ggcgggcagt gccaacgatg ccagtatttc gcaaggtgct tatggtaata
    11521 ctgcgatgat tatccagaaa ggttctggta ataaagcaaa tattacacag tatggtactc
    11581 aaaaaacggc agttgtagtg cagagacagt cgcaaatggc aattcgcgtg acacaacgtt
    11641 aattaccatt cgacttttaa atcaatccga tgggggtttt acatgaaact tttaaaagta
    11701 gcagcaattg cagcaatcgt attctccggt agcgctctgg caggtgttgt tcctcagtac
    11761 ggcggcggcg gtaaccacgg tggtgg
//
LOCUS       DAJTRD010000110        11714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTRD010000110 DAJTRD010000000
VERSION     DAJTRD010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.110"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            126..995
                     /locus_tag="OO315_003390"
     CDS             126..995
                     /locus_tag="OO315_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5892177.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1198..1551)
                     /locus_tag="OO315_003391"
     CDS             complement(1198..1551)
                     /locus_tag="OO315_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5892178.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1689..3335)
                     /gene="groL"
                     /locus_tag="OO315_003392"
     CDS             complement(1689..3335)
                     /gene="groL"
                     /locus_tag="OO315_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5892179.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3379..3672)
                     /locus_tag="OO315_003393"
     CDS             complement(3379..3672)
                     /locus_tag="OO315_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5892180.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3948..5204
                     /gene="yjeH"
                     /locus_tag="OO315_003394"
     CDS             3948..5204
                     /gene="yjeH"
                     /locus_tag="OO315_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5892181.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="OO315_003395"
     CDS             complement(5220..5696)
                     /gene="fxsA"
                     /locus_tag="OO315_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5892182.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            6033..7469
                     /gene="aspA"
                     /locus_tag="OO315_003396"
     CDS             6033..7469
                     /gene="aspA"
                     /locus_tag="OO315_003396"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5892183.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7587..8888
                     /gene="dcuA"
                     /locus_tag="OO315_003397"
     CDS             7587..8888
                     /gene="dcuA"
                     /locus_tag="OO315_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5892184.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            9004..9342
                     /gene="cutA"
                     /locus_tag="OO315_003398"
     CDS             9004..9342
                     /gene="cutA"
                     /locus_tag="OO315_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5892185.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9318..11015
                     /gene="dsbD"
                     /locus_tag="OO315_003399"
     CDS             9318..11015
                     /gene="dsbD"
                     /locus_tag="OO315_003399"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5892186.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            11052..11627
                     /locus_tag="OO315_003400"
     CDS             11052..11627
                     /locus_tag="OO315_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5892187.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2814 a         3024 c         2881 g         2995 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttgg tcatgccgga tgcggcgtaa
     1081 acgccttatc cggcctacaa attctcaagc attcaatcag ttgcaatgcc tgctgtaggc
     1141 ctgataagcg aagcgcatca ggcaataata cgtttgtcac ccagcctctg gatcacatca
     1201 gttcgggcac ttataaacct gaccgataat ctggctatcc gtcgggacaa aactggacaa
     1261 cattccctgc gacgggctgc tgatgccata aatcacgtta ccgcccattg cagccgcctg
     1321 gttgcgcaga tcgtttgctg cgccgcgcat agaaccgccc tcttctccgt gttgcccgga
     1381 aagccagttg ctttgcttac ctgtcgcagt accaatcagc tggcactctg cgcctggctg
     1441 ctcgtccaca atgcgtacac tctgaccggc agcactcaat tcgttgctgg agctacaacc
     1501 cgccatcagt agcgcggcac cgacaatccc tgctaagtat tttacgtgca tgttattccc
     1561 cataatcaat gagctggacg ccactcgcgt catccgtgtc tgaatcttat actagaaaga
     1621 tgaccaaaag aaaaaccccc agacatttct gcccgggggt ttgtttattt ctgcgaagtg
     1681 cagggcaatt acatcatgcc gcccatgcca cccatgccgc ccataccgcc agcagcgcct
     1741 aagtcagctg catcgttttt cggcaggtcg gtaaccatgc attcggtggt gatcatcagg
     1801 ccagccacag aagctgcgta ctgcagagca gaacgggtta ctttggttgg gtccaggata
     1861 cccatgtcga tcatgttgcc gtattcttcg gttgctgcgt tgtaaccgta gttgccgtcg
     1921 ccgcctttaa cggtgttagc aacaacagac ggttcttcgc cgcagttcag gacgatctga
     1981 cgcagcggag cttccattgc acgcagtgca actttgatac ccacgttctg gtcttcgttc
     2041 tgaccacgca ggtcagccag tttagacgct acgcggatca gcgcaacacc accaccagca
     2101 accacgcctt cttctaccgc agcacgggtc gcgtgcaggg catcttcaac gcgtgctttt
     2161 ttctctttca tttcaacttc ggtagcagca cctactttga taactgcaac gccgcctgcc
     2221 agtttcgcta cgcgctcctg cagtttttca cggtcgtagt cagaagttgc ttcttcaatc
     2281 tgctgacgga tctgagcaac acggccctgg attgcagctt cttcacccac gccatcgatg
     2341 atagtggtgg tgtctttgtt gatcacaaca cgtttagcct gacccaggtc ttccagggtt
     2401 gctttttcca gctccatacc gatctcttca gagattacgg taccgccagt cagggttgcg
     2461 atatcctgca gcatagcttt acgacgatcg ccgaagcccg gagctttaac cgcagcaact
     2521 ttcacgatgc cacgcatggt gttaacaacc agagttgcta gcgcttcgcc ttcaacatct
     2581 tcagcgatga tcagcagcgg tttgcctgct ttggcaacag cttccagaac cggcagcatt
     2641 tcgcggatgt tggagatttt cttgtcagcc agcaggatga acgggctttc cagttctact
     2701 gcgccagttt ccggcttgtt gatgaagtaa ggagacaggt agccacggtc gaactgcata
     2761 ccttcaacca cgtccagttc gtcctgcaga ccggtaccgt cttcaacggt gataacgcct
     2821 tctttaccga ctttgtccat cgcttcagcg atcagtttac ctacggtttc gtcggagtta
     2881 gcggagatgg taccaacctg agcaatcgct ttagaatcag agcacggtac ggacagcgct
     2941 ttcagttctt caactgcagc ggtaaccgct ttgtcgatac cacgtttcag gtccatcggg
     3001 ttcatgcccg cagcaacagc tttcagacct tcagtgatga tagcctgagc cagtacggtt
     3061 gcagtggtgg taccgtcgcc tgcagcgtcg ttcgctttgg aggcaacttc tttcaccatc
     3121 tgcgcaccca tattttcgaa cttgtcttcc agttcgattt cacgagcaac ggaaacacca
     3181 tctttggtga tggtcggtgc accgaaagat ttatccagaa ctacgttacg gccttttgga
     3241 ccgagggtaa ctttcactgc atctgccagt acgtttacgc cgcgcagcat tttcacacga
     3301 gcgtcgttac cgaattttac gtctttagct gccattatct ttattcctta aattcgtatg
     3361 ttcagtgtcg tgcgcggatt acgcttcaac aattgccaga atgtcgcttt cggacatgat
     3421 caacacttct tcattgtcga tcttctcgga tttcacaccg tagccatcgt tgaaaataac
     3481 gatgtcgcca actttcacgt ccagcggctt cacttcgcca ttttcaagga tacggccatt
     3541 gccgacagcc agcacttcgc cgcgggtgga tttagccgct gcagagccgg tcagaacgat
     3601 gccgccagca gatttagttt caacttcttt acgcttgacg atcacgcgat catgcaatgg
     3661 acgaatattc attgataact ctcctttgag aaagtccgta tctgttatgg gtgacgccgg
     3721 agcttacgtg gtttcccggc tggtgaccag agaaatgggg atgaggcttc gccccttcaa
     3781 gggggaaaaa gaaaaaaaat tctgatcagc acaaaatcgg gtgaaaaccc tgattcacct
     3841 cacatttcat cgcaatttct tcatcgccgt cataagcgaa tctgattgtg ctaccatcga
     3901 aaatctacgc agttgcccaa aatttgggcg caatcggata tcaacccatg agtggactca
     3961 aacaagaact ggggctggcc cagggcattg gcctgctatc gacgtcatta ttaggcactg
     4021 gcgtgtttgc cgttcctgcg ttagctgcac tggtagcagg caataacagc ctgtgggcgt
     4081 ggcccgtttt gattatctta gtgttcccga ttgcgattgt gtttgcgatt ctgggtcgcc
     4141 actatcccag cgcaggcggc gtcgcgcact tcgtcggtat ggcgtttggt tcgcggcttg
     4201 agcgagtcac cggctggctg tttttatcgg tcattcccgt gggtttgcct gccgcactac
     4261 aaattgccgc cggattcggc caagcaatgt ttggctggca tagctggcaa ctgttgttgg
     4321 cagaactcgg tacgctggcg ttggtgtggt atatcggtac tctcggtgcc agttccagtg
     4381 ctaatctaca aaccgttatt gccggactta tcgtcgcact gattgtcgct atctggtggg
     4441 cgggcgatat caaacctgcg agtatcccct tccccgcgcc aggaaatatc gaacttaccg
     4501 ggttatttgc tgcgttatca gtgatgttct ggtgttttgt cggtctggaa gcatttgccc
     4561 atcttgcctc ggaatttaaa aatccagagc gtgattttcc tcgtgctttg atgattggtc
     4621 tgctgctggc aggattagtc tactggggct gtacggtagt cgtcttacac ttcgacgcct
     4681 atggtgaaca aatggcggcg gcagcatcgc ttccaaaaat tgtagtacag ttgttcggtg
     4741 taggagcgtt atggattgcc tgcgtaattg gctatctggc ctgctttgcc agtcttaaca
     4801 tttatataca gagcttcgcc cgcctggtct ggtcgcaggc gcaacataat cctgaccact
     4861 acctggcacg cctctcttct cgccatatcc cgaataatgc cctcaatgcg gtgcttggct
     4921 gctgcgtggt gagcacgttg gtaattcatg ccttagagat caatctggac gctcttatta
     4981 tttatgccaa tggcatcttt attatgattt atctgttatg catgctggca ggctgtaaat
     5041 tattgcaagg tcgttatcga ctactggcgg tggttggcgg gctgttatgc gttctgttac
     5101 tggcaatggt cggctggaaa agtctctatg cgctgatcat gctggtgggg ttatggctgt
     5161 ttctgccaaa acgaaaaacg ccggaaaatg gcataaccac ataatccggc gtttcgacat
     5221 taatcctggc gatcgtcttt atgatcaagg cggtcgcggt catcatcctt tcgctgatac
     5281 tcaccatcaa aagtattacc gccaccggtc ccggcgctaa aaccgccgcc tggcatgcga
     5341 gaaaagcgca aatgtggcat caacttcact gtcagatgct tttgcaccgg cggcaataaa
     5401 agcagaagac cgaggaagtc ggtgaaaaag cccggtaata aaagcagcaa accggcaata
     5461 atcagcgaaa cacttttaat catctccgcc gcagggtttt cacccgccgc cattttttgc
     5521 tgcatcagca cgaaattctt aaagccctgg ttacgtacca gtgacatacc gataactgac
     5581 gtgaatataa ccagcacgag ggtcagcaat acccccaata catgggcaac ctgaataaag
     5641 attgaaatct caatatagac ataaaggaaa atggcaataa aaggtaacca gcgcaaaggt
     5701 ttctcctgta atagcagccg gttaaacccg gctacctgaa tgggttgcga atcgccttca
     5761 gcttatattg tggtcattag caaaatttca agatgtttgc gcaactattt ttggtagtaa
     5821 tcccaaagcg gtgatctatt tcacaaatta ataattaagg ggtaaaaacc gacacttaaa
     5881 gtgatccaga ttacggtaga aatcctcaag cagcatatga tctcgggtat tcggtcgatg
     5941 caggggataa tcgtcggtcg aaaaacattc gaaaccacat atattctgtg tgtttaaagc
     6001 aaatcattgg cagcttgaaa aagaaggttc acatgtcaaa caacattcgt atcgaagaag
     6061 atctgttggg taccagggaa gttccagctg atgcctacta tggtgttcac actctgagag
     6121 cgattgaaaa cttctatatc agcaacaaca aaatcagtga tattcctgaa tttgttcgcg
     6181 gtatggtaat ggttaaaaaa gccgcagcta tggcaaacaa agagctgcaa accattccta
     6241 aaagtgtagc gaatgccatc attgccgcat gtgatgaagt cctgaacaac ggaaaatgca
     6301 tggatcagtt cccggtagac gtctaccagg gcggcgcagg tacttccgta aacatgaaca
     6361 ccaacgaagt gctggccaat atcggtctgg aactgatggg tcaccagaaa ggtgaatatc
     6421 agtacctgaa cccgaacgac catgttaaca aatgtcagtc cactaacgac gcctacccga
     6481 ccggtttccg tatcgcagtt tactcttccc tgattaagct ggtagatgcg attaaccaac
     6541 tgcgtgaagg ctttgaacgt aaagctgtcg aattccagga catcctgaaa atgggtcgta
     6601 cccagctgca ggacgcagta ccgatgaccc tcggtcagga attccgcgct ttcagcatcc
     6661 tgctgaaaga agaagtgaaa aacatccaac gtaccgctga actgctgctg gaagttaacc
     6721 ttggcgcaac agcaatcggt actggtctga acacgccgaa agagtactct ccgctggcag
     6781 tgaaaaaact ggctgaagtc actggcttcc catgcgtacc ggctgaagac ctgatcgaag
     6841 cgacctctga ctgcggcgct tatgttatgg ttcacggcgc gctgaaacgc ctggctgtga
     6901 agatgtccaa aatctgtaac gacctgcgct tgctctcttc tggtccacgt gccggcctga
     6961 acgagatcaa cctgccggaa ctgcaggcgg gctcttccat catgccagct aaagtaaacc
     7021 cggttgttcc ggaagtggtt aaccaggtat gcttcaaagt catcggtaac gacaccactg
     7081 ttaccatggc agcagaagca ggtcagctgc agttgaacgt tatggaaccg gtcattggcc
     7141 aggccatgtt cgaatccgtt cacattctga ccaacgcttg ctacaacctg ctggaaaaat
     7201 gcattaacgg catcactgct aacaaagaag tgtgcgaagg ttacgtttac aactctatcg
     7261 gtatcgttac ttacctgaac ccgttcatcg gtcaccacaa cggtgacatc gtgggtaaaa
     7321 tctgtgccga aaccggtaag agtgtacgtg aagtcgttct ggaacgcggt ctgttgactg
     7381 aagcggaact tgacgatatt ttctccgtac agaatctgat gcacccggct tacaaagcaa
     7441 aacgctatac tgatgaaagc gaacagtaat cgtacagggt agtacaaata aaaaaggcac
     7501 gtcagatgac gtgccttttt tcttgtgagc agtaacttaa aaataacaat ctaatatcaa
     7561 cttgttaaaa aacaaggaag gctaatatgc tagttgtaga gctcatcata gttttgctgg
     7621 cgatcttctt gggcgccaga ttggggggaa taggtattgg ttttgcaggc ggattggggg
     7681 tgctggttct tgccgctatt ggcgttaaac ccggtaacat cccgttcgat gtcatttcca
     7741 ttatcatggc ggttatcgcc gctatttctg ccatgcaggt tgctggcggt ctggactatc
     7801 tggttcatca gacagaaaag ctgctgcgcc gtaacccgaa atacatcacg atcctcgcac
     7861 cgatcgtgac ctatttcctg actatctttg ctggtactgg caacatctct ctggcgacac
     7921 tgccagttat cgctgaagtt gcgaaggaac aaggcgttaa accttgccgt ccgctctcta
     7981 ctgcagtggt atccgcgcag attgcgatca ccgcatcgcc aatctcagcg gcagtggttt
     8041 acatgtcttc cgtgatggaa ggtcatggca tcagctacct ccatctgctc tccgtggtca
     8101 tcccgtccac cctgctggcg gttctggtga tgtccttcct ggtcactatg ctgttcaact
     8161 ccaaactctc tgacgatccg atttatcgca agcgtctgga agagggcttg gttgaactgc
     8221 gcggtgaaaa gcagattgaa atcaaatccg gtgcaaaaac gtccgtctgg ctgttcctgc
     8281 tgggcgtagt tggcgtggtt atctatgcaa tcatcaacag cccaagcatg ggtctggttg
     8341 aaaaaccgct gatgaacacc accaacgcaa tcctgatcat catgctcagc gttgcaactc
     8401 tgaccaccgt tatctgtaaa gtcgataccg acaacatcct caactccagc accttcaaag
     8461 caggtatgag cgcctgtatt tgtatcctgg gtgttgcgtg gctgggcgat actttcgttt
     8521 ccaacaacat cgactggatc aaagataccg ctggtgaagt gattcagggt catccgtggc
     8581 tgctggccgt catcttcttc tttgcttctg ctctgctgta ctctcaggct gcaaccgcaa
     8641 aagcactgat gccgatggct ctggcactga acgtttcacc gctgaccgct gttgcttctt
     8701 tcgctgcggt gtctggtctg ttcattctgc cgacctaccc gacgctggtt gctgcggtac
     8761 agatggatga cacgggtact acccgtatcg gtaaattcgt cttcaaccat ccgttcttca
     8821 tcccgggtac tctgggtgtt gccctggccg tttgcttcgg cttcgtgctg ggtagcttca
     8881 tgctgtaatg atcaatcgcg gggcgttcac gccccgcttt ctttcccgcc gactaacatc
     8941 cttcccccgt ccgttgtata gtgacctctc tcttgcggtt ccatctgttc ttgcgaggtg
     9001 tttatgcttg atgaaaaaag ttcgaatacc acgtctgtcg tggtgctatg taccgcaccg
     9061 gatgaagcga cagcccagga tttagccgcc aaagtgctgg cggaaaaact ggcggcctgc
     9121 gcgaccttga tccccggcgc tacctctctc tattactggg aaggtaagct ggagcaagaa
     9181 tacgaagtgc agatgatttt aaaaactacc gtatctcacc agcaggcact gctggaatgc
     9241 ctgaagtctc atcatccata tcaaaccccg gaacttctgg ttttacctgt tacacacgga
     9301 gacacagatt acctctcatg gctcaacgca tctttacgct gattctgcta ctttgcagca
     9361 cttccgtttt tgccggatta ttcgacgcgc cgggacgttc acaatttgtc cccgcggatc
     9421 aagcctttgc ttttgatttt cagcaaaacc aacatgacct taatctgacc tggcagatca
     9481 aagacggtta ctacctctac cgtaaacaga tccgcattac gccggaacac gcgaaaattg
     9541 ccgacgagca gctgccgcaa ggcgtctggc atgaagatga gttttacggc aaaagcgaga
     9601 tttaccgcga tcggctgacg cttcccgtaa ccatcaacca ggcgagtgcg ggagcgacgt
     9661 taactgtcac ctaccagggc tgtgctgatg ccggtttctg ttatccgcca gaaaccaaaa
     9721 ccgttccgtt aagcgaagtg gtcgccaaca acgcagcgtc acagcctgtg tctgtttcgc
     9781 agcaagagca gcacaccgcg caattgccct tttccgcgct ctgggcgttg ttgatcggta
     9841 ttggtatcgc ctttacgcca tgcgtgctgc caatgtaccc actgatttct ggcatcgtgc
     9901 tgggtggtaa acagcggctc tccactgcca gagcattgtt gctgaccttt atttatgtgc
     9961 aggggatggc actgacttac acggcgctgg gtctggtagt tgccgccgca gggttacagt
    10021 tccaggcggc gctacagcac ccatacgtgc tcattggcct cgccatcgtc tttaccttgc
    10081 tggcgatgtc aatgtttggc ttgtttactc tacaactccc ctcttcgctg caaacacgtc
    10141 tcacgttgat gagcaatcgc caacagggcg gctcacctag cggtgtgttt gttatggggg
    10201 cgattgccgg actgatctgt tcaccatgca ccaccgcacc gcttagcgcg attctgctgt
    10261 atatcgccca aagcgggaac atgtggccgg gcggcggcac gctttatctc tatgcgttgg
    10321 gcatgggcct gccgctgatg ctaattaccg tctttggtaa ccgcttgctg ccgaaaagcg
    10381 gcccgtggat ggaacaagtc aaaaccgcgt ttggttttgt gatcctcgca ctgccggtct
    10441 tcctgctgga gcgagtgatt ggtgatgtat ggggattacg cttgtggtcg gcgctgggtg
    10501 tcgcattctt tggctgggcc tttatcacca gcctacaggc taaacgcggc tggatgcgcg
    10561 tggtgcaaat aatcctgctg gcagcggcat tggttagcgt gcgcccactt caggattggg
    10621 catttggtgc gacgcatacc gcgcaaactc aaacgcatct caactttaca caaatcaaaa
    10681 cggtagatga gttaaatcag gcgctcgttg aagccaaagg caaaccggtg atgttagatc
    10741 tttatgccga ctggtgcgtc gcctgtaaag agtttgagaa atacaccttc agcgacccgc
    10801 aggtgcaaaa agcgttagca gacacggtct tacttcaggc caacgtcacg gccaacgacg
    10861 cacaagatgt ggcgctgtta aagcatctta atgtccttgg cctaccgaca attctctttt
    10921 ttgacggaca aggccaggag catccacaag cacgcgtcac gggctttatg gatgctgaaa
    10981 ccttcagcgc acatttgcgc gatcgccaac cgtgaacgac acttgcagtg ggaaagacgg
    11041 aggaaaatag cgtgcaacgt gaagatgtac tgggagaagc cctgaaatta ttagaattac
    11101 aagggattgc caacaccacg ctggagatgg ttgctgaacg tgtggattat ccactggacg
    11161 agctacgccg cttctggcca gacaaagagg caatcctcta cgatgcgctg cgttatctta
    11221 gccaacagat agatgtctgg cgtcgtcagc tgatgctgga cgaaacgcaa accgccgaac
    11281 aaaagctgct ggcacgttat caggcgttat cggagtgcgt taaaaacaac cgctatccgg
    11341 gctgtctatt tatcgctgcc tgtacgtttt atcccgatcc tggccaccct attcatcaac
    11401 tggccgatca gcaaaaaagc gctgcctacg atttcaccca cgaactgtta accacgctgg
    11461 aagttgacga tccggcgatg gtagcaaagc agatggaact ggtgctggaa ggctgtttaa
    11521 gccgaatgct ggtgaatcgt agccaggcgg atgtcgacac tgcacatcgg ctggcggaag
    11581 atatcctgcg cttcgcccgc tgccgccagg gtggtgcatt gacctgacag aaacacagaa
    11641 aagaagcgat ttgccgcaat cttaagcagt tgaatcgctt ttactgaaat taggttgacg
    11701 agatgtgcag atta
//
LOCUS       DAJTRD010000111        11684 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTRD010000111 DAJTRD010000000
VERSION     DAJTRD010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11684
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.111"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..348
                     /locus_tag="OO315_003401"
     CDS             <1..348
                     /locus_tag="OO315_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5892188.1"
                     /translation="SGNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRL
                     QQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQ
                     NDLLLRATGGVCV"
     gene            complement(465..740)
                     /gene="yjfN"
                     /locus_tag="OO315_003402"
     CDS             complement(465..740)
                     /gene="yjfN"
                     /locus_tag="OO315_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5892189.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(889..1218)
                     /gene="bsmA"
                     /locus_tag="OO315_003403"
     CDS             complement(889..1218)
                     /gene="bsmA"
                     /locus_tag="OO315_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5892190.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1400..2149
                     /gene="yjfP"
                     /locus_tag="OO315_003404"
     CDS             1400..2149
                     /gene="yjfP"
                     /locus_tag="OO315_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5892191.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2146..2901)
                     /gene="ulaR"
                     /locus_tag="OO315_003405"
     CDS             complement(2146..2901)
                     /gene="ulaR"
                     /locus_tag="OO315_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5892192.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3009..4073)
                     /gene="ulaG"
                     /locus_tag="OO315_003406"
     CDS             complement(3009..4073)
                     /gene="ulaG"
                     /locus_tag="OO315_003406"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5892193.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4428..5825
                     /gene="ulaA"
                     /locus_tag="OO315_003407"
     CDS             4428..5825
                     /gene="ulaA"
                     /locus_tag="OO315_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5892194.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5841..6146
                     /gene="ulaB"
                     /locus_tag="OO315_003408"
     CDS             5841..6146
                     /gene="ulaB"
                     /locus_tag="OO315_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5892195.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6156..6620
                     /gene="ulaC"
                     /locus_tag="OO315_003409"
     CDS             6156..6620
                     /gene="ulaC"
                     /locus_tag="OO315_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5892196.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6634..7284
                     /gene="ulaD"
                     /locus_tag="OO315_003410"
     CDS             6634..7284
                     /gene="ulaD"
                     /locus_tag="OO315_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5892197.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7294..8148
                     /gene="ulaE"
                     /locus_tag="OO315_003411"
     CDS             7294..8148
                     /gene="ulaE"
                     /locus_tag="OO315_003411"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5892198.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8148..8834
                     /gene="ulaF"
                     /locus_tag="OO315_003412"
     CDS             8148..8834
                     /gene="ulaF"
                     /locus_tag="OO315_003412"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5892199.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8963..9238)
                     /locus_tag="OO315_003413"
     CDS             complement(8963..9238)
                     /locus_tag="OO315_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5892200.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9565..9960
                     /gene="rpsF"
                     /locus_tag="OO315_003414"
     CDS             9565..9960
                     /gene="rpsF"
                     /locus_tag="OO315_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5892201.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9967..10281
                     /gene="priB"
                     /locus_tag="OO315_003415"
     CDS             9967..10281
                     /gene="priB"
                     /locus_tag="OO315_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5892202.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10286..10513
                     /gene="rpsR"
                     /locus_tag="OO315_003416"
     CDS             10286..10513
                     /gene="rpsR"
                     /locus_tag="OO315_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5892203.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10555..11004
                     /gene="rplI"
                     /locus_tag="OO315_003417"
     CDS             10555..11004
                     /gene="rplI"
                     /locus_tag="OO315_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5892204.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11075..11638)
                     /locus_tag="OO315_003418"
                     /pseudo
     CDS             complement(11075..11638)
                     /locus_tag="OO315_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2767 a         2873 c         3087 g         2957 t
ORIGIN      
        1 tccggcaata ttatgtgcct ggatgtgttg cgcgttctca ataagcaagc gggcgtatac
       61 gacttattgt cggaagcatt tgtggaagtg aaagggcagg atcgctattt tgatcgcgcg
      121 gttcgtcgtt tacagcagca gctgcgtaag ccagctgaag aactggggcg agagattact
      181 catcagctat tcctgctggg ctgcggtgcg caaatgttga aatatgcttc tccgccaatg
      241 gcgcaggcgt ggtgtcaggt gatgttagat acgcgcggcg gcgtacggtt gtcagagcag
      301 atccagaatg atttattgct gcgggcgacg gggggagtgt gtgtgtaagc gtatacgact
      361 gatgcgacgc tggtttcgat taactaaatg aaatatgtga aaattgtagg ccggacaagg
      421 cgctcgcgcc gcatccggca ttgttcattt gctgtagatg acactcatgc atacagtatc
      481 gcctgtacgc gccagttatc aggctgctgg tcttcgtaca tggtaataat gcggtaccat
      541 gacgcacctt gttcatcggc ctttaacgcc acgatacgtt ccacgtcctg cggatcccct
      601 gaaatattgc taactgagat ctgacctatt tcattcaggc ccgtcacgca gtcagcactg
      661 gcgaattctg cagactgtgc cgtggcgctc gcaggataag tcactaattg cagcgagggt
      721 gaggcaagta attgtttcat cgtcagctcc atttttcgtt tccccacatg ttgcagggca
      781 ttccatcgcg gtaataaacg cccaagcttc cagccggtaa tttattgtgc acaggtaaac
      841 gcatagggac gcaaagcaat gtaaagctga ctatgtaagt tgtctgattt atttacggta
      901 caaaatggct tgtgaatacc actgtcctgt cacgatggtt tcgtccacca tgacgacaac
      961 gtaataatca gcttttgcag cgacagcttt tgctctgatt tctgctaatg cgtcatccgg
     1021 agaaccccga accatcgtgc ttacgctacc tattcgctgt aacccttgcg tctggtcgcg
     1081 gcgaatctct tgcggatggt ccgttactgg cggtgctggc tgtggcgtac cttgcagtgc
     1141 gctacaggca cttaacatca acaccaataa taaactggca aaccggtaaa taacgctatt
     1201 acgtttcctg ctaaccatag tgtagtgcct tattaattta actttggggg aatgttcccg
     1261 aattgttacc tggtacgcga ttttcgaatg aaaacgtgac gaaagcgtaa cccacatctc
     1321 aacattatga actaataccg ccagcagaaa cttgtgctaa ggatttcttc atgtcatacc
     1381 agacaaaaag gagagacaga tgattgaaat agaatcacgc gagctggcag atattcccgt
     1441 tcttcatgct tatcctgtcg ggcaaaaaga taccccgtta ccgtgcgtaa ttttttatca
     1501 cggctttact tcatccagcc tggtgtatag ctattttgcc gttgcgctgg cgcaggctgg
     1561 tttgcgggtg atcatgccgg atgcgcccga tcacggtagc cgttttagtg gcgacgaagc
     1621 gcggcggtta aatcaattct ggcaaatctt gctacaaagt atgcaggaat tcactacttt
     1681 acgtgcggca atagccgaag aaaactggct gcttgatgac cgtctggcag tcggtggcgc
     1741 gtcgatgggc gcgatgacgg cactgggaat taccgctcgc caccccacgg tgaaatgtac
     1801 cgccagcatg atgggatcgg gctattttac atcgctcgcc cgttcactgt ttccaccgct
     1861 gatacctgaa acggcagcac agcagaatga attcaataaa attgtcgcgc cgctggcaga
     1921 gtgggaagcg acaaaccacc tggaacaact tggggacaga cctctactgc tgtggcatgg
     1981 cctcgacgat gacgttgtgc ctgccgacga atcactacgt ttgcagcagg ccttaagcga
     2041 gacgggacgg gataaactgc taacctgttc atggcagcca ggcgtgcgtc accgcattac
     2101 gcctgaggcg ttagatgctg ccgtgacatt tttccgccag catctttaaa cacgcagaat
     2161 gctgacccct tgcgcttcca gttgttgcag gatttccggg ttagcatttt tgccggtgat
     2221 cagcatatcg atttgatcgg cacggctaaa aagcattccc gcgcgttcgc caatcttact
     2281 gctatcaacc agtaccacca gtttccctac cacgctcagc atcttctgct ctgccattgc
     2341 tgtcagcata tcggttttat acaacccttc tgcggtcagc ccttttccgc tggtaaacat
     2401 ccagtgcccg gcatagagac tgttttcgct gccctgcggg cttaaagtga tggactgact
     2461 tttgttgtac tgtccaccca taatgatcac gctgtcatgt tcttgatcga tcaggtaatt
     2521 tgccagcggt agataattag tgatgatttg cactggcttg ccacacattt cccgcccaag
     2581 cagaaacgcg gtggagccgc agttgatgac tacgctttcg cccggattaa ccagctgcga
     2641 ggccgcttta gcaatacgta ctttttcatc gtgattctgc gcctgatgca gattcatcgg
     2701 cgtccagcgc gggcgctgtt gggtaatagc ttctgcgcca ttgcgcacct ttttcagttt
     2761 gccgctttcg ccaagtttat tgatatcgcg tcgcgcagtg gcaggcgaaa ttcccagacg
     2821 ctcaacgact ttctcaacgg tcacaaagcc caattgtgcg agcatttcca ggaggatttg
     2881 atgtctttgt gcttcagtca tgagctattc cgataaaatt tgattttttt agatgatatt
     2941 tgaaatagcc aggaaatact acgccggata gcgcgaactt ctccacgcta tccggtataa
     3001 acgacagatt acaggaatga cttgaacggc agatccggtt caatagtgaa gcaatcatcg
     3061 aaaccgcgcg gatagtggta ctcgaagttg tctttatcca gcggccaggt aaatttgccg
     3121 ccaacctgcc agataaacgg cttgaagcca tacttcagac gatctttttt gatctcccac
     3181 agcacgcgga tctcttgcgg atcggcctgg aagtttgacc agatgtcgtg gtggaacggg
     3241 ataaccactt tcgcattcag ggcttcaccc atacgcagca tatcggcgct ggtcattttg
     3301 tcggtaatac cgcgcgggtt ctcaccatac gatcctaacg ccacatcgat ctgatgttcg
     3361 ttaccgtgct tcgcataata gttagagtag tgggaatcgc cactgtgata tagggaaccg
     3421 ccaggggttt tgaacaggta gtttactgcg cgatcgtcca tgccatctgg cagtacgcca
     3481 gccgcttttt gatcggcagg cagggtgatc agtgcagtac ggtcgaaagc gtcaagcgca
     3541 tgaatttcaa tgtctttcac ttttacaaca tcgcccggtt tgaccacgat gcaacgctct
     3601 ttcggtacgc cccagccaat ccacaaatca acgcaggttt tcggtccaat gaatggtacg
     3661 tcatcagcac agttctgcat cacggcagca gcgacgttaa cgtcgatatg atcgttgtga
     3721 tcgtgagtcg ccagtaccgc gtcgatctgg cgaatcgcaa acggatcaag aacaaacggg
     3781 gtggtgcgta ggtttggctg cagttttttc acgccagcca tgcgctgcat ctggtgaccc
     3841 tgtttcatta acgggttacc gtgactttgt ttgccagtgc cgcaccagaa atcaacgcaa
     3901 acgttggtgc caccttctga tttcaaccag atcccggtgc agcctagcca ccacatcgca
     3961 aatgtgccag gggcgacctg ctcttgttca atttcttcat tcagccagct accccactcc
     4021 gggaaagtgc tcaggatcca ggattctcgg gtgatacttt tcactttact catcgccatt
     4081 taccttcatg atagttcaat tcgaatcaac atgtgattgg ttttgattaa tcctgacact
     4141 attccttcag gaaggtaatg accatttttt gactttttgc cagggaagtt gttgttgatt
     4201 ttgagtgggg aaagatttaa tggaatgtgt agttaaatta actaaatgaa tttaaataaa
     4261 taattgtttc gttgtgtgaa tcccactcta tccatgtgga attatttgcg ggtcgcgtca
     4321 catttaatca taaataatct tgttgtgatt acttttgaaa attagagtga gtgcacaaca
     4381 ttccgggtgt gcggaatacc cggttacctc ttcttcagga gatcgttatg gagatcctct
     4441 acaacatctt taccgtgttt tttaaccagg tcatgaccaa tgccccgttg ttgctgggta
     4501 ttgtgacctg tctgggctac atcctactgc gcaaaagtgt cagcgttatt attaaaggca
     4561 cgattaaaac cataattggt ttcatgttgt tgcaggcagg gtccggcatc ctcaccagca
     4621 ccttcaaacc ggtggtggcg aaaatgtccg aagtctacgg cattaacggc gcaatttccg
     4681 atacttacgc gtcgatgatg gcaaccatcg accgcatggg cgatgcctat agctgggtag
     4741 gttacgcggt gttgttagcg ctggcgctga acatctgtta cgtgctgctg cgtcgcatta
     4801 ccggtattcg taccatcatg ctgactggtc atatcatgtt ccagcaggcg gggctgattg
     4861 ccgttacgtt gtttattttc ggctactcca tgtggacaac cattatctgc acggcgattc
     4921 tggtttcgct ctactggggc attacctcca acatgatgta caagccgact caggaagtga
     4981 cggacggctg cggtttctcc atcggtcacc agcaacagtt tgcatcatta attgcctata
     5041 aagttgcgcc gttcctcggc aaaaaagagg aaagcgttga agacctcaaa ctgccaggct
     5101 ggctgaatat tttccacgac aacatcgtct ccacggcgat tgtcatgacc atcttctttg
     5161 gtgccattct gctctccttc ggtatcgaca ccgtgcaggc gatggcaggc aaagtgcact
     5221 ggacggtcta tatcctgcaa accggttttt ccttcgcggt ggcgatcttc atcatcactc
     5281 agggtgtgcg catgtttgtg gcggaactct ctgaagcgtt taacggtatc tcccagcgcc
     5341 tgatccctgg tgcggttctg gcgattgact gtgcggctat ttatagcttc gctccgaacg
     5401 ccgtggtctg gggctttatg tggggcacca tcggtcagct gattgcggtt ggcatcctgg
     5461 tcgcctgcgg ttcctcgatc ctgattatcc caggctttat cccgatgttc ttctccaacg
     5521 ccaccatcgg cgtgttcgct aaccacttcg gcggctggcg tgcggcgctg aagatttgtc
     5581 tggtgatggg gatgatcgaa atctttggtt gcgtctgggt ggtgaaactt accggtatga
     5641 gtgcctggat ggggatggcg gactggtcga ttctggcacc gccgatgatg cagggcttct
     5701 tctccatcgg tatcgccttt atggccgtca tcattgtaat tgcactggct tatatgttct
     5761 tcgctggccg cgcactgcgc gcagaagaag atgcagaaaa acaattggca gaacagtctg
     5821 cttaataagg agttttgatt atgaccgtac gtattctggc tgtgtgtggc aacggacaag
     5881 gcagttccat gatcatgaag atgaaagtgg accagttttt aacccaatca aacattgacc
     5941 acacggtaaa tagctgcgcg gttggcgaat acaaaagcga gttgagcggc gcggatatca
     6001 tcatcgcttc tacgcacatt gcgggcgaaa tcaccgtgac cggcaacaaa tacgtggttg
     6061 gcgtgcgcaa catgctctct cctgccgact ttggcccgaa actgctggaa gtgatcaaag
     6121 cgcatttccc gcaggatgtg aagtaaggac gcgccatgaa attacatgat tcgctggcgg
     6181 agaataaatc catccgcctg caggctgaag ctgagacatg gcaggacgca gtaaaaatcg
     6241 gcgttgacct gctggtggcg gcggatgttg tcgagccgcg ttactaccag gcgattctgg
     6301 atgcggttga acagcacggt ccgtactttg tgctcgcacc aggcctggca atgccgcacg
     6361 gtcgcccgga agagggcgtt aagaaaaccg gttttgcgct ggtgacgctg aaaaagccgc
     6421 tggagttcaa ccacgaagat aacgacccgg tggatatcct catcaccatg gcggcggtcg
     6481 atgccaacac gcaccaggaa gtgggcatca tgcagatcgt caacctgttt gaagatgaag
     6541 aaaatttcga ccgtttacgt gcctgccgta ccgagcagga agtactggat ctcattgacc
     6601 gcaccaacgc ggcagcttaa gaaggaattg aagatgtcat taccgatgtt gcaagtcgcg
     6661 ctggacaacc agactatgga tagcgcctac gaaaccactc gcctgattgc cgaagaagtc
     6721 gacattatcg aagtgggtac cattctgtgc gtgggcgaag gcgtgcgtgc ggttcgcgat
     6781 ctgaaagcgc tctacccgca caaaatcgtg ctggcagacg ccaaaattgc cgatgcgggc
     6841 aaaattcttt ctcgtatgtg cttcgaagcc aacgctgact gggtgaccgt gatttgttgt
     6901 gcggatatca acaccgccaa aggtgcgctg gacgtagcaa aagagtttaa cggcgacgtg
     6961 cagatcgaac tgaccggtta ctggacctgg gaacaggcgc aacagtggcg cgacgcaggt
     7021 attcagcagg tggtttatca ccgcagccgt gacgcgcagg ccgctggcgt ggcgtggggt
     7081 gaagccgata tcactgcgat caaacgtctt tccgatatgg gcttcaaagt caccgtcacc
     7141 ggtggcctgg cgctggaaga tctgccgctg ttcaaaggca ttccaattca tgtgtttatc
     7201 gccggtcgca gcatccgtga tgccgcttct ccggttgaag ccgcgcgtca gttcaaacgt
     7261 tccatcgccg aactgtgggg ctaaggagcg gatatgttgt ccaaacaaat cccgcttggc
     7321 atctatgaaa aagcgctccc cgccggggag tgctggctgg aacgcctgcg actggcaaaa
     7381 acgttgggct tcgattttgt cgagatgtcg gtagatgaaa ctaacgagcg cctgtcgcgc
     7441 ctcgactgga gccgcgaaca gcgtctggcg ctggtgaatg ctattgttga aaccggcgtg
     7501 cgtgtgccgt ccatgtgcct ttctgcccat cgtcgtttcc cgttgggcag cgaagatgac
     7561 gcggtgcggg cgcaggggct ggagattatg cgtaaagcca tccagttcgc ccaggacgtt
     7621 ggcattcgcg tgatccagct ggcgggctat gacgtttact atcaggaagc caataacgaa
     7681 acgcgtcgtc gtttccgtga cggcctgaaa gagagcgttg agatggcgag ccgcgcgcag
     7741 gtgacgctgg cgatggagat catggattat ccgttgatga actccatcag caaggcgctg
     7801 ggttacgcgc actatctcaa caacccgtgg ttccagctct acccggatat cggcaacctg
     7861 tcggcgtggg acaacgatgt gcagatggag ttgcaggccg gaatcgggca tatcgtcgcg
     7921 gtgcatgtga aagacaccaa accgggcgtc ttcaaaaacg tgccatttgg cgaaggtgtg
     7981 gtggatttcg aacgttgctt cgaaacgctc aaacagagtg gctattgcgg gccgtacctg
     8041 attgagatgt ggagcgaaac ggcggaagac ccggcggcag aagtggtgaa agcgtgtgat
     8101 tgggtgaaag cgcgcatggc caaagcaggc atggtggagg cggcataatg caaaagctaa
     8161 aacagcaggt gtttgaagcc aacatggatc tgccgcgcta tgggctggtg acctttactt
     8221 ggggcaacgt cagcgctatc gaccgcgaac gcgggctggt ggtgatcaag cccagcggcg
     8281 ttgcctacga aaccatgaaa gcagacgata tggtggtggt cgatatgagc ggcaaggtgg
     8341 tggaagggga gtatcgccca tcttccgaca ctgcgacgca tctcgaactc taccgtcgtt
     8401 acccgtcgct tggcggcatt gtccataccc actccactca tgccaccgca tgggcgcagg
     8461 cggggctggc gatcccggca ttaggcacca cgcacgctga ctacttcttt ggtgatatcc
     8521 cgtgtacgcg tggattaagc gaagaagagg tgcagggtga gtatgaactg aacactggca
     8581 aagtgattat cgaaacgctg ggcaacgccg agccgctgca tacgccggga attgtggtgt
     8641 atcagcacgg accgttcgcc tgggggaaag atgcccacga cgcggtgcat aacgcggtag
     8701 tgatggaaga agtggcgaaa atggcgtgga ttgcgcgcag tattaatcca caactcaatc
     8761 atatcgacag ctacctgatg aataaacact tcatgcgtaa gcacggtcct aacgcttatt
     8821 acgggcagaa gtaaaacacg cgctgcggaa atttccttcc tcgggagata actggtctaa
     8881 ttccgcagcc gtttttcaaa aaaagccccc tgcgaagggg gcaaagcaaa ctatggcaat
     8941 gtttcgttgg ttatacctgg tgctagcgat aaatatccgc gctggcgtgc atattgccgt
     9001 tactccccgg ttcccgcatc agaattacgt ggtagtacgt tgcgccttgc gcatctgttt
     9061 cttcatttaa tgcctgacgt gcttcgcttt cagtggcgaa attatgattg atataaatca
     9121 cgcctaagct ttgcacatcg tccatatttc tggcctggtg gttattaatt tcaatggctg
     9181 cccatgtatt tgcacttagc aaaagcacag ccagaagggc taaaactcga ctgaacatag
     9241 atacctcctc gacggctgac tttgtgtgct ctcctctgtg atgatcttct gatttaattt
     9301 taatcaatga taaagaagtt gatggtgacc atttctgatg cagttgttca aaaaaacact
     9361 gtgacggaat gtgatgaact tcaaatcagc ggtttagagg ttatttgcga aaggggagat
     9421 ttatttctgc tccgcccttg agtttagcgg ggtatgccag tactataacg cgtcattttt
     9481 cagccgacct ttaacacgtt ccttgcctcc ccgggattcg gctgacccag acaggaggct
     9541 gaataatccg taaggagcaa ttcgatgcgt cattacgaaa tcgtttttat ggtccatcct
     9601 gaccagagcg aacaggttcc gggcatgatc gagcgctaca ctgctgccat cactggtgca
     9661 gaaggcaaga tccaccgtct ggaagactgg ggccgccgtc agctggctta cccgatcaac
     9721 aaactgcaca aagcacacta cgttctgatg aacgttgaag ctccgcagga agtgatcgat
     9781 gagctggaaa ctaccttccg cttcaacgat gccgttatcc gcagcatggt tatgcgtacc
     9841 aagcacgctg ttaccgaagc atctccgatg gttaaagcga aagacgagcg ccgtgagcgt
     9901 cgcgatgatt tcgcaaacga aaccgctgat gatgctgaag ctggggattc tgaagagtaa
     9961 tttctgatga ccaaccgtct ggtgttgtcc ggcaccgtgt gcaggactcc ccttcgaaag
    10021 gtcagtccat caggaattcc tcactgccag ttcgtgcttg agcatcgttc tgtgcaggag
    10081 gaagccggct ttcaccggca ggcatggtgt caaatgcccg ttattgttag cggacacgaa
    10141 aaccaggcca ttactcacag tataacggtc ggcagtcgca taaccgttca ggggttcatt
    10201 tcatgccaca aggcaaagaa cggactgagc aaaatggttt tgcatgccga gcagattgaa
    10261 ttgatagatt ctggagacta gccatatggc acgttatttc cgtcgtcgca agttctgccg
    10321 tttcaccgcg gaaggcgttc aagagatcga ctataaagat atcgctacgc tgaaaaacta
    10381 catcaccgaa agcggtaaga ttgtcccaag ccgtatcacc ggtacccgtg caaaatacca
    10441 gcgtcagctg gctcgcgcta tcaaacgcgc tcgctacctg tccctgctgc cgtacactga
    10501 tcgccatcag taatcggtca cggtccatta atacgacttt gagaggataa ggtaatgcaa
    10561 gttattctgc ttgataaagt agcaaacctg ggtagcctgg gtgatcaggt aaacgttaaa
    10621 gcgggctatg ctcgtaactt cctggtaccg cagggtaaag ctgttccagc taccaagaaa
    10681 aacattgaat tcttcgaagc acgtcgcgct gaactggaag ctaaactggc tgaagttctg
    10741 gcagctgcta atgctcgcgc tgagaaaatc aatgcactgg aaactgttac catcgcgtct
    10801 aaagctggcg acgaaggtaa actgttcggt tccatcggta ctcgcgacat cgctgacgct
    10861 gtaactgcag ctggcgttga agtggctaag agcgaagttc gtctgccgaa cggcgttctg
    10921 cgtaccactg gcgaacacga agtgagcttc caggttcaca gcgaagtatt cgcgaaagtg
    10981 atcgtaaacg tagtagctga ataattcgtt attcaacgag acgtaaaaac gccgaccatt
    11041 ggtcggcgtt ttgctttcta tttttcgtca ggcattaatt tcgcaagtag atccattcac
    11101 cattctcttg tgtccataag tcaggatggt ttgctggcgt tagatgcata cgatcactcc
    11161 ttatgtctcg gtcctgcggc actcgctcga aacctaaacc cgagtagatt gcggctgaat
    11221 ttgataagga acgtagacca attttcaaat catggggtaa atcagaatac gcgtttagca
    11281 atgcagcaat gatgaatccg agggcattag aaacgcctgt aacagtgtga agttggtcga
    11341 tataaagatc gattgttggt agttcagatg agcagcaatt aggcatttgg gcaggaaggg
    11401 ttacaacgta tcctatcgtt tggccggtat agtctggagt actataacca ggtcccaaaa
    11461 caacctcgtg tattgtgtat ttactgttgg gttcgaagga ttcgacatct tgcaataaat
    11521 atttcagttc tgcggttaac ctcgcacccg cgtaagcttt attgtatttc tgggcgtgaa
    11581 tattatctaa tgtagcttca tggttccatc caaaagaatt atcaccattc attgttactg
    11641 aatcgccacg ggtttaacag acacctcaga gtcatttaag atgg
//
LOCUS       DAJTRD010000112        11655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTRD010000112 DAJTRD010000000
VERSION     DAJTRD010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.112"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            597..794
                     /locus_tag="OO315_003419"
     CDS             597..794
                     /locus_tag="OO315_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892205.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(744..1937)
                     /locus_tag="OO315_003420"
     CDS             complement(744..1937)
                     /locus_tag="OO315_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5892206.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2073..3797
                     /gene="iucA"
                     /locus_tag="OO315_003421"
     CDS             2073..3797
                     /gene="iucA"
                     /locus_tag="OO315_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5892207.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3798..4745
                     /gene="iucB"
                     /locus_tag="OO315_003422"
     CDS             3798..4745
                     /gene="iucB"
                     /locus_tag="OO315_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5892208.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4745..6487
                     /gene="iucC"
                     /locus_tag="OO315_003423"
     CDS             4745..6487
                     /gene="iucC"
                     /locus_tag="OO315_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5892209.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6484..7821
                     /gene="iucD"
                     /locus_tag="OO315_003424"
     CDS             6484..7821
                     /gene="iucD"
                     /locus_tag="OO315_003424"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5892210.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7827..10022
                     /gene="iutA"
                     /locus_tag="OO315_003425"
     CDS             7827..10022
                     /gene="iutA"
                     /locus_tag="OO315_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5892211.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10573..10716)
                     /locus_tag="OO315_003426"
     CDS             complement(10573..10716)
                     /locus_tag="OO315_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892212.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10955..>11655
                     /locus_tag="OO315_003427"
     CDS             10955..>11655
                     /locus_tag="OO315_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5892213.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEVTQW"
BASE COUNT         2956 a         2897 c         2992 g         2810 t
ORIGIN      
        1 ggagtgaaac aaaggcagct tatcgttgtt tttcaaacga taaagtctct gctaataaaa
       61 tcatgactcc tcacaaagaa aatatcactg aaagagctca gcagtttaaa agagttttag
      121 ttttacagga tacaacagaa cttaattatt ctggtcagaa agaaaaacaa ggagttggtc
      181 ccaaaaaaca taaagatgaa agaaacttat ttcttcatcc tcaactcgtc atcagtgagt
      241 caggtgtttg cctcggtgtt tatgatgact atcagtggtt cagggatgaa ttaaaaacca
      301 gtaaaaatac ccgacaagaa atctgcaatg atttacttca taaaaaacat gtcagtgaaa
      361 aagaaacctg gcgttgggtt gaaggatata ataaagcaac agaactggct cgccaatgcc
      421 ctgataccca tgtattgtca atttctgatc gtgaaggcga tttttatgac ctgtttgaac
      481 gcgcagcgca gactcccgga ataaaagctg actggcttgt cagaatgaaa tttaataaca
      541 gagcaacgct gaatattaat ggtaaacggg atcataggtt gttgcacgaa agaatcatgg
      601 agattacacc gcagcaactg gttgaattca ctatacctga tgggcggggg cagcaagcca
      661 gaacactgcc tgacatgctt tcatcagacg caggtcctct ctcatatcct ctgatttact
      721 ccagtaaggt gatgatcata tctttatttg atagtacggt aatgccagat aaatacagac
      781 acaatggtaa ataatatagt tgcaaataat gctgccataa tcagcgcgat tgcgacacct
      841 gttttcgata ccagcggcac cagcaaagcg cacagtccat agccaagtgt gtgactggta
      901 gcgagccagc ccgcgcctgc gccatcagcc agcttatcat tcagtagaag ttgataagcg
      961 ggtgttgcca gagctgctcc aaatgacagc actgcacagc ccgggtaaaa caaccatagc
     1021 tgttcggaga gcatgatagc cagaccacca ctcatcaaca ctccggcact caggagcagg
     1081 gccaccggag tcaggcgctg cggacggagt accccgaact gcgcgataag cgcagctact
     1141 gcggacagac ccaacagcca ggccacctgt tggctaatgg cggtggtatc cgtagcgaac
     1201 tggcgagtaa gggcaggcga aagtccaagc tgcatcatgc tcaccgccgc agccagtaat
     1261 attgcgcaaa gcagataagg cagacaatcc cgcttcagac tgacgctctt gcactccggt
     1321 gtgggctgtg gtggtgtgcc gggcaaccgc agcagcatca gcagcgccag cactggggct
     1381 gccatcagca gccccagtgg cgccagagga tgaatggcca gcattgctgc cgcgcacagc
     1441 ggcccgaata gcctgccgca actcaggccg gagctgatgg ttgccagagc ggccatgcga
     1501 ttcccttctc cagctctctg caatgcccag acctgacagg ctggcaccat tgccgacacg
     1561 gtcagcccgt aggcgatccg ggcgatgatt aatattccca gtcccaccgc tgttgttatg
     1621 gcgctggtag ccatcagcac gcttcccagc cctagcaggg taaagcttat gccatatcct
     1681 cccagagccc acaacaccac aggtttactg ccagtacggg aaatttgctt tccccaccac
     1741 ggggacgatg gcagaaacag cattgagcca agcatcagca gggcagccca tacagacaga
     1801 ctcagatttg tctgtatgac cagaacaggg agcacaacca gcagaccgtt ctgtccgata
     1861 ccgagaagcc cggcactgaa cgcaagtggc cagcaggaca gtggtttttg gggcgtatct
     1921 tcgatccctg gtgacattat aatgtttcaa ctccatgtat taattgtgtt tatttgtaaa
     1981 attaatttat ctgacaataa catttattgt tgataatgag aatcattatt gacataattg
     2041 ttattatttt actgtgtggg agctgtttga ctatgaccct gccctctgaa aaaccagcca
     2101 cagatgtggc tgcgcagtgc ttcctgaatg cactgattcg tgaaaccaca gactggaaac
     2161 tgacagaata cccgccagac gaattgctca tcccgctgga tgagcagaaa tcgctccatt
     2221 tcagagtggc ttatttctcc ccaacccagc atcaccgctt tgcatttcct gcacgtctgg
     2281 tcacggcatc aggcagttat cctgtcgact ttaccactct ctcccggctt attattgata
     2341 agctacgcca tcaactgttt ctgcccgttc ccctctgcga aactttccac cagcgcgtgc
     2401 tggaaagcca tgtccatacg caacaggcaa ttgatgcccg tcatgactgg gccgctctgc
     2461 gtgaaaaagc gttgaatttt ggcgaggctg agcaggcgct gctgacagga cacgctttcc
     2521 accctgcgcc taagtctcat gaaccgttta accggcggga ggctgaacgc tacctgcctg
     2581 atatggcacc ccacttccca ctgcggtggt tttcggtgga taaaacgcaa atcgccggtg
     2641 aaagtttgca tctcaacctt caacagcggc tgacgcgatt tgccgcagag aatgcgcctc
     2701 agttactcaa cgaattaagt gacaaccaat ggctgttccc gttgcacccg tggcaggggg
     2761 aatatctttt gcagcagggg tggtgccagg cacttgttgc taaagggctg attaaagact
     2821 taggtgaggc cggcacgtcg tggctgccga ccacctcttc ccgttccctc tactgtgcca
     2881 ccagccgcga tatgatcaag ttctccctga gcgtacggct gaccaactcc atccgtactc
     2941 tgtccgtgaa agaagtgaag cgtgggatgc gcctggcacg cctggctcaa accgacggct
     3001 ggcagatgct acaggtccgc ttcccgactt tccgggtaat gcaggaggat ggctgggccg
     3061 ggctgctcga tcttaacggc aacatcatgc aggaaagtct gtttgccctg cgtgaaaatc
     3121 tgctggtgga tcagccgaaa agccagacta acgtactggt ctccctgact caggccgcac
     3181 ctgatggcgg tgattcgctg ctggtttcgg cggtaaaacg cctgagcgat cgcctcggta
     3241 tcactgtgca acaggccgcc catgcatggg ttgatgcata ctgtcagcaa gtgctaaagc
     3301 cgctgtttac ggctgaagcg gattacggcc tggtgctgct ggcgcatcag caaaatattc
     3361 ttgtccagat gcttggggat ctgccggtcg gatttattta ccgtgactgt cagggcagcg
     3421 cttttatgcc tcacgcgaca gactggctcg attctattgg cgaggcgcag gcggaaaata
     3481 ttttcaccca tgagcagttg ctgcgctatt tcccttatta cctgctggtt aactccactt
     3541 ttgctgtgac cgctgcgctg ggggctgccg ggctggacag cgaatcgaat ctgatggctc
     3601 gtgtacgagc atcgctggct gaagtgcgtg atcaggtgac tcataaaacc tgtctcaact
     3661 acgtgctgga aagtccgtac tggaacgtaa aaggtaactt tttctgttat ctgaacgatc
     3721 ataacgagaa caccattgtt gacccttcgg tgatctattt cgatttcgct aacccgctgc
     3781 aggctcagga ggtctgaatg tctgaggcaa acattattca cagcagatat ggactgcgct
     3841 gtgaaaaact cgacaagccc ctgaatcttg gctggggact ggacaatagc gcggtgttgc
     3901 actgtcccgg ggagctgccg acagggtggc tgtgcgatgc gctggatcag atatttatcg
     3961 ccgcaccaca actgtcagca gtggctctcc cctgggccga atggcgtgag gagccacagg
     4021 cgctgacgct ttttgggcag gtaaaaagcg acattatcca tcgtactgcc ttctggcagt
     4081 taccgttatg gttgagttca ccggcaaacc gggcctccgg tgaaatggtt tttgatgcag
     4141 agcgtgagat ttatttcccg cagcgcccac cccgtccaca gggtgaggtt taccgccgtt
     4201 acgatccacg tattcgcagg atgctgagtt tccgcattgc cgatcccgtt tctgatgcag
     4261 aacgtttcac ccgctggatg aatgatccgc gcgttgagta tttctgggag caaagtggct
     4321 cactggaggt acagaccgcc tatctggagc gccagctcac cggtaaacat gcattcccgc
     4381 tgattgggtg cttcgacgat cggccattca gctatttcga aatctactgg gcggcggaag
     4441 accgcattgg tcgtcactat tcgtggcagc cctttgaccg tggcctgcat ctgctggttg
     4501 gtgaacagca atggcgcggg gcccactatg tgcaaagctg gctgcgcggg ctgacacatt
     4561 acctgctgct ggatgagccc cgtacgcagc gcacagtact ggagccacgc accgataacc
     4621 agcgcctgtt ccgccatctt gagcctgcgg gataccggac aattaaagag ttcgacttcc
     4681 cacataagcg ctcgcgcatg gtgatggcgg atcgtcatca cttcttcacg gaggtcggtc
     4741 tgtaatgaat cacaaggact gggattttgt taaccgtcgg ctggtggcaa aaatgttgtc
     4801 tgagatggag tatgagcagg ttttccacgc ggaatctcag ggcgatgacc actactgcat
     4861 taacctgccg ggagcacaat ggcgcttcat cgctgaacgt ggtatctggg gctggctgtg
     4921 gattgatgcc caaacgttgc gctgcacgga cgaaccagta ctggcgcaga cgctgctgat
     4981 gcagctaaag ccggtgctgt caatgagtga tgccactgtt gcagagcata tgcaggattt
     5041 gtatgccacg ctactgggcg acctgcaact gctgaaggcc cgtcgcggac tgagtgccag
     5101 tgacctgatt aatcttgatg ccgatcgtct gcaatgcctg ctgagcggtc atcctaaatt
     5161 cgtttttaat aaaggtcgcc gtggctgggg caaagaggcg ctggaacgat atgcgccaga
     5221 gtataccaac accttcagac tgcactggct ggcggtaaaa cgtgaacata tgatctggcg
     5281 ctgcgataac gatctggata ttcagcagtt gttgactgcc gcaatggatc cgcaggagtt
     5341 tacacgcttc agtcaggtct ggcaggaaaa cgggctggat cacaactggc tgccgctgcc
     5401 ggtacatccg tggcagtggc agcaaaaaat cgccacagac ttcatcgctg attttgccga
     5461 aggcaggatg gtgtctctcg gcgagtttgg cgaccagtgg ctcgcccagc agtcgctgcg
     5521 taccctgacc aacgccagcc gccggggagg gctggatatc aagctgccgc tgaccatcta
     5581 caatacctca tgctaccggg ggatccctgg cagatatatc gctgccggac cactggcttc
     5641 acgctggctg caacaggttt ttgcgaccga cgccaccctg gtgcaaagcg gcgcagtgat
     5701 ccttggtgaa ccggctgcag gctatgtgtc ccatgaaggc tatgccgcgc ttgcccgggc
     5761 tccctatcgc taccaggaaa tgcttggtgt tatctggcgg gagaatccgt gccgctggct
     5821 gaaaccggat gaaagtcctg ttctgatggc aacactgatg gagtgcgacg aaaacaatca
     5881 gccgctggca ggcgcatata tcgaccgctc cggactggac gctgaaacct ggcttacgca
     5941 attgttccgg gtggttgtgg ttccgctgta tcacctgctt tgccgctacg gtgtcgcgct
     6001 tattgcacac ggacaaaata taacgctcgc catgaaagag ggggtaccac agcgtgttct
     6061 gctgaaagac ttccagggcg atatgcggct ggtgaaagaa gcgtttcccg aaatggactc
     6121 tttgcctcag gaggtgcgtg atgtcacatc ccgtctgagt gcggactact taatccatga
     6181 tttgcagacg ggtcacttcg tgacagtact gcgttttatt tcgccactga tggttcgtct
     6241 tggcgtacct gaaaggcgat tttatcaact gctggcagca gtgttgagtg attacatgaa
     6301 caaacatcca caaatggcag agcgttttgc gcttttctca ctcttcaggc cacaaatcat
     6361 tcgcgtggtg ctgaacccgg taaaactgac ctggccggac ctggatggcg gcagtcgcat
     6421 gctgccgaat taccttgaga atctgcaaaa tccgctgtgg ctggtaactc aggaatatga
     6481 atcatgaaaa aaagtgtcga ttttattggt gtcgggacag ggccatttaa tctcagcatt
     6541 gctgcattgt cacatcagat cgaagaactg aactgtctct tctttgacga acatcctcat
     6601 ttttcctggc atccgggtat gctggtaccg gattgtcata tgcagaccgt ctttctgaaa
     6661 gatctggtca gtgcagttgc acctacaaat ccctacagct ttgttaacta tctggtgaag
     6721 cacaaaaagt tctatcgctt ccttacaagc aggctacgta cagtatcccg tgaagagttt
     6781 tctgactatc tccgctgggc tgctgaagat atgaataacc tgtatttcag tcataccgtt
     6841 gaaaatattg atttcgacaa aaaaagtcga ttgtttctgg ttcagaccag tcggggagaa
     6901 tattttgccc acaatatctg ccttggtaca ggaaaacaac cttatttacc accctgtgtg
     6961 aagcatgtga cacaatcctg tttccatgcc agtgaaatga atcttcgtcg gccggacctg
     7021 agtggaaaac ggataaccgt ggttggtgga ggacagagtg gtgcagacct gttccttaat
     7081 gcattacgcg gggaatgggg agaagcggcg gaaataaact gggtctcacg gcgtaataat
     7141 tttaacgcac tggatgaggc tgcttttgct gatgagtatt ttacacctga atatatttca
     7201 ggcttctccg gactgaagga agatattcgc catcagttac tggatgagca gaaaatgaca
     7261 tcggatggca tcactgccga ttctttactg accatttatc gtgagttgta ccaccgtttt
     7321 gaagttctga gaaaaccaag aaatatccgt ctgctaccca gtcgctcggt aacaactctg
     7381 gaaagtagtg gtccgggctg gaggttattg atggaacatc atctggatca gggcagagag
     7441 agcctggaaa gtgatgtggt gattttcgcc acaggttacc gttctgcatt gccacaaata
     7501 cttccctcac tgatgccctt gatcaccatg cacgataaga acacctttaa agtgcgtgat
     7561 gacttcactc tggaatggag tggcccgaaa gaaaataaca tctttgcggt caacgccagc
     7621 atgcaaactc atggcatcgc cgaaccccag ctcagcctga tggcctggcg atctgcacgt
     7681 attcttaatc gcgtactggg gcgtgattta ttcgatctca gtatgccgcc cgcactgatt
     7741 cagtggcgca gcggcagccg gaaaaaacca cagccggagg ctgcttcttt aactcactat
     7801 acagcaaata ttcaggaata acgatgatgc gcaaaaagta tatgccccgg gctcttggtc
     7861 cgctgcttct tgtcgtgctg tcaccagctg tcgcccagca aaacgatgat aatgagatca
     7921 tagtgtctgc cagccgcagc aatcgaactg tagcggagat ggcgcaaacc acctgggtta
     7981 tcgaaaatgc cgaactggag cagcagattc agggcggtaa agagctgaaa gacgcactgg
     8041 ctcagttaat ccccggcctt gatgtcagca gccagagtcg aaccaactac ggtatgaaca
     8101 tgcgtggccg cccgctggtt gtcctgattg acggtgtgcg cctcaactct tcacgttccg
     8161 acagccgaca actggactct gtcgatcctt ttaatatcga ccatattgaa gtgatctccg
     8221 gcgcgacggc cctgtacggt ggcgggagta ccggagggtt gatcaacatc gtgaccaaaa
     8281 aaggtcagcc ggaaaccatg atggagtttg aggctggcac aaaaagtggc tttaacagca
     8341 gtaaagatca cgatgagcgc attgccggtg ctgtctccgg cggaaatgac catatctccg
     8401 gacgtctttc cgtggcatat cagaaatttg gcggctggtt tgacggtaac ggcgatgcca
     8461 ccctgcttga taacacccag accggcctgc agcactccaa tcggctggac atcatgggaa
     8521 ccggtacgct gaacatcgat gaatcccggc agcttcaact gataacgcag tactataaaa
     8581 gtcagggaga cgacaattac gggcttaatc tcgggaaagg cttttccgcc atcagcggga
     8641 gcagcacacc atacgtcagt aaggggctga attctgaccg cattcccggc actgagcggc
     8701 atttgatcag cctgcagtac tctgacagtg atttcctggg acaggaactg gtcggtcagg
     8761 tttactaccg cgatgagtcg ttgcggtact acccgttccc gacggtaaat gcgaataaac
     8821 aggcgacggc tttctcctcg tcacagcagg ataccgacca gtacggcatg aaactgactc
     8881 tgaacagcca actgatggac ggctggcaaa tcacctgggg gctggatgct gagcatgagc
     8941 gctttacctc caaccagatg ttcttcgatc tggctcaggc aagtgcttcc ggagggctga
     9001 acaaccataa gatttacacc accgggcgct atccgtcata tgacatcacc aatctggcgg
     9061 ccttcctgca atccagctat gacattaatg atatttttac cgttagcggt ggcgtacgct
     9121 atcagtatac tgagaacagg gtagatgatt tcatcgacta cacgcagcaa cagaagattg
     9181 ctaccgggaa ggcgatatct gccgacgcca ttcctggtgg ttcggtagat tacgataact
     9241 ttctgttcaa tgctggtctg ctgatgcaca tcaccgaacg tcagcaggca tggttcaatt
     9301 tttcccaggg ggtggcattg ccggatccgg ggaaatatta tggtcgcggc atctatggtg
     9361 cagcagtgaa cggccatctt cccctgacaa agagcgtgaa cgtcagcgac agtaagctgg
     9421 aaggcgtgaa agtcgattct tatgaactgg gctggcgctt tatcggtgac aacctgcgga
     9481 ctcaaatcgc ggcatattac tcgctttcca ataagagcgt ggaaaggaat aaagatctga
     9541 ccatcagtgt gaaggacgac aggcgccgta tttacggcgt ggaaggtgcg gtggactacc
     9601 tgatcccgga tactgactgg agtaccggtg tgaacttcaa tgtgctgaaa accgagtcga
     9661 aagtgaacgg tcaatggcaa aaatatgacg tgaaggaatc aagtccatcg aaagcgacag
     9721 cttacattaa ctgggcgccg gaaccgtgga gtctgcgtgt acagagcacc acttctttcg
     9781 acgtaagcga tgcagagggt aacgatatta atggttacac taccgtcgat tttatcagta
     9841 gttggcagct tccggtggga acactcagct tcagcgttga gaacctcttc gaccgtgact
     9901 ataccactgt ctggggacag cgtgcacctc tgtactacag cccgggttac ggccctgctt
     9961 cactgtacga ctacaaaggc cggggccgaa cctttggtct gaactactca gtgctgttct
    10021 gacggggatg cttttgcaac aagggtatgc tgtaaacaac atggtccttg gtggccatgt
    10081 tgtttacagc cagtataata tacagcctta acaagatatt ccgtctccgg aatgggctgt
    10141 tcaccactga ataacattat gtctgtttta tggatctttg actgaaattt atcgcatatc
    10201 ctcatttcat tgtccgtctg aaaactaacc ttacaagtaa gcgtcgtctc agcaccgtct
    10261 ggcagatcct gaaattcctg agaggatgat ctgcgcccaa tatcctgagc cggaactttg
    10321 ctgcaatgaa ggccaacgaa aaatggggga gtatgttata gagtacttgc tacagggtaa
    10381 aaagaggtac ctgtcgctgg ttctccgttc agtttaaccg ggagattttc tcctgcgcct
    10441 gtcataaagt ccggtaatgg agattgttaa tacgatggtg cacgatgcat cgtcagtgcc
    10501 cggcccggag gatgccccgt agctacatac ggatcagggt tggcaatatc gaatggcagg
    10561 ctataggcaa agttaaagcc ccatggagta gcacaaaata tgccgcacaa aggaaacggt
    10621 ctgaataacg cagtgatgga gaacttcttc ggcacactgc aaaatcgcat cgtggtcgat
    10681 cgctaattcg tcgtgaatca ctgaacgatg ttccatgcga acagatattc gatcatatcg
    10741 aaatattaaa cattacccct ccccacattg ggcaaaaaaa aacaaaaaac gatcaattag
    10801 tattgattca cataatctct catcattaat gagaacattg tcacatattt acaatataga
    10861 actgttactt ttagcaagct gttagtaacc aaccgttact aataatttta attcttaata
    10921 ctttgttcat tcaataaatg agttgagaga atatatgaat aaaatatact cccttaaata
    10981 tagtgctgcc actggcggac tcattgctgt ttctgaatta gcgaaaagag tttctggtaa
    11041 aacaaaccga aaacttgtag caacaatgtt gtctctggct gttgcaggta cagtaaatgc
    11101 agcaaatatt gatatatcaa atgtatgggc gagagactat cttgatcttg cacaaaataa
    11161 aggtattttc cagcccggag caacagacgt aacaatcact ttaaaaaacg gagataaatt
    11221 ctctttccat aatctctcaa ttccggattt ttctggtgca gcagcgagtg gcgcagctac
    11281 cgcaatagga ggttcttata gtgttactgt tgcacataac aaaaagaacc ctcaggccgc
    11341 agaaactcag gtttacgctc agtcttctta caaggttgtt gacagaagaa attccaatga
    11401 ttttgagatt cagaggttaa ataaatttgt tgtggaaaca gtaggtgcca ccccggcaga
    11461 gaccaaccct acaacatatt ctgatgcatt agaacgctac ggtatagtca cttctgacgg
    11521 ttcaaaaaaa atcataggtt ttcgtgctgg ctctggagga acatcattta ttaatggtga
    11581 atccaaaatc tcaacaaatt cagcatatag ccatgacctg ttaagtgcta gtctatttga
    11641 ggtcacccaa tggga
//
LOCUS       DAJTRD010000113        11580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTRD010000113 DAJTRD010000000
VERSION     DAJTRD010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.113"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(272..347)
                     /locus_tag="OO315_003428"
     tRNA            complement(272..347)
                     /locus_tag="OO315_003428"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(312..314),aa:Ile,seq:cat)
     gene            906..3158
                     /locus_tag="OO315_003429"
     CDS             906..3158
                     /locus_tag="OO315_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892214.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3494..4426
                     /gene="csiD"
                     /locus_tag="OO315_003430"
     CDS             3494..4426
                     /gene="csiD"
                     /locus_tag="OO315_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5892215.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4446..5714
                     /gene="lhgO"
                     /locus_tag="OO315_003431"
     CDS             4446..5714
                     /gene="lhgO"
                     /locus_tag="OO315_003431"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5892216.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5737..7185
                     /gene="gabD"
                     /locus_tag="OO315_003432"
                     /pseudo
     CDS             5737..7185
                     /gene="gabD"
                     /locus_tag="OO315_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7193..8479
                     /gene="gabT"
                     /locus_tag="OO315_003433"
                     /pseudo
     CDS             7193..8479
                     /gene="gabT"
                     /locus_tag="OO315_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8717..10117
                     /gene="gabP"
                     /locus_tag="OO315_003434"
     CDS             8717..10117
                     /gene="gabP"
                     /locus_tag="OO315_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5892217.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10138..10800
                     /gene="csiR"
                     /locus_tag="OO315_003435"
     CDS             10138..10800
                     /gene="csiR"
                     /locus_tag="OO315_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5892218.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10801..11250)
                     /gene="kbp"
                     /locus_tag="OO315_003436"
     CDS             complement(10801..11250)
                     /gene="kbp"
                     /locus_tag="OO315_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5892219.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11334..11492)
                     /locus_tag="OO315_003437"
     CDS             complement(11334..11492)
                     /locus_tag="OO315_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5892220.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2977 a         2844 c         2887 g         2872 t
ORIGIN      
        1 tttgaaaacc agaacctcgc ttaggcctgt gtccatatta cgtgggtagg atcaatttgc
       61 ccgcttggca tagcgggcat tttttccagg tacttttgaa tgagtactga tgaataaata
      121 cattgcagtg gcgtgccacg taccaaaaca ccagccctca ttcgaaacca cccaccgcac
      181 ttcttccttg aaatggcgtt agtcatgaaa tatagaccgc catcgagtac cccttgtacc
      241 cttaactctt cctgatacat gaatgatgat ttggtggccc ttgctggact tgaaccagcg
      301 accaagcgat tatgagtcgc ctgctctaac cactgagcta aagggcctta agtatgcaat
      361 aacaatactt ataaaccacg caataaacat gatgatctta tgatgtaaat aacagatttt
      421 tatgcgttcc cattcacctt gggtcgtgat tacacgcgac ataaaacccg acaccgcctc
      481 cattcgcaaa gtcgatactc gcagtcaaca agcaaatgtt aataattagc actatctata
      541 gttatcatcg attcaatgat actttgtaat gattttgtat ctaataatat aactttatta
      601 cattagctga agagttttcg catcattatg atatctgtta cttttcactc cataaaaata
      661 aacttcatat agcaatatat tctttcatag atcttattct gctaatcatt agtttcgtat
      721 gcgcgatttt tgacagttgc ctctccagac cacatcgata attaaaaaac agatttaaac
      781 attatccttt tccatataga tattggataa aaataggaca tctgtttgca attactttca
      841 caacaattaa acatttttat gttttcgtat acatcatatt actctaccat tagaggaacg
      901 ttattatgtt ttctataaaa ccaggacctg gaaatttacc tatcgacaac cccacattgt
      961 tatcatggaa cattactgat ggggatctaa attccaaatt taatacatta gaatatctaa
     1021 actgtataac aaatattatt aatgcttgtg gagtttatcc tcaggattta aaagacagag
     1081 aaattatatc aacttttcac gcagaaaaag tcattaatga tctgttaaaa aacgattata
     1141 aaatttccct ttctccagat acaacttacc gagagctgaa taaagctgca cagagtagca
     1201 ttacagcgcc agacaggata ggagaaggga aaacatgggt ttatcaacga gatacaatgg
     1261 ttgaaagagg tgataacagt ggtgttcatc agtatggtcc agctgaacat ttcactcaca
     1321 ttatatctga caaaccgtcc ccaaaagata agtatgttgc atatgctatt aacattcctg
     1381 actatgagct agcagccgat gtatataata ttaacgtgac gtcaccttcc ggacagcaag
     1441 aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag cgtaaatctc
     1501 ttactgctgt tcagaaatca caatgtgaaa tcatcacccc caaaaaacct ggcgaagcga
     1561 ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct gagtttgcac
     1621 gttcccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc gacggaccag
     1681 aaactcccga agaagaaaaa ggttactggt ttcacgctta tcaacccgaa gatctttgta
     1741 ccatccataa tccaatgggg gatttgcagg attttatcgc attggttaaa gatgctaaaa
     1801 aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatc gggggaagtg
     1861 gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt aaagatatag
     1921 aaagtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt aaagatccag
     1981 taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca aaagacttcg
     2041 aagcaagtaa agataacatc tctaaggatg aatggaaaaa cctccatgca ttaaaagaaa
     2101 agagtttaaa tggaatgcct aaaacaaccc ccaaaagtga ccaggttatt atgttgcaaa
     2161 atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttat gatgcagcaa
     2221 aacactcaaa acatgaacaa atagaaagat caataacccc accgcttaaa aattataatg
     2281 agcgattaca caatactaac ttatttaacc caaaatatca tgaaaaaacc gttatgaatt
     2341 acatggaata tctggtaact tgtcagttga atgaagaaca aatgtcatcg ctgctttatg
     2401 aaagagatga tttaagcgcc attgattttt cattgctcat gaagacgata aaagcctttt
     2461 cattcggggg agatctccta acccttgcat caaaaccggg ttccacaatc tcaagcatcc
     2521 catcagaaag acggatattg attaacatta accacgattt tcctaacaat ggcaatcttt
     2581 tcaatgactt tctatttaac catcaacaag atgaacaatt agcaatggca tatatggccg
     2641 ctctcccgtt cagcaggcct ttagtttact gggatggcca ggtattaaaa tcaacgactg
     2701 aaattaaaaa ttatgatggg cccacgcgtg tcggtggtga ggcgtggctt aataaaggtt
     2761 gctctaccta tcagcagctc tacaatgaat tccacgcatt atatatagat aaagcaggaa
     2821 tatggagcgc atttgagggt gtatttgcaa ctaaaaacgt tctggccttt agtcgtgggg
     2881 attctgtgaa cattaatcac tctcctcatg atggactagt tataataaat aaaggaaacg
     2941 aagaagttga aggtacctgg cctaacaaat tgcaacctgg aatatataaa aacatgggga
     3001 gtaatagcgt taacattatt attaataata cccgaaaaat tatcccccct tgtaaagtat
     3061 ttacgcttag aggcggatct ctaaatatca atattcctag gcgtagcgct cttcttttag
     3121 ggaaaacagg agaaccgccg aactatctct atttataatt taatgttata tctgccccga
     3181 taaaacgggg cagataatat gtttagttta ctaacggtca ttttgtagtg aagccattta
     3241 ctgtttttta tcgaccagat aatctgttct ctaatgttaa ctccccctaa cctgttgctt
     3301 tagttattca tttcctgtct cactttgcct taatacccta cgttaaatgt tactaatttg
     3361 ttgcttttga tcacaataag aaagcaatat gtcgcttttg tgcgcatttt tcagaaatgt
     3421 agatattttt agattatggc tacgaaatga gcatcgccat gtcaccctac atctcataag
     3481 aggatcgctt ctgatgaatg cactgaccgc cgtacaaaat aacgctgtcg attcaggcca
     3541 ggactatagc ggattcactc tcatcccgtc ggcgcaatcc ccgcgtctgc tggaactcac
     3601 cttcaccgaa cagacgacca aacagtttct cgaacaggtt gccgaatggc ccgtgcaggc
     3661 gctggagtac aaatcgtttc tgcgttttcg ggtaggcaaa attcttgacg atctgtgtgc
     3721 gaatcagctg caaccattgc tgttgaagac cctgctaaac cgtgctgaag gtgcgctgtt
     3781 gatcaatgcg gtaggtatcg atgatgtggc gcaggcagat gagatggtga agctggcaac
     3841 ggcggtagcg catctgattg gtcgctcaaa tttcgatgcg atgagcggtc agtattacgc
     3901 gcgtttcgtg gtgaaaaatg tcgataactc agacagctat ctgcgtcagc cgcaccgcgt
     3961 gatggaactg cacaacgacg gcacgtatgt cgaagagatc accgattacg tgctgatgat
     4021 gaaaatcgat gactgggaac atctggacca ctatttccgc catccactgg cgcgtcgccc
     4081 gatgcgcttt gccgcgccgc cgagcaaaaa cgtcagcaaa gatgttttcc atccagtgtt
     4141 cgacgttgat caacagggtc gcccggtgat gcgctatatc gaccagttcg tccagccaaa
     4201 agattttgaa gaaggcgtgt ggttgagcga gctttccgat gccattgaaa ccagcaaagg
     4261 cattctttct gtacccgttc ccgttggcaa attcctgttg ataaacaacc tgttctggct
     4321 gcacggtcgc gaccgcttta ctccgcaccc ggatctgcgc cgtgaactga tgcgtcagcg
     4381 tggctatttc gcttacgcca ctcaccacta ccagacgcat cagtaagcgc aaaggaattg
     4441 agcggatgta tgattttgtg attattggcg gcggcatcat cggcatgtcg accgccatgc
     4501 aactgattga tgtttatccg gatgcccgca ttgcgttgct ggaaaaagag tccggcccgg
     4561 cctgtcacca gacgggccac aacagcggcg tgatccatgc cggggtctat tacacgcccg
     4621 gcagcctgaa ggcgcagttt tgcctggcgg ggaaccgtgc caccaaagcc ttttgcgatc
     4681 aaaacggcat tcgctatgac aactgcggca agatgctggt cgccacgtct cagctcgaaa
     4741 tggaacggat gcgcgcctta tgggaacgca cagcggcgaa cggtatcgag cgcgagtgga
     4801 taaacgccgt ggaactgcgc gagcgcgaac cgaatatcac cgggcttggt ggcatttttg
     4861 tgccgtccag cggcattgtc agctaccgcg aagtgacggc ggcaatggca aaaatcttcc
     4921 aggccagagg cggcgagatt atttataacg ccgaagtcag cgccctcagt gagcataaaa
     4981 acggcgtggt gatacgcacc cgtcagggcg gtgaatatga agcatcgacg ctgattagct
     5041 gttccgggct gatggctgac cggctggtga aaatgctcgg cctcgaaccg ggctttatta
     5101 tctgcccgtt ccgtggcgag tatttccgcc ttgcgccgga gcataaccag attgttaacc
     5161 acctgattta ccccattccc gaccccgcga tgccattttt gggcgtgcat ctcacccgca
     5221 tgatcgacgg cagcgtgacc gtcgggccaa acgcggtgct ggctttcaaa cgcgaaggct
     5281 atcgcaagcg cgacttctca tttagtgaca cgctggagat tttgagctcg tcggggattc
     5341 gccgggtgct gcaaaaccat ctacgctcag gactgggcga gatgaaaaac tcgctgtgca
     5401 aaagcggcta tctgcggctg gtgcaaaagt attgtccccg gctttcgtta agcgatctcc
     5461 agccctggcc cgccggtgtg cgggcgcagg cagtatcgcc ggacggcaag ctgattgacg
     5521 attttctgtt tgtcaccacc ccacgcacga tccacacctg caatgcgccc tccccggcag
     5581 cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa ggtacaaacg ctgttggcaa
     5641 gccagagtaa ccccggacgc acgctgcgag cggcacgtag tgtggatgcc ttacacgccg
     5701 catttaatca ataacctttg aaaacaggac gtaacaatga aacttaacga cagtaactta
     5761 ttccgccagc aggcgttgat taacggggaa tggctggacg ccaacaatgg tgaagtcatc
     5821 gacgtcacca atccggcgaa cggcgacaag ctgggtagcg tgccgaaaat gggcgcagat
     5881 gaaacccgcg ccgctatcga cgccgccaac cgcgccctgc ccgcctggcg tgcgctcacc
     5941 gccaaagaac gcgccaccat tctgcgcaac tggttcaatt tgatgatgca gcatcaggac
     6001 gatttagcgc gcctgatgac cctcgaacag ggtaaaccgc tggctgaagc gaaaggcgaa
     6061 atcagctacg ccgcctcctt cattgagtgg tttgctgaag aaggcaaacg tatttatggc
     6121 gacaccattc ctggtcatca ggccgataag cgcctgattg ttatcaagca gccgattggc
     6181 gtcaccgccg ccatcacgcc gtggaacttt ccggcggcga tgattacccg taaagccggt
     6241 ccggcgctgg cggcaggctg caccatggtg ctgaagcccg ccagtcagac gccgttctct
     6301 gcgctggcgc tggcggaact ggcgattcgc gcgggcattc cggctggcgt gtttaacgtg
     6361 gtcaccggtt cggcaggcgc ggtcggtaat gaactgacca gcaacccgct ggtacgcaaa
     6421 ctgtcgttta ccggttagac cgagattggc cgccagttaa tggaacagtg cgcgaaagac
     6481 atcaaaaaag tgtcgctgga gctgggcggc aacgcgccgt ttatcgtctt tgacgatgcc
     6541 gatctcgata aagccgtgga aggcgcgctg gcctcgaaat tccgcaacgc cgggcaaacc
     6601 tgcgtctgcg ccaaccgttt atacgtgcag gacggcgtgt atgaccgctt tgccgaaaaa
     6661 ttacagcagg cggggagcaa actgcacatc ggcgacgggc tggagaaagg cgtcaccatc
     6721 gggccgctga tcgatgaaaa agcggtagca aaagtggaag agcatattgc cgatgcgctg
     6781 gagaaaggtg cgcgtgtggt ttgcggcggt aaagcgcacg aacgcggcgg caacttcttc
     6841 cagccaacca ttctggtgga cgttccggcc aacgccaaag tgtcgaaaga agagacgttc
     6901 ggccccctcg ccccactgtt ccgttttaaa gatgaagccg atgtgattgc acaagccaat
     6961 gacaccgagt ttggtcttgc cgcctatttc tacgcccgtg atttaagccg cgtcttccgc
     7021 gtcggcgagg cgctggagta cggcatcgtc ggcatcaata ccggcattat ttccaatgaa
     7081 gtggccccat tcggcggcat caaagcctcg ggtctgggtc gtgaaggttc gaagtatggc
     7141 atcgaagatt acttagaaat caaatatatg tgcatcggtc tttaactgga gaatgcgaag
     7201 gaacagcaat aaagagttaa tgcagcgccg cagtcaggcg attccccgtg gcgttgggca
     7261 aattcacccg attttcgctg accgcgcgga aaactgccgg gtgtgggacg tttaaggccg
     7321 tgagtatctt gatttcgcgg gcgggattgc ggtgctcaat accgggcacc tgcatccgaa
     7381 ggtggtggcc gcggtggaag cgcagttgaa aaaactgtcg cacacctgct tccaggtgct
     7441 ggcttacgag ccgtatctgg agctgtgcga gattatgaat cagaaggtgc cgggcgattt
     7501 cgccaagaaa acgctgctgg ttacgaccgg ttccgaagcg gtggaaaacg cggtaaaaat
     7561 cgcccgcgcc gccaccaaac gtagcggcac catcgctttt agcggcgagt atcacgggcg
     7621 cacgcattac acgctggcgc tgaccggcaa ggtgaatccg tactctgcgg gcatggggct
     7681 gatgccgggt catgtttatc gcgcgcttta tccttgcccg ctgcacggca taagcgagga
     7741 tgacgctatc gccagcatcc accggatctt caaaaatgat gccgcgccgg aagatatcgc
     7801 cgccatcatg attgagccgg ttcagggcga aggcggtttc tacgccgcgt cgccagcctt
     7861 tatgcagcgt ttacgcgctc tgtgtgacga gcacgggatc atgctgattg ccgatgaagt
     7921 gcagagcggc gcggggcgta ccggcacgct gtttgcgatg gagcagatgg gcgtggcgcc
     7981 ggatcttacc acctttgcga aatcgatcgc aggcggtttc ccgctggcgg gcgtcaccgg
     8041 gcgcgcggaa gtgatggacg ctgttgcacc gggcggtctg ggcggcacct atgccggtaa
     8101 cccgattgcc tgcgtggctg cgctggaagt gttgaaggtg ttcgagcagg aaaatctgct
     8161 gcaaaaagcc aacgatctgg ggcagaagct aaaagacgga ttgttggcga tagccgaaaa
     8221 acacccggag atcggcgacg tacgcgggct gggatcgatg attgccatcg agctgtttga
     8281 agatggcgat cacagcaaac cggacgcaaa actcaccgcc gagatcgtgg cacgcgcccg
     8341 cgataagggc ctgattcttc tctcctgcgg cccgtattac aacgtgctgc gcatccttgt
     8401 accactcacc attgaagacg ctcagatccg tcagggtctg gagattatcg gtcagtgttt
     8461 tgatgaggcg aagcagtagc accgtcccca tgcaggaggc gacgctgcgc gtcttgtccg
     8521 gcctacgaga atccaagtcg gataaggcgt ttacgccgca tccgacaatc tgtacgtgaa
     8581 caggaataaa tctatgtcgg ccggataagg cggagccgct ctccggcaaa aagaatcaat
     8641 aacaattaaa cgcgtgaccc gacgcgggaa atgtcggggc gctctcccaa gtgacacact
     8701 ttcgagagga ttcaggatgg ggcaatcatc gcaaccacac gagttaggcg gcgggctgaa
     8761 atcacgccac gtcaccatgt tgtctattgc cggtgttatc ggcgcaagtc tgtttgtcgg
     8821 ttccagcgtc gccatcgccg aagcggaccc ggcggtatta ctggcctatc tgttcgccgg
     8881 attactggtg gttatgatta tgcggatgct ggcggaaatg gcagttgcca cgcccgatac
     8941 cggttcgttt tccacctatg ccgataaagc cattggccgc tgggcgggct ataccatcgg
     9001 ctggttgtac tggtggtttt gggtactggt tatcccactg gaagccaaca tcgccgccat
     9061 gatcctgcac tcgtgggttc caggcattcc catctggtta ttttccctcg tcattaccct
     9121 cgccttaact ggcagtaatt tattaagcgt taaaaactac ggcgaatttg agttctggct
     9181 ggcgctgtgc aaagtcatcg ctatcctggc ctttattttc cttggtgcag tcgcaattag
     9241 cggtttttac ccttatgccg aagtgagcgg gatctcaaga ttgtgggata gcggcggctt
     9301 tatgcccaac ggtttcggtg cggtattaag cgcgatgttg atcaccatgt tctcgtttat
     9361 gggcgcagaa attgtcacca ttgccgccgc ggaatccgac atgccggaaa aacatattgt
     9421 ccgcgccacc aactcggtta tctggcgtat ttctatcttc tatttgtgct ctatttttgt
     9481 cgtcgtggca ttaataccgt ggaatatgcc ggggctgaaa gccgttggat cttatcgctc
     9541 ggttctggaa ttgctcaata ttccccatgc gaaattaatc atggactgtg tgatattact
     9601 ttccgtaacc agttgcctga actcggccct gtataccgcc tcaaggatgc tctactcctt
     9661 aagccgtcgc ggtgatgctc ccgcggtaat gggtaaaatc aaccgcagta aaaccccgta
     9721 cgtggcggtg ttactctcca ccggcgcagc attcttaacg gtggtggtga actattacgc
     9781 gcctgcgaag gtatttaaat ttctgatcga cagctccggc gctatcgccc tgctggttta
     9841 tttagtcatc gccgtttcac agttgcggat gcgcaaaatt ctgcgagcag aaggaagcga
     9901 aattcgcttg cgaatgtggc ttgatccctg gctcacctgg ctggtcatcg gctttattac
     9961 ctttgtgttg gtagtgatgc tattccgtcc ggcgcaacag ttagaagtga tctccaccgg
    10021 cttattagcg atagggatta tctgtaccgt gccaattatg gctcgctgga aaaagctggt
    10081 attgtggcaa aaaacacccg ttcataatac gcgctgataa tgatcaggag tcacaccatg
    10141 accattacgt ctctggatgg ctatcgctgg ctgaagaacg atattattcg cggtaatttt
    10201 caaccggatg aaaaattacg gatgagttta ctgacatcgc gttatgcact tggcgttggg
    10261 ccgttgcagg aagcactttc tcaactggtg gcggaacggc tggtcacggt ggtgaatcaa
    10321 aaagggtatc gggtggcgtc tatgtcagag caggagctgc tcgatatttt cgacgcccgc
    10381 gccaatatgg aagcgatgct ggtcagtctg gcgattgccc gcggtggcga tgaatgggag
    10441 gcagacgttc tcgcaaaagc gcatctgctc agtaagctgg aagcctgtga cgccagcgag
    10501 aaaatgcttg atgagtggga tctgcgtcat caggcgtttc atacagcaat tgtggcgggc
    10561 tgtggttctc actatttgct gcaaatgcgt gaacggttgt ttgatctggc ggcgcgttat
    10621 cgatttatct ggctgcggcg aacggtgctt tcggtggaaa tgctggagga taaacacgat
    10681 cagcaccaga ccctgactgc ggcggtactg ccgcgagata cagcgcgcgc cagtgagtta
    10741 atgcgccagc atttactgac gccaattccc attatccagc aggcgatggc tggcaattaa
    10801 ctactcttcc ggaatacgca acacttgccc cggataaatt ttatccgggc tttttagcat
    10861 cggtttattc gcttcgaaga ttttattgta cagattagcg ttaccgtaga cctgtttgga
    10921 aatggcactc agagtgtcgc cagacttaac ggtataaaac tggctggcag tggctggtgt
    10981 cgccgttttc acctgatcat caacactggc aataccggaa atattcccca ccgcaacaag
    11041 gattttctcc ttcgcctcct gactcaggcc gtcaccagtg accgtcgctt tgccgtcggc
    11101 aatttgaatg ttcactttat cggcatcagg tataccggtt ttgctcagat gctcctgcac
    11161 cttcttcgcc tgatcgtctt tatcgtgctg acctgtaacc gcatcccaga gtttttctcc
    11221 ggcatcttta acaaaattga acagacccat agctacctca ttcgttaacg gaaacactca
    11281 gaaagtgtag cagaggaacg cggcgcgtta acgccgcggg ggtgagcaca ggtttaatcg
    11341 cgggtttgca cccagaacgc gtgaatcaaa ccggggatat acccaagcag tgtcagcaga
    11401 atattaataa tgaacgccca accgaaccct ttaccgagca gcacgccgat cggcggcaga
    11461 atgatggtga tgacgattct ccagaaaccc atatgtactc cctataagaa aattactcat
    11521 tgtttaaaaa gagattttat ctcttaagcg tagtaatttt agcggagcct gccagtttca
//
LOCUS       DAJTRD010000114        11110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTRD010000114 DAJTRD010000000
VERSION     DAJTRD010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.114"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO315_003438"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO315_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5892221.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="OO315_003439"
     tRNA            complement(621..695)
                     /locus_tag="OO315_003439"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="OO315_003440"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="OO315_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="OO315_003441"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="OO315_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5892222.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="OO315_003442"
     CDS             complement(2932..3990)
                     /locus_tag="OO315_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892223.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO315_003443"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO315_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5892224.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="OO315_003444"
     CDS             6068..6352
                     /locus_tag="OO315_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5892225.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="OO315_003445"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="OO315_003445"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5892226.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="OO315_003446"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="OO315_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5892227.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="OO315_003447"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="OO315_003447"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5892228.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2775 a         2543 c         2748 g         3044 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagccttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacttggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtccgttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtgg aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
    11101 ttaaggatgt
//
LOCUS       DAJTRD010000115        11046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTRD010000115 DAJTRD010000000
VERSION     DAJTRD010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.115"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            409..1239
                     /locus_tag="OO315_003448"
     CDS             409..1239
                     /locus_tag="OO315_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5892229.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMPYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO315_003449"
     CDS             complement(1380..2153)
                     /gene="uxuR"
                     /locus_tag="OO315_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5892230.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2368..3828)
                     /locus_tag="OO315_003450"
     CDS             complement(2368..3828)
                     /locus_tag="OO315_003450"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5892231.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO315_003451"
     CDS             complement(3909..5093)
                     /gene="uxuA"
                     /locus_tag="OO315_003451"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5892232.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5433..6776
                     /gene="gntP"
                     /locus_tag="OO315_003452"
     CDS             5433..6776
                     /gene="gntP"
                     /locus_tag="OO315_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5892233.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO315_003453"
     CDS             complement(7027..7929)
                     /gene="fimH"
                     /locus_tag="OO315_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5892234.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO315_003454"
     CDS             complement(7949..8452)
                     /gene="fimG"
                     /locus_tag="OO315_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5892235.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO315_003455"
     CDS             complement(8465..8995)
                     /gene="fimF"
                     /locus_tag="OO315_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5892236.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(9005..>11046)
                     /gene="fimD"
                     /locus_tag="OO315_003456"
     CDS             complement(9005..>11046)
                     /gene="fimD"
                     /locus_tag="OO315_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5892237.1"
                     /translation="IGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNKW
                     QHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPVI
                     HGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIFT
                     VPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQLA
                     DRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNIQ
                     LVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLTV
                     TQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGRD
                     QMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLSY
                     SVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHANG
                     VTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNTL
                     ADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQSS
                     GIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2684 a         2829 c         2682 g         2851 t
ORIGIN      
        1 acgtagatga aaatacttgt caccgctgct acttaattcc ggtgggaaat tatttatttc
       61 cgcataatca gctatgtatt atttgaaaca attagtttgt aaataatata aattgataac
      121 atatcaaata catcaaaaat accattcaca ggcattatta atatagacct caatatagca
      181 atatcaaatt ccaattagta ataagatatg cttttaaatt gcatgcaaat aattttaaat
      241 gagcgttttt tgatacttta gagtgtttcc taccctttaa tatcactgtg tagagttgat
      301 atcactacaa cagaaacatg catgtcattt gtttacttct ctttttacta cagaaaaatt
      361 tatctatttt tctaaacatc agttaaatac tcataaaagg taactattat gtctatgcca
      421 ttaagcaatg ccgctaataa ccaattcaat acaaataatc acttccttca tcatccaaaa
      481 gtcgacagtg agttaacacg tcgatatgaa tatgcacgcc tggatacgga aaatatctat
      541 cttctgcctc ttgctcgtgg caataaccat aattacgatg gaaaaagcgt tgtcgaaatt
      601 agaaaactta atattggcaa acagtcctgg ccatttaatt atgtgacaga tacatgcaga
      661 gaattcaatg gtattacaac aacaggacgt atgccctatc gaaacttgaa aatcacctca
      721 gctttagacg aaatatatgg tggtatatgt aaaaaggcgc atgctgctac agagttagca
      781 gaaggtttac ggctaaattt atttatgaag agccctttcg atcctgttga ggactataca
      841 gttcatgaaa taacattagg ccctggttgc aatgttcctg gttatgccgg aaccacgata
      901 ggatatattt caacccttcc agcttctcag gcaaaaagat ggaccaatga gcagccaaga
      961 attgatatct atattgatca gataataact gtctcagggg tagcaaattc ttccggattc
     1021 gcccttgccg cattacttaa tgctaatatt gagatgggaa atgacccaat cattggtata
     1081 gaagcctatc ctggtaccgc ggagatccat tcaaaaatgg gatataacgt tatccccggt
     1141 gatgaagatg cgccactcaa acgcatgact ctgcaaccgt catctcttcc agaactgttt
     1201 gaattaaaaa atggtgaatg gaattatatt ggtaaatagc cactgagaag aatgagtact
     1261 aatattcagg ttatacagat atttaaacaa aaccctgcaa tttacaaagt aatactcatt
     1321 gcgacttctt aatttaagaa agcgcaagga acgtttaccc ttgcgcttat tataataagt
     1381 caggcgtcga ctttatccag cggccatgaa tcaaagagat agccgtcaaa ataaatatcg
     1441 tcaacgttcg agaattccag cagacgttgc ttaacgtttt ccagatgctg ccacattgcc
     1501 agcttcgccg ctcgggcatc ttttttgatt aacgcggcga ggatctgttt gtgatcgccc
     1561 aaccactctt tgcgatacag gctgtcatcc agatggctgt gcaactggat ccacattgga
     1621 ttgttttccc gccactgcca ggactgacgg aataactcca ccagcatgct gttatgcgtt
     1681 gcttcagcaa tggcgagatg gaactgcatg tcaccgcttt cgctgctacc cggcgcgctg
     1741 gaagccagtt cacgctcttc cagttgcaat gcctgacgca ttttgacgat atcttcgcgg
     1801 gtagcctgca gtgcggcaaa ctcggcgatg ttactttcca gtaactgacg cgcctgtaac
     1861 agctcaaaag gacccgcatc attgcagacg ttggcatccg gactgtctgt gttgtggctg
     1921 cctgagctat caagcacata gataccggca ccccggcgca cttccaccag ccctttgatc
     1981 tccagcatga tcagcgcttc acgtaccacc gtccgcgtga catcaagcat ttctgcaatt
     2041 tcacgctccg gcggcagccg ttcgccagga ttgtacggcg tctttatgat cagatcgtgg
     2101 atcatcgccc cgacttcctg gtaaggtctt tgcgcagagg tgacagattt catggtgttg
     2161 tgtccgttag gtgcggttaa tcaaattttt gccggatgcg gcgtgaacgc cttatccggc
     2221 ctacaacggg caattttacg gcccccgtta atctcaaact ataccattag cactggaagt
     2281 tggcacgtac aggtgggcaa aaaaacgaaa actggtcaac caaatatcag tttgattgac
     2341 cagtaagtct gtagggtaat cagttagtta cagcgcagcc acacactcgc gcgcaccgcg
     2401 ttcgcacagc tgctgatatg ccgacgtcac cgcgccaaca aagtcggcat tctgcggcag
     2461 atcatcggca aaaataccgc tcaggcccag cagcgctttc acgcggtctg cgccctgata
     2521 ctgcgcgtta atcttctgga actcggccag catcgggtcg accacgtcaa tggcattacc
     2581 ctgctcatcc acgccctggg tgtaacccat ccagccagcc acgcccagcg ccaggtgacg
     2641 ccagctgccg ccgttttgca ggtgcagacg caccgggtcc agtagacgct gcggtaactt
     2701 ctggctgccg tccatcgcaa tctgccaggt acggtgacgc agagacgggt tgctgaaacg
     2761 ctcgatcagc agcgtcgcat aggcgttcag gtccgtacct tccggcatcg acagcgttgg
     2821 cgcttgttcc tgcatcatca gggcaaaggc cgctttgcga taatccgggt tagtcatggt
     2881 gtcggcaatg gtttcatagc cgccgaggta accgaggtac gccaggaaag agtggctgcc
     2941 gttcagcata cgcagcttca tcatttcgaa cggcacaacg tctgcaacga actgtgtgcc
     3001 cactttatcc cagtccgggc gaccattaac gaagttatct tcaatcaccc actgacggaa
     3061 cggttcgcag gcaatggcgc acgggtcgta aacacccagc tggtcagcaa tttcctgtaa
     3121 ggtttctggc gtcgccgccg gaacgatgcg gtcaaccatg gtgcacggga aggtgacgtt
     3181 ctcttcaatc catgccgcta gctgcgggtc acgcgcctga gccagcccca gtaccgcgac
     3241 cttcgccaca tggccgtttt cacgcacgtt atcgcaggac atcaccgtaa atgctttcag
     3301 ccctttttca cgacgcagac gcaaggcttc gacgatgtaa ccaatcgcgg acttcggcgc
     3361 agtcgggttt tccagatcgt gcttgatcag cgggttattg agatccagtt gaccgcttgc
     3421 cgcatcagcg cagtagcctt tttccgtgac cgttagagag acaatcgccg tttgcggacg
     3481 cgccatcgcg ttgagaatac cttcgcagcc gtcgatttcc ggatgcagcg cttctttcat
     3541 cgaaccgata attttcagct cggtgctttc tgcgcctttt tccgctacgg tgtacagcag
     3601 ttgctgtttt ttcaggtttt cgatcagcat gcggtcgttg cctggcatca ggttaacttc
     3661 gcagatgccc cagtcgctgt cggtgctttc cagcagatga tgggtataca gcgcctggtg
     3721 cgcgcggtga aacgccccgc aaccgagatg cacaatgcgt gattccagac gagaatgatc
     3781 ccatgacggg cgggcaaccg gcagattgct gtcaacaata gtagtcatcg taaactccag
     3841 aatccgtctt cagagagtga cggcaaggaa gaaggaaccg gcacggcgcg cagccatgcc
     3901 ggtgggtatt aacggctaaa gaaagcgcgc tggatcgcca gttcgacacc gcgaacttcg
     3961 gccaggcctt tcagacgacc aattgcggag taaccagggt tggttttctt cttcaggtcg
     4021 tccagcatct gatgaccgtg gtccggacgc atcgggatca ggtcttcttt gccttccgct
     4081 ttacgacggt gttcttcttc aacaatcgct ttcaccactt tgtacatatc aacgtcaccg
     4141 ttcaggtgcg ccgcttcgtg gaaggttttc gggttatctt cacgcatggt ggagcgcaga
     4201 tgggtgaagt aaatacgcgg accgaactgc ttgatcatat caaccagatc gttgtcagca
     4261 cgcacgccgt aggaaccggt gcacatggtg aaaccgttcg ccatgctgtt tacggtatca
     4321 accatccact gcatatcttc aatggtggaa acaatgcgcg gcaagccgag gatcgggcgc
     4381 ggcggatcgt ccgggtgaac cgccatacgc acgccaactt cttcagcaac tggaataatc
     4441 gctttcagga agacggcaaa gttttcgcgc agtttggctt tgtcgatatc tttgtacagc
     4501 tccaggtgct tacggaactg gtcgagggta tacccttctt ccgcgcccgg cagaccggca
     4561 atgatgttac gcgtcagacg cgctttgtct tcgtcgctca tagtggcgaa gcgttcagct
     4621 gcctgagcaa tttcttcttc ggtgtaatcc gcttccgcgc ctggacgttt caggatatgc
     4681 atttcgaatg cagcgaattc gatctggtcg aagcgcagag ctttggagcc gtctggcagc
     4741 acgtattcga ggtcagtacg ggtccagtcg agcaccggca tgaagttgta gcacacggtg
     4801 cgaatgccac actgcgccag gttgcgcaga gtctgctgat agttagcgat ccactgctca
     4861 tagttgccag tgtgggtttt gatatcttcg tgaattggta cgctttctac gacagaccac
     4921 accaggcctg cgtcttcaac gatcgccttg cgtttgagga tctcttctac ggaccatact
     4981 tcgccgttcg ggatatggtg cagcgcggta accacgccag ttgcgcccgc ctgacggaca
     5041 tcagctaaag aaaccggatc gtttgggccg taccagcgcc aggtctgttc catgattttt
     5101 cctctttcac atagtgggtt ccacagcaaa gagacgcgga accttggagt tgtttaggta
     5161 tccggatgaa caaccagacc gcagccgtgg cgttgcatcg gatgagatgg cgtataagtt
     5221 ctaccttttg gtaagacatg tcaaccagaa ttctgaaatt ggttaaccgc gtcgcaagaa
     5281 tttcactctc atggttgttg ctgcaaattg cagcaaaggg taatcgatgg ttattgatca
     5341 gaggatgtga cattcatcgc aacaatggtt gaccaattta cataacatgt cgcccaaaat
     5401 aacaacggtt caaccaagta aggtaaaacg ttatgcatgt gcttaacatt ctctgggtgg
     5461 tattcggcat tggcctgatg ctgatactga atttgaagtt caaaatcaat tcaatggtgg
     5521 ctttgttggt ggcggcgctg tccgtcggga tgctggcagg catggatttg atgtcgctgc
     5581 tgcacaccat gaaagcgggc ttcggcaaca cgctgggaga actggctatc atcgtggtgt
     5641 tcggtgcggt catcggtaaa ttgatggtcg actccggcgc ggctcaccag atagcacata
     5701 cgctgctggc gcgtctcggt ctgcgctatg tacagctgtc ggtgattatc atcggcctta
     5761 ttttcggtct ggcgatgttc tatgaagtgg cctttatcat gttagcgccg ctggttattg
     5821 ttattgccgc cgaagctaaa attccgttcc tgaaactggc gatcccggca gtagcagctg
     5881 ccactaccgc acattcactg ttcccaccgc agccgggtcc ggtggcgctg gtgaatgctt
     5941 atggcgcgga tatggggatg gtttatatct atggcgtact ggtgacgatc ccaagtgtaa
     6001 tctgcgcagg tctgatcctg ccgaagttcc tcggcaatct tgagcgccca acgccatcat
     6061 tcctgaaagc agatcaaccg gtagatatga ataatctgcc ctctttcggc gtttcgattc
     6121 tggtgccgct gatcccggcg atcattatga tctctaccac catcgccaat atctggctgg
     6181 taaaagatac ccctgcctgg gaagtggtta actttattgg ttcctcgccg attgcgatgt
     6241 ttattgcgat ggtggttgca ttcgtactct ttggcaccgc gcgtggtcat gacatgcagt
     6301 gggtgatgaa cgcttttgaa agcgcggtga agagtattgc aatggtgatt ctgatcatcg
     6361 gtgcgggtgg cgtgctgaag cagaccatca tcgacaccgg cattggcgac accatcggca
     6421 tgttgatgtc ccacggcaat atctcgccct acattatggc atggctgatc actgtgctaa
     6481 ttcgtctggc gacgggtcag ggtgtcgttt cggcgatgac cgccgccggg attatcagtg
     6541 ctgcaatcct tgatccagca accggtcagc tggttggcgt gaatccggcg ctgctggtac
     6601 tggcgacggc tgcgggttcc aacaccctca cccacattaa tgatgcatct ttctggttgt
     6661 tcaaaggtta ctttgacctg tcagtaaaag acacgttgaa aacctggggt ctgctggagc
     6721 tggtcaactc cgtggttggg ctgattattg tgctgattat tagcatggta gcgtaaaaga
     6781 acaccagagc ctgccaatgg caggctcaga ctgatgacta aagtctaaaa atgtgcccgg
     6841 acagtcccct cgcccctccg gggagagggt tagggtgagg ggaacagacc agtaaagtgc
     6901 gctttgttta tgccagatgc gacgctgacg cgtcttatct ggcctacaaa gggctaacgt
     6961 gcaggttttg taggtctgat agcgtagcgc ctcaggtacc agcattagca atgcgctgta
     7021 atttttttat tgataaacaa aagtcacgcc aataatcgat tgcacattcc ctgcagtcac
     7081 ctgccctccg gtacgtgcgt aatttgccgt taatcccaga cttaccgccg aagttcctac
     7141 tgctcctaac gataccgtgt tattcgctgg aataatcgta ccgttgcgcg tcaactgtac
     7201 gccgacgccc tgcgctggtg aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc
     7261 tgcggttgtg ccggagaggt aataccccag gttttggctt ttcgcacaat aaacggtaag
     7321 aggaattggc actgaaccag ggtagtccgg cagagtaacg gtgacatcac gagcagaaac
     7381 atcacaaccg ccagtgggca ccaccacatc attattggcg taaatattcc acacaaactg
     7441 aaaatcatcg ctgttatagt tgttggtctg tcgcaaaata agcacggcaa ttaatgaacc
     7501 agctttaata gccaccccgc ccgcactgct cacaggcgtc aaataaagcg ccaccggcca
     7561 cggcttatcc gttctcgaat tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg
     7621 atagctactg ccactatatt ttacggtccc ggaaaaatta gataacacgc cgccgtaagc
     7681 cgagcctcgt tgcagtgtga catagtctgt aatggtttcc ggataatcgt tatggcaaaa
     7741 gatttgcgtc gaaagatcta cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt
     7801 tacataaaca ttagcgctgc caccgccaat agggatagcg gtaccattgg cggttttaca
     7861 ggcgaatgac caggcattta ccgaccagcc catcagcagt acagcaaaca gggtaatagc
     7921 tcgtttcatt acaattatct cttcgggttc agctgtaggt ataggtgatg ctaatcactg
     7981 cctgaatggt tccctgagtg gctccgccat ttaccgtcaa tgctctgacc tgtaacggga
     8041 agtgcgctga ttgtgaggaa tcatccacct gaactgtttt ggttgcgcca gtattcaatg
     8101 tgttgccact gtcatcctgt agctctaact ggatgttttg cgcggtcccc tggtttttat
     8161 aatatccggt gctgtcggct gccccgctga agctggcagt gaccctcgac gttcccaccg
     8221 gacaattagt caactcaagc gcaacatcat gccaggccga tgccgccccg gcagacataa
     8281 gactgaaaga ataaagatcg ccgagatcta ccgtggcatt ggtggtggaa accgtacacg
     8341 gtttggcgac gaccttaccg ttcaccgtga tggtgacatc ggctgcctgt atcgtcgcac
     8401 ttgcgagcgc caacattgcc gccaatacat acccacgttt gcaccatttc atgagcatct
     8461 ccagttactg atattcaaga gtgaaggtag ccgtagcatt gatatgcccc gcagtgacag
     8521 gcacctgtgt cgccattagc cgggcgtaaa aattcagcgt atttggttta cccggcgtca
     8581 gggtcgtcca cgaaatcgca gacgatggag cattaagggg tatttgattt tgctgctcat
     8641 tcagaagctg tattcccagt cccgcagccg ctgacaccgt attttcaagt gcaagcaggt
     8701 tggcattgtg gctatctgca acgccggtaa acccaacttt tacggcagaa acggcattac
     8761 cacagggtga cagcaaaata cgaaatggaa cgacaggagt cgtcgcgcca atgttgttaa
     8821 attgcttcgc cgcgttttcc atcagatcaa cagtaaaatt ggttgattca gcggccacac
     8881 tacagccgtt atctctgaca tagccgcgga tagtaatcgt gctatccgca gccaaaacgc
     8941 gacttaccgc cagccacaaa aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc
     9001 ccccttaacg acattcagct gatagctggg ttaataactg ctgctgactc tctggtggca
     9061 gttgataatt ggcgacacag tgagcatttt cctcttctcc ccatttcacc tgaacttttc
     9121 ccgctaaagg cattccgctg aggtaaacct gaccattatc cgcaacaatg ccgctactct
     9181 ggctactctc tgatgtcacc atcgccccaa acggcagcgg cttattattg tgagttagcg
     9241 tcatgagcag ttttatccca acgcgcgctt taaactctgc tcgcacgatc gccccacgag
     9301 tgggaacaac gttagcgacc gcgttatcta aatcgacgtt atcagccaag gtattggtat
     9361 ccagcgccac tctattttcc cgatattcag tggcataagg cagcacggca taaccacgcc
     9421 agtcggtacg cacccccgtc tggttttcga cttttgcatc ttttgcgcca ggcgctttaa
     9481 caagcaccac cgtatcgttt aacggctgcc ccagcgttac gccattggca tgagccaata
     9541 ccccaccgct gactccgtaa tagagctgct taatatcatc gctatggctg taaccgatat
     9601 tggcattgcc gtaaccaccg cgataattca gcgtggcgta gcctgtactt ccgctattac
     9661 catcgcctcc cccggcatag ccggtttgca cgctataact gaggttgttg tcttccagca
     9721 acgtaccgta tacaccagcc agattggtca tccgaccgtt gagatcgtgt gacatgctgt
     9781 agctggcact ggcatgtcgc cactgagatt tactgtcaga acgcagccag tggctgaaag
     9841 gaatattgac gttaagcgct aacatctgat cccgtccttt ttgccaggcg tttttcgtca
     9901 ggctatagct gagcgtccag ttgatatctt cgaacgcagt atttaatcca gcctggaatt
     9961 gctcatcgac attactcgtt ccccaataag tttgatggct accactcaaa tacagtgttg
    10021 atgtgcgccc gagttgctga gtaacggtga gctgtaattt cccgcgtttg ttataagcga
    10081 ggttgtaata gtcggtgaat ttcggcttaa cctgaataac tccatcctgt gtttcgatgt
    10141 tgtagccatt cattcgactg tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat
    10201 aacggtaacc cactaactga atattcgtgc ctgattcatt gagcgatttg ttatagagaa
    10261 aacgcaccga ttgtccgtca tgctgactgt catcgggaag tgtggaatta gcctgcgtca
    10321 tatccacaga cagagcgccc agtgcctcca tgtttttccc gataccgaaa ttaaaagcac
    10381 gataacgatc cgccagttgc gttccaccat atattgtcca gccagccgga aggccgtgga
    10441 gtaatgtact ctggaaaaag cggggttttt cctgctgcgc atttccacta cggtattctc
    10501 ctgccgtaat ggaataacga gtatgccctt cacgttgcaa aagcgggact gacgaatagg
    10561 gtacggtaaa aatctgcgtg ctgccgtcag cctctttgat cgttacctgc aagtcaccac
    10621 tattacctgc ggcatagata tcgttgatgg taaaaggccc cggtggcacc gtactattat
    10681 aaatgccata tccattttgt ttaatagtga cctgtgcagt accacgagca ataccgtgga
    10741 tcaccggggc aaatcctctt tggctatcgg gtaacatatt gtcatctgag gccaattgtg
    10801 cgccgcgaaa gttaatacca tcgaaaatat cgccctgggt ataaccatca cccaacgtca
    10861 gccgggaacg taaaggaatt atgtctcgct caatccaggt aatgatatgc tgccatttat
    10921 ttttgctacc tgatgaactg tcgctactgt tataactcca ggtggtattg tcgcgtaaac
    10981 gccacgcacc aatatttaac ccactctgta ggtttaaata tgcataatgg ctgttacccc
    11041 caatcc
//
LOCUS       DAJTRD010000116        10993 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTRD010000116 DAJTRD010000000
VERSION     DAJTRD010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10993
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.116"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(60..1274)
                     /gene="rspA"
                     /locus_tag="OO315_003457"
     CDS             complement(60..1274)
                     /gene="rspA"
                     /locus_tag="OO315_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5892238.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1480..1806)
                     /locus_tag="OO315_003458"
     CDS             complement(1480..1806)
                     /locus_tag="OO315_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5892239.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1941..2282
                     /locus_tag="OO315_003459"
     CDS             1941..2282
                     /locus_tag="OO315_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5892240.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2348..2877
                     /gene="speG"
                     /locus_tag="OO315_003460"
                     /pseudo
     CDS             2348..2877
                     /gene="speG"
                     /locus_tag="OO315_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2880..3590)
                     /locus_tag="OO315_003461"
     CDS             complement(2880..3590)
                     /locus_tag="OO315_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5892241.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3698..4003
                     /locus_tag="OO315_003462"
     CDS             3698..4003
                     /locus_tag="OO315_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5892242.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4202..4522
                     /locus_tag="OO315_003463"
     CDS             4202..4522
                     /locus_tag="OO315_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR5892243.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5082..5660
                     /locus_tag="OO315_003464"
                     /pseudo
     CDS             5082..5660
                     /locus_tag="OO315_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6346..7335)
                     /locus_tag="OO315_003465"
     CDS             complement(6346..7335)
                     /locus_tag="OO315_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892244.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLSTNADPQVYALQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7420..7911)
                     /locus_tag="OO315_003466"
     CDS             complement(7420..7911)
                     /locus_tag="OO315_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892245.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8232..8732)
                     /locus_tag="OO315_003467"
     CDS             complement(8232..8732)
                     /locus_tag="OO315_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892246.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8722..9237)
                     /locus_tag="OO315_003468"
     CDS             complement(8722..9237)
                     /locus_tag="OO315_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892247.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9668..10036)
                     /locus_tag="OO315_003469"
     CDS             complement(9668..10036)
                     /locus_tag="OO315_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892248.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10448..>10993)
                     /locus_tag="OO315_003470"
     CDS             complement(10448..>10993)
                     /locus_tag="OO315_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5892249.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
BASE COUNT         2823 a         2794 c         2714 g         2662 t
ORIGIN      
        1 ccatcttaaa tgactctgag gtgtctgtta aacccgtggc gattcaatta tcttactcct
       61 tacctgttcc acagcgtgcc atcttccaga cgtgcgactg gtagataagc aggttcatag
      121 ggatatttcg ccgccagctt ttcatcgaat tcgataccaa gacccggttt gtctcccgga
      181 tgcatatagc cgttatcgaa agtccagttg tgcgggaaga cttcgagcat ttgttcggaa
      241 taacccatgt attcctggac accgaaattg gggacccaca gatcaaagtg cagcgccgca
      301 gccatgcaga ctggtgacaa atcggaagga ccgtgtgagc cagtacgtac ctgatacagc
      361 gaagcaaaat cggcaatccg gcgcataccg gtaattccgc ctgcatgggt cagcgtggtg
      421 cggatataat cgatgagttg ctcttcaatc agttgtttgc agtcccagat gctgttgaag
      481 acttcaccca ctgcgatggg tgtgacggta tgttggcgaa tgagacggaa gcattcttgg
      541 ttttccgctg gcgtcgggtc ttccatccag aacatgcgat aatcttcaat gcttttacca
      601 aagcgcgccg cttcaatagg cgttaagcga tggtgcatgt catgcagcaa atgttcatta
      661 aaaccaaact tgttacgtac cgcgtcaaac aatttcggca tgaaatcgag gtatttctcc
      721 gtcgaccaca gctgctcttc cggccactgt cctttggttg cgggttcata agccagacct
      781 ttacctttcg acatgccgta ggtggttttc ataccaggga ttccgcactg cacgcggatg
      841 gctttgaatc ccagctcctg atgacgggca taatcatcca gagcttcatc aatactgtga
      901 ccggtggtat ggcaataaac catcacccct tcacgagacg cgccgccgag taactggtaa
      961 agcggcatgt tggcagcttt ggctttaata tcccacagcg ccatatcaac cgctgaaatg
     1021 gccgacatcg taaccggacc gcgacgccag taagcacctt tatagaaaaa ctgccagata
     1081 tcttcgatac ggtgcgcatc gcgaccaata agccgcggac aaagatgatc ctgcaaataa
     1141 gaggccacgg aaagctcacg tccattgagg gtggcatccc caaggcccgt aataccgtcc
     1201 tcagtggtga tttttaatgt gacgaaatta cgccccggac aggtaacaaa aacttcagcc
     1261 tttacgatct tcatgttcga ttccttgcat cgcttgtcgt gatgcatgaa atctacgcaa
     1321 ctgagctact gccatacaag tatgaagatc gaaaaaagcc ggagtgatca caaaaaaagg
     1381 cgtatatttg cgctgtgaat ggttgacaaa agatgaaata gaataccttt tgtcagctga
     1441 cacttcctcg tatcttattg ataaaatgga tttatgttcc tacgtgcgcc cccagcccgc
     1501 aacaatgatc aacattccgc aaagcgcaat caacgcaccc gtccagtcat aaagacttag
     1561 tttcacgcca tccacaacgc gcagccacat caatgccgtg cagacataaa cgccaccata
     1621 agccgcgtaa acacgcccac tcgccgcagg atgcaacgtt aacaaccaga caaacagcgc
     1681 cagtgaaatc cccgccggaa gcaacagcca gatactggcg tttcgtttta accacaacca
     1741 gggcagaaag catccaataa tttcacacag cgcagtagca aaaaatagta acgttgtttt
     1801 aatcatcttt gtcacttatt gacatcatgt atagttatag ggcgacataa tatcatcaat
     1861 ataaacatcc tcctggtacg ttttgcgttc gcagtggatg gtgtagaatc acctttaatc
     1921 attcatacag ggaatgaatt atgaaaatca ctctcagcaa acgaatcggc ctgctcgctt
     1981 ttctgctgcc ttgcgcactg gcattgagca caactgttca tgccgaaact aacaaactgg
     2041 tgattgagtc tggcgacagt gcacaaagcc gccagcacgc cgctatggaa aaagagcaat
     2101 ggaatgacac gcgcaatctg cgccagaaag tgaataaacg cactgaaaaa gagtgggata
     2161 aagccgacgc cgcttttgat aaccgcgata aatgtgagca aagcgccaac atcaatgcct
     2221 actgggagcc caatactttg cgctgcctgg accgtcgaac tggccgcgtt attaccccct
     2281 aacctgttat tgatttaagg aatgtaagga cacgttatgc caagcgccca cagttaagct
     2341 acgcccgctg gagcgtgaag atttacgcta tgtacatcaa ctcgacaata acgccagtgt
     2401 gatgcgttac tggtttgagg aaccctacga agcctttgtt gaactctctg atctgtatga
     2461 taagcgcata ttcacgatca gagcgaacgg cgctttgtgg tggaatgtga cggcgaaaaa
     2521 gccggtctgg tggagctggt ggaaattaac catgttcatc gccgggcaga atttcagata
     2581 attatctccc cggagtatca ggggaaaggt ctggcaaccc gtgccgccaa attagcaatg
     2641 gactatggct tcaccgttct caatctctat aagctgtatc tgatcgtcga taaagagaat
     2701 gaaaaagcga ttcacattta ccgcaagctt ggctttacgg ttgaaggtga attgatgcat
     2761 gagtttttta ttaatggtca atatcgtaat gccattcgca tgtgtatatt ccagcatcag
     2821 tatctggcag agcacaaaac accgggtcag actctcctga agccgaccgc acaatagcat
     2881 taataataat cgatcgtatt tttgatggtg taaacccgtt cgacggcggg ttttactcct
     2941 tcatcaacaa tgatcagctg acagtccact ggattggcgt gactgtcata ttcacagctc
     3001 tgttttacat taccaactcg ttgattattt agtaaagtaa ccgctgtgta atctaatttt
     3061 ttgatcggat ccgttgatgg cgtggcgctg acagataatg ttttgtcgtt acttttcgtg
     3121 gttttaccca gcggataccc ctgatcatca tagcgatatt ccatctgcat ttgtttgctg
     3181 ctggctttaa tcacgaagcc attatcatcg gtttcccaac tcaccccggc agaaggtaat
     3241 tctgctagct ggcattttcc ctgtaaacgt actctcttct ccagcgtctc ggcatcacgg
     3301 taataattgg cgtccagtac cagagcgacc acggtattat tttccagatc cagtaattcg
     3361 agtgaatcaa aacagccttc ttccgacaaa gtcccagaaa cacgtttcgt cacttcacct
     3421 tgctcatcca ttaatgtctg agtgaaatct tttaccggac cacgcagcgg atcaaaatcg
     3481 aattcattag agaaactggc catctcaggg gtaaatgaaa gtgttacctc tgtgcggtca
     3541 catcctgtga ggaatatcgc gagtaagcat ggtagtaatt tgtatttcac aacagtcacc
     3601 agagagtaga gatgattctc aatcatagta gcaaatacag tactttacac gttaaatgct
     3661 atgcttaaag aagttatctt cgcgtaagga gcttatgatg aaactctcaa cctgctgtgc
     3721 cgcgcttctg ctcgccctcg cctcacccgc ggtactcgcc gcgccgggat cttgtgagcg
     3781 catacagagc gatatatcac agcgcattat caataatggc gtacccgaaa gcagcttcac
     3841 gttaagtatt gtacccaatg accaggttga tcagcctgat tcccaggtcg tcgggcattg
     3901 cgctaatgat acgcataaaa ttctctacaa ccgcaccacc agcggtaacg tctctgctcc
     3961 cgcgcagtct acccaggatg gcgcgcctgc cgaaccgcag taacacatta tcgactgaac
     4021 gccggatatg acacatccgg cgatttgaac atacaacata atctcacctt attactcata
     4081 cccttctatt gatgtggatt aataattctt aacccaaaat gggtagactc cctctattgt
     4141 tagcgcgcta aatattcaat gtataaacat ttatataaca ataaagaaca gggagtgagt
     4201 tatgtccaaa aatgaccgaa tggtgggcat cagccgcaga acactcgtta aatcaaccgc
     4261 gacaggttct ctggcgctgg ctgcaggcgg tttttcttta ccgttcaccc tgcgcagtgc
     4321 agcggcagcg gtacaacagg ccagcgaaaa agtgatctgg ggttcctgtt ccgtcaactg
     4381 tggtagccgc tgtgcacttc gtctacatgt taaagataat gaagtgacct gggtggaaac
     4441 tgacaatacc ggcagcgatg agtacggcaa ccatcagggt acgcgcctgt ttgcgcggtc
     4501 gctccatccg ccggcgtatt aatcatcccg atcgcttgaa tgacccaatg aaacgcgtgg
     4561 gtaaacgcgg tgaaggcaaa ttcgaacgga ttagctggga tgaagccctg gatactatcg
     4621 ccagtagcct gaaaaaaacc gtcgaacaat atggcaatga agctgtatat attcagtact
     4681 cttcggggat tgttggcggc aatatgaccc gctcttcgcc atcagcctcg gcggtcaaac
     4741 gcctgatgaa ctgctacggc ggttcactca atcagtatgg ctcctacagc accgcgcaaa
     4801 tttcctgtgc catgccctac acctacggca gtaatgatgg caacagcacc acggatattg
     4861 aaaacagcaa gctggtcgtg atgtttggta acaacccggc agaaacccgc atgagcggtg
     4921 gtggcatcac ttatcttctt gaaaaagcgc gcgagaaatc gaacgccaaa atgattgtta
     4981 tcgatccgcg atataccgat acggctgccg gtcgtgaaga ggaatggctc cctattcgcc
     5041 cgggcaccga tgccgcgctg gttgcgggta ttgcctgggt attgattaac gaaaatctcg
     5101 ttgatcaacc ttttctcgat aaatactgcg tcggctatga cgaaaaaacc ttacccgcag
     5161 atgcacccaa aaatggtcac tataaagcct atattcttgg tgaaggtgac gataaaacag
     5221 caaaaacgcc gcagtgggct tcgcaaatta ccggtatccc ggtagaccgt atcatcaaac
     5281 tggcgcgtga aattggcaca acaaaacccg cttatatctg ctagggctgg gggccgcaac
     5341 gccaggcaaa cggcgagctg actgcacgcg ctattgctat gttacctatt ttgacgggca
     5401 atgtcggcat cagcggcgga aatagtggcg cgcgtgaatc gacctatacc attacgatag
     5461 aacgcctacc ggtgctggat aatccggtca aaacgtcaat ctcctgcttc agctggacgg
     5521 atgctatcga tcatggcccg caaatgacgg cactccgcga cggtgtccgg ggcaaagata
     5581 aactggatgt gcccattaag ttcatctgga actacaagga cttgaagcgg acccttagta
     5641 ggcctgaggt cctgcattga gtcaagacct tgtgtcattc tttgtgccag acttgtgtca
     5701 ttagacggga tgtctagacg gctggtcagg accctccggt ggcctcggtc tggacttgag
     5761 gcgggctatg ggggaagtct gctggcttaa tgtattagat ggtcattcac atttttctgt
     5821 cagaaaccat cggtcccact gtgagtacaa acctaagccc ccagaagccc acccacagcc
     5881 ttagacagag tgtcgaatac attcacaccg agtcgaccgg agaggaccat atagaggccc
     5941 acagagagga acacgagttt atagaccaga gtcaggaggg accttaggtc ttcggaagtc
     6001 atggacataa agcccccccc tatcttctat tgcactaccg cactagacaa ctagtacaaa
     6061 acaaaggccc agaccattgg tccagaccgt agttagttaa tcacagtgac aagccaccac
     6121 ctgaagtcct gctgatattc agtccagact tacctctgct catggtctta cctccctcgg
     6181 tctcctgacc ttgggtatcg actacagctt gctgagcgtc atctggagga gctacaggag
     6241 gagctacagg aggcgggacc gaagccccac cttgagtccc accaccacct gtcaggccct
     6301 tggcgatgga cctgaagcct ttactaatgc tcttacccat tggtcttacg cttcggtctt
     6361 ggtgaacttc aggcgtttga ctacaggcag gacggcagta gctgagccag tgcccatctt
     6421 gatggtcgta gcagcttcag cgacaacgaa cactttaccg aggatttcca agtcgtggga
     6481 ctgagagtcg ccatcacgtg aaaggacgac cgtaatattc tcaccgtcgt gacggaagca
     6541 gccgaagctc agggccgtga agcgtttagc cagcatgaat acacccttgt cttcaccgtt
     6601 gtaataggcg atgtctgtcc ccagcatatc gacgatagcg tcttcaccgg agaagccttg
     6661 cttagcagcc agacgggaca gtgctttcag ttggctctca tgagcgataa taccgtagtc
     6721 agacacctca gaaccgaaag aagactctgt gttctcatac agagcgtcca tgatagcgtc
     6781 tgccttagcg gtcaggctac cagtcaggac aggaagctct acggtcttca gcagcttaga
     6841 ggattccaga acgtcgacca tgcgagcgtt gatggtacgg accacattct cacagatgtt
     6901 catagcgtcc tgctgaagtg tgccctgagt gaatccggtg gatgacttgg cgtcccagag
     6961 cttggtctgc tcagagatga gaatagcctg ctcgattggt tcgaccacga tttcttgtcc
     7021 agacggaacg gtcacgactt caggtcgctt gccaccacgg acaggaacga ccacagagcg
     7081 gtcagccaga gtgtgagcaa tgattggggt cggtaagctg ttacgggtcg gcaggacttc
     7141 tacagggaac gggatgttct cttgagcgtc tttaagtaca ccgttcagca tagccttaac
     7201 cgtcttagct aactggaggg catagacctg cgggtcagcg ttagtcgaca gaccttcatt
     7261 gacctgaatg ttgtcggtat cggtgatgcg tactggctca atgccaaact ggaaagcgtc
     7321 tttaatcgta gtcataataa tcaatctcct aaattagttt aaatactgtg agtcctcaaa
     7381 actgaggagt ccctaacatc gggtcaggga ctgtgtgttt tacaagctgt ccatccagtt
     7441 accgcctgac tcaaggtcag cgacaatagc ggcacgggag cgaccagcgg aatgggtagc
     7501 actgcggagg ctctcaccat aggtccggcg ttcagctttg acctgttcgc ggcgctgtag
     7561 ctctttctgc cactctgcat cggtcatgtt ctcttcacgc atacgctcag cctgaagtcg
     7621 ttgctcttta gcgatacgct cagcttcagc tcgtttgttc atctggcggc gaccataggc
     7681 ggtcatcaag tcattactga agaagttgcc aatggtcacg ccagcttcag ccagagcttc
     7741 cagtaactcc attgcctcgc ggctggtaga cggcaggcct gacaggtagc ccggaatgag
     7801 ggtcttgata gcgagcgaca gcggataggg ctgaccgtcc agactcacag tgttttcata
     7861 aaggttgatg tcggtgactg acagcttcca gttgttgttg ttgaacatca taggtaattc
     7921 tcctcttgag agttgagact atcggtatcg cagacgcagt agcgcctacg ggtctccccg
     7981 catcggtcat ctcaaaaggt ttaaaattag gtttggtatg gtcaggactg cttagcaaag
     8041 gctcggcgta actgattgtc taaatcacgg tccaccagca tagctatcag ctcgtctcga
     8101 ctgtagtaag ggcgctcagg gtcttcgact ccaagtctca gcagagacag cagatgacgt
     8161 gtgggcaggc cacggtaacg aagggtcgct ttgccgatag gtgtacgagg gtcaatcata
     8221 gccatgatta cttacctcca ctcacagcga ccagcggtca cagcttgata gtctgaatac
     8281 tcagcctgtg agacttcacg tccggcgatg tagaggcctg tgccatgacg ctggatgaat
     8341 tccttggtct ccggcttgat gtcctcagca gacgctacag cagactgcac ggtggcgctg
     8401 gaggtcatag acacgaaggc tcgggttact gccagtccgt acccaaggtc caaccaccga
     8461 ccgaactcta tagcggctac tgggtcagcc agccacgagg tccctaagtg acccatgtta
     8521 atctttttaa caaacccgtt ttctgggttg gttagacttc taccagcctt ggtcctcatg
     8581 aagcgagcaa cctcggtact acggttatct tctaccttac caatctgaat gcctacccgg
     8641 cgtatctcat ccagtcgaaa caatccgtcc tgactggtga tgtcctgacc gaagatatga
     8701 atggtgtatt cctgcttatt gtcagaagtc ataagaggca cctcccgcca gagcttcggc
     8761 aaaggacttg aagcgctgtt cttggtcagc tttcgcctga aggcgttcgg catcaggtgg
     8821 gaccagctta tcaaaggtgc cgttttcgta atcttcagtt aggacctgaa ggcggcggga
     8881 ccatttgctc tccacctgca tcatcgcgtt agagatgtgc ttcaagtcgg catcagagcg
     8941 tgacgctatc caatccatct cttctgagtc accctcacag gtgtaaggat tgttgaaata
     9001 gaagatgtcc tgcaggccac gcttcagctc ttcctcggtg tgtgtctgga ggtatgactg
     9061 gataggctcc gcgtacatct cccggatttt ctcatagtcc cagtcttccg cctgggcata
     9121 ccatcgggat aacttcagca ggtctaagtt atagcgacag caatcgacac tggactctgc
     9181 attggtcagc aggatgatta agaactggcg gttagttact ggtaattcgg tattcataca
     9241 aagatgctcc tttggtaaag cactttctgt ctccacatgg agtccgcacc attcagacgc
     9301 atcctaaacg gacccatcag aaagtgctta acgaaagtga atggttaaat gttgacggtc
     9361 tggagtgacc atgttctcca tgtacgtata aaggtgcgac cgcatgtcag gacgagctgt
     9421 aaggactcca gatggtgagc ggtgatagac ttaagccaat gtagtgagta gacgagctac
     9481 taaccgagac gactaaagaa cgctaacgag ctggaggtcg atacaggagg tctgtcagag
     9541 gcggtcacgg gccttgatta cttaggagag atacttaatg tcggttgacc agatgctcgc
     9601 atgttctgac gcatcagact cagaggcgga ggctggtctc cctatagtga tagtttaggg
     9661 tccgtaatca tgaaccagct ttactggcgt ggtactggac ggatttactg gcgctgacga
     9721 cggtagcata gacgtagcga gcgtagcgag ttgaaaccat aggttcaccc aaggcaacac
     9781 gcttacggct cagggactcc tgcacagcgc ttacagttaa tgtgtcgtgc gttagtagtt
     9841 gggtcagaat caagtatggg ctgacaggct tggtattgct tgccattgat tcgttcaggc
     9901 gaccagcacc agtaaggaca tcatccagcc aaccactcag gtcgccctga ccatcgaatg
     9961 acagcaggtc cccgcagtgc tgctgcaagt aggctttggt gaacgtgttg tcgtgagttg
    10021 cgattggtcg tgccatagag tatctcctgt agttgaatta gttggttccc ctatagtgat
    10081 aatcaagtcc atcactggtg atgcatacag tagcgcgaca ggatgtgact cacaggttga
    10141 accgaatgtc aaggactaaa gacgcctcag gaattaagga agtatatata aagagagacc
    10201 agaggcgact cacagttaaa caggaatgtc aagaggagac cataggaatt tggagggact
    10261 ggagtttagg acgcagttta aataagacca cagacagact tagagtcgga cagccgaagg
    10321 cggaccagtg ggcctatagt caggagggat tatgattatt atcaccctcc ctaacttcag
    10381 tcagacttca gtgacccaag gtctcaacca tcaagccgta tatggtctcc accttgggtc
    10441 tccctactta aagtaaagct gtcttaagca catcagccag cctacccaca gcgtgtcctt
    10501 tcccgtacag gtcatacgta attgactgac tgctgtgacc taagatgcct tgagcgtcca
    10561 ccagattgac tccacgctct ttcattcggg tcgccagcca atgtctcaga ctgtggagag
    10621 agacatcctg agagtctcct agggcctcag tcaggaccct tgagttaaac cagctagagt
    10681 aggcgctcgg agtcatcccg aagagaggac cctcatcagg ctgcatgtct acccacgaca
    10741 ggaaggacgt caggtcaaac ccataagcgc catcggttaa cggcacaagt cggactgaat
    10801 gtttgttctt cacagacttc ccgtccccat cttcattgat gtcgatacag accagtccat
    10861 tgtccaaggt tacaatgtca cgtttagtca ggtggcagac ctcagcagac cgagcgccag
    10921 tgaccacaga caccagagac gcccatctac gggcctcaga ggtcgtgtga gccgtgtgtc
    10981 gtgtgaactg ata
//
LOCUS       DAJTRD010000117        10953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTRD010000117 DAJTRD010000000
VERSION     DAJTRD010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.117"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..241
                     /locus_tag="OO315_003471"
     CDS             <1..241
                     /locus_tag="OO315_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892250.1"
                     /translation="NGSVRYCQYTSMFGASYRYSGAGVGYQVASVVGGGFTPFIAAAL
                     ITYFAGNWHSIAIYLLAGCLISAMTALLMKDNQRS"
     gene            343..1797
                     /gene="amn"
                     /locus_tag="OO315_003472"
     CDS             343..1797
                     /gene="amn"
                     /locus_tag="OO315_003472"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5892251.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2140..2856
                     /locus_tag="OO315_003473"
     CDS             2140..2856
                     /locus_tag="OO315_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5892252.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(3312..3387)
                     /locus_tag="OO315_003474"
     tRNA            complement(3312..3387)
                     /locus_tag="OO315_003474"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3352..3354),aa:Asn,seq:gtt)
     gene            complement(3488..5115)
                     /gene="yeeO"
                     /locus_tag="OO315_003475"
                     /pseudo
     CDS             complement(3488..5115)
                     /gene="yeeO"
                     /locus_tag="OO315_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
     gene            5120..5195
                     /locus_tag="OO315_003476"
     tRNA            5120..5195
                     /locus_tag="OO315_003476"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5153..5155,aa:Asn,seq:gtt)
     gene            complement(5233..6183)
                     /gene="cbl"
                     /locus_tag="OO315_003477"
     CDS             complement(5233..6183)
                     /gene="cbl"
                     /locus_tag="OO315_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5892253.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(6285..7202)
                     /gene="nac"
                     /locus_tag="OO315_003478"
     CDS             complement(6285..7202)
                     /gene="nac"
                     /locus_tag="OO315_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5892254.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            7530..7605
                     /locus_tag="OO315_003479"
     tRNA            7530..7605
                     /locus_tag="OO315_003479"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7563..7565,aa:Asn,seq:gtt)
     gene            complement(7661..8596)
                     /gene="ldtA"
                     /locus_tag="OO315_003480"
     CDS             complement(7661..8596)
                     /gene="ldtA"
                     /locus_tag="OO315_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5892255.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(8658..9737)
                     /gene="cobT"
                     /locus_tag="OO315_003481"
     CDS             complement(8658..9737)
                     /gene="cobT"
                     /locus_tag="OO315_003481"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5892256.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(9749..10492)
                     /gene="cobS"
                     /locus_tag="OO315_003482"
     CDS             complement(9749..10492)
                     /gene="cobS"
                     /locus_tag="OO315_003482"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5892257.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLLGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(10489..>10953)
                     /gene="cobU"
                     /locus_tag="OO315_003483"
     CDS             complement(10489..>10953)
                     /gene="cobU"
                     /locus_tag="OO315_003483"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5892258.1"
                     /translation="LYIATSQILDDEMAARIEHHRQSRPEHWRTVERWQHLDELIHAD
                     INPNEAVLLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAK
                     VVLVTNEVGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT         3042 a         2785 c         2372 g         2754 t
ORIGIN      
        1 aaatggtagt gtccgctatt gccagtacac ctcaatgttt ggtgccagtt atcgctacag
       61 tggtgctgga gtcggttatc aggttgccag tgtggttggc ggtggattta caccttttat
      121 tgccgctgca ctcatcactt actttgccgg gaactggcat agcatcgcca tttatttgct
      181 ggctggatgc ctgatttctg caatgaccgc tttattgatg aaagacaatc aacgctcgtg
      241 atagcctggc gaagatcatc cgatcttcgc cttatacttt tgtttcacat tcctgtgaca
      301 tactatcgga tgtgcggtaa ttgtatggaa caggagacac acatgaataa taagggctcc
      361 ggtctgaccc cagctcaggc actggataaa ctcgacgcgc tgtatgagca atctgtagtt
      421 gctttacgca acgccattgg caaatatatt acaattggcg aattacctga tgaaaacgcc
      481 cgtaaacaag gtctttttgt ctatccatca ctgaccgtaa cctgggacgg tagcacaaca
      541 aatcccccca aaacgcgcgc atttggtcgt tttactcacg caggcagcta caccaccacg
      601 attactcgcc ctactctctt tcgttcgtat ctgaatgagc aactaacgtt gctttatcag
      661 gattatggtg cgcatatctc tgtgcaaccc tcgcaacatg aaatccctta tccttatgtc
      721 atcgatggct ctgaattgac acttgatcgc tcaatgagcg ctgatttaac tcgctacttc
      781 ccgacaacag aactggcgca aatcggcgat gaaactgcag acggcattta tcatccaacg
      841 gaattctccc cgctatcgca ttttgatgcg cgccgcgtcg atttttccct cgcacggttg
      901 cgccattata ccggtacgcc agtagaacat tttcaaccgt tcgtcttgtt taccaactac
      961 acacgttatg tggatgaatt cgttcgttgg ggatgcagcc agatcctcga tcctgatagt
     1021 ccctacattg ccctttcttg tgctggcggg aactggatca ctgccgaaac cgaagcgcca
     1081 gaagaagcca tttccgacct tgcatggaaa aaacaccaga tgccagcatg gcatttaatt
     1141 accgctgatg gtcagggtat tactctggtg aatattggcg tgggaccgtc caatgctaaa
     1201 accatctgcg atcatctggc agtgctacgc ccggatatct ggttgatgat tggtcactgt
     1261 ggcggattac gtgaaagcca ggccattgga gattatgtcc ttgcacacgc ttatttacgc
     1321 gatgaccacg ttcttgatgc ggttctgccg cccgatatac ctattccgag cattgctgaa
     1381 gtgcaacgtg cgctttatga cgctaccaag ttagtcagcg gcaggcctgg tgaggaagtc
     1441 aaacagcggc tgcgtacagg tactgtggta accacagatg acaggaactg ggagttacgt
     1501 tactcagctt ctgcacttcg ttttaactta agccgggccg tagcaattga tatggaaagt
     1561 gcaaccattg ccgcgcaagg atatcgtttc cgcgtgccat acgggacact actgtgtgtt
     1621 tcagataaac cgttacatgg tgagattaag cttcccggcc aggctaaccg tttttatgaa
     1681 ggcgctattt ccgaacacct gcaaattggc attcgggcga tcgatttgct gcgcgcagaa
     1741 ggcgaccgac tgcattcacg taaattacga acctttaatg agccgccgtt ccgataataa
     1801 gttatgaacc ttcttatcag aaatagatgt gaaggattaa gtaagacatc tggcaaatag
     1861 cctgcaataa acgcgggctg cttgcttcct tgggcagatg cgagttttat tatcgtctta
     1921 atgatttcca catattaaaa gcaagtatgc tttcaaaaca caattataaa aaatcccgcc
     1981 aacaacatta gtttttataa aattaaatat aagattatgg ctttagaata tttttatttc
     2041 taatagacga gatttttcct gttatgatat aatatgccga attaacacat gttaacgatt
     2101 taccagtaat gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat
     2161 attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt
     2221 gtagaaatct atgctgctgc caaacaaggt gaacccgatc cagaattaaa cacatcttta
     2281 aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca
     2341 attgataaag ccaaaggcgg cggagatgaa acgttcgtac agggacgtta tgaaggcttt
     2401 ggtcctaatg gctcaatgat tattgctgag acattgactt caaatgttaa ccgtacgatt
     2461 gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc
     2521 agctatatgt ttgacaatac gggtgtgatt gtatttaaag gaacagaccc tgaccatatt
     2581 tttgaaattt tgcttgaagc tgaagttgat gttcgtgatg taactgaaga agaaggtaat
     2641 attgttattt atactgaacc tgctgacctt cataaaggaa tcgcggcgct aaaagcagct
     2701 ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt
     2761 tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat
     2821 gtacaaaaag tttatcataa cgtcgcaaat ctctaattat attttaaaga aatctgtctt
     2881 tatggcagat ttctttaatc tcatataatt cttataaaaa atataatatt taactcgtca
     2941 tattgcttat aaaaaatccc cgtccccaga gaaataatat ttattaaaat ggcaggtctt
     3001 ctttttctga ttaccgaaga caaagtggca attacgtata gtttcccgat aaagacgcga
     3061 cagctacgtc ccgcataagg catttttctc tttatctttg tacggtactt catggaacag
     3121 aatttttgac cttgtgaatc gtgatatctg ttggggaggg gcaatttgct cactgaagcg
     3181 tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggccact tttttacaaa
     3241 gtggccgggt aaaaataatt tttatttttt aactgtcctg agtctcatag agatgtctca
     3301 aaactaaaat ttggctcctc tgactggact cgaaccagtg acatacggat taacagtccg
     3361 ccgttctacc gactgaacta cagaggaatc gtgagaacga ggcgaatatt agcgatgccc
     3421 acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt ttgctgaaat aatctgcatt
     3481 ttgtcgttta ttccgacaca actggctttt tttcacactt ttgcggctcg gatcgagggt
     3541 atttccacag ccaacgtcca gtaaccattc gccagtaaaa cagcacggcc cgcacagccc
     3601 agtcggcaaa cattcccatc cagacaccaa ccacacccca gccaagcatg attccctgca
     3661 cataaccgac tacaacccgg caaccccaca tgctcaacat agaaacccac atggcgtaac
     3721 gggcatcacg agcaccctta aatccagcgg gtagcaccca tgaggcggac caaataggca
     3781 taaataaagc atttagctaa atcagaatca caacgacgtg tttaacctgt ggatcctggg
     3841 tgtaaaacga tgccataacc ccggcaaagg gagccgttag ccaggcgatg gccgttaatc
     3901 caagagtgga cagccagaac acatgccgca actgaatctc tgcttgcgct atctgcccta
     3961 cccccaacct tcggcctgta atgatcgtag aagcagaacc gagcgcactt ccgggtaagt
     4021 tgataagagc cgcaattgag aacgcgataa aatttccggc aataacgctg gtccccatcc
     4081 cggcaacgaa catttgggtt aataaccgac cactggtaaa taacactgat tcgacactcg
     4141 ctggaatacc aatccccatg acttcccaga taatgctaaa attcagcggt ttaaaatagc
     4201 tctttaacga aatccttagc gcaggattaa aaccaatcgc cagcacccac aaaattgcaa
     4261 ctgcgccaat ataacgagaa atggttaaac ccaacccagc cccgaccagg agaaaaggcc
     4321 gtaaatcaat atgccgctaa taataatatt aagaatattc aggctaccgt taatcagtag
     4381 cggtattttc gtattccctg caccacgaag tgccccgcta ccaataagag tgatggcagc
     4441 ggctggataa ctgagtacgg tcagctccag ataagtcaac gccagtgctt taacttctgt
     4501 cgtggcatca cccgcgacga aatcaataat ttgttcgcca aaatgatgaa ttagcgttgc
     4561 caacagtacg gcaaacaacg tcatgatcac caatgactgc cgcgtcgcca ccctcgctcg
     4621 tcgtcgatcc cgcttaccga gactaaatgc tacaacgaca gtagtaccaa gatcgatagc
     4681 tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc aaccccacgc cggccatcgc
     4741 atcttttccc agccaactga ccagaaaagt gctcaggacc cccatcaaca ggacacaggc
     4801 gttctccatg aagataggaa cagcaagcgg ggttatctcg cgctagaaca acactttgta
     4861 gctcttgcgt ttagagtgcc agcgagtgcc gtgaacaacc tggcgtaaag cagaggagat
     4921 attcaaagcc gaccttaatt gcagaaagtg aaaccacatt tcaaatgatg agggagaatc
     4981 agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa atgcaacaaa ctgttgatag
     5041 aaacggcaaa cagttgggaa atttaaaaat cgggtttgac aaaatatttt tcgccgttaa
     5101 gatgtgcctc aacagcgatt cctctgtagt tcagtcggta gaacggcgga ctgttaatcc
     5161 gtatgtcact ggttcgagtc cagtcagagg agccaaattt ctgaaaagcc cgcttttata
     5221 gcgggctttt tgctatatct gataatcaat ttcctcttca ctgttttcca tcacctggcg
     5281 cttgatatcc tcaactgaca gtcctgcatt acaaagttcc agaaagcgcc aaacatagtt
     5341 acgctgaagt tgtcctcgct tcagtcccaa ccagacagta ttagcatcga aaagatgccg
     5401 cgtatccagg cggattaaat tctcttcctc ttgttcgccg ctggattgtt cggcaactaa
     5461 tccgatccca agcccaagag caacataggt tttaatgaca tcagaatcct gtgcacttaa
     5521 tacaatatct gccggcaaac ctttgcgggt aaatgcgtca tcagtgcgtg agcgccccgt
     5581 aattccctgt cggtaagtaa ttaacggcca cttcgctatt gattccagcg tcaatggtgt
     5641 aatttgcgtt aagggatgat cgagtggaac aagcaaacta tggtgccaac aaaaccatgg
     5701 gaaggcgacg agctgcgggt cattactcaa acgctcgctg gcgataccaa tatcagcttc
     5761 gccattttgc aacaacgtcg caatttcctg tggcgaaccc tggattagct cgagccgaac
     5821 ctccgggaaa agttcgcgaa aagctttaat gacctctggc aagctataac gtgcctgagt
     5881 atgcgtcgtt gcaatagtga gaacaccaga cgtatcgttg gtaaacaggt ctgcaagccg
     5941 acgaacatta ctggcttcat tcagaatacg ttctgcaatg accagtaatg ctttgcccgg
     6001 ttcagtcatc cccagcagtc gcttacctcg tcgaacaaat atttcgatgc caaattcatc
     6061 ctccagttcc cgaatatgac ggctaacgcc tgactgtgag gtaaaaagca tattcgcaac
     6121 ctctgtcagg ttgtaatcct gacgtgcagc ctcgcggatt atctttagtt gttggaaatt
     6181 cacggtaaac tccgggcagt tcagatatcc cgttattgtt aaagtctaat gcccggcata
     6241 acaaataata aaaacccgca tcttattcca tcccgatata acgcttagct caccaattgc
     6301 cactgccttt tttccatcac tggagaacta atcactgaca ttaacaactc tttcactgcc
     6361 tgtgcctgtg gtgacaagtt tgctctggcg ggtaaattta aggacaaaga gagactcatt
     6421 gaaggagtgg taatgcgtga catccaccca tttactgcgc cacataacga acgcgcagcc
     6481 gattcaggta atactgcaac gcccatgccg ctggcaatcg ctgcggtaag cgtggcaata
     6541 gactcaattt caccaatgac ttttgccgtg agccgccgta gggaaaaagc ctcatcaaca
     6601 cgaagtctaa cagcgctgta atcactgggg agaaaaaggt tcatttgcgc aatagcatta
     6661 acatcaacgt tttgccccgg gcaatcttga gttcctacca ggaaaagatc ttctttcagc
     6721 aaagcctgac tggatacccc agccacaggg gaatgctcat aaatcaccgc catatcgagt
     6781 tggtgattta tcagtttttc gttaagcact gcaccactat tttcatgaag atagataacg
     6841 acctccggga attcagcgcg aaccgcctgt aataagggca tggtgatgga tgacgcagcg
     6901 gttcctggcg caaagccaat cgagacttgc cccgataatg actgaccaac gttatgcacc
     6961 gccagttggg cctgctcaca ctgacgtaaa atggcccgtg catgggtata gagaattttt
     7021 ccggcgtctg ttggcgtaac gccccgtttt gtgcgaatca aaagctgttg atttaactca
     7081 ccttccagta tggcaacctg ttggctgagc gctggttgtg cgatatgcaa tacttcagca
     7141 gcctgggtca ggctaccaat atctacaatt tttacgaagt atttcaggcg tctgaagttc
     7201 atgttgcctc cggtttttaa gaatcggccc aagtgccgcc attacttaca accagtatgc
     7261 aagatgcctg ccagttctat tttggcgttg atgcgcaagc taaccaactg tcaaataaga
     7321 gattatgata gattcgtcat tggccctttt tatcagcagt cgcattcacc tgccctataa
     7381 aaggagggta tgcaccacga tggttcatta cccaataaga ttgaaagctc accactttgt
     7441 tgaaattggc agcaaacaaa caaaaaaaat gcatttcacc ctttgacatc accatgcact
     7501 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     7561 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     7621 gctttcgagc aggcttttta ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     7681 cagaactaat ttgcacaggc attcccgatc gatgttgcaa cgcagcattt gcgcgattta
     7741 catcaacttc ttgcccgttg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     7801 ttcggtcaga ctcatattct gcacgattgc gcgacagcgg ctcatgaact tccagccact
     7861 ttgaaccatc cggttccgtt gtgtatttca ctggctgatc aataatttgt acacgagtgc
     7921 ctacaggaac attatcaaac agatatttga tatcgtcatt acgcagacga atacagccct
     7981 gacttacacg gagcccaata ccaaaattgg cattggtacc gtggatggca tacaacctgc
     8041 caatataaat cgcgtacagc cccatggggt tatcgggccc cgcaggaaca aatgcgggca
     8101 aactctcccc tcgtttcgca tattcgcgcc ggatgttcgg cgttggcgtc caggttggtg
     8161 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     8221 cgataccaat aggaaagact tccactgtat tactgtctgg cggatagtaa taaagacgca
     8281 tttcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     8341 gaatggtgag ttgtgagcca ggcttcggca aaaaaacatc agcgcccgga ttcgcttcca
     8401 gcatattact taacccttgc ccgtattgtg cggcaaaagt ctccagcggc tgagtattgt
     8461 gatcaggaac agttacagta agcgactgcc ccactaaacg gctaccctct ggaggtaatg
     8521 gataagttac cgccaggcta ttttggctgg caaaaagcag agcaaatgag caaagaatat
     8581 ttacacgacg catcatgtcc ctttcctaag tcgcgaagac tatccgataa gtatagcttt
     8641 tatcagactt ttcgttttta gctgttcaaa tcagaagtcg tattccccgg tagaacaata
     8701 ttactggcag caagttcgcc catgttgttg tatatcgcac aagcagcttc gatgatggac
     8761 atcgccagag ctgcaccact cccctcacct aaacgcatat ccatattgag ataaggctcc
     8821 agccccaaat gcgagagcgc tatacgcgcg cctttttctg ccgacaagtg agaaggaatg
     8881 agatacggtt tgattgcagg agacatctgg caggctgcga gcgcagcagc ataagaaaga
     8941 aatccatcca gcaacacggg taaaccgcag gaagcagcac ctaacatcac tccagctatt
     9001 ccgaccaaat caaatccacc cacttttgcc aggacatcaa caccatcctg aggatttggt
     9061 tgattcaacg taatcgcccg acgcacaaca tcaattttat tagccagttt atctgtcggc
     9121 aggtttgcgc caatcccaac cacttcttca ggatcccggc cagtgattgt gctgactatt
     9181 gccgctgccg gtgtcgtgtt tgccatcccc agttcaccta caccaaacag cgtgacaccg
     9241 tttttcgcca gctcccgcgt ataacatatg acgtccaaaa gcaacttttc agcctgacgg
     9301 cgactcattg ccggagctga agcaatattg ccgctacctc gtgcgacacg catgttgata
     9361 agcccgggga taggctcagc agtatcaata ccaacatcaa ttacgtggac gttagcgccc
     9421 gcttgtgctg ccagcacaca cacgccggtt gttccacggg tcatattttc agcctgtatg
     9481 gctgtcactt cttttggaga aatagcgacc ccttcctccc agacgccgtg atcggcacac
     9541 ataaccagta ccgctttttt gcccacatgc ggtatgccat tcacccccgg cattcctgcc
     9601 agttgtatgg caagcgcctc cagctttcct aggctaccaa caggtttgaa taacccgtca
     9661 acatgccgtt gtgcacgcga catagcggca gaattgatgg ctgggatcgt attcagtaaa
     9721 tcggcaatta tttgcatgtc acgtttcttt atagcagtgc cagcaggaag accagttcac
     9781 caagctctat ggctgctccc agcgtatcgc ccgtttgccc gcccagcgta cgtttgagaa
     9841 gctgaccgag gatgaaaatc gccaccatcg tcaccaccat agcagccaca ccatgcatac
     9901 ccagcaataa tacggcagca aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc
     9961 catcaatttt gccaatgaac acattgccaa gcccttcttc ccgcgcataa cgatgacgat
    10021 acatcaataa tgcggcagtc ccgcggctga ccgcacatgc tgccgccagc gatgcaagga
    10081 tcggttcgcc acgcagagcc aactcgctta acaccagaat ctttgccagt accacaaaaa
    10141 tcaatgccag accgccgtgg gtgcctaaac ggctatcacg catgatttcc aacatgcgat
    10201 cgcgactacg tgcagaaaat acgccatcac aggtatcagc gaggccgtcc agatggaatt
    10261 ccccggtcat cagcgccagc acaagtacgc taaacagtgc cgccagtggt gcgccacacc
    10321 atgcctgcag caccatgaag accagcccgc taatcgcgcc aagcaataat ccaatcaaag
    10381 gaaaagtaat aataccgcga gaataatgtt cgaaatccag tccctgggac cagcgacgcg
    10441 gtacaggcag acgcgtaata aatgagagca tcgcccaaaa taatttgctc atttgatttt
    10501 tactccaata cccgaaacca ccagccatac ttcatttgcc gcagcggcca actgctgatt
    10561 tacccgcccg gcaatatcac gaaaatgtcg tgccagacga ctctccggca caatccccat
    10621 tcccacttcg ttagtcacta atacaacctt tgcggggcaa cgttggcagg cagcaatcaa
    10681 cgactgaatc tcagcattaa tcgcctgttc catcgcctga taatcccatt catcagggtc
    10741 tttatcgccg ccataatcaa acaacagatt agtcaccatt gtggtaacgc attccagcaa
    10801 cacagcctca ttcgggttaa tgtctgcatg aattaattca tcaagatgtt gccagcgctc
    10861 cactgtgcgc cagtgctccg ggcggctttg ccgatgatgt tctatccgtg cagccatctc
    10921 atcatcaagg atttgcgagg tagcgatata cag
//
LOCUS       DAJTRD010000118        10930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTRD010000118 DAJTRD010000000
VERSION     DAJTRD010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.118"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            157..507
                     /locus_tag="OO315_003484"
     CDS             157..507
                     /locus_tag="OO315_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5892259.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            560..955
                     /locus_tag="OO315_003485"
     CDS             560..955
                     /locus_tag="OO315_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5892260.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            996..1739
                     /gene="cmoA"
                     /locus_tag="OO315_003486"
     CDS             996..1739
                     /gene="cmoA"
                     /locus_tag="OO315_003486"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5892261.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1736..2707
                     /gene="cmoB"
                     /locus_tag="OO315_003487"
     CDS             1736..2707
                     /gene="cmoB"
                     /locus_tag="OO315_003487"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5892262.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2872..5301)
                     /gene="torZ"
                     /locus_tag="OO315_003488"
     CDS             complement(2872..5301)
                     /gene="torZ"
                     /locus_tag="OO315_003488"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5892263.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(5326..6426)
                     /locus_tag="OO315_003489"
     CDS             complement(5326..6426)
                     /locus_tag="OO315_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5892264.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(6814..7560)
                     /gene="cutC"
                     /locus_tag="OO315_003490"
     CDS             complement(6814..7560)
                     /gene="cutC"
                     /locus_tag="OO315_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5892265.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(7574..8140)
                     /locus_tag="OO315_003491"
     CDS             complement(7574..8140)
                     /locus_tag="OO315_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5892266.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            8356..10089
                     /gene="argS"
                     /locus_tag="OO315_003492"
     CDS             8356..10089
                     /gene="argS"
                     /locus_tag="OO315_003492"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5892267.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10266..10754
                     /locus_tag="OO315_003493"
     CDS             10266..10754
                     /locus_tag="OO315_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5892268.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2670 a         2791 c         2605 g         2864 t
ORIGIN      
        1 ccataatggg cttttaatcc tatcgtttat tgcttgcata gtagaatcta accatccatc
       61 actcatagct aaccctcgga aatcttacat ttctgtctat cataaccact agcatcacct
      121 ttaggaacca tctgaaacgg gaggtgtatt tagaccttgc taatcttaga cagccgcccg
      181 gttcatgcat cacgtccaca cagtgaagct attcgcgacg ctcaacgaaa aaaacctaaa
      241 gttccggtac atgctgtact gacggcgaca aatccgctga tccgttttat cggtagtgat
      301 gatatgacgc aaaaccggga attatttcag gtctggttac aaaaattagc gcagtggcat
      361 cagaccacta cgccttatct ttttttacat acgccagata ttgcccaggc cccggaactg
      421 gtacataccc tgtgggaaga cttacgtaaa acgcttccag agatcggagc agttccggct
      481 attccacagc aatcttctct tttctgaatt tgccacctat catagacagg tgccatcggc
      541 cattttaaag ggagtttgta tggtaagcgc gctatatgcc gttttaagtg cgttgttatt
      601 aatgaagttc tcttttgatg tcgttcgcct gcgaatgcag taccgcgttg cctatggtga
      661 cggtggtttt agcgaactgc aaagcgctat ttgcattcat ggtaacgcgg tggaatatat
      721 tcccatcgcg attgtgctga tgctgtttat ggaaatgaat ggcgcagaaa cctggatggt
      781 gcatatttgc ggcatcgttt tgcttgctgg tcgtctgatg cattattacg gttttcatca
      841 ccgtctgttt cgctggcgac gttccggcat gagcgccacc tggtgtgcgc tgttgctgat
      901 ggtgctggcg aatctttggt atatgccctg ggagttggtt ttctccctgc gttagcgcac
      961 aatacgccac tttctttttc ccggattttt acgttatgtc tcaccgcgac acgctatttt
     1021 ctgcccctat cgccagactg ggcgactgga cctttgatga acgggtcgct gaagtcttcc
     1081 cggatatgat ccagcgttcc gttcccggct attccaatat tatttccatg attggtatgt
     1141 tagccgagcg cttcgttcaa cctggtacgc aggtttacga tctgggttgt tctctgggcg
     1201 cggcgacgct ctcggtgcgt cgcaacattc atcatgataa ttgcaaaatt attgccatcg
     1261 acaactcccc ggcgatgatt gaacgctgcc gtcgtcatat tgacgcctat aaagccccta
     1321 cgccagtaga cgtcattgaa ggtgatattc gcgatatcgc cattgaaaac gcatcgatgg
     1381 tggtgctgaa ttttaccctg caattcctgg aaccttccga gcgccaggca ttactggata
     1441 aaatttatca aggactgaac ccgggcggcg cgctggtgct ttcggaaaaa ttcagtttcg
     1501 aagatgccaa agttggtgaa ctgttgttca acatgcacca cgactttaaa cgtgccaacg
     1561 gttacagcga actggagatc agccagaagc gcagcatgct ggaaaacgtg atgctgaccg
     1621 attccgtgga aacccataaa gcacgcctgc ataaagccgg ttttgagcat agcgagctgt
     1681 ggttccagtg ctttaacttt ggttcactgg tggcattaaa agcagaggac gctgcatgat
     1741 cgactttggt aacttttatt ctctgattgc caaaaatcat ctttcacact ggctcgaaac
     1801 gctgcccgcg cagattgcta actggcagcg cgagcagcag cacgggctgt ttaagcagtg
     1861 gtccaacacg gtggaatttc tgcctgaaat taaaccgtat cgtctggatt tattgcatag
     1921 cgtaaccgcc gaaagcgaag agccactgag caccgggcaa attaaacgca ttgaaacgct
     1981 tatgcggaac ctgatgccat ggcgcaaagg gccgttctca ctgtatggcg tcaacatcga
     2041 taccgaatgg cgttccgact ggaaatggga tcgtgttctg ccccatcttt ctgatttaac
     2101 cggacgcacc attctggatg tcggctgcgg cagcggttat cacatgtggc gcatgattgg
     2161 cgcaggcgcg cacctcgcgg tggggatcga ccctacgcaa ctgtttctgt gccagtttga
     2221 agcggtgcgt aaactactgg gtaacgatca gcgcgcacat ttgttaccgt taggtattga
     2281 acagcttccg gcactgaaag cctttgatac cgtcttttcg atgggcgtgc tttaccatcg
     2341 ccgttcgccg ctggagcatc tctggcagtt gaaagatcaa ctggtgaatg agggtgaact
     2401 ggtgctggaa acgctggtta ttgatggcga tgagaacacg gtgctggtac caggcgatcg
     2461 ttacgcgcaa atgcgtaatg tctatttcat cccttccgcg ctggcgctga aaaactggct
     2521 gaagaagtgt ggttttgtag atatccgcat tgtggatgtg tgcgttacca ctactgaaga
     2581 gcagcgacgc actgaatgga tggttaccga atctctgtcc gatttcctcg acccacatga
     2641 tccgagtaag acagttgaag gttatcctgc gcctaaacgc gcggtgctga ttgcgcgcaa
     2701 gccgtaaagg tctggtatta ctgccggatg cggcgtgaac gccttatccg gcctacaaac
     2761 ccttgctaat tcaatctatt gcagggacta tgtaggcctg ataagcatag cgcatcaggc
     2821 agttttacgt ttgcataacc tcagcgcccg tttccgggcg ctattcactt cttacgcctg
     2881 aacagcaggc ttatcaaatg ccgttaactt cggtgcgtta cccgtatatt tttcgatata
     2941 caccagcgca gagttaccgg cacaggcatt tgcccgctgc gagctgggga tatccgccgt
     3001 taacacgttc gcactgccgt ttttacacaa gccgttttcc agatctggcc atgcaccttc
     3061 atgcacgcat accacgcctt ttttgatccc gtcagtcacc accgcgcctg tcaaaatctg
     3121 accacgtttg ttccagacac gcaccagatc gccattcgca ataccaaatc gagcagcatc
     3181 ttcggtgtga atagtgatcg gttcacgatc ggcaaccgca tattttttac gcagttccgc
     3241 atagttaagc tgactgtgta aacggtgtgc cggatgtgcg gtcagaagct gcaactgctt
     3301 ctcgtcggcg gtacccttcc actcatcagg cgcaagccag gttgggtgtg ccgggcaatc
     3361 cttatagcca aatttttcca gcgttttgga gtaaatctca attttgccgc ttggcgtacc
     3421 cagcgcattt ttcaccggat cggcgcggaa atcaccataa cgaacgtact gttcgttctt
     3481 ctcgctgcgg cgcatttcga tcagtttatt ttgctgccag aaggcattaa acattggcat
     3541 agtgacgcgt tgcgcacggg cccctttctg agcggcatca tagaaaaatt tcagccacgc
     3601 catttcatct ttaccttcgg tatagatctc ttttccgcca ggtttgagta attccgcaag
     3661 atcggcaaac acgtcaaaat cgttacgcgc ttcaaattgc ggagcaacag cctgcttcat
     3721 cggcacaata tgctggttgc tgtaatcacc ggtcatcgtc aagtcattgc gctcaaacga
     3781 tgtggtgatc ggtaatacga tatctgcgtg tttggctgct gcggtccagt agcattcaga
     3841 aacgacgatc atctccggtt tctgccaggc tttgatcaga cgattagtat cctggtgatg
     3901 ggtaaagtta ccgccacccg cccaccagat cattttgata ttcggatacg tttgttcttt
     3961 accgttatgc tgatatttgc cgccaggatt ttccagcgca tcgacgatac gggcaacagg
     4021 aatcgccgtc attccgccat catcagcggc ctcgctggcc tgtccggcaa tcgccgcaga
     4081 catttccggc agcacgccgc caacacgcgt cgggttgcca ccattggagt aatgataaga
     4141 gaaaccaaaa ccgccgcccg gtgtaccaat ctgccctaac atcgccgcca gcgtcaccag
     4201 catccagtgt ttctgttcac catattgctg gcgctgaatt ccccagcctg ccatcagcat
     4261 tgtgcgattc gccgccatca gttcagcgag tttgactatc tgcgcttccg gtacgccagt
     4321 aatttccgcc gtccagaccg cgctcttcgg cgtattatcg ctcttacctg tcagatactc
     4381 ttcaaactgc ggatacccgg tagtgtattt ttcaaggaac actttatcgt gtttgccttg
     4441 tgtcatcagg gtatgcgcaa tccctaacat cagtgccacg tcggtgccca tattcggcgc
     4501 gatccaggtg gcattatcgc caaagaattc gatggtttcg gagcggatag gatcaatggc
     4561 aatcactggt ttgccagatt ttttcagctg atggaagtat tccagccctt gctcatcggt
     4621 actgctccag gcaattttta aggtattcag cgggttcatt ccccacagca ccacaacctg
     4681 gctgttttcc agaatcagcg gccaggaggc ctgctgttca tacacctcta cagaaccgac
     4741 cacatgcggc atgatcacct gtgctgcccc ggttgaataa tcgccgctat gcccggaata
     4801 accgcccgcc aggttcatat aacgttgcag taaggtttgc gctttatgca acacgccaga
     4861 agagcgccag ccgtaagatc cggcaaaaat ggccgatggt ccgttagctt tacgaatacg
     4921 ctcatgttgc tcatgaatca gttttaatgc ctgttcccag ctcacctgta cataggtatc
     4981 ttcgccacga cctttcgccg gttgcagtgg attgtcgaga tagctttttc tcaccatcgg
     5041 atgctgaata cgcgccgtgg tgtgtacctg atccgccgcc gtagactgta aggagttcgg
     5101 tatggttttc gccagcgcgc ctgtcgaaga aacaatcttg ccgtccttca cttctacgct
     5161 catcgctccc caacgtcccg cggtgaggat tttaccgccc ttctcttctg cccatgcggg
     5221 gagcggtgct gccgatgtca ccaccagcgc tccagcggca ataccgctgt gtttaataaa
     5281 ttcacgtctt gttaatgcca taacttcctc cctgatcaac gaggatcact gtttctcggt
     5341 aatatctttg gcgttgtact ggaaataccg cgttaaaatg tccggttcgt tttcgctcat
     5401 gctggttcgt gcccccattc ctttggcaat ggacggccac gcattgacgg tgtaatggtc
     5461 ggcggcaata gaggcatgac aaccagcgca ataggtatcg tcaagttttt cagcgtattg
     5521 ccatagcggt ttacggtccg ctaatgcggg atcggtaagc gcaccctgta aagacgcctg
     5581 acgccattga ttgccgtatt cgtcagcctg ccattccccg tttaccgtga gcgccttgat
     5641 accttcttca cttaatgtgg ctagcgccag ccgttgacct gccgccaggt agagcgtgtt
     5701 ttcactgccc tgcatttgat aaccctgcaa cagaacgatc ggctgtttgc cactggcatc
     5761 aacgacggtg agatcggtac caggattcac ggtagccagc tcgcctatgc gagaagtttt
     5821 gaaaggataa atatgtgcgc cattagtcac tgaagtagcg gcctgacttt ccagctcatg
     5881 cgccgcgttg tcatccattt ttatttctgg cggaaaatgg gcaatgcctt tatgacaatc
     5941 gatacaggtt tcgccgccct tttgtgcttt gttatgcatt ttctgcgcag attcactttg
     6001 cgaggcaata tccatggcat caaaagaatg gcaactacgg cacgttgcag agtcagttgc
     6061 ttttaattct ttccatactg tttcggccat ttcctggcga tgagtttcga acttatcgtc
     6121 actgtctatt ttgccgctaa caaattcatg ataaatatct ttagatgctt ttaatttagc
     6181 aaataaataa tccatccctg actttggaat atggcaatcg gcacattccg cacgtatccc
     6241 tttctggttc gaaaagtgga cagttccctg atattcctca aaaggtttac tcatcgagtg
     6301 gcaggaaagg caaaatgctg tatccgacgt tttatgtaag gctttttgcg ccagcaataa
     6361 cccgccgcca ccaaccacaa ccgctatcag caaaaacaat aacccaatgc gttttttccc
     6421 tcgcatagat tcctctaaat aaattccaga acaccgaaat cattgtataa ttaaatacac
     6481 aacgaattaa ccgcttgcat aactcggcac aacactgcct gaaataatcg ataaagaata
     6541 tgatttatta caatgtaatc attaattgct aaggaataac ccagttgcta tttagaatat
     6601 atatttatca gttttagcaa tttaaatccc ataatcaatg tgaatataaa aaatattttt
     6661 agcacaacgc tatgcgcact gtcacccgct caacagatca cggttatcgt tcgtttttta
     6721 tactgctcag ggataaaaaa aggcccctgt tgaaattgca ggggcctggt acgagcaagc
     6781 atcatattgg gcgacatgat gcaacggtaa aaatcatttg gcctgatggc gttcaatgat
     6841 tcctttcatt tcagcaaccg ccgccccgtc tacgatataa cgcgaatact cgtccgcgtg
     6901 ttcatctgat gacatggaca atccttgatt acgataacgc atcggtgagg cttgccacgc
     6961 tcccgcggag ctatggactt ccagcactcc ggcatcgagg aagtggtgca ggttttctgc
     7021 acggactcct gctccagcca taatgattgg agcatcacga tgggcaataa gttccataat
     7081 ttttgataaa ccttgcagtg cgtctgattt ttgccctgat gtcagtaccc gggcaatgcc
     7141 caattccgca agattattga gtgtatataa agggttagcg cacatatcga aggcgcgatg
     7201 aaaagtcact gccagcggac cggcagcagc cattattttt tccattcgtg gcatatcgac
     7261 attcccgtca acatcgagaa cgcccgtcac cagtccagga aaacctaatt cgcggaccgt
     7321 gcgcacatcc tcaagaatgg cggcaaactc accgtcgctg tagcaaaaat caccaccgcg
     7381 tgggcgaatt atcggatgca cagggatcgt cacccgctgg cgcacggatt tcagtacccc
     7441 caacgacggc gttaagcccc cctcttttgg ggctgcgcat aattcaactc tgtctgcgcc
     7501 gttttgctgc gccgttagtg cacattccat gctgtaacag caaatttcca gtaatgccat
     7561 ttttgctcct taattacgct gactgctcgc tggcaacgat ctcttcaatg gaccacggat
     7621 gaaacttaat agttgttttg ccatcggtga ccgccagcgt cggattcggt aatcgctcat
     7681 gctcaccttt tggtgaactg gttttaactg agatccccgg taggctgagt ccttcatcag
     7741 aaagcaatgc cagcgcacgg gcattcagcg tttctggatc acccggcaga acgatttcaa
     7801 tatgttccca accttcgtgt gggtaacgtt tttccccggg ccacggtagc tccacattgg
     7861 aaaactgcca gtgcgcaacc tgtaccggtt catgcaattt aaacagacaa atcggtctgc
     7921 cattgatcat tttttctgac aaaagctcgc cacactgttc aaaaccgcga cgccagcgct
     7981 cagcggtgac gttttgatgg caacgcaaag aaatgtgatc ggcagtcagc ggagtgatat
     8041 ccaaccccag acggcgggaa agttcatcta atgcgtgggt aaatcgcggt aaatccgatg
     8101 caatatcctg cagctcgtcg atagattgcc agttcgccat aatcactctt cgtctttcag
     8161 taaaagcgtt aatttaccct gttgccctgt gccaaccaac cgctgatttc acgccgcttc
     8221 tgatgcaata gtgaaaacgg caatacgcca cgcgcacgtt gctgacgaaa acagccattt
     8281 gcagtatact cccgccctaa tttctttaac tggtgcgggc aatttttgct cgcttcatca
     8341 atgtaaggta ttccggtgaa tattcaggct cttctctcag aaaaagtccg tcaggccatg
     8401 attgcggcag gcgcgcctgc ggattgcgaa ccgcagattc gtcagtcagc aaaagttcag
     8461 ttcggcgact atcaggctaa cggcatgatg gcagttgcta aaaaactggg tatggcaccg
     8521 cgacaattag cagagcaggt gctgactcat ctggatctta acggtatcgc cagcaaagtt
     8581 gagatcgccg gtccaggctt tatcaacatt ttccttgatc cggcattcct ggctgaacat
     8641 gttcagcagg cgctggcgtc cgatcgtctc ggtgttgcta cgccagaaaa acagaccatt
     8701 gtggttgact actctgcgcc aaacgtggcg aaagagatgc acgtcggtca cctgcgctct
     8761 accattattg gtgacacagc agtgcgtact ctggagttcc tcggtcacaa agtgattcgc
     8821 gcaaaccacg tcggcgactg gggcactcag ttcggtatgc tgattgcatg gctggaaaag
     8881 cagcagcagg aaaacgccgg tgaaatggag ctggctgacc ttgaaggttt ctaccgcgat
     8941 gcgaaaaagc attacgacga agatgaagag tttgccgagc gtgcacgtaa ctacgtggta
     9001 aaactgcaaa gcggtgacga atatttccgc gagatgtggc gcaaactggt cgacatcacc
     9061 atgacgcaga accagatcac ttacgatcgt ctcaacgtga cgctgacccg tgatgacgtg
     9121 atgggcgaaa gcctctacaa cccaatgctg ccaggaattg tggcggatct caaagccaaa
     9181 ggtctggcag tagaaagcga aggggcgacc gtcgtattcc ttgatgtgtt taaaaacaag
     9241 gaaggcgaac cgatgggcgt gatcattcag aagaaagatg gcggctatct ctacaccacc
     9301 actgatatcg cctgtgcgaa atatcgttat gaaacactgc atgccgatcg cgtgctgtat
     9361 tacatcgact cccgtcagca tcaacacctg atgcaggcat gggcgatcgt ccgtaaagca
     9421 ggctatgtac cggaatccgt accgctggaa caccacatgt tcggcatgat gctgggtaaa
     9481 gacggcaaac cgttcaaaac ccgcgcgggt ggtaccgtga aactggcaga cctgctggat
     9541 gaagccctgg agcgtgcacg tcgtctggtg gcagaaaaga acccggatat gccagctgac
     9601 gagctggaaa aactggctaa cgcggttggt attggtgcgg tgaaatatgc ggatctctcc
     9661 aaaaaccgca ccacggacta catcttcgac tgggacaaca tgctggcgtt tgagggtaat
     9721 accgcgccat acatgcagta tgcttacacg cgtgtattgt ccgtgttccg taaagcagaa
     9781 attaacgaag agcaactggc tgcagctccg gtaatcatcc gtgaagatcg tgaagcgcaa
     9841 ctggcagctc gcctgctgca gtttgaagaa accctcaccg tggttgcccg tgaaggcacg
     9901 ccgcatgtaa tgtgtgctta cctgtacgat ctggccggtc tgttctctgg cttctacgag
     9961 cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta acagccgtct gaaactggca
    10021 caactgacag cgaagacgct gaagctgggt ctggatacgc tgggtattga gactgtagag
    10081 cgtatgtaat cgatttttcg tgagagtgaa gcctgatcag ggttagccga tcaggctttt
    10141 ttattgccat ctaaatgtat tctgaaaacg gacatgccat tgttttctca ctgttgagta
    10201 agaggccaga agcgtaatat ccaggcccag gaaaacgata acggttcaat ttaaggaata
    10261 ccgaagtgtt taaatttctt gtattaacat taggcattat ctcttgccag gcttacgcag
    10321 aagatacggt tatagtaaac gaccatgaca cttcagccat caaagattgt tggcaaaaaa
    10381 attcagatga tgatactgac attaacgtga tcaaatcatg cctgcgacaa gaatacaatc
    10441 tcgtcgatgc gcaattaaat aaagcctatg gtgaagctta tcgttatata gaacaagtgc
    10501 cacgcacagg tgcaaaaaaa cttgatactg aacaacttaa cttgcttaaa aaatcacagc
    10561 gagcctggct ggattttagg gacaaagaat gtgaattaat cctttcaaat gaggatgttc
    10621 aggatttaag tgacccctat tctgaatcag aatggctatc atgtatgatc atacagacca
    10681 atacgcgaac tcgccagttg cagctatacc gtaactctga agatttttat ccaagccctt
    10741 tgacaagagg ataattcaca tctttttggc atgttttgtt gcaagctatt cctgataaat
    10801 gattgcaaca aaacatccag ccttttgact gagttattaa acatactcgc gagcgcgtaa
    10861 ttttttgtct ttcagcgata attcacaatc acttcattcc gttgtacctt tagcggtgga
    10921 attaaccgcc
//
LOCUS       DAJTRD010000119        10465 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTRD010000119 DAJTRD010000000
VERSION     DAJTRD010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10465
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.119"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            4..204
                     /gene="cspG"
                     /locus_tag="OO315_003494"
     CDS             4..204
                     /gene="cspG"
                     /locus_tag="OO315_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5892269.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            215..403
                     /locus_tag="OO315_003495"
     CDS             215..403
                     /locus_tag="OO315_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5892270.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            378..608
                     /gene="ymcE"
                     /locus_tag="OO315_003496"
     CDS             378..608
                     /gene="ymcE"
                     /locus_tag="OO315_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR5892271.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            598..771
                     /locus_tag="OO315_003497"
     CDS             598..771
                     /locus_tag="OO315_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5892272.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(820..1893)
                     /locus_tag="OO315_003498"
     CDS             complement(820..1893)
                     /locus_tag="OO315_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5892273.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1995..4706)
                     /gene="torS"
                     /locus_tag="OO315_003499"
                     /pseudo
     CDS             complement(1995..4706)
                     /gene="torS"
                     /locus_tag="OO315_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4789..5817
                     /gene="torT"
                     /locus_tag="OO315_003500"
     CDS             4789..5817
                     /gene="torT"
                     /locus_tag="OO315_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5892274.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5790..6482)
                     /gene="torR"
                     /locus_tag="OO315_003501"
     CDS             complement(5790..6482)
                     /gene="torR"
                     /locus_tag="OO315_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5892275.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6569..7741
                     /gene="torC"
                     /locus_tag="OO315_003502"
     CDS             6569..7741
                     /gene="torC"
                     /locus_tag="OO315_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5892276.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDITGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7741..10281
                     /gene="torA"
                     /locus_tag="OO315_003503"
                     /pseudo
     CDS             7741..10281
                     /gene="torA"
                     /locus_tag="OO315_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10278..>10465
                     /gene="torD"
                     /locus_tag="OO315_003504"
     CDS             10278..>10465
                     /gene="torD"
                     /locus_tag="OO315_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5892277.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2526 a         2684 c         2787 g         2468 t
ORIGIN      
        1 aaaatgactg gtttagtaaa atggtttaac gcagataaag gttttggctt tatcactcct
       61 gatgatggca gcaaagacgt tttcgtccat ttcaccgcca tccagagcaa tgaattccgc
      121 acgctgaacg aaaatcagaa agttgaattt tctattgagc aggggcaacg tggccccgcg
      181 gcagcgaacg ttgttacgct ctaaggttgc cgttattact caacatctcc atttccgctg
      241 tccatgttgt catggttcac agtaccgtac atcggcattc gatgtgacgg agcgaaaccc
      301 tttgggcgct aagtgtattt tttgtaaatc gactatgatc acctttgata acgtcgcgct
      361 gcaaatacgc actgaccatg cgccgctgga tttcacaaaa taatatcagg ctccctcgtg
      421 gagccttttt tatatctgcc ttatttttct tcaacgctgt atgtatagta agcgataacc
      481 tgttgattat tgaatctttc gggaagatgg cttataacat ttcttacctg accagggtac
      541 cgggaaccaa caccttactg gcgtgttgct gtctttcaag accagacgag gttaacagtg
      601 aatattgaag agttaaaaaa acaagccgaa acggaaatcg ccgactttat cgcgcaaaaa
      661 atcgccgagc tgaacaagaa tacagggaaa gaagtctctg aaattcgctt catcgcacga
      721 gaaaaaatga ccgggcttga aagttatgat gtcaaaatca aaataatgtg attttgtgaa
      781 catcaccccg tgcgaggtga tgttccgctt gttgctaatt tagtgaccaa tcattggcgc
      841 ttgtggaatt aagcgtcggt acaattcctc cggcaccggg ctttgccata ctcccgcata
      901 cattgcgtaa ccaatcaccg caaacataat ccccagaacc agtagcgtca ttaaccagcc
      961 agacaacgca aaggcttttt tatttgctgc aggtttttgc agtgaaaagg tcaatgttga
     1021 ggctaccgga catgactcta cgcaagtcat acagccggta cattccactg ttcgtacctg
     1081 aattaattta tctaccggga tccgtgatgg gcactttttt gcgcatttgc cacaatcgat
     1141 acaactttcg gcattgcgac gaatcttaaa cggcgacaat agcgaaacca cgcccatcag
     1201 cgcgccatat gggcaaagat aacgacacca ggcatggcga ataaacaggc tggcaatcag
     1261 caaaacggtc acgctgatta atgtcgcggt ccccatatga cgaaagaaat cgagcatttt
     1321 aacgtccatc accacgctat agggcgacaa cataaaatag tgaatcgcct gagcgggcat
     1381 caataacgcg atatagagaa ataaactcaa cagcaaatac ttcacgccgc gcagaggaat
     1441 atccagccag cggggaagga cacattgccg accaaacagt ttgttaccga gatcgccgat
     1501 taattcagaa agcgtaccaa ccgggcataa ccatgagcaa aaggcctttt tgagtaatag
     1561 actgatgacg ataaaagcga ccaataacag catcgcggcg gcgtggacgg acggtaactg
     1621 acctgttgca aggctatatt tcagattcat cagaccggca atcggtagcc agccttcgat
     1681 acctcccggt ctggcgacaa atgtcgtact acctgccgtt tcgtaatagc gcacccaata
     1741 ccagaacgtg atggcaatat aaatattcat tgccaatagt aagaattgcg tcgctttacg
     1801 ccaggtcgtg gcattgcgcc agtcattcca cggtaatttg ccgcccgtcg tgcctggccg
     1861 ccgctgccag cgggttcttt ttttctctgc catgattttg ccagtccgtt aagttgcata
     1921 ccaaatgcca ctattctagt tattcttaac tggctgatat tgattcaaat cgcgttcagg
     1981 tctttcttat tcaatcatgc ttccagagcg gcaacactgc gtgtaatttc ttcgtgtgga
     2041 aggggggcag ataatggctg ctgctccagt tgtgcgcata gctgactggc gctacgcatt
     2101 cccagactcg agcaactgct ttttagctga tgcgcggcac gctttatttt ctcgctgtcc
     2161 tggctggcgc gggcaatgtc gatttcatcg agaagcggca gggcatgttg tttaaataat
     2221 gccagccatt cgtggatctt ctccgtcccc attaactgag cgtcttcatt gagttgcgag
     2281 acatccagtg gtagatcgtt attggcttgc agttggagat agtgcgccag taactgaccg
     2341 agcacttcac gcggcaccgg tttagggata atcccgcgaa atagtgaact ggtacgctgg
     2401 cgcagcgttt cgtcaatgac atgggcgcta aagccaatca aaaccagcga cggatattgc
     2461 tgtgccagtt gccgggcaag ggtaatgccg tcgatatccg gcagatcaaa atccaccagt
     2521 gcggcagcaa acggttcgct attttgcagt gtctctaaag cctgcgcggc attgccaata
     2581 gcaacaacct gcgcaccact ggtgttcagc atctcaacgg taattcgctg ggttagcggg
     2641 ttatcttcaa ttaacagcaa acgtaaaccg tcaagacgca ccgcctgatt ggctgttttt
     2701 ggcacgggtg cagtggcaac acgtaacggc aagcgtaaac aaaaacagct tccaacctcc
     2761 ggcgtgctgg tggcgctcag ttcgccgccc atcgcctggg ccagacggct actgatagtc
     2821 agccccagcc cggtgccgcc gcgtttgccg cttacctgca caaatggctg gaagatttct
     2881 gccagtttcg cgggatcaat accgcagccg ctgtcttcca cttcgaccag ccattgctcg
     2941 ccatcagtgc gactacgcag gataatgtgc ccttcgtcag taaaacgcag ggcattgctc
     3001 aataggttgg taatgacctg acgaatacgt cgtggatcgc ccattaacgc gctcggtaca
     3061 tcatcggcaa ttgccgttgc caggcgaatc gggcgacctt tcacccgtcc gctcattaat
     3121 tgcagggtac tttccagcag cgggcgcggt tcaaagggct catcgctgac tgaaacattt
     3181 ttgccacctg cttcgatagc cgaataatcg agaatatcgt tgaggatggt cagcaacgat
     3241 tcgccagagt cggtaatcgc ctgcaaatca tcacgctggg cgttaagtgc ggggttatct
     3301 gccagcagtt gagcagtgcc gagaataccg tacagcggtg tgcggatctc atggctcatc
     3361 gccgtcagaa acgccgattt tgcctggctg gctttttctg cttccgcccg tgcctgtcgg
     3421 tgttctatca ccagttcctg caattcagct gtacgcgctt tgacctgcgc cgccagctgt
     3481 tcgcggtggc gattcagtgc atgaacactg ctgcgaaacg catccatcag ccgcccgatg
     3541 gtatccagct cccgtacgcc agcggtttcc gggaaagggg agtcaatatc accgtccagc
     3601 agccgttgca gcgcctgcgt ttgttcggca agtggacgcg tgaatgagcg ataaaccacg
     3661 cgccagagga tcataatcag tgcgcaaagt gaaaccatcc ccagcaataa caggctgtat
     3721 tgcccgcgtg cactggcttt ttccagatgt gccagtccat gctgattgtg cagctcaatg
     3781 gtgtcgacca gctgactgac ttcgctacta aactgcgcga actgggcgat attattttgg
     3841 gcgagagttt gcaggtgatt gctgatttca ctgtcctgct ggaacagcgc caacagatcg
     3901 ctatattggc taactgtagt taacgttgtt gcgacctgcg cacgaactcc cggatcttca
     3961 atgcgtattt gccgacgttg cagaattttc accgcattat tgagctgctt ttccagcgtt
     4021 ggtgcatttt tctggatctg ctccagcccc agattcatca ccatttgctg cacccgcaga
     4081 gcgctaaggc gcagttcatt catctggtta acatactcaa gatcgatatc aatcagccga
     4141 tcgagtgcac tttcagcagc ctgacgctga tcttgttcga tcaaatcgta aatcccggcc
     4201 tgggtcgctc cagcggaagt tgtcgcatta ttcgcctgac cttgctccag gcgtgcgatc
     4261 tcatcggcgg cagctatctg ctgactgagt tgccgttgtt gctggcgtag ttgcagacgc
     4321 cgccccacca gttccccttg ctgacgtaat gaacgggaga tctcctgctc ctgttgttca
     4381 atagcggtgg tatcaaaacc ttgttcccgt aacgcttgca gcaacgcatt aatcttcagg
     4441 ctttatgcgg tgagcattcg cccctgcgcc tgccacatct tttcgttatc ggcactggtc
     4501 aggttctgcg cggcgaaaag ttcccaggcg ctggcttcgc tcaactggcg cgccatattc
     4561 atggtaggaa tcaatgcctg agtattgtct ttttccacct ggctgataaa gcgcaggttg
     4621 taccatccca ccagggtact ggtcagggtt aacagcgcca tcagggcaaa gcccatccag
     4681 agtcttcggg tcagggttaa attcacggtc ggtgcactta aggtgaaaaa ggttgagtcg
     4741 caaagtggaa tgcatctagc ataaagcctt attattgatg aggctatcat gcgcgtactg
     4801 ctatttttac ttctttccct tttcatgttg tcggcatttt cggctgataa cctgttgcgc
     4861 tggcatgatg cgcagcattt cacggtgcaa gcctctatgc cgcttaaagc caaacgcgta
     4921 tggaaactgt gcgcgcttta tcccagcctg aaagattcat actggttatc gttgaactat
     4981 ggtatgcagg aggctgctcg ccgctacggt gtggatttaa aagtgctgga ggcaggcggc
     5041 tacagccagt tggctaccca gcaagcacaa atcgaccagt gtaaacagtg gggcgcagag
     5101 gccattttgc tcggtagtag cacgacgtca tttcccgacc tgcaaaagca ggtagcaaat
     5161 ctgccggtga tcgaactggt aaatgctctt gatgatcccc aggtgaaaag ccgcgttggt
     5221 gtaccctggt ttcagatggg ctatcaacct gggcgatatt tggtgcaatg ggcgcacggt
     5281 aaaccactga acgtgctgct gatgcccgga cccgataacg ccgggggcag taaggagatg
     5341 gtcgagggtt ttcgcgcagc cattgccgga agcccggtgc gtattgttga tattgcgctt
     5401 ggtgataacg atattgaaat ccagcgcaac ctgttgcagg agatgctgga acgccaccca
     5461 gaaatcgacg tcgttgccgg aacggccatt gcggcagagg cggcaatggg tgaagggcgt
     5521 aacctgaaaa cgccgcttac cgtggtgtcg ttttatcttt cacatcaggt gtatcgcggg
     5581 ctgaagcggg gaagagtgat tatggctgcc agcgatcaaa tggtctggca gggggaactg
     5641 gcagttgagc aggccatcag gcaattacag gggcaatcgg tgtctgataa tgtcagccca
     5701 ccgattttag ttctgacgcc gaaaaatgcc gaccgagaac atatccgccg ctcgctgtca
     5761 ccggggggat ttcgtccggt ctatttttat cagcacacat cagcggctaa gaaataacct
     5821 tcaccatgtt gcgtcaccag taaatccgcg ctgagtttat gacgtaaacg acgaattaac
     5881 acatcgacgg tgcgcaggtc agggttttcc acccgacgcg cagaaagcat acgcagcaga
     5941 cgctcacggc tgagaatttc gcccggatta gtcacaaatg ccaccaacat ttcatactct
     6001 gcgcgggtca gtttaatcgg ctcgccatcc cgctccagcg tatggcgcga cacattcagg
     6061 caataacccg caaagcgata gcagtagtcc tgagtgtgcg gttgagcttg tcgcgcgagg
     6121 tcgattcgcc agagcagatt tttcacccgt actaccagtt cgcgcagttc cagcggtttg
     6181 gtgacgtaat cgtctgcgcc catttccagc ccaacaatac ggtcaatccg atcgctgcgt
     6241 ccggtaacca gaataatccc caccgttgag cgttctcgca gggcgcgggt taacatcagg
     6301 ccattttcat cgggtaagtt gatatccagc agaattaaat ctaccggctg attctgcata
     6361 atttcccgta gcccggcacc gctcgccgta acggaaacgg tatacccctc ctgagtgaag
     6421 taggattgta atcgcgcctg ggtaaccggc ttatcttcaa caataacaat gtgatgtggc
     6481 atcagagggt tttactcatt ctgttcatat ctgttcatat ttgctcatta agatcgcttc
     6541 actaaaccat aattctacag gggttattat gcggaaactc tggaacgcgc tacgccgacc
     6601 cagtgctcgt tggtcggtac tggcgctggt cgcaattggg attgtgattg gcattgcgct
     6661 gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg
     6721 ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc
     6781 cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattacag gcatggtgaa
     6841 gcgcaaactg gaagcgagca acgacattta ccagaccttt attgcccact ccattgatac
     6901 acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa
     6961 agaaaacaac tcggcaacct gtcgctcctg ccataactac gatgcgatgg atcatgccag
     7021 gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat
     7081 cgactgtcat aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca
     7141 gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat
     7201 taagccaatt tatgcggcga aaggcgataa agaagcctct ggttctctgc tgcctgcttc
     7261 ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac
     7321 ggaaagcgcc ggacgtcagc gtgtactcac ccaattccca ggtaaacgca tctttgttgc
     7381 ctcgattcgt ggtgatgtgc agcagcaggt gaaaacgctg gagaaaacca ccgttgccga
     7441 caccaatacc gagtggagca agttgcaggc caccgcgtgg atgaagaaag gcgacatggt
     7501 gaacgatatc aaaccgatct gggcctatgc ggattcgttg tacaacggca cctgtaacga
     7561 gtgccacggc gcaccggaaa tcgcccactt tgacgctaac ggttggatcg gcacgctcaa
     7621 cggcatgatt ggctttacca gtctcgataa acgtgaagaa cgcactttgt tgaaatatct
     7681 gcaaatgaat gcgtctgaca ccgcaggtaa ggctcacggc gataagaagg aagaaaaata
     7741 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc
     7801 ttaaccgtcg ccgggatgct ggggccgtca ttgttaacgc cgcgccgtgc aactgcggcg
     7861 caagcggcga ctgaggctgt catctcgaaa gagggcattc ttaccgggtc gcactggggg
     7921 gctatccgcg cgatggtgaa ggatggtcgc tttgtggcgg caaaaccgtt cgaactggat
     7981 aaatatccgt cgaaaatgat cgccggattg ccggatcacg tacacaacgc ggcacgtatt
     8041 cgttatccga tggtacgcgt ggactggctg cgtaagcgcc atctgagcga cacctcccag
     8101 cgcggtgata accgttttgt gcgcgtgagc tgggatgaag ccctcgacat gttctatgaa
     8161 gaactggaac gcgtacagaa aactcacggg ccgagtgcct tgctgaccgc cagtggttgg
     8221 caatcgacgg ggatgttcca taacgcttcg gggatgctgg cgaaagctat tgccttgcat
     8281 ggtaatagcg ttggtacggg cggagattac tctaccggtg ctgcgcaggt gatcctgccg
     8341 cgcgtagtcg gttcaatgga agtgtatgaa cagcaaacct cctggccgct ggtattgcag
     8401 aacagcaaaa ccattgtgct gtggggctcc gatttgctga aaaaccagca agcgaactgg
     8461 tggtgcccgg atcacgatgt ttatgaatat tacgagcagc taaaagcgaa agtcgccgcc
     8521 ggtgaagttg aggtcatcag catcgatccg gttgtcacat ccacccatga gtatctgggg
     8581 cgcgagcatg tgaagcacat tgcggttaac ccgcaaactg acgtgccgct gcaactggcg
     8641 ctggcacata cgctgtacag tgaaaacctg tacgacaaaa acttcctcgc taactactgt
     8701 gtgggttttg agcagttcct gccgtatctg ctgggtgaga aagacggtca gccgaaagat
     8761 gccgcatggg ctgaaaaact gaccggcatt gatgccgaaa ccattcgtgg gctggcgcgg
     8821 cagatggcgg cgaacagaac gcaaattatt gctggctggt gcgtgcagcg tatgcagcac
     8881 ggtgaacagt gggcgtggat gattgtggtt ctggcggcga tgctggggca aattggcctg
     8941 ccaggtggtg gttttggctg gcactacaac ggcgcaggca cgccggggcg taaaggcgtt
     9001 attctgagtg gtttctccgg ctctacgtcg attccgcctg ttcacgacaa cagtgactac
     9061 aaaggctaca gcagcactat tccgattgcc cgttttatcg atgcgatcct cgaaccgggg
     9121 aaagtgatca actggaacgg taaatcggta aaactgccgc cgctgaaaat gtgtattttt
     9181 gccggaacta acccattcca tcgccatcag cagatcaacc gcattattga aggctggcgc
     9241 aagctggaaa cggttatcgc catagataac cagtggacct caacctgccg ctttgccgat
     9301 atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc tcgaccagta cggcaaccac
     9361 tccaaccgtg gcattatcgc catgaaacag gtggtgccgc cgtagttcga ggcgcgcaac
     9421 gactttgata ttttccgcga gctgtgccgt cgctttaatc gtgaagaagc ctttaccgaa
     9481 gggctggacg aaatgggctg gctgaaacgc atctggcagg aaggtgtaca acaaggcaaa
     9541 ggacgcggcg ttcatctgcc agcgtttgat gacttctgga ataacaaaga gtacgtcgag
     9601 tttgaccatc cgcagatgtt tgttcgccac caggcattcc gcgaagatcc ggatctcgaa
     9661 ccgctgggca cgccgagtgg cctgattgag atctactcga aaaccatcgc cgatatgaac
     9721 tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa tcgaacgctc ccacggtggg
     9781 cccggctcgc agaagtatcc gttgcatctg caatctgtgc atccggattt ccgacttcac
     9841 tcgcagctat gtgagtcgga aacgctgcgt cagcaatata cggtagcggg taaagagcca
     9901 gtgttcatta acccgcagga tgccagcgcg cgcggtattc gtaacggtga tgtggtacgc
     9961 gtctttaacg ctcgcggtca ggtgttggca ggagcagtag tttctgaccg ctatgcaccc
    10021 ggcgtggcgc gaattcacga aggggcatgg tacgatccag ataaaggcgg cgagcctggt
    10081 gcgctgtgca aatacggtaa ccccaacgtg ctgaccatcg acatcgggac ttcgcagctg
    10141 gcgcaggcga ccagtgcgca cactacgctg gtggaaattg agaagtacaa tggaacagtg
    10201 gagcaggtga cggcgtttaa cggccccgtg gagatggtgg cgcagtgcga atatgttccc
    10261 gcgtcgcagg tgaaatcatg accacgctga cagcacaaca gattgcctgt gtttacgcct
    10321 ggctggcgca gttgttctcc cgtgaactgg acgatgaaca actgacgcaa atcgccagtg
    10381 cgcagatggc tgaatggttt tcgttgctga aaagcgaacc gccgctcact gcggcggtga
    10441 atgatctgga aaacagtatt gccac
//
LOCUS       DAJTRD010000120        10333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTRD010000120 DAJTRD010000000
VERSION     DAJTRD010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.120"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..441
                     /locus_tag="OO315_003505"
                     /pseudo
     CDS             1..441
                     /locus_tag="OO315_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            524..781
                     /locus_tag="OO315_003506"
     CDS             524..781
                     /locus_tag="OO315_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892278.1"
                     /translation="MTLVNDTGFDPVFSGSIAESWRQQPCTPSYCCDWEAATMLRAFP
                     LAKKGEGRARLPSLYASFGKLGETPTHEDIIDNNRSINWPV"
     gene            complement(1339..2106)
                     /gene="fecE"
                     /locus_tag="OO315_003507"
     CDS             complement(1339..2106)
                     /gene="fecE"
                     /locus_tag="OO315_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HCR5892279.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(2107..3063)
                     /gene="fecD"
                     /locus_tag="OO315_003508"
     CDS             complement(2107..3063)
                     /gene="fecD"
                     /locus_tag="OO315_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HCR5892280.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(3060..4058)
                     /gene="fecC"
                     /locus_tag="OO315_003509"
     CDS             complement(3060..4058)
                     /gene="fecC"
                     /locus_tag="OO315_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HCR5892281.1"
                     /translation="MTAIKHPMLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(4055..4957)
                     /gene="fecB"
                     /locus_tag="OO315_003510"
     CDS             complement(4055..4957)
                     /gene="fecB"
                     /locus_tag="OO315_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HCR5892282.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAAMVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGSSMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(5002..7326)
                     /gene="fecA"
                     /locus_tag="OO315_003511"
     CDS             complement(5002..7326)
                     /gene="fecA"
                     /locus_tag="OO315_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HCR5892283.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            complement(7413..8366)
                     /gene="fecR"
                     /locus_tag="OO315_003512"
     CDS             complement(7413..8366)
                     /gene="fecR"
                     /locus_tag="OO315_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HCR5892284.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(8363..8884)
                     /gene="fecI"
                     /locus_tag="OO315_003513"
     CDS             complement(8363..8884)
                     /gene="fecI"
                     /locus_tag="OO315_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HCR5892285.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            9182..9364
                     /locus_tag="OO315_003514"
                     /pseudo
     CDS             9182..9364
                     /locus_tag="OO315_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012767718.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9358..9462
                     /locus_tag="OO315_003515"
                     /pseudo
     CDS             9358..9462
                     /locus_tag="OO315_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            9802..10059
                     /locus_tag="OO315_003516"
     CDS             9802..10059
                     /locus_tag="OO315_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm development YmgB/AriR family protein"
                     /protein_id="HCR5892286.1"
                     /translation="MHSDYYNMVFGEKLANILYEANSQFFYERNVIEEAVNALFCERE
                     IINNKNIIKKLMFFLSDVNHTKKDVVQSALNIIIDITSGDI"
BASE COUNT         2409 a         2747 c         2833 g         2344 t
ORIGIN      
        1 tctcgacctg ttcgcaagaa aaccagtggg ctgggccatc tcgttctcgc cggacagcag
       61 gcttaccatg aaagcactgg aaatggcatg ggaaacccgt ggtaagcccg tcggggtgat
      121 gttccaagcg atcaaggcag tcattatacg agcaggcagt tccggcagtt actgtggcga
      181 taccagatca gacagagtat gagccggcgt ggaaactgct gggataacag cccaatggag
      241 cgcttcttca ggagtctgaa gaactaatgg gtgccagcga cgggctatgt aagcttcagc
      301 gatgcagctc acgcaataac ggactatatc gttggatatt acagcgcact aagaccgcac
      361 gaatataatg gtgggttacc accaaacgaa tcagaaaacc gatactggaa aaactctaac
      421 gcggaggcca gttttagttg accacaacag actacctgaa gggagccgcg gtcgcctagc
      481 agttgcagta gcaggagatc atccagccgc agtacaggtc acgatgactc tggttaatga
      541 taccggcttt gaccccgtat tttccggctc tatcgctgaa tcatggcgtc agcagccgtg
      601 cacaccatcc tattgttgtg actgggaggc tgccaccatg cttcgcgctt tccctctggc
      661 gaaaaaggga gaaggacggg cccgtctgcc ttcactttat gccagcttcg gtaagctggg
      721 tgagacaccg actcatgaag atatcattga taacaatcga tccatcaact ggcctgtata
      781 acgtggctgc cggtgattaa gaaagctgca cctacctaag tagtagcaaa cgcacacttt
      841 ttagaaaaat cgatggtcag aaactggatt agcaattccg ttccagggtt gcttttgatt
      901 tacgttggcg tctgatcatt gatttatcct caaaagccca acctcattgg taatgaacca
      961 gctccgtgaa tgtccgctct ggcacagagc gaaatttttt gatctccccc cctgaaatct
     1021 aaacttagtc atgtcacgtt tttgggtttc taaaatttta acttcgcgtt tttcgttgcc
     1081 gtaagggtta tacagaaatg tccgttaagc agagttcaaa attgattgcc gtgatcacga
     1141 ctggtttgaa agccgcgccc aagcctgtac agctctggtt tgcgttgatt atgaacctgt
     1201 cagcctaaag caagcggatg gacgatgagt attggtaatc tttcagagtc cggaaaagtt
     1261 cagccccagt ctgaacaggc ttgctggcgc cagtccagtt tcattcagtc gtggtttggt
     1321 tcttacggcc tgtgcaatct acctcattag gcacatcggc ctgccagata ccggctcggg
     1381 gtgtatttcc gcttccacgc tgaatactgt tctcagcaat cctggggtca tcacctcttc
     1441 tggtgtgcct tgcgccataa catgtccgtt tgccattacc accagttgat cgcagtaccg
     1501 gctagcctga ttaaggtcgt gcagcacagc gaccaccgtt ttcccctgag tccggagttc
     1561 gcccatcaac cgcatcaggt ccacctggtg attgatatca agataggtgg ttggctcatc
     1621 aagtaataca acgggcgtat tctgggccag gaccatcgcc agaaatgcgc gctggcgctg
     1681 accgccggaa agctcggtta accgacgaac ggcaagatga ttgatccggg tctggttcat
     1741 ggcgacatta actcgtgcat tgtcttcagc ggagagacgc ccccagagtg acagccaggg
     1801 attacgacca tacgaaacca gctcctggac tgtgatcccc tctggcgtta aatggtgctg
     1861 aggcagcagc gaaagcctgc gggccaactg gcgtgatgag agcatattta tgggattatc
     1921 gccgagaaat acggtgccag actgcggcat taaaagccgc gaaaaacagt ttaacagcgt
     1981 cgatttcccg caaccgttag ggccgatcag ggcggtgatc ttccccgttg gcagtgagag
     2041 tgaaacgtcg ttaagtacct tgtctgtccc gtaactgacc gtcagatttt cagttcgtaa
     2101 agtcatttat cgcattctca caagcaacca gacaaaccac ggcgcaccga taatggcggt
     2161 cagcacgcca accgggagct ccagtggggg atgaataatt ctcgccagca gatcggcaac
     2221 caccaacagc aacgcacctg tcagggctga aacaggcagc agtctgcggt gacgtccacc
     2281 ggtgatgcta cgcatcatat gcggcaccac gagaccaata aagctaatcg ggccgcaggc
     2341 ggccacgccg gtagatgtca tggcgacagc tagtaacaaa gcccagaatc gggtatgggg
     2401 caccgacaca ccgagcgtgg tggcgcgcgc atcgccgagt gcaaggaggt cgagatcgcg
     2461 gcaaaaactc aggctcagcg gcagaaataa aatcatcagc gggatggcaa tcttcacaaa
     2521 gctccagtca cggccccata agctgccggt cagccacagc agggcgttgt tcacatcctg
     2581 cgggcgcgag agcatcagat aatccgtcag gctggcccag catgcagaaa gcgccacgcc
     2641 ggtgagcgcc agcttcatcg gctggtgggt ctttgccagc atcttcagta atatcaaccc
     2701 cgccatgccg cccgcaaagg ccagcagcgg cagcaccatc acgggcagtg acggcataag
     2761 aagtagagcc cccacagagg ccaggctggc ggcatggtta acgccgagaa tatccggtga
     2821 tgccagaggg ttgcgcacaa tcccctgtat cagcacgccc gccacggcga gggctgcacc
     2881 gacaaacagt gccagcagca agcgcggcag tcggtactcc atcaatacat aataatgctc
     2941 gcgtccggcc tgccagtcgg tcagcagcgc gcgccacggc acggggatca ctcccatatg
     3001 gagtgataac agcgcacagc ccgccagggc aagggtgatg aaaataacca gcgcaatttt
     3061 catcctcgcc tcctcacaag ccagacaaag caagggctgc caatcagcgc cagcactgcg
     3121 cctgcgggca gatctccggg gaaggccagc gcgcgtgcga gtacatctgc cagcagcatc
     3181 agcgtggccc ccagcagcat gctcactggc agtacgttgc gctgatcgaa gcctgcccag
     3241 aagcgcgcca gatgtggcac cagcagaccg ataaacgcca ccggacctgc cacgctgacg
     3301 cacgcaccaa ccagaagcag cactaacata ttgatgacca aacgtagcct cgtcaggttc
     3361 actcccagcg tatgggcggt gctgtcgctg aggttgagca ggttcagttg attcgccagc
     3421 agtaacacga cagggactgc agtgaccacc accggcaaga gctgccagac atcctgccag
     3481 cgggcgtggg acactccgcc tgccagccag taaaagatgc cgtaagcatg atcttcggcc
     3541 agcagcaggg tgatgcgggt aaggcccata caaaaggccg acagcgcgat acccgcgagg
     3601 atcagtttgt ttctgtcatg ggtatgacga aatccgcctc ctgcggtcat gaccagcagc
     3661 cagctcacgc cgcccccgca tgccgcgatg aacgacagag aatagcctgc aatcggcgtc
     3721 ggactcagcg cgctggtaag cgccatagcc agcgccgcgc cgctgttaat gccgagcagt
     3781 gaaggagagg ccattgggtt gtgggtcagg gtttgcagca gcgtgcccgc gagcgccagg
     3841 cttgcgccga tcagaacggc gaccaggctt cgtggcaaac gaaggttttg caccagcgct
     3901 tctggtagcg ttggcgtgtg tccaggcagc agggcgcggg ttgcatctgc tccggaaaca
     3961 ggaatggccg agtagcaaaa cagactcagc cagaaaataa taataagtgc tgcaacggga
     4021 agcccccaca gcagcatcgg gtgttttatc gcggtcattt cacaacggta agcggctgat
     4081 ggtggaagat ttttaccgtg tcagcggcaa tacgctctgc agcaaaaata ccgcgcatcc
     4141 gcgcccaggt gttactgtcg accgaagcaa cctgctgctt ctgcgcggcg gttaacatct
     4201 gccagagcgg atcttgttgc cagcgtttaa caatgctctc ttcgcgatag tgggcaacca
     4261 gcagccaggc aggattgacc gccagcagtt gctccaggcc gatggacggc atggacgaac
     4321 ccgccatcgc agcgggaacg ttcagcccca gagaggccag cacgctgccg gtccaggtct
     4381 cctgagtatg caggttgaat tgctgttcgc gtgatgtgcc aaaggccacg cgtgtccctt
     4441 tgggaagctg gctggcccac tgcgccatcc tctctttatg ttgttccaga cgtgcctgca
     4501 tctctcgctt tttacccacc atttcgccga tgatagccgc agattgcaaa ttttcagcgt
     4561 aggtttcgtt gcgggactta agcagcagta ccggcgcgat ttgctgcaag gcgatgtaaa
     4621 cccccgcatg gcgactgctg tcggcaatga tcaggtctgg tttcagagcg gcaatggctt
     4681 ccaggctcgg ctgcgcgcgc gttccgacgg actgccacgg tttcaggtgc gcacgcactt
     4741 cgggcaggat gcgttttgca tcgttatcgt cggcaatacc gatcgggctg acgtccacgg
     4801 cggccagcgc atcggcgaac gagagttcca gcaccacaat ccgttgtggc gttttttcga
     4861 gtgtaaacgt gccgtgttcg tcctgaacca tggcggcaaa ggcgtggctg atcaccagca
     4921 gcaggcctgc aaaaagaaaa cggataaatg ccaacataat cacattccag ctaaaagccc
     4981 ggcaagccgg gcgttaacac atcagaactt caacgacccc tgcatataca gcgtgcgcgg
     5041 ctggcctgca tagatgcctt tgttgttgtc gtcataagag cggatgaagt agtcctggtc
     5101 gaagatgttt ttcacaccga atgccaggtt cagatctgcc atctgcgggc caaagtcata
     5161 cgccacgcgt gcaccccaga gcatgaagcc gggaatgcgg ccggtactgc cgtcggcgct
     5221 ctctttcacc gtattggcgt tatccgcaaa ctggctggac tggaaatcgc tgttcagatt
     5281 gaacgtccag tttcctggct tgtagtccac gcccagcgtg cctttatgtt tcggggagaa
     5341 tggtaccaga ttgccgtagg tgtcgccttt ctcgcggatt tccgcgttca catacgcata
     5401 gctggcgtag atggaaacgt tatcaagcgt tggcgttagc gtacccagat cgtaacgtgc
     5461 ctgcgtttcc agcccggtat ggcgcgtttt gccacgtgca gtgacggtgt cgttggtctg
     5521 gttggagtcg tactgattgt taaagttaat caggaacagc cccatttccg ccgtcagcgc
     5581 gccgtcgtcg tagcgggtac cgagttccca ggttcgcgct ttttccggtt ccacattgcc
     5641 gctttgcaca gccttgccaa tctggctgta ctgtacggtg ccgaacgagc cttcagtgtt
     5701 tgcataaaga ttccagctgt cagtcaggtg atagagcacg ttcaacgccg gaagcggtgc
     5761 gttatagctc acttcttcgt gcgtgcctgt gatggcgttg ttctggtatg actcgatatg
     5821 ttcgaaacgc atacccggcg tgatggtcca gttgccgatg tcgattttgt catccagata
     5881 ccaggcgtgc gcctcggtgc cggaacgcgt atcgcggtcg taagggcttg agccggacgg
     5941 caactgcccg ctgctggtgg cggtgtagta acgcatttca tgcgttgatt cattcacata
     6001 gcgatagccc acgcccactt cgtgcgcgga agggccgatc ataaagatct ggctgtagcg
     6061 tggctcaata ccgcgcaccc agtagttacg cggcgagagg gtgatgcgtt tgccttgctc
     6121 caggtagccg ctgcgcaggg tttgggtgta gaacccctga atgttgaatt tatgctggct
     6181 gtctggctgg aactggtagc ccaggctcgc cagcttgcga cgaccccaga agcggtcata
     6241 cgggcgggtg gattgccagc gatcggcgtc gtaatccgcg cgagacaggc caccgggcat
     6301 gtcggcttca ccgtcgtaat attgcagcag gctgttgaag gtgtgcacct catccggcgc
     6361 atatttgctt ttcagcatca ggtcgtcgat gcgggtggcg ctgtgctcgc gccagtcact
     6421 gccgcgcgtg ccggagtaga gcagcgcggt gccaaaaccg ttgtccgctg tgccgcccac
     6481 catcaggttg tgcgtctctt tcgggttgtt ttgtgaagag gttgggctga gctgaccttc
     6541 cacgcccgcc tcgataccaa agtcctgcgg aatggcacgg gtaacaaagt tcaccacgcc
     6601 gcccacgctc tgcggtccgt aacgcaccgc accaccaccg cgtaccacgt caatggcatc
     6661 catgttgccg agcgaaacgg gagccagtga aagctgcggc tgaccgtaag gggcgaaggg
     6721 gacggggatg ccgtccatca ggacggtcga gcggctggcg aggcgcgggt tcaggccccg
     6781 gatgccaaag ttcatcgcca ggtcgtggct gccggtgccg ttgttttccg gcgcgctgac
     6841 gccagggatg cggttaagta cctcacgcat ggtggttgcg ccggttttgg cgaaatcctc
     6901 acggcggatc acgtcacgcg cgccagcatg ttcaaatacg tcgttttcac gcgcatcacc
     6961 cagccagtcg ccgaccacgg tcagggcatc ttcttttggt gcgggcgcgg gctccagcgt
     7021 ccagctgtta tttcccagcg gttttacctg cagtccgctg ccgtccagca gttgttgcag
     7081 gccgctctcg acgtcgtaat cgccgtgcag gccgttgctc tgcttgccgc gcgtcaggct
     7141 ggcgtcaacc gagagggtaa atccgctgtg tgcggcatac tgattgagcg ctttatcgag
     7201 cgatcccggt gcgatattaa cctgtgcagc aaaagcggaa aacgagagac cggccagcgg
     7261 cagcaggctc aggcgaatgg cgttaaccaa aggtgttgtt ttacgaaaaa cgcgtaacgg
     7321 cgtcatacct tccccatcat catttttgtt gtgttcagct atgagtcgaa cgagaagggt
     7381 aaaaaggaca atcgaaataa gaattatttt ccttacagtg gtgaaatgtt tatccagtac
     7441 cgcgtaatag actgaatttt aacgggaagc gtttgcgcga taacgttcag gatcgcatcg
     7501 gtatttttca gcgggaacgt cccgctcagg cgcagcccgg caacggcggg atcgcagcgc
     7561 agcacgccgt tgcggtaacg ggttagcgtg gctatcacct cacccagcgg tttatcgctg
     7621 aagctcagga tgtccttcgt ccagcttgta ctctcgtcat ccagcggttt cactgcgcca
     7681 aactcagagg cgctgaactg caggctttca ccagcgttca cgatgcgttt ttgcgcgggg
     7741 gcactggcga gaagcacttc cacagcgtgc tgctgaacgt caagctgcgt gaaattatcc
     7801 tgctggcgga cggtaaattc tgtccctaaa gcagtgagct ggccctgacg ggtcaggaca
     7861 cggaaggggc gttgcagggc atctttcgcg gtagtaatgg cgatttcacc gtaccagagc
     7921 cggacggtgc gctgatgcgc atcaaaacgc acatccgccg cgctttgggt attcagcgtg
     7981 agcagggagc catcttccag ttgctgacgg ctgactgtgc ctttggcggt gcggtaatct
     8041 gcccgcagac cttcgccggt ttccgactgc cagagctgcc agcctccgcc agcgccgagc
     8101 aacagcagta atcctttcat cacgtgacgg cgggtgaggc gggtatcgtg caacgcccgg
     8161 ctggcaacgt cgccaggcac accgccaagc tggttgcgca ggttttcaac ctgctgccag
     8221 gcccactggt tatcctgatc ctgttcatac cactgttgcc agcgcgcttc ctgttgtggg
     8281 ctgacgcgct cgccgcttag cacggcatac cagtgggaag ctgaacgcag cgcctgacgg
     8341 cgggaatcgg ttaacaaagg attcataacc catactccag acggaacagc aggcagtgct
     8401 cgacggcttt cgccacgtat tttttcacgg agctgatgga aacaccgagt ttgtgcgcaa
     8461 tctcgctgta tgtcagacca tccagttgcg aaagcagaaa cgcttcacgt gttttgccgt
     8521 ttagcccgtc cagcatgctg tcgaggagtt gtagggtctc gagttggctt tcgcgttcct
     8581 caggtgaagg cgctcccccc tccggcataa gcgccagcat ctccagatac gctttttcca
     8641 gcgcgtttcg gcgaaacagg tccaccatca cgcgtttggc gatagtgcag aggaaggagc
     8701 gaggatcgcg gatcgtcgag agcgtttcgc tgaccattac ccgcaaaaaa gtgtcctggg
     8761 caatgtcatc tgcatcaaaa gcagactgga gtttgcgcgt cagccagctt ttcaaccagc
     8821 cgtgatgtgt gccataaagc gactcgaacg ttaaggaagc tgtggtagtg gcgcggtcag
     8881 acatgcggag tgcatcaaaa gttaattatc acgtagtcat attaatatga gaatggttat
     8941 cattacaatt ggaaataaaa ttgtttccaa tagacatttt taacatgttg tttttctaag
     9001 tgttataagg taggtataaa atgggatgga gcctctgctt ctgacatgtg tcggtcagaa
     9061 tgactcatga tgtggtctgc tattattgac atcctcactg ccctaaagga tggggatttc
     9121 ggtaatgatc ttacccagca atagtggaca cgcggctaag tgagtaaact ctcagtcaga
     9181 ggtgactcac aagacaaaac agtttcaacc agtaaaaaaa cgtaaacacc attcgcatga
     9241 atttcgtagt taagatctga agcttgcaga atgcatcggt gttactgccg gagtccgtga
     9301 acttagtctg catgaatcac cacagcttta catctgtcgc agtaaacagc aaaatcagca
     9361 aacttactta attatatcga gtgcgattac aatcgctggc gatggtacag ttggtgtggc
     9421 ggtctcagtc cggaacaatt tgaaaatcat aatctcactt aaaacggcat ccattttaca
     9481 aaagatggcc gtctgtgtat ttgtaatgaa taccttctac atgtttagag tgcataatcc
     9541 acgacaaggc gatatcctaa aaattagcta gcaggcaact ttgatggcat agataatgtt
     9601 tttagtaaaa aagagttgac tgaatataat acagcctctt cctacataag aaggaatgtg
     9661 ctcacaaaaa aaaaccatta gtctgcttcc tgttcttaca ataatcagta ttatgtattt
     9721 tatttatata gcaaaatatg cttgttacac ctgtcataat taggcatggt aaaattattg
     9781 actgaaccct gggggagaaa gatgcacagt gattattata atatggtttt tggtgaaaaa
     9841 ttggccaata ttttatatga agctaactct caattttttt acgagagaaa tgtcatagaa
     9901 gaggcagtca acgctttatt ctgtgagcgt gaaataataa acaacaagaa cataataaag
     9961 aagctgatgt tctttttgtc agatgttaat cacaccaaga aagatgttgt tcagagcgca
    10021 cttaatatta tcattgacat tacatcaggt gatatttgag attttacata aaagataatc
    10081 tctcaaggag tcttatctga aaacgaaata tatagtccct ctcgttttgt aatatcgatt
    10141 ggctttgttg aatcggttac gtaccccgga gccggtcata ttttcaggta atacacacgc
    10201 tttcagacat ttcggctttc gtcatttttg tatcatgggc atagcttctg ctacctgacc
    10261 atcataatca cgcagcttca gtgaagcagt gaccacctgt ttaacccggt gtatcgttat
    10321 gatcttatcc agc
//
LOCUS       DAJTRD010000121         9937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTRD010000121 DAJTRD010000000
VERSION     DAJTRD010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.121"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="OO315_003517"
     CDS             complement(<1..1023)
                     /gene="bglB"
                     /locus_tag="OO315_003517"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5892287.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="OO315_003518"
     CDS             complement(1042..2919)
                     /gene="bglF"
                     /locus_tag="OO315_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5892288.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="OO315_003519"
     CDS             complement(3053..3874)
                     /gene="bglG"
                     /locus_tag="OO315_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5892289.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="OO315_003520"
     CDS             complement(4161..4886)
                     /gene="phoU"
                     /locus_tag="OO315_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5892290.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="OO315_003521"
     CDS             complement(4901..5674)
                     /gene="pstB"
                     /locus_tag="OO315_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5892291.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="OO315_003522"
     CDS             complement(5766..6656)
                     /gene="pstA"
                     /locus_tag="OO315_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5892292.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="OO315_003523"
     CDS             complement(6656..7615)
                     /gene="pstC"
                     /locus_tag="OO315_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5892293.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="OO315_003524"
     CDS             complement(7702..8742)
                     /gene="pstS"
                     /locus_tag="OO315_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5892294.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8990..>9937)
                     /locus_tag="OO315_003525"
     CDS             complement(8990..>9937)
                     /locus_tag="OO315_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5892295.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2563 a         2586 c         2295 g         2493 t
ORIGIN      
        1 tccaaccgga tcaatttgcc acccccactc tgaacttttc agatgcggat tggggatcat
       61 attcagtatg ttgccctgcg catttttatt aatgctttcg tcgtgggaaa cacaaccagt
      121 catgtaataa ctaaaagaga tgaaatctac agtatgtttt aaatcttctg cgtcactttc
      181 agtcatctca atggtgatat tgtggtcgcg gaagaaacgc tgcatatagc cgggatattg
      241 gccacgcgcc tgaacatcac caaagaacat ccagcgccgg ttctcttcca tggcctgcaa
      301 catatcctgt ggctggcagg tgagggggta aaccagccca ccgagaagca tattgccgat
      361 tttcgcttcg gggatcaggc tatgataggc tttaactgcc cgcgcactgg caaccagttg
      421 atgatggatc gcctgataaa cttccgcctc gccactctct tctgccagcc ccacgcccgt
      481 gaatggcgcg tgtaacgaca tattgatttc attaaacgtc agccataacg ctactttatg
      541 ttggtagcgg gtaaagaccg tgcgggcgta atgctcgaag tgatcgatga ccgcccgatt
      601 agcccaaccg ccgtagtttt tcaccagccc atatagcatt tcgtaatggg ataacgttac
      661 cagcggcttg atccccgcct gcgccatttc atcaaacagc cgatcgtaaa acgctaaccc
      721 cgcttcattc ggttcggctt cgtcgccctg agggaaaatt cgcgcccagg caatggaaat
      781 acgcagacag gtgaagccca tctcggcaaa taacgcgata tcttccgggt aacggtaata
      841 aaaatcgatg gcgacatctt tgatattctc tttccccagg atgcgcggtt ccatttttcc
      901 cattacgcca tgaggctgta aatctgaggt cgtgatccct ttgccatctt ccagccaggc
      961 accttccacc tgattggcag ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt
     1021 cataattaac tccttttatc gttagcgaat gatggataac agcggttcac ctgcgcttat
     1081 ctgcgccgtg ccgtggggta atacgtctgt aaaatcatcg ctattactga ttaataccgg
     1141 cgtcgtcaga tcaaatccgg cctcgcgaat agcagggata tcaaaagaaa tcagccgatc
     1201 gcctgtattg accttgtcac ccacgttgac gtgagcggaa aagaatttgc cgtccagttt
     1261 tacggtgtcg ataccgacat gaatcaggat ctccacacca tcatctgact caatgccaat
     1321 ggcgtgtaat gtggcgaaca acgaagcaat tcgacccgca accggagaac gcacttcacc
     1381 aaccgagggc agaatggcga tacctttacc caacaggcca ctggcaaacg tggtatcagc
     1441 gacgtgaatg agcggcacaa tctctcccgt catcggtgaa cagataccgc cctgctcagg
     1501 tggtgtaata acctctggtg ttttctcttg cggggcaccc tgcgctggct gacgtttact
     1561 ggtgatgaaa tgaagcatca acgtaccaac aaatgcgcaa cctatggcaa tgacaccgcc
     1621 aataacgctg gcccagacgg tgaaatcaat ccccgttgac gggatggtct gcatgaaggt
     1681 gaaaatactt ggcaaaccaa aggagtaaac tttcgtttgc gcgtagccaa tgatggtggc
     1741 ccccaatgct ccactgatac aggcgataac aaaggggtac ttacgcggca ggttgacgcc
     1801 atataccgct ggttcggtga taccaaacag acccgtcaac gccgctgatc ccgccaccac
     1861 ttttttctgc gcatcgcgtt cgcagaggaa gacgccgagc gccgccccga cctgcgccat
     1921 aatggcgggc attaacagcg ggatcatggt gtcgtagccc agcacggtga agttattgat
     1981 acacagcggc accaggcccc tgtgcagtcc gaacatgacg aagatttgcc agaagccgcc
     2041 cattaccgcg cccgcaaatg caggaaccgc ctgataaagc cagagataac cggcggcaat
     2101 catttcgctt atccaggttg atagctgccc caccagcaga aaggtgacgg gtgtgataac
     2161 catcagacat agcaatggtg tgaagaaatt tttgattgcc gacggtaacc acgcattaag
     2221 tcggcgttcc agaatgctgc acaaccaggc agaaaaaata atgggaataa ccgatgacga
     2281 gtaattcaac aatgtgaccg gaatacccag gaaatccagc cccggcgcat ccgctttttg
     2341 cccgttctcg aaggcagtca gaattaatgg atgcactaac gctccaccaa tcaccatggc
     2401 agtaaatgga ttaccgccga agcgtttgcc cgcggtgtat cccaggatta tcgggaagaa
     2461 cccaaacaag gcatcactgg cgctgaataa aattaaataa gtaccacttt gttcggtcgt
     2521 ccactgaaaa gtgagcgcca gagccagcat acctttcagg atcccggttg ccgccatcag
     2581 accgatcaga ggcgtaaaaa tacctgaaat aacataaaca aagcggttta gcagattacc
     2641 tttctcatca ttttccggtg cctgttgcgc tttttcgcca aggcctgcca cgctgttaac
     2701 cgccaggaag acatcggcca catggttacc tatgaccacc tgaaactgac caccgctttc
     2761 caccaccata ataataccgg gggtcttttt cagtacctca gcttgcgctt tgctttcatc
     2821 ctttaactta aaacgtaatc gcgttgcgca atgcatcaga ctcacaatgt tatctgcgcc
     2881 cccgactcct gcgactattt ttctggctaa ctccgtcata acttgccctc taccgctttg
     2941 cggcaaaact ccaaaaaaaa acctgaaaaa aacggcctga cgtgaatcaa gcaatttttt
     3001 tcaggttttg cccgcttagt gcggtaacaa tcctttactc agtaataata tttcagtgtt
     3061 ctttgcgcac gcgctctata tttatggcta aaaacataat ctctgcgggt gaaattttac
     3121 gttgatactg caaaccaata aaaatggcga tccgttccgc acattgccat gcttgcgggt
     3181 aattttgttt tactgcttgt tgtaatgatt catcactatc gttaattgaa gcatgttcaa
     3241 gaatacgcca ggataaaaac ttcagatgtg taaccagtcg ctgataactc aagctttctt
     3301 cctggtaatt aaggctgaac tgaaatttta ttaattgcag catttcgcgc attaactgcg
     3361 tgacacctgc aacatcctcc atatttccgc tcatttgggc actgaccaga tgcatggcaa
     3421 taaagcccac ttcatctttc ggtaactgca cgcccaaccg tttatcaata atggttaacg
     3481 cttcttcccc tagctggaac tctttcgggt aaagccgctg gatatcccac agcaacacgt
     3541 tttgctgaaa gcgtttaatc gcaaactggc aatggtcagt tagcgagata taaatactgt
     3601 cctgtaattt tcccaagcgc tcctgcgcta aagagataag acgatcacag gttgccatca
     3661 cctcaagagg aatatgactt aagagttcgc ttaatcgccc gttcagttca tgactgctca
     3721 aggcatactc tttttctatt ccacttgagt taattctttc gccagcgcgt ttttgaaagc
     3781 caattccgcg ccccatgacg actttttccc gctgttgatc atcaataacc accacaacat
     3841 tattgttgag aattttggtg atttgcatgt tcatagcaag gacctttttt ataaacaaaa
     3901 aaaaaccgac ttcaccagta ttctctggtt atgtcaggtt ttgcctgcga atgcagtaac
     3961 aatccagtca tttattaatg gtttttataa cgaacatcca ggttcggaaa ttaatttaat
     4021 tgcgtgcaga gaaaatagca atgcgctatt gataaaaata tgaccatgct cgcagttatt
     4081 aactttgtgt aattttagga atttataaag ttatatataa caaatcccaa taattaagtt
     4141 attgggattt gtctggtgaa ttatttgtcg ctatcttttc ccgccagcag tttatccagc
     4201 tcatcaccgc ctacatgacg gaaatcctga cccttcacgt agtagaagat aaactcacaa
     4261 atattctggc agcggtcgcc gatacgttcg attgaacgcg cacagaacag cgcggtaagt
     4321 acgctcggaa tagtgcgcga atcttccatc atgtaggtca tcagttgacg aacaatacct
     4381 tcgtattcct gatcgacttt tttatcttca cgataaatac gtaccgcttc gtcaatgtcc
     4441 atccgcgcga acgcgtccag cacgtcgtgc agcatctgga tggtatgacg gcccagcgac
     4501 tccagactta ccagcaacgg ctgatgctgc tgggagaatt tctccagcgc agtacggcag
     4561 attttgtccg ccacgtcgcc aatacgctcc agctcggcaa tggttttact gatcaccata
     4621 accagtcgca ggtcgctcgc cgtcggctga cgtttggcga taatgcgcac gcaggcttca
     4681 tcgatcgcca cttccatcat gttgacgttc ttgtcgcctt cgatcacgcg cttcgccaga
     4741 tcgctgtcct ggttatgcat cgcggtgatt gcatcagaaa gctgctgctc caccatgccg
     4801 cccatggtca tcacctgcgt gcggatactt tccagttcgg cgttgaactg gccggaaata
     4861 tgtttattaa gattgagact gtccataacg cactcctgaa tcaaccgtaa cgaccggtga
     4921 tgtagtcttc tgtttgtttc ttcgctggct tggtgaacag atcgtccgtg ttgctgaact
     4981 caatcaattc gcccaggtac ataaatgccg tgtggtcgga acaacgcgca gcctgctgca
     5041 tgttgtgggt gacgatcacc acggtgtaat cctgcttcag ttcggtgatc agctcttcaa
     5101 tacgcccggt agagatgggg tcgagcgccg aacacggttc gtcgagcagc agcacttccg
     5161 ggcgaatggc gataccacgc gcaatacaca gacgctgttg ctgaccacca gagagagagt
     5221 aaccgctctg gtgcaattta tctttggttt cgttccacaa tgcggctttg gtcaatgccc
     5281 actgcacgcg ctcgtccatg tcggcacggg agagcttctc aaacagacga acgccaaagg
     5341 cgatgttgtc gtagatggac atcgggaacg gcgtcggttt ctggaacacc atgcccactt
     5401 tcgcacgcag cagggcgata tcctgagagt tggtcaggat gttgtcgcca tcaagcagaa
     5461 tttcaccttc cgcacgctgc tccgggtaca gttcaaacat tttgttgaag gtacgcagca
     5521 gcgtcgattt accgcagccg gacggcccga taaacgccgt aacctggttt ttggcgatat
     5581 ccaggttgat gtttttcagg gcatggaatt tgccgtagta gaagttcaaa ttacgaacct
     5641 gaattttact cggggcagtt tcaaccatac tcattgcaat cttttcctca tcgtacctga
     5701 tgcttttcgc taatcaggct taccgtaggg ccgggtaagg cgttcacgcc gcatccggaa
     5761 aagtgttaac cgtgtttatt cttcgcaaaa acaacgcgcg ccagaatgtt cagcagcagg
     5821 acgcacaggg taatgatcaa taccccggcc caggccagtt gttgccattc cgcaaacggg
     5881 ctcatcgcaa acttaaagat cgtcaccggc aggttggcga tcggctgcat catgtccgtg
     5941 ctccagaact ggttggagag cgcggtaaac agcagcggcg cggtttcacc ggcaatacgg
     6001 gcaatcgcca gcaggatacc ggtcataatc ccggacaccg acgctttcag cgtaatcgcg
     6061 gagatcatct tccacttcgg tgtacccagc gcataagccg cttcacgcag gctgtccggc
     6121 accagtttca gcatgttctc agtggtgcgg ataacgatcg gcacctgcaa cagcgccagc
     6181 gcaattacgc ccgcccagcc ggagaagtgc tccatctgtg ccaccacgat ggtgtagaca
     6241 aacagaccaa ccacaatcga cggcgcggag agcagaatgt cgttaatgaa gcgaatcact
     6301 tctgccagcc aggatttacg accatattcc gccagataaa tccccgccat aatgcccagc
     6361 ggcgtaccga ataccgtggc ccacaaaatt aacagcccgc tacccgccag ggcgttcgcc
     6421 agaccaccac cttccgtatt gggcggcggc gtcatttcag tgaacagcgc cagcgacata
     6481 ccgtcgatac cgcgagtgat ggtggacatt aaaatccaga tcagccagaa caggccgaag
     6541 gccatcgtcg ccatcgagag cgtcagcgca atacggtttt tgaggcggcg acgcgcctgc
     6601 attttgcggc gagattcagc cagcgccgca gtggtttgca tttcaaccat agccattagc
     6661 gtgccccctc attcttagcc aggcgcataa tcataaactt cgatgcggcg aggacgatga
     6721 aggtaatcac aaacaggatc aggcccagtt ccatcagtgc ggcaacgtgc agaccggatt
     6781 ccgcttccgc aaattcgttc gccagcgcag aggtgatact attgcccggc atgtacagcg
     6841 aggcgctgtc gagctggtag gtgttaccga tgataaaggt caccgccatg gtttcaccga
     6901 gcgcgcggcc cagccccagc atgatgccgc cgataacacc atttttggtg aacggaagaa
     6961 cgatacgcca gataacttcc caggtggtgc agccaatacc gtaggccgac tctttcatca
     7021 tcaccggggt ttgttcgaac acatcacgca ttaccgccgc aatgtacgga ataatcatga
     7081 tggcgaggat cacgcctgcc gcgaggatac cgataccaaa tgcggggcca gagaacagcg
     7141 cgccaacaat cgggatattc gacatgatat tgccgaccgg ctcctgaaag taaacggcga
     7201 acagcggcgc aaagataaac aggccccaca tgccgtaaac gatacttgga atggctgcca
     7261 gcagctcaat ggcgataccc agcgggcgtt tcagccagcc aggcgcaagc tcagtcagga
     7321 acagggcgat accgaaactc accgggacgg cgatcagcag cgcgataaac gaagtcacca
     7381 acgtaccgta gatcggcacc agcgccccgt agatatcgtt cggtgcatcc cactctttgg
     7441 tccataggaa agccagacca aatttctgaa tgctcggcca ggaggagatg atcagagaga
     7501 caataatgcc acccaacatc aatagcacaa tcagcgccgc cagttttacc agcacgctga
     7561 aaattatgtc gcccttttta cccggtgggt taaaagcggg cttggttgca gccataagtt
     7621 actcttcagt taaacgcgtt tacaaagtca ttttgtaatg ccggatgcgg cgtaaaacac
     7681 cgtacccggc ctggagtttt attagtacag cggcttaccg ctactgtctt taatattggt
     7741 cttccacgca gcgcgaacct gttcaactac actatccggc aggctggcgt aatccaggtc
     7801 gttcgcctgt ttagccccgg ttttgtacgc ccagtcgaag aatttcagca cctctgtgcc
     7861 ttgttctggt ttcttctgat ctttgtggat cagaatgaac gtggtagagg taataggcca
     7921 tgcatcttcg cctttctggt tggtcagatc ctgagcgaag gttttgctcc agtctgcacc
     7981 ttttgctgca ttagcgaagt tttcttcggt cggactaacc ggtttaccat cagcggagat
     8041 cagtttggtg tacgccaggt tgttctgctt cgcgtaagca tattcaacat aaccaattgc
     8101 acccggcaga cgctgaacga acgcggcgat accgtcgtta cctttaccgc ccagaccgat
     8161 cggccatttt acggtagagc cagtaccaac gttgtttttc cactcttcgt tcactttcgc
     8221 caggtagctg gtgaagacga aggaagtccc ggagccatct gcgcggcgta ctacagcaat
     8281 gttttgtgaa ggcagtttca gacccggatt cagtttggcg atggcttcat catcccactt
     8341 cttgattttg cccaggtaga tgtcgccgag ggttttacca tccagcacca gttcgccaga
     8401 cttcagccct ggaatgttaa ccgccagcac cacgccgcca atcacggtcg ggaactggaa
     8461 cagaccttcc tgagccagtt tttcgtcaga cagcggcgcg tcagaggcac caaaatcaac
     8521 ggtattagcg ataatctgtt ttacgccacc ggaagaaccg ataccctggt agttaacttt
     8581 attaccggtt tctttctggt aagtgtcagc ccatttggca tacaccggcg cagggaaggt
     8641 tgcacctgca cctgtcaggc ttgcttctgc aaacacagag aaagcactca tcgataaggt
     8701 cgcggcgaca acagttgcga cggtggtacg cataactttc ataatgtctc ctgggaggat
     8761 tcataaagca ttgtttgttg gctacgagaa gcaaaatagg acaaacaggt gacagttata
     8821 tataaggaat atgacatttt tatgacagaa aggaattacc aaataactta ctgattttaa
     8881 atgtgaagat gaaaattcca catgccatct ttttgtcatg atgcatttaa aagtgataaa
     8941 gaaatgggtg agatgacaac gatgtgatga acaatcgttg tcagacctat tagcgcacaa
     9001 tctcaacagt tatcgtcgct gttgcagtaa attgccccgg ggctgggcga gctccagtaa
     9061 ggctcgcagg agcagctgaa aaagttacac tacctgattt ttcaccatac actgttgatt
     9121 tatccatgtc tgttggcaac tcaccaccgt tcactttgat ttccttatta tttttgtcgt
     9181 aaatttttat ccccacgtta gagtttgaag ttacgaccat tgaattgtcc gtattgggtt
     9241 cggtagcata aaaagacgta ctgatatcat cggtattcag tatccccagg ttttcacact
     9301 caatagtgac tgttttagta attttatgtg ttgttaccgc acttccaaca acagatgaaa
     9361 aatctgcagc tggtatttct ttaaaatcaa aacaaactgg ctttaaatta tttatttcgc
     9421 aactttgcgg agctgtaata tcgccccgaa tataaacatc agccatcggc tgtgagtaat
     9481 ccaccggggt gtcacggcta attgttccat acaatgtcac tatctttgtt tcaggaataa
     9541 tcaattttcc tgcaaatgtc ttcttgatat aaaaatatac tatcgcttta gccccactgg
     9601 atatacttaa aggttcaatg cggttgcaat tgtactttgt attaccagac gggttgttag
     9661 catgatattc aaaaggtgca ttcacatacc caaccccaag aatatctata gcaatcccaa
     9721 catcaaggtt cggattaaga tcataataat taatattaga gtgaacccca gcataagtta
     9781 atgcctggtt tatttttgac gtataataga tataggggaa ttctcctacg ttaccagtat
     9841 cacaatgaca gcgtccaaga tatgaggatc cattagttac agtggtgtta aatgtcgttc
     9901 caaccttatt ttcgttcgcg gtaataagat atcccga
//
LOCUS       DAJTRD010000122         9910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTRD010000122 DAJTRD010000000
VERSION     DAJTRD010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.122"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..326
                     /locus_tag="OO315_003526"
     CDS             <1..326
                     /locus_tag="OO315_003526"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892296.1"
                     /translation="EYDLFKNRETSLWKRKVPESAALAMTGTVYKLFGNRLLNARNQL
                     DKNLLEMAMQMVYGKDMVTKSGDILIDIQLNKDGSLQSKHYTLDVGEVINSYNIDPLC
                     ECFKY"
     gene            496..1629
                     /locus_tag="OO315_003527"
     CDS             496..1629
                     /locus_tag="OO315_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005052835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892297.1"
                     /translation="MKGGISMFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVG
                     PDILALPVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKS
                     KTIIFDLRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRS
                     VALAKTVAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISV
                     RNKYSPAFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAK
                     DRTISIVLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRV
                     LHVSNKFNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            2308..2526
                     /locus_tag="OO315_003528"
     CDS             2308..2526
                     /locus_tag="OO315_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5892298.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2744..2986
                     /locus_tag="OO315_003529"
     CDS             2744..2986
                     /locus_tag="OO315_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892299.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(3316..4245)
                     /gene="fdhE"
                     /locus_tag="OO315_003530"
     CDS             complement(3316..4245)
                     /gene="fdhE"
                     /locus_tag="OO315_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5892300.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(4242..4877)
                     /gene="fdoI"
                     /locus_tag="OO315_003531"
     CDS             complement(4242..4877)
                     /gene="fdoI"
                     /locus_tag="OO315_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5892301.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4874..5776)
                     /gene="fdoH"
                     /locus_tag="OO315_003532"
     CDS             complement(4874..5776)
                     /gene="fdoH"
                     /locus_tag="OO315_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5892302.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5789..8839)
                     /gene="fdnG"
                     /locus_tag="OO315_003533"
     CDS             complement(5789..8839)
                     /gene="fdnG"
                     /locus_tag="OO315_003533"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8252..8254),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5892303.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            9033..9866
                     /gene="fdhD"
                     /locus_tag="OO315_003534"
     CDS             9033..9866
                     /gene="fdhD"
                     /locus_tag="OO315_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5892304.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2484 a         2393 c         2540 g         2493 t
ORIGIN      
        1 atgaatatga cctgtttaaa aatagagaaa cctcactgtg gaaacgcaaa gtaccagaaa
       61 gcgcagcgct ggcaatgacg ggaacagtat ataaattatt tggtaatcgc ttactcaacg
      121 cccgcaatca gttagataaa aatctcctcg aaatggctat gcaaatggtt tatggcaaag
      181 atatggtgac aaagtccggg gatatattga ttgatataca attaaataag gatggcagtc
      241 tccagagtaa gcactacaca cttgatgtag gtgaagttat aaacagttat aatattgacc
      301 ctctttgtga atgcttcaag tattaatcaa acttacttac atgataaaaa tcctggtgac
      361 gttattagcc tgtatggcac tttagataac attccccttg tagttaagga atgcacgggt
      421 aaaaatcatc aaattaatgt taaagccaaa ttgccacaag aacgaagtga aaaagttgag
      481 gatatgtgtg aaagaatgaa aggtggcatc tctatgttta accacaccac aaaaacagct
      541 gataatatag agcataattt gcagctaagt tttctcgtgg gttcacgtcc gtcgatcacc
      601 aataaatact cagcaaaaga tgtaggtcca gatatacttg cattgcccgt caacgtatat
      661 catgcggatt ttaaaattaa aatcaataat gcagtggaga aggcttcatc aattgtgtta
      721 tgtgctgtgc aaaaaaatct ccatgcagca aggaaggttc ttagcgggat aataggaaat
      781 gtgccaaaaa ataataaatc aaaaacaatt atttttgatt tgcgttttct tagtgcaatt
      841 aagcagaaag tttttttggg cagtgaaaaa ccgttgtttg taaagcatac cgcaatgatg
      901 agggaggcat gtaaggaatt accgcagtca gtggaatatg ttcctttagc ctgtgaagcg
      961 catagccatc gcagcgtcgc ccttgccaaa accgtggctg cgcccatcat ggcgacaggt
     1021 gtgggcttgg cattaatgta tgcaacatca tcaggaccgt attatgcgca aagtctttct
     1081 ggttcgccgg atatcgagat gataaaaaat cgcatggctg aattgtttaa ccagtcggat
     1141 atttctgtgc gcaacaaata tagcccggct tttgataaat ggaatgaact ggtagataaa
     1201 ttaaatcacg aaagaaatac agttccgttt gcgtgtttga ccaccatttt agcgacggcg
     1261 ataaatgaaa ctcttgaggg ggatacgaat gttgccgttg tgatggggtg taaatctgcg
     1321 aaagatcgca cgatatccat tgtccttggc aactcgatgt tgcaaacact ccttgaaaaa
     1381 cgcctcgccg atagtggaga aatagaaaat ctttttgatc aacagggata ttttagttgc
     1441 gattcattaa cacccgaaga attaatgatg ttaaaagacc tttttgatat tcgagttctt
     1501 catgtctcta acaaattcaa tgttggattg caaggtaata tcaacactga tgttttacaa
     1561 gattcgtttt ttaaaaatgt cgatttcatt catgaatata actcgtatac cgtagggatg
     1621 ggagtctgac gagtaaaact cattaggatt atcagaattc ggacaatgca ttgcaaaaat
     1681 gacatacaaa gagagatgtg tgcaggtact tagccgcgag gggcatggcg aagccataca
     1741 gaaagtacca gtattcagaa ctccagtgaa agcggcatag tggccgcctg ctcaggagtt
     1801 ttgctatgac ccgtaatcgt gctgaacgtc gccaccctat gatgcgatta aaaaaactca
     1861 tcgtcgcgct gatgattgac gtcaatgcta tcggatgatg ccgggggtgt tccggaagga
     1921 attccttgtt tcccgaactg ttaccaacaa aattataacg atgagcaagt aaagaccggt
     1981 tggtggcagt actgaccggt ctgtgatgaa agattaattt caccttaaat cctggtgctg
     2041 ttaggatgct gacgtttttc ggctacacca ttcagattat gtaaataaat atccctgaat
     2101 ataatatatt catagcaccc ataactcatc ctgggcgata actaaaccat ttttgagtaa
     2161 taaatgaaac aactgcaacg ctcttattat ttctggaaaa cgcctcgcaa aaacaaaatg
     2221 acatgcaaaa atccgcattt ttttaaatgc ttttccggcg taattttcgc acatacatta
     2281 atgagtaata tatgtaaggt ggtggtaatg gcgatgaata cggtttttct tcatttatca
     2341 gaagaggcaa ttaaacggct gaacaagctt cggggctggc gtaaggtttc acgatctgca
     2401 attttacgcg aagcggttga gcaatatctg gagcggcagc aatttccggt gcgtaaagca
     2461 aagggaggca ggcaaaaggg ggaggtagtt ggtgttgatg atcagtgtaa ggagcataag
     2521 gaatgaaaaa aatttttcat tcttgaatat aaaaaacaga tgcccttatt ctggtattaa
     2581 tacaaggctg ttttacttga acttataata actgcaactg ttacatcgta tctggaaaac
     2641 gcctcgcaaa aataaaaaat gatgcgtaaa tgagctttct ttaaagattc ggcaagggta
     2701 cttttctctc catagattca aaaatgatat tggcgagata tttatgaact ctctggcagg
     2761 tatagatatg ggcagaattt tactcgattt atcgaatgag gtgattaagc aacttgatga
     2821 tctcgaggtg cagcgtaatc ttcctcgtgc ggacctatta agggaagcgg tagatcaata
     2881 cctgataaat caatcgcaaa cagcaagaac cagtgttcct ggcatctggc aagggtgtga
     2941 ggaagatggt gtcgaatatc agcgtaagct gcgcgaggaa tggtaatggg aaaaaatgat
     3001 gtcaatcaga ttgctgacaa cgtgcgcgtt gttcatgccg gatgcggcgt gaacgcctta
     3061 tccggcctac aaagtcatgc aaattcaata tattgaagtt tattggtagg cctgataagc
     3121 gcagcgcatc aggcaatcat acgcttgtca tcagtgacat acccataacc ttatcgatgt
     3181 gttggaggga agaacgaggc aaacagacaa ttgcggcatg atcttgaatg ggtaacgcgg
     3241 aatacgagag atggtgtcgt cacaccctat gagctgtaat ccgggcgacc tgcgccaccc
     3301 ggaaaacgtc agagattact ctccttcacc cggaaacagg aacgggttaa tggaactacg
     3361 ggcgtagcct tcttgctcca ttcgcgcgtc cagtaccaga gaggcgaggt catctgccac
     3421 ggcttcaact ttcggttctt tttcctgata aagaatcttc aggtaagtgc cgcagtcatc
     3481 gcagctttcg gctttaatcg cggcctgttc gtcatccagc gaccagtaat gcagtttgcc
     3541 gctctgctcg cagttgctgc attttacgcg cactacgtgc cattcggttt cacacaggtt
     3601 gcagtgcagg taacgcagac cctgagtggt gccaatttgc accatgctgg acaccggcat
     3661 agagccacat accgggcaat attgacgttg ttcgccgtat tcagcgcggg ctttgccggg
     3721 gatcagattg gccatctgcg cccagtagag cgacagcgca gcccagataa acggcgcttt
     3781 atcgctgctg acggacgaga aatcagaggc aaacagcgcg ctggccatat cttccagctc
     3841 ctgagtcgat gccttctcca gattctcaat cactgccagc gccgggccgc tcatttcagg
     3901 tttcagctca gcaattagcg ccatcagcag cttttgccag tgcttatcac gcggcagaac
     3961 gtgaatatcc agcgggggct tgccctgtgc gctggcttct ttaatgcgcg cggtcagatc
     4021 catctccagc ggatggtcgt acagcaccac tttctgggcg tgggcgataa gcgcagcaaa
     4081 gcgcaggtaa tcacccagcg gattattttc tgccagctcg cgcagacgct cggcgcggcg
     4141 gttgtataaa ttcttgagcc gagggaacaa taacggcgga atcatatccg ccgtacgttt
     4201 ctcgctcgaa cccagctcat cttgcgggat tatgcgaata ctcattcagc tttcttttcc
     4261 gttgtcttgc ggacctcacg gtaccagcgc gggtgatgtt tcttcgccca ggcgctggta
     4321 acccatcctt ccaccatggc ggtaatcgtg cctttcaccc aaagggcggc gtagatatgc
     4381 accatgataa ccacaattaa cgccactgcg gcaaatgaat gcagcattaa cgcgaatcgg
     4441 atcaccggga ttgagaaagc aggcgcaaaa taaggacgcc agataatcac gccgctcacc
     4501 agcagcagaa ccaggaaaat aatcgccgcc cagaaaacgc atttctgacc gaagttataa
     4561 cgcccggtgt cacctacttc ctcgttgacg acgatcttac gaatattctt cgcccaaaag
     4621 atatcatccc gattgattag gttttgatgc cagtaacgga aaaacatgat gatgaacgag
     4681 gcaaacataa ccacgccgac aaacgggtgc agaattcgcg ccagctgcgg tgtgcccatg
     4741 atttgcatca accagttgaa ggacgggaac aaaaagccca gcccgctcac cgccgccagg
     4801 atgaagcaga aggcggtgat ccagtggttg atacgttccg gcgcggtgta gcgcacgatg
     4861 gtgtcacgtc gtttcatttg cgctcctcgt ctttctcttc gtgcagatta ttctcttcct
     4921 catccgcacg gttcggaccg acacccacgt agtggaagat actggccgca aaggtagccg
     4981 caaagccaac agccgcgagc ggtttccaga tgcctttcca gaatttcacg gtttcgctga
     5041 tttccgggtt ctccggcaag ccatgataca gatttggctt gtcagcatgg tgcagcacgt
     5101 acatgacgtg tgtaccaccg acgcctgccg gatcgtacag acccgcattg tcgtaaccgc
     5161 gggttttcag ctcagcaacg cgctcgctcg ccagcgtttt catcgactct ttcgtgccga
     5221 agtgaatcgc gcccgttggg caggtcttca cgcaggctgg ttcttgccca accaccacgc
     5281 ggtcaacgca cagcgtacat ttgtagacgc ggttgtcttc cgggttgagg cgcggaatgt
     5341 cgaacggaca gcccgcaatg caataaccgc agccaatgca ctgctcggac tggaagtcga
     5401 cgataccgtt ggcatactga ataattgcac cttccgccgg gcacgctttc aggcagcctg
     5461 gatcggaaca gtgcatacag ccgtctttgc ggatcagcca ttccagtttg tcgttctgct
     5521 ccacttccga gaagcgcatt accgtccacg atttggcgct taaatcattg gggttgtcgt
     5581 acaccccaat gttattgccg acggtatcgc gaatgtcgtt ccactctgaa cacgccacct
     5641 gacaggcttt acagccgata caggtggtaa cgtcgatgag tttcgccact tcttcctgga
     5701 agtcccgcgc ctgaggcgcg ggggtcagac cgttagtcgc ggaacgacga atgatatctt
     5761 gagattgata agccatatgt cgtctccgtt acaccttttc cacattcaca aggaaggact
     5821 taaactccgg cgtctgcgtg ttcgcatcac caacgaatgg cgttaaagta ttggcaataa
     5881 agcctttttt agctacgcct tcatagcccc agtgaatcgg aataccgatg gtatcgatat
     5941 ctttgccgtt cgctttcagc gtgcgaatac gtttggtcac caccgctttg gctttgatat
     6001 agccacggtt ggaggagact ttcacggtat cgccctgggc aatgccaagt ttattcgcca
     6061 gcgactcccc gatttccaca aactgctctg gctgcaaaat cgcgttcaac agcgcgtgtt
     6121 tggtccagta gtggaagtgc tcggtcagac gataggtggt tccgacatac gggaacttat
     6181 cggctttacc caatgcttcg gcgtcgtctt taaagatacg tgcagctggg ttcgagataa
     6241 cgtttggatg cagcgggtta gttcccagcg gcgtttcaaa cggctcgtag tgttccggga
     6301 acggaccttc cgccatctta tcgagggcaa acagacgccc catgccttcc tgctgcatga
     6361 taaacggccc gacgccgcta cccggaggcg ctgcgctgta gtccggaata tcccagccgg
     6421 tccacttagt gccgtcccat ttcagcaact gacgcttcgg atcccacggg ttaccctgcg
     6481 gatctgcgga ggcgcggtta tacagaatgc ggcggttaag cggccatgcc catgcccagc
     6541 ccagcgtgtt accgaggcca gacggatcgg cgttatcacg acgcgccatc tggttgcctt
     6601 ccggcgtcca gctaccggcg aaaatccagc agccacagga ggttgtaccg tcatcgcgca
     6661 attgggcgaa cgagctaagt tgttggcctt tcttgacgat aaccgcaccg gttgccggat
     6721 cggtaatatc ggccagcgcc ttaccgttgc tctccatcgc tacttcttcc gacttcggct
     6781 catgcggaat ggcgtagttc caggtcatgt tcagcacctg atccgggttc gcgccaccct
     6841 gttcggcata catcttgcgc aagcgcagga agataccgga gaggatctca ccgtcagtca
     6901 gcgcaatccc cggggcgtcc gcacctttcc agtgccactg caaccagcga cctgagttaa
     6961 cgattgaacc gttctcttct gcgaagcagg tcgatggcag acggaacact tcggtctgga
     7021 tcttcgacga gtcaacttcg ttcagctcac cgtggttctg ccagaagtta gaggtttcag
     7081 tgttcagtgg gtcgatggtg acgaggaact tcagtttcga cagacaaccg atcactttgt
     7141 ttttgttcgg gaatgaggca acagggttaa agccctggca gatatagcca ttgaccttgc
     7201 cctctttcat catctcgaag tattgcagga cgtcgtagcc tttatcccac ttcggcaacc
     7261 agtcaaagcc ccagctattt tccgccgtcg ctttatcacc aaagaaggcc ttcatcatag
     7321 agacgaagaa tttcgggtag ttgccccagt agtttacctg gccttccagc agcggttttg
     7381 gcgtgttggc ggtaaggtag gtttgcagat cggtctgctt ctcgcttggc agcgtcatgt
     7441 aacctggcag actctgcgac agtagcccca ggtccgtcag cccctgaata ttggagtgac
     7501 cgcgcagggc gttaacgccg ccgccggcca tccccatatt gccgagcagc agctggatca
     7561 tcgccatcgt acgaatgttt tgcgcaccaa cggagtgttg cgtccagccg agggcataca
     7621 ggaacgaggc agttttatcg tgagcactgg tttctgcgat gtattcgcag actttcagga
     7681 acgcgtcttt tggcgtacca cagatgtttt caaccacatc tggcgtgtag cgggaaacgt
     7741 gctgtttcag caagttccac acgcagcgcg gatgttgcag cgtggtatcg cgtttggcga
     7801 agccgttttc gtccagttca taagtccagg aggatttatc gtacttgcgt ttttccgcgt
     7861 cgtagccggt gaacaggcca tcttcaaagc cgtaatcctc acgcacgatc aggctggcgt
     7921 tggtataggc ttcggtgtat tcgtggttga atttttcatt gttcagcagg tacagcaata
     7981 cgcctgacag gaaagcaatg tcagtaccgg aacgaatagg ggcatagtag tcagctaccg
     8041 ccgccgtacg cgtaaagcga ggatcgatca caatcagctt cgcgccgttg tgaattttgg
     8101 cttccatcgc ccagcggaac ccgaccgggt gagcttcagc ggcgttaccg cccatcacca
     8161 cgacgaggtt ggcgttcttg atgtcgaccc agtggttggt catcgcaccg cgaccaaatg
     8221 ttggagcaag acttgctacc gttggtccgt gtcagacacg cgcctggttg tcgaccgcga
     8281 gcatacccag cgcgcgggag aatttttgcg ttaaatagcc ggtttcgtta ctcgacgcgg
     8341 aagcacacag catcccggtg gagagccagc ggttaacagt cacgccttcg gcgttttgcg
     8401 caatgtagtt agcatcgcgg tcttctttca tcagtttggc gatgcgatca aacgcctctt
     8461 cccaactgat ttgctgccat ttatcagaac ctggcgcacg gtattccgga aacttcagac
     8521 ggctttcgga gtggatgaaa tccaccaggc cagcgccttt cggacaaagt gcaccgcggt
     8581 tgaccgggtg atccggatcg ccttcgatat ggaagataga tgctttggcg ttttttgctc
     8641 cgtcaccgag gctgtacatc aacagcccac agcctacgga acaataggtg caggtattac
     8701 gggtttcgcg ggtgcgcagc agtttatact gccgggtttc cgcgagtgct acgctgggtg
     8761 caaaacccag tgccgctgcc gtggtgcctg ccataccgcc agcgcagatc ttaaagaact
     8821 gccttctgct gacctgcatg gattgctcct tgtttcgaca ttgtcacgtc ccatttacat
     8881 tcgcttgctg cgtgtgcagg gagtgggagt tatttttctt tgcggaaggg gccgcaaagg
     8941 tccagaattg gctcaatttc cctccatcta ggaagggttt gtaacagaat accataatgt
     9001 tggtgtgtat gttcttatct ggttaagaga aagtgaaaaa aacacagcga aaagaaatcg
     9061 aaaatgtgac aaatatcaca ggtgttcgtc aaattgagtt atggcggcgt gatgatttac
     9121 aacacccacg gttagatgag gtcgcggaag aagttcccgt tgcgctggtc tacaacggca
     9181 tttcgcatgt ggtgatgatg gcgtcgccca aagaccttga gtactttgcg ctcggttttt
     9241 cgctttccga ggggataata gaaagtccgc gcgatatctt cggcatggat gtcgttcctt
     9301 cctgtaatgg tcttgaagta caaattgagc tttccagccg ccgctttatg gggttgaagg
     9361 agcgccgccg ggcgctggcg ggacgtacgg gctgtggcgt atgcggcgtg gagcaactta
     9421 atgacatcgg aaaaccggtg cagccgctac cgttcaccca gacgtttgat ctcaacaaac
     9481 tggatgatgc attacgtcat ctcaacgatt ttcagccagt ggggcgactg actggttgta
     9541 ctcacgccgc tgcctggatg ttgccatctg gcgaactggt cggcgggcat gaagacgtgg
     9601 gtcgccatgt ggcgctggac aaactgttag gccgccggtc acaagaaggg gaaagctggc
     9661 agcaaggtgc ggtactggtt tccagtcgtg ccagttatga aatggtgcaa aagtcggcga
     9721 tgtgcggcgt agagattttg tttgcggtgt ctgccgcgac cacgcttgct gtagaagtgg
     9781 ctgagcgctg taatctgaca ctggtaggtt tttgtaaacc gggtcgggca acggtttata
     9841 cccatccgca gcgtttgagc aattaattaa aataagtcct gcgatatata ttaaattgca
     9901 ggacttattc
//
LOCUS       DAJTRD010000123         9807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTRD010000123 DAJTRD010000000
VERSION     DAJTRD010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.123"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            429..1547
                     /locus_tag="OO315_003535"
     CDS             429..1547
                     /locus_tag="OO315_003535"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5892305.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FMALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1613..1861
                     /locus_tag="OO315_003536"
     CDS             1613..1861
                     /locus_tag="OO315_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5892306.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1926..2294
                     /locus_tag="OO315_003537"
     CDS             1926..2294
                     /locus_tag="OO315_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5892307.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2388..3041
                     /gene="nfsB"
                     /locus_tag="OO315_003538"
     CDS             2388..3041
                     /gene="nfsB"
                     /locus_tag="OO315_003538"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5892308.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            3149..4396
                     /gene="ybdG"
                     /locus_tag="OO315_003539"
     CDS             3149..4396
                     /gene="ybdG"
                     /locus_tag="OO315_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5892309.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="OO315_003540"
     CDS             complement(4464..5840)
                     /gene="pheP"
                     /locus_tag="OO315_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5892310.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="OO315_003541"
     CDS             complement(5941..9084)
                     /gene="cusA"
                     /locus_tag="OO315_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5892311.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(9096..9614)
                     /gene="cusB"
                     /locus_tag="OO315_003542"
                     /pseudo
     CDS             complement(9096..9614)
                     /gene="cusB"
                     /locus_tag="OO315_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2400 a         2716 c         2381 g         2310 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagggttg atagaaatat
       61 ctcctggggc ttcatctttc cagacctcag agtaaacctg aaatcggaaa gcttcaggca
      121 cccgcagtta tcttaccctt cctcaacaaa atagctgttt atttatacag caaatcctgt
      181 ttcggacttc tcactcccca tttgatcaac tgaagctgaa cccgtttttt tctgcatgcc
      241 tcaatcgtca aaattcttcg ccaccagcca gctctgccat aacttgccac attgaaaaag
      301 tagccaaagt gcttcaggca ccatcagttc accatcacca aaaaaaagaa acattccaga
      361 acttccgcat ctcgcccagc cgctccgact tctatactga atagaaaacg tcaacttaag
      421 agaaacctat gccattaccc gattttcatg tttctgaacc ttttaccctc ggtattgaac
      481 tggaaatgca ggtggttaat ccgccgggct atgacttaag ccaggactct tcaatgctga
      541 ttgacgcggt taaaaataag atcacggccg gagaggtaaa gcacgatatc accgaaagta
      601 tgctggagct ggcgacggat gtttgccgtg atatcaacca ggctgccggg cagttttcag
      661 cgatgcagaa agtcgtattg caggcagccg cagaccatca tctggaaatt tgcggcggtg
      721 gcacgccccc gtttcagaaa tggcagcgtc aggaggtatg cgacaacgaa cgctatcaac
      781 gaacgctgga aaactttggc tatctcatcc agcaggcgac cgtttttggt cagcatgtcc
      841 atgttggctg tgccagtggc gatgacgcca tttatttgct gcacggcttg tcacggtttg
      901 tgccgcactt tatggccctt tccgccgcgt cgccatatat gcagggaacg gatacgcgtt
      961 ttgccccctc acgaccgaat attttttccg cctttcctga taatggcccg atgccgtggg
     1021 tcagtaactg gcaacaattt gaagccctgt ttcgctgtct gagttacacc acgatgatcg
     1081 acagcattaa agatctgcac tgggatattc gccccagtcc tcattttggc acggtggagg
     1141 ttcgggtgat ggataccccg ttaaccctta gccacgcggt aaatatggcg ggattaattc
     1201 aggccaccgc ccactggtta ctgacggaac gcccgttcaa acatcaggag aaagattacc
     1261 tgctgtataa attcaaccgt ttccaggcct gtcgctatgg tctggaaggc gtcattaccg
     1321 atccgcacac tggcgatcgt cgaccgctaa cggaagatac cttgcgattg ctggaaaaaa
     1381 tcgccccttc agcacataaa attggcgcat cgagcgcaat tgaagcactg catcgccagg
     1441 tcgtcagcgg tctgaatgaa gcgcagctga tgcgcgattt cgtcgccgat ggcggctcgc
     1501 tgattgggct ggtgaaaaag cattgtgaga tctgggccgg tgactaagcc gggaattgcc
     1561 ttacagaaga atatccctga caatggtgtt ttaacttaca tttaacaatg ttatgaaaca
     1621 ccctttagaa accttgacca ccgcagcagg cattttgctg atggctttcc tctcttgcct
     1681 gctgctgccc gcccccgcac tggggctgac gctggcacaa aaactggtga ccatgttcca
     1741 tctgatggat cttagccagc tttacacttt attgttttgt ctgtggtttt tagtgctggg
     1801 cgctattgag tattttgttc tgcgctttat ctggcgacgc tggttctcgc tggcggatta
     1861 aacgtcgatt atcgccgaac ttcgcggcat actttgctta ttctccttcg cctaaaggaa
     1921 cgtttatgga taagcaatca ctgcacgaaa cggcgaaacg cctagctctt gagttaccct
     1981 ttgtcgagct ttgctggcct tttggcccgg agttcgatgt ttttaaaatt ggcggcaaga
     2041 tttttatgct gtcgtcggag ctacgcggcg tcccctttat caatctgaag tccgatccac
     2101 aaaaatccct gttaaatcag caaatatacc caagcattaa gccagggtat cacatgaata
     2161 aaaagcactg gatttcggtg tattccggcg aggaaatctc cgaagcgtta cttcgcgatc
     2221 tgatcaacga ttcgtggaat ctggtggttg atggtctggc taaacgcgat caaaaaagag
     2281 tgcgtccagg ctaaagcggg aatctatagc gcatttttct cgcttaccat ttctcgttga
     2341 accttgtaat ctgctggcac gcaaaattac ttacacatgg agtctttatg gatatcattt
     2401 ctgtcgcctt aaagcgtcat tccactaagg catttgatgc cagcaaaaaa cttaccccgg
     2461 aacaggccga gcagatcaaa acgctactgc aatacagccc atccagcacc aactcccagc
     2521 cgtggcattt tattgttgcc agcacggaag aaggtaaagc gcgtgttgcc aaatccgctg
     2581 ccggtaatta tgtgttcaac aaacgtaaaa tacttgatgc ctcgcacgtc gtggtgttct
     2641 gtgcaaaaac cgcgatggac gatgcctggc tgaagctggt tgttgaccag gaagatgccg
     2701 atggccgctt tgccacgccg gaagcgaaag ccgcgaacga taaaggtcgc aagttcttcg
     2761 cctatatgca ccgtaaagat ctgcatgatg atgcagagtg gatggcaaaa caggtttatc
     2821 tcaacgtcgg taacttcctg ctcggcgtgg cggcgctggg tctggacgcg gtgcccatcg
     2881 aaggttttga cgccgcaatc ctcgatgcag aatttggtct gaaagagaaa ggctacacca
     2941 gtctggtggt tgttccggta ggtcatcaca gcgttgaaga ttttaacgct acgctgccga
     3001 aatctcgtct gccgcaaaac accaccttaa ccgaagtgta attctctctt gccgggcatc
     3061 tgcccggcta tttcccctca gattctcctg atttgcataa ccctgtttca gccgtcatca
     3121 taggctgctg ttgtataaag gagacgttat gcaggattta atatcccagg ttgaagattt
     3181 agcgggtatt gagatcgatc acaccacctc gatggtgatg attttcggta ttatttttct
     3241 gaccgccgtc gtggtgcata ttattttgca ttgggtggta ctgcggacct tcgaaaaacg
     3301 tgccatcgcc agttcacggc tttggttgca aatcattacc cagaataaac tcttccaccg
     3361 tttagctttt accctgcagg ggattatcgt caatattcag gcggtattct ggctgcaaaa
     3421 aggcaccgaa gcggcagata ttctgactac ctgcgcgcag ttgtggatca tgatgtatgc
     3481 gctgctttca gtcttctcgt tgctggatgt tattttgaat ctggcgcaga aattcccggc
     3541 ggcatctcag ttaccgctga aagggatatt tcaggggatt aaactgatcg gcgcaatcct
     3601 ggtcggcatt ttgatgatct cgctgctgat tggtcagtcg ccagcgattc taatcagcgg
     3661 tcttggtgca atggctgccg tgctgatgtt ggtatttaaa gatccgattc ttggtctggt
     3721 ggcaggtatt cagctttccg cgaacgatat gctgaaactg ggcgactggc tggaaatgcc
     3781 gaaatacggc gcggatggcg cggtgatcga tattgggtta accaccgtca aagtgcgtaa
     3841 ctgggacaat accattacca ctattcccac ctggtctctg gtttctgact cctttaaaaa
     3901 ctggagcggg atgtcagcat ctggcgggcg acgtattaag cgcagtatca gtattgatgt
     3961 caccagtatt cgttttcttg atgaagacga aatgcaacgt ctgaataaag cgcatttgtt
     4021 aaagccttat ttaaccagcc gccatcagga aattaatgag tggaatcgcc agcaaggttc
     4081 tacggagtcg gtattaaacc tgcgccgaat gaccaatatt ggaacctttc gtgcctatct
     4141 gaacgaatat ctgcgtaacc atccgcggat tcgtaaagat atgaccttaa tggtacgcca
     4201 actggctcca ggtgataacg gtttaccgct cgagatctac gcgtttacta acaccgtggt
     4261 gtggctggaa tatgaaagca ttcaggctga tatattcgat cacatatttg cgattgtcga
     4321 agagtttggt ctgcgacttc atcagtcgcc aaccggcaat gatattcgct ctctggcggg
     4381 tgcatttaag cagtaattaa aaaaaccgct ctcatcgaat ggatgagagc ggacatcgtt
     4441 ttgtcggatg tagcgtgaat gccttatttc cgacgcagcg ttttaaatgc cacaaacagg
     4501 aatacaatcc acaccggcag caggatcgct gacaagcgca tatcatccat cgtgcacatc
     4561 agcagcaaaa tcatgccgag gaaggcgatg caaagatagt tgccgaacgg atagagcagc
     4621 gccttaaact gtgtttcacg cccctgacgt cgcatcgccg cgcgaaaacg cagatgcgcc
     4681 aggcagatca taatccagtt caacagcaac gttgctacca ccagcgccat cagcagacca
     4741 aacgcttttt gcggcagcag atagttgatt aacaccacca gcgaagtgat cgctccggaa
     4801 agcatcagcg agttaatcgg cacaccgcga cggctgacgc gagtcaaaaa cttcggcgca
     4861 ttaccctgca cagaaaggcc aaacagcatg cggctgttag agtaaacccc gctgttatac
     4921 accgacagcg atgccactag aatgacgaag ttcagcgcag aagctaccac attgctgtcg
     4981 agattatgga aaatcatcac aaacgggcta ctgttggatt tcacttccac ccacggatag
     5041 agtgccagta aaaccaccag tgaaccgata taaaacagca ggatgcgata caccacctga
     5101 tttaccgctt ttggaatgct tttttccgga tcgcgcgctt cagcggctgt aatcccaatc
     5161 agctccagac cgccgaagga gaacataatt accgccagcg acaaaatcag cccattccag
     5221 ccggtggcga agaaaccacc gtagcgccag aggttgtcga tactggcttt ctcgccgccg
     5281 taaccagaaa acagcagcca caggccaaag ccgatcatac cgatgatcgc cagcacttta
     5341 atcagcgcaa accagaactc ggtttcgcca tataagcgca cgttcaccag gttaacggcg
     5401 ttgatgataa taaagaaggc ggcagcccaa atccacgttg gaacatccgg gaaccagtac
     5461 tgcatataga tgcccgcagc ggtcagctct gccattccca ccagcacgaa cattacccag
     5521 tagttccagc cagagaggaa gcccgcaaac ggtccccagt atttataggc aaagtgggca
     5581 aatgaaccgg ataccggctc ttcgaccacc atctcgccaa gctggcgcat aatcaggaaa
     5641 gcgatgatcc cggcgacgcc gtagcccagc aatacagccg gacccgccat ctgaatcgcc
     5701 gggccaatgc caagaaacag accagtacca attgcgccac ccagcgcaat cagttgaata
     5761 tgacggttat gtaatccgcg atgaagcgtc ggctcttgat tcgacgcagt atcttccgat
     5821 acggttgacg cgtttttcac gcctttcccc tgtgtgtctt ttttgttgag gggcaccttt
     5881 taacatttag tgcccatcgt cgcaagacac aatccacacg gttaaacgag gtatcctgct
     5941 ttaattccgt acccgatgtc ggtgcagcca catcagctta tacgccgccg ggataataaa
     6001 cagcgacagc aaaggtgcgg tgatcatgcc gccaatcatc ggtgcggcaa tccggctcat
     6061 cacctctgaa ccagcccccg ttccccacag aatcggcagc agaccggcga taatcaccgc
     6121 caccgtcatc gctttcgggc gcacgcgcag gaccgcgccg tgatacaacg cctcatccag
     6181 cttctgctcg ctgaatgttt gtggattatt caacgacggc tcggcctcta tggcgtgacg
     6241 taaatacatc agcatcacca cgccaaattc ggcggcgacc ccggcaaggg caataaagcc
     6301 ggtacccgtc gccacagaaa gatgaaagcc catccaccac agtaaccaga tgccgcccac
     6361 cagcgcaaac ggtacgctgc tgataatcag caacgcttcg cccacccgac ggaatgccag
     6421 atacaacagc acgaagatga tcatcagtgt catcggcacc atgagtttca gcttatggtt
     6481 ggcgcgctcc agtagctcga actgcccgga gaatgccacg ctggtgcccg gttttaactg
     6541 cactttttca gctatcgctt tttgcaaatc gtgaaccacc gacaccatgt cacgatcgcg
     6601 ggcatcgata taaatccagc tcgtcgggcg cgcattctcg gttttcagca tcgacggtcc
     6661 ggtagagact ttaacgtcgg ccacgtctgc cagggtgatt tgctgcttca tcggcgtcag
     6721 gatcggcaac tggcgcagtg cctgcggact atcgcgccag ctttgcggat aacgcagatt
     6781 aattggataa cgggcaatcc cttccaccgt ttcgccaacc atcgccccgc ccaccgcaga
     6841 agtcacaaac aactgcacat ccgccaccgt cataccgtaa cgtgcggctt tttcacggtt
     6901 aatctcaacg ttgatatagc gcccaccttc cagccgctca gcaagcgcag aagctacgcc
     6961 tggcaccgtt cgcgccactt cttcaatttg ctcagccatc gcgtcgatat ccgccagcac
     7021 agtgccggaa actttaatgc cgatggggct tttaatgccg gttgagagca tatcgatacg
     7081 gttacgaatt ggcggcaccc acagattcgc cagccccggc agacgcacgg tgttatccag
     7141 ttcctcaatg attttgtcca ttgtcatgcc tggccgccac tgatcctgtg gcttaagctg
     7201 gatggtcgtt tctaccatct ccagcggagc agaatcggtg gcggtttccg ctttcccggt
     7261 tttgccaaat acccgcgcca cttcaggtac gctcataatt agcttgtcgg ttttttgcag
     7321 catactcgcc gcctctgctg cggaaatccc cggcagcgtc gatggcatat acaacaagtc
     7381 gccttcattg atctgcggta aaaattcccc gccaacttta ttgagcggcc agagaaccgt
     7441 cagcaccgaa agcgccgcca ccagcagcgt ggttttcggc cagtgcagta ctttcagcaa
     7501 cagcggatga taaacacgaa tcaaaaagcg attgagcgga ttactgcttt ccggcggaat
     7561 tttgccacgg atccagtaac ccatcaggat agggatcact acgatcgcca gcagcgccgc
     7621 acccgccatc gcatacgttt tggtgaacgc caacgggcca aacagacgcc cttcctgccc
     7681 ttccagggtg aagatcggga taaacgacaa cgtgataatc agcagactga taaacagcgc
     7741 cggccccact tcaacagacg catcggtgat cacctgccag cgcgttttat tatccagcgt
     7801 ggcgtcagga tgctggtgct gccactcttc cagccgttta tgcgcattct cgatcatgac
     7861 gatagcggca tcgaccatcg ccccgacggc aatcgcaatg ccacccagcg acataatatt
     7921 ggcattcagc ccctggaagg gcatgacaat aaaagcaata cacaacccca gcggcaacga
     7981 aataatcgcc accagcgccg agcgcacatg ccagagaaac ggcgcgcaga ctaccgccac
     8041 cacaataaac tcttccagca acttgccgct gaggttgtcg atggcccggt caatgagctg
     8101 gctgcggtcg tatgttgtaa ctatctccac gccttccggc agactacttt tcagcgtttc
     8161 cagtttgtcc ttcacggcgg cgatcacttc tcgggcgttt ttgccggatc gcaggatcac
     8221 caccccgccc gccacttcgc cttcgccgtt tagttcggca atgccccggc gcatcttcgg
     8281 gccaatctgg accttcgcaa catcgcgcag ataaacgggc acgccatttt cactggcttt
     8341 taaaacgatg tgattaaagt cgtcgagcgt ttgtagatag ccgctggcgc gcaccatata
     8401 ttctgcttcc gccagttcga ttgacgaacc gcccgcttcc tggtttgaag catccagcgc
     8461 gctttttact tcggcgagac tgatgccata ctgcgccagg cgctgaggat cgataaccac
     8521 ctgatactct ttcaccacac cgcctaccga cgccacttcc gcaacgtcag gaatggtttt
     8581 cagctcatat ttgagaaacc agtcctgcaa tgagcgtaaa tcggcaagat cgtgcttacc
     8641 gctgcgatcc accagtgcat attcatagat ccagccaaca cccgtggcat ctggtcccag
     8701 ctcggcgctg actcccgcag gcagcttacc ctgtacctgg ttgaggtact ccagcacccg
     8761 cgaacgcgcc cagtacggat cggtgccatc ttcgaaaatg acatacacat aagagtcgcc
     8821 aaactgcgag aaaccacgca cagtcttcgc gccaggcacc gacaacatgg tggtggttag
     8881 cggataagtc acctgatttt caacgatttg cggtgcctgg ccgggatagc tggttttaat
     8941 aatcacctgc acatcggaga gatccggcaa cgcatccact ggcgtattaa tgatggtcca
     9001 ggtgccccag atgctcagaa acaacgcgcc catcagcacc aaaaaacggt tcgccaccga
     9061 gcgacgaata atccattcaa tcattggtta ttccctcaat gcgcatgggt agcactttca
     9121 gagcgcatcc gctccagtgc gccagaaata ttggcttcgg aatcaatcag gaacaggccg
     9181 ccggaaacca ccttttcacc ttccgccaga ccagagcgta atgcggtgac gccttgcgat
     9241 gcctggaaaa cagcaacgcg tttcggtaca aagcgcccgt cggcatcaac ggtaatcacc
     9301 cgctgttcgt tgccggtatc aatcagcgcc tgtgacggaa tgagcagcat cggttcgctg
     9361 gcggtgttga gttgcaacca ggcgttcatg cccggtttta gcgcctcgtc ggcgttgtcg
     9421 acttccagac gcagctgcag cgtgcgggtc gcggcatcca cgccaggtag cagcgtccat
     9481 ttgcggatgg tgagtgtttt atccggtcgc gccggaacgg tgagggtaaa ctgcgaggca
     9541 tctttcacca gccaggcgat ggactccggg atcgcagcag tgacccacac cgggtccata
     9601 ccctgaattc gcgattacgc gcaatccccc tgagaaccgc catgaacaag ggtttcatga
     9661 tttgtgattt tagtttggta cgcaatttgg tacacaacac tattttcact tcagcgggta
     9721 gtcatcaaac tcaccataac gagcatcgtt gatgatgtat gtgatcacca caaatatcac
     9781 atctggacta gttccgtctt catgcgc
//
LOCUS       DAJTRD010000124         9764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTRD010000124 DAJTRD010000000
VERSION     DAJTRD010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.124"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            390..2021
                     /gene="oppA"
                     /locus_tag="OO315_003543"
     CDS             390..2021
                     /gene="oppA"
                     /locus_tag="OO315_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5892312.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2107..3027
                     /gene="oppB"
                     /locus_tag="OO315_003544"
     CDS             2107..3027
                     /gene="oppB"
                     /locus_tag="OO315_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5892313.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3042..3950
                     /gene="oppC"
                     /locus_tag="OO315_003545"
     CDS             3042..3950
                     /gene="oppC"
                     /locus_tag="OO315_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5892314.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3962..4975
                     /gene="oppD"
                     /locus_tag="OO315_003546"
     CDS             3962..4975
                     /gene="oppD"
                     /locus_tag="OO315_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5892315.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4972..5976
                     /gene="oppF"
                     /locus_tag="OO315_003547"
     CDS             4972..5976
                     /gene="oppF"
                     /locus_tag="OO315_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5892316.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6029..6358)
                     /locus_tag="OO315_003548"
     CDS             complement(6029..6358)
                     /locus_tag="OO315_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5892317.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6393..7853)
                     /gene="cls"
                     /locus_tag="OO315_003549"
     CDS             complement(6393..7853)
                     /gene="cls"
                     /locus_tag="OO315_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5892318.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7996..8169
                     /locus_tag="OO315_003550"
     CDS             7996..8169
                     /locus_tag="OO315_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5892319.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8224..9477)
                     /gene="kch"
                     /locus_tag="OO315_003551"
     CDS             complement(8224..9477)
                     /gene="kch"
                     /locus_tag="OO315_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5892320.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2562 a         2284 c         2430 g         2488 t
ORIGIN      
        1 aaagatgttt gttaaggcat ggatgcaagc tatagattct gatacggtca ataaaagaga
       61 atcgcttaac aattttgcaa aatgtattgg cgagtaagaa ccgcatttgg tactttccgg
      121 gcaaccgcca gacgattctt tattggtaat gagaataatt aacaattaaa gagcgtcgcg
      181 aaagaataat gtgtctcgac aggggagaca cagtacgaat cgacataagg tgatcgtctg
      241 aatcaccaga ataaataaag tcggtgatag taatacgtaa cgataaagta acctgacagc
      301 agaaagtctc cgagcttgtg cagggtccca atccgggatt acacatgctg gttaatacca
      361 gtaattataa tgagggagtc caaaaaacaa tgaccaacat caccaagaga agtttagtag
      421 cagctggcgt tctggctgcg ctaatggcag ggaatgtcgc gctggcagct gatgtacccg
      481 caggcgtcac actggcggaa aaacaaacac tggtacgtaa caatggttca gaagttcagt
      541 cattagatcc gcacaaaatt gaaggtgttc cggagtctaa tatcagccga gacctgtttg
      601 aaggcttact ggtcagcgat cttgacggtc atccagcacc tggcgtcgct gaatcctggg
      661 ataataaaga cgcgaaagtc tggaccttcc atttgcgtaa agatgcgaaa tggtctgatg
      721 gcacgccagt cacagcacaa gactttgtgt atagctggca acgttctgtt gatccgaaca
      781 ctgcttctcc gtatgccagt tatctgcaat atgggcatat cgccggtatt gatgaaattc
      841 ttgaagggaa aaaaccgatt accgatctcg gcgtgaaagc tattgatgat cacacattag
      901 aagtcacctt aagtgaaccc gttccgtact tctataaatt acttgttcac ccatcaactt
      961 caccggtgcc aaaagccgct atcgagaaat tcggcgaaaa atggacccag cctggtaata
     1021 tcgtcaccaa cggtgcctat accttaaaag attgggtcgt aaacgaacga atcgttcttg
     1081 aacgcagccc gacctactgg aacaacgcga aaaccgttat taaccaggta acctatttgc
     1141 ctattgcttc tgaagttacc gatgtcaacc gctaccgtag tggtgaaatc gacatgacgt
     1201 ataacaacat gccgatcgaa ttgttccaga agctgaaaaa agagatcccg gacgaagttc
     1261 acgttgatcc atacctgtgc acttactatt acgaaattaa caaccagaaa ccgccattca
     1321 acgatgtgcg tgtgcgtacc gcactgaaac taggtatgga ccgcgatatc attgttaata
     1381 aagtgaaagc gcagggcgac atgcccgcct atggttatac tccaccgtat actgatggcg
     1441 caaaattgac tcagccagaa tggtttggct ggagccagga aaaacgtaac gaagaagcga
     1501 aaaaactgct ggctgaagcg ggttataccg cagacaaacc gttgaccatc aacctgcggt
     1561 ataacacctc cgatctgcat aaaaagctgg cgattgctgc ctcttcattg tggaagaaaa
     1621 acattggtgt aaacgtcaaa ctggttaacc aggagtggaa aacgttcctc gacacccgtc
     1681 accagggcac ttttgatgtg gcccgtgcag gctggtgtgc agactacaac gaaccaactt
     1741 ccttcctgaa caccatgctg tcgaacagct cgatgaatac cgcgcattat aagagcccgg
     1801 cctttgacag cattatggcg gaaacgctga aagtgactga cgaggcgcag cgcacagctc
     1861 tgtacactaa agcagaacaa cagctggata aggattcggc cattgttcct gtttattact
     1921 acgtgaatgc gcgtctggtg aaaccgtggg ttggtggcta taccggcaaa gatccgctgg
     1981 ataataccta tacccggaat atgtacattg tgaagcacta atggcaatac gtggggcagg
     2041 agtgtcctgc tccacggtgt ctgattttta tcgcattaca gaaggcacag gccagaaggt
     2101 agggcaatgt taaaatttat tctacgtcgt tgtctggaag cgattccgac gctatttatt
     2161 cttattacta tttcgttctt tatgatgcgc ctcgcaccgg gaagcccttt taccggcgaa
     2221 cgtactttac cgccagaagt gatggccaat atcgaagcga aatatcatct taatgatccg
     2281 atcatgacac agtatttcag ctacctgaaa caactggcgc atggcgattt cggtccatcg
     2341 tttaaatata aagattattc ggtcaatgac ctggtggcat ccagttttcc cgtttctgcc
     2401 aaactgggag ccgccgcatt tttccttgcg gtaatactgg gtgttagtgc tggcgttatt
     2461 gccgcattaa aacaaaacac caaatgggac tataccgtga tggggctggc aatgaccggg
     2521 gttgttatcc ccagttttgt ggttgcgcca ttattagtca tgatatttgc gatcattttg
     2581 cattggctgc cgggcggtgg ctggaatggt ggggcgctta aattcatgat attgccgatg
     2641 gtggcgttgt cactcgccta tatcgccagt attgcgcgta ttacccgtgg ctctatgatt
     2701 gaagtattac actccaactt tattcgtact gcccgggcga aagggttacc tatgcggcgg
     2761 atcattttac gccatgcatt aaaacctgct ctgttacccg tgctctccta tatgggcccc
     2821 gcatttgtcg gcattattac cggttctatg gtcatcgaaa ccatttatgg tttgccgggg
     2881 attgggcaac tgttcgttaa tggcgcattg aaccgtgact attccttagt gttaagcctg
     2941 accatcctgg ttggtgcgtt aaccattttg tttaatgcca ttgtcgatgt gctatatgcg
     3001 gttatcgacc cgaaaatccg ttactgatac tggagctcgc gatgatgtta agtaagaaaa
     3061 acagcgagac gctggaaaat ttcagtgaaa agctggaggt cgaagggcgc agcttgtggc
     3121 aggacgcacg tcgacgtttt atgcataacc gtgcggcggt tgccagtctg atagtgctgg
     3181 tgctgatcgc gttatttgta atcctggcac cgatgctttc ccagtttgcc tatgacgata
     3241 ctgactgggc gatgatgtcc agcgccccgg atatggagtc cggtcactac tttggtactg
     3301 actcatccgg tcgcgacctg cttgtgcgcg ttgcgattgg cgggcgtatc tcactcatgg
     3361 tcggtgttgc tgcggcactg gtggcagtgg tcgtggggac actttatggt tcgctttccg
     3421 gttatctggg cggtaaagtg gattcggtaa tgatgcgtct gctggaaatc ctcaactcct
     3481 tcccattcat gttcttcgtc attttgctgg tgaccttttt cggccaaaac atcctgctga
     3541 ttttcgtggc gattggcatg gtttcctggc tggatatggc tcgtattgtg cgtgggcaaa
     3601 ccctgagtct gaagcgcaaa gagtttattg aggcggcaca agttggcggt gtatcgacgc
     3661 cgggcattgt tattcgccac attgtgccga acgtactcgg tgtggtggtg gtctacgcat
     3721 cgctactggt gcccagcatg atcctctttg aatctttcct tagcttcctg gggttgggta
     3781 cgcaagagcc gttaagcagc tggggggcat tgctgagtga tggcgcgaac tcgatggaag
     3841 tctctccatg gttactgttg ttcccagcgg gattccttgt ggtgacactg ttttgtttca
     3901 actttatcgg cgatggcttg cgtgatgccc tcgacccgaa agatcgttaa ggagtgcagc
     3961 catgagcgta attgaaactg caactgtgcc gctcgcacaa caacaggctg acgcactgct
     4021 gaacgtgaaa gatttgcgtg tcacctttag taccccggac ggcgacgtca cggcggtcaa
     4081 tgatttaaat ttttccctac gtgccggaga aacgctgggc attgtaggtg agtctggttc
     4141 gggtaaatcg caaactgcat ttgcgttgat gggcctgttg gctgccaacg gacgcattgg
     4201 cggatcggca accttcaatg ggcgtgaaat cctcaatttg ccagagcgtg aactcaataa
     4261 actgcgcgct gaacaaatct caatgatttt tcaggaccca atgacttcgt tgaatcccta
     4321 tatgcgcgtc ggtgagcagt tgatggaagt gctgatgctg cataagaaca tgagcaaagc
     4381 tgaggcgttt gaagagtcgg tgcggatgct cgatgcggta aaaatgccgg aagcacgtaa
     4441 acgcatgaaa atgtacccgc acgaattttc tggcggcatg cgtcagcgag tcatgattgc
     4501 gatggcattg ctatgtcgac ctaagttgct gattgcggat gaacccacta cagcgctgga
     4561 cgtcaccgta caggcgcaga tcatgacgct attgaatgaa ctgaagcggg aatttaatac
     4621 tgccatcatt atgatcaccc acgatcttgg cgtggtggcg gggatctgtg acaaagtgct
     4681 ggtaatgtac gccgggcgca cgatggaata tggcaatgcg cgcgatgtct tttatcaacc
     4741 cgttcatcct tattctatcg gtttgctcaa cgcggtgccg cgtcttgatg cggaaggtga
     4801 aacaatgttg accatcccgg gtaatccgcc aaacttgctg cgattaccga aaggttgccc
     4861 gttccagcca cgttgtccac atgcgatgga aatttgtagt agcgcaccgc cgctggaaga
     4921 gtttacgcct ggccgtctgc gtgcttgctt taaaccggtg gaggaactgt tatgaatgct
     4981 gtaactgaag gaagaaaagt cctccttgaa atcgccgatc ttaaagtgca ctttgaaatc
     5041 aaagatggca aacagtggtt ctggcaaccg ccgaaaacgc tcaaagccgt cgatggtgtc
     5101 actcttcgcc tgtatgaagg ggaaacatta ggtgtggtag gggaatcggg atgcggtaag
     5161 tccacctttg ctcgcgccat catcggtttg gtcaaggcga ccgacggtca tgttgcctgg
     5221 ttaggtaaag agttgctggg catgaagccc gatgaatggc gtgccgttcg cagtgatatt
     5281 cagatgattt tccaggatcc gttggcatcg ctaaacccgc gtatgaccat cggcgagatc
     5341 atcgctgaac cactgcgtac ttatcatccg aaaatgtcac gccaggaagt tcgcgagcgc
     5401 gtgaaggcga tgatgctgaa agtcgggtta ttgcctaacc tgattaaccg ctatccgcat
     5461 gagttctccg gtgggcagtg ccagcgtatc gggattgctc gtgctcttat tcttgaaccg
     5521 aagctgatta tctgcgatga gccggtttcg gcgctggacg tgtcaattca ggcgcaggtg
     5581 gttaacctgc tccagcagct gcaacgtgag atgggattgt cattaatttt tattgctcat
     5641 gacctggccg tggtaaaaca catttccgat cgtgtgttgg tgatgtatct cggccatgca
     5701 gtagaactgg ggacctatga tgaggtctac cacaatccac tacatcctta caccagggca
     5761 ttgatgtcgg cagtccccat acctgatccg gatctggaga agaacaaaac catccagtta
     5821 ctggaagggg aattaccgtc gccgatcaac ccgccttccg gttgtgtttt ccgtacccgt
     5881 tgcccgattg ccggtccgga gtgcgccaaa acacgtcctg ttctggaggg gagtttcaga
     5941 cacgccgttt cttgcctgaa agtcgatccg ctttaaaaac aataagggct gacagttgtc
     6001 agcccttttt cacgctaaaa gcgattattt attcccgcca gatgatatgg caaagtttgt
     6061 gatctttttc gcggcataac agaatgcggg cgaaaacatc gctgatttca ccatcttcac
     6121 tgtccgccag accaatcacc acttcggcaa aaaagtccgg gttcaaatcg aaatcaacat
     6181 gttcctgcca gtcttccgcc ggatcgaata actccgcgcc gccgcgctct tcaaactgaa
     6241 gattgaacag cagaacatca gctggatcga gattgtcggc agccagttcg agaaaaatat
     6301 cgtaggcctg ctcaagcgtt tcatcttcag tcaggcgatt gtttagatcc atatccatag
     6361 tcactacctg tttaacctct gttggcgacg ttttacagca acgggctgaa gaagtaaaac
     6421 agtcgctcgg cgacacgttg ccataatgga cgttttagcc ataaacgggc atcgagcaga
     6481 cgtgaacgcg aaatataatc gtcctgaacg gcggcaaggt ctgcaccaaa acctttatcg
     6541 tcgattgcca aggtaatctc gaaattcagc cacagactac gcatatcaag gttaacggtg
     6601 ccaaccagac ttagttcgcc atcgaccagc acgctcttgg tatgcagtaa cccgccttca
     6661 aactgataaa ttttaacccc agcagcaagc agttccgtaa agaatgcgcg actggcccag
     6721 ccgaccagca tcgagtcatt ttttcgcgga aggataatac tgacatccac cccgcgctgc
     6781 gccgccgtgc aaatcgcatg aagtaaatca tcgcttggca caaagtaggg cgtggtcatg
     6841 atcaaatatt catgcgccga ataagccgca gtcaataatg cctggtgaat gagatcttcc
     6901 ggaaagccgg ggccagaagc aattgtgtga atggtgtgac cgctggcctg ttcaaacggc
     6961 ataatattga catctggtgg tggcggcaga atacgttttc cggtttcaat ctcccagtcg
     7021 caggaataaa taatccccat cgcggtggcg atggggcctt ccatacgcgc catcagatca
     7081 atccattgcc ctacgcccgc atcttgtttg aagtagcgag gatcgaccat attcatgctg
     7141 ccggtgtacg cgatgtaatt atcgatcatg atcatcttgc gatgttggcg caggtccata
     7201 cggcgtaaaa acacacgcat cagattgacc tttaaggctt cgaccacttc aataccggca
     7261 ttacgcatta gctcgggcca cgggctgcgg aaaaaagcca cactcccggc ggagtcgagc
     7321 atcaatcggc aatgaatacc acgccgtgca gccgccatta atgattcagc cacctggtcc
     7381 gccatgccgc cgggctgcca gatataaaac accatctcaa tattatggcg cgcgagctgg
     7441 atgtcgcgga ttaacgcctg catcacatca tctgactcgg tcatcagctg tagctgattc
     7501 cctttgaccc cagcgatccc ctgacgacgc tcgcaaagct tgaataatgg cgcagcgaca
     7561 ctgctatttt cttcggcgaa gatatgctta caggctttaa ggtcgttaag ccattttgcg
     7621 gtggaaggcc acatcgctct ggcgcgctca gcgcggcgtt tgcctaaatg gagctcgcca
     7681 acggcaagat aggcaataat tccgactaac ggcagaatgt aaataatcaa cagccaggcc
     7741 atcgcggagg gaactgcgcg tcgtttcatt agaatgcgta aagttacgcc tgcaatgagc
     7801 aaccagtatc ccagaatggc caaccaactc accaacgtat aaacggttgt catagattag
     7861 aaatcctttt gaaagcgcat tgttatgagt ttacgcatat ggattcatct ggcaaataaa
     7921 aacgcgggaa aagcactggt cagcgtcggg ggcgggggta taatgaccat tctgttattg
     7981 catagagtag ttaacatgaa gcggagtaga acggaagtgg ggcgctggcg catgcagcgt
     8041 caggctagcc gacgtaaatc gcgttggctt gaggggcaat cgcgccgaaa tatgcgtatc
     8101 cacagcatca ggaagtgcat tctaaacaaa cagcgtaact cgttattgtt tgcgatctac
     8161 aatatctaaa tgtactgggc accattgcgg tgcccaataa gtttactctt ccgtctttaa
     8221 actctatttt tgcgccgatt ctttactgtc agctttcgtt tcttgttcat cgttatcgct
     8281 gaaaatacca tgacctgagt tcaacaacat tgaaacgagc atatcattat taatctcttc
     8341 accatttaaa actcgcgcca gaatttcgct gccaaacagt tgcggtgaga gaatgatatc
     8401 cggatgtacc atcttaatct tattcaggtt tttgctatca ctgacggcga gaactgtttt
     8461 gacatcactg ctcatatctt ttgccgagag tacaacaaac gcgttatctg catcgttatc
     8521 actcagcgcc agaatggccc ggcaacgatc gattcccgct ttctttaata ctgaactgtc
     8581 attactgtca ccggggataa catcagcgtt atcgcctaag cgttgctcaa gttgcttgat
     8641 atcatcttca ggcaagttgc tgataaccgt tacgttttgt ccgcgttgat tcagttgcag
     8701 gatcgtattg atggcgagaa tcgaatgtcc gcaaacaata aaatgatctt tacgatgcat
     8761 tgtatgattg tttcctttta aaagtttgtt gaatcccccg cggataagcg gaccaaaaat
     8821 tgaggtcata gatgtggcaa aaacggtaat gccggaaata atgaccgaaa tagtgaacaa
     8881 tcgtgctgat tcagaaacag ggacaatatc tccatagccg acggttgaca tggtttctat
     8941 cgaaaaatag aacgcggtca tcaaactttc tattcgcgga ttaaaacctt cgcttaaata
     9001 aagcgcaccg taggttgagt aaaacagtaa tgtcgtgaaa ctaataaatg caaaaattgt
     9061 cccggctgcg gcgctactgt gggagaagtc tttgcgcagt atcagcaaaa acaccagcgt
     9121 aaaaatgcaa aatccaatac taaatttcag ccagggataa aaatgcaggg tgtaaattag
     9181 cgctatcaac aacaaaatga tactgattgc ccatgcgagc tttgcccgga acagtaagcc
     9241 aatggagttc aggacaagaa agacgccgag cataaatagt ggggcgtttg ccagcaagga
     9301 gagatccaac tctgaaaatg ctttgatatg gaaaatatca agcaaattga ccgacatacc
     9361 atagattgtt ttaaaaataa gcaatccatt taaaaagacg gccagcgcga gaatgtcgtg
     9421 ccgtagggtc acccataaat ttgttgctgt ttgtttgaat gtagcccagt gactcactga
     9481 tacctctgta accacaattt tgtttaatta tcttagcgtg cacattttct gaacatacat
     9541 gcagcgtgtg aatgtgttaa acaatagtag atgactttat ccgcagtaat caaaggtttt
     9601 gagtgagcaa ctctgcgtca ggaaagggct tttttcctga acatgctcac atcatataaa
     9661 gaaatacaga taaagttatt atctgcttgt ggtagtgaat gcactgaccg gctataagga
     9721 aaggccaaac aagaacacgg ttgcaaaaac cgtgccctta aata
//
LOCUS       DAJTRD010000125         9685 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTRD010000125 DAJTRD010000000
VERSION     DAJTRD010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.125"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(26..214)
                     /locus_tag="OO315_003552"
     CDS             complement(26..214)
                     /locus_tag="OO315_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5892321.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO315_003553"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO315_003553"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5892322.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="OO315_003554"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="OO315_003554"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5892323.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="OO315_003555"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="OO315_003555"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5892324.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="OO315_003556"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="OO315_003556"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5892325.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="OO315_003557"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="OO315_003557"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5892326.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="OO315_003558"
     CDS             5109..6242
                     /locus_tag="OO315_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5892327.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="OO315_003559"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="OO315_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5892328.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="OO315_003560"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="OO315_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5892329.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="OO315_003561"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="OO315_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5892330.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="OO315_003562"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="OO315_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5892331.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="OO315_003563"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="OO315_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5892332.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..9424
                     /gene="epmB"
                     /locus_tag="OO315_003564"
     CDS             8396..9424
                     /gene="epmB"
                     /locus_tag="OO315_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5892333.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         2465 a         2481 c         2466 g         2273 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg gatctccagc tacgccagca
     9421 gtaactgatt aaacagggaa gttaaacatt aaaattgcct gatgcgctac gcttatcagg
     9481 cctacatcgt gcattgaaat tgaatggatt tgcaatgaac tgtaggccgg ataaggcgtt
     9541 tacgccgcat ccggcaagag caacaaacgc aagtacagca accactgaga aatttaatct
     9601 ttgcacgttg cataaccttt aattattcat atgcattgct gttgttactt tttatatagc
     9661 taacaccaat aattaacctt tcttt
//
LOCUS       DAJTRD010000126         9595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTRD010000126 DAJTRD010000000
VERSION     DAJTRD010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.126"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(6..326)
                     /gene="psiF"
                     /locus_tag="OO315_003565"
     CDS             complement(6..326)
                     /gene="psiF"
                     /locus_tag="OO315_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5892334.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(445..1860)
                     /gene="phoA"
                     /locus_tag="OO315_003566"
     CDS             complement(445..1860)
                     /gene="phoA"
                     /locus_tag="OO315_003566"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5892335.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO315_003567"
     CDS             complement(1961..2221)
                     /gene="iraP"
                     /locus_tag="OO315_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5892336.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2403..2606)
                     /locus_tag="OO315_003568"
     CDS             complement(2403..2606)
                     /locus_tag="OO315_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892337.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2684..3778
                     /gene="ddlA"
                     /locus_tag="OO315_003569"
     CDS             2684..3778
                     /gene="ddlA"
                     /locus_tag="OO315_003569"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5892338.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3802..4014)
                     /locus_tag="OO315_003570"
     CDS             complement(3802..4014)
                     /locus_tag="OO315_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5892339.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4274..4582
                     /locus_tag="OO315_003571"
     CDS             4274..4582
                     /locus_tag="OO315_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5892340.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO315_003572"
     CDS             complement(4641..5735)
                     /gene="yaiW"
                     /locus_tag="OO315_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5892341.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO315_003573"
     CDS             complement(5748..6968)
                     /gene="sbmA"
                     /locus_tag="OO315_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5892342.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7320..8477
                     /gene="ampH"
                     /locus_tag="OO315_003574"
     CDS             7320..8477
                     /gene="ampH"
                     /locus_tag="OO315_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5892343.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8478..8981)
                     /locus_tag="OO315_003575"
     CDS             complement(8478..8981)
                     /locus_tag="OO315_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5892344.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9069..>9595)
                     /locus_tag="OO315_003576"
     CDS             complement(9069..>9595)
                     /locus_tag="OO315_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5892345.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2381 a         2448 c         2285 g         2481 t
ORIGIN      
        1 aggtatcagg cggctttctt gaggcaggca ctcataaact tattacgatc atcacctttc
       61 agagcttgtt gcgttgcttg attattacat tcacgcatct tttgctgctg tggcgtcaaa
      121 cttttttcac caggcgcaga cttgctgttc ttcaggcaat cactcatgta ggtcttacga
      181 gcatcccctt tcaacgcctg cgccgtcgcc tgctgattac aggaggtcat acgctgttgt
      241 tgtggggtta aggttctctc ggcagcgccg acggtggtta aaaaaaccag accaaaaagc
      301 aaggtaacca gtaatgttat tttcatagca ccatccctct tcatgtttta accatgagcg
      361 tttgcgcccg tgatctgcca ttaagtctgg ttgctaacag caaaaaaacc acccggcagc
      421 gaaaattcac tgccgggcgc agttttattt cagccccaga gcggctttca tggtgtagaa
      481 gagatcggtc tggtcggtca gtccaacaac attggcggca tgcgggccat acgccgcaat
      541 acgcaactga ctgccggtat gttcttgtga atcctcttcg gaattcccgt aactcatcac
      601 catcactgcg ccatctttag tatttagcgc ctgagtgagg cccggcgctt tggtatccgg
      661 cgcaacaacc tggctggcgt gggcgtgatc agcggtgact atgaccagcg tgttaccctc
      721 ctttttagcg aattccagcg cccgttgtac ggcttcatcg agatcgaccg tctcgccaat
      781 ttgcccacaa gggttcgcag cgtgatcctg tttatcgatt gacgcacctt caacttgcag
      841 gaaaaatcct ttctcatttt tactcaacaa ttcaatggct ttgtcggtca tctgtgccag
      901 ggttggtaca ctgtcattac gttgcggatt aggcgtatag gtgactgcgg gcttatcgat
      961 attgccgtgg tacgttgctt tcggccctag ccagcgcact ggcatattgc cgtcagcaaa
     1021 caggccaagc aggggttttt gctgattcgc ttccgtcacc gaattcagtg aggcagcatc
     1081 gttcaccaac tgataaccac gcgcctgtgc ctgttcacgc agcgtttttc cctgccattc
     1141 accagcggtt gccgtttcag caaaggtttt tgcgccgccg ccaagcgtaa cgtcggcacg
     1201 agcgttaagc agctgttcgg taatcgatcc ttttccgcct ttttccagag cgttacccgg
     1261 acatttttca ctggtcgcgc tcggaccgta gcatttgcgc gaagtcacat gtgccaccag
     1321 cgcagcgggc gtggcatcct gcaactctgc ggtagaaacg ttaccggtcg ccagacctgc
     1381 ggcttttgcc atttccagaa tcgttgggtg atctttttcg tgaatatcga cgcccagcgc
     1441 gccgttatag gttttgacat cggttgacca ggcggttgct gatgcagccg agtcggtgac
     1501 gtagtccggt ttgccagttt ttttattcag cgcatagtga gtgtattgcc cggtaagcgg
     1561 taaggcatcg atacctttaa aaaagccgcc cgcaccttcg gcataattac gtgcggcagt
     1621 aatttccgaa tcccccatcc catcgccaat cagcaaaata atattttttg caggtttatc
     1681 gctaagagaa tcacgcagag cggcggtctg atcacccgtt aagcggcgag caccgccggg
     1741 tgcagtaata tcgccctgag cagcccggtt ttccagaaca ggcatttctg gtgtccgggc
     1801 ttttgtcaca ggggtaaaca gtaacggtaa gagtgccagt gcaatagtgc tttgtttcac
     1861 tttattttct ccatgtacaa atacattaaa aaataaaaac aaagcgacta taagtctcgg
     1921 ccgtgacaac tttatgacag ctgttgaaaa gattaacttt ttactgacaa ggatgcttca
     1981 ataacttctt tacgtaatcg cgcagcagct ctgtatcgtc gtcaggaatg ctggcatcgg
     2041 gctttacctc gtacagcgcc ccctctacct gatcaatcaa ccgctgttgg tcattttgcg
     2101 ccatattgcg aagcattgca gtgacgataa tctccaaagc ttctacctgc gcacacagtt
     2161 ctttcgactc ttcttctttt tgggcaagct taaataacaa ctcagcaatg agatttttca
     2221 tgtctgtatt tccttatcca aagtatggag aagttaacat tatgtttcac aacttcatag
     2281 tgtctggaag tattattttt atgacaaagg aaatactttg cgcaaatatt ttttgtttga
     2341 ttgccagcga aacgtttctc ttatttaaat aattacccac acaaaatata ggcttttaat
     2401 tatcaacctt tattataaat aaaagctatg aaatatattc tcttcgttaa cttataatta
     2461 agagaaaaaa actcaccgca ttgccattta agccaggaat aaattagctt cccgttcatc
     2521 atttttgtga tcttgcgcac atctttgaga taacgcactt tcttcaccag tacgttgcac
     2581 gctttgtcag cagaagattg caacaatctt tcagaaggcc gcgaatcccc cgttttgtcg
     2641 tggtaatgta tgcactttgt gttagacggg ataggttttt aagatggaaa aactgcgggt
     2701 aggaatcgtt tttggtggta aatcagcgga acatgaagtg tctctgcaat cggcaaaaaa
     2761 cattgtcgat gccattgata aaagtcgctt cgacgttgtg ctgctgggca ttgataaaca
     2821 agggcaatgg cacgtcagcg atgccagcaa ttatctgcta aatgcagacg atcctgccca
     2881 tattgcgttg cgcccttcgg cgaccagcct tgcgcaggtg ccaggtaaac atgagcatca
     2941 gcttatcgac gcgcaaaacg gtcagccgtt gccgacggtg gatgtcattt tcccgattgt
     3001 ccacggtacg ctgggcgaag atggttcctt gcagggaatg ctgcgggtcg ccaatttacc
     3061 gtttgtaggt tctgatgttc tggcttcagc agcctgtatg gataaagatg tcaccaaacg
     3121 tctgctacgc gatgccgggc tgaacattgc gccatttatt accctgacgc gcgctaatcg
     3181 tcacaacatc agttttgccg aagtggagtc taaactgggg ttaccactgt ttgtaaaacc
     3241 ggctaatcag ggctcttctg ttggtgtcag caaagtaacc agtgaagaac agtacgcaat
     3301 tgccgtcgat ctggcgttcg agttcgatca taaagtgatc gttgagcaag ggatcaaagg
     3361 tcgtgagatc gaatgcgcag ttctgggcaa cgacaatccg caagccagca cctgtggcga
     3421 gatcgtactc accagcgatt tctatgccta cgacaccaag tacattgacg aagatggcgc
     3481 gaaagtggta gttccggcag ccattgcacc agagatcaac gataagatcc gggcgattgc
     3541 cgttcaggct tatcagacgt tgggatgcgc aggcatggcg cgtgtagacg tgtttttaac
     3601 cccagagaac gaagtggtga tcaacgagat caacacactg cctggcttca ccaacatcag
     3661 tatgtatccg aagctgtggc aagccagcgg tctgggttac accgatctga tcacacgttt
     3721 gattgagctg gcgctggagc gtcacgctgc ggataacgca ttgaaaacca caatgtaata
     3781 ttttaacgcc cgataatatt cttattgggc gttttcagtt tcttcatcgc gacgacgaat
     3841 aatcaacccc gccagccaga aactaatcac ccacgtcacc agaccgacgg cataggtctg
     3901 ccaacctttt gcttcaaatc ccagtacccc caccacgcca ttaagaatga atataaggcc
     3961 aatggcgaac gcatagtagt gccagtcacg gcggattttt acaggcagat tcatggcagc
     4021 tccgttaacg aaaaacaaca tccttgcata ttctgtaaca accgtctgtg gtgtagacaa
     4081 cattttcgta attattaata aatttaactt aaggttgcca ttttgtgact cacaacgaag
     4141 attcacgctt cgggaatatt cccaacatca atttaccatg attctgatat tgacaatcta
     4201 aatgacctgt cagacttgcc ataagccgct ggtatcacac cagaagagaa gcagatgact
     4261 tccggaggtt tttatggctg atttcaccct gtcaaaatcg ctgtttagcg gaaaatatcg
     4321 caatgcctct tcaacgcctg gcaacattgc ctatgcgttg tttgtgctgt tttgcttttg
     4381 ggctggggcg caattgctga acctgttagt gcatgcgccc ggcgtctatg agcgtttaat
     4441 gcaggtccag gaaacaggtc gcccacgggt ggaaattggt ttaggtgtcg gcaccatttt
     4501 cgggctgatc ccgtttttag caggctgcct catttttgca gtggtggcgc tatggctgca
     4561 ctggcgacat cgccgccagt aaaatttacg ttgccagatg cggcctgaaa cgccttatct
     4621 ggcctgacat tcctgtttga tcactgtttc atacaacgcg cccgccgttc gtctacgcgc
     4681 ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg ggctttccag ttgaatgcca
     4741 ggcaacattt cgcggggtag tgatttaccc gttttcgctt cggcaagttg ataaactttc
     4801 ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt tctctaactg acggcgaatt
     4861 tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg ttgccagttc ggttttcccg
     4921 ggttctttac tgtcgtaacg aattaaatca ccgtccagtg ccagcttcac gccgctggcc
     4981 ttactgactg cgttctgaaa cgcggcatta cggctggcgt accagccagc gttaaaatca
     5041 gcaaagcggt atatcggtgc gctatagctg gcgggataat tcagtaaatg gtaagtacca
     5101 aaccacaacc cgccgcggcg actgaagact tcctgacgaa ctgtaccgtc cattttccac
     5161 ggataccctt tggtatgctg ttcggcaaaa gcaatgctga cctgcatcgg cccaccggtg
     5221 cgcaccggat taagcgaacc aaacagcgtc tgccccattg gtaccatgct gatcagatcg
     5281 tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat ctaaccgttc gctataactt
     5341 ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca gaaaagcagg aatgtgcatc
     5401 cgttcagcac gacggtcaat ctcttgccag gcgatcttgc ttaagcccgg aacggccgga
     5461 tctgcctgat aatttgactc ctgctgcgcc accgccagca ccgagtagac gttttccagc
     5521 gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat ctttcgccca ggcatcgcgg
     5581 tctttcacgc tcgcaggcat cttctgccgc acgacgctcg ctacatctat cgctttttcg
     5641 ccctttttca acggttgcgg agcctgacta ctacaccccg ctaaaaccag aaccgccaac
     5701 aatgacagcg aagatagatg attgacgcgc gacattactt ctccttttta gctcaaggta
     5761 tgggttactt cctgaatttt gtcaccatcc agctcatgtt caaagctgcg cagacgtttg
     5821 tagatagaca tcaactcaac cagtgtggtc catgagttaa tcaggtactg gaaagcaccg
     5881 cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc cgagcgtaat cgtaccggca
     5941 acaattgacg gaaacagcaa gaacaaaccg aaaacgttat cgacctgcaa atagagaatg
     6001 cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa aatagttttt ccgtacggcg
     6061 ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg catcgtcttc accataaacc
     6121 agctctttac ggtaggcggc ctctactcgc tggtttttaa actccagccc cggcagtttg
     6181 atccctacca ctgccagcaa tccggtcccc atcagcgacc agacgatagc ggcaatcacc
     6241 agaccatacg gaatgtgccc gataatcggc agctccggca catgcgcgga gagcgttacc
     6301 agcaccggca ggaaggcgat caacgtcatg atggcgttga taaaactgac gcccatattc
     6361 tccagcgttg aagcaaaacg catggtgtct tcctgcacac gctgtgcggc accttcgata
     6421 tgacgcagtt gttgccagtt cgccatgtaa tattcgttca tcgccgtacg ccagcggaac
     6481 acgtagtgac tgacaaagaa gttgttcagt acactgatca ccaccgcgat cagcgcaatc
     6541 cccagaaaga cgcccacttc gcggtaaaat tgttcgatag tgactttatg cggcgaactt
     6601 agcgcggttt gaatcagatc atagaacggc gcataccagg cgttgacggc gaccccgact
     6661 tccaccaaaa accaagtgac gaagataatc agcgcagtac cgagtattga ccagtattgc
     6721 caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa gtcctacgca aacaatgtag
     6781 taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg cggcgctaat cgggatctgc
     6841 ccggaagcgc cggtgatacg tgccacccag tcacccccac cggcttgcca gaagataacg
     6901 gcaatcaatg cccaaacaaa ggccgagaga aaaaacgttc ccggctttgg gaaaaaagac
     6961 ttaaacatat gcactcctgc taacttctta tcgttttaac aactgctgtg tattaaccgc
     7021 atgaccgcta caaagcgtca gccaaatgaa accgcccatt gtgacccata gcaaggcgct
     7081 tagttcgcca gcctgaccat aagaattgtt tcgacggatt tcacaaacca aagattgttt
     7141 acataagcgg attaacacgc gcaggatcac aaatgcaggc attggtgagc aggaaaaaga
     7201 tcgtgtttac gcgccgttac gtttttaaca ctattattgc actaattcgc cctttgcaat
     7261 ccatcaatga gtagtataaa tacgctcagt taccttcatt caatctatgg acaccaccgt
     7321 tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc gtcattgacc tccgtccatg
     7381 cggcgcagcc gatcactgaa ccggagtttg cctctgatat tgtcgatcgt tatgccgatc
     7441 atatttttta cggcagcggt gccacgggga tggcgctggt tgttatcgac ggtaaccagc
     7501 gcgtctttcg tagttatggt gaaacgcgac ctggtaataa cgttcgcccg cagctggatt
     7561 cggtcgtgcg tattgcttcc ctcaccaaac taatgaccag tgaaatgctg gtgaaattgc
     7621 tcgaccaggg gaccgtaaag ttaaacgatc cgctaagtaa atatgctccg ccaggcgcac
     7681 gcgtgccgac ctacaatggg acgcccatta ccctagtgaa tctggcaacc cataccagcg
     7741 ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc ggtgtttgtc tggccaacgc
     7801 gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa agccgcgcca ggcagtcagg
     7861 cggcgtactc taaccttgcg ttcgacctgc tggcagatgc actggcgaat gcgtccggca
     7921 agccttatac ccagttgttt gaagagcaga ttacccatcc attggggatg aaagacacca
     7981 cctacacccc ctcaccggat cagtgccgcc gtttaatggt tgctgagcgc ggtgccagtc
     8041 cgtgcaataa cacgctggcg gcaattggca gcggcggcgt ctattccacg cctggcgata
     8101 tgatgcgctg gatgcagcag tatttgtcat ctgattttta tcagcgcagt aaccaggcag
     8161 atcgcatgca gacgttgatt tatcagcgcg cgcagtttac gaaagtgatt ggcatggatg
     8221 tccccggcaa agcggatgcg cttggtttag gctgggtata tatggccccg aaagagggtc
     8281 gtccgaggat tattcagaag acaggcggtg gtggcggttt cattacctat atggcgatga
     8341 tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg ttcgccgttg acgcgcttta
     8401 aaaatatgag cgatggcatc aatgatctgg tgaccgagct aagcgggaat aaaccgttgg
     8461 ttatccccgc atcctgatta atactctgaa ggcaaacggt tgatagcgac cagtttgcct
     8521 ttattcattt cgatatagcc gcctttacgc aaagcagcga gaacttctgc gacgacagaa
     8581 cgcgatatgc gtgtacgttg atggatataa ttcatcacac caatacgtga tcgcaattct
     8641 tcattccagt caatcattga taataatgtt gcgcgggttt gtttggctgg tagatgatat
     8701 cccgtacaat ttccttctga tattaatttg tatggtatat cgtttttcat taaaccatca
     8761 gccagcccca taatataagg agcctgggta ataccgataa gtacgttttc ttctctacgc
     8821 agagagataa ctccttctaa aataacgaaa gtatcttcgt tattgacatc actggaaaat
     8881 ataacttgct tttcattatt aaactcgaag cgcgtaccgt atctggacaa acatttatcg
     8941 agcttaccaa attcctgaag aggtttaact acagataaca tttgcgcgtc ctttgcagta
     9001 atgcccgtca aatccttgac gggcattatt tagattaaat taccagtatt tcttcggagt
     9061 gaagaatatt accaagtata tttaacaccc acgttcgcgg accagtcttg atctacgtca
     9121 ccaccaccga ggtagttagc atcggtatag gcgctgaagt tcttggtgaa gctgaactga
     9181 gtacccagac caacacgtac cgcagaccct tcagtaccgt tatcgatgga atcaccgttc
     9241 acatcgttat cgttgttaga gtcgtcgtag acgtaagcca gtttgaagta cggagtcaga
     9301 gcctgatctt cgctgtaggt gaaggtataa cctgcatcta cacccagttc ataacgcatg
     9361 ctgtcgtaag gctgaccgtc aactttcatg tcgttgctca gctggtagtc atcaccagac
     9421 tggaacagac cagaaatgct gccgtaagga gtcacgtaac cagcatcacc cagtttgaag
     9481 tcgtaaccgg ttttcaaacc gaagccccaa gcgtcggagt tggtgctacc gtcaacgtaa
     9541 gtaccgttgc tcatggttgc agacaggtcg ttgttgaagt gagagtagct caagc
//
LOCUS       DAJTRD010000127         9523 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTRD010000127 DAJTRD010000000
VERSION     DAJTRD010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.127"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..594)
                     /locus_tag="OO315_003577"
     CDS             complement(<1..594)
                     /locus_tag="OO315_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR5892346.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQN"
     gene            complement(661..1386)
                     /gene="fimC"
                     /locus_tag="OO315_003578"
     CDS             complement(661..1386)
                     /gene="fimC"
                     /locus_tag="OO315_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5892347.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1423..1962)
                     /locus_tag="OO315_003579"
     CDS             complement(1423..1962)
                     /locus_tag="OO315_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5892348.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2027..2575)
                     /gene="fimA"
                     /locus_tag="OO315_003580"
     CDS             complement(2027..2575)
                     /gene="fimA"
                     /locus_tag="OO315_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5892349.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3056..3652)
                     /gene="fimE"
                     /locus_tag="OO315_003581"
     CDS             complement(3056..3652)
                     /gene="fimE"
                     /locus_tag="OO315_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5892350.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(4130..4732)
                     /gene="fimB"
                     /locus_tag="OO315_003582"
                     /pseudo
     CDS             complement(4130..4732)
                     /gene="fimB"
                     /locus_tag="OO315_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6186..6902
                     /gene="nanC"
                     /locus_tag="OO315_003583"
     CDS             6186..6902
                     /gene="nanC"
                     /locus_tag="OO315_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5892351.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6922..8028
                     /gene="nanM"
                     /locus_tag="OO315_003584"
     CDS             6922..8028
                     /gene="nanM"
                     /locus_tag="OO315_003584"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5892352.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8093..9073
                     /locus_tag="OO315_003585"
     CDS             8093..9073
                     /locus_tag="OO315_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5892353.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2788 a         2076 c         1996 g         2663 t
ORIGIN      
        1 attctgtaca ctatttccgc tgaaattata attgagcaat ccggcattaa taccgggatc
       61 ccataactca ggaggaatat aaccacgcgc gcgattactc ataaatgcct gagggatcgt
      121 caggttcagt cgctgctgac caacatccag atgcgcagta gcgtcctgga ccattgtggt
      181 taatggcaca caggcatcat ccgccagcag attcataccg gcgacagaag ccgtattcag
      241 ccccatactg gcgagttgcg cgcgtgtcag gcagggaaca atcccttgtt cactgtcgcc
      301 cgtattaaat gtgacatcac gcgttgccat ataaccatta ttcaaataga tatcgacgcg
      361 atacgtccct ggcggtaatt cttgcccatt ttcaaaacgc gataaatcgg ccacagcctg
      421 gggatcatcc gctaaaaagc gcagattaaa atagaggtcg gcagatgaca aaggtgcctg
      481 tgcggcaaaa gcacaggcga caacgagtcg gacaaaaaaa ccagccaaac gatgcttacg
      541 aatatgcaag cattgtgtgt ttcgctggta aagtcttaaa ttcagatatg acattattcc
      601 tccggtttaa aatcacccgg cagtcaattc tttttattca ggcgaaaaat tccccctgcg
      661 ttattccatt acgcccgtca ttttgggggt aagtgcgcca taatcattta ttgttcggta
      721 agtaatattg cttcctgcat cagaaggcaa tttaaccgtg ctttcgccca ttggaggcac
      781 caatgcattt tcaagaaccc gggttccggc attcaactct gttaccgtca ggtaataggg
      841 tgtcgggtta atcagcgtca gagaattcgc gctacgacga aatcttaatt tttctgcggc
      901 ctgatcgggt ggcaacgcta atttagccgg gcgatagtac agtttaatgc ggctgataat
      961 tgcgagctgt agcatattct cagtcaattt tgatttatcc attgacggaa tcgctttaac
     1021 gttcatccag aataaacttt cccggtcctg tggcaattgg ttatttgttg catcaagaat
     1081 acgtaaggta ttctcttttt ttcccttcat cgcaaacaga ggaggcgtca cgataaaacg
     1141 accatccttt acaccatcgg cattttccac ccatgattga attaaatagg tactattttc
     1201 atcattattt gtcacggcaa gttgcacttg tttttgccct gccggataaa ttacgcgagt
     1261 cgcacctaag gccactcctg cttcagcgcg tccggcaacc atcattgcca tgaacatcag
     1321 gatacctgcc agcaagcaga atgttatttc ctgcgatttc cttacattga cgtttttatt
     1381 actcactgtc ctgttcctgt tatcacatta tctactgaac aattactgat aggttaaaga
     1441 gaaccaggcc tgggcattgg cgatgccgcc agtaacccga cgcccggtag cacgatattt
     1501 ggcgatgaaa tgtagcgaag tagagcctga gtaaagccgt ttccagtttg ctggaggacg
     1561 attaatcggt acgaggtttc cttcatcatc aaacaacgct acaccaatat tggaggctat
     1621 ccctggcccc tctcccacgg agagcacatc cggattttta ccatccgcga caccgtgaaa
     1681 cgccacaccc acacgttcac tcaccaccgt gctacattcc cgtaaatgaa taacaaaagg
     1741 caccggtgcg ctatcttccc ctgccgcatg aaaccggtta ctgctgattt gccccatatt
     1801 gaccgtcatt tgtttatcac cggcttcaat ccggcaagtt tccgcaataa tgacgccctg
     1861 aaattgcata tttccgccgg gcagcgtggt attccattta tttccggcca gagcaaacat
     1921 tggcagcaac gacgctaaca gaaataatct cttccttttc atcgttattt ttatcgcaca
     1981 agggtgggca tccctgcccg taatgacgtc cttgaacctg ggtaggttat tgatactgaa
     2041 ccttgaaggt tgcatccgca ttagctgcac ccggggttgc ctcgccgatt gcataataac
     2101 gcgcctggaa cggaatggtg ttggtaccgt tattcagggt tgtttgtgca ctaaatgtcg
     2161 caccgtccag cgtcagagca ttgcctgttc tgtctaggat ctgcacgcca acgtttgttg
     2221 cactacctgc agcagaactc tgcagagcca gtacatcagt acgcgtagca tcaattgccg
     2281 tacctaagaa ggcaacagcg gcttttgtgg caacagtggt atcgcaatca ttcagctgaa
     2341 tgttaaaacc aacggcagag ctggttgctc cagcctgctt caggctagcg gtacgaacct
     2401 gtcccaactg aacggtttga tcaacagagc ctgcatcaac tgcgcaagcg gcgttaacaa
     2461 cttccccttt aaagtgaatg gtcccaccat ttaccgtcgt agtatcggcc agagccgctg
     2521 cggaactgag ggacagagcc gacagaacaa cgattgccag agttttaatt ttcatgctgc
     2581 tttcctttaa aaaaactatt tctaaatcga catgggcagt cgttctgtac actttgtttt
     2641 gtcaacgagt ttgactgcca acactgcaca gttttccccc aaaagatgaa acatttgggg
     2701 ccattttgac tcatagagga aagcatcgcg cacaaacttt ttcagtttat ttgttggctt
     2761 aatattctat tattatcttt atttatagat ttttatattg catgaggtgg tttttggaga
     2821 gaagaatgag gaagatgcgt cgagccacag aaacgttagc tttacatata gcggaggtga
     2881 tgtgaaatta atttacaaga gaaataattt acatatcaaa tagttagatg ctttttgtcg
     2941 ttttttaata tttttatgct tgagaaaaaa tacgtaactt atttatgata tggacagttt
     3001 ggccccaatt gtcttgtgtt tatttgtttt ttaactttat tatcaataag ttaaatcaaa
     3061 cctcttctct ttttaatttt tcgtttatga gattatttct ttcccataat ccggcaaaac
     3121 gagcagcatt actggcggta taacgcacag tatggcgaat atttcgatgc ccgagataat
     3181 cctgaattaa acgggtatct gcaccacgct ccgccagttc ataaccgcaa gcatgcctta
     3241 acatatgagg atgagtctgc gttacggttc cagcttcaat accggcatcg cgaataatgc
     3301 gataggcctg ctggcgagaa agccgactcc cgcggcgaga aataaatata gcgtcggtcc
     3361 ggtcagcgcc tttccagtta gcacgttcct gggtccagcg ttccacggct tcacgctcat
     3421 caaaacgtaa cgggtgaacg gtagaaaatc cgttcttcag tcggcgaata tttattcgac
     3481 cttcattaag gtcaaggtcc tgataatgca gatcaagcag ttcactaata cgcatcccat
     3541 gccgatatgc caacagaata agacaataat ctctggctcc cgttgccccg taacaaaccg
     3601 cctgcatcat ggcctgaact tctttaccgg taagataacg acgtttactc acaatagtag
     3661 tactcctaac tgagatatat ttgaatgaat acctatagga aacctcaatc ggtcaaaatt
     3721 agcccaatag ataaaaagat aaccacacac gaagttatgt ggctatcagt caattacatt
     3781 aaacatcata caaaaataaa atatcactga tgtgacaaca tctattttca ttgatttgat
     3841 tcaatataac aatagattac agaggattaa cttccagtta cttaaaaaat aaagaaaaaa
     3901 gtgtggaaac aggagaattt aagtgatgtg taaacttatg aatgattttg tttatttatt
     3961 ttgaagaaca taaatcatta gattttgtat gggtatggtt aacttaatgt ttattatgat
     4021 aattaagaat cacacttaca ataacatcaa ttaataaatg gttaatcatt tttgatatat
     4081 cgtaagaata atgtagtttt taacaccatc cctggtatct caactatctc tataaaacag
     4141 catgacgctg tcgtcctctg gctctatccc agatgccgta aaaacgccct gcattgctgg
     4201 cggtatacca gacagtatga cgaatattgc gatgcccaag ataatcctag ataagtcgcg
     4261 tatctattcc catattcgcc aaagcaaaac cacacgaata gcgtaacatg tgcggatgaa
     4321 tctccagtga caacccggca ttaccaccag aagtcgagat aatatggtaa aactgttgcc
     4381 gagaaagcgg attcccctta cgtgataaaa atacccactc gctctcagca tgcggataag
     4441 aagtacggat actcaaccag ttttttaaag cctgaacttc tttgttcaat agcgggtgcg
     4501 ttgttgaaaa gccttttttt aatcgatgga tatatataca ctttgcttta agatcaatat
     4561 ccgaaatcct caatcgacaa atttcactcg cccggaaacc atgaataaaa caaagcaaaa
     4621 tcagacaata attacgtgtt gcatgaggcc cggtatttgc tgctttaagg agtgactcga
     4681 tttcactatg ggtaaggaag ttcctttttt tgttatcagc cttattcctc atcgttttcc
     4741 cttataatta cagacgcgca ctagctgtgc tgggttagca ataatccaac atttttatcc
     4801 tgattatgtt tataaaagcg aacgtttgct taataactta cgtaagtgga ccagttcttc
     4861 tgagcgaact taaatggagt agcgatagtt acttataata actttgccat acaaacgcca
     4921 tgctcttgca tgctatgtac ctttatatat ttatcaatct gatacgaatc taataataac
     4981 cctcatcatt aatgcaacta atcttatcta tatatcatgt gatatgttct gtaacaagta
     5041 aaatcaacat aaaaaattca gcaaaattta atatttgtta aactagccgt ctcaaatatg
     5101 acattattag ttaactgcat ttgccacaaa atggctattt catggaacgc agtaatttct
     5161 tagtggctat tatcatgcta attcttagat ccctagcgat tattcctgcc tggttcgtca
     5221 gcttaggcca tgagattgtt acattccttt ttatcctgga gatatggggg aacgcaggcg
     5281 acgctttttc atctacatcc gttggtaaat atagctttgg tcactacgac ttaagccttc
     5341 ctcaatcaca gctattcgcc gggcgatacg gcaaacaaac taagtgaaac atcatcctga
     5401 aaacaccaac atcaacaagc ctctccagat cgacatcagg agtgaccagt tacaagccac
     5461 aaaccaagaa acatatttca tgatgagaat tatgctcagc ctgatggcga gtaaaccctt
     5521 attttcacca ctctcactga gttagcttgc aaagctttct ttgtttagat tctaaataaa
     5581 ttatttataa aacgtatagt taaaagaaaa tctttagttt ttgaagacca ccgcaagtgt
     5641 tcgtctggct tcacatggca tcttcttctt agaaaaagat cgacatattt tgtgacgcga
     5701 attgcaaatc tggttttgtt gtatggattg cgtgattttt gatctggtat aacaggtata
     5761 aagatgcacc acgatagtca atgagactgg gcatctcgta atctatggca aacatcactt
     5821 cagttctttc acatcgggtg atgaaaatgc acttcagtct gaaaggaata tgaaaatgag
     5881 atcaacagac actctatttt atgactctgg gtaaaatggg ttgagtaagt gatataactt
     5941 acgaatattc aaatcaatta aaaatcagaa gagattttat actcaggtat ttaatctgga
     6001 tctctgttta tttaaataat gttaaaagag aattttcaca ggagacctta tacaaaaaat
     6061 ataaaataca gttatccgtt gccaaagaca ctataagcct ggcaaaaaaa tattacacaa
     6121 cataaatgct aattgtttat gcgggctttg tattgctttc tgtatcctac aaatgagtga
     6181 aatttatgaa aaaggctaaa atactttctg gcgtattatt actgtgcttt tcgtccccat
     6241 taatttctca ggctgcgaca ctggacgtac gtggtggata tcgtagtgga agccacgcct
     6301 atgagactcg actcaaagtc agtgagggat ggcaaaatgg atggtgggca agcatggaaa
     6361 gtaatacctg gaataccatt catgataata aaaaggaaaa tgccgcactc aatgatgttc
     6421 aggttgaagt taattacgca attaaacttg atgatcaatg gacggtgcgc ccgggaatgt
     6481 taacgcattt tagcagcaac ggcacacgct acggacccta cgtaaaactg tcctgggacg
     6541 cgacaaaaga tcttaatttt ggcattcgct atcgttacga ctggaaagct taccgacaac
     6601 aagacttatc cggtgatatg tctcgtgata acgttcatcg ttgggatgga tatgtcactt
     6661 accatattaa tagtgatttc accttcgcat ggcaaacgac gctatacagc aaacagaatg
     6721 attatcgcta tgcaaaccac aagaaatggg cgacggaaaa tgcctttgtt ctacaatacc
     6781 atatgacgcc cgatattacg ccatacatag aatatgacta ccttgaccgc cagggtgttt
     6841 acaacggcag agataattta tcggaaaaca gttatcgcat tggtgtgtca tttaaactgt
     6901 agtagacagg agacagtcac aatgaataaa actataatgg cgcttgctat catgatggct
     6961 tcatttgccg caaacgcgtc tgtattaccg gaaactcctg tgccatttaa aagtggtacc
     7021 ggagcaattg ataacgacac tgtctacatt ggtttaggta gcgcaggtac agcatggtac
     7081 aagctagata cacaggccaa agataaaaaa tggactgcgt tagctgcatt ccctggtgga
     7141 ccgagagagc aagcaacctc tgcatttatt gatggcaatc tgtatgtgtt tggcggcatt
     7201 ggcaaaaaca gcgagggatt gactcaggta tttaatgacg tacacaaata caaccccaaa
     7261 accaatagct gggttaaatt gatgtcgcac gcgccgatgg gcatggcggg tcatgtgact
     7321 tttgtacaca acggcaaggc ttatgttact ggcggtgtta accagaatat cttcaatggc
     7381 tattttgaag atctcaacga ggctggaaaa gattcaaccg ctatagataa aatcaacgcc
     7441 cactattttg acaaaaaagc agaagattat ttcttcaata agtttctgtt gtcttttgat
     7501 ccctcaacac agcaatggag ttacgctggc gaatcgccct ggtacggaac ggctggtgcg
     7561 gcggttgtga ataaaggtga taaaacctgg cttattaatg gcgaagccaa accaggattg
     7621 cgaacggatg ccgtatttga acttgatttc accggtaata atttaaaatg gaataagctt
     7681 gatcccgtct catcaccaga tggcgtcgct ggcggttttg cggggataag caatgattct
     7741 cttatatttg ctggaggggc cggattcaaa ggttcacgag aaaattacca gaacggtaag
     7801 aactatgcgc atgaaggcct gaaaaaatca tatagcactg atattcatct ttggcataac
     7861 gggaaatggg ataaatcggg tgaattatcg caaggtcggg cctacggagt atcattgccc
     7921 tggaataata gtttattaat tattggcggt gaaactgcag gcggcaaagc ggtgacggat
     7981 tcagttttaa tttctgtgaa ggataataaa gtcacagtac aaaactaacg cttcagggcc
     8041 ccggtaaagg ggccctgcta tcaatttgtc atttgtatta cgcgaaaatt atatgaacgc
     8101 aataatatca cccgattatt actatgttct taccgttgct ggtcagtcta atgccatggc
     8161 gtatggcgaa ggactgccat taccggacag ggaagatgcg cctcattcca gaattaaaca
     8221 attagcgaga tttgcgcata cgcatcccgg aggcccgcca tgtcacttta acgacattat
     8281 tccactgact cactgcccac acgatgttca ggatatgcag ggttatcacc atcctctggc
     8341 aacgaatcat caaacacagt acggcaccgt tggccaggca ctgcatattg cacggaaatt
     8401 actgcccttc attcctgata atgcagggat tcttatcgtt ccgtgttgcc gtggcggatc
     8461 ggcttttacc gcgggcagcg aagggacata ttcagaacgg cacggagcca gccatgatgc
     8521 ttgtcgttgg ggaacggata ctccgctgta ccaggattta gtcagcagaa cgcgagccgc
     8581 actggcaaaa aatccgcaga acaaattcct cggcgtatgc tggatgcaag gcgaatttga
     8641 cttaatgacc agtgactacg cgtcacaccc tcaacacttt aatcatatgg ttgaagcctt
     8701 tcgtagggat ctaaaacaat accattctca gcttaataat attactgacg cgccgtggtt
     8761 ttgcggcgat accacctggt actggaaaga aaatttcccg cattcgtatg aagctattta
     8821 tggcaattat caaaataatg ttttagccaa tattattttc gtcgacttcc agcaacaagg
     8881 tgaaagagga ctgacgaacg cgcctgatga agacccggac gatttaagca cgggatatta
     8941 cggttcagcg taccggtcac cggagaactg gacgacggca ctgcgaagca gtcatttcag
     9001 cacggcagcc cgtcggggga ttatttctga cagatttgta gaagcaattt tgcagttttg
     9061 gcgcgaaagg tgagcgggga gtttattcat gggggatgtc cttgttcgtt ctcagagtag
     9121 acaacaaaaa acaaatgaga tcaaggtaat atctcttcac ttgcgggctc gaacagcagg
     9181 caattgccaa tataataatc tggcacaagg gtgaaaaacg cgcaccgcat aaaccgttgt
     9241 tattgcttac agattagccg gatgccttaa tagacatccg cgccttttcg attatggctc
     9301 ggaaatctac gaacatctac acagtttact ggaacgtttt gggccacagc gttcgcagca
     9361 ccgtcctgat atgccgttct ggcgattata agtcgacgga ttctggcaaa tagacgatga
     9421 tgatatgctt aatgatgttc aaagaaatta ggccattatt atattattgc ttgcattttc
     9481 tttcattcct aaaagcaccc gccattaatc ccaggcgcat tat
//
LOCUS       DAJTRD010000128         9497 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTRD010000128 DAJTRD010000000
VERSION     DAJTRD010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9497
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.128"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..200)
                     /locus_tag="OO315_003586"
     CDS             complement(<1..200)
                     /locus_tag="OO315_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001391643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892354.1"
                     /translation="MCYEYDAYYLYRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGV
                     VCDPYICVNSDGISPELTRKYLG"
     gene            713..1408
                     /gene="minC"
                     /locus_tag="OO315_003587"
     CDS             713..1408
                     /gene="minC"
                     /locus_tag="OO315_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5892355.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1432..2244
                     /gene="minD"
                     /locus_tag="OO315_003588"
     CDS             1432..2244
                     /gene="minD"
                     /locus_tag="OO315_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5892356.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2248..2514
                     /gene="minE"
                     /locus_tag="OO315_003589"
     CDS             2248..2514
                     /gene="minE"
                     /locus_tag="OO315_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5892357.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2886..3230)
                     /locus_tag="OO315_003590"
                     /pseudo
     CDS             complement(2886..3230)
                     /locus_tag="OO315_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3265..3384)
                     /locus_tag="OO315_003591"
     CDS             complement(3265..3384)
                     /locus_tag="OO315_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5892358.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3345..3530)
                     /locus_tag="OO315_003592"
     CDS             complement(3345..3530)
                     /locus_tag="OO315_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892359.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3631..3804
                     /locus_tag="OO315_003593"
     CDS             3631..3804
                     /locus_tag="OO315_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892360.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3806..4150
                     /locus_tag="OO315_003594"
     CDS             3806..4150
                     /locus_tag="OO315_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5892361.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4154..4489
                     /locus_tag="OO315_003595"
     CDS             4154..4489
                     /locus_tag="OO315_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5892362.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4546..6867)
                     /locus_tag="OO315_003596"
     CDS             complement(4546..6867)
                     /locus_tag="OO315_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5892363.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6984..7193)
                     /locus_tag="OO315_003597"
     CDS             complement(6984..7193)
                     /locus_tag="OO315_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892364.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7944..9467)
                     /locus_tag="OO315_003598"
     CDS             complement(7944..9467)
                     /locus_tag="OO315_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5892365.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2666 a         2205 c         2078 g         2548 t
ORIGIN      
        1 ccgagatatt tccttgttaa ctctggggaa atgccatctg agttcacaca aatatacggg
       61 tcgcaaacaa cgccgcacgt cggagattga agtccgccag cgtcatgtcc agacgccatt
      121 gatgagaaca tcatccccac acctgacaat ccgatataaa taatccgata taaataatac
      181 gcatcatatt catagcacat cctgataatc caaccgtaca acacttatgc tcatttcgtc
      241 atcaatccgt aatcaaaacc gctgacctgg acgagttacg atgacctgac agaagtatca
      301 gacgtcttct gcggtttcta tgatagagca attctgtggc ggatattaag ataacgggta
      361 tctgttcttt aatgatgctg caaaaaatgg aaagttaaga aagcctggcg tggtgaaaaa
      421 atacacttgc ctcaatactg gatatttaaa caggtctgga gatgctgtca cgattagggt
      481 gcggaaaaag gaagcgtaaa taagaagaat caaaagtacg acggcaatgg gttgattgac
      541 aagggtattt tttaagctat gaatcagcgc catttatcac agaatagact tttactctga
      601 ataaatggga gggtgacttg cctcaatata atccagacta taacatgcct tatagtcttc
      661 ggaacatcat cgcgcgctgg cgatgattaa tagctaattg agtaaggcca ggatgtcaaa
      721 cactccaatc gagcttaaag gcagtagctt cactttatct gtggttcatc tgcatgaggc
      781 agaacctaag gttatccatc aggcgctgaa agacaaaatc gctcaggccc ccacattttt
      841 aaaacatgcc cccgttgtac tcaacgtcag tgcactggaa gacccggtaa actggtcagc
      901 gatgcataag gcggtttcgg caaccggttt gcgggttatt ggcgtaagtg gctgcaaaga
      961 tgcgcaactt aaagccgaaa ttgaaaaaat ggggctgcct atcctgacgg aaggaaaaga
     1021 aaaagcgcca cgtccagctc ccgcaccgca ggctccagcg caaaatacaa tgccggtcac
     1081 aaaaacgcgt ttaatagata ccccggtgcg ttccggtcag cgtatttatg ctccacaatg
     1141 tgatctgatt gttacaagcc acgttagcgc tggggccgaa ttgattgccg atgggaacat
     1201 tcatgtctat ggcatgatgc gcggtcgtgc gctggcaggg gcaagtggtg accgggaaac
     1261 gcaaatattt tgtacgaacc tgatggcgga attggtgtcc atcgcaggtg aatactggct
     1321 gagtgatcaa atcccagcag aattttatgg caaagcggcg cgactgcagt tagtcgaaaa
     1381 cgctttgacc gttcaaccgt taaattgatc cctttttaac aaggaatttc tatggcacgc
     1441 attattgttg ttacttcggg taaagggggt gttggtaaga caacctccag cgcggccatc
     1501 gccactggtt tggcccagaa gggaaagaaa actgtcgtga tagattttga tatcggcctg
     1561 cgtaatctcg acctgattat gggttgcgaa cgccgggtcg tttacgattt cgtcaacgtc
     1621 attcagggcg atgcaacgct aaatcaggcg ttaattaaag ataagcgtac tgaaaatctc
     1681 tatattcttc cggcatcgca aacacgcgat aaagatgccc tcacccgtga aggggtcgcc
     1741 aaagttcttg atgatctgaa agcgatggat tttgaattta tcgtttgtga ctccccggca
     1801 gggattgaaa ccggtgcgtt aatggcactc tattttgcag acgaagccat tattaccacc
     1861 aacccggaag tctcctcagt acgcgactct gaccgtattt taggcattct ggcgtcaaaa
     1921 tcacgccgcg cagaaaatgg cgaagagcct attaaagagc acctgctgtt aacgcgctat
     1981 aacccaggcc gcgtaagcag aggtgacatg ctgagcatgg aagatgtgct ggagatcctg
     2041 cgcatcaaac tcgtcggcgt gatcccagag gatcaatcag tattgcgcgc ctctaaccag
     2101 ggtgaaccgg tcattctcga cattaacgcc gatgcgggta aagcctacgc agataccgta
     2161 gaacgtctgt tgggagaaga acgtcctttc cgcttcattg aagaagagaa gaaaggcttc
     2221 ctcaaacgct tgttcggagg ataagttatg gcattactcg atttctttct ctcgcggaag
     2281 aagaacacag ccaacattgc aaaagaacgg ctgcagatta ttgttgctga acgccgtcgc
     2341 agcgatgcag aaccgcatta tctgccgcag ttgcgtaaag atattcttga ggtcatttgt
     2401 aaatacgtac aaattgatcc tgagatggta accgtacagc ttgagcaaaa agatggcgat
     2461 atttctattc ttgagctgaa cgtgacctta ccggaagcag aagagctgaa ataagcccgc
     2521 tgtaaaaacg catttatctt caaggcagag tcatttctgc cttgagtttt tcatccctct
     2581 catccacgtt gtggtaaagc ggcgagtatt cttgctgata ctcctcattg ctatttccac
     2641 ctcccccttt acaccttaag gctgttccca cgcacagaac aaaatgttta tgcctggttg
     2701 agtaaataac catgttatta gtgtgtatat actgggagat gataaccccg caatttcatt
     2761 attcattgtt ctcccatagg atgaggtttc ccgccgtaac tggcgggctt tttttgcccc
     2821 agaattttct ctttcaacat cctgtaaacg aaaactgcgc cgaagcgcag tttaatcgtt
     2881 gatggtcaga acgaccagtt cacaccagcc accgcgttcc acggggactc cacaccggca
     2941 ccatggctat accccacccc gaaatgcccg cttaacgtac tgctgaatga ggctttaata
     3001 cctgcctggt atattccacg tctgcccgac aaatcattga caaaattacc gtcactattc
     3061 actttcaccc gattatcatc gacaaattct ttgcgcacag ccgccttcag gcagggttca
     3121 atttccatac cgttccccag acgcatgttg taactcagcg ttgcacccag ttcacgatat
     3181 atactgcggg tatcgactga tttcgattcc atgccattgg ataaatgata gtagcgtcta
     3241 agtgacctaa gctctgctgc aacatcagcc cgcaggcacc agaccagggg attcatcctg
     3301 aagagacagc gcaggtgtat tgtgttcacc gctcaccaaa gacatcatga tgaaatgatg
     3361 atatcccgca taagaaagag gcatttttta cacgcagtgc gctgaagtgt agttggataa
     3421 aaaatcactc ttttcagata atactgtcgt acgcttatct ttagacaatg aggccgtcgg
     3481 aaaaataaaa tgactaatgt aatcgttgct gatgaatatc ttctgctcac ataaaaatca
     3541 cacaataata ttgataaatc agattatctt acctttacaa cattgcgtta acagtcaatg
     3601 acacttcttg aataaaaata ttgtcaatca atgagttatt cctcatttaa gattattctc
     3661 attcacgtaa aaaacataat tcctatcatc acagccacgc tcattttgaa ttatctgaat
     3721 aatagcgagc gttctttagt taagcagatc ttaattgaag acgaaattat tgtgtgtgca
     3781 acttacttaa taccggacat ctaagttgaa gaaaaaaata cttgccttcg ggcttatctc
     3841 agcattgttc tgctccacgc cagcgatggc ggatatgaac cgcaccacga aaggtgcatt
     3901 gctaggcgca ggcgttggtt tacttactgg caatggtgtt aatggcgtac tgaaaggtgc
     3961 agctgttggc gctggtgtta gtgcagtaac agaaaaaggc cgcgacggta aaaatgcgcg
     4021 taaaggtgca aaagtgggtg ccgccgttgg tgctgtcact ggcgttctga ccggcaatgg
     4081 cctcgaaggg gcgattaaag gtgccgtgat tggcggtacg ggtggtgcaa ttctgggtaa
     4141 aatgaaataa ggaatgatga tgaaaaaatt tgctttactg gcgggcctgt ttgttttcgc
     4201 accaatgacc tgggcgcagg actacaatat taaaaacggt ttgccgtcag aaacctatat
     4261 tacttgcgca gaagccaacg aaatggcaaa aactgacagc gcacaggttg cagaaattgt
     4321 tgcggtaatg ggtaatgcca gcgttgccag ccgtgattta aaaattgagc aatctccgga
     4381 actgagcgcc aaagtggtag aaaaactgaa tcaggtttgc gctaaagacc cgcaaatgct
     4441 gttgatcacg gcaattgatg acaccatgcg tgctatcggc aaaaaataat atttcatatg
     4501 taccgcgtcg atgaatacga cgcggttttt tagctaacgg cgggatcaga acttatattt
     4561 caggcccacc atcactgcgg tgtcattgta gccattatca cccagctgca cgcccacatt
     4621 cccccagacg ctggccgctg gattcaggtt gccatttacg ccaagtttaa tttcccccaa
     4681 attactcacc ccatcttgtt cgactttcac accgttcatt gagacagcaa aatctttact
     4741 gttatggatc cagttcattt caacaaaagg ttcaaagtcg tcaccgtgcg gaccgctgtt
     4801 tttctcctga gtacgaataa aggtgcggaa gccgagtcgg gtctggatgt tgttatttgc
     4861 gtcattctca atacgtgagc cattggcttc cgtgtgcgca ttatgatcga cgcccatcca
     4921 gatagcctgt gcctgtggct ggaagatcca cgtatatttc gccgcttcca gaccaaagga
     4981 tttattcagg gcaaaaccgt aacccgcttc cagcgagacg gttgcacctt tcgcgctata
     5041 ggattcatag gctaattcat cccctttcac ggagttttta aaccagctat attgcgccca
     5101 ggagtcaatg tatgcgcctt ttttactgat gtcatcggca aaccaagtgg catacagccc
     5161 tgcgctatag cctctgacac tccctttcga acgataatcc gacacgctgg aatgagttaa
     5221 gttgtagtcg cgggcataac cagccatcac tcccaaacgc caactgtcac tatcggtaaa
     5281 accaccggtt aacagatcgc cccctaactg cgagacgtag cgatgcgagg tcgttctcaa
     5341 ctgtccgttg ctgtcacgcc aggcattatg cccgccaatt tgacgtagcc aaagtcggct
     5401 tgaacgctcc tgttcagtta caggatcgat gtagcgcgtt tcacccgcac ggtcgttcag
     5461 acgcataaca aaaagagtgt tagcggctgc caggttggcg acgtaacttc cagcttcggg
     5521 gcgcaggacg ggggtaggcg caacagtagg cgctgaactt cctccggagt ttgtttcacc
     5581 gccgctcgtt gcctggaagt tagtcagata ccagttttta ttggtcgctt cggtattacc
     5641 tcgggttaat gaatattcgt aggcaccggc gaaaatcctc gaatccttaa taaattcccc
     5701 atttgattcc ccctcaacgc tgataatctc tatcccattt aacgtgtaag ctccgctgcc
     5761 tcctgcattg gtaactttaa ctcgggttgt tccagaggta tttccattaa taacgacttt
     5821 atccgtagct gaattatcat catccaattc agttctgaga tgcaatacgc cattattacc
     5881 aacgtaattt tcagttattg ttaatcttgt tggttcgaca tcccgtccat ctgcccggga
     5941 aatatagacc gtactattat caaccgtgag gttgctcacc gttgagtcag ctttaatatc
     6001 ccaggtacta ttatctgaaa gcgacagata ggtatgagtg ttatcatctg ttgaaatatt
     6061 agctgaaccc gttatttttg aattagttgc atcgacggtc aaatgacctt taatattatt
     6121 gataaaaaca tctgcagccg atgatacctc actatccgta atggtcacgt tactcgtctc
     6181 atctttatgg tagaggattt catttgctga ccgtagtgag ctaccagata aaatatctac
     6241 cgttgcttcc tgaccattta tggaagactc cagcacaatt ccagacccct gatgcgcgac
     6301 tgcagatgta tttttcagcg tcaccgtgcc gccattataa attttagcaa tcgcggcgtc
     6361 aggacccgtt gtataaattt ctccctggtt aaatgaaact gtcccctggc gggcagcgat
     6421 ggcatgagca ttgtccccat gcgtggtgat atcaacatcg gttaatgtaa cgctggcatt
     6481 ggtataatca agcgtgtcac cagccttgac gacagtgcca ttagcgtacg cggcataact
     6541 ttctgttccg tgggttgtga tctccccttt tgccatatcc agttttgcta tggcattatt
     6601 ttcattactt cctgtatcgg cagtactttc ttcatcaaga gaagatacac acgcacacgc
     6661 gcttccggga gatgcacacg caagaacgcc gactgcgtga ttacccgcag taacaataaa
     6721 tgtatcatct ccgtcagaaa aagagggggt aaccgaaccg tcagagttga ctcttattat
     6781 ggtgtcagta ttcaggtttc cactcgtttg acttagcaaa ccaattgagt aatcccccgt
     6841 tgtgacaatc gtgtttttac cctgcaaaat aacagcaccg ccaaattcac ttacgattgc
     6901 gctagctgtt aatgctgggc ttagaggact attgtcagaa ggatataaac cgcccgttgt
     6961 tacaatgacg ccattgttaa tggttaactt actaccctct cctgcaacgg atacccgtaa
     7021 cttccattac ccgttgttat aactagcgca ttatcaatat cttttacttt tccatctttt
     7081 attgccacag catgtttata ttcatcgtgc tgaataaact gttcagcata tgcattaccc
     7141 accgccagag caataagtcc caaactaaac ccggggagtt ttttcagttt catagattaa
     7201 ctatccattc attggattaa caaacattcc tggtcaaata acctatgagc tcagattacc
     7261 aaaacggagg gtaaagacaa aaacaaaagc acaaaaaatt taaatagata aacagcgaca
     7321 atatataaat gataaataag tcggcattat catttttcac cttattttca tgacattgat
     7381 cactttgggg tgactatttt gtttaacaaa taactcttcg acatcaaaat tattaatagt
     7441 tgccatggta attatttcgg tgatatataa aaacaactgt gataacacca aggttgacaa
     7501 ctatttgcgc aataattcct ttccttcaat gatccaggcg ttcactgcca gcaacccatt
     7561 cagccgcata ctgacagtga gaaactgaaa gattattgct gatccctgtg gctaacgagt
     7621 ctttctctta aatcgattaa ccagattgat cgccagccaa acaagggcat taagcaaatt
     7681 tatcgtcaca agcacttctc ccagcccggt actgggtttt gcaataagga tgaccaggag
     7741 ggtgatcagc ataaatgcaa tggaaaagat gatatagaga gtgaaataat ccagattatt
     7801 actattattc atgctactaa tggcaatcct gtcaaaactt tcaaacaatt atagcaaaca
     7861 aaataatgaa caatagtaac aagaggcaac gagctaatta gctgtctata attacaccgc
     7921 actatggcct tactgatcaa ttttcactca accacaacct tcaccttcgg tttagaaagg
     7981 ataattttga ccaggtcgat gtatgtaaca ggtttataga aataataacc ctgctgaaat
     8041 gtgatattat tttggttcag atagccaagt tgttctttcg tttcgacacc ttcagcgacg
     8101 atactgatcg aaagtttacg cgccagatcc aatacacaat ccagaattcg ggttgattct
     8161 gggtctgcgt ttacgcggcc aacgaaactg tgatcaattt tgatataatc aatatgcagg
     8221 tcatgaagat aagaaagccc tgagtagcca gtaccgaaat catccagcgc gatgacaaaa
     8281 ccattttcat gcagaatgtt caaccgctga accagacttt catcaacatt caatggctca
     8341 cgctcggtga cttccagaac aaggtttaaa tcgcggcggg taaaactgtc acggaaattt
     8401 aaacactcgt cgacaaacgt cggcgaaata atatgcgaag cgctaaaatt aattccaata
     8461 tgaaaacctt ccggcagttt actcgcgata gcgttcatct gtctggcaac ctgattcatc
     8521 aggctttgtg taagcggaac gattaatccg gatttttcag caagtggaat aaatgccgcg
     8581 ggtgaaatat atccaccgtg aggttgtttc cagcgggcta acacctcaac tccccgcaat
     8641 gtcccttccc gaccatttac cacaggttgg taaaaaggga cgatctcccc attatttatt
     8701 gcccgtcgca ggatctcttc aggcgttgcg cttttattga aatatctatc aagcaggaag
     8761 gcagcggcgc tggcgattaa cagaataaat attaaaatcc caaagccttt atcgataagt
     8821 ctgtagaagc taaagagtgg cggcgggtta aagataatag tgaaatgata ttttgtggag
     8881 tgaaccttct cggtataggg ataatgtcca ttaagcgtca tcacatcagc cgttggtcca
     8941 ataatgtcat ccgccacgcg taatacatac cttacccctt tcaagggtac attaagcgcc
     9001 ccgcgaatat gttgatcact tatcgtaacc aaaatgcgcg tatctgcaaa ttggctctga
     9061 tagagcagga taggtaatct attaacggtg tcgattgccg gagccaacag taaattactg
     9121 tccgggacaa caggaatacg agacagcagg acccgattcc caggcaggga tgtacaccag
     9181 actattccct gtttgagaat gatgattgtg cgcaggtgag gtttcagagc tgcttcagtg
     9241 ccaagctgat attgctcctc gaggtcgcat tccttcccgg caatgttaac cgctgtttga
     9301 gtcgcctgtg acgcttcttc aagtatatga ttgatattgt tggcggcata tcttgctccc
     9361 gccaggtact ctgcttttgc ggaataccag agctggatgt tcaaaatagc gacaccggta
     9421 ataaacaagc atatcgcaac gaggatgggt atgagtgtat tgcgcatttg cttgtcccgg
     9481 ccaaagggtt taataca
//
LOCUS       DAJTRD010000129         9477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTRD010000129 DAJTRD010000000
VERSION     DAJTRD010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.129"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            73..204
                     /locus_tag="OO315_003599"
     CDS             73..204
                     /locus_tag="OO315_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5892366.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            464..1459
                     /gene="pgl"
                     /locus_tag="OO315_003600"
     CDS             464..1459
                     /gene="pgl"
                     /locus_tag="OO315_003600"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5892367.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1500..2453)
                     /locus_tag="OO315_003601"
                     /pseudo
     CDS             complement(1500..2453)
                     /locus_tag="OO315_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2637..3689
                     /locus_tag="OO315_003602"
     CDS             2637..3689
                     /locus_tag="OO315_003602"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5892368.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3765..5198
                     /locus_tag="OO315_003603"
     CDS             3765..5198
                     /locus_tag="OO315_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5892369.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5381..7561
                     /locus_tag="OO315_003604"
     CDS             5381..7561
                     /locus_tag="OO315_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5892370.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7702..8985)
                     /locus_tag="OO315_003605"
     CDS             complement(7702..8985)
                     /locus_tag="OO315_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5892371.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9120..>9477)
                     /locus_tag="OO315_003606"
     CDS             complement(9120..>9477)
                     /locus_tag="OO315_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5892372.1"
                     /translation="KIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSS
                     GTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMAS
                     QYRDDRGREWDKIEIK"
BASE COUNT         2315 a         2219 c         2424 g         2519 t
ORIGIN      
        1 cccatggaag gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca
       61 gggggattga ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat
      121 ctacaacgta atgcgtttat ttgtgccgga agtgagttga ttgttgcgcg aatgggaagt
      181 tggccagaag ttgttatggc atagtgttta cggtcgttga tacaggtaaa caaaagcggg
      241 gagagtcata ctccctgaat agtcgcgaaa atatctgtgg cattgtcaaa ttcgtgtcgg
      301 tgactaatat tttagtaata tgccattcga aatggagtgc taacttattg atttaattga
      361 tgtgcgatat tgtattttat cgtgttttgt tgtctgtttt ttatccagta agtgttgtta
      421 tatgtatgaa agataaggga tttatcattt ttgaggtgtg ttcatgaagc aaacagttta
      481 tatcgccagc cctgagagcc agcaaattca cgtctggaac ctgaatcatg aaggcgcact
      541 gacgctgaca caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga
      601 caaacgttat ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt atcgtattgc
      661 cccggacgat ggcgcactga cctttgccgc agagtctgtg ctgccgggta gtccgacgca
      721 tatttccacc gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt
      781 gagcgtaacg cgtctggaag atggcctgcc agtgggcgtc gtcgatgtag tcgaggggct
      841 ggacggttgc cattccgcca atatctcacc ggacaaccgt acgctgtggg ttccggcatt
      901 aaagcaggat cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg tggcgcagga
      961 ccctgcggaa gtgactaccg ttgaaggggc cggcccgcgt catatggtat tccatccaaa
     1021 cgaacaatat gcgtattgcg tcaatgagtt aaacagctca gtggatgtct gggaactgaa
     1081 agatccgcac ggcaatatcg aatgtgtcca gacgctggat atgatgccgg aaaacttctc
     1141 cgacacccgt tgggcggctg atattcatat caccccggat ggtcgccatt tatacgcctg
     1201 cgaccgtacc gccagtctga ttaccgtttt cagcgtttcg gaagatggca gcgtgttgag
     1261 taaagaaggc ttccagccaa cggaaaccca gccgcgcggc ttcaatgttg attacagcgg
     1321 caagtatctg attgccgctg ggcaaaaatc tcaccacatc tcggtatacg aaattgttgg
     1381 cgagcagggg ctactgcatg aaaaaggccg ctatgcggtc gggcagggac caatgtgggt
     1441 ggtggttaac gcacactaac cgctgattta accggcgcag tctctcctgc gccggtgtat
     1501 taacttatct cctgtaacgc atgtctctgg cgttcgacga tattggtcca caaattgtct
     1561 ttatcgtcag tccacaaatt aatcagcaag gaaaaaaagc gttcagctgc cggagaaagc
     1621 acggcatctt tgcggcgaat aatccccaat gtccgacgta taacgggttc caccagtggg
     1681 ataccaataa tcgaagaata gggcgcatgg ggcattgcca gaccaggaag cgccgaaatc
     1741 cccagtcctg cctccactag tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag
     1801 tccagcttcc agggcttgtc ggctagctgt tgttctatca acaatcggtt gccgctggag
     1861 gaacgtacgc caatcatttt gtaacccact aactcctgcc attctacaag ctactttttg
     1921 gccaatggat ggtcacgccg acaggccagc acgaatggct cgttgaccag tggggtaaaa
     1981 tcaatggatg aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc
     2041 accgattcca tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac
     2101 agctcattaa atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac
     2161 gctaatgtca ccataccacg atgataagca ttcatatcac gaatattaaa aagcgtttca
     2221 tcgaatttct ttattaattc ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac
     2281 gtaaccttgc gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt
     2341 attcttcgcg ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt gttaaatgaa
     2401 ctggactctg ccagtatgac aaatgccttc atacttgata attcatgctt cattattact
     2461 ccggaaaatg gaagcgacga ttttgggggg ctggtcgtta aaaattttaa ctgcatttag
     2521 ccaacttaaa ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt
     2581 tgtccttaat gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga
     2641 aaaaaatacc ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc ctgttagcgg
     2701 aacatttacc cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt atgggttccg
     2761 gtaacgatct ggaaattgac ggtattggcg gcggtaatcc actgaccagt aaagtcgcca
     2821 ttattagccg ttccagcgat ccgcgtgctg atgtcgatta tctgtttgct caagttatcg
     2881 tccatgagca acgtgtcgat accacgccta actgcggcaa tatgctgtct ggcgtagggg
     2941 cattcgccat tgaaaatggt ttgattgcag cgacttcgcc agttacccgt gtacgtatcc
     3001 gcaacgtcaa tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg
     3061 agtacgaggg tagcgccaga attgacggcg taccgggtac tgccgcaccg gttgcgctca
     3121 ctttcctgaa tgccgctgga acgaaaactg gaaaagtttt cccgactgat aatcagattg
     3181 attattttga cgatgtcccg gtgacctgta tcgatatggc gatgccagtc gtcattattc
     3241 cggctgaata tctgggaaaa acaggttatg aattaccggc ggaactggat gccgacaaag
     3301 cattattagc ccgcattgaa tctatccggc tacaagcggg taaagcaatg ggcttaggtg
     3361 atgtcagtaa tatggttatc cctaaacctg tgcttatttc tccagcgcag aaaggcgggg
     3421 caattaatgt gcgttatttt atgccgcatt cttgccatcg cgcgctggcg ataaccggtg
     3481 ctattgctat ttccagtagt tgtgcattgg aaggcaccgt cacccgacaa atcgtccctt
     3541 ctgtaggata tggcaatatc aatattgaac accccagtgg tgcgctcgac gttcatttaa
     3601 gtaatgaagg tcaggatgcc acgacgttac gcgcatctgt tattcggaca accagaaaaa
     3661 tattttccgg tgaagtttat cttccctgaa aaaattcgtt gtcaggataa ggacaatcaa
     3721 taaaggactt ctgtatgagt catacagaaa gaacaggatt ttaaatgaat aagaaatcgt
     3781 tatggaagct aattctgata ttagcgatcc catgtattat tggttttatg ccagctccgg
     3841 caggattaag cgaactggcg tgggtgcttt ttggtattta cctggcggcc attgtgggtc
     3901 tggttatcaa gcctttcccg gaacctgtcg tactgttaat tgccgttgct gcctcaatgg
     3961 tggtggtcgg taacttatcc gacggtgcgt ttaagaccac cgccgtatta agcggttact
     4021 cttcaggtac tacctggctg gtgttctcgg cgtttacctt aagcgccgca tttgtgacca
     4081 ccggtttagg taaacttatt gcctatctgc tgattggtaa aatcggtaac accatgctgg
     4141 gtctgggtta cgttacggta ttcctcgatc tggtactggc tccggcaaca ccgtctaaca
     4201 ccgcgcgtgc gggcggcatt gtgttaccga tcatcaacag cgtggcggtg gctttggggt
     4261 ccgaaccgga aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc atttacatgg
     4321 tcaccaaaac caccagctat atgttcttta ccgcaatggc ggggaacatt ctggcgctga
     4381 aaatgatcaa cgacattctg cacctgcaaa ttagctgggg tggatgggcg ctggccgccg
     4441 gattgccggg catcattatg ctgctggtca ccccgctggt aatttacacc atgtatccac
     4501 cagaaattaa aaaggtggat aacaaaacca tcgctaaagc gggccttgcc gaactgggac
     4561 cgatgaaaat ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgcta ctgggctgga
     4621 ttttcagtaa gtctctgggg gttgatgaat ccactgtggc aatcgttgtt atggcgacca
     4681 tgctgctgct gggtatcgtt acctgggaag acgtggttaa aaataaaggc ggctggaata
     4741 ccttaatctg gtacggcggt attatcggct taagctcctt attatcgaaa gttaaatttt
     4801 tcgaatggtt agctgaagtc tttaaaaata acctggcatt tgatggtcac ggtaacgttg
     4861 ctttcttcgt tattattttc ctcagcatca tcgtgcgtta tttcttcgct tccggtagtg
     4921 cctatatcgt tgctatgtta cctgtatttg ccatgctggc gaacgtctcc ggcgcaccgt
     4981 taatgttaac cgcgctggca ctgttgttct ccaactccta tggcggcatg gttactcact
     5041 atggcggcgc ggcaggtccg gtcatctttg gcgtgggtta caacgatatt aaatcctggt
     5101 ggttggtcgg tgcggtactg acgatattaa ccttcttggt gcatatcacc ctcggcgtgt
     5161 ggtggtggaa tatgctgatc ggctggaaca tgctgtaaat atactcgtca tacttcaagt
     5221 tgcatgtgct gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga
     5281 ttcactcact tgtcgccttc ctgcaactcg aattatttag agtatatcca tttattatct
     5341 ttctgcgcac ttcacggtgc gcagatatct ggagcatttg atgatcaagt tatctgaaaa
     5401 aggcgtgttt ctcgccagta ataacgaaat aattgccgaa gaacatttca ccggcgaaat
     5461 taaaaaagaa gaagcaaaaa aaggcactat tgcctggtct attctctctt ctcataatac
     5521 gtccggaaat atggataaac ttaaaattaa gtttgattca ttagcctctc acgatattac
     5581 ctttgttggt attgtacaga ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta
     5641 tgtgctgacc aactgccata actcactctg cgccgtcggc ggcaccatta acggtgatga
     5701 ccatgttttt ggtttatcgg cagctcagcg ttatggcggt atttttgtgc ctccgcatat
     5761 tgcggtcatc catcaatata tgcgtgagat gatggctggc gggggcaaaa tgatcctcgg
     5821 gtcagacagc cacacccgtt acggtgcatt agggacaatg gcagtcggtg agggtggcgg
     5881 tgagttggta aaacagctgc ttaatgacac ctgggatatc gactatccgg gcgtggttgc
     5941 ggtgcatctg accggaaaac ctgcgccgta tgtggggcca caggatgtgg cgctggctat
     6001 cattggcgcg gtgttcaaaa acggttacgt caaaaacaaa gtcatggagt tcgttggacc
     6061 gggcgttagc gcgctctcta ccgatttccg taacagcgtt gacgtgatga ccacggaaac
     6121 cacctgttta agctccgtct ggcaaactga tgaagaagtg cataactggc tggcgctgca
     6181 cggtcgcggt caggattact gccagcttaa ccctcaaccg atggcgtact acgatggctg
     6241 catcagcgtt gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa
     6301 cgtgtatgaa atcgacacac tgaaccagaa cctgaccgac attctgcgtg agattgaaat
     6361 tgagtccgaa cgcgtggcgc acggtaaagc caaactctcg ctgctggata aagtggaaaa
     6421 tggtcgcctg aaagtgcagc aggggattat cgcgggctgt tctggcggta actacgaaaa
     6481 cgtcatcgcg gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct
     6541 ggcggtttac ccgtcatcac agccggtgtt tatggatctc gccaaaaaag gtgtggtagc
     6601 agatttgatt ggcgcaggcg caatcatcag aaccgcattc tgtggcccat gctttggcgc
     6661 gggcgatacg ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa
     6721 ccgcgaaggc tctaagccag ccaatgggca gatgtcagcg gtggcgttga tggacgctcg
     6781 ttctatcgct gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aacttgattg
     6841 ctgggacaac gtgccggagt acgccttcga tgtaacgccg tataaaaacc gtgtttatca
     6901 gggctttgtg aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca ttaaagactg
     6961 gccggaattg ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga
     7021 cgaagtcacc accaccgacg aactgattcc ttccggtgaa acctcttctt atcgttcaaa
     7081 tccgattggt ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca
     7141 gattgctggt caggagcata ttgatccgct gcaaactgaa attggcagca tggtctatgc
     7201 ggtgaaacca ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg
     7261 cggtctggcg aatattgccg aggagtacgc gactaaacgc taccgttcta acgtcatcaa
     7321 ctgggggatg ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat
     7381 ttacatccct ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt
     7441 gatccatgaa gatgcgccgg taacggaaat tacgctttac atggaaagcc tgacggctga
     7501 agagcgcgag attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta
     7561 aaaagcgcca tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgctggcgc
     7621 atcttatccg acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat
     7681 ccggcaaata gttaatagct cttacttctt ctcctctgca accactttgc tgcccacgcc
     7741 gcggttattg tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc
     7801 gtcgttatca tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc
     7861 ggcccacggt ttagccgtgt taaaaccttc gttgatggcg ctatcacgga tcaccacctg
     7921 accgttggta ttagcatcaa catccagcga gcggcccagt tgcgccacgc catcaccgga
     7981 agcattgaaa cggctgttta cggcgaggaa accgtaatag atgttagaca gcgtagccgg
     8041 tgcaaacaca tatgcttctt gctgggtacg tgagttcacc acgcggaatt cggtgttatc
     8101 gaacaccact gcgccgcgac cagaaacgat atccacatcc ccttcaatat agctgttggt
     8161 caccagcgta cgcggctgac ggttggtttc cagacggttc tgcacaccgc tgttggtgac
     8221 aaagaaggtg ttctgacgac cgagaatgtt aacgttgtta atctgtactt ggtcaccgtc
     8281 agtacgcagt gctaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc
     8341 gatggtcaga ttttgcagtt gcagtccatt gttttgtgac cagaagaccg cagaacacag
     8401 aacaccgata ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt
     8461 acctggcata tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc
     8521 cccatcaagg gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt
     8581 gattccacct ggagcagcag ggacatacac cgttccctga tactcaccag gcatcacggc
     8641 aatatactgg cgcttgttgg tgcgcttgat aattgccgca tctaccgccg cctgaatcgt
     8701 ggtatgcgtt acaccttgag tacccgccgg gccgacaaca aagtcaggtt gcgcaggcag
     8761 ggttatcggg gacggattcc acgctgcagc acctggtgtc agggatgcaa aatagtgttg
     8821 cgcatcgaaa ttcagcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc
     8881 agtttgatca gaaggacgtt gatcgggtgg tgttgagcta caggcggtca gcgtcacgcc
     8941 aaaagccaat gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct
     9001 atgaaataga aaaatgaatc cgttgaagcc tgctttttta tactaagttg gcattataaa
     9061 aaagcattgc ttatcaattt gttgcaacga acaggtcatt atcagtcaaa ataaaatcat
     9121 tatttgattt caattttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg
     9181 gtgtccgact tgtgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag
     9241 agtcttgcag acaaactgcg caactcgtga aaggtaggtg gatccccttc gaaggaaaga
     9301 cctgatgctt ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttca
     9361 cgacgagtag atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt
     9421 tccttcattg atatcccgag agcatcaaca tgcaatgttg tagggatggc aattttt
//
LOCUS       DAJTRD010000130         9402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTRD010000130 DAJTRD010000000
VERSION     DAJTRD010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.130"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            70..222
                     /gene="hokE"
                     /locus_tag="OO315_003607"
     CDS             70..222
                     /gene="hokE"
                     /locus_tag="OO315_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5892373.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            573..725
                     /gene="hokE"
                     /locus_tag="OO315_003608"
     CDS             573..725
                     /gene="hokE"
                     /locus_tag="OO315_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5892374.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(847..1467)
                     /gene="entD"
                     /locus_tag="OO315_003609"
     CDS             complement(847..1467)
                     /gene="entD"
                     /locus_tag="OO315_003609"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5892375.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="OO315_003610"
     CDS             complement(1642..3882)
                     /gene="fepA"
                     /locus_tag="OO315_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5892376.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4125..5327
                     /gene="fes"
                     /locus_tag="OO315_003611"
     CDS             4125..5327
                     /gene="fes"
                     /locus_tag="OO315_003611"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5892377.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5330..5548
                     /gene="ybdZ"
                     /locus_tag="OO315_003612"
     CDS             5330..5548
                     /gene="ybdZ"
                     /locus_tag="OO315_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5892378.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5545..9390
                     /gene="entF"
                     /locus_tag="OO315_003613"
     CDS             5545..9390
                     /gene="entF"
                     /locus_tag="OO315_003613"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5892379.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGIAARLRARGEQV
                     AFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGN
                     YADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDI
                     ISPGAFVKIGPIIRATLNR"
BASE COUNT         2044 a         2474 c         2598 g         2286 t
ORIGIN      
        1 cctggggctg ccactccaaa ccagacaatt tggatggtag tcccttcttc gcatggaggt
       61 aatataaaca tgctgacgaa atatgccctt gtggcagtca tagtgctgtg tttgacggta
      121 ctgggattta cgcttctggc cggagactcg ctgtgtgagt ttacggtgaa ggaacgtaat
      181 attgagttca gggctgttct cgcttacgaa ccgaagaagt agccgttgtg cggggagtaa
      241 tccccgcata tccggttgtc aggtcaggat ggtaaggcac ctgctttaca ctttcgcccg
      301 cggtcagtga tggctgcggg cgatttcgtt ttaagagcaa tgatgagttt tgccgacaga
      361 agtccgaaac aggactcact gtataaataa acagctattt tgctaaggaa gggtaagata
      421 acggcgggtg cctgaagctt tccggtttca ggttaactct gaggtttgga aagatgaagc
      481 cccaagagat atttctatca atcctggggc tgccactccg aacctcaaca actcggatgg
      541 tagtcccttc ttcgcatgga ggcaatataa acatgctgac gaaatatgcc cttgtggcag
      601 tcatagtgct gtgtttgacg gtgctgggat ttacgcttct ggtcggagac tcgctgtgtg
      661 agttcaccgt aaaggaacgt aatattgagt ttaaggctgt tcttgcttac gaaccgaaga
      721 agtagccgtt gtgcggggag taatccccgc atatccggtt gtcaggtcag agtggtaagg
      781 cacctgcttt acactttcgc ccgcagtcag tgatggctgc gggcgaatcg taccagatgt
      841 tgtcaattaa tcgtgttggc acagagttat gactattttt tcttttatct gccagtgcac
      901 agcaaacatc tcattctcac gatgaatgat gacctgctgt ttattccagc taattatctg
      961 atagtccaga aaacctgcat cagtttggat ctcacttgcc ttaaatgcgc tctctttggc
     1021 ggaaaatgca agtgtcagcg ccagagaaaa ggctaaaccg cagtctgcga gtcgctcgtg
     1081 ttctgctggt gtaataatgt tgtctgtcaa ttcggtcgct gtttgcgcag aaaaaatttc
     1141 ttcgatatca acgccaatcg gttgacggga aaccacggcc aatgccgtag ccccgcagtg
     1201 gctaatactg ccgtatacct ccgcaggcca caccggttgg cgtagctcgc cgattgcggg
     1261 cacacattta tagccatatt cccgcaaggc ataaacagca gcgatccgtc cggctaaatg
     1321 ctctgtttta cgtttacgtc cagcgtgttg cagttgtgcg tagtgcggca gccagagtaa
     1381 atcctgctca caaaaattcg ccggatcgaa ctcaacaaaa tgcagcgtat gtccggcaaa
     1441 ggggagggag gtatgcgtag ttttcatatc gaccatattc gagactgatg acaaacgcaa
     1501 aactgcctga tgcgctacgc ttatcaggcc tacgtgatcc tgcaatatat tgaattggca
     1561 agatctttgt aggccggata aggcgttcac gccgcatccg gcgtgaacga cgcgcacttt
     1621 gtcaacaatc tgacgttagc atcagaagtg agtattaatg ctcatatacc acgtacgtcc
     1681 cggctcgtta taggtatacg cgccggcacc ggcgatatag ttggctcctg ccagatcgcc
     1741 cgtggtctgg gcattacctg cacgccacaa acgtttgtcg aacaggttgt ccacgccgcc
     1801 ggtcagactg acattcttcg tcacatccca ggtcgcgctc agacccacaa tgctgtaagg
     1861 actgatttct ttggtttccg ggccaaccgc tggctgacct ttatagttgt acttcttcgg
     1921 ctgctgcttg ccgtaccagg tgaaggtcgt ttgcatcgac aaatcttccc gtgcctgcca
     1981 gctcagcgtt gagttcaacg tgtactccgg gatgatcgac aaacggtcgc ccgtggtttt
     2041 gttttcactc ttcagcatat aagtgatgtt attggtccac atcaccgttt cgctaactgg
     2101 tacgtttaac gatccttcca gaccttcaac cactgctttc ggcacgttat cccactgata
     2161 gagatcagtg ccgactgcgt tttgccctac agccacatag cccgcttcaa tcttattgcg
     2221 ataatcgtta cggaaccagg tgacgcccgc cagccagccg tcgcgtttga actccagacc
     2281 aatctctttg ttgatgctgg tttctacttt caggtcatcg ttaccttgca gatagcagcc
     2341 gcccgcgctg gcatagcagc cctgaccttt actgtagaga atgtagttcg ggttagtctg
     2401 gtataggctc ggcgctttat aggcgcgggc aatgcctatt ttcagcgtga agtcatcgcc
     2461 taaaccttgc gatatgttca gcgccgggct ccagttattg ccgacaatac tgtgatgatc
     2521 gaaacgcagc cccggcgtta cgatggtgct gtcagtcagc tccatgttgt tttcggcaaa
     2581 cagcgagaaa atttctgctt ttgaatacgg gctacggtcg gtggcactca cgccatcaat
     2641 agcgccaccg gtattcgttc cggtcagtgc ttgggtgttg gaacttaagt ccttcatccg
     2701 ttgctgattc cactccgtac ccagcgtcag cgtctggtta acgaggaaat caatcggcag
     2761 gttaacttcg ctgtgcagca tcacgtcatc gagatcgata tcgacgaaat cctgtgtcgc
     2821 tttttcgtta aatttccctt cggtaccgcc cgccagacct tccggaatac gcgagttacg
     2881 ggtgtgttca tactgcaccc agttgctggt ggtcacgccg ttatcccagc caccgttcca
     2941 ggtcagcgcg tagttctggc gatacaggcg gttggtttca tcgccatatt tcgagcgagt
     3001 gtaagcgtca gagttggtgt tctgggtatc gcccgcatac aggttaccct ggcggctgta
     3061 acccgcttcc agttccagcg attgcaatgg tgcgaagtcc cagcgcacca cgccgttaat
     3121 atctttattg atcacccctt cgcgcccggc tggtaacgtc gtggcatacg ttccggcacg
     3181 cgcggactga tggccctggt tgatatccca cgcgtcagcc tgagttttgt cgaggttgcc
     3241 atacaagcgg aagctgaatt cgtcgcccag cggaccggtc aggctaaagt tagtgcgttt
     3301 ggtggcacct tcctctttat gttctggcgc attgaaatat gcgtcccagg agccgtgcca
     3361 ctcgccgctg ccttttttgg taatgatgtt aaccacgccg cccgccgcgc cgttgccata
     3421 acgcgcagct gccggaccac gcagaacttc aatacgttca atcatttcag gtggcaccca
     3481 ggaggtatca ccacgggtat cgcgctcgcc acgccagccc tgacgcaccg agttacggct
     3541 gcttaccggc ttgccgtcaa tcaaaatcag cgtgttttcc ggacccatac cgcgaatatc
     3601 aatctgtcgg ttattcccac gctgaccact ggtggagtta ccggtcaggt taacgcctgg
     3661 catggtacgg atgatctccg acacatcgcg ggcaaccggg tttttgcgga tttcatctgc
     3721 ggtgatggtc gaaacgccag gagcctgcaa gttctgctcg gcggcggtaa cgacaatggt
     3781 atcgtcatgt gaaacaggtg tatcggtcgg ctcttgtgcc tgcgctaccc cataaatccc
     3841 cagattgacc aacaaggcca gggaatgaat cttcttgttc attgttttat tcctgcattt
     3901 ttgccacgaa ttgcaactgt cgggcatggt cgtcatcaac acgacgcatc ccgttaccgc
     3961 gaaaaccttt gatcctgaaa gacacgcagt gcagttggtt aattaatgtc cgcgcttccc
     4021 acggcgcgcc attacgctat tgcaaatgca aatagttatt aataacatta tcaatatatt
     4081 tctgcaatca atgaaaaatt gcacagtaaa catggggtta tggtgtgacg gcgttaaaag
     4141 taggaagtga gagctggtgg cagtcgaaac atggcccgga atggcagcgt ctgaatgacg
     4201 aaatgtttga ggtcactttc tggtggcgtg atccccaagg ttctgaagaa tactcgacga
     4261 taaagcgcgt atgggtctac atcactggtg tgaccgatca tcatcagaac agccagcccc
     4321 agtcgatgca gcgaattgca ggtactgatg tctggcagtg gacgacacaa ctcaatgcca
     4381 actggcgcgg cagctactgc tttattccca ccgaacgcga tgacattttt tctgcaccat
     4441 cccccgatcg cctcgaatta cgtgaaggct ggcgaaaact attaccccag gcaatagccg
     4501 atccgctgaa tccacaaagc tggaaaggcg ggctagggca cgctgtttct gcactcgaaa
     4561 tgccgcaagc gcctctgcaa ccgggatggg attgtccgca agcgccagaa ataccagcca
     4621 aagaaattat ctggaaaagt gaacggttga aaaattcacg tcgtgtatgg atttttacca
     4681 ccggcgatgt aacagcagaa gaacgcccgc tggcagtttt gctcgatggc gaattttggg
     4741 cgcaaagtat gcccgtctgg cctgtgctga cttcgctgac ccatcgtcag caacttcctc
     4801 ccgccgtgta tgtgttgatc gacgctatcg acaccacgca ccgcgcccac gaactgccgt
     4861 gtaatgcgga tttctggctg gcagtacagc aagagttatt acccctggtg aaagttattg
     4921 ccccttttag cgatcgcgcc gatcgcaccg tggtcgccgg gcagagtttt ggtgggctgt
     4981 ccgcgctgta tgccggactg cactggcctg aacgctttgg ctgtgtatta agccagtcag
     5041 gatcgtactg gtggccgcat cggggcgggc agcaagaggg cgtgttactt gaaaagctaa
     5101 aagctggtga agttagcgcc gaaggtctgc gcattgtgct ggaagcgggt attcgcgagc
     5161 cgatgatcat gcgggccaat caggcgctgt atgcgcaatt acaccctata aaagaatcca
     5221 ttttctggcg tcaggttgac ggcggacatg atgcgctttg ttggcgcggt ggcttgatgc
     5281 aggggttaat cgacctctgg caaccacttt tccatgacag gagttgaata tggcattcag
     5341 taatcccttc gatgatccac agggagcgtt ttacattttg cgcaatgcgc aggggcaatt
     5401 cagtctgtgg ccgcaacaat gcgccttacc ggcaggctgg gacattgtgt gtcagccgca
     5461 gtcacaagag tcctgccagc agtggcttga agcccactgg cgtactctga caccggcgaa
     5521 tttcacccag ttgcaggagg cacaatgagc cagcatttac ctttggtcgc cgcacagccc
     5581 ggcatctgga tggcagaaaa actgtcggat ttaccctccg cctggagcgt ggcgcattac
     5641 gttgagttaa ccggagaggt tgatgcgcca ttactggccc gcgcggtggt tgccggatta
     5701 gcgcaagcag atactttgcg gatgcgtttt acggaagata acggcgaagt ctggcaatgg
     5761 gtcgatgatg cgctgatatt cgaactgcca gaaattatcg acctgcgaac caatattgat
     5821 ccgcacggta ctgcgcaggc attaatgctg gcggatttgc aacaagattt gcgcgtcgat
     5881 agcggtaaac cactggtctt tcatcaactg atacaggtgg cagataaccg ctggtactgg
     5941 tatcagcgtt atcaccattt actggtcgat ggcttcagtt ttccggccat tacccgccag
     6001 atcgccaaca tttactgcgc attgctgcgt ggcgaaccaa cgcctgcttc gccgtttacg
     6061 cctttcgctg atgtagtgga agagtaccag caataccgcg aaagcgaagc ctggcagcgt
     6121 gatgcggcat tctgggtgga acagcgtcgc caactgccac cgcccgcgtc actttctccg
     6181 gcacctttag cggggcgcag cgcctcggca gatattttgc gtctgaaaat ggaatttacc
     6241 gacggggaat tccgccagct ggctacgcaa ctttcaggtg tgcagcgtac cgatttagcc
     6301 cttgcgctga cggctttgtg gctggggcga ttgtgcaatc gcatggacta cgccgccgga
     6361 tttatcttta tgcgtcgact gggctcggcg gcgctgacgg ctaccggacc cgtgctcaac
     6421 gttttgccgc tgggtattca tattgcggcg caagaaacgc tgccggaact ggcaacccga
     6481 ctggcaaccc gactggcagc acaactgaaa aaaatgcgtc gtcatcaacg ttacgatgcc
     6541 gaacaaattg tccgtgacag cgggcgagcg gcaggtgatg aaccgctgtt tggtccggta
     6601 ctcaatatta aggtatttga ttaccaactg gatattcctg gtgttcaggc gcaaacccat
     6661 accctggcaa ccggtccggt taatgacctt gaactggccc tgttcccgga tgaacacggt
     6721 gatttgagta ttgagatcct cgccaataaa cagcgttacg atgagccaac gttaatccag
     6781 catgctgaac gcctgaaaat gctgattgcc cagttcgccg cggatccggc gctgttgtgc
     6841 ggcgatgtcg atattatgct gccaggtgag tatgcgcagc tggcgcagat caacgccact
     6901 caggttgaga ttccagaaac cacgctgagc gcgctggtgg cagaacaagc ggcaaaaaca
     6961 ccggatgctc cggcgctggc agatgcgcgt tacctgttca gctatcggga aatgcacgag
     7021 caggtggtgg cgctggcgaa tctgctgcgt gagcgcggcg ttaaaccagg ggacagcgtg
     7081 gcggtggcac taccgcgctc ggtctttttg accctggcac tccatgcaat agttgaagct
     7141 ggcgcggcct ggctaccgct ggataccggc tatccggacg atcgcctgaa aatgatgctg
     7201 gaagatgcgc gtccgtcgct gttaattacc accgacgatc aactgccgcg ctttagcgat
     7261 gttcccaatt taaccaacct ttgctataac gccccgctta ccccccaggg cagtgcgccg
     7321 ctgcaacttt cacaaccgca ccacacggct tatatcatct ttacctctgg ctccaccggc
     7381 aggccgaaag gggtaatggt cgggcagacg gctatcgtca accgcctgtt gtggattcaa
     7441 aatcattatc cgctcacagg cgaagatgtc gttgcccaaa aaacgccgtg cagttttgat
     7501 gtctcggtgt gggagttttt ctggccgttt attgccgggg ctaaactggt gatggctgaa
     7561 ccggaagcgc accgcgaccc gctcgctatg cagcaattct ttgccgaata tggcgtaacg
     7621 accacgcact ttgtgccgtc gatgctggca gcatttgttg cctcgctgac tccgcaaacc
     7681 gctcgccaga gttgcgtgac gttgaaacag gttttctgta gtggtgaggc cttaccggct
     7741 gatttatgcc gcgaatggca acagttaacc ggcgcgccgt tgcataacct gtatggcccg
     7801 acggaagcgg cggtagatgt cagctggtat ccggcttttg gcgaggaact ggcacaggtg
     7861 cgcggcagca gtgtgccgat tggttatccg gtatggaata cgggtctgcg tattcttgat
     7921 gcgatgatgc atccggtgcc gccgggtgtg gcgggtgatc tctatctcac cggtattcaa
     7981 ctggcgcagg ggtatcttgg acgacccgat ctgaccgcca gccgctttat tgccgatcct
     8041 tttgctcctg gtgaacggat gtatcgtacc ggagacgttg cccgctggct ggataacggc
     8101 gcggtggagt acctcgggcg cagtgacgat cagctaaaaa ttcgcgggca gcgtatcgaa
     8161 ctgggcgaaa tcgatcgcgt gatgcaggcg ctgccggatg tcaaacaagc cgttacccac
     8221 gcctgtgtga ttaaccaggc ggcagccact ggtggtgatg cgcgtcagtt ggtgggctat
     8281 ctggtatcgc aatcgggcct gccgttggat accagcgcat tgcaggcgca gcttcgcgaa
     8341 acattgccgc cgcatatggt gccagtcgtt ctgctgcaac tgccgcagtt gccacttagc
     8401 gccaacggca agctggatcg caaagcctta ccgttgcctg aactgaaggc acaagcgcca
     8461 gggcgtgcgc cgaaagcggg cagtgaaacg attatcgccg cggcattcgc gtcgttgctg
     8521 ggttgtgacg tgcaggatgc cgatgctgat ttctttgcgc tgggcggtca ttcgctactg
     8581 gcaatgaaac tggcagcgca gttaagtcga cagtttgctc gtcaggtgac gccggggcag
     8641 gtgatggtcg cgtcaaccgt cgccaaactg gcaacgatta ttgatggtga agaggacagc
     8701 tcccggcgca tgggattcga aaccattctg ccgttgcgtg aaggtaatgg cccgacgctg
     8761 ttttgtttcc atccggcgtc cggttttgcc tggcagttta gcgtgctctc gcgttatctc
     8821 gatccacaat ggtcgattat tggcattcag tcgccgcgcc ctcatggacc catgcagaca
     8881 gcgacaaacc tggatgaagt ctgcgaagcg catctggcaa cgctacttga gcaacaaccg
     8941 cacggcattg cggcgcggct gcgtgcccgt ggcgaacagg tggcatttct tggtttgctg
     9001 gatacctggc cgccagagac gcaaaactgg caggaaaaag aggctaatgg tctggacccg
     9061 gaagtgctgg cggagattaa ccgcgaacgc gaggccttcc tggcggcaca gcagggaagt
     9121 acttcaacgg agttgtttac caccattgaa ggcaactacg ctgatgctgt gcgcctgctg
     9181 acgactgctc atagcgtacc gtttgatggt aaagcgacgc tgtttgttgc tgaacgcacg
     9241 cttcaggaag gtatgagccc cgaacgcgcc tggtcgccgt ggattgcgga gctggatatc
     9301 tatcgtcagg attgtgcgca tgtggatatt atctctccag gggcgtttgt aaaaattggg
     9361 ccgattattc gcgcaacgct aaacaggtaa attaatatta tc
//
LOCUS       DAJTRD010000131         9281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTRD010000131 DAJTRD010000000
VERSION     DAJTRD010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.131"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..276)
                     /locus_tag="OO315_003614"
     CDS             complement(<1..276)
                     /locus_tag="OO315_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HCR5892380.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWF"
     gene            complement(468..2750)
                     /gene="pflB"
                     /locus_tag="OO315_003615"
     CDS             complement(468..2750)
                     /gene="pflB"
                     /locus_tag="OO315_003615"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5892381.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(2805..3662)
                     /gene="focA"
                     /locus_tag="OO315_003616"
     CDS             complement(2805..3662)
                     /gene="focA"
                     /locus_tag="OO315_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5892382.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4068..5828)
                     /locus_tag="OO315_003617"
     CDS             complement(4068..5828)
                     /locus_tag="OO315_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5892383.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5958..6584
                     /locus_tag="OO315_003618"
                     /pseudo
     CDS             5958..6584
                     /locus_tag="OO315_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            6783..7871
                     /gene="serC"
                     /locus_tag="OO315_003619"
     CDS             6783..7871
                     /gene="serC"
                     /locus_tag="OO315_003619"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5892384.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            7942..9225
                     /gene="aroA"
                     /locus_tag="OO315_003620"
     CDS             7942..9225
                     /gene="aroA"
                     /locus_tag="OO315_003620"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5892385.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT         2359 a         2222 c         2339 g         2361 t
ORIGIN      
        1 gaaccagtca cgaacaaact cagcttgcag gattgcttca ccgccggatg cggtaacgcc
       61 gccgccggaa gcgttcataa agtggcgata ggtcaccact tccttcatca aatcttcaac
      121 ggtaacttct ttaccgccat gcgtgtccca ggtgtcgcgg ttatgacaat acaggcagcg
      181 catcaggcag ccctggaaaa aggtgataaa gcgaatacct gggccgtcta cggttccaca
      241 ggattcaaag gagtgaatgc gaccaataac tgacattgcg gtgtttctcc agatgtggcc
      301 catctgaggc cgtgttggtg cgcagctcga aggctacgtc gagtctgttt tggcagtcac
      361 cttaaagtat agatagctga caaaaaaggc tctcgtgcta aaaaaggccc cactttcgtg
      421 gagcctttat tgtacgcttt ttactgtacg atttcagtca aatctaatta catagattga
      481 gtgaaggtac gagtaataac gtcctgctgc tgttctttag tcagcgagtt gaaacgtact
      541 gcgtagccag atacacggat ggtcagctgc ggatattttt ccgggttttc catcgcgtcg
      601 agcagcattt cacggttcat cacgttaacg ttcaggtgct gaccaccttc gatggatgct
      661 tcgtggtgga agtaaccatc catcagacca gccaggttgg tcttacgaac ttcgtcgtct
      721 ttacccagtg cgttcggaac gatagagaag gtgtaggaga taccatcttt agcgtaagca
      781 aacggcagtt tagcaacgga agtcagagac gctacagcac ctttctggtc acgaccgtgc
      841 atcgggttag cacccggtcc gaacggcgcg ccagcacgac gaccgtctgg ggtgttacca
      901 gttttcttac catacacaac gttagaagtg atggtcagaa cagactgagt cgggatagcg
      961 tcacggtagg tgtgcagttt ctgaattttc ttcatgaaac gttctaccag gtcaacagcc
     1021 aggtcatcta cacgcggatc attgttacca aactgcgggt attcgccttc gatttcgaag
     1081 tcgatagcca gaccgtcttc gtcacgaatc ggtttaactt tcgcatattt gattgcagac
     1141 agggagtcag cagcaacgga cagaccagcg ataccacacg ccatggtgcg gataacgtca
     1201 cggtcgtgca gcgccatcag agaggcttcg tagctgtact tgtcgtgcat gtagtggatg
     1261 atgttcagtg cagtgatgta ctgtttagcc agccagtcca tgaagtgatc catgcgctcc
     1321 atcacttcat cgtagctcag gagatcgcct ttgatcggtt cagacttcgg accaacctgc
     1381 attttcagtt tttcgtcaac gccgccgttg attgcgtaca gcatggtttt cgccaggttt
     1441 gcacgcgcac cgaagaactg catttgttta ccaacgatca tcgggcttac gcagcaagcg
     1501 atagcgtagt catcgttgtt gaagtccgga cgcatcaggt catcgttctc atactgcaga
     1561 gaagaggtgt cgatggacac tttagcggcg aatttcttga agttcagcgg cagtttttca
     1621 gaccacagaa tggtcatgtt cggttccgga gacggcccca tagtgtacag ggtgttcagg
     1681 aaacggaagc tgtttttggt aaccagggta cgaccatcga ggcccatacc accgatagat
     1741 tcggttgccc agattgggtc gccagagaac agttcatcgt attccggagt acgcaggaag
     1801 cgaaccatac gcagtttcat gaccaggtgg tcaaccattt cctgcgcttc ttgttcggtg
     1861 atcttgccag ctttcaggtc acgttcgatg tacacatcca ggaaggtgga ggtacgaccg
     1921 aaggacattg cagcaccgtt ctgagactta acagcagcca ggtagccgaa gtaagtccac
     1981 tggatagctt cctgagcgtt ggtagccgga ccagagatgt cgtagccgta tttcgcagcc
     2041 atttctttca tctgacccag agcgcggtgc tgttcagcga tttcttcgcg cagacggata
     2101 gtctgttcca ggtttacgcc gttttccaga tcagcctgta gagaggtgaa ctgagcgtat
     2161 ttgtctttca tcaggtagtc gataccgtac agcgcaacgc gacggtagtc accgatgata
     2221 cggccacggc cataagcatc tggcagaccg gtcagaacac cggatttacg gcaacgcagg
     2281 atgtccggag tgtaaacgtc gaacacgccc tggttgtgag ttttacggta ttcagtgaag
     2341 atttttttga tcatcgggtc cagttcgcgg ttgtacgctt tgcaggaacc ttcgatcatt
     2401 ttgataccac cgaacgggat aagagcacgt ttcagcggag cttcagtctg cagaccaaca
     2461 actttttcca acgctttatt gatgtagcca gcgtcgtgag aggtgatggt ggaagcaaca
     2521 gcggtgtcaa aatcaactgg cgcgtgagtg cggttttcca gtttaacacc ttccattact
     2581 ttgtcccaca gggtggtggt cgcttcagta gcgccagcca ggaaggactc gtcaccctcg
     2641 tacggagtgt agtttttctg aatgaagtca cggacgttta cttcattctg ccagtcacct
     2701 ttggtaaaac cttcccaggc tgtggctaac ttttcattaa gctcggacat gtaacaccta
     2761 ccttcttaag tggatttttt atttactgcg tacttcgaca accattaatg gtgatcgttt
     2821 tcacgcaggt aaatgaccca gtatgtcaac ccaaccaaca aaccaccgcc gataatatta
     2881 ccgatcgtaa ccgggatcag gttatcagtg atgaagttca tcacggtcag gtgagaaaaa
     2941 ttttccggtg cagaaccgac agcggtccag aattccggag atgcgaagtc gcggattaca
     3001 atacccatcg ggatcataaa catgtttgcg atactgtgct caaaaccgct ggcaacaaac
     3061 atcgcgaccg gcagcaccat aatgaacgct ttgtccatca ggctgcggcc agaataactc
     3121 atccatactg ccagacatac catcaggttt gccaggatac caagacagac ggcctcaata
     3181 aaagtatggt gcactttgtg gtcggcggtt tgtaggacgt ttagtcccca ttgaccattt
     3241 gcggtcatat actcgccgga aagccacatt aaaagtacaa acagcagtgc gccgaccagg
     3301 ttgccaaaat agacatttag ccagttttta gccaactgac accaggtgat gcgcccactc
     3361 gccttagcaa caacaatcaa cactgtggaa gtaaagagat cggctccgca gacaacacaa
     3421 agaatcagcc ccagagagaa gcaaatgccg ccaaccagtt ttgccatgcc gaagggcatt
     3481 gtgcctgtgc cagtggttgc tgtgatatag aagacgaatg cgattgagat gaaaacaccg
     3541 gcggtaatcg ccagatagaa agtcttaagc ggatgtttcg ttgctttata gacacccgcc
     3601 tcttcggcca ctttggccat tgcagcagga agtaaaagat caaaagggtt gtcagctttc
     3661 acactaactc tctctttatt aagtcggcga cgagatacta acaaagcatt atagatgaga
     3721 aattgatata gatcatatct cgcctggctt ataggcccgt aactcgcatg gtttttatgc
     3781 aaatacggag taactatttg attatccaaa taaaaataaa ttttaaaaat taacaaatga
     3841 gttgaatttt ttccgcatcc tccgctaaaa cagttaatta aaagggagca tcaggcgaat
     3901 aaagtaacaa tatcgatcgt atttattaaa tacaaattac cgatatttaa ccttataatt
     3961 acaattattt tattaatgca aatatatgta aagcggggca ttaaaaaaac gccccgtaat
     4021 ataactcaga ctaatcatta agcctacatt gcgtaggcta tttgatttta ttttgcccag
     4081 aatgctgctt tggcgcgctg cagcttttcg taggccttca acaacgactg atgtgcagca
     4141 aacgcgtgca gatcgctatc taccggttgc aggccgtaaa acgccgcttc gccgctcatt
     4201 gccgcactgg cggcttctac ggcatctgcg ccgtacatgc gaacaaacgc attcagatat
     4261 tgcagcggct ggcgatcttc ttcctgtgcc agtaataaca acgtttgcag gcagcgataa
     4321 tagttggcgc gttccggact aaatactgat gagttaaact ccatcgtcca ttcggtccag
     4381 accagagcct gttccagatc gccaccagcc agcgccagca tggcttttaa ttcaccgata
     4441 cgcagggtgt accaaccgtt atccgacccg gtcgccagac ccaacagctc acgcacgcgg
     4501 gtaaagtcat caaaaccttc ttcatccagt tgctcgatga ggttcaggta atcttctttt
     4561 tcccactcgc tgcctggtag cgaaagaatc gtttcacgta aatggctgcc catactgtta
     4621 ttcgcgagcc acagatcttc agccggataa atatcggaca tgccaggcac gataatacgg
     4681 caagcataaa cgcccagatg ctcgtaatcg gcaatataaa cttctttatc ttctttgttg
     4741 aagatagcca tcagcgtagc aaactcttct tccgtggtgc cggagaaatt ccagtccaca
     4801 aacggataat cggcatcctg cttgaacagg tcccaggaga ttaaaccgct ggaatcgata
     4861 aagtgcgttt cgaggttggt atgttcagcg acttcttcat catcgaaggt tggcggagta
     4921 aacacatcca aatctttcag gccacgacct tgcagcagct cggtcacggt acgttccagt
     4981 gctacgccaa aatcaggatg cgcaccgaaa gaggcaaagc aggtgccgtt tgcaggatta
     5041 aacagtacca cgcaaatcac cggatactgg ccgccaaggg aaccatcata tgcgaagatc
     5101 gggaaacctt ctgtttccag tgtttcgatc gcttcaacca ccgctgggta acgcgccagc
     5161 acgtctgccg ggatttccgg caagctgatg ctttcagcaa taatgcggtt tttcacgtag
     5221 cgttcgaaaa cttcggacaa cccctgaacg cgtgcttcgt tgcgggtatt gccagcggac
     5281 ataccgttag aaacgtacag gttaccaatg atattcatcg gaatataaac ggtctgattg
     5341 tcggactggc gcgtaaacgg cagaccgcaa ataccacgat cttcgttacc ggattgtagg
     5401 tcaatcagca tactgccggt caattcattc tccggatcgt aaaatgcgcg cagacggtca
     5461 tcgagcagcc cttctggcac atcgtcattt tcgctcagtg ggaaccattt ttcgttggga
     5521 taatgcacaa atggaccgtt ggcgatggtt tcgcccagcc agaaatcagc aaaaaagtag
     5581 ttggttgaga gacgctcgaa atattcaccg agtgcagaag ccagcgccgc tttcttggtt
     5641 gcgcctttac cgttggtaaa acacagtgcg cactctttgt cgcgaatatg tacagaccag
     5701 acgttaggca cgggattcag ccaggaggcc tcttcaatct gaaagccgag gtctgaaagt
     5761 ttttgctgga agcgagcgat ggaatcttcc agagcggcat ctttgccggg gataaatgtt
     5821 tgcgtcatga aaatcacttt agtcgtacgg aaagcgcgca ataatacggg ttttatctca
     5881 aaggcgctat caccgccgcc atgccgatga actgttgact atgcttttag cggataaacc
     5941 acgttaagag cataaaaatg aaggcgttcg atctccaccg tatggcattt gataaagtgc
     6001 cttttgattt ccttggcgaa gttgcactac gtagtcttta tacctttgta ctggtttttt
     6061 gttcctcaaa atgaccggaa gacgcggtgt gcgacagatg tcgctgtttg aagtgttaat
     6121 cattctcacg ctgggttcag cggcgggaga tgtggcgttt tatgatgatg tgccgatggt
     6181 gccggtactt atcgtcttta tcactctggc gttgttatac cgcctggtaa tgtgatggcg
     6241 agctggcctg gtcgaaactc aataactcca acatgacgga atttgagttc tttatggagc
     6301 tacgattgcg tggcgtggag cagctggggc aggtacgtct ggcgattctc gaaaccaacg
     6361 ggcaaatcag tgtctatttc tttgaagatg acaaggtgaa accgggttta cttattttac
     6421 ccagtgattg tacgcagcgt tacaaagtgg tgccggagtc ggcggactat gcctgcattc
     6481 gttgtagtga aatcattcat atgaacgcgg gggaaaaaca attatgtccg cgctgtgcaa
     6541 atccagaatg gacgaaggca agtcgggcaa aacgggtgac ctgacagtaa aaacatcggc
     6601 tttttgctaa taatccgaga gattcttttg tgtgatgcaa gccacatttt tgccctcaac
     6661 ggttttactc attgcgatgt gtgtcactga atgataaaac cgatagccac aggaataatg
     6721 tattacctgt ggtcgcaatc gattgaccgc gggttaatag caacgcaacg tggtgagggg
     6781 aaatggctca aatcttcaat tttagttctg gtccggcaat gctaccggca gaggtgctta
     6841 aacaggctca acaggaactg cgcgactgga acggtcttgg tacgtcggtg atggaagtga
     6901 gtcaccgtgg caaagagttc attcaggttg cagaggaagc cgagaaggat tttcgcgatc
     6961 ttcttaatgt cccctccaac tacaaggtat tattctgcca tggcggtggt cgcggtcagt
     7021 ttgctgcggt accgctgaat attctcggtg ataaaaccac cgcagattat gttgatgccg
     7081 gttactgggc ggcaagtgcc attaaagaag cgaaaaaata ctgcacgcct aatgtctttg
     7141 acgccaaagt gactgttgat ggtctgcgcg cggttaagcc aatgcgtgaa tggcaactct
     7201 ctgataatgc ggcttatatg cattactgcc cgaatgaaac catcgacggt atcgccatcg
     7261 acgagacgcc agacttcggc aaagatgtgg tggtcgccgc cgacttctct tcaaccattc
     7321 tttcccgtcc gattgacgtc agccgttatg gtgtaattta cgctggcgcg cagaaaaata
     7381 tcggcccggc tggcctgaca atcgtcatcg ttcgtgaaga tttgctgggc aaagcgaata
     7441 tcgcgtgtcc gtcgattctg gattattcca tcctcaacga taacggctcc atgtttaaca
     7501 cgccgccgac atttgcctgg tatctatctg gtctggtctt taaatggctg aaagcgaacg
     7561 gcggtgtagc tgaaatggat aaaatcaatc agcaaaaagc agaactgcta tatggggtga
     7621 ttgataacag cgatttctac cgcaatgacg tggcgaaagc taaccgttcg cggatgaacg
     7681 tgccgttcca gttggcggac agtgcgcttg acaaattgtt ccttgaagag tcttttgctg
     7741 caggccttca tgcactgaaa ggacaccgtg tggtcggcgg aatgcgcgcg tctatttata
     7801 atgccatgcc gctggaaggc gttaaagcgc tgacagactt catggttgag ttcgaacgcc
     7861 gtcacggtta atgccgaaat tttgcttaat ccccacagcc agcctgtggg gtttttattt
     7921 ctgttgtaga gagttgagtt catggaatcc ctgacgttac aacccatcgc tcgtgtcgat
     7981 ggcactatta atctgcccgg ttccaagagc gtttctaacc gcgctttatt gcttgcggca
     8041 ttagcacacg gcaaaacagt attaaccaat ctgctggata gcgacgacgt gcgccatatg
     8101 ctgaatgcat taacagcgtt agggttaagc tatacgcttt cagccgatcg tacgcgttgc
     8161 gaaattatcg gtaacggcgg tccattacac gcagaaggtg ccctggagtt gttcctcggt
     8221 aacgccggaa cggcaatgcg tccgctggcg gcagctcttt gtctggatag caatgatatt
     8281 gtgctgaccg gtgagccgcg tatgaaagag cgcccgattg gtcatctggt ggatgcgctg
     8341 cgcctgggcg gggcgaagat cacttacctg gaacaagaaa attatccgcc gttgcgttta
     8401 cagggcggct ttactggcgg caacgttgac gttgatggct ccgtttccag ccaattcctc
     8461 accgcactgt taatgactgc gcctcttgcg ccggaagata cggtgattcg tattaaaggc
     8521 gatctggttt ctaaacctta tatcgacatc acactcaatc tgatgaagac gtttggtgtt
     8581 gaaattgaaa atcagcacta tcaacaattt gtcgtaaaag gcgggcagtc ttatcagtct
     8641 ccgggtactt atttggtcga aggcgatgca tcttcggctt cttacttcct ggcagcagca
     8701 gcaatcaaag gcggcactgt aaaagtgacc ggtattggac gtaacagtat gcagggtgat
     8761 attcgctttg ctgatgtgct ggaaaaaatg ggcgcgacca tttgctgggg cgatgattat
     8821 atttcctgca cgcgtggtga actgaacgct attgatatgg atatgaacca tattcctgat
     8881 gcggcgatga ccattgccac ggcggcgtta tttgcaaaag gcaccaccac gctgcgcaat
     8941 atctataact ggcgtgttaa agagaccgat cgcctgtttg cgatggcaac agaactgcgt
     9001 aaagtcggcg cggaagtgga agaggggcac gattacattc gtatcactcc tccggaaaaa
     9061 ctgaactttg ccgagatcgc gacatacaat gatcaccgga tggcgatgtg tttctcgctg
     9121 gtggcgttgt cagatacacc agtgacgatt cttgatccca aatgcacggc caaaacattt
     9181 ccggattatt tcgagcagct ggcgcggatt agccaggcag cctgaatgaa caacgggcaa
     9241 taaatagcca aatctttctt tatcaaaacg tcggcacatt g
//
LOCUS       DAJTRD010000132         9259 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTRD010000132 DAJTRD010000000
VERSION     DAJTRD010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.132"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(70..972)
                     /locus_tag="OO315_003621"
                     /pseudo
     CDS             complement(70..972)
                     /locus_tag="OO315_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            1032..1310
                     /locus_tag="OO315_003622"
     CDS             1032..1310
                     /locus_tag="OO315_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892386.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1311..1838)
                     /gene="gadE"
                     /locus_tag="OO315_003623"
     CDS             complement(1311..1838)
                     /gene="gadE"
                     /locus_tag="OO315_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5892387.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2637..3209)
                     /gene="hdeD"
                     /locus_tag="OO315_003624"
     CDS             complement(2637..3209)
                     /gene="hdeD"
                     /locus_tag="OO315_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5892388.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3464..3796
                     /gene="hdeA"
                     /locus_tag="OO315_003625"
     CDS             3464..3796
                     /gene="hdeA"
                     /locus_tag="OO315_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5892389.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3912..4238
                     /gene="hdeB"
                     /locus_tag="OO315_003626"
     CDS             3912..4238
                     /gene="hdeB"
                     /locus_tag="OO315_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5892390.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4302..4948
                     /locus_tag="OO315_003627"
                     /pseudo
     CDS             4302..4948
                     /locus_tag="OO315_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4990..5520)
                     /locus_tag="OO315_003628"
     CDS             complement(4990..5520)
                     /locus_tag="OO315_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5892391.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5676..6242)
                     /gene="slp"
                     /locus_tag="OO315_003629"
     CDS             complement(5676..6242)
                     /gene="slp"
                     /locus_tag="OO315_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5892392.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6489..7712)
                     /locus_tag="OO315_003630"
     CDS             complement(6489..7712)
                     /locus_tag="OO315_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892393.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8293..>9259)
                     /locus_tag="OO315_003631"
     CDS             complement(8293..>9259)
                     /locus_tag="OO315_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5892394.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2700 a         1845 c         1928 g         2786 t
ORIGIN      
        1 tgaatcccat caaatcccgt ctttttttac ccattcccgg ttatcttgaa taaattacct
       61 agtcataaat acattctggc ggctaccttc atccactaat gccgtgacat acatgccagg
      121 cagcaagtca ccatttgggt tggggaaaat cgcccgtaac gtcacggagc ccgtggtttc
      181 atcaaccgtc gggtcggaga atttcagcgt gccggtctgg ctgtaacgtt taccattttc
      241 cagattgagc tgtactggcg tactgccctg aacctgtttg atttgcccac tggcgacctc
      301 ttctttcatg cgtaagaaat cttgcaccga ctgcgtgaga tcgacataaa tcgggtccag
      361 acgttgtacg gtaaccagcg aatctgcctg attagcggta acgagtgcgc cgacggtcac
      421 cgacgatttc ccgctgacgc ccgtaatcgg cgaggtgaca ttcgcgtatt gcagattgat
      481 cgtcgcctgt tcaacagccg ctttggcgac ggtgacattg gcttctgctt cattcagctg
      541 ggtgcgcgcg gtgtcgtaat cctgacggct aacgtagttg gtcttcagca acgatgcctg
      601 gcggttaaag gtgatgcggg cattgctggc ggtagagagc gctttcgcca gcgagccttt
      661 ggcggagttt agctcggcct gtaaaggtgc aggatcaatc tgatacagcg aatcgccctg
      721 gttcacttta tcgccttcga taaagttgcg tttaatgata ataccgccca cctggggacg
      781 tatctcggca acttcataag gaacggttct accgggcaat tcgctcaaca cattgaccga
      841 accaggggag agtgtgacga caccgacctc aggcgtcatg gcggcggcgt tttccgccga
      901 tttgtcatcg caggcggtga gcatcgcgcc gcagaataac aacggtatta acagctttct
      961 tcttctgttc attttagtcc ctgaaaattc ttgagaaata acggttcttt aaattgcgcg
     1021 cgaggagaga tatgagggat ttacagacca gcggcatagt ggggttgtca gccagtaagg
     1081 tgggatacag gcacagtgat cgacatggtg aggtcaacga caaaaaaaat gttgccgttc
     1141 tgccaacagt tcctgccagc attcgggcaa cgaagggttc tactggtgga tacacatacc
     1201 aggggaatca agtcgtttta gcctctcctt tagtaatcac cgggggtaat gaaatcagca
     1261 tctgcatccc tcgtcatgcc agccatcaat ttcagttgct tatgtcctga ctaaaaataa
     1321 gatgtgatac ccagggtgac gatgtcgctc atacgtctta gtttcagctt gtacataatg
     1381 ttgaatttct gaatctttaa tgtttgttgc gaaatattga gcgtcgacgt gatattgctt
     1441 tcatttttat ttttcagaat cagttgcata attttgaact gattatgtga aaaacataat
     1501 tgtgaattct tatggggcaa gtgtttacca taagtaatct caagaagatt tttgatactt
     1561 tctttgcggc taacaaaata aataggcgct ttagctttta gtttactgat gtgataaggt
     1621 gccaaaacaa taattctctc ggcatctaat ttctccagaa atttaatcgc ttcttcatca
     1681 aggatatgat tgtgataggt atcaatgata accttgaagg gtttattgcc tacttttttg
     1741 atctctgaca gggaattgat ttttatcatt tcgtgattat ctttcaactg ccaaaagccc
     1801 tgtaaaagaa aagaatcttt cgtcatgaga aaaatcataa cttgctcctt agccgttatc
     1861 gtttcgaaca tgtcatcctt ttcattattt acatccttgt ccgaatcgtt gttcaatata
     1921 gtaaacgcct ataagaactt tgcccttcgt gaactaatac gtctattaac actaatcaat
     1981 acttcagatt tatccatcac gcactccata tattgatcct aagtaagatt cctggttgtt
     2041 atcagcttgt aaatagtaaa caaaaagtta acgtttgcgg attactgccc aagaataagt
     2101 tatcaataac cagtttgtga tctctgaaga atattactaa agttaaaatg taatccgatt
     2161 taaatatcga gtctccttgt ttcgacttaa gctggcaatt ggattgccag ctttctttta
     2221 acgcattgaa attacctgag taaataacgc attcatgtct tcaggttttt atatagcgtc
     2281 tggagacacg gatatttatg caatgtaatc aacgccagtt gcaaaaaaat gaacccggat
     2341 tacttattta accgcccata gcatcaacgc ttacggaact ctttgttcac tttcccacag
     2401 cacttttaaa acattaaacc tgactacgga aaatatcagc catgataatg tttgaaatgg
     2461 ctaatttgcc atggagtgaa aaaaattaga tgaaattcag taggttgaaa taatcactag
     2521 caggtaatta tttcaatgat agtgcgcaat tgatctacaa cactgcgtag cggagagagt
     2581 attaatcgga tcatagtcac atcaagtgac tatgatccgg gtgacaaccg gggtaattat
     2641 tgctgcttaa cgaacaaact ggcgaagctg aacaggctgg cggcgctaaa tatcagttca
     2701 attcccacca gtgtggaaac cagcgttaca gacaccatcg gcgttgcacc aaggaatatc
     2761 caggcaatga cgatatccag cacaccaata acgagctgta gccagctgcc tttcattgaa
     2821 cgctgacgat accaactcat caggcgaata acccctgcaa cacagaacaa accggcaata
     2881 aatgccgcaa tggcaaaaat gcccagctcc ggtgcacgga tgaagaaata gccgatcaat
     2941 aaataggcga ctgcgacgag gaaaccggat aataccggcc agaaattatg actgcggttg
     3001 ctgaataacc cgacaataag cgcaataccc gagcagatta ataatgcacc cactactgtg
     3061 cttaaaatat cgccagagac gaacgggaaa ctgatacaca gcaacccgac gataaacagc
     3121 agcacggcaa taaactggat tgctctgcga tgctttttca gcatctccag atcaaacttc
     3181 aaaattgttg ccttatctat atataacata gaaccaccct ataaaattaa gaagaaaatc
     3241 ccctgctatc aatctatgcc aaaaacgcgt ctaagaatgc agtcgattta ataaaaattt
     3301 cctaattgca gtatctgatg catctgtaac tcattgtatt gaaataaaaa tatctgattt
     3361 tgatattttc catcaacatg acatatacag aaaaccaggt tataacctca gtgtcgaaat
     3421 tgattcgtga cggctctttc actttatagt tgaggatatt acgatgaaaa aagtattagg
     3481 cgttattctt ggtggtctgc ttcttctgcc agttgtgagc aatgcagcgg atgcgcaaaa
     3541 agcagctgat aacaaaaaac cggtcaactc atggacctgt gaagatttcc tggctgtgga
     3601 cgaatccttc cagccaactg cagttggttt tgctgaagcg ctgaacaaca aagataaacc
     3661 agaagatgcg gttttagatg ttcagggtat tgcaaccgta accccagcta tcgttcaggc
     3721 ttgtactcag gataaacaag ccaactttaa agataaagtt aaaggcgaat gggacaaaat
     3781 taagaaagat atgtaattcc gggaatgcgt tacatcgtac ttccttgcat attgaacagg
     3841 ccggaatatc ttctttaaaa gcagctattc ctcctgttca tatataatct ctatattgaa
     3901 tgggttacaa aatgaatatt tcatctctcc gtaaagcgtt tatttttatg ggcgctgtag
     3961 cggctttgtc actggtgaac gcacaatctg cgttggcagc caatgaatct gctaaagata
     4021 tgacctgcca ggaatttatt gatctgaatc caaaagcaat gaccccggtt gcatggtgga
     4081 tgctgcatga agaaacagta tataaaggtg gcgataccgt tactttaaat gaaaccgatc
     4141 tcactcaaat tcctaaagtg atcgaatact gtaagaaaaa cccgcagaaa aatttgtata
     4201 ccttcaaaaa tcaagcatct aatgacttgc cgaattaatg aggtacaagt aaaaaggagt
     4261 agcaagttga gccatcttgc tgctcctttt tgcattttta tatgacagca gaatttatta
     4321 tacgtcttat actcgcggca attgcctgtg gcgctattgg catggaaagg caaatgcgcg
     4381 gcaaaggagc agggttacgc acacatgtat taattggcat gggaagcgcc ctgtttatga
     4441 ttgtttcgaa atatggtttt gctgacgtgc tgtctttaga tcacgtcgga ctcgacccca
     4501 gccgtattgc tgctcaggtg gtgacgggcg tcgggtttat cggtgcaggt aacattctcg
     4561 ttcgtaacca aaatattgta ggtctgacga cggcagcgga tatctgggtg accgccgcat
     4621 aggtatggtt attggcagcg gtatgtacga actgggtatt tatggttcag tgatgacctt
     4681 gttggtgctg gaagtcttcc atcaattaac cttccgcctg atgaacaaaa attaccattt
     4741 acagctgaca ttagtgaacg gtaatacggt atcaatgctc gactggttta agcagcaaaa
     4801 aataaaaact gatttagttt cgttgcagga aaatgaagat catgaagtgg tcgccattga
     4861 tatccagctt catgccacaa cgttgataga agacctgttg cgattattaa aaggtatggc
     4921 aggggtgaaa ggcgtttcaa ttagctaatc tgtgtggcag cgtagccaga ctcaccgtaa
     4981 gcctgaaatt cacaccagat aatcaatatg ctgatggcgt accagctcat tgatccgctt
     5041 aagattgagg atcatcatga tattgtgttt atggcagtag aacgttttta gcgatatttt
     5101 cagtttctcc agaatatctt cagttgtcat ttgattcgca atgcagctca gaacctgatg
     5161 ttgcttgcgc gaaagtgaca cattcgcttc aggcacagag cagtaactgc cattgatgag
     5221 tgcgacaaaa tcaatgatag tgatagtccg cggaacaaag gtcaccggta ctttccccag
     5281 acagcgttta atattaaagg gggagaaaac aatgacatgg acaggtttta atcgttcaat
     5341 ttgagtgaga aaattagaat gaaatacatt attcattaat gtatcaataa tgacgaaggc
     5401 attctgatgc aacattacgt cgatctcttc ttcgttctgt atatgaacga cattaccttt
     5461 actcagaatg tttttcatcg cggtgaagaa catcgtatcc ctggtaatta taagaaacat
     5521 aaccgactcc tggtgcggac attaacttta taatcagttt gccaaaggta attaacagga
     5581 cagggcttat aataataata gttagcttac tgtatattaa ttttatgtaa tacatgatgc
     5641 agatgctgag aaagcatctg catctttcgg tggtgttatt tgaccagctc aggtgttacc
     5701 tgactcaccg cattggtgta gtaaggcgca ccccagcccg gttccggcca gaatgcacca
     5761 tagccgtaat cccacaggtt ataggtggta ttaaccactt ctctcaaatg ccacacctgg
     5821 atgccctgca tattcacttc caggaagtta tacgggactt tattgataaa gcctggttgt
     5881 tcaccctgaa tggtaccgag gatggtaaca aagtgattac gatagttcac tggatcaagg
     5941 aagccgcttt gtctggcgag cagtcggccc tgatagttgg cttcaatatc aggcttcgca
     6001 tagctatcca acggtaatac agcgatttct aacaacgtat ccgttttgcc attgataacg
     6061 ttgataacct tcccaccaaa gcgcgcttgt tgaccaacat ataaccccgg ctggttatga
     6121 acagcaacaa aacttttttg aatatcaggt tggttattgc ctttgatatt ttgcggaatt
     6181 gaactacatg cggcaagcaa aaatgaaagg ctgaggatga gtgcaccttt tgtcatgttc
     6241 atgttactat ccttatcaac aaatattaaa accataatag atatttcttt atagttttca
     6301 tctgattctg aggtgatatg cctgatagta acgtaaacct gtctgatgtt tatatccctt
     6361 ctgtttagtt caattccgtt attttggggc gtggtatgag tatccatcgc ccaataatat
     6421 aaccctttgt tttaaaatta cataaaatct atttttattc aatgattata aagtgctaat
     6481 aatcaatatt atggtattat attctgataa tgatttgata agcgaattct tttgccagat
     6541 ggtatttcta tttccacccc ataatttata tctttttcat taggcagatc tctctttaaa
     6601 taaaaggggt atgaaatctt ttcgggttga agtgtctcgt aatgaaattc tatgaattca
     6661 tttttagaca ttgttgccca tttttgaatt ttattgtata gagagggtgt tgtaaactct
     6721 tgaagttgtt tataacatat ttttttgaac tcttgcttat attttaatat gtcatcaaat
     6781 gaaagattca ctacctttgc ctctaagaaa tcaattattc tcgaagcaat ttccgctttc
     6841 gcgcgtaaag agttgtcatt aatagttaac aatgtagaaa acaaaccatt gatttgtttg
     6901 tcaagatgtt gttcatattt atgtagtttg gggatgggat tttccttgtg tgaaaaaata
     6961 gtgttaaata aatttgcgtc agtgaaattt atattaagta gatcttcaaa ataaaatttt
     7021 gataaggtta ggactagctt ggacatatct tgccctgtca tgttgaaatc aataaagtcg
     7081 catccagcaa gggcttccat cctattttca tttttactgt gctcaatata cagcttcgcc
     7141 atttttaagt acaagctttg aatgtctctt atatcagttg gtaagtcagc tcgttgccca
     7201 gcaaaatttt tattatcgga agaaacggtt tggacactga atctttcggg tattccttct
     7261 tctacgagaa ttttagttat gattggattg ttgggttctg gattaacgac aacaatgatt
     7321 tgacatacag aatccttatc cttttcgtat ctaatgttat gaatctgatt aagtattgat
     7381 tgtgtctgtt gcgtaagtgg tgtcgaactt atcgtttcaa tgttattaat aagacttaca
     7441 agttgaacca caacttttga gtagtctgca tttgtatatg tggtgttcgt gtggcatgca
     7501 ttaatttgtt ggagttgttg ttgcgctaaa tcaaattgtt cagggttgta tccttctcca
     7561 gagaatgtgg aattaatggg ttgttgttct tcccaattat cacaaggcca cttcggtgtg
     7621 tctggcgttt taacattatc ggtatttgtt gtgcagttat aaatatcgcc gtgatatgta
     7681 tttattatac ctggggtaaa gtcaattgac ataacattcc ttattcatca tctgttttat
     7741 gctaatgttt ttaaattgga taattagaac aggaatggat tttcttctac acacaataaa
     7801 tataatgcgt tatatcttcc tgatataatt acctttaaat tccctttcat ttccacccgg
     7861 taatatttgt gtataaaaaa agggcagtga tagtatcgta aaggaaaacg ttaacaacta
     7921 tcaaaaaacg ctcattttaa aaagttatta tgttctccga tagtgtgtga agtaatattt
     7981 ttgtgatgtt ttagatgtgt aataatgaac caggttaaag tgtatttaaa aatgtgttgt
     8041 attagcgact gaaatctgcc ttttaaaaag gaggagggta ctgagacaaa gagtcattgc
     8101 ttctccgata aaatgtcaga gaagcatgat tacgtggtta ttgtctgttg atgacttgtt
     8161 gaccgagccg tcatcattct caatgcatta gttaaatcat ttgtgatttt taaggattat
     8221 cctgtctatg attttctact tagaaggata tggcactaac tatgcatctt atagtttact
     8281 gctggcatct atttaaaata gcaattgagt caaatagccc atcaagatag ccatacctaa
     8341 tataaccgtc aggaatgcag ctatcatcgg cattctgaac attgatttaa gtaatatcac
     8401 ttccgtcagg cttgcgccgg cactgccgat gattaatgcc attaatgctc ccagacccat
     8461 tccttttgtc atcagaacag atgccagagg gataaccgcc tcagcccgga tatacagcgg
     8521 aataccgaca acggcgctta atgggatggc gaagggatta tctgctcctg catgagcggc
     8581 aatccacgct gagggaataa agccataaat aaaagatcct attaatactc ctaacaaaag
     8641 atatggcagt acatctttaa actgttgcaa cgcatctttg gtggctagtt ttatgggatt
     8701 aatacttact acagctttgg tactgcaaca ggtaactacg gggttctcaa tggctttagc
     8761 cgctggacta caacaactca cttttatggg cgtatatgtc gttcccggcg aagtttttgc
     8821 tggagcacaa caatttgctg atgagctttt actggcaatg atatgacgtt caaaccccag
     8881 agaatccagg ataatactgg caagaacgga gacgccggct gcgataatcg cgtacaacaa
     8941 ggtgactttc caaccaaagg tcatccacat tagtccgacg ataatgggat taagtaatgg
     9001 tgaaacaaat aaaaaagtga gagtcggacc aaacccggct ttcgctgaca acaatccacg
     9061 taacatgggt attgtcgagc aactacagaa cggggtaacg gctcccaaca gagcagccag
     9121 gagatagcct ctcccttttc tggcccccat catctgctgg attttatggt ctggcacctt
     9181 ttgtcttatc aggctgacac cggcactaat cactataaac agcaacgaaa gctcgacggc
     9241 gagaaacaca aacatctct
//
LOCUS       DAJTRD010000133         9242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTRD010000133 DAJTRD010000000
VERSION     DAJTRD010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.133"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO315_003632"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO315_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5892395.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO315_003633"
     CDS             1828..3147
                     /locus_tag="OO315_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5892396.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO315_003634"
     CDS             3445..3486
                     /locus_tag="OO315_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892397.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO315_003635"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO315_003635"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5892398.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO315_003636"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO315_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO315_003637"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO315_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO315_003638"
     CDS             5815..6711
                     /locus_tag="OO315_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5892399.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO315_003639"
     CDS             6708..7682
                     /locus_tag="OO315_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5892400.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO315_003640"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO315_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO315_003641"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO315_003641"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5892401.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2259 a         2257 c         2317 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 ag
//
LOCUS       DAJTRD010000134         9176 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTRD010000134 DAJTRD010000000
VERSION     DAJTRD010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9176
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.134"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..913
                     /locus_tag="OO315_003642"
     CDS             <1..913
                     /locus_tag="OO315_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5892402.1"
                     /translation="EAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPPALV
                     LMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQLQF
                     SEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIPFLA
                     AAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVAFIS
                     MALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTTHSF
                     NLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1165..2082
                     /gene="oxyR"
                     /locus_tag="OO315_003643"
     CDS             1165..2082
                     /gene="oxyR"
                     /locus_tag="OO315_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5892403.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2065..3465)
                     /gene="sthA"
                     /locus_tag="OO315_003644"
     CDS             complement(2065..3465)
                     /gene="sthA"
                     /locus_tag="OO315_003644"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5892404.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3786..4436
                     /gene="fabR"
                     /locus_tag="OO315_003645"
     CDS             3786..4436
                     /gene="fabR"
                     /locus_tag="OO315_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5892405.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4436..4795
                     /locus_tag="OO315_003646"
     CDS             4436..4795
                     /locus_tag="OO315_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5892406.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4835..5935)
                     /gene="trmA"
                     /locus_tag="OO315_003647"
     CDS             complement(4835..5935)
                     /gene="trmA"
                     /locus_tag="OO315_003647"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5892407.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6049..6239
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6304..8148
                     /gene="btuB"
                     /locus_tag="OO315_003648"
     CDS             6304..8148
                     /gene="btuB"
                     /locus_tag="OO315_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5892408.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8093..8950
                     /gene="murI"
                     /locus_tag="OO315_003649"
     CDS             8093..8950
                     /gene="murI"
                     /locus_tag="OO315_003649"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5892409.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2101 a         2222 c         2478 g         2375 t
ORIGIN      
        1 cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg cacaaaaaga
       61 acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg ctatcgcgcc
      121 tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg ttatcatcgg
      181 cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc gggcgcagtt
      241 tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc gtccacagct
      301 gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga tgattttagg
      361 tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg gttatttgca
      421 agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc ctttcctcgc
      481 ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga aaaaaggcta
      541 cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt cggcattagg
      601 cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta tctcaatggc
      661 gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg tgatggtgtc
      721 agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg tttttgcttc
      781 ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta atctggcgct
      841 ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta ttgtcaaaga
      901 tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga ataaaaaaaa
      961 gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt tgagaaactc
     1021 tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct gtgagcaatt
     1081 atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat cgccatgaac
     1141 tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct ggtggcattg
     1201 gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca gccgacgctt
     1261 agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga gcggaccagc
     1321 cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg taccgtgctg
     1381 cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat gtccggaccg
     1441 ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat tatcccgatg
     1501 ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac ccaccagtta
     1561 ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt gaaagagagc
     1621 gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat ctatgaagat
     1681 cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga aaaactgctg
     1741 atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt tgaagccggg
     1801 gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa catggtggcg
     1861 gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg caaacgcgat
     1921 ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg cctggtttat
     1981 cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat ccgcgcaaga
     2041 atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta acgcagctac
     2101 ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt actcaatagt
     2161 gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt cggcagcgcg
     2221 ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca aaattttcag
     2281 cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact gggcgcggcc
     2341 cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca cagagctgat
     2401 ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg tggcttcgcc
     2461 ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg ccaggctcgg
     2521 ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat acatgctgtt
     2581 gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg ccagcgaatc
     2641 ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt ttttacccga
     2701 cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact cttcgttgtg
     2761 acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca tctcttgatc
     2821 gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat ccataccgcg
     2881 gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta catggcgcgg
     2941 ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg tgaaatcaac
     3001 atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag cggttagtgt
     3061 ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa agcgagcgtt
     3121 cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc gcgtttgttg
     3181 attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga gcagtcggga
     3241 atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg cgtgacggag
     3301 agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt gataacgctc
     3361 gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt cgccgccggg
     3421 gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag ggcttacctg
     3481 ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc aattgatccc
     3541 cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt acttttgccc
     3601 tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg tttccgctgt
     3661 acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc acaatactgg
     3721 agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc aaggattcag
     3781 acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg ctggtggaag
     3841 ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg cgtgaagtgg
     3901 cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac gtagacgaac
     3961 tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg cgccaggcgc
     4021 gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca tttatggagt
     4081 tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc ggcacctccg
     4141 ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa cttgcggact
     4201 atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc gaagcaatgg
     4261 tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa caacgtcggc
     4321 aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct tattactggt
     4381 atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac gagtaatgaa
     4441 acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt tatcgattaa
     4501 tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc cgattatttc
     4561 cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca tgtcggtagg
     4621 cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca gcacggtagt
     4681 tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct ccgtcattat
     4741 ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg agtaatcggt
     4801 atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt aatacgccgc
     4861 actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg accttgtgcg
     4921 tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag atatacagaa
     4981 tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg cgcggtgggt
     5041 cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg cggttaaact
     5101 cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata atttgtacgt
     5161 tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt ttggcgattt
     5221 cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta ccgttgccgc
     5281 agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc cattccagca
     5341 tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga cgatagatca
     5401 tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc gcgattttgg
     5461 ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt gcatcgcgca
     5521 gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc agggaaacca
     5581 ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg cgcagaacgg
     5641 gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt tcactggcgg
     5701 cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg atgtgataca
     5761 ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga ctgaccggcg
     5821 agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc aaacgtacca
     5881 ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg gtcatgatgt
     5941 atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg tccagatctt
     6001 gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct gtagcatcca
     6061 cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga cgcgcagcgg
     6121 taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga agtcatcatc
     6181 tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt cgcatctggt
     6241 tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg tggatgcttt
     6301 acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt ttccgcttgg
     6361 gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga acagccgcgc
     6421 agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg ctggcagtcg
     6481 acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca aaacggcggt
     6541 tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt gctggtgtta
     6601 attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct tagccagttc
     6661 cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc tgtttatggt
     6721 tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc cggaacggaa
     6781 atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac gcagcaacaa
     6841 ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca tggttatgat
     6901 gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg ttttttaagt
     6961 aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg ctttgttcgc
     7021 ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg ttcaccgttg
     7081 ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta taacggcgaa
     7141 ctgattaaat cacaactcat taccagctat agccatagca aagattacaa ctacgatccc
     7201 cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata caccgtccag
     7261 tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga ctggcagaaa
     7321 cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg taataccggc
     7381 atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc agcacgcagc
     7441 gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg ttgggaattc
     7501 atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact aaatctgggg
     7561 caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa acagtgggaa
     7621 ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata tcgtaacgat
     7681 gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga agggaaagcg
     7741 cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac gcatactgtg
     7801 agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt acgccgtgct
     7861 aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg gggtattact
     7921 tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta tcaaaccgtt
     7981 aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac ctctcacctg
     8041 acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt ctatggctac
     8101 caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac cacgtcccac
     8161 cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga tccggcatct
     8221 cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt atggcgaaaa
     8281 aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc aagaacgtta
     8341 tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac ttcctgcatt
     8401 acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac ctgctgcacg
     8461 tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta aacgttctta
     8521 tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc tgggctcggc
     8581 agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc tggatgcact
     8641 aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg ttgtattggg
     8701 ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag agggaacccg
     8761 gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag aacatgaagc
     8821 cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga cgccagaagc
     8881 tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa aactggcagt
     8941 tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt tcctcttgtc
     9001 aggccggaat aactccctat aatgcgccac cactgacacg gaacaacggc aaacacgccg
     9061 acgggtcagc ggggttctcc tgagaattcc ggcagagaaa gcaaaaataa atgcttgact
     9121 ctgtagcggg aaagcgtatt atgcacaccc cgcgcagctg agaaaaagcg aagcgg
//
LOCUS       DAJTRD010000135         9104 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTRD010000135 DAJTRD010000000
VERSION     DAJTRD010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.135"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            18..869
                     /gene="hchA"
                     /locus_tag="OO315_003650"
     CDS             18..869
                     /gene="hchA"
                     /locus_tag="OO315_003650"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5892410.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(977..2335)
                     /gene="hprS"
                     /locus_tag="OO315_003651"
     CDS             complement(977..2335)
                     /gene="hprS"
                     /locus_tag="OO315_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5892411.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2335..3006)
                     /gene="hprR"
                     /locus_tag="OO315_003652"
     CDS             complement(2335..3006)
                     /gene="hprR"
                     /locus_tag="OO315_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5892412.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3139..3552
                     /gene="hiuH"
                     /locus_tag="OO315_003653"
     CDS             3139..3552
                     /gene="hiuH"
                     /locus_tag="OO315_003653"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5892413.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3661..4665
                     /gene="msrP"
                     /locus_tag="OO315_003654"
                     /pseudo
     CDS             3661..4665
                     /gene="msrP"
                     /locus_tag="OO315_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4666..5301
                     /gene="msrQ"
                     /locus_tag="OO315_003655"
     CDS             4666..5301
                     /gene="msrQ"
                     /locus_tag="OO315_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5892414.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5559..6209
                     /gene="zinT"
                     /locus_tag="OO315_003656"
     CDS             5559..6209
                     /gene="zinT"
                     /locus_tag="OO315_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5892415.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7287..7556)
                     /locus_tag="OO315_003657"
     CDS             complement(7287..7556)
                     /locus_tag="OO315_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5892416.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7613..8281
                     /locus_tag="OO315_003658"
     CDS             7613..8281
                     /locus_tag="OO315_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5892417.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8597..>9104
                     /locus_tag="OO315_003659"
     CDS             8597..>9104
                     /locus_tag="OO315_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5892418.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARN"
BASE COUNT         2603 a         2051 c         1865 g         2585 t
ORIGIN      
        1 acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc gatattgctg
       61 aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt cccgtctctg
      121 atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt ctggtgatcg
      181 ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc ggtaaccatc
      241 cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa ttcgaagtgg
      301 cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac aaagatgaaa
      361 aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag aaactcgcgg
      421 atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt cctggtgggc
      481 atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta cagtgggcaa
      541 tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt ctggcacttc
      601 gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac gccgcagaca
      661 aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc ggcgaagagt
      721 tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta cataaggacc
      781 gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa ctggcggcgc
      841 aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta agcgcataat
      901 caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt aacttatggc
      961 gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg ttatgcttac
     1021 tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg actaaagaaa
     1081 ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat ctacggaaga
     1141 gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta agagagccgt
     1201 tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat cgaatggcgt
     1261 taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag atttgttgat
     1321 tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga tagtccaaaa
     1381 gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca ttgtttttgt
     1441 ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc agctcttcaa
     1501 tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg agcgtaacct
     1561 gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga tcgtcagcaa
     1621 attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc aacgcctgcc
     1681 caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg ctatcgttat
     1741 aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc gtcctgatta
     1801 acaacggact taatactgag cacagtataa tggcgacaat gcaaattata atgctattaa
     1861 ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca gtaaccgtta
     1921 atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta tcattaataa
     1981 tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta tttaacatgt
     2041 catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga atgatcaaaa
     2101 tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct ggatttaccc
     2161 catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta tcatcgcgcc
     2221 atttcaactc acttgccagg ccattataga gagtccatac aattccagcg ccagcaacag
     2281 actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt ttcattattt
     2341 ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct tttcaggaaa
     2401 aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat tggtatcact
     2461 atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa tctcgccagc
     2521 tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa tactgatatt
     2581 gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt ctaatgttga
     2641 attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact cagaaaatga
     2701 aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc tgtcatcgac
     2761 agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta acgtttgtaa
     2821 gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg cataatcatc
     2881 cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga cataacccgc
     2941 ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa taagtagaat
     3001 cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt tcattacaaa
     3061 attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa aactcattca
     3121 catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc aacggcagcc
     3181 ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt gcacattttg
     3241 aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa gaaagcggat
     3301 aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat taaggcactg
     3361 tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac cggggactat
     3421 ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt tcatattaat
     3481 aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta ttcaacctat
     3541 cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag ggaggtgact
     3601 ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt gtgatggctt
     3661 atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt attctttatg
     3721 aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc tttgcctcac
     3781 gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc cgcccccgcc
     3841 ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc actgacgcca
     3901 gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa agacgatccc
     3961 gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag cggcgaagtg
     4021 gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct ggaagagcgt
     4081 atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat tggttttccg
     4141 ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt cgctttcgaa
     4201 acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg cggcgggctg
     4261 aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct cacactgatg
     4321 accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt gcgactgatt
     4381 gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa gctgacccgc
     4441 gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt ttacgccaac
     4501 gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt tattggttca
     4561 ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta cgccgaccag
     4621 gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg tctgacagca
     4681 aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc gtttctctgg
     4741 ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga tattcagcat
     4801 tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac acctctggca
     4861 cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt atggtgcttt
     4921 gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt gaataatctg
     4981 gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat tatcagctgg
     5041 gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa actcggcaaa
     5101 cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc aatacattat
     5161 ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct ggctgtactg
     5221 cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg caagcaggcc
     5281 cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat cccggccgga
     5341 tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt tcgtttgtca
     5401 tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca atacacctga
     5461 gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt acaatataac
     5521 attacacata atgtacatta actttggagg aaattgtttt ggcgattcgt cttcataaac
     5581 tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat ggtcatcatt
     5641 ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt tttgatgata
     5701 ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc gtttaccctt
     5761 tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca gataaaacca
     5821 aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat atcgaaatga
     5881 ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca tcctgtaaat
     5941 atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc gttcgttact
     6001 tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt agcgatcata
     6061 taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat tcccagcaat
     6121 ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg agtagcgaag
     6181 aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt tatttaacag
     6241 aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga caaactcaga
     6301 cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac tgagaaaaag
     6361 gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt tccaggagca
     6421 agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt ttaaaccttt
     6481 tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa caactcttgg
     6541 aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt ttggctcaat
     6601 tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc agtttcatag
     6661 gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa ctgtagattt
     6721 tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc acagtatcga
     6781 acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct gtttaggaaa
     6841 agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag ggggatttga
     6901 acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga gtcgaaaaat
     6961 caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga aaaatcaatg
     7021 aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac ctgttttcag
     7081 gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct cagcttattc
     7141 cgttttgcct gtgtgttgaa taattccact gcagttatat aaaatcggta acggctggaa
     7201 atcattcaat actcgcacta tcgaaagttt accagccaac cgcagcacgt cctgcatacg
     7261 tcgtgtctgc ggtttttctt tttcgcttac attgtgtctg gttcttccgg ccactcaata
     7321 tcaggtgcag ttgatgtatc aacacggttc agcaacaccc gatacttctt ccaggcttcc
     7381 agcaacgagt tttcttcctc cgttgcgatc tccagatcta cagcatcctg aagtggcgca
     7441 atatgctcac tgaatccctg gatgtagaac tgtgtggtga cggtcttcca gccattcggc
     7501 tcctgctgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg cagcatttaa
     7561 ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata atatggtgaa
     7621 attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta ccctaaagct
     7681 ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc ctacaaagga
     7741 acaccttctt ccacactttc tggaacattt aggtaacaac catctggata ttggtgttgg
     7801 aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa tggatttgaa
     7861 cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta aacataaaat
     7921 tagccatgat gtttttgaac cttatcctgc ggcgttacat ggtcaatttg attccatttc
     7981 catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct gtgtaatacg
     8041 caatgcggcg caggccttaa ctgatgatgg aactctatac ggagccacaa ttcttggcga
     8101 tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc agaaaggcat
     8161 cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag agcatttcga
     8221 gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag ggaaaaaata
     8281 gcatccaacc gcagcacgtt cttgcttaag acgtgctacg gtttccttta cgatttgggg
     8341 ttgcgacttt acctctattg ataatgcatt ccggcagaac gttcaaatat gaacgtacga
     8401 tatttaacta accgaaaaac aaaatatagc acaggcgaga catttaccat ctaagaaaat
     8461 agtatcgttt ttttacagtt aaatcaacat cacttcctta aaatgaaaac aataaatttc
     8521 aatcctgaaa cattttattt cagcttatta ttccccagta tcacatttta tcaggatatc
     8581 cttctgcgag gttattatgc ttcctgtaaa taatcccccc ctatccactg gaaacgtctc
     8641 tttttacaga actacatcaa tcgacaatgt tcacaataat tatctctccg aatgggttga
     8701 atggactaaa aacagcattt ccggagaaaa cagggaaact gcttttaccc ggctccaatt
     8761 atgtctggag aacagtgaaa catcgttgga cttatcttgt ttaggtctca gatctctacc
     8821 acgattgcct gacaatcttg atgaaattaa tgtaagcaat aaccaactat caatgctccc
     8881 cgagctacca agggcattga aagagctgaa tgcaagcagt aatcaattat ctgcacttcc
     8941 tgaattacca gtgtcgctgg aatatataaa tgtgagtgat aaccatttgt tcgcacttcc
     9001 tgaattacct gcgtcactag aatatattaa tgtaagtgac aatcacctgt ctgtacttcc
     9061 gaggttacca atgtcattgg aattacttga tgcagccaga aatg
//
LOCUS       DAJTRD010000136         9029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTRD010000136 DAJTRD010000000
VERSION     DAJTRD010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.136"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO315_003660"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO315_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5892419.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO315_003661"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO315_003661"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5892420.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO315_003662"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO315_003662"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5892421.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO315_003663"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO315_003663"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5892422.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="OO315_003664"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="OO315_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5892423.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /locus_tag="OO315_003665"
     CDS             5562..6035
                     /locus_tag="OO315_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5892424.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="OO315_003666"
     CDS             6064..6606
                     /locus_tag="OO315_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5892425.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="OO315_003667"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="OO315_003667"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5892426.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="OO315_003668"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="OO315_003668"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5892427.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="OO315_003669"
     CDS             8003..8260
                     /locus_tag="OO315_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5892428.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="OO315_003670"
     CDS             complement(8236..8790)
                     /locus_tag="OO315_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5892429.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2258 a         2192 c         2253 g         2326 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacagcgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DAJTRD010000137         8985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTRD010000137 DAJTRD010000000
VERSION     DAJTRD010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.137"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(138..2681)
                     /gene="mdoH"
                     /locus_tag="OO315_003671"
     CDS             complement(138..2681)
                     /gene="mdoH"
                     /locus_tag="OO315_003671"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5892430.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2674..4209)
                     /gene="mdoG"
                     /locus_tag="OO315_003672"
     CDS             complement(2674..4209)
                     /gene="mdoG"
                     /locus_tag="OO315_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5892431.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4603..5760
                     /gene="mdoC"
                     /locus_tag="OO315_003673"
     CDS             4603..5760
                     /gene="mdoC"
                     /locus_tag="OO315_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5892432.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5768..7249)
                     /gene="clsC"
                     /locus_tag="OO315_003674"
     CDS             complement(5768..7249)
                     /gene="clsC"
                     /locus_tag="OO315_003674"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5892433.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7191..7724)
                     /gene="ymdB"
                     /locus_tag="OO315_003675"
     CDS             complement(7191..7724)
                     /gene="ymdB"
                     /locus_tag="OO315_003675"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5892434.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7819..8130)
                     /locus_tag="OO315_003676"
     CDS             complement(7819..8130)
                     /locus_tag="OO315_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5892435.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8251..8583)
                     /gene="csgC"
                     /locus_tag="OO315_003677"
     CDS             complement(8251..8583)
                     /gene="csgC"
                     /locus_tag="OO315_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5892436.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDMYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WTPSSEKS"
     gene            complement(8642..>8985)
                     /gene="csgA"
                     /locus_tag="OO315_003678"
     CDS             complement(8642..>8985)
                     /gene="csgA"
                     /locus_tag="OO315_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HCR5892437.1"
                     /translation="SGPNSELNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVG
                     QGSDDSSIDLTQRGFGNSATLDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQV
                     GFGNNATAHQY"
BASE COUNT         2246 a         2327 c         2133 g         2279 t
ORIGIN      
        1 gccggtattt ttattggctc gttgtttttg ccggatgtgg cgtgaacgcc ttatccgacc
       61 tacaaaatct tgcaaaatca ataaattgca ggaacgatgt aggcctgata agcgtagcgc
      121 atcaggcaac tacgttttta ttgcgaagcc gcatccggtt tacgcaatgc cagtggattg
      181 agctttatcc cttcgtaata actcacccat gaagaatatc tttcagggga attccagacg
      241 cggaaatgca gacgggccat cgtcaccgga tcgcttagca gcaccaggcg acgatcgcga
      301 ttcagcttct ctggcgtctc gttaagcgcc tgttcaacgt ggcggtcacg ggcgatttcc
      361 agcaccttac tggcgcggtg acgcgctgtc gccattgcgg ttgccagagc gttaaatgac
      421 gggttaaaca ctgcgtgcat aaagccatcg tcaagggagc gttggcgatt catctcaagg
      481 aaccgatcgg tatcaaccag cacctgcggc ggtgaatact cttccgggat caggaacagt
      541 ttccagcgtt tggtgcgcag accaacggtg gcacggctgg aaatcaccga aacaaacggt
      601 gacaggatca acgagaagac aatcggtgcc agccagaaca ggaaacgcag atccagccac
      661 gccatcccaa cagcccacac taaccccaac agcagctgtg agccgtggcg tttgaacgct
      721 tcgccccatg aagtggagtc atcatcacgc tgcggtgaat tccacaccac ttcccagcca
      781 aggaacgcgc tgacaacgaa gaccgtatgg aacagcatgc gtaccggagc cagcagcacg
      841 gaaaacagca cttccagcag caacgataat gtaacgcgcc agaagccgcc gtattctttc
      901 gttcctttgc accagataag caaaatgctc aataacttcg gcaggaacaa cagcaccatg
      961 gtcgaagcaa aaagtgcaat cgccagctca ggacgccact gcggccacac cgggaacaac
     1021 tgccgtggtt gcaggaagta ttgcggttcg gtcaacgcat gcactacctg caatgcagta
     1081 gagagcgcga ggaacataaa ccacagcgga gcggagagat aagacatcac gcccgtcagg
     1141 aacaccgcac ggtgaaccgg gtgcataccc ttaaccagga acagacggaa gttcatcagg
     1201 ttaccgtggc accagcggcg gtcacgtttt agctcatcaa gcaagttagg cggcaattct
     1261 tcataagaac ccgggagatc gtaagcaatc cagacccccc aacctgcacg gcgcatcaac
     1321 gccgcttcta cgaagtcatg tgacaggatt gaaccggcaa aggaaccttc gcccggcagc
     1381 ggagccagtg cgcagtgctc gataaatggt ttcacgcgga taatcgcgtt atgtccccag
     1441 tagtgcgact cgccaagttg ccagaagtgc aaaccggctg taaacagtgg cccatacacg
     1501 cgggtcgcga actgctgaca gcgcgcatac agcgtatcca taccggacgc tttcggcgac
     1561 gactgaatga tcccggcgtt cgggttggct tccatcaagc gcaccaggcc gcataaacaa
     1621 tcaccggtca ttaccgagtc agcatccagc accaccatgt agctgtactg gctgccccag
     1681 cgacggcaga agtcatcgat attaccgctt ttacgcttca cgcggcgacg gcggcggcga
     1741 tagaaaatct gaccttcgcc accgacttca gcgataagct ccatccaggc tttttgctct
     1801 gcgacgcaga tatccgggtt ataactgtca ctcagaatgt agacatcaaa gtgtttggca
     1861 ttcccggtgg cttttactga ttcccacgtt gcacgcaagc cagcaaaaac acggttcacg
     1921 tcttcgttac agataggcat gatcaacgcc gtgcgatgct ccgggtttaa tggttcatcg
     1981 ccaactgttg acgcagatat actgtattta tcgcgaccaa taagcagttg caggaagccc
     2041 atcaacgccg tccagaatcc ggcggacacc caacagaaca gtaccgcaaa aaggatcagg
     2101 ataccggttt gcagcatata aggcagaagc tgcataaagg aaacccacaa atcctgacca
     2161 accatatcca taggattaat cagcgcccaa ccctgataag gaagaatggt cttcatatac
     2221 caggtcgcga caacagtttg cgcgagcgtc aggatcaaca gaatgtaacg gcggatggta
     2281 ccaacggtac gccacttttg ctcgctctcc tgctcttctt tggtcaaacg agccagatag
     2341 cgcggcgtga catcgcgtcc acgcaggcga tcccagaaac ggcctaccgg gttggtacgc
     2401 cacgggtcgg gaaacatcga ggagcgtttt gcttctggca tcgccttaag ctgatcgcgc
     2461 ccttcgtcgt ctttaattaa ctgtccatca gcaagtgaat ctggccaggc ttgttccaga
     2521 cgcgccttta ccgagccttg cggggaatca tcctcccgcg cccaggtgcg gtgttcggca
     2581 tccagcgcct gatgaacggc gcggatatca gtcttcggca atgctgcttt ctcgcttgcg
     2641 gcgatgggca ttgcgtcaat gtactcagtt gtcttattca ttggcaggta actggtagct
     2701 ccaggtttca ctcaacgtct gatcggcatt caccagcgca gcacgcattt cagtgatttt
     2761 cttggcatct ttcactttca cacgcatcac cagacgccag cctttggtaa ccgggttata
     2821 gcgcaccgtg ctttcaacta tctcaccatt atcaccaatg ctggtttgcg ctgtgaccgg
     2881 ggtatcctct ggcagttttt tcatctctgc gccggtaaaa tcgaccacaa aggcgatagt
     2941 accgtcaggc tggcgaatca ggttcgactg cttcacatcc cccgttgaac gacgcgtttg
     3001 ttgcacccat gcgttatctg gcgcatgcag tttgtcttca tcacggctga aggtgatggt
     3061 gtatttaaag ttcatctctt tacccggctc cggcagctga tccggcgtcc agtaagcgac
     3121 gatgttatcg ttggtttcat cgttggttgg aatttccacc agctcaacgc tgcctttgcc
     3181 ccactcccct ttcggagtca cccatgcgct tggacggaga tcgtaacgat catcgagatc
     3241 ttcaaagcgg gagaaatcac gacctcgttg caacagacca aagcctcgcg ggttttccat
     3301 ggagaagctg ctgaccgcta aatgtttcgg gttattcaac ggacgccaga tccattcgcc
     3361 attaccagca tggatagaca gaccgttaga gtcgtgcaac tccggacgat agttatttgc
     3421 aggcgacggt tggttcggcc caaacaggaa catactggtt aacggtgcaa cccccagttt
     3481 gccgacttta tcgcgcagat agattttcga ctgcacatcc acaaccgtgt cacgccctgg
     3541 cataaccacg aatttgtaag cacctgtcgc acgcggcgag tcaagcaatg cataaatggt
     3601 taaacgttta tcagtcggtt ttggacgctc gatccagaac tctttgaagc gtggaaattc
     3661 ttcacccgat ggcaaagcgg tatcaattgc taggccgcgg gcagaaaggc cataaacctg
     3721 acctgcacca atcacgcgga aatagctggc cccgagcatg ctgacgattt catcgttttt
     3781 atctttgctg ttgatcgggt aaagcacttt aaaaccggca aaaccaaggt cttttaccgt
     3841 gtctttgtca tgctgaacat cgccgaaagt gaaataatcc gggctgtatt tgattcgttt
     3901 gactgcggtg gcagtcactt catttatttt gaccggggta tcgaagtaca taccctgatg
     3961 gtagaactcg agtttgaatg gggtcttcag attgttccag tacgctttgt catgattaaa
     4021 ctggatctgc tgatagtccg cgtatttcat gtcgcggaaa acggagggca agttgctttt
     4081 aggcgcctca tagcctttcc cggctaagga ttgagcttgc tttgcgacat catcaatact
     4141 gaaagcccag cttgaagatg tatacagggt taacattact gcagcactca accaacgcat
     4201 tttcatcatt tgtagtttat gtttcataat tagtaagcac ttcccccttt gtgtgcttat
     4261 atcgatccga tctattttaa tggataacca gccattccga caactgaatc ccggtatcgt
     4321 tcagcacgct ggctcatgga ttaagcacct gattgcaacc gttttcactt tccgtgcttc
     4381 tcctaaagac agaagcatcg acttcgtgta gggtttgcca gcaaatttat ttatcaaccg
     4441 tcttacagac gcttccattc tggttagtgg tagcgatgat gctatgcata aaaaccttga
     4501 agcctgcgcc tggcgcggtc aggtcggtgt cgctatggca acaagcctaa aggcataacc
     4561 cgacagggat aagacgaaaa atccgagatt agttaaccat atatgaatcc agtacccgcg
     4621 caacgtgaat atttcctcga ctccatccgc gcctggctga tgttgttagg gatccctttt
     4681 catatttctt taatctattc gagccataca tggcatgtga atagcgccga accgtcattg
     4741 tggctaaccc tttttaatga cttcatccac tcgttccgca tgcaggtatt tttcgttata
     4801 tccggatact tttcctacat gcttttttta cgctatccct tgaaaaaatg gtggaaagta
     4861 cgtgtcgaac gtgtaggtat cccgatgtta acagccatcc ccctactgac attaccgcaa
     4921 tttattatgc tgcaatatgt caaagggaaa gcggaaagtt ggcctgggct atcattgtat
     4981 gacaaatata atacgttggc ctgggaatta atatcacacc tgtggttttt actggtgtta
     5041 gtagtcatga cgacgctgtg tgtatggata tttaagcgca tcagaaataa tttagaaaat
     5101 tctgataaaa cgaataaaaa attctcgatg gtaaaactat cggtgatttt tttatgcctc
     5161 ggcatcggct atgcggtaat aagaagaacg atttttattg tgtatccacc cattctgagt
     5221 aatggcatgt tcaattttat tgtcatgcaa acgctatttt atttaccgtt ctttatcctc
     5281 ggcgcactgg ctttcatttt ccctcatctt aaagccttgt ttaccacgcc gtctcgtggc
     5341 tgtaccctcg ctgcagcatt ggcgtttgtc gcttatttac tcaaccagcg ctatggcagt
     5401 ggcgatgcct ggatgtacga aaccgagtcg gtgatcacca tggtcctcgg cctgtggatg
     5461 gtgaatgtgg tcttctcctt tggccaccgt ttgcttaact tccagtcagc gcgggtgact
     5521 tattttgtta acgcatcgct gtttatctat ctggttcacc acccgttaac gctgtttttc
     5581 ggcgcataca ttacaccgca catcacctcc aactggcttg gttttctctg tggcctgata
     5641 ttcgtagtag ggattgcgat aattctgtat gaaattcatt tacgcatccc gttactgaag
     5701 tttttgttct ctggtaaacc ggttgttaag cgtgagaacg ataaagcacc agcccgttaa
     5761 gccacattta caataaccat tccacgggca atatcgacgc cagtctgacc ataacccgct
     5821 tccagaaact ggtggcgggt tcttttttga gaataacctc tttctttgca tgacgatcaa
     5881 cccagttgat ccgtccccac ctgtccagac ggagctgcca ggccgcatcg tactggctct
     5941 gaataaagcg tttatcaatt aactgtgcca gcgtttcgct ctctatcacg aagcccattt
     6001 cagtattgag caatgttgaa cgcggatcga aattgaaaga gccgataaac accgttttac
     6061 catcgatgct aaaagtttta gcatgcaggc tggcaccgga attaccggtt atgccgcgat
     6121 cgtgtaacgt actgctttgt tcacgcgtcg gcttgagttc atataattcc acgccatagc
     6181 ggagcaattt tttgcgccag cgcgcgtatc cggcatggac gacagcaaca tcgttagcgg
     6241 caagagaatt ggttaggatc gcaatcttta ccccttttct caccatccgt aagagttgcg
     6301 ccacacctgc gcgtgtcggt acaaaatagg aagagataat atcgatgcgt tcactgggtg
     6361 agcccatgat atcgaacagg cgctgcggta acagtgaatg ccgttttgcc ttgccctccc
     6421 ctttcgccgg atcatcactt aataaacgtg tcttcgccca gataagcggc aacgttccat
     6481 caaccagatg atttataaat ggactggatt ccattttgcg taaataacga tgcgtcatgg
     6541 catcgttatg ccaggaggcg ggtaactcga tgcgatccgc catttcacct tccgggacat
     6601 ccagcacctg ctgtaagggt gaaaccgatt tgcaatacca gtagcgggcg aaatcatcgg
     6661 caacatcctc taccacgggg cctattgcca tgacatctaa atccgaaaaa agtggctctt
     6721 cccctgcacc aaaataggca tcaccaatat ttcgtcctcc cactagggta accacgccat
     6781 cgacagtgaa acttttattg tgcatccggc gattgagacg ggaaaagtcg gtgatatatc
     6841 caagcggacg tagcaagcga aacgagaaag gattaaaaag ccggacttca atgcgtggat
     6901 gactgtcaag caagcgcaaa atgtcgtcaa gtccgggcgt attgttgtcg tccagcaaca
     6961 aacggacgcg aacgccacgc tttgctgcgg ctaacagggc ggaaaacagt aaccgccccg
     7021 acatatcgtc ctgccagatg taatactgaa catccagcgt atgctctgcc atttcggcca
     7081 gacgataccg ggcggcaaac gcatccagac ttttctccag agggaaaaga ccacactgac
     7141 cgggatgttg cgaacacagt ggcagcaccg cgctcgccag ccggggcaaa tcattcatct
     7201 ccttgttggg taaggagtct ttcgtagagg tgggcgtttt cttcatcata acagacaaag
     7261 tatacctgtt cgggtaaagc gtgacgggta ataaattctg aaacggtttt aactgcgatt
     7321 tcagccgctg ccgcacgagg gtaactataa accccagtac tgattgcagg aaaagccact
     7381 gacgtatagc tgtttgccgc caccagtcgt aggctattca gataggcatc ctgcaaaagc
     7441 tggtcttcgt tttgttcccc accacgccag actggcccga cggtgtgtac tacggcttta
     7501 gcgggaagat cgcctgcaag cgtaataacg gcatgccccg tagggcaatc gccctgctgt
     7561 tgcctgactt ttaaacaggc atccagcagg gccggacccg ctgcgcgatg aatggcccca
     7621 tcgacgccgc cgcctcccat taatgacgga ttagccgcat tcacaatcac atcaacggcc
     7681 agtttggtaa tatcaccctg cacaacatga atacgcgttt tcataacgcc tccttacacc
     7741 ttcttgtttc ttaagtgtac agcaggcttt ttggttaaat cgcatttttc tgctcagcaa
     7801 cgtgagttgt tcagaaactt agttatattc gatgttcatt acagccagct ttttctgttt
     7861 atcaagatac tgcactttta ctgaggctat ttgcgcgttt ttgacattgc cgctcattaa
     7921 cgctttgctg gagtaggttt tgcccggaat agaaccattc tgtgggcagt tcatttctac
     7981 gggtgatgac tgggtgctga cgtcacatgc cggttccaca atttggccat aaaaatggat
     8041 cacgccccct tgcgcggtgc ttcctgcaat ggcaataaaa ggaaaaaaca aactgccgag
     8101 cataagagag tctaaaaaag gtctgaacat ttattctatt tcctcaatga ttagtcatcc
     8161 ttgaaggttg tgttatccat actttcaggt tggctgattc gcagcagacc attctctcca
     8221 gattcatctt atgctcgata tttcaacaaa ttaagacttt tctgaagagg gcgtccattg
     8281 ttgtgataaa tgaagtgcct gtccatcaga aacagtaaca actattttca cccgatcttc
     8341 cggggaaata tttaaactca acttcgtcaa agcaatgggt tgattagcag gcaatgagag
     8401 ggtcttttct tgcttcgtct gactttgccc tgaactgcct tcgcgcaggg acaatatttg
     8461 tactctgcac agacaagatt gagtaagagt aacttcagga ataatggtgt acatatcccc
     8521 ttgctgggtc gtattaaagg ttatctggct ggaaagtgcc gcaaggagta ataacgcatt
     8581 catattcttc tcccgaaaaa aaacagggct tgcgccctgt ttctgtaata caaatgatgt
     8641 attaatactg atgagcggtc gcgttgttac caaagccaac ctgagtcacg ttgacggagg
     8701 agttcgatgc agtctggtca actgcagcac cgttgccacc accgaactgt ttaacagtca
     8761 tttcagaatt tttgccgttc cactgatcaa gagtggcgct gttaccgaag ccacgttggg
     8821 tcagatcgat tgagctgtca tctgagccct gaccaacatc tgcaccatta ccaccgccat
     8881 gctgggtaat agtcaagtca gagttacggg catcagtttg cagagcaagt gcagagttac
     8941 cgccaccgta ctggtaaatg ttcagctctg aatttgggcc gctat
//
LOCUS       DAJTRD010000138         8958 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTRD010000138 DAJTRD010000000
VERSION     DAJTRD010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8958
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.138"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            175..522
                     /gene="tnpB"
                     /locus_tag="OO315_003679"
     CDS             175..522
                     /gene="tnpB"
                     /locus_tag="OO315_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5892438.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            572..1957
                     /locus_tag="OO315_003680"
     CDS             572..1957
                     /locus_tag="OO315_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5892439.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(2360..2941)
                     /locus_tag="OO315_003681"
     CDS             complement(2360..2941)
                     /locus_tag="OO315_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892440.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(2988..6845)
                     /gene="sigA"
                     /locus_tag="OO315_003682"
     CDS             complement(2988..6845)
                     /gene="sigA"
                     /locus_tag="OO315_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5892441.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(7206..8210)
                     /locus_tag="OO315_003683"
                     /pseudo
     CDS             complement(7206..8210)
                     /locus_tag="OO315_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            8195..8572
                     /locus_tag="OO315_003684"
                     /pseudo
     CDS             8195..8572
                     /locus_tag="OO315_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(8611..>8958)
                     /locus_tag="OO315_003685"
     CDS             complement(8611..>8958)
                     /locus_tag="OO315_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5892442.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         2175 a         2138 c         2164 g         2481 t
ORIGIN      
        1 cccccagcta cttcctgtga ctctcgatgc agctgccgaa cagccagaat cgctcgcaga
       61 ggacccggaa accctcagta tcagctgtga ggtaacgttc cggcacggga cgctccgctt
      121 caatggcaat gtcagcgaaa agctcctgac tctgctgata caggaactga agcgatgatc
      181 ccgttacctt ccgggaccaa aatttggctg gttgccggta tcaccgatat gagaaatagc
      241 ttcaacggcc tggctgcgaa agtacaaacg gcgctgaaag acgatcccat gtccggccat
      301 attttcattt tccggggccg cagcggcagt caggttaaac tgctgtggtc caccggcgac
      361 ggactgtgcc tcctgaccaa acggctggag cgtgggcgct tcgcctggcc gtcagcccgt
      421 gatggcaaag tgttccttac gcaggcgcag ctggcgatgc tgctggaagg tatcgactgg
      481 cgacagccca agcggctgct gacctccctg accatgctgt aaatctcttt atcctggttg
      541 tcacagaata agcccggtaa aatacgggct tatgaacgac atctcttctg acgacatctt
      601 cctgctgaaa cagcgcctgg ccgaacagga agcgctgatc cacgccctgc aggaaaagct
      661 gagcaaccgg gagcgcgaaa tagaccatct gcaggcgcag ctggataaac tccgccggat
      721 gaacttcggc agtcgttccg aaaaagtctc ccgccgtatc gcacaaatgg aagccgatct
      781 gaaccggctt cagaaagaga gcgatacgct gactggtagg gtgtatgacc cggcagtaca
      841 gcgtccgttg cgtcagaccc gcacccgtaa gccgttccct gaatcactac cccgtgacga
      901 aaagcgactg ttgcctgcgg cgccgtgctg cccgaactgc ggcggttcac tgagctatct
      961 gggcgaggat accgccgaac agctggagtt gatgcgtagc gccttccggg ttatccggac
     1021 ggtacgggaa aaacatgcct gtactcagtg cgataccatc gtgcaggcac ctgcaccttc
     1081 gcggcccatc gagcggggta tcgccggacc ggggctgctg gcccgcgtgc tgacctcgaa
     1141 gtatgcagag cacaccccgc tgtattgcca gtcagaaata tacggccggc aaggtgtgga
     1201 gctgagccgt tcactgctgt cgggctgggt ggatgcatgc tgccggctgc tgtctccgct
     1261 ggaagaggcg cttcatggct atgtcatgac tgacggcaaa ctccatgccg atgatacccc
     1321 ggtccaggta ctgctgccgg gtaataagaa gacgaagacc gggcggttgt gggcgtatgt
     1381 tcgtgatgac cgcaatgccg ggtcagcgtt ggcacctgca gtgtggttcg cttacagccc
     1441 ggacagaaaa agcatccatc cgcagactca tcttgcttgc ttcagcggtg tgctgcaagc
     1501 ggatgcgtac gccgggttca acgagctgta tcgcaatggt gggataacgg aagctgcctg
     1561 ctgggctcat gcccgccgaa agatccacga tgtgcacgtc cgcatcccgt cagcactgac
     1621 ggaagaagcc ctggagcaga tcggtcagtt gtacgccata gaggcggata taaggggaat
     1681 gccggcagag cagcggcttg ctgaacgtca gcgaaaaacg aaaccgctgt tgaaatccct
     1741 ggaaagctgg ttgcgtgaaa agatgaagac cctgttcttc ggctctggcc atggtggtga
     1801 gcggggagcg ctactgtaca gcctgatcgg gacgtgcaaa ctgaatgacg tggatccaga
     1861 aagctacctt cgccatgtgc ttggcgtcat agcagactgg ccggtcaacc gggtcagcga
     1921 actgcttccg tggcgcatag cactgccagc tgaataacac atccccgtca atacggccct
     1981 cgctgtacgc ttactcatca cctgctatta atccccgagt cagtacttcg cggcctgacg
     2041 cgatcaccct actcagtact tcagagcccg cctccggtac ctgcctggcg atcgcccctg
     2101 tgcaacaaaa taaatcaacc gttatcaaac cacgatcgtt cgtatgcgcc gccgatgacg
     2161 cgcgcctgaa aaacgcgtca atatgagttc cccggaagct tacgataata cgcttatgaa
     2221 cattacaaca cgctcatgcg gaacctggat gggggaagga acggtgaagc tgttagcgaa
     2281 tgaccggcgt ttatacaaac gccggtcatg atgaatgata tttcagaata tacctcgggc
     2341 taaaataccg ggacagagat caggcgtgat taatctgcag catttttaat aacagaagta
     2401 ctgagcttac tttacctttg atgatgtaat agttatttcc gtcggtactc atttttccct
     2461 gaaataatgt cacggatgtt tcttcctgct gatcctgcag gcgcagtcgt aaggtcatat
     2521 ttttaatgag ctgagatccg ggtggcaagg ggtatttgct gtcagatact gggcgactga
     2581 gtctgatgtg gcgcgtatac agcccgtttt cgtacagggt ggcctctgtt atggtgtcag
     2641 tgaccagcat gtgggagaca atgctgtatt cttttttaaa gcacccacca gaaacggtga
     2701 ttgtaatgat aatggtcttc tcgatcgaga tgtaaatacc cgtttcgtta tactgtccgt
     2761 atcggaacca tgagaactca gttgcattcc agccatgctc cagaatatgt gcattttttt
     2821 gcctgatagt ttcttttttt attcgctcat gtttaaggca taacaggcag ataaataatg
     2881 tcagaaaaag aaagctaata atgagagcaa gagcgatata gtctgtcaca gggtaataca
     2941 tattctctcc ggtagaagaa gggccgcaaa cgcggcccgg gctgttatca gaaagagtaa
     3001 cggaagttgg cgttgatcgc gttatccacg ttgtatttac caaatgccga tttctcaaac
     3061 tcaagaccga agcgaagatt atcgcgaatt tcggcgttga gaccaacatt catgagaata
     3121 cgaccgtctt tttcaccttt gatacgtttc tcaccggacg catcacgcag tacggtttca
     3181 ccgttggcaa acaggtcaaa ctggtagcca aggccggcgc gggctgtgac tttccagtcc
     3241 ttaccggaga aggatttacc cacatcaaca ccggtacgcc caatcagcgg attaaagtcc
     3301 ttatccttca tggtcacgct cattccgcga tcctgccagt cgaaccgttt acctgatacg
     3361 gccccataaa ccagttctgc ctgcggctca atccaggagt cttctgttac atgatagcgg
     3421 tagcctgctt cagcacccac atacaaggaa tgagaactgt agtcttttgt tccgagtcca
     3481 gcaaaggtcg ccgagtactc attatcatgg tgaacatact tactaatcag gtcgatatag
     3541 gcaccagagt caaatatagc ggaagcatac agacctgccc cgacggactt cgtcttgcca
     3601 ctgaatgcat tactgctggc atgactgtcg gtatacgtca tagtcagacc agtgaaaagg
     3661 tcaagtccat ccagctcatg ttttttatcc acaccaatct gcacatgggt gtagttgtca
     3721 ctgtatccac cagaagctga acctgctccg ctcatgatgc gggcccatgc accggcctcg
     3781 ccattggtgt cacgcagatc ccccattcgt ttattcaggt tgttgacttc tgtcaggaag
     3841 gctttgtaat taccggacat atacgatttt gctgcaccgg aagatgctgt attttcagcc
     3901 accttatatc cggtcagggt ccacgtggta tgttcctgat cctctttcgt gctgatcaca
     3961 ggagatatgt tgctgaaacc tatggcctga gtactggctt taaatatctc tttattgctt
     4021 ccttttgggg ctgttatcag gtcaacgttt aatcctgagt tattatttcc cactttctgt
     4081 atatcgaccg acagactgtt attgcttccg gttaactgat ttttaacaat gagtttatcc
     4141 gattgattta cagactggct cagatcagcc cgcataatca cagcagtatt agtggcatcc
     4201 agtgtgttta ctgtcacagt atggaaaccc gaattctcta tgttactaaa caacaaaaga
     4261 ctgtcgcggg tttttaatga ttgcagggct gactgtccgt ttactctcca taaggtgtta
     4321 tacatgctgg ctgaggctgc aggagcatca atacctccgg tataagaagt atcaaagcca
     4381 cccagcaaac ctgtagccag cggtgtgtgt gtggttttgt ccaggttatc ttctttatac
     4441 tggtcaacac caaacagaat agatgctttt ttgtctgatt ttatattacc attgacccag
     4501 gcataatttt gagcctttaa cgtggcacca tcttctgtca tattatatcc atctgacata
     4561 tagattgccg ggctgtattt attattttct gtaggggtgc ctgttaatgt cagggttccg
     4621 gccagttcaa tagctttatc tacatacatt tttccctggg cggtaacagt agctcctttg
     4681 ttgataatca ggtctgtatt aattaaggat aatgcaccag tcatagtaac agttgattta
     4741 tcaccaacag ttattttgct gttttgagca ctcagactgc cgttgaaact actttcgcct
     4801 gctgaatccc cggagcgaag ctgttgtctg aatccaaaac cttcgccagt aatgtttctt
     4861 cctgagaaca tgtcaatgta tgcagtttta tttcccagaa caatttgaga gtttttagcc
     4921 tctatgtgtc cttttacatt agcatcccgg cctatactca gggttgcgtt ttctaaattt
     4981 aatttccgga aattaaattt acgactttcc cagtcgggct gggaaaaaga ggctatctga
     5041 ttgttggact tatattccgt attgtaacgt ttatttacag tgctttcctg gtcctttatg
     5101 gcagcagaat agtctttttg acataaaaaa ggaattggac agttgttatt gccttcttta
     5161 aatatggcat gttctgtcgc atgtccttgt aatgttaact gtgcattccg gacagagata
     5221 tcgtttttga tatcgactga gccatcaaag atcagattgg cattattgtt atgttgctgg
     5281 gtagtattga taatatgatt tatgcttata ttacctgata cattcccatg gtacatatat
     5341 gcatcagtat tagtaagtga taatgttgat tgcttcacgt ttgagttcgt tacggttgtt
     5401 cccgcatctg ttgctgcgat tttctgaaat gactggtcat agccgtttag atccagtgtt
     5461 ccgccatttc tggtaaaata gatttcgcca ttaccgcttt cgcttaaagc gtctttggca
     5521 tttattttta ccgtaccttt acctccggcc atgtaaattt tattgaaggc tttttcagca
     5581 ctaaggatga cagtcccatt acctatttta aggttatttc cctgtgctat ttttacatcc
     5641 agagtaccac tacctatttt atgcagatta tctccggcaa cgcccttaac attccattca
     5701 acggtggtat tattatcaat atctatcccg gcacctttat aagacttatc ttttccgtac
     5761 actttatatg tctggttctt atcgaatata agcccgccaa tgccaagatc ggtatcctgc
     5821 ttgagcgtta atacccctcc tcctgagaca gacaggtcct tatcttttat aatcgatgat
     5881 aactcttgtt ttttaccatc cagctgcata gtgtttgtga aattcttttt taaattgttt
     5941 accagaatat tattaaataa cgagtagttt gtccaggcta tattggtact actgataaaa
     6001 ccaactgtac tggtaacacc atggaaaacc catttttgtt gttctttatc atatagataa
     6061 gaaccagaac cactatcgcc aaaaattggt gagtttttaa aaggggttct gttggtataa
     6121 atgtaactct tgtttttcca gtccagatta aaaagacttg cgcttaacaa gtcgggatta
     6181 tagttttgtc caaacagata ttgtttgccg tttaatatcg ttgaggttgt tccggcacct
     6241 gctctgaagc caattatttg ttccttaccg ttgtagttta cgccatatct tttcatcgca
     6301 tcttcgggag aaagtgagaa gtcggcactc tccgtaaccc ctgttgtttc cacgacgaat
     6361 ttattcagac gatgaaccga aaagtcgcca gatgatactc tgttacttgc tttatatgct
     6421 gtctgatccc atgactgtgt tgttattgca tgatgctgtg taccgttatg agtcgcagta
     6481 acactgtacg cacctccaat tgatgttgtt gcacctttgt tggaaacagc agaaaaatca
     6541 ggtacaggta gttctggaaa atgaaatatc tttccatctt tcatcattaa ttgaacattt
     6601 gttgcacccg gtctgaactc tcctctgttt tgagcaaggt caagataatc gcgggtccat
     6661 atattattta tatccagcat tgctgcaaat gctgtttcgc cataacttcc atatattgag
     6721 gataaaataa taccgaggat aactttcttt cttgatgtac cgacactaac tttccgggtc
     6781 agttcagaaa cagcaactaa tccacctgta atatgactat atttcagtga ataaatttta
     6841 ttcatacgtg aactccgata tttctgagtt gtggttatgg gatagcattt aattacaacg
     6901 gggaactatc agtcatggta cgtaactgat aagggtataa tgataatcac tgtcagttat
     6961 atttgtatga cagatgtaac aaaatgaaaa aaaagataag cacaagtgat tgattggtta
     7021 gatattgatc acttggtgtt tgtattttga attttgcgat caaaatttga tctttgttgt
     7081 ttccggtcaa tcctttacag gttttctgta ttatccgggg gagttaagtc tgtcgatgtc
     7141 cgacagactt ttgatgatgg aaacaacatg cagcgataat gtgcatgctg ttttgcaggt
     7201 ggaactcaga aggtgaactg gaactgtgcg accgcaccgt aagtgttgtc cgtgccgatc
     7261 aggcctgcaa catcaaggtg aaccacatca aacggtgaga tccccacccc ggccgtgaat
     7321 gcactgccgt cgttaccggc cagattctga cggtaaccgg cgcgcagctg tgcccatttc
     7381 caggcattaa attctgtgcc aatggcggca aactgacggt tgctgtcgga ggtgaaaccg
     7441 ctggccgggg tcagatccac atcaaatgca gtggtgaaca tcgcgttgtg ccaggagaca
     7501 ccggccgtca cctgcgggcg gatcctgaag gtttccgtga taccgttcac ttcttttgtc
     7561 tcaatactac gggagatcag gttctgtgcg accagtccca gcgtccagtt gtcatccagg
     7621 tccgtactgg caccgatgtc ggcgttgatg ccatttttcg tgttgtggta gcggtcgcct
     7681 ttgaaggcgc tgctgtcata atttctgacc agcacgttgt agttgaacag gtcgacgcgc
     7741 tggaatttgg gggggaagcc cagagaccat ttctgcccgg ccgtttccag ttccgtagcc
     7801 agagcaatac ccacatcagt gatcagtgcc gtgcgggcac aggcctgtga tgtcaggcgg
     7861 cttttgtcca cctcctgccc ggtgctgttt gccacctctt ccaggtaagt gaggtcagca
     7921 tcagagactt taccgtcgac actgacggtg ccgtgtgatt taaccatcag tgcggcagag
     7981 aggcgatcac cgggaagggc cgccacagca gaaacgccca gctgtgcggc ggcatgtgtg
     8041 ttacggaagt cccggaggtg ttctttcagg cgtgcggcag cctcaggaac accgtggtta
     8101 ctttcgatgg cgcggtcgaa ggccttccac tcatcactga tccggtcggc gttgtcggtg
     8161 atattatccg gattggagag ctgcgcgccg acggacggaa gcaggatgct gggctcatgc
     8221 gcgacgcaaa atccacgatg tatatatcag tactaaaagc gcgacggcgg aagaagctct
     8281 gaaactaatc ggtgaactgt acgccataga gcacgatata cgcgggttac cggtgtctga
     8341 acgcctggcg gtcaggcaaa tgcagagtag accgctcctg acttccctgt ataagctgat
     8401 gcaggagaaa gaacacacgt tatcgaagaa atgtcgtctg ggagatgcgt tccggtatat
     8461 caggaagcac tgggctgcgt tgtgcaactt ctgtgatgat ggcctggctg aggtagataa
     8521 taatgcagca gaaagagcgc ttcgtgcagt ctgtctcggg aagaaaactt tttcgaggat
     8581 cactcattcc gtgtctgact gcttcagggt ttaattttgc ttctgctgac tgaatgatga
     8641 ttgggcagca agtgcaatat cccacataaa tccagtcaac tcacgagcca ccgccgtaat
     8701 ggctacttgt gattttttcc cttttgctgt cagagttcgg taacgagaac acaatcgttg
     8761 ttgagctttc catgcaatat ctttggaata ttgtgttaca gagtccggtc ggtaatatat
     8821 aagccatgct ccaacttttg caggtgtacg ataagaccag gcggcttcgt aaagcagacg
     8881 tctcagttcg ctgtttccaa cttttgttat tcctctggga cgaatactgt ttccactgga
     8941 atattcacct ggcaccag
//
LOCUS       DAJTRD010000139         8771 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTRD010000139 DAJTRD010000000
VERSION     DAJTRD010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.139"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(80..871)
                     /gene="aadA1"
                     /locus_tag="OO315_003686"
     CDS             complement(80..871)
                     /gene="aadA1"
                     /locus_tag="OO315_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5892443.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(984..1814)
                     /locus_tag="OO315_003687"
     CDS             complement(984..1814)
                     /locus_tag="OO315_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5892444.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            2024..3037
                     /gene="intI1"
                     /locus_tag="OO315_003688"
     CDS             2024..3037
                     /gene="intI1"
                     /locus_tag="OO315_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5892445.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3240..3590
                     /locus_tag="OO315_003689"
     CDS             3240..3590
                     /locus_tag="OO315_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5892446.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTSEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            3716..4276
                     /locus_tag="OO315_003690"
     CDS             3716..4276
                     /locus_tag="OO315_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5892447.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            4279..7245
                     /locus_tag="OO315_003691"
     CDS             4279..7245
                     /locus_tag="OO315_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5892448.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            7312..7689
                     /locus_tag="OO315_003692"
     CDS             7312..7689
                     /locus_tag="OO315_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5892449.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7890..8549)
                     /locus_tag="OO315_003693"
     CDS             complement(7890..8549)
                     /locus_tag="OO315_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5892450.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1950 a         2479 c         2348 g         1994 t
ORIGIN      
        1 caattatgtg cttagtgcat ctaacgcttg agttaagccg cgccgcgaag cggcgtcggc
       61 ttgaacgaat tgttagacat tatttgccga ctaccttggt gatctcgcct ttcacgtagt
      121 gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttct tgtccaagat
      181 aagcctgtct agcttcaagt atgacgggct gatactgggc cggcaggcgc tccattgccc
      241 agtcggcagc gacatccttc ggcgcgattt tgccggttac tgcgctgtac caaatgcggg
      301 acaacgtaag cactacattt cgctcatcgc cagcccagtc gggcggcgag ttccatagcg
      361 ttaaggtttc atttagcgcc tcaaatagat cctgttcagg aaccggatca aagagttcct
      421 ccgccgctgg acctaccaag gcaacgctat gttctcttgc ttttgtcagc aagatagcca
      481 gatcaatgtc gatcgtggct ggctcgaaga tacctgcaag aatgtcattg cgctgccatt
      541 ctccaaattg cagttcgcgc ttagctggat aacgccacgg aatgatgtcg tcgtgcacaa
      601 caatggtgac ttctacagcg cggagaatct cgctctctcc aggggaagcc gaagtttcca
      661 aaaggtcgtt gatcaaagct cgccgcgttg tttcatcaag ccttacggtc accgtaacca
      721 gcaaatcaat atcactgtgt ggcttcaggc cgccatccac tgcggagccg tacaaatgta
      781 cggccagcaa cgtcggttcg agatggcgct cgatgacgcc aactacctct gatagttgag
      841 tcgatacttc ggcgatcacc acttccctca tgatgtttaa cgtttgaaat aaggggcgcc
      901 gagcgccagc gaggggagcc aaaagcttgc ttttggccgt cccgacttga ttgaagggtt
      961 gggcgatttt gccattagat tttttataaa tttagtgtgt ttagaatggt gatcgcattt
     1021 ttcttggctt ttatgcttga tgttaaattc gaccccaagt ttcctgtaag tgcggacaca
     1081 aaaacatatt tatgtcctga tttgcttata ataaaccctt caaaccatcc gttttgtaag
     1141 gttctatttg ctgtgaatcc tgcaccagtt ttcccataca gttttgtact attatccaga
     1201 tcttgtagat acatgttctc tatggtgttt tctatggctg agtttttaac tgggagattg
     1261 tgattaataa ttttacgcag gaattgaatt tgttcttctg gtgaaatttt taagctactt
     1321 tcgagccatg cttctgttaa tccgttgttt ctttctttat ctccagagaa gtcttgattt
     1381 ccataatcaa aatctttgag ataattcttg attttattta atccaatttt ttgggttatt
     1441 tcttgcgaaa cccaaacaac agaaaattgc atccacgtct ttggtgtatg attgctgttc
     1501 cagatctcca ttcctttggg ggttttatcc catttgaata tggttttctg atctattatt
     1561 tccgcatcaa atgccataag tgataatgcg atcttgaaag ttgaatctgg tgccatttgc
     1621 gttgcacact ttgctttatt gaattgagca atttcagcgt ttgtggatgc atcgtaaagt
     1681 aaaaaacaac cttcagttcc ttcaaataat ggagatgcaa cagtagagat atctgttgat
     1741 gcactggcgc tgctgtagat aatatttgca attattaaaa aaatagcgaa gttgatatgt
     1801 attgtgtttt tcataataag tattggtttg gtaaagggct taattttaac ggctaacaat
     1861 taatgaggct ccgggttcgc ccaactttgt tttagggcga ctgccctgct gcgtaacatc
     1921 gttgctgctc cataacatca aacatcgacc cacggcgtaa cgcgcttgct gcttggatgc
     1981 ccgaggcata gactgtaccc caaaaaaaca gtcataacaa gccatgaaaa ccgccactgc
     2041 gccgttacca ccgctgcgtt cggtcaaggt tctggaccag ttgcgtgagc gcatacgcta
     2101 cttgcattac agcttacgaa ccgaacaggc ttatgtccac tgggttcgtg ccttcatccg
     2161 tttccacggt gtgcgtcacc cggcaacctt gggcagcagc gaagtcgagg catttctgtc
     2221 ctggctggcg aacgagcgca aggtttcggt ctccacgcat cgtcaggcat tggcggcctt
     2281 gctgttcttc tacggcaagg tgctgtgcac ggatctgccc tggcttcagg agatcggaag
     2341 acctcggccg tcgcggcgct tgccggtggt gctgaccccg gatgaagtgg ttcgcatcct
     2401 cggttttctg gaaggcgagc atcgtttgtt cgcccagctt ctgtatggaa cgggcatgcg
     2461 gatcagtgag ggtttgcaac tgcgggtcaa ggatctggat ttcgatcacg gcacgatcat
     2521 cgtgcgggag ggcaagggct ccaaggatcg ggccttgatg ttacccgaga gcttggcacc
     2581 cagcctgcgc gagcagctgt cgcgtgcacg ggcatggtgg ctgaaggacc aggccgaggg
     2641 ccgcagcggc gttgcgcttc ccgacgccct tgagcggaag tatccgcgcg ccgggcattc
     2701 ctggccgtgg ttctgggttt ttgcgcagca cacgcattcg accgatccac ggagcggtgt
     2761 cgtgcgtcgc catcacatgt atgaccagac ctttcagcgc gccttcaaac gtgccgtaga
     2821 acaagcaggc atcacgaagc ccgccacacc gcacaccctc cgccactcgt tcgcgacggc
     2881 cttgctccgc agcggttacg acattcgaac cgtgcaggat ctgctcggcc attccgacgt
     2941 ctctacgacg atgatttaca cgcatgtgct gaaagttggc ggtgccggag tgcgctcacc
     3001 gcttgatgcg ctgccgcccc tcactagtga gaggtagggc agcgcaagtc aatcctggcg
     3061 gattcactac ccctgcgcga aggccatcgg tgccgcatcg aacggccggt tgcggaaagt
     3121 cctccctgcg tccgctgatg gccggcagca gcccgtcgtt gcctgatgga tccaacccct
     3181 ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag ccgtcttctg aaaacgacaa
     3241 tggaggtggt agccgagggt gtggaaacac ccgactgcct tgcgtggttg cggcaggcgg
     3301 gttgcgacac ggtgcagggt ttcctgttcg ccaggccgat gccggcggcg gccttcgtcg
     3361 gcttcgtcaa ccaatggagg aacaccacca tgaacgccaa tgaaccgagc accagttgct
     3421 gcgtgtgctg caaggaaatc ccgctcgatg ccgccttcac gtcggaaggg gccgagtacg
     3481 tggagcattt ctgcgggctg gagtgctatc agcgcttcca ggcgcgggcc agcactgcga
     3541 ccgaaaccag cgtcaaaccg gacgcttgtg attcgccgcc gtcaggttga ggcataccct
     3601 aacctgatgt cagatgccat gtgtaaattg cgtcaggata ggattgaatt ttgaatttat
     3661 tgacatatct cgttgaaggt catagagtct tccctgacat tttgcaggga attccatgac
     3721 tggacagcgc attgggtata tcagggtcag caccttcgac cagaacccgg aacggcaact
     3781 ggaaggcgtc aaggttgatc gcgcttttag cgacaaggca tccggcaagg atgtcaagcg
     3841 tccgcaactg gaagcgctga taagcttcgc ccgcaccggc gacaccgtgg tggtgcatag
     3901 catggatcgc ctggcgcgca atctcgatga tttgcgccgg atcgtgcaaa cgctgacaca
     3961 acgcggcgtg catatcgaat tcgtcaagga acacctcagt tttactggcg aagactctcc
     4021 gatggcgaac ctgatgctct cggtgatggg cgcgttcgcc gagttcgagc gcgccctgat
     4081 ccgcgagcgt cagcgcgagg gtattgcgct cgccaagcaa cgcggggctt accgtggcag
     4141 gaagaaatcc ctgtcgtctg agcgtattgc cgaactgcgc caacgtgtcg aggctggcga
     4201 gcaaaagacc aagcttgctc gtgaattcgg aatcagtcgc gaaaccctgt atcaatactt
     4261 gagaacggat cagtaaatat gccacgtcgt tccatcctgt ccgccgccga gcgggaaagc
     4321 ctgctggcgt tgccggactc caaggacgac ctgatccgac attacacatt caacgatacc
     4381 gacctctcga tcatccgaca gcggcgcggg ccagccaatc ggctgggctt cgcggtgcag
     4441 ctctgttacc tgcgctttcc cggcgtcatc ctgggcgtcg atgaactacc gttcccgccc
     4501 ttgttgaagc tggtcgccga ccagctcaag gtcggcgtcg aaagctggaa cgagtacggc
     4561 cagcgggagc agacccggcg cgagcacctg agcgagctgc aaaccgtgtt cggtttccgg
     4621 cccttcacca tgagccatta ccggcaggcc gtccagatgc tgaccgagct ggcgatgcaa
     4681 accgacaaag gcatcgtgct ggccagcgcc ttgatcgggc acctgcggcg gcagtcggtc
     4741 attctgcccg ccctcaacgc cgtcgagcgg gcgagtgccg aggcgatcac ccgtgctaac
     4801 cggcgcatct acgacgcctt ggccgaacca ctggcggacg cgcatcgccg ccgcctcgac
     4861 gatctgctca agcgccggga caacggcaag acgacctggt tggcttggtt gcgccagtct
     4921 ccggccaagc caaattcgcg gcatatgctg gaacacatcg aacgcctcaa ggcatggcag
     4981 gcactcgatc tgcctaccgg catcgagcgg ctggttcacc agaaccgcct gctcaagatt
     5041 gcccgcgagg gcggccagat gacacccgcc gacctggcca aattcgagcc gcaacggcgc
     5101 tacgccactc tcgtggcgct ggccaccgag ggcatggcca ccgtcaccga cgaaatcatc
     5161 gacctgcacg accgcatcct gggtaagctg tttaacgctg ccaagaataa gcatcagcag
     5221 cagttccagg cgtcaggcaa ggccatcaac gccaaggtac gtctgtacgg gcgcatcggt
     5281 caggcgctga tcgacgccaa gcaatcaggc cgcgatgcgt ttgccgccat cgaggccgtc
     5341 atgtcctggg attcctttgc cgagagcgtc accgaggcgc agaagctcgc gcaacccgat
     5401 gacttcgatt tcctgcatcg catcggcgag agctacgcca ccctgcgccg ctatgcaccg
     5461 gaattccttg ccgtgctcaa gctgcgggcc gcgcccgccg ccaaaaacgt gcttgatgcc
     5521 attgaggtgc tgcgcggcat gaacaccgac aacgcccgca agctgccagc cgatgcaccg
     5581 accggcttca tcaagccgcg ctggcagaaa ctggtgatga ccgacgccgg catcgaccgg
     5641 cgctactacg aactgtgcgc gctgtccgag ttgaagaact ccctgcgctc gggcgacatc
     5701 tgggtgcagg gttcacgcca gttcaaggac ttcgaggact acctggtacc gcccgagaag
     5761 ttcaccagcc tcaagcagtc cagcgaattg ccgctggccg tggccaccga ctgcgaacaa
     5821 tatctgcatg agcggctgac gctgctggaa gcacaacttg ccaccgtcaa ccgcatggcg
     5881 gcagccaacg acctgccgga tgccatcatc accgagtcgg gcttgaagat cacgccgctg
     5941 gatgcggcgg tgcccgacac cgcgcaggcg ctgatagacc agacagccat ggtcctgccg
     6001 cacgtcaaga tcaccgaact gctgctcgaa gtcgatgagt ggacgggctt cacccggcac
     6061 ttcacgcact tgaaatcggg cgatctggcc aaggacaaga acctgttgtt gaccacgatc
     6121 ctggccgacg cgatcaacct gggcctgacc aagatggccg agtcctgccc cggcacgacc
     6181 tacgcgaagc tcgcttggct gcaagcctgg catacccgcg acgaaacgta ctcgacagcg
     6241 ttggctgaac tggtcaacgc tcagtttcgg catccctttg ccgggcactg gggcgatggc
     6301 accacatcat catcggacgg acagaatttc cgaaccgcta gcaaggcaaa gagcacgggg
     6361 cacatcaacc caaaatatgg cagcagccca ggacggactt tctacaccca catctccgac
     6421 caatacgcgc cattccacac caaggtggtc aatgtcggcc tgcgcgactc aacctacgtg
     6481 ctcgacggcc tgctgtacca cgaatccgac ctgcggatcg aggagcacta caccgacacg
     6541 gcgggcttca ccgatcacgt cttcgccctg atgcacctct tgggcttccg cttcgcgccg
     6601 cgcatccgcg acctgggcga caccaagctc tacatcccga agggcgatgc cgcctatgac
     6661 gcgctcaagc cgatgatcgg cggcacgctc aacatcaagc acgtccgcgc ccattgggac
     6721 gaaatcctgc ggctggccac ctcgatcaag cagggcacgg tgacggcctc gctgatgctc
     6781 aggaaactcg gcagctaccc gcgccagaac ggcttggccg tcgcgctgcg cgagttgggc
     6841 cgcatcgagc gcacgctgtt catcctcgac tggctgcaaa gcgtcgagct acgccgccgc
     6901 gtgcatgccg ggctgaacaa gggcgaggcg cgcaatgcgc tggcccgtgc cgtgttcttc
     6961 aaccgccttg gtgaaatccg tgaccgcagt ttcgagcagc agcgctaccg ggccagcggc
     7021 ctcaacctgg tgacggcggc catcgtgctg tggaacacgg tctacctgga gcgtgcggcg
     7081 catgcgttgc gcggcaatgg tcatgccgtc gatgactcgc tattgcagta cctgtcgcca
     7141 ctcggctggg agcacatcaa cctgaccggt gattacctat ggcgcagcag cgccaagatc
     7201 ggcgcgggga agttcaggcc gctacggcct ctgcaaccgg cttagcgtgc tttattttcc
     7261 gttttctgag acgaccccta ttatggcgaa ggtatcattc aggaagcgtt gatgaaggat
     7321 tatctctgta aaaagttatt caaccggctt tccggtaccc tggtgatcag agcgcgctgc
     7381 ggcaataaca ttactggcct ggcatgttgc aatattcttt atccctcgcc ccgatacagc
     7441 ggtcagctgc atattaaaga gctgtatgtt tctcagtgcg atcgaaataa gggcacgggt
     7501 aaagcgataa tgcgctttat agcccggctt gcgcttgaac aggaatgcct tagccttagc
     7561 tggaacgctg aaaaatccaa ccccggcgct aaccgttttt atcaggctct gggaggcaga
     7621 ataaatgatc atatcgtcaa ttattacctc cacggggaga gcctgagcaa actggcctca
     7681 ggcatttgag aagcacacgg tcacactgct tccggtagtc aataaaccgg taaaccagca
     7741 atagacataa gcggctattt aacgaccctg ccctgaaccg acgaccgggt cgaatttgct
     7801 ttcgaatttc tgccattcat ccgcttatta tcacttattc aggcgtagca accaggcgtt
     7861 taagggcacc aataactgcc ttaaaaaaat tacgccccgc cctgccactc atcgcagtac
     7921 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     7981 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     8041 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     8101 cagggattgg ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     8161 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     8221 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     8281 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg gaattccgga
     8341 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     8401 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     8461 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     8521 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     8581 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     8641 acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc ggtatcaaca
     8701 gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat ttattcggcg
     8761 caaagtgcgt c
//
LOCUS       DAJTRD010000140         8655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTRD010000140 DAJTRD010000000
VERSION     DAJTRD010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.140"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(789..1985)
                     /gene="hemY"
                     /locus_tag="OO315_003694"
     CDS             complement(789..1985)
                     /gene="hemY"
                     /locus_tag="OO315_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5892451.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1988..3193)
                     /gene="hemX"
                     /locus_tag="OO315_003695"
     CDS             complement(1988..3193)
                     /gene="hemX"
                     /locus_tag="OO315_003695"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5892452.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            complement(3215..3955)
                     /gene="hemD"
                     /locus_tag="OO315_003696"
     CDS             complement(3215..3955)
                     /gene="hemD"
                     /locus_tag="OO315_003696"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5892453.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWYFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3952..4893)
                     /gene="hemC"
                     /locus_tag="OO315_003697"
     CDS             complement(3952..4893)
                     /gene="hemC"
                     /locus_tag="OO315_003697"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5892454.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5280..7826
                     /gene="cyaA"
                     /locus_tag="OO315_003698"
     CDS             5280..7826
                     /gene="cyaA"
                     /locus_tag="OO315_003698"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5892455.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7866..8186)
                     /gene="cyaY"
                     /locus_tag="OO315_003699"
     CDS             complement(7866..8186)
                     /gene="cyaY"
                     /locus_tag="OO315_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5892456.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8234..>8655)
                     /locus_tag="OO315_003700"
     CDS             complement(8234..>8655)
                     /locus_tag="OO315_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5892457.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1971 a         2238 c         2226 g         2220 t
ORIGIN      
        1 attcaggggg ccagtctagc agttgacggg gtatctgctg cgctggccat gagaggaaga
       61 gcggcggcga cagcaacaac aagacgtttg ggtgaaaacg aaaattccat gcaaaatgct
      121 ccggtttcat gtcatcaaaa tgatgacgta attaagcatt gataattgag atccctctcc
      181 ctgacaggat gattacataa ataatagtga caaaaataaa ttatttattt atccagaaaa
      241 tgaattggaa aatcaggaga gcgttttcaa tcctacctct ggcgcagttg atatgtaagg
      301 caggtttatt atatcgcgtt gattattgat gctgttttta gttttaacgg caattaatat
      361 atgtgttatt aattgaatga attttatcat tcataataag tatgtgtagg atcaagctca
      421 ggttaaatat tcactcagga agttattact caggaagcaa agaggattac agaattatct
      481 cataacaagt gttaagggat gttatttccc gattctctgt ggcataataa acgagtagat
      541 gctcattcca tctcttatgt tcgccttagt gcctcataaa ctccggaatg acgcagagcc
      601 gtttacggtg cttatcgtcc actgacagat gtcgcttatg cctcatcaga caccatggac
      661 acaacgttga gtgaagcacc cacttgttgt catacagacc tgttttaacg cctgctccgt
      721 aataagagca ggcgtttttt tatgcatcag aaaggtcccg gaggtgcttt cctccgggtg
      781 agaaggaact actgtggcgg gttattctgc aacgttaaca tcaaaccgtc gcgacgcatc
      841 gctgcagctt cttccggctt gtgcagtctg tccagcgcgt cggcaagcca tgcgtaatcg
      901 taggcgtccg gacgttgttt cagcgccgcg cggaaggcga gcgatgcttc ctgccattca
      961 ccgtgcttca tcagtgactg acccaatgtg ctccacaaca gcgggcgatc accgacgttt
     1021 ttgatttgct ggcgtagcac tttttccagc tgttccgggt tattggtttt cagacgcggg
     1081 atcggcagca gtaggcgatc gtcatactgg cgtttcaggc cgtcgatgat aatttgctgg
     1141 gcagtatcat gatcgtcaca ttcaataaga tgttccgcca ttgccacctg caacgctacc
     1201 tgatgacgcg ttttccggct ttggtttttc caccagttac gcaaaccttc actaccgtta
     1261 tcggcacgcg cctgatccat caggccaatc catgcctgtt gttccagcat tgcacgatgt
     1321 tcttcatcac caacatgggc tttcgccatt gatgggatga tatccagcag agaactccat
     1381 gcccctgtgc ggatatacgc ctgttccgcc agacgtaata cttcaggatg gcgtggcgta
     1441 acttccagca gcttatccac gccgtggcgt gcagcatggt tttcattacg ggccagttgc
     1501 agacgtacac gggtgatttc taccggaatg gtgtcattgc cggccagctc cgctgcgcgt
     1561 tccagatgtt ggttggcgcg tgcttcatca ccacgttgtt gcgcggcttc ggcagccagt
     1621 agatagttca ccaccggttg ttcagcgtga tcggcatttt tcgccatcag cttttcaact
     1681 tgctgataat caccttccgc cagtttcagc agcgcctgtt cggtctgctt acgcgcacgg
     1741 cgacgcttac gtccgacaaa ccatccacgg gtgtgcgcgc cagtgcggaa gatccgccgc
     1801 agtagccact caatggcaaa cagcactacc atcgccagaa tcaatatgat cgccaggccc
     1861 gtgacgctgg tttcgatatt gtagttgtcg gtctggatca gcacataacc ctgatgaccg
     1921 gcaatcatcg ggccaaccac tatccccgca atcagcaaaa caaagagtaa taacactttt
     1981 agcatggtta ttctccttgc ggcgcggctg ccggagtatc accttgcggt gcaggtgcag
     2041 gtgcaggtgc aggtgcaggt gcaggtttag cttccgttgt ccccgctgcc ggttgtgcca
     2101 gcaggttacg cacgcgagtc tgcatcagtt tttccagcat cgcctggctt tgcagggttt
     2161 ccggaagatc catcgagata ttttgctggc ttaactggtc cacctcgtcg aggaacgctt
     2221 tggtggtggc atcatcagta tcgtagtaag cacgtaccca ggtggagacg ttctccagcg
     2281 cctggcgata agtctcttcc tgatgacgcg gtacagcttg tgctgcgacc agcaggcgag
     2341 agcgaatatt ttcgcgcaga tagatatcct gatttggcgc taacagcggt acggcggtgt
     2401 catcacgacg gcgaatcgta atgaagttgt ccataaagtt ctgccagctt ttttgcagat
     2461 tgatacgcca ttcgctgatg gaactggaaa gctcttcacc gtctgaatcc atcggcgaac
     2521 catcgctgtc attatcggcc agacgcaggt tatctacctg atttgaaagc tgattaagct
     2581 taaggatgat gccgtcataa tccacctgcg atactgcaga aaggctggcg atatcatcgg
     2641 taattgcccg acgaacggta atcagactcg ggtcattcat atccgccagg ctggcgtctg
     2701 cacttttcag caacgctgca gaggtcgtga cgtcctgatc gctccacagc ttccgtccgg
     2761 cgagtttcac cagaaaatcg gcctgagcca gcagccaggt tttagcatcg ctgccggaaa
     2821 tggtggcgat cttttgttgg acttcatcca actgttttgc cagcgtttct tgctgacgat
     2881 tcgcctgctt aagctgcgca gcttgttgct taataatgcc ttccagctcg gctttttggc
     2941 tctcctgggc tttttgcaat gccgtcagtt ggttagccag ggcatcgctg gtggcggtct
     3001 gattgacggc ctgttgttta ccccagccat acaaaccgac acccgccgcc agagcaatag
     3061 cgatagccac cgcgctgaga atcaatgcag tattgttctt actctttttt tctgttgcga
     3121 caggttgtga cgtggtgtcc acggcctccc tggtctcttc aaccacggcg gaggtttttt
     3181 cttgttccgt cattatggct tcctgttatg agagttattg taatgcccgt aaaagcgcat
     3241 cgttgtcagc gttatcggcg accttaatgt cttgccagcc cagttcccgg gcgagtttcg
     3301 ccaaacgctc actgacgacc aatagtcgac agtgtagtaa ccagtgctca cgataccatt
     3361 gtgggatcag cgaccagagt tgctgcaaca tttcaccgct ggtaacaacg accatcgtca
     3421 cctcgcggga ttgccagcgc atcgcttctt ctgcaccatc gtaatggatt gcgcatcgtt
     3481 gataacattc acaaaaagtg acctcagcac cgcgcaccgt cagggtatcc ccaattagct
     3541 cacgaccgcc attgccacgt aatatcagcg cacgtttgcc cgcaatattt tgtaattcag
     3601 gtaattgtag caagacttcg ctgatttccc gatcctgtgg gtagagaatc ttctgtccgc
     3661 ttacggtatg tagcgccagt gcggtggtgc gtccaatggc gaaataagta ggtagttggg
     3721 gccatttacg atcttgctga tgcagctgtg attgggcaaa agcaaccgcg tgttgcgaga
     3781 gggcaaacaa cagatcgctc tcccccagcg ctgccagttg atcagcaagt tgcggtaatt
     3841 gtcgacccgg agaaaactca atcagcggaa aataccaggc cacctgcccc agtgtgcgca
     3901 gacggctcac taactcttct ccagcgggag acgggcgggt gaccaggata ctcatgccgg
     3961 ggcctctccg ttatagactt cagcgaggat ctcgcgcgcg ccgttattca gtagctcttc
     4021 tgccagcgaa atccccattt gttcggcatc ttgcggcgca ccgcggcgtt caccgcgaat
     4081 aatctgcgaa ccgtccggcg cgccgaccag cgcacatagc cagatttcgc catcaataag
     4141 ctcggcgtag ctaccaattg gcacctgaca tccgccttcg agacgggtat tcatggcgcg
     4201 ttctgcggta acgcgcagtg cagtttcgtg gtgattcagc gcggcaagca gctcgcgagt
     4261 gcgtgtatca tcaaggcggc attcaatacc gactgcacct tgtcctaccg ccggaagaga
     4321 aatctcgggt ggtaacgcgg cgcgaatgcg ggactccaga cctaaacgtt ttagtccggc
     4381 tacggcaaga atgatggcat cgtattcgcc gttatctagt ttgctcaggc gagtgccgac
     4441 gttgccgcgc agggagcgga taatcagatc cgggcggcgt tcagccagtt ggcactggcg
     4501 acgtaaactg gacgtcccga cgatactgcc tgctggtaac gcatccagac tgtcatagtt
     4561 attggacaca aaggcatcgc gaggatcttc acgctcgcaa atagtgacca gccccagacc
     4621 ttgcgggaat tcaaccggca catctttcat tgaatgtacg gcgatatcag cgcgattctc
     4681 caggagcgcg acttccagct cttttacaaa taagcctttt ccgcctactt tcgccagcgg
     4741 cgtatcaaga atcacatcgc cgcgcgtcac catcggtacc agttcaacga ccaggcccgg
     4801 atggctcgcc atcaacttgt ctttgacata gtgtgcctgc cagagtgcaa gtgggctttg
     4861 gcgtgtggca attcttaaaa cattgtctaa catgcttgtt accgtcatta tcatccgtgg
     4921 tccatcctaa catccttgcc agagtgatgt cagtgttgtg gtgaaacgta gacgcctgcg
     4981 caaaccgtaa aatgaggtct ggcagtggat cctgacaggc gtttcacgcc gtcgtaataa
     5041 ggaatttaca gagaataaac ggtgctacac ttgtatgtag cgcatctttc tttacggtca
     5101 atcagcaagg tgttaaattg atcacgtttt agaccatttt ttcgtcgtga aactaaaaaa
     5161 accaggcgcg aaaagtggta acggttacct ttgacatacg aaatatcccg aatgccgcgt
     5221 gttaccgttg atgttggcgg aatcacagtc atgacgggta gcaaatcagg cgatacgtct
     5281 tgtacctcta tattgagact ctgaaacaga gactggatgc cataaatcaa ttgcgtgtgg
     5341 atcgcgcgct tgctgctatg gggcctgcat tccaacaggt ctacagtcta ctgccgacat
     5401 tgttgcacta tcaccatccg cgaatgccgg gttaccttga tggtaacgtt cccaaaggca
     5461 tttgccttta cacgcctgat gaaactcaac gccactacct gaacgagctt gaactgtatc
     5521 gtggaatgtc agtacaggat ccaccgaaag gtgagcttcc aattactggt gtatacacca
     5581 tgggcagcac ctcgtccgta gggcaaagtt gttcctctga cctggatatc tgggtctgtc
     5641 atcaatcctg gctcgatagc gaagagcgcc agttactaca acgtaaatgc agcctgctgg
     5701 aaagctgggc cgcctcgctg ggtgtggaag tcagcttctt cctgattgat gaaaaccgct
     5761 tccgtcataa tgaaagcggc agcctggggg gcgaagattg tggctccacc cagcatatac
     5821 tgctgcttga cgaattttat cgtactgccg tgcgtctcgc cggtaagcgt attctgtgga
     5881 atatggtgcc gtgcgacgaa gaagagcatt acgacgacta tgtgatgacg ctttacgcgc
     5941 agggcgtgct gacgccaaat gaatggctgg atctcggtgg cttaagctcg ctttctgctg
     6001 aagagtactt tggtgccagc ctttggcagc tctacaagag tatcgattcc ccatacaaag
     6061 cggtgctgaa aacactgctg ctggaagcct attcctggga atacccgaac ccacgtctgc
     6121 tggcgaaaga tatcaaacag cgtttgcacg acggcgagat tgtatcgttt ggcctcgatc
     6181 catactgcat gatgctggag cgtgttactg aatacctgac ggcgattgaa gatttcaccc
     6241 gtctggattt agtacgtcgc tgcttctatt taaaagtgtg cgaaaaactc agccgtgaac
     6301 gcgcctgtgt aggctggcgt cgcgcagtgt tgagccagtt agtgagcgag tggggttggg
     6361 acgaagctcg tctggcaatg ctcgataacc gtgctaactg gaagattgat caggtgcgtg
     6421 aggcgcacaa cgagctgctc gacgcgatga tgcagagcta ccgtaatctg atccgctttg
     6481 cgcgtcgcaa taaccttagc gtctccgcca gtccgcagga tatcggcgta ctgacgcgta
     6541 aactgtatgc cgcgtttgaa gcattgccag gtaaagtgac gctggtaaac ccgcagattt
     6601 cacccgatct ctcggaaccg aatctgacct ttatttatgt gccgccgggc cgggctaacc
     6661 gttcaggttg gtatctgtat aaccgcgcgc caaatattga gtcgatcatc agccatcagc
     6721 cgctggaata taaccgttac ctgaataaac tggtggcgtg ggcatggttt aacggcctgc
     6781 tgacctcgcg cacccgtttg tatattaaag gtaacggcat tgtcgatttg cctaagttgc
     6841 aggagatggt cgccgacgtg tcgcaccatt tcccgctgcg cttacctgca ccgacaccga
     6901 aggcgctcta cagcccgtgt gagattcgcc atctggcgat tatcgttaac ctggaatatg
     6961 acccgacagc tgcgttccgc aatcaggtgg tgcattttga tttccgtaag ctggatgtct
     7021 tcagctttgg cgagaatcaa aattgcctgg taggtagcgt tgacctgctg taccgcaact
     7081 cgtggaacga agtgcgtacg ctgcacttca acggcgagca atcgatgatc gaagccctga
     7141 aaactattct cggcaaaatg catcaggacg ccgcaccgcc agatagcgtg gaagtcttct
     7201 gttatagcca gcatctgcgc ggcttaatcc gtactcgcgt gcagcaactg gtttctgagt
     7261 gtattgaact gcgcctttcc agcacccgcc aggaaaccgg gcgtttcaag gcgctgcgcg
     7321 tttctggtca aacctggggc ttgttcttcg aacgcctgaa tgtatcggta cagaagctgg
     7381 agaacgccat cgagttttat ggcgctattt cgcataacaa actgcacggc ttgtcagtgc
     7441 aggttgaaac caatcacgtc aaattaccgg cggtggtgga cggctttgcc agcgaaggga
     7501 tcatccagtt ctttttcgaa gaaacgcaag acgagaatgg ctttaatatc tacattctcg
     7561 acgaaagcaa ccgggttgag gtgtatcacc actgcgaagg cagcaaagag gagctggtac
     7621 gtgacgtcag tcgcttctac tcgtcatcgc acgaccgctt cacctacggc tcaagcttca
     7681 tcaacttcaa cctgccgcag ttctatcaga ttgtgaaggt tgatggtcgc gaacaggtga
     7741 ttccgttccg aacaaaatcg attggcaacc tgccgcccgc caatcaggat cacgatacgc
     7801 cgctattaca gcagtatttt tcgtgatgag cgtgccggaa agcgaggctt atccggcatg
     7861 caatcttagc ggaaactgac tgtttcaccc gcctgttgcg tcgccgcctg ttccagcaaa
     7921 tcccagaagg tttcgccgct gcgatcgcaa atccattcat cgcctttcag gtcaaaatgg
     7981 tagccgccct gtttggttgc cagccacacc tggtgcagcg gctcctggcg gttaataatg
     8041 attttgctgc cgttttcaaa ggtaatggtc agtacgccgc cgttgatttc gcagtcgata
     8101 tcgctgtcgc catccctgtc gtccagacgc tcttcaatgg tcagccacag ttgatcagcc
     8161 aggcgatgaa attcactgtc gttcattgtt gtatcctgtt tttaagtgat ggcggcagta
     8221 tagcggcatg gggtcagggc ttcaaagttt gcacctctgc tgctgcgttc cggcatgatt
     8281 catccgtcac cggaataatg atgtctctgt tttgaacttc acgtacgttc tcctctctga
     8341 cacgagtttt cgccgggcag ggggagggcg taatccttac ctctgtatcg gtccatgtat
     8401 aaacattgcg atcgtcataa gcgaagcgat cgtttttagt tagcgaaccg ttaactggaa
     8461 taatttgcaa gctctttgac cgtaattggc ggataaagta agcatgctgt ttgccgctat
     8521 taaatgagcg atcaatgacc aggtctggcg tatccagcgg gattatctca agctggtctt
     8581 caccaaatgt cgataatttt gttacataca gccgatcttt atattgcgca atattgccag
     8641 taatcagatg gaatt
//
LOCUS       DAJTRD010000141         8532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTRD010000141 DAJTRD010000000
VERSION     DAJTRD010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.141"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(1..86)
                     /locus_tag="OO315_003701"
                     /pseudo
     CDS             complement(1..86)
                     /locus_tag="OO315_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            84..408
                     /locus_tag="OO315_003702"
                     /pseudo
     CDS             84..408
                     /locus_tag="OO315_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(438..3350)
                     /locus_tag="OO315_003703"
     CDS             complement(438..3350)
                     /locus_tag="OO315_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892458.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3353..3469
                     /locus_tag="OO315_003704"
     CDS             3353..3469
                     /locus_tag="OO315_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5892459.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3833..4675
                     /locus_tag="OO315_003705"
     CDS             3833..4675
                     /locus_tag="OO315_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5892460.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4678..5766
                     /gene="capU"
                     /locus_tag="OO315_003706"
     CDS             4678..5766
                     /gene="capU"
                     /locus_tag="OO315_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5892461.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5771..6721
                     /gene="virK"
                     /locus_tag="OO315_003707"
     CDS             5771..6721
                     /gene="virK"
                     /locus_tag="OO315_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5892462.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6786..7730
                     /gene="lpxM"
                     /locus_tag="OO315_003708"
                     /gene_synonym="msbB"
     CDS             6786..7730
                     /gene="lpxM"
                     /locus_tag="OO315_003708"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5892463.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(7884..>8532)
                     /locus_tag="OO315_003709"
     CDS             complement(7884..>8532)
                     /locus_tag="OO315_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5892464.1"
                     /translation="AGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEKMAAIHKALDECS
                     AEHPVFYEDEVDIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVG
                     GNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPV
                     YSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT         2249 a         1949 c         2024 g         2310 t
ORIGIN      
        1 tcctgctccg acatacctgc aggcaaaggc catgaaagcc ctgatttccg gaagcgagcg
       61 aacagtccac aaactgttga acgtggtgtc agcggcggac agggataagc cctcgtcagc
      121 agttccgtca gcactgcgac agcgttcttt acccggtcaa aacagcgttg cggtgcacca
      181 cgcctgacgg atgaactgcg tgctcagggt taccacttta acgtaaaaac cgtggcggca
      241 agcctgcgcc gtcaggggct gagggcaaag gcctcccgga agttcagccc ggtcagttac
      301 cgcgcacatg gcctgcccgt gtcagaaaat ctgctggagc aggattttta cgccagtggt
      361 ccgaaccaga agtgggcggg agacatcacg tacttacgta cagatgaagt tcggttacat
      421 cctgtcagca cggaacctca cgctttttga gcggcacgac ggatcccccc cacccagtat
      481 ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat atccactaat
      541 tccggtgaca tatcctgcag ataagtctgc aaatcatgcc ccagatcttc cggtgactgc
      601 aactcataaa ccgcgcgcca cacatcaccc ttccttattt taagccccgg gttaagaata
      661 tactgtccgt ggttcagcct cagaaaaagc ttttttccat aaggattttt gctgttaact
      721 tcatgttgag agagctggct gctgatataa ctccttcgtt tgcggcgttc cggcagcaac
      781 gcttcaggaa gatgaaccac acactctgcc agtcgtgccg cactgaaggc ttccgaaaaa
      841 aacagagcac ttcccagatg cgtgtggaac agcgcctgca tcaggatcac cagaaacatt
      901 gccatctgcc ggttgtccag tttgatgagg cgtccttcca cctgcagtga cagactttca
      961 ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg cataacgggg cgtactgacc
     1021 gccgctgaca gaacttgatg ataagccgcc agcccgtcat taccggtcag caagggatct
     1081 gcaccacgct ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc tgctgacatc
     1141 agcggagtaa gattaaacgg cgaacggtga tcaacaccat aacgttcaat atcccgcaac
     1201 acggcaaccg ggttattcat tgagtatggc cggaaatact tcagctccag taactgcaga
     1261 gtcttcgttc tgggctgcct tgtgggctta aaatcttccc ggtacagtgc attatacagc
     1321 gggaacaggg aataaatcag actgtactcc agcgcctgca gttgtatggt tttatcggct
     1381 gtacctgcga ggatatgctg tttccactgt cgtgcatcct ctgcggtgat aatctgccat
     1441 ggcatctcag atgtctgcag gatccggcta cggatggtgt ccgcctgctc ctgcttcccc
     1501 tgtttttcaa gcagatgtgc ggttttctgc cactcatcac ggctggaaat ctcttcctgc
     1561 agattcagag gctcttcctg atgcgtgagc gcaagcagac tccacagagg atgctccacc
     1621 tgctgttcaa caagatacac attgtgttta gcacgggtga gcgcaacata cagcgcgttg
     1681 gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt cccgcggacg ggaataacgg
     1741 gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa tgcctgccgg
     1801 gcagcgctta ctatattgta aaggatcacc gtttcgtatt ccagcccctt aacctcctga
     1861 accgaaaaga ccagcggcgt actgaaccag catcgcgcct gtgctttctg ttccggatgc
     1921 atcacaatga ccgctacacg attcgaaagt gaggttttgg cattcagttc ctgctttgtc
     1981 tcttccctat cgtccagcaa ccggatggtc ccttctgcct gaccgcagct tctgacaaaa
     2041 tggtgacttt cccgatcaat agcactaaag cggacctgtt tgagtctcag caaccggtta
     2101 gccagtgcag tgacgtggtg cccgttacgg taattcgcct gcaggatatt aacggttgtg
     2161 tcgtttccct gttgctgccg gaaaaagaga cttttgagcg aactccatga gaaaaagctc
     2221 ggatgaacta tctgattggc atcaccacag agtaaaaact gaccgggatg acgtagcgtt
     2281 ttcagtacca gttgcagttg aacaggcgtc atatcctgaa cttcatcaac aaaaatgaca
     2341 tcataacggg gagttgccaa cgaaagatac tgatgactga gcagattact gtcatactga
     2401 tgcgactgtt tcagccatgc gatataacgt tcaaacagga catagaccgt cgggcggtcc
     2461 tccatgccat agagcgactg ccgtatcccc agtgacagat acgcctcccg ggacagcggc
     2521 ccattcccgg aggcaaccgc accgatcacg cccctgaact cctcatacag ggtatgggca
     2581 gcccccagtg cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc actgaaagtc
     2641 acctccctgc cttccggaat gcgcagagtt tccagaaatt ccgtcagcga aagaaagtca
     2701 atattctgta cctcactccc ttcccctgaa gcgtcataga gagtacgcgc tttttccacc
     2761 agaaatgagg aaaggctgag atacagtata tcaccagcag cctgcttcat tttttccagt
     2821 accagcgccg tcttaccact tcccgccgag ccaacaatca ccagcggtag cgggtgttcg
     2881 tacagtgcct gctgatcatc atcaaaggac agcattttat ccaggcggtg gaaacgccca
     2941 tgagacggat tgatatacgt cagcgcttca gtcgcgatat caacaggatc cggcactggt
     3001 tgttgttgta agcgcccttc atcaatcacc acgtttctgc gaagaaaacg tgaagtgtta
     3061 taggcatgat tgcgaatata ttccagaaca aggcagatag tttcaccgtg gtagcgatac
     3121 agcgaaaaca gtaatcggtc ccggatactg agccgggcgc ggtaaagatt ggtgtcaatt
     3181 ttacgaacat cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa ttttttataa
     3241 cccgggatcg tgtcaggatt cagagtatga taaagacaga ttttcatggc tgccggtttc
     3301 cgtcgtgcaa aaaacatgct gattttacga aggaatgcct tactgaacaa ccttgcccgg
     3361 aatacccccc gaaatcagat ggaacaatca gaaaaatgct ctgcagttat ggaataaaga
     3421 aggtggggtg gtacgccggg agggcgatcc gctggaaatg gcgcagtagc tgcaggcctg
     3481 tcatgatgca ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc cgtaaataaa
     3541 ctctgcgctg aaattccttt ttttcattgg agtacctgtg ttgttctgag gtgaacatat
     3601 cacctctgtt caggtagcca aattcagtgt gccacctcaa ggttcttagt gagtcgtttt
     3661 taaaagatat gataatatat cattgtttat gaattgttga tgtttaattt agtaaattat
     3721 atcaaatatg ccatgggttt gctatgttat tcatccagta tctggaaata aaatgcacca
     3781 gctttattgg gttttccttt tgtctctgct tgctaggtgc cagaaacttt aaatgttaaa
     3841 cgaagggggc attttgttca aagccaatca tgttccggta ttaatgtatc atcatgtcag
     3901 tcattgcccc ggactggtta cactttctcc cgtgacattc cggaagcaaa taaaatggtt
     3961 ggctgaaaac aactggaaaa cactgtcatc agatgaactg gagttttttt atcggggcgg
     4021 aaaactcccc cggaaaagcg ttatgttgac atttgatgat ggataccttg acaactggtt
     4081 tcaggtatat cctttattaa aagagtttaa tctcaaagca catatttttt taattaccgg
     4141 ttttattggg aatggtccgg ttcgtcactc tcctggaaag gaatattctc atcgcgattg
     4201 cgaacatcaa atagcgacag ggaatgctga taatgttatg cttcgctggt ctgaagtaaa
     4261 cgaaatgttg caatctggtc tggtcgaatt tcatgttcat acgcatactc atacacgctg
     4321 ggataaaaag ttcagttcac gagaagaaca gtgtaaacat cttcggcagg acttgttgag
     4381 cgggagggaa tatttgaaag aaatgacagg aaaatgcagt aaacatttgt gttggccgga
     4441 aggttattat aacaaggatt atattcaggt cgctgaagaa cttggattct attatttata
     4501 tacaacagaa agaagaatga atgctcctgc taaaggaact acacggatag gaagaataag
     4561 caccaaagaa cgggaaagtt gcgcatggct gaaaagacga cttttttatt atacaacccc
     4621 gtttttttca tcgttgctgg cctttcataa ggggccaaga ttaccggatg attaataatg
     4681 aatatactat ttacggaatc atcaccgaat ataggtggtc aggaattaca ggctgttgct
     4741 caaatgaagg ccctgaagaa aatggggcat tcagttctgc ttgtctgcag ggaaaacagc
     4801 aaaattgctt ttgaagccag taaattgggg attgatatca cattcgcgtt atttcgaaac
     4861 agtcttcaca tccctactgc atggagatta ctcggaatag ttcatggttt tcagcccaat
     4921 gcaatcgttt gtcacagtgg gcatgatagc aatattgttg gtttagtacg gttattcact
     4981 aggaaacatc cattcagaat tatcaggcaa aagacatatt tgacacgaaa aacaaaagtt
     5041 ttttcaataa atcatttttg cgatgaggtg attgttcccg gaacaagtat gaagacacat
     5101 ctggagcagg aaggatgtcg aacccgggtt actgttgtgc ctccaggctt tgatttccag
     5161 aaattatacg ttgattcccg aaacagtttg cctccaaatg ttctttcttg gctggcgtcc
     5221 cgaaggggat gccccgttat tgctcaggta ggaatgttgc gcccggaaaa agggcacgaa
     5281 tttatgttga atttactgtt ccatttaaaa atgaatggac gacagttctg ttggttgatt
     5341 gtggggtctg gttcgcctga actgcgggag catttacagt atcagattga cagtatgggc
     5401 atgcatgatg atgtttttat tgctgacaat gtttttcctg ccgcccccgt atatcgggtt
     5461 gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg gtatggtact ggcagaagca
     5521 tcggcatttt ctgtgcctgt actggccagt cagattggtg gaatccctga tgttattcag
     5581 aataaccaga ccgggacatt gttaccagca ggtaataagc acgcatggat gtgcgccctg
     5641 aatgattttt ttaatgaccc tgggcgtttt tatcagatgg ctcgccaggc aaaacaggat
     5701 atagaagagc ggtttgatat taataaaact gcgttaaaaa tactcacatt agcgaagcac
     5761 aagtaacaat atgttttctg taagtaactt atcatttatc ggtttcctta aaaggattgt
     5821 tttttcctca gattcactcc cggggaagtg ggaacacaga aaattccggt tcatgtacat
     5881 tttgcgatgt gctataaatc cggttgccag tattcgatat tattacgaac tgcgttcctt
     5941 gcagtgcatt gaggatattc tggcaataca acccacgttg ccagcaagaa ttcatcgacc
     6001 ttatttgcat aaaggggggc gagcctggtc ccgggggcaa tatattcttg agcattatcg
     6061 ctttgtgcaa aatttgccag aaaaatattc tgaatttttg tttccacaaa aatcggtatc
     6121 attagtccaa ttcatcggga aagatggaga ggattttgat atccagtgtt ctcccagtgg
     6181 ttttgaccgg gaaggagagt tgatgttgtc attgtttttc aataaaatag tgattgcccg
     6241 gttaacattt tcagtcattc tgactcaaaa tggacacacc gcttttattg gcggattaca
     6301 gggagccccc aaaaataccg gacctgatat tattcggtgc gcaacccggg cctgttatgg
     6361 gcttttccca aaacggatta tttttgaagc attttgtgcg ttgatgaaag cttgtaatgt
     6421 ctctgagtgt ctggcagtca gtgagcacag tcatgttttc cggcaattga gatactggta
     6481 tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc tgggagtctg tagcagggaa
     6541 aacctgtggt gactggtaca aattaccaac acaggtggtt cgaaagccgc taagcaatat
     6601 agctagtaaa aaacgctctg agtatcgaaa acgatacgct ttgctggatt atatccatga
     6661 aactgctatc cgctctttgg atgcatatcc tgtaaactca gaacatcagg acttaaatta
     6721 aggtttgatg tattctctga ataaaatatt aatgatgatt ttggtagggg cattcgcact
     6781 aaataatgaa aaaatacaaa tccgagttta ttcctgaatt taagaaaaat tatctttccc
     6841 ctgtttactg gtttacatgg ttcgttttgg gaatgattgc aggtatttca atgtttcccc
     6901 cttcattcag agatcctgtc ttggccaaaa tagggcgttg ggtgggcaga ttgagcagaa
     6961 aagctcgtcg cagggcgacg attaatttat cgctttgttt cccggaaaag agtgatacag
     7021 aacgggaaat aattgtcgac aatatgtttg ccacagcatt gcaatccata gtgatgatgg
     7081 cagaactggc aattcgtggt ccggaaaagt tccagaaacg agtgttctgg aaaggcctcg
     7141 aaattcttga ggaaattcgg cacaataaca gaaatgtgat ttttctggtt ccccatggct
     7201 ggagcgtgga tattcctgca atgttgctgg cagctcaggg ggaaaaaatg gccgccatgt
     7261 ttcatcagca acgaaatcca gtgattgatt atgtctggaa ttctgtacgg cgtaaattcg
     7321 gggggcgctt acattcccgg gaggatggga taaaaccatt tattcagtca gtacgccagg
     7381 gatactgggg gtattacctt ccagatcagg atcatggtcc tgaatacagt gaatttgctg
     7441 atttttttgc gacctataaa gcgacattac caattattgg acgtctgatg aacatcagtc
     7501 aggctatgat tataccgctt ttcccggttt atgatgaaaa aaaacatttc ctgactattg
     7561 aagttcggcc accaatggat gcatgcattg ccagcgcgga caataaaatg attgcccgac
     7621 aaatgaacaa aacagtggaa attttggtgg ggtcacatcc ggaacagtat atctgggttt
     7681 taaaattgtt aaaaacgcgc aaatcaaacg aagcggaccc gtacccttga aattataaag
     7741 tacaggtatt tccactagtt gtttcttaca ggttaccaat cgaaacacat cccccttccg
     7801 tgtgttgccc gtttggcacg gaggactgtg tagatgcagt tcgttaaatt taatttatgc
     7861 ctaaatagct gcgcttaata ccgctacact tttgccagcc catgtttgcc tccggggaat
     7921 gggctgacgg tttccataaa atggcgaact tttttcaaca gttgccacat tgagcggcac
     7981 tgatgattac gcgttattgt gtcgtgaagt gcctgccata gccgttcaac atgattcacc
     8041 catggcgagt aaaccggctg ataaatgccc ctgaacttcg ggttctcctt cagccagctc
     8101 tgtgtttccc ggcttttgtg gataatgtag ttgtccacga tcagcgtgat ggttttcgca
     8161 cgacggtatg tcgctttaag ccgcttcagc aggctgatga acagcgccga acttttgctg
     8221 ttgccgccca catagctgac tttacctgtc ccgctgtgca gcgctccggc cagataatat
     8281 ttttcattct gtcccggcgt gaccacccgt ttttgctgtc cgcgcagttg ccagtccgca
     8341 ccgattttgg gatgaagatg gatatccact tcatcttcat aaaagaccgg atgctctgcg
     8401 ctgcattcgt ccagtgcttt atggattgct gccatctttt catctttatg cgggtcacgg
     8461 atacgcagag ttggcgcagc ccttcgccac acaatccccg cagacggcaa ccagcggcga
     8521 acggttccgg ca
//
LOCUS       DAJTRD010000142         8435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTRD010000142 DAJTRD010000000
VERSION     DAJTRD010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.142"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..305
                     /locus_tag="OO315_003710"
     CDS             <1..305
                     /locus_tag="OO315_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCR5892465.1"
                     /translation="LIARWELTAEQAVAQQLKNQVSKGNLIDTGFCIFALSKLAMALS
                     STLDSIPLSMQRQFPDLTPRHIDHLKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            277..2187
                     /locus_tag="OO315_003711"
     CDS             277..2187
                     /locus_tag="OO315_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5892466.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            2188..2391
                     /locus_tag="OO315_003712"
     CDS             2188..2391
                     /locus_tag="OO315_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5892467.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2388..3981
                     /locus_tag="OO315_003713"
                     /pseudo
     CDS             2388..3981
                     /locus_tag="OO315_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3971..5476
                     /locus_tag="OO315_003714"
     CDS             3971..5476
                     /locus_tag="OO315_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5892468.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5513..5860
                     /locus_tag="OO315_003715"
     CDS             5513..5860
                     /locus_tag="OO315_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5892469.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5918..6946
                     /locus_tag="OO315_003716"
     CDS             5918..6946
                     /locus_tag="OO315_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5892470.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6998..7384
                     /locus_tag="OO315_003717"
     CDS             6998..7384
                     /locus_tag="OO315_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5892471.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7396..7773
                     /locus_tag="OO315_003718"
     CDS             7396..7773
                     /locus_tag="OO315_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5892472.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7760..8344
                     /locus_tag="OO315_003719"
     CDS             7760..8344
                     /locus_tag="OO315_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5892473.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIPMSGPL
                     TQAFEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            8341..>8435
                     /locus_tag="OO315_003720"
     CDS             8341..>8435
                     /locus_tag="OO315_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5892474.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPA"
BASE COUNT         1960 a         1989 c         2599 g         1887 t
ORIGIN      
        1 tactcattgc cagatgggaa ctgaccgcag aacaggctgt cgcacagcag ttaaaaaatc
       61 aggtgtcaaa aggaaatctt atcgataccg gattttgtat ttttgccctt agtaagctgg
      121 cgatggcttt atccagtacg cttgactcca ttcctttgtc catgcaacga cagttcccgg
      181 atttaacgcc gcgtcatatt gatcatctga aaatccttat tgctaaggga gcgaatcagt
      241 gcgcgcgtgc gggggataaa ttaccggatt tactcgatga atatatcaga acaacaactg
      301 agtaatatga tggtggcagt cactatcgca ctgcagccac tggtcagggt attgccggtg
      361 acggctgtag aatgggccga tcaaaattat tacctgccta aagaatcgtc atacggtgag
      421 ggggaatgga aaacgctgca gtttcaggtc gccatcatga actgcatggg caatgacgag
      481 attcgtacag ttaacctgat taaatctgcc cgtgttggtt atacaaaaat ggtgcttggt
      541 gtgatcgggt attttattga gcacaaatcc cgcaacactc ttctttttca gccgacggac
      601 tcagctgctg aagattttat gaagtctcac gtggaagcaa caattcgtga tgttccttgc
      661 ctgaaaaaac tttctccctg gctgggccgg aaacaccggg ataatacgct cacactgaag
      721 cgtttttcct ctggtgtggg gttctggtgt ttgggtggtg ctgctgccaa aaactaccgt
      781 gaaaaatctg tcgatgtggt ctgctatgac gaactctcct cgtttgaacc ggatgtggaa
      841 aaagaaggtt cgccaacact gcttggcgat aaacgtattg aaggttcggt atggccaaaa
      901 tccatacgcg gttcaacgcc aaaaattaaa ggctcctgtc agattgaaaa agctgcgaat
      961 gaatctgcgc atttcatgcg gttttatgtc ccttgccctc attgcgggga ggagcagtat
     1021 ctgaagtttg gcgatgatgc gacgtcgttt ggcctgaaat gggagaaggg taaaccggaa
     1081 acggtgtatt acctgtgtga acataatggc tgcgtgatcc gtcagtctga acttgatcag
     1141 accggggggc gctggatttg tgacaatacc gggatgtgga cgtgtgacgg tctgacgttt
     1201 ttcagtgctt cgggtaatga aattccgccg ccgcgctcca tcactttcca tatatggacg
     1261 gcgtacagtc cgttcaccac cagggtacag atagtctgtg actggctgga tgcactgaaa
     1321 gatcccaacg gcgtgaaaac ttttgtgaac accacgctgg gcgagacctg ggaagaggcc
     1381 gtgggcgaaa aactcgatca ccaggtactg atggataagg ttgtgcctta cacggcaacg
     1441 gtgcctgtcc gggtggttta tctgacggcg ggcattgact cgcagcgaaa ccgttttgag
     1501 atgtatgtct gggggtgggc tccgggagag gaagcctttc tggtggataa aatcatcatc
     1561 atgggacgtc ctgatgagga agagacgctg ttacgtgtgg atgcggcgat caataaaaaa
     1621 taccgccatg cagacggtac cgaaatgacc atttcccgtg tctgctggga tatcgggggg
     1681 atcgatggcg aaatcgtgta tcagaggtca aaaaaacacg gtattttccg ggtactgccg
     1741 gtaaaaggtg cctctgtcta tggcaagccg gtgatcacca tgccgaaaac ccgcaatcag
     1801 cggggtgtgt atctgtgtga ggtggggacg gataccgcaa aagaaattct ctatgcccga
     1861 atgaaggccg atcccacccc tgtgaatgag gccacatcgt atgccatccg ctttcctgat
     1921 aatccggaga ttttttcgca gacagaggca caacaactgg tggcggagga actggtggaa
     1981 aagtgggaaa aaggaaagat gcgtctgttg tgggataaca aaaagcggcg taacgaagcg
     2041 ctggactgcc tggtgtatgc ctatgcggca ttacgtgtgt ccgtgcaacg ctggcaactt
     2101 gatctggctg tgcagttgca tcccgcaagg atgcaactgc acaaccgacc cttgaagaac
     2161 tggcagcgaa gctgtccgga ggagtgaatg gttacagtcg ctgaactgca ggcgctgcgt
     2221 caggcgcgcc ttgatttatt aaccggtaaa cgggttgtgt cggtacagaa agacggtcgc
     2281 aggatcgaat atacggcagc gtccctgaat gagcttaacc gtgcgatcaa cgatgcggag
     2341 tcggttctgg gtacgacccg acgccgccga ccgctgggag tgaggttatg aaacgaacgc
     2401 ctgtcctgat tgatgtgaac ggcgttccgc ttcgtgagag tctcagctac aacggaggcg
     2461 gcgcaggatt tggcgggcaa atggcagagt ggttaccacc ggcgcagagt gccgatgcgg
     2521 ccctgttgcc tgcattgcgt ctggggaatg cccgggcaga tgatctggtg cgcaataacg
     2581 gtatcgcggc taatgcggtg gccctgcata aggatcatat cgtcgggcac atgtttctta
     2641 tcagctaccg tctgaactgg cgttggctgg ggatgcggga gaccacggca aaaagttttg
     2701 tcggggaggt ggaagcagcc tggtcggaat acgcagaagg aatgtttggc gagatcgacg
     2761 tggaagggaa acgcacgttt acggaattta tccgtgaagg ggtgggcgtg catgccttta
     2821 acggcgaaat ctttgtgcag ccggtctggg atacggaaac cacgcagtta ttccgtacgc
     2881 gttttaaagc cgtgagtccg aaacgggtgg acacgccagg acacggtatg gggaaccact
     2941 ttctgcgggc cggggttgag gtcgatcggt atgggcgggc gattgcgtac catatctgtg
     3001 aggatgattt tccgttctct ggtgccggac gctgggagcg gatcccgcgt gagcttccca
     3061 ccgggcgtcc ggccatgctg catatttttg agccggtgga ggacgggcag acccgtgggg
     3121 ctaatcagtt ttacagcgta atggagcgga tgaagatgct ggactccctg caggccacgc
     3181 agcttcagtc ggccattgtg aaggcgatgt atgcagccac aatcgaaagt gaacttgata
     3241 ccgaaaaggc atttgaatat atcgcggggg caccgcaagg tcagcaggat aatccgctta
     3301 ttaatattct ggagaagttc acccgctggt atgacacgaa taacgtgacg ctgggcggtg
     3361 ttaaaattcc gcaccttttt tcctggcgat gcgctgaacc tgcagacagc gcaggattca
     3421 gacaatggat tttcggcgct tgaacaggcg ctgctgcggt atattgcagc cggtcttggc
     3481 gtttcctacg aacagttgtc ccgtgattac tcgaaggtca gttactcaag tgcccgcgcc
     3541 tccgccaatg agtcgtggcg ctattttatg gggcggcgaa aatttattgc ggcccggctg
     3601 gccacgcaga tgttttcctg ctggctggaa gaggcgcttc ttcgggggat tattcgtccg
     3661 ccacgggcgc gttttgattt ttaccaggcg cgttcagcct ggtcacgggc agagtggatt
     3721 agctccggaa gaatggccat tgacgggctc aaggaggtcc aggaatcggt gatgcgcatt
     3781 gaggccggac tgagcacgta tgagaaagaa ctggcactga tgggcgaaga ttatcaggac
     3841 attttccgcc agcaggtcag ggaatccgca gagcgcgaaa aagccggact ctcacgtccg
     3901 gtgtggatag cccaggcgta tcagcagcag atagcggaga gtcgcaggcc ggaagaggag
     3961 acaacatcac gtgagacgta atctttcaca tattatcgct gcggcattca atgagccgct
     4021 gcttctggag cccgcctatg cgcgggtttt cttttgcgcg ctcgggcgtg agatgggcgc
     4081 agcaagcctt tcggtaccgc aacagcaggt acagcttgac gctcccggga tgctggctga
     4141 aacggacgag tacatggccg gaggcaaacg acctgcccgt gtttaccggg tggtgaacgg
     4201 tattgctgta ctgccggtga ccggcacgct ggtacaccgg ctgggtggca tgcggccatt
     4261 ttccggaatg actggctatg acggcattgt cgcctgtctt cagcaggcaa tggcggacac
     4321 tcaggtacgg ggcgtactgc tggacattga cagttctggc gggcaggcca ccggcgcgtt
     4381 tgactgcgct gacatgattt accgtctccg gcagctgaag cctgtctggg cactgtgcaa
     4441 tgacacggcc tgttcagccg ccatgttact ggcatcggcc tgctcccgac ggctggttac
     4501 ccagacatcc cgtatcggct ccattggggt gatgatgggc catgtcagct atgccggtca
     4561 tctggcacag gccggtgtgg atatcacgct gatttacgcc ggatcgcaca aggtggatgg
     4621 caatcagttt gaatccctgc cagcaaaggt tcgccaggac atgcagcagc gcattgatgc
     4681 ggcgcgccgg atgtttgctg aaaaagtggc gatgtatacc ggtttatctg tggatgcggt
     4741 tatgggaaca gaggccgccg tttttgaagg tcagtccggt attgaggccg aactggcgga
     4801 tgaattaatc aatgcgtcgg acgccatcag cgtgatggct gcgacgctga acacacacga
     4861 tacaggaggc actatgccgc aattaactgc aacggaggct gccgcgcagg agaaccagcg
     4921 agtgatgggg atcctgacgt gtcaggaagc gaaaggacgt gaacaactgg ccacgatgct
     4981 ggcagggcaa cagggtatga gcgttgagca ggcccgggcg attctggccg tggcggcacc
     5041 gcaacaatct ggtgtatctg cgcagagtga agccgatcgc attatggcat gtgaagaggc
     5101 gaaaggacgt gaacaactgg cggcaacgct ggcggcgatg ccggagatga cggtggaaaa
     5161 agcccgtccg attctggctg cttcaccgca ggcgaatgcc ggaccctcac ttcgtgatca
     5221 gattatggcc ctggatgagg caaaaggggc agaggcgcag gctgaaaaac tggcggcctg
     5281 tccggggatg accgtggaga atgcccgggc tgtactggcg gcagcctcag gcagggcaga
     5341 accggtttct gcatccacaa ccgccatgtt tgaacatttc atggcgaacc attcaccggc
     5401 agcggtacag ggtggcgtgc cacagacgtc agcagacggt gatgcggacg tgaaaatgct
     5461 catggccatg ccatgaagtc agtgctgacc atcaatatga ggtttttaca atatggtaac
     5521 gaaaaccatc actgaacagc gtgcggaagt acgtattttt gccggtaatg atccggccca
     5581 taccgccaca ggctgcagcg ggatttcctc ggcaacaccg gctctgacgc ccctgatgct
     5641 ggatgaggat accgggaaac tggtggtctg ggacggacag aaggcaggcc atgtggttgg
     5701 catactggta ctgccgcttg aaggtacaga gacggtgctg acgtattaca agtcggggac
     5761 ctttgcgacg gaggccatcc actggcctga gagagtggat acacacaaaa aggcgaatgc
     5821 ctttgccggc agtgccctga gtcacgcagc gctgccgtaa cattttatca ggtcactgcg
     5881 gtagcctgac tgatttctga atgaaaggaa ctgatttatg ggattgttta cgacccgcca
     5941 gttactcggt tataccgaac aaaaagttaa atttcgtgcg ctgtttctgg agctgttttt
     6001 ccgccgtacg gtgaatttcc ataccgaaga ggtaatgctg gacaaaatta ccggaaaaac
     6061 gccggtggcg gcctatgtct ccccggttgt tgaaggaaag gtgttgcgtc accgtggcgg
     6121 tgaaactcgt gtactgcgcc cgggctacgt taagccgaaa cacgaattta attaccagca
     6181 ggcggtggag cgtcttcccg gtgaagatcc ggcccagctg aacggcccgg cttaccgtcg
     6241 cctgcgtatc atcaccgata acctcaaaca ggaagagcac gccattgtcc aggtggaaga
     6301 gatgcaggcg gtgaatgccg tgctgtatgg caaatacacc atggaaggag agcagtttga
     6361 aactgttgag gtcgattttg gccggtcggt gggaaataac attacgcagg caggagggac
     6421 ggagtggagc gctcaggacc gcgatacctt tgatccgacg catgatctgg atgcgtactg
     6481 tgattttgct tccggcacca tcaatattgc cattatggat ggtaaggtct ggcgtctgct
     6541 gaatggcttt aagctgttcc gcgaaaaact ggatacccgt cgtggctcaa attcacagct
     6601 ggaaacggca gtgaaagacc tgggggcggt ggtgtccttc aaagggtatt atggtgatct
     6661 ggcaatcgtg gtggcaaaaa cgtcctatgt ggcagaggac ggtaccgaaa aacgttatct
     6721 gccggacggc acactggtac tgggaaatac ggccgcagag ggtattcgtt gctacggtgc
     6781 cattcaggat gcgcaggcat tgtctgaagg cgtggtggcg tcttcccgtt atccgaaaca
     6841 ctggctgacc gtgggtgatc cggcgcgtga attcaccatg acgcagtccg cgccgctgat
     6901 ggtgctgccg gatccggatg agtttgtggt ggtgcaggtg aaataatccg tgagcgggag
     6961 ctaaatgctc ccgtttttca ttcaatcagg agcgggtatg gctacgaaag aagaaaacct
     7021 gaaacgtctt tgtgagctgg ctgagaggct ggggcgtgaa ccggatgtct ccggtagtgc
     7081 ggcagatatt gcgcagcgag tggcggaact ggaggaagag cttggagatg cgggggaggt
     7141 cgcagagccg gatacgtact caccatcgaa agatgatccg accgcgcata aaggcgagtc
     7201 agttcctgaa cagccaggca ttgttaccgg agacgaaacg gggcaggtta cggtggtggc
     7261 actggcgaca ctgcatacaa aatgtaatgg ttcagtgata tttgtttcac cgggaacgtc
     7321 gtttcgtgtt tctgctgagg tggctgccag catggcggca ggtggtctgg caaaaaggca
     7381 gtaacaggag atgtcgtgga cggtttcgat aatctgtttg atgctgcgct tgccggtgtc
     7441 aatgaagtca ttctccgtga tatggggatc agcgccgtca ttacgtccgg agaacttgag
     7501 gggacacacc tgacaggggt ttttgatgat cccgaaagta tttcttttgt tgccgggggg
     7561 atccggcttg aggattcctc accgtgcctg tttgtgaaaa cggcagatat atcgcagtta
     7621 cgccgtcagg atacgttaac tatcggggat gattctttct ttgtggatcg tattactccg
     7681 gatgacgggg gatgctgtta tatccggtta aggcggggca gtcctgcccc acagaataat
     7741 gccagaatga ggcgatatga tgaagggact tgagaatgcc atccgcaatc tgaacagcct
     7801 cgatacccgc atggtgccac agaccagcgc atgggcggta aaccgtgtgg cggcaaagat
     7861 tgtttctgtc gccacacggc aggttgcgca gaataccgtt gccggggata accaggtgaa
     7921 aggtattccc ctgaaaacgg tcagggagcg tgtcaggctg cttaaagcca gcccctcagg
     7981 aaaaatgtat gcccgcatgc gtgttaaccg gggtaacctg cccgccatta agctgggtac
     8041 cgcacaggtc agactggccc gctcccggca tggcagcaac tcacgtcatc gtggcagcgt
     8101 actgaaagtg gggaaatacc ttttccggga tgcgtttatt cagcaactgg cgaatggtcg
     8161 ctggcatgtg atgcggcgta ttgacggcaa aaatcgttac cccattgatg tggtgaaaat
     8221 cccgatgtcc ggaccgctga cacaggcatt tgaagatgcc cgcgaccgca tcattgctgc
     8281 ggaaatgccg aaacagctgg ggtatgccct gaaacaacaa ctgaggttac atctgagtaa
     8341 atgaaccgac acacacaaat ccgtcagagc gtgctggcac gccttcggga acagtgtgga
     8401 gacagcgcca cgttttttga cgggcttccg gcatt
//
LOCUS       DAJTRD010000143         8374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTRD010000143 DAJTRD010000000
VERSION     DAJTRD010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.143"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..955
                     /gene="espL1"
                     /locus_tag="OO315_003721"
                     /pseudo
     CDS             1..955
                     /gene="espL1"
                     /locus_tag="OO315_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1127..1234
                     /locus_tag="OO315_003722"
     CDS             1127..1234
                     /locus_tag="OO315_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892475.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1287..2045)
                     /locus_tag="OO315_003723"
     CDS             complement(1287..2045)
                     /locus_tag="OO315_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5892476.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2332..3261
                     /gene="pfkB"
                     /locus_tag="OO315_003724"
     CDS             2332..3261
                     /gene="pfkB"
                     /locus_tag="OO315_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5892477.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3362..3652
                     /gene="ghoS"
                     /locus_tag="OO315_003725"
     CDS             3362..3652
                     /gene="ghoS"
                     /locus_tag="OO315_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5892478.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3758..4618
                     /locus_tag="OO315_003726"
     CDS             3758..4618
                     /locus_tag="OO315_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5892479.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4659..5195)
                     /locus_tag="OO315_003727"
     CDS             complement(4659..5195)
                     /locus_tag="OO315_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5892480.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5342..6010
                     /gene="hxpB"
                     /locus_tag="OO315_003728"
     CDS             5342..6010
                     /gene="hxpB"
                     /locus_tag="OO315_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5892481.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6173..6763
                     /locus_tag="OO315_003729"
     CDS             6173..6763
                     /locus_tag="OO315_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5892482.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6896..8286
                     /gene="tcyP"
                     /locus_tag="OO315_003730"
                     /pseudo
     CDS             6896..8286
                     /gene="tcyP"
                     /locus_tag="OO315_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT         2173 a         2004 c         2007 g         2190 t
ORIGIN      
        1 gctcagacct gggctgatag gtatgttcgc aactgtgcaa ttgctattct gcccctatta
       61 cctgtgggag ttctaggggc tattttcgcg ggtattgcat cacgaatgcc atttgcccct
      121 atacatccat cggcaatgtt gttaataatg gctacaggcc agactcaaca gcttattaca
      181 ttattcaaac agttgcccat actccctgaa aaagaaatca ttgaaataat aactgcgcag
      241 aatagcgttg gtacacctgc tttatttttg gctatgatga acggacatac tgacaacgtg
      301 aaaatattta tgcaagaaat tcagtcactg gtagataatc acatcattca tgaagataat
      361 ctggttaaat tactgcaaac taaaagtgct aacgaaacac ctggacttta tatctccatg
      421 ttgtatggat tcgatgaaat aatcgatatc tttctgaata cattaaccac tccaatagca
      481 ctaagagctt ttaaacaaaa aactggtgat gagtatttta gccatgaaaa tacatgatgg
      541 tgagccagga ttatacgccg caatggaaaa taatcaccct ttgtgtgtca cacggttcct
      601 ctctaaaatt aatggcatcg cctttaaata caagttgagc aaagctaaca tcatggattt
      661 attaaaagga gctacagcac agggaacccc tgcattatac atcgccatga gcaagggtaa
      721 tgaagacgtc gtgttatctt atatatcgac gctgggtgct tttgcaaaaa aacattcttt
      781 tagtcaacat cagttattta cactattggc cgctaaaaat catgacaaca tgtcagctgt
      841 tcatatagcc attcatcata agcattataa aactgtagaa acatattatg ctgctataaa
      901 tgtcatcagc caaagcctga gttttagtgc tgatgaaata aagacgtatt tataaccagc
      961 tatttacgat atatatcgat acataatttt atttttttca taaaacgcag aaacagtgac
     1021 catgtgcaca acgccacccg acggctgtaa taacaaaaaa tcccttgatg cctgtccctt
     1081 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggtatgc cgactaaacg
     1141 ctttgataaa aaacactgga agatggtggt ggtgctactg gcaatctgtg gcgctatgtt
     1201 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
     1261 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
     1321 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtgac gttgtaagcc
     1381 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
     1441 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
     1501 catgcataag ccccggaggc atagcccagc ggctgggttt cactggcatt gtcggtatat
     1561 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
     1621 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
     1681 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
     1741 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
     1801 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
     1861 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
     1921 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1981 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     2041 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     2101 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     2161 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     2221 atatatattt atctgcaaaa ttttaaataa agctctaata aatcatattg ttaatttctt
     2281 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     2341 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     2401 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     2461 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     2521 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     2581 gaagccaaag actggacccg gcagaattta cacgtacatg tggaagcaag cggtgaacag
     2641 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     2701 caagttctgg aaattgaatc cggggccatc ctggtcataa gcggaagcct gccgccaggt
     2761 gtgaagctgg aaaaattaac ccaactgatt tccgctgcgc aaaaccaagg gatccgctgc
     2821 atcatcgaca gttctggcga agcgttaagt gcagcactgg cgattggtaa catcgagttg
     2881 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccagac
     2941 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     3001 tcccttggtc cacaaggagc gctgggtgtc gatagtgaaa actgcattca ggtggtgcca
     3061 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     3121 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     3181 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     3241 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     3301 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     3361 tatggccagt ggcgatcttg tccattatgt cataaccgtc atgttgcatg aggatacatt
     3421 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     3481 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     3541 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     3601 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     3661 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     3721 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     3781 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     3841 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     3901 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3961 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     4021 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     4081 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     4141 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     4201 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     4261 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     4321 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     4381 tttcgaccct gcctgcgact ggggtgaccg agagtgcgac ctagcgatgt taccgctgca
     4441 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     4501 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caagattatt
     4561 tggcggtcag catttggtta ttgctcagca gtcattggat agattattag cagcatgata
     4621 tgggttgagg ataatggccg ctccgtgcgg ccttttgatt aaataaaccc aagcagagaa
     4681 aagaacacat aacctgcagc aatacaaata accggcagga tatatagtga gaaaaactgc
     4741 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     4801 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     4861 gcctggcgac tcatccgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     4921 aaaatcaccc agaacataac gttcacacca ccattaaaat taggggtcgg ggagttatac
     4981 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagcaaag
     5041 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     5101 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     5161 aaaacagcaa tgcgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     5221 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     5281 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     5341 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     5401 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtagatat
     5461 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     5521 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     5581 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     5641 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     5701 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     5761 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     5821 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     5881 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     5941 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     6001 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     6061 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     6121 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     6181 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     6241 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     6301 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     6361 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     6421 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     6481 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     6541 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctgg ttccccaaaa
     6601 gggcaaccaa ctggaacgtt ttatctgcat ggcattattt gtctggtcgg tatggatgcc
     6661 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaatgatca ataccttgca
     6721 gatccagttt catcggctta ttaagcatca ggttgaatac taaaaaggca aaaatcacct
     6781 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     6841 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     6901 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6961 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     7021 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     7081 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     7141 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     7201 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     7261 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     7321 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatcagag
     7381 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     7441 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     7501 gaaactgctg aaggatgatg cgccgaaagg ggaacgcgtc ttaaccgcta tcgataccct
     7561 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     7621 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     7681 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gctgtgcatg gcattctgct
     7741 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     7801 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     7861 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     7921 tcagaacggc tgcgccggtt tgtatccggc aatgctgcgg tgatggttgc gcctacggtt
     7981 ggcattaacc cgctggaccc gatgtggatt gcgacgctgg tcggtattgt taccgttagt
     8041 tccgcaggcg ttgccggtgt cggtggtggt gcaactttcg ccgcactgat tgtgctgcct
     8101 gcgatgggcc tgccagtaac cctggtggcg ctgttaatct ccgttgaacc gcttatcgac
     8161 atgggccgta cggcgctaaa cgttagtggc tcgatgacag ctggcacgct gaccagccag
     8221 tggctgaagc aaaccgataa agccattctg gatagcgaag acgacgccga actggcacac
     8281 cgttaattct tatgctggca acggtccgtt ttgtataggg gccgttgctt tactttttaa
     8341 atgttccatc gcggcgatgc atactgactc atat
//
LOCUS       DAJTRD010000144         8333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTRD010000144 DAJTRD010000000
VERSION     DAJTRD010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.144"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..159
                     /locus_tag="OO315_003731"
     CDS             <1..159
                     /locus_tag="OO315_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892483.1"
                     /translation="ISESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGE
                     DVEPGDDF"
     gene            complement(168..566)
                     /gene="vapC"
                     /locus_tag="OO315_003732"
     CDS             complement(168..566)
                     /gene="vapC"
                     /locus_tag="OO315_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5892484.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(566..793)
                     /gene="vapB"
                     /locus_tag="OO315_003733"
     CDS             complement(566..793)
                     /gene="vapB"
                     /locus_tag="OO315_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5892485.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            875..1246
                     /locus_tag="OO315_003734"
     CDS             875..1246
                     /locus_tag="OO315_003734"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5892486.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1267..6128
                     /gene="traI"
                     /locus_tag="OO315_003735"
                     /pseudo
     CDS             1267..6128
                     /gene="traI"
                     /locus_tag="OO315_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6148..6894
                     /gene="traX"
                     /locus_tag="OO315_003736"
     CDS             6148..6894
                     /gene="traX"
                     /locus_tag="OO315_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5892487.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6949..7509
                     /locus_tag="OO315_003737"
     CDS             6949..7509
                     /locus_tag="OO315_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5892488.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7640..7852
                     /locus_tag="OO315_003738"
     CDS             7640..7852
                     /locus_tag="OO315_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5892489.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8096..>8333
                     /locus_tag="OO315_003739"
     CDS             8096..>8333
                     /locus_tag="OO315_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5892490.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2085 c         2671 g         1664 t
ORIGIN      
        1 atcagtgaat ccggtgaagt ggtggatatg gccgcttatg aggcatggca gcaggaaaat
       61 catccggaca cctggcagca gatgcagcgt cgtgaagagg tgaacatcaa tgtgcaccgg
      121 gaacgcgggg aggatgttga gccgggagat gatttctgat gaacaggtca gctccagtct
      181 tcagttctca ggccgcccac acgttcaaat tcccgggtgt tattagtcac aataatcagc
      241 ccccgactgc gggcatgacc tgcgatcatt tgatcaaatg gcccgacagg gcgtccctga
      301 cgggcaagtt ctgctcttat ctggccggtg tgtgtggctg cagcagcgtc gtaatccaga
      361 acatcaatgc gggaaacaaa tccctcgatc acagcgagat tacgttcagg catctggctt
      421 ttttctgcac catatatcag ctccatcagg gtgaccgaac tgatacacat tttcccctgg
      481 ttcaggttaa aacgctccct gacgctggcg ggtttgttct ttatcgtaaa aatgcagatg
      541 ttggtatcga gcataaactt cagcatcaga atgactccct ttcttgcatg ccgggctgtt
      601 ccctgttatc cataaaatcg gtgctgacgc tgtgtccgtc gaaccattcg tcccatgtct
      661 ctccggctgg cgtaatgatt ctggttcgtc cgacagcaat cacttcaacg cgttttacgt
      721 tttccggcaa tgcaaccgct tttggcagtc tgaccgcctg gctgcggttg ctgagaaata
      781 cggtggtttc cattatttgc ctccttatgt ggatatgtct tgtgtatatc tattgtggga
      841 ttgataccgg gatatgtcaa agggatatac gtttatgatg agtatcgcgc aggtcagatc
      901 ggccggaagt gccggtaatt attataccga caaggataat tactatgtgc tgggcagcat
      961 gggagaacgc tgggccggcc agggggctga acaactgggg ctgcagggca gtgtcgataa
     1021 ggatgtcttt acccgccttc tggagggcag gctgccggac ggggcggatt taagccgcat
     1081 gcaggatggc agtaacaggc atcgtcccgg ctacgacctg accttctccg ctcccaaaag
     1141 tatctccatg atggccatgc tcggggggga caaacgtctt attgaagcac ataaccaggc
     1201 cgtggatttt gctgtccgtc aggtggaggc cagtgcctcc acctgacgga cggacagtca
     1261 gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accacgacac cagtcgcgat
     1321 caggaaccac agttacacac gcatgcggtg gtgactaatg ttacgcagta taacggcgaa
     1381 tggaagacac tgagcagtga caaagtgggg aaaacggggt tcattgagaa tgtgtacgct
     1441 aatcagattg cctttggcag gctctaccgg gaaaaactga aagagcaggt tgaggcgctg
     1501 ggctatgaaa ctgaagtggt cggtaagcac ggtatgtggg aaatgccggg tgtaccggtg
     1561 gaggcctttt ccggacgctc acagactatc cgggaggccg tcggggaaga cgcctcgctg
     1621 aaatcccggg atgtggcggc gctggatacg cgtaaatcca aacagcacgt cgatcctgaa
     1681 gtcagaatgg ccgagtggat gcagacgctg aaggaaaccg ggttcgacat ccgggcatat
     1741 cgtgacgcag cggaacaacg ggcatatacc cgcacgcaga cacccggacc cgcttcacag
     1801 gacgggccgg atgtgcagca ggcggtgaca caggccattg ccggattaag tgaacgcaaa
     1861 gtgcagttta tgtatacgga cctgctggcc aggacggtcg gcatactgcc gccggaaaat
     1921 ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga gcagcttatc
     1981 cccctcgatc gtgagaaggg gctgttcacg ttcgggattc atatgctcga tgagctgtca
     2041 gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca tccggagaaa
     2101 agtgtccccc ggacggccgg ttacagcgat gccgtcagtg ttctggcaca ggaccgtccg
     2161 tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg ggtggctgaa
     2221 ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc tgaccgtcgc
     2281 tcgcagatga acctgaagca ggatgaacgg ctgtccggtg aactgataac cggacgtcgt
     2341 cagttgctgg aaggaatggc cttcacgccg ggcagtaccg ttatcgtcga ccagggcgaa
     2401 aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca taacgtacag
     2461 gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcactgat ggccatgaag
     2521 gatgccgggg tgaacacata tcgctggcag gggggagaac agcgaccggc caccatcatc
     2581 agtgaaccgg accgtaatgt cggctggccg gagattttgc ggccagcgtg aaagccggag
     2641 aagagagcgt ggcacaggtc agcggagtac gggaacaggc catactgaca caggccattc
     2701 gcagtgagct gaaaacacag ggcgtgctcg gacacccgga ggtaaccatg accgccctgt
     2761 caccggtctg gctggacagc cggagccgtt atctgcggga tatgtaccgc ccgggaatgg
     2821 tgatggagca gtggaacccg gagacacgca gtcatgaccg ctatgtgaca gaacgtgtga
     2881 cggcgcagag tcacagcctg accctgcgga atgcgcaggg cgaaacgcag gtggtgcgta
     2941 tttcttccct ggacagcagc tggtcgctgt tccggccgga aaaaatgccg gtggcagacg
     3001 gtgagcgtct gagggtgaca gggaaaattc ccggactccg cgtctccggc ggtgaccgcc
     3061 tgcaggtggc atccgtcagt gaagatgcga tgacggttgt tgtgccggga cgggctgaac
     3121 cggccaccct gcctgtgagc gattcaccgt tcactgcact gaagctggag aacggctggg
     3181 tggaaacgcc cgggcattcc gtcagcgaca gtgcgacggt ttttgcctcc gtcacacaga
     3241 tggcaatgga caatgccacc ctgaatggtc tggcccgcag tggtcgtgat gtccggctgt
     3301 attcctcact ggatgaaacc cgtactgcgg aaaaacttgc ccgccatccc tcctttacgg
     3361 tggtttctga gcagataaag gcgcgtgccg gtgagacatt gctggaaacc gctatcagtc
     3421 tgcagaaagc cgggcttcac acgccggcac agcaggccat tcatctggcc cttcctgtgc
     3481 tggaaagtaa aaacctggcc ttcagcatgg tggacctgct gacagaggcg aagtcgtttg
     3541 ctgcagaagg aaccggtttt gctgacctgg gaggggaaat caatgtgcag ataaaacggg
     3601 gtgatttact gtatgtggat gtggcaaaag gctatggcac aggcctgctg gtttcccgtg
     3661 cgtcgtatga ggcagaaaag agcattcttc gccatattct cgaaggtaag gaggcggtca
     3721 cgccgctgat ggagagagta cctggcgaac tcatggagaa actgacatca ggacagcgcg
     3781 ccgccacccg tatgatactg gaaacgtctg accgtttcac ggtggtgcag ggctatgccg
     3841 gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc cgtgaacatg ctgccggaga
     3901 gtgaacgtcc ccgtgtcgtg gggctggggc ccacacaccg tgcggtcggg gagatgcgca
     3961 gcgccggcgt ggatgcacag acactggcgt cctttctgca tgacacgcag ctgcagcagc
     4021 gcagcggaga aactccggat ttcagcaaca cgctgttcct tctcgatgag agctctatgg
     4081 tgggcaatac cgacatggca cgggcatacg ccctgattgc ggccggtggc ggtcgggctg
     4141 tggccagcgg tgacacggac cagctgcagg ccatcgcgcc cggtcagcct ttccgtctcc
     4201 agcagacgcg cagtgctgcc gatgtggtca tcatgaagga gattgtgcgt cagacgccgg
     4261 aactgcggga ggcggtatac agcctgatta accgggatgt ggaaagggcg ctgtccgggc
     4321 ttgagcgtgt gaaaccgtct caggtgccac gtctggaggg cgcatgggca ccggagcact
     4381 ccgtgacgga gttcagtcac agccaggaag cgaaactggc agaagcgcag cagaaggcga
     4441 tgctgaaagg cgaggctttt ccggatgtcc ccatgacact gtatgaagcc attgtccgcg
     4501 actataccgg caggacaccg gaagcacggg agcagacgct gattgtcacg cacctgaatg
     4561 aggaccggcg cgtactgaac agcatgattc atgatgcacg ggaaaaggcc ggagaactgg
     4621 ggaaggtgca ggtcatggtg cctgtcctga acacagcgaa tatacgcgac ggggagctgc
     4681 gtcgtctctc cacctgggag aataacccgg atgcccttgc cctggtggat aatgtgtatc
     4741 accggattgc cggtatcagt aaggatgacg ggctgataac cctgcaggat gcggaaggta
     4801 acacgcggct gatttcgccc cgggaggcgg tggctgaagg cgtcacgctg tacaccccgg
     4861 acaccatccg ggtggggacc ggtgacagga tacgcttcac gaagagtgac cgggagcgcg
     4921 gttatgtggc caacagcgtc tggacggtga cagcggtttc cggtgacagt gtcacacttt
     4981 ctgacggtca gcagacccgt gtgattcgcc ccggtcagga gcgggcagag caacatattg
     5041 acctggccta tgccatcacc gcccacggtg cacagggggc gagtgaaacc tttgccatcg
     5101 cgcttgaagg cacggaaggt aaccggaaac tgatggccgg ctttgagtca gcctacgtgg
     5161 ccctgtcgcg tatgaagcag catgtgcagg tgtacaccga taaccgtcag ggctggacgg
     5221 atgccatcaa caatgccgta cagaaaggaa ctgcccacga tgtgtttgag ccgaaaccgg
     5281 accgggaggt catgaatgca gagcggctgt tcagtacggc gcgggagctg cgggacgtgg
     5341 cggcaggccg tgccgttctc cgtcaggcgg ggctggccgg gggagacagt cctgcacggt
     5401 ttattgctcc gggacgtaaa tatccgcaac cgtatgtggc actgccggcg tttgaccgta
     5461 acggcaagtc cgccggtatc tggctgaacc cgctgaccac ggatgacgga aacgggctgc
     5521 ggggattcag cggtgaaggt cgggtaaaag gcagcgggga tgcgcagttc gtggccctgc
     5581 agggcagccg taacggagaa agcctgctgg ctgataatat gcaggatggc gtccggattg
     5641 cccgtgataa tcctgacagt ggtgtggtgg taagaatcgc cggtgaaggc cgtccgtgga
     5701 atcccggtgc catcaccggt ggccgcgtgt ggggggatat cccggacaac agcgtccagc
     5761 cgggagccgg aaatggtgaa ccggtcacgg cagaggtact ggcacagcgg caggctgaag
     5821 aggccatccg ccgtgaaacg gaacgtcgcg cagatgaaat tgtccgtaaa atggcagaga
     5881 acaaacctga cctgccggac ggcaaaacag agcaggctgt cagggaaatt gccgggcagg
     5941 agcgtgaccg ggctgccata actgaacggg aagccgcgct gccggagagt gtactgcgtg
     6001 aaccacaacg ggtgcgggaa gcggtccggg aagttgcccg ggaaaatttg ctgcaggagc
     6061 gtctgcagca gatggagcgg gatatggttc gtgacctgca gaaagagaaa accccgggcg
     6121 gagactgata caggaagaca aacactgatg acaacggata acacgaacac gacacgtaac
     6181 gattcactgg ctgcacggac cgatacctgg ttgcagtcat ttctggtctg gtcacccgga
     6241 cagcgggata tcatcaaaac ggtggcactg gtgctgatgg tactggacca catcaatctg
     6301 atattccagc tgaagcagga atggatgttt ctggccgggc gaggagcctt tccgcttttt
     6361 gccctggtgt gggggctgaa tctgtcccgt catgcgcata tccggcaacc ggccattaac
     6421 cggctgtggg gatggggaat tattgcccag ttcgcatatt atctggcagg ctttccttgg
     6481 tatgagggga atatcctgtt tgcctttgca gtggcggcac aggtgctgac gtggtgtgaa
     6541 acgcgcagtg ggtggcgtac agcagcggcc atactcctga tggccctgtg ggggcctttg
     6601 tccggcacca gttacggtat tgccgggttg ctgatgctgg cagtcagcaa ccggctgtac
     6661 cgggcggaag acagagcgga acgtctggcg ctggtcgcct gcctgctggc tgttattccg
     6721 gcgcttaacc ttgccaccag tgatgcagcg gcggtggccg gtcttgtgat gacggtgctg
     6781 accgttggtc tggtgttgtg tgccggaaaa tcattacccc gtttctggcc cggagacttt
     6841 ttcccgacgt tctatgcctg tcatctggct gtgctgggcg ttctggcgct gtgacgggtg
     6901 tggtatcttt ggccgcaaga ggatgattcg tcagaggcag aacacagcat gacagagcag
     6961 aagcgaccgg tactgacact gaagcggaag acagagggaa cagcgcctgt ccgcagccgg
     7021 aaaaccatca tcaatgtcac cacgccacca aagtggaagg tgaaaaagca gaagctggcc
     7081 gagaaagcgg cccgtgaagc agagctggcg gcaaaaaaag cgcaggccag acaggcgctg
     7141 tccatttatc tgaacctgcc cacgctggat gaggctgtga ataccctgaa gccctggtgg
     7201 ccgggattat ttgacggtga cacgccccgg cttctggcct gcggtatccg ggacgtgtta
     7261 ctggaagacg tggcgcagcg gaatatcccg ctctcgcata aaaaactgcg cagggcgctg
     7321 aaggccatca cccgttcaga aagctatctg tgtgccatga aagccggtgc ctgccggtat
     7381 gacacggaag ggtatgtgac ggagcatatt tctcaggagg aggaagcgta tgcgggagcg
     7441 cgtctggcga aaattcgtcg ccagaaccgg ataaaggcag aacttcaggc cgtgcttgat
     7501 gagaaataaa aaaagcctcc cccagagaga ggaggcaggg aaataaggct taaaaggaaa
     7561 taacttccgc ttccgaaagg aagccagtcc actgaattat ttatcacaat aatactgaca
     7621 actaaagggg gacaagaaaa tgaatggatt cagaaacagt tcacggaacg gccaggtctg
     7681 gcgttaccag cgtgccggcg ggcgggccgt tattctggaa gtcagtggac gctggatgga
     7741 agcggcagaa gcatggcgac gggctgcctg tgttgcgcct aggacagact ggcagcagtt
     7801 tgcccggaaa agggctgaac actgccaccg gcgctgccgg ggaagggtgt aacgaaaaaa
     7861 gcagccttca ttactctagg agtcagaagg ctgaaaggag tttatgagat ttgcttctta
     7921 atatccggtc gggcagtatg accggacagt tctgagtagc acagagtctt ttccgcatta
     7981 agtcgatttt ttatattttg tgattagttt cacaaaataa ggtgttattc aatgtgtgct
     8041 gcactattca ggggggctga aatacaggtg gcatttatct gatatggaga ataacatgag
     8101 gaaatatatt ccactggcat tatttatctt ttcatggccg gtattgtctg cagatattca
     8161 tggacgggtt gttcgtgtac tggacggtga taccatcgag gtcatggact cactgaaggc
     8221 ggtgcggatc aggctggtaa atattgatgc accggaaaag aaacaggatt atggccggtg
     8281 gtcaacagac atgatgaaat cactggtcgc aggaaaaacg gttaccgtca cat
//
LOCUS       DAJTRD010000145         8272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTRD010000145 DAJTRD010000000
VERSION     DAJTRD010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.145"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(98..2260)
                     /gene="uvrD"
                     /locus_tag="OO315_003740"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(98..2260)
                     /gene="uvrD"
                     /locus_tag="OO315_003740"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5892491.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(2344..3060)
                     /gene="yigB"
                     /locus_tag="OO315_003741"
     CDS             complement(2344..3060)
                     /gene="yigB"
                     /locus_tag="OO315_003741"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5892492.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(3060..3956)
                     /gene="xerC"
                     /locus_tag="OO315_003742"
     CDS             complement(3060..3956)
                     /gene="xerC"
                     /locus_tag="OO315_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5892493.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(3953..4660)
                     /locus_tag="OO315_003743"
     CDS             complement(3953..4660)
                     /locus_tag="OO315_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5892494.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(4657..5481)
                     /gene="dapF"
                     /locus_tag="OO315_003744"
     CDS             complement(4657..5481)
                     /gene="dapF"
                     /locus_tag="OO315_003744"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5892495.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(5518..5721)
                     /locus_tag="OO315_003745"
     CDS             complement(5518..5721)
                     /locus_tag="OO315_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5892496.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            5910..7388
                     /locus_tag="OO315_003746"
                     /pseudo
     CDS             5910..7388
                     /locus_tag="OO315_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7385..>8272
                     /locus_tag="OO315_003747"
     CDS             7385..>8272
                     /locus_tag="OO315_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5892497.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEP"
BASE COUNT         1910 a         2295 c         2122 g         1945 t
ORIGIN      
        1 gcgtaaacgc cttatccggc ctacatggcg ttgcaattta ttgaatctga atgatttttt
       61 aggccaaata aggtgcgcag caccgcatcc ggcaacgtta caccgactcc agccgggcgt
      121 aagctgccac cagccatttg attccctggc cctggaatgc cacctgcaaa cggctatgtt
      181 caccgctgcc ttccatattg acgatggtgc cttcaccaaa cttagcgtgg cgtacacgct
      241 ggccgagctt gtagccgctg tcgttctcga ccatcggcgt acccatccgc tgatggctga
      301 ccgggcggct taccgtggcg cgcaggcgca cctcttccac acactcttcc ggcaactcgc
      361 caataaagcg cgacgggcga tggtaaacct ctttgccata cagacggcga gtttccgcgt
      421 aggtcagcgt cagtttctgc atcgcgcggg ttacgccaac gtaggccaga cggcgttctt
      481 cttccagacg cccgccttca tccagcgaca tctggcttgg gaacatgccc tcttccatac
      541 cgacgataaa cacctgcggg aactccagac ctttcgccga gtgcatggtc atcagttgta
      601 ccgcatcctg ccaggtatcc gcctgccctt cgcctgcttc cagcgccgca tgggatagga
      661 acgcctgcag cggcattaaa tcttcgtctt cttcgttgta gctgaactgg cgcgttgccg
      721 tcaccagttc ctctaagttt tcgatacgcg tctgaccttt ttcgcccttc tcctgctcgt
      781 acatggtacg caggccggag tctttaatta cccggtcagt ctgtacatgc agcggcatat
      841 cggcagtttc ctgcgctaag gcgtcgatca gttccataaa ccgctgtaag gcgctggcag
      901 cacgtccggc gagggctttt tcctgcaaca gttcacgaca tgcctgccag agtgttaact
      961 ggcgatcgcg cgatgtctga cgtaccacgt ccagcgtccg gtcaccaata ccccgcgttg
     1021 gcgtattcac cacacgctca aaggccgcgt cgtcgttacg gttggcaatc aggcgcagat
     1081 acgagagcgc atctttgatt tcctggcgtt cgaagaagcg catcccgccg taaatacggt
     1141 acggcatact cgcctgcaac agtgcttctt ccagcacacg cgactgggcg ttactgcggt
     1201 agagaatggc gcactcggca agcgccccgc cgttgtcctg ccaggttttg atgcggttaa
     1261 ccacaaaacg cgcttcatcg agttcgttaa aagcgcaata gagggaaata ggctcaccgt
     1321 ccgcgccatc ggtccacagt tttttaccca gacgcccgtt attgttttca atcagggcgt
     1381 tagcggcgct cagaatattg ctggtagagc ggtagttttg ctccagacga ataatttcgg
     1441 caccggggaa atcattaagg aaacgctgaa tattctccac ctgcgccccg cgccagccgt
     1501 agattgactg gtcgtcatca ccgacgatca tcactttgcc ggtgtcgccc gccagcaggc
     1561 ggatccacgc gtactgaatg ttgttggtat cctggaattc gtccaccagg atattggtaa
     1621 aacgttcgcg gtagtgttgt aggatatgcg gcttgttaag ccacagttcg tgagcgcgca
     1681 gcagcagctc ggcgaagtcc accaggcccg cgcgatcgca cgcttcctga tacgcctgat
     1741 acaccttctg ccaggtctgc tccaccggat taccgtagct ttgaatatga tgcggacgca
     1801 ggccttcatc tttctggctg ttgatgtacc acattgcctg gcgcggcggc cactgcttct
     1861 cgtcgaggtt catggctttg atcagacgct taagcaggcg tagctggtct tcgctatcga
     1921 ggatctggaa atcctgcggc agattggcgt ccatatggtg cgcacgcagc aggcggtgcg
     1981 ccagcccgtg gaaggtgcct acccacatgc cgccctggct ggtgcccatc agttgtccga
     2041 tacgatgacg catctccgcc gccgctttgt tggtaaacgt cacagccata atcgagtatg
     2101 gcgagcagtt ttccacgctc atcagccagg cgatacgatg caccagtacg cgcgtcttac
     2161 cactgcccgc gcccgccagc accagaaggt tgctgcgtgg cgcggccacc gcttcgcgtt
     2221 gtttgtcatt aaggctgtcg agcaggtaag aaacgtccat tggcaccgcc gcgtaaaaat
     2281 aggttgggcg gagaagcgca acgccctccg ccaaaaagct gggtatatat acagatttgc
     2341 tgattatatc agcgaggtca gagatgccaa ccgggaaatt tccagatgtg gcagtaaacg
     2401 gctgtcccag gtttgcatca gatcgccatt ttccggtctg atccaacaag cctgcattcc
     2461 gctgcgaatt gccccaccca cgtcagtggt gagatcgtcc ccaacatgta agatctcgcc
     2521 gatcggcacg ttgagttttt ccgcagccaa aaagtacata tcgctgaacg gttttgagcg
     2581 cccgtgcggg ccagcgcgca gcacaaactc aaaataatcc cccaaaccaa acagttccgg
     2641 ctgggcgtta ccgttggtga tcgccaccag cggccatttc ttcgccagct gttttaaggt
     2701 gtcgtgagtt tgctgcggga cgtcgattcg gctgcgccat ttggcaaagt taatcattgc
     2761 tgcgtgtgcg cctgcactgg cttcttcggc actcagcccg gcgtcgagca tcgcttgttc
     2821 aatcgaacga aaacgccaac gtgtcacgtc gtgataaatc tcgggttccg cttcccgtac
     2881 cgcctggcgc aggcgttgca gatcttcatt ctggaagctg cgcagcgccg gatgataatt
     2941 ttgcacaaag gtaagcgcct ctcgctcggt gcgcaaaatc accggacggt tatcgtaaag
     3001 ggtatcatcc aggtcaaagg tgagcgccga gatgcgcccc aaaggccggt aaaaacgcat
     3061 tatttccccc gtttggcgcg tggatgcgcc gcatcgtaca ccgaggcaag gtgttgaaaa
     3121 tcaagatgag tatagatttg cgtggtggag aggttggcat gacccagtag ctcctgcaca
     3181 ccacgaagat cgccgctcga ctccagcata tgcgtagcga acgagtgacg taatttatgt
     3241 gggtgaacgt gattattcag cccttgtttt atgccccatt cggcaaagcg tttctgcaca
     3301 ttacgcgcgg agatacgctt gcccagtttc gacagaaaaa gcgcgtcgtc ttcgctacca
     3361 aacaggtcgc gcaaatcaag ccagtgctca atccacgcca cagcgttgcg accaatcggc
     3421 aggcggcgct ctttgctgcc tttccccatc acccacactt cgccggactc caggtcgagg
     3481 tgtttgatat ccagccccac cagctcggaa agacgcagac ccgcgccgta catcacttcc
     3541 agcattgcac ggtcgcgtac agcgagggga tcgttgatat caatatccag cagccgattc
     3601 atatcgtcga cgtcgatatt tttcggcaga tgacgcggcg ctttcggtgc cgaaactcct
     3661 ttcgccgggt tagctttgag ttcgttctgg ctgaccagcc agtcaaaaaa gctacgtagc
     3721 gcagaaagcc gtaacgccag acttgctgct cccagccctt tacggcgact gcgtacagca
     3781 aaattgcgta ccatcgctgc atcgcattgt tgccagcttt gcaggccgtt ttcgctggca
     3841 aaattgatga tcgcctcaag ctgacgctgg tagttaagta gggttatcgg gctaagctga
     3901 cgctccacgc tcagataacg taggtagcgt tctacatcgg tgtgtaaatc ggtcatacgc
     3961 gttcaatcca acgctccaga agctccggca acatcagcgc aatttcatga agtaactgcg
     4021 ttccctgccc ttgttgatag tgactggcat cgcgactggt aaacagcaca acacccaaat
     4081 cagcatcgct tcccagcatc gacatcgcca ccgatcccac cgctttcgct tccggtagca
     4141 ccaccagcag ctccggtccg ttaagcggcc caagatagtg ctgttcctgc cccaaacgct
     4201 gaatacgcag cggttcgaaa gactgacggc ttaatgccag atgagtgtgg ttcgacggcg
     4261 cacctaagcg ccagcgatct ggaaacaggc gcagactcgc accagctagg ccgagatcgc
     4321 gcgcccagcg gtgaaagcgc atcagcatat cgtcgagact gctggcggcg gtgagactgc
     4381 gctgcaggta gagtaggcga taaaacagcc cttcgttggc gatagcctgc tccatcaaca
     4441 acgccatgtt ctcttccaga acatgaatgt gattacgtgc gcgggccatg tgccactcga
     4501 ccaacgaaac ggtgccgcgt accggatgcg gcacacgtat cgcttctact gcgcgcgcat
     4561 tgcggataaa aaactcagga tttttaatca gataatcgac aaccgcccgg tcatcaagct
     4621 ccgtgagtgt ttcctgcagt tcttcccctg gttgcttcat agatgaataa atccatcgta
     4681 gacatgtacc gccgggccag tcatatataa cgggtgaccc ggacctttcc aggcgatatc
     4741 aagacgaccg ccggggagtt ccacgcgtac ttcttcgtcc agcaaacctt gctgaatccc
     4801 taccgccacc gccgcacacg cgccgctgcc gcaggcctgg gtttctcctg ccccacgctc
     4861 ataaacgcgt aaacgaatat gctcgcgctt aaccacttgc ataaaaccga tattggcgcg
     4921 ctccggaaaa cgctcgtgac tttccagaac aggaccaagc gtttctaccg ccgcggtatc
     4981 gacatcatcg acctgaatca cgcaatgcgg atttcccatc gacaccacgc cgcataagat
     5041 tgtctgctcg gcggcgcgca taatataggt cttttccgct ttgttagcgc gaaacggcac
     5101 ggcggaaggt tcgaagttgg gttcgcccat atttacgcgg accagatcat catcggtgac
     5161 ggtcagaacc atccgcccgt tggcggtgct gacgcggata tcacgcttat tggtcagtcc
     5221 tttcagacgc acaaaacggg caaagcagcg cgcaccgtta ccgcactgcg ccacttcact
     5281 gccatcagca ttgaaaatgc gatagtgaaa atccagttca ggatcatacg gcggctcaac
     5341 caccagcagc tggtcaaacc ctacccccag gtgccgatcc gccaggcgac gaatcagctc
     5401 cggtgaaaaa aagacattct gcgttaccgc gtcgacgacc ataaaatcgt tgccaaggcc
     5461 atgcattttc gagaactgca tcatttactc caatcacgcg ggtacagaaa ctgactttta
     5521 gtaattcacc tgggatggac catcgccagt ggcgcgatcg tttttatccg gcaccgtgga
     5581 ttgcgtttgc gtctctaccg gtttggtcgg cggcggtgcg tttttatctg caggcgggaa
     5641 atagagcgga cctttcagac cgcagcccgt caggctgaag agagtaagta atacagtgag
     5701 tgccttaaac acgtttttca ttatggattg cctgtaaagt tcatcgcttt ctgctttcta
     5761 tcatcgcagg agaaggcgta aaagcaagcg tttagcgact ctttatgaag tctgaaaagt
     5821 gaggaaatgc tgcgagtata acgcttactc gtgaggattt atgttctgct ggcagtttcg
     5881 tgatcaacac ctcgcgccag gatggcttta tgcactatcg caatgtggtt tgtttgttgt
     5941 cttgttcatt gtttttatcg tcggcatggg gatgtcggct ttccgcacct gaacataata
     6001 tttaccagaa acagggaaaa ggcgtggtgt acctgcgccc ttataaagaa acaaatcttt
     6061 ctctacctga cgtgaattac aagagtttgc gtcggttacc caatttatta atagacccga
     6121 caacactaga cgaatgggat aaagaaccgc ctctgactga tttgacaacc gactacctct
     6181 atgagggcgc acaggcctgg tatcctcatt attcatggca tagcgatggt cgcaatatcc
     6241 tctacgcggg tgaagttgtg caaaaccctc ccggcacacc tcccgtcgat gtcgcctcgt
     6301 tcaaggcatg gggtgatttt gccgcagata aacacagtct ctattttgaa ggcaagcgca
     6361 ccgatgacaa tggtggtgga aatagcttgg atatcaaaac gctgcatcag gtggaattcc
     6421 ggcctccctg ggatcctgac ttactggggc tgatattacg tgacgctaat ttcttgtata
     6481 tcaatgggca tcgcctcgct gaccccgaga gtttccgtgt gctggcgcag aagtcctggg
     6541 atcagcgagg aaagttttcc accacgttta acccctgcat tgccgtacca tttggcccct
     6601 gggataccct ggctcgtaca cggaccaaaa tcctgctcaa cggcgaacag cttgatgccg
     6661 acccggacac cttttccgtc gtgcgctgga tgcccggctc actcttgacc tggcgtgata
     6721 aaaacgggct gcagcgtaaa gtcctcgaca aggaaaatct ggcgtgggat gaagatttaa
     6781 caaagcactg tctggatttt tctctgctgg aaaagaaagt gttctggcgt aaaggacctg
     6841 cttgtaaaca ggaagaatta cccggactcg atccggagca gtttcaaccc atcagtgatg
     6901 ctgtcgccca gcatcaggac tcgctttata ccatcatcga aacagagtct ggtaaccgta
     6961 agctggagat cgtgaaactt gatgatccca atcttattat caacaaacgt ttcaacgccg
     7021 ggaaacgcca cggctattta cttacgcgtg ccgaagggta gccataccat tccggtttac
     7081 acgtgtttga atctgacgga ccgctgatct tactggataa ccgttctccg gatgaacgcg
     7141 aagcccatct taatgaccat ccctttttgc gcagatggta tgcccgcgat aaccgctaca
     7201 tttacagctt tgatggcgcg cagctctggc gataccgcac cgctgatccg aaacaagttc
     7261 gcttaatctg gaaggaacaa cattcgggat atgtctatgg cgtaaattac aaaacgggat
     7321 atctggacgg aaaaattact gatgacggcg aatttattcc tgccccgcgc aatgaggcga
     7381 caaaatgaag ggtcaaataa tcacagttgc actcatcctg ttgggacaca catttctgac
     7441 gcctgctgta caggccatag ggttcgacta ttacaatgat cacggtgtta tgtcttacgg
     7501 taaaggttat tggggggata agaaaatcat tgcccagttt ccggagatga gcagggccgg
     7561 tttgcgcctt gtgaaaaata tctctgggaa aacgggttat ataccttccg atgaaataag
     7621 catagaaaat gaggaatacc actgggtgac agacggccgc gttattctct ggcgtggcaa
     7681 aatcgttagc aatccaccgg gaacactcac tgtcgatatt gccagctttc aggctatggg
     7741 ccgcttcgcg gtcgataaat atagcctcta ttttgacgga cagcgcaccg aaagtaatag
     7801 cggcgcgtca cgtgttgagc tggcgacact aaaagctatc gaaggtaact ctaccacgct
     7861 ggtggatagt aaaaatctct acttgtccgg tcgacgtcag gggagcagca gtgatgttac
     7921 tgtattagaa aaaagatggt ggggtattaa tccacgtctt atgagtgtaa acagaaattc
     7981 atattccaat gatctgctta tccgcagtgg gcagaatatt tatttaaatg gcgttcacct
     8041 tacggcgaat gcagactcat ttgagataat tcgctggata cctcattcac tgctggtttt
     8101 tcgcgacaat aagggtctgc atcgttatcc ctttggtcaa ttatcaggca aagcgatacc
     8161 cgtagatgat gacgtctctt ttgaagtagg ggaaagtcgc gttcgctggc gtaaacagct
     8221 cacgcccgac cgtcagtgga gcaagtggat agacctacca ggtattgaac ct
//
LOCUS       DAJTRD010000146         8115 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTRD010000146 DAJTRD010000000
VERSION     DAJTRD010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.146"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..1332
                     /gene="yjeM"
                     /locus_tag="OO315_003748"
                     /pseudo
     CDS             1..1332
                     /gene="yjeM"
                     /locus_tag="OO315_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="OO315_003749"
     CDS             complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="OO315_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5892498.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4816..5784)
                     /gene="psd"
                     /locus_tag="OO315_003750"
     CDS             complement(4816..5784)
                     /gene="psd"
                     /locus_tag="OO315_003750"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5892499.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="OO315_003751"
     CDS             complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="OO315_003751"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5892500.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7028..7573
                     /gene="orn"
                     /locus_tag="OO315_003752"
     CDS             7028..7573
                     /gene="orn"
                     /locus_tag="OO315_003752"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5892501.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            7784..7859
                     /locus_tag="OO315_003753"
     tRNA            7784..7859
                     /locus_tag="OO315_003753"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7817..7819,aa:Gly,seq:gcc)
     gene            8017..8092
                     /locus_tag="OO315_003754"
     tRNA            8017..8092
                     /locus_tag="OO315_003754"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8050..8052,aa:Gly,seq:gcc)
BASE COUNT         1855 a         2093 c         2200 g         1967 t
ORIGIN      
        1 cttaatgatg gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta tctattcctg
       61 gatgaataat agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc
      121 ttatatcatc tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct
      181 ctatggtagt gacatgaccc agcactggcg tattgccgga ctggagccta cgcaggtggt
      241 tggtctgctg gcagtagcat ggatgattct ggtcacagtc gttgcttcaa aggggattaa
      301 taaaattgcc cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt
      361 gctgttagta agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa
      421 tttccttgca tcaccgaacc cgggttatca gtccggtctg gcaatgctat cgtttgtggt
      481 atttgctatt tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga
      541 aaatccagaa aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg
      601 ttattcgctg gcaatatttt tatggggcgt cagcacaaac tggcagcagg tattaagtaa
      661 tggttccgtt aaccttggca atattaccta tgtgctgatg aagagcctcg gggtgatgct
      721 gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat ggtttgcgcg
      781 tattaccgga ctttcgatgt tcctcgctta taccggtgcg tttgctattc accgctgaaa
      841 gccatcatcc aggggacgcc gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg
      901 atggggatgc cgtctatcgc catgtggatg cagtgcgggt tggttactgt cttcattctg
      961 ctggtttcgt ttggtggcgg taccgcatcg gcgttcttta acaagctgac gctgatggcg
     1021 aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg ctttcccgtt ttttaaagca
     1081 cgtcaggatc tcgacagacc atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg
     1141 acggtggttg tcgttctggt ggtgacattt gcgaacgtct tcaccattat tcagcctgtg
     1201 gttgaagccg gagactggga cagcacattg tggatgattg gcggccctgt cttcttctcg
     1261 ctgttagcga tggcgattta ccagaactat tgcagtcgca tggcgaataa acctgagtta
     1321 gctctcgact gatcatatga aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag
     1381 gaagtaatgc gtatggatga tactctccag ggccggtgat aactgaagat gatgttaagc
     1441 tgctaaatga gccacgttga catcatttgg cagcttcatg cattacaaac ttcccgcctg
     1501 acgacctttg cccgcagacg tcagcgttct ccccgtttgt ttaccgttga ggctttccag
     1561 acgcatctgg aagggcggga atggcatatc gataccgtgg gcgtggaagc ctgccagaat
     1621 cagctggtgg atctcatggc gtagcggcat acggtgaccc atctcagcgg cgtaaatacg
     1681 cagctcgaaa atctgaatcc cctgttgcag atccaccagg aagacttccg gtgccgggtt
     1741 gtcgatcacc agcgagcagc gacgcgctgc ggtgagcagg atttccgtca cttcttcact
     1801 gttggcatcg gcaggggccg gaatcgtcaa cactacgcgc gtgaccgagt cagagagcga
     1861 ccagttgata aactgctcgg taataaacgc cttgttcggc acgattatct ctttacggtc
     1921 ccagtcgctg atggtggtgg cgcgggtgtt aatttttgtc acgctaccag taagatcgcg
     1981 gatcgttacc gtatcgccaa tgcgaatcgg tttttcgaac aggatgatca ggccagagat
     2041 aaagttggcg aaaatttcct gcaaaccaaa accgagacca acaccgagcg cggcaaccag
     2101 ccactgcaat ttcgaccact caataccaat cattgagaag ccgaccagcc cgccaatcag
     2161 catcagcaga tattttgtga tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag
     2221 gtgctgcaaa atcgccagtt ccagcagcgc gggcaggttg cgcaccagct gcgtggtgat
     2281 gataaatacc agaatggcaa tcagcaccgc accgagggta attggctcca gactttctac
     2341 gccctgtacc gtggaggtga catcccacag cgaaatattt tcgaggaagc cgaaagcgga
     2401 atggatttct gaccacagca caatgaccga aagcagggcg atcaacatca aaattgagcg
     2461 caccagccgc aaggattgcg cactgatggc atcgagatcg acttcgcttt catcgacttc
     2521 aattgctcct tccgggctac tgtgatgatg cgcttcctct tcgccacgcg cacgttgcgc
     2581 taacatctct gcccggcgat gcttcgcccg gtcaaacgcc agcctgcgac gctggatcag
     2641 catccagcgg cggataacgt gataaaccac cagtagcagg aaccagatgg caaccgaggt
     2701 ttcaagcctc gctaacagtg cctgcgccgt tgccagataa cccaccgccg acgccagaat
     2761 ggcaaccagt ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa tgttgtcgcc
     2821 gctgccttct ttgttgagat acaacggaat accggctttt ttcaggctta gggtgaccac
     2881 cgccagcgca ccgcaaatga ggataaagca aagccgtccc agcgaaccgg aaaattcgcg
     2941 gtcgtcgagg ttatcgaaca tcatcagcgc cataatcagc ccgatgctca tcaggtagta
     3001 gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt
     3061 cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg tggccgttac
     3121 accatcgcca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc cgtaacccag
     3181 cgtcatccac agcaccggta acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa
     3241 cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa
     3301 acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaaacag
     3361 cggcaaaatt gtctctttgc tggtcagcat catcacactg gctttgccca actgactgaa
     3421 ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagcggcc aggcgatggt
     3481 catcgggcgc acgtcagagg cccagaacag atagcggtgc gttgcttcgt tcacctcttt
     3541 cagcgcatcc tccagttgcc tgttggagac tttcagcttg gtcagttcca atagtagcgt
     3601 gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag
     3661 aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatggattt gccgtagcag
     3721 cggctgttta ttaagtaaat cctcataacg taaccgttgc acacgcaact gcgccatttc
     3781 ggtatcaagc tgttgtggtt ttggcatttc tggtagccgt gccacctgcg cccgcagcgc
     3841 ttcgccgagc aggttggacg atcccagcca ttgcgactgt tcacgcagcg tattcaacgc
     3901 ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt tgcgaggcaa cgagctccat
     3961 ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg ttaattttga attgcgcgac
     4021 gatatctttc ggcaaatcgg cactgttttc tgccagtaat tcggtacttt ccagcgcccg
     4081 ctccgcctca agctgacgtt ggctattcaa ctgattacgt aaggcctgca aatacgcatc
     4141 cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt
     4201 attggcagac agctgcgcca gctccagttc atcaacgagc gccttaagac gtgcagagtc
     4261 agactgcaac gcgaaatttt gtgcctgatt gagcggagta ttgccggtaa gcgttcccag
     4321 gcggcgctcg atctcattta actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt
     4381 cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc
     4441 cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acattcccgg
     4501 cgacacgctg cgcggctcgt cacgaatgtt gtttaattgt gcgcgcagag tagcggagag
     4561 tttcggataa ttatcgataa cttgctgata ttgtttgatg cgctcaaggg aaccttttcg
     4621 ttcctcaagc gcatttaagg cagactggag cgcctctacg acttccggct gtgcgggttt
     4681 cgccgctttt gcctgctcca gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc
     4741 gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc
     4801 gtttcctttg atggattaga cctggtcttt tttatcgtca accaatgggc tggcgtcgtg
     4861 ttctgcttcg atctcttcag caggaagtgg ggcgggttca gcgtctggcg taacaaaggt
     4921 ttcggtagat actgccagcg gctggccaat tttcgtgaca gacaggcttt ccagttgctc
     4981 aaccagattc actttacccg gtgcaaacag gttgataacg gtggaaccga gtttaaagcg
     5041 acccatttcc tggcctttca gcagtgccac agaaccgtcg ttttccccgg caggccaggt
     5101 ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat
     5161 gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa
     5221 aaggcaaatc acgcgttcgt tacgggcaaa cagatttggc acgttctgag cggtgagatg
     5281 gttaacggaa aagagatcgc ccggcacgta gatcatctca cgcagaatac cgttgcacgg
     5341 catgtgtacg cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg
     5401 gaacaggtcc gccatcagat agttgcctgc cagcagggct tcgaggctgt agttgtggcc
     5461 tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc
     5521 aggcatgacc agtacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg
     5581 gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat
     5641 gtcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg
     5701 cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag
     5761 cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc
     5821 cggccaacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg
     5881 tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat
     5941 acggtgatag ttttcgaaac gggtttccgc gattttccct tcctcaaccg cttcccggat
     6001 agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaaatagtc
     6061 atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc
     6121 acgcacacca ggggaatcaa tcacatcacc gccgtgcggg aagtgataca gccgagcggc
     6181 ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca tcgttggtca ggatctcttt
     6241 ttgcagcccc agcaacgcgt tcagcaggct ggatttaccg acgccagact gcccggcaaa
     6301 aatgctgatg cgcccggtca acgcctcttc cagcggtttc agcccatcct gagtatggct
     6361 ggaaaccatc aatacgcgat aaccgatgtt gcggtagata tccatctgct cgttgacgaa
     6421 cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc acgataatcg gctcaatctg
     6481 caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat
     6541 ggcggagaca atgacaatct ggtcgatgtt ggcggctatg ggtttcacgc cgtcgtagaa
     6601 atccggacgc gttaacaccg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt
     6661 tacgccttcc gccgccggtt tacccggacg ccagactacg cggtcgccgg ttaccagcga
     6721 acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc
     6781 gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc
     6841 gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg
     6901 ctgctggcct ttggagagtt tatttttact caatcgtact gactcctggt cgcccctgat
     6961 gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga
     7021 aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct
     7081 ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaatctgaa
     7141 tattctggca gaagggccga ccattgcagt acatcagtcc gatgaacagc tggcgctgat
     7201 ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtagagcgcg tgaaagcgag
     7261 cacgatgggc gatcgggaag ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc
     7321 tgcgggaaaa tcgccgattt gcggtaacag catcggacag gaccgtcgtt tcctgtttaa
     7381 atacatgccg gagctggaag tctacttcca ctaccgttat ctcgatgtca gcaccctgaa
     7441 agagctggcg cgccgctgga agccggaaat tctggatggt tttaccaagc aggggacgca
     7501 tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg gcttactacc gcgagcattt
     7561 tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg
     7621 attttgacgc taaatcgaac gtcttgctga taatttcagc acttgagata aaaacgcaaa
     7681 aaaaactttt ttggggggtt gcagagggaa agatttctcg tataatgcgc ctcccgtaac
     7741 gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag tacgcgggaa tagctcagtt
     7801 ggtagagcac gaccttgcca aggtcggggt cgcgagttcg agtctcgttt cccgctccaa
     7861 aatttgaaaa gtgctgcaaa gcacagacca cccaatcgca gtatttcgaa ttgcgacaag
     7921 gcggcaactg aacgaatcct tgggaactta caaaagtatg tgactaaggt gagtgaaggc
     7981 agccaaggca gtagcggttc gaaagacgaa gattaagcgg gaatagctca gttggtagag
     8041 cacgaccttg ccaaggtcgg ggtcgcgagt tcgagtctcg tttcccgctc caaaatttaa
     8101 aagtgctgta aggca
//
LOCUS       DAJTRD010000147         7889 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTRD010000147 DAJTRD010000000
VERSION     DAJTRD010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.147"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..647
                     /locus_tag="OO315_003755"
     CDS             <1..647
                     /locus_tag="OO315_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892502.1"
                     /translation="RIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQEE
                     LCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEAAK
                     RNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPMTF
                     SRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            619..1053
                     /locus_tag="OO315_003756"
     CDS             619..1053
                     /locus_tag="OO315_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5892503.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1826..2875)
                     /locus_tag="OO315_003757"
     CDS             complement(1826..2875)
                     /locus_tag="OO315_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5892504.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINAINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3326..4516
                     /locus_tag="OO315_003758"
     CDS             3326..4516
                     /locus_tag="OO315_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR5892505.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4906..6366)
                     /gene="gtrII"
                     /locus_tag="OO315_003759"
     CDS             complement(4906..6366)
                     /gene="gtrII"
                     /locus_tag="OO315_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR5892506.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6363..7292)
                     /locus_tag="OO315_003760"
     CDS             complement(6363..7292)
                     /locus_tag="OO315_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5892507.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7289..7651)
                     /locus_tag="OO315_003761"
     CDS             complement(7289..7651)
                     /locus_tag="OO315_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5892508.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT         2452 a         1492 c         1597 g         2348 t
ORIGIN      
        1 atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag aacggtttta
       61 cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac tactttgaca
      121 tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg gagaaggggc
      181 ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag aatccgtttg
      241 gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt ttgggagaag
      301 cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc ctgttcgcgt
      361 caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt ttacagtgcg
      421 gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc ccgatgacgt
      481 tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct gggcagccga
      541 ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg cccggttcag
      601 gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa aatgtcatat
      661 ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac caatattcct
      721 gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc acagagtgta
      781 acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg tccttctcca
      841 acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat agcagaggca
      901 acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac agatgatgaa
      961 acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt taatgtagta
     1021 aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat aattgttgga
     1081 ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact tctacaatga
     1141 tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa acaatatgac
     1201 tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag cccttgctgg
     1261 gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg agttttgttg
     1321 cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta ggtgcagcta
     1381 tttagactat atcgacgcag taacgataat agatatcgaa actgtactag atattagcta
     1441 cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct tcaaataatt
     1501 cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag ccagtttatc
     1561 agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata tgatatttta
     1621 attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt cagtgaaggg
     1681 gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa aaccgtaaca
     1741 tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat atctataaaa
     1801 aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg actgtcctcg
     1861 atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat atgattgcgc
     1921 agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata gggataaata
     1981 atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct cttacaataa
     2041 agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag cctaccgtgg
     2101 tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca ttgaatattg
     2161 agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc attatagcgg
     2221 taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca cgtgttaatt
     2281 ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg gagtctatgt
     2341 tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt gacaacatga
     2401 acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct ctaaagattg
     2461 catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct acgaattggt
     2521 tatttctaac accatatatt atatcataaa tgtggttgta tatataatca atcgtctcac
     2581 ctgagttaat tgcgtttatt attaatatag aaaatgcaaa agtgaaatat ggaatcatta
     2641 atctgtaaaa ctttgacttg atataatcga aaacagataa gtcatttttt ttgatagtaa
     2701 aaaaaccagc agcgaaaaag aatagtggga cgtgataact aaaaacaaaa ggatacaatt
     2761 tgccagcccc caacccaagg tgacctaaat atatagcaaa aataccgaga aattttaaag
     2821 catcaaccca atcatgtctt atttttctgt tgctgtcgat tttgttatta tacatttaaa
     2881 gtaggctctc cagttttgtg tgatttttaa acactcattg caaatatgaa tgcctgaaag
     2941 tttatgatta ttaatcaacg agttcaatat taatttatta tttttcaaag tcgcactata
     3001 taggcattta cttccgcagt accaccgata gtatcgatat caaatcagcc tgcggccgcg
     3061 ataactttcg taacattctg ccagcggctt tgttgaataa atcaggttca agataatcgt
     3121 ctctgcatca ggttagagtt tcaggcaata catcctcttt ctggcatacc tgccactctc
     3181 gcaccatggt catagccttt acgatctgca tactcaccag accagagccc gctccctttt
     3241 aggctgagga atccccagaa aagaagacag gcatagagtt ttgtgatcta ctgattgcgc
     3301 taacaaattc aatgagatca cctctatgcc tgcccgtaaa gtatgccaga cttttttccg
     3361 taatgcttta gcttccacgc atcagtatcg acaaaatgcc attattgatt ctgctgctgc
     3421 tttagtcggt ggcgcatctc tttctcttac cagcattggc cggcatttac ccggtcctgc
     3481 tcgcgtgaaa gataaaataa aacgcgttga ctgtctgttg ggtaataaac gacttcataa
     3541 tgatatccct ctgatattca aaaatattac atccatgatg acaaataaac tgtcgtggtg
     3601 tgttatcgct gttgactgga gcggttaccc ttttcaggaa tatcatgtcc ttcgtgccag
     3661 cctcctctgc gacggtcgct ctatccccct gatgagtcag gtcttcccgt caaaaaaaaa
     3721 taacgaggcg gtggaaatcg cttttctgga tgcgctttcc ggggccattt ctccccggac
     3781 tcgtgttgtt attgttactg atgctggttt tcaaagtgca tggttccgtc atatcaaatc
     3841 acagggatgg gattttattg gtcgtatcag gggggtagtg aagttcaggc tggatagtga
     3901 caaagataag tggctggaca taaaaatgtg cagggggagt tcagaggcaa aatacctggg
     3961 gacgggaacc ctggcccgga aaaaacgctc gcagtgtgaa ggacattttt atctttataa
     4021 acattcacct aaaggcagaa aaagcaggcg tgccagaggg aggccgggct taccaacaac
     4081 ggaaaaagaa caaaaagccg caggcagaga accatggtta atattcagca atacagctga
     4141 gtttaatgcc aaaaaaatca tgaaactgta cagtcgaaga atgcagatag agcaaaactt
     4201 tcgggatgaa aaaagtgaac gctttggttt tggccttcgg gcgagcagaa gtcggagggg
     4261 agagcgtttt ctggtattaa gtctactggt tacactggcc agtatcgtac tctggctact
     4321 tggttatcat tttgaaaata aaggatttca tttaaagtat caggctaata gtctcaaaaa
     4381 acgaagagta ttgtcttttc tgacgctggc tgaaaacgtc ctgagattta accctgaact
     4441 tctacgacga gcacaacctg aaaagatatt gtgccggttg gccagcactt accgaagtat
     4501 ggtgctggct tactgatgaa aattttctgg ggatccctca gcctttcggg gaggaatgag
     4561 ggctctgata ttcttttgcc gtagttcatc gtgaaaccgt cggatgtcat ttggcctgag
     4621 tgacccatgc ctcccaaaag cagagtcgct agtgttatgt aattaggcgg ttaaacttca
     4681 attctcagtg ttaagtttgg gtaaacgctt gtaaatggta ttgctaattt tttaattgac
     4741 taatattaga aacggttagt gtgattgtat atagatattt gagtgtaagg tatttaaagg
     4801 ggcattcagc ccctccgtct atttgtgatt gcatccattg atgtgtgtat tattttgagc
     4861 ttgaaaaagg gaggcgctct ttaataataa ataaatctat caaagctaaa tattaaatgg
     4921 aagccaccct tttttctctg atagttttat tatcaccaaa ccatttattt cagctatcga
     4981 atctggattc ggccaggaag gtaaactctt cacaattgga taaatcttct ctatttgttc
     5041 tttatttgct ggtttgcaat tacagattcc atagtaattc ataaatgcaa ccatcctaaa
     5101 atagttgcca ccatcccacc aaaaaaaaga ttttccaaaa gtgtctgcgc tcatggagag
     5161 catatttcct gagtcgtttg aaccatatat atatatatat tttccgttaa gggaaatgcc
     5221 ttttgtttga gatgtgtatg atatttcttt agctaaagag atatctttct gtcttgcgag
     5281 agtatcattc aaaaatagat ttttggatgt tgaaacgcca ttaaatataa taaataaaaa
     5341 aaacatgcag tttaggtatt ttatagagcg aaaattgctt aagcaagaaa acaaaattac
     5401 tgctactata ggcattaaaa caaacaggcg aggtggcgcc cctgagccaa caacaacgat
     5461 aaatataacc ggaagtatat aaatgaaaat gattgatgaa atcaaataaa ttgatcttgt
     5521 ttttaattta tatgttaggg taaacatcag aattaataaa ggaatcagta accacttgta
     5581 taggtttaaa ccagtgggag gattgttata gaagttatat atattatcta tggctgattt
     5641 aacaagccat ttaatatcgc ttgcattttg tatataattt gagatgtact cgttcgattc
     5701 aagcgaaaaa taatgcttga tagctttggt taatagcagg taaattaagg tagataaagc
     5761 tatgagggat agagataaac atgaactata aaaagattca cgaatatttt tctcatttcg
     5821 tattatcgaa tttatctgca acccaatgac gaatgcaata atatatgtta cgaatgtttg
     5881 gtagattgcc attgaaagaa tggacagtac tataccagaa aatatcacaa tcctattttt
     5941 ttgcgagtga aaaataattg ctgatatcgc tgccagtaga aaagcaattc ccacagtatc
     6001 agcttggtta agaaactcta attgatagga gatctgaggg aaggtaataa atactaacat
     6061 acctatcaat aattcataag attctagctt tagcgatctg cagattataa atgctgaaat
     6121 gatgataaaa gataaggcta ttaatggtgt tatataaagt gaaaaaggct cagggagaaa
     6181 gtaatgtcgc aaaaaagtgt gaaaccaccg cccaagcgtg attgtttgat aaaaattatt
     6241 tgtaaactcg ccatcaatat taagtgttcc atcgtacaac cacccccaat aacaataaat
     6301 tgatattgca aaacatgata tgaaggcaag gagatttgca tttttaaata aattaatttt
     6361 aatcatgggt tacctcgatg agattttttt atgatgtatt tgggtctatt ttttacttca
     6421 atatagattc tacctatata ttctccgaga acaccaatcc cgatcagttg cactccaccc
     6481 aagaaaagta ttgatacaag cagggaggga tacccgcgta ctgggttacc aaagacaagg
     6541 gtgtctataa tcatccatgc accatataaa aatgaaatgc ttgcaacaaa caagcctata
     6601 taagtccata cgcgaagagg aaaggttgaa aaacttgtga taccttccag tgccagattc
     6661 cataatttcc agccattaaa ttttgagatg ccagcaacac gctctgcgcg tacatattca
     6721 acgacatccg tcggaccacc cacccagctc agtatgcctt tcatgaaaag attgcgctca
     6781 ggcaacagtt taatgttctc cacaacctca cgagacatga gtcgaaaatc tccgacattt
     6841 tcctcgatct ttggggtgct tattttgttg tgtaatttat agaaccactc agctgtctta
     6901 cgtttcagtc gtccatcagt tgagcggtct gaacgtttag caagcaccat gtcagcacct
     6961 gcctgccact tttcaataag atgagggata acctcaattg ggtcttgcag gtcgacatca
     7021 ataggaatta cagcatcgcc gcttgcatgg tctaaccctg caaataaggc tggttcttta
     7081 ccaaagttgc gtgtaaatga cagcggaaca actagcgggt ctgaaacagc cagcgcgtta
     7141 ataattgact ctgtggcatc tttacttccg tcatttatga atacaatttc tacttcatat
     7201 ggcttcaact cttggaattc acgtaccgtt ttatagaaaa caggtatcgc ttcttcttca
     7261 ttgaagacag gaacgacaag agatattttc atttcgcatc cctaaagaca atgaactttg
     7321 aatagacgaa accgcacacc agactgatgg cggagaaggt gataagagtg actattggag
     7381 gaagtgagca tttatcagct gcccaaccaa caatcacact caatattccc ataaatccca
     7441 cgtataacat gtagcgcatc gctgtagtca atgctttgaa tgtgaatctt gcattcgcga
     7501 agaagctaaa gctcacagcc actacgaaac ctgtgaagtt tgccagagcc tgactggtat
     7561 gtgcggcata gatacataca ccaaaaacca cccagtgtat aagtgtgttc agcacaccta
     7621 tagatgtgta ctttacaaat aactttaaca tttatttaat caatgagctc tgaaaggcat
     7681 gaagtctatc atccaagtct caattgatcg atacttgcta tgtctgatga gacaaaactg
     7741 agacacataa ggcctcacaa tggcttgcaa ggctttacat gttttgatgt ggtgggacgt
     7801 gtgagcgcag tgttgatggg ataatccttt gaattacaag cggattctta taattcgtaa
     7861 tgcgaaggtc gtaggttcga ctcctatta
//
LOCUS       DAJTRD010000148         7721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTRD010000148 DAJTRD010000000
VERSION     DAJTRD010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.148"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..1003
                     /gene="ydfJ"
                     /locus_tag="OO315_003762"
     CDS             <1..1003
                     /gene="ydfJ"
                     /locus_tag="OO315_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5892509.1"
                     /translation="TLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMGTNCG
                     TLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEKVNDS
                     NQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAIPTDA
                     LMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPSTIMV
                     ALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAGIFCT
                     MTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPSRSL"
     gene            1092..2552
                     /locus_tag="OO315_003763"
     CDS             1092..2552
                     /locus_tag="OO315_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5892510.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2588..2791)
                     /gene="ydfZ"
                     /locus_tag="OO315_003764"
     CDS             complement(2588..2791)
                     /gene="ydfZ"
                     /locus_tag="OO315_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5892511.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2969..3655)
                     /gene="ydfH"
                     /locus_tag="OO315_003765"
     CDS             complement(2969..3655)
                     /gene="ydfH"
                     /locus_tag="OO315_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5892512.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3744..4490)
                     /gene="ydfG"
                     /locus_tag="OO315_003766"
     CDS             complement(3744..4490)
                     /gene="ydfG"
                     /locus_tag="OO315_003766"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5892513.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4627..6672
                     /gene="dcp"
                     /locus_tag="OO315_003767"
     CDS             4627..6672
                     /gene="dcp"
                     /locus_tag="OO315_003767"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5892514.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6717..7235)
                     /locus_tag="OO315_003768"
     CDS             complement(6717..7235)
                     /locus_tag="OO315_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5892515.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7511..>7721
                     /gene="ydeI"
                     /locus_tag="OO315_003769"
     CDS             7511..>7721
                     /gene="ydeI"
                     /locus_tag="OO315_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR5892516.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNL"
BASE COUNT         1936 a         1807 c         1952 g         2026 t
ORIGIN      
        1 gacgctgcgt attattcagg gattgggtgc aggtgcggaa atttccggtg ccggtacgat
       61 gctggcggaa tatgcgccaa aaggtaagcg cggaattatc tcctcatttg tggctatggg
      121 aactaactgc ggcaccttaa gcgcaacggc aatctgggcc tttatgttct tcattctcag
      181 taaagaggaa ctgctggcgt ggggatggcg tataccgttc ctggcgagcg ttgtcgtgat
      241 ggtctttgct atctggttgc gtatgaatct gaaagaaagc ccggtctttg agaaggttaa
      301 cgacagcaac caaccgacag caaaacctgc acctgctggt agcatgttcc agagcaaatc
      361 cttctggctg gcaacagggc tgcgttttgg tcaggcgggt aactccggtt taattcagac
      421 tttccttgca ggctatttag tacagacgtt attgtttaac aaagcaattc caacagatgc
      481 attgatgatc agttcgattc tcggctttat gaccattccg ttccttggtt ggttatccga
      541 taaaattggt cgccggatcc cgtatattat tatgaatacc tctgcgattg tgctggcgtg
      601 gccaatgctt tctatcatcg tagataaaag ctatgccccg agcaccatta tggttgcact
      661 gattgtgatt cataactgtg cggtgctggg attatttgct ctggaaaaca ttaccatggc
      721 agaaatgttc ggctgtaaaa accgcttcac ccggatggct atttcaaaag aaattggtgg
      781 tcttatcgct tccggttttg gtcctatcct ggcgggtatt ttctgcacca tgacggaatc
      841 ctggtatccg atcgccatta tgatcatggc atattcagtg attggtttaa tctctgcgct
      901 gaaaatgcca gaggtgaaag accgtgattt aagtgcgctg gaagacgctg cggaagatca
      961 accgcgtgtt gtaagagctg cgcaaccttc cagaagtctg taaaccctca atcccttctc
     1021 ttaccggaga ggggattttt attcatataa caaaacatat agcttgccat atttaaattt
     1081 aaggaattgt tatgggaaat aatttgttat cagcaaaagc gacactccct gtttatgatc
     1141 gtaataacct ggccccaaga attgttcatt taggctttgg tgcatttcac cgtgcgcatc
     1201 agggcgtgta tgccgatatt cttgctacag aacatttcag tgactgggga tattatgagg
     1261 tcaacttaat cggcggcgaa cagcaaattg ccgatttaca acagcaagat aatctttata
     1321 ccgttgcgga aatgtcggcc gatgcgtgga cggctcgcgt cgttggcgtc gttaaaaaag
     1381 ccttgcacgt acagatggat ggcttagaaa ccgtgttggc tgcgatgtgt gaaccgcaaa
     1441 tcgcgattgt ctctctgaca atcaccgaaa aagggtattt ccactctccg gcgaccggac
     1501 agttaatgct cgagcacccg atggtcgctg ccgacgtgca aaatccccac cagccgaaaa
     1561 cagcaacagg ggtgattgtt gaggcgctgg ctcgccgtaa agcggcagga cttcccgcat
     1621 ttaccgtcat gtcatgtgac aacatgccag aaaacggtca tgttatgcgt gacgttgtca
     1681 cttcctacgc gcaagccgtt gatgtaaaac tggcacaatg gatcgaagat aacgtgactt
     1741 tcccatcaac aatggtggac cgtattgtgc ccgcagtgac agaggatacg ctggcgaaaa
     1801 tcgaacaact taccggtgtg cgcgatcctg ctggcgttgc ctgtgaacct ttccgccagt
     1861 gggttatcga agataacttt gtagccggac gtccggaatg ggaaaaagcg ggagccgaac
     1921 tggttagtga tgtgctgcct tatgaagaga tgaagttgcg catgctcaac ggcagtcatt
     1981 cattcctggc atatctgggt tatcttgctg gatatcagca cattaatgac tgtatggaag
     2041 atgaacatta tcgtcatgcg gcgtatggca tgatgttgca ggaacaagcg ccgacgctga
     2101 aagtgcaggg cgttgatttg caagattacg ctaaccgatt aattgcacgc tatagcaacc
     2161 cggcgttacg tcatcgaacc tggcagattg cgatggatgg cagccagaaa ttgccacagc
     2221 ggatgttgga ttctgttcgc tggcatctgg cgcatgacag caagttcgat ctgctggcgc
     2281 tgggcgtcgc gggttggatg cgttatgtcg gtggtgttga tgaacaggga aatccgatag
     2341 aaatcagtga cccactgtta cctgttattc agaaggctgt acaaagtagt gccgaaggga
     2401 aagcgcgcgt ccagtcattg ctggcgatta aggcaatctt tggtgatgat ttgccagaca
     2461 atagcttgtt tactgcaaaa gtgacggaag cgtacttgtc tttattagcg catggcgcga
     2521 aagcgaccgt ggcgaaatat tccgtgaagt aaaaaaagca atgccgccat cggcggcatt
     2581 cacacactta gttcatgccg agacgaatca ggtccagagg tgccagtttc tcttcacaac
     2641 cttcaacaac tacggttttt ccgcggcgga tttgctcagc ggtcagacct tcagtatcaa
     2701 tcgacaggat cttgccagtg tgaccggtgc cgctaaccat aacacggctg ccagtggtga
     2761 ttgcgttacg gttacgatcg taggtcgtca taggtgtttt ctcctttctg atttacagtc
     2821 gaagcccacg taacgggcgc aatataaata ctcctgtcga gtgaaatatt ttttgttttt
     2881 gatcaagctc acatcttttt tgatgcatgc atgagccgta aggcgggggg aaaagcctct
     2941 catcccggat gggatgagag taaatgaatt actcttcgct aaaccagtcg ctgttttcct
     3001 ggcggatctg gcgcacggat tcgctgattt cctgcaaatg ttgcgtcatt gcacgttcta
     3061 ccgcatcgcc atcacgtttt tgcagggcag agaaaatatc aagatgctgg cgtaacagca
     3121 tttctggtgg agaaacatgg tcgaaactca tatagcgcac gcgatcaacg gtcgctttca
     3181 ggttctcaat ggtatcccac gccagttgac agtcggcaat ctgcgtcagg agttgatgga
     3241 agttgtcatc aagttcaaaa aaatcatcca gttgcttgcg ctcaatggca atgcgttgct
     3301 ggtgaagatt ttgttccagt tggtagcact ggctttcggt aatcatgctc gccgcccgac
     3361 gcgccaccgc gcactcaatg gcctgacgga taaaactgcc gttgcgcacc tgggccatgg
     3421 aaattttgtt gacgtagctg ccacgctgcg gacgaatttg aatcaggccg ttttccgcca
     3481 gtttaataaa ggcttcacga accggctggc gtgacacatt gaaacgaaca gaaacttctt
     3541 tttccgacaa cggtgtgcct ggtgcaatca ggcaatggac aatgtcgcga cgaagaatac
     3601 gataaatctg ctgattgaca ggctgtgtgg gattaagttg cgtttcgacg gtcattcgtt
     3661 cttgcttgtg agtgagttaa ctgcatgagt ctaccacttt tttgtgacaa gcaataaccc
     3721 ggcagttacg ccgggtataa aaattactga cggtggacat tcagtccggc atagctttgg
     3781 gtaaccggca tcatttccag ggtattgata ttgacgtgag caggcagcgt tgacacccac
     3841 cagacggctt cgctgacatc ttctggcgtc aatgcaacgg tattttgata ggttttttcc
     3901 gctttaccgt catcgccttt aaagcggaca ttggaaaact cggtgccacc caccagaccc
     3961 ggttcgatgt cggtgacgcg caccgccgta ccatgcagat ccgtacgcag attcaggcta
     4021 aactgacgaa caaacgcttt cgtcgcaccg taaacgttac caccggcata cggccagctg
     4081 cctgccgttg agccaatgtt aataatatga ccatgattac gttcaaccat acccggtaag
     4141 acggcgcgcg tcatatatac taggcctttg ttgttggtat caatcatcgt ttcccagtct
     4201 tcaacgctgg ctttatgcgc aggctccatg cccaacgcca ggccggcatt atttaccagg
     4261 atatcaatat tgctccactc ggcagggagc gatgccagca tctcttcaat agcggcgcgg
     4321 ttgcgaacgt ccagttgggc gatatacaga ttatctccca gttcgtctgt taactcctgc
     4381 aaccgctcct ggcgacggcc agtggcgata actttatgcc cttgttgaat aaaacgacga
     4441 gtaatgcatt caccaaaacc tgccgttgct ccagttacta aaacgatcat ctcactgttc
     4501 ctcaacgctt ttgtgtgtca ataacatagc acgtgatgct taagagcggg taatactggt
     4561 ttgcaagatt gcactccgtt atcagtaagc ctacgctatg gaacacctcg cgttcaggag
     4621 aagaaaatga caacaatgaa tcctttcctt gtgcaaagca cactgccgta tctggctcct
     4681 cattttgatc aaattgccaa tcatcactat cgcccggcat tcgatgaggg aatgcaacaa
     4741 aagcgggcag aaattgctgc catcgcgctt aacccgcaaa cgcctgattt caacaatact
     4801 attctggcac tggaacaaag cggagaatta attacccgcg ttaccagcgt cttttttgcg
     4861 atgactgcgg cgcataccaa tgatgaatta cagcgtcttg atgaacagtt ttccgctgaa
     4921 ctggcggaac tggctaatga tatctatctg aacggtgaat tattcgcgcg ggtagatgct
     4981 gtctggcagc gccgtgaatc cctggggctt gatagtgaat ccatccgcct ggtggaggtt
     5041 attcatcaac gttttgtcct tgccggagcc aaacttgcac aagctgataa agcaaaatta
     5101 aaagtactga atacagaagc tgcgaccctg accagccagt ttaaccagcg gttacttgca
     5161 gcaaataaat ccggcggtct ggttgtgaac gatatcgcgc agctggcagg aatgagtgag
     5221 caagagattg cgctggcggc agaggcggct cgcgagaaag gtctggataa caaatggctg
     5281 attccgctgc tgaataccac ccaacaaccg gcgcttgccg aaatgcgcga tcgtgcgacg
     5341 cgtgaaaaac tgtttattgc gggctggacg cgagcggaaa aaaatgatgc caatgatacc
     5401 cgcgctatca ttcaacgtct ggtagagatt cgcgcacagc aggcgaaact gcttggtttt
     5461 cctcattatg ccgcatggaa aatcgccgat cagatggcaa aaacgccaga agcagcactc
     5521 aactttatgc gggaaattgt tccagcggcg cgtcaacgtg cgagcgatga attagcctcc
     5581 atacaggcgg ttatcgataa gcagcagggc gggtttagcg cgcagccgtg ggactgggca
     5641 ttttatgcgg aacaggtacg gcgggagaaa tttgatcttg atgaggcgca gctcaagcca
     5701 tattttgaat taaacacggt gttaaatgaa ggtgtattct ggaccgcgaa tcagctcttc
     5761 ggtattaagt ttgtcgaacg ttttgatatt ccggtctacc atcctgacgt tcgtgtgtgg
     5821 gaaatttttg atcataatgg cgtgggactg gcgttatttt acggtgattt cttcgcccgt
     5881 gattcaaaaa gcggcggtgc atggatgggc aattttgttg agcaatcaac gcttaatgaa
     5941 acgcatccgg taatttataa cgtctgcaat tatcagaaac ccgctgccgg tgagcctgcg
     6001 ttgttactct gggatgatgt aataacctta ttccatgaat ttggtcatac gctgcacggc
     6061 ctttttgccc gccagcgtta tgccacgctt tccggcacca acacgccgcg tgattttgtc
     6121 gaatttccgt cgcaaatcaa cgaacactgg gcaacgcatc cgcaggtatt cgctcgctac
     6181 gcccggcatt atcagagcgg ggcagcaatg cctgacgaac tgcaacagaa aatgcgtaat
     6241 gccagcctgt tcaacaaagg gtatgagatg agcgaactgc ttagcgccgc acttctcgat
     6301 atgcgctggc attgcctgga agaaaacgaa gcaatgcagg atgtcgatga ttttgaattg
     6361 cgggcgctgg tggcggaaaa tatggatctt cctgctatac cgccacgcta tcgcagcagt
     6421 tatttcgccc atatttttgg tggcggatat gctgcaggtt attacgctta tctgtggacg
     6481 caaatgttgg ccgatgatgg ttaccagtgg tttgttgagc agggcggatt aacgcgtgaa
     6541 aatgggcagc gttttcgcga ggcgatcctt tccagaggta acagcgaaga tctggaacgc
     6601 ctgcatcgac aatggcgtgg taaggctcct cagattatgc cgatgctgca acatcgtggc
     6661 ttgaacatat aaacaccacc aatggctggc ctggagatat cgccaggcca gcctggttat
     6721 agcagtaatt gcagcagctg ggcgagggca aacctcaccg ctaaagctaa taccgtttcg
     6781 cagtcgccag caaaatgcat aaccgcagtg tagttctggc ctttaatatg ccacgcgaac
     6841 cagacggtac ccgctggcgt accatcttca ccgccctccg gtccgccgta gccggtaatg
     6901 gcaatactga catcagcatc cgcacgctct atggcacctg ttgccatttc tgctgccact
     6961 ttctcactca ccgcagaata tcgttcaaga gactgctggc ttacgctgag gattttcatc
     7021 tttgcctgat cggtgaaagt aacaaagcct gcaccgtaaa atttgggtgt atcttcagct
     7081 gcacacaggg cgctagccaa cttaccgccg gtgcatgatt cggcggtagt taagcgtaat
     7141 tgatccgcga taagtctttg actcaacgcg gatgtcaggt tttcaatagt gtcggtatct
     7201 gcgagttgta caattttatc tttattaatg ttcactgttt tttgtccacg ctattatatg
     7261 taaaggtgat ttgctttttc tgtatgctcg tccaatataa taattttcaa acatataaat
     7321 ccgattattc tgaaaacatg tttaagactc ttcaatcttc ttaactttag tgagtcaaat
     7381 tctctgaata tttgacagct agtgggctga ttttaaatgc cttataacaa ttggcagtat
     7441 tcttatttgg caattataaa aatagcaacc agacttaata agtcaaacca aacgcaatta
     7501 aggagttagt atgaaatttc agtccattgt attagcaagt tttcttgtca tgccttatgc
     7561 gttggcagat gaccagggcg gtttaaaaca ggatgcagcg ccaccgccgc ctcatgcaat
     7621 agaagatggc tatcgcggaa ccgatgatgc aaaaaaaatg accgttgatt tcgcaaaaaa
     7681 catgcacgat ggcgcttcgg tttcactacg cggtaatttg a
//
LOCUS       DAJTRD010000149         7603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTRD010000149 DAJTRD010000000
VERSION     DAJTRD010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.149"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(52..531)
                     /gene="xdhC"
                     /locus_tag="OO315_003770"
     CDS             complement(52..531)
                     /gene="xdhC"
                     /locus_tag="OO315_003770"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5892517.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="OO315_003771"
     CDS             complement(528..1406)
                     /gene="xdhB"
                     /locus_tag="OO315_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5892518.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="OO315_003772"
     CDS             complement(1417..3714)
                     /gene="xdhA"
                     /locus_tag="OO315_003772"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5892519.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALTEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRICSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            4129..4884
                     /locus_tag="OO315_003773"
     CDS             4129..4884
                     /locus_tag="OO315_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5892520.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4963..5036
                     /locus_tag="OO315_003774"
     tRNA            4963..5036
                     /locus_tag="OO315_003774"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4995..4997,aa:Gly,seq:ccc)
     gene            5171..6781
                     /locus_tag="OO315_003775"
     CDS             5171..6781
                     /locus_tag="OO315_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5892521.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6813..7420
                     /locus_tag="OO315_003776"
                     /pseudo
     CDS             6813..7420
                     /locus_tag="OO315_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1934 a         1860 c         1954 g         1855 t
ORIGIN      
        1 ttaaacacat aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt
       61 tttctcgcaa tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt
      121 tcccgccagt ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag
      181 cattgccgtg gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga
      241 tttcgcataa gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt
      301 gcggatctct tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc
      361 gtcgaccaac accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag
      421 cagtccttgt tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa
      481 aggcatcccg ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt
      541 ccccccgccg cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc
      601 agacgaaact ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat
      661 tcgctgatag cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca
      721 tgttggcagc gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa
      781 ttgccgttat ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg
      841 cgcatggcat atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga
      901 aaggcgacga ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca
      961 ttaatcggga cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt
     1021 agcgttggcg tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta
     1081 gcgacgttac ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga
     1141 tgacgttgag ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt
     1201 agcgagccat cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca
     1261 atatggcgat aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg
     1321 agcagtttgg cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg
     1381 cgatggtaag aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa
     1441 ctcttcatat aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat
     1501 cttcaccgcg ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt
     1561 atgtccgtat gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag
     1621 atccggcatg gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt
     1681 agcatcgatg atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg
     1741 tacctgacct tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat
     1801 gcggttgatg gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc
     1861 aaacgccggt gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg
     1921 ttcagggtgg tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg
     1981 tcgttcaacc agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag
     2041 catgactgcg gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc
     2101 aggcgcggca acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc
     2161 ggtatcttgt gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac
     2221 catttgcgag aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt
     2281 gatggttcca tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca
     2341 ggtgttagag gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg
     2401 gttctggcat tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca
     2461 ctccggcaac cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg
     2521 ggcggcgttg cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc
     2581 aagcatagac tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc
     2641 cgaggggagg ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag
     2701 gtaagcgact ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc
     2761 ggtgttagac agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat
     2821 ttgcccgtca atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct
     2881 aagggaaact ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc
     2941 ttccagtaca tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac
     3001 gcatgaccag ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg
     3061 ggtgctggaa acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct
     3121 ttccatgtga caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc
     3181 ggcatcgatt gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc
     3241 gccgttatgg attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg
     3301 taattcttgc cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg
     3361 ggccacgacg atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg
     3421 atcggcggta tcgcgcttgt tttcgtcaag tgtccaggca tgcccagctg tagcgaatgg
     3481 aatatcaggc acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg
     3541 ttcatcatta atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta
     3601 acacatgccc gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat
     3661 ggcatcgacg cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat
     3721 cccctcgatt tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta
     3781 cgccagaaac gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac
     3841 agatatgata cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg
     3901 ataaaatcaa tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag
     3961 tcaggaactt cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat
     4021 gaaattaacc tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga
     4081 ggctggctgc tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga
     4141 cgcctgaata aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg
     4201 ggctgttcgg gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg
     4261 aaacgggggg atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg
     4321 gcgcgactga acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg
     4381 ggtggggcga aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc
     4441 gcatcggtta caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt
     4501 tgttggttat ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc
     4561 aataaaggga ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga
     4621 aaggtggtgt atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac
     4681 agtgaagatt acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa
     4741 agcgtgaagg ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc
     4801 ctgcatttcc agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg
     4861 cagggcagca agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg
     4921 ctaaggagag cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg
     4981 tagaacgaga gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccatgac
     5041 acttcttaaa gtctactaac ttactgaatc ctaagggata cggcttaacg ccttccgata
     5101 gttttactca ctgtaaccaa cactgtgact aactttgttg aagtagtgag cagacctaag
     5161 gtctcagtac atgctcaagt caagaaccta cctttatcag cgtaatggtg tcttttacat
     5221 ccgtcttagg atgaagacca ctagtcgcct gaccgcttcg cttccttctc acaaccgtta
     5281 taaactagcg tcagtatctt tacggaccaa ggatagacgt accgctatgg ctcactcacg
     5341 gcacatcaag tcagcactta aagcaatcca tgcagacaac cctaacgcct cttatgagga
     5401 gctacgggag cacctgaaga ccattgtcga gtgggaactt agtgtcagcc gtgatgacct
     5461 gaacgacccg gagtcctacc agctttacgt tgaccagtac gatgacatta agtccaacct
     5521 tcgggaagct gtagcgaccg agcgtctgac tgtagaccag catcgctata ttaatgacgt
     5581 tatcggtgtg cttaaggcct gtcaggatag actcaatggc gatagctctg gcctattgtc
     5641 ttaccttgag cctgagactg gcagtctaag accgtccgtc tccctatctg tattggcgga
     5701 acctgaagtc cctgaaccta aagcactgac cctcgcgtct ctcattgagc agtacgagca
     5761 ggagaacgcc cagaactgga agccagcgac cctaagcgaa aaccgagcgt cacactctac
     5821 gctaatcgag atattcgact atctggatat tcaggatgtc ggcaaggcga cccgtgcaga
     5881 tatgctcaga gttcgtgaag tcctccagca gctaccgaag aaccgcaagc aacgctttaa
     5941 gtctatgccg ctgtctgact tgctgaaccg ggagtctaag actgactgtc tggacgtcgt
     6001 taccattaac aataaatatc ttatcaagat ggctgctgtg tttaagtggg cggtacgcaa
     6061 cgacttgata gctaagaacc tgaccgaagg tcttgagctg aaagtccctc agcgtaaagc
     6121 ctccgacgct cgtgatgcgt tcagtcctga gcaggtaggg caactactgg tcgcagctaa
     6181 agcgtactcg cagaagacct caggtaagcc ataccattat tacgtcaccg ctctggctgc
     6241 gattacaggt gcgagactta acgaggtggc ccaacttcag gttaaagatg tcaggaccac
     6301 tgaggctggg accgttttta tccatatcaa cgaagacgat agcagcctgc cgggtaagag
     6361 tatcaagaac gcccacagtg accgctgtgt cccgttggta gatggtgctt atggttttgt
     6421 attggctgac tttatgtcac tcgtggaaga ccgtagaaag actgagggtg ataatgctat
     6481 ggtgttcaat ggcctcaagc taatgaagaa cggctacggt gagcaggtta gcaagtggtt
     6541 taaccgaacg ttgctgccca aagtcctcgc tgaccgtagc ggcttagctt ttcactcgtt
     6601 tagacataca gttgcgaccc agttaaaaca acatggggtc gagttagcgt atgctcaggc
     6661 gattatgggt catagctcag ggtctataac ttacgaccgt tacgcgaaag aggtcgaggt
     6721 ggaaacgctg aaagaaaaat tagcggagtc attgtcagta aaaaaaatag acggaaagta
     6781 atcgcatagg gatagcggaa tgtgaggacg taatggtcaa agaattttta gttagcaaag
     6841 aaaataagcc ttcatatgaa tgtatgaata ctgctgaaac ttgtttgggg ctggctggaa
     6901 tggcttcgga agattggcaa cgtgagcatc attgccgcac aggtataata tttactgctt
     6961 ttgctattga ggcaatgttc atcttctata gaaaacaggt tgaccccggc tacgataaga
     7021 cgcagaaaga gtgcagaaag acaatgcaca agaatacgtt aaaattgtgc ggtataaaca
     7081 attatatggg gacaaaacct taccaaatta tcaaagagtg tcttgaggta agagacgcta
     7141 ttgctcacgg cgattcgtat acttcgagct ttaatttctc ggcggatcat ttggataacc
     7201 aagacgatat tgcaaaagtg tcctggcggt aaactctgaa caatttcggg gattaactgt
     7261 tgagcgcctt gcaaaataca ttgaaatggc aaagcatatt gaccatgaga tttctgataa
     7321 tggttacagg ccttctgaag gttaccttcc agcagccgag cgcagaagtc tgatgttagc
     7381 ttttggcgtc agtggtgtgt caatgtggcc cagtgcttga gcgataatac acctcactat
     7441 agaggaatgg acccttaact atcactatag gggagagacc ctaagtcccc accttaatag
     7501 cagtcacgac ccatacacac attcattaag tctcaggctt ccggtagcgg accgattgga
     7561 aaccatccgc agacgcctag tgttaaatca ccttggcctg aga
//
LOCUS       DAJTRD010000150         7541 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTRD010000150 DAJTRD010000000
VERSION     DAJTRD010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.150"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="OO315_003777"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="OO315_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="OO315_003778"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="OO315_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5892522.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="OO315_003779"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="OO315_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="OO315_003780"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="OO315_003780"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5892523.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="OO315_003781"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="OO315_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5892524.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO315_003782"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO315_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5892525.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="OO315_003783"
     CDS             complement(6510..7202)
                     /locus_tag="OO315_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5892526.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1916 a         1832 c         1986 g         1807 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccgggat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagaaaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc cccccccccg cgccgctgag aaaaagcgaa g
//
LOCUS       DAJTRD010000151         7518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTRD010000151 DAJTRD010000000
VERSION     DAJTRD010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.151"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..629)
                     /locus_tag="OO315_003784"
     CDS             complement(<1..629)
                     /locus_tag="OO315_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
                     /protein_id="HCR5892527.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHEL"
     gene            811..2235
                     /gene="sbcB"
                     /locus_tag="OO315_003785"
     CDS             811..2235
                     /gene="sbcB"
                     /locus_tag="OO315_003785"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5892528.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGE
                     PEVFYCKPADDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMKPLAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDID
                     TLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
     gene            complement(2278..2505)
                     /locus_tag="OO315_003786"
     CDS             complement(2278..2505)
                     /locus_tag="OO315_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5892529.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(2519..3577)
                     /gene="yeeE"
                     /locus_tag="OO315_003787"
     CDS             complement(2519..3577)
                     /gene="yeeE"
                     /locus_tag="OO315_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5892530.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(3756..5114)
                     /gene="plaP"
                     /locus_tag="OO315_003788"
     CDS             complement(3756..5114)
                     /gene="plaP"
                     /locus_tag="OO315_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5892531.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(5104..5166)
                     /gene="yoeI"
                     /locus_tag="OO315_003789"
     CDS             complement(5104..5166)
                     /gene="yoeI"
                     /locus_tag="OO315_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5892532.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(5327..6310)
                     /locus_tag="OO315_003790"
     CDS             complement(5327..6310)
                     /locus_tag="OO315_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5892533.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(6356..7180)
                     /locus_tag="OO315_003791"
     CDS             complement(6356..7180)
                     /locus_tag="OO315_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5892534.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(7263..7517)
                     /gene="yoeB"
                     /locus_tag="OO315_003792"
     CDS             complement(7263..7517)
                     /gene="yoeB"
                     /locus_tag="OO315_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5892535.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         2019 a         1957 c         1767 g         1775 t
ORIGIN      
        1 agttcgtgat acgacagtac ccgatcccct acctcagcgc gggagccgaa gtcccttggc
       61 tggatgctca taattgcgca actatctatc aactgacgcc gcatgcacca actaattgct
      121 gatcttagtt gacttagcac ctgccttgct cggcgtggat tatctttttc ttcttcggta
      181 agcaggtcaa cccattgctt aaccgtgata gaagatgccg gacgattagg gaaggcgtca
      241 tgcatgcgct tcataaccgt tgatcgataa agtgcctggg tcttttctct gagagttgta
      301 gagacgtagt tgtcgaacca gtagtcgaga cactgggcga ccgtcatgga gttctccacc
      361 ttctcttcaa aataggtgcg tggatccgtt cctgagaaat agagctttcg caagtcagca
      421 gtgatctgtc tggcatcctt caaagacagg gatgggtatc gaccaagccc aagtcgatta
      481 ggcttgccat gccagcgata gcggtactgg aactggatga cccccttcgg tgaaattcgt
      541 acgctgaggc catcggcatc agccacttct tgtgggcccg aatatggttt accataaata
      601 gtacgcagtt ttgtgtcgct tatagccata aataatattt ggtacgctca gaaataatag
      661 tttggcacac atctggtaca caatatcaca tggacaacag cgcacagtaa acaactatat
      721 ttgacgttcg tagacatact caggtgaaaa aagggttgct tttcgagtta taacagacag
      781 ttaattaact acagtggaca atgctagaca atgacagaca cagataaaca acctaccttc
      841 ctctttcacg attacgaaac ctttggcacg caccccgcgt tagatcgccc tgcacagttc
      901 gcagccattc gcaccgatga cgaattcaat gtcatcggcg aacccgaagt cttttactgc
      961 aagcccgcgg atgactattt accccggcct ggagcagtat taattaccgg tattaccccg
     1021 caggaggcac gggcgaaagg agaaaacgaa gccgcgtttg ccgcccgtat tcactcgctt
     1081 tttaccgtac cgaagacctg tattctgggc tacaacaatg tgcgtttcga cgacgaagtc
     1141 acacgcaacg ttttttatcg taatttctac gatccttacg cctggagctg gcagcatgat
     1201 aactcgcgct gggatttact ggatgttatg cgtgcctgtt atgccctgcg cccggaagga
     1261 ataaactggc ctgaaaatga tgacggtcta ccgagctttc gccttgagca tttaaccaaa
     1321 gcgaatggta ttgaacatag caacgcccac gatgcgatgg ctgatgtgta cgccactatt
     1381 gcgatggcga aactggtaaa aacgcgtcag ccacgcctgt ttgattatct ctttacccat
     1441 cgtaataaac acaaactgat ggcgttgatt gatgttccgc agatgaaacc cctggctcac
     1501 gtttccggaa tgtttggggc atggcgtggc aataccagct gggtggcacc gctggcgtgg
     1561 catcctgaaa atcgcaatgc cgtaattatg gcggatttgg caggagacat ttcgccatta
     1621 ctggagctgg atatcgacac attgcgcgag cgtttatata ccgcaaaagc cgatcttggc
     1681 gataacgccg ccgttccggt taagctggtg catatcaata aatgtccggt actggcccag
     1741 gcgaatacgc tacgcccgga agatgccgac cgactgggaa ttaatcgtca gcattgcctc
     1801 gataacctaa aaattctgcg tgaaaatccg caagtgcgtg aaaaagtggt agcgatattc
     1861 gcggaagccg aaccgtttac accttcagat aatgtggatg cacagcttta taatggcttt
     1921 ttcagtgacg ccgatcgtgc agcaatgaaa attgtgctgg aaaccgagcc gcgtaattta
     1981 ccggcactgg atatcacttt tgttgataaa cgaattgaaa agttgttgtt caattatcgg
     2041 gcacgcaact tcccggggac gctggattat gccgagcagc aacgctggct ggagcaccgc
     2101 caccaggtct tcacgccaga gtttttgcag ggttatgctg atgaattgca gatgctggta
     2161 caacaatatg ccgatgacaa agagaaagtg gcgctgttaa aagcactttg gcagtacgcg
     2221 gaagagatcg tctaatttga agccgccgcc gctggtacgg cggaggacaa tgttgagtca
     2281 ggctttttga acggtgatgc tccacgctgc atcgccaatc tgctgataat cggtgatggc
     2341 atgtccttct tctgccgccc actgtgggat tgcttcagtt gcctgggtgc aatcaaactc
     2401 aattaccaac tcatcgccgc tggccatttc tgccagtgct gcttttgctt ctatgagtgg
     2461 aaacggacag acctgcgtca ccacatccag ctttttaatc gccatcttta caccttactt
     2521 aatttgccgc agcagttgcc agtcgcgcct tacgctgcgg tcgaacatac acaagccagg
     2581 acgcagtcca gactccgaga atcataaata caaggccaat ccagccctgc caggtcatca
     2641 ttgcagtcat aaccagtcca ttaccgatag agcaaccacc tgcaatactg gcaccgaagc
     2701 ccatcagtac accacctaac ccgctacgta atgttgtttg tgcatcagct gcgcgaacgc
     2761 ggaactcacg gctcgctttg gctgcaataa atgcctccac gaagatccct aacaccagga
     2821 aaacgcccca gttaatgtat ttcacgtcac ccgcgaccag aaattgaaga atattggccg
     2881 ttggggaagt gactcctaac ccaaacatgc gtccggttgc ttcactcagg ggccaggcta
     2941 aaagcgcaat caaaccgatg agtacagcag tgacaaaggg atgccagcgt ttttcaaaca
     3001 gaatatgagc gatcccggtt cggcgcggcg gtaaggtcgc gactttgagt tttggcttct
     3061 tcaactcttt catcaccacc cagagcgtta tcaccagcaa aacggtaacc aacggccaca
     3121 cagacaaatt aaaagtctca gcaatagagt tatgttcagt agtgtagtgc tgcaaggttt
     3181 gatttaaacc actggcgtgt ggagaacgca tcaccgcact catcaccata taagtgaaaa
     3241 gcgcgatcca actgccgatc aatccctcac ccgcgcgata ccaggtcccg gtggcacatc
     3301 cacccgccag aacaattccc agcccgaaaa gatacccacc tataacagta ccaagccacg
     3361 ggaacgcccc ggcttcgtaa gtcagtaggc ccgcctgaat taacgcaaaa acccccacgc
     3421 tttgtgccga aattgcaatc agcaaggcgt aaaacatgcg attatttttc gcgatataca
     3481 tatcgcgaaa accaccagtc aggcaaaaac gcccacgctg catgacaaat cccagcagag
     3541 caccacaaat tagcccgctt aatatcattg aaaacataaa ttaattaacc agatgaatgt
     3601 taatggggaa atttttcatg actggtggaa tgcagaccaa taaccaaatt ctctaaatta
     3661 gaacaaatgg ttattaatga ggatttcgat aaaaaagaat aaaaaaaccg gagccaaatg
     3721 ttcattcgac tccggtttat tattagaaaa gatgattatg ctacgtcttc gtactgcgga
     3781 accggattac ggaagctctt ggtgacgcaa gccaggtaaa tcagaccgat agcagcccag
     3841 atcagaccca gaaccattga gctttcttcc aggttaaccc acagcgcacc aacggtcagc
     3901 gcaccacaca tcggcaggaa cagatactgg aagtgatctt tcagcgtctt gttacgcttc
     3961 tcacggatcc agaactgcga gatgaccgac aggttaacaa aggtgaacgc caccagcgca
     4021 ccaaagttaa tcagcgccgt tgccattacc aggtcgaagt tgattgccag cagggcaatc
     4081 gcgccaacca ggatgatgtt catcgccgga gtacgccatt tcgggtgtac ataaccgaag
     4141 aagcttttcg ggaacacgcc gtcacgaccc attacgtaca tcagacgcgc tacgcctgcg
     4201 tgcgccgcca taccggacgc cagtacggta atggtggaga agatcagcgc gccaacctgg
     4261 aacgctttac ccgcaacgta cagcatgatt tcaggctgtg acgcatccgg atctttaaag
     4321 cgagagatat ccgggaagta cagctgcagg aagtaagttg caaagatgaa gatcatgccg
     4381 ccaatcagcg cggtcaggaa aatcgcacgc gggatcacgc gctctgcatc tttggtttct
     4441 tccgacagat tgctgatgcc gtcaaagccg gtaaaggaga agcacaggat tgtcgccccg
     4501 gtaatcatcg ggataacatg cgcatcgcca gaccagaacg gacgcgtgct cgccagcgta
     4561 ccagcgcctt caccttcaaa tacgccataa acaaccatgc ccagaatcac cgcgatcagc
     4621 actacctgca acacgacgat tacagtgttg acgttcgcta cggatttcag actacgcagg
     4681 ttaaaggcgg tcataaaggc caccagcgcc accacaaaca tccacgatgg aatggaaggc
     4741 accagagctt caaaatagat tttcgccagc agaatgttga tcatcggcgc gaacagatag
     4801 tcgagcagag aagaccaacc caccataaag ccgacagtcg ggctaatgga tttctgggcg
     4861 taagtgtatg cagagccagc agaaggatag cggcgaacca gcttcccgta gctcagagcc
     4921 gtaaacagga tcgcaatcaa tgcgaacgca taggctgtcg gcacatgacc atccgtgagg
     4981 cctgaaacga taccaaatgt atcaaacagc gtcatcggct gcatataggc tagacccatc
     5041 attacaaccg gaactaacgt aagcgtttta cgcaattcca cgcgagaggt gtttggagta
     5101 acgttatgcg acatggttat tctcctttac ggtgataacc gtcgcgtaag caaaaaattg
     5161 ccccattttt ttggattcct cagcgacaac aactgtcgat ttttagtaaa tatctatccg
     5221 gtacgaagcc cggcctcttg gtatgaatga ttggtttgaa gcaaaaaata accgacgcta
     5281 attaaacgtc ggtttttaat cattttttga cagcggcgca ttgtgcctaa atggggggga
     5341 aatgacaaga gaatgagagg cttgtcataa taatttttct ttgaatggct gattttccgt
     5401 catcagattt tgcataaaca ccggcgatcg cactatttgc taaaatttca tctcgccacc
     5461 agccgctggc aagccctgcg gtctgttcat tccagccaat ccagacaggt tcaaagagag
     5521 tttgggcgac cactttcttc tcgattaacg cgccactatc gagaaaacgt tgcgccagat
     5581 accgtggtaa atagccgcag ccaaggccgc taatttgcag ctccagtttg gttttaaaat
     5641 cgaaaacggt aatggcttct tgttcgtcga gaagttgtga ggccgtagaa gcgcctgcct
     5701 gcgcagtatc ccccaccaca atcgcccgat atcgtttaat gatacgacgg tttaacggtt
     5761 cttcttcctg ggctaacgga tgatgaggcg cgaccgcaaa gacttgttca agatcgccca
     5821 accgcgagaa accaaactca ctggaagatg gtggctcatg catcgcgcca acgataatat
     5881 ccgctcgccc ctgagtcagg gcatcccagg aaccaccgag tacgccattg ataaacttca
     5941 ggcgcgtcac gctatgatgt tgataaaaag cttcaatgag aggcgcaagc agagaaaaag
     6001 gaaacgtgtc gtccacgcca atcaccagct cgttttccca gccttcatgc agtttgatcg
     6061 cctgcttttc cagttctcgc acggtatgca gaacttcccg ccctttctct aataacattt
     6121 ttccggtgcg ggtgaattta gcgcggtgtc cgctacgatc aagcaattga atatttaggt
     6181 cgctttccag cttgtgaacg gtgtaactca gagcggacgg cgtcttgtag agtttggctg
     6241 acgccgccgc aaaactgccc tctttttcta tggcatcgag gatcatcaac acatccagca
     6301 gtggtttcat acacgccccc ttgcagtgca tgcggcgtcc tgctggcgtg atcgtttact
     6361 ccaacggcat taccagcgga tcgggatact ggtattcaaa tcccagttca ttacaaatcc
     6421 gactgccatc aataatctta cctttgccgc tgtccagact atttctgaac tgcggaggtt
     6481 gcagccccag taaacgggcc atctgcggat agaaaacatt acgcgcaggg tgagctggcg
     6541 cacatatatt atagatgtgt ccgcctttag gtgcctgtaa caacagagtg atagcgccaa
     6601 taacatcttc taaatggact aaattaacac catgttcacc atcaggggcg gtttttccgg
     6661 caaagaagcg tcccggatga cgtcccggtc cgaccaggcc cgcaagacgc agaatatcga
     6721 ccgaagtacc gggtaaattg tgcagccagt cttcgagttc ttctaatact cgtccactgt
     6781 tggttaccgg attacgcggg gtagtttctt taaccgtgcc ttgcgcgtcg ccatagacag
     6841 atgttgagct ggtaaaaata attcggggaa tacgatgggc cagcgcgcta tccactaact
     6901 cttgtaccgc ttgtaaatag aactcatcgc cggggccgct acgacgtgcc ggaagcgtaa
     6961 tgaccagcgc atcggcatcc ataagggcat ccagatcgtc agaatcgcaa actaactcag
     7021 gctccatgcg aagcagatag ctatcaatgc cactcattcg ggccgcttcg acaccatctt
     7081 gtgtggtttt actcccggtg acttgccagc ctcgcgctga aagtgacatc gccagcggca
     7141 tacctaacca ccctaacccg acaattgcga cctttttcat ccgttatctc ctgatacacc
     7201 ttagatctat aaggctacgc tagcgtatca aaactgacaa ttcattctat gaatgaatct
     7261 gttcaataat gataacgaca tgctgcaatg agcagtgaat catcagtaac cgcgtatacc
     7321 agacggtgct cctctgtaat gcgtcgggac cagaaacctg acaaattatg tttcaggggt
     7381 tctggtttcc ccttaccttc aaatggtgtt ctgcgggtat ctttgataag ttcattgatc
     7441 tttttaacaa ttcgcttatc tgtttcctgc cagtacagat aatcatccca tgattcctca
     7501 gaccagatta gtttcact
//
LOCUS       DAJTRD010000152         7376 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTRD010000152 DAJTRD010000000
VERSION     DAJTRD010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7376
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.152"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            53..1543
                     /locus_tag="OO315_003793"
     CDS             53..1543
                     /locus_tag="OO315_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5892536.1"
                     /translation="MDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAIEAAQKS
                     TPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGIPLTETF
                     LPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDY
                     FMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAY
                     NAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTII
                     LFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDF
                     YPTALDAADISIPKDLKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWD
                     NYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDN
                     LAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1595..2752
                     /locus_tag="OO315_003794"
     CDS             1595..2752
                     /locus_tag="OO315_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5892537.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2718..2840)
                     /locus_tag="OO315_003795"
     CDS             complement(2718..2840)
                     /locus_tag="OO315_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892538.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3043..4728
                     /locus_tag="OO315_003796"
                     /pseudo
     CDS             3043..4728
                     /locus_tag="OO315_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4766..7126
                     /locus_tag="OO315_003797"
     CDS             4766..7126
                     /locus_tag="OO315_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5892539.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            7183..>7376
                     /locus_tag="OO315_003798"
     CDS             7183..>7376
                     /locus_tag="OO315_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892540.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGS"
BASE COUNT         2164 a         1615 c         1567 g         2030 t
ORIGIN      
        1 tacgccgact gaatacagta ccaaaggaaa gccaaatatt atcgtactaa ccatggatga
       61 tcttggttat ggacaacttc cttttgataa gggatctttt gacccaaaaa caatggaaaa
      121 tcgtgaagtt gtcgatacct acaaaatagg gatagataaa gccattgaag ctgcacaaaa
      181 atcaacgccg acgctccttt cattaatgga tgaaggcgta cgttttacta acggctatgt
      241 ggcacacggt gtttccggcc cctcccgtgc cgcaataatg accggtcgag ctcccgcccg
      301 ctttggtgtc tattccaata ccgatgctca ggatggtatt ccgctaacag aaactttctt
      361 gcctgaatta ttccagaatc atggttatta cactgcagca gtaggtaaat ggcacttgtc
      421 aaaaatcagt aatgtgccgg taccggaaga taaacaaacg cgtgactatc atgacaactt
      481 caccacattt tctgcggaag aatggcaacc tcaaaaccgt ggctttgatt actttatggg
      541 attccacgct gcaggaacgg catattacaa ctccccttca ctgttcaaaa atcgtgaacg
      601 tgtccccgca aaaggttata tcagcgatca gttaaccgat gaggcaattg gcgttgttga
      661 tcgtgccaaa acacttgacc agccttttat gctttacctg gcttataatg ctccgcacct
      721 accaaatgat aatcctgcac cggatcaata tcagaagcaa tttaataccg gtagtcaaac
      781 agcagataac tactacgctt ccgtttattc tgttgatcag ggtgtaaaac gcattctcga
      841 acaactgaag aaaaacggac agtatgacaa tacaattatt ctctttacct ccgataatgg
      901 tgcggttatc gatggtcctc tgccgctgaa cggggcgcaa aaaggctata agagtcagac
      961 ctatcctggc ggtactcaca ccccaatgtt tatgtggtgg aaaggaaaac ttcaacccgg
     1021 taattatgac aagctgattt ccgcaatgga tttctaccca acagctcttg atgcagccga
     1081 tatcagcatt ccaaaagacc ttaagctgga tggtgttccc ttgctgccct ggttgcaaga
     1141 taagaaacaa ggcgagccac ataaaaatct gacctggata acctcttatt ctcactggtt
     1201 tgatgaggaa aatattccat tctgggataa ttaccacaaa tttgttcgcc atcagtcaga
     1261 cgattacccg cataacccca acactgagga cttaagccaa ttctcttata cggtgagaaa
     1321 taacgattat tcgcttgtct atacagtaga aaacaatcag ttaggtctct acaaactgac
     1381 ggatctacag caaaaagata accttgccgc cgccaatccg caggtcgtta aagagatgca
     1441 aggcgtggta agagagttta tcgacagcag ccagccaccg cttagcgagg taaatcagga
     1501 gaagtttaac aatatcaaga aagcactaag cgaagcgaaa taactaaacc ttcatgcggc
     1561 ggatttttcc gccgccttat tgagcgagat agcgatgcac gttacagcca agccctccag
     1621 ttttcaatgt aatctcaaat gtgattactg tttttacctt gaaaaagagt cgcagtttac
     1681 tcatgaaaaa tggatggatg acagcactct gaaagagttc atcaaacaat atatcgaagc
     1741 gtctggcaat caggtctatt ttacctggca aggcggtgaa cccactctgg ctggcctgga
     1801 ttttttccgt aaagttattc actatcaaca acgctatgca ggccaaaaac gtatttttaa
     1861 tacattacaa acgaatggca ttttattgaa taatgaatgg tgtgcctttc tcaaagaaca
     1921 tgaatttctg gttggtatct cgatcgatgg cccccaggag ttacatgacc gttacagacg
     1981 cagtaattca ggtaacggta cttttgcaaa agtgatagca gccatcgagc gtctgaaatc
     2041 atatcaagta gagtttaata cgttaaccgt cattaataac gttaatgtcc attatcctct
     2101 tgaggtttat cattttttaa aatctatcgg cagtaaacat atgcaattta tcgaattgct
     2161 agaaaccggg acgccgaata ttgatttcag tggtcatagt gagaacacat tccgtatcat
     2221 tgatttttct gtgcctccca cggcttatgg caagtttatg tcaaccattt ttatgcaatg
     2281 ggttaaaaac gatgtgggtg aaattttcat tcgtcagttt gaaagctttg tcagccgttt
     2341 tttggggaat gggcatacaa gttgtatttt ccaggagtcc tgcaaggata atctggttgt
     2401 tgaaagtaat ggagacattt acgaatgcga ccattttgtc tatccacagt acaaaattgg
     2461 aaacattaat aaatctgaac tcaaaacgat gaacagtgta caactgacag cgcaaaaaaa
     2521 acggatttca gcgaaatgtc agcaatgtgc atataaacct atctgcaatg gcggttgtcc
     2581 taagcatcgt attactaaag taaacaatga gactgtttct tatttttgcg aaggttataa
     2641 aatccttttt tcaaccatgg taccttatat gaacgccatg gttgagttag ctaagaacag
     2701 agtaccgctt taccacatta tggatgttgc aaaacaaatg gagaataatt aataacgctc
     2761 tcttttaact ccattcttag cctccttctt atgaccttaa caaggcaccc gacataaatt
     2821 cctgtcgggt gtttcgtcat catcatcttc ccttgcgaaa atcctggctt acgtggtatg
     2881 cgtcagggca ttagcaatac ccttgattat caatagcata accataacca ctgaaaatta
     2941 aaaatttcta tatatttcat attgttaaat gatgcttaaa taaatttcat cagtaaatca
     3001 ttgccataga taatcattgg atggcacaat caattttcgc taatgataac cattcccatt
     3061 acgttttgta tgttgatagc aaagtatctc tgtctgctga agcccttttg gctgcgtaag
     3121 aataataaaa cttcagtgtt gttaatcatt attatcctcg caatgatcct tggcgtggtt
     3181 aaaattcagg tctggttaaa cgactggaat aatgattttt tcaatgcgct aagccagaaa
     3241 gaaaccgata agctttggca gctcgttctt tggttccctg ccctgctggg gatctttgta
     3301 ttaatatccg caaacaaaac atggttaatt aaactactga ctattcgctg gcgcgaatgg
     3361 ttaacagatt actaccttaa tcgctggttt gcagataaaa actactactt cacgcagatc
     3421 tacggcgagc ataaaaatac agataaccct gaccaacgta tcgctgaaga cattctctta
     3481 ctgatcaaca aaacgctcag tctttcattt ggcttcatcc agtcacttag catgctgatc
     3541 acctttaccg ttattctttg gcaaagcgcg ggtacgctct cattcactgt tggtggaaca
     3601 gaatggaata tccaggggta tatggtctat accgtcgtgc tcattgtgat cggtggaact
     3661 ttatttaccc ataaggtggg taaacgtatt cgtccgctta atgtggaaaa acaacgtagc
     3721 gaagcgacat ttcgaactaa tcttgtgcag cataataagc aggcagaact gattgcgcta
     3781 agcaatgctg agtctctaca acgccaggag ttgcgcgaca attttcatac cattaaagag
     3841 aactggcatc gtttaatgaa tcgtcagcgg tggcttgatt actggcaaaa tatttattcg
     3901 cgctcgctta gcgttcttcc ctactttctg ttattgccgc agtttattag cgggcagatc
     3961 aatctgggcg gactgatgaa atcgcgccag gcatttatgc tagtatcgaa caatttaagc
     4021 tggtttattt ataaatatga cgaacttgct gaactggctg cggttatcga tcgcttgtat
     4081 gagttccatc aactcactga atagcgccct acgaataagc ctaaaaattg ccaacatgcg
     4141 gtacaagtgg ctaatgcgag tattcgtacg cctgataata agatcatatt agagaacttg
     4201 aactttcatg tttcgccagg caaatggcta ttactgaaag gctactctgg tgcgggaaaa
     4261 accacactgc ttaaaacatt atcccactgc tggccgtggt ttaaaggtga tatttcttct
     4321 cctgctgaca gttggtatgt gtcacaaaca ccgttaatca aaagcggctt actgaaagag
     4381 attatttgta aagcacttcc cctgcccgta gacgataaat cgttgagcga agtactgcat
     4441 caggttggtc tgggaaaatt ggctgcgcgt attcatgacc acgatcgctg gggagatatt
     4501 ctttccagcg gcgaaaaaca acgtatcgcc ctggcacgat taattttacg acgtccgaaa
     4561 tggatatttc ttgacgaaac tacctctcat cttgaggaac aagaggctat acgcttactg
     4621 cgtttagtgc gtgaaaaact acccacaagc ggcgtcatta tggttacaca tcaacccggt
     4681 gtctggaacc tggctgatga tatttgtgac attagcacgg ttttataaca acaataaaag
     4741 ccctgctgta cggagatata aataaatgaa gcgagttctt attcctggcg tcattttatg
     4801 tggcgctgat gtggcgcagg ccgtcgatga caaaaacatg tacatgtatt tttttgaaga
     4861 gatgacggtc tatgctcctg tccctgtacc cgtaaacggc aacacgcatt acaccagtga
     4921 aagcatcgag cgtttaccga ccgggaatgg caatatcagc gatctgctga gaaccaaccc
     4981 tgcggtacgc atggattcaa cgcaaagtac ctcgttgaac cagggagata ttcgccctga
     5041 gaaaatctct attcacggtg cgtcgcccta ccagaatgcc tatttgattg acggtattag
     5101 tgcaactaat aacctgaacc cagcgaatga gtccgatgcc agtagtgcaa ccaatattag
     5161 cgggatgtca caggggtatt atcttgatgt cagcttactg gacaatgtga cgctttatga
     5221 cagttttgtg ccggttgaat ttggtcgctt caatggcggg gtaattgatg caaagatcaa
     5281 acgcttcaac gctgatgata gcaaggtgaa attgggttat cgcactacgc gtttggactg
     5341 gttaacatcg catatcgatg agaataacaa gagcgcattt aatcaaggtt cttcaggaag
     5401 tacctatttc tctccagatt ttaaaaagaa cttttatacc ttgtcgttta atcaggagct
     5461 ggctgataac tttggcgtta ccgccggttt atcgcgccgc cagtctgata tcacccgcgc
     5521 ggattatgtt tcgaatgacg gcattgtcgc cggtcgggca cagtataaaa acgttatcga
     5581 tactgcattg agcaaattta cctggtttgc cagcgaccgc tttacccacg atttaacctt
     5641 aaaatatacc ggctccagcc gtgattataa taccagcacc ttcccgcagt ctgatcgcga
     5701 aatgggtaat aaatcctatg gtctggcatg ggatatggat acgcaactcg catgggccaa
     5761 actacgtacc accgttggtt gggatcatat tagtgattat acccgtcacg atcatgacat
     5821 ctggtacacc gaactttcat gtacatatgg tgatattaca gggcgttgta cccgtggcgg
     5881 attaggacac atttcccagg ctgtagataa ttacaccttc aaaacacgcc tggactggca
     5941 aaaattcgcc gtgggtgatg tttcgcatca accctacttc ggcgcggaat acatctattc
     6001 cgatgcatgg actgaacgcc ataaccagtc tgaatcctat gtgattaatg ctgcaggaaa
     6061 gaaaactaac cataccattt accataaagg taaaggcagc ctgggaattg acaactacac
     6121 gctgtatatg gcggatcaca ttagctggcg gaatgtgtcg ttaatgcccg gtgtgcgtta
     6181 tgactatgac aactatctgt caaaccacaa tatctccccg cgctttatga cggaatggga
     6241 tatttttgct gatcaaacct caatgattac cgccggttat aaccgttact atggcgggaa
     6301 tattcttgat atgggattac gtgatatccg caatagctgg acggaatcgg tatcaggtaa
     6361 taaaaccctg acgcgttatc agaatttgaa aacgccttat aacgatgaac tggcaatggg
     6421 attgcagcaa aaaatcgata agaacgttat tgcacgcgca agtgaagcgc atgatcaaat
     6481 cagcaaaagc agtcgtaccg acagcgcgac taaaaccacc attactgaat ataacaacga
     6541 tggcaaaacc aaaacgcatt cgtttaacct cagttttgaa ctggccgaac ccctgcatat
     6601 ccgccaggta gatattaacc cgcaaattgt ctttagctat atcaagagca agggcaactt
     6661 gtcgttaaac aatggttatg aggagagcaa taccggtgat aaccaggtgg tttataacgg
     6721 taatctggtc tcttacgata gcgttccagt ggcagatttt aataacccat taaagatctc
     6781 cttaaacatg gatttcacgc atcaaccgag cgggttagtg tgggcgaata cgctggcctg
     6841 gcaagaagcg cgtaaagctc gcattatcct tggtaagaca aatgcgcaat acatcagcga
     6901 atattcagat tacaagcagt atgttgacga aaaactggat agcagcctga cctgggacac
     6961 ccgcttgtcc tggacgccac aatttctgaa acaacaaaac ctgacgatca gtgccgatat
     7021 tctcaatgta ctggatagca aaaccgctgt tgatacaacg aacaccggtg tggcgaccta
     7081 cgccagtggc cgtactttct ggcttgatgt cagcatgaaa ttttaatagt tcggatttta
     7141 cttaaccggg taaattcgcc cggttttcgc atggagataa ttatgagaaa cctctgtttc
     7201 ttactgacgt tagtggcaac tctgttgctc cccgggcggc tgattgccgc cgccttaccg
     7261 caggatgaaa agttaattac cgggcaactg gacaatgact tgcgatatat gatttatccg
     7321 catgctcatc caaaggatca ggtaaattta tggctgcaaa ttcataccgg ttcatt
//
LOCUS       DAJTRD010000153         7188 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTRD010000153 DAJTRD010000000
VERSION     DAJTRD010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7188
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.153"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(15..708)
                     /gene="ospZ"
                     /locus_tag="OO315_003799"
                     /pseudo
     CDS             complement(15..708)
                     /gene="ospZ"
                     /locus_tag="OO315_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            764..916
                     /locus_tag="OO315_003800"
                     /pseudo
     CDS             764..916
                     /locus_tag="OO315_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2297..3244
                     /gene="icsP"
                     /locus_tag="OO315_003801"
     CDS             2297..3244
                     /gene="icsP"
                     /locus_tag="OO315_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5892541.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3806..4178)
                     /locus_tag="OO315_003802"
                     /pseudo
     CDS             complement(3806..4178)
                     /locus_tag="OO315_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4422..4541
                     /locus_tag="OO315_003803"
                     /pseudo
     CDS             4422..4541
                     /locus_tag="OO315_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4791..5657
                     /gene="ospB"
                     /locus_tag="OO315_003804"
     CDS             4791..5657
                     /gene="ospB"
                     /locus_tag="OO315_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5892542.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5986..6726
                     /gene="phoN2"
                     /locus_tag="OO315_003805"
     CDS             5986..6726
                     /gene="phoN2"
                     /locus_tag="OO315_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5892543.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7086..>7188)
                     /locus_tag="OO315_003806"
     CDS             complement(7086..>7188)
                     /locus_tag="OO315_003806"
                     /inference="COORDINATES: protein motif:HMM:NF017898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5892544.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFFIKKWSGIRQTI"
BASE COUNT         2252 a         1356 c         1384 g         2196 t
ORIGIN      
        1 acaattaata tacattaagt aacaggcatt cgagccttga tttgagaaag cctgtcattc
       61 atttgtttta tgtagctatc tatttcctct gatgcataac taaaaagttc tcccatttca
      121 cgagcaataa taatctcaga tttaatagac tttaatctct ggcgactcat ctcttaacac
      181 ttgcataatc tctcttttta tatcatatag ttctataaac cgatttgccc gtagttctcc
      241 ttgggaagaa tcatttctga atacaacaat tatatacaaa aaatcactat tttctttaga
      301 gaagatctta caactaatct ctgggagctc acataatgca tgtaagtcag gatggtacaa
      361 actatccaca gaaaattgcc ccataggacg ttttccagat ataactgtca accagcttcc
      421 ttccggtgta ctggcttcgg ctctggaaat aggctgaaaa tcttccggac atggagaata
      481 cattgtcttc atataattaa gtcgtctgga tttctcatta ataaactcct ctatttttaa
      541 ttgtgccata cttctggtta taccagagat atacttcttg tcatatatgt ttggtgattc
      601 attaaagtaa ccatgttcaa ctctaggata aatatttctc actatatatt cagtatttgc
      661 agtatttatt ggaaatacgt tcttaatatt cttgatggga ctaatcattt taatctctat
      721 actcaaatat aaacattacc atgaacaata taattgttcc taagtggaat gtctccacgg
      781 agaacacttt atctatcggg aaatagtgcg ggcaacagtg tttaattata tcgagtgcaa
      841 ttacaatcgc tggcgacggc accgttggtg tggcggcctc agtccggaac aatttgaaaa
      901 ccagaacctc gcttaggcct gtgtccatat tacgtgggta ggctcacaga gcctcactta
      961 cgactgcgtc catattatgt gggtaggatc aataaagacg ttttatatgt gtctgcttgc
     1021 agccagaaaa tttataccct gcaggtatca taaacatgct tgataactta attggggctc
     1081 cctcttcctt ttatggtgta attgtttgat tgaatacttc cggggatttc agtatgaaat
     1141 gaagtatatt taatatactt tacaaaaagt ttgtattagt ggaataagga caatagctta
     1201 tccaaactgc caagcctctt tattataagt aagatctggc actggaatat tctcaaaagg
     1261 actaacatca tcaccacgac cttcaaccaa ccattttatt gcatttaaat cccagaatag
     1321 ttcagtagtg gtcaatttgg gaaacgcaca atattgtacg gttacgttat cgcaatagag
     1381 tgaactctcg ccctatacgg cgttttcaag gattagtcct taatcggaca accacattaa
     1441 agcataataa ataattaagc tttaataata tactatagat acagttctat tataaaaaat
     1501 actattttag gtgatgcatt aaattagtag aatatttatt cttttataaa agcaccaatg
     1561 aattgtccaa gtaaacatga gataaaatgt gtagaggata actgagtttg gtcactttaa
     1621 ctgtattagt cgcagtctga ctctcgactt taaaaggatg ggaattacct gtactgatag
     1681 tgagaaagct ttccattgaa ataagtcaaa taaaatttga tggcttcagt gctaaagtga
     1741 ggtgcccgct agccgtattc actcgaatcg gaattcaatg tattgcttct gccatttcca
     1801 taatatttca cgggaaaaac aaaatctgaa aaactatact taagtgttgt attgagtcag
     1861 ttgagataca aatacatatt tatcagcaca cgttacgtca aatacaggag gttgtcatgt
     1921 tggtactgaa aggcacgttg gctattgcag ccgtgatgac aatttattgt cttatcgttg
     1981 ttcttatgga acgcttttct gattgatttc atattggcga gagaacaaga aggtgagtag
     2041 aattgtcgcg gaatcctgaa actatcagcc tcaggtatga acaccaaaag gtaggtagag
     2101 aaatctatgg cccccgttac tttattcgca caatgaggta tttttttatt tttacaaaag
     2161 ttcaataaat attataataa aagatatgtt cttggtaaca taaacaaata tttaattaaa
     2221 ataatgcttg gcattgacgt ttatcttata aagtaagaag atcatggaca tttcaaccaa
     2281 aaaagtagag ttctcgatga aattaaaatt ctttgtactt gcactttgtg tacctgcgat
     2341 ctttactaca catgctacca ctaactatcc acttttcata ccggacaaca tcagtacgga
     2401 tattagtcta ggatctctga gtggcaaaac aaaagaacgc gtttatcatc ccaaggaagg
     2461 ggggagaaaa attagtcaac tggactggaa atacagtaat gcgactattg ttagaggtgg
     2521 catcgattgg aagctaattc caaaagtgtc tttcggagtt tccggttgga ctactttagg
     2581 taaccagaaa gcaagcatgg ttgataaaga ctggaacaat tccaataccc ctcaggtatg
     2641 gaccgaccag agctggcatc ccaatacgca tctccgtgat gctaacgaat tcgagctgaa
     2701 tcttaaaggt tggttattaa ataatttgga ttatcgactt ggactaatag caggttacca
     2761 agagagtcgt tacagtttta atgcaatggg agggagttat atttatagtg aaaacggggg
     2821 gagcaggaat aaaaaagggg cacatcctag tggtgaaaga acaataggtt acaaacagct
     2881 cttcaaaata ccttatattg gattaactgc taattaccgc catgagaatt ttgagtttgg
     2941 agcagaactg aaatatagtg gttgggttct ttcatctgat acagataaac actatcagac
     3001 tgagacaatt tttaaagatg aaataaaaaa ccaaaattac tgctctgttg ctgcgaatat
     3061 tggatactat gtcaccccca gtgcaaaatt ttatatagaa ggctccagaa attacatttc
     3121 taataaaaaa ggtgatacat ctctttatga gcaaagtacc aatatatctg gcaccattaa
     3181 aaatagtgca agtattgaat atattggttt tctcacttcc gcaggtataa agtatatttt
     3241 ttgaattggt tcaccgagtt gattacgttt tcatggctaa aaattgccgc ccattgtgag
     3301 tcatattcgc cctgactttc cagaaccata cgaactgccc gttgacggac ttcgggggaa
     3361 aaacgtgtat ttttagtcat tctgtttacc tctttctcag ggcgtttagt ctccaggatt
     3421 tccggggcgg ttcaaaaaac tcttcgttga ttactgtgga cttactgttg gcattaccga
     3481 ccctgagacc ggagaaataa gaaccgctta gatcatcgtg gctgttctcg gggtatcaag
     3541 ttacacatgg gcagaggcta cccaaggtct actattatcg cctgaaagcg ctgatggaac
     3601 agtgctatca gggacatgct gatgaaaata cctaaataat cagccagcac tctatctttc
     3661 caaatccaca gcatagcaaa gagagcaaaa gagcctgtaa attcagaaat taaggaaacg
     3721 cctcttggtg tacataatct atcttatgtt aaatgaaatc taatttcttg attatatgct
     3781 ggatcgttcg tgggtggctt tgcccctata tttccagaca cctgttatca ttggcccgct
     3841 gccgcagata ttcccgtggc gagcgataat ccagcgcact atgcggatgc cattcgttat
     3901 aatgctcgaa tgcctctgca aggttctttg ctgccgttaa cccgtcaggt ttgggcatga
     3961 tactgatgta gtcacacttt attgttttca cgaagctctt tgctattctg ttactctccg
     4021 gactccgcac cgccgtgttc ttcggttcaa caaagtccca acatccgggt gaactggcgt
     4081 gtttcattag ctcggtagca tgaaccatta tccgtcagtc acttcactgg agacgccgga
     4141 agctcgttgc cgaagcggtg ttccaccgct cccagcatgt cgccaacggg agagcatcta
     4201 cggtctgatt gaactgttta ttcaaattgt ccaccgtctg tcagtacgct ctgaacgtcg
     4261 gctggttaaa acactgctcg ctgattttca gagggttcac ggtaaaacag cacttttatt
     4321 ccggattgcg gaagcggctc tgaataatcc ggatgggctg gttaaagagg ttgtgtacca
     4381 tctgcatatt gttgaaccag atcgcagcgg taagagaagt tcatcatact tggcgcagtt
     4441 acgcgatgtt tctgctagag gtgatgccgt aaagaacggc agaacattac cggaacaaga
     4501 tagcggtcta cctgcactgg tatcagaccc ggggcttccc cggatgatct ccacggtact
     4561 ttaggtactg tgtctatatt atgtaggtag gatcaattaa ctttgtagtt gaaattcttt
     4621 cattgaaata atgaataaaa aagacaaacc gcattaaaca atgccagaat ataattgcat
     4681 ttttgttccg tttttttaaa tcatagtata caaagaagct tgcatactat atgaaaatat
     4741 aacacccttt actatgatgt ttataaaaca atatatggag tcatgtaggt atgaatttag
     4801 atggtgttag accatactgt agaatagtca ataaaaagaa tgaaagcata tcagatattg
     4861 catttgcaca tataataaaa agggtaaaaa attcatcatg tactcaccca aaagcagcat
     4921 tggttttttt aggagagaaa ggtttttgtg atagcaatga tgttctatct attatgggac
     4981 aacaaatacc aagagtattt aagaacaaga tgttatatga ttatgttttt aaaaatgaaa
     5041 aaagtaaaaa tgattttcta aaaatggctg aatcatggct accacagagt gaaccaatag
     5101 taataaataa tgatgatgac gcattgaatg ctgctgctta tttttctgta aaaaaagcga
     5161 aaataaaaac agtaaacgat actgatttta aagagtataa taaggtttat attcttgggc
     5221 acggtagtcc tggttctcat caattaggcc ttggttcgga acttattgat gtacaaacaa
     5281 tcatttcaag aatgaaagac tgtggtattc taaatgtgaa agatatccgt tttacttcat
     5341 gcggctccgc tgataaagtg gctcctaaaa attttaacaa tgcccctgct gaaagtcttt
     5401 cttgtatcct taactctctg ccttttttta aggaaaaaga atctttgcta gagcagataa
     5461 aaaaacacct tgaaaacgat gagtcattga gtgatggtct aaaaatatcc ggctatcatg
     5521 gatatggagt tcactatggt caagagcttt ttccctactc acattatcgt tcaacttcaa
     5581 ttcctgctga tccggagcat acagtaaaaa gaagctctca gaaaaagact tttattatta
     5641 ataaagaact ggattagtat aaaattttta acctatagtt aattgtttgc atttaagatg
     5701 aagttatttt atctttagta gaaaagtgct tccctatgag ggaaaatcac aaatcatcat
     5761 aatacagaga caaaacgata cgaaaaaata gataaaaaac atcattcttt taccacatta
     5821 ttttccgtga atatgaaaaa taatgttatt actttaatat aagactattt tttgtttttc
     5881 catcactctg ttcaaatttt tccgcatgac ttgtgttttt tgtaatacag ctcgtttttt
     5941 acagctgacc aaaatcatca attaattatg ctaaggaaat aaattatgaa aaccaaaaac
     6001 tttcttcttt tttgtattgc tacaaatatg atttttatcc cctcagcaaa tgctctgaag
     6061 gcagaaggtt ttctcactca acaaacttca ccagacagtt tgtcaatact tccgccgcct
     6121 ccggcagaga attcagtagt atttcaggct gacaaagctc attatgaatt cggccgctcg
     6181 ctccgggatg ctaatcgtgt acgtctcgct agcgaagatg catactacga gaattttggt
     6241 cttgcatttt cagatgctta tggcatggat atttcaaggg aaaatacccc aatcttatat
     6301 cagttgttaa cacaagtact acaggatagc catgattacg ccgtgcgtaa cgccaaagaa
     6361 tattataaaa gagttcgtcc attcgttatt tataaagacg caacctgtac acctgataaa
     6421 gatgagaaaa tggctatcac tggctcttat ccctctggtc atgcatcctt tggttgggca
     6481 gtagcactga tacttgcgga gattaatcct caacgtaaag cggaaatact tcgacgtgga
     6541 tatgagtttg gagaaagtcg ggtcatctgc ggtgcgcatt ggcaaagcga tgtagaggct
     6601 gggcgtttaa tgggagcatc ggttgttgca gtacttcata atacacctga atttaccaaa
     6661 agccttagcg aagccaaaaa agagtttgaa gaattaaata ctcctaccaa tgaactgacc
     6721 ccataaagct ggacagcctg tatcaggcta tggagggccc atagacaaat ctaccctata
     6781 tgagcatagg aggagtctat gggcacacca cgttttaccc ctgaatttaa gggattactg
     6841 gaaaggctgg gacatatccc tccggcagaa gcagaaaaag cttattatgc tgccatcgga
     6901 aacgatgatc tggcaacctg agttcacaga taaaacattc tctaggaaac tcggggcggt
     6961 tccgttcacc acatgcaatg tggtgttgca ggggaacggt ctgcaccatc ccctatgtcg
     7021 atcaatataa cagaaatgac aacttcagat tcagggcaca acctaaatat attttaggtc
     7081 acctctcaaa tcgtttgcct gataccgctc cattttttaa taaaaaaatc aatcattttt
     7141 gaatctttgc ttttcattgc attgtcaagc atagtgtccc cactattt
//
LOCUS       DAJTRD010000154         7160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTRD010000154 DAJTRD010000000
VERSION     DAJTRD010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.154"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="OO315_003807"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="OO315_003807"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5892545.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
     gene            2269..2742
                     /locus_tag="OO315_003808"
                     /pseudo
     CDS             2269..2742
                     /locus_tag="OO315_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            2907..7025
                     /gene="pic"
                     /locus_tag="OO315_003809"
     CDS             2907..7025
                     /gene="pic"
                     /locus_tag="OO315_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR5892546.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGETVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
BASE COUNT         1901 a         1664 c         1978 g         1617 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcctc ttactctact cagcacatgg
     2281 cagggcttcg tctatgtggc gttcatcatt gatgtgtttg ccggatacat cgtgtggtga
     2341 cgagtctcat cgtctatgga aacgacattc gtgctggatg cactggagca ggcgttgtgg
     2401 gcccgtcgtc cgtctggcac catccatcac agcgataaag gctctcagta tgtgtcactg
     2461 gcctataagg cgcgactaaa agaagcaaaa ctgctggcat caacagggag tactggtgac
     2521 tcgtatgaca acgcgatggc agagatcatc aagggccttt acaaagcgga ggtaatacac
     2581 cgtaagagct ggaaaaaccg gacagaagtg gagctggcca cactcacgtg ggtggactgg
     2641 tataacaatc gacgattgct ggaaaggctg ggccatatcc caccggcaga agcagaaaaa
     2701 gcttattatg cttccatcgg aaatgatgat ctggcagcct gagttcacag ataaaacact
     2761 ctccaggaaa cccggggcgg ttcagttcac aaaaacacat taatgcagta actatatttt
     2821 cctttctggt gataacgtcg ggttatcatt agcttcttca gctattttac ttttatatcc
     2881 cttgtaaaca tcatggagaa tccatagtga ataaagttta ttctcttaaa tattgccccg
     2941 tcaccggggg gcttattgct gtctctgaac ttgcccgcag ggtaataaaa aagacatgcc
     3001 gaagattaac gcatattctt ctggctggca ttccagcaat ctgtctgtgt tactctcaga
     3061 tatcccaggc gggtattgtc cgttccgata ttgcctatca gatttatcgt gatttcgccg
     3121 aaaacaaagg gctttttgta cctggtgcca atgatattcc ggtatatgat aaggacggaa
     3181 aacttgtggg aagactgggt aaagccccaa tggccgattt cagcagtgtg agctcaaatg
     3241 gcgttgctac gcttgtatcg cctcagtata tcgtcagcgt aaagcataac ggaggatatc
     3301 ggagtgtgag ctttggtaat gggaaaaata catattccct tgttgaccgt aataaccacc
     3361 cttctattga cttccatgct ccacgtctga ataaactggt tacagaagtt attccctcag
     3421 cggtaacatc agaaggaacc aaagccaatg cttataaata cactgaacgt tacaccgctt
     3481 tttatcgggt gggtagtggt acgcagtaca ctaaggacaa ggacggaaat ttagttaagg
     3541 ttgccggtgg atatgctttt aaaacaggag gaaccacagg agttcctctg atatctgatg
     3601 caacaatagt ctctaatccc gggcaaactt ataatcctgt aaacggccct ttacctgact
     3661 atggagcccc tggggatagt ggttctcctt tgtttgctta tgataaacaa caaaaaaaat
     3721 gggttattgt tgctgtatta agagcatatg caggtattaa tggtgctacg aactggtgga
     3781 atgtcatacc aacagattat ctgaaccagg ttatgcagga cgatttcgat gcccccgtag
     3841 actttgtttc cggactgggc cccctgaact ggacatacga caaaacatca ggcacaggta
     3901 ccctgagcca gggcagtaaa aactggacca tgcacgggca gaaagacaat gacctcaatg
     3961 ccggtaaaaa tctggtattc agcgggcaga atggtgcaat tatcctgaaa gacagtgtga
     4021 ctcagggtgc cggttatctc gaatttaaag acagttacac cgtatctgct gaatccggaa
     4081 aaacatggac gggtgccggc attattactg acaaggggac gaatgtaacc tggaaggtca
     4141 acggcgttgc cggtgacaac ttgcataagc tgggggaagg aaccctgacc ataaacggaa
     4201 caggtgtaaa cccgggagga ctgaaaacgg gagacggtat cgttgtactt aaccagcagg
     4261 cagacactgc aggtaatatc caggccttca gttcagtgaa cctcgccagc ggacgtccga
     4321 ccgtggtgct cggggatgcc cgtcaggtca atccggataa catttcatgg ggataccggg
     4381 gaggtaagct tgaccttaat ggtaatgccg ttaccttcac ccgactgcag gctgctgatt
     4441 acggggcggt gattacaaat aatgcacagc aaaaatccca gcttttactg gatcttaagg
     4501 ctcaggatac aaatgtcagt gaaccgacga ttggaaatat atcccccttt ggtggtaccg
     4561 gcactccagg aaacctgtac agcatgatac tcaacagcca gacccgcttc tatattctga
     4621 aatctgccag ctatggtaac actctgtggg ggaacagcct gaatgatccg gctcagtggg
     4681 agtttgttgg catgaacaaa aacaaagcag ttcagacagt aaaagatagg atcctggccg
     4741 ggcgggcaaa acaacccgtt atctttcatg gtcagctgac cgggaatatg gatgtcgcca
     4801 ttccacaggt gccgggggga agaaaggtaa tctttgatgg tagcgtgaac ctgccggaag
     4861 gtaccctgag tcaggacagt ggcaccctga tattccaggg acatccggtt atccatgcct
     4921 ccatcagtgg cagtgcaccg gtcagcctga accagaaaga ctgggaaaac cgtcagttta
     4981 caatgaaaac actgtcgctg aaagacgctg acttccatct ttcacgtaac gcctcgctga
     5041 acagtgacat taagtcggat aacagccata tcacactggg aagtgacagg gcatttgtgg
     5101 ataaaaatga cggaacagga aattatgtca ttccggagga aggtacctct gtcccggaca
     5161 ccgtgaatga caggagccag tatgaaggga atattacgct gaaccataac tcagccctgg
     5221 atatcggcag caggttcacc ggggggattg acgcttatga cagtgccgtc agcatcacct
     5281 ctccggacgt cctgttgaca gccccgggtg cttttgccgg cagttcactg acagtgcatg
     5341 atggcggtca tcttacagca ctgaacggtc ttttcagcga cgggcatatt caggccggta
     5401 agaacggcaa aatcaccctg agcggtacac cggttaaaga tacggctaat cagtatgccc
     5461 ctgctgtata tctgacggac ggatatgacc tgaccggcga taacgcagca ctggaaatta
     5521 cccgtggagc acatgcttcc ggtgatattc atgcctctgc ggcatcaaca gttaccatcg
     5581 ggtctgacac gccggcagaa ctggcttctg cggaaacggc tgcatcggcg tttgccggca
     5641 gtcttcttga gggctataac gcagcattca atggtgccat aaccggtggc agggctgatg
     5701 tcagtatgca taatgcactg tggactctgg gtggggactc tgccatccac agtcttaccg
     5761 tcagaaacag ccgtattagt tctgaaggag accgtacatt ccgtaccctg acggtgaata
     5821 aactggatgc aacaggcagt gattttgttt tgcgtacgga cctgaaaaat gccgataaaa
     5881 ttaatgtgac tgaaaaagcc actggttcag ataacagcct gaacgtcagc tttatgaata
     5941 atcctgctca gggacaggcc ctgaatattc ctctggtcac ggcaccggcg ggaacttcag
     6001 cagagatgtt taaggccggc acccgggtga caggtttcag tcgggtgacc ccaaccctgc
     6061 atgttgatac cagtggtggc aatacgaagt ggatactgga tggttttaaa gcggaggctg
     6121 ataaagccgc tgccgcgaag gctgacagtt tcatgaatgc cgggtataaa aacttcatga
     6181 cggaagttaa caatctgaac aaacgtatgg gtgacctgcg tgacacaaac ggtgatgccg
     6241 gtgcctgggc gcgcatcatg agtggtgccg gttctgcaga cggtggttac agtgataatt
     6301 acacccatgt tcaggtcggc tttgacaaaa aacatgaact ggacggtgtg gacctgttta
     6361 ccggtgtcac gatgacctat accgacagca gtgcagacag ccatgcattc agcggaaaga
     6421 cgaaatcggt ggggggcggt ctgtatgctt cagcattgtt tgagtccggt gcctatatcg
     6481 atttgattgg taaatatatt caccatgaca atgattacac aggtaacttt gctagcctgg
     6541 gaacgaaaca ctacaacacc cattcctggt atgccggtgc tgaaacgggt taccgctatc
     6601 acctgacaga ggacacgttc attgagccgc aggctgaact ggtttacggc gccgtgtccg
     6661 ggaaaacatt ccgctggaaa gacggtgata tggacctgag catgaagaac agggacttca
     6721 gtccgctggt tggaagaaca ggggttgaac tgggcaagac cttcagtggt aaggactgga
     6781 gtgtgacggc ccgtgccgga accagctggc agtttgacct gctgaataat ggagagaccg
     6841 tactgcgtga tgcgtccggg gagaaacgga taaaaggaga gaaggacagc cggatgctgt
     6901 ttaatgttgg tatgaatgcg cagataaagg acaatatgcg ctttggtctg gagtttgaga
     6961 agtcagcctt tggtaaatat aacgtggata atgcggtaaa cgcgaatttc cggtatatgt
     7021 tctgatgtgg gggtaaaggg ctgccggaag gcagcctttc tcagagactc ttaaaatata
     7081 aaactgaagg tcttcggtaa gggggagtgt attagacgtc aactagtttt gccgactggc
     7141 gcgagcctgt ttaagattct
//
LOCUS       DAJTRD010000155         7083 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTRD010000155 DAJTRD010000000
VERSION     DAJTRD010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7083
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.155"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(296..1198)
                     /locus_tag="OO315_003810"
     CDS             complement(296..1198)
                     /locus_tag="OO315_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR5892547.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO315_003811"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO315_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5892548.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="OO315_003812"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="OO315_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5892549.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="OO315_003813"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="OO315_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5892550.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="OO315_003814"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="OO315_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5892551.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="OO315_003815"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="OO315_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
BASE COUNT         1644 a         1793 c         1855 g         1791 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gag
//
LOCUS       DAJTRD010000156         6923 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTRD010000156 DAJTRD010000000
VERSION     DAJTRD010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.156"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..695
                     /locus_tag="OO315_003816"
     CDS             <1..695
                     /locus_tag="OO315_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5892552.1"
                     /translation="DAEAICEAASRPSMRFVQPRTETQQAIRALHRVRESLIRDKVKT
                     TNQIHGFLLEFGISLPTGDAVIKRLSLVLAEHEIPEYLSRLLVRLHTHYLYLVEQIAE
                     LESELSQSINADDTAQRIMTIPGVGPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQ
                     YSTGGKSTLLGISKRGDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVV
                     ACALANKLARIA"
     gene            complement(1034..1804)
                     /locus_tag="OO315_003817"
     CDS             complement(1034..1804)
                     /locus_tag="OO315_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892553.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1849..2283)
                     /locus_tag="OO315_003818"
                     /pseudo
     CDS             complement(1849..2283)
                     /locus_tag="OO315_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(2295..2516)
                     /locus_tag="OO315_003819"
     CDS             complement(2295..2516)
                     /locus_tag="OO315_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892554.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2517..3200)
                     /locus_tag="OO315_003820"
     CDS             complement(2517..3200)
                     /locus_tag="OO315_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5892555.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(3264..3575)
                     /locus_tag="OO315_003821"
     CDS             complement(3264..3575)
                     /locus_tag="OO315_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892556.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3572..4474)
                     /locus_tag="OO315_003822"
     CDS             complement(3572..4474)
                     /locus_tag="OO315_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5892557.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4914..5873
                     /locus_tag="OO315_003823"
     CDS             4914..5873
                     /locus_tag="OO315_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5892558.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5873..6268
                     /locus_tag="OO315_003824"
     CDS             5873..6268
                     /locus_tag="OO315_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5892559.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6451..6831
                     /locus_tag="OO315_003825"
     CDS             6451..6831
                     /locus_tag="OO315_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5892560.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
BASE COUNT         1892 a         1791 c         1676 g         1564 t
ORIGIN      
        1 ttgatgcaga agccatatgt gaagcggctt cacgaccatc aatgcgtttt gtccagccaa
       61 gaactgagac ccagcaagca ataagggcat tacaccgggt cagagaatcg ctcatcagag
      121 ataaggtgaa aacaaccaac cagatccacg gtttcctgct ggaatttgga atcagcctgc
      181 cgacagggga tgccgtaata aaacggttgt ctctcgtatt ggctgaacac gaaatccccg
      241 aatatctgag ccgccttttg gtgaggttgc atacccacta cctttacctt gttgagcaaa
      301 tagcagagct ggaatcagaa ttaagtcagt caatcaacgc tgatgatacc gctcagcgca
      361 ttatgacaat tcctggtgtt gggccgatta ctgccagctt actatcgtcg caacttggtg
      421 acgggaaaca gttttcatgc agcagagatt ttgcggcttc cacagggctc gttccccggc
      481 agtatagcac tggaggaaaa tcgactctgt tgggtatcag taagcgaggt gataaaaatt
      541 tacgccggtt actggttcag tgtgcccgct cattcatgat gcagcttgaa cgccagcatg
      601 gaaaacttgc tgagtgggtt cgggagcaac tcaacaagaa gcattcgaat gtggttgcct
      661 gcgctctggc aaataaactg gcccgaattg cctaggcaat cactactcag caaaaagaat
      721 atcaggcctg atatatagat gctgacaaaa ctcaattagc ttattgcaaa caataagata
      781 caagttaccg tctggttttg cgaagattga ttattgatga catgaacggc aaatcgacct
      841 gattaaaaac ctaacagggg aaaatggctc actgaagccg aggggatttt taggattatc
      901 aggtgcggaa ctcatcgagg cgcgggcatc ccttgcccac agagacgccg gatagattta
      961 agcaagccca ccattaatca aaaatcagtg ttgcaaaaac gggggtgacc atagattttc
     1021 cccgccatcg tcatcatttc cggatttttg ccagggcatc cgccatgctg cggatttcgg
     1081 cccgggcttc ccggacaact tcccgcagaa caccacggtc aaaccaccgg cggggcggtt
     1141 catccggtat acgcagcaca tcgcaggcca gcccactgga caggatatgc tcaatacaat
     1201 cgctgaccag atacatttcc accgccgaaa aacgtgcgcc ccggttcagg atggcatcga
     1261 gcaccacggg caggttttca tcgtcttcat cgagccagca ggggtgatcg ctttccttca
     1321 cgaccaggcg aaagcggcgc gtctggcggg tcagcaacgt ctgaccgctt gtggtcagcg
     1381 ccgcccggat acgccgcgca tcttctgccc cccggcaact gacactgcat actgcatcgc
     1441 gcaccgcatc aagaactgcc ctgatttcgt cttcggtcag ccattcaccg gcatacggga
     1501 aagacacctg cgttccgtcc gccggaagta ccggcagcga ctgccactga ctgccggaca
     1561 gaacaagttc cgctccggtt ccggtcagtt tctgaatggc ggaggccagg tgcggcggca
     1621 gcacgccatc gtggcggtta acgagtaacg tcgccagccg gggcgcatcc agccgcacgt
     1681 aaatgctgat ccagcccgga tagttcgggt tacattggtc aacatccgtc tcttccggcc
     1741 agccctgcgc cagcagcagg ttacgggcgt gacgacaaat cagagcattc agcgtttcat
     1801 tcataattca tgaactccct gtatcgccgc ccacaggcgg cgataacatc agtcgttttc
     1861 gtgtgtgttg tttttcagca ggctgcgggc ctgatcaacc atcgcctgtc ggcgggtaac
     1921 gtattccggg agggggaaac cgtgatcccg ggcagcccat tcacgtttcc acagtgcctg
     1981 ttcagcgatc tgagtcaggg tctccagcag ttcagcaggg acggtcacct ggcggttttc
     2041 gcgcacgttg ctgataatct ccatcgccgc ggcggaagaa ataccggact caatccgcta
     2101 gtcttccggg atgtcctcca ggtgcgccag gacggtcctg atttcatgat cggacaggga
     2161 aatatccatt ccgtcagcga gggcctgaat ctcttccggg gaccagataa ccagcatcag
     2221 cggcgtgtct cccgaatact ttgctgccag cgcccggcat agcgtttcgc atgttccgta
     2281 catactctct cccctcaatc tttgaagcag gaatccagtt ccagaatttc ggtatatgcc
     2341 tccatcaaga ggcggtttgc gcgaaacaac gtctgacgca ccagacgttc cggcagggtg
     2401 tcagcattaa agctgtaccg ccgctccatc tcattcatgg ttgtacgcag gtcgcacatg
     2461 gcattggcca cctccgccac ttcggcgcga agtttttcgt gtccggcata gttcatttac
     2521 gcagcctccg gcataaacca gtcatcattc gcggccccct gctgcatggc ccgctgtacg
     2581 gcagccagtc gttgctgtcc ggcacggtga tactgctcaa gcagctcgat accgatatac
     2641 cggcgtccgg actggagggc ggcgacgcag gttgaaccgc tgcctgcaaa cgggtccagc
     2701 actatcgcat tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt
     2761 tccgtcgggt gatggcggtt gcccgaatat ttccagccca acacgtccgg cagcgggttt
     2821 tgtggcagac gtggacggcc ttttgccagg atgtaggcgc attcgtggcg atagcccaca
     2881 tatgcggcct tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc
     2941 gcatttttcc aggcagacat aaagcgatcg acgcggttcc agccatagaa gctcaccatc
     3001 agcgcgtctt ttttgagtac gcggtacatt tcattacagg ccggttgcag ccattcatca
     3061 gttttatcac cagcaatggt acgcccctga cggtcacgaa aaccgacgag atatggcgga
     3121 tcggtgagga tgaaatcaat ggcgttatca ggaatacggg ccataacatc gatgcagtta
     3181 ccgaggacaa aacgggacat aacagatcct tctcttacag agggcacccc tgtggtgccc
     3241 gttccccgct gcctgtgggc tgttcatcgc ttccggtaac gggtcaggcc gaccgtggaa
     3301 taccgcagcc gtcgtctttt tgacagcaag gttatgccgc gaaagcggct tgaagggcgt
     3361 tcatcagaaa agggcgacgg gctggcgtac actgtcaccg acaccagccg ggagcggtaa
     3421 aagcgcccgt cccgtgcccg ccggacgcgg gacactgacc acaggcagcg tttatgccgc
     3481 gccgcgtcag ccggcgcagt aaaaagagga gcacggcgac atcctttgtc ggaaaagaaa
     3541 cgggcggttg cccgcccgtc tccgtcatac ttcatccgcc ataatgcgcc acattgtcga
     3601 ctatccacgc aggtccggtg acttccggct gctcatcgtc atctgtcggg gaagaccatt
     3661 caagttcccc ccacagcaca ttgacgagct ctccacgctc atacaactgc cagccacaga
     3721 aatcacagcc ctgttcggca taccagtgct ccagcgtgca gctgaaacgg cggcttaatt
     3781 ctgcaataac gtcgctttcc ggctgacacc acggagtgtc gaaatccacc tgaatgaagt
     3841 tttctccctg actgctgata ttcaggtcat atccacaagg ccacttcacg ccataccgtt
     3901 ccgtatacca gtgatatgca gaaggaacac cgttcagaac accaccatta aagccgttca
     3961 cttcaacatc caggcgggtc ggcagaaccg tcagcaggtc aaacggcatg gttttttccg
     4021 gcactacgtt gtcacacaga cggttccacc acaccgatac atcctcgtta ccccatatgt
     4081 cacaccagtc acctcttttt gcggtgaaat gcacagtgat ggtttccctg gcatcatccg
     4141 gtaatccttc ccaacgacgc tggccagtac cgctctgtaa ccagagttca tgcagcatca
     4201 ggcagttttg ttcatccagc aacacaccgt tctgcaggtg tgtcagccag cgggtgaacg
     4261 cgatattttc cgtcgataca gcaccacgtc cggcagccgt cactccgggg cactgttcag
     4321 agcggatatt ttcagtgatc tgcagaagtc cggcacttcc ggccagaaac agctgaatac
     4381 cttcatttgt ggcgcggcga taaagcggtg tgactgcacc gctggccagt cgtttaatct
     4441 cagcgatctg tgccggctca ccaggaaaat acatacgatt cgagcaccag tttggcatgg
     4501 gaattctcct ctcacatgcg gcctccatca ggccgtgccg cacctgtaac cgggcgaccg
     4561 catacgaatg caggtgcggc cccgaagccg cagcgcgtgg aggaggaatg acgaaacgtg
     4621 accgggtgag gaggccggcg acagcgacac acttgcatcg gatgcagccc ggttaacgtg
     4681 ccggcacggc ctggttaaat agtatccgtc tcagttcgtg gcgcaaaatg agaaaccaat
     4741 aagaactcaa aatgaaccac aacaagaaca taaagagact ctaaaagagc aacaccgttt
     4801 gacatagact caaaatgagt cacaataaga aacaataaaa aactcaaaaa gatgcaccga
     4861 cataaaggat gctcaatttg agataaacac aaactcaaaa cgaggcaaaa agaatgctaa
     4921 aagtatcatg cgatgatggt tcaactaacg taaaactggc ctggcttgaa gatggcgagg
     4981 taagaaccag tctgtcagga aacagcttta aagaaggctg gaacccggga ctgtttaatg
     5041 ccggcaaagt ttacaattac gtcgtggatg aaaaaaaata cacctatgat ttgggaagca
     5101 ctgctgtcat aggtacaaca catgtcagtt atcaatacag caccaccaat ctgcttgcca
     5161 tacatcatgc actgctgaca tccggccttc agccacagga tgtagaactg acagtaacat
     5221 tacctgtgac cgaattcttt gataatgaca atcaaccaaa tgaagaaaga atagaaagaa
     5281 aaaaggcaaa tgttctccgt gagatatctc tgaataaagg ggaaacattt aaaattaaaa
     5341 aagttaatgt aatgcctgaa tctttgccag ctgcatttga atcactaaaa aaagataaag
     5401 taaacaaact tgaacgcagc ctgattatcg atcttggagg aaccactctg gattgtggtt
     5461 taattctcgg tgcatttgaa ggcatttctg aaatccgtgg ctattcagaa atcgggacat
     5521 cccgcatcac tcataccgtc atgaatgcgc tgacgaaagc ctccacacca tgtaattatt
     5581 ttattgctga tgagttaata aaaaacagac acgataatga atatctccag acattaataa
     5641 atgatgtagc tgaaataaaa aacatttctc atgtaataga cagagaagtg aaatctctgg
     5701 cagaaagcat caggcaggaa atttcaacat tctcaggaat gaaccgaatc tatctcactg
     5761 gcggaggagc cgagcttatt taccctcaca taaagcaata tttcccgaat ctgaaagtga
     5821 acaaggtgga tgaacctcag tttgcattgg tgaaagcaat ggtgcatgcg taatggaaag
     5881 cagtgatcca aaaaaaagaa agaaagttgt ggcatacctg cacccggcac tttatccgca
     5941 ggacaacctg acacaacaaa caatagactc tctcccagta caaatgcggg gagatttcta
     6001 cagacaatcc cttatttgcg gagcagctct ttattctgtt gccccccgac tgttaacgct
     6061 aatcagcgtt tttttcagtg agaaaatcac tgcagaaaat ctggtgaaac ttatagaaca
     6121 aacaacagga tacacatcca catcaattga tataagtgtg ttaaaaaaca tcatagaggc
     6181 atcttcagaa aacaaatcag agagcatcac ttcaaaagat gatttcgaag aacaaaccag
     6241 acgcaatctt tctatgttaa aaaaataata aaagaagagc aggttcagtc aattacctgc
     6301 tcttctttta tacatactaa aaaatcagat ttttacgaat attcagatta tcataaaaaa
     6361 tgcaacagac aggaatgcaa taattttgca ttatctcgtt gcttgtaatg caaatatgca
     6421 ttattgtcag tgtgtgacaa taacagagag atgacgatgg cgaccattaa cgcacgtatt
     6481 gatgatgata taaagaacca agcagatgaa gtgctgaagc tgatgaatat cagtcaaact
     6541 caggctattg cagcttttta tcagtatatt actgaacaaa agaaactgcc atttgtgatc
     6601 acctcaattg taaaaacgcc gcatgattta ttacgtgaaa gtacagacat gttagctgaa
     6661 gctcttgcag tgatttccaa tctacaggtg tggactgaac agcaggatgg gattggaaaa
     6721 gcaaagctaa tggagtatta ccgccgcctc gacgcactgt actgttgtgc aaaagagaaa
     6781 ataggtcttc tttcagataa ccgggatgct gaactaggct gtgtccctta atcatctgag
     6841 ttatagtaac tgtctgttta cacaacatat tgaactatgg ctcgctacga ccttcccgat
     6901 gaggcatgga ccatcatcaa gcc
//
LOCUS       DAJTRD010000157         6812 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTRD010000157 DAJTRD010000000
VERSION     DAJTRD010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.157"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO315_003826"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO315_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5892561.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO315_003827"
     CDS             2089..2238
                     /locus_tag="OO315_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892562.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3027..3467)
                     /locus_tag="OO315_003828"
     CDS             complement(3027..3467)
                     /locus_tag="OO315_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5892563.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3685..4074
                     /locus_tag="OO315_003829"
     CDS             3685..4074
                     /locus_tag="OO315_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892564.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4118..4522
                     /locus_tag="OO315_003830"
                     /pseudo
     CDS             4118..4522
                     /locus_tag="OO315_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4629..5465)
                     /locus_tag="OO315_003831"
     CDS             complement(4629..5465)
                     /locus_tag="OO315_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5892565.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5465..5740)
                     /locus_tag="OO315_003832"
                     /pseudo
     CDS             complement(5465..5740)
                     /locus_tag="OO315_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5815..6288)
                     /locus_tag="OO315_003833"
     CDS             complement(5815..6288)
                     /locus_tag="OO315_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR5892566.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6309..6812)
                     /locus_tag="OO315_003834"
                     /pseudo
     CDS             complement(6309..6812)
                     /locus_tag="OO315_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1591 a         1763 c         1772 g         1686 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtgattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc acttatacat
     2641 cacttttaca tcattccttc agctcatacg tcactttcat cctctgtgcc atttgtacat
     2701 cacccccttc ctgaacgtgc tgacagcggg ttttccggct gcgccggcaa cgcttcacgg
     2761 ccacgcaata aattgcgtcc ccgttcaccg ttttcggatg ggaagaatgc cggaaacagg
     2821 acttaccccg gctgaatggt gtgaatttcc gcgctatgca cttgtgcgca tactcatgca
     2881 tgccgtaaaa acagagcctg cgcgtttctg gcgggttttc gggtggtttg ttgcctgttt
     2941 taccggtttc ccgtcagaaa cgccctgagg gcctctcagg cggtgctgat aacagatgtt
     3001 atggtaaatc agagtcacct gcactgttac tctgaaccgg agcgaaacag ccgcgaccag
     3061 aatccccgct ttttttcctg tgccagcgcg tcagccagac ggctaatttc ggcctgtagt
     3121 tgttccctct cctcatttcg caggcggttt tctgcttccc gcgccatctt gtcttcaagc
     3181 attagggtaa cagtctgctg cagagcgtcc agtttctgct gaatgacgtc tagctgagca
     3241 ttttccgcct ggctaacgac agatttggag tgtccctcct tgtgcctttt tggtgtctca
     3301 gaagtgcgca gctcgccata ggcccgaatc agttcactgg tctcgaactc ccggcgatca
     3361 ttttttcctg tgcgatagct gacaaggcct ttagccatgt cacgatataa ttgcgagcgc
     3421 gactttcctg ttaatttcag tgtctcttga actgtgtgcc atgccataaa agtgacagtg
     3481 tctcctgtgt gtaccgtatt gtaacagcag aacatattac tgttttaaca caaaaggtct
     3541 gaggctgccc gtgaggcgtg aaaaatacac aaaaaacctg ttgacggtgt ggttttgacg
     3601 ggcgtagtat ttcgaatact gtatataaac acattgttat aaatacagtt tttgctgttt
     3661 ttaaaagtca aagaggataa ttttatgagt acagagttaa atagcggtac tgtcggtggt
     3721 tttggtcaca tgggaccaaa tgacccctac aatgatggtc atagcggctc tggaagttcg
     3781 ggaggtagtt ttaacggtgg tggtaatggt ggccatgata atagtagtgg ttcatcttat
     3841 actgcatcct ttggtctcat cactggccga cacccgataa ctgtcccagg tacgaagaat
     3901 attaattctg tcgatctttc tgctgtcatt aataacatta tacatcatga aggtgcacgc
     3961 aataacatgt acctagatac tgcgaacaat gtaagagtgg gagtgacggg cactggctgg
     4021 caatgtctag caacggcagg catttcggct gagggtaaaa gaactttccg ctaagcgata
     4081 gactgtatgt aaacacagta ttgcaaggac gcggaacatg cctcatgtgg cggccaggac
     4141 ggccagccgg gatcgggata ctggtcgtta ccagagccac cgacccgagc aaacccttct
     4201 ctatcagatc gttgacgagt attacccggc attcgctgcg cttatggcag agcagggaaa
     4261 ggaattgccg ggctatgtgc aacgggaatt tgaagaattt ctccaatgcg ggcggctgga
     4321 gcatggcttt ctacgggttc gctgcgagtc ttgccacgcc gagcacctgg tcgctttcag
     4381 ctgtaagcgt cgcggtttct gcccgagctg tggggcgcgg cggatggccg aaagtgccgc
     4441 cttgctggtt gatgaagtac tgcctgaaca acccatgcgt cagtgggtgt tgagcttccc
     4501 gtttcagctg cgtttcctgt ttggggtcgt ttgcgggaag gggcggaatc ctacgctaag
     4561 gctttggcca gcgatattct ccggtgagat tgatgtgttc ccaggggata ggagaagtcg
     4621 cttgatatct agtatgacgt ctgtcgcacc tgcttgatcg cggccgcgat agctagatcg
     4681 cgttgctcct cttctccatc cgcgttccaa gctgcggaaa ggcacccata agcgtacgcc
     4741 tggtcgagca ggcgacgcgg atcgacgtcc agcgcacgag agaatgcgtc cgccatctgt
     4801 gcaatgcgtc taggatcgag acaaaggtcg tctctgtcag ccggatcgta gaacatattg
     4861 gcggcgccaa agcccacttc accgaccaga ccgacgggat ctatcaccag ccagccgcga
     4921 ctggagaaca tgatgttttc atgatgcaga tcgccatgta gcccacgcag ttccgaggca
     4981 ttgctcatca tttgatcggc tataatcgcc gcgtggacgt agtcagtttg acaacctgcg
     5041 ttttgatcat cgcgcgcccg ctgaaacaaa gctgcaaagc gatcccggat cgggagaagg
     5101 gcagaaggca ggggttcctc agatgcggca tacagcttcg ccattagttc cgctgcaatt
     5161 tcggtcgcct ggtagtcgcc gtgctcggca acgatgtgag agagcattcg ctccccggca
     5221 tattcgagca acatcagatt gttctcacga ccgagcaacc ggactgctcc cctcccattg
     5281 cgccatacca gatagtcggc cccgcgcagt tcatcagcaa tgtcttctat aggtttcaat
     5341 cccttgacga ttgcaggagt cccgtctggc aatgaaactt tccaaacgag gctggaaaag
     5401 gtgtccgcaa tgagaacagg ttgcgaaacg tgccaatgag caggaaaaac aggcggcatg
     5461 aacatcaacc ccaagtcaga gggtccaatc gcagatagaa ggcaaggcgt tcgcggtcgg
     5521 gggcttcgat ccccaataca ttgaatagga cagcgaaggc gcgctctgct tcatctggcg
     5581 ctgcccagtt ctcttcggcg ttagcaatca tgagtgccaa atcggcatag cgatctgctg
     5641 ttccgagccg cccaaggtcg atcagacccg tgcattgaag agttttaggg tccaccatga
     5701 agttcggcat gcagggatca ccatggcaaa caaccatatc gtcgctatca atcgcggaaa
     5761 aaggcgtagt gcgcgaagcg aacgaagctt tttgccgtcg attgcatagc tttgttaacc
     5821 ctttttccaa atttgatagc aatagttaat gtttgaagta aagtgttgct caaaaacaac
     5881 ttcgaaggta tttggaatac tcgggaagaa aacatccccc tctggctcga tgtcgatcgt
     5941 cgataagtgg agcgtagagg ccatgggtaa tgtttctcgg taaatttctc cgccaccaga
     6001 cactataacg tgaccggtga attcagctag cctgtccatg gcctcttcga ttgactgaaa
     6061 tacaactaca ttgtcatcat ttgatgtcca acctgagcgg gtaacgaccg cgtatttcct
     6121 attggggagt gcgcccatag attcaaacgt cttgcgaccc accagaagcc actgattgta
     6181 ggtcaatgct ttaaaaagta gctgctcccc tttcgcggac cagggtatgt ctggaccgca
     6241 accaatcacg ccgtttttcg ctttcgcagc tatcaatgat actttcaagg ttcttatcct
     6301 gggttaacgg tgcgctcttg gtcgagccgc accggtagct ctcgttcgac acgagccaaa
     6361 agatcgagct gcggcgtact cttgtcctcg tccggtaaga agtcgggatt gacggcattg
     6421 cgggacacca catcaacggc gcgtccgaac attcgcgaca gcctgcgctc aaacggacat
     6481 tgatcaaccg ataggctgtg aacagcgcca agttgctgcc ccattgacgg ccacgctttg
     6541 agcaaatccg ctccagacag atcagccgcc ggtactcccg gaattgccgt tatcaccaag
     6601 catgcaccct cctgttcctc ctgccagttg atgacctcgg ggcaagccac acctcgacct
     6661 ttgagccaaa tgaggcggtc acgctctcca gcgagctcac cgcggcggga agcaggtgcg
     6721 attttcgcga aggcatgccc gtcaccacgt cgaaaaacaa aatcaccaga ttctccgcct
     6781 ctgacaggca accagtcaga atgcgattca cc
//
LOCUS       DAJTRD010000158         6795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTRD010000158 DAJTRD010000000
VERSION     DAJTRD010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.158"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            240..2672
                     /locus_tag="OO315_003835"
     CDS             240..2672
                     /locus_tag="OO315_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5892567.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="OO315_003836"
     CDS             2739..4208
                     /locus_tag="OO315_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5892568.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="OO315_003837"
     CDS             4208..5869
                     /locus_tag="OO315_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5892569.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="OO315_003838"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="OO315_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5892570.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6795)
                     /locus_tag="OO315_003839"
     CDS             complement(6478..>6795)
                     /locus_tag="OO315_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892571.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1612 c         1554 g         1626 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataa
//
LOCUS       DAJTRD010000159         6737 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTRD010000159 DAJTRD010000000
VERSION     DAJTRD010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6737
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.159"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(386..820)
                     /locus_tag="OO315_003840"
     CDS             complement(386..820)
                     /locus_tag="OO315_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR5892572.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(808..1209)
                     /locus_tag="OO315_003841"
     CDS             complement(808..1209)
                     /locus_tag="OO315_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892573.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(1469..2038)
                     /locus_tag="OO315_003842"
     CDS             complement(1469..2038)
                     /locus_tag="OO315_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR5892574.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(2783..2926)
                     /locus_tag="OO315_003843"
                     /pseudo
     CDS             complement(2783..2926)
                     /locus_tag="OO315_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(3260..3526)
                     /locus_tag="OO315_003844"
     CDS             complement(3260..3526)
                     /locus_tag="OO315_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892575.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            3667..3873
                     /locus_tag="OO315_003845"
     CDS             3667..3873
                     /locus_tag="OO315_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5892576.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            3968..4570
                     /locus_tag="OO315_003846"
     CDS             3968..4570
                     /locus_tag="OO315_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892577.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNINYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            5770..6687
                     /locus_tag="OO315_003847"
     CDS             5770..6687
                     /locus_tag="OO315_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892578.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYRHSR"
BASE COUNT         1663 a         1759 c         1587 g         1728 t
ORIGIN      
        1 ttcgcctgcc gggagtcaca tttttctgca tatctgacac tttggttccc actacttatt
       61 tggtggacac cactttgtct aattcgtcag attctgacca gacggttcag gctgtacgct
      121 taccactatt ttatccctgt tgaatttggt taagccggtg cagacgcgcc gggaaacgca
      181 cacctgaacc ggcacaataa aatacaatac aaccccctgt tactggagat acgaatggaa
      241 tgacgaggca gcctgtcagt cacagggggc tgttggcata aaatcaacgg aaaggccacc
      301 aacaccggaa ttaagctcca cagtccccgc actggcaatc cattcccggg aaaggcatca
      361 ccgcaaactg ctccggaggc aaccattaca gcctctgtaa cggattatca gccctggcat
      421 cttccgacgg cacctcctgc cgcccccacg ccctgctgta taccccggta ccgcagcgtg
      481 ggcagcactt ttccccctca taatcttcgt cacacatcac acaaaaccac tttctcacct
      541 tcaccgtata atccggaacc gtcatgccct gcagatgcaa acgacgggcc tctttctctg
      601 ccgggtcaag ccaggtacat cgcagcagga ttttttcagc gacagtcatc tggtcatgtt
      661 caaaccacgg tggttgtccg tcattgcccc gctgaagtgt taacgggcat ccacaggaca
      721 cacagcacaa aacgtcctgt cggttgtgct tcgcagcttc tttcgccgta acaaaatggc
      781 ctttgctgtc attcgccata taacagttca gaattcgcat atttcctcct gagttgtccg
      841 gggaatactg aatcctccgg tctggcagtg tgtgcagtac tgctccccat aataatcgtg
      901 gtgacattgt ctgcagtgcc agctggcttt acgcaccacg ggtaaggcat ccggtacgaa
      961 ttgctgcaga cgtttaatca actgtatttc tctgcgctcc ggcctgacat aagggcactg
     1021 ttgaccgtgc tctgtcagcc tgtcgtcagt gtgttcaaac cagggaagtt cagtttcgta
     1081 ttgcggatgg tatctgagtg cactgccaca caagtggcag gtgtagttag cataaggtgc
     1141 ggcctgtgca gtacgggcgc ccgtcagacg tccgttgcca tcaagagcga taaaggattt
     1201 tgcgtacata gtcatatacc tcagccgcca gacgacacgc aggcaacagc cgtcccggta
     1261 cgggcagcgt ggtcagggtg tgaatggcgg ttcagttaaa atctggactg gagtttgggt
     1321 aaagcaggct ggttgctgtt acagcactgt ggttgtcaca caatggaaca ctgacgtaaa
     1381 aaacagtcag tgggcgagtg gatacatcgt tgccgccaga caacaaccca tacttttggt
     1441 ttgccggata ttggcgcgcc agtcgtgctc aactccggct acagccaaaa ttgcgctcac
     1501 cggtgcagtg agctttggta tgctccttcg ccagatagtc agcacgttcc agcacttgct
     1561 gaaagccggt gtcatcatta cgcgccagcc acaccgccgg ccaggccgga aaatgcacca
     1621 gcgtggcatg gcacccgaca tccactccca gggcactgca ccacgcctgt ttaatcatcc
     1681 cggccagaga ctccggagcg cggtaatcac cggcacgaca ccaggtatcc cggttgacca
     1741 gcagcatcag gtgatagtgt tttttacctt tgcactcccc aaactcccgg gcccagacgt
     1801 aatgcagggt tgtgggatgc acgcgtttac cttcccgatg cttacgtttc tggtaagcgt
     1861 cgattcgggc tttcagggca ttgatgaagc gggatatcac agctgcgtcc gttgctgccg
     1921 gtacatccgg gagacgcagg tcaacccgca atgccgtcag gcggggatga acattcagtg
     1981 cgtgccgcac cgtctcacga atacgttgct gccagaaggg gttgtatttg taggtcatgg
     2041 ttaaatctcc gtatggttca tacggaatag ccacgtcgta aaaaatacgc agagtccctg
     2101 aagtgacctc cggcaggaca ctgtctcagg cactctgtga taacccgaac ggtcgtctcc
     2161 ggactgacgg ttgaattttc agcgtcgttt tttaatgtaa aaaaccggtt acgggtaagg
     2221 ctgtgaggag ggagtgatgg tgatacgcaa aaagaagtgc agggactgtg gaaacgcgat
     2281 tactcataat acagtgtgtt gcccgtattg tggttctgtc gaccccttcg gctactaccg
     2341 gaatacagac cgtatcgtta ccatactgct ggcgctgatt attgtggtcc tgctgactac
     2401 tgtcagcgtc agtgtgtgta tactgtgtag ctggtgagat aacggttcat gtatcccctg
     2461 agctgacagt gtaccgtgta tattaagcgt aaccgacagg ttctccttca taccatatta
     2521 aattttccag ggattgccgc tgccttattt cgtcccatac aggaaaactc atctgggttt
     2581 ctgacaaata cgcgaaactc acagcccctc aatatcaacc acacgttgta agccggtaat
     2641 acctgaaaat cagcctatta ccgcccacgc ctccggcacc attccctttt gatttcgaat
     2701 tctttgttgt cgctataccg cttaatgttc ccggtcctcc ggtgcagata acaccggagt
     2761 catcgtagcg ctgcttcaat attcaggtga cgtatttcca gacggaagcc ggcgcgcgat
     2821 cgtaaagttt gttcattgtc agttctgcca gccggttgtc aacggcagag ttaaaggctt
     2881 ccagctccgc aagggtaagt ttataacgcg tgtgggaatt cacttttccc gcgtctcctg
     2941 acgtattacg ggctgccact tacctggtgt cccgcaatac agccttagtg ttccgttatc
     3001 ggggatccgg acgcaccgca acaaccgggg aagtatttct ggtttagagc agtgtcaaga
     3061 gggatttctc agcccctcac tcaccggctc agtctcaggg caggagcatt aatacgctca
     3121 gttcatgtct tatacaggaa tgggcacagg gggagctggc gtgtcactgc tgattttacg
     3181 catcagcgtt catgtatctg cactctgata catgaacgat tcacagcgct ctgttaccgg
     3241 agagatttac tctcttagct tactctggca aatcctttac gttactctct gatgacttct
     3301 tatatttata ataccaatca gacagcctta tcccatcggt gatatcagtc tcctcctcgc
     3361 caggaacggg ctgactcact gtaactacct ccgaaaacaa ctctgcaatt atcagaacgc
     3421 ctgcttctct ccctgtcctc acgaaaacta tcccctcttt atcgcgcgtg cgtgcggaag
     3481 catcttttcg caacaaccac ccggtatacc ggtacggctc agccaccgca acctccccgt
     3541 ttatctcctg gcggtcacat tcccgattat tttttacgtt tctccccggt tgttatgccg
     3601 gtgaaggtgg cgcgctgttt tcatgaccac accggctggt ttaacaacat ccggaggaac
     3661 attctcatgg ctaccccagt ttcactgatg gatgaccaga tggtcgacat ggcatttatc
     3721 actcaactga ccggcctgac agataagtgg ttttacaggc tcatcaggga tggggccttt
     3781 ccggctccca tcaagctggg ccgcagctcc cgctggctga aaagtgaagt ggaagcctgg
     3841 ctacaggcgc gtattgcaca gccccgcccg taatttctga ccgttatccg ttcacccgca
     3901 gcagacgtaa tccccggcct gccggcggca ttctgctgcc tgttatatcc ccgtgaggaa
     3961 tatgaaaatg aaacaacagt atcagacccg ctacgaatgg ctccacgaaa gctaccagaa
     4021 atggctgacc ggcttcaccc ggcacgccgt atcctggggc gtgtgtcatc cgaatatcaa
     4081 ctacttccat aatctgacgc ccggttgggt gtcattcaat ggtgaaaaac cggagattgc
     4141 catcgtaccc cagagcctgc accggctgat ttatggccct gacaaacggg ccacgccgcc
     4201 cctggatgat gatctgatag tgaatttatg caccagtgaa catctgctgg ttcatcatcc
     4261 gatgctggaa ggcattctcc tgtctgaatg tgaacgtctg aggcagcgtt ctctggcaaa
     4321 taaactgatc agcctcttcc gtcagtttgg tggcacggag ttgcgcctca aactggtctg
     4381 gctttgctgg cttgatttaa tgaccggaaa cagccttgaa gactggaaag agaacctgaa
     4441 acggaaatca gaaaaagagc tggaggaatg gatcatcaac cgtcagaggc agagcgcagc
     4501 actgacggat ctgatggatc agtacgtgct cctggcatac cgcacaacgg ttgacgatag
     4561 ccgcaactga tgccggttgc ttccggaagg tgtgacaaaa tacggaaatg atgatgctcc
     4621 cggcccccat gctcacctgc agagcgtgtt cgtcgtcatc atactgagcc atgacgggcg
     4681 tcttaagggc atcctgtcat tgctgagcag gcaacaccac cagaggagcc acggacaggg
     4741 atgttcttta cagcccgata cacgccaggt tctcagcaga ctgaatgtgc aggtgcggtt
     4801 attcaccaga aatggcatgc tgcggtacat acccatcctg gtgacaatat gtacgtggcg
     4861 acagcgatcc cggcactgtg gaagagactc actcagcctg caggatattc tttccggcat
     4921 ccgggaatgt ccgcctgaat gcaaacagga tgatatttga atccgtaata ctgttctttc
     4981 atcataaata atttatgcag atacagttaa ccgttacagt agccggtttg ttttaccggg
     5041 aaataatatc cgtcagactg ataccggaac atatctgaaa cacgttatga tgttttgtta
     5101 tcagaaatgt tgtatattct tatccgtggc gacagtttaa tgccctctgg tatcacctga
     5161 taatgtatat cctgcgatga ttcgttatat ttacgggaca cgcctctttt cattgtgctc
     5221 cttttcagac ttcattttcc gtaagtgttc cccggcataa aggaggtacc tttctggcgt
     5281 ttataactct ctttatcggt gacgccggta atacagggaa taccacaccg acaaacgagg
     5341 tattaacaga cgatatttca gtgtccataa tttttctgtc cggttaaata acgtcagtct
     5401 gctgccctct gactgtaatc atcaaagccg gaatgcatac cgggttacaa cgtaaaaaaa
     5461 gtgatgctgg cccccggtgc agctggcggg cgaaacatgt ttacctttca ataccccctg
     5521 tctgcagtct ccactcttcc atcctcttaa gctacgtcgg ccacatgagt gcacttccat
     5581 gataacaggt attcctgtca gcataacgaa gccggttacc ttcccttcct cctgcatttc
     5641 ctgtcagaat gactatacag cgcgcagatt gcctttttat tgaccatctt aatataccgt
     5701 tataaacttc tttttatcat attattatca ccttattatg tttaattttt aagcagtcgg
     5761 agaaagagga tggttcagaa aattttgtcg gataaggtta tgaatgaaag aacaaatgcg
     5821 tattactctt attatcttgg agagcgtaat atctccgtac tgccccttaa tgtttatgat
     5881 cccccggaac gttttatcgc ttacataaaa aaaaacagag aaaatctgaa catcacactt
     5941 tcagattttg aacttgaaca aataatttct ggtatgcgtc tgaaggccct ggccttcctg
     6001 gtccccctgg aaaaaatatc ctggatcgcg ggcagtgaac gcgcatgctt attttcctgg
     6061 tatctgttga tgcagtttat ccagaataac agggcaaaaa taagcgcaga cttgcttcaa
     6121 aagaataaac tgtatcttaa agaagagtat ctggaaggga atgccttccc gtcggactca
     6181 tcgacacagt tcagacaaat actcagagtt ctcgatattc tttctgataa gaatttgcgg
     6241 gatgagtgga taatccagac caaagacaga tggatgcggg catttaaatc aaagagtcct
     6301 ttcagttacc tcctcccgga gaacgaacat gaatgcatat ggacatggaa ttatttgaag
     6361 ggaaaaaata ttgcactgga aaaactggcc agttttcctg gttctgctga tatttaccac
     6421 gcaattcacc tttcatttga catatgggtt acctgtccac tcacctcacc tgacgatata
     6481 aaaaatttca gaaacagttt caataaggcc aaggcgcagc gcaagtacaa aaaaatgcag
     6541 gaagataaag tcaatgtaca gttttttctt gacgcagaaa ccagggcgca gctcaaagaa
     6601 ttatcgaggg tcagaagact cagtaccggc gaaatgctgc atgacctgat tgttgaagag
     6661 tataaaaggt accgacattc cagataattt cagacatata ttatctccgt gagtcccccg
     6721 ccacctttcc ggtgtgc
//
LOCUS       DAJTRD010000160         6730 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTRD010000160 DAJTRD010000000
VERSION     DAJTRD010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6730
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.160"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..483
                     /locus_tag="OO315_003848"
     CDS             <1..483
                     /locus_tag="OO315_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5892579.1"
                     /translation="GGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVA
                     AYLADEIAKQGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPA
                     FTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT
                     "
     gene            853..1677
                     /gene="gadX"
                     /locus_tag="OO315_003849"
     CDS             853..1677
                     /gene="gadX"
                     /locus_tag="OO315_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5892580.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            2045..2773
                     /gene="gadW"
                     /locus_tag="OO315_003850"
     CDS             2045..2773
                     /gene="gadW"
                     /locus_tag="OO315_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5892581.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(3136..6249)
                     /gene="mdtF"
                     /locus_tag="OO315_003851"
                     /pseudo
     CDS             complement(3136..6249)
                     /gene="mdtF"
                     /locus_tag="OO315_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(6274..6531)
                     /locus_tag="OO315_003852"
                     /pseudo
     CDS             complement(6274..6531)
                     /locus_tag="OO315_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
BASE COUNT         1765 a         1589 c         1604 g         1772 t
ORIGIN      
        1 ggtggtcaaa ttggtacttt tgccatcaac ttctcccgcc cggcgggtca ggtaattgca
       61 cagtactatg aattcctgcg cctcggtcgt gaaggctata ccaaagtaca gaacgcctct
      121 taccaggttg ccgcttatct ggcggatgaa atcgccaaac aggggccgta tgagttcatc
      181 tgtacgggtc gcccggacga aggcatcccg gcggtttgct tcaaactgaa agatggtgaa
      241 gatccgggat acaccctgta cgacctctct gaacgtctgc gtctgcgcgg ctggcaggtt
      301 ccggccttca ctctcggcgg tgaagccacc gacatcgtgg tgatgcgcat tatgtgtcgt
      361 cgcggcttcg aaatggactt tgctgaactg ttgctggaag actacaaagc ctccctgaaa
      421 tatctcagcg atcacccgaa actgcagggt attgcccagc agaacagctt taaacacacc
      481 tgataacata acgttgtaaa aaccgaatgc ccagccttta aaaaaacagc tgggcattcg
      541 gttgcttatt aatcgcaata atatattggc tgtctattca tcgtgttgat aagatatatg
      601 ttatatgaat gtttattaaa tgtagcgatt acctttaatg tcacgaaaag ggattatttg
      661 cttgctatta atttcactgc gttcttaaac acgtttttgt attataaatc cagtcatcct
      721 gcccgtagaa catcacacat tatcatcctg ttctcccgct tcgtttaaat ttatttatca
      781 atcaatttga cttaagaggg cggcgtgcta cattaattaa cagtaatatg tttatgtaat
      841 attaagtcaa ctatgcaatc attacatggg aattgtctaa ttgcgtacgc aagacataaa
      901 tatattctca ccatggttaa tggtgaatat cgctatttta atggcggtga cctggttttt
      961 gcggatgcaa gccaaattcg agtagataag tgtgttgaaa attttgtatt agtatcaagg
     1021 gatacgcttt cattatttct gccgatgctc aaggaggagg cattaaatct tcatgcacat
     1081 aaaaaaattt cttcattact cgttcatcac tgtagcagag atattcctgt ttttcaggaa
     1141 gttgcgcaac tatcgcagaa taagaatctt cgctatgcag aaatgctacg taaaagagca
     1201 ttaatctttg cgttgttatc tgtttttctt gaggatgagc actttatacc gctgcttttg
     1261 aacgttttgc aaccgaacat gcgaacacga gtttgtacgg ttatcaataa taatatcgcc
     1321 catgagtgga cactagcccg aatcgccagc gagctgttga tgagtccaag cctgttaaag
     1381 aaaaaattgc gcgaagaaga gacatcatat tcacagttgc ttactgagtg tagaatgcaa
     1441 cgtgctttgc aacttattgt tatacatggc ttttcaatta agcgagtcgc agtgtcctgt
     1501 ggatatcaca gcgtgtcgta tttcatttac gtctttcgaa attattatgg gatgacgccc
     1561 acagagtatc aggagcgatc ggcgcaggga ttgccgaacc gtgactcggc ggcaagtatt
     1621 gttgcgcaag ggaattttta cggcactaac cgttctgcgg aaggaataag attatagagt
     1681 tttactcaga cataaaaaaa acccggcata ggggaccggg aagaggatag tctgccgtct
     1741 ccagactaat aaaccgttat aacactccct gttggcacgg gaaactttgt gctctcagta
     1801 agttaaatat aacttttaca ggaaataaga tcagccattt ttttataaac ataagctata
     1861 cgctgtgcga aaatatattc gtgctgcatt tacttattat caattaactg ttatgcaaaa
     1921 ctactttgtg gataaatttt ggtcctacca aatctggcag tttttgcgct aagaaacagt
     1981 ctgtcatcat ttcattagta tactgaaatt gaaataatcg cagtatgaaa tataagggat
     2041 aatcatgact catgtctgct cggtgatcct cattcgtcgt tcattcgata tttatcatga
     2101 acagcaaaaa atatcgctgc ataacgagag tatcctgctg ctggagaaaa atttggcaga
     2161 cgattttgcg ttttgttcac cggatacgcg acgactggat atcgatgagc tgacagtttg
     2221 ccattactta caaaatattc gtcagctacc acgcaattta gggttacata gcaaagaccg
     2281 tctgttaatt aaccagtcac cccccatgcc gctggtgacg gcgatttttg atagcttcaa
     2341 tgaatccggg gtaaattcac cgatactgag caatatgctc tacctttcct gtttatcgat
     2401 gttttctcat aagaaagaac tgatcccctt acttttcaat agcatcagca ctgtttcagg
     2461 aaaagttgaa cgccttatta gctttgatat cgccaaacgt tggtatctgc gcgatatcgc
     2521 ggaaagaatg tataccagcg agagtctcat caaaaaaaag ttgcaggatg aaaatacctg
     2581 tttcagtaaa atattactcg cttccaggat gtcgatggcc agacgattac tcgagttacg
     2641 tcaaattcct ctgcatacta ttgcggaaaa atgtggctat agcagtacgt cgtactttat
     2701 aaacacattt cgacaatatt atggtgtaac gccacatcag tttgcgcaac attcgccagg
     2761 taccttttcc tgacatattt tgcatttgaa tattggtcag gatctcacac ctgcttcatg
     2821 tgaaactctt ccctgatgat ttctgccggg ctaccggcta gttctctttc gcagtttgcc
     2881 aggcggtata ggtattttta ggaggaaaag actcggcatg tttgggatta ttaagctgac
     2941 aattcatacg attacgggaa tgtgggtcag tattgtgttg ttcaaattga tgacgaatgg
     3001 ctggagtggt ttctactttc agtgctgtgt actaagcctg gtattcctga cggttagctg
     3061 gttgttgagt ggtgaatggt tagcaggaaa gagtaaggct gaaccttcac gttcaacctt
     3121 actctcattt acacgttacg cttttttaaa gcgggcaaag agatgttcca ctacaacgaa
     3181 aaagaccgga acgaagtaaa ttgccagcac tgttgcggca aacatcccgc ccatcacgcc
     3241 ggtacctacc gcgttttgcg cgccagaacc ggcaccatga ctgataacca gcggcagcac
     3301 gccgagaata aaggccagag aggtcatcag gattgggcgt aaacgcatcc gcgccgcttc
     3361 gatgattgcc tctatcggcg ttttcccttc tttctgcatc atctcaacgg caaattcgac
     3421 aatcaggatg gcgttcttgg cggaaagccc gatggtggtc agcaaaccaa cctggaagta
     3481 gacgtcatta cttaagccgc gcagatcggt ggccagtaat gcgccaacga cgcctaacgg
     3541 aacaaccaac atcaccgaga acggaattga ccagctctca tagagtgcgg cgagggcgag
     3601 gaacaccacg accagtgaaa tggcatacag tgcaggagcc tggtttgagg ataacgcttc
     3661 ctgatacgac agtccggtcc atgagtagcc gacgcctgcc ggaagtttag cgaccaggtc
     3721 tgccataaat ttcatagcgt caccggtact tttcccggcc gccgcttcac ctaaaatctc
     3781 cattgacggg atgccgttgt agcgttccaa tcgcggtgaa ccataggtcc attcggtaga
     3841 cgagtaggca gaaagcggtg ccatcgtgcc agaggcgttg cgtacatacc attggttgat
     3901 gttatccggc aacatacgga acggcgtgcc tgcctggaca tacacttttt tcacccgccc
     3961 ctggttgagg aagtcgttca cgtagctgct gccgaaggcg gtggaaattg tctggttgat
     4021 atcagacagc gccacaccca ttgcctcagc tttcgcggcg ttgacgttca ctttgaacat
     4081 cggcgtatct tccaggccgt tcgggcgtac cccggtgacc tggtcaggcg attgcgctgc
     4141 cagtgataac agctcgtttc gtgcctaggt tagtttttcg tgccccaggt taccgttgtc
     4201 cagcagttcc atatcaaaac ctgacgcggt acccagttca gccaccgcgg gtaagttgaa
     4261 cgggaagacg acggctttat tgatactgct taacgcaatc attgcccgct gaatgatcgc
     4321 ggtaaccgag ttttcctcac cgacacgttc agaccacggc ttgagactga taaacgccag
     4381 gccgttgttt tgcccctggc cgctgaagcc aaagccgcca acggtaaaca ccgactgaac
     4441 attatctttc tcttttgtaa gataataatc cgtcacctgt tgcagtactt tcgtggtgtt
     4501 aaccatggtg gcaccggaag gtaactgcgc ggtggtcata aataccccct gatcctcttc
     4561 tggtaagaaa gaggtcggtg tacgcaggaa cagcaccgcc atcccggcgc aaatcagcag
     4621 gtagaccacc atgtagcgac cggtacaacg caacagcgag cgggtgctat cggtatagtg
     4681 ctgagttgat ttttcaaaca gcgtgttgaa gcgtgcgaac agggcgttag gtttgtgacc
     4741 gccttccggc gtggctttca gaatggtggc gcacagggca ggggtcaggc tcattgccac
     4801 aaatactgaa agccgcatgg aggagatcag cgtgatggag aactggcggt agatctcccc
     4861 ggttgcaccg ctcataaagg ccatcggcat aaacactgca gaaagaacaa cggcaatccc
     4921 gaccagcgca cgttggatct gccccatcga tttatgcgtc gcttccttcg gcggtagctt
     4981 atcttccgca atgacacgct cgacgttctc caccaccacg atggcgtcat ccaccagtaa
     5041 ccctatcgcc agcaccatcc cgaacatagt caacgtgttg atggtgaaac cgaccgccga
     5101 caagatcgca aacgtcccga gaataaccac cggtacggca attgtcggga tgatggtggc
     5161 acggaaattc tgcaaaaaca gataaatgac caggaagacg aggatgatag cctcaaccag
     5221 tgttttgaaa acttcctgaa tagaaatttc gataaacggc gtggtgtcgt aaggataaac
     5281 cgtcttcaga cttgcgggga aataggctga taagcggttc agctcctctt tgactgcccg
     5341 cgaggtatcc agggcgtttg ctccggcagc cagtttgatg gcgatcccgg caacaggttt
     5401 gccgttatag cgcgccacgg tggaataatc ttccgcccca agttcgacgc gggcgacatc
     5461 acgcagcagc acttgcgaac catcttgctg aactttcaac aggattttgc caaattcttc
     5521 cggcgtttgc agacgcgtct gcacaatgat cgaggcgttt agttgctggt ctgccgcctg
     5581 tggcatgcca cccagttgac caccagaaat ctggttgttt tgcaccttaa tctgggaaat
     5641 aacatcggaa ggtaccaggt tatatttatt gagtttttgc gggtccagcc agatacgcat
     5701 ggcgtactcg gaaccaaaga gctgtacgct accaacgccc gcggtacggc ttagcgggtc
     5761 tttgatatta gacgctacat agtccgcgat atcgtactgg ttgaggctgc cgttatcaga
     5821 aataaacgcc gctaccatca ggatattact gctcgactta tcgacgctaa tcccctgttg
     5881 ctgcaccgct tcaggtaatg aaggcatagc gagttgcagt ttattttgca cctgaacctg
     5941 tgcgatatca ggagatgccc cagtctcgaa ggtcagagtg atagaggcat tacccgccgc
     6001 atcactggtt gaagacatgt acatcaggcc atcaagccca ttcatatttt gctcaatcac
     6061 ctgagtgacc gagtcttcta ccgtttgcgc atcggcaccc ggataggtag cgctgacggt
     6121 aatggtcggt ggcgcaatct gcggatactg cgcaaccggt aagttcatga tcgccagacc
     6181 acctgcaagc atcataataa tggcaagtac ccaggcaaaa accgggcgat caataaaata
     6241 gttagccatg aaagtcccct taagcctgca acgttattgt ttcgattcgg tgctggcgtt
     6301 ttcctggctg gaggaaattg ctcgtgcttt gatacccgga cgaatgcgtt gcaaaccgga
     6361 aacgatcacc cgatcgccag cctgcaagcc agaggtgaca acccactggt cgccgatggc
     6421 tttgctggct tcaatttcgc gtagctgcac gacatcgtct ttatccagaa tgagcgccgt
     6481 tgctttaccc tgggcgttgt gggtgacgcc ttcctgcggc accagtaata taaagcgcgc
     6541 aaaattattc aagatatttt tgaaaatcat tcaagatagg ctatcgcgtg tgatagcaaa
     6601 aacattcaag ataaaaatgc ttgattatta agcaatcagg aatgggagta ttaagtttcg
     6661 tgtttccagt aaaaccagag agtattgaaa tagctatcac ctaactttat caggcgatag
     6721 ctggtagatg
//
LOCUS       DAJTRD010000161         6520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTRD010000161 DAJTRD010000000
VERSION     DAJTRD010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.161"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="OO315_003853"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="OO315_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5892582.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIAMIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="OO315_003854"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="OO315_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..>6520
                     /gene="narH"
                     /locus_tag="OO315_003855"
     CDS             5646..>6520
                     /gene="narH"
                     /locus_tag="OO315_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5892583.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1557 a         1712 c         1691 g         1560 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc atgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg gctcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct
//
LOCUS       DAJTRD010000162         6435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTRD010000162 DAJTRD010000000
VERSION     DAJTRD010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.162"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(150..511)
                     /locus_tag="OO315_003856"
                     /pseudo
     CDS             complement(150..511)
                     /locus_tag="OO315_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(850..1404)
                     /locus_tag="OO315_003857"
                     /pseudo
     CDS             complement(850..1404)
                     /locus_tag="OO315_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            1547..1669
                     /locus_tag="OO315_003858"
                     /pseudo
     CDS             1547..1669
                     /locus_tag="OO315_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1931..2497)
                     /gene="ipgB2"
                     /locus_tag="OO315_003859"
     CDS             complement(1931..2497)
                     /gene="ipgB2"
                     /locus_tag="OO315_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5892584.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2779..3093)
                     /locus_tag="OO315_003860"
     CDS             complement(2779..3093)
                     /locus_tag="OO315_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892585.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(3171..3281)
                     /locus_tag="OO315_003861"
                     /pseudo
     CDS             complement(3171..3281)
                     /locus_tag="OO315_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="OO315_003862"
     CDS             complement(3605..4282)
                     /gene="ospD1"
                     /locus_tag="OO315_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5892586.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4654..4791)
                     /locus_tag="OO315_003863"
                     /pseudo
     CDS             complement(4654..4791)
                     /locus_tag="OO315_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4873..5171)
                     /locus_tag="OO315_003864"
                     /pseudo
     CDS             complement(4873..5171)
                     /locus_tag="OO315_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5760..5919)
                     /locus_tag="OO315_003865"
                     /pseudo
     CDS             complement(5760..5919)
                     /locus_tag="OO315_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5923..6435)
                     /locus_tag="OO315_003866"
                     /pseudo
     CDS             complement(5923..6435)
                     /locus_tag="OO315_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         1723 a         1437 c         1315 g         1960 t
ORIGIN      
        1 ccggctactc attccctgct tgtgcaggat tttaatttcc ataactgtct caaaagtgac
       61 cataagctct cctgaatcag gagagcagat taccccctgg atctgatttc aggcgttggg
      121 tgtggatcac tattgcaccg ttcgtgacag tgttgttgcg ccttgctgcc aacgaagctc
      181 cactgcccat caagttcaca gataagggca acatcagcgt gagcgactgg cgcagatgtt
      241 atccgccggg gcgggagttt tttaatgtcc gtatgacggt attgatgccg attttcagcg
      301 tacggccagt atcgcgaact ccggcaccgt tgaaagccat ttcagtgatt tggtttttaa
      361 ctcccggttt acgggcctcg taacagtaag tgagctgaaa cacccggtga cagtcacggc
      421 aacggaatcg atcatgtcct tttgggttct gcccgtgacg gtaaacctga acggactggc
      481 aacgggggca atgaaagtta actgtgacca tgaaagaacc taaaagcggg tattataacc
      541 cctaattcaa ctaattggag gcattgtcat ttttaatcta tggatacact taaaaatcct
      601 ctcaccttac gttaaaaacc ttctcatgtt tagagttttt tattatgttt atcataatga
      661 tatgacgatc tgagttttta aaatcaagaa gaaaagataa gttttttaaa tgcacagtgc
      721 ctgtggataa ctttgcgtaa atccggagga cagatcactc tgaagcaaga caacagcatc
      781 tgaaaacaat atgctcacgt ttcaaccaca gggcgtaagt gatcctaccc acgtaatatg
      841 gacacaggcc taagcgaggt tctggttttc aaattgttcc ggactgaggc cgccacacca
      901 actgtgccgt cgccagcgat tgtaatcaca ttcgatataa ttaaacactg ttgccctcat
      961 tatttcccgg ctgataaagt gttctccgtg gagacattcc actttcagcg aatgaaagaa
     1021 gctttccacg caggcattat cgtagcagca gccctttgcg ctcatactgc cacgcagatt
     1081 atgccacttc agcagcgcct gataatctgc tgaacagtac tgacttcctc ggtccgagtg
     1141 aacgataacg ttccggggtc tcttacgccg ccacagcgcc atctgcaggg catcgcaggc
     1201 cagttgcgcc gtcaggcgtg gtgacataga ccagccaata acggcacgtg accacaggtc
     1261 aatgaccaca gccagataca gccagccttc atctgtacgt aagtacgtga tgtctcccgc
     1321 ccacttctgg ttcggaccac tggcgtaaaa atcctgctcc agcagatttt ctgacacagg
     1381 caggccatgt gcgcggtaac tgaccgtatt gctgggtaag atcaatttgc tcactggtat
     1441 ttactacgtg catcgaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt
     1501 tccgtaagca gccggagagt gaaactctct ccggctgact cgactcgcag gttccgacag
     1561 cggtgacgaa catgcagcgg tgttgttctc gctgatcggc acatgccgtc tgaacaatgt
     1621 ggagccagaa aaatggctgc gttacgttat tgaacatatc caggactgac cggcaaaccg
     1681 ggtacgcgat ctgttgcctt ggaaagttga tctgacttct cagtaaatat caatatgatt
     1741 ctgacgagcc gcttaccttt gagcagagaa aggggagagg gatcccacat ccatgagcta
     1801 ggaaaataca aaaactggct atacaacaat tcaatatatt aggacttact tgcgaggaga
     1861 aatatagcac cgctcaggca attcctgggt atttactttt cttagtttct ttactcggga
     1921 tgttctgtca tcagaaaggc gattctaaat ttgtaatata gtcactcata ttcccacgta
     1981 atttctttag atcaatatat tgcacatttt tctcaaaaaa ctgatcggaa ataagatttg
     2041 caacattgct tgttgtatca tttttaacgc ttctattcat cccttcacaa agttcgtcca
     2101 ttttcttttt gaaataactg cttcctgata caagatgatt tatggaactt tgtgcagcat
     2161 tgatgtctag agaaatgcga taatctttct caacctgttt aaatatctgt ttacggagat
     2221 tatcgccgat ttttcggctg tgttctgcac aaaacttatc aatacattgg tttaccaccc
     2281 gggatacgta gacttttttt tcatcatttt tccaatctgt aatattttca ccgatttgtt
     2341 ctgaaacaga gctcaaaata ctagtattta ttttggttga tgatattttt actatccttt
     2401 tccccattgt acagcggata atctcatcca cctttcctga aaaatatctt ttgtgagata
     2461 aactgattcc aaaattatta aaagatgttc caagcattat aatcaccttg tcatgtaaaa
     2521 tagtaacatt gtcattattt tagctgattc aaacaattct gtaaatgctt tgagttcttt
     2581 actcccttgg ctcaatgatg gaccgtctgg aatcccagag actaaactct ctgagaaaga
     2641 gagtatgaaa aaacttgggg cagtccctaa atgaagggac caacagcggt aaaataaccg
     2701 gcatcattca taatgatgcc ctgttagttg tagttatgaa gatttacgtc attaaaattg
     2761 ctgttcgtag tgtcagtcct atggtctggc atcagctgag gatcgtagcc gatacatcac
     2821 tggcttcacg acattcgtgt tgaggctatt catgaaaact ccacgctgaa aacgccgttt
     2881 tgtataaacg gtcacggtat gcccggagcc actgttgctg ataaattcga taaaaccttg
     2941 gcgtttcttg aagctatcgt taatgcggat aatgaaacaa cgattggtga gattcgctct
     3001 tttgccgacg ccctggacgc tgtccggttc aatcgcaaca aaatcaaccg gcagctgagc
     3061 aaacccaacc ttgcctctct ggcgctggag catgaagtga tttggctggg acgtagccgc
     3121 tgacagcttc tataaaagta tgatcctacc cacgtaatat gaacacaggc ttaagtgagg
     3181 ttctggtttt caaattgttc aggactgaga cctccacacc aactgtgccg tcgtcagcga
     3241 ttgtaatcgc acttgatata attaaacact gtatatttca tttgtggagt ctccagtctg
     3301 agagggcttt atgcattccg tattgggcac tatgatgcca gaaatctgca aggctccaca
     3361 ccttcctgta tttcccttcc tctatcacag tgcactttac ttaatagggt gggatgtgtt
     3421 cattacattt attgtgtttt ttatgattgt ttttgcgtcc agtgtcgttc gtcatgggaa
     3481 actggttcta tgggtagtat cgctcagtct agttaactgt ttgtttcttt ttagaaatta
     3541 agcagatcac acaattcggg gagagatcct tctaagtgca attctttgca gctatttagc
     3601 acacttaaaa caactcttgt attttgtcag aaataatatt aaacatgtct tgttcattat
     3661 tgttaagtct gtctgctatt tctgtaagtt ttatcccact atgatggcat atatttatat
     3721 atgccatcat agcctcgata ttccctttcc ctgcggccag aaataatcct ggtaacccct
     3781 cattatctct tacatccaat agtttaatcg tttcttttgg tgataaatca aaagttttaa
     3841 taaaatctcc ataagcttga atcgcagcgg aatgaccttc ttgaagagct atgaacaaag
     3901 cagattcccc agcattattt cgtgctgcaa gaacttctag tttttcctct ttgggtattt
     3961 cggctgccaa cagcgattca ccgtatttac gcgctgcatc ggcatttcca tgccaaatca
     4021 atgaaaacaa attatggcgg ttatactcct tattattctt tgtcggaatg gatatgttct
     4081 gtattttggc caattttctg aaagaaacac cactatatcc cccccctgat ttttcttcat
     4141 tgatggcgtg ggatatagcg gtatttgtca cgttaatgat attatttttc attcctttat
     4201 tttcattagc agtattgctg cctattatta agtgcatatt tttgttgttt gctggatgta
     4261 atccatagtt atttattgac atttttgttc atcctttttg ctaaaaaaaa acaataacct
     4321 atttttgtaa tgtaatagat attagtctat tgctaatcta aaaaaaccat tgccacattg
     4381 aatggctata ttcaccaata aattctttcg atttcgtata ttgacatatc gtagatatat
     4441 cttatcccta agattcaata atcgagtccg tttaccattc tgtgcaagcg ccttcactca
     4501 gaagtgaccg tcaaggcgat caccaaactt gataataaag cagctcattg ccatacgcca
     4561 gtccttaagg ggcgttgtcc atttctgtga tgctgcctag atttaactgc cagctccggc
     4621 ttatctacat ctaaatatgt gagcctaata ccgctacact ttgtccagac tatgtttttc
     4681 tccgggaagt gggctaaccg tctccataaa gtgacgaact tttttcaaca gtggccatat
     4741 tgaacggcac tgatgattac gggttattgt ctcatgaaga gccggccaca tcactgacag
     4801 cagtgacttc aggccttcag caccagacag cctgaaccag ttaatccatc gcccgacaaa
     4861 tttagtaatc tagggctggt ttttcttcag ctgttgcggt tcgaaagtgc cattacgatc
     4921 acgcagagta cggatttcca gaggttcatc tccagtagtg ataatcttcg ttaagcaacc
     4981 attgcgggaa ttgctgccag atctaagctg atttttctcg cacccaagat gatgagtcat
     5041 attagcattg agtacggttt cattttcagc atccaatcaa actgactgag atcttcagga
     5101 tttttgagac ttttggctag ttcattagct aaagcccgca acttattaga tatcaactgt
     5161 ttttcattca taaattcacc ttcatttgat atcaaattaa atagttcaac atcagagaat
     5221 tatacaaact tatgtacagg ctcctatgat cttcgaatcc tgaagattat agtgaaaata
     5281 agttttcacg ttgaaatccc acatagttag tatatgataa ataatcatgg atgacctgat
     5341 taacctcttg gggtagtgct tttatagaag aataaatatt ctactaattt aatacatcac
     5401 ctaaaatagt attgtttttt atggttggtt gaaggtcgtg gtgatgatgt tagtcctttt
     5461 gagaatattc cagtgccaga tcttacttat aataaagggg cttggcagtt tggataaact
     5521 attgttctta ttccactaat acaaactttt tgtaaagtat attaaatata cttcatttca
     5581 tactgaaatc cccggaagta ttcaatcaaa caattacacc ataaaaggaa gagggggccc
     5641 taattaagtt gtcaagcatg tttatgatac ctgcagggta taaattttct ggctgcaagc
     5701 agacacatat aaaacgtctt tattgatcct acccacataa tatggacaca gtcgtaagtg
     5761 aggctctggt tttcaaattg ttccggactg agaccaccac accaacggtg ccgtcgccag
     5821 cgattgtaat cgcactcgat ataactaaac actgttgccc gcactatttc ccgatggata
     5881 atagtgttct ccgtggagac attccatttt cagcgaatga attttcatct gttgctgtat
     5941 ccagtcatac agtgactgca tcagtggcgc ggctctggct tttcttgccg ccagacgctg
     6001 ttctgctgaa catccccgga cttctgcctc gatggcatac agttcaccga tacgctgcag
     6061 ggcttccgtg gtgatgtcgg ttggcactct tgcatgcaca tcgtggattt ttctccggac
     6121 atgagccata catgcggctt ccgttattct gccggattcg tacaacgccc ggtaaccacc
     6181 gtaagcatcg gcctgaagca ctccgctgta accggccaga tgattctgtg gatggatacc
     6241 tttccggtcc ggtgagtacg cgaaccagac cgccggaggc atttccgaac cggcgttacg
     6301 gtcatcacga acgtagaccc acagccgggc agtccttgtt ttaccgctgc ccggctcctg
     6361 gaccggaacg gggatatcat cggcatggac tttaccgggc atcagcacat actggcgcag
     6421 gatgtcatac agcgg
//
LOCUS       DAJTRD010000163         6308 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTRD010000163 DAJTRD010000000
VERSION     DAJTRD010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6308
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.163"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..312
                     /locus_tag="OO315_003867"
     CDS             <1..312
                     /locus_tag="OO315_003867"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5892587.1"
                     /translation="VAMIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKT
                     HDLLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNV
                     R"
     gene            complement(388..852)
                     /locus_tag="OO315_003868"
     CDS             complement(388..852)
                     /locus_tag="OO315_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5892588.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(849..1724)
                     /gene="nanK"
                     /locus_tag="OO315_003869"
     CDS             complement(849..1724)
                     /gene="nanK"
                     /locus_tag="OO315_003869"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5892589.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1721..2410)
                     /locus_tag="OO315_003870"
     CDS             complement(1721..2410)
                     /locus_tag="OO315_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5892590.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="OO315_003871"
     CDS             complement(2458..3948)
                     /gene="nanT"
                     /locus_tag="OO315_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5892591.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="OO315_003872"
     CDS             complement(4058..4951)
                     /gene="nanA"
                     /locus_tag="OO315_003872"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5892592.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="OO315_003873"
     CDS             complement(5073..5864)
                     /gene="nanR"
                     /locus_tag="OO315_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5892593.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1594 a         1702 c         1567 g         1445 t
ORIGIN      
        1 gttgccatga tttacattga acagcttgaa ttaatccata aaagtggtga tgtgttgtat
       61 ccagtaaaaa taaccagaaa atcatcagga aaaactgcct tccaccttgt tccattcggc
      121 ttaaataaga ctcacgacct tctagaggtg gaagatgctt cagaagcaat tcggctggtg
      181 attgatgaac gtcattccat tcgctgcagt acacttacag caactattac gaataaaaag
      241 ggcaagcgaa ttaagcggac aggtatatac agtatcaagg gtgttaacat aaaagaatac
      301 aacgtcaggt aatggcacag agcggtccgt cagttcaggt ttggctctgt gccaaagatg
      361 ggtcaggtca cacctgatct aataaattta agccattaaa tcagccttaa ccttcaccac
      421 caccttttta atctcacccg gctcgccaac aacgcatccc ggcttatgcg gctctcctgg
      481 cataaacacg gcgaacattc ccggtttcag gatgatggtt tgctcgtttt caatggcgct
      541 acaaagctgg taatcatctt catggtggaa ctcttcacat tggcgcgcag tgcctgccat
      601 gccaaacaga atccgttcct caccgtttaa taacagctgg atatcaatgt attgctcgtg
      661 caattccgct tttttctcga cgggcgattg agtgttaaac gtcatgacat tcataaaaat
      721 attgtcgccc tgtaattcgt aacgacccgg cgctttttct tgcggtctgg cagctaaggc
      781 cagcgttaac gcgtcctgta acgcaggatg taacccagca gacggtaatg actgtacttc
      841 acccatcatc ataatttttc tccctgggcc aacagcgcag ccccaagtaa acctgcatca
      901 tggcggtaat gcgccgccag taaatcaaca tgaaatgccg ctggctcctg cgccagatac
      961 atttccacca acgccagata cccttctgcc agaccaacgc tgccaccaac caccacgcac
     1021 tggcaatcag ttgtggcttt aatatcagcg atcagccttg caagcgtacg tgcggagcga
     1081 tgaatcagtt gctgcgcctg ctcatcgccc tgcccggcgc gtgtgaaaat agttctcgca
     1141 tccgcgccag ccagctcgcc ctgcgctgcc gccgcaatgc cgcgaccaga agcaattgct
     1201 tccacgcaac ctgtgcgtcc acagccgcag actgggccgt gtggatcggc aagcgtatgc
     1261 ccgatgtgcc ccgccagacc gccagggccg gtaagcagtt tgccgccgct cactacaccg
     1321 ccgccgacgc cagtggaaac ggtgataaag accatatcgg ttatatcgcc ttccagcgcc
     1381 tgatactctg cccatgctgc ggcctgcgcg tcgttaatgg caatggtcgg caaattggta
     1441 agttgttcca gcgttttgac taacggaaag tgtagcaatc caccgagatt atgcggattc
     1501 agcgccagca aactgccgtc gcgaataatt ccggttgaag cgatggcaac ccgctgcgca
     1561 tgagcttgca acggagagac taatgcggat aaggcatcac gcaaggcttc tggtgtctgg
     1621 ctggctggcg taggaagttc acgacgatcg cggatctgcc cgtcagcgcc aatcagcgcg
     1681 gcggcaagtt tagtaccgcc gatatcaatc gccagtatgg tcatagcacc gcctttttca
     1741 tcgctgtgtt gtaccactga caaatgtgtt caagacgcgt aatggcagaa ccgaccgtca
     1801 ccgcccacgc gccgtggcgc atcgcatctg ccgcctgagc aggcgtgttg taacgccctt
     1861 cggcaatcac ccgacatccg gcttcgctca acgttttcac cagcgccaga tccggctctt
     1921 ctggcgtttc aggcgtggta tagccagaaa gcgtagtgcc aataatttcg gctcccagct
     1981 tttggcatgc caggccgtct tccggcgttg agcagtcggt catcgccagt aaaccgtgat
     2041 gatggatacg tgccagcagc gtttcaacag gcaccggacg cgggcggtcg gtgccgtcaa
     2101 tggcgataat gtccgcgccc gcctgcgcca gcgcatcgac atcttcaata taggccgtga
     2161 tgcgtaccgg agaatcctcc agatcgcgtt tcacaattcc aataatcggc acgctcacca
     2221 ccgcacgcgt ggcttgcaga tttgccacac cttcgatgcg aatggcaacc gcgcccgcct
     2281 gctctgccgc taatgccatg gctgcgacga tttcgggttt atcgagcggg ctgtcaggaa
     2341 ccggctggca ggagacaatc aggccaccgt tagcagcgat tttttgatcc agttgtgcaa
     2401 gtaacgacat acatcttccc ttagcgaaag gcccggtaca tagaccgggc aacaggatta
     2461 acttttggtt ttgactaaat cgtttttggc gctgccaaat ggcacggcac cgctaaatgg
     2521 tttgccgtcg atagcgtcat gagtacgcaa tgcttccggg cgcagccaac gctgaacgcg
     2581 ggaaggcata tccagaccaa tcagcaggat caccacaaat gtcaggctga acgagagcga
     2641 tgccagcgca gtacccagat ccagacgttg agcgatcaac gcgccgatga ttggggccag
     2701 tgcgccaccc aatgcgccaa cgttgtaggt aaagcccagg cccgctgcac gctggtcggt
     2761 atcgaaataa ccgccaatca gtttcggtaa gatcccggcg atcccttgtc caagcatttg
     2821 ctggaagaac agtaacagac cgagcaccca gacgtttgcg ccgccaatcg caaataccgg
     2881 aataatcagc agctgcgagg ccagcaggct acaaacgtac gctttgcggg ttcccagcca
     2941 gtcaccgagg aagccaccta cgcagcatcc cacggccgcg ccaaagccac taaagaacag
     3001 cacattggcg acagtatgcg ggttataagc cagatcggtt ttcagatacg ttggcagcag
     3061 cgcctgaatc ggccatgagt agaggaaagc aaacaacacg accaccatca gcattacgcc
     3121 cgttggccag cgtttacccg tactttgcac cataaagctg ataaagattg cggcgcataa
     3181 cagcccgaga acagcgacga ttgcagcatt ttgcaggtta ccggcgaagc agaaccacag
     3241 cgcagtagcc gccgccagtg tcattacgat attggcaatg cgatgttcac cacggtagag
     3301 aatatccacc attgtgcgta ctggtgcttt acctgcgtgt ttctctttcc agtcttccgc
     3361 ttccgggatg tttttacgca gccagagagc aaagatgatt ggcaaaatgc cgataaagaa
     3421 cagcgcacgc caaccccaga ccggaaccac caggctatag acctgagcgg caacgacggc
     3481 ccccacagag aagcctgaaa tcaaaaaacc actggctttg ttacgcagat gttttggcca
     3541 gctttcaatg acataggtgg cgctggaacc gtattcaccc gccatcccca tgccgatgac
     3601 cagacgagcg ataaacatgg tgatgtagcc tggcgcaaag ccgcaggcca gcgtcccggc
     3661 cgagaagaga acgatgctgg tgaccattgc cagacgacgc ccgtagcggt cacccatagc
     3721 gccgagcatc aggccgccga accagcgaga gataaaggct gcggagatca gacttgccgc
     3781 ctgcaccgtc gtcagcccga attcaccctg tacttcggtg agtaccaggg cgattaaaac
     3841 gaaatcaaaa ccgtcaagca gatatcccaa ccaggcagcg gaaaatgcgc gccattgtgc
     3901 acggttgaga tggcgatacc acgggatatt ctgggttgta gtactcattg tgagtctccc
     3961 gcggtgggcg atgcccacac gctttggtat gaaaattgta gggtacagat gcgtttattt
     4021 cccctcaccc ggtaggggcg agcgagggga aaacaactca cccgcgctct tgcatcaact
     4081 gctgggccag cgccttcagt tctggcagat atttttcatc taccggtcca aacggtttgc
     4141 ggcacagcgg cacagaaacg acatccatat aatgcaggac agttttcagg ccgcggaata
     4201 cgcccgtttt gatcagtaaa tcaatgactt tgttgcactc agtttgcagt ttctgcgcgg
     4261 tctggatatc gccttctttc agcgccttca cgatcccctg atatcgccag cccatgatgt
     4321 tgtaagtact gccgatacca ccatcagcgc ctgccagcaa gccggaagcg aagatttcgt
     4381 cgtaaccgtt atagagcacc agatcaggat gttcacgacg gatctgttcc atctgataga
     4441 gatcgccaga agtctgtttc agcgcaccga cgccaggcaa cgtaaccagg gtgttgatct
     4501 gatccagggt cagttttacc ccactcaggg ctggaatgtt gtacaccacc atcggcaaac
     4561 catccgctga atcaataatt gcccgatagt gatcgcagtg ttcttcaaag ctgaaaggat
     4621 agtagaacgg cgtgacggcg gagacggcat cgaagccata acgtttagcc gatgccgcaa
     4681 gttgttggct ttcggcggtg ctgacgcaac cgacgtgggc gatgagttta atcttacctt
     4741 tcgcctcttc ggcgacgatt tccagtacct gttcacgctc ggaaaggctt tgtacaaagg
     4801 cttcgccggt cgaaccaccc acgtataaac cgtcgatgcc ctgctgaata ttgaactgaa
     4861 ccaggcggcg cagactcgct ttatccagtg cttgttgttg atcaaaagga gtcaggagtg
     4921 cagccattac gccacgtaaa ttcgttgcca taaatacctc tgaagtgatg cttgtctgat
     4981 aaacgatata cctttatacc tgttatacca gatcaattaa gcaacacccc atacagaaag
     5041 cttataatgc gatctgcttc actaaagtgg cattatttct ttttgttggt ggtctgaccg
     5101 aaagcgtgcc aggtagcaga gacgctgttg agatgcgatt gcaacgcacg atcggcttcg
     5161 tcaggatcat gacggcggat cgcatcaacg atcgcaatat gctgttgata actaacgttg
     5221 ttatgttcgt gcagtgcctg atcggtaacc gttgggcgtg cggcaataag ccagtcgagc
     5281 agggcaacgt ggatcgccat gaagattggg ttaccgggga tctccgccag cacgcggtgg
     5341 aaatcaacgt ctgaacgaat gaatgccgcg ttgttatcca gcgactgact gttgatttcc
     5401 agtgcttttg ccagcaaatc gatttgctca tcggtggcgt gttcagccgc atagcgcacc
     5461 aggctggatt caaagaacag acgtaattgt tcgaaatggg caatcccacc aggatgagaa
     5521 aggaaatctt tcgccatgcc ggaaagctcg ccgatgatag tgtccgcaga aggtcgagag
     5581 acacgagcgc gttcgccgtt gtttatttgc accagacctt tgcgttttaa cgctgccagc
     5641 gcttcacgca cagaaggacg cccgacgtta aagaacgcca tcagttcgcg ttcagacggt
     5701 aattgttcac cttcgccaaa ttcacgacgg cggatcatct gttccagctc ttcttccacc
     5761 atttcggaga gttttttacg cgccagcggg cggctacgca agttgcgacc aattgcaggt
     5821 gaagaatctt cggtttgcga atcaaatgcg ttcataaggc ccattctgta aggtcagtgt
     5881 gattaacatc atcagtgaca tcctatcaca ggattgaaag taggggaaaa tggcaggttt
     5941 ttctctttgt gcctcatcat taccataatt aacggaataa ttaactattg cgaaaaatta
     6001 atgtaacgca gataaacaca tcccgattga attattcata agactattca caagcattat
     6061 gaatattatg aatgtgttct tacaaaataa tcataatcgc atatttttta atgaaaaatc
     6121 acctcaccta caattaaaaa cacgacatcc gcaccataaa tagacttgca aaaaatataa
     6181 catcattgtt ttcaatctgc catttatggg attgaccgtt ttcttttgac acggagttca
     6241 acaatgggta atgcttccaa cttactgatt tagtgtatga tggtgatttt aaggtgcttg
     6301 cgtggctt
//
LOCUS       DAJTRD010000164         6095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTRD010000164 DAJTRD010000000
VERSION     DAJTRD010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.164"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(1..393)
                     /locus_tag="OO315_003874"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO315_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO315_003875"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO315_003875"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5892594.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO315_003876"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO315_003876"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5892595.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..6095
                     /locus_tag="OO315_003877"
                     /pseudo
     CDS             5797..6095
                     /locus_tag="OO315_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1867 a         1387 c         1047 g         1794 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg aggtgggctc accaccgctg
     6001 aacgtcagcg tctgaaagag ccggaacgtg aaaatcgtga actgcgccgc agtaacaata
     6061 tccttcgcct ggcttccgct tattttgcaa aggcg
//
LOCUS       DAJTRD010000165         6058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTRD010000165 DAJTRD010000000
VERSION     DAJTRD010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.165"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..143)
                     /locus_tag="OO315_003878"
     CDS             complement(<1..143)
                     /locus_tag="OO315_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR5892596.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQ"
     gene            379..651
                     /locus_tag="OO315_003879"
     CDS             379..651
                     /locus_tag="OO315_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5892597.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(617..1024)
                     /gene="nudG"
                     /locus_tag="OO315_003880"
     CDS             complement(617..1024)
                     /gene="nudG"
                     /locus_tag="OO315_003880"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5892598.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1111..1731
                     /locus_tag="OO315_003881"
     CDS             1111..1731
                     /locus_tag="OO315_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5892599.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="OO315_003882"
                     /pseudo
     CDS             complement(1740..3047)
                     /gene="ynjE"
                     /locus_tag="OO315_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(3114..3767)
                     /locus_tag="OO315_003883"
     CDS             complement(3114..3767)
                     /locus_tag="OO315_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5892600.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(3767..5238)
                     /locus_tag="OO315_003884"
                     /pseudo
     CDS             complement(3767..5238)
                     /locus_tag="OO315_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(5211..6058)
                     /locus_tag="OO315_003885"
                     /pseudo
     CDS             complement(5211..6058)
                     /locus_tag="OO315_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1427 a         1621 c         1599 g         1411 t
ORIGIN      
        1 atctggcgat attttggatt ttgttcaaga aaaggccaga gtgtggtcat tacttcacga
       61 acggcttgcg cgaactcggt ttgattcggg tcgcgctttt ggacatggtt gaggaatgac
      121 tccagagaat atgtctgatc catagatata aaaaccttat atattaatac gattgtgctt
      181 atgttgttta tgtcatgtgc ttttgcagtt ttcgactata ccatcaagaa tgtgacggta
      241 agcaagaaaa aaatgaccca ggaaagcaaa ttaataacga gagtaatctc ataaccaaat
      301 gcgataactc agacgctaaa gttttgccac ccggctaccg ttatcagtag cagagacaat
      361 aacgttaagg agttccctgt gagtcgagca ttgttcgccg tagtattagc atttccatta
      421 attgcactgg ctaacccgca ttatcgtccg gacgtggagg taaacgtacc gccagaagtg
      481 ttcagttcag gcgggcagtc agcgcagccc tgtactcagt gctgcgttta tcaggatcaa
      541 aattattctg aaggggcggt aattaaagcc gaaggaatac tgctgcaatg ccagcgtgat
      601 gacaaaaccc ttagcactaa tccgctgctc tggcggcgcg taaagccata aacgcctcta
      661 ataatggaat gtcagcaggg gccagcggat attgcagcgc ctcttcaggt gagcaccaga
      721 ccagcgcctg atgttcatgt gcctgtaacg tcccgtggaa gtcgggtacg tgccaggcat
      781 gaagatggat aatccggccc gaaacttctc gctgatggct ggcaacatac tcacccacag
      841 ttgcttcgat gtccaattct tcgtttaact cacgcaccag cgcctgctgc tggctttcat
      901 ccagctcgac tttacctccg gcaaactccc ataatcccgc ctgatcgctc tgggcggggc
      961 gttgcgcgag taaaattttg ccatcacgtt caatgatggc ggcaacaact tcaatcattt
     1021 tcataaatta cacacgggca gataagcgaa aaatgccata atatccgcac tgttgctcaa
     1081 tcagaaaggc tttatattta aggagtgcct gtgctagacc gccatcttca tccccggatt
     1141 aaaccgttgt tgcatcagtg cgtgcgggtt cttgataaac cgggcattac gccagatggt
     1201 ttaacattag tgggatttgc catcggcgtg ctggctctgc cgtttctggc gctgggctgg
     1261 tatctggcgg cgctagtcgt cattttgttg aacaggctgc ttgatggtct ggacggtgcg
     1321 ctggcgcggc gcagagggct taccgacgcg ggcggctttc ttgatatttc tctcgatttt
     1381 ctcttttacg cgctggtgcc gtttggcttt attcttgctg caccggagca aaatgcgctg
     1441 gcaggcggct ggttgttgtt tgcgtttatc ggcacgggca gcagttttct cgcctttgct
     1501 gcactggcag caaaacatca gattgataac cccggttatg cacataagtc gttttactat
     1561 ctggggggat taaccgaagg cacagaaacg atcttactgt ttgtgctggg atgtttattc
     1621 cctgcatggt ttgcgtggtt tgcgtggatc ttcggtgcgt tgtgctggat gacgaccttt
     1681 acgcgggtgt ggagtgggta tctgacgctg aagtcgctcc agcgtcagta attgcaatgt
     1741 tatttgctac tgcccgggcc gcgttcaccg gttgcaaccg gattttttgg atcgctgctc
     1801 cattcgtacc agccgccgtc atacacggaa acattcttcc aacccatggc gcgtgcgtac
     1861 ataaaggttt cggacgcgcg ccagccggtg ccgcagtaga atgaaacttg ctgatctggt
     1921 ttgatattcc atgctttcca catagcggta atatcatcgg cgctacgcat ggtgccatcc
     1981 gggttataga aatcttccat atgcgtcgag tcgctaccag cgtgtcccca acgtgctccg
     2041 gctatttcac cttttggttt aatatagctg taaccgctgg tcgtaccgat aaattctggc
     2101 cacgaacgaa tgctcaccag cgatgcatcc tggcgatgca gcagtccacg ggcctgttcc
     2161 atatcgagca tcaactgcgg ttgtgccggg atcttcacgc cgaaatccgg ttccgctttc
     2221 actttcggtg gcgttcctcg ctcaacaggc agtcccgcgt cggaccaggt ttgccagccg
     2281 ccatccagca gacgcacatc tttcacgcct gcgtagagca taatttgcgc cacacgcgct
     2341 ccagcgtata cgtcacgccc gtacagaatg accgtggtgt catggcgaat gccgtgtttt
     2401 gccagcatcg cttttagttg ttcatcagaa actttgttcc acagcggttc gctttccact
     2461 tcgttggtat cgatgtagtc agcgccagga atatggctga taaggtaaag cttaggagcg
     2521 ccctaggccg cttcaatgac tttccagtca ccgacaggtt tcgccgtaac ctctttacct
     2581 tgttgcaggt catgcagcca ttgcggataa accagctgct caaagtgcgg cagtttttgt
     2641 agacgggaag gttcgcttag cgcgtcactc agaatggaga tatgcgtaaa acctgctttt
     2701 tgcagtcgcg ttttgacggc gtcaacatct ttgccattac cgtacagcgc caccggggca
     2761 tcggctttca ggttatgttg cttgatccac gcgttgagct gttcggtgct cattttgtcc
     2821 agccagctag cagagaggtt taaggcggca ggttcatgac cagaagggcc atttaaggtt
     2881 tgtggccagc cgttataaaa cgcgctgggg cgagtatcta tcgctttgcc attttgttgc
     2941 tgaagttggt caagtgtaag aggcttcgcc agttcagcgg cccacgaaga agcgcaagcc
     3001 agccctaaag ccattgccag cgcggtcatt tgagaaacac gtttcatcga aaatcccgac
     3061 attgtagttt tgagtgcgcc attctgaact gacgcattga gaaaaacttt gcgttatcgc
     3121 agtttgttgt aattttctga ccactgcgcc atatccagaa cagaactatc agcaggaaca
     3181 tcctggagat cgtgcgttac ctgaacgacg gggatcgcca gggcgcgaac ttcgctgaac
     3241 acccactggc gaaaattatc gcgcagagcc acatcaagac ggctgaatgg ctcatccagg
     3301 agtaacgctt ttggctgggc gagaagggcg cgtagtagag caacgcgcgc tcgctgaccg
     3361 ccagacaaag tggcaggatc ctgatggaaa gctccgtcaa ggcctgaacg ctcaagtgca
     3421 tcattcacgg cattacgtcg ggcattcccc ttaagtgtcg ccggtagcgc cagcagtaaa
     3481 ttttgcccga cgctgaactg gtcaaataac agtgcatcct gaaaaagaat gccaatctga
     3541 cgctgtgcgg tgggtagcat gtcaatccgt tgctcattga gccatagctc acctgtacaa
     3601 gaaaactgtc cggccagtgc accaatcatc catgaaaaca gagtggattt tccacagcca
     3661 gacggcccca ttaacgtgac gatgtcacct ttatcaaccg taaagttaac gtttgtcagc
     3721 aagcggcttt ctggtaaacg tagcgaaaca tttttcacgc agagcattag cggattcctt
     3781 gtctgacata acctacccat tttgcgacta acgcggtcag ggcaaaaata ataagcggta
     3841 acaacagttg ccataacgcc tgggcggcga gaataccgtt gctgccgccg ctgcttaatg
     3901 ccaccgcctc agtggtgagc gtcggaaaac gccccgcgcc cagccacagc gtcggcatat
     3961 actgcgcaat actgactgaa aatcccaccg cgaaggcaat cagcacaggg cgcaacatga
     4021 gtgggcattt cacgtaaaag aagattttgg cccgcgacca gcccagtgtt tgcgcaatca
     4081 aaattaaccg tgaatcaatg cgctgccagg cgggttgcag gataaacagc atccacggca
     4141 ttacccacag cagatgcccc cagaccaccg ccgtccagct tccatccagg ttcagccata
     4201 gcgccagcgt gtactggccc gccaccagtg gcagagcggg caatagaatg ggcaaccata
     4261 gccacaactg gcgacgctgt gccccccatt ccagccacag cagtaacagg agcaaagcga
     4321 tgaatgttgc taccagcccc attgtcagac tgttgatgag tgcttcacta ttgatcgtcg
     4381 actgatccgc gaggatcgcc agcagaacca cacacagcac accggttaag ggtaaaaaac
     4441 tcgccagcgt agtgcctggt aataaaggtg tggcaggctt gcgaatgcca tctacgcggg
     4501 gaatagtgcg ccgccagctg cgccaaaacg taggcggcga gtaacagcat cagcagcaga
     4561 ctggcgagtg cgcctttcgt ttgttgatcc gcgtcgccct gagttaacca ctgccagcta
     4621 attactgcca gcgtcggtgg attacctggc ccaagaataa tcgccacatc cacgaccgaa
     4681 agcgaccagg caacaatcgc cagcatcgcc attgccagcg gctgtagccc agcgaatcca
     4741 gcacaatgac ctgctgcaac agccgttttt cgctcaatac tgccactaag atccacagca
     4801 gaaatgcgct ttctttcact gcaagggtga ggcccagacc gatccaaatc ggtccattgg
     4861 cggagtgaaa tacgggaaat agtcatatag cagccctccg tcagcaaaga gcagcagggc
     4921 gctggtggca aaagccacat ggggaatggc gagcagccac ggcagacggg cgcacatacg
     4981 ctgccatttc ggcccaggcc atagcgccac aatcaccagc agggcaatca acaatgcccc
     5041 gaccgccgcg atggttgtcg acaccagcgt tgccagtaat gcctgcggta actgtggatc
     5101 ggcaaataac gcctgccagc gtgtcaacga caacgcaggc gatatcagcg tgagagctgc
     5161 cgggatcagc ggtgcataaa tcaccgccac catcgcccac agcaaaaaga ttaatgcgta
     5221 ccgtaacggc gtaaccattc ttgttccagt gcattcaccc atcctgcgtg cggttcagcc
     5281 agtaccggcg gcagatcctg cggcattctt gattgcaata tttcgcgctg cccgtcaggc
     5341 agtttttgcg gatcgagaac agaaggatcg ccccagacaa cgggatctgc tttacgcagt
     5401 tgcgcatcgg gtgagagcag gaaattggca actaccttcg ccgcagcact ggcattcgcg
     5461 ttggctggaa tggtgacgaa atgcacgttg ccaatcatcc cctcgcgaaa gccaaaactg
     5521 taactgcttg ccggcaaatc gccgctggcg attttttgct gcgcatgtgc ggggttaaag
     5581 gtcagcgaca ggcgcaatgt gccggctttc agcagagcat ccatccgcgc gggcgaaggc
     5641 gggaaatcct ttccttcgcg ccacaaatac ggatgcagtg catcaagata ttgctacaag
     5701 ggcgcagtga cacgggcgaa agtcgcatcg tccggcgctt cttttaatgc ggcgggatct
     5761 ggcgtcagca taatcagcaa ctgttcaaga aacgccgtgc cggtaaagtc cggtgggcgc
     5821 ggataagtaa ccgtgccggg attggcttta gcaaactcca gtaaggcttg cggcgtttgt
     5881 ggtggctgtg gcgtaacatc gcggcgggcg ataaacgtca gttgtgcgcc gccccagggc
     5941 gattccgcac cttgtgtcgg cactgaaaaa tctccccgca ccggcagctg tgtgtcgaca
     6001 tagcgccagt tgggcagagt ctccgcccag cccgtttgta ataaattggc ctctttta
//
LOCUS       DAJTRD010000166         6011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTRD010000166 DAJTRD010000000
VERSION     DAJTRD010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.166"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            525..1703
                     /gene="ltrA"
                     /locus_tag="OO315_003886"
     CDS             525..1703
                     /gene="ltrA"
                     /locus_tag="OO315_003886"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5892601.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1786..1965
                     /locus_tag="OO315_003887"
                     /pseudo
     CDS             1786..1965
                     /locus_tag="OO315_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1995..2477)
                     /locus_tag="OO315_003888"
                     /pseudo
     CDS             complement(1995..2477)
                     /locus_tag="OO315_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2733..3713)
                     /locus_tag="OO315_003889"
     CDS             complement(2733..3713)
                     /locus_tag="OO315_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5892602.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3713..4912)
                     /locus_tag="OO315_003890"
     CDS             complement(3713..4912)
                     /locus_tag="OO315_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5892603.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5234..5572)
                     /locus_tag="OO315_003891"
                     /pseudo
     CDS             complement(5234..5572)
                     /locus_tag="OO315_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5639..>6011)
                     /gene="istB"
                     /locus_tag="OO315_003892"
     CDS             complement(5639..>6011)
                     /gene="istB"
                     /locus_tag="OO315_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5892604.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT         1639 a         1378 c         1423 g         1571 t
ORIGIN      
        1 gcagcataag ttatccatgc tctgaggtgg gtagaggtgg gccctccggt cagggtttac
       61 aggagcacag accaaacgac ccggtgctgc ttcattctaa ggaatgaggt ggtgagcgac
      121 tcgggaaaag gcagagatct actgtcacgt gggttcggga agctgaacga aagtaaactg
      181 tccgatgaag catcgaaatc ttagatgatg ccaaaatggg gccgtacagc cgtcccctga
      241 taagcgcaga agacacctgc ttactgactg cgcggcatcc ggtgtgaagg cagcaggatc
      301 ccgggccaga ccatctcagg gaacgtgaga acctgtttcc ggcacgtatc gggaaacagg
      361 ggcggaccca ttcgtagtag tgatgaaaga tctgtaatgg gtctggagcg aagggatggg
      421 gatcacattc attgcagaag acacaaccca aaaggggatg acgtaactga tgcaacgaaa
      481 atcatttgaa ataccaaaag cactggtctg ggcatcatat ctggatgtgc gtcgcaacaa
      541 gggagccccc cggatgcgac gggcagactt tgaaaatgtt cgaccagcaa cgtgatggca
      601 atctatacaa gatctggaac cggctatgtt cgggaacatg gtttccgcca ccagtgctgg
      661 aaaaacggat cccgaagtcg aatggcaagg aacgcattct ggggatccca acagtatccg
      721 atcgaattgc tcagggagcg ataaaacttt tcatggaaga aaagcttgac ccgattttcc
      781 atgctgattc atatggttac cgaccaggca aatcagctca tgacgctctg aaacaatgtg
      841 ccatccggtg ctggcgttat agctggatac tggaagtgga catcagtgcg tttttcgacc
      901 atgtgagaca tgatctggtg ctcaaagctc tggaacacca cgggatgcct aaatgggtca
      961 tcctgtactg tcgacgctgg atggaagcgc caatgcagag ttgtgaaaat ggagaattaa
     1021 taacccgaac acgaggcact ccgcagggcg gggtcattag tccgctactt gctaacttgt
     1081 tccttcacta tgcatttgat ttgtggatgg aaagagaata tcggggggta ccgtttgaga
     1141 ggtacgctga cgatattgta gtacattgtt cacgaatgag tgatgcaaca aggctaaaga
     1201 acaggttatc ggagcgcttc tcggaagtcg ggctggtcct gaatgcaggg aaaacgaata
     1261 tcgcctacat tgacacgttt aaaaggagaa acgtcgcaac gagtttcacc tttctcggat
     1321 atgacttcaa agtgcgtacg ctgaagaatt tcaacggcga acggtaccga aaatgcatgc
     1381 cgggtgcgtc aaatgcagca atgcgcaaaa taacagaaac aatcaagaag tggcgtatac
     1441 atcgctcaac agctgagagt ttgctggatt ttgcgaggcg ctacaatgcg atagtgagag
     1501 gctggatcga gtactacgga aagttctggt ccagaaattt caactatcga ctgtggagtg
     1561 caatgcagtc acgtctgctc aagtggatgc agtctaaata cagactttcg aaccggaagg
     1621 ctcagcgaaa gctgacgctg gtaaggaagg agtatccgaa gctatttgtt cactggtatt
     1681 tactacgtgc atcaaatgag tgatcaagag ccgtatgacg cgagagtgtc acgtacggtt
     1741 ccgtgagcag ccggggagtg aaattctctc cggctgactc gactcgcggg ttccgacagc
     1801 gatgacgaac atgcggcggt gttgtactcg ctgatcggca catgccgtct gaacaatgtg
     1861 gaaccagaaa aatggctgcg ttacgtcatt gagcatatcc aggactggcc ggcaaaccgg
     1921 gtacgtgatc tgttgccctg gaaagttgat ctgagctctc agtaaatatc aatacggttc
     1981 tgacgagccg cttacttttg gcgtaattct gctgcgcctt tactgaacac aatgccgtat
     2041 tcagcaagaa gtccacgaat gtgatttatc agttgtgtcc tttccattga aaccaaccga
     2101 cgcatattca aaaaagtctg gacgtcctgt tgctccagag tttttattgg tacgggtcgt
     2161 atagttccat gttggcaggc ctcagctatt gcctgtgcgt cgttataatc atttttctgg
     2221 caccgctgat aagctttaac atgttgtgcc ggaagcaata ttacatcatg gtcgagtttc
     2281 ctgatttccc gggcccagta atgagatgcc ccgccagcct ccatcgcaac agtcatttta
     2341 ggctgttgca ccagccagtt aagcagttga ttgcgagaaa gtttggtctt tcctgcgggt
     2401 ttgccatgag cattgatact gaaaagataa aagttcgttt tagccagatc aatgccggtg
     2461 attttctgtt cagacatggt atcccgctta cttttgttgt taaagtttat ccatcatggc
     2521 gcaattgaag gctgccgtga aagaggggga agactatctc atcggttact gttaaacggc
     2581 taagagaaac tatctttttc aagcaaagaa gatacggtga aacaggcgat ttcaccgtga
     2641 aatactgttc aaaaaaaacg ctaataaaag caaaatttct tttaaaacag ccaattaaaa
     2701 actggcgagt tgctggcgaa aaacttgaat tttcacagcg aattcagatt atcttttagt
     2761 acaagagcaa tcatacgatc taacttatcc tgaacttcca acggtaatct gccgaattca
     2821 tatgagaacg tacgtccctt tactcgtttt cgagcaaatt tatctttact atcaaatttc
     2881 cataacaaag ttacaaccgc tttatccttt actcctgaat caagcaatga agacatttcc
     2941 ttggttataa gccgcataat actgttttta acctcgtctt cagaaagatt gtcatcagca
     3001 gtaataagct caatactcgg cgagatattc ttcacaactt catcaataga tatatcaccc
     3061 agcctcagtc gttcacttaa ttcagccagt tgcctgtaat cggcgtatgt cagctcagac
     3121 tgaacaggaa aaagagacac aaaatcttta ggtacggacg ctgcttggag ggcacgagtc
     3181 accttagctg ccgacaatcc ctctctttcc gcaatttgct tctggttcat accggcctct
     3241 ttgagacgta ctaaacgcaa cccaatttca cgaatattat gttcaagtga cgtctgcaaa
     3301 tctttcgcca gatgctgagc ttcagaaaca gtaagctcat catccgttac aagtacacgt
     3361 aatccaacct tgcatagcaa agctgctgcc cgacgacggg aaccatccaa aatctcaata
     3421 agatcgccag tccgaatccc aatacatgga tagaattgtt gtaaacgtat agttctggtg
     3481 atgtctttta gtgagtccgg cgtcagggct gtctgttcgc ggccattcac ctcttcaacc
     3541 acaaaagttt gagtatccac tttgtcatga tcaaccttga tctcagtaaa ttttgccttt
     3601 cggccagtat tgagggtaaa tacatgaact ggagccgata tttcttcagt attattcagg
     3661 atatttgtat tcagcgtacg gccaatagtc ggtcggtgct ttctgttttc catcagtagt
     3721 tggcccttat aaattcaata cgatcaaaaa ctgctttagc gaaatcttct gctgccatac
     3781 gtgcattttt caaagcttca ccactgccaa cataagtaac aggattcgca gatatcacgg
     3841 tatcaaacga ctcgccgctt ctctcaaaac catcaagacg tggcattgaa acatcaagca
     3901 tgtcaccacc gaaaatctct tttgtcaggc tatggcagta tttatgatct gacttattcg
     3961 ccagtttcga catgaagcca atatttgcct gcaaacggca tgaacatcca tcctgttcaa
     4021 taatctgtac aagttcaggt aaacgagcca aatatttaag cgtggaatgg aaatcaactt
     4081 gtgctggcgg caccggtgta aacatgatat ctgcggcagc aatggcattt ttcaaaaatg
     4141 cgtccagatg cgggcctgta tcaataagga tgaagtcgaa atcatgcttc agcttatcaa
     4201 taatattctc acgcaatatc gcgtgtttgt tttgtcctaa tagatgctcc tcgcacagag
     4261 tatcccagtt tgatgcaata aaggcgtcgt caatagaagc cggcataaca taaacgccag
     4321 ggataactga cggaacgata aaatcttcca gtaattcctc acgactcacg ttctgcaaca
     4381 tagcctgcgg tgcggttgta tcaaccagcc ccaccgcatg caagtagttc agaaacatag
     4441 tggctgatga ctggggatct aaatcaagga gcagaatacg taaatcttct gacagcaggt
     4501 gaggatgagc acgcagggcg tgagctacgg aaacactact cacggtttta gacacaccgc
     4561 cttttagact accgatgaat atggaatatg cctcactata acgatcgcga tatttaggaa
     4621 tgccacgatg tgcatagata tcgataatgt tctggatggt taatgcatat tttgtcgcgg
     4681 ttcctgctgg gcgtttttca aactgatagc cctgagcctc catctcaccg actgcaccat
     4741 ccacgcttgc gcgcgttaat tttggaagct tagctacagc agactttgaa tagacttgat
     4801 aaaaaacatc agcctgaaac tcctgctttt gctcctgaat ctgttttgtt agggcgagca
     4861 acattttatc tgcacgctgc gcaaccttac ttaactgctc aaaactagtc attccccctc
     4921 cgtgctatgt aggattttaa cttattacaa aaaaaactgc attagcaggg taatgcatgc
     4981 aagtacatat tgtacatgct tatattaaaa atgcgcgcat tgctgattta ttaatcatcg
     5041 gaatgaatat tactgtaaga tagtatgcct ttccgggaga aagacgctga tttcccccga
     5101 ataatccagt tcaccaccac actccggaca acagctttct tcaggctcca gatggtgtat
     5161 ttcgcgggaa acatgttccg gtaacggacg gcgatgacgt gactgtcgca acaggcgggg
     5221 gtagtgcttc caatcagtag aacatgtgct tttcaatgaa ggagccgatg actttttcgt
     5281 ggatttccac ggaacgcgag aagcagattg ttttacgtgc cagccgcttg atgcgggtgc
     5341 ggagggtccg gttgttacgt gcaatacgtt gcgtgaagat tttgccggtc aggtgtttct
     5401 ccttcggcac ttctctggcg tagctgcccc agtcatcgct ggtcaccatg ccgatacaga
     5461 acggagtcaa cagggctaac agttcccgac aggtctcatc attccgtggc ccgaaagtgt
     5521 aagccaacac accgccagtt ttagtgttgt acgcatacca gagccagtgt tgtgtcaacg
     5581 acggatgaaa agtgatccac ttatatctcc accaacggcc caatattgat ccaccgtttt
     5641 actcaggatt agcttctgct ataaccccgg cctttcgttt ctgtctgagt cgatagcttt
     5701 ctcctttgat ttgaacgaca tgtgagtggt gtaagatacg gcccagcatc gctgaggtca
     5761 gtgctgcatc accggcgaac gtttgatccc actgcccgaa cggtagattg gatgtcagga
     5821 tcattgcgct cttttcgtaa cgtttagcga tgacctggaa gaacagtttt gcttcttcct
     5881 gactgaacgg cagatagcct atttcatcaa tgatgagcag gcggggggcc attactccac
     5941 gctgaagcgt cgttttataa cggccctgac gttgtgccgt agataactga agtaacagat
     6001 ctgctgctgt t
//
LOCUS       DAJTRD010000167         5996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTRD010000167 DAJTRD010000000
VERSION     DAJTRD010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.167"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..116
                     /locus_tag="OO315_003893"
     CDS             <1..116
                     /locus_tag="OO315_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5892605.1"
                     /translation="IAFEAGYSSPSLFIKTFKKLTSFTPKRYRKKLTEFNQ"
     gene            221..1573
                     /gene="celF"
                     /locus_tag="OO315_003894"
                     /gene_synonym="chbF"
                     /pseudo
     CDS             221..1573
                     /gene="celF"
                     /locus_tag="OO315_003894"
                     /gene_synonym="chbF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
     gene            1586..2344
                     /gene="chbG"
                     /locus_tag="OO315_003895"
     CDS             1586..2344
                     /gene="chbG"
                     /locus_tag="OO315_003895"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5892606.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2391..4652)
                     /gene="katE"
                     /locus_tag="OO315_003896"
     CDS             complement(2391..4652)
                     /gene="katE"
                     /locus_tag="OO315_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5892607.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4835..5098
                     /gene="cedA"
                     /locus_tag="OO315_003897"
     CDS             4835..5098
                     /gene="cedA"
                     /locus_tag="OO315_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5892608.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5383..5996
                     /locus_tag="OO315_003898"
                     /pseudo
     CDS             5383..5996
                     /locus_tag="OO315_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1529 a         1281 c         1525 g         1661 t
ORIGIN      
        1 atattgcgtt tgaggccggt tatagtagcc cgagtttgtt tattaaaacg tttaagaaat
       61 taacgtcctt tacgcctaag agatatcgta agaaattgac tgaatttaat cagtaagtcg
      121 ttatacctga caattcacat atctgtcctg ttgctggatt atttatgtcc gggggggcag
      181 atatgccaga tatcagtatt ctgtactgaa gggagaaatt atgagccaga aattaaaagt
      241 cgtcactatt ggtggcggga gcagctatac cccggagtta ctggaaggat ttattaagcg
      301 ttatcacgaa ttgccggtca gcgaattatg gctggtggat gtcgagggtg gcaaagctaa
      361 actggatatt attttcgatc tctgccaacg gatgattgat aacgctggcg tcccgatgaa
      421 gctttataaa acgctggatt gccgcgaagc attgaaagat gctgatttcg ttactaccca
      481 actgcgcgtt ggccaattac cggcgcgtga actggatgaa cgtattccat taagtcatgg
      541 ttatcttggt caggaaacca acggcgcggg cggtttgttt aaaggtctgc gtaccattcc
      601 ggtgattttt gacatcgtaa aagatgtcga agaactttgt ccgaatgcat gggtgattaa
      661 cttcactaac ccggcgggaa tggttactga agccgtttat cgtcataccg gatttaaacg
      721 ctttatcggc gtgtgtaata ttccgatcgg catgaagatg tttattcacg atgttctgat
      781 gctgaaagac agcgatgatt tatctatcga tttgtttggc ctcaaccata tggtgttcat
      841 taaggatgtg ctggtaaatg gcaagtcgcg ctttgccgaa ttgcttgatg gtgtggcatc
      901 cgggcagtta aaagtatctg gcgttaaaaa tattttcgat ctgccattta gcgaaggctt
      961 aattcgttct ctgaatctgt tgccgtgttc ttatttgctt tattacttca agcaaaaaga
     1021 gatgctggct attgaaatgg gcgaatacta caaaggcggc gcatgagcgc aggtagtaca
     1081 gaaagtcgag aaacaacttt ttgagctgta taaaaacccg gagttgaaag ttaagccgaa
     1141 agaactggaa cagcgcggtg gggcttatta ctctgatgca gcgtgcgaag tgatcaacgc
     1201 tatctacaac gacaagcaag ctgaacatta cgttaatatc ccgcatcatg ggcatattga
     1261 taatattccg gcagactggg cggtagaaat gacctgtaag ctggggcgcg atggcgcgac
     1321 gccacatccg cgcattacgc atttcgatga taaagtgatg gggctgattc acaccattaa
     1381 aggcttcgag attgctgcca gcaacgccgc acttagcgga gaatttaacg atgtgttact
     1441 ggcgctaaac cttagtccgt tggtgcattc cgatcgcgat gctgagctgc tggcacgcga
     1501 gatgattctg gcgcacgaga aatggctgcc aaattttgcc gactgcatcg cagagcttaa
     1561 aaaagcacat taaccgaggc tgattatgga acgcttactg attgttaatg ccgatgattt
     1621 tggcttaagc aaaggtcaga actacggcat tatcgaggcc tgtcgcaatg ggattgtcac
     1681 atcgacgacg gcgctggtga atgggcaggc tattgaccat gcggtgcagt tgagtcgtga
     1741 tgaaccgagt ctggccatag ggatgcactt tgtccttact atgggcaagc cactgacagc
     1801 tatgccgggg ttaacccgcg atggtgtgct gggaaaatgg atctggcagt tggcagaaga
     1861 agatgcttta ccgctggaag aaattactca ggagcttgcc agtcagtatt tgcgtttcat
     1921 tgagctattt ggacgcaaac ctacgcatct tgatagccat catcatgtgc atatgttccc
     1981 gcagattttc ccgattgttg ccaggtttgc ggctgaagag gggattgcgt tgcgcatcga
     2041 ccgtcagccg ctatctaacg ctggtgattt accggctaat ctgcgcagtt cgcagggatt
     2101 cagtagcgcg ttttatggtg aagagattag cgaggcactg ttcctgcaag tgctggatga
     2161 cgcaagccat cggggtgatc cttcgctgga ggtgatgtgt catccggcgt ttatcgataa
     2221 tactatccgt cagagtgctt actgtttccc acggttaacg gagctggaag tgctgacatc
     2281 agcgtcgttg aaatatgcaa ttgctgagcg tggttatcgg ttgggtagtt atcttgatgt
     2341 gtaagtaagg tggtaaggtc actccccagg ggacgtgacc ttatccatat ttacgccgga
     2401 attttgtcaa tcttaggaat gcgtgaccac acgcggtgtt ctgccatcag cgttaacagt
     2461 tcatccataa aactaccgtc ggtgctgtca gcttccacaa tcccttcttc accctggtca
     2521 gctaccttga ttgttgcttt aaacttgcgc gcgtctcccg ccagcgcaat cggtttaagg
     2581 tgtttgtagg cttccatcag atagtagttg gcatcgccgt tgtcagcgat atccgcgata
     2641 ttgccgcaag ggacaatgac cgcatcgacc gtcagcgaag gtgcaccggc aaaggtagcg
     2701 gctataggca acaccgtacc gtcatccgca gtcacttcac ccattcggga gtagagcagt
     2761 ttggcatgaa cgcctttggc cttcatggcc ttgagaatgg ccagaaggtc tgccgatctc
     2821 acttcatcat taagtaaaat cgctaccacg cgacctttca catcaccgtc aggaatggcg
     2881 tacaaactta aggatggatc ctttttcaga ccgttgacgt ccggaggtgg ggtgatattc
     2941 agctggtcgt cagtcagttc gataccgaga tttttcgcca ccgcctgggc cagagtgaga
     3001 tcaatatgcg ccagctggtc aacaacgcgc tcacgaatat acggacgaac gactttgctt
     3061 aactcaaaac tgaaaccatc gacaatatgg cgctgctcga atggcgtctg acttagccag
     3121 aacagacgcg gatgggaata atattcgcca aacgatgggc tgcgctcgcg aactttattg
     3181 ccttccacgc gctcttggta tgattcaaaa ccgccgcgtt tcggccccgg tggtgtttcg
     3241 cgcggccagt tatcgttgat cgagttcggt tcataattcg ccgggttagt gtcgatcccc
     3301 atacgatgca tgccgtcacg ctggaaatta tggtaagggc aggtcgggcg gttaatcgga
     3361 atctcatgga aattcggccc accaagacga ctgatttgtg tatcggtata ggagaacaaa
     3421 cgtccctgca acaggggatc gttggtgaag tccagaccag gaacaatatg acctggatgg
     3481 aatgctgctt gttcgttttc ggcaaagaag ttatccggat tgcgattgag caccattttg
     3541 ccgacacgct gaacgggcac cagttcttcc gggatcagtt tggttggatc gagaagatcg
     3601 aagtcgaact tgaattcatc ttcttcagga atcaactgga agcccagttc gtattccgga
     3661 aaatcgcctg cttcaatggc ttcccacaac tcgcggcggt ggaagtccgg gtcacgtccg
     3721 gtgagttttt gtgcttcatc ccaaacgagt gaggctttac ctgccagtgg tttccagtgg
     3781 aaacgtacaa acgttgcctt cccttcggca ttaatcaggc ggaaggtgtg aataccgaag
     3841 ccttccatgg tgcggtaact gcgggggatg ccgcgatccg acatcgccca catcacgttg
     3901 tgcagagttt caggttgcag agaaacataa tcccagaaag tatcgtgggc actttgccct
     3961 tgtggaattg cccagtgcgg ttctggtttt accgcatgaa caaaatcggg gaatttatgc
     4021 gcatcctgga taaagaagat tggcgtgtta ttgccaacga ggtcaaaaat accctcttca
     4081 gtatagaact tggtggcaaa accacggata tcacgcacgg tatcggcaga gccagcgcca
     4141 ccctgaacgg tagagaacct aacaaatact ggggtgattt tgttcggatc tgagaggaaa
     4201 tccgctttgg tgatatcgct taagctttta tatggctgga aataaccgtg agctgctgag
     4261 ccgcgtgcat gaacaatacg ttccgggatg cgctcatggt caaagtgggt gattttctcg
     4321 cgcagaataa aatcttccag cagcgttgga ccacggttac cggcacgcag tgagttttga
     4381 tcatcggcga tgcgcacgcc ctgattagtg gtcagcgcat aattttcact gcctttgcgt
     4441 acgtcttcca gagaattaag tttttcgtta cgtgtatcag gggctttcag gctcccaggg
     4501 gcggtaggtt gtgcacctgg cggtgttggt tcagccgctg gacgatgaga tccgtcctca
     4561 ggtgccagtg agtccatccc cggtttcgct tcgctggaat cgtgtagtgg tgactggtgc
     4621 tgatgtgggt tcttttcgtt atgttgcgac attgaactcg tctccttaat ttattactga
     4681 aagggccgct gttttggtca aaaaactctc taactataga ccaccagcca gatcccgctt
     4741 cggagactat tctggaaacg gtgttatgaa atacacgcaa agcgggacgt acaggggcta
     4801 aatcggctaa actacagttt ttctaatttt taatatgcgt ttagtgaagc cagtaatgaa
     4861 gaaaccgctc cgccagcaaa accgccagat tattagctat gtcccacgca cggaacccgc
     4921 gccgccagaa catgcgataa agatggattc gtttcgtgat gtctggatgc tgcgtggcaa
     4981 atatgttgcg tttgtactga tgggagagtc atttctgcgc tcaccggcgt ttactgtgcc
     5041 tgaatctgcc caacgttggg caaatcagat ccgccaggaa ggggaagtga ctgagtaagg
     5101 attacatacc tcccggtttt gccgggagtt acaggacctg aaattctgca tttcgttgct
     5161 acattcattc aaaccacctg taacaaaaag ctgcctttga cgagctattg ctataaataa
     5221 gagatctttt ttctttaatt atttatttta aaatggttaa gttttgctaa tcatgatgag
     5281 ttatttttga tagttcatcc tgtggcgtcg ctcttaaact tatattgtaa atgacttaga
     5341 tttaaataag ataataattt tgttaacaat atgaggcggg agatgttttc tacaaactta
     5401 atacagagta attatggtga tttaaatatt aaaagcctgg ctttcgactc ttttaaagaa
     5461 aggcttgagt ctaccatggc ggcactcact ttctttatat caacgggtca atgtgactgc
     5521 gatgaaatag cagagtcgaa cttcaatcgt atgattgcct atatgtctaa cattaactat
     5581 gatgcctcaa agctaggagc gccagcactt agttttgata cgtatctgca ggacaatgtt
     5641 aaatatagag tgataattaa taatctttat ggcagtgagt ttcggataag agatattaat
     5701 gagaattttg ttgggatgga ttttacctgt gaattccgac ccgaaaaaat gacgaatttg
     5761 atcaatataa aaaataggtt ggttattcat tatttaagga ctatatacta tgaaaaaaat
     5821 tatatacatc ctgccgggag catttttgct gcgattcaaa aaaaatgaga gtctcctaaa
     5881 gtttccttcg ataatatcaa tgcttaatat caatcttctt tttaatccac taaatcttcc
     5941 gggaatggga agtggtgtgc ttgaagatat tatgagtatt ccagatagtt cactta
//
LOCUS       DAJTRD010000168         5981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTRD010000168 DAJTRD010000000
VERSION     DAJTRD010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.168"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(105..1814)
                     /locus_tag="OO315_003899"
     CDS             complement(105..1814)
                     /locus_tag="OO315_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5892609.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2246..2965)
                     /locus_tag="OO315_003900"
     CDS             complement(2246..2965)
                     /locus_tag="OO315_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5892610.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
     gene            complement(3090..3646)
                     /locus_tag="OO315_003901"
                     /pseudo
     CDS             complement(3090..3646)
                     /locus_tag="OO315_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3630..3888
                     /locus_tag="OO315_003902"
                     /pseudo
     CDS             3630..3888
                     /locus_tag="OO315_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(3997..4575)
                     /locus_tag="OO315_003903"
     CDS             complement(3997..4575)
                     /locus_tag="OO315_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892611.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(4996..5233)
                     /locus_tag="OO315_003904"
                     /pseudo
     CDS             complement(4996..5233)
                     /locus_tag="OO315_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(5420..5849)
                     /locus_tag="OO315_003905"
                     /pseudo
     CDS             complement(5420..5849)
                     /locus_tag="OO315_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1661 a         1351 c         1057 g         1912 t
ORIGIN      
        1 tgccaattga ggtggttgcc tgaaagttgg cagttacccg gtcactacac cttgttggag
       61 tgaacatatc aaaaacaggt aattacacaa tttaaattac agtctcataa aaaatgatta
      121 aatctattta ttatttcaat aaacgttttt ttcatcttct ttgaaaattg ttctgctata
      181 gtatccggtt ccagattgtt ctcttttatt attctacaat actctaatat tgtttctttg
      241 tctctattta ctacagatac aaatacacca ggaataccat ctttattagt tgcggacagc
      301 atttcgacca atcgtcttgt ggtaaaacta tagtttttta gaatattaca aaaggcacgt
      361 attgtttcgg tatggccatt ataaaatgcc ataaaaagtc ctgatgtacc gttgggattt
      421 tttatccata ataattcatc tatatattcc tggtgagggg ggagtatttt tagtatatct
      481 ccatacgcca taatggcctt tgtgtgccca ttttgaagtg ccataaacaa cccatgcttt
      541 actttcaata attctttaat ttgttcacta ctaagcattt tcttattaag cagacttccg
      601 aacatacgaa tactatctgc atgtccattc tgcaatgcaa aaaataaccc aggtaaatca
      661 tggctgtctt tcgcagctaa tagatataat ctcttatcgg aagttaacgg ggttttaaag
      721 actatttccc cataggcttt tattgtgtct acatgtccgt tcataaaaga catgcataaa
      781 cccggcacat tgtttgcccc taccgcagca agcaatgtaa gtattgtttc ttgactaaga
      841 ttgcacatat ttaatatctt accatatact tgaataatgt ctgaatatcc gttttgaagt
      901 gctatataaa atgcaggagt accatcagag cttctcgtgg ccagaaggtt caccagttta
      961 gattgagaaa tttcaccatt tttgtatttt tccatcagta taaataatac ttctgtcaga
     1021 ttctttgtta tcgcatgata tataactctg gaattgatca attcatctgg taaaagtttc
     1081 actgtgtttg ttagagggga attatgccta tcttcaaata ttacctgccc gggttgctcc
     1141 agaccataaa ttttccaata aaaatcatca aaaaaatcct tagcggataa ttgactcagt
     1201 tcgaaatggc tatcacaagt aaatacagta cgtttatgag taacagttcg attaggatcg
     1261 aaaaactgaa caacccattt ttcactttct tgtacgcgct ttattttcaa ccctaacgcc
     1321 atttggtggt ttaatgtgca tgctgataat gttctgacaa agtctccatt ttttttcatg
     1381 ttataaaaaa aattagtaat aactgttccc cactttttat tttccacaaa ataaatataa
     1441 gctggtgatt ttgaaaaatc ttccgttttt tccaaacata cattttttgc aatagcatca
     1501 ggggtgctaa gaagctgata gtttaccttc ccatttgttt ccataaaatt catttcccaa
     1561 taggaagcta ggtgacggca aactatttgt cttccatata tatcttttgc cactccattt
     1621 atatttacct ccacatttct cttttctgaa aaataaggtg ctgttttatt cttaaactga
     1681 attagttcac tggagtttga gaccctcata atagaacttg ttattgttct attatctctg
     1741 ttaacagaca tatctgttga taacgaattt tttgttgaaa atatactaga tgaaaatgtt
     1801 ttatttaacg gcatagaaga caaccataga gatgttataa tactattaga ttgcagttac
     1861 taatagtaaa agcctcctta caaggaggta gcttattcag aatgctccta taagatagcg
     1921 agctcgtcaa cgatacagat gatctgatta tattgtgcaa aaggagaaca ggaaaatagg
     1981 cactggaaaa gatgaaggcc tgatgggagc attaactaat gcaaaatccg acaggttaat
     2041 atcccgaaaa gcaatcaaac gactcaaaca ggagatgcac gctgaaatgg tggacaactg
     2101 ttcggatttc agatgagtag ctcagcagtt tatgagccga atgctctgtc acgggcagga
     2161 atcaggctgc atacgcagtg ccaaatctaa tcgtagttat tctaaccagt tttcaagcta
     2221 caaggtggtg tagctggcat cttctctact ctatcatcaa acgataaaat ggttcctctc
     2281 gtaacatttg ctcttgcctt tcacttccat ctcgatcgct tcgtagttca ttacgatagc
     2341 tgacatattt ccagtcttca ggtctaacat ctgacgccgg atactccccg ggagcgattt
     2401 tgcttcttaa tagattagac tcgagacaca ttataaattg tcgtatttta tgaagtaata
     2461 aagcactata ttgcgagcat tcctgatctg attttacata tagcgtaaac tgagctccta
     2521 tccccacacg agattgctga gaaactcgat tcatatcagt tatcttccat ttatctatag
     2581 gactgtcctc tgaaaataat aaaccagaca aaatttggaa tgccagagga acttgctccc
     2641 tagctatact aatatgaaac ttgtccccaa caaaatcccc cttactctga ggattttcac
     2701 gacatgcatg aataaacaca tctccactat ggttcacaat ctggaatcct tggtacatga
     2761 cagaaaaaga ttgacatata tcagagtaag ttggcacgtt cgatgctact ttgaaatagt
     2821 acgctgggta ttgccgtatt tggttgtaca atatattgaa gttatttttc aatctttcat
     2881 tcgcagaaag catctgcggt atttcagacc tcacaacatt caaagaatct aaatttagtt
     2941 ttagacaggg cttttttatg ggcatagaaa acgtcctcta taaaatagat atattatctt
     3001 atattgattg tttatcatct tttataaaac gatacattat atgtaatgta tgtaagcgta
     3061 agggtcctct ccgaacatgg tgggaaggct tagaacgcca tattcagttt cccatagtgg
     3121 aacatcactc ccagcttaaa gcgctcccgg ttgcggtagc ccttggcttt tattctcagc
     3181 agcctgatct tgctgttcag tgcttccgca tttccgtttg atacgccatg gcgcatggcg
     3241 ttcaggatac cgtacaacct tttccctatc gttttagcca cattcttcat catggggacg
     3301 tcactgttgg ccgcagccat ctctgccagt cgttgcgtct ttcctcgctc catggtctgc
     3361 tccagatatc ctttgccagt tctttcagcg cccagcacag gctcgtcagc ttcatctgcg
     3421 cacgcagcca tatcagcttt tcctgcctgg attcggtcat ccatttatcg ctgtgctgcc
     3481 acaggaagcg agttcccttt gcctgacgac gactttcaac aggaagatgc ggatgttcat
     3541 tctggcgggt tttatccacc acctcacccg gttgtttcgc cacatgaaag cgatcgaagg
     3601 ctattttctc aaccgcattg ggtaagtgga tgcgcgctgc tcttagtttg ctgagccagt
     3661 ttagcattgc cttctacatg accgatgcct gagccggtat accgaacttt attgtcctca
     3721 accagtcatg ttataagcaa gaaatgcact cagcggatag agcgacataa tctgaacctg
     3781 cggacacatc ttaagcggct tacccgcaaa accatatgct tctcaaagtc tgatgatatg
     3841 cactacaaga tcatcggctg gtatctgaca atcaaccacc accactaaat ctgcgtcacg
     3901 acctgcaact gagtctggtt gaacaggctg aggccctgct gaagcaacgc gaacctctcg
     3961 actcttgttg gattttacca aatagttttt ctaaaattaa acacatttat ataagttctt
     4021 tgctgaacaa aacgcagccc gccatccttt ttcattcata aattcagcaa tggcagataa
     4081 aactttttca tcaagcatat aaaaaataat gccagtagac tcaatatgcc tcatacaaat
     4141 aaggccgcac atatccgtaa tatatttgtc aatgctgcta ccatttgcat ggtaatatgt
     4201 catgctatta tctgatgatt ttacatatgc agtatgacat atttttcctt cacgattaac
     4261 aaaactgaca aaaccacttt caccaggagt aatatataaa gatgaattta acgtgcgctc
     4321 actaatattc attaattgtt gaaattcaat ggaatagttt tcagttaatg tatctattat
     4381 cttggctttg tctttctttg ttatctcatt gtctttttcg gcaagaaaaa ttgccacttt
     4441 atgacatgca ttcagctcgt taattttacc tgataaaact gcttttctta ccacatcgtt
     4501 gaactcatcg atagtgtgtt ttttattata tatatcatga aaaatatagc ctaaagactt
     4561 aaacgagact ttcattttat attctcctta aatattaaca ggatatatat atcacatttt
     4621 cctctatatt ttcagaaacc agctatcatt ttccccatta ctggtacgtc gcttcagata
     4681 tttccgcagc aagcgataac cctgcgcact atgaggatgc cattcattat aatgtttgaa
     4741 caccttcgcg agactacttt actgctgtta acacgtcagg ttcgaacata acactgattt
     4801 aatcatattt catcgttttg acgaagtttt ctgccattcc attttgcacc gccgtgtgtt
     4861 tgggggcaag gccgaccatc tgtgtttcat tcgccagata taagtgatga gatggcattc
     4921 tccctccttc acggcagaag cattttgacc gtttagggga tgcggacgat tttcatgcct
     4981 gctaagatac tttcgttact gatgctgtcc gttttccacc atttcatcat cactgattgg
     5041 cgttgtgttt ttggtcagca ctccagcttt acgtttctct tttaaccggt aactctctcc
     5101 tttgatattc agcgtgtagc agccgatcta ggatcgctgt tgccagcacg tgatctccga
     5161 acatttctcc ccagtctgcg acccctttgt ttgacgtcag tatgatgctc gctttttcat
     5221 atcgacagtt catcgatagg aatttaaatc cccaaaagat taaaaaagca ccgaagaatg
     5281 cgcgtttatt caacacaacc tgatcatcaa gtaatcggta tagacactta atattgctta
     5341 cattgattga ttcaatgtat ctctgatggt tttttgctcc gttcccctca acatggacgc
     5401 aacttcccct ctgcgactct cagccgcacc accgtattcg agccagcggc tcatgcatca
     5461 taacctgctc tgccagacgg taacccctct tcagccccgt aaaccgcatc tgacctccgc
     5521 gcagctcgca cttcagcggg tcaaccttca gacactgctg atacatccct ctccttgtga
     5581 tttgcctcgc cgtttttctc actgtctccg ttatggcgta caccacatct tccagtaatc
     5641 gccgcttcac cggactcagg accccgtatc accatccgga accccttatc cgccacatgc
     5701 caggagaacc tttccataaa ctcgtcgcca ctcatcacaa ggttttcttc tcgttttgtc
     5761 cggtggctgt tgtaacgcag gccaatttca tcctgaccgg cataatgttc caggcggctc
     5821 atctgcaccg gctgcttctc cagccagtta agccatcggt ttccctgctg aaaaatatcg
     5881 gcaaaacggg gaagcatcag aaaggcggaa taacgccatc cggccagtga accgtaccgc
     5941 attcagggca atacatgccg tcggcgttga taccggcagg a
//
LOCUS       DAJTRD010000169         5867 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTRD010000169 DAJTRD010000000
VERSION     DAJTRD010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.169"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..266
                     /locus_tag="OO315_003906"
     CDS             <1..266
                     /locus_tag="OO315_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5892612.1"
                     /translation="ATLGRLMSLLSPFDVVIWMTDGWPLYESRLKGKLHVISKRYTQR
                     IERHNLNLRQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            263..526
                     /locus_tag="OO315_003907"
     CDS             263..526
                     /locus_tag="OO315_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5892613.1"
                     /translation="MSWSHYRPLLSHVAGFISIERFQSLATEGKMLSLSWWENEYAVL
                     QWKNHVLHAKAQQEGRESIFDFYKISIAHITREYSFKKDKDNV"
     gene            519..905
                     /locus_tag="OO315_003908"
     CDS             519..905
                     /locus_tag="OO315_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HCR5892614.1"
                     /translation="MFDVHVVLDNQIGQLALLGKTLGNKGIGLEGGGIFTVGDECHAH
                     FLVEQGKEAKIALEQAGLLVLAIRTPLIRKLKQEKPGELGEIARVLAENNINILVQYS
                     DHANQLILITDNDSMAASVTLPWAIK"
     gene            913..1599
                     /locus_tag="OO315_003909"
     CDS             913..1599
                     /locus_tag="OO315_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5892615.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(1577..2200)
                     /gene="tetR(B)"
                     /locus_tag="OO315_003910"
     CDS             complement(1577..2200)
                     /gene="tetR(B)"
                     /locus_tag="OO315_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5892616.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            2282..3487
                     /gene="tet(B)"
                     /locus_tag="OO315_003911"
     CDS             2282..3487
                     /gene="tet(B)"
                     /locus_tag="OO315_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5892617.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(3600..4193)
                     /gene="tetC"
                     /locus_tag="OO315_003912"
     CDS             complement(3600..4193)
                     /gene="tetC"
                     /locus_tag="OO315_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5892618.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            4326..4697
                     /locus_tag="OO315_003913"
     CDS             4326..4697
                     /locus_tag="OO315_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5892619.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
     gene            complement(4707..>5867)
                     /locus_tag="OO315_003914"
     CDS             complement(4707..>5867)
                     /locus_tag="OO315_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HCR5892620.1"
                     /translation="ELHLKRLNSLTLACHALLDCKTLTLTELGRNLPTKARTKHNIKR
                     IDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIREQKRLMVLRASVALHG
                     RSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVSDAGFKVPWYKSVEKLG
                     WYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKRLTKSNPISCQILLYKS
                     RSKGRKNQRSTRTHCHHPSPKIYSASAKEPWVLATNLPVEIRTPKQLVNIYSKRMQIE
                     ETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWLAGVHAQKQGWDKHFQA
                     NTVRNRNVLSTVRLGMEVLRHSGYTITREDLLVAATLLAQNLFTHGYALGKL"
BASE COUNT         1615 a         1096 c         1298 g         1858 t
ORIGIN      
        1 tggcgacgct ggggcgtctt atgagcctgc tgtcaccctt tgacgtggtg atatggatga
       61 cggatggctg gccgctgtat gaatcccgcc tgaagggaaa gctgcacgta atcagcaagc
      121 gatatacgca gcgaattgag cggcataacc tgaatctgag gcagcacctg gcacggctgg
      181 gacggaagtc gctgtcgttc tcaaaatcgg tggagctgca tgacaaagtc atcgggcatt
      241 atctgaacat aaaacactat caataagttg gagtcattac cgaccactat taagtcatgt
      301 agctggtttt atttcaattg aacgttttca aagtctagct acagaaggaa aaatgttatc
      361 gctatcttgg tgggaaaacg aatacgcagt tctgcaatgg aaaaatcatg ttttacatgc
      421 gaaagctcaa caagaagggc gagagtcaat atttgatttt tacaaaatta gtattgctca
      481 tattactcgc gaatattcat ttaaaaagga caaggataat gtttgatgtt cacgttgttt
      541 tagataatca aataggacaa ttagcattac taggaaaaac attaggtaat aaaggtattg
      601 gattggaagg gggagggata tttacggttg gtgatgaatg ccatgctcat tttcttgttg
      661 aacaaggaaa ggaagctaaa atagcgctag agcaagctgg actgttagta cttgcgatcc
      721 ggacaccatt aattcgtaag ttaaaacagg aaaaaccggg ggaacttggc gaaatagcac
      781 gagtattggc ggagaataac attaatattt tagtgcaata cagtgaccat gctaaccaac
      841 tgatattaat aacggacaat gatagtatgg ctgcatctgt tacgctccct tgggcaataa
      901 agtgaacttg cgatggctaa tttaatacga aaagaggtta cctttgagtc ctcaatagcc
      961 gcgatagggg cggctatgtc tgacatttca cgagttaaaa tactcagtgc tttgatggat
     1021 gggcgagctt ggacggccac tgagctaagt tctgtggcga atatatcagc ttcaacggcg
     1081 agcagtcatt tatctaaatt attagattgc cagctaatca cagtagtagc tcaaggcaag
     1141 catcgttatt ttcggctagc aggaaaagat attgctgaat tgatggaaag tatgatgggg
     1201 atctccttaa accatggcgt acatgccaaa gtttccacgc cagtgcattt acgaaaagca
     1261 cgtacttgct atgatcattt agctggcgaa gttgccgtta agatctatga ttccctttgt
     1321 caacagcaat ggatcactga aaatggttca atgatcacat taagtggtat tcaatatttt
     1381 catgaaatgg gaattgacgt tccttccaaa cattcacgta aaatctgttg tgcgtgttta
     1441 gattggagtg aacgccgttt ccatttaggt gggtacgttg gagccgcatt attttcgctt
     1501 tatgaatcta aagggtggtt aactcgacat cttggttacc gtgaagttac catcacggaa
     1561 aaaggttatg ctgcttttaa gacccacttt cacatttaag ttgtttttct aatccgcata
     1621 tgatcaattc aaggccgaat aagaaggctg gctctgcacc ttggtgatca aataattcga
     1681 tagcttgtcg taataatggc ggcatactat cagtagtagg tgtttccctt tcttctttag
     1741 cgacttgatg ctcttgatct tccaatacgc aacctaaagt aaaatgcccc acagcgctga
     1801 gtgcatataa tgcattctct agtgaaaaac cttgttggca taaaaaggct aattgatttt
     1861 cgagagtttc atactgtttt tctgtaggcc gtgtacctaa atgtactttt gctccatcgc
     1921 gatgacttag taaagcacat ctaaaacttt tagcgttatt acgtaaaaaa tcttgccagc
     1981 tttccccttc taaagggcaa aagtgagtat ggtgcctatc taacatctca atggctaagg
     2041 cgtcgagcaa agcccgctta ttttttacat gccaatacaa tgtaggctgc tctacaccta
     2101 gcttctgggc gagtttacgg gttgttaaac cttcgattcc gacctcatta agcagctcta
     2161 atgcgctgtt aatcacttta cttttatcta atctagacat cattaattcc taatttttgt
     2221 tgacactcta tcattgatag agttatttta ccactcccta tcagtgatag agaaaagtga
     2281 aatgaatagt tcgacaaaga tcgcattggt aattacgtta ctcgatgcca tggggattgg
     2341 ccttatcatg ccagtcttgc caacgttatt acgtgaattt attgcttcgg aagatatcgc
     2401 taaccacttt ggcgtattgc ttgcacttta tgcgttaatg caggttatct ttgctccttg
     2461 gcttggaaaa atgtctgacc gatttggtcg gcgcccagtg ctgttgttgt cattaatagg
     2521 cgcatcgctg gattacttat tgctggcttt ttcaagtgcg ctttggatgc tgtatttagg
     2581 ccgtttgctt tcagggatca caggagctac tggggctgtc gcggcatcgg tcattgccga
     2641 taccacctca gcttctcaac gcgtgaagtg gttcggttgg ttaggggcaa gttttgggct
     2701 tggtttaata gcggggccta ttattggtgg ttttgcagga gagatttcac cgcatagtcc
     2761 cttttttatc gctgcgttgc taaatattgt cgctttcctt gtggttatgt tttggttccg
     2821 tgaaaccaaa aatacacgtg ataatacaga taccgaagta ggggttgaga cgcaatcgaa
     2881 ttcggtatac atcactttat ttaaaacgat gcccattttg ttgattattt atttttcagc
     2941 gcaattgata ggccaaattc ccgcaacggt gtgggtgcta tttaccgaaa atcgttttgg
     3001 atggaatagc atgatggttg gcttttcatt agcgggtctt ggtcttttac actcagtatt
     3061 ccaagccttt gtggcaggaa gaatagccac taaatggggc gaaaaaacgg cagtactgct
     3121 cggatttatt gcagatagta gtgcatttgc ctttttagcg tttatatctg aaggttggtt
     3181 agttttccct gttttaattt tattggctgg tggtgggatc gctttacctg cattacaggg
     3241 agtgatgtct atccaaacaa agagtcatca gcaaggtgct ttacagggat tattggtgag
     3301 ccttaccaat gcaaccggtg ttattggccc attactgttt gctgttattt ataatcattc
     3361 actaccaatt tgggatggct ggatttggat tattggttta gcgttttact gtattattat
     3421 cctgctatcg atgaccttca tgttaacccc tcaagctcag gggagtaaac aggagacaag
     3481 tgcttagtta tttcgtcacc aaatgatgtt attccgcgaa atataatgac cctcttgata
     3541 acccaagagg gcatttttta cgataaagaa gatttagctt caaataaaac ctatctattt
     3601 tatttatctt tcaagctcaa taaaaagccg cggtaaatag caataaattg gcctttttta
     3661 tcggcaagct cttttaggtt tttcgcatgt attgcgatat gcataaacca gccattgagt
     3721 aagtttttaa gcacatcatc atcataagct ttaagttggt tctcttggat caatttgctg
     3781 acaatggcgt ttaccttacc agtaatgtat tcaaggctaa ttttttcaag ttcattccaa
     3841 ccaatgatag gcatcacttc ttggataggg ataaggtttt tattattatc aataatataa
     3901 tcaagataat gttcaaatat actttctaag gcagaccaac catttgttaa atcagttttt
     3961 gttgtgatgt aggcatcaat cataattaat tgctgcttat aacaggcact gagtaattgt
     4021 tttttatttt taaagtgatg ataaaaggca cctttggtca ccaacgcttt tcccgagatc
     4081 tcatctattg aaacagcttg atagcctttt tcaacaaaca atattcgtgc tgagttaacc
     4141 agtgattgat aggtactctt aaaattttct tgttgatgat ttttattttc catgatagat
     4201 ttaaaataac ataccgtcag tatgtttatg gtatcatgat gatgtggtcg tgacaatctt
     4261 aagaacattt aggttatttt atgtatattg aacagcattc tcgctatcaa aataaagcta
     4321 ataacatcca attaagatat gatgataagc agtttcatac aacggttatc aaagatgttc
     4381 tattatggat tgaacataat ttagatcagt ctttactgct tgatgatgtg gcgaataaag
     4441 cgggttatac caagtggtat tttcagcggc tgttcaaaaa agtaacaggg gtcacactgg
     4501 ctagctatat tcgtgctcgt cgtttgacga aagcggctgt tgagttgagg ttgacgaaaa
     4561 aaactatcct tgagatcgca ttaaaatatc aatttgattc ccaacaatct tttacacgtc
     4621 gatttaagta catttttaag gttacaccaa gttattatcg gcgtaataaa ttatgggaat
     4681 tggaggcaat gcactgagag atcccctcat aatttcccca aagcgtaacc atgtgtgaat
     4741 aaattttgag ctagtagggt tgcagccacg agtaagtctt cccttgttat tgtgtagcca
     4801 gaatgccgca aaacttccat gcctaagcga actgttgaga gtacgtttcg atttctgact
     4861 gtgttagcct ggaagtgctt gtcccaacct tgtttctgag catgaacgcc cgcaagccaa
     4921 catgttagtt gaagcatcag ggcgattagc agcatgatat caaaacgctc tgagctgctc
     4981 gttcggctat ggcgtaggcc tagtccgtag gcaggacttt tcaagtctcg gaaggtttct
     5041 tcaatctgca ttcgcttcga atagatatta acaagttgtt tgggtgttcg aatttcaaca
     5101 ggtaagttag ttgctagaac ccatggctcc tttgccgacg ctgagtagat tttaggtgac
     5161 gggtggtgac aatgagtccg tgtcgagcgc tgattttttc ggcctttaga gcgagattta
     5221 tacaatagaa tttggcatga gattggattg cttttagtca gcctcttata gcctaaagtc
     5281 tttgagtgac tagatgacat atcatgtaag ttgctgatag gtttccagtt ttccgctcct
     5341 aggtctgcat attgtacttt tcctcttact cgacttaacc agtaccaacc cagcttctca
     5401 acggatttat accatggcac tttaaagcca gcatcactga caatgagcgg tgtggtgtta
     5461 ctcggtagaa tgctcgcaag gtcggctaga aattggtcat gagctttctt tgaacattgc
     5521 tctgaaagcg ggaacgcttt ctcataaaga gtaacagaac gaccgtgtag tgcgactgaa
     5581 gctcgcaata ccataagtcg tttttgctca cgaatatcag accagtcaac aagtacaatg
     5641 ggcatcgtat tgcccgaaca gataaagcta gcatgccaac ggtatacagc gagtcgctct
     5701 ttgtggaggt gacgattacc taacaatcgg tcgattcgtt tgatgttatg ttttgttctc
     5761 gctttggttg gcaggttacg gccaagttcg gtaagagtga gagttttaca gtcaagtaat
     5821 gcgtggcaag ccaacgttaa gctgttgagt cgttttaagt gtaattc
//
LOCUS       DAJTRD010000170         5807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTRD010000170 DAJTRD010000000
VERSION     DAJTRD010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.171"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            65..583
                     /gene="mddA"
                     /locus_tag="OO315_003915"
     CDS             65..583
                     /gene="mddA"
                     /locus_tag="OO315_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5892621.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGHWLDLTFMQL
                     QLDERTEPDAIG"
     gene            580..1029
                     /locus_tag="OO315_003916"
     CDS             580..1029
                     /locus_tag="OO315_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5892622.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(1030..1263)
                     /locus_tag="OO315_003917"
     CDS             complement(1030..1263)
                     /locus_tag="OO315_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5892623.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="OO315_003918"
     CDS             complement(1349..1522)
                     /gene="ortT"
                     /locus_tag="OO315_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5892624.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1717..1812
                     /gene="yncL"
                     /locus_tag="OO315_003919"
     CDS             1717..1812
                     /gene="yncL"
                     /locus_tag="OO315_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5892625.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(2214..3023)
                     /locus_tag="OO315_003920"
     CDS             complement(2214..3023)
                     /locus_tag="OO315_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5892626.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(3233..4657)
                     /gene="patD"
                     /locus_tag="OO315_003921"
     CDS             complement(3233..4657)
                     /gene="patD"
                     /locus_tag="OO315_003921"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR5892627.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4679..5473)
                     /locus_tag="OO315_003922"
     CDS             complement(4679..5473)
                     /locus_tag="OO315_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5892628.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5463..>5807)
                     /locus_tag="OO315_003923"
     CDS             complement(5463..>5807)
                     /locus_tag="OO315_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5892629.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1522 a         1474 c         1288 g         1523 t
ORIGIN      
        1 gttgcttcgg gctgttttcg atagtattga taacatcatc atcattattt tattgaggcc
       61 agacatgtcc atccgttttg cccgcaaagc cgactgtgct gccattgcgg aaatttataa
      121 ccacgccgtg ttgtatacgg cggctatctg gaatgaccaa acggtggatg cagataaccg
      181 cattgcctgg tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga
      241 tggcgtagtt acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca
      301 taccgtggaa cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa
      361 attgttaagt cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg
      421 gatcgaatcg caaaatcagg cttcgctgca tctgcaccag tcgctgggat ttgtcgtcac
      481 cgcacaaatg ccgcaggtag gcactaaatt tggtcattgg ctggatctga catttatgca
      541 gttgcaactc gacgagcgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt
      601 gcttttttga ttgccgccgg aattggtctg gtagtccaga acacgctgat ggcgcgcatc
      661 acccagacct cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg
      721 ctctttgttt ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc
      781 agcgtgcgct ggtggacact tatacctggc ttgctgggtt cgttttttgt cttcgccagt
      841 atcagcggat atcagaatgt gggagcggcg acaactattg cggtactggt cgccagccag
      901 ctgattggcg gtctggtgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg
      961 ttcggcccaa tatgtggcgc aattttactt gtggttggcg cctggttggt ggccagacgc
     1021 tcattttaat cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc
     1081 ttctttatgc ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta
     1141 gcgatcttcc cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg
     1201 ggcaatgacg ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga
     1261 catactttct ccttagccac ttgtgaaatt tatcgattaa tccaaatgat ataagtctgc
     1321 gtggtgccgt caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga
     1381 aactcatcgg ccatgtggcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat
     1441 cgtgagaaag aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa
     1501 gcatatgttg atagagaaac atgtacgggc gtccagccat atgaataaaa cgcgcgcaaa
     1561 tatgcgccat tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa
     1621 cagcaacttg cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta
     1681 ctatacacat aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta
     1741 gtactgataa atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc
     1801 tggtttattt gatgtagtga ttttgcacgc agcacgtact gtcagagaaa tcaactctct
     1861 gacagtacgc aagatcttct catactctcc aagtttgcaa cattatcctt aatatttact
     1921 tctttttgct ggcatctaca ttgaaatgag tagaaaacat ccccctactg tcgtctatac
     1981 aaacagctta ctgattcaaa cggaatcttg ttttcacatt tttctatcac gaatacttta
     2041 tacgcgcggg agtctataaa tgctttatac tttcaaggaa taacgtaaat ataaaaagat
     2101 ggcgtaaagt atatgcatgg caggatgttt gcaacctcct tgatgacgct gccccattga
     2161 aattgcaaac cccgccctgg ttatcttgcc catttatcat gcaaaacaga taattatagg
     2221 cctctgaata cctcagaaag attgaatttt ttatagtccc tctcttgaat atttccattt
     2281 gatatcaatc gattcatctc ttcaacaaaa taatttatat catcacactt attattatcc
     2341 ggaagatctg agtagttata attccaccat tttattctca acagttcatc aataacattt
     2401 gaatcaaacc taaatctgat tatttttgca ggaactcctg caactatggc atatgggggc
     2461 acatctttgg taacaacgga attggcagca ataacagcac catcacctat tgcgatgcca
     2521 cctttcaata caacatcatt tcctatccag acatcatttc ctataatcgg attttcaggt
     2581 ttggtaatga atggtttcaa tgtccagcta cgttcaaatt cactcatagc aaacttatca
     2641 aattcaccgt tataagtcat catgtgagta gtaaaacgat tcaaaggatg atttccaccc
     2701 attattttta catttgatgc aatactacaa aatcgaccta tacgtatatt ccctgaaaaa
     2761 tggcaggtcg gaaatgaaaa agcgccgaca ctggagaaat ttttcacagg cattgttgaa
     2821 tattcttcag caataacatt atcttcaatt gttatcttgt caccttttga gaaaagaaat
     2881 aaattccttt ctttatacga cttcttcccc ttatgattta aaaagatttt attatccata
     2941 caaaactcaa agtgtttttt cgtccatctg aaggtaaggg ttttattttt atcttccgtg
     3001 aatttgtaca attgaacttt cattgttaat acttctactt ataaagtatt agtttaataa
     3061 cgatactcta aagccttatg ctaatcaact gtgacaggat gaaaaatcgc catcaaacat
     3121 ttagcctaat ttttacgcca gttattaatg taatcattct aatacttaac cgccattaaa
     3181 ctcagaacct aaaacgatag tggccgtcct ataacagcca ctaacatcta acttaatgtt
     3241 taaccatgac gtggcggacg acggtgtaat cctccagccc ataaagtgac atatccttgc
     3301 cgtaaccaga aagtttctgc ccaccgtgcg gcatttcact taccagcatg aaatgggtat
     3361 tgacccaggt acaaccatat tgcagccgtg cgctgacgcg atgcgccctg cccacatctt
     3421 tcgtccatac cgaagatgca agtccgtact ggctgtcatt cgcccagttc accacctgtt
     3481 cttcgttgtc gaagggcgta acactcacta ctggaccaaa tacctctttt tgcacgatgg
     3541 catcgtcctg taatgcgcca gccagcagcg tcggcgcata gtaatagcca ttacccttgc
     3601 gcttttcacc gccagtgatc actttgatgt gccctgtcgc tttcgcctct tctactgcct
     3661 tgctgacgcg ttcgagatgc gccagcgagc ttaaaggtcc aagctccgta gactcgtcat
     3721 ccggcgcacc agattttaac gttgccaccg cagcacccag tttttccacc agcgtatcgt
     3781 aaatgccttt ttgcgcgtag atccgacaag ccgcagtaca atcctgtcca gcattgtaat
     3841 agccaaatgt acgtacacct tcgaccactg cttcaatatc cgcatcatca aaaacaatca
     3901 ctggcgcttt gccaccaagt tccatatgag tacgcttaat ggacgacgcg gtatggctga
     3961 tgatgtgctc gccggtggcg atagagcccg tcagcgacac catccgcact ttgggatgac
     4021 cggtcagcgg atcacccacc gttttgcctc tgccaaacag tatgttaatc acgcctgccg
     4081 ggaagatatc tttcgccagc tctgccaact tcaacgcggt cagcggggta atttctgatg
     4141 gtttaagcac tacgcagttc cctgccgcca gcgccggagc aagtttccac gcggccatca
     4201 tcagcggata attccacggt gcgatagaag ccacgacccc caacggatcg cgacggatca
     4261 tcgaagtatg accttcaaga tattcacctg ccgccagacc attcagacag cgcgccgcac
     4321 ccgcgaaaaa gcgaaaaaca tcgacaatcg ccgggatttc atcattgaac gcactatgca
     4381 gcggtttgcc acaattacgg gattccagtt cggcaaaaac ctgaccattt tcttcgataa
     4441 catccgccag tttcagcagg cattccgcac gcgctttcgg tgtggtttgc ccccattcag
     4501 caaatgctgc atctgccgca cgcacagcag catcgacctg ctctgcggat acctcggcaa
     4561 tctccagtaa aacgtccccc gttgccggat tatagacagg ctgtttttcc ccttcgccgc
     4621 taacccgttc tccgttaatc agtaacttat gttgcatagc ttattcctgt atcagtggtt
     4681 attgaccatt gtcgccttcg cgggttagcc accaggcccc caggatcggc aaggttgtta
     4741 ccaacataac cagcagtgcc accacgttgg ttaccggtac atcacgcggt cgcccaagct
     4801 gattgagcaa ccacaacggt aacgttcgtt catgacctgc cgtaaaggtc gtaacgatga
     4861 tttcatcgaa cgacaaggca aacgccagca ttcctcctgc cagtaacgcc gaactgagat
     4921 tcggcaacac tacgtagcgg aaggtttgcc agccattggc cccaagatcc attgacgcct
     4981 caaccagact ccacgaggtg cggcgaaaac gggcgatgac attgttaaac accaccacta
     5041 cgcaaaaagt cgcatgaccg accacgatgg tgaaaaatcc cggctccaga ttgatggttt
     5101 taaaggcggt taatagcgcc agaccagtga caatgcccgg cagcgcaatg ggcagcagca
     5161 ataacagcga aatggcgttt ttgccgaaaa agtctcgtct ccacagcgcg gcagctgcca
     5221 gcgtccctaa caccagcgca attaatgtcg ccagcgccgc cactttaagt gacagcgtca
     5281 cggcatcaag aatatcacta cgctgtgctg ccacgctaaa ccagcgcagc gtcaggccct
     5341 gcggtggaaa actaaacgcc gcatcttcag tgttaaacgc ataggcggcg attatcagga
     5401 tgggaaaatg taggaaaaca acgccgcccc aggccgccag tttgaggaaa aacggtgcgc
     5461 gttcagagtg catcgaacgc tcccagacgt ttcacgaacg ccaggtacag tgcgatgaga
     5521 ataatcggca ccagggtgaa tgccgccgcc atcggcatat tgccaatcgc cccctgctgg
     5581 gaataaacca tattgccgat aaaatatcct ggaggaccaa ccagctgcgg gacgataaaa
     5641 tcgcccagtg tgagtgagaa ggtaaagata gagccagcgg caatacccgg gattgccagc
     5701 ggcagcacca catagcgaaa ggtttgtcgt ggacgtgcgc cgagatcagc cgacgcctgc
     5761 aacaatgacg gcggcaaacg ctcaagcgcc gcctgaacgg gcaggat
//
LOCUS       DAJTRD010000171         5738 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTRD010000171 DAJTRD010000000
VERSION     DAJTRD010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.172"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..241
                     /locus_tag="OO315_003924"
     CDS             <1..241
                     /locus_tag="OO315_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892630.1"
                     /translation="FGIFYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPR
                     TVGKREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(238..1179)
                     /locus_tag="OO315_003925"
     CDS             complement(238..1179)
                     /locus_tag="OO315_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5892631.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1191..1680)
                     /locus_tag="OO315_003926"
                     /pseudo
     CDS             complement(1191..1680)
                     /locus_tag="OO315_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1684..2904)
                     /locus_tag="OO315_003927"
     CDS             complement(1684..2904)
                     /locus_tag="OO315_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5892632.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2908..3651)
                     /locus_tag="OO315_003928"
     CDS             complement(2908..3651)
                     /locus_tag="OO315_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5892633.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3542..5008)
                     /locus_tag="OO315_003929"
     CDS             complement(3542..5008)
                     /locus_tag="OO315_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5892634.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTALGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(5008..5241)
                     /locus_tag="OO315_003930"
     CDS             complement(5008..5241)
                     /locus_tag="OO315_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5892635.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5229..5708)
                     /locus_tag="OO315_003931"
     CDS             complement(5229..5708)
                     /locus_tag="OO315_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892636.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1225 a         1618 c         1436 g         1459 t
ORIGIN      
        1 ctttggcatt ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt
       61 gcgcacgagt gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa
      121 actcgcgccg agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg
      181 catcattttt tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta
      241 aatgccatca acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc
      301 gtagtaagtg acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac
      361 cagcgggtag cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc
      421 cacaccacgg cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg
      481 atagctgatg gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac
      541 gatgatgctg gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac
      601 agagtaacca gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc
      661 gtccggtgtt gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt
      721 aacaaggcct ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat
      781 ctgccatttc agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg
      841 agcagcctgc aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt
      901 accggatttg ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca
      961 gttttttcca ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct
     1021 ggaaatgtca tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta
     1081 ccccaccagt ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc
     1141 accagagctg tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc
     1201 caggaaattt ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc
     1261 accacgtttt tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat
     1321 tcatcagtgg agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac
     1381 ggtcacgtag ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg
     1441 aaccatgtcc ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc
     1501 tgcggtatca ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag
     1561 gccataggca gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg
     1621 cgggcgagag acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat
     1681 tttttaccct ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact
     1741 ggtcatgccc atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt
     1801 ggcaacctca gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat
     1861 atccgcatca ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg
     1921 tttgaacgcg gtcggtttca tcttacggct cagatcgata cccgggacga taacttcggc
     1981 acgccaggct gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt
     2041 cggattatcg tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag
     2101 cagggctttc agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat
     2161 ctctggtgct ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc
     2221 ctgcggcaaa tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt
     2281 catggtgtcg gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt
     2341 ttgatttgcc attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg
     2401 gacaagagcc acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg
     2461 ctcgtactcc gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat
     2521 ggcgctttcg tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc
     2581 gcgccggaca ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag
     2641 ccgcacattc ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga
     2701 ggcaagcgtg gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa
     2761 cttatcgggt ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc
     2821 tatcggcacg tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag
     2881 cctggtctca aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg
     2941 aacaaacacg atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat
     3001 gccgtccaaa gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga
     3061 atctcggaca tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat
     3121 cgctcgggcc atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc
     3181 ggccctagac ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg
     3241 accaccggtg acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc
     3301 ctcgagaggc agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg
     3361 gcccacagga gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg
     3421 ccgccaaact ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt
     3481 cgcccagggg gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact
     3541 gtcatttgaa ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg
     3601 taggctggtt cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc
     3661 cgatgccggg cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc
     3721 ctatgccggt tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca
     3781 gaccatcacc cagcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat
     3841 cgacatcaga catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct
     3901 tgccaagttc cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc
     3961 tctcctgcaa cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa
     4021 atcctttcgg ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct
     4081 gttctgcaaa ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa
     4141 acttatcccg cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt
     4201 cgatattctt cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct
     4261 tctccacgct gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag
     4321 cgctgtcgaa cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt
     4381 tctcggtcat cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt
     4441 gatgaatgcg ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt
     4501 tacccaggtc cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc
     4561 ggtcgagcgg gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg
     4621 tcattggtgc ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc
     4681 atttcagcac gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa
     4741 tgccacgatc ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat
     4801 ccgggatagc atcgactgct tcgccgatga tccaggatga acgataggac cattccacca
     4861 gcatgcggtt acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg
     4921 tctgcatccc tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc
     4981 gccgcagggc ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc
     5041 cagcgatgta tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg
     5101 tatctcgcgt tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa
     5161 atcacgatga ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc
     5221 tatgctgatc agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt
     5281 tctgaaacgg tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg
     5341 atgccgttgt actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa
     5401 ttcacacgtc gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta
     5461 gcaccggcag acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc
     5521 gccgcctgat aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg
     5581 tcggcccaga aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg
     5641 tcagccacgt caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc
     5701 gcatccactg cggcttctat ccaagacagt ttgatgat
//
LOCUS       DAJTRD010000172         5686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTRD010000172 DAJTRD010000000
VERSION     DAJTRD010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.173"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            86..496
                     /locus_tag="OO315_003932"
                     /pseudo
     CDS             86..496
                     /locus_tag="OO315_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            528..1142
                     /gene="istB"
                     /locus_tag="OO315_003933"
                     /pseudo
     CDS             528..1142
                     /gene="istB"
                     /locus_tag="OO315_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1311..5405)
                     /gene="sepA"
                     /locus_tag="OO315_003934"
     CDS             complement(1311..5405)
                     /gene="sepA"
                     /locus_tag="OO315_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5892637.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1401 a         1424 c         1353 g         1508 t
ORIGIN      
        1 gcttattatg cttccatcgg aaacgatgat ctggcagcct gagttcacag ataaaacact
       61 ctccaggaaa cccggggcgg ttcatgccgt caggcagcag cgaagcaaag cgttactgac
      121 gtcgctgaat gaatggatgg tggagaagaa tggtacgctg tcgaaaaaat ccagactggg
      181 cgaagcgttc agctatgtac tgaatcagtg ggatgccctc tgttattaca gtgatgacgg
      241 tctggcggag gcggacaata acacagcaga aagagcgctt cgtaccgtct gtctcgggaa
      301 aaagaattac atgttcttcg gcagcgatca tggaggcgat cgcggagctc tgctgtacgg
      361 actaatcggg agctgtcggt tgaacggtat cgatccagaa gcgtatctgc gccatatcct
      421 gagcgtactg ccggaatggc cttccaaccg agttgacgaa ctgctgccat ggaacgtagt
      481 tctcaccgat aaataagcgt caatacggcc ttccgttgac gcttacagaa ctcctgtggc
      541 gcgagagtga aaaactgcgt cgttataaaa aggaggcccg gttgccagtt gccaaaacgc
      601 taagcgaata cgacttcatt cagttgccgg aactgaatgg agctcagttc cagcaactct
      661 gtgaaacgac agactgggtt gatgctggag aaaacgttct gctgttcggg gccagcgggt
      721 tggggaaaag ccatctggcg gcagcgatcg tggatggcgt agtaggccag ggctaccggg
      781 cccggttcta cagcgcagga gagctgttgc aggaactacg taaagccaga gctcagttga
      841 aactgaatga gctgctactg aaactggatc gctaccgggt gatagtggtg gatgatcttg
      901 gctatgtcaa acgcgacagc gccgaaacgg gtgtgctgtt cgagttaata gcgcatcgct
      961 atgaacgtgg gagcctggtg ataaccagta accatccgtt cagcatgtgg ggcagcatct
     1021 tcgtggatga gactatggcg gtggcggcag cagaccgact gatccatcac ggatatatgt
     1081 tcgaactgaa aggggaaagc tacaggaaaa agacagcgaa ggcagtaaca agcgtgactt
     1141 gatgtcgcac tgaaggggcg gccagtatag ttggcgccag tcggcaaaac tagttgacgt
     1201 ctaatacctg gacggtcact tctgagaaaa ggcatttaca cagaatctta aacaggctca
     1261 cggagttctt tcagaaaaag gcctgccgaa tggcagacct atcccattcg tcagaaaaca
     1321 taacggaagt tggcgtttat cgcattatcc acattatatt tcccgaacgc tgatttctcc
     1381 agctccagtc ccaaacgcat attatcctta atttccgcat tcatccctac cgtcatcagc
     1441 atcctgctat ctttttcacc ttcgaaacgt ttctctccgg aagcatcctg cagaaccgtt
     1501 tctccgttcg ccagcaggtc gaactgataa cccagcccgg ctcgagctgt gattttccag
     1561 tcgtctccgg agaaggctct tcccacgtca acaccagtac ggccaatcag tgggttataa
     1621 tccttgtctt tcatgctcag agccattccc cggtcttccc agctaaaagc ttttcctgat
     1681 acagaaccgt aaaccagctc tatctgtggc tccacccagg actctttcgt caggtggtaa
     1741 cgataaccaa cttcagcacc ggcataccag gaatgagagc tgtagtcttt tgttcccagt
     1801 gaggcaaaat tcgccgtgtg ctgattatca tgatggagat atttaccaat caggtcaaaa
     1861 taagctccgg aattaaagag tgcagaggca tacagaccgc cacccacgga tttgtttttt
     1921 ccactgaatg cgtggctgct tgcattgctg tccgtatagg tcagcaatgc ccccgtaaat
     1981 aaatccacac cgtccagctc atgctttctg tcgacaccaa tctgaacgtg agtgtagtta
     2041 tcgctgtagt caccatctgc cgaaccggta ccattcatta tgcgtgccca gacaccggca
     2101 tccccctgag tatcccgcag gtcacccata cgtttgttca ggttattgac ctctgtaagg
     2161 aaggacttat aacctgatgc cataaagtct tttgcggctt tcgaggctcc ggcatcagcg
     2221 gtagtctgat atcctgtcag cacccatttt gtggcatcat ccgttttttc cgtgctgatt
     2281 actggcgtaa cgttactgaa accaatctgc tgcgtaccgg cagtgaacgt tttctcatca
     2341 ctccctttcg gtgcggtaat taacgtcacg ctcatttcac tggctggttt gtttaaaaag
     2401 tcaaccagga cgctattatt actgcctgac agtttgtctg tgacattcaa ctggtcagcc
     2461 cggctgttgt tggtatgcat caggaacgtg gtattatctg cagaaagctc ttttacttgt
     2521 aaagtggcaa agtgtttccc gtcccccagt tcaattcgcg agcccttagc cttcagtgca
     2581 ccggcctctg attttccgtc agagtaccac agagcattga tcatatttac cgtactctgt
     2641 gcccccgtaa tactgcctct cagaacagcg ttatatccct tcattaagga gctgaataac
     2701 ggagagtccg tcttccctgc atcagcatca ctgtctccca gattaatggt tgccgcagtg
     2761 gttcctgcat gaatatccga actcagatag cccatatttt taacagaaaa gctggcgtta
     2821 tcttcgaggt taatcccttc tgttgtagaa attacgggtg agtaataccc ctgtttctgc
     2881 gcagaaggca tccccgtcag ggtcagagaa gcattttctt ttacatccac agccccggtg
     2941 ctgaaaatac cgctctgagc cgtaagtttt gctccttttc cgagggtcag gctgctgttg
     3001 acaaagctgc caacacggtc aaaaacagca ttctgagagg tcacactcac ggtgctatca
     3061 taagcctcaa taccaccggt gaatctctcc atgatttgca gggatgattt attttccagc
     3121 gtaacatttc cgctgtaatc actctggtca ccaactgtgg ttgcagtggc actcccttca
     3181 acagaggaga ccgcattacc ggttccgtca ctcctgtcag taaagacacg gctacttccc
     3241 agaatgactg tacttccatt ggtggcgtta atatctccct gcatcttgcc gtttctggac
     3301 agatggaatg ttgccgcatc gagtttcagt ttttccagcg taaactgacg ggtttcccag
     3361 tcgctctggc tggaagaagt cgtcgttcca gcatgaataa ctggatgacc ctggaatatc
     3421 agcgtgccgg acttcttggt tatcgaacct tctggcaggt taactgaacc atcaagagcc
     3481 agtacatcct tacctgagag ctgcggaata ttcacatcca tattgccgtt tagttggccg
     3541 tgatacatat aaggctggct gctggcctca attttttgtt gcttcacagt atcaatggct
     3601 ttattacgat cttttccgac attctgccag acactgctgt tgttcagatc ggagaagaac
     3661 ggggaatatg aactcgcttt caggatgaaa tattgcttgg tagaactgtc ataatatagg
     3721 tctcccggcg ctcctctccc cccaaaaata ctgacagtat taaccggtac attgatgtca
     3781 ctggctttta atgtctgaag gtcgagtgtt accgttgatt ttttctcact gttattggta
     3841 atgatggccc cgtagtcagc cgcctgaaga cgggtaaagg tcagattatt accattcagt
     3901 tccagacgtc ctccacggta tccccacgaa atattatccg gattaacttg ctgtgagtca
     3961 gaaagcacaa ctgtcggacg accgctggca ataccaacag agctgaaagc ctgtaccttt
     4021 ccgtcagcat ccgcctgctg gttgagaata acggtaccgt cccccacctt gagtccgcct
     4081 gcattcacac cggtcccgtt tactgtcagg gtaccttcgc cagttttatg caggttatca
     4141 ccggcaactc cgttcacctg ccacagaacg tgggttcctt tatcggtgat aatcccgccc
     4201 cccatccatg tctggccgtt cagggcggac accctgtaat cgccggcaaa ctgcagatac
     4261 ccagctccct gattaacgga attctgcaga acgacctcgc cattattgcc ggtgaacagg
     4321 aggttttttc ctgcgttcag atcatttcct ttctttccat gcatatccca agtcgtgttt
     4381 ccctgagtca gtgtgccggt gccattagcc gcatcatatt tccactgcag tactccttca
     4441 ccagaggtgt aggctatagt tttgtcaaaa tcattttgcg gttgctgacc aaggaaatcc
     4501 tgagtggtga cgacccagtt atttccatag ttagttcctg aggaaagcac gccaatcagt
     4561 acccattttt tttgcaggga atcataagca aacagggggg agccgctgtc ccccatattc
     4621 agataattag ccagaggtcc attcgcggga ttaaaaatgt caccggtctg agctgtaatc
     4681 atctgtccgt tgttatagga gttcagcgcg ctgacggttc ccccaacaag aaatccgcca
     4741 ttggtataca cttgggtgcg attaccattt ttgtctttga cgtactgcat ccccccccca
     4801 aggcgataga attcagtaaa gcgtccgccg gcctgatagg caccgctcac agccccgatg
     4861 tcagacactt ctgccggcgc cacctctgtc accagtttac tcaggcggcg ggttttaata
     4921 tcaagaccgc tattattatt tgtaccaacc gcagtatagg tgtttttggc gtatccgaag
     4981 ctcatcgtgt cagaaccgct gacatgtttg gcggtaacca catactgagg ggagtaaagt
     5041 gtatggtctc ccggaggcag gctaccggta gtaatagtgg cactactgaa atcagccatc
     5101 ggggctttat caagtttacc gaccagattg ccctgcttgt cgtaaatgga aatattcgtg
     5161 gtccccggcg taaactgacc tttattctcg gcaaagtcgc ggaatatctg ataagggatc
     5221 tctgcactga ccgttgctga cagagctggc catgccgagg aaagtgataa agctgcaaca
     5281 gacgtaagaa taacccgacg tgaaagtctg cgatggcttt tgcaggtaac cctgcgggcc
     5341 agttcggaga cagcaatcag gcttttggtt atatggcaat acttaagata atatattttg
     5401 ttcatataga aacatcaacc taattttgac aaagattaat tacataggat gtagatggag
     5461 cagtccgaga ttctactaat atcaattatt aagcatctgt tttttagttg gtgtaatata
     5521 atttttatta aggtaagcgg aaaaacaacc aaaaaaaacg caagatgtta tttttttatc
     5581 attttgagcc agtttggctg gggggatact cttctcttaa gcttcttgct caactttaaa
     5641 aatagttatg ccagttcggt atcgataagg ggcagtttgg atatcg
//
LOCUS       DAJTRD010000173         5592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTRD010000173 DAJTRD010000000
VERSION     DAJTRD010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.174"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(1..279)
                     /locus_tag="OO315_003935"
     CDS             complement(1..279)
                     /locus_tag="OO315_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892638.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDGYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            220..405
                     /locus_tag="OO315_003936"
     CDS             220..405
                     /locus_tag="OO315_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892639.1"
                     /translation="MPAGRGGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO315_003937"
     CDS             complement(447..602)
                     /gene="hokD"
                     /locus_tag="OO315_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5892640.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1254..1670)
                     /locus_tag="OO315_003938"
     CDS             complement(1254..1670)
                     /locus_tag="OO315_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892641.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1697..1837
                     /locus_tag="OO315_003939"
                     /pseudo
     CDS             1697..1837
                     /locus_tag="OO315_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1837..2862
                     /locus_tag="OO315_003940"
     CDS             1837..2862
                     /locus_tag="OO315_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5892642.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2915..3004)
                     /locus_tag="OO315_003941"
     tRNA            complement(2915..3004)
                     /locus_tag="OO315_003941"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2968..2970),aa:Ser,seq:cga)
     gene            3098..3895
                     /gene="mtfA"
                     /locus_tag="OO315_003942"
     CDS             3098..3895
                     /gene="mtfA"
                     /locus_tag="OO315_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5892643.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3996..4071
                     /locus_tag="OO315_003943"
     tRNA            3996..4071
                     /locus_tag="OO315_003943"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4029..4031,aa:Asn,seq:gtt)
     gene            4233..5495
                     /locus_tag="OO315_003944"
                     /pseudo
     CDS             4233..5495
                     /locus_tag="OO315_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1509 a         1326 c         1293 g         1464 t
ORIGIN      
        1 ttacctccag atccgttgct ggaatgtgca ggacggacgc ggtggccgtt cggaataagg
       61 gagcctgacg aagattatcc agtgacgata atcgagactg agggctttct taatctcgta
      121 tccgtgtctg cggtagcgct gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg
      181 ctgatacccg tcagatttga atgcatgaga acgccgcccg tgcctgctgg caggggcggc
      241 agagttatcc gaattgtaaa atttggtatc gtgcgccatc tgttttctct gctggcgcag
      301 caggtgccag ttgttcaggc tgacggatgg attgtaaacc agaacgacca gaaaaaacaa
      361 aacccgccga agcgggttaa gtgcgggtgc gttgaggatg cctgacacat cagcggtggc
      421 gagggatttc tcccccgccg ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg
      481 acctccgtct ggccggttcg gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc
      541 gtcactatga cggttaaaca gatgacgata agggcgatta acatcgcctt ttgctgcttc
      601 atagcctgct tctccttgcc tttcggcacg taagaggcta acctacatgt gttcagcatg
      661 gattgagcct cagattaatg ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt
      721 tctctatctg ccttttggtg ttcatgcctg agacagatag cctcaagcac ccgcagtcat
      781 tctacttaac taagatttcc ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta
      841 aaccaatagc gttcgctgta tttaccgcca gtattcaatg cacatgaccg ccatgaacac
      901 ccctaaaaaa agggcattta tatgtccaaa cattaatatc aaaacatcaa ttttttccat
      961 ataccttgct gtgaagatga tgggcataca tgatgcgaac aaccagaacg caacaaacaa
     1021 aaactgcaat gcgtttttca ttattccccc tacaatcaat gtgcaataac atttaaacac
     1081 acctcaattt ggccggacat ataaatatct aaaccagaaa aaatcactta catagcgtta
     1141 caaactcttt agtctaaata ctcatcgtaa aacattcccc atacttatca gtccgttccg
     1201 cgccaggtag cttattgcct tatctggcaa cctataatca ggtttccgct ttttcagttg
     1261 gctggtcgtt taaccgacat agttaaccca ttaatctggt tgccggatgc tggtggattt
     1321 tcgcgtttta attgttcata aaagtgcaca gctttaacca gttcttctga tgtaaccggg
     1381 actggtgggg cagtgaataa ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct
     1441 tttttcggta catatttcca gtcaccaacc cacaactccc cctgagagtc cataacacct
     1501 tttttcacgt agcgatatcg ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt
     1561 ttgaataact cgccctctac tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct
     1621 atttttaatt tggcttcttc gattaattgt tcttttgtta attcagtcat tttcattacc
     1681 gccctggtaa tgctgccacc agcgaaaatg gttaatggaa aacgggcttc cgttctatac
     1741 caatcgtagt ggcaaaccga ttgtcagccg ggatctattt acctgcaata aaactttacc
     1801 accacgcgag gtagagccga attttggtgc gatctgatgg gaagacgaag gaaaaatcct
     1861 gaacacgaaa aattacctcc aaatgtatac ccaaataaat atagttatgt atggaaacca
     1921 acatccagag aatctgtaac actaaccgcc atcaaggatg gtttagctgc tttatggaaa
     1981 aagtatgagg aaactgtaaa taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa
     2041 aaattcctcg ccagcgccta ttacagtgac ctcagtccaa gaacacaaaa agattatctg
     2101 caacatcaaa aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca
     2161 gaacacatcc gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat
     2221 gaaaaaagca gtatgtcccg cgtttacagt tgggggtatg agcgagggta cgtgaaggct
     2281 aacccatgtg caggtgtaag taaattcaag gccaaaaacc gcgaacgata tgtaaccgac
     2341 aaagaatacc aggcagtatt aagcgttgca cctcttcctg tttttatcgc aatggaaatt
     2401 gcctatctgt gtgcagcgag ggtttccgat gtgttatcgc tgaaatggga gcagattgga
     2461 aacgacggga tctttatcca gcaagggaaa acaggaaaaa aacagataaa agcatggagt
     2521 ccacgattac aggcggcgat cgaaaaagca aaacagttac caacatccgc ctatgtaatc
     2581 agcaatcaat acggcaaccg atatatgtac aaaggcttta acgaaatgtg ggtagaagca
     2641 agaaatcgcg caggcaaaat ttcaggtatt ttaaccgact tcacctttca tgatctgaag
     2701 gcgaaaggaa tttcagacta tgaaggaagc agtcgggata agcaactttt ctctggtcac
     2761 aaaaccgagg ggcaagtgct aatctatgac aggaaggtta aagtttcacc gacacttgat
     2821 gtcccgttac ctgaaaatat tccaagaaaa tattccaagt aattccaagt gtgatttttg
     2881 tcactgactt aatgatgtat aagtgattga attttggcgg agagaggggg atttgaaccc
     2941 ccggtggagt tgcccccact ccggttttcg agaccggtcc gttcagccgc tccggcatct
     3001 ctccgttcag atggttgcca tgatgccagg aaatttggca ttttaacagt ccctgtccgt
     3061 gcaattttgt tcaagtgacg agtttgcgag caaaacgatg attaagtggc cctggaaagt
     3121 acaagaatca gcacatcaaa ctgcccttcc ctggcaggaa gcactatcga tccccctttt
     3181 aacgtgtctg acagaacagg aacaaagcaa attggtcgct cttgccgaac gttttttaca
     3241 gcaaaaacgg cttgttcctt tacagggctt tgagctgaat tcattaagaa gctgccggat
     3301 agcacttcta ttttgcctgc ccgttctgga gttaggactg gaatggctgg atggttttca
     3361 tgaagtctta atttatcctg cgccatttgt ggtcgatgat gaatgggaag acgatatcgg
     3421 tctggtgcat aaccaacgta ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt
     3481 tttgaactgg ttggatatac aagattcttt tgatgcttct ggttttaacc tgattattca
     3541 tgaagtcgct cataagctgg acacccgtaa cggcgatcgc gccagcggag ttccctttat
     3601 ttcgttgcgt gaggttgctg gctgggaaca cgatcttcat gctgcaatga acaacattca
     3661 ggaagaaatc gaattagttg gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga
     3721 tcctgctgaa tgttttgccg tactttctga atatttcttt agcgccccag aactttttgc
     3781 tcctcgtttc ccttcattgt ggcaacgttt ctgtcaattt tatcaacaag atcctttgca
     3841 gagactgcat cacgctaatg atacagactc gttttcagcg acgaatgttc attaattaac
     3901 aactttgaag attaattaac caattgaaat ggcttatgaa atttagtgtt gacagacaag
     3961 gtaccgctaa gtaatatgcg ccccgttctc acgattcctc tgtagttcag tcggtagaac
     4021 ggcggactgt taatccgtat gtcactggtt cgagtccagt cagaggagcc aattttctgt
     4081 tttcatgcat ccttgcgaat ctttatctga tgttgattca acaggttagt gtgaaaactc
     4141 ttctcggtgc attttgattt taccctacgc atcgggaaaa attggtggtc aaatctggga
     4201 tcaggttagt tcgataatgg agtgaccccc atatatccct taccgacgca aaaatccgca
     4261 ccctcaagcc ttctgataaa ccctttaaag tctccgattc tcacggtctg tatctgctgg
     4321 tcaagccggg tggctcccgc cactggtatc tcaaataccg tattagcggt aaagaatccc
     4381 gcattgcgct gggtgcctat ccagccatct ccctgtctga tgcgcgacag caacgtgaag
     4441 gtatccgtaa aatgctggcg ctgaatatca acccggtaca gcagcgggct gctgaacgtg
     4501 gctcacgaac accggagaaa gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca
     4561 ggaaatggtc gcagaacacc gccgaccgtc tgcttgccag cctgaacaat cacatctttc
     4621 cggtcatcgg gaacctacct gtatcagaac ttaaaccccg tcatttcatt gacctgctga
     4681 aagggatcga ggaaaaaggt ctgctggagg ttgcgtcccg cacacggcag cacctgagta
     4741 acataatgcg ccatgcggtc catcaggagt taatcgatac gaaccctgca gcaaaccttg
     4801 gcggcgtgac cacacctcct gtcagacggc actatcctgc cctgccgctg gagcggctgc
     4861 ctgaactgct tgaacgtatt ggggcatatc atcagggccg tgaactgacc cggcatgccg
     4921 ttctgctgat gctgcatgtg ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag
     4981 agattgattt cacaaaccga gtctggacga tacccgcgac gcgagaaccc attattggcg
     5041 tgcgttattc cggccgcggg gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac
     5101 agtccatcgc cattctgaaa cagattaagg atatcaccgg taataatgaa ctgatcttcc
     5161 ccggcgacca taacccgtat aagccaatgt gtgaaaacac ggtcaataag gcactgcggg
     5221 tgatgggtta cgacacgaaa aaggatatct gcggtcacgg cttccgggca atggcatgca
     5281 gtgcgctgat ggaatcgggt ttatgggcaa aggacgcagt agaacgccag atgagtcatc
     5341 aggagcgcaa taccgtgcgc atggcttata ttcataaggc agagcacctg gaagcccgca
     5401 aagcgatgat gcagtggtgg tcggattatc tggaagcatg ccgagaatct tatgcaccgc
     5461 cttatacaat tggtaaaaat aagtttatcc catagtatac taacgaatcc cacactctgg
     5521 caaattacca ccacctctac aggaggtggt tctatataga cttttatcaa cttcatgacg
     5581 aaaaacacct at
//
LOCUS       DAJTRD010000174         5557 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTRD010000174 DAJTRD010000000
VERSION     DAJTRD010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5557
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.175"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..641
                     /locus_tag="OO315_003945"
     CDS             <1..641
                     /locus_tag="OO315_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5892644.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCAL
                     GLSRSALVVAAWLLCYGHCKTVDEAIIFIRARRSHIVLKEEHKAMLKLWENR"
     gene            complement(695..1300)
                     /gene="azoR"
                     /locus_tag="OO315_003946"
     CDS             complement(695..1300)
                     /gene="azoR"
                     /locus_tag="OO315_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5892645.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            1501..5403
                     /gene="hrpA"
                     /locus_tag="OO315_003947"
     CDS             1501..5403
                     /gene="hrpA"
                     /locus_tag="OO315_003947"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5892646.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVTIKLFDNPLEQKQAMWNGLRRLLLLNISSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
BASE COUNT         1376 a         1360 c         1539 g         1282 t
ORIGIN      
        1 tcgtaggcgc gtgcattgtg ttgatggagc taatgatgat ggttcagtta tggtggtcag
       61 tctggttatg ttggccagta ttatcgctac tcattattgg ccgtgggtgc ggtgggcttg
      121 gcgcgataac aacagggaaa gatagtcagg ggaaactccc gcccgccgtt tactggctga
      181 cattgccctg gcgcatcggg atgtggctat ctatgcgttg gttttgtcgt cgcctggagc
      241 cggtgagcaa aatgactgct ggtgtttatt taagggcgtt tccacgacat attccggcac
      301 agaatgcggt tctggacgtc acctttgaat tccctcgtgg acgagcgaca aaagatcgac
      361 tctatttctg tgtaccgatg ttggatctgg tggttccgga agagggggag ctccgacagg
      421 ccgtggcgat gctggaaaca ttacgcgaag agcaaggcag cgttctggtc cattgcgcat
      481 tgggattatc gcgcagtgcg ctggtagtgg cggcatggct gttatgttac ggacactgta
      541 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
      601 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      661 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      721 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      781 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      841 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaata acgatggctt
      901 ttttacccgt taccagacct tccggaccgt tctcggtata gcggaaagta acgcctgcgc
      961 gtgcaaccag gtcaaaatag tttttcaact gagttgagat gttgaagtta tacatcggtg
     1021 ccgcaataac gataacgtcg tgggctttca gctcggcaat cagctcatcg gaaagcgcca
     1081 gagcttcctg ctgacgcgga gtcagcggcg catcgctcgg acgcagagcg ccaaccagtt
     1141 cgccatccag taccggaatc ggatttgcag ctaggtcgcg aacggtgatt tcatcagcgg
     1201 agtgcttttc gctccattgt tcaacaaaat aatcggacaa ctgattagac tgagagtacc
     1261 ctgccaggat gctggattta agaactaata ccttgctcat ggtgtttcct tatagatgtt
     1321 tgaatggacg atgccccgtt gcttgctgac actttattca caatcctgcc acagagatcg
     1381 cgcaataaat cgaagtctat gttcgaattt attgaacaac gcatagaaag ccgcgatgtg
     1441 gtactctata tctatcattt aaaagaaaat taatcaggca gactactgcc cactaacgtt
     1501 atgacagaac aacaaaaatt gacctttact gccttgcaac agcggctgga ttcgctgatg
     1561 ctgcgcgaca gactgcgttt ttctcgccgt ctgcattgtg ttaagaaggt taaaaatcct
     1621 gatgcacaac aggccatttt ccaggagatg gcgaaagaga ttgaccaggc ggcagggaaa
     1681 gtcctgctgc gtgaagcggc acgaccggaa atcacttatc ctgacaattt accggttagt
     1741 cagaaaaaac aggacatact cgaagcgatt cgtgatcacc aggtggtgat cgtcgccggg
     1801 gaaaccggct ccggtaaaac gactcagcta ccgaaaatct gtatggagct ggggcgcggt
     1861 attaaagggc tgatcggtca tacccagccg cgtcgcctgg cggcacgaac ggtggccaac
     1921 cgtattgcgg aagagctgaa aacggagccg ggcggttgca tcggttacaa agtgcgtttc
     1981 agcgatcacg taagtgataa cacgatggtc aagctgatga ccgacgggat cctgctggcg
     2041 gagatccagc aagaccgcct gctgatgcag tacgacacca tcattattga cgaagcgcac
     2101 gaacgcagcc tgaatatcga ttttctgctc ggctatttga aagagttgct gccgcggcgt
     2161 cctgacctaa aaatcattat cacttccgcg actatcgacc cggaacgctt ttcgcgccac
     2221 tttaataatg cgccgattat tgaagtctcc ggtcggacct atccggtgga agtgcgctat
     2281 cgcccgattg ttgaagaagc cgatgacacc gagcgcgatc agttgcaggc gatttttgac
     2341 gccgtagacg aactaagcca ggaaagcccc ggcgacattc tgatctttat gagcggcgag
     2401 cgggaaattc gcgataccgc cgatgcgctg aacaagctga acttacgcca taccgaaatc
     2461 ttgccgcttt atgcgcggct ttcgaacagc gaacagaacc gcgtgttcca gtcgcacagc
     2521 ggacggcgca ttgtgctggc gaccaacgtc gcggaaacct cgctgaccgt accggggatt
     2581 aaatacgtca tcgaccccgg tacagcgcgt atcagccgct acagctatcg caccaaagtg
     2641 cagcgtttgc cgattgaacc catttcgcag gcgtctgcca atcagcgtaa aggccgctgt
     2701 ggtcgtgtgt ccgaagggat ctgtattcgt ctctattccg aagacgattt cctctcgcgc
     2761 ccggagttta ccgatccgga gattctgcgt accaacctgg cctcggttat tttgcagatg
     2821 accgcgctgg ggctgggcga tatcgctgcg ttcccgtttg tcgaagcacc ggataaacgc
     2881 aatatccagg atggcgtgcg tctgctcgaa gaactgggcg cgatcaccac ggatgaacag
     2941 gccagcgcct ataaactgac gccgctcggt cgccagctct cgcagttgcc tgtcgaccca
     3001 cgtctggcgc gtatggtgct ggaagcgcaa aaacatggct gcgtgcgtga ggcgatgatt
     3061 atcacgtccg cgctctccat ccaggatccg cgcgaacgtc cgatggacaa acagcaggca
     3121 tcggacgaaa aacatcgtcg cttccacgac aaagaatccg acttcctcgc gtttgtaaat
     3181 ctgtggaatt atcttggcga gcagcaaaag gcgctttctt ccaacgcctt ccgtcgcctg
     3241 tgtcgtaccg attacctcaa ctatctgcgc gtgcgcgaat ggcaggatat ctacacccag
     3301 ttgcgccagg tggtgaaaga acttggcatt ccggtcaaca gcgaaccggc ggagtatcgt
     3361 gaaattcaca tcgccttact gaccggtttg ctttcccata tcggcatgaa agatgccgat
     3421 aaacaggaat ataccggtgc acgtaacgcg cgtttctcca tcttccccgg ttctggttta
     3481 ttcaaaaagc cgccgaaatg ggtaatggtg gcggaactgg tagaaaccag ccgcctgtgg
     3541 gggcgcattg ctgcgcgtat cgacccggaa tgggtagaac ccgttgctca gcatttgatt
     3601 aaacgcacct acagcgaacc gcactgggaa cgggcgcagg gcgcggtgat ggcaacggaa
     3661 aaagtcactg tttatggttt gccgattgtt gctgcgcgca aggtcaacta cagccagatc
     3721 gattcggcgt tatgtcgtga actctttatt cgccacgctc tggtggaagg tgactggcag
     3781 acgcgtcacg cattcttccg tgaaaacctg aaactgcggg ccgaagtgga agagctggaa
     3841 cacaaatcac gtcgccgcga tattctggtt gatgacgaaa cgctgtttga gttctacgac
     3901 cagcgcatca gccacgatgt aatctccgct cgtcacttcg atagctggtg gaaaaaagtc
     3961 agccgcgaaa cgcctgattt gctcaacttt gaaaagagca tgttgatcaa agaaggggcg
     4021 gaaaaaatca gcaagctgga ttacccgaac ttctggcatc agggcaatct caagctgcgt
     4081 ttgagctatc agtttgagcc aggcgcggac gcggacggtg tgaccgtaca tattccgctg
     4141 ccgttactta accaggttga ggaaagcggg tttgaatggc agatccccgg tctgcgccgc
     4201 gaattggtga ttgctctgat taaatcgttg ccgaaaccgg tacgccgtaa ttttgtgcct
     4261 gcaccaaact acgccgaagc gtttttaggc cgcgtcacac cgctggagtt accgttgctc
     4321 gacagccttg agcgcgagtt acggcggatg accggcgtta ccgttgaccg cgaagactgg
     4381 cactgggatc aggtgcccga tcacctgaaa atcaccttcc gcgtggtgga tgacaaaaac
     4441 aagaagctaa aagaagggcg ctcgctacaa gatctgaaag atgcgctgaa aggcaaagtg
     4501 caggaaacgc tgtccgcggt ggcggatgac ggtatcgagc agagcggctt acatatctgg
     4561 agttttggtc agctgccgga aagttacgaa cagaagcgtg gcaactataa agtgaaggcg
     4621 tggccggcgc tggtggatga acgcgacagc gtgacgatca aactgtttga taacccgctg
     4681 gagcaaaaac aggcaatgtg gaatggtctt cgccgtctac tgctgctaaa tatttcctcg
     4741 ccgatcaagt atttacatga gaagttacca aacaaagcca agctggggct gtactttaac
     4801 ccgtatggca aagtgctgga gctgatcgac gactgtatct cctgcggtgt ggatcaactg
     4861 atcgacgcca atggtggccc ggtctggacg gaagaaggct ttgctgcgct gcatgaaaaa
     4921 gtgcgcgccg aactgaatga cacggtggtg gatattgcga agcaggtcga gcaaatcctc
     4981 actgcggtgt tcaatatcaa caaacgtctg aaagggcggg tggatatgac catggcactg
     5041 gggctttctg atattaaagc gcagatgggc gggttggtat atcgcggttt tgtcactggt
     5101 aacggcttca aacggctggg cgacacgctg cgatatttgc aggcgattga aaaacgactg
     5161 gaaaaactgg cggttgatcc acatcgcgat cgtgcacaga tgctgaaagt cgaaaacgtc
     5221 cagcaggcgt ggcagcaatg gatcaacaaa ctgccgcctg cacgtcgtga ggatgaagac
     5281 gtgaaagaga tccgttggat gatagaagag ctgcgcgtta gttacttcgc tcaacaactt
     5341 ggtacgcctt atccgatttc agataagcgt attttgcagg cgatggagca gattagcggt
     5401 taaccctgct atttgcctaa taaatctaaa acccggtaag catttagcgc cgggtttttt
     5461 attaattcta aaacggtaag gacaaaaatt caggaattca gaaaaataca attctcagct
     5521 gcaagatgaa taatgtttat ctacagcatt tccttaa
//
LOCUS       DAJTRD010000175         5485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTRD010000175 DAJTRD010000000
VERSION     DAJTRD010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.176"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..1630)
                     /locus_tag="OO315_003948"
     CDS             complement(<1..1630)
                     /locus_tag="OO315_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5892647.1"
                     /translation="MENSRIPREHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2530)
                     /locus_tag="OO315_003949"
     CDS             complement(1646..2530)
                     /locus_tag="OO315_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5892648.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2541..3146)
                     /locus_tag="OO315_003950"
     CDS             complement(2541..3146)
                     /locus_tag="OO315_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5892649.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="OO315_003951"
     CDS             complement(3317..>5485)
                     /locus_tag="OO315_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR5892650.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1363 c         1141 g         1400 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccta gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DAJTRD010000176         5479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTRD010000176 DAJTRD010000000
VERSION     DAJTRD010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.177"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            426..1334
                     /locus_tag="OO315_003952"
     CDS             426..1334
                     /locus_tag="OO315_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5892651.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1380..3371)
                     /gene="tkt"
                     /locus_tag="OO315_003953"
     CDS             complement(1380..3371)
                     /gene="tkt"
                     /locus_tag="OO315_003953"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5892652.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3649..4407
                     /gene="loiP"
                     /locus_tag="OO315_003954"
                     /gene_synonym="yggG"
     CDS             3649..4407
                     /gene="loiP"
                     /locus_tag="OO315_003954"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5892653.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4613..>5479)
                     /gene="speB"
                     /locus_tag="OO315_003955"
     CDS             complement(4613..>5479)
                     /gene="speB"
                     /locus_tag="OO315_003955"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5892654.1"
                     /translation="FLRLPMNFQPYDSDADWVITGVPFDMATSGRAGGRHGPAAIRQV
                     STNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMSEKLQAHAEKLLAAGKRML
                     SFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCEFDHGTMFYTAPKEGLIDP
                     NHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQIVGDMPVYLTFDIDCLDP
                     AFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVAPAYDQSEITALAAATLAL
                     EMLYIQAAKKGE"
BASE COUNT         1344 a         1439 c         1418 g         1278 t
ORIGIN      
        1 atgtataact gagattgatg attctggaaa ataatcacta agccgcataa attatcctta
       61 ttttattttt agggtaccgt ccggcggtaa aaataaaata ctcgtgcggt ttaatgaaag
      121 caattgggta cgcgagaaga aaaaccccac aaaccaaaat aataaaacct taaataacaa
      181 caatatgaaa ttgatatttt taaagtcaac tttgtatttg ttatgttcat cacaaaatca
      241 acattttgtt tgttattgta tttgtgatgc atatctctat ttatttattg aatgttcgaa
      301 aacatacttt tctaatgcaa tgttttttcg ctatacccct ttatagattc tgaattctca
      361 cctgtcggct gttttctcct catctcatgt gacatattcc catactttac tcaaggaaga
      421 ttgtcatgaa agcacctgtt tcattaacct catttcgccc acaaaaatct cttgctatcg
      481 ccattggcgt gcttgccgtg gttgtgctgc catttctctc gtattacacc gttaatgaag
      541 gtgaacgcgg cattttgctg cgctacggaa aaatcgtaaa agttgccgaa ccggggctgg
      601 gttttaagat cccttttatg gaaagcgtgg agaaaatttc cacgcgtaat caggctgtgg
      661 tctatcaggg attacaggcc tacagccgcg accagcaacc ggcacaaatg acagtatcag
      721 tcagcttcca cattaaacct tcagaagcag gcgcggttta taccacctac aacactattg
      781 aagcattaaa agatcgtcta attgtccgtc agttgccaac tcagctggag aacattttcg
      841 gccaatatac cgccatcagc gccgtacagg atcgtacaaa attggttcag gatctgcaaa
      901 atgccatgcg caaagccgtt gttgggccag tggtaattga cggggtccag attgaaaata
      961 ttgatttttc tgacgcttat gaaaagtcga ttgaaaaccg aatgaaagca gaagtcgcca
     1021 ttgccacgcg taagcaaaat ctggaaacag agaaaatcca ggcgcaaata gcggtaactc
     1081 aggcccaggc agaagcagac agtaaactgg cggcagccaa agcagaagcg gaaactattc
     1141 gcgtaagagg cgcggcagag gctgaaacta ttcgtttaaa aagtgcggct gaagcagaag
     1201 caatccgcct gcgcggtgaa gctctgcggg ataatcccgg acttgtagcc ctcaccactg
     1261 ccgaacgttg ggacggtaaa ctgcccgata ccatgatccc cggcagcaca gttccgttta
     1321 tctctactaa gtaaaaaaag ggccgctttc gcggtccttc tcacaaaacg tcttactgat
     1381 tacagcagcg cttttgcttt cgcaacaacg ttatcaacag tgaagccgaa ctcttcaaac
     1441 agcagctctg ccggagcaga ttcaccgaag gtggtcatgc cgacgatagc gccgttcagg
     1501 ccaacatact tgtaccagta gtcagcaata cccgcttcta ccgcaacgcg tgcagtaacc
     1561 gctttcggca gtacggattc acggtaagcc gcatcctgct tgtcaaatgc gtcggtagac
     1621 ggcatggaca ccacgcgcgc tttcacgcct tcggcagtca gtttttcgta ggcagcaacc
     1681 gccagttcaa cttcagaacc ggtggcgatg aagatcagtt ccggctgacc agcacagtct
     1741 ttcagcacat aaccaccgcg cgcgatgttt gccagctgct cttcagtacg ttcctgctgc
     1801 gccaggttct gacgggagag gataagcgca gtcgggccgt cctgacgctc aacgccgtat
     1861 ttccacgcga ccgcggattc aacctggtca cacggacgcc atgtagacat gttcggggtc
     1921 acgcgcagag aagcgacctg ctctaccggc tggtgagtcg ggccatcttc gcccagaccg
     1981 atggagtcgt gggtgtaaac catcacctgg cgctgtttca tcagcgcagc catacgtacg
     2041 gcgttacgtg cgtattccac gaacatcagg aaggtggagg tgtacggcag gaagccaccg
     2101 tgcagggaga taccgttagc aatcgcggtc ataccgaact cgcgaacacc gtagtggatg
     2161 tagttacctg cagcatcttc gttgattgct ttagaaccag accacagggt caggttagac
     2221 ggtgccaggt cagcagagcc gccgaggaat tcaggcaaca gcgggccgaa cgcttcgata
     2281 gcattctgcg acgctttacg gctggcgatt ttcgccggat tagcctgcag tttagcgata
     2341 aactctttcg ctttggcgtc gaagtcagac ggcatttcgc ctttcatacg gcgggtaaat
     2401 tcagccgctt cctgcggata agctttcgcg taagccgcaa acttctcatt ccatgcagat
     2461 tctttcgcct ggcctgcttc tttcgcatcc cactgagcat agatttcaga cgggatttcg
     2521 aacggtgcat atttccagcc cagctgttcg cgggtcaggg caatttcagc gtcgcccagc
     2581 ggcgcaccgt gggagtcgtg agtaccggct ttgttcgggg aaccgaaacc gatgatggtt
     2641 ttgcacatca gcagggacgg tttgtcagtc actgcgcgcg cttcttcaac tgcgcgtttg
     2701 atggatgccg cgtcatgacc gtcgatgtcg cgaataacgt gccagccgta agcttcgaaa
     2761 cgcattgctg tgtcgtcggt gaaccagcct tcaacgtgac catcgataga aataccgttg
     2821 tcatcgtaga acgcaatcag tttacccagc ttcagcgtac ccgccagaga gcaaacttcg
     2881 tgggagatgc cttccatcat gcagccgtcg cccatgaagg cgtaggtgta gtggtcgaca
     2941 atgtcgtggc ccggacggtt aaactgcgcc gccagcgttt tttctgcaat cgccataccg
     3001 actgcgttgg caataccctg acccagcgga ccggtggtgg tttccacacc agcggtataa
     3061 cctacttccg gatggcccgg agttttagag tgcagctgac ggaagttttt cagttcttcc
     3121 atcggcagat cgtaaccggt gaggtgcagc aggctgtaga tcagcatgga gccgtggccg
     3181 ttggacagca cgaagcggtc acggtcagcc caggacggat tctgcgggtt gtgtttcagg
     3241 aaatcacgcc acaggacttc ggcaatgtca gccataccca taggggcacc cgggtgaccg
     3301 gatttggctt tctgtactgc gtccatgctc agcgcacgaa tagcattggc aagctcttta
     3361 cgtgaggaca ttttgactcc agatcggatg atgaagggca cgcccttaac gacttgacga
     3421 cagcgcgttt tgggctacgc cggaaaattt gccaacaatt taccgcaagc cgcgcgtcat
     3481 gtacatggaa catccttttg ccgcttcaga aatctctgga tcatgctcgc atgttgcgca
     3541 atctgctcgc ccgtccgctg cgcttttcct tatactgaga ctgagcgtcg attcacctgc
     3601 aaacggcgca tttttagaat aatcctgacc ttgtgcggaa gagaaaacat gaaaattcgc
     3661 gccttattgg tagcaatgag cgtggcaacg gtactgaccg gttgccagaa tatggactcc
     3721 aacggactgc tctcatcagg agcggaagct tttcaggctt acagtttgag tgatgcgcag
     3781 gtgaaaaccc tgagcgatca ggcatgtcag gagatggaca gcaaggcgac gattgcgcca
     3841 gccaatagcg aatacgctaa acgtctgaca actattgcca atgcgctagg caacaatatc
     3901 aacggtcagc cggtgaatta caaagtgtat atggcgaagg atgtgaacgc ctttgcaatg
     3961 gctaacggct gtatccgcgt ctatagcggg ctgatggata tgatgacaga taacgaagtc
     4021 gaagcggtga tcggtcacga gatggggcac gtggcgttag gccatgtgaa gaaagggatg
     4081 caggtggcgc ttggtacaaa tgccgtgcga gtagctgcgg cctctgcggg cgggattgtc
     4141 ggcagtttat ctcaatcaca acttggtgat ctgggcgaga aattagtcaa ttcgcaattc
     4201 tcccagcgcc aggaagcaga agccgatgat tattcttacg atcttctgca ccaacgcggc
     4261 atcagcccgg caggtcttgc caccagcttt gaaaaactgg cgaagctgga agaaggtcgt
     4321 caaagctcaa tgtttgacga ccatcctgca tccgccgaac gtgcccagca tattcgcgat
     4381 cgcatcagcg cggatgggat taagtaaagc ctagtggtgt cgaacgtagg tcagataagg
     4441 cgttcacgcc gcatctgaca ttaatggcac atttgttacc ttgtgcgcat tgccggatgc
     4501 gatgctggcg catcttatcc ggcctacggg tgccgaacgt aggtcggata aggcgttcac
     4561 gccgcatccg acattaatgg cacgttttac ccgtgcgcat cgcatctggt gcttactcgc
     4621 cctttttcgc cgcctgaata tacagcatct ccagcgccag cgtcgccgca gccagagcgg
     4681 tgatttccga ctgatcgtat gccggagcca cttccactac gtccatccca acgatgttga
     4741 gatctttcag gccgcgtacc agtttaatgg cgcgatcgga ggtcaggccg ccaatcactg
     4801 gcgtaccggt tcccggcgcg aaagcaggat ccaggcagtc gatatcaaaa gtcaggtaaa
     4861 ccggcatatc acccacaatc tgtttcacct gggcgataac gtcatccacg ctgcgatcgt
     4921 tcacctggca ggcgtccagc acggtaaagc cgttgtcttt atcaaactcg gtacgaatac
     4981 caatctgcac ggaatgattc gggtcgatca gaccttcttt cggcgcggta tagaacatag
     5041 tgccgtggtc aaattcacaa ccgttcgcat aggtatcggt gtgggcgtca aagtgtacca
     5101 gcgccatttt gccgaaatgc ttcgcatgag cacgcagcag cggcagcgta acaaagtggt
     5161 caccaccgaa ggagagcata cgcttaccgg cagccagcag cttctcggcg tgcgcctgca
     5221 gcttttcgct catctcacgg gcatcgccaa aggcatatac cagatcgccg cagtccacga
     5281 cgttcagacg ctcacgcatg tcgaaattcc acgggaagcg gttgtgttcc caggccagat
     5341 tcgtcgaaac ctgacggatc gctgccggac cgtggcgacc acccgcacga ccagaagtgg
     5401 ccatatcgaa cggcacgcca gtaatcaccc agtctgcatc gctgtcatac ggctggaagt
     5461 tcatcggcag gcgtaaaaa
//
LOCUS       DAJTRD010000177         5398 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTRD010000177 DAJTRD010000000
VERSION     DAJTRD010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5398
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.178"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..497
                     /locus_tag="OO315_003956"
     CDS             <1..497
                     /locus_tag="OO315_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
                     /protein_id="HCR5892655.1"
                     /translation="EIAFPANTPVYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHL
                     IANEPGTYDGISASYSGPGFSGMKFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEK
                     LAAPSEYNQVEYFSNVKPDLFADVINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHA
                     ESAH"
     gene            519..2510
                     /gene="cyoB"
                     /locus_tag="OO315_003957"
                     /pseudo
     CDS             519..2510
                     /gene="cyoB"
                     /locus_tag="OO315_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2500..3114
                     /locus_tag="OO315_003958"
     CDS             2500..3114
                     /locus_tag="OO315_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5892656.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3114..3443
                     /locus_tag="OO315_003959"
     CDS             3114..3443
                     /locus_tag="OO315_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5892657.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3455..4345
                     /gene="cyoE"
                     /locus_tag="OO315_003960"
     CDS             3455..4345
                     /gene="cyoE"
                     /locus_tag="OO315_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5892658.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4529..4840
                     /locus_tag="OO315_003961"
     CDS             4529..4840
                     /locus_tag="OO315_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5892659.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4824..4982
                     /locus_tag="OO315_003962"
     CDS             4824..4982
                     /locus_tag="OO315_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005053055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5892660.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAT
                     RIAKEKCA"
     gene            4975..5373
                     /locus_tag="OO315_003963"
     CDS             4975..5373
                     /locus_tag="OO315_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5892661.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1177 a         1344 c         1425 g         1452 t
ORIGIN      
        1 atgaaatcgc tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga
       61 tgaactcctt cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga
      121 ctcgcctgca tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca
      181 gcgggccggg cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat
      241 tcgaccaatg ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt
      301 tcgaaaaact ggccgcgcct agcgaataca accaggtgga atatttctcc aacgtgaaac
      361 cagacttgtt tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga
      421 cccagccaga aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg
      481 cggaatccgc ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg
      541 atgcagtccc gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag
      601 gtctggcgct cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt
      661 ggctgacctc cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga
      721 tgttgctgcg tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg
      781 cgggtgaagc gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg
      841 tgattatgat cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc
      901 cgctgcagat cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt
      961 ttactgttgt tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga
     1021 ccggctggct ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg
     1081 attactggat atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact
     1141 tcttcgttac cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat
     1201 ttacctaggc atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg
     1261 ttaccgtcgc gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata
     1321 tgggtggcaa catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt
     1381 acatcctgat cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta
     1441 aacgtctgtt tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt
     1501 tcatcgtttg gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct
     1561 ttggtatcac cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt
     1621 tcaccatgta tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta
     1681 tcgtcacctt ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact
     1741 tcgttctgca taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg
     1801 tggtcttcgg ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac
     1861 tgaacgaaac ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct
     1921 ttatgccgct gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg
     1981 acccgcagtt ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg
     2041 gtattctctg cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg
     2101 acctgactgg cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc
     2161 cgttctataa ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga
     2221 aagagaaagg cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa
     2281 acagcggtgc aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct
     2341 ggcatatctg gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga
     2401 aaagcttcga cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa
     2461 accagcattt cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg
     2521 cacgcgactg cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc
     2581 ggattttgga tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat
     2641 gccgttctgg tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg
     2701 ttcgttctgg ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct
     2761 atcgccatgt acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg
     2821 tttggtgccg gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc
     2881 atggggccgg atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt
     2941 ctgcacgtca cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc
     3001 ggcctgacca gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg
     3061 gatgtggttt ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc
     3121 attctaccga tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta
     3181 tcctgtcgat cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc
     3241 cggccgtaat tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg
     3301 tgtgcttcct gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct
     3361 tcaccgtgct aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca
     3421 actacaacat gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt
     3481 aacgaaacca ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc
     3541 ctcaaagggc agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt
     3601 tgtggcgtcg ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga
     3661 aaggacgaag aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta
     3721 cgccaccttg ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc
     3781 ctgctggctg ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa
     3841 acgccactca gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat
     3901 tggctactgt gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt
     3961 cagcctgtgg cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca
     4021 ggcggcaaac attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat
     4081 cacgctgtat atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc
     4141 tgggtataaa tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct
     4201 gcgcggttat aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat
     4261 catcgccatc actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca
     4321 tacgctgctg gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct
     4381 ttttattgtc tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga
     4441 tgcacataaa ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga
     4501 tttcattcag ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt
     4561 cgataaaacg ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa
     4621 acttcgtgaa gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc
     4681 tatggaacgc gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag
     4741 ctgggaactg atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc
     4801 gttaatgaaa aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag
     4861 tcctctcctg taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg
     4921 gtaaccctgt tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt
     4981 aatagcgact gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc
     5041 tgtactttta cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca
     5101 caaccttcag aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa
     5161 aagcggggct ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac
     5221 ccggcattac caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat
     5281 gctcgtctcg gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg
     5341 tttttggcga ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggca
//
LOCUS       DAJTRD010000178         5397 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTRD010000178 DAJTRD010000000
VERSION     DAJTRD010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5397
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.179"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            52..978
                     /gene="tauA"
                     /locus_tag="OO315_003964"
     CDS             52..978
                     /gene="tauA"
                     /locus_tag="OO315_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5892662.1"
                     /translation="MAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESGASVDWR
                     KFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEALVVKKTI
                     SKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQRGDIDG
                     AYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAFAKSAID
                     AQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAELTGPVNK
                     AIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            991..1758
                     /gene="tauB"
                     /locus_tag="OO315_003965"
     CDS             991..1758
                     /gene="tauB"
                     /locus_tag="OO315_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5892663.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1755..2582
                     /gene="tauC"
                     /locus_tag="OO315_003966"
     CDS             1755..2582
                     /gene="tauC"
                     /locus_tag="OO315_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5892664.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2579..3430
                     /gene="tauD"
                     /locus_tag="OO315_003967"
     CDS             2579..3430
                     /gene="tauD"
                     /locus_tag="OO315_003967"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5892665.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3536..4510)
                     /gene="hemB"
                     /locus_tag="OO315_003968"
     CDS             complement(3536..4510)
                     /gene="hemB"
                     /locus_tag="OO315_003968"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5892666.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5033..>5397
                     /locus_tag="OO315_003969"
     CDS             5033..>5397
                     /locus_tag="OO315_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5892667.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTT"
BASE COUNT         1258 a         1356 c         1488 g         1295 t
ORIGIN      
        1 cccggttatc ttgaataaat tacctagtca tataacacac ttcttgccgc actggcattc
       61 atcgcttttc aggcacaggc ggtgaacgtc accgtggcgt atcaaacctc agccgaaccg
      121 gcgaaagtgg ctcaggccga caacaccttt gctaaagaga gcggggcgag cgtggactgg
      181 cgtaagtttg acagcggagc cagcatcgtg cgggcgctgg cttctggcga cgtgcaaatc
      241 ggcaacctcg gttccagccc attagcggtt gcagccagcc aacaggtgcc gattgaagtc
      301 ttcttgctgg cgtcaaaact gggtaactcc gaagcgctgg tggtaaagaa aactatcagc
      361 aaacccgaag atttgattgg taaacgcatt gccgtaccgt ttatctccac cacccactac
      421 agcctgctgg cggcgctgaa acactggggg attaaaccag ggctagtgga gattgtgaac
      481 ctgcagccgc ccgcgattat cgccgcatgg cagcggggtg atattgatgg tgcttatgtc
      541 tgggcaccgg cggttaacgc cctggaaaaa gacggcaagg tgctgaccga ttctgaacag
      601 gtcgggcagt ggggtgcgcc aacgctggac gtctgggtgg tgcgcaaaga ttttgccgag
      661 aaacatcctg aggtcgtgaa agcgttcgcc aaaagcgcca tcgatgcaca gcaaccgtac
      721 attgctaacc ctgacgcgtg gctgaaacag ccggaaaaca tcagcaaact ggcgcgttta
      781 agcggcgtgc ctgaaggtga cgttccgggg ctggtgaagg gaaataccta tctgacgccg
      841 cagcaacaaa cggcagaact gaccggaccg gtgaacaaag caatcatcga caccgcgcag
      901 tttttgaaag agcagggcaa ggtcccggct gtagcgaatg attacagcca gtacgttacc
      961 tcgcgcttcg tgcaataaaa ggaggcgcag atgctgcaaa tctctcatct ctatgccgat
     1021 tatggcggca aaccggcact ggaagatatc aacctgacgc tggaaagcgg cgagctactg
     1081 gtggtgctgg ggccgtccgg ctgcggtaaa accaccctgc tgaatctgat tgccggtttt
     1141 gtgccttatc agcatggcag cattcaactg gcgggtaagc gtattgaggg accgggagca
     1201 gagcgtggcg tagtttttca gaatgaaggg ctgctaccgt ggcgcaatgt acaggacaac
     1261 gtggcgttcg gcctgcaatt ggcaggtata gagaaaatgc agcgactgga aatcgcgcac
     1321 cagatgctga aaaaagtggg gctggaaggc gcagaaaaac gctatatctg gcagctttcc
     1381 gatggtcaac gtcagcgggt ggggattgcc cgcgcgctgg cggcgaatcc ccagctgtta
     1441 ttactcgacg aaccgtttgg tgcgctggac gccttcaccc gcgaccagat gcaaactctg
     1501 ctgctgaaac tctggcagga gacgggcaag caggtgctgt tgattaccca cgatatagaa
     1561 gaagcggtgt ttatggcgac tgaactggtt ctgctttcat ccggccctgg ccgtgtgctg
     1621 gagcggctgc ggctcaactt tgctcgccgc tttgttgcgg gagagtcgag ccgcagcatc
     1681 aagtccgatc cacaattcat cgccatgcgc gaatatgttt taagccgcgt atttgagcaa
     1741 cgggaggcgt tctcatgagt gtgctcatta atgaaaaact gcattcgcgg cggctgaaat
     1801 ggcgctggcc gctctcgcgt caggtgacct taagcattgg cacgttagcg gttttactca
     1861 ctgtatggtg gacggtggcg gcgctgcaac tgattagccc gctgtttttg ccgccgccgc
     1921 aacaagtact ggcaaaactg ctcaccattg ccggaccgca gggatttatg gacgccacgc
     1981 tgtggcagca tctggcagcc agcctgacgc gcattgtgct ggcgctactc gcggcggtgg
     2041 tgatcggtat tccggtcggg atcgccatgg ggctaagccc gacggtgcgc ggcattctgg
     2101 atccggtaat cgaactttat cgtccggtgc caccgctggc ttatttgccg ctgatggtga
     2161 tctggtttgg tattggtgaa acctcgaaga tcttactgat ctatttagcg atttttgcgc
     2221 cggtggcgat gtcggcgctg gcaggggtga aaagcgcgca gcaggttcgc attcgtgccg
     2281 cccagtcgct gggtgccagc cgtgcgcagg tgctgtggtt tgtcattttg cccggcgcgc
     2341 tgccggaaat cctcaccgga ttacgcattg gtctgggtgt gggctggtct acgctggtag
     2401 cggcggagct gattgccgcg acacgcggtt taggatttat ggttcagtca gcgggtgaat
     2461 ttctcgcgac tgacgtggta ctggcgggga tcgcggtgat tgcgattatc gcctttcttt
     2521 tagaactggg tctgcgcgcg ttacagcgcc gtctgacgcc ctggcatgga gaagtacaat
     2581 gagtgaacgt ctgagcatta ccccgctggg gccgtatatc ggcgcacaaa tttcgggtgc
     2641 cgacctgacg cgcccgttaa gcgataatca gtttgaacag ctttaccatg cggtgctgcg
     2701 ccatcaggtg gtatttctgc gcgatcaagc tattacgccg cagcagcaac gcgcgctggc
     2761 ccagcgtttt ggcgaactgc atattcaccc tgtttaccct catgccgaag gggttgacga
     2821 gatcatcgtg ctggataccc ataacgataa tccgccggat aacgacaact ggcataccga
     2881 tgtgacattt attgaaacgc cacccgcagg ggcgattctg gcagctaaag agttaccttc
     2941 gaccggcggt gatacgctct ggaccagcgg tattgcggcc tatgaggcgc tctctgttcc
     3001 cttccgccag ctgctgagct ggctgcgtgc ggagcatgat ttccgtaaat cattcccgga
     3061 atacaaatac cgcaaaaccg aggaggaaca tcaacgctgg cgcgaggcgg tcgcgaaaaa
     3121 tcctccgttg ctacatccgg tggtgcgaac gcatccggtg agcggtaaac aggcgctgtt
     3181 tgtgaatgaa ggctttacta cgcgaattgt cgatgtcagc gagaaagaga gcgaagcctt
     3241 gttaggcttt ttattcgccc atatcactaa accggagttt caggtgcgtt ggcgctggca
     3301 accgaatgat attgcgattt gggataaccg cgtgactcag cactatgcca atgccgatta
     3361 cctgccacag cggcggatta tgcatcgggc gacgatcctt ggggataaac cgttttatcg
     3421 ggcggggtga tacgagtgcg gacggttccc tcgccccctt ggggagaggg ttagggtgag
     3481 ggggcgttca ctgtactttc aacaggttaa ctcccccttt ctgagaggaa aaaaattaac
     3541 gcagaatctt cttctcagcc aaatccagcg caaagtagct gaaaatcaga tccgcacccg
     3601 cacgcttaat tgaacctaag ctttcgagca cgactttctc ttcatctata gcacccgcca
     3661 gcgcggcgaa cttaatcatc gcgtactcac cgctcacctg atacgcgcca atcggcaatt
     3721 cagtacgttc acgcagctca cgcacgatgt cgaggtacgc tccagcaggt ttaaccatca
     3781 ggcagtctgc gccctgggct tcatccagca gtgactcacg aatcgcctcg cgacggttca
     3841 ttgggttcat ctgatagctt ttgcggtcgc cttttaatgc gcttcctgca gcttcacgga
     3901 acgggccata aaaggaagag gcgaacttgg tcgaatacga cataatcgcc gtatctttaa
     3961 agcctgcagc gtccagcgcc tgacgaatcg cctgtacctg gccgtccatc gcggcggaag
     4021 gggcgatgaa gtctgcgcct gcagcagctg caaccacggc ttgcttgcct aaattttcca
     4081 gagtcgcgtc gttgtcgacg ccatgctcgc acagcacacc gcagtgaccg tgagaagtgt
     4141 attcacagaa acaagtgtct gacatgacga tcatttctgg cacagtctgc ttgcagatgc
     4201 gcgacatacg cgccaccagt ccatcttccc gccaggcgtc gctgccggtt tcatcggtat
     4261 ggtgagagat gccaaaagtc attacggaac ggataccggc gttggcgatg cgttcaattt
     4321 cgcgtgccag atgtttctct ggaatgcgca tcacgcctgg catggcttca acggctttgt
     4381 agtcgtcaat ttcttcttca acaaagatcg gcaacaccag gtcgttaagg ctaagtgttg
     4441 tctcttcaaa catagcgcgc agcgcaggag atttgcgcag gcgacgaggg cgttggatta
     4501 agtctgtcat ggtctgcctg atgtttgtgg aataaggtgc acagtatacc tgaagcgggg
     4561 caggagtgtt ttacgaaagt tgtcgcgatg ttgacaagaa gagaatgaaa gagagaccag
     4621 gacatctttg taatagaaat agcaataatt tcatattcac tgaaaatatt tttaatcttt
     4681 atttatagct cttggttatt attttttggc gtttggttgt gctgctataa attgaataat
     4741 tcaaactttg ttgtatattg ataagatatt tatctggttt gtatttttga tatttataac
     4801 gcgtatgcat caaaaataac attatctgca acatactgaa atatcgtcct tttatcaatt
     4861 tgatgcgtgt ttttctccat aaattatata tgtccacatt cggacttagg ggaaagaata
     4921 attgaaccat tcgtctgtaa cgcagcataa tcgttagcgc gaaacataat atgttttcta
     4981 tgcattgata attgatggat caacttatta cgtccctgag gagggatgac aaatgcactc
     5041 ctggaaaaag aaacttgtag tatcacaatt agcattggct tgcactctgg ctatcacctc
     5101 tcaggctaat gcagcgacca acgatatttc tggtcaaact tacaatactt tccatcacta
     5161 caacgacgcc acctatgctg atgacgttta ctatgatggt tatgtaggct ggaacaacta
     5221 tgccgctgat agctattaca acggcgatat ctacccggtc attaataacg ctaccgttaa
     5281 cggcgtgatt tctacctact atctggacga cggtatttct accaatacca acgccaatag
     5341 tctgacaatc aaaaacagca ctattcacgg tatgatttat tccgagtgca tgacaac
//
LOCUS       DAJTRD010000179         5388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTRD010000179 DAJTRD010000000
VERSION     DAJTRD010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.180"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            115..2676
                     /gene="mutS"
                     /locus_tag="OO315_003970"
     CDS             115..2676
                     /gene="mutS"
                     /locus_tag="OO315_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5892668.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2782..3434
                     /gene="pphB"
                     /locus_tag="OO315_003971"
                     /pseudo
     CDS             2782..3434
                     /gene="pphB"
                     /locus_tag="OO315_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3485..4252)
                     /locus_tag="OO315_003972"
     CDS             complement(3485..4252)
                     /locus_tag="OO315_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5892669.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4448..5356
                     /locus_tag="OO315_003973"
                     /pseudo
     CDS             4448..5356
                     /locus_tag="OO315_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
BASE COUNT         1319 a         1388 c         1404 g         1277 t
ORIGIN      
        1 gttcgcttct tcccctgaaa tgattaactc cggtatcatg tgcgccttat gtgattacaa
       61 cgaaaataaa aaccatcaca ccccatttaa tatcagggaa ccggacataa ccccatgagt
      121 gcaatagaaa atttcgacgc ccatacgccc atgatgcagc agtatctcaa gctgaaagcc
      181 cagcatcccg agatcctgct gttttaccgg atgggtgatt tttatgaact gttttatgac
      241 gacgcaaaac gcgcgtcgca actgctggat atttcactga ccaaacgcgg tgcttcggcg
      301 ggagagccga tcccgatggc ggggattccc taccatgcgg tggaaaacta tctcgccaaa
      361 ctggtgaatc agggagagtc cgttgccatc tgcgaacaaa ttggcgatcc ggcgaccagc
      421 aaaggtccgg ttgagcgcaa agttgtgcgt atcgttacgc caggcaccat cagcgatgaa
      481 gccctgttac aggagcgtca ggacaacctg ctggcggcta tctggcagga cagcaaaggt
      541 ttcggctacg cgacgctgga tatcagctcc gggcgttttc gcctgagcga accggccgac
      601 cgcgaaacga tggcggcaga gctgcaacgc tctaatccgg cggaactgct gtatgcagaa
      661 gatttcgccg agatgtcgct gattgaaggc cgtcgcggcc tgcgccgtcg cccgctgtgg
      721 gagtttgaaa tcgacaccgc gcgccagcag ttgaatctgc aatttggcac ccgcgatctg
      781 gtcggttttg gcgtcgagaa cgcgccgcgc ggactttgtg ctgccggttg tctgttgcag
      841 tatgcgaaag atacccaacg cacgaccctg ccgcatattc gttctatcac catggaacgt
      901 gagcaggaca gcatcattat ggatgccgcg acacgtcgta acctggaaat tactcagaac
      961 ctggcgggcg gtgcggaaaa tacgctggct tctgtgctcg actgcaccgt cacgccgatg
     1021 ggtagtcgta tgctgaaacg ctggctgcat atgccagtgc gcgatacccg cgtgttgctt
     1081 gagcgccagc aaactattgg cgcattgcag gatttcaccg ccgagttgca gccggtactg
     1141 cgtcaggtcg gcgacctgga acgtattctg gcacgtctgg ctttacgaac tgctcgccca
     1201 cgcgatctgg cccgtatgcg ccacgctttc cagcaactgc cggagctgcg tgcgcagtta
     1261 gaaactgtcg atagtgcacc ggtacaggcg ctacgtgaga agatgggcga gtttgccgag
     1321 ctgcgcgatc tgctggagcg agcaatcatc gacacaccgc cggtgctggt acgcgacggt
     1381 ggtgttatcg catcgggcta taacgaagag ctggatgagt ggcgcgcgct ggctgacggc
     1441 gcgaccgatt atctggagcg tctggaagtc cgcgagcgtg aacgtaccgg cctggacacg
     1501 ctaaaagttg gctttaatgc ggtgcacggc tactacattc aaatcagccg tgggcaaagc
     1561 catctggcac ccatcaacta catgcgtcgc cagacgctga aaaacgccga gcgctacatc
     1621 attccagagc taaaagagta cgaagataaa gttctcacct caaaaggcaa agcactggca
     1681 ctggaaaaac agctttatga agagctgttc gacctgctgt tgccgcatct ggaagcgttg
     1741 caacagagcg cgagcgcgct ggcggaactc gacgtgctgg ttaacctggc ggaacgggcc
     1801 tataccctga actacacctg cccgaccttc attgataaac cgggcattcg cattaccgaa
     1861 ggtcgccatc cggtagttga acaagtactg aatgagccat ttatcgctaa cccgctgaat
     1921 ctgtcgccgc agcgccgtat gttgattatt accggcccga acatgggcgg taaaagtacc
     1981 tatatgcgcc agaccgcact gattgcgctg atggcctaca tcggcagcta tgtaccggca
     2041 caaaaagtcg agattggacc tatcgatcgc atctttaccc gcgtaggcgc ggcagatgac
     2101 ctggcttccg ggcgctcaac ctttatggtg gagatgactg aaaccgccaa tattttacat
     2161 aacgccaccg aatacagtct ggtgttaatg gatgagatcg ggcgtggaac gtccacctac
     2221 gatggtctgt cgctggcgtg ggcgtgcgca gaaaatctgg cgaataagat taaggcattg
     2281 acgctgtttg ccacccacta tttcgagctg acccagctac cggagaaaat ggaaggcgtc
     2341 gccaacgtac atctcgatgc gctggagcac ggcgacacta tcgcctttat gcacagcgtg
     2401 caggacggcg cggcgagcaa aagctacggt ctggcggttg ctgctctggc gggcgtaccg
     2461 aaagaggtta ttaagcgcgc acggcaaaaa ctgcgtgagc tggaaagcat ttcgccgaac
     2521 gccgccgcga cgcaagtgga tggtacgcag atgtctttgc tgtcagtgcc agaagaaact
     2581 tcgcctgcgg tcgaagcact ggaaaatctc gacccggatt cactgactcc gcgtcaggcg
     2641 ctggagtgga tttatcgctt gaagagtctg gtgtaataac aattcccgat agtcttttgc
     2701 tatcgggaat attaacgaca actgacgaat caaataaaaa taccctgtat aataggaaag
     2761 cttattttac agggtaaaac catgccatct acacgctacc aaaaaatcaa tgcccatcac
     2821 tatcgccata tatgggtcgt tggtgatatt catggtgaat atcagttatt acaatcccgc
     2881 ttacatcaac tctctttttt ccccgaaacc gacttactta tttctgtcgg cgataatatt
     2941 gatcgtgggc cggagagtct tgacgtcctg cgcctgctaa accagccctg gtttatctcc
     3001 gttaaaggca accacgaagc aatggcgctg gatgcgttcg cgaccggcga tggcaatatg
     3061 tggcttgcca gcggtggtga ctggtttttc gatttaaatg attgcaacaa gaggcaatag
     3121 ttctgttgct gaaattccat caccttccac atattattga aatcactaac gacataataa
     3181 aatatgtcat cgcacatgca gattatccgg gtgatgaata tctctttggc aaagaaatag
     3241 cggagagcga attactctgg cctgtttatc gtgtgcagaa atcgcttaat ggcgagttac
     3301 aacaaataaa cggcgctgat tattttatat ttggacatat gatgtttgat aacattcaga
     3361 cgttcgctaa ccagatttat attgataccg gatcgccgaa aagtgggcgg ctgtcatttt
     3421 ataaaataag gtagtctcat gcttcttctg tgaagcatga gtaaccagat gttatttcag
     3481 gccattattc atttttcgct accagcaaag agagatcctg cttcgccagc gcgcgactgg
     3541 cactctccgg caaaccgtcg tctgtaataa tctgatcaaa ctcgcttaat ggtaacgcca
     3601 gccatgtcgc cacctgacca tatttcgtcg catcacagac caaaactcgc tggcggctgg
     3661 cactggcaat cgcccgtttc accgtgactt tatcttccgc tggcgtagaa atcccccgca
     3721 cactccatga cgatgcagaa ataaaagcct gatcaatcat caggctgcac agcatggtcg
     3781 cagcggcttc cccgacacag gaacggtttt cccgacacac tgcaccgcca gtgtgaataa
     3841 ttgtgcaatt actgttgtcg agcaagtagt tcgcaataac gaaatcgttt gtgaccacag
     3901 tcagtgactc catgtgaatc agatgctgtg ctatcgctaa cgtggtcgtt cccgcatcca
     3961 gatagataca acttcccggc agaacaagac ttgccgccag cttgccaata gccgcttttt
     4021 gcgtcattgc cagcgcagtt tttacctgat gagaaggttc atgcgccacg cgtcccggag
     4081 actggacgcc tccggacacc agcacaacgg ctccctgctg ctccagtttt tgtaaatccc
     4141 gacgaatggt catatgtgac acattaattc tgtccgttag ttcagcaata ctgacaatgc
     4201 ctttttcagc taccatctca aggatgattt ggcgacgctc tacgggtatc aacttttgct
     4261 ccttcctttg tcctgctgac attctacgct atttgcctgc gaaacgtgcg cggcgcaact
     4321 aacgcttagt tcacataaaa taacacaaaa tgttaattta tgtgaatcat ctcaccatat
     4381 cgttatctcc cagcgcttat gttcacaata tcaaacaaaa tatcacttaa attaacaagg
     4441 agagcagatg aaaacgggat ctgagtttca tgtcggtatc gttggcttag ggtcaatggg
     4501 aatgggagca gcactgtcat gtgtccgcgc aggtttttct acctggggcg cagacctgaa
     4561 cagcaatgcc tgcgctacgt tgaaagaggc aggtgcttgc ggggtttctg ataacgccgc
     4621 gacgtttgcc gaaaaactgg acgctctgct ggtgctggtg gtcaatgcgg cctaggttaa
     4681 acaggtgctg tttggtgaaa caggcgttgc acaacatctg aaacccggta cggcagtaat
     4741 ggtttcttcc actatcgcta gtgctgatgc gcaagaaatt gctaccgctc tggctggatt
     4801 cgatctggaa atgctggatg cgccagtttc tggtggtgca gtaaaagccg ctaacggtga
     4861 aatgactgtc atggcctccg gtagcgatat tgcctttgaa cgactggtac ccgtgctgga
     4921 agccgttgcc ggaaaagttt atcgcatagg tgcagaaccg ggactaggtt cgaccgtaaa
     4981 aattattcac cagttgttag cgggcgtaca tattgctgcc ggagccgaag cgatggcact
     5041 tgcagcccgt gcggggatcc cgctggatgt gatgtatgac gtcgtgacca atgccgccgg
     5101 aaattcctgg atgttcgaaa accggatgcg tcatgtggtg gatggcgatt acaccccgca
     5161 ttcagccgtc gatatttttg ttaaggatct tggtctggtt gccgatacag ccaaagccct
     5221 gcacttcccg ctgccattgg cctcaacagc attgaatatg ttcaccagcg ccagtaacgc
     5281 gggttacggg aaagaagacg atagcgcagt tatcaagatt ttctctggca tcactctacc
     5341 gggagcgaaa tcatgatcaa gattggcgtt atcgccgatg attttacc
//
LOCUS       DAJTRD010000180         5340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTRD010000180 DAJTRD010000000
VERSION     DAJTRD010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.181"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(75..557)
                     /locus_tag="OO315_003974"
     CDS             complement(75..557)
                     /locus_tag="OO315_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892670.1"
                     /translation="MIKPFLMTVAILTSCCAQAKILTNEEIVSRFISNVKTSADNDVD
                     LDTDGFRQLRISCPSPSASGAIILRKASYEFGKSIGVFDFANNASPATMTFLIPIYKD
                     DDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAGVNVNGESGVTYENCKLNMKDINDD
                     "
     gene            complement(625..2574)
                     /locus_tag="OO315_003975"
     CDS             complement(625..2574)
                     /locus_tag="OO315_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5892671.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLSLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKEHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2659..2994)
                     /locus_tag="OO315_003976"
     CDS             complement(2659..2994)
                     /locus_tag="OO315_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5892672.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPCSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2994..3350)
                     /locus_tag="OO315_003977"
     CDS             complement(2994..3350)
                     /locus_tag="OO315_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5892673.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(3350..4846)
                     /locus_tag="OO315_003978"
     CDS             complement(3350..4846)
                     /locus_tag="OO315_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HCR5892674.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHVYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(4843..5004)
                     /locus_tag="OO315_003979"
     CDS             complement(4843..5004)
                     /locus_tag="OO315_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5892675.1"
                     /translation="MFVKPLKGRSVPDPARGDLLPSEGRNVEESSYWFRRIAAGDVVR
                     VKQDKAKKS"
     gene            complement(5014..>5340)
                     /locus_tag="OO315_003980"
     CDS             complement(5014..>5340)
                     /locus_tag="OO315_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892676.1"
                     /translation="KGQWAAYDAVHDVRRELWKALLGWMPDPQGGEIVYVGGTLLDLN
                     RYELYYQFDFTAKYEITEEDTRQAEDVNALPDLSLLSIDVDYIDPGTGPDGDIEHHLE
                     MRFPQN"
BASE COUNT         1274 a         1557 c         1149 g         1360 t
ORIGIN      
        1 aaagagaagg taaagaaggt aagtccatac tgtctccact ataaaaaaac cgccaatttc
       61 tggcggctta tatattaatc atcattaata tctttcatat tcaatttgca attttcgtag
      121 gtcactcccg actcgccatt aacattaact ccagctctga tattcccgtt ttcttttaca
      181 tctacaaaaa actggcctct tggcatcttg aacgcaaatg taaaaccaac agtatctgaa
      241 tcaaaatcat catctttata tattgggatc aaaaatgtca tcgtcgctgg tgatgcattg
      301 tttgcaaaat caaatacacc tatacttttg ccgaattcat aagaagcttt tctcaatata
      361 atcgcaccgc tggcagaagg ggatgggcac gatatcctca gttgtctgaa tccatctgta
      421 tccaagtcaa cgtcattatc tgcagatgtt ttaacgttac taataaatcg tgaaacaatc
      481 tcctcatttg ttagtatttt cgcctgagca cagcaagagg ttaatattgc aaccgtcatt
      541 aaaaatggct ttatcacaaa acaaactcca ttgttagcat gttacgcgat aattttgcct
      601 catctttcac cacaaaaaaa gaactcacct tttaaatgga gaatacccaa catcatgggt
      661 gattttcata aagggatcgc ctgttttcgg caaatcgata acgcgcattc caggcggcgc
      721 gttgtcaaac gtcaccttaa gttcgctgcg ggttgccggt gaagatgcaa ggcttaacag
      781 ctgattcctt tccagtgata cattcggggt gtagcgttta tcgggagata aatacccatc
      841 accacgtggc atctgccatc cggtcatgtc atagacaaaa tcatggaact gtttgcccca
      901 ctcgtcgaac ttatcattca gaccaaatcc actgttcagt gcatcagcca caaaattttt
      961 aacaagccag ggatgttctt tttcaaactc ctgcgccatc atgcccagtt caagcaatcc
     1021 accaatcaga ctgatctttc cgaggccctt taatttcagc cctgtttttc ctgccgccgt
     1081 tgtccattga ttctgggcga ctgttgccgc acgaatactt gccaccgcct tcacaccaat
     1141 atacagggca gaaaccgttg ctattgtttt tactgcctgt tcccatcccc ccattgctct
     1201 gacaacctga tcaacctcct gccagacttt tttcacaacc ggaccgaccg tttcccagtt
     1261 atcaattatg agatatgcac caccgacgag aagcgcaatt aatccctttg ccggcgtcat
     1321 attcatcacg ctacccatga tttttgttat gcgtgtcaat gtgccaatcg ccacgcccat
     1381 cgtcagtaat gccgcaccag ttttcgcgat ggtttttacg acctcaggat tttctctgac
     1441 aaacgtgcgc acttcttcca gaaaaggctt catctctttt atgccttcat tgagtgaagg
     1501 caggaaggtt tcccccagcg tggaagaaat cgcattgatc tggttctgca gcagcaacaa
     1561 ctgattttct gtcgtcgcgg cacgggcggc atattctttc tgcattgagc cgccatactg
     1621 ctgcgcatcc gccacacgat taaagttggt tcgcaacaaa tccagattcg tgagcagcgg
     1681 cgcaatggcc cccagggatt ccttcccgaa cagcgctttc agcacagcag cctgtttttc
     1741 tttcggcact tttgccagag aatccagtac gtgcagcatg gccccgcggg cgtctttctg
     1801 catatccgcc gccagttttt tcgggctaat gcgcaacgag cggagcactt ttttctgcga
     1861 tttggtggca gaatccctcg ctgtcagcga caacataaaa ttctttatcc ccgtcgcagc
     1921 aatttctgac tccaccccca ttccggcaat ggttgccccc attgcggcaa tctctccgga
     1981 ggccacaccc gcaacactgc ctaaagggcc aatacgggtc acaacatcag aaatcttttt
     2041 cgcacttgca ggaccggtat tgccaagata gttaatctta tccgcaagcc ctgccacctc
     2101 tccctgtgtc agtttaaagg cagtgcgcca ctgcgccatc atctgaccgg attcttctgc
     2161 cgtggtgtca aaggccacgc ccatcttcac ggcatcgtca gtaaactgca tcagctcgtc
     2221 acgggcgata ccagcctgac cacccgccgc cacaatttcg gcgatacctt ccgccgacat
     2281 gggcagttct gttgacaggt cgcgcacctg ctccgtcatt gccttaaacg cttccggcgt
     2341 atccagacca tccaccactt tccggacatc cgccattttt gattcaaggg caatggcaga
     2401 tttgaccggg agcgccagcg cccccagtac tgcggttccg gcacccgctg cgcccagtga
     2461 caggctggca aactcctttt taaaaccttt cagctggcgc tgcattccct tcagcggtgc
     2521 cgatacctta tccacggcag tgatgatcgc cttcagctga aaactgtcag ccatgcttca
     2581 tctcctcgtt aatgcggacg gcctcggctt ccagttctgt aaactgcgaa acagccaccc
     2641 gtcgcagctc cagtggattc agtttccaga accaggcaac attgtagagt cgtttccgga
     2701 ggtgcttccc gtctccgact gggtaaaaaa acgcaggatc tgcatgctgg tcttaaaaat
     2761 atccagtttt gccatctgcg ccgccgatga acacgggatc cccgccagca acgggatata
     2821 tttcagtgcc acctggctgt ccagtttaat actgccttca ccggaaataa tgaaaggaaa
     2881 acccagcgcc tcgatttcgt catacgtggg ctcgcgcaac tccagcacat gcagcatttc
     2941 gttatgcgct gtcaccggtt tttttaaaac tatttctgta acgttcattg ccattactga
     3001 taccctccct cttcgccgtg gaattcaaga tctgccgtgc cttcttcggc attatgatta
     3061 gcctctccgt gcagccatgc cgccgaaagc acatacacca tgccgtttgc cagctcagcg
     3121 gtaatcgtca tctggtctga ggtggtaatt ttgtcgaccg gaaagttttt gggcacttta
     3181 aacgtgccct taacgtaagg tgaccgccag gtctccttgt aatccacatc ccctgccatg
     3241 ccgacgatgt catcccggac attggtgttc atcggcactt caatgccacc tgtcagcgat
     3301 aactgctgac catccacttt aaaaaaacag gttccggcga tctttgccat tatgctgact
     3361 cctctgaata ctgaagacgg aactggttaa ccaccgcaaa gacacgtaac tgattaacat
     3421 aatccggcgg gaacagcgta ttcagtcggt tcgggttatc tgcatcacgc tcaactatca
     3481 gatactgttt aaacagatcg taattttcca caataccggc acgctccatc tgacgatatg
     3541 tcgccagaag ttcccctttg ataacggcag gagtgacaat cgcctgcccc ggaccaaaac
     3601 gggtaccatc atttgccagc ttgtgacgtc cgtacttgct tgtgatgacc gatttcagtt
     3661 tgcgaagaac atatgcactg gtatgcagag tttcactgtc cagatagctg ttgtctgcca
     3721 cgccatacgc attctttttg taggtggtta cggaacgctg gatctgcaac gtgccgcctt
     3781 ccacataagc cgtcgccacg ccgtgagata aaagggtctg ctgctctgtc atgatgaatc
     3841 gcttaccttt cggtgccgga agcatcccca ccagctcccc cgtctgtgtc ggacgggcag
     3901 gatcattccg gataaatacc gcttcacggg caaggcgact ggcaaccagt tcatcgacag
     3961 gcgactgggt ttctttttcg taaccggcaa gcgtgatatg ttgctgatta tgcatatctc
     4021 cggcaccaac cagctctgac agcgttccca gttttgcggt atagacatgc ccgtataact
     4081 ggcgcgcata actccagcga ccgctgctgt cattcatttc ggtcatcatc atattgatgg
     4141 aggcggcatc gttgaacggc agaccgataa agtcgaatgc ctcatcgccc atagcagcaa
     4201 cagcggcggt aagatcaggc gctccgctac cggcagcacc gacttccgtc accacccgaa
     4261 gccctgctgg caaaatctca ccaccaccag aaccataata attcagacag accggtaact
     4321 cattaccata cagcccctta tgacgggcag tcagcgtcac cacgcctgca tcagatgacg
     4381 ccgtaaacgg cagggtgata accccgttta ccgcttcctt aatcgcggtg gcaaccgcag
     4441 tggcatcatc gccattcacc acaggcacct gtacacggga gcgcccgaca tacagactca
     4501 gggtgccgct ttcctctgct tctccggcaa ccgtcaccct gaccgtcgcc gccgcccctc
     4561 tggcttccgg tactgcaata acatacagtt caccgaaagg atctgtctga cggtagactt
     4621 ccaccatacg cgccagctgg ctccccgccc cacaaatctg gcgggcataa tctgccgatg
     4681 gcatcagcac caggctgtta acctcaatgg ctgcatcgtt gctggcatgc ccgatcagta
     4741 atgcaggcgc gctggtcacc gctgtatttg ccgcagaatt gttcatctcg gcgtagaaca
     4801 acggcaccag cgtattcgac ggaacagtat taaaacttat cgtcatgatt ttttagcctt
     4861 atcctgttta acacgtacca catcacccgc cgctatacgg cggaaccagt agctgctttc
     4921 ttccacattt cgcccttcgg aaggcaaaag gtctccacgg gcagggtcag gaaccgaccg
     4981 ccctttcagg ggtttcacaa acatgaggga ctcttaattc tgagggaaac gcatttccag
     5041 atggtgctca atgtcaccat ccgggccagt accaggatcg atgtaatcca catcaatact
     5101 cagcagggat aaatccggca gggcattcac gtcctctgcc tgtcgcgtgt cttcttccgt
     5161 gatttcatac ttcgccgtaa aatcaaactg gtaatacagt tcgtaacggt tcagatccag
     5221 cagggtgcca ccgacataaa caatctcgcc cccttgcggg tccggcatcc atccaagcag
     5281 ggctttccag agttctctcc ggacatcatg aacggcgtca taggctgccc actgcccttt
//
LOCUS       DAJTRD010000181         5338 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTRD010000181 DAJTRD010000000
VERSION     DAJTRD010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.182"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO315_003981"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="OO315_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5892677.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKGRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="OO315_003982"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="OO315_003982"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5892678.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="OO315_003983"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="OO315_003983"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5892679.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="OO315_003984"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="OO315_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5892680.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1310 a         1290 c         1458 g         1280 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agcggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg
      181 cggttaacga cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca
      241 tatttcgcca gacgaccttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc
     1201 gcgcgaccta gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattac tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcaacag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaatattc tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcgggg
//
LOCUS       DAJTRD010000182         5261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTRD010000182 DAJTRD010000000
VERSION     DAJTRD010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.183"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            557..1348
                     /locus_tag="OO315_003985"
     CDS             557..1348
                     /locus_tag="OO315_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892681.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1734..1940
                     /locus_tag="OO315_003986"
     CDS             1734..1940
                     /locus_tag="OO315_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5892682.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            2026..2598
                     /locus_tag="OO315_003987"
     CDS             2026..2598
                     /locus_tag="OO315_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892683.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2600..2884)
                     /locus_tag="OO315_003988"
     CDS             complement(2600..2884)
                     /locus_tag="OO315_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892684.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2855..2998
                     /locus_tag="OO315_003989"
                     /pseudo
     CDS             2855..2998
                     /locus_tag="OO315_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4167..5186
                     /locus_tag="OO315_003990"
     CDS             4167..5186
                     /locus_tag="OO315_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5892685.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
BASE COUNT         1693 a         1158 c          993 g         1417 t
ORIGIN      
        1 ataacagaca ttacttaaca aagaacatta cagtcagtgt gtacattaca gatttatatt
       61 caggtgttgc gggactacat tacagcagag aggggtaagg acagagttac aagtactact
      121 agagagaata tcactgcaca gatacttcta tgagtatatc tttatgagag atccgtctac
      181 aggattgggt tataaggtgt atttacaagg tataatcaca ggatcattta catgaccata
      241 cttctgtaaa taatactaat taaccttaat tattttttta tggtacatga actacctgta
      301 gctgtaacga taaacaaatc acccaaaatg aaaaatactg gttatcacag ttcagagaac
      361 tcgtatgagt gtccccctga gcaatgcttc attttctgtg attctgtgtg atagagttat
      421 actgagtaca gtggcagtaa cactgctttc catcacttga ggataaaatt cattaaaaaa
      481 ttaattgcat gaattaacag gtttttttat gaaatgttat gcatctcaac caatatgatt
      541 aacagaaaac ttcatcatga caacacgtat aacgttcagt atgaccagtg atgagacgct
      601 gcgaattgtg gatgagtact gtcataccca caaactttcg cgctctaagg taatcaacgc
      661 attactcagc gccacagcac ctgtccttaa tgatattaac tgttattatc aacttgcagg
      721 cgaactgcag tcacgcttat taaatggagt gtatcaacgg gatcttcctc gcaaacgcaa
      781 tgtcgtcagt gcagagaagt attgtctgga aatctgggaa aacaaacttt tcaccagaag
      841 aatcctggag tttgacagtt caaatggtgt tctgtacgcg ctgaaacata aaagacacta
      901 tcgccgggat aagatgattg gaagggttga aagtcggtgt atcaaagaca tttgcgaata
      961 ccagatgcag ctatccgggg agaagacgaa atatgcctgt tttatttata tcgaaagaac
     1021 aatatacaat cacgataatc catctgataa aatacctgta aaagcggctg ttggtaatgc
     1081 agtcatcctg ctggcaaagg atgtgatata taacgagtac ttttttgatt tgcggcagtc
     1141 attttttgtg agcgttacag atctgatggt ctctggcgca aaaggtattc cggagacaca
     1201 gacatatccg gacgtttatt gctggatccc tcttttttcc ataaattccg gcgtgctcat
     1261 tacccctgta tataaaattg accctctgaa acccgtaacg gtaaaaaaac cggataagat
     1321 cactgttgtt tgtaattaca gagaataatt tcagccttct accttcaccg cgacagtgtt
     1381 tacctctgct ttatcttccg ttcagtaaat acaaaccacc gctctggcag gtacagggcg
     1441 tggtttatct ccgatactcc cctgtgcagt aataatcatc actgaaaata ctgatcccgt
     1501 cacggcaaaa cgccatccca tattccggca acacaaaatc atcttccgtt tataccggat
     1561 tcactgacaa aacagacaac ctgtattgcc tgtacacaac agtttctctc ctggcaggaa
     1621 tattccccta ctcatttttt acgtttctcc ccggttgtta tgccggtgaa ggtggtgcgt
     1681 cgttttcatc accaacgccg gttgagatta acaacatccg gaggaacatt ctcatgaata
     1741 ccccagtttc actgatggat gaccagatgg tcgacatgtc gtttatcact cagctgaccg
     1801 gcctgaccga taagtggttt tataaactca tcaagcatgg agcctttccg ccccccatca
     1861 aactgggccg tagctcccgc tggctgaaaa gcgaagtgga agcctggctg caggcgcgta
     1921 ttgcacagtc ccgtccgtaa ttcctgaccg ttatccgttc caccgcagca aaggcatgat
     1981 ccggcccccg gaggcatttt gctgcgcgct aaaggagaga atattatgca acaacttgaa
     2041 aacaggtgtg acctgctgct catccagcat cagaaatgga tgacaagcgt gacgaggctt
     2101 atcgtcgctc atggaatggg gtctccgcat cttcatggtt atcaccgtct gaccctggca
     2161 cacttttttc tgccggaaaa aggctccgtc atttcggtgg ccccccaggg actctatcag
     2221 gtcgtcaacc cgggaacacc accgttcatt cctgccattc aggaagggtt aatgaccagc
     2281 attcagaccc atgaaatcat gctgcttaca cattttaatc tgggcggcgt tttactcagt
     2341 gagctacatc gtctgggtga aaaccggctg gcaaacaggc tgaacagcct gctccggcgt
     2401 ttcgacgatc gtgatctcta ccacacgctg atatggctgt gctggtatga tctgatgtgt
     2461 gcccactcca tgcagccctg gactgaagag ttaaagcata aatcacatgc agagcttgaa
     2521 aactgggctg tggcgcgcaa acgggaaaag cgcgagctgg aactgatgat tgacgagtac
     2581 ctgttatacg cctgctgatt cataacgttc ccggggttcc ttcctcatcg ttacacacag
     2641 ccggacggct atctatggct gtccgccaaa tgaccatcgc cgcatttcgt tccgctgaaa
     2701 acggtacgta gtcagtgata ccggaatttc cggagagggc cgcaggtata ttgcttctgt
     2761 gcaattgatg cttatttaca ttggatgtag ttatttctgc atccaatgct atcggaaaga
     2821 gattaaccag caaatggttc ctcaaatcgt attgatattt acccggcgta ttgagcacca
     2881 ccatctgaca ctccgttccc gcatcaaacg actggaccga aaaacgattt acttttcacg
     2941 tgctgtggaa attcacgaaa aaattattgg gtcattcatc gaaaaacata tgttctgatg
     3001 actggagtca tcctccagaa atatacgtca actgaacaat atcgattttt tatgggattc
     3061 tatacacttt tccatatccc tctgaaaatc agtcatcagc tcaccaacac ctcttctgaa
     3121 acttgtatta aacttattgc ttaaatagat gatataacat ctcttccatt gcactgaatc
     3181 ggcgaaaact gccgtttcaa taatctcaac aatattattt atcggctaac tttatcagta
     3241 aacaatggaa tgtcacactg atgaaatgaa cattcagagt aagatgataa aattcatata
     3301 aacgccattg ctgaatatta tttatttcat acagataaac gataaaatac aaaagggtat
     3361 atttctcaat gtaacagtca attattcatc tccactggcg agcacggtca gcacacaaac
     3421 aaagttagtc ctcctgctta atcattgcgt atcagaaact aaaaaagaaa aaattctgca
     3481 gacaacttaa caatcttcat cacatttcct gaagacagac atatccacaa acggtagtca
     3541 gcgttgaatt aacaagtctt acagagctat acgtagctta taaagccact caatgttcat
     3601 catcacccga ctacttatct ggaataccac cagtataaac tcatcaataa aaacacctgt
     3661 tgttaaaata agaatactgt cagcagtaaa ttattaaaac aacatctgtc cacactatct
     3721 gttcttttaa cgccaacggc agcgaaaata gtgcatcaca gccttctttt ttctttcgcc
     3781 accactcaga caaacacaac actcacaccc acaccacaaa acagaatcaa aacataaaat
     3841 taacaataac agtcaaaagg aaaacacaga atattgaggt ggactgtcac caggacagcg
     3901 catacgacgc atgtgatcag cacatcccgt tcttcgctgg catccccaaa atgcagaaca
     3961 aacaatcata acattatgaa tataaaagaa aatatgattt ttcaccatga aaaaaacatc
     4021 aatgtatctc tgtataaaaa taatgataac atgacataaa catttaaaaa cataaataaa
     4081 cagcaacaaa aacatttgat aatcacttag tgattaaata caattaagac gattttgtta
     4141 taagagaggc accatggagt gagataatga ataacgaaca gatgcttatc aaatgcagag
     4201 atccccgtaa atgtcggttt tatgccgaat atctgaatca caaataccct aatgaattcc
     4261 ctcactttgc gcctgacaac ccacaaaatt gtatcaggaa tgttaattta tttttaaagg
     4321 aaaagtattg taatgtttca cctcccccct ttggtacttt tggtactact gaagaagaag
     4381 cactcaataa cgaaattaat gagcttgaaa ttattgccgg gcaggcaata ctgcctgaag
     4441 agagttttct ctggttgaaa actgacgaac ttgcgacttt ctttacctgg actactattt
     4501 atctgtcgaa ggaagaccat acaaaattcc cgaaaataaa ccataattca tcaggaataa
     4561 aaaacggaat tgaagtcagg aaaatacgaa cagttaccga tgaaacgtta aacacttaca
     4621 aaaaaagtaa ccttccggat aatccatcat cccataacga acgaataaaa gtaatcattt
     4681 catattttga ctcctgccat tctttaatta ataaaaatgc aaaaagcata tacctcgagg
     4741 aactcaggca ggcgtggctt aaaaagaaaa gcgcttgtca aaagtttaag tggctccttc
     4801 ctgacgatga tgaaatgtgt cagtggttct ggacaagact gaaacaaaaa caggatgaaa
     4861 aacatccagt acataaccca gtgacccggt ggttcatacc atcttcaacg aaagaatatt
     4921 atcttgcctc tcttgtcgcc tttgctttat ggaaagaagc gcctgatacc attgaactgt
     4981 tcagaacacg aataaatcat gcctggcatc agaaaaaaca aaaggacaaa tatcggagtg
     5041 atggtaaaaa ggccattaat atacatataa gaagcaatgt gaaagaaatg cttgatgagc
     5101 tcagaactgt ttacggtctg agaacaggag agttttttga aatgctgatt acagagaaat
     5161 atcttgaaca caagcgaagg aagtaattca ttacctgtta cattctaaat aatctcagac
     5221 agatattatc tccgtgaatc ccccgccacc tttccggtgc g
//
LOCUS       DAJTRD010000183         5172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTRD010000183 DAJTRD010000000
VERSION     DAJTRD010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.184"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..736
                     /locus_tag="OO315_003991"
     CDS             <1..736
                     /locus_tag="OO315_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5892686.1"
                     /translation="SQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVT
                     YPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLGS
                     GNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFY
                     LSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLS
                     ATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1070..3349
                     /gene="ydeP"
                     /locus_tag="OO315_003992"
     CDS             1070..3349
                     /gene="ydeP"
                     /locus_tag="OO315_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5892687.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3597..3800
                     /gene="safA"
                     /locus_tag="OO315_003993"
     CDS             3597..3800
                     /gene="safA"
                     /locus_tag="OO315_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5892688.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3868..4629
                     /gene="ydeO"
                     /locus_tag="OO315_003994"
     CDS             3868..4629
                     /gene="ydeO"
                     /locus_tag="OO315_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5892689.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5031..>5172
                     /locus_tag="OO315_003995"
     CDS             5031..>5172
                     /locus_tag="OO315_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5892690.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDF"
BASE COUNT         1479 a         1101 c         1154 g         1438 t
ORIGIN      
        1 gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg atcatataaa
       61 cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat cgctgtactg
      121 gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg atattggtga
      181 taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg cggccggtgg
      241 agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa ttgcaacatt
      301 aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca gtgttgttat
      361 gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc cggactttcc
      421 tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc tctcatttta
      481 tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag cacctgatgc
      541 aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac tggctacggg
      601 agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt taagcgcaac
      661 ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca ttggtgtcac
      721 ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc acgaaaattg
      781 cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc cgcgcacaat
      841 tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac tatgagagat
      901 gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa aatagaaaga
      961 gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat gtactgtgca
     1021 tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga tgaagaaaaa
     1081 aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg tagcgaatgc
     1141 agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca tgaataagcc
     1201 agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg cgtcattcga
     1261 catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc aggtaaatgc
     1321 ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc acgagcttga
     1381 ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact gttacaagcc
     1441 attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc
     1501 caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct ttctttatca
     1561 gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca tgtgccatga
     1621 accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg tgttgctgga
     1681 agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta caaaccaccc
     1741 tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa
     1801 tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt ttgaaatgct
     1861 gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg gtggcgatat
     1921 ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc
     1981 aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg gctttgacga
     2041 gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt ctggactaag
     2101 tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca ccattatctg
     2161 ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc aactggtcaa
     2221 tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc cactacgtgg
     2281 tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt
     2341 tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg gacatgctgc
     2401 aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct gcatgggggg
     2461 caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga
     2521 tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag
     2581 ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc
     2641 ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc
     2701 cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc
     2761 ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat
     2821 tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc ccggtggttt
     2881 tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt
     2941 cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat
     3001 ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta
     3061 tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg
     3121 ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag
     3181 ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc gctcactggt
     3241 gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc cattaagtgg
     3301 tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat gcctcttctc
     3361 atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta ttgcgcaatt
     3421 tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc ctacagctgt
     3481 aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc agcctgttat
     3541 attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata tttgaaatgc
     3601 atgcgaccac agtgaaaaac aaaatcacgc aaagagataa ctataaagaa atcatgtctg
     3661 taattgtggt tgtcttatta ctgacactta cgttgatagc cattttttcg gcaattgatc
     3721 agctgagtat ttcagaaatg ggtcgcattg caagagatct tacacatttc attatcaata
     3781 gtttgcaaga ctggaaataa acagcaaatt ataaatgtga aatgtttaaa aggtatcgat
     3841 aaaactttat tgttttaagg agataaaatg tcgctcgttt gttctgttat atttattcat
     3901 catgctttca acgctaacat tttagataaa gattacgcct tctccgacgg cgagatcctg
     3961 atggtagata acgctgttcg tacacatttt gaaccttatg agcggcattt taaagaaatc
     4021 ggatttaatg aaaataccat taaaaaatat ctacaatgca ctaacatcca gacagtgacg
     4081 gtgcctgttc ctgcgaagtt tttacgtgct tcaaatgtac cgactggatt gcttaatgaa
     4141 atgattgctt atctcaactc ggaagaacgc aatcatcata atttttcaga acttttgctt
     4201 ttttcttgcc tgtctatttt tgccgcatgc aaaggtttca ttacactatt aactaacggt
     4261 gtgctatccg tttccgggaa agtgagaaat attgtcaaca tgaagctggc gcacccatgg
     4321 aagctgaaag atatttgtga ctgcctgtac atcagtgaaa gcctgttgaa gaaaaaacta
     4381 aagcaagagc aaacgacatt ctcacagatt ctattagatg ccagaatgca gcacgcaaaa
     4441 aatttgatac gcgtagaagg ttcagtcaat aaaattgccg aacaatgtgg ttatgccagt
     4501 acatcttatt ttatttatgc gttccgcaaa catttcggca acagtccgaa gagagtttct
     4561 aaggagtacc gttgtcaaag gcacacgggt atgaatacgg gcaacacgat gagtgcttta
     4621 gctatttgat tatttgctaa cgagtagtca accacgcacg ctacgtaaga attatatggg
     4681 gcagccattc cctgccccgc gttgttttta ggcgatatat ttattgaaat aaataagtga
     4741 catccatcac atatttatgc acttgcataa cctgttgcat gattatttat gatctcaatt
     4801 cggcattttg tcagtaaaat gcaataattt attaaatatc aataaattag ttgtttatcg
     4861 gcgagaaatt acttaataga acagaaagta atgtcaacgc tttatggact gttatttccc
     4921 tttttttagc taaatctgct atctctttat gtgactaact tcacttacat ccacttattt
     4981 ctcttcgtaa aattacttcg gaattaagta caataagaag aggaacattt atgaagtctg
     5041 cattaaagaa aagtgtcgta agtacctcga tatctttgat actggcatct ggtatggctg
     5101 catttgctgc tcatgcggca gatgatgtaa agctgaaagc aaccaaaaca aacgttgctt
     5161 tctcagactt ta
//
LOCUS       DAJTRD010000184         5145 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTRD010000184 DAJTRD010000000
VERSION     DAJTRD010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.185"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            190..447
                     /locus_tag="OO315_003996"
                     /pseudo
     CDS             190..447
                     /locus_tag="OO315_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO315_003997"
     CDS             complement(497..1447)
                     /gene="uspE"
                     /locus_tag="OO315_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5892691.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO315_003998"
     CDS             complement(1599..2351)
                     /gene="fnr"
                     /locus_tag="OO315_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5892692.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO315_003999"
     CDS             complement(2546..3061)
                     /gene="ogt"
                     /locus_tag="OO315_003999"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5892693.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3072..3215)
                     /locus_tag="OO315_004000"
                     /pseudo
     CDS             complement(3072..3215)
                     /locus_tag="OO315_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3605..4420)
                     /locus_tag="OO315_004001"
     CDS             complement(3605..4420)
                     /locus_tag="OO315_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5892694.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4517..>5145
                     /locus_tag="OO315_004002"
     CDS             4517..>5145
                     /locus_tag="OO315_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5892695.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLE"
BASE COUNT         1373 a         1172 c         1226 g         1374 t
ORIGIN      
        1 gctattttac cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat
       61 tagctaacta atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag
      121 acaacggtga aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag
      181 gattacatca tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt
      241 ttgttggcgg ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt
      301 atagaacagt acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca
      361 tcgatgattt ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt
      421 tatttccttg gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc
      481 agttattgaa gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact
      541 ggtcaggttt aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt
      601 tgccgaggaa tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg
      661 cctgtaaatg ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg
      721 tcatgttttc attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc
      781 cacgaatggc atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa
      841 ttggcgttac cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca
      901 gttcgatcgt ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg
      961 cgagattcac cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc
     1021 acaccgggct tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt
     1081 ccagacggtc atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt
     1141 cctgaatgat ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc
     1201 cagcattgag ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc
     1261 cctgacgcat agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt
     1321 catagatcgg caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc
     1381 gccgcaatgc tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca
     1441 tagccataca gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc
     1501 catatctggt gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt
     1561 aatgatatga cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg
     1621 ccagcaagct gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc
     1681 agcataccgc ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc
     1741 agatagttac caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg
     1801 cgttgggcaa aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt
     1861 ttcttcgaca acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc
     1921 atcatctgct gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga
     1981 atttcacata ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg
     2041 atggcgtcaa atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct
     2101 tgctcagtga ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt
     2161 tcatcaccag ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata
     2221 ttatcaagct gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg
     2281 ctgcaatcct ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt
     2341 tccgggatca taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta
     2401 gggagcaagt aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata
     2461 tatgtctatc tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa
     2521 caagctggca caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc
     2581 actcttttcg ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc
     2641 gatggcaagg tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg
     2701 ccgcgccagg gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg
     2761 ggatagtgcg tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg
     2821 gaagcgtatc aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac
     2881 cacctgggtt ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca
     2941 gctgcaccat gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat
     3001 cgcaaatcac ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca
     3061 tccgttctct cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga
     3121 taccacgcca gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact
     3181 aacgaatagt aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga
     3241 ttttttaatt tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga
     3301 taattccagt cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata
     3361 gccgccagac accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt
     3421 tacagagcta aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt
     3481 acacacagca gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt
     3541 gttataacgg ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata
     3601 taagttaaat catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt
     3661 taccgggcga gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat
     3721 agcccagacg atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag
     3781 aaataacgtg catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata
     3841 agcgcatgag tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca
     3901 gatacccttg gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc
     3961 aagggagttc aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga
     4021 gggctgaaaa acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa
     4081 ttgcaattag taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca
     4141 gcgtttcact ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa
     4201 caaacttatg cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga
     4261 cgctttctcc agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga
     4321 gatgtttatg ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt
     4381 gatgccctgg tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca
     4441 gaaatcatca aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata
     4501 tacggtgtaa agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg
     4561 tattcttttg actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca
     4621 gacgacggat gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg
     4681 ttatatttct gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct
     4741 gcgtctcgat gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag
     4801 tagcaaagcc aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca
     4861 aagtgccagt gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac
     4921 tgaacgttat gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc
     4981 caggtttgac taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt
     5041 ggagaagcaa cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga
     5101 agaagttcag gccgctttga ctcaggcact actggatctg gaata
//
LOCUS       DAJTRD010000185         5140 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTRD010000185 DAJTRD010000000
VERSION     DAJTRD010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5140
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.186"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(363..608)
                     /locus_tag="OO315_004003"
     CDS             complement(363..608)
                     /locus_tag="OO315_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892696.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1385..2227)
                     /locus_tag="OO315_004004"
     CDS             complement(1385..2227)
                     /locus_tag="OO315_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR5892697.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2312..2509)
                     /locus_tag="OO315_004005"
     CDS             complement(2312..2509)
                     /locus_tag="OO315_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892698.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2529..3017)
                     /locus_tag="OO315_004006"
     CDS             complement(2529..3017)
                     /locus_tag="OO315_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892699.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(3014..3391)
                     /locus_tag="OO315_004007"
     CDS             complement(3014..3391)
                     /locus_tag="OO315_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5892700.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3438..3815)
                     /gene="cbtA"
                     /locus_tag="OO315_004008"
     CDS             complement(3438..3815)
                     /gene="cbtA"
                     /locus_tag="OO315_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCR5892701.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3895..4116)
                     /locus_tag="OO315_004009"
     CDS             complement(3895..4116)
                     /locus_tag="OO315_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5892702.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4203..4679)
                     /locus_tag="OO315_004010"
     CDS             complement(4203..4679)
                     /locus_tag="OO315_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5892703.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4695..>5140)
                     /locus_tag="OO315_004011"
     CDS             complement(4695..>5140)
                     /locus_tag="OO315_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5892704.1"
                     /translation="SACAPETDLQQLVATLVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         1198 a         1247 c         1363 g         1332 t
ORIGIN      
        1 tgtccactat tgctgggtaa gatcaggctg aggagtcccc agaaaagaag acaggcatag
       61 agttttgtga tctactgatt gcgctaacaa attcaatgag atcacctcta tgcctgcccg
      121 ttaagtatgt caggcttttt tccgtaatgc tctaacttcc acgcattggt ttctgacatc
      181 aaataaggct atgactttct ctccctttta atctcattct gatctaaaac catatcttaa
      241 aaaaatgcat gggtatttta attattttga tgttgatcta acgcactcaa ggaccatttt
      301 attaccgatt atccattcta tttaactgtt gcgatataaa tgtttgtttt aggtgcttta
      361 attcagtaca agtcatcaaa ccgtaccaga ccctgactgt cagtgacacc gacaggtggg
      421 ggcggcggtg tggggtcaaa ctgagtgtgt ctgcctctgg cccacagagc cagctctgcc
      481 gccattgccc gtggatgata ctccagctgc acgttgagac cggaaactgg gttgttgttc
      541 tcatccacga actgcagcgc cagccagtgc atccgctgct taccgcaggc gacttctttg
      601 tacttcattt tgtgactcag gcaggtgact gtcgtgttgc ctgttgcgtc cattttgcgg
      661 ttaaaccctt ttaattcaat ttgttaaatt gctttccacc ttttgcgggg cgcgacttaa
      721 ccaaaatatt cactgttatc ataatatgca atgggctgga ggttgcaggt catttaggga
      781 ttttctgtaa gccccgtttt gcagaacgtt ctatctccga ttgagatcct gtctctcggt
      841 attaccggta gtctggccat aatcccctat ggatcatatt gatatagtag gcataacacc
      901 caaaagttga ctaaagctaa atcaatcaga gtgaaaaatg ttcggtttga tgttgggggg
      961 gactgaggag gggccaacgc aggtggtatg cgttttactt cagaccggac aaagaccgga
     1021 tgtatccgtg cagtaatgaa tccgttagcc aggtgctcat gtggaaatag tgtctattgg
     1081 ggatttgaag tttgacagaa aaggccacag tgaccagaag ggggctactt agaagaaatg
     1141 ttcaggctgg gtggaagagg taaagcagtg acatcagcat tcaaagtgac cggatatcta
     1201 ccagaacccc agaaagcaaa aaaccagccc gtaggctggt tcttctaaat agtggtgccg
     1261 gactcggaat cgaaaatttc ttttatcata ttgatattaa tgcaatttgt ttggtttgat
     1321 taaaaatgta ccctcaatta tacccgcagt cccttttgat atcgaaaggg aatcactctc
     1381 gggatcaggc tgagagcaca gaaggaaagt acctggctat tatatcgtta attctgtcaa
     1441 tcagttccag ccttttgaaa gtgatgtgtg cagatccctt cttaaagtat ttaatgatga
     1501 acatctcgtc ttcatagcgc tctttaccct gaacggactg gatgtgacca tccagacgaa
     1561 tagtgatgtt ttcccggttg tcggggatcg gtttgccgct gaacagatgt agcattcttt
     1621 ccaggtcgac gagtcggtca gtctgttgcc cggtgataag atgaaacccc caccggtccc
     1681 acctcaccag gttgttgacg ataattttac tgccaaattt acagggtgag ttggttttgt
     1741 aatcccagct cagccctttg aagacgttga tcactccccg ttcaaacact tcatctttgt
     1801 tctggtgcag ttgttcaaat gtgctcagaa tgttcgcttc gctgatttcc ggaaaattat
     1861 catattccag tgacctgtac catgtgtccc gggtctgcgc atccatcaga ctcagcatcc
     1921 cggaacgttg catcagatca cgccagagtt cgcgatcaag attgcgtgtg atggctttca
     1981 ttgcggtttc cggtttctcc atcagccagc aaccgcagcg aaaatcctgt ttcatggccc
     2041 agtccagagc cgttttacct ctaatacttt ctgtcattgc tgatacttca gccagttgtc
     2101 ggataagtgt gtcgatttgc agcaatgtac tctttcgtac agccagaatg tggcggatac
     2161 tggttgagca gataacgtcc gtatgcccgt tgaccacttc cggacgagag gctgtgattt
     2221 cagacataat cgatattcct ttttgcagat aaacgaaaac gccagccgta ttcaggctgg
     2281 cgtcagtgga atgaagtctg gtgagtaaga ctcaccggtt ctgttgcaga aactgcagct
     2341 gacgcagggt acggatatct tccctggcct gttcaataca gggcagcagt gctgccgaat
     2401 ccgttttatc ctcatcgttg tcgaagataa tccctgattc gcagtcaata ttgtcctgca
     2461 gccacgcaat cagaatatcc agcgccgttt ctgtggttaa taatttcatc cagtgtcttt
     2521 tgtcctgatt accagtcgaa agtggggaac cccggcagac attctgcgcc ggcgtccaga
     2581 tgaatgagac tgacgccata acgccgggtg agagtgacga ccagacgacg gaacgttctg
     2641 gacaggccca ggcgtttaag ccgcagaacc gggtacgacc atgcatcggt acgtaacaga
     2701 tagccgctgc ctgtaaaatg aatccattct gtttcgccgt cgtcattagt ctggtgtgac
     2761 agcgagtaca gccaggcgtt gtccgtttcc gtgatatgtg cggtgctgca acgtatgcag
     2821 gtaagggttg ttaacggtgg tggtgtactg gttgcgtgtt ggtcggattt atcgactacg
     2881 cggagtgaat aaccgttttc agagaccttg ttaatcagtt cactgagatt aacaccatcg
     2941 acgtcaacga caatgcgccc catgttcagt gcctgcacgt taacgctgtc ggcttccagc
     3001 gtcagggcca gtttcattgt ttcgcttccg gatgcttccc ccgggtaatg ttatttaccg
     3061 ttctgtagtt atcgcgggtc atcaggccgg ttgcccggcg tgcccggaga atatcaatgc
     3121 tgttgattaa ctgcgacggg gctcctgcgc tgaatcccgg ctggtcggta cgtaccagcg
     3181 cgtatttttc aacgagaaag ttcaccgcgt cacacagtga aattcctgcc tcaatatgct
     3241 gctcaatcac acgttcatca gcgaacggtg tgtcattcag cgtgaggcca tagtgctggt
     3301 ccagcagtcg ggtgagcagt gtctgccaga tggtgatggg agacgggcag cccgatgcct
     3361 cccgtacgtg agtgtcgggt aatgtattca tcttaagttc tctgtaacag gtggttataa
     3421 aggggagata tccggtgtta tacggtggtt gcgggcgctg ccggtgtcgg ataaacagcc
     3481 agataaacgt aaccacagga gccgagggtg tcggcttcgc aggtcagccc tttcgcatac
     3541 agtgtgacgg tatgctgatg gcggggattc agttcaccgc cagtgagcat gagttccagt
     3601 tgtttcatca gcagcggaaa ggcctggtcc aggtggtacg catctacgtc gctgaacctg
     3661 cctctgatac cggcgcggtc tgcaaggtaa tgcaggcggt taccctcctg taccagtcgg
     3721 gccccgaaac agggcgtcac ggtgcagggc agcccccacc aggggcggtc gtgattgtcg
     3781 tcgggaggcg ttgtcccggg gagtgtgtct gacacgataa agtcctcaca gaaaatcggt
     3841 taagaatggt ccggtagtgg cgataattct gctcatcaga attcccttcc agactcagtt
     3901 cagggtgacg ctcatcagcc ggacatacgg accaaaactg tccttacggc gttctgcaaa
     3961 cacagccagt acaccgggaa tatcctgcac ttcacggccg gtatatgtat ccgtgctacc
     4021 gtgccagcgg tatttaccgg tacagaacgg aaaaagacgg gatgcaggat gctgacggtg
     4081 aatacgcatg gcttcaccac gggtgatgat tttcatgaga atagtcctcc tgaatgtgaa
     4141 agtaagaaac agctgagaaa gagaaactga agtgatatga aggtgattgt gacggtgacg
     4201 ggttaaagca gaccgtgttc cgcaaaggag aaaacctggc tgccaccgac tatcagatgg
     4261 tccggtaccc ggatatcaac cagggccagt gcctgtacca gacgctcagt gataaggcgg
     4321 tctgccttgc tgggggggac ttcaccggac gggtgattgt gcgccagcac cacggcagcg
     4381 gcattgtggt acagggcgcg tttaatcact tcccggggat ggacttccgt acggttgatg
     4441 gtgccggtga agagggtttc accggcaatc agctgattct ggttgttcag atacagcacc
     4501 cggaactctt cacgctccag tcccgccatg ttcagaatca gccattcccg tgccgcatgc
     4561 gtggaggtga aggccacgcc gggttcatga agatggcggt ccagggtttt cagggcccgc
     4621 agaatgaggc tgcgctctct ggttgtcatt tctccgggca gaaaggaaag ctgctgcatt
     4681 gtccctgcca ccattcagtc gatgatgcgc ataatggcgc tgcattccgg atgctgcagg
     4741 gcgtaatccc gcagccggta ataatggacc gtcatggcat aacactccgt acgacaggca
     4801 tgatgactgt acgtcatcag acaggcagca atgccggcag cttccggact catttcagcg
     4861 cggttaccgt tcatggcatt gaacagtacc catgtttcgt catcatcgtc atccggttcg
     4921 ggtgccataa atgccccgcc gttgttcagg gtgtacagat tccagatacc accgcagtag
     4981 tcttcgcaca gacggtccat ccagccgaag acacggggct ccagggtcac ccactgtgga
     5041 atgaggccaa agtgctgcgg ccagaagctg atgcgctgtt catcagggac gagggtggca
     5101 accagttgtt gcaggtcagt ctccggtgca caggccgaag
//
LOCUS       DAJTRD010000186         5024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTRD010000186 DAJTRD010000000
VERSION     DAJTRD010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.187"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="OO315_004012"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="OO315_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5892705.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="OO315_004013"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="OO315_004013"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5892706.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="OO315_004014"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="OO315_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5892707.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="OO315_004015"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="OO315_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5892708.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5024
                     /gene="yidZ"
                     /locus_tag="OO315_004016"
     CDS             4378..>5024
                     /gene="yidZ"
                     /locus_tag="OO315_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5892709.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDT"
BASE COUNT         1144 a         1208 c         1256 g         1416 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctg
//
LOCUS       DAJTRD010000187         4876 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTRD010000187 DAJTRD010000000
VERSION     DAJTRD010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.188"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(89..673)
                     /gene="kptA"
                     /locus_tag="OO315_004017"
     CDS             complement(89..673)
                     /gene="kptA"
                     /locus_tag="OO315_004017"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5892710.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            923..1606
                     /locus_tag="OO315_004018"
     CDS             923..1606
                     /locus_tag="OO315_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5892711.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1603..2064
                     /locus_tag="OO315_004019"
     CDS             1603..2064
                     /locus_tag="OO315_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5892712.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2077..3249
                     /gene="iadA"
                     /locus_tag="OO315_004020"
     CDS             2077..3249
                     /gene="iadA"
                     /locus_tag="OO315_004020"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5892713.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3314..4225
                     /gene="hypT"
                     /locus_tag="OO315_004021"
                     /gene_synonym="qseD"
     CDS             3314..4225
                     /gene="hypT"
                     /locus_tag="OO315_004021"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5892714.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4218..4610)
                     /gene="iraD"
                     /locus_tag="OO315_004022"
     CDS             complement(4218..4610)
                     /gene="iraD"
                     /locus_tag="OO315_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5892715.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(4607..4690)
                     /gene="idlP"
                     /locus_tag="OO315_004023"
     CDS             complement(4607..4690)
                     /gene="idlP"
                     /locus_tag="OO315_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5892716.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         1151 a         1279 c         1220 g         1226 t
ORIGIN      
        1 agggtatccc ttcagactga taacaaacgc tacattgccg gatgtgctgc gcttaccagg
       61 cctatataac ccctgcaata tattgaattt attgtttttg taccccagtc ggcataaacg
      121 accactcaag aaactcaaca gcaaccgttg atgtcagcca gaccccgttt tccgcttgcc
      181 agaaagggat gcctcgtttc gccatttcct gtgctttgac ggttaagaca accggcgatc
      241 cgtgtcttgc tcccactttg cgtgccgttg cttcatcagc agagagatgc acgtaatgac
      301 gatcccctgc aatcagcccc tgttttttta tctcgtcaag gaaacggctt gccgtaccgt
      361 ggtagagaaa ctgtggcggt gttttttcgg caaaggaaat agcgacctgc gaagtcgaat
      421 gcccctgaac cgcgcggatg cacctaccat cactggaata actaaaacgc tttttatcgc
      481 tggttgcgac cacggtatcc agcaatgcac gggttaatcg cttgcccgct ttttgggcac
      541 aaagtatgag cttattgata tccgcccatc cttcacggtc cagtacgata ccaatcgcct
      601 ccggtttgtg tcggaggaca aaacttaaaa acttgctcgt atccgccagc tctttttcgt
      661 tgtactttgc catcgcgttt tcgtccctgg attttctgtt tttatttact aatgtcattg
      721 tcactgggtt tatgaatttt ctgcaactca atcctgcgca gaaccttttc cacacagcgt
      781 aatttttccc ctatgcaaaa aacgcatagc cagaaacata cttgcatttt ccccataccc
      841 acatggctga taaggttttt cagttccagg aattttcctg gctcaagcaa cacacaaaca
      901 caaaaaaaca atggggattg ttatgacaca acaaggggat gctgtagccg gggagctggc
      961 gacagaaaaa gtgggcatta aagggtatct ggcttttttt ctcaccataa tcttttttcc
     1021 cggtgttttt tccggaacag acagctggtg gcgcgttttt gatttttccg ttctgaacgg
     1081 atcgtttggg caactgcctg gtgcgaatgg agccaccacc tcatttcgcg gggtgggtgg
     1141 cgcgggggct aaagatggct ttctctttgc gctggagctg gcaccgtcag tcattctctc
     1201 tctggggatt atttcaatta ccgatggtct tggcggatta cgcgccgcgc agcagttaat
     1261 gacacctgtg ctaaaaccgt tgttgggcat tccagggatc tgctcgctgg cactgatcgc
     1321 taacctgcaa aacacggatg ccgccgccgg tatgaccaaa gagctggcgc aggaaggtga
     1381 aattaccgaa cgcgataaag tgattttcgc cgcatatcaa accagcggta gcgccatcat
     1441 caccaactac ttctcttccg gcgtcgcggt gtttgccttt ctgggcacgt ccgtaatcgt
     1501 acctttagcc gtcatcctgg tgtttaaatt tgtcggagcc aacattttgc gcgtctggct
     1561 taacttcgaa gaacgtcgca acccaacgca aggagcgcaa gcatgacaac tcaggtccgc
     1621 aaaaacgtca tggatatgtt tatcgatggc gcgcgccgtg gctttactat cgccacgacc
     1681 aacctgctgc caaacgtggt gatggcgttt gtcatcattc aggcgctgaa aattaccggc
     1741 ctgctcgact gggtggggca tatctgtgaa cccgtaatgg cgctgtgggg attacccggt
     1801 gaagcggcaa ccgtcctttt agccgcatta atgagtatgg gcggcgcagt cggtgttgcc
     1861 gccagtctgg caaccgctga cgcattaacc ggccacgatg tcaccgtttt actgcccgca
     1921 atgtatttaa tgggcaaccc ggtgcaaaat gtcggtcgct gcctggggac agccgaagtg
     1981 aatgccaaat attacccgca tatcattacg gtctgcgtga ttaacgcatt gctgtcgatc
     2041 tgggttatgc agcttattgt ttaacaagga gttaccatga ttgattatac cgcagccggt
     2101 tttaccctgc tgcagggagc gcatttgtat gcgccggaag atcggggaat ttgcgatgtc
     2161 ctcgtcgcta acggcaaaat tatcgccgtt gccagcaata tcccttctga cattgtaccg
     2221 aactgcacgg ttgtcgatct cagcgggcag atcctctgcc caggttttat tgatcaacac
     2281 gtccatttga ttggcggtgg cggcgaagca ggtcccacga cgcgcacgcc ggaagtggcg
     2341 ctaagtcgcc tgacggaagc gggcgtcacg tcagtggttg gtctgctggg caccgactct
     2401 atctctcgcc acccggaatc cctgctcgcc aagacccgtg cgctcaatga agaaggcatc
     2461 agcgcctgga tgctgaccgg cgcttatcat gtcccttccc gcactattac gggttccgtg
     2521 gaaaaagacg tggcgattat cgatcgtgtg attggcgtga aatgcgccat ctctgatcac
     2581 cgttctgccg caccggacgt ttatcacctg gctaatatgg cggcagaatc ccgcgttggc
     2641 ggtttgctcg gcggtaaacc tggcgtcacc gtgttccaca tgggcgacag taaaaaggcg
     2701 ttaaagccta tttatgacct gctggaaaac tgcgatgtgc cgatcagcaa gctgctgccg
     2761 acccacgtta accgcaacgt accgttgttt gagcaggcgc tggagttcgc gcgcaaaggc
     2821 ggcaccatcg atatcaccag cagcattgac gaaccggtcg cccctgccga aggtattgcc
     2881 cgcgccgttc aggcgggtat tccgctggca cgcgtcaccc tcagctccga cggcaacggt
     2941 agccagccgt tcttcgatga cgaagggaat ttaacccata tcggtgttgc aggttttgaa
     3001 acgttgctgg aaaccgtgca ggtgctggtc aaagactatg atttcagtat cagcgatgcc
     3061 ctgcgcccgc tcaccagtag cgtagccggt ttccttaaca tgagcgggaa aggcgaaatt
     3121 ctgccaggca atgacgcaga cttactggtc atgacgccag aattgcgcat tgagcaggta
     3181 tacgctcgcg gcaaactgat ggtcaaagac ggcaaagcct gcgtgaaagg aacgtttgaa
     3241 acggcttaaa cggttgtcag ccgccctaat ggacgtagaa tgcccccaag ggcggctgac
     3301 agagtaaaac gtaatggatg actgtggtgc ggttttgcat aatattgaaa ccaaatggct
     3361 ttatgatttt ctgactctgg aaaaatgccg caatttttcc caggcggcag tcagtcgcaa
     3421 cgtctcgcaa ccggcattca gccgccgcat ccgtgcactg gaacaggcga ttggcgttga
     3481 attgtttaac cgccaggtga cgccactgca actctcagaa caagggaaaa tcttccattc
     3541 gcagatccgc catctgttgc aacagttaga aagcaacctt gcagagctgc gtggcggcag
     3601 cgattacgcg caacgtaaaa tcaagatagc cgctgcacac tctctttccc tcggtctgtt
     3661 accgtccatt atcagccaga tgccgccgct ctttacctgg gcaattgaag ccattgatgt
     3721 cgatgaagcg gtcgataaac tgcgtgaagg gcaaagtgac tgtatttttt cctttcacga
     3781 cgaagatttg ctggaagcgc cgtttgacca cattcgctta tttgaatctc aattgttccc
     3841 tgtctgcgcc agtgacgaac acggagaagc actttttaac ctcgcgcagc cacactttcc
     3901 gttactgaat tacagccgca actcctacat ggtgcgattg attaatcgca ccctgacgcg
     3961 ccacagtgag ttaagtttca gcaccttttt tgtctcttcg atgagcgagc ttttaaagca
     4021 ggttgccctc gacggctgtg ggattgcctg gctgccggag tatgccatcc aacaagaaat
     4081 tcgcagcggg cagctcgttg tgctgaatcg ggacgaacag gtgatcccga ttcaggccta
     4141 cgcataccgg atgaataccc gcatgaatcc agttgccgaa cgcttctggc gtgaactgcg
     4201 cgagctggag attgtgctta gctgacattc tcaagcgtcg cactgcgcca accgccatta
     4261 tcccagacca gatcaaacgt tagcggttca tcgtcgtggt acagcattat ctcaaggctg
     4321 atcgccagag tgtggcagta cgcttctttc aaattaacct gcaacgccgc aatccgtggc
     4381 tcatgaaata acagtgcaga acgcagctga cggcaatacc agctctggag atgtttgtcg
     4441 ctcgggttat gcacttgcca ctcgggcatg cctgaaacaa gtaatgacgg taatgccgaa
     4501 tgaggagaat taaaaagagt gaggagatcg ctgcataccg atttacgcag caatgacgtt
     4561 ttattgcctg aaatttcagg taaaacagcc tgaagtgatt ggcgcatcat tttgcgcact
     4621 cctgacgttt agcaacatat gcggcttgcc ctgaacaccg ggcaccactg tattgatgct
     4681 cattttccat agccattccc ttttgttcat tacatccatg tcagtctgag acaggcctga
     4741 atcctgtcag caaagtataa aaccttactt gcctatgaat atctactgac ttttcaggaa
     4801 aaaagtcatg atttgataaa aatcatcaaa ctcaaaaaaa aaacgctagt atattctgaa
     4861 tattccgaat agttac
//
LOCUS       DAJTRD010000188         4826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTRD010000188 DAJTRD010000000
VERSION     DAJTRD010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.189"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            109..293
                     /locus_tag="OO315_004024"
                     /pseudo
     CDS             109..293
                     /locus_tag="OO315_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            436..657
                     /locus_tag="OO315_004025"
                     /pseudo
     CDS             436..657
                     /locus_tag="OO315_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(678..740)
                     /locus_tag="OO315_004026"
                     /pseudo
     CDS             complement(678..740)
                     /locus_tag="OO315_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1190..2602
                     /gene="ospC1"
                     /locus_tag="OO315_004027"
     CDS             1190..2602
                     /gene="ospC1"
                     /locus_tag="OO315_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5892717.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(2706..2856)
                     /locus_tag="OO315_004028"
                     /pseudo
     CDS             complement(2706..2856)
                     /locus_tag="OO315_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            2932..4629
                     /gene="ospD3"
                     /locus_tag="OO315_004029"
     CDS             2932..4629
                     /gene="ospD3"
                     /locus_tag="OO315_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5892718.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
     gene            4746..>4826
                     /locus_tag="OO315_004030"
     CDS             4746..>4826
                     /locus_tag="OO315_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5892719.1"
                     /translation="MEYRTWITEALRLHFEEHLPRVVAGRR"
BASE COUNT         1672 a          877 c          901 g         1376 t
ORIGIN      
        1 tttagctgaa cgggccatat aaccacctga aagacaatga cattgcctgt ttttataacg
       61 gtaattgcag accatgacaa accgcagccg tcaggctgcc tactcgagtc acaaggctct
      121 ttgtgtccga tatgccggca gcgcattacc catgaaactg gctggaacgt acaccataag
      181 gtgaagaaag ttatgggggc ggtgaagaat tatctaacct tgtcttgtta catccaaatt
      241 gccatcgaca gttacacagt tgtgaagccg gctctctcaa aaagggctta taaaggcttg
      301 agcggtgtac cgggaaaccg gtatgcaccg ttcttagggg agggcagtcc agcaatgaac
      361 tgtccttacc cgacaaacat ccaaaacgaa cggaacgtgc tggaatccgc atataacccg
      421 ttgtaaagtg aggaaatgga cacctcactt gctcatgaga acgcccgcct gcgggcactg
      481 ttgcagacgc aacaggacac tatccaccag atggctgaat acaaccgcct gctctcacag
      541 cggatggcgg cttatgcttc cgaaatcaac cgactgaagg cgctggttgc gaaactgcag
      601 cgtatgcagt tcggtaaaag ctcagaaaaa cttcgcgcaa aaaccgaacg gcagattcca
      661 ttttcacggg cgatctacgc cacacaggct ctgggttgca gtgactgctc cattaaggcg
      721 attttgaact cataagggaa gtaacaaaaa aactgcaaaa gtacaataat tatatatggc
      781 caaactaacc tctattaaaa aacatcagtt tttcacactt agttgagctt catgaaaaaa
      841 tcatcgagga tttcattgca gaatacacat tccactaact ggaagcactc cataagagga
      901 atcagagaaa gtatcgtttt tttatagtaa aattcattgc tgttcaataa aattgatata
      961 gttattaaat tatgaaaata atttcacaaa aaagattaac ttgagttctt aagcatcgga
     1021 ttttagatat tttgaaatat ctggcatcac ataacatcaa aagtatataa tactcttatc
     1081 taattacatg cagtatattg tatatatact caaaaattgt aattcatacc ctgctatatc
     1141 ttgtagggaa agatattaat taaaactgtt ttcatataag gttcatttta tgaatatatc
     1201 agaaacactg aactcagcaa atacccaatg caatatagat tctatggata acagattaca
     1261 tacattgttt ccaaaagtga catcagtgcg aaacgctgca caacaaacta tgccagatga
     1321 aaaaaattta aaagatagtg caaatattat taaaagtttc tttaggaaaa ctatagcagc
     1381 acagagttat agtagaatgt tctctcaagg ctctaacttt aaatctttaa atatagcaat
     1441 tgatgcacca tcagacgcta aagcctcatt taaggctatt gagcaccttg acagattatc
     1501 gaagcattat atatctgaaa taagggaaaa acttcatcct ctttctgcag aggaactcaa
     1561 tttgctttcg ctaattatta attctgattt aatcttcaga catcaaagta attctgattt
     1621 gtctgataaa attttaaaca ttaagtcatt caataaaatt cagtctgaag gaatatgcac
     1681 aaaacgaaac acatacgctg atgatataaa aaaaatagct aatcatgact ttgtgttttt
     1741 tggcgttgaa atctctaacc atcagaaaaa acaccccctg aatacaaaac atcacactgt
     1801 tgattttggt gcaaatgcgt atatcattga tcatgactct ccatatggat atatgacatt
     1861 aaccgatcac tttgataatg ctattccacc tgttttttac catgagcacc aatcattttt
     1921 agataaattt tcagaggtta ataaagaagt tagtcgatac gtacatggaa gtaaaggaat
     1981 tatagatgta ccaatattca atactaaaga tatgaagtta gggctcggat tatacctgat
     2041 tgactttatt agaaaaagtg aagaccaaag cttcaaggag ttttgctatg gaaaaaatct
     2101 tgcccctgtg gatctggata gaatcataaa ctttgttttt cagccagagt accatatacc
     2161 taggatggta agtacagaaa acttcaaaaa agttaagatt agagaaatat ccttagagga
     2221 ggctgttaca gcatctaatt acgaagaaat taacaagcag gtcactaaca aaaaaattgc
     2281 tctccaggct ctttttcttt cgattactaa tcaaaaagag gatgtcgcct tatatatatt
     2341 atctaatttt gagataacta gacaagatgt tatttccata aagcatgagt tgtatgatat
     2401 tgagtatcta cttagcgctc ataattcaag ctgtaaagta cttgagtatt ttatcaataa
     2461 gggattggtt gatgtaaaca caaagttcaa aaaaactaat agtggggatt gtatgttgga
     2521 taacgcaata aaatatgaga atgcagaaat gataaaacta ttattgaaat atggtgcaac
     2581 atctgacaat aaatatattt aatcaaaatt gaatatcgtt tagcaaaagg cagtatttgc
     2641 acaaaatatc gatatattgt ttattgtcag tatggctcaa tgtgataata gttggaaagt
     2701 ttgatgggtt tcgccccgtt gtagcggtag tcgaactgga aggtcttcag gcactgctta
     2761 cagcgataga gcagcccccc ctgaactgga atggccgttc cggatacccc cctgagtttc
     2821 agagtaacgg tgacaaacca catcaatctt tgccatcaat catccaaagg gcaaagagta
     2881 caacaacact aagtctgcgt cacaacccat caatgaaagg aatatataca tatgccatca
     2941 gtaaatttaa tcccatcaag gaaaatatgt ttgcaaaata tgataaataa agacaacgtc
     3001 tctgttgaga caatccagtc tctattgcac tcaaaacaat tgccatattt ttctgacaag
     3061 aggagttttt tattaaatct aaattgccaa gttaccgatc actctggaag acttattgtc
     3121 tgtcgacatt tagcttccta ctggatagca cagtttaaca aaagtagtgg tcacgtggat
     3181 tatcatcact ttgcttttcc ggatgaaatt aaaaattatg tttcagtgag tgaagaagaa
     3241 aaggctatta atgtgcctgc tattatttat tttgttgaaa acggttcatg gggagatatt
     3301 attttttata ttttcaatga aatgattttt cattccgaaa aaagcagagc actagaaata
     3361 agtacatcaa atcacaatat ggcattaggc ttgaagatta aagaaactaa aaatgggggg
     3421 gattttgtca ttcagcttta tgatcccaac catacagcaa ctcatttacg agcagagttt
     3481 aacaaattta acttagctaa aataaaaaaa ctgactgtag ataattttct tgatgaaaaa
     3541 catcagaaat gttatggtct tatatccgac ggtatgtcta tatttgtgga cagacatact
     3601 ccaacaagca tgtcctccat aatcagatgg cctgataatt tacttcaccc caaagttatt
     3661 tatcacgcga tgcgtatggg attgactgag ctaatccaaa aagtaacaag agtcgtacaa
     3721 ctatctgacc tttcagacaa tacgttagaa ttacttttgg cagccaaaaa tgacgatggt
     3781 ttgtcaggat tgcttttagc tttacaaaat gggcattcag atacaatctt agcatacgga
     3841 gaactcttgg aaacttctgg acttaacctt gataaaacgg tagaactact aactgcggaa
     3901 ggaatgggag gacgaatatc gggtttatcc caagcacttc aaaatgggca tgcagaaact
     3961 atcaaaacat acggaaggct tctcaagaag agagcaataa atatcgaata caataagctg
     4021 aaaaatttgc tgaccgctta ttattatgat gaagtacaca gacagatacc cggactaatg
     4081 tttgctcttc aaaatggaca tgcagatgct atacgcgcat acggtgagct cattcttagc
     4141 ccccctctcc tcaactcaga ggatattgta aatttgctgg cctcaaggag atatgacaat
     4201 gttcccggac ttctgttagc attgaataat ggacaggctg atgcaatctt agcttatggt
     4261 gatatcttga atgaggcaaa acttaacttg gataaaaaag cagagctgtt agaagcgaaa
     4321 gattctaatg gtttatctgg attgtttgta gccttgcata atggatgtgt agaaacaatt
     4381 attgcttatg ggaaaatact tcacactgca gaccttactc cacatcaggc atcaaaatta
     4441 ctggcagcag aaggcccaaa tggggtatct ggattaatta tagcttttca aaataggaat
     4501 tttgaggcaa taaaaactta tatggaaata ataaaaaatg aaaatattac acctgaagaa
     4561 atagcagaac acttggacaa aaaaaatgga agtgattttc tagaaattat gaagaatata
     4621 aaaagctgaa tattatctcg tggtgataca ctgaatttga ggtaaccggc tcatttaaac
     4681 cgtctggtct gttacctccg gttccacaaa aataatgtcc atcattttga agtggacact
     4741 atcgtatgga ataccggacc tggatcactg aagctttacg ccttcatttc gaagaacatt
     4801 taccacgggt tgtggccgga cgtcgc
//
LOCUS       DAJTRD010000189         4822 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTRD010000189 DAJTRD010000000
VERSION     DAJTRD010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.190"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(52..381)
                     /locus_tag="OO315_004031"
     CDS             complement(52..381)
                     /locus_tag="OO315_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5892720.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(386..571)
                     /locus_tag="OO315_004032"
     CDS             complement(386..571)
                     /locus_tag="OO315_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5892721.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(568..3207)
                     /locus_tag="OO315_004033"
     CDS             complement(568..3207)
                     /locus_tag="OO315_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5892722.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3415..4404
                     /gene="ldhA"
                     /locus_tag="OO315_004034"
     CDS             3415..4404
                     /gene="ldhA"
                     /locus_tag="OO315_004034"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5892723.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4515..>4822
                     /gene="hslJ"
                     /locus_tag="OO315_004035"
     CDS             4515..>4822
                     /gene="hslJ"
                     /locus_tag="OO315_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5892724.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     "
BASE COUNT         1217 a         1258 c         1122 g         1225 t
ORIGIN      
        1 tagatcaatc gctttctcct gtagcgttat cccgatattg cggttttgct attaaaactt
       61 tcgcacccat ttaccgttta tcccttgtac atattccccc ggtttggccc gagctacgag
      121 cttttgcccg gcgagtttcg cgatatcatc tacagacaca ttattttgct ttgcccgttg
      181 ttggtaactc gctttacgtt cggcattaat ttcggttact aatttctctg tttcagcatc
      241 cgttttcagc gcaaccagat aaccataaaa tgtttcacct acccgcccct gagttctggc
      301 ttcatccaga gtcaatgcca ttacattgct gcttaccagc ccaacaagaa acgcacaaag
      361 aagtaatgtt ttcttcatca tcgcctcaga aaagatcgct acgagtttca agcagctctt
      421 cgacatcttt gtctgccttg atgatgatct catgctcaat tttaacgttc atattgatag
      481 tgatcggttc cttaggtgca gcgacttcaa tgcgaggcgt acagccaacc agcataaaag
      541 atgacgtcaa cgcagccagt aaaattttca ttgtttttcc tcacactctt ttccttgcgg
      601 acagtcattt cccggcagac gtgcgttctg ctccaaccat gcctggagat tatcgccaaa
      661 gcgtaaactg cgccacagcg taaaaatatt ctcttcatga tggtaattaa ggtttaccgt
      721 accgctttta ccatcaacgc gactggtacc tgtaatgttg gcctgcatgg ttaataaacc
      781 gagattatct aagttaattt ttgtcgatga ccggctaatt tccatatagc gcaaccagtt
      841 aattgctgaa cccgcagtca tattgtcttt caccaccgca tctgcggtat ctttatccag
      901 ccgcaaggtc attagcccgc tatttgccag ccaaccatct ttcactatcc atttttcgtt
      961 gtttagccat aacggcaatg caccactaaa tgccccggac atggcgaatt gtttcggatt
     1021 gacggcgcta accagttcgc tggatgagag attattcagc cgcaacagcg ccgggtcatg
     1081 ttgcggcata cgtaattgtt tcatcagcac gttaccgcct aacacatcga cgctaacatc
     1141 agtcaacagc aagggttctt cttcagtcca cggataacgc ccttgcaaat cagccgtaat
     1201 atttttcgct gtcaccagat taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt
     1261 ccccagatgc caggctccat cggcaaaacg gaaaggcagg acaaaatcga caccgttaac
     1321 ctgattatct ggcatccagg cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa
     1381 tccttgttca ggcgcagcag aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt
     1441 catcttccag tcgggtggca ccagcggctg gaatacagtc agtgattgtt ttggccacca
     1501 ggcgttaccg cgcagacgaa taccgtccca gcgaccattt acccgaaccg ggccaatctc
     1561 accagcatgt aaatcgcctt taaagaggaa ataggtcgga ttgcgcccat cgacgctaaa
     1621 ttttaaggtt gatggcggta acacactgcc gccagtgaac agcgtttgcc cggcgtccag
     1681 tgacagcgcg ccgctgaagg agggatgctg tgcgtcacgt acccagacga cgggcttgtc
     1741 gagaattaat cgcggctttt ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt
     1801 ggagagatcg gtcagcgtaa tcgtgctgtc atgccactca ccttttcctg cgacatccca
     1861 ggtggcattc atcggtgtaa aactcccttt tccccagtag cgccactgcc agcggccagc
     1921 gtcagggaga aaatcattcg ccagcccatc catatgcagc acgaaatcgc ccagttcatt
     1981 ttcatgcgcc tgcaaaatgg cctgcaaacg tccgtcaaca ccacgttggg tgacttttac
     2041 acccgctaaa ggccagcgga tttcatcgat gtccagcgaa tcgatgactc ttccctttga
     2101 acgaagtaac gcgcctggct caaaggtcag cgttgggtca gacagacttc cacttagctg
     2161 cgcaggtaaa cgggcatata aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag
     2221 aggcagttga ctgttatcca tgcttaattt tcctgggcca aaattaagca ccgcgttgcc
     2281 cttacccgct tgcccctggg tcagcacatt cagtcgtccg ctgaccagag cgttctcaag
     2341 ccctgcctgc cagttgtcga ctttgacacc cagtcggcca ctcaaaggaa aaccagcata
     2401 cagccagctc cagcgaccat cgcttacggt caattgttga cgagtaattt gccacggcaa
     2461 atcgagcaac ggatcgccgt tatcccgtgc cagcacgatc aattgcccgc tattttcctg
     2521 ccagtccagc tcggcatcca ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc
     2581 atgcccactt acaggaagtc catccggcac gagcggcata gtaaattccc ccaccagttt
     2641 aaccggcggc tgattttcaa acgcgacgac atccagttcg ctgactgtaa gttgttgccc
     2701 tttcagctgg ccttgaaatt taactttttc gccctgataa cgcagttgct ggatatcaga
     2761 ggttaatgcg agagagagtt ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc
     2821 gatattgatc caggtgttag gcagcatgga ctgccactgc gcgagggttt ttggagcagc
     2881 tggcgattgc tccgtctgcg gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc
     2941 gacgttcaat aaccagcggc tgggatgtga aaggctggcg ttggtgatat gcgcaagctg
     3001 acaatctccc accagataac ggagatcggg aatgattaaa cctttacgcg taatgcgtgg
     3061 gctttcatct aatgcaatac gtgtcccgag cggtagccag atgtccgcca gcgtgggaac
     3121 ccacagcccg agcgtcatca gcagcgtcaa cggcacaaga ataatcagta ataacagcgc
     3181 gagaacggct ttatatttac ccagcatggg tagttaatat cctgatttag cgaaaaatta
     3241 agcattcaat acgggtattg tggcatgttt aaccgttcag ttgaaggttg cgcctacact
     3301 aagcatagtt gttgatgaat ttttcaatat cgccatggct ttcaattata tttgaaattt
     3361 tgtaaaatat ttttagtagc ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa
     3421 ctcgccgttt atagcacaaa acagtacgac aagaagtacc tgcaacaggt gaacgagtcc
     3481 tttggctttg agctggaatt ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc
     3541 aatggctgcg aagcggtatg tattttcgta aacgatgacg gcagccaccc ggtgctggaa
     3601 gagctgaaaa agcacggcgt taaatatatc gccctgcgct gtgccggttt caataacgtc
     3661 gaccttgatg cggcaaaaga actggggctg aaagtagtcc gtgttccagc ctatgatcca
     3721 gaggccgttg ctgaacacgc catcggtatg atgatgacgc tgaaccgccg tattcaccgc
     3781 gcgtatcagc gtacccgtga cgctaacttc tctctggaag gtctgaccgg ctttactatg
     3841 tatggcaaaa cggcaggcgt tatcggtacc ggtaaaatcg gtgtggcgat gctgcgcatt
     3901 ctgaaaggtt ttggtatgcg tctgctggcg ttcgatccgt atccaagtgc agcggcgctg
     3961 gaactcggtg tggagtatgt cgatctgcca accctgttct ctgaatcaga cgttatctct
     4021 ctgcactgcc cgctgacacc ggaaaactac catctgttga acgaagccgc cttcgatcag
     4081 atgaaaaatg gcgtgatgat cgtcaatacc agtcgcggtg cattgattga ttctcaggcg
     4141 gcaattgaag cgctgaaaaa tcagaaaatt ggttcgttgg gtatggacgt ctatgagaac
     4201 gaacgcgatc tgttctttga agataaatcc aacgacgtga tccaggatga cgtattccgt
     4261 cgcctgtctg cctgccacaa cgtgctgttt accgggcacc aggcattcct gacagcagaa
     4321 gctctgacca gtatttctca gactacgctg caaaacttaa gcaatctgga aaaaggtgaa
     4381 acctgcccga acgaactggt ttaatcttgc agctcccctg caatccaggg gagctgattc
     4441 agataatccc caatgacctt tcattctcta ttcttaaaat agtcctgagt cagaaactgt
     4501 aattgagaac cacaatgaag aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg
     4561 gatgtgtaag taatgacaaa attgctgtta cgccagaaca gctacagcat catcgctttg
     4621 tgctggaaag cgtaaacggt aagcccgtga ccagcgataa aaatccgcca gaaatcagct
     4681 ttggtgaaaa aatgatgatt tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac
     4741 tgtctaatgg tgaactgaca gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc
     4801 cgcagcttaa tgaactcgat aa
//
LOCUS       DAJTRD010000190         4773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTRD010000190 DAJTRD010000000
VERSION     DAJTRD010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.191"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(267..1124)
                     /gene="sitD"
                     /locus_tag="OO315_004036"
     CDS             complement(267..1124)
                     /gene="sitD"
                     /locus_tag="OO315_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5892725.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="OO315_004037"
     CDS             complement(1121..1978)
                     /gene="sitC"
                     /locus_tag="OO315_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5892726.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="OO315_004038"
     CDS             complement(1975..2802)
                     /gene="sitB"
                     /locus_tag="OO315_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5892727.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="OO315_004039"
     CDS             complement(2802..3716)
                     /gene="sitA"
                     /locus_tag="OO315_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5892728.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4148..>4773)
                     /locus_tag="OO315_004040"
     CDS             complement(4148..>4773)
                     /locus_tag="OO315_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5892729.1"
                     /translation="TEFNALMESEKGQSQNLWKFAVYSGLRHGELAALAWEDVDFEKG
                     IVNVRRNLTILDMFGPPKTNAGIRTVALLQPALEALKEQYKLTGHHRKSEITFYHREY
                     GRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHTFACW
                     LLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1264 a         1168 c         1126 g         1215 t
ORIGIN      
        1 gagctgaagg aagagctgaa tagtctgaag gatttgaatt cggttaggaa ttttatcgtg
       61 gaataacaga agctgcggca cgtcgtatgc aggaacgtgc cgcggttggc tggtaaactt
      121 tcgatagtgc gagtattgaa tgatttctag gcgttatcga ttttacgtat tttttgcatg
      181 atagaattcg tacctcctcc caacgacctt ccatggcttt ccggacttct gtaggctggc
      241 ggacgatgct gtcctcggat gttgtgctaa ttgggtgata acagatcctg tgtatgggcg
      301 tttccctttg tgcgtgcgtt gatgctggta acggtaaacg tcatgatgaa cacgaccgcg
      361 aataagacga caatcgtcgg agctggtgcg ctgtcgataa aaaaggacag ccagacacca
      421 gtcattgaca ctattaccga tatgccagtc gccagtagga gcgcaatatg aaagcgttgt
      481 gtcaggagta ctgcaatggc gccaggcgca atgagcagag aaatagagag aatgatacct
      541 actgctttca gcgtcgccac aatggtcagg gaaaccatgc acagcagccc ataatgcagc
      601 catctcgtat gcaaaccgct tacctgcgcc tgttgataat caaagctgaa cagcaaaaaa
      661 tcccgccatt ttacgctaat aacaagtgta accagcccgg caataatcac cgtctggatt
      721 atgtcgccga tggttatccc gagcatgtcg ccgaaaagaa tatggtcaag atgtacgtct
      781 ggtttaattg caatatacaa gatcagacct gcggcaaaca tgccagaaaa aacaatcccc
      841 ataactgtat cttgcttgat tcggctgttg tctttcaggt atcccgtcgc caccgcgcag
      901 aatactccgg cgacgaatgc ccctgtagcc agaggtaacc ccagaatcca ggtaagcacg
      961 atgccaggaa aaaccgcatg actcatcgcg tcacccatca gtgcccaacc tttcaggacc
     1021 agaaagaccg acagcagcgc acagggtatg gaaactacca gtgaaatcag cagtgcgtga
     1081 ctcataaagg tgaactgcag tggctccagt aataatgcca tcatgagcga tcctccagcg
     1141 ctttatgcgc acgccgacga tttgccaaaa gtccatgggt gggcgcaaat acaaatgcta
     1201 aaagaaacag cagtgtctgc gccaccacga tgattccacc tgtcgcgccg tccagaaagt
     1261 aactgaccca cgcgccgagg aaactggtta cactgccaat agttacagca attatgagca
     1321 ggcggggaaa acgatcggtt aacaaccatg ctgttgcacc gggagtcacc accagacaga
     1381 tcaccagaaa ggcaccaacc gtttgcagcg ccgcaacggt cgaaaccgac agcagggtga
     1441 agaagaggat ttttaatctg cccggatgta atccaatagc acgggcgtga ttctcgtcga
     1501 aaaaggtcac cataagatct ttccatttga aaaaaagcac gatgatcgag agaatgccaa
     1561 tgatagtgag ttgcaggata tccgccggat cgattgccag aatattgccc aggacgatgg
     1621 tctgaatgtt cacggatgtc gggttcaatg acaccataaa cagccccagg ccaaaaaagg
     1681 acgagaagat caggccgatg atggcatctt cttttaagcg ggtgcgttgg ttaagaaaca
     1741 gcatgctgcc cgctgccagt ccgccggaaa agaaggctcc cagagaaaac ggaagcccca
     1801 gcatatatgc ccccgcaaca cccgggacaa tagagtgcga cagcgcatcg ccaatcagcg
     1861 accagccttt tagcatcaga tagcatgaca ggaaagcgca gagaccgccg accattgccg
     1921 agacccacat cgcattgagc atatattcat agctgaaggg ttccagtagc acgttcatct
     1981 tgcttccctc tgcaccgctg acggtcgatg tgccacaaaa gggcgttcat cgtcagtaat
     2041 aatgctttct tccgagccgt taagtgtgac atggcgcagt acgccgctaa aagccagctc
     2101 caggttctcg gctgtaaaag tggtgtctgt cggcccgctt gccaggacgg tgcctttgac
     2161 catgaccgta taatcgcaaa atgtcgtgac tgagccgaga ttgtgcgttg agacaagcat
     2221 cgttttgcct tccgcgcgta actccctcaa caggctgatg attttcgctt cggttttcac
     2281 atcaacgccg gtaaaaggtt catccagcag gatcacatca ccctgctgtg caattgctct
     2341 cgccagaaag acgcgtttct tttgtccacc ggaaagctcg ccgatttgac gatggcgaaa
     2401 atccaccata tcgacacgtt ccagcgcatc ggtgacaatc tgacgatccc tctttttggc
     2461 tatacgcaac atacccatat gcccatagcg gcccatcatc accacatctt ccaccagtac
     2521 aggaaatgac cagtcaacct cttctgactg agggacgtag gcaaccaggt ttttctgtag
     2581 cgcctgtcgc gtgggaatac ccagaacaga tatctttccg ctggtcagac gcacaaatcc
     2641 cataatcgct ttaaacaacg tcgattttcc ggaaccgttt accccaacca gagcggcaat
     2701 agatccactt ggtactgtga aggatgcatc gcgcagtgct gtgtgcccgt tacgccaggt
     2761 gacagtaaca tcattaacga caatgcctgc agattgcatc attatttctc ccttttcccg
     2821 gctttaattc cctgtaccag cgtactggta gtaaccttaa gaaggtcgat atacgtaggt
     2881 accgggccgt tttctgtgct cagggaatcg acatagagca caccgccgta gtgtgcgccg
     2941 gtttcacgcg caacctgacg cgctggttta tcggaaatcg tactctcgct aaagactgcc
     3001 gggatatgat ttttcttaac tatatcaaca accttacgta cctgctgcgg tgttccttgt
     3061 tgatcggcat taatcggcca cagataaagc tcttttagcc ccaaatcgcg tgcgagataa
     3121 gaaaaagccc cttcactggt gaccatccat cgctgattct cagggagttc cgtaatctgc
     3181 ttacgcaggg gggcaagggt ttgggtaatc ttggctttat aagtatcggc attacgttgg
     3241 taggtttgtg catttgccgg atcgtatttt atcaacgcat cacgaatatt atcgacgtaa
     3301 atcagagcat tatctggcga catccatgca tggggattag gtttgccctc atagggtcct
     3361 tcggtgatcc ctactggcgt cacacccgaa gagacaatta cttctggaac cccattgaga
     3421 tgctggtaaa agcgttggaa ccacaattcc agattcatac cattggcgag aatcagttgt
     3481 gccccctgcg cacgtttaat atcgccaggg gtaggctgat actcatgaat ttctgcacca
     3541 ggcttggtta tggatgagac ttctgcagca tctccagcca cgtttttggc catatccgcg
     3601 atgatggtaa atgttgtaat gaccttgaat ttttcagttg cacttgcctg aaatgcgatc
     3661 gagcaggtga gggcgaggca ccccaagagc atggttactt tttttattga gtgcataatt
     3721 tagtacctat agtgagcaaa attagaacaa acatagcaac ggctatgttt tatagcataa
     3781 cctatacaaa atgaaaataa ttatcattaa cagctttgag gcctaaaaca gggattaggt
     3841 tgtttcctgc tggcgacatg atattgagga aaaaagtaga gcagctatta ggctttgaaa
     3901 tgactttatg aggaaaattg ttgatggcaa aaatggggca aaaaagagtg ggttatcgta
     3961 gcttattgtt gtcgctgata atgtttaaca tattgaaaaa taaataaaat acttatgagt
     4021 cagatagttg tgatttttgc ccttacttgt tcaggttgta ttgttctttc tttgtaattt
     4081 gttgattttc ttgcattatt tcagttctct ggtactaaat ggggcaaatt gggggcaaac
     4141 tttgcaacta cgataaccgc gcattcaaca tggctatctg ttcgtcgttc atgtcatcaa
     4201 tccacatacc gtaaatttca tacaccattt gcgcagtttc atgccccatc tggctggcta
     4261 taaatgccgg gttcgctcct gccgtcaaca gccagcaggc aaaagtatgt cgcgtatggt
     4321 acggattacg gcggcgaata ccagcacgtt ttactgctgc attccatctt gtacccaaac
     4381 tgcttaccga gtaataaggt ttctgttttt cgttacacac cctgggcatg aaaacaaaat
     4441 gcagtttttg tttttcagtt ctgccgtact cccgatgata aaaagtgatt tcgcttttgc
     4501 gatgatgccc ggtcagtttg tattgctcct tcagtgcttc aagagccggc tgcagtaatg
     4561 ctaccgtccg gatcccggca tttgtttttg ggggaccgaa catatcaagt atcgtcaggt
     4621 ttcttctgac attcacaatt cccttctcga aatccacatc ctcccacgcc agagctgcca
     4681 gttccccgtg acgaagcccg gagtaaacgg caaatttcca caagttttgg ctctgtcctt
     4741 tttcactttc cattaatgca ttgaattctg ttt
//
LOCUS       DAJTRD010000191         4614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTRD010000191 DAJTRD010000000
VERSION     DAJTRD010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.192"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..372
                     /gene="bglB"
                     /locus_tag="OO315_004041"
     CDS             <1..372
                     /gene="bglB"
                     /locus_tag="OO315_004041"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5892730.1"
                     /translation="LRVLLNTLWDRYQKPLFIVENGLGVKDSVEADGSIQDDYRIAYL
                     NDHLVQVNEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRT
                     RKKSFGWYAEVIKTRGLSLKK"
     gene            458..2074
                     /gene="bglH"
                     /locus_tag="OO315_004042"
     CDS             458..2074
                     /gene="bglH"
                     /locus_tag="OO315_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HCR5892731.1"
                     /translation="MFRQNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMIDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTHTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIARNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNNIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRLEIRFYATYI
                     KALENELDGFTFEDNKDAQFAVGAQAEIWW"
     gene            2101..3276
                     /locus_tag="OO315_004043"
     CDS             2101..3276
                     /locus_tag="OO315_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCR5892732.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFSVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKFKIRH"
     gene            3278..4000
                     /locus_tag="OO315_004044"
     CDS             3278..4000
                     /locus_tag="OO315_004044"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5892733.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELAFG"
     gene            complement(4062..>4614)
                     /locus_tag="OO315_004045"
     CDS             complement(4062..>4614)
                     /locus_tag="OO315_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HCR5892734.1"
                     /translation="YHPKPLETGAFEQDKTVECDCCEQQTSVYYSGPFYCVDEVEHLC
                     PLCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGY
                     HGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKG
                     GDCQGYLFRCLHCGKLRLWGDFS"
BASE COUNT         1279 a         1021 c         1126 g         1188 t
ORIGIN      
        1 ttacgggttc tgttaaatac gctttgggat cgttatcaaa aaccgttatt tattgtcgag
       61 aatggattag gcgtaaaaga cagcgttgaa gcggatggtt cgatacagga cgattatcga
      121 attgcctatt taaacgatca cctggtacag gtaaatgaag cgattgccga tggtgtggat
      181 attatggggt acaccagttg ggggccaatt gatttagtca gtgcatctca ttcacaaatg
      241 tctaagcgct acggctttat ttatgtggat cgtgatgata atggcgaagg aagcctcaca
      301 agaacgcgta agaaaagctt cggatggtat gcagaagtga tcaaaacgcg ggggctgtca
      361 ttaaaaaaat aaccaataaa gcaccttaat tatcgtcgca ttcagaacag tctggatgcg
      421 atacgttaat tctttctttg caccataaag ggatattatg tttagacaaa atcttattac
      481 ctctgccatc ttattaatgg caccgttagc cttttctgca caatcattgg ctgaatcatt
      541 aacggtggaa caacgccttg agttattaga aaaagcgtta agagaaacgc aaagcgaact
      601 caaaaagtat aaagatgaag agaagaaaaa atatacgcca gcgacggtga atcgtagcgt
      661 aagtacgaat gatcaagggt atgccgccaa tccgttcccg accagtagtg ccgcaaaacc
      721 tgatgctgta ctggtcaaaa atgaagagaa aaatgccagt gagacaggct cgatttattc
      781 ttccatgact ctgaaagatt tcagtaagtt tgtgaaagat gaaattggct ttagttacaa
      841 cggctattac cgttctggtt gggggaccgc ctctcatggt tcacctaaat catgggcgat
      901 tggttctctg ggccgctttg gtaacgaata ctccggctgg tttgatttgc agttaaaaca
      961 acgtgtctac aacgaaaacg gcaaacgggt tgatgccgtt gtgatgatag atggtaacgt
     1021 tggtcagcag tactctaccg gctggtttgg cgataatgcc ggtggcgaga actttatgca
     1081 gttctccgat atgtacgtta ccaccaaagg tttcctgccc tttgcgccag aggctgattt
     1141 ctgggtgggt aaacacggtg cgccgaaaat tgaaatccag atgctggact ggaaaacgca
     1201 gcgtactgat gctgcagcgg gtgtaggtct ggaaaactgg aaagtcggtc cgggtaaaat
     1261 tgatatcgcg ctggttcgcg aagatgtcga tgattacgat cgcagcctgc aaaacaaaca
     1321 gcagattaat acccatacta ttgatttacg ctataaagat atcccgttat gggataaagc
     1381 gaccttaatg gtgagtggtc gttatgtcac ggcaaacgaa agcgcatcgg aaaaagataa
     1441 tcaggataat aacgggtatt atgactggaa agatacctgg atgttcggca catctttaac
     1501 gcagaaattt gataaaggtg gcttcaacga attctccttc ctggttgcga ataactctat
     1561 cgccaggaac tttggccgtt atgctggcgc aagtccattt accaccttta atggtcgtta
     1621 ttatggtgat cacaccggcg gaacagcagt tcgtctgacc tcgcagggcg aagcctatat
     1681 cggcgatcat tttattgtgg ctaacgcgat tgtttactcc ttcggtaaca atatttatag
     1741 ctacgaaaca ggcgcacact ctgatttcga atctattcgt gcggttgttc gcccggccta
     1801 tatttgggac caatataacc agacaggtgt tgaactgggc tatttcaccc agcaaaacaa
     1861 agatgcgaat agtaataaat ttaatgagtc tggttataaa accacgctct tccatacctt
     1921 taaagtcaat accagtatgt tgacctcgcg tctggaaatt cgtttctacg ccacgtatat
     1981 caaagccctg gaaaacgaac tggatggctt caccttcgaa gacaataaag acgcccagtt
     2041 tgctgtcggt gcccaggctg aaatctggtg gtaatggaac ttaatgaaag gaatattacg
     2101 atgaatataa aaattgctgc tttaacgctg gctattgcca gcggtatttc tgctcagtgg
     2161 gccatagcag cggatatgcc agccagcccg gcacccacta tcccggtgaa acagtatgtg
     2221 actcaggtca atgccgataa cagcgtgacc tttcgctact ttgcccctgg ggcaaaaaat
     2281 gtctctgtag tggtgggtgt tccggttccg gacaatattc acccgatgac caaagacgaa
     2341 gcaggagtct ggtcgtggcg cacacctatc ctgaaaggca acctgtacga gtattttttc
     2401 aatgttgatg gtgtacgcag cattgataca ggcaccgcaa tgactaagcc tcagcgccag
     2461 gttaactcca gtatgattct ggtgccaggc agttatctgg atacgcgttc tgttgcgcat
     2521 ggtgatttga tcgccataac ttaccactcc aacgcattgc aatctgaacg tcagatgtat
     2581 gtctggaccc cgccaggata caccggcatg ggcgagcctt tgccagtgct ctatttctat
     2641 cacggttttg gtgataccgg acgttccgct atcgatcagg ggcgtatccc gcaaatcatg
     2701 gataacctgc tggctgaagg gaaaattaaa ccgatgctgg tggtgatccc ggataccgaa
     2761 accgatgcga agggcattat tcccgaagat ttcgtgcctc aggaaagacg taaagtcttt
     2821 tatccgctga atgctaaagc ggcagatcgc gaactgatga acgatattat cccgctgatt
     2881 agcaagcgtt ttagtgtccg taaagatgcc gatggtcgcg cgctggcagg gctttcacaa
     2941 ggcgggtacc aggcgctggt ttccggaatg aatcatctgg aaagctttgg ctggctggcc
     3001 acattcagtg gtgttaccac gacaaccgta ccggatgaag gtgtcgcggc ccggctgaac
     3061 gatccggcag ctattaacca gcaactacgt aattttactg tggtcgttgg tgataaagat
     3121 gtcgtaaccg gcaaggatat cgccgggctg aaaactgagc ttgagcagaa aaaaattaag
     3181 tttgattacc aggaatatcc cggcctgaac catgaaatgg atgtctggcg gcctgcctat
     3241 gcagccttcg tacagaaatt taaaataagg cattaagatg aaattaatca ttaccgaaga
     3301 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     3361 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     3421 caccctggtt aaaggtaagc cttggtacga taactgctat ttctataatt tcgatgaaat
     3481 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     3541 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     3601 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctgg gattaggtgc
     3661 agatggtcat ttttgtggca atttgccgaa tacgacccat ttccatgagc aaaccgtgga
     3721 gttcccgatc cagggagaga tggttgatat tgtggcgcat ggtgagttgg gcggcgactt
     3781 ttcgttagtg cctgacagct acgtgacgat ggggccgaaa agcatcatgg cggcgaaaaa
     3841 cttgctgata attgtcagtg gcgcaggtaa agcgcaggca ctgaaaaatg tgctgcaagg
     3901 tccggttacc gaagatgttc cagcttccgt gttgcagctg catccttcgc tgatggtaat
     3961 cgctgataaa gccgctgccg cagaattagc gttcggttaa gctgaagaaa taaggccgga
     4021 tgtatgaatt acgctccggc ctgttctcat tatttaaata actacgaaaa atcaccccac
     4081 agtctcagct tgccgcagtg gagacagcgg aagagataac cctgacaatc accacccttt
     4141 tgcaggcatt tagctagatc agaatttcta ataccgaaat tctcacaatc ttcttcaagg
     4201 ttggcaaatt catcgaggcg atcttttata tcattccagc ccacatagcc gataaaagca
     4261 cagaaatcgc cacaatgcgc aagccagaat tcctgttgcc atccatgata acctggcgtg
     4321 cgttcaacca actctttcag catttcatca ggatatgtgt tcttaatacc ggcaaattcg
     4381 tcctcttcat catactcaaa ttcaacacct tctatgctgg catcatcctg aaaactacct
     4441 gcaaactttt ctgcagcaga accgtccgca atacacaacg gacagagatg ttcaacttca
     4501 tcaacgcaat aaaagggacc cgagtaataa actgacgtct gttgttcaca gcaatcgcac
     4561 tctacggttt tatcctgttc aaatgcgcct gtttccagtg gcttgggatg atat
//
LOCUS       DAJTRD010000192         4602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTRD010000192 DAJTRD010000000
VERSION     DAJTRD010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.193"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO315_004046"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO315_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5892735.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO315_004047"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO315_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5892736.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO315_004048"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO315_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5892737.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..>4602)
                     /locus_tag="OO315_004049"
     CDS             complement(3929..>4602)
                     /locus_tag="OO315_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HCR5892738.1"
                     /translation="IFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNV
                     KVQLEQAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAM
                     LGLATAVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQ
                     EIAFDMSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGAT
                     ASRSA"
BASE COUNT         1081 a         1296 c         1109 g         1116 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat cc
//
LOCUS       DAJTRD010000193         4561 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTRD010000193 DAJTRD010000000
VERSION     DAJTRD010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4561
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.194"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..545)
                     /locus_tag="OO315_004050"
     CDS             complement(<1..545)
                     /locus_tag="OO315_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892739.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2196)
                     /locus_tag="OO315_004051"
                     /pseudo
     CDS             complement(1207..2196)
                     /locus_tag="OO315_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2280..2771)
                     /locus_tag="OO315_004052"
     CDS             complement(2280..2771)
                     /locus_tag="OO315_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892740.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAERIAKELASQKE
                     RTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(3069..3815)
                     /locus_tag="OO315_004053"
     CDS             complement(3069..3815)
                     /locus_tag="OO315_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5892741.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         1148 a         1080 c         1135 g         1198 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgactac tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa cagtcttagc taactggagg gcatagacct gagggtcggc
     2101 gttagtcggc agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc
     2161 aatgccaaac tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa
     2221 tacttgaagt ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct
     2281 tacaagctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag
     2341 cgaccagcgg aatgagtagc gctgcggagg ttttcaccat aggtccggcg ttcagctttg
     2401 acctgttcgc ggcgctgtag ctctttttgc cattcgtctt cggtcatgaa catttcgcgg
     2461 gtacgctctt tctgagacgc tagctcttta gcgatacgct cagcttcagc tcgtttgttc
     2521 atctggcggc gttggtaggc ggtcatcagg tcattactga agaagttacc aatggtcacg
     2581 ccagcttcag ccagagcttc cagtaactcc atcgcctcgc ggctggtgga cggtaagcct
     2641 gacaggtagc ccggaatgag ggtcttgata gcgagcgaca acggatagga ctgaccgtcc
     2701 agactcacag tgttttcata aaggttgatg tcggtgactg acagcttcca gttgttgttg
     2761 ttgaacatca taggtaattc tcctcttgag agttgagact atcggtatcg caggcacagt
     2821 aaggcctacg ggtctccccg catcggtcat ctcaaaaggt gtttaaatta ggtattgagt
     2881 aggtgagcta ctaacggtta agcggcagtt tcatagcgac cagttgggct attagctcgt
     2941 ctcgggattg gtgctggcgg gtctcatctt ccgggtctag accaagctct ctgaggagga
     3001 cttcagtccg gtagccacgg taacggagag tcatgcgccc ctcaggtgtg cgcgggtcaa
     3061 tcatcatctt agattccctc caccttaccg gattgtgcca gcttcaggag gtgctcttgc
     3121 ttctgctggt cggtgccagt gaatgcaata aggatgcctc gcatcttgtc aaccatctca
     3181 ggcttaccga agacctccag ccccttagcg ttgaaccgga agttataccc acctgctgag
     3241 aacttctggt ctgacggctt aatgatacga aagcagtcgg aatacttcgg atttacagac
     3301 cactcgttac tgattgggtt cttacgaaag aggccacgat gctggagaag tgcggtcatt
     3361 agcttctgag cgggcagacc ataggttttg cagtagtcgc cccaagtcac gcctcggcgg
     3421 ttagcggact gaatggctgc tcgcttcttc tggtcttcca gttgaaggac tcgacgctct
     3481 aacgtctgaa tatggtcgta acacttggcg cggaggtgaa cggaatagcc agcgaccaga
     3541 atgaaagtat gcttacggtc gagacgatac attgttcggc tctcaccttt cgcgtccttg
     3601 tatacttcct cgccaacgag cccaaatttg gacccgttaa gctcgctgct caaagttgag
     3661 cagtgctcag ttttggcccc gttaagctct tcgaccatat tacgaatatc tcgtaaaacg
     3721 tgtttatgtt cttttcctgt cagctcagca atttcaactg aggtcatgga cacaagctgg
     3781 tcgaggttaa tgatgctgat ttcttgttgg gacatttacg tgtactcctt ttgtttgcac
     3841 cctgaagtcg caccccactg acctactcaa gcccccacgt aaagggagac cagcaggcaa
     3901 caggacgcaa acagaatgac ttgaggtaat tggggttagt ggtctgcgat gaccatagag
     3961 atgaaggttc gacagcatgt ccgagcgtgt gtggtgatag acttaagtca cgagaccaaa
     4021 gaacacacag gctgaccaga ggctgtcttg ccttaggctg gtggctagct tgctagcgtg
     4081 gaccacggtt tctcgcttgc cgtctctcag gaggtcagag gcgagatatg gtccctatag
     4141 tgatagttta gtcaaacagg aatgaacttc ggagaacttg acgcgaaagt gttcagtact
     4201 tccagtaaga cccgaagcat ggagaccaca tcatagcgac cataatttta cttcgggttg
     4261 gcgggagtgg aggctggact taaagttctt cttattggaa ttgtgataac acaatccata
     4321 acaagataaa ctcatggtct cctccagcct gctggtcaag actcaaagct ccaacctcag
     4381 acaccactga tctaacctct ttgagtttcc gcctgtccac aaatggatgg cagacctcag
     4441 taatggtgtc ttggttcttc agttatccgc gcagcgatta tcacagagcc aacttaaagt
     4501 cggatgtggt ctactgctcc ccgcctgtca aaagggaaga tagcttacgt taaaaggaat
     4561 g
//
LOCUS       DAJTRD010000194         4558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTRD010000194 DAJTRD010000000
VERSION     DAJTRD010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.195"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..771
                     /gene="ag43"
                     /locus_tag="OO315_004054"
     CDS             <1..771
                     /gene="ag43"
                     /locus_tag="OO315_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5892742.1"
                     /translation="RTEVAGMSVTAGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGG
                     YLNLIHNASGLWADIVAQGTRHSMKASSDNNDFRVRGWGWLGSLETGLPFSITDNLML
                     EPQLQYTWQGLSLDDGQDNASYVKFGHGSAQHVRAGFRLGSHHDMNFGKGTSSRDTLR
                     GSAKHSVRELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSQNGTSLDLQAGL
                     EARVRENITLGVQASYAHSINGSSAEGYNSQATLNVTF"
     gene            892..3408
                     /locus_tag="OO315_004055"
     CDS             892..3408
                     /locus_tag="OO315_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892743.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            3484..3939
                     /locus_tag="OO315_004056"
     CDS             3484..3939
                     /locus_tag="OO315_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892744.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(4058..4269)
                     /locus_tag="OO315_004057"
                     /pseudo
     CDS             complement(4058..4269)
                     /locus_tag="OO315_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4350..>4558
                     /locus_tag="OO315_004058"
     CDS             4350..>4558
                     /locus_tag="OO315_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HCR5892745.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTR"
BASE COUNT         1090 a         1175 c         1229 g         1064 t
ORIGIN      
        1 agaacagagg ttgccggtat gtctgtgacc gcgggggtat atggtgctgc tggccattct
       61 tccgttgatg ttaaggatga tgacggctcc cgtgccggca cggtccggga tgatgccggc
      121 agcctgggcg gatacctgaa tctgatacac aacgcctccg gcctgtgggc tgacattgtg
      181 gcccagggaa cccgccacag catgaaagcc tcatcggaca ataacgactt ccgcgtccgg
      241 ggctggggct ggctgggttc gctggaaacc ggtctgccct tcagtatcac tgacaatctg
      301 atgctggagc cgcaactgca gtacacctgg cagggactct ccctggatga cggccaggat
      361 aacgccagtt atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc cggcttccgt
      421 ctgggcagcc accacgatat gaactttggt aaaggcacct catcccgtga caccctgcgc
      481 ggcagtgcca aacacagtgt gcgtgaactg ccggtgaact ggtgggtaca gccttctgtt
      541 atccgcacct tcagctcccg gggggacatg agcatgggta cagccgcagc cggcagtaac
      601 atgacgttct caccgtcaca gaatggtacg tcactggacc tgcaggccgg actggaagcc
      661 cgtgtccggg aaaatatcac cctgggcgtt caggccagtt atgcccacag catcaacggc
      721 agcagcgctg aaggttataa cagtcaggcc acactgaatg taaccttctg ataattctgc
      781 attgcctctc tgtggtcccg gtcatgatga ccgggacccg gacgggggaa aacgtttcag
      841 tgccacattc actggcattc acaataacat gatattcatc acggagtgac tatgttacag
      901 atagtcggcg cgctgattct gctgatcgca ggatttgcca ttcttcgcct tttgttcaga
      961 gcattaacca gcacagcgtc tgcgctggca gggttcatat tgctgtgtct gttcggtccg
     1021 gctttactgg ctggctatat cactgaacgc ataacccggt tattccatat tcgctggctg
     1081 gcaggcgtat ttctgacgat tgccggaatg atcatcagct tcatgtgggg acttgatggt
     1141 aaacatatcg cactggaggc tcatactttt gactctgtaa aatttattct gaccaccgct
     1201 ctcgccgctg gtctgctggc tcttcccgtg cagataagaa ccattcagca gaacgggctc
     1261 acacctgaag atatcagcaa ggaaattaac gggtattact gctgttttta tactgctttt
     1321 ttccttatgg cgtgttctgc atacgcacca ttgatcgcat tgcagttcga tatttcaccc
     1381 tcactgatgt ggtggggcgg gttgttgtac tggctggctg cattagtgac gctgctatgg
     1441 gcggccagcc agatccaggc gctgaaaaga ctgaccagtg ccatcagcca gacactggaa
     1501 gaacaaccgg tgctcaacag taaatcgtgg ctgagcagtt tgcaaaacga ttacagcctt
     1561 cctgaaacgc tgacggagcg catctggctg acactcattt cacaacggat ttcccgggga
     1621 gaactgaggg aatttgaact ggcagacgga aactggttac tgaacaatgc ctggtatgaa
     1681 agaaacatgg cagggtttaa cgaacagttg aaagagaacc tgtcattcac acctgatgaa
     1741 ctgaaaacgc tcttccggaa ccgcctgaat ttatcaccgg aagcgaatga cgattttctc
     1801 gatcgttgcc tggacggcgg tgactggtac cccttttcag aaggccgccg ttttgtatca
     1861 ttccaccacg tggatgagct tcgtatctgt gcctcctgcg ggctgacaga agtacatcat
     1921 gccccggaaa atcataagcc ggctccggaa tggtactgct cctctctttg tcacgaaaca
     1981 gaaacactgt gtcaggacat ttatgaacgt tcttacaccg gttttatttc cgatgcaacg
     2041 gcgaatggtc tgattctcat gaaactgccg gaaacctgga gtacaaatga gaaaatgttt
     2101 gcttccggag ggcagggaca tgggtttgcc gctgaacggg gaaaccatat tgtcgacaga
     2161 gtccgtctga aaaacgcacg gatcctcggt gataataatg ccagaaatgg agcagacaga
     2221 ctggtcagcg gaacagaaat ccagacgaaa tattgttcaa ctgcagcccg tagcgtcggt
     2281 gcggcattcg acggacaaaa cggacagtat cgttacatgg gaaatcatgg tcccatgcaa
     2341 ctggaagtcc cccgtgatca gtatgccggc gctgtggaaa ccatgaagaa taagatccgc
     2401 gaaggtaaag tacccggtgt aaccgatccc gcagaagcgt cccggctgat tcgtcgggga
     2461 catctgactt atacccaggc ccgtaatatc acccggttcg ggaccatcga atcggtcact
     2521 tatgatattg ccgaggggtc ggttgtcagt ctggcggccg gagggatcag ttttgccctg
     2581 acggcatcgg tcttctggct cagcaccggc gatcgcgatg ctgccctgca gacagctgct
     2641 gtccaggcag gaaaaacctt cacccgcaca ctggctgtct acgtcacaac ccagcaactt
     2701 caccggctca ctgttgttca gggtatgctg aagcatattg atttttcgac ggccagcccg
     2761 actgtccggc aggcgcttca gaaggggacc ggtgcaggaa atatcagtgc cctgaacaaa
     2821 gtgatgaagg gtacgctggt gacatctctg gcactggtag ctgtcacaac cagccctgac
     2881 atgatcaaaa tgttgcgggg acggatctcc ggtgcgcagt tcatcaggaa tcttgccgtg
     2941 gcatcttccg gtgtggcagg tggtgctgtc gggtcagtgg cgggcgggat attgttcagt
     3001 ccactgggac catttggtgc actgacaggg cgtgtggttg gcggtgttct ggggggaatg
     3061 attgcctccg ctgtatcagg aaaaattgcc ggagcgctgg ttgaagaaga tcgcgtcaaa
     3121 attctggcaa tgattcagga gcaggtgaca tggcttgccg gcagtttcct gctgaccgga
     3181 catgagattg aaaatctgaa cgcgaatctg gcccgtgtta tcgatcagaa tgctctggag
     3241 atcattttcg ccgccggtat acaacaacgg gccgcgacca atatgttaat caaaccactg
     3301 gtggtcagta tcatcaggca acgccccgtc atggaatatg atgcatccca tctcggcaag
     3361 atggttaacc ggctggaaga agcattcccc ccggagctcc cggcataacg tgtgttctgc
     3421 tccttttagg ttttatcacc tgatttgcca gtgtcatcgt catgactcag agaggtaaat
     3481 accatgattc acctgttcaa aacctgcatg attaccgcct tcattctggg gttaacgtgg
     3541 tctgccccac tccgggcaca ggatcaacgt tacatcagta tacgcaatac agatacgata
     3601 tggctcccgg gaaatatttg tgcttaccag ttccggctgg ataatggcgg aaacggtgaa
     3661 ggatttggcc ccctcaccat cactctgcaa ctcaaagaca aatatggtca gacgctggtg
     3721 accagaaaaa tggaaacgga agcttttggt gacagtaatg ccacgcgaac cacagacgca
     3781 tttctggaaa cggagtgcgt ggaaaatgtc gccacaaccg aaatcattaa agcaactgaa
     3841 gaaagtaacg gccatcgtgt cagtctgccg ttatcggttt tcgatcccca ggactaccat
     3901 ccactgctga ttaccgtttc cggaaaaaat gttaactgat ccctgaagtc agtttcatgc
     3961 ttcagcctct gccacaacgt atggcagagg ctatttatca ggagaaacca gatatgccgg
     4021 gttacacaca gagtatgtcc tggctgaagg atctttctca tacgggcagg cggttgctgt
     4081 cataaccgct taccgcaatg tgtttatcca ggatgacccg ggaatgcatt tccgtcgggt
     4141 tatccgcaac gcggaaggtc agcgacgctg acgcagccgg aactctgaag ctgatgccgg
     4201 aaaacagctt aatgcgtggc tcgccagtgg agggcttctc agacaataaa cttcattgat
     4261 ttcatccatc aggccgcgtc ttctccggga gacgcggctt ttttcatttg ttgcaccagt
     4321 aaatcttaac caccgataag gagcaaagta tgcaattagt cagtcgtttt ggttatgcga
     4381 accagatacg ccgtgaccgt ccgctgacac atgaagagct gatgcaccat gtgccgggga
     4441 ttttcgggga agagaaacac acgtcccgaa gccagaacta tacgtacatc cccaccatca
     4501 ccgtactgga aagtctgcag cgggaaggct tccagccctt tttcgcctgc cagacccg
//
LOCUS       DAJTRD010000195         4548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTRD010000195 DAJTRD010000000
VERSION     DAJTRD010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.196"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..1222
                     /locus_tag="OO315_004059"
     CDS             <1..1222
                     /locus_tag="OO315_004059"
                     /inference="COORDINATES: protein motif:HMM:NF027197.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5892746.1"
                     /translation="PYGIFQQRNVLASWLATGCGVALYLALTARTRSLTLICLLSLYP
                     LSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLIFVASLLCWCVGISN
                     YAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSFEATFPQALEDVGVT
                     NIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRLKMTNNNAHHQCEKW
                     FIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQVRIPKTVRLGILPV
                     IVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKYLTQAERLERDQHLL
                     IANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRRGDYLTAEKIRKRAA
                     RVFVNDERFTRGGN"
     gene            1222..1956
                     /locus_tag="OO315_004060"
     CDS             1222..1956
                     /locus_tag="OO315_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5892747.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2424..3185
                     /locus_tag="OO315_004061"
     CDS             2424..3185
                     /locus_tag="OO315_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5892748.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3229..4308
                     /locus_tag="OO315_004062"
     CDS             3229..4308
                     /locus_tag="OO315_004062"
                     /inference="COORDINATES: protein motif:HMM:NF025320.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005093492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5892749.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNGQIIFTRSVPPGPFALDNLPTLAVGGELDVTVRESNGEEQYFSVPF
                     QTPAIALHEGYFKYSVMGGNIKKKV"
     gene            4305..>4548
                     /locus_tag="OO315_004063"
     CDS             4305..>4548
                     /locus_tag="OO315_004063"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5892750.1"
                     /translation="MKSSPVSVGEITAMYGLPYGVTVYGGIQNATHFNAISTGIGISL
                     GLLGSLSTDITRSIANLYYGNKYRIRYSKSISDFGTQ"
BASE COUNT         1247 a          864 c         1120 g         1317 t
ORIGIN      
        1 tccgtatggc attttccagc agcgaaatgt tcttgcgagc tggctggcaa caggttgcgg
       61 cgtagcgttg tatctggctt taacggcgcg tactcgttct ctgacgttga tatgtttgtt
      121 gtcgttatac cctctgtcta cagctttagt ccttacacaa agccgtgtcg gccttcttgg
      181 tttattttgc gtcatcgtgc tggccgctct cgcggacaga ccccgctggc gcggacgtcc
      241 ccttagcggt ctgttgcgct tgatcttcgt cgcctccttg ctgtgctggt gtgtcggtat
      301 ctcaaactat gccatgccag gggagacaac ggctgacttt acgcattcat catcgaatga
      361 ccagcgtata cgtattctga aaggtgccat aagtcttatt caacagcgcc ccttaaccgg
      421 ttgggggtta ggcagctttg aagcgacctt cccgcaagcc ttagaggatg tgggcgtgac
      481 aaacatagag agtgatacgg ctacgcatcc ccataacgaa ctgctgttcg taacggctga
      541 aggtggcatc gtcgccttgt ttggtttttt gctgctatca ggcgtttggc tgtggccgtg
      601 tatatgccgc ctgaaaatga caaacaataa cgcgcaccat caatgtgaaa agtggtttat
      661 tgccttaatg gggttgcctt tagtcattca tatgatgacc gaatatcctc tctatacgtc
      721 tgttccccat ttgatgctgt tgctgctcct tttccgcatc ggaatgccgg aaaacgtgat
      781 gaatcaggta cgtataccta aaacagtccg gttgggtatt ctgcctgtca ttgtgctcat
      841 tatgaccgcc gcgctggtca tcctggtagc gggatttgat gcacagcggg aactgaccag
      901 agctgaagct gatatgaacg aaggatttat ccctcgcctt ccggaacata gctggaaata
      961 cctgacgcag gcagaacgtc ttgagcgcga tcagcatctg cttattgcga ataaaccggg
     1021 gttcgttcgc gatacggaaa gcatgtctct tttcactgtg tggggaaata aatggttggc
     1081 aatccacaat gatgcagacg taagcgctgc aatgatattc attgcgcaca ggcgcggtga
     1141 ttatctgaca gctgaaaaaa taagaaaacg cgcagctcgg gtatttgtaa atgacgaacg
     1201 ttttactcgc gggggaaact aatggttaac gatctgacgt ataagaaatt agaaagcaca
     1261 aacatcaact gtaatattct ctcgccagaa aataccacat ggacaaatgt cacggtgatt
     1321 ggcctggatc tttttgccat ttgcggcgtg tgcagtttgc tcaagcgtgt tgggggagtg
     1381 aaacttcagg ttttcacttc cttacccact gatatgaatg ttctgactga cagtagcatc
     1441 gtgatatggg taaaaatgcg tcacgatggt ttgcctgaat tagccggaca tattgtgaag
     1501 gtttgtcgtc gataccgtct gatgcggcaa ctcgttatta gcgatgctat tcctgaagga
     1561 atgacgaatg atatcgggcc tctttcccga gtatggctta ctcatgggag tgcaaaagtg
     1621 gatattttat tatcttcatt acggaaggtg ttaaatgctc attgtttgcc tggtcctttg
     1681 tttataaaac gcctgggaag agtgcaatgg agggttcttt tactccgcgc gaaggggatt
     1741 agcacacgta aaattgcaga aatctgtggt attggcctca agaccgttag cgcccatgag
     1801 tccgcaattc gagaacgatt aggcaccagg aataaaagtg agtacgtctg gttagtacgt
     1861 aatgctcaac ttatacaaca ggcgataccc gcgttgggaa gagatgcgtc aaggtatgaa
     1921 agatgtaaca atatgcgaac aaagcaaaaa gtataattga tgcgtcatag ataactctct
     1981 ttttcgatga cgaaatcgct gagcatgcgt ggtgaggtta gtaatagatg ggtgctatgt
     2041 tttgccacgg gtttgtagct gtgaaacctg gaaaaaaata agaattaaaa atatttaaag
     2101 agagttaata aggttctggt tgtaattgtc ttggtcaaaa aaaagatatt gtcaatgttg
     2161 ttttttaaat tttcaggttt tggttaaata tttgcttgca aatataggtg atttttttat
     2221 tgtaggcttt tgaaggctat tataactgtg cgctaaatta atgcgttgaa taaaagaaat
     2281 aacaaaatta agaaaagcca tatttacgat atgggcaaca taaatataat ctaaaaaaga
     2341 gtaaggcgtt gtaggttggt tccgcgttca ttaccttgtt agtgaacgcg gatttatcag
     2401 ttgtcgaaat ggtttttata ataatgaaaa attttaatgt actaaccaaa gtcgtatttt
     2461 gcttgtcgtt tttatcaagc gcatttccca cctatgctgc ggatgatgtc aaagtgactc
     2521 aaaacacttc agtttttaaa ctgaagctta gtgcaacgcg attaatctat gaacaagggt
     2581 cggatggcgc agcgataact gtgacaaatc cgcaggattg gccgatgctt gtgcagagta
     2641 aagtctttga agaagataaa gtaactgcag cgccctttat tgtcactccg ccactgttca
     2701 ggcttaatcc aggccagcaa aataaaatgt gggttgtgca gactgcgcca gctttacaag
     2761 acagggaaaa attacactgg ctatgcgcca ccggcattac gcctaaaaag agtgatgttt
     2821 gggccgagaa agataaagaa aaaattgctg aaaatgtgaa cctgaacatc caggttaacg
     2881 tacatacctg cataaaactt attaccaggc catctggtat tgaaggcaag atggttaatg
     2941 cgagtgagaa gttatcgtgg aaaaagcagg gtgataaaat tgtcgcggtc aatcagtcgc
     3001 cgttctacat caacctttct tctttacagg ttggcggtag caagattgag aaactagatt
     3061 atattgcgcc gttttcgcaa catgagtttg ttatgccaaa aactagcggc caggatatta
     3121 aatggtctgt gattactgat gaaggtggtg agagtcgttt gtttatatct catttgcaat
     3181 cataatgtat tacattttta ttgaatattt ttatgccatg catattccat gttttatcga
     3241 ctgtcattaa ttttgataat ggccttactt gcgggtcagt taagtgccca ggagtggtca
     3301 tttgattctt cacagttgga aggaaacgtt agtgcggaca ctgtggcaat gtttaatcag
     3361 ggagagcaac tccccggaaa ttatcgtgtg gaaatttatc ttaacggtga aaaagttgat
     3421 gttggggagt ttccctttca tcgtccggaa tcccctgaag aaaaagagct tgttccatgt
     3481 ttaaccgttg atgaccttat ccactatggc attaaaattg ataaaagcag cagtgatact
     3541 gacaataaaa agaatcagtg ttttaaatgg aactctattg aaggtttgaa agtaaattat
     3601 gattttgata gccagcgcgt acagataacc gttccacaac tgtatctgca agataaaaaa
     3661 agtagtttag caccagtgtc gttatggaat gagggtgtag ctgcattccg catggtgtat
     3721 cagacaaata ttgatatttc caaacaaaat gataatcaat ctaccacgcg taattctcgt
     3781 tatggcaggt tcactccagg gtttaatctt ggtgcctgga ggtttcgtag ttcagtgaca
     3841 tggagcaagg aacttggcca gtcagaaaga tggcaaaggg gttacatgtg gtttgagcga
     3901 ggcataaatg cgattaaaag ccgattaacg cttggagaat cttatacgtc gtctgaggtg
     3961 tttgacagca taccttttcg cggcgggatg ttagcgaccg acgatgcgat gactccgccg
     4021 gaggacagct actacacacc tgtggtgcat ggaatagcac aaagtgaggc gcaggttatt
     4081 attaaacaaa atgggcaaat tatttttacc cgttctgtgc cgccagggcc ttttgcgctg
     4141 gataatttac caacgctggc cgttggcggg gagcttgatg ttacagtgag agagagtaat
     4201 ggcgaggagc agtatttttc cgtgccattt caaacgccgg ccatcgctct gcatgaagga
     4261 tatttcaaat atagtgtgat gggggggaat ataaagaaga aggtataaaa agctcccctg
     4321 tctccgtggg cgaaatcacg gcgatgtacg ggcttcctta tggcgtcacg gtgtatggag
     4381 ggattcaaaa cgccactcat tttaatgcaa tatctacggg tattggcatt tctctggggc
     4441 tgttagggag tttatcgaca gatattacgc gttcgattgc gaatttatat tatgggaata
     4501 aatatcgtat tcgttatagt aaatccatta gtgattttgg tactcaat
//
LOCUS       DAJTRD010000196         4383 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTRD010000196 DAJTRD010000000
VERSION     DAJTRD010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.197"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..311
                     /locus_tag="OO315_004064"
     CDS             <1..311
                     /locus_tag="OO315_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5892751.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="OO315_004065"
     CDS             468..1217
                     /locus_tag="OO315_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5892752.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..1805
                     /gene="yieF"
                     /locus_tag="OO315_004066"
                     /gene_synonym="chrR"
     CDS             1239..1805
                     /gene="yieF"
                     /locus_tag="OO315_004066"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5892753.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO315_004067"
     CDS             complement(1859..3196)
                     /gene="adeP"
                     /locus_tag="OO315_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5892754.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            3361..4017
                     /gene="yieH"
                     /locus_tag="OO315_004068"
     CDS             3361..4017
                     /gene="yieH"
                     /locus_tag="OO315_004068"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5892755.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSAIEGAGALLSAITAPMC
                     VVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVENCI
                     LVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWDITA
                     "
     gene            4084..>4383
                     /locus_tag="OO315_004069"
     CDS             4084..>4383
                     /locus_tag="OO315_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892756.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAV"
BASE COUNT         1176 a         1108 c         1080 g         1019 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc ggaatataac tactcggtac
     1501 cgggtgggct gaaaaatgcc atcgactggc tttcccgcct gccggatcaa ccgctggccg
     1561 gtaaaccggt gttgattcag accagctcaa tgggcgtgat tggcggcgcg cgctgtcagt
     1621 atcacctgcg ccagattctg gttttcctcg atgcaatggt gatgaacaag ccggaattta
     1681 tgggcggcgt gattcagaac aaagttgatc cgcaaaccgg agaagtgatt gatcagggta
     1741 cgctggacca cctgaccggg caattgaccg catttggtga gtttattcag cgagttaaga
     1801 tctaaataaa aaacccgcca gtaatcatgc atggcgggtt tttaatgcgc tatcgatttt
     1861 aatgagcgtc gataaataca atcttcagga taaacagcag cgcaacgatg attacgcacg
     1921 ggctaaggtc acgcagacgc ccggtaccaa tcttcatcac gcagtaggag ataaagccca
     1981 gcgcaatacc ttcggtaatc gaaaagctga acggcatcat cacggcggta ataaacgccg
     2041 gaacagattc agtaagatcc tgccagttca cgcgagcaag acttgaggtc atcaacacgc
     2101 caacgtagat caacgcgcca gctgcagcgt agtctggcac cattcccgcc agcggcgaca
     2161 gaaagataac cagcaggaac agcagaccaa caaccactgc cgtcagaccg gtacgaccgc
     2221 caaccgatac gccggaagag gactcaatat aagccgtaac ggaagaagta ccgataaacg
     2281 aaccggtcac ggaagagata ctgtcgacat acagcgcctg cttcatgcgc gggaatttcc
     2341 ccttctcatc cgccagacct gctttatcgg tcacgccaat cagcgtaccg gaggagtcaa
     2401 acaagttgac caacatgaaa gagaaaatca cccctgccag cccgaggtta aacgacccgg
     2461 ctaaatctac atgacccaca actgtcatta cgctcggcgg cgcagaaacg atgccattgt
     2521 agtgcacatc acccagcatc cagcccagca gcgtcgtcac cacgatagaa accagcaccg
     2581 ctgcgtgaat gttgcgcgag gccagaatag caatgatgaa aaagccgagg atacccagaa
     2641 gtacgctgtg agaagtcaga ttaccgatgc tcaccagcgt ttccgagtta gcgacaatca
     2701 cacctgcgtt tttcagcccc atcatgccga taaacaggcc gataccgctg gtaataccca
     2761 cacgcagact taccggaatg ttagcaatca tccagtaacg aacgcggaaa atggtcaaaa
     2821 gaagcagtcc gacagcgccc cagaagattg cgcccatacc gatctgccac ggcagcccca
     2881 ttgcctggac aaccacaaaa gcgaaaaagg cgttaagccc catcgcagga gccagcgcca
     2941 ccggaaggtt tgcaaacaat cccatcataa tgctaccgaa agcagcaatc agacaggttg
     3001 ttacgaagac ggcactggtg tccatgccag caacgcccag aatttgtggg ttaacaaata
     3061 cgatgtagac catcgtcagg aaggtggtga acccggcaat cacttcagtt cgtgccgtag
     3121 taccatgttc gcgcagttta aaaacacgtt ccagcatcct ctgaccagaa gtctgggtgg
     3181 tatgttgatg actcattatc tatttccgaa caaggaggga aaatccgtcg ctatcgtata
     3241 ccaaaatgcg acaataggcg cgtttgtgag agactttttt attggattta cttatacggc
     3301 aacgattgcg ttgcgcaaat cggctctacg aaaacgttaa actgattaaa aaggaaaggc
     3361 atgtcccgga tagaagcggt atttttcgac tgcgacggta cgctggtcga cagtgaagtc
     3421 atttgctccc gcgcatatgt aacgatgttt caggaatttg gtattacgct cgatcctgaa
     3481 gaggtattca aacgtttcaa aggtgtaaaa ctgtacgaaa ttatcgatat tgtttccctt
     3541 gaacatggtg ttacgttagc gaaaacagaa gctgaacacg tttaccgtgc agaagtcgct
     3601 cggctgttcg attcagccat cgaaggggct ggagcgctcc tgtcagcgat cactgcgcca
     3661 atgtgtgtgg tatctaacgg cccaaataac aaaatgcagc attctatggg caagctgaat
     3721 atgttgcact acttcccgga taaactgttc agcggctacg atattcagcg ctggaagcca
     3781 gacccggcgt taatgttcca tgcggcaaaa gcgatgaatg taaatgtaga aaactgcatt
     3841 ctggttgatg actcagttgc cggtgcgcaa tctggtatcg acgcaggtat ggaagtgttc
     3901 tacttctgcg ccgacccgca caataagccg atcgttcacc cgaaagtcac cacctttacc
     3961 catctttcgc agttaccgga actgtggaaa gcgcgtggtt gggatattac ggcatagttc
     4021 ttcacactcc cttcacttac cccgcttaaa ttggcgctca aaggtaagta aagggagttt
     4081 gatatgtctg tttcacgtcg ggtaatacat cacggacttt attttgcggt tttaggaccg
     4141 ttaattggtg ttctgtttct tgtcctctac atattcttcg caaaagaacc gctggttctt
     4201 ttggtaataa tacaggtact tccattgttt ctcttattgt cgattactac gggagctatt
     4261 cctgcattgt taaccggcgt gatggtcgcc tgtctgccgg aaaagatcgg gtcacagaaa
     4321 aattatcgtt gtctggctgg tggcataggt ggcgtcgtta tcaccgagat ctattgtgca
     4381 gtt
//
LOCUS       DAJTRD010000197         4209 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTRD010000197 DAJTRD010000000
VERSION     DAJTRD010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4209
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.198"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO315_004070"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO315_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5892757.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO315_004071"
     tRNA            complement(324..399)
                     /locus_tag="OO315_004071"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO315_004072"
     tRNA            complement(406..480)
                     /locus_tag="OO315_004072"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO315_004073"
     tRNA            complement(597..681)
                     /locus_tag="OO315_004073"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO315_004074"
     tRNA            complement(690..765)
                     /locus_tag="OO315_004074"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO315_004075"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO315_004075"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5892758.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO315_004076"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO315_004076"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5892759.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO315_004077"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO315_004077"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5892760.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1046 c          937 g         1137 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggctt
//
LOCUS       DAJTRD010000198         4189 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTRD010000198 DAJTRD010000000
VERSION     DAJTRD010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.199"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            85..753
                     /locus_tag="OO315_004078"
     CDS             85..753
                     /locus_tag="OO315_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892761.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(996..1691)
                     /locus_tag="OO315_004079"
     CDS             complement(996..1691)
                     /locus_tag="OO315_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5892762.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1684..3111)
                     /locus_tag="OO315_004080"
     CDS             complement(1684..3111)
                     /locus_tag="OO315_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5892763.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3122..3841)
                     /locus_tag="OO315_004081"
     CDS             complement(3122..3841)
                     /locus_tag="OO315_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5892764.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1115 a         1084 c          866 g         1124 t
ORIGIN      
        1 tttgctgata tttaacccga acattcctga tacagactat taatacaaca ttatctgcga
       61 cagattttat tttctggata tattatgagt gaacaaatca aacaggatat cgatctgatt
      121 gagattttat ttcatctgaa gaaaaagatt cgtgttatcc tttttattat ggctatctgt
      181 atggcaatgg tgctgttgtt tctgtatatc aataaagaca atacaaaagt gatttacagc
      241 cttaaaataa accagacaac gccaggtata cttgttagct gtgatagcaa taataatttt
      301 gcctgtcaga ccacaatgac tgaagatgtt attcagcgaa ttactacatt ttttcagacc
      361 agcccagata tcaagaacag agaaataagg ctggaatggt caggagataa gagagattta
      421 ccaactgctg aagaggaaat atctcgcgtg caggcgtcta ttatcaaatg gtatgcgtca
      481 gaataccata atggcaggca agttctcgat gagatacaaa cgccttcagc aattaacagt
      541 gagctttata caaaaatgat atacctgacc aggaactggt cattgtatcc gaacggtgat
      601 ggctgtgtaa ctataagctc accagaaata aaaaataaat accctgctgc catttgcctg
      661 gctctgggat tttttctaag cattgtaatt tctgtaatgt tttgccttgt caaaaaaatg
      721 gtagatgaat accaacaaaa ctctgggcag taataaacag cattctgata gagagtgcct
      781 gtataaagct gaagtgacaa tgaccgggaa gggaacccgt tttcaggaat ggatgcctgt
      841 ctttttcact gtaaatatcc tggcattact aatgcctgga tatttacaga agagcagcat
      901 aaccatgacg gaacggggtt aagtcaggtt gtccttaacg ctattttcaa cctcgtatgc
      961 cttcttcagg tttatgttca gatctcatat ctctctcagc aatcctcaat aatcagatac
     1021 accgctttca ccggaccatg aaccccgacg actttgataa gctcaatatc cgccgttgag
     1081 ctgggaccac tgatgatgtt aatacatgac ggcattcgtt caccggcctg cgctttctga
     1141 tgcaattttt ctgcgagttg cgctacacgc ggcaggattg tgcttttacg caggataaaa
     1201 agagaagatt ccgggagcag gctcaatgaa cgcccgcgct cggcggcgga aaaaagaacc
     1261 acgcctcccg attcggttaa accatattca gcatacacga cacccacttt agcctgctct
     1321 gcctgcgaga tattctcggc acctttcgcc ggatcccaaa caacggcatt gcattcctgc
     1381 tgcaaacgtt cactaatccc caattcctcc agcctcgtgt caccgctaat cacgaccgac
     1441 tgatctccca gctctttaca cagacgtatt gcagcttctg ccgccttcgc ctcgctggtc
     1501 agctcacagc gcgtcaacat aacatcgctg gcaaactgaa taaacgcgtc acagcgctgc
     1561 tgttggttaa gttgggtaag ccgctcgtta gcatagttgt taagcggcgc atcttctgct
     1621 tgcggttcaa gtcgcagcgg gcgacccagt gcctgagcaa cgttattcaa aaattcgctc
     1681 cgattatcca ttctttttct cctgcgcctg atgtttctta aaccaactac ggaaactctc
     1741 tccgtcagct tcaggaagat cgcgtgcttc catccagtcg ctaatcgcgc caaatttgag
     1801 tggtgttttg ccgccattga taaaccagct tgccgcatga gcgccggcca tcatcccgac
     1861 tttccacaat cctggatgac tattggcata agcgaacatt tttatcgccc gttgctctgc
     1921 ttttgcggtg atcccttttt cagccatcac ccgacgatga cgcaaaatca gttttgacag
     1981 cggaatacgc accggacaca cgctgtcaca agcagtgcat aaagagcagg cgtagggtaa
     2041 atctttaaaa tctttatagc cgccaagtag cggagaaatc accgcaccaa ttggccctgg
     2101 ataaatagag ccatatccat gaccgccaat atggcgatat gccggacaag tattcataca
     2161 agccccgcag cgaatacagc gcagcacatc ccgaaattca gaggccagca cctcagaacg
     2221 cccgttatcg acaataacca gatgaaactc ttcaggacca tcaacgtgcc ccgcttcgcg
     2281 cggccctgtc agccaggtgt tgtatcccgt caaacgtgca ccaacggcac tgcgcgccag
     2341 catggtgatc aatacatcta cctcggcaaa cgtgggggca atacgctcca ttcccatcac
     2401 tgcaatatgc gttttaggca gcgtggtaca cattcgcgca ttaccttcat tggtcaccag
     2461 gcataccgaa ccggtctctg ccaccgcgaa attacagccg gtaataccta tttcagcact
     2521 gaggaaatct tcacggattt tttgccggat gaataaggtc atcgcttcag gcgtttccgg
     2581 cccctcatag cccagacgtt cgtgtagcac tcgacggatc tgatggcgat ctttatgaat
     2641 tgccgggacc acaacatgag atggcggatc ttgatccagc tggagaatat attcacccag
     2701 atcggtttca atcacctgaa tgccagcatc ctgcaacaca tgattgacac caatctcttc
     2761 ggtcaccatc gatttagatt tcaccacctt ccgggcattt ttgcgttggg caacctgtaa
     2821 aatgtagcgg gtagcgtctt ctttggtttt tgcaaaatag acgtgaccgc cgttttgcgt
     2881 cactttttct gagagctggt acagataagc gtcgagatta ctcagaacat gatcacgtat
     2941 ctgggcggcc cgatcgcgcc actcctccca gtgccccaat tcatcgacca ttttttgccg
     3001 atttgcccca atacgctgct gcgcgcttgc caccgctttg cgcatgatcg gatcttcaat
     3061 ttgctgacgg atgcgtgtct taaaatctgt attactggtt ttgatcgaca tctttatatc
     3121 ctcagcggct catcaacact tcagcaatat gcatcacttt gactttctgc ccttcccgtt
     3181 gtaatcgccc actgatgttc agcaggcaac tcacgtcagc accaattaaa tactcagggc
     3241 ggacttccat caggtgcaca accttttctt tcaccatctc gccggatatt tcggccattt
     3301 tgaccgagaa cgtgccgcca aatccgcagc aggtatcctg ttcagcaaag gtcaacagct
     3361 ccagtccacg cacatttttc agcagcgtaa gtggctcgtc cttcactccc agcttacggg
     3421 ccaggctaca agatgggtga tacacagctc tcccttgcaa actggcacct acatcgacta
     3481 cccctaattt attaacaata aaagaggtga gatcctgcat acgcgcggca accttttcgg
     3541 cacgtgatgc ccattcaggt tcatccgcca gatacgtcgg gtaacttttt atggcatagg
     3601 tgcaagagcc agccggtgaa ataatgggat cgtcgttatc ctccagtgcg gcgatcagat
     3661 ttttcatccc tggaatcgct tctttgatat aaccgctatt gatcgcaggc tgaccgcagc
     3721 atccctgttt ctccgggaaa tttacgcgac agccgagttt ttccagtagc agcacggagt
     3781 ctcgtgccat tcttgatttc agggcgtcac caatacaggt gacaaagaaa ttgacattca
     3841 caactaatac tccattactt catgcccatt tatgcccgat gtttcccccc caccggggga
     3901 tcaatcatca ataaatatgg cgttcttttt taattggcta gcaacatacc acccctcatt
     3961 tgtatgacaa atgataatct ttcatccaaa cttcaaatgc aataactaat tttatttctt
     4021 tcggcatcgc tcacatttta tggggaaatc gacgatcatt tttggcataa gaaatccatt
     4081 taaaaatggc tacatccctt atacctattc agagctactt attcaggggt tattgagata
     4141 tacactgatg gtgtacaaaa aaatcaccga caataatcac gtatcaagt
//
LOCUS       DAJTRD010000199         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTRD010000199 DAJTRD010000000
VERSION     DAJTRD010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.200"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(348..995)
                     /gene="ychE"
                     /locus_tag="OO315_004082"
     CDS             complement(348..995)
                     /gene="ychE"
                     /locus_tag="OO315_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5892765.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1472..4147
                     /gene="adhE"
                     /locus_tag="OO315_004083"
                     /gene_synonym="adhC"
     CDS             1472..4147
                     /gene="adhE"
                     /locus_tag="OO315_004083"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5892766.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEDETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         1169 a         1020 c          959 g         1035 t
ORIGIN      
        1 aattcaatca aatagagatt ggctaagcat gtctatcttt tctgattaca gttccagtag
       61 tgaaatgcac aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag
      121 tggaattact aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa
      181 aataacatga tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag
      241 aagaatattt ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata
      301 atgacgaagt aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg
      361 ccggggaaaa tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat
      421 agccccataa tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac
      481 ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa
      541 ccaaacagat agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc
      601 cctggccccg ccatcaacgg caacgccaat ggcaccacac caatactttc acgtaccgcg
      661 gtttctgatt tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac
      721 atcgctatcg tcaccaccag gatgcccccg gcgatacgga acgaatcaat tgatatacca
      781 aaaagttgta gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac
      841 aggttggctg taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg
      901 ataaagacgg gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg
      961 aagtaaacgg gaaaatcaaa aagggtctga atcacggttc gctccgaagc aaaagccgga
     1021 taatgttagc cataaataag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
     1081 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
     1141 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
     1201 aagcgatgct gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc
     1261 cagaagtgag taatcttgct tacgccacct ggaagtgacg cattagagat aataactcta
     1321 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
     1381 attggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
     1441 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
     1501 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
     1561 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
     1621 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
     1681 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
     1741 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
     1801 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gcctgaagac
     1861 ccgtaacgcc attatcttct ccccgcaccc acgtgcaaaa gatgcaacca acaaagcggc
     1921 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
     1981 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
     2041 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
     2101 cggtgtaggc gcgggtaaca ctccagttgt tattgatgaa actgctgata tcaaacgtgc
     2161 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
     2221 gtctgttgtt gttgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg
     2281 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaatgg
     2341 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
     2401 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
     2461 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
     2521 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
     2581 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
     2641 aacagctcgt atcctgatta acaccccggc ttctcagggt ggtatcggtg acctgtataa
     2701 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc
     2761 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
     2821 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
     2881 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
     2941 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
     3001 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
     3061 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
     3121 cgccgcgaag atcatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
     3181 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
     3241 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
     3301 tgtaactgac gacgctactg gtcagaaata cccgctggcg gactatgcgc tgaccccgga
     3361 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
     3421 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
     3481 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
     3541 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
     3601 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
     3661 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
     3721 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
     3781 ggctcgccgt cgttatgctg aaattgccga ccacctgggt ctgagcgcac cgggcgaccg
     3841 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
     3901 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
     3961 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
     4021 gatctccgag ctgaaacaga ttctgctgga tacctactac ggtcgtgatt atgtagaaga
     4081 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
     4141 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAJTRD010000200         4182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTRD010000200 DAJTRD010000000
VERSION     DAJTRD010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.201"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..186)
                     /locus_tag="OO315_004084"
     CDS             complement(<1..186)
                     /locus_tag="OO315_004084"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5892767.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAEKVKK"
     gene            complement(327..2360)
                     /gene="metG"
                     /locus_tag="OO315_004085"
     CDS             complement(327..2360)
                     /gene="metG"
                     /locus_tag="OO315_004085"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5892768.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2492..3601
                     /gene="apbC"
                     /locus_tag="OO315_004086"
     CDS             2492..3601
                     /gene="apbC"
                     /locus_tag="OO315_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5892769.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3863..4144
                     /locus_tag="OO315_004087"
     CDS             3863..4144
                     /locus_tag="OO315_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5892770.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1016 a         1044 c         1062 g         1060 t
ORIGIN      
        1 cttcttcacc ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga
       61 aaaacttttt atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc
      121 gtttccctgt tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg
      181 tctcattctg catccctata attttatcca gttgaatgca gatgctacca gtatttatac
      241 gggttagaga gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc
      301 tgtcgatgca gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca
      361 tccgggctta gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca
      421 gagataccga agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca
      481 atcagtgcct gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca
      541 ccgccgagat ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt
      601 tcgatcagcg ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga
      661 atcggatcat ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta
      721 gaggcttcca ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac
      781 ggattcactt tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg
      841 aggaaggctt ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagc
      901 acgcggaaca ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc
      961 tgttttgcga ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt
     1021 gccatgattt cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact
     1081 tcagcggcat cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca
     1141 ccgtcaaaac gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc
     1201 acgatatcgg cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg
     1261 cgcgaagaga gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc
     1321 caggtgctgg ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc
     1381 acatagccat gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca
     1441 ggccagaaca ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg
     1501 gtggagtctt tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga
     1561 ttcttgaaag aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc
     1621 ggtgcgttcg gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc
     1681 aggccggatt caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg
     1741 cgggtccatg cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa
     1801 tcacgcatca ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg
     1861 ctgtaggtcg cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc
     1921 gggcaggtgc ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc
     1981 tgagagatgg tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct
     2041 gacaactggc ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag
     2101 cctgcgaaat cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata
     2161 ccgagctgct gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag
     2221 ttgacctcgt ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc
     2281 agcatatggc cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt
     2341 ttcttcgcga cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag
     2401 taccaaatgg gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct
     2461 gatggtttat ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc
     2521 agaagccttg cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa
     2581 gcataacctc accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca
     2641 tgtggaactg gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg
     2701 tagcgccgaa ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa
     2761 catagccact ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat
     2821 cgcggtgagt tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct
     2881 ggcactggcg gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc
     2941 aattcccacc atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat
     3001 ggcaccgatt atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga
     3061 caatgcgatg gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga
     3121 aaccttgtgg ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat
     3181 tcaattgacg ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca
     3241 ggacatcgcg ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc
     3301 ggtgctcggt attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga
     3361 accgattttc ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct
     3421 ggggcagatg ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt
     3481 tatcagccgt ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc
     3541 agctcagctc tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta
     3601 acgcctatta accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt
     3661 gtatttgttt ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct
     3721 tcgtccgaaa ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg
     3781 tgctgtgtag tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc
     3841 tttactacag ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc
     3901 ctatggattg gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag
     3961 gatcagtccc ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg
     4021 tcagttaacg tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac
     4081 gacagaagtg gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga
     4141 ttaatttggc aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAJTRD010000201         4114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTRD010000201 DAJTRD010000000
VERSION     DAJTRD010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.202"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..158)
                     /locus_tag="OO315_004088"
     CDS             complement(<1..158)
                     /locus_tag="OO315_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5892771.1"
                     /translation="MDQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYL"
     gene            complement(308..469)
                     /locus_tag="OO315_004089"
     CDS             complement(308..469)
                     /locus_tag="OO315_004089"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892772.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPASS"
     gene            complement(466..654)
                     /locus_tag="OO315_004090"
     CDS             complement(466..654)
                     /locus_tag="OO315_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5892773.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            914..1249
                     /gene="iraM"
                     /locus_tag="OO315_004091"
     CDS             914..1249
                     /gene="iraM"
                     /locus_tag="OO315_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5892774.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1530..1638)
                     /locus_tag="OO315_004092"
                     /pseudo
     CDS             complement(1530..1638)
                     /locus_tag="OO315_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(2161..2237)
                     /locus_tag="OO315_004093"
     tRNA            complement(2161..2237)
                     /locus_tag="OO315_004093"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2201..2203),aa:Arg,seq:tct)
     gene            complement(2339..2414)
                     /locus_tag="OO315_004094"
     tRNA            complement(2339..2414)
                     /locus_tag="OO315_004094"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Ile,seq:cat)
     gene            complement(2559..3380)
                     /locus_tag="OO315_004095"
     CDS             complement(2559..3380)
                     /locus_tag="OO315_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5892775.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3395..3751)
                     /locus_tag="OO315_004096"
     CDS             complement(3395..3751)
                     /locus_tag="OO315_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5892776.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3764..>4114)
                     /locus_tag="OO315_004097"
     CDS             complement(3764..>4114)
                     /locus_tag="OO315_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5892777.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1155 a         1118 c          793 g         1048 t
ORIGIN      
        1 agatatgtca gcagcccaaa cagcagactc cccagcacgc ctattgccgc ccactgagac
       61 ggggaaaccc tgtccagcaa ctgcaggaac cagtagcccg ttcccaccgc tgacgtggtg
      121 tatgacacac ctgttgtgat tttttccatc tggtccatac cccgtctccc gttatccgga
      181 agctgacaac aataaaaaag ccaccagtta actactgatg gctctgataa ctcatgcaag
      241 cgtctcagac gacccactga cactaccggt gagtttaacg ataccttcca tttgactggc
      301 tcacttttta tgatgatgcc ggtgcattta tctccagcac cagactttct atctcaacgc
      361 catacgttgc atttttggta atatccgtca gcgtcagtgc atttagtccc actgccagac
      421 tgtcttttat ggcctggaat gccgggccag tacgatgacg tagtatcact ccggctcagt
      481 tgcaccactg accaccacat caccttctgc tgcaatcgcc tgcatcaggg tataaggggt
      541 tatggccacc ggactaccaa acggctgcca gccctctttc agtttatgtg tcagcttttc
      601 cgcaagatct gacggcggcg ccgccctgac aacatcatag tgtttaaatg ccatggttct
      661 ttccaacatc tgaaaaataa ttctttaaaa tacctgacat gtaatacaga aaaaacacaa
      721 aaccatacct taaataaaaa cctgattatc aagcagatat gcatggataa actacaagac
      781 gagatataaa ccaccctgca tttaaataaa caataaacaa catcagaaaa ataattctgt
      841 tctatggttt aattcaaaaa tatcatttat acttttcaga acatcaccag caaggcataa
      901 acaaggaaac taaatgaagt ggattgtgat tgatacagtt atccagccat catgcggaat
      961 atctttttca gtcatatgga gtaaaataaa attaataatc tggtatcaat cggatgcttt
     1021 cttacctcct gaaagtatat ttacactgac tcacacaggc atcatgctca ataacaaagt
     1081 gctacctgta accatttaca acgtagtacc attcaataaa acattctgga atttaatcaa
     1141 aaacagccag gaatgcccta caaatacaga taacgtattg aatgaatgct ttaataaccg
     1201 ttgcactctg caaatatgcc cttatggact aaaacaacaa agtccataag gagtttactc
     1261 acatctgaca aaatcaatat aaacagcccc tccggagagg ggctggagag tggcgctatg
     1321 tgccattcca tggtgccggg tgcctcccgg tgaattcagt accagcacct gaatccgcga
     1381 ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac catgccagtt
     1441 tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg tgaagtattt
     1501 aaaaatttct cccgctaact gatacccggc taacagtctg gcgttttctt tttcagcaac
     1561 gggaaagcaa caaccaccac acccgccacc agcacaccgt cagccggcac tgacattatc
     1621 cggctgctgc aatgccatta acaaaaacag taagcaatca ctttttaccg taacaggtga
     1681 taatccagat atgtatctac cccagatgag taatccgaag ttcatccata ccacaggtcc
     1741 tggctattct gttgtactcc tgaacaagag caaataattc tgaattagca accatgaact
     1801 catcgcaaac cctctgtata gcatcactat tcagaataat aacgtctctt cccgaaagac
     1861 gatcaggagt acagaacaaa actgtcaaac ggctgaaggc ctttgctcgt gctgcattga
     1921 ctatatcaat acgctgccta aggatgaaac accccgacgc ctcatcaata ttcactctac
     1981 ccacaccata tgaatgataa atacttaatg ctgaaaaaac cattagaccg tataacaaat
     2041 actcaatcaa tacttaacag aacttttatt tttgacaaat atataatatt ttcaacaata
     2101 tcctgagcca ggtatatttc agtataaggc tctgccggaa ggaatctgga agaatgaata
     2161 tggcgcgctg tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc
     2221 ggctgagcta acaacgctga ataccgataa tggaccgcca tcggggaccc gccccccgca
     2281 ccaacaaccc tgttatcgtg tcgtctgctc ttcctgataa gctaatggcg gtttgtgatg
     2341 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     2401 tgagctaaag ggccgggagc agaataataa cggtccgtaa ttaatttcgc aataaaaaaa
     2461 cccgctcagc ggcgggttgt agaaactctt ctaacgccag gcataaaacg cccatcatta
     2521 tgacgaattt accacagatt ccggaaaaat caaccttgtt acctagttac cttttttaac
     2581 tgccgctcag cccatgcttc ttcaatatca aaccgggtca ccagcgcatc ataaaatttc
     2641 ttaactgttt tttcccatga cgcgcgtgtt atctggtttg tcacctcgca tatagcatta
     2701 aatgcctccg ttgatggtag tctttcatag ccacgaccac cacaacgctg gcagtctctg
     2761 ataacaggca taccacgttt taccgactct tcacgatgaa tggcgacacc acgcccacgg
     2821 caatccttac aggcggtgga aacctcaccc tttccgccac actccggaca ggcaactttt
     2881 accacctccc tgactttttt ccattcttcc cagtaaaacg gatacacacc tttcgtacac
     2941 tttgcccata ccggcggctt accatccgga tactggatct tgtttgtaaa aactatgctt
     3001 tcaataaatt tttccccata gcaacaaggg cactgctttt tactcgctgc gctgcgggca
     3061 taatcctcaa aagcgtacga agccataatg cgcatcacta ccggttttat ttctgccgga
     3121 agttttctca acgccgccac acgatcgcac cgactgagtg cataatctgc cagtaattct
     3181 gttgcccgcg ccctgtcatt catactgatg cccattttcc ccaggaacgc agaaaaaccc
     3241 atctcagccc gattctgtgt catgccctgc gcggccatca catcagtgat actcagcgca
     3301 tcttttgacg ttgaggccga tgcatcggtc aggccagggg attttgggga gtagtatttc
     3361 ggtaaatctt ccagtttcat tttttgacct gctcttaatg cattatgggg taaatcttca
     3421 cccccagacg tccaccagat actggctgac cacgaacgat attgatttca tcaaactgct
     3481 catcgtccat taacactccc gcatgcgtca gcgcatccag cggtgctttc aggatattgt
     3541 ccaggtcgcg acgacgctta tccggtggct ctgcaatcac ctttatcgcc agccttccgg
     3601 acaggcttaa tttcagccgc tgctggcgaa caataagcgc cacagcccgg cgataacgct
     3661 ttccctcctc cgagataaaa tatgtgctgc cacggcgtcg ccagtaagtg ttcaccgtcg
     3721 gcgggtaagg taaaaccaaa tctatgagca tcagtcacct cttttaccca agcacgccag
     3781 ttgcaaaggc gtgatcaaga aaacgaaaaa ttaaatcaac ctgagaacca tgcttttctt
     3841 cgaacgccag aggatccgca tgaagctcgt tgtgatgctc ccgacacagc ggtagcgtga
     3901 aaatatcgtg agatttggtc cccattccgc cctgaccatg accaatcagg tgatggggat
     3961 cgtcggctgg cttaccacaa cacgcacacg gctgtgtctt cacccagcgt ttgtatttct
     4021 cgttaaccca gcggcgacgt ttaggtcgtt tcatgaaaga ttccggagac tcaggatcaa
     4081 cggcaatgct gaccaccgtc ttttcctgtt gtgg
//
LOCUS       DAJTRD010000202         4114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTRD010000202 DAJTRD010000000
VERSION     DAJTRD010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.203"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..196
                     /locus_tag="OO315_004098"
     CDS             <1..196
                     /locus_tag="OO315_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5892778.1"
                     /translation="WPVERYLSLLTGELPRLHDDSDGYGPRGRDFIVHVDFPAEVIHA
                     WQTLKHDAVLIEAMESRSLR"
     gene            393..1832
                     /gene="aldA"
                     /locus_tag="OO315_004099"
     CDS             393..1832
                     /gene="aldA"
                     /locus_tag="OO315_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5892779.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(1874..2874)
                     /gene="gap"
                     /locus_tag="OO315_004100"
                     /pseudo
     CDS             complement(1874..2874)
                     /gene="gap"
                     /locus_tag="OO315_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            3063..3593
                     /gene="cybB"
                     /locus_tag="OO315_004101"
     CDS             3063..3593
                     /gene="cybB"
                     /locus_tag="OO315_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5892780.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3838..4011
                     /locus_tag="OO315_004102"
     CDS             3838..4011
                     /locus_tag="OO315_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892781.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
BASE COUNT         1047 a          937 c         1038 g         1092 t
ORIGIN      
        1 atggccggtt gagcgttatc tctcactact cactggcgag ctgccgcgtt tacacgatga
       61 tagcgatggc tacggtcccc gcgggcgaga ttttatcgtt cacgttgatt ttccggcaga
      121 agtcatccat gcatggcaaa cgctgaaaca tgatgcggtg ctcatcgagg cgatggaaag
      181 tcgctcgtta cgttaaaaat tgcccgtttg tgaaccactt gtttgcaaac gggcatgact
      241 cctgactttt atttctgcct tttattcctt ttacacttgt ttttatgaag ccctccacag
      301 acttgtcctt tcccgatgcc gtctctctga tgattgatgt taattaacaa tatattcacc
      361 gaaaacaaac atataaatca caagagtcgc ccatgtcagt acccgttcaa catcctatgt
      421 atatcgatgg acagtttgtt acctggcgtg gagacgcatg gattgatgtg gtaaaccctg
      481 ctacagaggc tgtcatttcc cgcatacccg atggtcaggc cgaggatgcc cgtaaggcaa
      541 tcgatgcagc agaacgtgca caaccagaat gggaagcgtt gcctgctatt gaacgcgcca
      601 gttggttgtg caaaatctcc gccgggatcc gcgaacgcgc cagtgaaatc agtgcgctga
      661 ttgttgaaga agggggcaag atccagcagc tagctgaagt cgaagtggct tttactgccg
      721 actatatcga ttacatggcg gagtgggcac ggcgttacga gggcgagatt attcaaagcg
      781 atcgtccggg agaaaatatt cttctgttta aacgtgcgct tggtgtgact accggcattc
      841 tgccgtggaa ctttccgttc ttcctcattg cccgcaaaat ggctcccgct cttttgaccg
      901 gtaataccat cgtcattaaa cccagtgaat ttacgccaaa caatgcgatt gcattcgcca
      961 aaatcgtcga tgaaataggc cttccgcgcg gcgtgtttaa ccttgtactg gggcgtggtg
     1021 aaaccgttgg gcaagaactg gcgggtaacc caaaggtcgc aatggtcagt atgacaggca
     1081 gcgtctctgc aggtgagaag atcatggcga ctgcggcgaa aaacatcacc aaagtgtgtc
     1141 tggaattggg tggtaaagca ccggctatcg taatggacga tgccgatctt gaactggcag
     1201 tcaaagccat cgttgattca cgcgtcatta atagtgggca agtgtgtaac tgtgcagaac
     1261 gtgtttatgt acagaaaggc atttatgatc agttcgtcaa tctgctgggt gaagcgttgc
     1321 aggcggttca atttggtaac cccgctgaac gcaacgacat tgcgatgggg ccgttgatta
     1381 acgccgcggc gctggaaagg gttgagcaaa aagtggcgcg cgcagtagaa gaaggggcga
     1441 gagtggcgtt gggtggcaaa gcggtagagg ggaaaggata ttattatccg ccgacattgc
     1501 tgctggatgt tcgccaggaa atgtcgatta tgcatgagga aacctttggc ccggtgctgc
     1561 cagttgtcgc atttgacacg ctggaagatg ctatctcaat ggctaatgac agtgattacg
     1621 gcctgacctc atcaatctat acccaaaatc tgaacgtcgc gatgaaagcc attaaagggc
     1681 tgaagtttgg tgaaacttac atcaaccgtg aaaacttcga agctatgcaa ggtttccacg
     1741 ccggatggcg taaatccggt attggcggcg cagatggtaa acatggcctg catgaatatc
     1801 tgcagaccca ggtggtttat ttacagtctt aatgagtgaa agaggcggag gttttttcct
     1861 ccgcctgtgc gcgtcagagt ttagcgaatt tttcgagggt gcgaataagc tgcgtgacga
     1921 agccatattc gttatcgtac caggcgaccg ttttcaccag ttgtaaatcg cccacggcgg
     1981 taatttccgt ttgcgtggca tcaaacactg aaccgaaatg gctgccaatg atatcggaag
     2041 agactatttc ttcatcggta taaccaaatg actcgttatt ggtggttgct tgtttaagtg
     2101 cgttattcac ctcttcggca gtcacttttt tccgagaatg gacaccagct cagtgaccga
     2161 acctgttttc accggcacgc gttgcgcatg acctttcagt ttgccgctca gttccgggat
     2221 caccagacca atggcttttg ccgcccccgt agtgtgggga atgatatttt ctgccgctgc
     2281 gcgtgaagca cgtaaatctt taccacgcgg gccatccacc agtgactggg tgccagtata
     2341 ggcatgaatg gtcgtcatcg tgccgacttc tatcccgaaa ctgtcatgca aggctttggc
     2401 catcggcgca aggcagttag tggtgcatga cgccacggaa acaatggtgt cgttgccatc
     2461 cagagtgtcg tcattaacgt tataaacgat ggttttcatt tcaccggcag gagcggaaat
     2521 caacaccttc ttcgcaccag catcaagatg cgcctgcgat ttatcggcgg aggtataaaa
     2581 gccagtacat tcgacaatga tttctgcccc tttcgctttc cacggaatat ttttagcctc
     2641 tttttcggcg taaaccgcga tacttttccc atcaacgata agtgaatctt ccgtaaaatc
     2701 aacgctccag gggaacggac catagtttga atcatgtttc agcagatagg cgagaatttt
     2761 tggggaagta agatcattaa tagcgacaac gtctatgttg cttttgactt caagtaaacg
     2821 acgcaacacc agtcgaccga tacgaccaaa accgttaata ccaactttac tcatggtttt
     2881 ctcctgtcag gaacgttcgg atgaaaattg atcctttcca ggcttagacc aggattgtgg
     2941 gatgggcaat ctccattctt acagtgaaac gtaacgtact gaaaacgggt gaacaatatt
     3001 taatgaaatt ttgagaaaag cccgttatgt taacggaaaa ttatgttaaa gcaggaaatg
     3061 ttatggaaaa taaatactca aggttacaaa tcagcattca ctggctggtc tttttgctgg
     3121 ttatcgcagc gtattgcgca atggagtttc gtggtttctt cccacgtagc gatcggccgc
     3181 tcatcaacat gattcatgtt tcctgtggca tctcaattct cgtgctgatg gtggttcgtc
     3241 ttttgttaag actgaaatac ccaaccccgc cgattatacc taagccgaaa ccgatgatga
     3301 cgggactggc gcatttggga catttggtga tttatctcct gtttattgcg ctgccagtga
     3361 ttggtttggt gatgatgtat aaccggggca acccgtggtt tgcgtttggt ttgacgatgc
     3421 cttacgcttc agaggccaat ttcgaacggg ttgatagctt aaagtcgtgg catgagacgc
     3481 tggcgaatct gggatatttt gtcatcgggt tgcacgctgc ggctgcactg gcacaccact
     3541 atttctggaa ggacaacaca cttctacgca tgatgccgcg taaacgttcc tgaaggatat
     3601 ttaaagaaaa cgcctgtact aaaaccgacc cgtggtacag gcgaagaata cgggtctaca
     3661 tcggaagcgc ctattatatt tatttgtatg acaaataaaa ccataatcct tgcccatacg
     3721 tccatctggc ttatttttaa tcaattcacc cgatctttga tctcatcaac ggtatcaaaa
     3781 taaaccagcg tataacgttc aaatcgcaca taagattcga taaaccatgg aggttatatg
     3841 aaaaaactgg cacttatttt gtttatggga acgcttgttt ccttttatgc cgatgccggg
     3901 cgcaaaccct gttctggttc gaaagggggg atctcacact gtacggcagg cggcaaattt
     3961 gtctgtaatg atggttctat tagtgcatcg aaaaaaacat gcactaactg aagtgtaaaa
     4021 ggggtgccat gagaaaatga atctgctagt cagatgcgcg gggaaaatcc ccgcgcttgc
     4081 ccttacctgg acgtgcaggc catgagcgca gcaa
//
LOCUS       DAJTRD010000203         4043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTRD010000203 DAJTRD010000000
VERSION     DAJTRD010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.204"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(886..1077)
                     /locus_tag="OO315_004103"
     CDS             complement(886..1077)
                     /locus_tag="OO315_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5892782.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENR"
     gene            1142..1465
                     /locus_tag="OO315_004104"
     CDS             1142..1465
                     /locus_tag="OO315_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5892783.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OO315_004105"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OO315_004105"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OO315_004106"
     CDS             2143..2673
                     /locus_tag="OO315_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5892784.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTHWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OO315_004107"
     CDS             2680..2913
                     /locus_tag="OO315_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5892785.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3460..3852)
                     /locus_tag="OO315_004108"
     CDS             complement(3460..3852)
                     /locus_tag="OO315_004108"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892786.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          918 c         1208 g          951 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcagc ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 cactggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggg caccgggaac gcgcttcagt cagaccttgc
     3121 tgaaaacaca ggaacgagtt aaaacgcata cgggcgcgat tctggtgtgt cttttgtcgc
     3181 tgtgttttgc ctctattgcc agatcgcgct cattcccctg aacttctgtt ttcccgctca
     3241 ggtccccgtc ttcttcgctt ccgaaagcat gaataaaatc cgtttcagct gtctctggct
     3301 ggcaattcct gcgtgattca catggctgca tagctatgca tgtggttaaa atttaccggg
     3361 tgcgatcgcg gcagtttttc gggtggtttg ttgccatttt tacctgtctg ctgccgtgat
     3421 cgcgccgaac gcgtttcagc ggtgcgtaca attaagatat tacgttaaat tgtgagggaa
     3481 tggtccccat atactgactt tatcataggg atagggttgg tagtgggtcg attgggtttc
     3541 ggcattgttt ccgttaatgc caattgcatt tttgtatttg atgcaacaga aggcgtgaga
     3601 ttctcctggg cctggaaagg ctaagtgttt agaattgatg gcaaaatatc ttccgtccga
     3661 tttttgaagt agttgttgtt tggctgtctc ccaaagggtg ctgttacaaa cttcttgaat
     3721 ttgtagtttt tgcaggactc ttattactgc gttgtcatgt tccaattctt taggggagtg
     3781 tattattctc tcttttaata agctgtttac cacagggttt agttgttgtc taaagcacgc
     3841 agataatgcc attgctacac aattttgttc ccgaggaagc atgtttggcg gggtatgccg
     3901 tgtttgatgt gatatgtcta ttctcataga aggtctctgg cttatagcat caacaaatac
     3961 ggctataaaa caaaattttc atagagtgat tcaagatctt cttgagatcc ttttttgcgg
     4021 cgcgtaatct gctgcctgca aac
//
LOCUS       DAJTRD010000204         3834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTRD010000204 DAJTRD010000000
VERSION     DAJTRD010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.205"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..103
                     /locus_tag="OO315_004109"
     CDS             <1..103
                     /locus_tag="OO315_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5892787.1"
                     /translation="AKIQDYIKHQLEEDKMGEQLSIPCPGSPFTDRK"
     gene            complement(221..1165)
                     /gene="hypT"
                     /locus_tag="OO315_004110"
                     /gene_synonym="qseD"
     CDS             complement(221..1165)
                     /gene="hypT"
                     /locus_tag="OO315_004110"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001333359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5892788.1"
                     /translation="MVMKNIETKWLYDFLTLEACRHFSQAAKERNLSQPAFSRRIKAL
                     EAAIGVVLFDRTTTPLQLTEEGKLFHSQTRSLLQQLECNLGELNGQSLLGVPNIKIAA
                     AHSLSLSVLPKLVHSLTAYGGEFVYHVEAIDVVQAVNTLREGKSDFIISFRDEDLMQS
                     PFCCLKLFESELYPVCAADAQGHPVFDITQPQVPLLNYTATSYMGRLVNRHLAEVGGI
                     TGRTIFISSMSELLKNMALNGYGIAWLPIWSIVDELQTKRLICLDAAKLTVPIQAYIY
                     RMNTRLNRTAENLWRILQEHMPDDLIQQISMEEPARRN"
     gene            1510..2229
                     /locus_tag="OO315_004111"
     CDS             1510..2229
                     /locus_tag="OO315_004111"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016156986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5892789.1"
                     /translation="MKGLIGVLGGMGPAATVDLFNKFVTFTAAQCDQEHIPLIISSIP
                     DIPDRTDALMHHGRSPLPVMRDYMHKLEDAGAECIVIPCNTAHYWFKELKDACHIDIL
                     SIVETTINEVRACGKTRIGLLATNATLYMGLYQKGIESLGFTCVSPDADGQKKVMESI
                     YSLKAGNIAHAQKLMNEQAEILFSRGAEILVLGCTEIPVILAQAVKEQPSRYIDSTAS
                     LVRAGIKWYENRVGKHYLLTQ"
     gene            2295..3593
                     /locus_tag="OO315_004112"
     CDS             2295..3593
                     /locus_tag="OO315_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter"
                     /protein_id="HCR5892790.1"
                     /translation="MVWLEIIVVLGAIFFGIRQGGIGIGLCGGLGLAILTLGFGLPMG
                     SPPVDVILIIMTVVVAASALQAAGGMDYLVRVAGNFMRRNPKYINIIAPIITWSMTIM
                     AGTGFIVFSTLPVIAEVAKESGIRPSRTLAGSVVASQVAISGSPISAAMAAMLTIMEV
                     NGVSFIQVMSVCLPTSFAAAMVAAFIASRQGCELQDDEVYLERLQKGLVQKYENNSSI
                     KPGATLSVGLFLLATVAIVILAAFPQLRPGFDIGKPMETRDIIIICMLSAACLMVVLC
                     KTATDAIILTSTFRAGMSSLAVILGIVTLGTTFIDAHLTEIKDIAGDILQAYPMLLAL
                     VLFGTCALLYSQGATTPLIIPLAVALDVPTWAILASYVAVTGVFVLPTYPTSLAAMEF
                     DTTGTTRVGKYVLNHPFMLPGLGGIIAGVALGFVIAPMII"
BASE COUNT          949 a          846 c          932 g         1107 t
ORIGIN      
        1 ggcgaagata caggattaca taaagcacca gcttgaagag gataaaatgg gagagcagtt
       61 atcgattccc tgtccgggca gcccgtttac ggaccgtaag taacgaagtt ggatgcaaat
      121 gtcagatcgt gtgcgcctgt tagggcgcgg ctggtaagag agccttatag gcgcatttga
      181 aaaacctccg gctatgccgg aggatattta ttgcacgtcg tcaattgcgg cgtgctggct
      241 cttccattga tatctgctgg attaaatcgt ctggcatatg ttcctgtaaa atacgccaga
      301 ggttctcggc ggttctattc agtcgggtgt tcattcggta aatataagcc tgaatgggga
      361 ctgtcaattt agcagcatca aggcatatca gacgtttggt ctgtagttca tcgacgatcg
      421 accagatcgg taaccaggca atgccgtatc cgttgagcgc catattcttt aataactcgc
      481 tcatcgaaga gataaatatc gtgcgcccgg taatacctcc aacctctgcc agatgacgat
      541 tgaccaacct gcccatataa gatgtggcgg tatagttgag caaaggaacc tggggctgag
      601 tgatatcgaa tacagggtgc ccctgtgcgt cggcggcaca taccgggtac aactcagatt
      661 caaacagttt caggcagcaa aaaggcgatt gcataagatc ttcatcgcga aacgaaataa
      721 taaagtcact cttgccttcg cgtaacgtat ttacggcctg gacaacatca atggcttcaa
      781 catgatagac aaattctccg ccataggcgg taagtgaatg caccagtttg ggtaatacgc
      841 tcagggatag tgagtgcgcg gctgcaattt taatgttggg aacgccgagt aaactctggc
      901 cgtttaactc ccccaaattg cattcaagct gctgtaataa gctgcgagtc tgggaatgaa
      961 agagcttgcc ctcttcggtt aattgtaaag gggtggtcgt gcgatcgaat aaaacaacgc
     1021 cgatcgctgc ctccagcgcc ttaattcgtc ggctgaatgc gggttgcgaa agattacgct
     1081 cttttgccgc ctgagaaaaa tggcgacagg cttccagtgt caggaaatcg tataaccatt
     1141 ttgtctcgat attcttcatt accatggcac ctgtattttc aatccaaaga cataataaaa
     1201 catcccggca ataaaaataa tgttctcaat tagcacaacc tggcctttat cattcgtttt
     1261 gtgcataacc ccttcatttc cggtattgac gttgatatca catttcgcca aaaaaacgaa
     1321 atttttccgt cagataacca gaatacatca tcactatggt gaataagcgg aatgttccat
     1381 agctgcatca tgcgtgtctt gcatcgcctt gtacaacctt gcatttctca tcgcccattt
     1441 cgactgttaa aaaagagagc agcagttatt taccgcgtat tgtcattaac gttttgagag
     1501 gcaggtattg tgaaaggttt aattggtgta ctcggtggaa tggggccggc agctaccgtc
     1561 gacctgttta ataaatttgt aacttttacc gctgcacaat gtgatcagga acatattcct
     1621 ttaattattt cttcgatccc ggatattcct gaccgtaccg atgcattaat gcatcatggg
     1681 cgttcaccat tacccgttat gcgtgattat atgcataagc tggaagatgc cggggcagaa
     1741 tgtattgtta ttccctgtaa taccgcgcat tactggttca aagaattaaa agatgcttgt
     1801 catattgata tattaagtat cgtagaaact acgatcaatg aagttcgcgc ctgtggtaaa
     1861 acccgaattg gcttgttagc caccaatgcc acgctatata tgggtttata ccaaaaagga
     1921 attgagtcgc tgggatttac ctgtgtcagc cccgatgccg acggtcagaa aaaagtgatg
     1981 gaaagtattt attccttgaa agctggcaat atagcgcacg cacaaaagtt gatgaatgag
     2041 caggctgaaa tattattttc ccgcggtgca gaaatccttg ttcttggttg tacagaaatt
     2101 ccggttattc tggcgcaagc cgttaaagag cagccttccc gctatattga ctcaacggcg
     2161 tcactcgttc gtgccggaat aaaatggtac gaaaatcgtg tcggtaaaca ttatctttta
     2221 acccaataat catttaaatc gcagccagaa agttattcgc ttttaactga attatattta
     2281 taacggagaa cattatggtt tggctggaaa ttatcgtagt acttggtgca atattttttg
     2341 gtattcgcca ggggggaatc ggtattggtt tatgtggcgg gcttgggctt gccattctga
     2401 ctctgggatt tggtctgcct atggggtcgc cgccagtcga cgtgatcctg attattatga
     2461 ccgttgttgt ggcggcctca gccttacagg ccgcaggcgg aatggattat ctggtacgcg
     2521 tcgctggtaa ttttatgcgg cgtaacccga aatatatcaa cataatcgcg ccgattatca
     2581 cctggtctat gaccattatg gccggtactg ggtttattgt tttttccacg ctgccggtga
     2641 ttgctgaagt ggcgaaagag tccggtatcc gtccttcccg cacgcttgcc ggttcggttg
     2701 tcgcttcgca ggttgcgatt tccggttcgc cgatcagcgc cgcaatggcg gctatgctca
     2761 ctattatgga ggtaaacggt gtcagtttta ttcaagtgat gtcggtatgt cttcccacct
     2821 cttttgctgc cgcgatggtc gccgctttta ttgcttcccg acagggatgt gagttgcagg
     2881 atgacgaggt ctaccttgag cgcctgcaaa aaggactggt gcaaaaatac gagaataata
     2941 gcagcatcaa gccaggtgcg acgctctccg tcggattgtt cctgctggcg acggtcgcta
     3001 ttgttattct tgctgccttc ccccaactgc gtccgggttt tgatatcggt aaaccgatgg
     3061 aaacacgcga tatcattatt atctgtatgc tctctgccgc ctgcctgatg gtggtgctgt
     3121 gtaagacggc gaccgatgcc attattctca cctccacatt ccgtgcgggg atgagttcgc
     3181 tggcggtaat tctcggcatt gtaaccttag gcaccacttt tattgatgcg catttaactg
     3241 agattaaaga tattgctggt gatattttgc aggcgtatcc gatgttgttg gcgctggttc
     3301 tttttggcac ttgcgcgctg ctctactcgc aaggtgcgac cacgccgtta attatcccgc
     3361 ttgccgtcgc gcttgacgtg cccacctggg caattctggc gtcctatgtg gccgtcaccg
     3421 gagtatttgt actgccaacg tatccaacat cactggctgc catggaattt gatactaccg
     3481 gcacaactcg tgtaggcaaa tatgtgctga atcatccatt tatgctgcct gggttggggg
     3541 gaattattgc tggagtggca ttaggctttg tgattgcgcc aatgattatt tagcactatc
     3601 aaacatcagt atcgatggtt tatacacaat aaagacagat atggcggaat atagtctgtt
     3661 gtaagcgcca acaaagaata ggggaattcc gccatgtctg aacatcttca caatatggta
     3721 ttaagtgttg tcgtttaatg atttatttct tggttatgtt gtgtattagc tgcatctatc
     3781 gctttttgat acctgaggtg tactggcaat agcggacact accatttgtt cttt
//
LOCUS       DAJTRD010000205         3806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTRD010000205 DAJTRD010000000
VERSION     DAJTRD010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.206"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            437..2089
                     /locus_tag="OO315_004113"
     CDS             437..2089
                     /locus_tag="OO315_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5892791.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO315_004114"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO315_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO315_004115"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO315_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2596..3526)
                     /locus_tag="OO315_004116"
                     /pseudo
     CDS             complement(2596..3526)
                     /locus_tag="OO315_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766279.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT         1052 a          878 c          874 g         1002 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc aatccagcct ctcactatcg cattgtagcg
     2821 cctcgcaaaa tccagcaaac tctcagctgt tgagcgatgt atacgccact tcttgattgt
     2881 ttctgttatt ttgcacattg ctgcatttga tgcacccggc atacattttc tgtacaattc
     2941 gcctttgaaa ttcttcagag tacgcacttt gaagtcatat ccgagagtgg catacaacat
     3001 catcggcgta cctttcgaag ggaacgccgg gaaactgacg ttgcatccac atatcaaagg
     3061 catagtgcag gaacagattt gagagcaggg gggagattac gccgccctgc ggcgttcctc
     3121 gaccccgtgc cagcattgct ccatccggca tttgcaccgg tgccttcagc ctgcgctcaa
     3181 tgtacatcac cacccagcgt gcctgagtat gatgctgcac tgtcttcagt agcaggtcat
     3241 gatcgatatc atcaacgaag cctttaatat cgacttccac cacccagtca aacttccagc
     3301 agcgtttcct cgcctgcgca atcgcctggt gtgcggattt acccggtcga taaccatacg
     3361 agtctgcatg gaacaggggt tccagtaccg gctcgagtat ctgcttaacg accatctggg
     3421 cgatccggtc cgacacggcc ggaataccga gcggacgtac accaccgtca gctttgggaa
     3481 tgtccacacg ccttaccgcc tgcggcatat aactgccgga agccattctg ttccacagtt
     3541 tgtagagatt gtctgtaaca ttctcgtcaa acccggccag tgtctgcccg tcaatgcctg
     3601 ccgccccacg gttggctttt actcgtttat aagcttccca tacctgtcgt ttacttacag
     3661 gaaatggctt tgccgatttt acagcagtcc tcccatcggc attgtcgccc gtggttgccg
     3721 ccgcaccatc agcacaatgg ggcaagccct ttgctccgga cccattacgg tccttcaccg
     3781 ctactacggt ttactccgtc cctgta
//
LOCUS       DAJTRD010000206         3690 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTRD010000206 DAJTRD010000000
VERSION     DAJTRD010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.207"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..224
                     /locus_tag="OO315_004117"
     CDS             <1..224
                     /locus_tag="OO315_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5892792.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            270..479
                     /gene="hha"
                     /locus_tag="OO315_004118"
     CDS             270..479
                     /gene="hha"
                     /locus_tag="OO315_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5892793.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            518..1108
                     /locus_tag="OO315_004119"
     CDS             518..1108
                     /locus_tag="OO315_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5892794.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1348..1608
                     /gene="repA"
                     /locus_tag="OO315_004120"
     CDS             1348..1608
                     /gene="repA"
                     /locus_tag="OO315_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5892795.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1832..1906
                     /gene="tap"
                     /locus_tag="OO315_004121"
     CDS             1832..1906
                     /gene="tap"
                     /locus_tag="OO315_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5892796.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1899..2756
                     /gene="repA"
                     /locus_tag="OO315_004122"
     CDS             1899..2756
                     /gene="repA"
                     /locus_tag="OO315_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5892797.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3457..3648
                     /locus_tag="OO315_004123"
     CDS             3457..3648
                     /locus_tag="OO315_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892798.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          998 a          901 c          928 g          863 t
ORIGIN      
        1 attttcagcg agatcgttat ggtcgtattc tcgggcaggt ttatgcacca gacggaatga
       61 atattaatca gtttatggtc cgggccgggg ctgcctgggt atatgagcag tacaacacag
      121 atcccgtgct acctgtcctt cagaacgaag ccagacaaca aaaacgtggg ctctggtcag
      181 atgctgatcc ggttcccccc tggatatgga ggcaccgtaa ataaagcgat ggcaatccta
      241 tcattcatct gttctcagcg ggaataaaaa tggcaaaaac aaaacaggaa tggctgtatc
      301 agttacggcg atgttcatca gtcaatacgc tggaaaaaat tattcataaa aacagagaca
      361 gtttatcaaa cagtgaacgt gaatcattta attcagcagc agaccaccgg ctagcggaat
      421 taatcaccgg aaagttatat gacaggatac caaaagagat atggaaatat gtaaggtaat
      481 aattcagcaa taattaattg ccaggagcag ataaataatg aagttgatta tttttattct
      541 cattgttctg attattgcag cgctacttat ccggattatc ctcagaagtg taaatcagca
      601 ttctcctttg ctgatgcaat tgcatgcggc aggtatacgg actggtgatg cagagcgaat
      661 actgtccagc ggtgaatact ggcaacgtca gaagacgctg ctgacagaaa gggaagtcag
      721 ttttatgaaa ggactgttca gaattgtgga tatgaagcgg tggtatctgt gtccgcaggt
      781 acgggtagcg gatatcgtcc agctgaacgg gaatatccgg ccacgatcac gccagtggtg
      841 gcagttattc aggatggtgt ctcagtggca tgttgatgtg gttatcgttg agctgcgttc
      901 gttcagtatt gttgcagcag tagagctgga tgatgccagc catttacgac cggaacgcag
      961 acgccgggat attcttctgg aagaggttct gaggcaggct ggtattccgt tgctcagaag
     1021 ccacgatgcc agaaaactgc tgcagatgac cggagaatgg ctgaatacaa caggggctga
     1081 tcagcagtca ccggaacatc gtagctgacg ccttcgcgtt gctcagttgt ccaaccccgg
     1141 aaacgggaaa aagcaagttt tcctcgctcc cggcgtttcc ataactgaaa accatactat
     1201 ttcacagttt aaatcacatt aaacgacagt aatccccgtt gatttgtgcg ccaacacaga
     1261 tcttcgccac aattctcaag tcgctgattt caaaaaactg tagtatcctc tgcgaaacga
     1321 tccctgtttg agtattgagg aggcgagatg tcgcagacag aaaatgcagt gacttcctca
     1381 ttgagtcaaa agcggtttgt gcgcagaggt aagcctatga ctgactctga gaaacaaatg
     1441 gccgctgttg caagaaaacg tcttacacac aaagagataa aagtttttgt caaaaatcct
     1501 ctgaaagatc tcatggttga gtactgcgag agagagggga taacacaggc tcagttcgtt
     1561 gagaaaatca tcaaagatga actgcagaga ctggatatac taaagtaaag actttacttt
     1621 gtggcgtagc atgctagatt actgatcgtt taaggaattt tatggctggc cacgccataa
     1681 ggtggcaggg aactggttct gatgtggatg tacaggagcc agaaaagcaa aaaccccgat
     1741 aatcttcttt aactttggcg agtcagaaag attaccgggg cccacttaaa ccgtatagcc
     1801 aacaatcaag ctatgcgggg agtatagtta tatgcccgga aaagttcaag acttctttct
     1861 gtgctcgctc cttctgcgca ttgtaagtgc aggatggtgt gactgatctt caacaaactt
     1921 attaccgcca ggtaaagaac ccgaatccgg tgttcacacc ccgtgaaggt gcccggacgc
     1981 ttccgttctg cgggaaactg atggaaaagg cggtgggctt cacctcccgt tttgatttcg
     2041 ccattcatgt ggcacatgcc cgttcgcttg gtctgcgtcg gcgcatgcca ccggtactac
     2101 gtcgccgggc cattgatgcg ctgctgcagg ggctgtgttt tcactatgac ccactggcca
     2161 accgcgtcca gtgttccatc accacgctgg ccattgagtg cggactggcg acggagtctg
     2221 ctgccggaaa actctccatc acccgggcca cccgggccct gacgttcctt gcagagctgg
     2281 gactgattac ctaccagacg gaatatgacc cgcttatcgg gtgctacatt ccgaccgata
     2341 tcacgttcac atctgcactg tttgctgccc tcgatgtatc agaggaggca gtggccgccg
     2401 cgcgccgcag tcgtgtggaa tgggaaaaca gacagcgcaa aaagcagggg ctggataccc
     2461 tgggtatgga tgaactgatg gcgaaagcct ggcgttttgt tcgtgagcgt ttccgcagtt
     2521 accagacaga gcttaagtcc cgtggaatga agcgtgcccg tgcgcgtcgt gatgcggaca
     2581 ggcagcgtca ggatattgtc accctggtga aacggcaact gacgcgtgaa atctcggaag
     2641 ggcgcttcac tgccagtcgt gaggcggtaa aacgtgaagt ggagcgtcgt gtgaaggagc
     2701 gcatgattct gtcacgtaac cgtaattaca gccggctggc cacagcttcc ccctgaaagt
     2761 tacctcctca gaataatccg gcacgcgccg gaggcattcg cacgcctgaa gcccgccagt
     2821 gcacaaaaaa tcagcgctac atacaaaaaa caacctcatc atccaccttc aggtgcatcc
     2881 ggtcccccct gtttttgata caaaacacgc ctcacagacg gggaattttg cttatccaca
     2941 ttaaactgca agggacttca ccataaggtt acaaccgttc acgtcataaa gcgccagacg
     3001 tcaaccctac agggtgcaat gtatctttta aacacctgtt tatatctcct ttaaactact
     3061 taattacatt catttaaaaa gaaaacctgt tcactgcctg tcctgtggac agacaaatat
     3121 gcacctccca ccgcaagcgg cgggccccca accgaagccg gtttagttac aacatgcaaa
     3181 cataaacccc taaaaaaccc aagtccaacg caaaactgaa accacaacgc ccccctcata
     3241 actgaaaagc gccgccgccc ccgcccaaag ggcgggaaca gagtcgcttt taattatgaa
     3301 tgttgtaact acatcatcat cgctgtcagt cttctggctg gacgtatcga atacacgctc
     3361 gtaagcggcc ctcacggccc gctaacgcgg agatacgccc cgacttcggg taaaaccctc
     3421 gtcgggacca ctccgaccgc gcacagaagc tctatcatgg ctgaaaacgg gtatggcttg
     3481 gcagggctgg ggatgggtaa ggtgaaatct gtcaatcagt accggctgac gccgggcttc
     3541 ggcggtttta ctccggtatc acatgtaacc acagcgtgcc gccttccatg ccgctggcgc
     3601 ggcatcagaa taatacaagc agcatttaat gcatttgcaa aagtgtgagt aatattaagt
     3661 ggtcgtttca gggatatcgt taagacttga
//
LOCUS       DAJTRD010000207         3615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTRD010000207 DAJTRD010000000
VERSION     DAJTRD010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.208"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..209)
                     /locus_tag="OO315_004124"
     CDS             complement(<1..209)
                     /locus_tag="OO315_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HCR5892799.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTR"
     gene            complement(364..522)
                     /locus_tag="OO315_004125"
     CDS             complement(364..522)
                     /locus_tag="OO315_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5892800.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(593..811)
                     /locus_tag="OO315_004126"
                     /pseudo
     CDS             complement(593..811)
                     /locus_tag="OO315_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
     gene            1006..2547
                     /gene="istA"
                     /locus_tag="OO315_004127"
     CDS             1006..2547
                     /gene="istA"
                     /locus_tag="OO315_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family transposase"
                     /protein_id="HCR5892801.1"
                     /translation="MPTVPISMRKLKEILRLKYGVGLSHRQIGRSLAISPSVVSRYAN
                     RAAQLGIKQWPLPTGWDDTKLKHAFLQTQVKMKKHSLPDWATVHRELRNKCVTLQLLW
                     EEYCERNPGGFYSYNHYCRMYREWLKTTSPSMRQVHKAGEKLFVDYCGPTVGVTDPET
                     GEIRTAQVIVAVLGASSYTWAEATWSQQLEDWVMSHVRCFQWLGGVPELVVPDNLKSA
                     TSRACKYDPDVNPTYQQMLEHYNVAVLPARPRKPKDKAKAEVGVQVVERWIMARIRHE
                     IFYSLASLNQRIRELLERLNNKIMQKLGYSRAELFIQLDKPALKPLPEASYSYTLVKK
                     VRVHADYHVEIDKHYYSVPCSLLGQQLEAWISGELVRLFNQGQEVAVHPRKRTYGYST
                     RNEHMPEAHRQHATWTPERLLEWAGHIGSETHSYVLHILNSRPHPEQSYRFCLGLLNL
                     HKKYSKARLNAACARALKTKVWRLSGIKSILEKGLDKQPVQDPKPDLLSTMEHENVRG
                     SEYYH"
     gene            2562..3308
                     /gene="istB"
                     /locus_tag="OO315_004128"
     CDS             2562..3308
                     /gene="istB"
                     /locus_tag="OO315_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc12 family helper ATPase
                     IstB"
                     /protein_id="HCR5892802.1"
                     /translation="MNHLYEQLTALKLTGFRDALKKQLAQPGTYQELGFEERLSLLTA
                     EELTCRENRKAERLIKHARFRLNAELSKLDYRNNRGLDRALIRSLSQGNWLTLKQNIL
                     LTGATGSGKTFLACALGHNACRQGYKVYYYRLKALMEQCYQGHADGRYSKLLTRLNNS
                     DLLLLDDWGLEPLSSEQRSDLLEIVDLMYQRGSIIVVSQLPVENWYKMIGDSTHADAI
                     LDRLVHGSIKIELKGESMRKIQSPLTEGDQ"
     gene            3394..3615
                     /locus_tag="OO315_004129"
                     /pseudo
     CDS             3394..3615
                     /locus_tag="OO315_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          991 a          858 c          904 g          862 t
ORIGIN      
        1 cgggtctggc aggcgaagaa tggctgaaaa ccttcctgct gcaggctttc cagtacggtg
       61 atggtgggaa tgtacgcata tcgtttactg cgggaagtat gccggtcttc accgaaaata
      121 ctgggaacat agtgcatcag ttcttcgtgt gtcagcggac ggtcacggcg tatctggttc
      181 gcataaccaa aacgactggc tagtcgcata atttgctcct tatcggtggt tacgatttac
      241 tggtgtaata aatgaaaaag ctgcgtctcc cggagaagac gcggcctgac agatgaaatg
      301 aatgacgttt attgtctgag aagcccttaa ctggcgagct gagtattaag ctgtgttccg
      361 gcatcaccag cgcaactgac cttcagcatt acggataacc agccgggaat atgttccctg
      421 gtcatcttca gtaaacacat tgcggtaagc tgttttgacg gcaacagcct gttcgcgtga
      481 gaaagggcct tcgggcaata aacgtgatgt acaacccggc atatctgttg ctccctgaaa
      541 gtaaaaagcc ccggtcatga tgaccggggc ctgaaggaga gtgacctgat tatcaaaaag
      601 tcacattcag cgtggcctga ccgttataac cttcagcgct gctgccgctg acgctgtggg
      661 cataaccggc ctgaacgccc agggtgatat tttcccggac acgggcttcc agtccggcct
      721 gcaggtccag tgacgtgcca ttccgggacg gtgagaacgt catgttactg ccggcggcgg
      781 ctgtccccat gctcatgtca ccccgggagc tgtgcgtatt ttcatgaaag gagatcactc
      841 aataacttcc atcgagatcg ggtaataaca tttgaacaga tcgctgaata acatcgatgg
      901 agatcacttt tgactcattt tgttattcag tgatctccat caatgttatt ggaacttcac
      961 aggtgtgttg atctgtatct tttgccattc cggtaaagga tacctatgcc aacagttcca
     1021 atttctatga gaaaacttaa agaaattctt aggcttaaat acggtgttgg actcagccat
     1081 cgacaaattg gtcgtagtct tgcaatctcc ccttccgttg tatccagata tgctaatcgg
     1141 gcggctcaac ttggcataaa gcagtggccc ttacctacag gatgggatga tacaaaacta
     1201 aaacatgcgt tccttcagac ccaggttaag atgaagaagc actctctgcc tgactgggct
     1261 acagtacacc gggaactgcg taataaatgc gtgacgctgc agctactctg ggaagaatac
     1321 tgtgagcgta atccaggcgg tttttacagc tataaccatt actgccggat gtaccgtgaa
     1381 tggctcaaaa ccacttcacc atcaatgcgt caggtacata aagctggcga aaaacttttc
     1441 gttgattact gtggacctac cgttggcgtt accgaccctg agaccggaga aataagaact
     1501 gctcaggtca tcgtagctgt tctcggggca tcaagttaca catgggcaga ggccacctgg
     1561 tctcagcagc ttgaagactg ggtgatgagt catgttcgct gcttccagtg gttgggtggc
     1621 gttcctgaac ttgttgttcc ggacaatctg aaaagcgcca catccagggc atgtaagtat
     1681 gatcctgacg ttaaccctac ctaccagcag atgcttgagc attataatgt cgcagttttg
     1741 cctgcgcggc cacgtaaacc gaaagataaa gccaaagctg aagttggcgt tcaggttgtt
     1801 gaacgctgga tcatggcccg aatcaggcat gagatcttct acagccttgc atcgcttaat
     1861 cagcgcattc gggagttgct ggaaagactg aataacaaaa taatgcagaa gttgggttat
     1921 tcacgtgcag aactcttcat ccagcttgat aaacccgcac tgaagcctct tcctgaagcc
     1981 agttacagtt acaccctggt gaagaaagtc agagttcatg ccgattacca cgtggaaatc
     2041 gacaaacatt actactcggt tccatgttcg ctgttaggcc agcaactgga agcatggatc
     2101 tccggagaac tggtaagact cttcaatcag gggcaggagg ttgctgtgca cccgcgcaag
     2161 cgtacttatg gctacagtac ccgcaacgag cacatgcctg aagctcatcg acagcatgcc
     2221 acctggacgc cagagcgtct tctggaatgg gcggggcaca taggcagtga aactcatagt
     2281 tatgtgcttc atatactgaa ctctcgtcca catccggaac aaagctatcg cttctgcctt
     2341 ggactcctga accttcataa aaaatacagt aaagccagac ttaatgcagc atgtgcaaga
     2401 gctctgaaaa caaaggtatg gcgtctgtca ggtattaaat cgatcctgga aaaaggtctg
     2461 gataaacaac ctgttcagga tccaaaacca gatctgttat ccacgatgga acacgaaaac
     2521 gtacgcggca gtgagtatta ccactgatac gggatccaat gatgaatcat ctttacgaac
     2581 aactgaccgc acttaaactc accggcttcc gtgatgcgct taaaaagcaa cttgctcagc
     2641 cgggcacata ccaggagctg ggcttcgaag aacgcctgtc attactgaca gcagaagaac
     2701 taacctgccg tgaaaacagg aaggcagagc gtctgatcaa acatgcacgg ttcagactta
     2761 atgctgagtt atcaaagctg gattatcgta acaatagagg gctggacagg gccctcatcc
     2821 gttcactcag tcagggaaac tggttaaccc tgaaacaaaa tattttactg accggggcca
     2881 ccggcagcgg taaaacgttc ctggcatgtg cacttggtca taatgcctgc cgacagggat
     2941 acaaggtcta ctattatcgc cttaaagcgc tgatggaaca gtgctatcag gggcatgctg
     3001 atggaagata cagcaaactt ttgaccaggc tgaataatag cgatctgctg cttctggatg
     3061 actgggggct ggaacctctc tcatcagaac agcgtagcga cctgctggaa atagtggatc
     3121 tgatgtacca acgaggctca atcatcgtag tgagccagtt gccggtggaa aactggtaca
     3181 aaatgatcgg agactccaca catgcggatg ccatcctaga tcgactggtt catggcagta
     3241 tcaagatcga acttaaagga gaatcaatgc ggaaaataca atctccgttg accgaaggag
     3301 atcagtgaag gtaatttaaa aacggttctg tgaaagtgac acgaaccgat ctccatcgat
     3361 gttactcacc gatctccttc acggtaatac gcaccaggct cccgcacagt ggctgagctg
     3421 gaatctgaag ttatgcaact gcgtaaggct ttaaatgaag cacgacttga gcgagatata
     3481 ttaaaaaaag caacagcgta ttttgcacag gagtcgctga aaaatacgcg ttagtcgaac
     3541 aatggcgaca acaattccct gttgaagcga tgtgtcgggt acctggtgta tccaggagcg
     3601 gttattacga ccggg
//
LOCUS       DAJTRD010000208         3562 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTRD010000208 DAJTRD010000000
VERSION     DAJTRD010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.209"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            119..1282
                     /locus_tag="OO315_004130"
     CDS             119..1282
                     /locus_tag="OO315_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5892803.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGIQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAESCRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            1631..2803
                     /locus_tag="OO315_004131"
     CDS             1631..2803
                     /locus_tag="OO315_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5892804.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2857..3480)
                     /locus_tag="OO315_004132"
     CDS             complement(2857..3480)
                     /locus_tag="OO315_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5892805.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAERPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT         1127 a          750 c          725 g          960 t
ORIGIN      
        1 atcccactaa gaataaatct ccttacctca caacacgaca agcctgcatt gcggctcttc
       61 agtctccgct gcatactgtc caggtgagcg cgggtgatgg cataacagag gaaagaaaat
      121 gtcactcttc cgcagaggtg aaatatggta cgccagtttc acattgccga acggtaaaag
      181 atttaaacag tctcttggaa caaaggacaa aaggcaggcg acagaactcc atgacaagct
      241 aaaggctgaa gcatggcggg tcagcaaact tggtgaaata cctgatataa cgttcgagga
      301 agcgtgtgtc aggtggcttg aagagaaagc acataaaaaa tcactggacg atgacaaaag
      361 ccggatcgga ttctggcttc aacatttcgc aggaatacaa ctaagagaca ttactgaatc
      421 aaaaatttat tcagcaatgc agaaaatgac gaaccggcgt catgaggaaa actggaaact
      481 cagggcagaa tcatgcagaa aaaaagggaa acctgttcca gaatacacgc caaaaccagc
      541 gtccgttgca acgaaggcta cgcatctttc atttataaag gccctactaa gagccgcaga
      601 gcgtgaatgg aaaatgctgg ataaggcacc aattattaaa gtgcctcaac caaagaataa
      661 acggatccgc tggctggagc cccatgaagc acaaaggctg attgatgaat gtccggagcc
      721 attaaagtct gttgttgaat ttgcactggc aacaggctta agacgctcga acatcatcaa
      781 ccttgaatgg caacaaatag atatgcagcg ccgggtggca tggataaacc cggaagagag
      841 taaatcaaac cgcgcaattg gcgttgcgct gaatgatact gcatgtcgcg tattgaaaaa
      901 acaaatcggg aatcatcacc gttgggtatt tgtgtacaag gaaagctgta ccaaaccaga
      961 cggaacgaaa gcgccaacgg tcaggaagat gcggtatgac gcaaacacag cctggaaagc
     1021 ggcgctgaga cgagcaggta ttgatgattt cagatttcac gacttgagac acacctgggc
     1081 aagttggctg gttcaagccg gagtcccgtt gtcagtgtta caggaaatgg gaggctggga
     1141 gtctatcgaa atggttcgtc gatatgctca ccttgcacct aatcacctta ccgaacacgc
     1201 acggcaaata gactcgatcc tgaacccatc ggtcccaaat tcgtcccagt caaaaaataa
     1261 ggaaggtact aatgatgtgt aacttattga tttaaatggt gccgataata ggagtcgaac
     1321 ctacgacctt cgcattacga atctgtagca ccaatcataa ctatctgttt tagcaagcat
     1381 taaccgcatt cactaagcta tagttgatga cacaaacaga aagttgatgc atgatgttgc
     1441 catatatatg tcacaaatac ggcacaacga tcttcaaaca tatagccaca cattcacaga
     1501 agagcacaaa gccttgcaat ccagtgcaaa gcttggtgtg tctccgtttt gtttcatcag
     1561 ttacacataa ctatatgatc attaactaaa agtcagtata cttggagaaa ttttaattgc
     1621 gtaatagagc atgcatatga ttgaaattaa cagtttatta ttaataacat ccgtgatatt
     1681 gatgtcgtta ttagctgtgg ggttatttga taaaatttcc ccaataaatc ttgttgagca
     1741 cggcaggaat aatcaaatag atggaatgcg ggggttctta gcaattttcg tgcttattca
     1801 tcacgcagca atttggaatg gatacttgtc atctggagta tgggaagcac cttcatcaaa
     1861 tctgttagca aatttaggcc aagttggtgt gtcattcttt tttatgatta ctggttatct
     1921 gttcttttca aagattatct ctggagatca ggactggaca aggctttatg tatcaagatt
     1981 actacgatta accccaatgt tcatagttag cctatgtctc atttttatca ttgtaggttt
     2041 taagtctgga tggagaatgc aggtatccac agaagagctt tttgtgtcaa taatgaagtg
     2101 gttgccattc actgcgctag gtatgccgaa cattaatgac gtaaaagatt catttactat
     2161 caatgccgct gtaacatgga cacttgtata tgaatggttc ttttattttt ctcttccggt
     2221 aatttccgcg ctcataaaaa gaaaagtcag tatttacatg gttatgatta gcgcaatatc
     2281 gctatttgtt ttcattttat ttttcagtaa aatacacata gcatcatttc tttttgggct
     2341 cctggcattt ttgctaaata aatcaaagat agtcaatgga attgccaaag caaaagtaac
     2401 accaattata ataactgcga taatggtttt tgaaatgacg tatttcaaaa caacttacgc
     2461 accgctacca ctgattcttt gtggaataac atttatcatt atcgcatcag gttgtgattt
     2521 gtatggaata ttaagattaa atataaccag aaagctagga gaaactacct acagcgttta
     2581 ccttctgcat gggatattcc tttattgcct aatgacgtgg attattccta ataattacac
     2641 tgaaaataca tttatcatac tggtaagcac aactgcattt cttattacgt tcacatcatg
     2701 cctaactttt aaattaatag agacgccatt catcaaatta acaaaacaaa ccacaacttt
     2761 agtaaaagaa ttaataccca cattaacaaa caacaatcaa taaacaataa acataagccg
     2821 ccctacatat atgtccaaat ggggcggcga atgtatttat attagtctgg taatgctaaa
     2881 tgcaaacgaa ctggagattc ctgccccgct aagcgtattg cgcagggtca ccctaaatgt
     2941 atctacgcca atataagtaa catcacaact aactgaatca ggcgcagata taggtgttat
     3001 ctgcacaata taaccgctag caactgctgc gagcggatga gaaaagctaa catcatacac
     3061 acctgtagaa accacgttaa ccgatgaaac accagtagca acgtcgatac taccattggc
     3121 gttaattttt cccgagcgtg tgactgacaa tgcagaagcc gatgttacct tactggttgt
     3181 ttctgtaaaa acacacccaa tgtcaactac ctcgcaatta gctcctgtta tccagtatgg
     3241 tcgttctgca cttggctgat aatcccctgc gctctggaac gttgtgccgt tgagtatgac
     3301 tgtaactttt cccccacctg agcttgttac ttgaatattc gtgtgcgtgt atcttgcact
     3361 gctcacgcga tgaaaatttc caccgtttat aaccacagtt acagggcgcg tcccagtatt
     3421 atctatagca aggtctgccc ccccggcatt agcctcaaag tatgggttgt ttaataccaa
     3481 agcgcacgaa ccgttaaggc catttatagc taattgcgca ccacctgtac tggaatcccc
     3541 ctgagtacca tttccttcac aa
//
LOCUS       DAJTRD010000209         3548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTRD010000209 DAJTRD010000000
VERSION     DAJTRD010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.210"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..465
                     /gene="pgrR"
                     /locus_tag="OO315_004133"
     CDS             <1..465
                     /gene="pgrR"
                     /locus_tag="OO315_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5892806.1"
                     /translation="RLGGEMDKDMIAIRIGPDIPMAIVGSPDYFSRRSAPTSVSQLID
                     HQAINLYLPTSGTANRWRLIRGGREVRVRMEGQLLLNTIDLIIDAAIDGHGLAYLPYD
                     QVERAIKEKKLIRVLDKFTPDLPGYHLYYPHRRHAGSAFSLFIDRLKYKGAV"
     gene            802..2415
                     /gene="mppA"
                     /locus_tag="OO315_004134"
     CDS             802..2415
                     /gene="mppA"
                     /locus_tag="OO315_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5892807.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="OO315_004135"
     CDS             complement(2466..3497)
                     /gene="ynaI"
                     /locus_tag="OO315_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5892808.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSISNYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1006 a          773 c          819 g          950 t
ORIGIN      
        1 cgtctgggtg gggagatgga taaagatatg atcgccattc gaatcgggcc agatatacca
       61 atggctattg ttggctcacc ggattatttt tctcgtcgaa gtgctccaac gtcagtgtca
      121 caattaatag atcatcaggc aattaatttg tatcttccca catcgggtac agcaaatcgc
      181 tggagattaa tacgcggtgg acgtgaagtt cgtgttcgca tggaaggtca gcttttactg
      241 aatacgatag acctgatcat tgatgctgca attgatgggc atggattggc gtatctacct
      301 tatgatcagg ttgagcgggc tattaaagaa aaaaaactga tacgtgtttt ggataaattc
      361 actccagatt tacccggtta tcacctgtac tatccacacc gtcgacatgc tggctcggca
      421 ttctcattat ttatagatag gctgaagtat aaaggtgctg tttagcacta cttgctgata
      481 cattaattta attcttctct taacgtattc tcagttcctt tcaacgtttt ggttattttt
      541 tattcttcgt acaatggcga cagatgctga ttatgatacc gaaaactggt ttgaacgtgc
      601 gaagcccgaa cgtagtgttc ggtgttctat gtgctttgcc acattttggc gtctattatt
      661 tacactaaat ctgatttgat atattgatac ttaaaacatt tgatgcttcc tttgtcactt
      721 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
      781 atgtcggtta gagggaaact tatgaagcac tctgtttcag ttacgtgttg tgcgctgttg
      841 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      901 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      961 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggtctggt gaatcagaac
     1021 gaaaaagggg agattgtccc cggcgtcgcg actcaatgga aaagtaatga caaccgtatc
     1081 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
     1141 gattttgtct acagctggca acgtctggtg gacccaaaaa cattgtcgcc atttgcatgg
     1201 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
     1261 gatcagcttg gcgtcaccgc agttgatgcc catactttga aaattcagct tgataaaccg
     1321 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
     1381 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
     1441 cttaaagagc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
     1501 aacgccaaaa cggtgctgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
     1561 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
     1621 cagaagctgt tgaaggatat tccggggcag gtttatacgc caccgcagct cgggacctat
     1681 tattatgcct ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1741 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tggggacggg cgaaaagcca
     1801 gcgtggcatt ttataccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1861 caaatgagtc aggaagaact gaatgcgcag gcaaaaactt tgttgagcgc agctggttat
     1921 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1981 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     2041 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgc
     2101 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaatg
     2161 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     2221 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     2281 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaaaccg
     2341 tggctgaaag gttatcccat taataaccct gaagatgtag cgtacagtcg gactatgtat
     2401 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     2461 ctgatttagc gcccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     2521 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     2581 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     2641 aatgacgagt cagcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     2701 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2761 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2821 acgctgatcg acgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2881 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2941 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     3001 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     3061 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag cactacaata
     3121 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     3181 atctgctgtt tttcaaactg attaatcagc agaaatagct taatcgtcag aataaccgca
     3241 atcagggacg tacagataaa atcgataacg cctggggtga taaatttgag tttatagttt
     3301 tctataacat aattactgat gctaccaaca gcactgataa ttatggtgta gattaaaaat
     3361 tgcaccgcat ggaataaaaa tccttttctt ttacgatttc cacggcgaaa ccaaaagctc
     3421 atcagaatca atgctgcgca gctaccgaaa ataatgacca gattaagcgc attatttgta
     3481 aacagttcag cgatcattgt tttatcaggc tcctccagat aattgtcgtc atgccggaaa
     3541 cccctggc
//
LOCUS       DAJTRD010000210         3456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTRD010000210 DAJTRD010000000
VERSION     DAJTRD010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.211"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(226..429)
                     /locus_tag="OO315_004136"
     CDS             complement(226..429)
                     /locus_tag="OO315_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892809.1"
                     /translation="MNETELKHVIALLLEDAKRLQQLEPNAGTGARIWLAKEALESGD
                     YDSEEAFYKAEGRAGYSPGLGGV"
     gene            complement(431..586)
                     /locus_tag="OO315_004137"
     CDS             complement(431..586)
                     /locus_tag="OO315_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5892810.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(789..1196)
                     /locus_tag="OO315_004138"
     CDS             complement(789..1196)
                     /locus_tag="OO315_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5892811.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1273..1500
                     /locus_tag="OO315_004139"
     CDS             1273..1500
                     /locus_tag="OO315_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5892812.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1484..1906
                     /locus_tag="OO315_004140"
     CDS             1484..1906
                     /locus_tag="OO315_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892813.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1984..2761
                     /locus_tag="OO315_004141"
                     /pseudo
     CDS             1984..2761
                     /locus_tag="OO315_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2768..3456
                     /locus_tag="OO315_004142"
                     /pseudo
     CDS             2768..3456
                     /locus_tag="OO315_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309972.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          885 a          764 c          940 g          867 t
ORIGIN      
        1 ccagaggatt acgaagggct tcataaatat atgctctcac taggattcag gagaacgatc
       61 ccccatggag atggttcgta taatcaactc ccggacggaa cttatgtttc cgaaaagggc
      121 ggtgatattt atcaaattcg cagccagata tctgactatg cagaccgact atccgggtat
      181 cgcgcgtctg tttttgtttg cgaattcagt caatgcgcat ggtatttata ccccgccaag
      241 acccggtgaa tatcctgcac ggccttctgc tttgtagaag gcttcttcgc tatcataatc
      301 gccagattcc agcgcttctt tagccagcca gatgcgtgcc ccagtgcctg catttggctc
      361 cagttgctgg aggcgttttg catcttctag gagtagagcg ataacgtgtt ttaattctgt
      421 ctcgttcatt ttactcacct gaatgtcttc ccaaccaacg acgtgcgcca gcttcggttt
      481 taaacgtttt gcttttggta tacgttatgg cggtgaatgt gccgtcctgg ttggggaaca
      541 cgccgtacac cggagattcg ttgttgccaa gatcgatagt attcatgttg accccatttc
      601 cccttaacgc cggggtagcg gaactgtttg ctgagaacac cgtgcggtgt cttgatgaat
      661 aaaatttaga ataacctaag gagggtgatc aagatttttg tgtagaaaaa cctaagtttt
      721 ttgatgtaaa aaacacaagt gtttgaaagt ttgtgctttt tattacaggg tgtggagaaa
      781 aaaggggatt atttgtttgc gcttcttttg cgagctttga gtagttcttc aaaaagtttg
      841 ttgaaatttt caactcgagc acgcatctct gacaacaggg ccttttgctc tgactcaggc
      901 agtgcgtcga acagttgaag taactctttt tgatcttctg tcagaatggc tggctgatta
      961 tccgggatcg gttcgcctgg ttgtttatct tcatccccaa aaagaagcca agtcggtgag
     1021 cactgaagcg cttggctcag tgcgaataat cttttccccg ccggctgtgt ttcatctctt
     1081 tcccattgag aaattgttac gtgagccact ttgaccagct tacctaatgc ggcctgagac
     1141 agttttaatt ttttacgcct atgtaagagg cgagcaccga aggtttcgtt tttcatatta
     1201 gggaattcta atttttcttg acttaggttt ctctacggcc tggtttcctt aggaaaatct
     1261 aaggagctcg atatgttgaa aattgatgct atagcgtttt ttggcagcaa aacaaagctt
     1321 gccaatgccg caggagttag gctggcaagc attgctgcat ggggggaact ggttcctgaa
     1381 ggtcgcgcga tgcgcctgca agaggcatct ggcggggaac ttcagtacga ccccaaagtt
     1441 tatgacgaat atcgtaaggc aaaacgacct gggaaggtga ttcatgaaaa tcaggcatga
     1501 gcacatcgaa tcagtgctgt tagccctggc agccgaaaaa gggcaggcgt gggtcgctaa
     1561 cgcaattact gaagaatatc tgcggcaggg gggcggcgaa ttgtctctgg taccaggcaa
     1621 ggactggaac aatcagcaga atatctatca ccgttggttg aaaggtgaaa cgaaagcgca
     1681 aagggaaaaa attcagaaac tgatccctgc ggttctggca attcttccgc gtgagctgcg
     1741 tcaccgactc tgtatcttcg ataccctgga acgccgtgca ttactggcgg cgcaggaagc
     1801 gttgagtacg gcaattgatg cgcatgatga tgctgtccag gccgtttacc ggaaagcaca
     1861 tttcagcggt ggtgggtcgc ccggcgattc tgtcgtagtg cattgattga aattaatcgt
     1921 gccggattgt tttgttcggt atcagttaaa tgtaacgctg cgagcgttac aaggtgaaaa
     1981 caaatggctt caaactggat aaagctcgag gttattacgc cggataagcc ggaaatattc
     2041 aggcttgctg agattctgaa tattgatcca gatgccgcat tagggaaggt tattcgcttc
     2101 tgggcatggg cggatcaaca aatgatagac ggtaatgcag attgtaacgc tcgcggcgtt
     2161 acaaaaagtg caatagatcg catcactttt atgtctggtt ttgctgatgc gttaattcag
     2221 gttggatggc tggtcgaaaa tgacggtggg ctttctctac ctaactttga acgtcataac
     2281 ggaaaaagct ctaaaaaacg ggcggttaca aacgagcgag taacaaaaat acgcgaactg
     2341 aaacgaaaag gtaacgctgc cagcgttaca caaacggatc aaaaagcgtt accagtggaa
     2401 gaggaagagg aagatctaaa tactgatctc cccctaaatc cccctcgcca aaaacgagcg
     2461 tctaaaaaat tcgagccgga ggctattgag ctgcccgatt ggttgccgga aacactctgg
     2521 catgagtggg tccggttcag acaggcattg cgaaaaccga ttcgaacgga gcagggcgat
     2581 acgggaactg gaaaaattcc gtcagcaggg ttttacacct gagcaggtga ttcgacacag
     2641 catcgccaat gaataccagg gcctgttcgc gccgaaaggt gttcggcctg agacgttgct
     2701 ccgacaggtt aacaccgtct cgttgccgga cagtgcgatc ccgccaggct tcagggggta
     2761 acggaccatg aaaaatattg cgacaggcgg cgttctggaa cgcatccgcc gactgacccc
     2821 gcaacatgta accgccccat tcagaacggt agcggagtgg cgcgagtggc aacttgctga
     2881 aggccagaaa cgtagcgagg agatcaaccg cctgaatcgc cagttgcggg tggaaaaaat
     2941 tctgaatcgc tcaggcatcc agccgttgca ccgtaaatgc tcgtttgcga attaccaggt
     3001 gcagaacgac ggccagcgat acgcgttaag ccaggcgaaa tccatcgccg atgaactgat
     3061 gaccgggtgt acaaattttg cgttcagcgg aaaacctgat accgggtaga atcacttagc
     3121 ggcagctatc gggaatcgcc tgctgaaaga cggtcagaca gtgattgtgg ttaccgtggc
     3181 tgatgttatg agcgccctgc acgccagcta tgacgacggg cagtcaggcg aaaaattttt
     3241 gcgggagctg tgcgaagtgg atctgctggt tcttgatgaa attggcattc agcgcgagac
     3301 gaaaaacgag caggtggtgc tgcaccagat tgttgatcgc cggacagcgt cgatgcgcag
     3361 cgtggggatg ctgacaaacc tgaactatga ggtgatgaaa acattgctcg gcgagcgggt
     3421 gatggatcgc atggtcatga acggcgggcg ctgggt
//
LOCUS       DAJTRD010000211         3433 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTRD010000211 DAJTRD010000000
VERSION     DAJTRD010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.212"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..332)
                     /gene="flhe"
                     /locus_tag="OO315_004143"
     CDS             complement(<1..332)
                     /gene="flhe"
                     /locus_tag="OO315_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5892814.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     ARQPASGLMTLVAWRYQLIGPTPAGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGG"
     gene            complement(332..2409)
                     /gene="flhA"
                     /locus_tag="OO315_004144"
                     /pseudo
     CDS             complement(332..2409)
                     /gene="flhA"
                     /locus_tag="OO315_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(2402..3433)
                     /gene="flhB"
                     /locus_tag="OO315_004145"
                     /pseudo
     CDS             complement(2402..3433)
                     /gene="flhB"
                     /locus_tag="OO315_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
BASE COUNT          837 a         1044 c          841 g          711 t
ORIGIN      
        1 ccgcctggca cttcccagat aaatcgcaac ggttctgctg ccgctatacc ggaaaaggcc
       61 acggtggtac cgctctgccc ctctaattcg acacaacgag attgcgaaca caagcgaacc
      121 cgcagtcctg caggtgtcgg gccgataagc tgataacgcc acgccaccag cgtcatcaat
      181 cctgaagcag gttgtcgcgc agaaagaggt gcagacgaca tcgactcacc gcgatgattt
      241 agcgtaattc cgacactact tgcctgccac atcccctccc cggcggcttg caccagcagc
      301 ggaaacaata atatcgctaa taaggttctc atcatttacc gccaattgtc gccgtcatgc
      361 ggatatgtcg gttatcagac agttccagat tcgacaggac cactaactgc ggcaagctgc
      421 ggcgcaggaa gcgagacaat aatggtcgca gcgcgtggtt caccaacaat actggcggcg
      481 cacccagcat ctcctgacgg gatagagctt cctgagtttg cgccagtaaa cgatccgcca
      541 gccctggctc cagtcctccc ccgccctgca gcgcctgtag taacaaacgt tccagcggtg
      601 tatcgaggcc aataacatgg acttcatctt tgccaggaaa ccactgctgg gtaatcgccc
      661 gtcccaacgc cacgcgcacg acggcgctta attcatgtgg atcgctttgg atgggcgcat
      721 gttccgccag cgtttcgaga atggtgcgca tatcgcgaat cggtactttt tcatcgagga
      781 gattttgcag cactttatgc agtgtggtga gcgtgacgac gccaggaacg agatcttccg
      841 tcagctttgg catctcctgg gcgacgcgat ccaacagctg ttgcgcctcc tgacgaccaa
      901 acagctctgc ggcatgctgg ctaatgaggt ggttaagatg cgttgctacc accgtgctgg
      961 cctcaaccac tgtgtacccc tgaatctgcg cctgttcttt tagcgcactt tcaatccaga
     1021 tagcattcag gccaaatgcc ggatcgacgg tcgcctcacc aggtaacgtc ccggcagcgg
     1081 ttccagggtt aatcgccagc cagcgccccg gataagcatc accactgcca atctccacgc
     1141 ctttcatcaa aatgcgatag cgggcaggtt gcagatccat attgtcgcga atgtgcacca
     1201 ctggcggcag aaatcccatc tcctgggcaa atttcttgcg gatactgcgt atacggccca
     1261 acaactcacc atcctgctgg aaatcgacca tcgggatcag tcgataaccc acttccattc
     1321 ccagagaatc ttccagttgt acatcgttcc acgtcgcttc gacaacggta ttattctctg
     1381 ccatttttac cggtttgggt tcggcaggcg ctttttgttc gcgtccgcgt atccaccagg
     1441 ccagccccag caatccggca gtgaacagca aaaataccag gttcggcatt ccaggcacca
     1501 ggccgagtaa accgagcacg gcggcgctta acaacataac gcttgggtta ctgaaaagct
     1561 gattcaccat ctgctcgcca acatcctgat cggtgctgac acgcgtaacg atgccccccg
     1621 cggcggtaga aatcaccagc gccggaattt gtgccaccag accgtcgcca atggtcaata
     1681 gcgtataact ttccgccgcg tgtcccatgc tcatgccagt tgcagcacgc cgaccagcaa
     1741 cccgccgaca acgttaatga ccatgatgag gatcccggca atggcatcgc cgcgaacaaa
     1801 cttacttgcc ccgtccattg agccgtaaaa atcggcttcc tgagtcactt cggagcggcg
     1861 ttttttcgcc tcatcttcac caatcaatcc agcgttaagg tcggcgtcaa tcgccatctg
     1921 cttacccggc ataccatcga gaacaaagcg cgcacccact tctgctatac gcccggcacc
     1981 tttggtaatg accataaagt tgatgatcac gagaatgaca aacaccacga taccgatagc
     2041 gaaattgcca ccaacgagga agtgaccgaa cgcttcgacc accttcccag ccgccgccgc
     2101 gccggtatgc ccttccatca aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa
     2161 caacatagta aacaacagaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa
     2221 catcgccacc agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag
     2281 tatgaatgcg ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc
     2341 ggcaaggatc tgccattatg tcgatttcag gtttgcgggc aggcgcagca tcgcggccag
     2401 attactcatg ggtcggtttc tcgttaataa aatccagggc tgccggcacc ggaagatgag
     2461 taggtgtaca gggcgctgtc caccagccag acgccagcgt ttcagttgcc agacccaggc
     2521 cagcacttcc gccaccgcgg cgtacagttg acccgggatt tgttgaccaa tctccgcatg
     2581 tcgatacagc gctcgcgcca gcggcggcgc ttcaagcgtc gggacgttat tttcagcgcc
     2641 aatttcacga atgcgcagcg cgaccagccc tgcaccttta gcgaccactt tcggtgcgct
     2701 cattttgttt tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac
     2761 atccgctttc ggcacatcgg ccatcatccg acgccgtgcg gcagctcgct gcatctgaca
     2821 gatccgtcct ttaacatggg ggtcgccttc gctttgtttg aactcatcac gaatatcctg
     2881 ccgcgacatt cgcagctttt tcaggtggct gaagatttgg aaaaagacgt caaatcccac
     2941 cattggaatg acaccaagca ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt
     3001 acccatggcg gtaatcggtg actcggccat taagcgcatc atctgcggcc agtgatgcca
     3061 gagaaaaaac cccgtcacgc tgccaaccag gatggttttc aaaattgcct taagcaactc
     3121 cgcgctagtc tgtgccgaga atatccgttt aatgcccggt agcgggttga gtttggaaaa
     3181 cttcggctgc aaggatttgc cgctaaatac cagccccccc agcataaccg gagaaatgag
     3241 cgccaccagc accacgccgc taatcagcgg tagcagcgcc agcatggctt ctctgatcag
     3301 cagaataatt tgcccgagga tcagattcgg gtctttgata atactgtgat caaaatgcag
     3361 cccagcggag agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac
     3421 actaacgccc acc
//
LOCUS       DAJTRD010000212         3414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTRD010000212 DAJTRD010000000
VERSION     DAJTRD010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.213"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..238
                     /locus_tag="OO315_004146"
     CDS             <1..238
                     /locus_tag="OO315_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5892815.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="OO315_004147"
     CDS             248..886
                     /locus_tag="OO315_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5892816.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="OO315_004148"
     CDS             889..2052
                     /locus_tag="OO315_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5892817.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3414
                     /locus_tag="OO315_004149"
     CDS             2136..>3414
                     /locus_tag="OO315_004149"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5892818.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEG"
BASE COUNT          760 a          803 c          987 g          864 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgcaaacgag
     3301 gtatgccgtt tgtggccgaa gcgatggagg tgctgggcgg cattggttat tgcgaggaga
     3361 gcgagctgcc gcggctttac cgggagatgc cggtaaacag tatttgggaa ggtt
//
LOCUS       DAJTRD010000213         3349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTRD010000213 DAJTRD010000000
VERSION     DAJTRD010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.214"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            19..897
                     /locus_tag="OO315_004150"
     CDS             19..897
                     /locus_tag="OO315_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5892819.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
     gene            894..2285
                     /locus_tag="OO315_004151"
     CDS             894..2285
                     /locus_tag="OO315_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5892820.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            2282..2539
                     /locus_tag="OO315_004152"
     CDS             2282..2539
                     /locus_tag="OO315_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892821.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            2634..2834
                     /locus_tag="OO315_004153"
     CDS             2634..2834
                     /locus_tag="OO315_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5892822.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            2827..3207
                     /locus_tag="OO315_004154"
     CDS             2827..3207
                     /locus_tag="OO315_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5892823.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
BASE COUNT          886 a          726 c          969 g          768 t
ORIGIN      
        1 cattctgagg tgacagcgat gatgacgttt aacctgcgtg aacaacaaaa aagactacag
       61 gcgcgaatgg atgagttacg ggcagagatt gcatttgctc agaagggcga aaagccatgg
      121 ccttatcgtt cctgcctgat gcgtgaaggt cgcggatatt gcgaaaaaca tggcgaatat
      181 cacacgcata tactggtgtg gagcgatcgt aatggcgagg acagagaaga aatttcatgc
      241 tgccctgact gcttaatcgc tgaggccaac gatttgacca tggagctgtc gtccatcaag
      301 gcggaagagc tgactgataa cgccggaatt gccctgcgtt ttcgggactg cgagtttgat
      361 aattatctgg aggttaatcc tggcgcagcc agaaatcttg cggcctgtcg ccgctatgcg
      421 gagaactggc cagatatgct ggagaacggt accagtcttg ttatgaccgg cagttgtggt
      481 accggaaaaa atcatctggc tgtggctatg gcaaaacaca tcatccgtaa ctatctggcc
      541 agtgtggaga tcaccgacgt gatgcgcctt acccgtgctg tgaaaaattg ctggcggaat
      601 gacagcgaaa aaacagcgga tgaagttatt gagcgttatg cgtcaatgga tttgctgatc
      661 atcgacgaag ttggcgttca gtttggcagc gcggctgaaa tggctatttt gcaggaaatt
      721 atcaacgcca ggtacgaaag tatcttgccc accattctga tcagtaacct ctcaccggaa
      781 gagttgtggg cgttcatcag tccccgaatt gccgacagga tcacagacgg gggacgcaac
      841 tggttgtcgt ttaactggcc cagttaccgt tctcgtatca gaggtgtggc tgcatgacaa
      901 ctccagtatg gcgtaacgat gaccttgaag gcgctgtcat cggcgcgttc tttctgcgcg
      961 gagcagatcc tgaagtgatg gatattctgg ccacactgcc agcggacgtt ttttctgtac
     1021 gagcgtatca ggatatctat acaggcatct gcagacaggc ccgtgtttca ggagtgattg
     1081 accccgtgct gttgtgtaat gagatgccgg aacttgcccc ggtgattact gataccgggc
     1141 gtaaaacctg ggtgaagtct tcactggagc actatgttgc agcgttgcgg cgcaatgccg
     1201 cactgcgcga tgcagaaaaa acactgaatg aggcgctgca gaaattacgt gatgcgcata
     1261 cctgtgaagc agctgaagat gccctgaagg atgcgcagaa catgatggtc acactgtcga
     1321 caggaaaggg cgtcattcag ccggtacata ttgatgatgt gcttccggaa gtggttgagc
     1381 gtgttgaatg ccggaatcag gggctggaga aatccaggac gttgatgacc ggtattgatg
     1441 aactggacgc aaaaacaggc ggtatggagc ccggagacct ggtattcatt gccgcccgtc
     1501 cttcgatggg gaaaaccgaa cttgcgctgg acatcatcga caaggtgact gagcaggggc
     1561 atggtgtgct tctgttcacc atggagatgg cgaacatcca gattggtgaa cgtatggtgt
     1621 ctgcggctgg agggatgcca gtatcacgcc tgaaatctgt ggctcacttt gaagatgaag
     1681 actggacgcg tttctcacaa ggggtggggc ggatgaccgg gcgcaatatc tggatggtgg
     1741 accaggcgaa cctggccatt gatgagatat gcgcaacaac gaaacaccac ctgattaaat
     1801 atccggaaac ggcgctggtg gtggttgatt atctcgggct gataaaaacc cgaaccacgg
     1861 ggcgtcatga ccttgccgtg ggtgaaatct caaaggggct taaaggcctg gcaaaatccg
     1921 gtggttttcc gttgattgcg ctgagccagc tctcccgcgg tgtggagtcc agacccaata
     1981 aacgtcccat gaactcagac ctgaaaaatt ccggagaaat agaggcggat gcagacatca
     2041 ttctgatgct ttacagggat gaagtgtaca acccggatac gcaggccagg ggcatcgcag
     2101 aaatcaatat cacgaaacaa cgtaacggtt ctctgggaac gatttaccgg cgtttttata
     2161 acggacattt tctgcctgtg gaccaggaaa gtgcacgggt gctttccact cccatgaagc
     2221 ccggcaatcc gcgcagatac agtaataaac gaactgacag cagtaagatg gagcgtttct
     2281 tttgaacaac cagacaatga cttttacccc tgaacaattg cgtaaacacg cgcaggaaat
     2341 gctgcgtcat gctgaacaac tcgaaaaaac gggcataaca aaagatgcta tccgtaaaga
     2401 tatggtaccc gcgcttcggg aactgatgca ggcgaagcat cgcgcacaga aagcagtaga
     2461 tgagctggtg gactgtgtgg cagagctgga aaccagagtc ggaaagtttg aaaaactggt
     2521 gcaggaggcg ctgcgctgat gcgccatgag tttgttttgc cctggcttgt gcgcgatcat
     2581 tatgtccctg gtggaaagcc cgggataaaa atcaccgaac cggagagtat ttgatgagcg
     2641 tcaaaattca aacgataccg gaactgctta tccagacacg aggtaatatg accgaagttt
     2701 cacgaatgct gaactgtaat cgcgccacgg taagaaaata tgctgaagat aaagagggca
     2761 aagggcacgc cattgttgat ggtgttctga ttgttcatcg tggatgggac aggggtaaag
     2821 acagtgatgc gtgatattca gcaggttctt gagcgctggg gcgcgtgggt ggctaataat
     2881 catgaggatg tgacctggtc gtccattgct gccggtttta agggattaat cccttcaaaa
     2941 gtaaaatccc gcccgcaatg ctgtgacgat gacgcgatgg ttatatgtgg gtgtatggcc
     3001 cgccttaacc ggaacaacaa cgatctacat gacttgctgg ttgactacta cgtgtttggg
     3061 atgacattca tgacgctggc gcggaagcat ggatgctcag atggttatat aggaaagaag
     3121 ctacaaaaag ccgagggggt ggtggaagga atgttgatga tgttgggtgt ccggttagaa
     3181 atggacaaat atattgaaca tccctgatgg taaggggcga ctttctagaa aatattttac
     3241 gtacgtaaaa aacgggatat tctgttaaga gtggttgctt cgccacacag cttaaacccg
     3301 cctccgggcg ggttttttat gcctgaaaaa cggtacagga cgttaaaca
//
LOCUS       DAJTRD010000214         3271 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTRD010000214 DAJTRD010000000
VERSION     DAJTRD010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.215"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..2215)
                     /locus_tag="OO315_004155"
     CDS             complement(<1..2215)
                     /locus_tag="OO315_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5892824.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGV"
     gene            complement(2549..>3271)
                     /locus_tag="OO315_004156"
     CDS             complement(2549..>3271)
                     /locus_tag="OO315_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5892825.1"
                     /translation="SLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGV
                     GESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVT
                     QADKTEPCHEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLV
                     SALMTALPDHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRT
                     ALRAVRDTVVSVARAVWNWIFF"
BASE COUNT          642 a          969 c          885 g          775 t
ORIGIN      
        1 ttacaccggt ctgatggctg cgggagcctg ccagaatccg gtcatagtcc attgcctgtg
       61 tcagcatgga ggcatacagg gggacttctg cacgataagc attttcactg cgcagatacc
      121 agctctcatc actgtcccgg ttgagggagt agttaaaggc accggcctgc agcttattcc
      181 cctggataaa ggccccttcc tccgtggtgg caccgttaat ggcttccacc acctgaatac
      241 ccttaccgct ggtcgccagc cccgacgcac tgttgccggc gttcaccagg ttcaggatgg
      301 tttttccggt tgccctgcca ccgtcaatga ccagtctgtc agcattgttc tgtgccatat
      361 ccgggcgtac acgcaggctg atggtgccat tctgtccgtt caggtttttc actttcaggg
      421 ttgccggtac gaacttccct gtgcgggtgg aggtgaaatg aatctgtccg gcatggctga
      481 ggtcatccac caccgactgc accgtggcgt tatcgggaat attccaggtg gcaccagagg
      541 cgagagtgac attcgtgggg tcaatggcac cgttcagcac agtgctgccg gtcagcttca
      601 gggctgtacc gcgctgagca atgacatccg tggtgacggt actgtcgttc agcgtcagcg
      661 tgccttcatt caggttgacg gctttctggg tgagtgtggt gttgctgaca gtgagtgtgc
      721 cacttcctga tttttccacg ctgccgttac cggtgagagc gcctccctgc aggagtgcat
      781 cgccttcacg gatggtcagg gtatcgttgt tcaccgtctt cccggaaagg gtaagtatgg
      841 cgccgttatt cagcgtggtg gtgcccgcca gtgtgccgcc cctggcggtg aacagtccgc
      901 catttaccgt ggtatccgtg gccgtatcac cggcgttcag cgtgaatgaa ctgccttttt
      961 ccagcatcag ggcatccgcc tgaccgcctc cgatactgaa tgcctttccg tcgctccggg
     1021 taccactgac agcggcaccg gaatcggcca gcagtacacc gccatttccc atggatacgg
     1081 tggtggcggt gccaccgttg cggacatcca gcgttccgcc atcatccacg cgggtattag
     1141 tggctgtgtg tccggtcagc acatccaggc gtccgccatt ttccagtacg acattatcag
     1201 ctttaccctc cacaacagag aatgctccca ggcggtttgt gccggtgacg gttgcagctg
     1261 tactggtaac cagtgcaccg cccgtgttct gggtgacatc agacgcttta ccgccggcat
     1321 tcacctgcag ctttcctttc tggttgatgg tggtatgcgc ggcagttcct ccttccttaa
     1381 tcacctgcca gccatcacgg tttatcagcg tctctgttgc cgtgccaccg ttgtgtacat
     1441 actggttacc cccctccagt cgggtgttat gtgcctctcc gtgtaccgtc tggtcaccac
     1501 cggcgtaaat gagggtgtcc cgtgccatcc ccgaagacca gataacctgc cggccatttt
     1561 tattgatggt ggtggattca gccgtccctc cgaccatctg accgctggac acattttcag
     1621 actccggacc accttctgca ccggtgttga cgatggttcc ggttgccagt ccgccatgtt
     1681 ttatggtctg gtaaccatcc tggttaataa ttgtaccggc agctttccct cccccgtgta
     1741 cccactgctc acctctgtta tccagcgtgg tgttcaccgc cagtccgtta aggctctgcc
     1801 ctccgccatc acgaataacc gtatcactgg cagttcctcc tgcctgcaca atctgacgac
     1861 catttgcggt gacagtggtg tttctgcctg tgccacccgc ttttatccat tgcccgccgg
     1921 tgttttcgtc actgtccggc cccagctcaa gcccggtact gacagtcacg ccatcagctg
     1981 ttccggatac aaactggttg tcatggttta ccagtgttcc gccattcact gtttcacccg
     2041 ggtgcacaac gatgtcagca gccagcaccg ggagtgacgt gaccgcggca agagacagtg
     2101 caaccgccac accgccacgt ttaccctgtg cgcgggccag ttcggaggca accacgaaag
     2161 cgcccgtaat gtgattccat accagcctgt agcaggtatt cagatgtcgt ttcatcagca
     2221 tttccttaat cagagtgagg gtgaataaaa aagccggtat ccacagatgt ggataccggc
     2281 aggtaaaggg ttacagtgcc ggaggtccgg ctgacggaag tttctgacgt gtggctctgt
     2341 tgttccctga atctcatgtc tgtgtgtatc tgcgcacgtt gctaacagga gttattatta
     2401 gcataacgat cgatattaac gatcggtaaa ataggtatcg atcgttttat tctattatca
     2461 gtctattgca cggacaagag agacagtaac gaaaacagcg cttttgtgag cagactcaga
     2521 ggcagggagg acatcatcca caacaggttc agaagaagat ccagttccat accgcgcggg
     2581 caacagagac cacggtgtca cgaacagcgc gcagggccgt acggacaaca gaggcaacac
     2641 agacgctctc cgctgtgtca aatatccggt ccaccgcacc ggtaaactgt tcacgggcct
     2701 gagagcggac agactccgtg cgcagctcgt cctgcagtcg ggtcatcagg gggctggcgg
     2761 catggtcggg aagcgctgtc atgagcgcac tgaccagcgt atccagttcc cagccggtgc
     2821 gggccgatac ggcaacaacc ggatgtacgg gccggaacag acggaatacc gcctccgttt
     2881 tctcacgaat gttctgttcc tgtgcgggag aaggctgaat acctgccata tcccattcat
     2941 ggcagggttc cgttttgtca gcctgcgtca ccacaaacag cacctgctga tgtccgcact
     3001 gcaggatgtg tcgccagaaa tactcatcca cagacagggc ccggtcatcg gctttaatca
     3061 gccacagcac caggtccagt tcaggcagaa tgtcacggta cagggcttca tactctgcat
     3121 ccctgtcccg gctctcgccc accccgggca ggtcagtgat aaccatgctg tgaccgtgac
     3181 cactcagacg gaagcgctgc acttcccggg tgccggcgtg aacatcactg accggtgtga
     3241 cctccccctg aaacagtgca ttacagagtg a
//
LOCUS       DAJTRD010000215         3259 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTRD010000215 DAJTRD010000000
VERSION     DAJTRD010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.216"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..442
                     /locus_tag="OO315_004157"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO315_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1027..1743
                     /locus_tag="OO315_004158"
     CDS             1027..1743
                     /locus_tag="OO315_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892826.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2295..2699
                     /locus_tag="OO315_004159"
     CDS             2295..2699
                     /locus_tag="OO315_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892827.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2762..3259
                     /locus_tag="OO315_004160"
                     /pseudo
     CDS             2762..3259
                     /locus_tag="OO315_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          909 a          675 c          750 g          925 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaatactgg attgtgggcc ataaatattc caaaaagata
      481 gcccgctccg tttattaccg gaacgggcga gagcatgaca ttgtctgtta ttataatgag
      541 actgctgatt ctacttattt ttaatttcaa gaaaagcgtt ttctacaaag aattatgaga
      601 tttaatgttt tgtttagaca atattatcat tctgttcaat aatgaaagtc ttcatcaaca
      661 acaagtaaaa acaacgtccc tctcatcgaa ccggacttgc agatttcccg catccggctc
      721 tcttgcaaca catcagacct tcacgttcgg gcggttatgg ggaaggtcca gcaagctgta
      781 tgagccctaa ctgtttgaat accagtagat taccagaaac ttctatcgat tgaagacatt
      841 accaactagc gttactaaat gttgaccaag ttttgttcac ttgtctggtt ttgatagtag
      901 cattctgcag agtattttga tatgataaat atgcattaac aatgatatta ttgttaagtt
      961 cctagcatga aaattaaaag atcaactttt atctcgaata ttttttacat tatcagttag
     1021 tttcttatga atgacaattc attgcttaga aattcttcat tattcatcgc ctatatggga
     1081 tgtgtagggt gggtcagtgc ttattcttat ggctggggaa cctcttttta ctatggattt
     1141 ccatggtggg ttgttggtgc aggtttggat gatgttgcca gaagtttact ctatgctatc
     1201 atagtaatgg gtatactttt taccggttgg gggattggta ttttattttt tcttcttata
     1261 aagaaaagaa gtaaaataca agacttgagt ttttttagac tattttttgc tattacccta
     1321 ctgtttttcc ctgtaatatt cgaactttta attctcaaac aatatttcat acttccatta
     1381 tcactttcat tcattattag ttcattggtt atttcaataa taattcgtat atatggacgg
     1441 atcttttctg tatcatgttt ttcagatatc ccttttgtta gagaacaccg cattaagtta
     1501 attatggctg gttttttggt ttatttttgg tttttttcct ttcttgttgg ctggtataaa
     1561 cctcaattaa aaaaagaata tcagatgctt tgttataata atagttggta ttatgttctt
     1621 gctcgttatg attcacggct cgttttatct agttcattta aagatgacag taacagattc
     1681 ttaatattta atacagaaca gtcgggattt tatgaaatca atgatgttta tgtcagaaag
     1741 taataatcct ctcccacaaa gtgaattaaa tgaatatata atgcgttcga agtatatgta
     1801 ttgagctgtc atactgagaa acaaacctta gggcagcaca attaaagcca gaaacaacta
     1861 tgcaagaaag ttactaaata agtgctcgga acagtaaaac actcaggggg gactcagtcc
     1921 ggattatgta atcagataaa ttgccaaaca tcacaaatca ccagccggac tgagtgatgc
     1981 cgagcattgc accaataccc cgtgatgaac gatgcctgat gcagaaagct ccctataaaa
     2041 caaagaacaa aaactacgac cggcgattca ccgtcatact gatgctgcat cgtggtgaca
     2101 ctgtctgtca ctttcatgtt gactgaacaa ctctgctgcg aagaaggtgt accgccggcg
     2161 ggatttgatt gtccggcgtc tagcttccac ttccctgatg gaactgaaag ttgttatggc
     2221 cggtcagctt gccctgtccg caggacatga ggtgctgtga cggacagcac cagtaacgga
     2281 aagtgacaat gaaaatgaat actgcgctga tagttgcact aatgtgtatg tggtatgcgg
     2341 tacccgctgc tgcaaaagag actttactgg ctatgccacg taattcaaca gaacattgct
     2401 atgcggagat taatgtgcac ggaccatatg gggtgtattt ccgagtggtc ccgcatcccc
     2461 ccgggggaaa gagctgggtc gaatgtaaca gtgactacta ttactctgat aaaccaccgg
     2521 gagtacagat actcggaacc cgtgcagggt gtcgggtata cggaatctgc gggaccacct
     2581 cgacactgca tgtggctgga agaggggttg tgtgtattaa aaatatatgc agtccacgtg
     2641 ggatgataat tcacaggata cggaaacgtc ctgtagtggc tgtaagcgat gagatgtgat
     2701 cttacccagc aatagtagac acgcggctaa gtgagtaaac tctcagtcag aggtgactca
     2761 catgacaaaa acagtatcaa ccagcaaaaa aacacgtaaa cagcattcgc ctgaattttg
     2821 cagtgaagcc ctgaagcttg ctgaacgcat cggtgttgct gccgcagccc gtgaactcag
     2881 cctgtatgaa tctcagctct atgcgtggcg cagcaaactg cagcaacaga tgacctcgtc
     2941 tgagcgtgaa agtgaactgc ccgcctgaag cggcaactgg cggagcaggc agaggaactg
     3001 gctatcctcc aaaaggccgc gacatacttt gcgcagcggc tggtatacgt ggtgtcagcg
     3061 tcggacagga ataagccctc gtcagcagtt ccgtcagcac tgcgacagcg ttgtcctcgc
     3121 ggcggccttt acccggtcaa aacagcgtta cggtgcacca cgcctgacgg atgaactgcg
     3181 tgctcagggt taccacttta acgtaaaaac cgtggcggca agcctgcgcc gtcaggggct
     3241 gagggcaaag gcctcccgg
//
LOCUS       DAJTRD010000216         3179 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTRD010000216 DAJTRD010000000
VERSION     DAJTRD010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.217"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO315_004161"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO315_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5892828.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO315_004162"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO315_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          774 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggaccc tgagcaggtg gtaattgtaa gacgttgcgg
     2821 gcgacggtat tacaattgca catcctgtcc tgtttcttta ggacgtatca tggcacagca
     2881 tatcaactga taaataatcc ctgtgcagtg cagccgctgt tgcataaaat ttcgctctgc
     2941 gtcgagtgtt tcttaatcaa taattccgct gatactccgg aaaaacgcga gtctgttctc
     3001 atgcctgaaa tgcccacacc ccacgcaaaa acaagttttt gctgattttt ctttataaat
     3061 agagagttat gacaaattag ttcttcttgc tctctttgtg atatttaaaa aagcggtgtc
     3121 ggcgcggtgt tgtagctgcg ccaacaccgc tttttagggg tggtactgac tattttcat
//
LOCUS       DAJTRD010000217         3114 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTRD010000217 DAJTRD010000000
VERSION     DAJTRD010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.218"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO315_004163"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO315_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5892829.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="OO315_004164"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="OO315_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO315_004165"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO315_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5892830.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO315_004166"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO315_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5892831.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3114)
                     /locus_tag="OO315_004167"
                     /pseudo
     CDS             complement(2163..3114)
                     /locus_tag="OO315_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          754 c          758 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gggc
//
LOCUS       DAJTRD010000218         3071 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTRD010000218 DAJTRD010000000
VERSION     DAJTRD010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.219"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..500
                     /locus_tag="OO315_004168"
     CDS             <1..500
                     /locus_tag="OO315_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5892832.1"
                     /translation="DGEILKSACEINDSDKKIEVALGHYNAEQFRSVGDRSPKIPFTI
                     PLVNCPVTGWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTGVGIRID
                     DAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEADAVVNV
                     TLDYR"
     gene            580..1275
                     /locus_tag="OO315_004169"
     CDS             580..1275
                     /locus_tag="OO315_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5892833.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1304..>3071
                     /locus_tag="OO315_004170"
     CDS             1304..>3071
                     /locus_tag="OO315_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5892834.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          888 a          718 c          684 g          781 t
ORIGIN      
        1 ttgatggcga gattttaaaa tccgcttgtg aaatcaatga ctctgataag aaaattgaag
       61 ttgctcttgg tcactataat gccgaacagt ttcgttctgt tggcgatcgt agcccaaaaa
      121 ttccattcac tattccttta gttaactgcc cagtgactgg gtgggagcac gataacggca
      181 acgttgaagc gtctttccgt ttatggctgg aaacgcggga taatggcacc gtccctaact
      241 tccctaatct ggcaaaagtg ggctcttttg ctggcacagc gacaacaggt gtgggtatcc
      301 gtattgacga tgcggaaagc ggaaacctta tgccactgaa tgctatgggg aatgataaca
      361 cgatttatca gatcccagcg gactccaatg gtattgtcaa tgttgacctc atcgcttact
      421 acgtatcaac cgttgaagca tcagaaatca ccccagggga agcagacgct gtcgttaacg
      481 taacgctgga ttaccgttaa tacgttacgg cgttatctga cctttcagat aacgcccttt
      541 tccttcctct ttctcgttgt atcaggttga aaaataacta tgtcaaaacg aacattcgcg
      601 gtgatattaa ccttgttgtg tagcttctgt atcggccagg cgcttgcagg gggtatcgtt
      661 ttacagcgaa cgcgagtgat ctatgatgcc agccgcaaag aggctgcgtt acctgtcgca
      721 aacaaaggcg cagaaacgcc ttatttactg caatcatggg tagataatat agatggtaaa
      781 agccgtgccc catttattat aaccccaccg ctatttcgtc ttgaggctag cgatgactca
      841 tcactgcgaa ttattaaaac agctgataac ctgcctgaaa ataaagagtc gctgttctac
      901 attaatgttc gtgccattcc agcaaagaaa aaatcagatg atgttaatgc taacgagttg
      961 acgctggtat ttaaaacgcg ggtcaaaatg ttttatcgcc ccgcacacct gaagggacgg
     1021 gtaaacgatg cgtggaaatc actggaattt aaacgtagtg accattcact caatatatat
     1081 aacccaactg aatattacgt cgtatttgcc ggactggcag tcgataaaac cgatctcaca
     1141 agcaaaattg aatatatcgc gcccggagaa cataaacagt taccacttcc agcatctggc
     1201 ggaaagaacg tgaaatgggc tgcgatcaac gattatggcg gcagttccgg gacagaaact
     1261 cgtccactgc aataaaaaat ataaaaacac aggccatcag ggaatgccac aacgacacca
     1321 ccagggacat aaacgcacac cgaaacagtt ggcgctcatt atcaaacgtt gtttgccgat
     1381 ggtgctcact ggcagcggca tgctttgcac taccgctaac gccgaagagt attatttcga
     1441 ccccattatg ctggaaacta caaaaagtgg tatgcaaaca accgatctgt cacgtttttc
     1501 aaaaaaatac gcacaactac caggaactta tcaggttgat atctggctga ataaaaagaa
     1561 ggtttcacag aaaaaaatta catttaccgc caatgcagag caacttctgc agccacagtt
     1621 tacggtagaa caactacgtg agctgggtat taaggtggat gaaatcccgg cgctggctga
     1681 aaaagatgac gatagcgtga tcaactcgct tgaacaaatc attcccggta cagctgctga
     1741 atttgatttc aatcatcagc gacttaattt gagcattccc caaattgcac tgtaccgtga
     1801 tgcaagaggt tacgtctccc cttctcgttg ggacgatggt ataccaacgc tgtttaccaa
     1861 ctactcgttt acaggttctg ataaccgtta ccgccagggc aatcgtagcc aacgacagta
     1921 cctgaatatg caaaatggtg ctaattttgg cccctggcga ttacgtaact attctacgtg
     1981 gacacgcaac gatcaggcgt caagctggaa cactatcagt agttatttac aacgtgatat
     2041 caaggcgttg aagtctcagt tgcttctggg agaaagcgcc accagcggca gtattttttc
     2101 cagctacgcc tttactggcg tgcaactcgc ttccgacgat aatatgttgc caaacagcca
     2161 gcgcggattt gccccaacgg tacgcggtat cgcaaacagt agtgcaatcg tgactatcag
     2221 gcaaaatggt tatgtgatct atcaaagcaa cgtgccagcg ggtgcctttg aaattaacga
     2281 tctctacccc tcttccaaca gcggcgattt agaagtcacg attgaagaaa gtgacggtac
     2341 acaacgtcgc tttatccagc cttattcttc attacccatg atgcagcgac ctgggcatct
     2401 aaaatatagc gcgaccgctg gacgctatcg cgctgatgca aacagtgata gcaaggaacc
     2461 cgaatttgct gaagccacgg caatatatgg tttgaataat acttttacgc tgtatggcgg
     2521 cctgctcggt tctgaagatt attatgcgct ggggatcggt atcggcggca cacttggcgc
     2581 actgggcgcg ttgtcgatgg atatcaacag agctgacacc caattcgata accagcactc
     2641 ttttcatggc tatcaatggc gtactcagta catcaaagat atcccggaaa ccaacaccaa
     2701 tatcgctgtt agctactatc gctataccaa cgatggctat tttagttttg atgaagccaa
     2761 tacccgcaat tggggctata acagtcgcca aaaaagtgaa attcaattca acatcagcca
     2821 gacaatattt gatggggtaa gtctgtatgc ctccggttca cagcaagact attggggcaa
     2881 taacgagaaa aacagtaata tctctgttgg ggtttccggc cagcaatggg gaattggtta
     2941 cagcctgaat tatcaataca gccgctacac tgatgaaaat aatgaccgtg cactctcttt
     3001 gaatctcagt attccgttag aacgctggtg gatttgcccc tatatttcca gacatctgtt
     3061 atcacttaac c
//
LOCUS       DAJTRD010000219         3033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTRD010000219 DAJTRD010000000
VERSION     DAJTRD010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.220"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..723
                     /locus_tag="OO315_004171"
     CDS             <1..723
                     /locus_tag="OO315_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5892835.1"
                     /translation="SLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGV
                     GESRDRDAEYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVT
                     QADKTEPCHEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLV
                     SALMTALPDHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVART
                     VLRAVRDTVVSVARAVWNWIFF"
     gene            1095..>3033
                     /gene="ag43"
                     /locus_tag="OO315_004172"
     CDS             1095..>3033
                     /gene="ag43"
                     /locus_tag="OO315_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5892836.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGV"
BASE COUNT          709 a          772 c          908 g          644 t
ORIGIN      
        1 tcactctgta atgcactgtt tcagggggag gtcaccccgg tcagtgatgt tcacgccggc
       61 acccgggaag tgcagcgttt ccgtctgagt ggccatggtc acagcatggt tatcactgac
      121 ctgcccgggg tgggcgagag ccgggacaga gatgcagagt atgaagccct gtaccgtgac
      181 attctgcctg aacttgacct ggtactgtgg ctgattaaag ccgatgaccg tgccctgtct
      241 gtggatgagt atttctggcg acacatcctg caccggggac atcagcaggt gctgtttgtg
      301 gtgacgcagg ccgacaaaac ggagccctgc catgaatggg atatggccgg tattcagcct
      361 tctcccgcac aggcacagaa tattcgcgaa aaaacggagg cggtattccg tctgttccgg
      421 cccgtacatc cggttgttgc cgtatcggcc cgcaccggct gggaactgga tacgctggtc
      481 agtgcgctaa tgacagcact tcccgaccat gccgccagtc ccctgatgac ccgcctgcag
      541 gacgagctgc gcacggaatc tgtccggggt caggcccgtg aacagtttac cggtgcggtg
      601 gaccggatat ttgacacggc ggagagcgtc tgtgttgcct ctgttgcccg tacggttctg
      661 cgtgccgtcc gtgacacggt ggtctctgtt gcccgcgctg tatggaactg gattttcttc
      721 tgaacagtct gccagggaaa tgcctgataa cattacgtta accaactgaa aacatgacac
      781 aaacccctca tactcctcct gtttctcttg cgtgaccgcc ttgtcgttaa tagaataaaa
      841 ctatcgataa aacagatatc gatcgttgct atcgatcgtt tatagtggtt aatggtctgc
      901 gaaaaggcag ctgaatctct tcatcatgca gaacggaatt gcacacaacg gactgatact
      961 tctctggcgg atgagagagg ggaagcattt atgtccggga aaaaccactt tatctgacca
     1021 tgctgttttg tacctgccgg tatccacttt tgtgggtacc ggctttttta ttcaccctct
     1081 gtaaggaaaa gctgatgaaa cgacatctga acaccagcta caggctggta tggaatcaca
     1141 ttacgggcac cctggtggtg gcttccgaac tggcccgctc acggggaaaa cgcgccggtg
     1201 tggcggttgt gctgtctctt gctgctgtca catcagtccc ggcactggct gctgacacga
     1261 ttgtacaggc gggagaaacc gtgaacggcg gaacactgac aaatcatgac aaccagattg
     1321 ttctcggtac ggccaacgga atgaccatca gtagcggtct ggagtatggg ccggataacg
     1381 aggccaatac cggcgggcaa tggatacaaa atggcggtat cgccaacaac actactgtca
     1441 ccggtggtgg tcttcagaga gtgaatgccg gaggaagcgt ttcagacacg gttatcagtg
     1501 ccggaggcgg acagagcctt caggggcagg cagtgaatac cactctgaac ggcggtgagc
     1561 agtgggtaca tgaaggcggg attgcaacgg gtaccgtcat taatgagaag ggctggcaga
     1621 ccgtcaaatc cggcgcaatg gcaaccgaca cggttgtgaa taccggcgcg gaagggggac
     1681 cagatgcaga aaatggtgat accgggcagt ttgttcgcgg aaatgccgta cgtaccacta
     1741 tcaatgaaaa tggtcgtcag attgtggctg ctgaaggaac agcaaatacc actgtggttt
     1801 atgccggcgg cgaccagacg gtacacgggc atgcgctgga taccacactg aatggcggtt
     1861 accagtatgt gcacaacggc ggtacggcgt ctggcactgt tgtaaacagt gacggctggc
     1921 agattatcaa ggaaggtggt ctggcggatt tcaccaccgt taaccagaaa ggcaaactgc
     1981 aggtgaacgc cggtggtacc gccacacatg tcaccctgaa acagggtgga gcactggtta
     2041 ccagtactgc ggcaacggtt cttggcagca accgtctggg caatttcacg gtggaaaacg
     2101 gtaaggctga cggagttgtt ctggagtccg gcggtcgcct ggatgtactg gaaggccatt
     2161 cagcgcagaa aacacgggtg gatgacggcg gaaccctggc agtgtctgcc ggtggtaagg
     2221 caacaggtgt caccatgaca tccggtggtg ccctgattgc agacagtggt gccactgttg
     2281 aggggaccaa tgccagcggt aagttcagta ttgatggcac atccggtcag gccagcggac
     2341 tgctgctgga aaatggcggc agctttacgg ttaatgccgg aggactggcc agcaacacca
     2401 ctgtcggaca tcgtggaaca ctgacgctgg ctgccggggg aagtctgagt ggcagaacac
     2461 agctcagtaa aggtgccagc atggtactga atggcgatgt ggtcagtacc ggcgatattg
     2521 ttaacgcagg ggagattcgc tttgataatc agacgacacc ggatgccgcg ctgagccgtg
     2581 ctgttgcaaa aggcgactcc ccggtaacgt tccataaact gaccaccagt aacctcaccg
     2641 gtcagggtgg caccatcaat atgcgtgttc gccttgatgg cagcaatacc tctgaccagc
     2701 tggtgattaa tggtggtcag gcaaccggca aaacctggct tgcgtttaca aatgtcggaa
     2761 acagtaacct cggggtggca acctccggac agggtatccg ggttgtggat gcacagaatg
     2821 gcgccaccac agaagaaggt gcgtttgccc tgagtcgccc gcttcaggcc ggcgccttta
     2881 actacaccct gaaccgtgac agcgatgaag actggtacct gcgcagtgaa aatgcttatc
     2941 gtgctgaagt ccccctgtat acatccatgc tgacacaggc aatggactat gaccggattc
     3001 tggcaggctc ccgcagccat cagaccggtg taa
//
LOCUS       DAJTRD010000220         3009 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTRD010000220 DAJTRD010000000
VERSION     DAJTRD010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.221"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..142
                     /locus_tag="OO315_004173"
     CDS             <1..142
                     /locus_tag="OO315_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892837.1"
                     /translation="IYGLIVPALISMKDTVAVISGFALACLTPPCIYAIYKGLSFSKD
                     KR"
     gene            139..966
                     /locus_tag="OO315_004174"
     CDS             139..966
                     /locus_tag="OO315_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5892838.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1010..1759
                     /locus_tag="OO315_004175"
     CDS             1010..1759
                     /locus_tag="OO315_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5892839.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            1756..2325
                     /locus_tag="OO315_004176"
     CDS             1756..2325
                     /locus_tag="OO315_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892840.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2449..2691
                     /locus_tag="OO315_004177"
     CDS             2449..2691
                     /locus_tag="OO315_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5892841.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
     gene            2695..2841
                     /locus_tag="OO315_004178"
     CDS             2695..2841
                     /locus_tag="OO315_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892842.1"
                     /translation="MNKAFELWVHQRYGNHYDLTRDVDGFYCREIVKRMFEVWCHCRG
                     LSVV"
     gene            2850..>3009
                     /gene="xisR"
                     /locus_tag="OO315_004179"
     CDS             2850..>3009
                     /gene="xisR"
                     /locus_tag="OO315_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HCR5892843.1"
                     /translation="MGNVIQLAPNEWVCESVLIAITGLKPGTILRTRKECWMVGREYI
                     HVSPDGNPK"
BASE COUNT          810 a          725 c          790 g          684 t
ORIGIN      
        1 tatttacgga ttaatcgttc cggccctcat atcaatgaag gatacggtag cagtgatttc
       61 tggctttgct ctggcgtgtc tgaccccgcc ctgcatttat gccatttata agggtctttc
      121 tttttctaag gataaaagat gaaaaaagtt attcctgctt tagcccttgt tctgctgacc
      181 accggacttg tcggttgcga tcgcgttgag cctggtaatg ttggcatcaa ggtaaacaaa
      241 ctgggcgacg ataaaggcgt cggagaagtg gtcggtgttg ggcgctactg gacgggatgg
      301 aacactgagg tttacatctt cccgaccttc aaacaaatga agacctacga tgagccattc
      361 agcttccaga tgagtgacgg tacaaccatc ggctatcaca tcggcgtggc ctacaaagtt
      421 gatccatcca aagttaccac agtctttcag acctaccgca aaggcgtgga cgacattacc
      481 gacaccgacc tgcgccagaa gatagccgac gcactcaacc gactggccag caaaatgacc
      541 accgacaaat ttatcgacgg cggcaagtct gagctgctgg atgcagctct taaagacatt
      601 caggaagaaa tgacgcccat cggtattcag gtaatgagcc tctcatatgt gggtaagccg
      661 gagtacccgc ctactgttat cgacagcatt aatgccaaag tcacggcgaa ccagaaaacc
      721 ctgcaatgcg agcaggaagt aaaacagcgc gaagcggaag ccaacatgtt gcgcgcggaa
      781 gctgccggac aggcagatgc gattcgcaca aaagcccagg ccgaagccga tgctattcgt
      841 ttacgcggtg aagctctgcg ccagaaccca aacgttatgg agctggaagc catcaacaag
      901 tggaacggta cactgccgca atacatgacc agcggtgcca acacaccatt tattcaggtt
      961 aagtaataca tatgcccggt attacacgcc ggggtgtctg gagataaaaa tgaatactgt
     1021 aaccatcaac aacaaacagt ttccggtaat cgaatatcgc ggtcagcgtg ttgtgacgct
     1081 ggcgatgatt gatgaagtcc accagcgacc ggaaggaacc gcgcgtgcag cattcaaccg
     1141 aaaccgtgag catttcatca gcggtgtgga ttatgccgaa ttaggtgcgg acgtaatacg
     1201 tacggacctc ccggaaggaa cattctctaa atttgcacca tcggggattg tactttttga
     1261 atcaggttac ctgatgttga cgaagccatt caatgacgcc cttgcctggc aggttcagcg
     1321 cgaactggtt aacagctact tccgcactcg cacaccgcta acggaaatcg agatgatcgc
     1381 tgcgatagcc gccgacgctg ttcgtcaaca gaagcgcctg aatcatgtgg aagaacagct
     1441 cgaaacagtc acagaagcgg tggaaaccat caaacgcgga aatatgcgcg ccggatatgt
     1501 cggttaccgc caggtggtag ccaaaagtgg aatgagtgac accaagtgtc ggaatctggt
     1561 gaacgcatat caaattccta cagacaccca cgaattcatg actccggacg gtctgttgtc
     1621 tcgcagggct atcgtcgagc ttgagccatt tatggcggcg ttccaccaaa tgatgtcaga
     1681 ggctgaccca cgcggcacac gctggtatca cccgaagatg gggcttttcc aggtgatcgg
     1741 atgggaggat aaagcatgat catccagtca aaacttattc gcgccgctct ggtgtgcgct
     1801 gctaaaaacg acgttcgtta ttacctgaac ggtcttcaca tcacgccgaa acatattgag
     1861 gcaaccaatg gttccgtggc actgcgcatg gctcacggca tccggacgaa gaaaaacatc
     1921 attgtccagt tcgaaggggg cgtcccggcc aaagccgaaa cgacagagct gatttttagt
     1981 aaagagccga tcgctgttca tcgcgaccag tttcagcgac gactgtccat taccggcatt
     2041 aaactggtgg acggttgttt tccggatttg gatcgcatca ttccgaaaaa gtttgaccgc
     2101 tgtactcacc cggtgctcca ggcgggttac ctgagttatc cagagaagat gtttggtcgt
     2161 gagcgtaaat ttattcccgt ccagttacgt ccctccggtg acgggcaagc ggtcagaatt
     2221 cagtttgatt ccatcatcaa ctcaatgtat ggcaatcctg aatttgttgt gatgccttgt
     2281 cgtgatcatg gcgatttcaa cgtggctcag gagcatccgg aatgaaaatc gaataccagg
     2341 acgccgcagg aggtgaatca atgagctggc ctgatgcaat cgtaactctg gggatggtat
     2401 tcgcagtagc gtttgttgtg tactcgattt gtcgatgggg ataaccacat gttcgctttg
     2461 attcaacgcg ggcagatata cacagacaga gccggatacc cagtagaaat tactcgcagt
     2521 actgagcact cagtgttctt tcggaggatg gacggacgta ccgggcgagt acgcatcggt
     2581 gaattcagta gcctgttcga gcatattgac cacctggaat atcacaagat tctggctgaa
     2641 acagaacaag aaaagcacct gaagaaatta cgcgcaatgc aaaggaagta aaaaatgaat
     2701 aaagcgtttg agctatgggt ccaccagcgt tacggcaacc actacgacct gacgcgagat
     2761 gttgacggtt tctactgtcg tgaaattgtg aaacgaatgt ttgaggtctg gtgccactgc
     2821 cgtgggctga gtgttgtgtg aggtaatgca tgggcaatgt gattcaactg gctcccaatg
     2881 aatgggtttg tgaaagcgtt ctaatcgcaa ttaccgggct caaaccaggc acaattcttc
     2941 ggacccggaa agaatgctgg atggttggaa gagagtatat tcacgtatca ccagacggta
     3001 atccaaagc
//
LOCUS       DAJTRD010000221         2953 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTRD010000221 DAJTRD010000000
VERSION     DAJTRD010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.222"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            143..2011
                     /gene="kup"
                     /locus_tag="OO315_004180"
     CDS             143..2011
                     /gene="kup"
                     /locus_tag="OO315_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5892844.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2178..2597
                     /gene="rbsD"
                     /locus_tag="OO315_004181"
     CDS             2178..2597
                     /gene="rbsD"
                     /locus_tag="OO315_004181"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5892845.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2605..>2953
                     /locus_tag="OO315_004182"
     CDS             2605..>2953
                     /locus_tag="OO315_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5892846.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          659 a          702 c          779 g          813 t
ORIGIN      
        1 caaagggggg gctttttctt tgagccaggt taatgtggcc gcatttagga gtacgatttt
       61 gccgttaatc gtgcatactg tgcgcttttt tgtgggccaa gggactaagc acacatttca
      121 tatttcaacg aaagactagt ctatgagcac tgataataag caatcattgc ccgcgattac
      181 cctcgcggcg attggagttg tctacggcga tattggtacc agcccgttat atacacttcg
      241 tgaatgtttg tccggccagt ttggttttgg cgttgaacgc gatgccgtgt ttggcttttt
      301 atcgctgatc ttctggctgc taatctttgt ggtttccatt aaatatctca ccttcgtgat
      361 gcgggcagat aacgccggtg agggggggat cctgacgttg atgtcgcttg ccgggcgtaa
      421 tacgtcggcg cgaaccacat caatgctggt gattatgggg ctaatcggcg gcagcttttt
      481 ctatggtgaa gtcgtcataa cacccgctat ttcggtgatg tcagccattg aaggtctgga
      541 aatcgtcgcc ccgcagctgg atacctggat agttcccctc tcaattatcg ttctcacttt
      601 attatttatg attcaaaagc atggcacggc tatggtcggt aagctgtttg cgccgatcat
      661 gctgacctgg tttttgattc tggcaggact ggggttacgt agcattattg ctaacccgga
      721 agtgctgcat gcgctgaatc cgatgtgggc ggtgcatttc ttccttgaat acaaaacggt
      781 ttcttttatt gcattagggg cagtggtgct gtcgattacg gggggcgagg tgctgtatgc
      841 tgatatgggg cactttggta agttctctat tcgcctggcg tggttcaccg tcgtattacc
      901 ttccttgacc cttaattact tcggtcaggg agcgttgttg ttaaagaacc cggaagcgat
      961 taagaacccg ttcttcctgt tggcaccgga ctgggcgctg atcccactgc tgatcatcgc
     1021 cgcactggcg acggtaattg cctcgcaggc ggttatctct ggcgtcttct cattgacgcg
     1081 tcaggcggta cgtctgggat atttgtcgcc gatgcgcatt attcacacct ccgaaatgga
     1141 gtcagggcaa atctatattc cgtttgtgaa ctggatgctc tatgtcgcgg tcgtgattgt
     1201 gattgtcagc tttgagcact ccagcaacct ggcggcggcg tacgggattg cggtgaccgg
     1261 aaccatggta ctgacgtcta ttctctcgac taccgtggca cgtcagaact ggcactggaa
     1321 taagtatttt gttgcgctga tcctgattgc tttcctttgt gtcgatattc cattgttcac
     1381 cgctaacctc gataaactgc tctccggcgg ctggttgcca ttgagcctcg gtactgtgat
     1441 gtttatcgtg atgaccacct ggaagagcga gcgtttccgc ttgctgcggc ggatgcatga
     1501 acatggtaac tctctggaag cgatgattgc ttcgctggag aaatcaccgc ctgttcgcgt
     1561 gcccgggacc gcggtgtata tgtcgcgtgc aatcaacgtc attccctttg cgctgatgca
     1621 taaccttaaa cataacaagg tattgcatga gcgggtgatt ctgttaactc tgcgcaccga
     1681 agacgcgcca tatgtccata acgtccgtcg ggtacagatt gaacaactgt cgcccacttt
     1741 ctggcgcgtg gtggcaagtt atggttggcg agaaacgcca aacgtggaag aagttttcca
     1801 ccgctgcggt ctggaaggat taagttgccg gatgatggaa acctccttct ttatgtcgca
     1861 tgagtcgttg atcctcggca aacgcccgtg gtatttgcgt ctgcgcggca agctgtactt
     1921 gctgctgcaa cgtaatgcgc tgcgtgcacc agatcaattt gaaatcccgc caaacagggt
     1981 tatcgaactg ggtactcagg tcgaaatcta acgccagacg cctcctttct tcataagggg
     2041 gcgtttttgt tttcatggtt aatcaccatg taaaacgttt cgaggttgat cacatttccg
     2101 taacgtcacg atggttttcc caactcagtc aggattaagc tgcggatcag cgaaacgttt
     2161 cgctgatgga gaaaaaaatg aaaaaaggca ccgttcttaa ttctgatatt tcatcggtga
     2221 tctcccgtct gggacatacc gatacgctgg tggtgtgtga tgctggttta cccatcccca
     2281 aaagtacaac gcgtatcgat atggcattaa cccaaggtgt accttctttt atgcaggtgc
     2341 tgggcgtcgt cacaaatgaa atgcaggtcg aggctgccat tatcgcggaa gagatcaaac
     2401 aacataatcc gcaactccac gaaacgttgc tcactcacct tgagcagctg caaaaacacc
     2461 agggaaatac cattgaaatt cgttacacca cgcatgaaca attcaaacaa caaaccgcag
     2521 aaagtcaggc ggtaattcgc agcggagaat gttctccgta tgcgaatatc attctctgtg
     2581 ctggcgtgac gttctgaggc cgtcatggaa gcattacttc agcttaaagg catcgataaa
     2641 gccttcccgg gcgtaaaagc cctctcgggc gcagcgttaa atgtctatcc gggccgtgtg
     2701 atggcgctgg tgggcgaaaa cggcgcgggt aaatccacca tgatgaaagt gcttactggt
     2761 atctatactc gcgatgccgg cacgctttta tggctgggga aagaaacgac atttaccggg
     2821 ccgaaatctt cccaggaagc cgggattggg attatccatc aggaactgaa cctgatcccg
     2881 cagttgacca ttgccgaaaa cattttcctc ggtcgtgagt ttgttaatcg ctttggcaaa
     2941 attgactgga aaa
//
LOCUS       DAJTRD010000222         2939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTRD010000222 DAJTRD010000000
VERSION     DAJTRD010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.223"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..2743
                     /locus_tag="OO315_004183"
     rRNA            <1..2743
                     /locus_tag="OO315_004183"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            2839..>2939
                     /gene="rrf"
                     /locus_tag="OO315_004184"
     rRNA            2839..>2939
                     /gene="rrf"
                     /locus_tag="OO315_004184"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          771 a          651 c          923 g          594 t
ORIGIN      
        1 tccataggtt aatgaggcga accgggggaa ctgaaacatc taagtacccc gaggaaaaga
       61 aatcaaccga gattccccca gtagcggcga gcgaacgggg agcagcccag agcctgaatc
      121 agtgtgtgtg ttagtggaag cgtctggaaa ggcgtgcgat acagggtgac agccccgtac
      181 acaaaaatgc acatactgtg agctcgatga gtagggcggg acacgtggta tcctgtctga
      241 atatgggggg accatcctcc aaggctaaat actcctgact gaccgatagt gaaccagtac
      301 cgtgagggaa aggcgaaaag aaccccggcg aggggagtga aaaagaacct gaaaccgtgt
      361 acgtacaagc agtgggagca cgcttaggcg tgtgactgcg taccttttgt ataatgggtc
      421 agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga aaccgagtct
      481 taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc catgggcagg
      541 ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa aattagcgga
      601 tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt ctccccgaaa
      661 gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt tcggcaaggg
      721 ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt tatcacggga
      781 gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac cgccagctaa
      841 ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag ccaggatgtt
      901 ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga gtcggcctgc
      961 gcggaagatg taacggggct aaaccatgca ccgaagctgc ggcagcgacg cttatgcgtt
     1021 gttgggtagg ggagcgttct gtaagcctgt gaaggtgtgc tgtgaggtat gctggaggta
     1081 tcagaagtgc gaatgctgac ataagtaacg ataaagcggg tgaaaagccc gctcgccgga
     1141 agaccaaggg ttcctgtcca acgttaatcg gggcagggtg agtcgacccc taaggcgagg
     1201 ccgaaaggcg tagtcgatgg gaaacaggtt aatattcctg tacttggtgt tactgcgaag
     1261 gggggacgga gaaggctatg ttggccgggc gacggttgtc ccggtttaag cgtgtaggct
     1321 ggttttccag gcaaatccgg aaaatcaagg ccgaggcgtg atgacgaggc actacggtgc
     1381 tgaagcaaca aatgccctgc ttccaggaaa agcctctaag catcaggtaa catcaaatcg
     1441 taccccaaac cgacacaggt ggtcaggtag agaataccaa ggcgcttgag agaactcggg
     1501 tgaaggaact aggcaaaatg gtgccgtaac ttcgggagaa ggcacgctga tacgtaggtg
     1561 aagcgacttg ctcgtggagc tgaaatcagt cgaagatacc agctggctgc aactgtttat
     1621 taaaaacaca gcactgtgca aacacgaaag tggacgtata cggtgtgacg cctgcccggt
     1681 gccggaaggt taattgatgg ggttagcgca agcgaagctc ttgatcgaag ccccggtaaa
     1741 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
     1801 ctgcacgaat ggcgtaatga tggccaggct gtctccaccc gagactcagt gaaattgaac
     1861 tcgctgtgaa gatgcagtgt acccgcggca agacggaaag accccgtgaa cctttactat
     1921 agcttgacac tgaacattga gccttgatgt gtaggatagg tgggaggctt tgaagtgtgg
     1981 acgccagtct gcatggagcc gaccttgaaa taccaccctt taatgtttga tgttctaacg
     2041 tggacccgtg atccgggttg cggacagtgt ctggtgggta gtttgactgg ggcggtctcc
     2101 tcctaaagag taacggagga gcacgaaggt tggctaatcc tggtcggaca tcaggaggtt
     2161 agtgcaatgg cataagccag cttgactgcg agcgtgacgg cgcgagcagg tgcgaaagca
     2221 ggtcatagtg atccggtggt tctgaatgga agggccatcg ctcaacggat aaaaggtact
     2281 ccggggataa caggctgata ccgcccaaga gttcatatcg acggcggtgt ttggcacctc
     2341 gatgtcggct catcacatcc tggggctgaa gtaggtccca agggtatggc tgttcgccat
     2401 ttaaagtggt acgcgagctg ggtttagaac gtcgtgagac agttcggtcc ctatctgccg
     2461 tgggcgctgg agaactgagg ggggctgctc ctagtacgag aggaccggag tggacgcatc
     2521 actggtgttc gggttgtcat gccaatggca ctgcccggta gctaaatgcg gaagagataa
     2581 gtgctgaaag catctaagca cgaaacttgc cccgagatga gttctccctg accctttaag
     2641 ggtcctgaag gaacgttgaa gacgacgacg ttgataggcc gggtgtgtaa gcgcagcgat
     2701 gcgttgagct aaccggtact aatgaaccgt gaggcttaac cttacaacgc cgaagatgtt
     2761 ttggcggatt gagagaagat tttcagcctg atacagatta aatcagaacg cagaagcggt
     2821 ctgataaaac agaattagcc tggcggccgt agcgcggtgg tcccacctga ccccatgccg
     2881 aactcagaag tgaaacgccg tagcgccgat ggtagtgtgg ggtctcccca tgcgagagt
//
LOCUS       DAJTRD010000223         2891 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTRD010000223 DAJTRD010000000
VERSION     DAJTRD010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.224"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..782
                     /gene="lsrF"
                     /locus_tag="OO315_004185"
     CDS             <1..782
                     /gene="lsrF"
                     /locus_tag="OO315_004185"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5892847.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            806..1096
                     /gene="lsrG"
                     /locus_tag="OO315_004186"
     CDS             806..1096
                     /gene="lsrG"
                     /locus_tag="OO315_004186"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5892848.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1136..1894
                     /gene="tam"
                     /locus_tag="OO315_004187"
     CDS             1136..1894
                     /gene="tam"
                     /locus_tag="OO315_004187"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5892849.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1898..2812)
                     /locus_tag="OO315_004188"
     CDS             complement(1898..2812)
                     /locus_tag="OO315_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HCR5892850.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTGDLQRVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
BASE COUNT          760 a          667 c          773 g          691 t
ORIGIN      
        1 tggattgggg aatgcagtca cgcttatcgt ggatatttaa tccgaaaacg ggtaaaactg
       61 tgatgctggc ctttgaccat ggttattttc aggggccgac tatcggactt gaacgcattg
      121 atataaatat cgccccgctg tttgaacatg ccgatgtatt aatgtgtacg cgcggcattt
      181 tgcgcagcgt agttcccccg gcgaccaata agccggtggt actgcgggcg tcaggtgcga
      241 actctattct ggcggaatta agtaatgaag ccgtggcgtt atcgatggat gatgccgtgc
      301 gcctgaacag ttgcgcggtg gcggcgcagg tttatatcgg cagcgaatat gaacatcagt
      361 cgatcaaaaa tattattcag ctggtcgatg ccggaatgaa agtgggaatg ccgaccatgg
      421 ccgtgactgg cgtgggcaaa gatatggtgc gcgatcagcg ttatttctcg ctcgcgactc
      481 gaatcgccgc tgaaatgggg gcgcaaatta tcaaaaccta ttatgtcgaa aaaggttttg
      541 aacggattgt tgccggatgt ccggtaccca ttgttattgc tggcggtaaa aaattaccgg
      601 agcgcgagac gctggaaatg tgctggcagg ctatcgatca gggcgcttct ggtgtggata
      661 tggggcgtaa tattttccag tctgaccatc cggtggcgat gatgaaagcc gtacaggcgg
      721 tggttcacca taacgaaacg gctgatcggg catatgaact ctatctgagt gaaaaacagt
      781 aactgcggat ttaaggagaa gaattatgca cgtcacactg gttgaaatta acgttcatga
      841 agacaaggtt gacgagttta tcgaagtttt tcgccagaac cacctgggct ctgtacagga
      901 agaaggtaat ttgcgcttcg atgtcttaca ggacccggaa gtgaattcgc gcttttatat
      961 ttacgaagcc tataaagatg aagacacagt ggcattccat aaaaccacgc ctcactacaa
     1021 aacctgtgtc gcgaaactgg aatctttaat gaccgggccg cgtaaaaaac gtctgttcaa
     1081 tgggttgatg ccgtgaggct acaattgggg taacgcgctg acgggagtaa aaaaaatgtc
     1141 tgactggaac ccctctttat atctgcactt ttccgctgaa cgatcgcgtc cggcggtgga
     1201 gctgcttgcc agagtgccgc tggagaatgt cgaatatgtt gccgatcttg gctgtggccc
     1261 aggtaacagc accgccctgc tgcatcaacg ttggcctgcg gccaggataa caggcatcga
     1321 ctcgtctccg gcgatgattg ctgaagcgcg cagtgctttg ccagactgcc agttcgtgga
     1381 agcagacatc cgcaactggc aaccggaaca ggcactcgat ctgatttttg ctaatgcctc
     1441 actgcaatgg ctgcccgacc actacgaatt gtttcctcac ttggtttctt tacttaatcc
     1501 gcagggcgtg ctggcagtac agatgccaga taactggctg gagccgaccc atgtgctcat
     1561 gcgcgaagtt gcttgggaac aaaactaccc ggatcgcggg cgtgagccgt tggctggcgt
     1621 tcatgcttac tacgatattt tgagcgaagc cggatgtgag gtcgatatct ggcgaaccac
     1681 ttactatcac cagatgccgt cgcaccaggc gattatcgat tgggtgaccg ccactggatt
     1741 acgtccgtgg ttacaggatc tgaccgagag cgaacagcag ctttttctta cgcgctacca
     1801 tcagatgctg gaagagcagt atccactgca agagaacgga cagatactgc tggcatttcc
     1861 gcgtctgttt attgttgccc gccgtacgga gtaattatca cgtcagctgg taatgacgat
     1921 cgggaagaat ttttgctgga atttctgctt catcgttcat ctgtaacagg tcaatttcaa
     1981 tagcgttgca gatggcatcc agcggtaaat cattgttttc agtaccgaac ggatcttcca
     2041 gttcttccgc cagacaatcc agcgaaataa aagtgtagga aatcagcaca gagataaaag
     2101 gcgtcatgta atgcaggtcc acgaccagcg cgaacggcag catgatacaa aacagataaa
     2161 cggtacgatg caaaatcagg gtgtaggcaa agggaattgg cgtgtaggca atgcgctcac
     2221 agcctgccag aaccgctgaa atatcattaa gccgatcgtt gaggctaata aacaggatat
     2281 ctgaaagctg tccattgcgg cgctgaaccg ccaaccattc tcccattatt aacaagatac
     2341 ggttagctgg agagttcgaa gccagtacac gctgaagatc tccagtcttg agataatgag
     2401 ccagcacttc cgcctgtggc tgtttgcgta atgtcatgcg taaacagtgg gcgaaggcga
     2461 tttgcagcct ggcaaactcc cttacacttg ccgaatccgg caatgtcgtt tttacctctc
     2521 gcagtaacga ccgtgaggca atcatcaact gcccccaaag ttttcgcgct tcaacgtaac
     2581 gggcgtaccc ggcattatta cgaaaaccaa gaaaaatggc gatggcgaca ccgagaatgc
     2641 tgaacggtgc gagggtgaat ttgatgccca gatgcgtgta ccagggcagc atgaatataa
     2701 cagcaataga aaagagaaaa ttgagtagta agcgcgagga tatcttggat aatactgagc
     2761 cgtgccagac aaaaatacgg cgcagccagt gttgttgtgg acgaacaatc atggttatct
     2821 tcaggcgtgg aaaagtcgcc ctattaaacg tgattacgat cacattctca agacgctact
     2881 tacaaattac c
//
LOCUS       DAJTRD010000224         2807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTRD010000224 DAJTRD010000000
VERSION     DAJTRD010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.225"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            6..81
                     /locus_tag="OO315_004189"
     tRNA            6..81
                     /locus_tag="OO315_004189"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="OO315_004190"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="OO315_004190"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="OO315_004191"
     CDS             complement(299..1057)
                     /locus_tag="OO315_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5892851.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="OO315_004192"
     CDS             complement(1065..2168)
                     /locus_tag="OO315_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5892852.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..>2807)
                     /locus_tag="OO315_004193"
     CDS             complement(2178..>2807)
                     /locus_tag="OO315_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5892853.1"
                     /translation="FILAVVMAIVLSVGLFRFNKTYQIKTGQLRRSWPIAAVLIIGLP
                     LLAQWLFGAALHWDVPALQGFNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQA
                     VPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDM
                     VSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          763 a          739 c          675 g          630 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gttgcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccctcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgt agcttgttcc actatctccc cacgatccat aaaaattacc cggtcagcga
      421 cggtacgtgc aaaccccatc tcatgtgtta cacacaacat tgtcataccc gactgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggtt
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc tgaaatctgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttcttagg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt aaaacggtca gatgagggaa gagattgaaa tgctgaaata
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctacgac aatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccggt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt atggacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgttgg ctataacgtg acatgctgaa acaaaagatc
     1141 cagtagatca gcgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaagc cgatgatgat cactaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca acgccagcga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccacctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatact ggtgccttct
     1501 gccataaaca acggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaatgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctcaagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccaggccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgatcg atacaagccc aatcagtaat
     1861 gcgaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgaa tgaagaccca acaggcaccg gcttttgtgc agtcagcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gccgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaataacgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatta ctcgcagtgc ctggggaata atgacctggc gtagcgtaac cgggttgggt
     2461 aaccccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agaggtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccattcc gccgcggaag ttaaagcctt gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggagcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaa
//
LOCUS       DAJTRD010000225         2807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTRD010000225 DAJTRD010000000
VERSION     DAJTRD010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.226"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..525
                     /gene="ssuE"
                     /locus_tag="OO315_004194"
     CDS             <1..525
                     /gene="ssuE"
                     /locus_tag="OO315_004194"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5892854.1"
                     /translation="SLLEYAREKLNGLDVEVYHWNLQNFAPEDLLYARFDSPALKTFT
                     EQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVVLPLATGGTVAHLLAVDY
                     ALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTHLDTALETFWQALHRRDV
                     QVPDLLSLRGNAHA"
     gene            518..1477
                     /gene="ssuA"
                     /locus_tag="OO315_004195"
     CDS             518..1477
                     /gene="ssuA"
                     /locus_tag="OO315_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5892855.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1474..2619
                     /gene="ssuD"
                     /locus_tag="OO315_004196"
     CDS             1474..2619
                     /gene="ssuD"
                     /locus_tag="OO315_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HCR5892856.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAY
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2628..>2807
                     /locus_tag="OO315_004197"
     CDS             2628..>2807
                     /locus_tag="OO315_004197"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter"
                     /protein_id="HCR5892857.1"
                     /translation="MMATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPE
                     GVVTAFWTLSASGELW"
BASE COUNT          625 a          770 c          779 g          633 t
ORIGIN      
        1 tccttgctgg aatatgcgcg ggaaaaacta aatggcctag atgtagaggt ttatcactgg
       61 aatctgcaaa acttcgcccc ggaagatctg ctttatgctc gtttcgatag tccggcactc
      121 aagaccttca ccgaacagct gcaacaggct gatgggctga ttgtcgccac gcctgtgtat
      181 aaagccgcct attccggcgc gctgaaaacc ctgctcgacc tgctgccaga gcgcgctttg
      241 caaggcaaag tggtgctacc gctggcgacg ggcggtaccg tggcccatct gctggcggtc
      301 gattatgccc ttaaaccagt tttaagcgca ctgaaagctc aggagatcct gcacggcgtg
      361 tttgccgatg actcacaatt aattgattac catcacagac cccagttcac gccaaatctg
      421 caaacccatc ttgataccgc gctagaaact ttctggcagg cattgcaccg ccgcgatgtt
      481 caggttcctg accttctgtc tctgcgaggt aatgcccatg cgtaacatca ttaaactggc
      541 gctggcggga ttgcttagcg tctctacttt tgcggttgct gcagaatcct cgcctgaagc
      601 gttacgtata ggctatcaga aaggcagtat tggtatggtg ctggcaaaaa gccaccagtt
      661 actggaaaaa cgctatccgc aatcaaaaat ctcctgggtg gagttccccg cgggtccgca
      721 aatgttggaa gcgttaaacg ttggcagtat tgatctcggc agtaccgggg atattccgcc
      781 aatctttgcc caggctgccg gggctgattt ggtatatgtg ggcgtcgagc caccgaagcc
      841 caaagccgaa gtgattctgg tggcagaaaa cagcccgatc aaaaccgtag ccgatcttaa
      901 aggtcacaaa gttgcctttc agaaaggttc cagttcacac aaccttttac tgcgtgcact
      961 gcgtcaggcc ggacttaaat tcaccgatat ccagcccact tacctgacgc ctgccgatgc
     1021 ccgcgccgcg ttccagcaag gtaacgttga cgcctgggct atctgggatc cctactactc
     1081 cgctgcgtta ttacagggcg gcgtgcgggt gctgaaagac ggcaccgatc tcaatcaaac
     1141 tggatcgttt tatctggcag ctcgtcccta tgcagaaaaa aacggcgctt ttattcaggg
     1201 cgtactggca acctttagtg aggccgatgc gttaacccgc agccagcgcg aacaaagcat
     1261 cgctttactg gcaaaaacga tgggcttacc ggcaccggtg attgcctctt acttagatca
     1321 tcgccctcct accaccatca aaccggttaa cgccgaggtt gccgccttac agcagcaaac
     1381 ggcagatctg ttttatgaaa accgtctggt gccgaaaaaa gtcgatattc gccagcgcat
     1441 ctggcagccc actcaactgg aaggaaaaca attatgagtc tgaatatgtt ctggttttta
     1501 ccgacccacg gtgacgggca ctatctggga acggaagaag gttcacgccc ggttgatcac
     1561 ggttatctgc aacaaattgc gcaagcggcg gatcgtctgg gctataccgg tgtgctgatc
     1621 ccaacggggc gatcgtgtga agatgcgtgg ctggttgcag cgtcgatgat cccggtgacg
     1681 cagcggctga agtttcttgt cgccctgcgt cccagcgtaa cctcacctac tgttgccgcc
     1741 cgccaggccg ccacgcttga ccgtctctca aatggtcgtg cgttgtttaa tctggtcaca
     1801 ggcagcgatc cacaagagct ggcaggcgac ggcgtgttcc ttgatcatag cgagcgctac
     1861 gaagcctcgg cggaatttac ccaggtctgg cggcgcttat tgctggggga aaccgtcaat
     1921 ttcaacggta aacatattca tgtgcgcgga gcaaaactgc tcttcccgcc gattcaacag
     1981 ccttatcctc cgctttactt tggcggatcg tcagatgtcg cccaggagct ggcggcagaa
     2041 caagttgatc tctacctcac ctggggcgaa ccgccggaac ttgttaaaga gaaaatcgaa
     2101 caagtgcggg cgaaagctgc cgcgtatggg cgcaaaattc gtttcggtat tcgtctgcat
     2161 gtgattgttc gtgaaactaa cgacgaagcg tggcaggccg ccgagcggtt aatctcgcat
     2221 cttgatgatg agactatcgc caaagcacaa gccgcattcg cccggacgga ttccgtagga
     2281 caacagcgaa tggcggcgct acataacggc aagcgcgaca atctggagat cagccccaat
     2341 ttatgggcgg gcgttggctt agtgcgcggc ggtgccggaa cggcgctggt gggcgatggt
     2401 cctacggtcg ccgcgcgaat caacgaatac gccgcgcttg gcatcgacag ctttgtgctt
     2461 tcgggctacc cgcatctgga agaagcgtat cgggttggcg agttgctgtt cccacatctg
     2521 gatgtcgcca ttccggaaat tccccagccg cagccgctga atccgcaagg cgaagcggtg
     2581 gcaaatgatt ttatcccccg taaagtcgcg caaagctaag gaacacgatg atggcaacgc
     2641 cagtgaagaa gtggttattg cgcgttgccc cctggttttt accggtgggc atcgtggcgg
     2701 tgtggcaact ggcctcctcg gttggctggc tgtcgacgcg tattttgcct tcaccggaag
     2761 gagtggtgac ggcgttctgg acgctctccg ccagcggcga actgtgg
//
LOCUS       DAJTRD010000226         2759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTRD010000226 DAJTRD010000000
VERSION     DAJTRD010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.228"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..197
                     /locus_tag="OO315_004198"
     CDS             <1..197
                     /locus_tag="OO315_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5892858.1"
                     /translation="LVEGGIDLKDVNVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQ
                     RGVNVFIQDVPGDQKEQIPD"
     gene            236..1039
                     /gene="agaC"
                     /locus_tag="OO315_004199"
     CDS             236..1039
                     /gene="agaC"
                     /locus_tag="OO315_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR5892859.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            1029..1820
                     /gene="agaD"
                     /locus_tag="OO315_004200"
     CDS             1029..1820
                     /gene="agaD"
                     /locus_tag="OO315_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR5892860.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            1821..2576
                     /locus_tag="OO315_004201"
     CDS             1821..2576
                     /locus_tag="OO315_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR5892861.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          685 a          640 c          682 g          752 t
ORIGIN      
        1 aattggtaga aggtggcatt gacctgaaag atgtcaacgt cggcaatatg catttctcgg
       61 aagggaaaaa gcaaatcagc agtaaagttt atgtcgatga ccaggatctc gcggacttac
      121 gttttattaa acaacgtggc gtgaatgttt tcattcagga cgtccctggc gatcaaaaag
      181 aacaaatccc tgactaaatc taaaatcgcc ttaatattgg tttgaggtaa taaaaatgca
      241 tgaaataacc ctacttcagg gattatccct agcggcgtta gttttcgttc tggggattga
      301 tttttggctg gaagccttat ttttattccg cccgataatc gtttgtaccc taactggcgc
      361 tattctcggt gatattcaga ctggcttaat taccggtggt ctgacagagt tggctttcgc
      421 cggattaacc cctgcaggtg gtgttcagcc gcccaacccg attatggcgg gtctgatgac
      481 caccgtcatt gcatggtcta cgggcgttga tgccaaaaca gcaattggtc ttggcctgcc
      541 gtttagtttg ttaatgcagt acgtcattct gttcttctat tccgctttct cattatttat
      601 gaccaaagcc gataaatgcg cgaaagaggc ggatacggca gcgttttccc ggcttaactg
      661 gacaacgatg ctcatcgtcg cttcagcgta tgcggtgatt gctttcctct gtacttacct
      721 ggcacaaggg gcgatgcagg cgctggtgaa agcgatgccc gcctggttga cccacggctt
      781 tgaagtggct ggcggtattc tgcctgccgt tggttttggc ttgctgctgc gcgtaatgtt
      841 caaagcgcaa tatatccctt acctgatcgc cggtttcctg tttgtttgct acatccgggt
      901 cagcaacctg ttgccggttg ccgtactggg cgcaggcttt gcggtgtatg agtttttcaa
      961 tgcgaaatcc cggcagcaag cgcaaccgca gcccgttgcc agtaaaaatg aagaagagga
     1021 ctacagcaat gggatctgaa atcagtaaaa aagatatcac ccgtctgggc tttcgttcgt
     1081 cgctgctgca agcgagcttt aactacgaaa ggatgcaggc gggcggtttt acctgggcga
     1141 tgttgccgat cctgaaaaag atttataagg acgacaaacc gggcttaagc gcggcgatga
     1201 aagataacct cgaatttatt aatacccacc cgaatctggt cggattcctg atggggttat
     1261 taatttcgat ggaagaaaaa ggagaaaacc gcgacaccat taaaggcctc aaagtggcac
     1321 tgtttggccc aatcgccggg attggcgatg cgattttctg gtttactttg ttgccgatta
     1381 tggcgggaat ttgctcatca tttgccagcc agggaaacct gctggggccg attctatttt
     1441 tcgccgttta cctgcttatc tttttcctgc gcgtcggctg gacccacgtc ggttattcag
     1501 tcggcgtgaa ggcgatcgat aaagtgcgag agaactcgca gatgattgct cgctcggcaa
     1561 ccatcctcgg gatcacggta atcggcgggc tgatcgcttc gtatgtgcat attaacgtgg
     1621 tgacatcgtt tgccatcgac agtacccaca gcgtcgcgct gcaacaggat ttcttcgata
     1681 aagtcttccc gaacatttta ccgatggcct acaccctgct gatgtattac ttcctgcggg
     1741 tgaaaaaagc gcatccggtg ctgttaatcg gcgtgacttt tgtgctctct attgtttgtt
     1801 ccgcattcgg cattttgtaa atggaacgag gcactgcttc tggcggtgcc tctttactta
     1861 aggaatttca tcctgtgcaa acccttcagc aagttgaaaa ctatacggcg ttaagtgaac
     1921 gagccagcga atatttattg gctgtgatcc gtagcaaacc ggatgccgtg atttgcctgg
     1981 cgaccggagc cacgccatta ctgacgtatc attatctggt agaaaaaatc caccagcagc
     2041 aggttgatat cagccagctc accttcgtga agctcgacga atgggtggat ctgccattaa
     2101 cgatgcctgg cacctgcgaa actttcctac aacagcatat cgtgcagccg ctggggctac
     2161 gtgaagacca gctcatcagc tttcgctccg aagagataaa tgagacagag tgcgaacggg
     2221 taacgaacct gattgcgcgc aaaggcggtc tggatttatg cgttctcgga ttggggaaaa
     2281 acggtcatct tgggctgaac gaaccgggag aaagcctaca accggcctgc catatcagtc
     2341 aacttgatgc cagaacacag caacatgaga tgttaaaaac cacgggtcgc cccgtgactc
     2401 gtgggatcac cttaggcctg aaggatattc tcaatgcccg cgaggttttg ttattggtga
     2461 ctggcgaagg aaagcaggat gcgacagaac gttttctcac ggctaaagtc tccaccgcta
     2521 tcccggcttc atttttatgg ctgcacagta actttatttg tttaattaat acctaaccag
     2581 atgaaaaatt tgctcaaaat ttttaaatta acttattcaa cagtcacgca ttatattaaa
     2641 taacatttga ctgggttgaa cataacgcca atagcaaaag gagttatgtt caacaattta
     2701 attttagata tacatataac ttgttctatt aataaatcac ctcgtattct gactcggcc
//
LOCUS       DAJTRD010000227         2746 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTRD010000227 DAJTRD010000000
VERSION     DAJTRD010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.229"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            10..687
                     /locus_tag="OO315_004202"
     CDS             10..687
                     /locus_tag="OO315_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5892862.1"
                     /translation="MSIIFNGHYRMKHRTWITEALRLHFEEHLPRVVAGRRLGVPKST
                     VCSMFVRFRRAGLSWPLPSGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPR
                     RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPA
                     LLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIR
                     EIKGSSH"
     gene            687..1034
                     /gene="tnpB"
                     /locus_tag="OO315_004203"
     CDS             687..1034
                     /gene="tnpB"
                     /locus_tag="OO315_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5892863.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1054..2625
                     /locus_tag="OO315_004204"
     CDS             1054..2625
                     /locus_tag="OO315_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5892864.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAEYNRLLSLRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKLLPASLPRETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPAAVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWEALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWLFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
BASE COUNT          664 a          750 c          768 g          564 t
ORIGIN      
        1 acaaaaataa tgtccatcat ttttaatgga cactatcgta tgaaacaccg gacctggatc
       61 actgaagctt tacgtcttca ctttgaagaa catttacccc gggttgtggc cgggcgtcgc
      121 ctgggtgtac caaaatcaac agtttgtagt atgtttgtgc gctttcggag agctggcctt
      181 tcgtggcctt tgccctcagg catgtcggag caggaacttg atgcctgcct ttacggacaa
      241 ttttccacgg taccagtcgt acgtcctgaa agcaccgtta tatccgaagc ccccgtggta
      301 aaaaaacgtc cccggcggcc caacttccct tatgagttta aaatcgcctt agtggagcag
      361 tcactgcagc ccggagcctg tgtggcgcag atcgcccggg aaaacggaat caacgataac
      421 ctgctcttca actggcgcca tcaataccgg aaaggtggcc tgctgccttc cggaaaaaat
      481 atgccggcac tgcttcccgt gacgttaacg ccggagccgg ataataaaat cccggctccg
      541 gcacaggaac cagagcagat aaatacaccg tccgacagtc tgtgttgtga gctggttctg
      601 ccggccggaa ctctcaggct taaaggtaaa ctgacgccgg cgttattaca gacacttatc
      661 cgcgaaataa aagggagcag ccactgatga tatctctccc tgcaggttcg cgtatctggc
      721 tggttgccgg tatcaccgat atgcgaaatg gctttaacgg cctggcctca aaagttcaga
      781 acgtcctgaa ggatgacccg ttctccggac acctgttcat cttccgcgga cgccggggtg
      841 accagataaa agtgttgtgg gctgacagtg acggactgtg cctcttcacc aaacgcctgg
      901 agcggggccg cttcgtctgg ccagtcaccc gtgacggcaa ggtgcacctt actccggctc
      961 agttatccat gcttcttgaa ggtatcaact ggaagcaccc gaaacgaacg gaacgcgctg
     1021 gaatccgcat ataacccgtt gtaaagtgag gatatggaca cctcacttgc tcatgagaat
     1081 gcccgcctgc gggcactgtt gcagacgcaa caggacacca tccgccagat ggccgaatat
     1141 aaccgcctgc tctcactgcg ggtggcggct tatgcttccg aaatcaaccg gctgaaggcg
     1201 ctggttgcaa aactgcagcg catgcagttc ggtaaaagct cagaaaaact tcgcgcaaaa
     1261 accgaacgac agatacagga agcacaggag cgaatcagcg cacttcagga agaaatggcg
     1321 gaaacgctgg gtgagcaata tgacccggta ctgccatccg ccctgcgcca gtcttcagcc
     1381 cgtaaactgt taccggcctc acttccccgt gaaacccggg tcatccggcc ggaagaggaa
     1441 tgctgtcctg cctgtggtgg tgaactcagt tctctgggat gtgatgtgtc agagcaactg
     1501 gagcttatca gcagcgcctt taaggttatc gaaacacaac gtccgaaact ggcctgttgc
     1561 cggtgcgacc atatcgtgca ggcaccagta ccttcaaaac ccattgcacg cagttatgcc
     1621 ggagcggggc ttcttgccca tgttgtcacc gggaaatatg cagaccatct gccgttatac
     1681 cgccagtcag aaatataccg tcgtcagggc gtggagctga gccgtgccac actggggcgc
     1741 tggacaggtg ctgttgctga actgctggag ccactgtatg acgtcctgcg ccagtatgtg
     1801 ctgatgcccg gtaaagtcca tgccgatgat atccccgtcc cggtccagga gccgggcagc
     1861 ggtaaaaccc ggacagcccg gctgtgggtc tacgtccgtg atgaccgtaa cgccggttca
     1921 cagatgcctg cggcggtctg gttcgcgtac agcccggacc ggaaaggcat ccatccacaa
     1981 aatcacctgg ccggttacag cggagtgctt caggccgatg cttacggtgg ctaccgggcg
     2041 ttatacgaat ccggcagaat aacggaagcc gcgtgtatgg cccatgcccg gagaaaaatc
     2101 cacgatgtgc atgcaagagc gccaaccgat atcaccacgg aagccctgca gcgtatcggt
     2161 gaactgtatg ccatcgaggc agaagtccgg ggatgttcag cagaacagcg tctggcagca
     2221 agaaaagcca gagctgcgcc actgatgcag tcactgtatg actggataca gactcagatg
     2281 aaaacactgt cgcgtcactc ggatacggca aaagcgttcg catacctgct gaaacagtgg
     2341 gaggccctga acgtgtactg cagtaatggc tgggtggaaa tcgacaacaa catcgcagag
     2401 aacgccttac ggggagtggc cgtaggccgg aaaaactggc tgttcgcggg ttccgacagc
     2461 ggtggtgaac atgcggcggt gttgtactcg ctgatcggca catgccgtct gaacaatgtg
     2521 gagccagaaa agtggctgcg ttacgtcatt gaacatatcc aggactggcc ggcaaaccgg
     2581 gtacgcgatc tgttgccctg gaaagttgat ctgagctctc agtaaatatc aatacggttc
     2641 tgacgagccg cttacttccc gacagacgac tcggtgaaaa aagtggtgtg gctggcaatc
     2701 cagtctgcgt cccagaaatg gacgatgccg ttgaaggact ggcgaa
//
LOCUS       DAJTRD010000228         2711 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTRD010000228 DAJTRD010000000
VERSION     DAJTRD010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.230"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            50..2572
                     /locus_tag="OO315_004205"
     CDS             50..2572
                     /locus_tag="OO315_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5892865.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
     gene            2583..>2711
                     /locus_tag="OO315_004206"
     CDS             2583..>2711
                     /locus_tag="OO315_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5892866.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFS"
BASE COUNT          726 a          585 c          677 g          723 t
ORIGIN      
        1 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
       61 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
      121 cgaggtcagt gtattcaatc cggcgcttct ggaaatcaac catcaatccg gagtcgatat
      181 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
      241 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
      301 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
      361 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
      421 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcacca
      481 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
      541 taatgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
      601 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
      661 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
      721 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
      781 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
      841 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
      901 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt cagccgttcc
      961 acaaggtgct tttgagatca ccgatctcaa caccgcaagt acaggtggcg atttggacgt
     1021 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     1081 gattcttaaa cgtgaaggtc tgacagatgt tgatgtcagc gtgggtgaat tgcgcgatga
     1141 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     1201 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     1261 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     1321 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aactatttga
     1381 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     1441 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     1501 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     1561 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     1621 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctggagtt attccaatac
     1681 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     1741 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     1801 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     1861 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     1921 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     1981 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     2041 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     2101 cgccaggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     2161 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     2221 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     2281 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     2341 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     2401 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     2461 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcattttc
     2521 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     2581 ttatgaacaa gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg
     2641 tagcacttgc agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc
     2701 ttgatttctc g
//
LOCUS       DAJTRD010000229         2679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTRD010000229 DAJTRD010000000
VERSION     DAJTRD010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.231"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            168..1271
                     /gene="proB"
                     /locus_tag="OO315_004207"
     CDS             168..1271
                     /gene="proB"
                     /locus_tag="OO315_004207"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5892867.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            1283..2536
                     /gene="proA"
                     /locus_tag="OO315_004208"
     CDS             1283..2536
                     /gene="proA"
                     /locus_tag="OO315_004208"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5892868.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT          637 a          678 c          768 g          596 t
ORIGIN      
        1 tggtaattta ttaaatctgt aataaaagcg taaacaactg ccgctacgct tgctgatccc
       61 gcgcaacaaa acgccatgct ttgctcgcag atggttggca accgacgaca gtcctgctaa
      121 aacgttcgtt tgatatcatt tttcctaaaa ttgaatggca gagaatcatg agtgacagcc
      181 agacgctggt ggtaaaactc ggcaccagtg tgctaacagg cggatcgcgc cgtctgaacc
      241 gtgcccatat cgttgaactt gttcgccagt gcgcgcagtt acatgccgcc gggcatcgga
      301 ttgttattgt gacgtcgggc gcgatcgccg ccggacgtga gcacctgggt tacccggaac
      361 tgccagcgac tatcgcctcg aaacaactgc tggcggcggt agggcagagt cgactgattc
      421 aactgtggga acagctgttt tcgatttatg gcattcacgt cgggcaaatg ctgctgaccc
      481 gtgctgatat ggaagaccgt gaacgcttcc tgaacgcccg cgacaccctg cgtgcgttgc
      541 tcgataacaa tatcgttccg gtaatcaatg agaacgatgc tgtcgctacg gcagagatta
      601 aggtcggcga taacgataac ctttctgcgc tggcggcgat tctggcgggt gccgataaac
      661 tgttgctgct gaccgatcaa aaaggtttgt ataccgctga tccgcgcaga aatccgcagg
      721 cagaactgat taaagatgtt tacggcattg atgacgcact gcgcgcgatt gccggtgaca
      781 gcgtttcagg cctcggaact ggcggcatga gtactaaatt gcaggccgct gacgtggctt
      841 gccgtgcggg tatcgacacc attattgccg cgggcagcaa gccgggcgtt attggtgatg
      901 tgatggaagg catttccgtc ggtacgctgt tccatgccca ggcgactccg cttgaaaacc
      961 gtaaacgctg gattttcggt gcgccgccgg cgggtgaaat cacggtagat gaaggggcaa
     1021 ctgccgccat tctggaacgc ggcagctccc tgttgccgaa aggcattaaa agcgtgactg
     1081 gcaatttctc gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc gatatcgccc
     1141 acggtgtcag tcgttacaac agcgatgcat tacgccgtat tgccggacac cactcgcaag
     1201 aaattgatgc aatactggga tatgaatacg gcccggttgc cgttcaccgt gatgacatga
     1261 ttacccgtta aggagctggc cgatgctgga acaaatgggc attgccgcga agcaagcctc
     1321 gtataaatta gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa aaatcgccga
     1381 tgaactggaa gcacaaagcg aaatcatcct caacgctaac gcccaggatg ttgctgacgc
     1441 gcgagccaat ggccttagcg aagcgatgct tgaccgtctg gcactgacgc ccgcacggct
     1501 gaaaggcatt gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg tggggcaggt
     1561 aatcgatggc ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg taccgctggg
     1621 ggttattggc gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg cttcgctgtg
     1681 cctgaaaacc ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc gcactaacgc
     1741 ggcaacggtg gcggtgattc aggacgccct gaaatcctgt ggcttaccgg cgggtgccgt
     1801 gcaggcgatt gataatcctg accgtgcgct ggtcagtgaa atgctgcgta tggataaata
     1861 catcgacatg ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc gtgaacagtc
     1921 gacaatcccg gtgatcacag gtggtatagg cgtatgccat atttacgttg atgaaagtgc
     1981 agagatcgct gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc cgagcacatg
     2041 taatacggtt gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc tgcccgcatt
     2101 aagcaaacaa atggcggaaa gcggcgtgac attacacgca gatgcagctg cgctgacaca
     2161 gttgcaggca ggccctgcga aggtggtggc ggttaaagcc gaagagtatg acgatgagtt
     2221 tctgtcatta gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca tcgcccatat
     2281 tcgtgaacac ggcacacaac actccgatgc gatcctgacc cgcgatatgc gcaacgccca
     2341 gcgttttgtt aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta cgcgttttac
     2401 cgacggcggc cagtttggtc tgggtgcgga agtggcggta agcacacaaa aactccacgc
     2461 gcgtggccca atggggctgg aagcactgac cacttacaag tggatcggca ttggtgatta
     2521 caccattcgt gcgtaaataa aaccgggtga tgcaaaagta gccatttgat tcacaaggcc
     2581 attgacgcat cgcccggtta gttttaacct tgtccaccgt gattcacgtt cgtgaacatg
     2641 tcctttcagg gccgatatag ctcagttggt agagcagcg
//
LOCUS       DAJTRD010000230         2625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTRD010000230 DAJTRD010000000
VERSION     DAJTRD010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.232"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            623..1222
                     /locus_tag="OO315_004209"
     CDS             623..1222
                     /locus_tag="OO315_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5892869.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
     gene            1595..1978
                     /locus_tag="OO315_004210"
     CDS             1595..1978
                     /locus_tag="OO315_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892870.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDSYTCHLCGSALRYHPQY
                     DTERPWFEHADEGLTEHGHECPYVRPERREIRLIKRLQQFVPDTLPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGRFSEEGGAE"
     gene            1975..2400
                     /locus_tag="OO315_004211"
     CDS             1975..2400
                     /locus_tag="OO315_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892871.1"
                     /translation="MNGIKTGLGITPGEHIISADSALSRNIRQCFCLSCHGRLILQTD
                     AQGAWFEHDLHALSEQQKADCVVLNPEKSHPYIEDMAMFLSPLPVVLEWHCVMCEQFF
                     HGKKFCEACGTGIYCRAVCTKSVYSYQPDLFEDCGGAST"
BASE COUNT          651 a          637 c          661 g          676 t
ORIGIN      
        1 ggcttcaccc gtgttaatat cctggataac tgtgtttgaa ggatttctgt taataatctg
       61 ctcctgcaac tcacctgatg agtattagtg gtaatacata atcttactcg taaaagagta
      121 taccttatgt cattaaacag tcttccggcg tgttgttcaa tcctgtacct ctgttactta
      181 tctggtacgt tgatatgtgt aatgctacgt taattgcaat atctggttat ggctttatac
      241 cttcagcatt taaaatattt tattaccgga gaaacaggga agtggaaaga cttttgggat
      301 aatgtcaggc cattctgacg ttaatcacag taccaaataa cgagagggta atggaagtta
      361 aaacctgcgt aagttcctct gttgttcact ggaaaaagtg attgcccaca cgcgttataa
      421 aggcctttcc gctgcagcct ggttaacaga gcttcaggcg ttatatgcgc tgtatggctg
      481 ttacgtatgc tgttctttgc ctggttctga tacccgtgga agtcgttacc ggaggaaaca
      541 cgttcaccct actacctgag aacatcttct tcaaaaatta cggcaacgct tcttcctgtg
      601 ttacagaaca cgaggaagaa acatgcaaac ttataacacc aatcccgctt acgaaatggc
      661 tccccagctc ctggagcact ttaaccagca tctggattca ttattcgggg tctattcgaa
      721 actgttaccg ttcagaatgg attttgctta ccgaaaaaac accctcagct acagatgtgc
      781 ctgcaggtac gccatgtgcg cggagatgct gcaactgatt aacgaggtgg gagagaaact
      841 ggtcgggtat gcctgggtga tggaatatac agaacgtaaa gggcttcaca ttcattttgt
      901 gggctatctg aacggccaga gccacaggag ttcgtatctg gtatccagat tgatgggaga
      961 tatctggagg cgggttaccg agggtaatgg ttactaccac tggtgccggt ttaataaaaa
     1021 ttatccggta aacattaacc acgtcattca ctattcagat cacaaagcgg tgaatgattt
     1081 acgctatgcc atcagctacc tggccaaacg ggaacagaaa gaatgtggca tcatcctgaa
     1141 atgcagcggg cttcctgaaa aaagtaaccg gggtcgacca cgactggaca gccccctgcc
     1201 ggggatatgt gctctggtgt aaacccctga acgacaagta cgggctggcg ctaatctttg
     1261 ctgtcggttc cggcggccac ttcagcggca ctgcgcattt tttacggcgt ggtcattccg
     1321 tatgaaccat acggagattt aaccatgaca cattctttac cattccttcc ggcaaccgat
     1381 acagcacatc ccccaatctc catctctgct tactgactgt tttttacgtc agtgggccat
     1441 tgtgtgacaa ccacagtgct gtaacagcaa ccagcctgct ttacccaaac tccagtccag
     1501 attttaactg aaccgccatt cacaccctga ccacgctgcc cgtaccggga cggctgttgc
     1561 ctgcgtgtcg tctggcggct gaggaacata tactatgtac gcaaaatctt ttctcgcact
     1621 tgatggcaac ggacgtctga ccggtgcccg taccgcacag actgcacctt acgatagcta
     1681 cacctgccac ctttgcggca gtgcactcag ataccacccg caatacgaca ctgaacggcc
     1741 ctggtttgaa cacgctgacg aagggctgac ggagcacggt catgagtgcc cttatgtcag
     1801 gccggaacgc agagaaatac ggttgattaa acgtctgcag caattcgtcc cggatacctt
     1861 acccgtagta cgtaaagcca gctggcactg caggcaatgt caccacgatt attatgggga
     1921 gcagtactgc acacactgcc agaccgggcg cttcagtgag gaggggggag cagaatgaac
     1981 ggtataaaaa cggggctggg gatcacgccg ggtgagcaca ttatcagtgc cgactctgcg
     2041 ctcagcagaa atatccggca gtgtttctgc ctttcctgcc atggccggtt gattctgcag
     2101 acagatgctc agggggcgtg gtttgagcat gatttacatg ccctgagtga gcaacagaag
     2161 gcggactgtg tggtgctgaa tccggagaag tcccaccctt acattgagga tatggcgatg
     2221 tttttatcgc cattaccggt cgtgctggag tggcattgtg tgatgtgtga gcagtttttt
     2281 catgggaaga agttctgtga ggcctgtggt acggggattt attgcagagc ggtatgtacg
     2341 aagtctgttt acagctacca gccagacttg tttgaggact gcggaggtgc gagtacttaa
     2401 tccgaaatgc ccgacctgct cagggcgttc agaggtattg ccaacctgta ttttatctgg
     2461 tgtttcgcac ccaccccgtt cgtgacacaa aggcgcgaca ccggatatcc gcagtcctgc
     2521 ttacctgaat aaaacccggg gtattgattg gtagtgtggt gcggaatcgt accgtgatct
     2581 cgcattcgcc gaattcgcac tgtggtgtca atcaaccata cgcga
//
LOCUS       DAJTRD010000231         2607 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTRD010000231 DAJTRD010000000
VERSION     DAJTRD010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.233"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..261)
                     /locus_tag="OO315_004212"
     CDS             complement(<1..261)
                     /locus_tag="OO315_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR5892872.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFA"
     gene            454..957
                     /locus_tag="OO315_004213"
     CDS             454..957
                     /locus_tag="OO315_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5892873.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(1003..1419)
                     /gene="rraB"
                     /locus_tag="OO315_004214"
     CDS             complement(1003..1419)
                     /gene="rraB"
                     /locus_tag="OO315_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5892874.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1581..2594
                     /gene="argF"
                     /locus_tag="OO315_004215"
     CDS             1581..2594
                     /gene="argF"
                     /locus_tag="OO315_004215"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5892875.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT          736 a          606 c          599 g          666 t
ORIGIN      
        1 tgcgaaatag aaaaaaacaa aaacaagaca actaacaaaa acattcccac cggtaattcc
       61 ataagaaaat cgttgtccgt taacttccat attagcataa agataacgct tacatttcat
      121 taatgcccat ggtaaataga tacctagcgt tataatcgtt aacaacacat tcaccacaca
      181 aataagaaaa tatctctcac ctgtaccatg aaaaacaaaa gaatgggacg gaacatccat
      241 ttcattaata acttgagcca taaaaatccc ctgaattaaa aaatccattg atataattta
      301 tagatacaga cagtaaaatg ttctgccacg ttacaatact cttatcagta ataggctggc
      361 aaaaacttta ttgctttata agacgcttgc gcacgtaaga agagagtggt attaaacaaa
      421 tgtataatta ctttattttt attgggttaa actatgaata acattgcgcc gcaatcacct
      481 gtaatgcgtc gcctgacgct gcaggataat cctgctatcg ccagcgtcat ccgtcaggtt
      541 tacgcagagt atggtcttac cgccgataaa ggctacaccg ttgccgatcc gaatctcgac
      601 gaactgtatc aagtgtacag ccagcctggc catgcttatt gggtggttga atacaatggc
      661 gaagtggtcg gcggcggcgg gattgcgcca ttaacaggta gtgaagcgga catttgcgaa
      721 ctgcagaaaa tgtactttct tcccgctatt cgcggtaaag ggctagcgaa aaaactggcc
      781 ttaatggcga tggagcaggc acgagagatg ggcttcaaac gctgctatct ggaaacgacc
      841 gcttttttaa aggaagccat tgcgctttat gagcatttgg gctttgagca tatcgactat
      901 gcgcttggct gcacgggcca tgtcgattgt gaagtgcgga tgctacgcga actctaaaaa
      961 aatgcccgct agtatgtgct atcgggcatt ttaatcaatt aattagtggc gaaccccgtc
     1021 atcgtcttca tcgacaaaat cttcatcgtc gccatcttcg ccgttaggat cttcaaagta
     1081 agtgccccaa ccgtcgtact caacgtcaaa tttctccgcc agcgtcatca gttgttcaac
     1141 ctgggcatcg atcagatcgg cattcaacgc gcactcgctg aggatgtcgc agcaaatcac
     1201 gatatcacca tcttcaactt ccagctcttc tggatcggtc acttcgtaac cgagtttaaa
     1261 cgcttcaact gccgcttttt ccagagtttc cagatcgtct gcggaaagat ggtgttcgat
     1321 ggtgtacagc gcgtccggat cgctgccatc ttccagtaat tcttcaataa tcaaacgtgt
     1381 ttcttcacgc tgttcttcca gttgttccgg gtttgccatg gctcattcct cttaatgtcc
     1441 tgccgatact cttattgtca cacaccactg acattgcctc cacctttgtg acaaagattt
     1501 atgctttaga cttgcaaatg aataataata cacataaatt gagttttaat tcattgaggc
     1561 gttagccaca ggagggatct atgtccgggt tttatcataa gcatttcctg aaattactcg
     1621 atttcacgcc agctgaactc aacagcctgc tgcagttagc cgcgaagctg aaagctgata
     1681 agaaaagcgg taaagaagaa gccaaactca ctggtaaaaa catcgcgctc atcttcgaaa
     1741 aagactcgac tcgtacccga tgctctttcg aagttgccgc atatgaccag ggggctcgcg
     1801 ttacttatct tggcccaagc ggtagtcaga ttggtcataa agagtcgatt aaagacactg
     1861 cccgcgtgct tggtcgcatg tatgacggta ttcagtatcg cggctatggt caggagattg
     1921 tcgaaacact ggcggaatac gctggcgtgc cggtatggaa cggcctgacc aatgagtttc
     1981 accccacgca gttgctggcg gatctcctca ccatgcagga gcatttaccc ggtaaagcat
     2041 tcaacgaaat gacgctggtc tatgcaggtg acgcacgtaa caacatgggc aattcgatgc
     2101 tcgaagctgc ggcgcttacc ggtctggatt tgcgtctggt cgcgccacag gcatgctggc
     2161 cagaagctgc gctggttacg gaatgccgcg ccctggcaca acaaaatggc gggaatatta
     2221 cgctgacgga agatgtagct aaaggagttg aaggtgctga ctttatctat accgatgtat
     2281 gggtgtcgat gggtgaagca aaagaaaaat gggctgaacg cattgcatta ctgcgtgatt
     2341 atcaggtgaa cagcaagatg atgcagttga ccggtaaccc ggaggtcaaa ttcctccatt
     2401 gcctgcccgc gtttcatgac gaccaaacga cgcttggcaa aaaaatggcg gaagagtttg
     2461 gcctacatgg cggaatggaa gtgactgatg aggtcttcga atctgccgcc agcattgtat
     2521 ttgatcaggc ggaaaaccgc atgcatacca tcaaagcggt gatggtcgcg acgctcagta
     2581 aattaaataa ttaatatcgc tataaag
//
LOCUS       DAJTRD010000232         2487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTRD010000232 DAJTRD010000000
VERSION     DAJTRD010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.234"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..>2487
                     /locus_tag="OO315_004216"
     CDS             <1..>2487
                     /locus_tag="OO315_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5892876.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIV
                     VGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYY
                     RLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQS
                     LFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERD
                     LRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQDAFWEQM
                     VNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEISSKETLAE
                     NLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGDLSFPAQQKSLIALANKAVSGSGV
                     GELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRITHSTINDN
                     IWASLQNAQIQALKTLDQRPAEKFAQLMYETRYADDRTKLLQENQIAQFTAADALAAD
                     RQLFSSPADITFVIVGNVAEDKLVALITRYLGSIKHSDSPLAAGKPLTRATDNASVTV
                     KEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASGAYSVSSR
                     LSVDPQAKDISHLLAFTCQPERHDEQLTLANEVMVKRLTKGISEQELNEYQQNVQRSL
                     DIQQRSVQQLANTIINSLIQYDDPAAWTEQE"
BASE COUNT          728 a          577 c          602 g          580 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct caggcgcgcc gccctttcct gctggctaat
      361 acccgtaatt tagaccgtga acctatcggc ctgatggata ctgtcgccac ggtcacgccg
      421 gcacaattgc gccaatttta tcaacgctgg tatcaaccaa ataatatgac ctttatcgtg
      481 gtcggcgata tcgacagtaa agaagcgctg gcgctgataa aggataattt aagtaagctt
      541 ccggctaaca aagcagctga aaatcgcgtc tggccgacaa aagccgaaaa ccacctgcgc
      601 tttaatatca ttaatgataa agaaaaccgg gtgaacggca tcgcactcta ttatcgcctg
      661 ccaatggtac aagtgaacga tgagcaaagc tttgtcgaac aagctgaatg gagcatgtta
      721 gttcagctgt tcaatcaacg tctgcaggaa cgcatacagt cgggcgagtt gaagactatt
      781 tctggcggca ctgcgcgcag cgttaaaatt gcacccgatt atcagtcgct gtttttccgt
      841 gtaaatgcac gagacgataa tatgcaggat gctgcgaatg cattaatggc agagttggca
      901 accattgatc agcatggttt ttctgctgaa gaactcgatg atgtcaaatc tacccgcctc
      961 acctggctga aaaatgcggt tgatcagcaa gctgaacgtg atttacgtat gctgaccagt
     1021 cgcctggcat ccagctcatt aaataatacg ccgttcttgt cgccggaaga gacatatcaa
     1081 ctttcgaaac gtctgtggca gcaaattacc gtgcaaagtc tggcggaaaa atggcagcag
     1141 ttaagaaaga accaggacgc attttgggag caaatggtaa acaatgagct tgccgccaaa
     1201 aaagcattgt ctcctgcggc tatcctggcg ctggaaaaag agtacgccaa caaaaagctg
     1261 gcggcttaca tcttcccagg cagaaattta tcgttaacag tagacgctga cccacaggcg
     1321 gaaattagca gcaaagaaac gctggctgag aatctgacat cattaacact ttccaatggt
     1381 gccaaggtta ttctggcaaa atccgcgggt gaagagcaaa agctacaaat tattgccgta
     1441 tcgaataaag gcgatttaag tttccctgcg cagcaaaaat cacttatcgc gctggcaaat
     1501 aaagcagtta gcggaagcgg cgttggcgaa ctctcctctt ccagcctgaa acgctggagt
     1561 gcggaaaact cggtaaccat gagcagtaaa gtcagtggca tgaatacgtt gctctccgtt
     1621 agcgcgcgga ctaataaccc tgaacctggt ttccagttga ttaaccagcg aatcacccac
     1681 agcacgatta acgacaatat ttgggcatcg ctacaaaatg ctcaaattca ggcgttgaaa
     1741 acgctcgacc agcgtccagc ggagaaattc gcccagctga tgtatgagac gcgctatgct
     1801 gatgaccgca cgaaattact gcaagaaaat cagattgcac agtttactgc cgcagatgcg
     1861 ctggctgccg atcgccagtt gttttcatct ccagcggata tcacgtttgt cattgtcggt
     1921 aatgtcgcag aagacaaact cgtggcgtta attacgcgtt acttaggctc aatcaaacac
     1981 tctgattcgc cattagccgc aggtaaacca ttaactcgcg cgacggacaa cgcatcggtt
     2041 actgtaaaag aacaaaatga acctgtggca caggtttcac agtggaagcg ttatgattcc
     2101 cggacacctg ttaatctggc gacgcgtatg gcgctcgatg cttttaacgt cgcactggca
     2161 aaagatctac gcgttaatat tcgtgaacag gcatctgggg catacagcgt ttcttctcgc
     2221 ctctcggttg atcctcaggc taaagatatc agtcatttgc tggcttttac ttgtcaacca
     2281 gaacgacatg atgaacagtt aacgttagcg aatgaagtga tggttaagcg tttgactaag
     2341 gggatcagtg agcaagaact gaacgaatac cagcaaaacg ttcagcgcag cctcgatatc
     2401 caacagcgta gcgttcaaca attagcgaac actattataa atagtcttat tcaatatgac
     2461 gatcctgcag catggactga gcaggag
//
LOCUS       DAJTRD010000233         2383 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTRD010000233 DAJTRD010000000
VERSION     DAJTRD010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.235"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            40..1437
                     /gene="ccp"
                     /locus_tag="OO315_004217"
     CDS             40..1437
                     /gene="ccp"
                     /locus_tag="OO315_004217"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5892877.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1648..>2383
                     /locus_tag="OO315_004218"
     CDS             1648..>2383
                     /locus_tag="OO315_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5892878.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVPTFGVTYTGNYE
                     FPQPLHDALDKFQADTGIDIDMHIDAA"
BASE COUNT          639 a          522 c          616 g          606 t
ORIGIN      
        1 ttctgggtca caataactca ccaccgtgag ggaagaataa tgaaaatggt ctcacgtatt
       61 accgcgatcg gcctggctgg cgtcgcgatt tgctatttag ggttatctgg ttatgtgtgg
      121 taccacgata ataaacgcag taaacaggcc gatgttcagg catctgctgt cagtgaaaat
      181 aataaggttt taggctttct ccgcgaaaaa ggatgcgact attgccacac gccttcggca
      241 gaattacccg cctattatta tattcctggc gcgaaacagt tgatggatta cgacattaag
      301 cttggatata aatcttttaa cctcgaggcc gtgcgtgcgg cactgctggc tgataaaccc
      361 gtttcgcaaa gcgatctgaa taagattgaa tgggtgatgc agtatgaaac tatgccacca
      421 acgcgttata ccgcgctaca ctgggcgggt aaggtgagtg atgaagagcg ggcggaaata
      481 ctggcctgga ttgcaaaaca gcgcgcggaa tattacgcca gcaatgatac tgctccggag
      541 catcgcaatg aaccggtgca gcccatcccg caaaaactgc ctaccgatgc gcaaaaagtg
      601 gcgttgggct ttgcgctgta tcacgatccc cgtttatcgg ctgatagcac catttcatgc
      661 gctcattgcc atacgttgaa tgcggggggc gtcgatggca gaaaaacatc gattggtgtt
      721 ggtggcgcag ttgggccgat taatgcgccg acggtattta actcagtatt taacgttgag
      781 cagttctggg atggtcgtgc ggcaacattg caggatcagg caggtggacc gccgttgaac
      841 ccgattgaaa tggcgtcgaa atcctgggac gaaattattg ctaagctgga aaaagatccg
      901 cagcttaaag cgcagttcct cgaagtttat ccgcaaggtt tcagtggcga aaatattact
      961 gatgccattg ctgaatttga gaaaacatta attacgccgg attccccatt tgataaatgg
     1021 ttgcgtggag atgaaaatgc gctgacggcg caacagaaaa aaggctatca attatttaaa
     1081 gataataaat gtgcaacttg tcatggtggt attattctcg gcggacgttc ctttgaaccg
     1141 ttggggctga aaaaagactt taactttggg gaaattacgg cggcggatat tggtcgtatg
     1201 aatgtgacta aagaagagcg tgataaattg cgtcagaaag tacccggttt acgtaacgtt
     1261 gctttaacgg caccgtactt ccatcgcggt gacgtgccga cgctggacgg ggcggtgaaa
     1321 ctgatgctgc gctatcaggt aggcaaagag ctgccgcagg aggatgtgga tgatatcgta
     1381 gctttcctgc acagtctgaa cggggtgtac acgccgtata tgcaggataa acaataatta
     1441 atttgatcgc ccgaacagga atgtttgggc gatttttatt acgataataa agtctgtttt
     1501 taatattatc atgttaaatg tttatattat aaaaagtcgt ttttctgctt aggattttgt
     1561 tatttaaatt aagcctgtaa tgccttgctt ccattgcgga taaatcctac ttttttattg
     1621 ccttcaaata aatttaagga gttcgaaatg gaccagaagc tgttaacgga tttccgctca
     1681 gaactactcg attcacgttt tggcgcaaag gccatttcta ctatcgcgga gtcaaaacga
     1741 tttccgctgc acgaaatgcg cgatgatgtc gcattccaga ttatcaatga tgaattatat
     1801 cttgatggca acgctcgtca gaacctagcc actttctgcc agacctggga cgacgaaaac
     1861 gtccataaat tgatggattt gtcgatcaat aaaaactgga tcgacaaaga agaatatccg
     1921 caatccgcag ccatcgacct gcgttgcgta aatatggttg ccgatctgtg gcatgcgcct
     1981 gcgccgaaaa atggtcaggc cgttggcacc aacaccattg gttcttccga ggcctgtatg
     2041 ctcggcggga tggcgatgaa atggcgttgg cgcaagcgta tggaagctgc aggcaaacca
     2101 acggataaac caaacctagt gtgcggtccg gtacaaatct gctggcataa attcgcccgc
     2161 tactgggatg tggagctgcg tgagatccct atgcgccccg gtcagttgtt tatggacccg
     2221 aaacgcatga ttgaagcctg tgacgaaaac accatcggcg tggtgccgac tttcggcgtg
     2281 acctacaccg gtaactatga gttcccacaa ccgctgcacg atgcgctgga taaattccag
     2341 gccgacaccg gtatcgacat cgacatgcac atcgacgctg cca
//
LOCUS       DAJTRD010000234         2258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTRD010000234 DAJTRD010000000
VERSION     DAJTRD010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.236"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..796
                     /locus_tag="OO315_004219"
                     /pseudo
     CDS             1..796
                     /locus_tag="OO315_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            911..1252
                     /locus_tag="OO315_004220"
                     /pseudo
     CDS             911..1252
                     /locus_tag="OO315_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1235..2072
                     /locus_tag="OO315_004221"
                     /pseudo
     CDS             1235..2072
                     /locus_tag="OO315_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(2122..>2258)
                     /locus_tag="OO315_004222"
     CDS             complement(2122..>2258)
                     /locus_tag="OO315_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5892879.1"
                     /translation="RAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTFYTPQGS
                     "
BASE COUNT          549 a          548 c          683 g          478 t
ORIGIN      
        1 gggccttgcc cgtagcagtt atttttatca ccgggctgca ctgaaagccg gtgataaata
       61 cgccacgata cgtaagatgc tgacagatat ctttaacagt aattaccagt gttatggcta
      121 tcgtcgcctg catgcgatgc tcaggcatga gggggggcgg ctatcagaaa aggttgtacg
      181 cagacttatg gtggaggaac agcttgtcgt cagtcgtaac cgtcgtcgtc gctacagctc
      241 atattgcgga gaaatcggac cggctccgga taaccttatc gccagagatt ttaaggcgga
      301 gcaacctaat cagaaatagc tgacagatat cacggagttc cagctccctg caggtaaagt
      361 ctggctgtca ccagtggtgg actgcttcga tggaaaagtt gtgagctggt ctctcagtac
      421 acgccctgat gctgaactgg tcaacactat gctggatagc gctgtcgaaa cgttaaatgc
      481 tggcgaacga ccggtgatac acagtgacag aggtgggcat tatcgctggc caggctggct
      541 ggaaagagtg aatgcagcag gtcttattcg ctcaatgtcc cgtaaaggat gttcacctga
      601 taatgctgca tgcgaaggct ttttcggcag actgaaaacg gaaatgtatt atgggcgtaa
      661 atggtcgggc atcacgccag aaaagttcat gcagcaagtg gatgcttaca tcagatggta
      721 taacgagcgg cgtataaaat tatcgctggg tgccgtcagc cccaaaatgt accgccaaca
      781 atgcgggctg gaatgataaa gcagtccagg aaatcgtccg catcccccct tcaccatttg
      841 gattgtccgc tccacccaac atgttgtttc cttaaggttc ctacaccaca aaggaaactc
      901 tcatgctgcg cagtggatgg ctgacgttgc cgacaggcgg gaacttcgcc agttcaggca
      961 gacaccggaa cagcgcttca cgcaggaaca ggagcatctg cagccgctgc tgggtacgga
     1021 cttcgatatc cgtcatgtct cctgggatgg ctatatcgag gttggcggga accgttacag
     1081 tgtgccggaa agcctgtgtg gtcagctggt atcaatacgg atctcgctgg atgatgaact
     1141 gcggatctac agtaatgagc aacaggtagc atcgcaccgt ctctgttcgg ctgcatacgg
     1201 ctggcagacc gtgccggagc atcacgctcc actctggcag caggcttcgg agcgcatggc
     1261 cgaatggctg ggggagatac agaaacgggt aatcactgtc tgtgaccggg aagcggatat
     1321 ctggcattac ctgtattata aagtcagcca cggacagcgt ggtgcgtgct gcacagaatc
     1381 gccggctgga agaggcaccc ggcaagctct tcgaactgcc ggaagtcctg gcaaccgcag
     1441 gaagccacac gctgaatgtg atgcaaaaag gcgggcgggc agcccgtcag gcccggatgt
     1501 tcatcagtta cagcgaagtc agcataaaaa atcccgacaa cagcggccag gcgctcccgc
     1561 tcacgtatgt ctgttgccgg gagcaggcag aggacggtgc ctgctggcat ctgctgacgt
     1621 caggaaaagc ggcgagtgcc gccgatgcac gacgtattgt cagccattac gagcgacgct
     1681 ggctgacaga ggaataccac aaggcgtgga aaagtggtgg tacatggaat cgctgcgaat
     1741 gcagacccgg gataaccttg agcgcatggt ggttatcctg gcgtttatag cggtacgggt
     1801 gctgggcctg cggcagggag gtgtcagtga agaaacacaa aatgacagct gtgagaaaat
     1861 actgacgccg acagagtgga aactactgtg ggttaagctg gaaggaaagc cgcttccggt
     1921 gcaggcaccg accctgaaat gggcctgggg cgatggcatg acagcaaacg cacaggtcgt
     1981 cccggttggg gcgtcatgtg ggatggctgg tcagacttca ggatatggtt gagggttacc
     2041 tggtgatgaa gtctcttgat caggagctct gatcaagaga cagcagctta ggggggtgcc
     2101 cctattaagt tgtcaagcat gttatgaccc ctgcggggta taaaaagtcc cgtcgcgcat
     2161 catggcgaac agaacgtcgc agcgtcgtct cgccagggcg ataagcgcct gattgtgtcg
     2221 ttttccctga ctcattttgc gggtgtagta agccctgg
//
LOCUS       DAJTRD010000235         2244 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTRD010000235 DAJTRD010000000
VERSION     DAJTRD010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.237"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(158..670)
                     /locus_tag="OO315_004223"
     CDS             complement(158..670)
                     /locus_tag="OO315_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5892880.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            865..1629
                     /locus_tag="OO315_004224"
     CDS             865..1629
                     /locus_tag="OO315_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892881.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1580..>2244
                     /locus_tag="OO315_004225"
     CDS             1580..>2244
                     /locus_tag="OO315_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR5892882.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          643 a          489 c          565 g          547 t
ORIGIN      
        1 atcatcagcg accagacagc attgagaatg cttcaggagt acatcaggat ccaatgcctt
       61 ggggggtagc ggtaatttta ctcattatcc ttcaaatcag attctgttgt cagaggaatg
      121 ggggaagctg gattcgcaag agctttttat gggcagttta gctaaaaaaa ggcaacacga
      181 agctaaaact accaacataa agtgctgacc agctagcagc tgaaatcaga ctcactgtat
      241 acactttcac tggatgaagt tgcaacattc ctgccaccaa aggagcaatg taacgcagca
      301 cggcgataaa gcgcgaagtg aaaagtattg acacagagtt attttgcaac tgaaggcgaa
      361 cccgttctat tgtattggaa cggctcgata ttatacatgc aagttgaggg atatgcccaa
      421 ttattatacc aagatggtaa ttaacgatag taccgcacca tgcgccactc atgaccgtga
      481 ttccagcttc ccatggtgaa agagtcgtct tgctgactgt aattacagtg gccatcataa
      541 ttgatgctgg tggcaatatc gccgaaatga gtaaagtgga ttttgtgaac gctattataa
      601 aaagaagccc ccataggcga tatggatgaa gtacaaaata gttcatcaaa gcatttatcc
      661 actccatcaa tgtctttcct tctgtgaatt tttctttgct atttaaccgt gatacgactt
      721 aatttaggct taaacacctt acatttacaa ggtactcctg tggggggccc tgccacggag
      781 cgtcgcagga gtgagtgatg aggaagaatt aaagcaacct attgtgacag gcagcgataa
      841 cagtaaatca caggaagcat aatcatggca aaaccggact gggaggccat cgaatcggca
      901 taccgggccg gagtccttag cctccgtgat ataggcgaga aatacggcgt tacagaaggg
      961 gctatcagga agagggccaa aaagcttgat tgggcacgca gtggcggtac gcaggtttgc
     1021 aaaaatggta cgcaaaaaag gaaagtgcgt accagcagaa agcctgccat tactggcctt
     1081 acacaaaaaa gtacgcaact aaaaacagaa tctacaccgg atacgaaacc gatacgcgga
     1141 atgcgtaccg atcccccgac taacccattc caacccggta accagcaggc attaaaacac
     1201 ggtggttatg cccgtcgcct tctgctcaaa gatgaggtga tagaggacgc taaagcgttg
     1261 acgctcgagg acgaattatt tcgccttcgt gctaacaacc ttgtcgctgc agagaatatt
     1321 ggtcggtggt tggtgtcgct ggaagatgct aatggggacc aggaaaggaa gatgctgatt
     1381 gaaaatatca gcgctgccga gaaagcaatg atgcgcaata cagttcgtat tgagtccatc
     1441 gttggcacgc ttgcgacggt aggaaaaata tttgctgata cagcctaccg caaggccgcc
     1501 actgataagg tgtctctgga ggctgatcgt cttcgccgtg atgcaggtat tgatgatggc
     1561 aatggagagc gtgacctcaa tgacttctac tctgacatcc aaaccgacgc tgaatccggc
     1621 tttacgtagt ttctggacta tgcgggcacg taacaaagtg ctttatggtg gtcggtcatc
     1681 gtcaaaatca tgggatgccg ctggcattgc catatttctg tcgaataaat acaccctgcg
     1741 tttttgttgt gcccgtcaga tccagaataa aatcgaagag tcggtgtata ccctgctcaa
     1801 aattcagata gacaggtttg gtctgcggca ccgtttccgt attctgaaca acaaaatcat
     1861 taaccgggtt actggctcgg aatttgtttt ttatggatta tggcgcaaca tcgaagaaat
     1921 taagtcactg gaggggatcg atatgttgtg gctggaagaa gcccacgcac tgacggaata
     1981 ccagtggaaa attctggagc caacgatccg taaagagggt tcggaatgct ggttcatatt
     2041 caaccccgga cttgttactg atttcgtctg gcgcaacttc gttgttgatc cgcccgaagg
     2101 cactctcatc cgcaaaatta actatgacga aaatccgttt ctgtctgaca ccatgcttaa
     2161 ggttatcgac gcggcgcgac gccgtgatcc ggatggtttt aaacatgtgt atgagggcgt
     2221 tccggagtct gatgatgatg cggc
//
LOCUS       DAJTRD010000236         2219 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTRD010000236 DAJTRD010000000
VERSION     DAJTRD010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.238"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO315_004226"
     CDS             complement(100..525)
                     /gene="arsC"
                     /locus_tag="OO315_004226"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5892883.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO315_004227"
     CDS             complement(538..1827)
                     /gene="arsB"
                     /locus_tag="OO315_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5892884.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1881..>2219)
                     /gene="arsR"
                     /locus_tag="OO315_004228"
     CDS             complement(1881..>2219)
                     /gene="arsR"
                     /locus_tag="OO315_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5892885.1"
                     /translation="PIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISR
                     HLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQN
                     CSGDSKNICS"
BASE COUNT          589 a          611 c          556 g          463 t
ORIGIN      
        1 gaatttgcac ggttttgtag gtcggataag acgcgttagc gtcgcatccg acataaacaa
       61 agcgcacttt accaactatc ggcagtggat atcgccgctt tatttcaggc gcttacccgc
      121 ttcatcaacc actttctcgc catcttcctt ggagaatgcg cctttttgcg catctggcag
      181 aatttccagc accacttctg aagggcggca caggcgagtt cccagcggcg tcaccacaat
      241 cgggcgatta atcagaatcg ggtgctgaag cataaagtcg attaaccgat cgtcagtaaa
      301 tttatcttcc gcaaggccca gctcctcata cggttcgacg tttttacgca gcagcgcgcg
      361 tacggaaatc cccatatcgg caatgagttt gaccagttca tcgcgcgttg gcggagtttc
      421 cagataatgg ataatggtcg gttctgtgcc gctgttgcgg atcatctcca gcgtattacg
      481 cgacgtgccg caggccgggt tgtgataaat ggtaatgttg ctcatatcag tatctcatta
      541 caaagtgaaa gagagacgta gcgccagcgc agccagcgtc acaaacagca caggcagggt
      601 catgataatc cctgtacgga agtaatatcc ccagctgata gtcatattct tctgcgaaag
      661 tacgtgcagc cagagcagcg ttgccaggct accaatcggg gtaattttcg gccccaaatc
      721 gcagccaatc acgttggcat aaaccatcgc ttctttgata acgccagatg ccgtgctgcc
      781 atcaatggac aacgcgccaa ccagtaccgt cggcatattg ttcataatag aagagaggaa
      841 ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg ccgttatccg ccagcacgtt
      901 gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc aggccataaa ccaccagata
      961 catgccgagc gagaagatga caatctgcca gggggcaccg cgcaggactt tacccgtatt
     1021 aatcgcatga ccgcgtttag cgacgacaaa taatatcagt gcgcccacag ctgcaatggc
     1081 gctcaccgga atgccgagcg gttccaggac gaaaaatccc accagcagaa gcagtaaaac
     1141 aacccagcca gttttgaacg tagcaggatc tttgatcgct tctgcgggag atttcagcag
     1201 cgccatatcg tagttctgcg gaatatcttt gcgaaaatag agatgtagca tcaccagcgt
     1261 ggcaacaatc gcggcgatat ccaccggcac catcaccgag gcgtattcgc gaaagccgag
     1321 gccaaagaaa tcagcggaaa cgatattcac caggttggag acaataagcg gcaggctggc
     1381 ggtatcggca atgaatccgg ccgccatcac gaacgccagc gtcgtgcctt tactgaatcc
     1441 taaagccagc agcatggcga tgacaatcgg cgtcaaaata agcgccgcgc catcattggc
     1501 aaacagggcc gcaacggcgg caccgagcag gacaatccag gtaaacagca agcgaccgcg
     1561 tccattgccc cagcgtgaaa cgtgcagcgc tgcccattca aaaaagccgg actcatccag
     1621 cagcaggctg atgataatga cggcaataaa cgccgccgtc gcgttccaga cgatattcca
     1681 caccaccgga atatcacccg gatgaaccac gcccgtaacc aacgccagta ccgcgccgag
     1741 cgttgcactc cagccgatgc ctaaaccttt cggctgccag ataaccaata cgatggtcag
     1801 gacaaagata gcgcctgcca gtaacataat gcctcccgga taaaacacat gtgcaaatcc
     1861 atatgtgttt agctaaattt ttaactgcaa atgttcttac tgtctccgga acagttttgt
     1921 ctggccaggt tgcggacaat tgcctgaatc ttttcctgtt cacatcgcca ggcttcatca
     1981 ataattttcg ccgcccatgc tggaatatgc ggcgataagc ggtaatgaac ccacttacct
     2041 tgcttgcggt ccagcaatag cccgctttca cgcagcaatg ccagatggcg ggatatcttg
     2101 ggctgcgact gatcgagggc agtgcagaga tcgcagacgc ataactctcc cagttcgcta
     2161 agcagtaaaa cgatgcccag acgggtttca tcagcaagaa ttttgaacaa ttggatggg
//
LOCUS       DAJTRD010000237         2187 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTRD010000237 DAJTRD010000000
VERSION     DAJTRD010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.239"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(220..384)
                     /locus_tag="OO315_004229"
     CDS             complement(220..384)
                     /locus_tag="OO315_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892886.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1286..1570
                     /locus_tag="OO315_004230"
     CDS             1286..1570
                     /locus_tag="OO315_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5892887.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1558..2043
                     /locus_tag="OO315_004231"
     CDS             1558..2043
                     /locus_tag="OO315_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5892888.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          565 a          462 c          620 g          540 t
ORIGIN      
        1 tcaaaatgat ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga
       61 gccggttact ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt
      121 ctgcaatacc ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata
      181 gctgatggag ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc
      241 gtttccggcg taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt
      301 atgagagatg aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt
      361 aagagcggtg gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt
      421 gcggagatga acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc
      481 gaaaatgtgc tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc
      541 ggcatccggc atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc
      601 tgtcgtctcg atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg
      661 atgcgagaca caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg
      721 agcgaagggg gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc
      781 catgagtcag ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg
      841 ggtcaaagct aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt
      901 taccaatcga agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt
      961 gcccgcaaac acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga
     1021 attcgtcacg actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg
     1081 gttcaacgac aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg
     1141 ggggcggtgc ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta
     1201 aatgccgctg acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg
     1261 tcaatatgct attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca
     1321 gctcaatctc agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct
     1381 tcataagtcg cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat
     1441 tctcgaccgg cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct
     1501 tgatgctccg gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg
     1561 ggatgtgtaa ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc
     1621 agtgaagctg tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga
     1681 gccacccgga cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca
     1741 ctggccactg gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca
     1801 gaccccattc ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa
     1861 gggcttggcg cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat
     1921 atcggtgtgc gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt
     1981 catcatggtt ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa
     2041 tagggagtaa tgccgatcag ttaaggatca gttgaccgat ccagtggctg tgtaagaatc
     2101 cggaaacgct cactcgtttc cggatttttc tgtgcacagt aaaagaccac ctcctgagga
     2161 ggtggctttt cagaatgccc cctataa
//
LOCUS       DAJTRD010000238         2185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTRD010000238 DAJTRD010000000
VERSION     DAJTRD010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.241"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..695
                     /locus_tag="OO315_004232"
                     /pseudo
     CDS             1..695
                     /locus_tag="OO315_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            697..1302
                     /locus_tag="OO315_004233"
     CDS             697..1302
                     /locus_tag="OO315_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5892889.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            1289..>2185
                     /locus_tag="OO315_004234"
     CDS             1289..>2185
                     /locus_tag="OO315_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5892890.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNAGFAGAIGGYAKGAIL
                     AGVATTAVWLNTTDDNLTDPEGSDSAGWVNLLEDPKRIFLRQKNNLSDLQNKGTARDN
                     LQVYSKEQSDQRYVHREGDKITGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSE
                     GQGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGD
                     NDTGFKQNGDGILDVYANSQRVF"
BASE COUNT          531 a          508 c          610 g          536 t
ORIGIN      
        1 gcccgtgccg gtgtgccggg cagcgatgag gaaacccgtg cggactttga aaaccgccga
       61 cgtaattcag ttgcccgtaa tgcccggaat attctggaag ccatccgggg tgaaatactc
      121 tccacggtag aaaacgtggt ggatgtttac gtcacccata atccgaaaaa aacagaacaa
      181 aaagccggag tcagtatcca ttaacacccg gttcgcttta tgttggcgtg tacggcggca
      241 gcccggcaga tatcgcggcg gccatctggc gtaaggctcc gccgggtatt gatatgaatg
      301 gcaacacaac gttcactgtt gcagataagg agtacgatcc gccgtatcct gaatacgtga
      361 tcagctggca gacactcaaa cccgtcagtc tgcatgtcag tgtgacgctg aaaaaaagtg
      421 actacctgcc ttcagatatt acccgacagg tacagcaatc cgtgctggac gcatttaatg
      481 gtacagatgg tggtctgcgg gcaagagttg cctctgttgt ctctgccggg cgctactatg
      541 ccggcattta caaaaccgat ccggaacata ttgatattct gggccttact gtgagccgtg
      601 atggttcgtc atggacaacg gctgtcactt tcgggataga tgagattccg gttctggatg
      661 tgtcggatat cagtgtgaaa ctccaggagg cataacgtgc agaatgtggc tgccactgtg
      721 cttgcgcagt atgctgccag cccccgactc aatgccctca ttaacagctt taacgcagcg
      781 ctttcccccg acagttttat cagtgatttt tatggtctta tctggaacat cgataccgca
      841 gaaaagtatg gtcttgatgt ctggggaaag attgtgggtg tcagtcgctg gctgacggta
      901 aaggacgatt ttaattacct gggcttcagc gagtccagga tggacacccc ggtaatggat
      961 gatccctgtc cgtttaatca ggcaccgttt tacaacggaa aatcggatac ccggactgtt
     1021 gacctgtctg atgctgtata ccggcggctg atactgatga aagccatgtc gaacattact
     1081 gactgttccg ttccggatat taacctgatg ctgagattta tgttcggaaa aaaacgccgg
     1141 gcttatgttc tgaataatgg cgggctgagg atgagttacg tctttgagtc cgcgctctcg
     1201 ttggcagaac tggcgattat ccagtcgtcg ggtgcactgc catccccgcc gggtgtttat
     1261 gtttcagtag ttttaaagga gtcccgtaat gaaggccagt gataaacccc gccagctggc
     1321 ggtccccttt gcgagtaccg gagataaaaa ccgtatcccg gacaaggcga cacagcagac
     1381 cagagagagc ggtaatgctg cgtatgattc aggttttcct ccggtgacca tgacagcggt
     1441 ctcagcggga ggtataccgc cacacggcaa ggattttaac ggtctgatgt acgatattac
     1501 cgcagcaata cggttcgccc aggctggcgg tttgtacacg tataatgccg gttttgcggg
     1561 ggccattggt ggatatgcaa aaggagccat tctcgccgga gtcgcaacaa cagcggtctg
     1621 gctgaatacc acggacgata acctgaccga tcctgaaggc tccgacagtg cgggctgggt
     1681 aaatcttctt gaggatccga aaaggatatt cctgcggcag aagaacaatc tgtcagacct
     1741 tcagaataaa gggacggcac gggataatct tcaggtttac agtaaagagc agtcagatca
     1801 acgctatgtt catcgggaag gcgataaaat aaccggagag ctgaaaatcc gtggtgttaa
     1861 tgcgctgagg attttcaacg aagcttttgg cctgattttt cgtcgttcgg aagagtgcct
     1921 gcaccttatt cccaccagtg aaggtcaggg ggaaaatggc gatattggtc ccctgcgccc
     1981 gttcaccatt aatttgcgga cgggtgaaat atccatgtcg cataaagtgt ctgttggcgg
     2041 tggttcgcag gtcaatggtg cgctgggtat cggcgttcag aacgccctgg gggggaattc
     2101 aattgttctt ggtgataatg acaccggatt taaacagaat ggagacggta ttctggatgt
     2161 ttatgctaat agtcagcgtg tattc
//
LOCUS       DAJTRD010000239         2151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTRD010000239 DAJTRD010000000
VERSION     DAJTRD010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.242"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO315_004235"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO315_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5892891.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OO315_004236"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OO315_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1982)
                     /locus_tag="OO315_004237"
                     /pseudo
     CDS             complement(1292..1982)
                     /locus_tag="OO315_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1905..>2151
                     /locus_tag="OO315_004238"
     CDS             1905..>2151
                     /locus_tag="OO315_004238"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892892.1"
                     /translation="MPGTTTAMSINFIGITARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAI"
BASE COUNT          630 a          398 c          493 g          630 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgttgc gccttgctgc caacgaagcg ccactgctca tcaagttcac agataagggc
     1681 aacatcagcg tgagcgactg gcgcagatgt tatccgccgg ggcgagagct ttttaatgtc
     1741 cgtatgacgg tattgatgcc gattttcagt gtcctggcgg tatcacggcc ccctgcgccg
     1801 ttatgtgcca tttcaacgat ctgctcctta acgccgagtt ttctggcttc ataagagtaa
     1861 gtgagctgga aaacacgttt acatgagcga caacggaaac gctcatgccg ggaacaacta
     1921 cggccatgtc gataaacttc atcggaatta cagcgcggac aatgaacagt aacggtagtc
     1981 atggaaaacc tcaaatacca gtagattacc agaaacttct atcgattgaa gacattacct
     2041 tttgccgcaa taggtggggt aacattggtg aaaacgcatt acgccgggtg gcagttggaa
     2101 aaaaattatc ttttttcggc tctgatcgtg gtggagaaaa tgcggcaata a
//
LOCUS       DAJTRD010000240         2125 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTRD010000240 DAJTRD010000000
VERSION     DAJTRD010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.243"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..331
                     /locus_tag="OO315_004239"
     CDS             <1..331
                     /locus_tag="OO315_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892893.1"
                     /translation="GWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDV
                     RYGHEMYYSPGSNTVSWRFCAPSGHGLSGMAISDTGRNSADNVDGVYYRPLQKLINGT
                     WYNVASI"
     gene            334..879
                     /locus_tag="OO315_004240"
     CDS             334..879
                     /locus_tag="OO315_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5892894.1"
                     /translation="MLHLKNITAGNPKTAEQYQMTKRYSVTWLFSEDGKNWYEELKNF
                     ASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEFV
                     FDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDFS
                     TITDKSSYNAIEWPASPEGSS"
     gene            903..>2125
                     /locus_tag="OO315_004241"
     CDS             903..>2125
                     /locus_tag="OO315_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5892895.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAKNDIDAALTGTLKTAN
                     HLSETAAAGEKAQQKSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGINGPIYHTRYCYWPISRLTGWVKINITTEDIIYRIVASSVRNRWGDPDIG
                     GLIIAAYQGEADGDKVIRLVRGQSYRGSRLGPVGISVPSTPTGTYIASPQFFITGCSE
                     HSLPGSYCALSGVPDA"
BASE COUNT          572 a          500 c          585 g          468 t
ORIGIN      
        1 aggctggcat tttttcagcc agagaaaccc ggataacagc atagtgttca ctgttaacgg
       61 acaggtaatt ccgttaaatt acggaaactt cgatgcccgc tataaatatc gaacagaggg
      121 ggtacaggat gtacggtatg gccatgaaat gtattacagc cccggcagta acaccgtttc
      181 gtggagattt tgcgcacctt cgggacacgg gctgtcaggg atggcgatat cggataccgg
      241 ccgtaactca gcggataacg ttgacggtgt gtattaccga ccactgcaaa aactgattaa
      301 tggcacctgg tataacgtag cgagtattta acaatgttgc atttaaaaaa tattactgcg
      361 ggtaatccga aaacagcaga acaatatcag atgacaaaac gatattcggt cacctggctt
      421 ttttcagaag acggaaaaaa ctggtatgaa gagctgaaga atttcgccag cgacacaata
      481 aaaatagctt acaccggaga tggtcgcgtg gtgtgggtcg gtaaggatgt gacaggcatc
      541 gagccacgca atgccagcgt tatcgaagtt ccggatatta ccgctaaccg acggattacc
      601 gcgccgggtt actggtttta ccgcaatgat gaatttgtct ttgactacag actcaaagcg
      661 gaagatgagc gtgatgccct tctggctcag gtcagtgccc ggacagggga atgggaagaa
      721 gacctgctgc tggggttaat cagcgacgaa gataaagaaa agctgaaagc ctgtcgtatt
      781 tacgcgaaat cgctgcaggc gatggatttc agcaccatca ctgataaatc ctcatacaac
      841 gccattgaat ggcccgcctc tccggaaggt tcttcctgat ttaatttatc gcgagaaaaa
      901 caatgtctgt agtgatatca ggtgcgctga ctgatggcgc aggtatcccc atgtccggat
      961 accatattat tctgaaatcc cgggtaaaca ccccggaagt ggtgatgaac actgttgctg
     1021 atgtgatgac aggaaacgat ggtgaatact gtttccatgc gcggactgga aaatatggtg
     1081 tgtatctgaa acaggactgg cgcaacgagt acaacgttgg cgacattgct gtatatgagg
     1141 actcaaagcc cggcacgctg aatgactttc tgattgctcc tgatgagggc gacctgaaac
     1201 cggatgtcgt caaacgcttt gaggaaatgg tggcgcaggc gcagcagagc gccggggccg
     1261 cagccggaaa cgcacagcag acggcgcagg atgtggcggc agccgcaggt tatgcccgcg
     1321 cagcagagca ggccaaaaat gacattgatg ctgcgctgac cggcactctg aaaacggcta
     1381 accatctgtc tgaaaccgca gcagcaggcg aaaaggcaca acagaagtcc cgggataatc
     1441 tggggctgaa aagtgcggcc acgatggaag cacagagcga catttacgac cggacaaaag
     1501 gccgtctggc gatacccggc gcattcggct ttgggtgtgc ttttctgcct gaagatgtta
     1561 tccgttttga cactaagagt gatttcctgg cctgggtaag gaatgcgctg ccaggtgaat
     1621 attccgttgc tggcccctac ggcatcatca tacccgacac acggtttgaa ggggtgctca
     1681 gcatccggtg gactgatgca cgccctgaga caacagaacc gcggtacaga gccaaatccc
     1741 ttacttttta cggcattaac ggccccattt atcacacccg ctactgctac tggcccatat
     1801 ccagactgac tggctgggtg aaaataaata taaccacaga agatattatt tacagaatcg
     1861 tggcgagctc tgtccgcaac agatggggag accctgacat tggcgggctg attattgctg
     1921 cgtaccaggg agaagctgac ggtgataaag tcatcagact tgtcaggggg cagtcataca
     1981 gaggctcacg actgggaccg gtggggattt cagtgcccag tactcccacc ggaacgtata
     2041 tagcatcccc acaatttttc attacgggat gttcagagca ttcattaccg gggtcatatt
     2101 gcgccctgtc cggggtgccg gatgc
//
LOCUS       DAJTRD010000241         2124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTRD010000241 DAJTRD010000000
VERSION     DAJTRD010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.244"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..170)
                     /locus_tag="OO315_004242"
     CDS             complement(<1..170)
                     /locus_tag="OO315_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892896.1"
                     /translation="MRKLKMMLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPP
                     SNSLQLLDETFSV"
     gene            complement(387..920)
                     /locus_tag="OO315_004243"
     CDS             complement(387..920)
                     /locus_tag="OO315_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5892897.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(986..1615)
                     /locus_tag="OO315_004244"
     CDS             complement(986..1615)
                     /locus_tag="OO315_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892898.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1619..1834)
                     /locus_tag="OO315_004245"
     CDS             complement(1619..1834)
                     /locus_tag="OO315_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5892899.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
BASE COUNT          544 a          564 c          468 g          548 t
ORIGIN      
        1 accgagaacg tttcgtccag caactggaga gagttcgacg gtggcatcat tgcccatgcc
       61 ggaggcgctg gaggtttcgg ctgaggatgg cacagggcat tttcctttga cgagcacccg
      121 accaccatta tcaagcttgc gccgaagagc atcattttca gctttcgcat cagctaactc
      181 cttcgtgtat ttagcatcga gtgcatcagc atcacgctgg cgctgctgca tgtcagtaat
      241 ggttgcgttc gccagcttca gttctccggc gtttttgtcg cgctgctctt tgtaggtaat
      301 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
      361 agcggagata atcgcggtga ctctgctcat tgatctatcc cccaacaggc taatgcgctt
      421 tcctggtcac gacgaataac ctgtccatag cagttatttg aacgtatgcg gcaatcgcgc
      481 ccaacatctt ttatccacca gcgaatcgcc tcgcatgcgc ccttacgatc accggcattc
      541 agccgcttat aaaacgtcga cgggaaacac ttaccggggc caatgttata ggggcaaaat
      601 gacgctatac ccgctttctg gggttcggtc agaggtactt taatattgcg ctccacccat
      661 gccagcgcct tatcacgttc aatagcgtta acctggtcac atttttcctt cgacagcttc
      721 attcccggta tgacaggctt accatccacc attgtggcac cacgacagat ggtccatatg
      781 ccagaaccat cacggtatgc cgttgtgtga ttaccctctt tttcgttcag aaactggtca
      841 agtatctggg gagcagacgc gcctgcggca atcagcgcca gaacagcagc cgacaggccg
      901 tatttgattt ttgcgctcat ggatatttat caggatttat cggcaacaaa taacgagcca
      961 acttatatac gccttttaag ataagtcagc cccggctgga tccagtcatc aggctctttc
     1021 ttaaaggggg agtattgaaa tcacgaagaa gagcctcccg caccgcagca tccatgtctg
     1081 caccactggc aagtgcttca atctctgccg ccacctgcaa ataccccatg caacggccaa
     1141 tgcgtttcat aactctggtg ttttcgtctt tcaggcatct gatagcgaga ttaataattt
     1201 tcaggcagtg atgatgcgat gcccggacag gttttccgtt ctcataaacg acgaactcac
     1261 cctcttcaca ggaaaccctg aaaggccgat ggggttcctg gtctttatcc ggtgcagacg
     1321 caataaaatc acttttatgc tcttcctctc cggcactgtt aaccgtaatg acgtactcag
     1381 gccgcaggta agccgcctct ttttctgaca atgcggccgg taaaaacttt tcctgcaaaa
     1441 acttcataaa agtctcgtga gcgactttgt ggcagtaatc gtaaatcgcc gcataatgct
     1501 catcgcggcg tttgttttca tcttcagaac tcaccaacgc cgacaatttt ttattaagct
     1561 cagtgatttc attttccagg tgattgaatc gctgattcat ttcttcatgc ttcattattt
     1621 actctccccg tgcagcctta cgcttgtctt ctcttatttt gaaatacaga ttcgtcagat
     1681 aagtcagaag ccccagaagc agacttccca gcacaccaat cgcagcccac tgtgatggcc
     1741 tgacctgatc caaccactgc aaaaaccagt agccggcact gccggcggag gtgccgtagg
     1801 caatgcccgt tgaaattttg tccatggatt tcatagcctc acctccgcaa ataacggatg
     1861 gcgtagtttt acactgagaa atgaaaggga tttgaaaaga aaaaaacgca aaagcgggcg
     1921 aaacgatata tacagtaagg aaagcactct atccaacaaa ccacccacag ttaatcggaa
     1981 taaaagcaga gtgcttatga atgatcgcac gcccgaaggt tagtatttct gcacagcaat
     2041 tttgcaaaaa aaggcgatca ttcataactt aaacgtcttt cagtcactcc gggatttccc
     2101 atcatcgcag actgaaagac tctg
//
LOCUS       DAJTRD010000242         2124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTRD010000242 DAJTRD010000000
VERSION     DAJTRD010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.245"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            42..221
                     /locus_tag="OO315_004246"
     CDS             42..221
                     /locus_tag="OO315_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892900.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            273..467
                     /locus_tag="OO315_004247"
     CDS             273..467
                     /locus_tag="OO315_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892901.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            complement(1266..1602)
                     /locus_tag="OO315_004248"
                     /pseudo
     CDS             complement(1266..1602)
                     /locus_tag="OO315_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          681 a          438 c          436 g          569 t
ORIGIN      
        1 agcgtaaggc actacagcgc attgctttac gagtaataac aatggattta tcaccagaag
       61 acgcaaagtt tttacgagaa ctgcaacacc gtatgctcag agaagccatt atcgaacagc
      121 aaacgggcat gagcgccata gacccattgc gatacaaacg ggcctcatct cctttagttg
      181 acgacaattc caatgcggat aaaacacgtc caccacactg attacatttt tcattagcac
      241 agtggtgtga tgcagcatac gaggaaaacc ggatgaatga gaacaaaata aaaaggctgg
      301 agcaactgtt acaggcacgc caacgggagt ttgccactaa atggggacgt gccgcgctgc
      361 cacatgaacg cctaacctat tttccacttg aagacctcat taaaaataat ggcaaaaaaa
      421 tcaggtctga taagaaacac ttcttagaat gcaatgaaga caaataacaa caatctgcat
      481 attgccacca accacagatg tggcatcgtt atcagcgcac ttgattttct ttatattcac
      541 tgacgagctt gccggggcac ctttgtttgc cggtcattct ggtatcatgc agatatttac
      601 ggcgtacaag cccactagca tgacattgta ataaggcctg agcctaacgc tttaatgtgt
      661 gtcatacact cagtggcgta atcgttgaca atatggtgca tgtaatcgta agcgtccagc
      721 gaaatccatc tggtcataat ctgaagcatc cgacaaagtg gtgtccacca aataagtgat
      781 ctgcgcccaa tatcctgagc cggaactttg acaaaggcca acgaaaaatg ggagcggatg
      841 ttacagagtt ctcggtacag gggaaaagtg gtacctgtcg ccagttctcc gatctgttta
      901 accgggaaat tatctcctac agcttgtcag aaaggtcggt gatggagcat cgttaatacg
      961 atgctatacg atgcattcac agtgcccggc cagaggatgc cccgtcgctg catatggatc
     1021 agggttggca atatcgaatc gcaggctata ggcaaagtta aagccccatg gagtagcaca
     1081 aaatatgccg cacaaaggaa acggtctgaa taacgcagtg atgaagaact tcttcagcac
     1141 actgctaaaa cgcatagtga tcgagcgctg attctggcga acaactgaac taatacatca
     1201 gaatctgcat tatgttaaat aaatatgaaa agatggttta aataccccgt tacttttgac
     1261 ttacactata cggtatcgca tcgtttaata ttcgcaccgg ccagattttt atttctatta
     1321 gttgtcacaa tactgaatgc gtacgaccac agtattctgg ctcctgtgtg gttatgcttt
     1381 aattctgcgt tccgggcaga taagcagttg cttgcaggaa tccttcttgt gttaatgtca
     1441 gttccccttt ttaccagtgc tgatttccac attccgtcca acagagctta tagcctttcc
     1501 ctggattata gcattgtccg gctgaagttc tttttgaata ataatagaag cactgctggc
     1561 agatccagtc cgtttttcat aacccactgt actgataacc ataatctaat cagtagaaat
     1621 tgagtcgaaa ataagcacta ctctatacag gataattaga ggtcagtttg attattcaca
     1681 attcatcatc agcattttct atttctgacg aaatcaacat gaaaataacc atatatgata
     1741 attattataa taacggcttt aattggaata catatattac aacgtattat atataattgg
     1801 tattctggga actatattct caaaatacag tagaaacgag gtatgtttct ggtggaaagg
     1861 acagtgggat taaaaagtaa gaattgataa aaaaaacgcc agcaacacat agtgccggcg
     1921 gagggaatac cccatggaga aaatgtgatg cctagagcat catgaatata taattaaaaa
     1981 tagttagcgt tgtcaatact ttcacaaaaa taattttcgt agtatagaaa gatattttta
     2041 tgcatgacct acctgaattt gctccgggta gaggttataa ataaaaattg aatcacgaca
     2101 aacacaatat tcatagtatg gcga
//
LOCUS       DAJTRD010000243         2121 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTRD010000243 DAJTRD010000000
VERSION     DAJTRD010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.246"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            376..519
                     /locus_tag="OO315_004249"
                     /pseudo
     CDS             376..519
                     /locus_tag="OO315_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            666..932
                     /gene="ospE1"
                     /locus_tag="OO315_004250"
     CDS             666..932
                     /gene="ospE1"
                     /locus_tag="OO315_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCR5892902.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1323..>2121
                     /gene="ipaH1.4"
                     /locus_tag="OO315_004251"
     CDS             1323..>2121
                     /gene="ipaH1.4"
                     /locus_tag="OO315_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HCR5892903.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGP"
BASE COUNT          704 a          451 c          361 g          605 t
ORIGIN      
        1 gcttcttacc atatttgaaa taaagctcaa tagcgcgaat tttttgttct tcagtaaaca
       61 tgtttcatct tctgagagtc caggaaatcg tccgcatccc cattaccgtc ttatatcact
      121 ggcgctgaca tgtgctcatt gatgaagtga atctgaatga catgttttgc catatcaacg
      181 ccaaccggtg tatatttcat ttgtggagtc tccagtctga ggacgcttta tgcatcccgt
      241 attgggcagt atgatgccgg aaagctgcga ggctccacac ctgccagttt caccctttta
      301 accatcatac tactccgtgt ttaattgtgt gggatgcgtt cattacattt taacagtgtc
      361 accccgtcgg ccacgaaaga taaacaggtg accagaaaag ggatcttcat ccagcacatg
      421 ctatatcttg aacgtaacaa cctgaccctc cgcacccgca tcaagcggct ggcacgtaaa
      481 acaatctgct tctcgcgttc cgtggaaatc cacgaaaagt catcggctcc ttcattgaaa
      541 aacacatatt ctactgattg gaagcgccac ccgaaaaagt atcgtttttt tacagtaaac
      601 ttcatttagc cgactataat gtaaaaaaat aaatttttta ccaaaatata acataaggaa
      661 gtaatatgct tacacaaact atattccctt gtcttccaca aaaacaagaa aatatcatcc
      721 ttgaagtatc aaatcctgta ttattatctt ctactgttac tacagatgga tatactgttt
      781 tcaataaaaa agcagctata tacgaattac aaatacctgc tgccagccgg acgaaaacgt
      841 tgaaattcac tgctacagaa atgcaatggt tgacaaagat aaatgaagcc ggaattgatg
      901 aaaaacaaag ccaacggtat tctgatttct gaagttaatt tcagactcgt ttaagctcca
      961 ttttcaaaaa aaatatatca gggtttgtta ctaataactt ctgaaaacag aaaaaaaata
     1021 gagcagtaat tctatttcag agaggagtaa ctccatctat cctcgatacg tactgaacgt
     1081 tatcgtgata tattcatcat gctttcttca ccattattcg agtataggga gagacgcaat
     1141 gaccttaaat acctttttat ggctgaatta ccattctcag gaataacagc agactccttc
     1201 ccatagacgg cttaggggga aagcaatacc cggagaaagg gtacaaagca aactgtttta
     1261 aaattaataa gccatattct atttaactag aattcttatc gttgataaga attctagtta
     1321 taatgattaa atcaaccaat atacaggcaa tcggttctgg cattatgcat caaataaaca
     1381 atgtatactc gttaactcca ttatctttac ctatggaact gactccatct tgtaatgaat
     1441 tttatttaaa aacctggagc gaatgggaaa agaacggtac cccaggcgag caacgcaata
     1501 tcgccttcaa taggctgaaa atatgtttac aaaatcaaga ggcagaatta aatttatctg
     1561 agttagattt aaaaacatta ccagatttac cgcctcagat aacaacactg gaaataagaa
     1621 aaaacctatt aacacatctc cctgatttac caccaatgct taaggtaata catgctcaat
     1681 ttaatcaact ggaaagctta cctgccttac ccgagacgtt agaagagctt aatgcgggtg
     1741 ataacaagat aaaagaatta ccatttcttc ctgaaaatct aactcattta cgggttcata
     1801 ataaccgatt gcatattctg ccactattgc caccggaact aaaattactg gtagtttctg
     1861 gaaacagatt agacagcatt cccccctttc cagataagct tgaagggctg gctctggcta
     1921 ataattttat agaacaacta ccggaattac cttttagtat gaacagggct gtgctaatga
     1981 ataataatct gacaacactt ccggaaagtg tcctgagatt agctcagaat gccttcgtaa
     2041 atgttgcagg taatccattg tctggccata ccatgcgtac actacaacaa ataaccaccg
     2101 gaccagatta ttctggtcct c
//
LOCUS       DAJTRD010000244         2094 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTRD010000244 DAJTRD010000000
VERSION     DAJTRD010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.247"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..776
                     /locus_tag="OO315_004252"
     CDS             join(<1..274,274..776)
                     /locus_tag="OO315_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR5892904.1"
                     /translation="TPRAGRPWAEHRKIINGMFWVLCSSAPWRDLPERYGAWKTVYNR
                     FNRWSKSGVINIIFNRLLSLLDANGFIDWSATALDGSNIRALKCAAGGSKKHPDIDGD
                     NGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAHESQFAQRLLDGIGVQRQNGSMKR
                     RGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVER
                     CFGRLKEYRRIATRYDKTARNYLAIVKLGCIRLFYQRLRN"
     gene            complement(946..1695)
                     /locus_tag="OO315_004253"
     CDS             complement(946..1695)
                     /locus_tag="OO315_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5892905.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          618 a          449 c          480 g          547 t
ORIGIN      
        1 acaccacggg ccggacgccc atgggctgag caccgtaaaa tcatcaatgg catgttctgg
       61 gtgttatgtt ccagtgcacc atggcgcgat ttacccgaac gatatggggc atggaaaact
      121 gtttataacc gctttaaccg atggtcaaag tcaggcgtga ttaatattat tttcaacagg
      181 ttgctttcgc tacttgatgc aaacggcttt attgactggt ctgccaccgc gctggatggc
      241 agtaatatcc gggcgctgaa atgtgccgcc ggtgctcaaa aaaacatccc gatatcgacg
      301 gagataatgg gccgggtcgc tctcgcggcg gttttggcac caaaatccat ctggcaacag
      361 acggaagtgg cctcccgtta aatatcgtgc taagccccgg acaagctcat gaaagccagt
      421 tcgcgcaacg acttctggac gggattggcg ttcagcgtca gaacggcagc atgaaacgcc
      481 gtggacatgc ggtactggct gataaagcgt attcagggcg cgcattgcgc aatgagctga
      541 aaaataacgg tataaaggca gtaatccccc gaaagtcaaa tgagaaaatg gcatcggatg
      601 gacgtgcaca gcttgatcgt gatgcgtact gcaatcgtaa tgtcgttgaa cggtgctttg
      661 ggcggctgaa agaatatcgc cgcatcgcca cgcgttacga caaaacggcg agaaattacc
      721 tggcgatagt gaaactgggc tgcatccgac tcttttatca acgcttacgt aattaaggga
      781 cacagcctag tgaaattacc gacaagaagg gaaagggcct ttccgagagt ggtaaaggag
      841 aggcactgga aataccccac agccctgaaa aagagccagt cagttgctta actgactggc
      901 attagccctg aaggtcgggg ttataaggcg tactggctaa aagcattatt ttttctgatt
      961 gttagcgaat tcatctttcg ctttttgtaa ttgtgcctgg aacacaggat ttgagtgcag
     1021 tgttgctaca atagcagagc caacaatacg tgctgcgtca acatcacttt gccagtgata
     1081 accgcaaata accctgctgt cccccagttc ataaccccgt ttcagaatgg tatcctggcg
     1141 tgccggattt atttcggaca atacaagtgc tgttgcccaa ccaatcgatg tatgacctga
     1201 tggataagag ccatttctgg ataatgtatc ctgttctttt gtattacacg tagaaacacc
     1261 ataaaaagca aaaggtcgaa tacgcatata atattctttt gctgaacgcg ttgctaggtc
     1321 tccggcatcc tcaatcatat ttgtcagtaa tttatgcaat tctggtgaat ctttagccgt
     1381 tatcggagaa ccaaaagcag cagaaaatac atttgccacg ccaccactac tgagattggc
     1441 atcctcagct gccagcttcc ctctttcagt atttcgcaat aaacgcccct tttcatacat
     1501 ggcctggtca ttcaggaagg caatacttcc gatttgtggt ggaggcggta ataatgccag
     1561 actgtcaata gcattatcgt ttgtcaggta gtaaaggtca ggttttgttg tcacatcatt
     1621 tcccggaggg aatgatgcaa aggtatttaa agaaaatacc ccgacaatac taagagtaaa
     1681 aagctgtctt ttcatataac atctccttat agaggataca tatttataat tattttatta
     1741 agagcataaa gtactggata ataaaattgt gtgtactatc acataattta aataaatcag
     1801 aagatgctaa aacaaaggtt acctattaaa tatccgttat gaacaagcat aaaaaggagg
     1861 acgtttaaaa cacccagaac aaacagttag aattaagttc ttaccaacaa ggggaataag
     1921 caacttgtat ttttgagttg cttttacaga caatgactcc aattagtaga acatatgttt
     1981 ttcgataaac gcaccgataa ctttttgagg tagcctgagt ttaacggaca ctccttcctg
     2041 aaatagaatg gcatcagaag gagccaatat ttacgggtaa agccctgttt atgg
//
LOCUS       DAJTRD010000245         2093 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTRD010000245 DAJTRD010000000
VERSION     DAJTRD010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.248"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            117..1154
                     /gene="curA"
                     /locus_tag="OO315_004254"
     CDS             117..1154
                     /gene="curA"
                     /locus_tag="OO315_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5892906.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            1352..2017
                     /gene="mcbR"
                     /locus_tag="OO315_004255"
     CDS             1352..2017
                     /gene="mcbR"
                     /locus_tag="OO315_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5892907.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          578 a          444 c          559 g          512 t
ORIGIN      
        1 ggattcctcg atgaaattat tgcgtgttgc agaggggatg tgacggctgc aaacaaaacc
       61 aatcccgtga gcctgctact attgatggat tcatgccaaa agcacaggag agcattatgg
      121 ggcaacaaaa gcagcgtaat cgacgttggg ttctggcctc gcgtccacat ggcgcaccgg
      181 ttccggagaa tttccgtctt gaagaagatg atgtcgccac accgggtgaa ggacaggtgt
      241 tactgcgcac agtttatttg tccctggacc cgtatatgcg tggacgtatg agcgatgagc
      301 catcttattc accgccggtt gatattggcg gcgtgatggt cggcggtacg gtgagccgtg
      361 tcgtggagtc gaatcatcct gattatcagc ctggcgactg ggtgctgggc tacagtggat
      421 ggcaagacta tgacatatcc agtggtgatg acctggtgaa acttggcgat catccgcaaa
      481 atccatcgtg gtcgctgggt gtgctgggga tgccaggctt taccgcttat atggggctgc
      541 tggatatcgg tcagcctaaa gagggcgaaa cgttggtggt agctgcggcg acagaaccag
      601 tgggggcgac ggtggggcaa atcggcaaac ttaaaggttg cagagtggtg ggggtagccg
      661 gtggcgcgga aaaatgccgc catgctaccg aggtgttagg cttcgatgtt tgccttgatc
      721 accacgcgga cgattttgcc gaacaactgg cgaaagcgtg cccaaaaggt attgatatct
      781 attatgaaaa cgtgggcggt aaggtatttg atgcagtgct accattactt aatacatctg
      841 cgcgcattcc cgtttgtggc ttagtgagca gctataacgc tacagagcta ccacccggtc
      901 cggatcgttt acccctgttg atggcaacgg tgctgaaaaa acgtattcgc ttgcaagggt
      961 ttattatcgc tcaggattat ggtcaccgca tccatgagtt tcagaaggag atggggcaat
     1021 gggtgaaaga ggataaaatc cactaccgcg aagatattac tgatggttta gagaatgcgc
     1081 cacagacgtt tatcggcctg ctgaagggta aaaacttcgg caaagtggtg atccgcgtcg
     1141 cgggtgatga ttaaagtttc aacggcggcg taagccgcca taaaaatcca ggcaaaaagc
     1201 ttattttcag ctttaattaa ctaaccacgt cattgttctg ctgctaataa caacaaaggt
     1261 gggtggttta tgattattca ctttaataca ctaggtgaac tccttctgcc atgcaggcag
     1321 ggttggacag aaaacacgcg aaggaattaa aatgcctgga acgggaaaaa tgaaacatgt
     1381 cagtttgact ctgcaggttg agaacgacct gaaacatcag cttagtattg gcgcattaaa
     1441 acctggcgca cgcctgatta ctaaaaatct ggcggagcaa ttaggtatga gtattacacc
     1501 tgtgcgtgaa gcattattac gtctggtttc ggtgaatgcg ctttctgtcg cacctgcaca
     1561 agcatttaca gtaccggaag tgaggcaaag tcagttagac gaaatcaatc ggatccgcta
     1621 cgaactggaa ttaatggcag ttgccctggc tgttgaaaac ctctcctcgc aagacatagc
     1681 agaacttcag gaattgctcg ggaaattaca gcaggcgcaa gaaaagggcg acatggaaca
     1741 aatcattaat gtgaacaggc tatttcgctt agcgatttat catcgctcaa atatgcccat
     1801 cctgtgtgag atgattgagc aactgtggat caggatggga cctgctttac attatcttta
     1861 tgaagcgatt aacccagcgg aattacggga gcgcatagaa aactatcatc tattactcgc
     1921 tgcgttaaaa gcaaaagaca aagagggatg caaacattgt cttgctgaaa ttatgcaaca
     1981 aaatattgct attttatacc agcaatacaa tcgttaaatt aaatgcccgg caaataccgg
     2041 gcatcaagta attactcaaa tcgccacgca atattcatgc cgacaccata att
//
LOCUS       DAJTRD010000246         2076 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTRD010000246 DAJTRD010000000
VERSION     DAJTRD010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.249"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(216..1865)
                     /locus_tag="OO315_004256"
     CDS             complement(216..1865)
                     /locus_tag="OO315_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5892908.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          501 a          547 c          528 g          500 t
ORIGIN      
        1 cccagagaag tcagtgggtt acgcagagaa tcgtaacggg ataccagatc aagagcctgt
       61 ccaatgtgca taaaaaaatc cggaaacaag tgagcgtttc cggattctta cacagccact
      121 ggatcggtca actgatcctt aactgatcgg cattactcct cctgaagagg ataattttac
      181 agccagcagg aagttagcgg ctggctgtaa aaatattatg gttcgccgta caaaccaatt
      241 aaacggcgta ccacaccgtt agtccagcca aacccatcct gcaacggata ctcgccaccg
      301 ccgccttcgc ggggaacacc atcggcaata tggtatttct cgatcatttt gtgctgttcc
      361 agatagaact gattcaccgt tttcagccag ctgcgcgcga tttcatcacc cagaaggtca
      421 tcgccataca ttttaaatcc ctgaattgcc atccattgta acggtgccca gccgttgggt
      481 ttatcccact gttcaccggt ttcgtactcg cttgccaaaa tcccgccagg tgtcagtaac
      541 cggctgcgta cggcgtttgc cagacgatcg gcctgttcat ggttcgccat gccgacatag
      601 agcggcacaa tggcggcagc ggaaaacagc gccagttgtt cgcgtcgcca gtcgtaatcg
      661 cggtagatgc cgttttcatc atcccagagg taacggttta ccgcatcgcg acgggcactg
      721 gccttctggc ggaacagcgc ttctgtctct ttctcgcctt tcagcgccga gatgttggcg
      781 atggcgctct ccagtttaaa caggaaggca ttcagatcga tggggatgaa ctgggtggta
      841 cgaatgctcg ccagacgacc agtatcacgc agccaacggg aagagtaatc ccaaccggag
      901 gccgctcccg cgcgtaaatc gcggtacacc tcgttgggcg ggcgaccaga atgtttcgcg
      961 gtttcaacgt cctcaagcca ggattcgtca cgcggcgtgt cgcgatcgtc ccagtaacgg
     1021 ttgagcagcg atccgtccgg catccgcaca acatggcgat aggcctgatt agggatcaac
     1081 gattctgcac cgtccatcca gaaggcatat tccattttaa ggtggtcgag atagcggcgc
     1141 gcaccgcgta caccatcttc ttcaaacaac tccaccatca gcgcaaaaac cggtggttgc
     1201 gagcggctca aataataggt gcggttgccg ttggggatgt gaccgtagtt ttcgatcatc
     1261 caggcgaagt tatcggccat gcatttcagc aaatcttccc gaccactttc cgccagtccc
     1321 agcatggtga aataggaatc ccaatagtag gtttcgctaa aacggccgcc cgggacaata
     1381 tatgactgcg gtagcgccag cagagaagac cacggaatgt gatcctgtgg ttcgcgggtt
     1441 agcaccggcc acagctggtc gatatgctct ttcagggaat tttgcgggtc cgatacatac
     1501 tcgctggagt agacctccgg cagccagaag tgattttcaa caaacttgcg caagtcaaaa
     1561 tcacgatgac ggcgcacttt acggtagcgg attaaaatat ccagcgggtc cattttgggt
     1621 gcgcagtcgg gaaaggtttt actgtcgggg aaaattttcg ccgactgaac atgctcgaac
     1681 agttcgagat agcgatcggc aggcgtcagc gcatcagagg cgggcagccc ttcaatcatc
     1741 tcgggttccg gttctgcctc gatcatctca tccagtttta attcatacgg gtccagttca
     1801 tagcagagat cgacctcgat catcagttcg tctggattag ggttttgaat tttctgattg
     1861 agcataagga tccaacctcc gaaattcgtc gctaattatc gggcttttct ttaagggtag
     1921 tcattcgctc cgcctggtgg gcgacaaagc gtgcggtgga tcacgtttat taacttgtca
     1981 aaaaatataa cgcactaata tcaatggaat ttataaaaat acggatttgg tgttgttttt
     2041 cattttcaga acattcaatc ctgctctcgc cgcaat
//
LOCUS       DAJTRD010000247         2049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTRD010000247 DAJTRD010000000
VERSION     DAJTRD010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.250"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            240..509
                     /locus_tag="OO315_004257"
                     /pseudo
     CDS             240..509
                     /locus_tag="OO315_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="OO315_004258"
                     /pseudo
     CDS             506..732
                     /locus_tag="OO315_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="OO315_004259"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="OO315_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR5892909.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
     gene            1690..1974
                     /locus_tag="OO315_004260"
                     /pseudo
     CDS             1690..1974
                     /locus_tag="OO315_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          580 a          450 c          482 g          537 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaacgtaac cggctcattt
     1621 aaaccgtctg gtctgttacc tccggttcca caaaaataat gtccatcatt ttgaagtgga
     1681 cactatcgta tggaataccg gacctgaatc actgaagctt tacgccttca tttcgaagaa
     1741 catttaccac gggttgtggc cggatgtcgc ctgggcgtac caaaatcaac tgtctgtggt
     1801 atgttcgtgc gcttccgcaa cgctggcctt tcatggcctc tgcccgcagg tatgtcggag
     1861 caggaacttg atgcccttct ttacggaagt gcctccacag tacctgtcgt acttactgaa
     1921 agcaccgtta tgcccaaact cccggtggta aaaaaacgtc cccggcggcc taatgccgat
     1981 cagttaagga tcagttgacc gatccagtgg ctgtgtaaga atccggaaac gctcacttgt
     2041 ttccggatt
//
LOCUS       DAJTRD010000248         2045 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTRD010000248 DAJTRD010000000
VERSION     DAJTRD010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.251"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            6..224
                     /locus_tag="OO315_004261"
     CDS             6..224
                     /locus_tag="OO315_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5892910.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO315_004262"
     CDS             226..591
                     /locus_tag="OO315_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5892911.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="OO315_004263"
     CDS             588..1253
                     /locus_tag="OO315_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892912.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OO315_004264"
     CDS             1253..1618
                     /locus_tag="OO315_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5892913.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          558 a          423 c          542 g          522 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttac
//
LOCUS       DAJTRD010000249         2029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTRD010000249 DAJTRD010000000
VERSION     DAJTRD010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.252"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..1824)
                     /locus_tag="OO315_004265"
     CDS             complement(<1..1824)
                     /locus_tag="OO315_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5892914.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREAELTLVNGVHGHGKACPLNEPILLADGTWTTHGNV
                     KIGDQVASVDGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGFTKGEK
                     RRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSNGSVKF
                     SNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTAKNKFI
                     PRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
     gene            complement(1932..>2029)
                     /locus_tag="OO315_004266"
     CDS             complement(1932..>2029)
                     /locus_tag="OO315_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5892915.1"
                     /translation="WNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
BASE COUNT          485 a          579 c          430 g          535 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt ctactgacgc cacctgatcg ccaattttta cattcccgtg
      661 agtagtccat gtcccatcag ctaaaagaat aggctcattc agtgggcatg cctttccgtg
      721 accgtgcaca ccattgacca gcgtcaactc tgcctcacgg aactggaatt tatctgccag
      781 agattcccac ggtggattaa acagatactg ctgcttgccg tagaaagcgt tgatagtgtc
      841 ctggtaaaac tctcgcgcac tgtagagttc ttcaggatcg aagtaggatg ccgtgccgat
      901 gtactgccag atttcatcct cggtaacacc gttcatcagg cattcgttga tgtctttgta
      961 cggcagagta acaagacggc aacgatgttc accgagtcgg cttacgattt cccttgcggc
     1021 ttcacgacca acatcatcaa cgtccatcga gatgaatatt tcctcaaacc tgtcgaggtt
     1081 gtgatactca aactcaatcc actgttgctt agcgcctttc ccgccaccaa acggcacgga
     1141 taacgccgag atgccgtatt gcgcatagct catacaatca atttcgcctt cgcaaagcac
     1201 aaccaccctc acgccagcgt ccagagcctg ccatccgaac aggcaaggtt cgcaatcacc
     1261 ttctgccata atgactttct tcccgtccgg gcgctcagtg ctgattcgct tgacctgcaa
     1321 caactcacca tcgcgtttgt acggaagcac cagagcatcc agttctcgtt ctccattcca
     1381 caccttgccg ctgacaacct cgtagcgctt tacgacttct ggcgatatgc cacgcgattg
     1441 caggtactca agatgggatt ctgttctggt aacgtagcgg gcgattttct tacgatcagg
     1501 tctggagaat ttcttctcac gtctggcatc gaaatggtga tcgtcatcct tgataccgag
     1561 aaatgctttc gcttcctgca tagcctgatg caggttaatt ccacgacatg ccatccacaa
     1621 atcaagcatg tcaccgccgt ctccctcagc gaaatcagcc cattttttct tgccgataag
     1681 gttgacctta aggctgtttc ccttgtcacc gttgacgtta ccggcaaccc actcatgccc
     1741 ctctatcttg ccgtttggca acaggtgcgg agccaccctg tcaacctgcg cccaaagcag
     1801 gtcgctaagt tcacttggcc tcatgattcc ctcagattga gatttttaaa ccagaaatcg
     1861 acaaacgaaa tacttaacca gccgtggtta taaccagcga ccagtagcga tttgattttt
     1921 gatttcatgg ttcacctgtc gaaaaacaca tagccagttt tcgatacggt gattgcggat
     1981 gatggtctgg attgtggttg aatggtttct ggcttctcgt cgttccagc
//
LOCUS       DAJTRD010000250         2019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTRD010000250 DAJTRD010000000
VERSION     DAJTRD010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.253"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..2019
                     /gene="sat"
                     /locus_tag="OO315_004267"
                     /pseudo
     CDS             1..2019
                     /gene="sat"
                     /locus_tag="OO315_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          743 a          352 c          396 g          528 t
ORIGIN      
        1 acggcatgat gatttataaa aatgataaaa catttcgtaa tcttgaaata ttcggagaca
       61 gcggctctgg agcatactta tatgataaca aactagaaaa atgggtatta gtcggaacaa
      121 cccatggtat tgccagcgtt aatggtgacc aactgacatg gataacaaaa tacaatgata
      181 aactggttag tgagttaaaa gatacctata gtcataaaat aaatctgaat ggcaataatg
      241 taaccattaa aaacacagat ataacattac accaaaacaa tgcagatacc actggtactc
      301 aagaaaaaat aactaaagac aaagatattg tgttcacaaa tgggggaaat gttctgttta
      361 aggataattt ggattttggt tgcggtggta ttatctttga cgaaggccat gaatataaca
      421 taaacggtca gagatttaca tttaaaggag caggaattga tatcggaaaa gaaagcattg
      481 taaactggaa tgcattgtat tccagtgatg atgttttaca caaaataggc cccggtactc
      541 tgaatgttca aaaaaaacag ggggcaaata taaagatagg tgaaggaaat gttattctta
      601 atgaagaagg aacatttaac aatatatacc ttgcaagcgg aaatggtaag gtaatactaa
      661 ataaagataa ttcccttggc aatgatcaat atgcggggat attttttact aaacgtggtg
      721 gcacgctaga tttaaatgga cacaatcaga cttttactag aattgccgcc actgacgatg
      781 gaacaacaat aactaactca gatacaaaga aagaagccgt tctggcaatc aataacgaag
      841 actcctacat atatcatggg aacataaatg gcaatataaa actaacgcac aatattaatt
      901 ctcaggataa gaaaactaat gcaaaattaa ttctggatgg tagtgtcaac acaaaaaatg
      961 atgttgaagt cagtaatgcc agtcttacca tgtaaggcca tgcaacagag catgcaatat
     1021 tcagaagcac agcgagtcat tgctccctgg tatttctttg tggaacggac tgggtcaccg
     1081 ttttgaaaga aacagagagt tcatataata aaaagttcaa ttctgattac aaaagtaata
     1141 atcagcagac ctcatttgat cagcctgact ggaaaaccgg ggtgtttaaa tttgatacat
     1201 tacacctgaa caatgctgac ttttcaatat cacgcaatgc caatgttgaa ggaaatatat
     1261 cagcaaataa atcagctatc acaatcggcg ataaaaatgc ttacattgat aatcttgcag
     1321 ggaaaaatat tactaataat ggttttgact tcaaacaaac tatcagtact aatctatcca
     1381 taggagaaac taaatttaca ggtggcatca ctgcacataa cagccaaata gccataggtg
     1441 atcaagctgt agttacactt aatggtgcaa cctttctgaa taatactcct ataagtatag
     1501 ataaaggagc aaaagttata gcacaaaatt ccatgttcac aacaaaaggt attgatatct
     1561 ccggtgaact gactatgatg ggaatccctg aacagaatag taaaactgta acgccgggtc
     1621 tccactacgc tgctgatgga ttcaggctga gtggtggaaa tgcaaatttc attgccagaa
     1681 atatggcatc tgtcaccgga aatatttatg ctgatgatgc agcaaccatt actctgggac
     1741 agcctgaaac tgaaacaccg actatatcgt ctgcttatca ggcatgggca gagactcttt
     1801 tgtatggctt tgataccgcc tatcgaggcg caataacagc ccccaaagct acagttagca
     1861 tgaataatgc gatctggcat ctaaatagcc agtcatcaat taatcgtcta gaaacaaaag
     1921 acagtatggt gcgttttact ggtgacaatg ggaagtttac aacccttaca gtggacaacc
     1981 ttactataga tgacagtgca tttgtgctgc gtgcaaatc
//
LOCUS       DAJTRD010000251         1929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTRD010000251 DAJTRD010000000
VERSION     DAJTRD010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.254"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            274..578
                     /locus_tag="OO315_004268"
                     /pseudo
     CDS             274..578
                     /locus_tag="OO315_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004952983.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
     gene            747..>1929
                     /locus_tag="OO315_004269"
     CDS             747..>1929
                     /locus_tag="OO315_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5892916.1"
                     /translation="MSLFTEPKEIERLPSEEIERLYPVLRYRVFISIFLGYMGYYFVR
                     NTTSVLSGVLHMSATEIGIISCAGFLSYGISKFVSGLISDRSNSKVFLSLGLFLSGLV
                     NFLIGYIPGIITSVTLFSTMYLLNGWIQGMGYPPGAKTLVFWYEHRERITWATLWNLS
                     HNVGGALAPVLIGFSFGFFGDSALDHARAAFIFPGVLCMAMSVLIYFIQVDRPVSVGL
                     PPIEEWKGNVVSHPAKGREQGPRLSIPDIIRKHIIRNNKLIYCCIYGSFVYILRYGIV
                     SWAPKFLSDSLDVGGKDMGKLASMGGGSVFEIGGVAGMLLAGYLSVRLFRNSKPLTNT
                     LFLALTIILLIAYWYVPSGNEYLWLNYTILILLGLAVYGPVMFIGLYSMELVPKEAAG
                     AA"
BASE COUNT          508 a          380 c          439 g          602 t
ORIGIN      
        1 gggttgggga gcaatggaac agtaaacgcc gttaagaagt caatttaaat agccaaaaat
       61 taactagcca atttacattg caggttgtaa tttttaacca ccttttttac gatagccaaa
      121 ttacctatac ccatcccggc cctgccaagc catacccgct atcggcgatg aaaatgcttt
      181 tgtgcgcggt cggagtggtc ccgacgaggg ttttacccga agtcggggcg tatctccgcg
      241 ttagcgggcc gtgagggccg cttacgagcg tgtatgagag acgtacagtg gttttgtccg
      301 ctcacaccag tacatacagg tacggaaata aaaccagtcg aaatgccggt aacgacgatt
      361 actcctgtct ctgatgccgg tggactacaa gattttattt actggcgtcc tgatgcggac
      421 agggctggta tggaacctgt ttatgtgata cttaatgatc ctctggattc cgggcgattt
      481 acgcgtaaac aactaaataa aaatatctta aacatgcaag taattttggt attctcgata
      541 caagaataaa tagtagtaac tactatagct gcaaataatg ttaaaaacat ttctatgatc
      601 atctttacat ttttcatgat tgacaattgt ttttggttaa aaaaagacaa ctaatgagtg
      661 tgataatgca atactttgag tgattatata atgataagta aacaggtttg cgacaattgg
      721 ttatcagtat tacagaagga ttaaatatgt cattattcac agaacctaag gaaattgaaa
      781 gattaccctc tgaagagatt gaacgattat atcccgtcct gcgatacagg gtctttatca
      841 gcattttcct cggatatatg gggtattatt ttgtccgaaa caccaccagt gtgttatccg
      901 gtgtgctgca catgtctgca accgagatcg gcattatctc ctgtgccggt tttttatcct
      961 atggtatatc aaagtttgtt tccggactta tttctgacag aagtaattca aaagtatttt
     1021 tgtctcttgg tttattcctg tccggactgg taaattttct gataggatat attcccggga
     1081 tcatcacatc cgttacgtta ttttcaacaa tgtatttact taacggctgg atccaaggta
     1141 tgggatatcc tcctggtgcc aaaacactgg tgttctggta tgaacatcgg gaaaggatca
     1201 cctgggccac actgtggaat ttatcgcaca atgtcggggg agctctggcc cccgttctga
     1261 ttggtttcag ttttggtttt ttcggcgaca gtgctctgga tcatgcccgg gccgcattta
     1321 tcttcccagg agtactgtgt atggcaatga gtgtgctgat ttactttatt caggtggaca
     1381 gacctgtatc agtggggctt cctcccattg aagaatggaa aggtaacgtc gtttcacacc
     1441 cggctaaagg ccgtgaacaa ggtccgagac tctccattcc tgatatcatc agaaaacata
     1501 tcatccgaaa taataaactc atttactgct gtatctatgg ttcgtttgtt tatattctga
     1561 gatacggtat tgtttcctgg gctcctaagt ttttaagtga cagtctggat gtcggtggta
     1621 aggatatggg aaaactggca tcaatggggg ggggttctgt ttttgaaatc ggtggtgtgg
     1681 ctggtatgct tcttgccgga tacctgtctg tcagactgtt cagaaactca aaaccattaa
     1741 caaatacttt atttctggca cttaccatta ttttattaat cgcgtactgg tacgtgccat
     1801 caggcaatga atatctctgg ctaaactata caatccttat tctgttgggg ctggcagtgt
     1861 atggtccggt gatgtttatt ggtctttatt caatggagct tgttcctaaa gaagctgccg
     1921 gagccgcat
//
LOCUS       DAJTRD010000252         1921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTRD010000252 DAJTRD010000000
VERSION     DAJTRD010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.255"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(30..1181)
                     /locus_tag="OO315_004270"
                     /pseudo
     CDS             complement(30..1181)
                     /locus_tag="OO315_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1246..1894
                     /locus_tag="OO315_004271"
                     /pseudo
     CDS             1246..1894
                     /locus_tag="OO315_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          478 a          482 c          422 g          539 t
ORIGIN      
        1 gatcctaccc acgtaatgtg gacacggccc taagcgagat tatggttttc aaattgttcc
       61 gggctgagac cgccacaggc actgtgacga cgccagcgat tgtaatcaca ctcgatataa
      121 ttaaacaccg ttgcccgcat tatttcccgg ctgctaaagc gttccccgtg gatacattcc
      181 accttcagcg aatgaaagaa gctttccaca caggcattgt cataacagta gcctttcgca
      241 ctcatactgc cacgcaggtt gtgtcgcttc agcattgcct gataatcccc tgaacagtat
      301 tgaccaccac ggtccgtatg aacaatgacg ctttccgggc gtcttctccg ccacaacgcc
      361 atttgcagtg catcacaggc cagttgtgct gtcattcgcg gtgacatcga ccagccaatg
      421 acggcgcgtg accacaggtc gatgactact gcgagataca accagccctc atcggtacgc
      481 aagtacgtga tgtcacccgc ccacttctgg tttcgggcca ctggcgctga agtcctgctc
      541 cagcagattc tccaatacgg gcaggccatg tgcacggtag ctgaccgggc tgaacttccg
      601 gctggctttc gcccgcagcc cctgacgacg caggctggcg gcaatggttt taatattgaa
      661 ctccggcagt tcgtcagcaa ggcggggagc accgtatcgc tgctttgcct caaagaatgc
      721 cttatggaca gcggcatcgc aggtgagccg aaactgttgg cgcaggctca tctggtgacg
      781 acgcctgagc cagacatacc agccgctgcg ggcaacccga agtacacgac acatcgcttt
      841 gatgctgaac tctgcccgat gattttcgat aaagacatac ttcatttcag gcgcttcgcg
      901 aagtatgtcg cggccttttg gaggatggac agttcctcag cctgctccgc cagttgtcgt
      961 ttaaggcgga cattttcagc ggccagttcg ctttcgcgct ctgacgaact catttgttgc
     1021 tgctgtttac tgcgccaggc ataaagctga gattcataca ggctgagttc acgggctgcg
     1081 gcggccacac cgatgcgttc agcgagtttc agggcttcgt tacgaaattc aggcgtatgt
     1141 tgtttacggg gcttcttgct gattgatact ggttttgtca tgagtcacct ctggttgaga
     1201 gtttactcac ttagtcctgt gtccactatt ggtgggtaag atcaccctcc attaaaacta
     1261 aaacgggcgc tttctgatat atcatctgta agcgcagtaa taccattagt agcacctcgc
     1321 gcattgatta ttgaatatta taactggaat gttatatttc ttgttatgat gctgttcagt
     1381 attataataa tgttattatc accaaagata actcatgcaa atgagaaagt aatatgtcaa
     1441 aacagtgcat cctttgtgac caaagagttt atctgcggca ctatacttgt ctcattaata
     1501 ctcagttctc tattttgcta catatcaata tcaccatcaa ttttcatgca tacttatggc
     1561 ctgagtactt ttagttactc aattattttc tccttctcag ttttattttt cataatcgga
     1621 aatcaattat caaagattga aaaattatcc aacctatggt ttttattacc caatagccaa
     1681 ttttatcgca ttacaaataa atggttcaaa aacaggattt gccgcatcta ttacaggttt
     1741 tactcaaaca ataagcgctg gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg
     1801 tgttggtccc ggaactactt taggtcttgg ttcactaata acttcattac tgtcaatagc
     1861 cgtaatcgcc attgcaggaa ataataaaaa gtaagtgtac tctgactgcg tatgtatcta
     1921 c
//
LOCUS       DAJTRD010000253         1910 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTRD010000253 DAJTRD010000000
VERSION     DAJTRD010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.256"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            353..1711
                     /locus_tag="OO315_004272"
     CDS             353..1711
                     /locus_tag="OO315_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR5892917.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
BASE COUNT          505 a          473 c          514 g          418 t
ORIGIN      
        1 gggatatggc cctgcctttc cagcaatcgt cgattgttat accagtccac ccacgtgagt
       61 gtggtcagct catcatactg acacgtgcat tacccctacc gcgataatgc aaccgtacaa
      121 tatagggcgt ttcctaaacg cagccctcat cactccaatc caacaagaca cagcattttt
      181 gatcacgaag aaagacaaat aatttccgta aatgtttccg ttgttacaaa taacaacagt
      241 gttttacatt tctttggcct caggcccgcg catgttgcca ttcgatgtca ttttcctgac
      301 tcgtttcttc tttatggtgt agtgtgatct tcactttata gatgctaaca atatgaaaag
      361 aaaaattatt cccgtgctaa tcggttgtac gctctctttc tctgccctgg cggcacaacc
      421 tacggctgag cgctatgtcg tcagttttcc agaaggcacg cacgtgaact acgccggcgc
      481 atttgccagt gcattcccga acggccttcc ggttgggata ggttctggcc tgctgttcac
      541 cggtaaacag ggcgatgccc tgacattcgc aaccattacc gatcgcggtc cgaacgcgga
      601 ttcaccgaaa gaggggaaaa acgaaactaa aattttcgtt acgccagatt tcgcgccgct
      661 gctgatgacg attcgtgtac aaaacggcaa agcggaggcc atcgatcccc gtcctctgca
      721 tgacgataaa ggcgcgatca acggtctgcc gctggcaagc gacgtgattg gctccaccaa
      781 tgaagtggcc ttcagcgaca cgttacatag actgaagggg gataatcgcg ggctggatac
      841 ggaagggatc actccggatg gtaaaggagg ttactggctg tgcgatgagt atggcccgtt
      901 cctgattaac atcgacagta aagggaaaat cctggcaatc cacggtccgc aggcggcgga
      961 aggggaaaaa gcgattgcgg gcggtttgcc aaatatcctc aaatggcgtc aggcgaaccg
     1021 cggctttgaa ggcctcaccc gtatgccgga cgggcgtatt attgtcgccg tgcaaagtac
     1081 gctggatatc gacgctaaga gcaagaaaaa ggcgctgttt acgcgtctgg tgagcttcga
     1141 tccggcaagc ggaaaaaccg cgatgtacgg ttatcctatc gacagcgcgg cctacagcaa
     1201 aaacagcgac gctaaaatcg gagacatcgt ggcgctcgat aaccagcaca tcctgctgat
     1261 cgaacaggga agagataaaa acaacagaat gcgtaacctg atttacgagg tcgatctcaa
     1321 taaggcgagc gacctgtcag gcttcgacaa gccgggtgag tatccggagt ttgatgatga
     1381 gaaaaccctg tcacagcgcg gcattaccct tgcgcagaaa acgcaggttg ttgatctgcg
     1441 ctccttgggc tggcagcagg agaaggctga agggttggcg ctgatcgaca gcaaaacgct
     1501 ggcggtggcg aacgataacg actttggcgt gaaggtggcg atgcaacatc cggtcgaggg
     1561 caagacattc aaggattatc gggtgaacgc ggaaggaaag ctgacgcttg acgacaagca
     1621 ggttgaaacg accctcagag tgaagccact ggagaaaccg gagtctgaca gtgaactgtg
     1681 gattgtgacg ctgccggaag cgttgaaata agtttataag gtacggacag cagtatcgct
     1741 tagtttttca aattcatgta tgtgaacaat attggggttc cggcgtagac ccccaatgaa
     1801 agcgtcacca cgtgaaaaca tgcctgcacg gtttacacgt catcccatgg tggcagccgg
     1861 ttaactttcc tgcgccacca gtggaaaaag gccataagca gtccgaacaa
//
LOCUS       DAJTRD010000254         1871 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTRD010000254 DAJTRD010000000
VERSION     DAJTRD010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.258"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..259)
                     /locus_tag="OO315_004273"
     CDS             complement(<1..259)
                     /locus_tag="OO315_004273"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892918.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARC"
     gene            complement(1012..1485)
                     /locus_tag="OO315_004274"
                     /pseudo
     CDS             complement(1012..1485)
                     /locus_tag="OO315_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1413..1779)
                     /locus_tag="OO315_004275"
                     /pseudo
     CDS             complement(1413..1779)
                     /locus_tag="OO315_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          429 a          525 c          442 g          475 t
ORIGIN      
        1 tgcagcgggc aatttccgga tacccttcgt catcaacacg tacaaaccag aagaccagtt
       61 ttttgtttcc ggcatccaca aagaacggaa tattcaggtc tgcgcaacac tcaacggcat
      121 cgtcaaaatt atcaaagcgc agaacctcag cttcttcttc gtcaaaaaaa tcatcttcgt
      181 gaagttttac gacatagcgg aggagtttgc ttctttgagg tgcaggtttt cgtttgcgtg
      241 gtttagctga acgggccata taactacctg aaagacaatg atattgtctg tttttataac
      301 ggtaatcaca ggccatgaca agccgcagcc gtcaggccgc ctactcgggg cggtttagaa
      361 cgcttattta gtaccagatc ttacttataa taaaggggct tggcagtttg gataaactat
      421 tgtccttatt ccactaatac aaactttttg taaagtatat taaatatact tcatttcata
      481 ctgaaatccc cggaagtatt caatcaaaca attacaccat aaaaggaaga gggggcccca
      541 attaagttgt caagcatgtt tactgcgcaa tcgcttggtg ctccgtccct gtaccccgca
      601 ttggtactct ggcgctcatg gggcttccac ttgcgcttct cccttcacat cggggtgaca
      661 ggtccacgtt ccccacaaga gcctgaatcg acttcacgcc gcttttatgc cggatgccgc
      721 gcaggcagta aacatatccc acaggaacga ctccaccatt gttttgacat catctatatg
      781 ctttcgacac atcatcagcg gttcactttc gttcgtctct tcgaaccata cctgatcggc
      841 tcacgtccga ccttttccca tctcgctcac caccacatct cttaaatgca gcagcagtgg
      901 atggtttggt gcctgcgcct gcacgccgac accaaggggc cttccctcat ctcttgtgct
      961 gcatggctgc acagggactc acgtccccag gacgcgcctt cgtggcacac agtcatcggc
     1021 atagcaacag taagcaacgg caggtttcca ttgccagttt tctttgacag cagtacttcg
     1081 gcctcgctgg atactgtgat tccagtacca gcgatccttt ctggcttttc cactcaggta
     1141 gcgtttattc agatactggt cgaactcatt cagtatgatg ttcgacagca acggcgagat
     1201 cacaccgccc tgtggcatac tttcactgac cgcccggaag agaccaacat caacacggcg
     1261 ttttccacag cagtctcata aaacgtgtat cactgatcct gcggcagacg gctttcatca
     1321 gcagccggtg atgtactgtg tcgaagtagc tggacaggtc accttcaata acccggcgcc
     1381 ccgtgtttcc ccgcagtcgg tgagctgtaa tagtacgtga tgtctcccgc ccacttctgg
     1441 ttcgggccac tggcgtaaaa atcctgcgtc agcagatttt ctgatacagg caggccgtgt
     1501 gcacggtaac tgatcgggct gaacttctgg gaggcttttg cgctcagttc ctgacggcgc
     1561 agactggctg ccacggtttt tacgttaaag tggtaaccag agcacacagt tcatccgtca
     1621 ggcgtggggc accgtaacgc tggactgggt aaaagccttg cggacaacgc tgtcgcagtg
     1681 ctgacggaac tgcggacgtg tgtttatcct tgtccgccgc tgacaccacg tataccagcc
     1741 gctgcgggcc acccggagta cgcggcacat tgccttgatg acggtttacg cggtcgcctg
     1801 cggcaacggc tttccggctg acggtagtgt ggaccttttt accccggaga acaccggcaa
     1861 gccccataac c
//
LOCUS       DAJTRD010000255         1821 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTRD010000255 DAJTRD010000000
VERSION     DAJTRD010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.259"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(203..604)
                     /locus_tag="OO315_004276"
                     /pseudo
     CDS             complement(203..604)
                     /locus_tag="OO315_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1033..1821
                     /gene="virF"
                     /locus_tag="OO315_004277"
     CDS             1033..1821
                     /gene="virF"
                     /locus_tag="OO315_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5892919.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          568 a          329 c          338 g          586 t
ORIGIN      
        1 gaatatagtt tgtgtaagca tattgcttcc ttatgttata ttttagtaaa gaatttattt
       61 tttacattat agtcggctaa atgaagttta ctgtaaaaaa acgatacttt tttcgtaagc
      121 gatgagatgt ggagccagaa aaatggctgc gttacgtcat tgaactgtcc ttgacccacg
      181 atattcagac agtcgttgta tcttaagttg acattacccg ctgacgtcga tattcccttg
      241 gtgagagata acccaacgca ctatgcggat ggttttcatt gtaatgctcg atcgccactg
      301 caaggttatg caatgccgtt cttggtttcg gcatgaacgc gatgcagtct tccttcatcg
      361 ttttcatgaa ccgttctgct attccattac tctgagggct actgactgcc gtcgtgcagg
      421 gctccagatt caactctctg gcgaactgcc gtgtttcatg agccgtataa gccgaaccgt
      481 tatcggccac tggattggcc gttccggtcc tttatcgcca tatcgctcct gacatgacat
      541 cctgcaccgt tgctttgtca taatctcccg tgcctgctgc ccagtctatg gcttcacgat
      601 cgcaatatgg aacgtaacgc tgtcatcggg agaatcgtca atgcggcatt cggttaacgc
      661 ttaccaatat caacaccaac cggatttgtg gagcctccag tctgaagggg ctttatgcgt
      721 tccgtatagg atattatgat gctggagttt tgcgaagctc cactcctttc cgaacattta
      781 cacagatatt gcctaagaaa agtgaaacca taatatggtt atagtccctt tcagtgcaaa
      841 tacttagctt gttgcacaga gaaatagaag ctgcataagc tctttcttca aaaaatgtaa
      901 ataaagttaa atataggaaa aattacttaa tctatcttaa taacggaaag attttgtata
      961 caatcactgt tacagaaatt tcttagttac tctgtaaaca ctaaatatag tttggtatat
     1021 tctgttgaat ttatgatgga tatgggacat aaaaacaaaa tagatataaa ggttcgcttg
     1081 cataactata ttattttata tgcaaaaagg tgttcaatga cggttagctc aggcaatgaa
     1141 actttgacta tcgatgaagg gcaaattgct tttatagagc gaaatataca aataaacgtc
     1201 tccataaaaa aatctgatag cattaatcca tttgagatta taagccttga cagaaattta
     1261 ttattaagca ttattagaat aatggaacca atttattcat ttcaacactc ctattctgag
     1321 gagaaaaggg ggttaaacaa aaaaatattc ctcctctctg aggaggaggt ttctatcgat
     1381 ttgttcaaat ctataaaaga gatgcctttc ggcaaaagaa agatctatag tttagcttgc
     1441 cttttatcag ctgtttctga tgaggaagct ttatatactt cgatatcgat agcttcttct
     1501 cttagttttt ctgatcagat aaggaagatt gttgaaaaaa acatcgagaa gagatggcgt
     1561 ctttctgata tttcaaataa cttgaattta tcagaaatag ctgttagaaa acgattggag
     1621 agtgaaaaat taacatttca acaaatcctt cttgatattc gcatgcatca tgcagcaaag
     1681 cttttattga atagtcaaag ctatattaat gatgtatcaa gacttatcgg aatatcaagc
     1741 ccatcttatt ttataaggaa atttaatgaa tattatggta taactccaaa gaaattttac
     1801 ttatatcata aaaaatttta a
//
LOCUS       DAJTRD010000256         1809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTRD010000256 DAJTRD010000000
VERSION     DAJTRD010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.260"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(92..1696)
                     /locus_tag="OO315_004278"
     CDS             complement(92..1696)
                     /locus_tag="OO315_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5892920.1"
                     /translation="MIQLSSSHKLPAVYYLYQRNGVYYFRLRVRQSNNDRMTSISLRT
                     KDRRTAMAYSRHIKAALKAIHADRPNATYEEMREHLKDIAECELSMGRSDLFEPDMRD
                     IYRDQYGELGESLTDALASEPLSIDQHRYINEALKVLKACMRRIEAGDSQPLIDYVDL
                     FNDIDRQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWKPATLRENKA
                     SHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADLLSREDKTDC
                     LDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARNAFSTEQVGQ
                     LLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAGTVYIHINED
                     DSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMVFDGLRLMKN
                     GYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQAIMGHSSGS
                     ITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          449 a          456 c          410 g          494 t
ORIGIN      
        1 ttaattatcc cttgacaagt agagaaaact atgagtcaaa aacatgacca cacaggtctg
       61 tagtaagtgt catcctagac tatggtctcc tctaggtctc cttataaaca tcagccatca
      121 cattgaccag cctatcaacc tcgacctctt tagcataacg gtcgtacgta atagaccctg
      181 aactatgccc catgatcgcc tgtgcatacg ccaactcgac cccatgttgt tttaactggg
      241 ccgcaactgt atgtctaaac gagtgaaacg ctaagccgct acggtctacg aggactttag
      301 gcaacaacgt ccgattaaac catttactca cctgctcacc gtaaccgttc ttcatgagac
      361 gcagaccatc gaacaccata gcgtcatcac cgtcagcgcc tctacgggtc tctacaagcg
      421 ccataaagtc agccaatata aaaccatagg ccccatcgac cagaggcaca cagcggtcac
      481 tatgagcgtt cttgatgctc ttaccgggta gactactatc gtcttcattg atatgaatat
      541 aaacggttcc cgcctcagtg gtcctgacgt ctttgacctg tagttgggct atttcattga
      601 gtctcgcccc agtgatagca gccaaggcgg taacataata atgatacggc ttaccagagg
      661 tcttctgaga gtatgcctta gctgcgacca gtagttgccc cacctgctcc gtagagaagg
      721 cgttacgggc ccctgaggct ttacgctgag ggactttaag ctccagaccc tcagtcatgt
      781 tctttttaat caagtcattg cgcacagccc acctaaagac agcagccatc ttaataagat
      841 acttgttgtt gatggtaacg acatccagac aatctgtctt atcttcacgg ctcaacaggt
      901 cagcaagtgg gacatccttg aagcgctgct tacggttcct aggtaactgc tggagcacat
      961 cacggaccct aagcatgtca gccctgttag cgtctgcatt gagacccaga tggtcgaata
     1021 tttcgattaa cgcagcgtgt gacgccttgt tttcccttaa ggttgcaggc ttccagttct
     1081 gatagttctc cgcttcgtat tgttcaaaga ggctcgctat ggtaatgcta ggcgtaactt
     1141 ctggttttac ttctggagca ttaacagata gggacacaga gtctgcttgg ttatcttgcc
     1201 tatcaatatc attaaaaaga tcgacgtaat ctatcagagg ctgactgtct cccgcttcga
     1261 tacgcctcat acaggctttt agcaccttca gggcctcatt gatgtaacga tgctggtcaa
     1321 tgcttagcgg ctcactagcc agagcatcgg taaggctctc accaagttct ccgtattggt
     1381 cccggtagat atccctcatg tcaggctcaa agagatctga acggcccata gacagttcac
     1441 actcagcgat gtccttaagg tgttccctca tttcctcata ggtagcgttg ggtctgtctg
     1501 cgtgtattgc cttgagtgct gcctttatgt gccttgagta agccatagcg gtgcgtctat
     1561 ccttggtccg taatgatatt gaggtcattc tatcattgtt tgattgtctg accctaagac
     1621 ggaagtagta gacgccgtta cgctggtaga gatagtagac tgctggaagt ttgtgactac
     1681 tggatagctg aatcatatgt ctgcatgccc tgagagggtt acacagtggg ttacagtaag
     1741 taactctgca aggaggctta aagccgtatc ccttaggatt cagtaggttg gagtacttta
     1801 agaaaggtc
//
LOCUS       DAJTRD010000257         1779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTRD010000257 DAJTRD010000000
VERSION     DAJTRD010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.261"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..360
                     /locus_tag="OO315_004279"
     CDS             <1..360
                     /locus_tag="OO315_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5892921.1"
                     /translation="PPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKCM
                     TCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLLL
                     IRFLMHARNSGVLKWKA"
     gene            361..726
                     /locus_tag="OO315_004280"
     CDS             361..726
                     /locus_tag="OO315_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5892922.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            723..1406
                     /locus_tag="OO315_004281"
     CDS             723..1406
                     /locus_tag="OO315_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5892923.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPVEFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
     gene            1605..1680
                     /locus_tag="OO315_004282"
     tRNA            1605..1680
                     /locus_tag="OO315_004282"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1638..1640,aa:Ile,seq:cat)
     gene            1682..1758
                     /locus_tag="OO315_004283"
     tRNA            1682..1758
                     /locus_tag="OO315_004283"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1716..1718,aa:Lys,seq:ttt)
BASE COUNT          490 a          376 c          492 g          421 t
ORIGIN      
        1 ccaccacagg aagagacggt ggtcagcatt gccgttgatc cggaatctcc ggctcagtat
       61 ctccagcgcc agaaaccaca acgggaagag atgcctgtat acacgcgctg ggtaaaaacg
      121 cagaaatgca tgacgtgtgg caatcaggca gatgatccgc accacatcat tggtcatgga
      181 ctgggaggga tgggaacaaa ggctgacgat ttgtttgtta ttccgctgtg tcgtaaatgc
      241 cataacgaac tacacgccgg ggtaaaagat tttgaagaaa aacacggtag tcagttgttg
      301 ttgctgattc gttttttaat gcacgcgaga aattcgggtg ttctgaagtg gaaagcataa
      361 atgaccgaac gcatagaatt tgttttgcct tacccgccaa cggtgaacac ctactggcga
      421 cgtcgtggca gcacatattt tgtatcaaaa gccggtgagc gttatcgccg tgatgtggcg
      481 ctaattgttc gccagcagcg actgaaatta aacctgtccg gaaggctggc gataaagatt
      541 attgcagagc caccggataa gcgccgtcgt gacctggaca atatcctgaa ggcaccgctg
      601 gatgcgctga cgcatgctgg actacttata gacgacgagc agtttgatga aatcaatatt
      661 gtgcgcggtc agctcgttcc tggtgggcgg ctggggataa aaatcacaga actggggtgc
      721 gcatgaataa ccagtattta cagtttgtgc gtgagcagct cattatcgcc accgctgatt
      781 tgagtggggc aacaaaaggt cagcttgaag cctggcaaga gaatgccatg ttcgatacag
      841 ggcgttacag gcgtaaaaaa atccggtacc gcgatgaagt gactggaaaa atgataacgc
      901 gggataatcc accaatcccg ggaaagcaat cgctggcgaa ggggacgtca attcctctgg
      961 tcagtccggt tgagttttcg acatcatcgt ggcggcgggc tgttctgtct cttgaagagc
     1021 actataaagc ctggttgttg tggtgttaca gcgggagtat ttgttgggaa tatcagatcg
     1081 cgataacaca gtgggcgtgg aatgaattta atgctcaatc cggtaccaga aaaattgcag
     1141 ggaaaacgca ggaacgcctg aaaaaattaa tctggctggc ggcgcaggca gtaaaagcag
     1201 aactttttgg tggggaaggt tatgaatacc aggagctggc attactggcg ggagtgacaa
     1261 ctaaaaactg gtccaaaaca tttactcgtc actgggttgc aatgaaacac atttttcacc
     1321 gactggatag tgaggcttta ttgtttgtaa tgagaacacg ttcaaaaaaa agacggcatt
     1381 ttcaaagcaa agtgttgcaa aagtagattg aaaggcatat attcatgcaa atctgatact
     1441 ttgccgattt tgtacgtgat ggcaaaagca aacaaaaccc gcccacaagc gggttttttg
     1501 tgccacttat ctcggataga catggtgaat gcgctggtgg aggagataag ggtgattttt
     1561 gaatgcttgc aacattgatt tcgtaacgtt attatcctgc gcccggccct ttagctcagt
     1621 ggtgagagcg agcgactcat aatcgccagg tcgctggttc aaatccagca agggccacca
     1681 accgccacta gctcatcagg aaagagcgtc aaccctttaa gttgagtgtg cgaggttcga
     1741 gtccccggtg gcggtccagt gccgacttgg ctcagtagg
//
LOCUS       DAJTRD010000258         1761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTRD010000258 DAJTRD010000000
VERSION     DAJTRD010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.262"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(1..265)
                     /locus_tag="OO315_004284"
                     /pseudo
     CDS             complement(1..265)
                     /locus_tag="OO315_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OO315_004285"
     CDS             complement(380..1018)
                     /gene="ospI"
                     /locus_tag="OO315_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5892924.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1177..1647)
                     /locus_tag="OO315_004286"
                     /pseudo
     CDS             complement(1177..1647)
                     /locus_tag="OO315_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          502 a          435 c          319 g          505 t
ORIGIN      
        1 ccagcaagcg ctgtaactct tttatttgtt ttatagcggc agccagttct gaggcaggaa
       61 cgacatcctc tccggcagca acagcagtga gacttccttc cagatattat tttttccact
      121 taagcaacag gctgggctga ataccatgca aacgcgccac atgggacacc gtcatgccag
      181 gctccattgt ctgctgaata atggcaattt tttcctgagg agttttacgt ttacgaagct
      241 ccagccccag caaagttcca gtcatctcaa aattagaact agtgttagac atatatccaa
      301 gcctatctct tattttaaga tacatccact gtctggcgtt tcaaggggcc ggctcaaatc
      361 tatgtacagg ctctcagcat cagcaaagcc tcttactttt ccaactacta ctgttagcat
      421 agtgatcaaa ttcctgatca gatacatgaa ttacctgaac cgaactattt tcatctacaa
      481 atgcatctgg cgatttttct acaggatcat aaagattaaa tccctttcct gtttgaacat
      541 ccaaaaacat aattttttca ccatcgttaa atccagctat ccaatgagtg gcctgttcaa
      601 tagaaatcat tactacactt ccttttgaag tattttgtaa aatattttct gcaatacgct
      661 cacgaaattt actataagtt tcattaggat gtgacactat gccttcacta tcaatttgct
      721 ctaaatgcct atgaaaaaat ccggtcatat actctgatat ttcattaggt acgggagcct
      781 ctcttactcc ataccctaac atagccatac aagcatgaag cgcacaccct gaacagttac
      841 catccccacc agacggattt atcattttct gtaaagtcgc tgccaattca gaatctttat
      901 ccatatcacc aagactttta gagtttaaag attccagcaa ttttttaaca tttacatttc
      961 cctcctctgt atgtgcctca tatccagcag ttccctgtaa cgacacccca ttaatcatat
     1021 tgtatcctga cagtatatat actcactata ttttcttcat aacaaatatc cctctgatat
     1081 gacaacaaaa aacggagccg gattcctgtt tttgtgaact gcagctattc gtgtctaaaa
     1141 agtgatccac ttaccaccaa cggcctaata tatacagaac ggagtaaaca ggactaacag
     1201 ttcccgacag gtctcatcat tccgtggccc gaaagtgtaa gccaacacac cgccagtttt
     1261 agtgttgtac gaataccaga gccagtgttg ttgcagcctt gctgccaacg aagcgccact
     1321 gctcatcaag ttcacagata agggcaacat cagcgtgagc gactggcgca gatgttatcc
     1381 gccggggcgg gagcttttta atgtccgtat gacggtattg atgccgattt tcagtgtcct
     1441 ggcggtatca cggccccctg cgccgttatg tgccatttca acgatctgct ccttaacgcc
     1501 gggttttctg gcttcataag agtaagtgag ctggaaaaca cgtttacatg agcgacaacg
     1561 gaaacgctca tgccgggaac aactacggcc atgtcgataa acttcatcgg aatgacagcg
     1621 cggacaatga acagtaacgg tagccatgga aaaccttaaa taccagtaga ttaccagaaa
     1681 cttctgtcga ttgaagacat cacccgaagt ttgaattcat tcgaatactt tttacgaggt
     1741 tcttttcgcc agtcctgtaa t
//
LOCUS       DAJTRD010000259         1717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTRD010000259 DAJTRD010000000
VERSION     DAJTRD010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.265"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(110..577)
                     /locus_tag="OO315_004287"
     CDS             complement(110..577)
                     /locus_tag="OO315_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5892925.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OO315_004288"
     CDS             complement(643..882)
                     /locus_tag="OO315_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5892926.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="OO315_004289"
     CDS             complement(1017..1622)
                     /locus_tag="OO315_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5892927.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          378 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAJTRD010000260         1686 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTRD010000260 DAJTRD010000000
VERSION     DAJTRD010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.266"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..285)
                     /locus_tag="OO315_004290"
     CDS             complement(<1..285)
                     /locus_tag="OO315_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5892928.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGT"
     gene            423..1343
                     /locus_tag="OO315_004291"
     CDS             423..1343
                     /locus_tag="OO315_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5892929.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1343..1686
                     /locus_tag="OO315_004292"
                     /pseudo
     CDS             1343..1686
                     /locus_tag="OO315_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          459 a          395 c          415 g          417 t
ORIGIN      
        1 tgtcccggat gggcggttct gcagatcgct cagggatgtc agggcagaat ctatgtcatg
       61 caatataggg ccaagaacag acaaaagatg ctcgcccgct tcagtgggta caacgctgcg
      121 cgtggttcgc gtcagaagcc gcaatcccaa tcgttcttct atacggcgca ctatctggct
      181 taaagctgac tgcaccatgc tcaggcgggc tgctgcgcga gtgaagctac gctcctctgc
      241 aacgacgaca aacgccatca gatcagcgat ttcttcacgc ttcattcagt atctccatac
      301 aatctattaa tcatttaaat gataggtcta atcatgattt agggtctaat caacttaatg
      361 atcaatatct atagtgatat caccaaccgt caaaacgaac cgacaactcc atgaggaact
      421 gaatgatgaa taataaagtc agcttcacta acagcaataa tccaaccatc tctttgtctg
      481 cagtgatcta tttccctcca aaatttgatg aaacccgtca gtatccggca attgtggtat
      541 ctcacccagg tgggggcgtt aaagagcaaa cggccggaac ctatgccgaa aagctggcgg
      601 aaaagggatt tgtcactatt gcttatgacg catcttatca aggtgaaagt ggcggcgagc
      661 cgcgtcagtt agaaaaccca tacattcgta ctgaagacgt cagtgccgtg attgattacc
      721 tgacaacgct ttcttatgtc gacaataccc gaattggtgc gatgggaatt tgtgccggtg
      781 caggatatac agccaatgct gcaattcagg atcgccgcat caaggccatc ggtaccgtta
      841 gtgcggtcaa tattggttcg atgttccgca atggttggga aaacaatgtg aaatctattg
      901 atgctctgcc acacattgaa gctggctcaa acgcccgaac cagcgatatt agcagcgaag
      961 aatacgccat tatgccactg gctccaatga aggaggccga tgcgccgaac gaagaactgc
     1021 gtcaggcctg ggagtattac cacacacctc gtgcgcagta tccaacagca ccgggttacg
     1081 ctactttgcg cagccttaac cagattatta cctatgatgc ttaccatatg gcggaagtgt
     1141 acctgactca gccgatgcag attgtggcgg gcagccaggc agggagcaaa tggatgagtg
     1201 acgacctgta cgatcgggca tcaagccagg ataaacgcta tcacattgtt gaaggcgcaa
     1261 accatatgga tctttatgac ggcaaagcct atgtggctga agcaatttca gtattagctc
     1321 cgttctttga ggaaactctg taatgaaaca tattcaaatc cgcaatagtg atatggcctg
     1381 gcatattgct gccaatattc agtttccacc aaattttgat gagtcaaaac agtatccggc
     1441 gattatcagc gtccatcctt ttggcagttg taaggagcag acgtctggca atatctatgg
     1501 caaggcattg gctgaaaaag gctatgtcgt tctcgcttat gacgccagtt tccagggaga
     1561 atctggtggg tagcctcgtt ggatagaaga tccgacacag cgcgtagagg atattagccg
     1621 tgttatcgat tatgcagtaa ctctgccata tgttgatgct gaacgcattg gcgtgctggg
     1681 tgtgtg
//
LOCUS       DAJTRD010000261         1600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTRD010000261 DAJTRD010000000
VERSION     DAJTRD010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.267"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO315_004293"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO315_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5892930.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
     gene            complement(1261..>1600)
                     /locus_tag="OO315_004294"
     CDS             complement(1261..>1600)
                     /locus_tag="OO315_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5892931.1"
                     /translation="DLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRTEADRWAQAEE
                     QKYEMLENEYSQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQLTDEVLALRL
                     SENGSRLHHS"
BASE COUNT          419 a          339 c          358 g          484 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtcgtg agccgttttc agacaatcgc agggccagta cctcgtcagt
     1321 caactgacgg taaatctgct gttcagtctc acgcatcacc tgtgcaccgg cttccctctc
     1381 cgcatccgca tcaccgctca gacctgatgc tttcagccgg tcagccaccc tctgagagta
     1441 ctcattctcc agcatctcat acttctgctc ttctgcctgc gcccagcggt cagcttccgt
     1501 acgcttcagt acagcatgcc atggtcccca gagggagaac cagtccgtaa attcattctc
     1561 ttcacggctt ctgaccatgg cttcggcagt gcggaggtca
//
LOCUS       DAJTRD010000262         1591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTRD010000262 DAJTRD010000000
VERSION     DAJTRD010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.268"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(333..479)
                     /locus_tag="OO315_004295"
                     /pseudo
     CDS             complement(333..479)
                     /locus_tag="OO315_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            533..715
                     /locus_tag="OO315_004296"
     CDS             533..715
                     /locus_tag="OO315_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HCR5892932.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(816..1145)
                     /locus_tag="OO315_004297"
     CDS             complement(816..1145)
                     /locus_tag="OO315_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5892933.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1317..>1591)
                     /locus_tag="OO315_004298"
     CDS             complement(1317..>1591)
                     /locus_tag="OO315_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892934.1"
                     /translation="LNAQKLCDTQHMMQQILLSLVHALYEGNPQPVFANTEKLNDAVE
                     ELRQLLDNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK"
BASE COUNT          430 a          366 c          375 g          420 t
ORIGIN      
        1 agcaatgtct gtcgcacaac aaccacaatg tcacaaataa aaaatgagaa taaccagttc
       61 cctgaaggcg acgtagcccg gtacaagagt aacccacgtt agcgcttggc gacggcggaa
      121 caaagctgat tcgcgacgta agaaagtggc aggctcatat gacggacctc tgtaatgcgg
      181 tatccaaccc gcgaatatca gcatgattca ccgtcggatt tactgctacg tcgccctcag
      241 ggaattaaac ctaaaaagaa aatcgtcaaa attagagatt ccggtttgac acggaaagtc
      301 atatcagcgt caacggagca ccgtattgac gcttatttat tggtgagaac tacgttccat
      361 ggcaggagtt cgtcaacacg gttggagggc cattcaggca gtatgctcag aatatggcgc
      421 agatacgctt ccggatcgat accgttcaga cggcaggtgc ctgcttttcc ggcaacaagc
      481 aggaatctca gcgctgaagc aggaattaga ggtaaaaacg ccctaccggg ccatgaatca
      541 cccggtcatt ggagtagtaa ccaaagcaga tttagccagc atggaacaaa tatctttggt
      601 gaaaagttgg ttacgggaag ctggagcgca caacgtgtta gtaaccagtg cagttaataa
      661 caatggagtt acagaacttt ttgccttgct gcatacagaa gaaggctgtt gttaatttcg
      721 attggttctc agatgagaat gatgacggtg atacacagat aagaatcaat gagcaatgag
      781 ggttgccggg caaccctcat tgaataaaac gggaattact tcgcttcgcc gtttttcatt
      841 tcgcccatag ctttcagctt tttggagata tcgcggcgtt ctttggagag ctcggcattt
      901 ttgatgatgt aatcgtcaac gcgatcttca tagtcacctt tcatgctggc gatgatgccc
      961 tggattgctt caacgctcat ccctggcttg atgtaatcgc tcaggttgtc cagcaacagg
     1021 acgcgcttct ggttgtcacg gatctttttc tcaacgtcct gaatttcacg ttgcagtttg
     1081 ttcttacgac ggaacagacg aacaaattcc agtacgtcct ggaatgaagg cttggtagtt
     1141 tccattttta tacccctgat aatgtgagag tcggattcgt ttaagcaacc gctattcgtt
     1201 agggccaaca ttactcatag ctgccgcaga tgacaatgct tttatccctt ttcactatca
     1261 taccctttat ccttgctgaa tcgaagcagc agcaagatga ttctgaagtt caggaattat
     1321 ttgcgcaagg cccggcaaat cagattcgat agcaactcaa gctgatgtgc cgtttccatg
     1381 ttcagccaca cataaccata gattggtgtt tccacgactt tcaggtcatg gtggttatcg
     1441 agcaactgac gcagctcttc cacagcatcg ttcaattttt ccgtattggc aaaaaccggc
     1501 tgcggattac cttcgtacag cgcatgaaca aggctcagca gtatttgctg catcatgtgc
     1561 tgggtatcac aaagtttttg cgcgttcaat a
//
LOCUS       DAJTRD010000263         1587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTRD010000263 DAJTRD010000000
VERSION     DAJTRD010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.269"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..626)
                     /locus_tag="OO315_004299"
     CDS             complement(<1..626)
                     /locus_tag="OO315_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5892935.1"
                     /translation="MILSVFLGQPALTYAGVVIGSTRVVYLSNNADKSISVFSKEEKI
                     PYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEKILRIIHTKGVSLPDDRESVFWL
                     NIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAEN
                     PNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPANSAGGQMTWRFIN"
     gene            complement(740..1312)
                     /locus_tag="OO315_004300"
     CDS             complement(740..1312)
                     /locus_tag="OO315_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HCR5892936.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          472 a          334 c          316 g          465 t
ORIGIN      
        1 tcattaataa atcgccaggt catttggcct cccgcagaat tagcaggtaa aggtaacgtg
       61 agcgtttcaa aaggacggat catattgagg ggcacatcat ggccatcaac gatgacatcc
      121 atcacactga tatgaattgg gttgggattc tctgctatca gatttcgccc ttcccggcgc
      181 catttcactt tcggcgcagc atcttcggga ataaggcgga tatttgctgg tcgccagaac
      241 agcttgatac gcgtttttac tgctatttcc agcgagttcg tcgctttgtt gctcgctgat
      301 ggcggaatat ttttaatatt cagccagaag actgactcgc gatcatcagg caatgacaca
      361 cctttggtgt gaataatgcg taatattttt tcttgcgaag gttctaatcg tgatactggc
      421 ggaataacgg taaacggcgc tttgctttta tcctctttat taaatggatc tacccaagcc
      481 tggatgagat aaggtatttt ttcttctttg ctaaatacag aaatagattt atcagcatta
      541 ttacttaagt aaaccacccg cgtactacca atgacaacgc ctgcataagt taatgctggt
      601 tggcctaaaa aaacgctgag tataatcgcg gtaaataact tacattaaac tgttgtttta
      661 aagagcctga ttaaacaact ttttctggac ataacgactc cctggccaaa gccaactgaa
      721 taaaaaggcc cttgataaat tatttcttat attcgacagt aaattgtgaa tcagcgttcg
      781 ccgtaccagc cgttacggtc gcattggttg ccacatagcg tgcaataaac ttcatttcag
      841 ccttgccttc agcatcgata gcggtataag cagagtcgtt ataaagttta acctgcgaat
      901 tatcggactg gttaaaaata cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac
      961 cggtaaaatc accagggttg cctgctgtaa gcggcgtacc cactttcagg tcgccgtcgc
     1021 cgccaggcgc ttctgtacca tcaaaacgaa cggctgcttg agtgtacgtt gccgggcaat
     1081 tttcaagttt aatactgaac ttctgtggcg acgccagact acctacacct gaaaatgcat
     1141 ttttactgac tttacccagt gggacaattt gatcggctgt atcggaggta acttcacaag
     1201 tagtgtcgac aatttcaccg acgaagttaa caacaccatc ttctgccagt gcatgcccgg
     1261 ccagcattag agatgccaga gtgaatgcac cgcctataat attacgcttc ataaaaactc
     1321 cattgatata atatatcctt taccatgaat catcacttat attaataagt gtgtttttga
     1381 ttcaaaggaa tagtacacca aagagacggc tttatctaaa aataatatta attacgcaaa
     1441 aataaaatat agatttattg tttaaatatt atttaaacag taaaaaatca agtttgttat
     1501 cagacattaa tgataattca gaatatattt atatagaaag atagatgaat ttattgatgt
     1561 cagttataaa aaagggccac cggtcgg
//
LOCUS       DAJTRD010000264         1573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTRD010000264 DAJTRD010000000
VERSION     DAJTRD010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.270"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..1259)
                     /gene="yiaN"
                     /locus_tag="OO315_004301"
     CDS             complement(<1..1259)
                     /gene="yiaN"
                     /locus_tag="OO315_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HCR5892937.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLLKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGIMMGATLMLTWWRQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILP"
     gene            complement(1262..>1573)
                     /locus_tag="OO315_004302"
     CDS             complement(1262..>1573)
                     /locus_tag="OO315_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HCR5892938.1"
                     /translation="VAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWG
                     ATLKTIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQG
                     A"
BASE COUNT          478 a          399 c          406 g          290 t
ORIGIN      
        1 gggaggatga tgagatcggg aataaaaaca aatacgacta ataatgaata taaaaccagg
       61 acgtaaggga aaacgcctct gactgcatca tcgaatttga gttttgccac ccctgaaata
      121 acgttaagta cattaccgat aggcggtgta attaagccga ttgagcagtt aatgataaac
      181 atgacaccga aataaatcgg atcgattcct gcctctttaa ctaaaggcat taataccggg
      241 gtaagaatta ataccgtcgg cgttaaatcc atgaccatgc cgacaatcag gatagccacc
      301 ataatgacga taaacagcag acgcggtgaa tcgaccaacg gttgcagtaa atcagaaacc
      361 atcatcggca gttcagcaat ggtaatcagc caggcggaaa cttgtgccga ggccaccaga
      421 aacataacca ctgaggtggt tttcgccgcg ccaatgagta catgccaaag tgtggcgaag
      481 gtcatttcac ggtaaataac tgtggcgaca aacagcgcat agaaagcagc aacggccccg
      541 gcttcggtag gggtaaacag accggagcga aacccgccaa taatgatgac tggaagaaac
      601 agcgcccaga taccggagac aaaagagtgc cagatttcct gcatcgttgc tttttgctgg
      661 cggggtaaat taaggcgact ggcctgccgc caccaggtaa gcatcagcgt tgcgcccatc
      721 attatgccag gggcaatgcc cgccataaac agcttactga tggataatcc gctggaaaca
      781 ccgaagataa taaacggaat ggaaggggga ataattggcg cgataatgcc gccagaggca
      841 atcagccccg ccgcccggtt gaccgggtag ttggcgctgc gcatcatcgg caccagtaga
      901 gcggcaacgg cggcggtatc cgcaacggca gaaccagaaa ggctcgccat aatcatagct
      961 gccagcacgc cgacgtagcc cagcccgcct ggtttatgcc ccaccagttt catcggcagg
     1021 tcaacaatgc gttttaacaa gccgcccgca ttcatgattt cacccgccag cacaaagaac
     1081 ggaatcgcca ggagggagaa gctatcggct ccgttcacca gcgtttgtgc catgatctgg
     1141 acatcaaaca tgtccagcca gaacattaac gccgccccgc acaacaacag tgcccaggca
     1201 ataggcaaac cgatagcaat accacccaac agacagccca gaaaaatcag cacagccatg
     1261 attaagctcc ttgcggtggc gacgttagag aattgctacg cgtgatgagt tgatataaat
     1321 gacgcagttc aaagaatgcg ataacgaggc tggtgggcag acaagcggca tacatcaggc
     1381 cgatgggcaa accgaggatc ggtgaataat cgctccagtc ctgaattgtt tttagcgttg
     1441 cgccccaggc cagtgcgcca caaataaata agattaagga atgggtaacc agagcgactc
     1501 gtcgttgcca tgcgggggag agtttctcca ccagaaaggt gacctgaacg tgggcgttat
     1561 ccataaaagc cac
//
LOCUS       DAJTRD010000265         1555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTRD010000265 DAJTRD010000000
VERSION     DAJTRD010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.271"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            177..1202
                     /locus_tag="OO315_004303"
     CDS             177..1202
                     /locus_tag="OO315_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5892939.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVSNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            1270..>1555
                     /locus_tag="OO315_004304"
     CDS             1270..>1555
                     /locus_tag="OO315_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5892940.1"
                     /translation="MPHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFQNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLHNTLLVS"
BASE COUNT          377 a          365 c          400 g          413 t
ORIGIN      
        1 ttcgcaccat tgcggggcgc tgtttttatt atttcctgtc tacactcagc ttattgcgac
       61 gcgaaatcgt gcgcaaaagc tggcactact tttgcttata agaggatggc cacagacagg
      121 taaaagacgt ttcccaaacg tcctataacg ataattttcg cctcacagga agcattatga
      181 aaaagatgat gatagccaca ctggctgccg ccagcgtgct gcttgccgtt gcaaatcagg
      241 cgcatgctgg cgcgacgctt gatgccgttc agaaaaaagg ttttgtgcaa tgcgggatca
      301 gtgatggatt acctaggttc tcttatgccg atgcagacgg taagttttca ggtattgatg
      361 ttgatgtttg tcgtggtgtt gccgctgctg tatttggtga cgacacgaaa gtgaaatata
      421 ccccactcac agcaaaagaa cgcttcaccg ctttacagtc aggggaagtg gatttgctct
      481 cccgtaatac gacctggact tcatctcgcg atgccgggat gggaatggca tttaccggcg
      541 tcacttatta cgacggcatt ggcttcctga cgcacgataa agcggggcta aaaagcgcga
      601 aagaactgga tggcgctacc gtctgtattc aggcgggtac tgataccgaa ctcaacgtcg
      661 ccgactattt caaggcaaac aatatgaagt acacaccagt gactttcgat cgctctgacg
      721 aatcagcgaa ggcactggaa tctggtcgct gcgatacgct ggcttcggat cagtcgcaac
      781 tgtatgcctt gcgcatcaaa ttaagcaacc cggctgaatg gattgtctta ccggaggtta
      841 tctctaaaga accgcttggg ccggtagttc gtcgtggcga tgatgaatgg ttctcgattg
      901 tacgctggac gcttttcgcc atgctaaatg ctgaagagat gggcatcaat tcccagaacg
      961 tcgatgaaaa agcggctaat ccagcaacgc ctgatatggc acatctgttg ggtaaagaag
     1021 gcgattacgg caaggatctg aagttggata ataaatgggc ctataacatc atcaaacagg
     1081 tgagtaacta ctcggaaatt tttgagcgta acgtaggttc agaaagcccg ctgaaaatta
     1141 aacgtgggca aaataatctc tggaataacg gcggtattca gtacgcgccg cccgtgcgtt
     1201 aagtcggtgt gaggtaacga gcgtcgctac ggcggcgctc tcttcagcgt tatgctttcc
     1261 gaggtttcta tgcctcatcg ccgctcaacc gttaaaggct cactctcctt tgccaaccct
     1321 acggttcgtg cctggttatt ccagatcctt gccgttgttg ctgttgtcgg cattgttggt
     1381 tggttatttc aaaacactgt aacgaatctc aataatcgtg gcattacttc aggttttgcc
     1441 tttctggatc gcggcgctgg cttcggtatt gtccagcatt tgatcgatta ccagcagggc
     1501 gacacctacg gacgcgtttt tattgtcggc ttacacaata cgctactggt ttctg
//
LOCUS       DAJTRD010000266         1480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTRD010000266 DAJTRD010000000
VERSION     DAJTRD010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.272"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            206..457
                     /locus_tag="OO315_004305"
     CDS             206..457
                     /locus_tag="OO315_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892941.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            524..802
                     /locus_tag="OO315_004306"
                     /pseudo
     CDS             524..802
                     /locus_tag="OO315_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            804..>1480
                     /locus_tag="OO315_004307"
     CDS             804..>1480
                     /locus_tag="OO315_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5892942.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLP"
BASE COUNT          362 a          375 c          389 g          354 t
ORIGIN      
        1 gtgagaaatc cctcgccacc tctgatgtgg caggcatcct caacgcaccc gcacttaacc
       61 cgcttcggcg ggtttttgtt tttattttca acgcatttga agttctggat ggtgccggaa
      121 tagaatcaaa aatacttaag tagcgcgcag ggataaaagg gatggacccc gaacagggga
      181 gtgctactta tctggaagga ttctgatgat gaacatcgaa gaactgcgta aaattttttg
      241 tgaagatggc ctctatgctg tgtgcgttga aaatggaaat attgttagtc attaccgcat
      301 tgtgtgtttg caaaaaaatg gggctgcgtt aattaatttt gtggatgccc gagtgacgga
      361 cggatttatc ttgcgcgacg gtgagtttgt cacttcatta caggcattga aagagatcgg
      421 aataaaagct ggcttttctg ctttttcaga agaataaact catctacaat cttgcgcggg
      481 gctgaactcc cgctgagtaa caccgtgcca ccggagaaaa ccgatggcac gcaacgcaaa
      541 atattacaat catgataatt cgaccgttct tgcccacacg cacgagcggt attctcacgc
      601 atttaagtca gactggtacc agcatccctc atgcactgaa gaacaggccg aatggcttat
      661 tcagtgttac cgcaggcgcg gatgcgaggt taaaaaagcc cttagcctcg actaccgtca
      721 ctagataatc tccgtcaggc tcccttactc cgaacagcca ccgcgtccgt cccgcacatt
      781 ccagcaacgg atctggaggt aacgtgcggg tattacttcg acctgttctg gttccggaac
      841 tcgggctggt ggtccttaag cccggtcgtg aatccatgcc ggtattccac aatacccggg
      901 tactggtgga gccggaaccg aaaagcatgc gtaatctgcc gtccggggtc gttcctgccg
      961 ttcgccagcc gctagtggaa gacaaaacat tgctgccgtt tttcagtaac gcacgggtga
     1021 ttcgtgctgc tggtggtgct ggcgcattgt ctgactggct gttgcgccat attaaatcct
     1081 gccagtggcc acacggcgat tatcatcaca gcgaaaccgt cattcaccgt tatggtaccg
     1141 gcgcaatggt attgtgctgg cactgcgaca accagctgcg tgaccagaca tccgaatcac
     1201 tcgagcaact tgctcatcaa aacctgtcag catggatgat tgacgtcatc ggtcacgcaa
     1261 taagcggtac gcaggagcgt gaattatctc tggctgaatt atcctggtgg gcggtccgca
     1321 atcaggtggc ggacgcgcta ccggaagcgg tattacgtcg ttcgctgggg ttgcgtgcgg
     1381 aaaaaatccg ctcaatgtac cgtgaaagcg acatcgtgcc gggagagcag accgccacca
     1441 gcatactgaa gcagcgcaca aaaaatcttg cgccgctgcc
//
LOCUS       DAJTRD010000267         1475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTRD010000267 DAJTRD010000000
VERSION     DAJTRD010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.273"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..797)
                     /locus_tag="OO315_004308"
     CDS             complement(<1..797)
                     /locus_tag="OO315_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5892943.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLT"
     gene            complement(1396..>1475)
                     /locus_tag="OO315_004309"
     CDS             complement(1396..>1475)
                     /locus_tag="OO315_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="damage-inducible protein DinI"
                     /protein_id="HCR5892944.1"
                     /translation="KDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          394 a          235 c          353 g          493 t
ORIGIN      
        1 gttaatcttt gcagggattg cagagcatgg gatgacaatg ggttatcact aatatctatt
       61 gaacattcat tcctcagatt aagaatgctt tccgggatat gacttatctg atttatgtca
      121 aaataaccgt tccttaaatt ttccggcagg tatggaatat cggttaacat attgtcgatt
      181 gcccatatgg agtgaatgtt gtgaggtaga ggggggagtt cactaagaag attccctatt
      241 gcgctaatct cttgtaaaga aaatggtaaa ggtggtaaaa ctgccagccc attacatgat
      301 acatctaatg tttccagtag ttctggtaaa acaggaagag aacataattg attatttgag
      361 acatgaagct ccttcaaaga tatggggagt gtgggtagtg tgattagttg attgtgggac
      421 gcattcaata atttaagtcc ttgaggcaaa gcaggcagtt caataagtct gttatagctg
      481 acattaagtt gtgtaaggga cgcaggcaat ggggagatta agcttaaatt atttttactt
      541 atattaattg atttaattcc cggggggatt tcaggtaatg ttgtcaggcc taattcagac
      601 aagtccaggt tcgtctcttg gttttgtaga catgatacta gtcgctgaaa agcgatgtct
      661 cgttgttctt cttgtatgcg gttatttttc cattcagtcc aatgggttag ataattttca
      721 tatgcgcggc tagtgtcgat tgaataagtg gagaacgaat ttgaaattaa tctgtgattg
      781 ttatttgtcg ggagcataaa tatcagggtg tattctttat gttgagaggt gttggattct
      841 tttttatctg ctttgcaatg ttattggttc ccttcagcag tagacaggaa cttctggagg
      901 aggcggatat atagtttgtg taagcatatt aactccatat gttatatatt gaagaacttc
      961 tgctttacac tatcctaaac tggatcggtt taatgtaaaa aaacgatact ttttgggggg
     1021 gggcaatttt tccttaaggt atgaaaaaag gagctgaagc tatataatag ctattaatgc
     1081 cactggtatc agtgaatcat gtatataaaa aactgtgtta aggaggctgt ccattagcgg
     1141 ttttttattc ttctggaacc gcggcatctt tgcgtatctt tttgtcttct caccgtctgg
     1201 tcggtgtttt gctgagactg ctaatttcct gtctttgcta gtgctgtact ggtactgccc
     1261 aatcatgatt ggcgggggcg ggcggagttg agaccgcaga cacgtcgtat gcaagaacgt
     1321 gctgcggctg gctggtgaac ttccgatagt gcgagtattg aatgatttcc agccgttact
     1381 gattttacgt gctaattagt gaacaaacca ctcgtcagca gactcccagg tatctttcag
     1441 agtttcctga acaaaagttt tagctgaatc tttat
//
LOCUS       DAJTRD010000268         1423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTRD010000268 DAJTRD010000000
VERSION     DAJTRD010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.274"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(52..771)
                     /gene="yehT"
                     /locus_tag="OO315_004310"
     CDS             complement(52..771)
                     /gene="yehT"
                     /locus_tag="OO315_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5892945.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(768..>1423)
                     /gene="btsS"
                     /locus_tag="OO315_004311"
     CDS             complement(768..>1423)
                     /gene="btsS"
                     /locus_tag="OO315_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5892946.1"
                     /translation="EGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNAL
                     NTIKAVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQS
                     RLQVNIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEI
                     EDNAGLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          347 a          383 c          324 g          369 t
ORIGIN      
        1 atcatgaggc cttcaggtgt tgatgaggca aaaagccatt ttagcagtct tttacaggcc
       61 aatcgcctct tttaagcttt tcagataacg gcggctgacc ggcacggtta agccattacg
      121 caaaatcaac tcggcctggc cgttatcttc cagacgaatc tcctgtaaat gcgcgaggtt
      181 aaccagatac tgacgatggc agcgcagtag tggtgtacga ctttccaagg tacgtaatgt
      241 caattcggta aagccctctt tcccttcgtg gctggtaacg tagacaccgc tcatccgact
      301 gctgacaaat gccacatctt tcatttgcag caaataaatc cgactatgcc ccgtacaagg
      361 gataaatttc agcgcctgtt gattttccgg taacagcgaa acatcctgct tgctgcgctc
      421 ctgacgcaat cgcgccagcg ttttctccag tcgcgcttca tcaattggct tcagcagata
      481 atcaaaggca tgttcttcaa aggctttaat agcgtattcg tcaaacgcag tgagaaaaac
      541 aatatacggg cgatgttccg ggtcaagcat ccccaccatt tccagaccac tgatgcgcgg
      601 catctggata tcgagaaaca gcacatccgg gcgcagttta tgcaccgcgc cgatcccttc
      661 cacggcgttt gaacactctc caacgatttc aatatcgctc tgctcctgca aaaatatacg
      721 caggttctcc cgtgctaacg gttcatcatc gacaattaag actttaatca tgcctcgtcc
      781 ctccatggta gtcgtaacgt tattcgggtg taactatcag gctcacaggc gacgcttatt
      841 ccatagtcat cgccaaaccg ttcacgtaaa cgcttatcca ccagattcat ccccagccca
      901 ctggcattgg ttaccggttg atacaaaccg gcattgtctt cgatctccag catcaaatgt
      961 tgcccctcac gtcgggcgct gattgccact cgccctgtat ccagcagttg tgatgtccca
     1021 tgtttaatgg cgttttccac tatcggttgc agggtaaacg cgggcaattg ctgctgggat
     1081 aattcttgcg gaatagcaat gttgacctgc aaccgcgact ggaagcgcgc cttttcaatt
     1141 tgcagataag cgttcacatg ttcaatttcg tcggcgagag taacaaactc cgaaggccgc
     1201 tttaagtttt tgcggaaaaa agtggaaaga tactgcacca gctggctggc ctgttcgctg
     1261 tcgcggcgga tcaccgcttt aatggtgtta agcgcattaa acaaaaaatg aggattcacc
     1321 tgggcgtgaa gcagtttgat ttctgactgg gtgagcatcg ctttttgccg ctcatattgc
     1381 cctgcaagaa tctgcgccga aagcagttgc gcaatcccct cgc
//
LOCUS       DAJTRD010000269         1411 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTRD010000269 DAJTRD010000000
VERSION     DAJTRD010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1411
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.275"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..334)
                     /locus_tag="OO315_004312"
     CDS             complement(<1..334)
                     /locus_tag="OO315_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR5892947.1"
                     /translation="MLNVAIENQNGWNYSASAPHKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMAT
                     IPALSHPDV"
BASE COUNT          400 a          335 c          308 g          368 t
ORIGIN      
        1 ttacgtcagg gtgagaaagg gcagggatag tagccatgat ggcatcctcg agtgataagt
       61 taattaactc accaccgagg ttttccacga ccataagggt ggtgagacgt acaggggtgg
      121 aaataccggt cactcgagaa cccggccagt cttgcgactg ccctgcacat cccaccataa
      181 tttgaatgga gctgtgcatt acgcataaaa aaaccgcttc agcgcggcta tgcgctcgag
      241 taacgttcgg gtttccacgc ccggcccccg ttttatgagg tgcagatgca ctataattcc
      301 acccgttctg gttttcaata gctacattca acattttctc ttacctttca tcaccgaagt
      361 gaactttgtt gatgcggtgc ctggtgcctc caggtgacgt taaccagtta acaattaacg
      421 ccggatacag ggaaacccat aaccacccga tacgtgggaa ctaatcgctt tttaactgtt
      481 ccgcgtgcgc tcagccgcat tcaccgcatc acaaaattca ctttaaaaag ggcggacatc
      541 agccagcaat gaaactgatg ccgccaaagg taccaatcaa catggagtgt tgtggcgtgg
      601 ttgtcactta agcgtatggt caacctgaca acccggtgtc ctcaacgggg aaggaataac
      661 tccgccatac ttaccgccgc gtcatttcgc gttgtgtgcc tgcttttaac tacgtcaggc
      721 gaggtggttc ctgttattcc ccaacaacaa gaaatttgta taatctgaat accccaacaa
      781 taagaagagt gagcaagtga tgtattagcg atgtcatctc tcaaagagac tgcatcaatg
      841 ttaaaattat tcaatgaagc aaaaactcag gctgaggttg atcgcgctgt gtctgaaata
      901 aactctaagt tgtcaagcat tcagcttgaa tatttgtcat ctcattgatg agctgacaat
      961 ctctaaaagc aatgaggctg cgctcaaagc aaaactcacg gagatagaaa agtttaagtt
     1021 ccaaagtgaa gactgcgaac ctcgctaatt gattactgga acgattgtgt atgccagaca
     1081 gtgcaatact agctctggca agattaccat gtatttttgt ccagaatgtt ttgcgaagag
     1141 agcaatatct atattgcaac ctttccctat cgaggaaggc gataaatata cgaaaactca
     1201 atgcccctcc tgtaaaaatg agtatctcat ggactacaat ttcaataatg atttttgagt
     1261 cacggtattc agatttttaa agaacatgcc ggatactcac ccgtgtccgg cgcacgcact
     1321 ccacctcacc cgtggagaac tccttaatta ccaaccttgg cttcgttggt tagccattaa
     1381 cgctggtatg taatcattct ggcaatgctt a
//
LOCUS       DAJTRD010000270         1405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTRD010000270 DAJTRD010000000
VERSION     DAJTRD010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.276"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..168
                     /locus_tag="OO315_004313"
     CDS             <1..168
                     /locus_tag="OO315_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5892948.1"
                     /translation="SAIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQR
                     MKERRKEWGTL"
     gene            153..791
                     /locus_tag="OO315_004314"
     CDS             153..791
                     /locus_tag="OO315_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5892949.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1019..>1405
                     /locus_tag="OO315_004315"
     CDS             1019..>1405
                     /locus_tag="OO315_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892950.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          394 t
ORIGIN      
        1 tccgctattg ccagtacacc tcacccgtcc tggcgacgta tctgtaagac actcataaag
       61 aatgatttct ggtgccggac tctctccttc agtccgaaca aaccccggca ctatgaacgt
      121 tacctgcagc gtatgaagga aaggaggaag gaatggggga ctctgtgaca ccggagatac
      181 ttggcaacat gattcgtcag tatttcagtc aggtacgaac tgaaaaagag agcatacagg
      241 ctctgaatca tctgcgcaga gtgcttcatg aggtcagtcc ttttgcacag gagcctgtgg
      301 actgtgtgtt gtgggtgaaa gcggatgagg tggtggccaa cgattacaac cctaatgtca
      361 tggcactagg tgagaaaaaa ctactgaaac agtctctgga gaaagatggt ttcacacaac
      421 cggtggtggt gtcagaggag aagaatcatt atctggtggt ggatggtttt catcgtcagt
      481 tactggggcg ggaagcagat gcaggaaaac gcctgagggg ctggttgcct gtagcctgca
      541 ttaatccgga gagaaaggga caggccgcac gtattgccgc gaccattcga cataaccggg
      601 ccagggggaa acaccagatc actttaatgt ctgatattgt cagggatctt tcacgtctgg
      661 gctggacgaa tgaacgtatt ggtacagagc tgggaatgga ccaggatgaa gtgctgaggc
      721 tgaaacagat tagcgggctg acagagttgt ttcaggaaga ggatttcagc tcggcttgga
      781 cggttcgttg atgttgttaa atatgggcgg tatttctgaa agttgatact ttgggtagaa
      841 aaaatgatac cgaaaactat ggaggatgtg cttgtcaggt gactggacac cataggtctt
      901 aggctttgat aacgggataa gatgagcggc ttttctcata ccatcaggtg tgatgatggc
      961 gcagatttac aaaatctgat tgattctaaa gatgtataag taaaaatcaa gctttgatat
     1021 gaaaaaacag atatttatta ataataaacc tcctgtagtc ccttactcag ggacgcacgc
     1081 gaaaatattt aaatacattg aaatccctct gccgtttttc tactttatct atacatcagg
     1141 cgaaccattc cacatatctg tccaaaatac tgttatttat gtatcaaaat acaatggtat
     1201 atttattaat aaattagttc ctttttctct tttatttgac agggatatat ctgttcttca
     1261 aagaagagat atttgtgttg tgcgatttac gtcagaggaa ataagtgaac ataacgtttt
     1321 atttgatcat gacattgaac gattaaaaaa aatttccaaa gctcagctta tatctcccga
     1381 ttatgttttg attgattttt ctagt
//
LOCUS       DAJTRD010000271         1346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTRD010000271 DAJTRD010000000
VERSION     DAJTRD010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.277"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(3..485)
                     /locus_tag="OO315_004316"
     CDS             complement(3..485)
                     /locus_tag="OO315_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5892951.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="OO315_004317"
     CDS             complement(476..778)
                     /locus_tag="OO315_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5892952.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="OO315_004318"
     CDS             1050..1322
                     /locus_tag="OO315_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892953.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          396 a          338 c          287 g          325 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccgaccgct gaaacg
//
LOCUS       DAJTRD010000272         1336 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTRD010000272 DAJTRD010000000
VERSION     DAJTRD010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1336
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.278"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(207..362)
                     /gene="hokD"
                     /locus_tag="OO315_004319"
     CDS             complement(207..362)
                     /gene="hokD"
                     /locus_tag="OO315_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5892954.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(590..1255)
                     /locus_tag="OO315_004320"
     CDS             complement(590..1255)
                     /locus_tag="OO315_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5892955.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          331 a          342 c          314 g          349 t
ORIGIN      
        1 ccatccctct tctccctgcg cgctacttaa gtgagtcgat tctatctgcg aaggtgcgcg
       61 aatcaaattc accggaaata aacaacaaaa aacccgccga aacgctgaga gatcacagcg
      121 atgggcgggt taagtgcggg tgcgttgagg atgcctgaca catcagaggt gggcggggat
      181 gggatcagct ccccacccgg tcactcttac ttcctggatt cgtagtctac gaagacagcg
      241 acctccgtct ggccggttcg gattcgtacc tcacagaggt ctttcctcgt taccagtgcc
      301 gtcactatga cggttaaaca gatgacgatc agggcgatta acatcgcctt ttgctgcttc
      361 atagcctgct tctccttgcc tttcggcatg taagaggcta acctacatgt gcaaagcatg
      421 aaattggcct cagattaatg ttaagcgtct tgccggacgc gtaatgttaa ctggggcttt
      481 tctctatctg ccttttggtg ttcatgcctg aagcagatag cctcaagcac ccgcagtcat
      541 tctacttacc tcgccaatat gaaatcaatc agaaaggtgc cccataaaat cactccttct
      601 cttctttacc gtagtggagt tgaccaattt tgataagagg gcgtccctga gatttgcggt
      661 gtagattggt atcgcgcaga gaatacacac agccacaata ttcctgctga tagaattttt
      721 cgcgcttgct gatttcaatc atacgggacg agccgccctg cttgcgccag ttataatccc
      781 agtacaccat gcccggataa tgcgcgacgg ctcgccgccc acagtcgtta acctgctgca
      841 tatttttcca gcgtgaaatg cccagtgaac tgctgatcac actgaaacca ttttcagcgg
      901 cgtacagcgc tgtccgctca aaacgcatgt caaaacacat ggtgcaacgg atccctcgtt
      961 cgggctccca ttccattcct ttggctcgtt caaaccagtt gtctgtgtcg taatcagcat
     1021 caataaacgg cacgccgtgt tgttcagcaa agcgaatatt ctcatcctta cgaattaaat
     1081 actctttctg aggatgaatg ttcgggttgt agaaaaagat ggtgtagtcg atttccgagg
     1141 cctgaagcgc ctccatcacc tcaccggaac atggagcaca gcaagagtgc agtagtagtt
     1201 tgtttgcccc gtttgggagc tccaatttgg gccgtttgaa atcagcaact gtcataaata
     1261 tgtttattga ggtcataaaa atatcaaaga gtgtagcatt agaacggggc tatcggaaac
     1321 agatgtgtga ctgctc
//
LOCUS       DAJTRD010000273         1330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTRD010000273 DAJTRD010000000
VERSION     DAJTRD010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.279"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(41..100)
                     /locus_tag="OO315_004321"
     CDS             complement(41..100)
                     /locus_tag="OO315_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5892956.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            complement(339..1247)
                     /locus_tag="OO315_004322"
     CDS             complement(339..1247)
                     /locus_tag="OO315_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892957.1"
                     /translation="MTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRILAERQIQE
                     TEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEARANL
                     ALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAEMQA
                     AAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKSVEP
                     MKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPLIDSLLNE
                     IGVSGGSLAALTSPLISTTPVAENVK"
BASE COUNT          266 a          354 c          321 g          389 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agattaaggg tgaagtcaat gccgccagtg aaccgcctga aacgccaatt
      421 tcattgagca aggagtcaat cagcggagcc tgtgtgcggt aagagagagc ggctgataat
      481 gcttgttccg ctaggtttcc gccccccaca ttacccgtgt ttgcatcacc cgcagcgctg
      541 ccactattca gaccatcgac ctgaataatc ttgataccgt caatagattt catcggctca
      601 acggattttt ctattaccgc aggtagcgcc tgcaacaagg ccagtttgaa tttaaggctg
      661 gtttgttcat cagaaagtac gttgatagca tcgttcagcg cacgttgtgc ttctgcttcc
      721 gccagaccct ttttatgtgt agcttcggct aactcaacga tagccgccgc ctgcatctct
      781 gcggcttctt tttctgcttt cgcccgcacg gtcagttcaa ccgctttggt ttctgcatcc
      841 tgcgcggcag cgattagggc aacttgttta gcacgatcgg cttctgcggt ctggcgagtg
      901 gtttctacgt tttgttgcgc acttactgct tctgcgagtg caaggttagc acgcgcttct
      961 gcctgggact gttgttccga tttggcggca atagcgatcg atttcgtctg gttggcgatt
     1021 tcggtgacct gctgctgttc gatctcttta atacgaactt cacgttcagc ctcaaccttt
     1081 cttgagcgga cggcctgctc gcgctcgatc tccgtttcct gaatttgtcg ttcagccaga
     1141 attcgcgtct gctctgcttc acgacgacgt tcagcttcaa aagccgcaat acgtgcattc
     1201 tgttcggcag tacgggtttt aacctgctgc tcctgctcaa gcgtcataaa cgcttcttgt
     1261 tgttcggtaa tgactccaac ttactgatag tgttttatgt tcagataatg cccgatgacc
     1321 ttgtcatgca
//
LOCUS       DAJTRD010000274         1288 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTRD010000274 DAJTRD010000000
VERSION     DAJTRD010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1288
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.280"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            522..806
                     /locus_tag="OO315_004323"
     CDS             522..806
                     /locus_tag="OO315_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR5892958.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            806..1081
                     /locus_tag="OO315_004324"
     CDS             806..1081
                     /locus_tag="OO315_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5892959.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
BASE COUNT          339 a          322 c          325 g          302 t
ORIGIN      
        1 acagaataag tttacgcgta aatcgccctg aatccagaag agcattaagt atcacataaa
       61 caggttccat accagtcctg accgcatcag gacgccagta aataaaatct cgtagtccac
      121 cggcatcaga gacaagagta atcgtcgtta ccggtatttc gactggtttg tactggtgtg
      181 agcggacaaa actactgtac gtctctcata cacgctcgta agcggccctc acggcccgct
      241 aacgcggaga tacgccccga ctgcgggtaa acccttgtcg ggaccactcc gaccgcgcac
      301 agaggcattt tcatcgctga caggccgcat ggcctcgcag agccggaggg ggactggtga
      361 tattcggacg accgtaccgg cttgcgccgg gcttcggcgg ttttacacct gtgctacatg
      421 taaccaccgt atgccgcctt atatgcgctg actcgcatta tttgccttgt tccgtctgaa
      481 agtaatcact atgattaaat atgattaaca actaatcggg tatgcaaatg aaaaacaata
      541 ccgcacaagc aacaaaagta attaccgcgc atgtgccatt acctatggct gataaagtcg
      601 accagatggc cgccagactg gaacgctccc ggggctgggt tatcaaacag gcgctttctg
      661 catggcttgc ccaagaggag gagcgtaatc gcctgacgct ggaagccctg gacgatgtga
      721 catccggaca ggttatcgac catcaggctg tacagtcctg ggcggacagc ctcagtactg
      781 accatctgtt accggtgcca cgctgatgga actgaagtgg accagtaagg cactttctga
      841 tttggcgcgg ttatatgatt ttctggtgct ggccagtaaa cctgctgccg ccagaacggt
      901 gcagtccctg acacaggcgc cggtcattct gttaactcat ccacgtatgg gagaacagtt
      961 gtttcagttt gaacccaggg aggtcagacg gatttttact ggcgagtacg aaatccgtta
     1021 cgaacttact ggccagacta tttatgtatt gcgcctgtgg cacacacgag agaacaggta
     1081 gattgggtgt ggaggcaact ttaacatagc ctgcaggtat taattctgga gcattgttat
     1141 caggccagat attttgcatt ccattaaata atcttgtagg agataaaata aattacgatg
     1201 aaataacgaa aattaccgct cgtgactggc ggcaatatcg cgctccgggc tggcaaataa
     1261 cccatcagaa acgttattgc cagacatt
//
LOCUS       DAJTRD010000275         1280 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTRD010000275 DAJTRD010000000
VERSION     DAJTRD010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1280
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.281"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(74..1279)
                     /locus_tag="OO315_004325"
     CDS             complement(74..1279)
                     /locus_tag="OO315_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011906920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5892960.1"
                     /translation="MAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVASYL
                     KDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAANMPL
                     YQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYGLAK
                     GKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLTPIE
                     AARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLIEEQ
                     LIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWVPNF
                     GVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLPVAR
                     LEDGTLLNW"
BASE COUNT          341 a          346 c          287 g          306 t
ORIGIN      
        1 cgtccatttt tagctccttg agcggaatta atcgatttga tgactattca gaaaataatt
       61 aagcgaaccg ttattaccag ttcaacaatg tgccatcttc cagtcgtgcg acggggagat
      121 actccgggtc atacggatat ttcgccgcca gtttctcatc gaaagaaata cccagccccg
      181 gttcatcgcc tgggtgcata taaccttcct cgaagcgcca gctgtacggg aaaacttcaa
      241 gcatttgttc ggaatagccc atgtactcct gaacgccaaa atttggtacc cataggtcaa
      301 aatgcagcgc cgccgcatga caaacaggcg ataaatctga agggccatgc gaaccggtac
      361 gtacctgata aagcgatgca aaatcggcaa tccgccgcat cccggtaatg ccgcctgcat
      421 gggtgatcgt cgtgcgaata tagccaatca gctgctcttc gattaactgc ttgcagtccc
      481 agatactgtt aaacacttca ccgaccgcaa tcggcgtcac ggtatgctgg cgaatcagac
      541 gaaaacattc ctggttctcg gcaggcgtcg gatcttccat ccagaacagg cggtaatcct
      601 caatgctctt cccaaagcgc gccgcttcga tgggggtcag gcgatggtgc atgtcatgca
      661 acagatgttc gttaaaaccg aacttattac gcaccgcttc gaataacttc ggcgtaaaat
      721 cgagatactt ttctgaagac cacaactgct cttctggcca caggcctttg gttgccggtt
      781 catacgccag tcctttgccc ttcgccaggc cataagtggt ttgcataccc ggtacaccgc
      841 actgcacacg aatcgcctta aagcccatct gctggtgctt tgcatagtct tcaaggactt
      901 catcaatcgt acgcccggtg gtgtggcaat aaaccatcac gccttcacga gaagcaccac
      961 caagcagttg atacaatggc atatttgccg ctttagcttt gatatcccac aacgccatat
     1021 caatggcaga aatggctgac atcgtgacgg ggccacgtcg ccagtaagca cctttataaa
     1081 agaattgcca gatatcttcg atacggctgg catcgcggcc aattagttgt ggacaaagat
     1141 gatctttcag ataagaagcg accgacaatt cgcgaccatt taacgtggca tctcctaatc
     1201 cacaaagtcc gctttcagtg gttattttaa gcgtaacgaa attacgccct gggcaagtaa
     1261 caaaaacatc tgcagcaata
//
LOCUS       DAJTRD010000276         1275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTRD010000276 DAJTRD010000000
VERSION     DAJTRD010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.283"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            34..435
                     /locus_tag="OO315_004326"
     CDS             34..435
                     /locus_tag="OO315_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5892961.1"
                     /translation="MAKKDDNLKRLRELAASLGREPDISGSAADIAQRVAELEEELAN
                     MDDTDIRDKSAHPENALTGHENEVISAQPETVIQNMDDLVTVVALVTLHTDALHATRD
                     EPLAFVPPGMAFRVFAGVAAGMTERGLARMQ"
     gene            447..818
                     /locus_tag="OO315_004327"
     CDS             447..818
                     /locus_tag="OO315_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5892962.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            805..>1275
                     /locus_tag="OO315_004328"
     CDS             805..>1275
                     /locus_tag="OO315_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5892963.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          303 a          285 c          396 g          291 t
ORIGIN      
        1 gggctcccgt tttttattcg gacaggaaca gatatggcaa agaaagacga taacctgaaa
       61 cgtctgagag aactggcagc atcgctggga cgtgagccgg atatctcggg aagcgcagca
      121 gatattgcgc agcgtgtggc tgagctggag gaggaacttg ccaatatgga tgacactgac
      181 atccgggata agtctgccca cccggaaaat gcgctgaccg gacatgaaaa tgaggtgata
      241 tcagcgcagc cggagaccgt gattcagaat atggatgatc tggttacagt cgtggcactg
      301 gtgacgctgc ataccgatgc acttcatgcc acgcgggatg aacctctggc atttgtgccg
      361 ccgggaatgg cgttccgtgt ctttgccggt gtggcagccg gaatgacaga acgtggcctg
      421 gccagaatgc aataacggga ggcagcgtgg ctgatttcga taatctgttt gatgttgcac
      481 ttgatctcgc agacaaggcc attattcgca atatggggat tagagcggtc attacgtcag
      541 gccggctaaa agggatcatg atttccgggg tttttgatga tcctgaaaat atttctctgg
      601 tggccggcgg tgtgcgtatt gaagactctt taccatccct gtttgtgaaa acagcagata
      661 ttttacggct gtgtcgcaat gattcgctga tgattggtcg tgagtctttc tgtgtggatc
      721 gtatcacccc tgatgatggc ggatgtagtt atatccggtt gcggcgtgag gggctgccgg
      781 gaaacgtaag ggcaggacga tattatgaag gggcttgaga atgccatccg caatctgaac
      841 agccttgata cccgtatggt gccacaggcc agcgcatggg cgataaaccg tgtggcacag
      901 aaagcggtct cggttgccac ccggcaggtt gccgggaata ccgttgcggg cgataaccag
      961 gtgaaaggga tccccctgaa actggtacgt cagcgtgtcc gggtgtttaa agccagtccg
     1021 tcaggaaaaa tgacggccag gatccgcgtt aaccggggca atctgcccgc catcaaactg
     1081 aacacaacac ggcggcgtgc tggtgaagga ctgagagtgg gaaaatactt tttccggggg
     1141 gcatttgttc agcaactggc gaatggccgc tggcatgttc tgaggtgtct tcctgaagcg
     1201 cgttttgcaa cagggcatga ccatcagggc aggctaagaa aaaatcgtct tcctgtggag
     1261 gtagtgaaaa tcccg
//
LOCUS       DAJTRD010000277         1268 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTRD010000277 DAJTRD010000000
VERSION     DAJTRD010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.284"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..244)
                     /locus_tag="OO315_004329"
     CDS             complement(<1..244)
                     /locus_tag="OO315_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5892964.1"
                     /translation="MLTSEQHRCHNEKRNFSAEFKRESAQLVVDQKYTVADAASAMDV
                     GLSTMTRWVKQLRDERQGKTPKASPITPEQIEIRELR"
     gene            430..>1268
                     /locus_tag="OO315_004330"
     CDS             430..>1268
                     /locus_tag="OO315_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5892965.1"
                     /translation="MLNGGGILFKANHVPVLMYHHVSHCPGLVTLSPETFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLNEFNLKAHIFLIT
                     SFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFTSREEQCKHLRQDLLSGREYLKKMTGKCSKHLCWPEGYYNKDYIQIAEELG
                     FHYLYTTERRMNAPAKGTTRIGRISTKERENCVWLKRRLFYYTTPFFSSLLALHKGPR
                     LPD"
BASE COUNT          362 a          233 c          275 g          398 t
ORIGIN      
        1 tcctgagctc acggatttca atttgttccg gggtaatggg ggaggctttt ggtgtttttc
       61 cctgccgctc atcacgtaat tgtttcaccc atcgcgtcat tgtggaaagg ccgacatcca
      121 tagcgctggc tgcatctgcc acggtgtatt tctggtcaac gaccagttga gcggattcgc
      181 gtttaaactc tgcgctgaaa tttctttttt cattatggca cctgtgttgt tctgaggtga
      241 gcatatcacc tctgttcagg tagctaaatt tagtgtgcca cctcaaggtt cttagtgagc
      301 cgtttttaaa agatatgata atatattatt gtttataaat tgttgatgtt tgatttagta
      361 gtatataaag ttcaccacct ttattaggct ttccttttat ctctacttac tgggtgccag
      421 aaactttaaa ttttaaacgg agggggcatt ttgtttaaag caaatcatgt tccggtatta
      481 atgtatcatc atgtcagcca ttgcccagga ctggttacac tttctcccga gacattccgg
      541 aaacaaatga aatggttggc tgaaaacaac tggaaaacac tgtcatcaga tgaactggag
      601 tttttttatc ggggcggaaa acttccccgt aaaagcgtta tgttgacatt tgatgatgga
      661 taccttgaca actggtttca ggtatatcct ttattaaacg agtttaatct caaggcacat
      721 atttttttaa ttaccagttt tattggaaat ggtccggttc gtcactctcc tggaaaggaa
      781 tattctcatc gcgattgcga acatcaaata gcaaccggga atgctgataa tgttatgctt
      841 cgctggtctg aagtgaacga aatgttgcaa tctggtctgg tcgaatttca tgttcataca
      901 catactcata cacgttggga taaaaagttc acttcacgag aagaacagtg taaacatctt
      961 cggcaggact tattgagcgg gagagaatat ttaaaaaaaa tgacaggaaa atgcagtaaa
     1021 catttgtgtt ggccggaagg ttattataac aaggattata ttcagatcgc tgaagaactt
     1081 ggattccatt atttatatac aacagaaaga agaatgaatg ctcctgctaa aggaactaca
     1141 cggataggaa gaataagcac caaggaacgg gaaaattgcg tatggctgaa aagacgactt
     1201 ttttattata caacaccgtt tttttcatcg ttgctggccc ttcataaggg gccaagatta
     1261 ccggatga
//
LOCUS       DAJTRD010000278         1212 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTRD010000278 DAJTRD010000000
VERSION     DAJTRD010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1212
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.285"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..261)
                     /locus_tag="OO315_004331"
     CDS             complement(<1..261)
                     /locus_tag="OO315_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5892966.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAVNEALRRMKKAGIEKPELEAFLREMING"
     gene            complement(258..548)
                     /locus_tag="OO315_004332"
     CDS             complement(258..548)
                     /locus_tag="OO315_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5892967.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQVAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(548..1147)
                     /locus_tag="OO315_004333"
     CDS             complement(548..1147)
                     /locus_tag="OO315_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5892968.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSDAAEQ
                     YLNTVATCRTRDTALLKSFDAFREWVTIQAGFYTEHIYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
BASE COUNT          279 a          325 c          274 g          334 t
ORIGIN      
        1 gccattgatc atctctcgca aaaacgcttc cagctcaggt ttctctattc ccgctttttt
       61 catcctgcgc agggcttcat tgacggctgt tttcgtcaat tttttggatg ccagcaactg
      121 gttaaacata tttcccgtct taccgccgcc aatatacgac cagcgccccc acatgcgcag
      181 ttttccctga atccagacac tttccagcgt ggtgagacga aggtgttccc cgcttttgcc
      241 tgtatttgtt gggtaaatca taaataacct tcctttctcc agatttcttg tgtgcgaaaa
      301 acaccttctg catgcatcag gcgtaattct tctttggtgt aatcgctggt ttttacccgc
      361 ccgtcgatta aatcgtggca tgagctacag gcaatcgcta cctgcatatc gtgtggtttt
      421 gtcgctgttc cgcacgtccc cgccagcctg taatgcgcca gcacagaagt ttcgggattg
      481 tgattgcagt agccaggaat tctgacggtg cacatctgcc cccgcgccgc tttacgtaaa
      541 tccaccatta cgcaaactcc agtaactgcg cggccacatt ttcgacttcc tccggagagg
      601 aaaatttacg gaacaggatc cagttccaca gcacattcag tacagattta taaacctgct
      661 gaaactcggt ttcgtccatg ttcgcaaatg cgatagattt tgccctgcgc ccacgactac
      721 catcaggata aatatgctcg gtgtaaaatc cggcctgaat ggttacccac tcgcggaaag
      781 cgtcaaacga ctttagcaat gccgtatccc gggttctgca tgtcgcaact gtattaaggt
      841 attgctctgc ggcatcactc agggctggag tgtgttcccg acctactgat tcgcacaggt
      901 aatcaacgaa gcctgatacc agttttcgtt cgcgaggcgt gatcgcccca ccgaccggag
      961 tccagtaatc aaatcccagt tgcaggagtt tgaaaaaacg cttgtggaat gcgtagttac
     1021 gcacacgctt aaagtctgcg tgtatccact cacctatttt gatttgatgc aaaaaatcgc
     1081 aactctccgg cgtcgccggg agaagtaatc cggaagaggt ttgtttgacc agttgtatat
     1141 gcgccatcgt agttctccgc tggcgcagta gaatgggtgt tcagcccgtt atgtagtata
     1201 ccagaattaa tg
//
LOCUS       DAJTRD010000279         1144 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTRD010000279 DAJTRD010000000
VERSION     DAJTRD010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.286"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..1006)
                     /gene="btsS"
                     /locus_tag="OO315_004334"
     CDS             complement(<1..1006)
                     /gene="btsS"
                     /locus_tag="OO315_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5892969.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     L"
BASE COUNT          322 a          308 c          258 g          256 t
ORIGIN      
        1 ataaccggtt tttggcttca tacaatttga tggtgcccat cacgcgctga ttttcgccac
       61 gcaacggaat taccagcgtc gaccccagtt tgcattgcgg atgcaaagag caacgataag
      121 gtacttcgtt gccatcagcg tagaccactt caccggtttc aatcgctttt aaggtgtaag
      181 tcgaagaaat cggtttgccg ggtaaatggt ggtcgtcacc aattccggta aaggccagca
      241 atttctctcg atcggtaatc gcgactgcac caatatccag ctcctgatac agtacctgag
      301 ccactttcat gctgttcact tcgttaaacc cctgtcgcaa aatgccttcc gtcgaggctg
      361 ccactttcag cgcagtggca gaaaaagccg aagtgtattt ttcaaacatc gcgcgtttat
      421 cgagcaatat acgcataaac agcgccgcgc cgacggtatt ggtgaccatc attggcgcag
      481 caatattact caccagacgc accgcatctt cataaggtcg ggcgatcgca aggatgatca
      541 gcatttgcac catttcagcg acgaacgtga cggcaccggc ggtaatgggg ttaaagactt
      601 tatcagtgcg cccgcgacgg atcaggatgc tgtgtaccag gccgccgagc aatccttcaa
      661 cgatggttga gatcatgcaa cttagcgcgg tcatgccccc catcgaatat cgatgtaagc
      721 cgccggtcag accaaccagc ccaccgacga ccggaccgcc gagtaagccg cccattaccg
      781 cgcctatcgc acgggtattg gcaatagaat cgtcaatgtg caacccaaac caggtgccca
      841 tgatgcagaa gatggaaaag acgatgtagc agagaaattt atgcggcaga cgaaccgtga
      901 cctgcattaa cggtatgaat aatggcgttt tactcattaa ccacgcaatg actaaaaaaa
      961 cgcacatctg ctgaagcagc agcaacacca gattaaaatc gtacataccc gcaaaccaca
     1021 cttcccttta aaacgcgtaa catacattgc ctgcgtttaa ctttctttga actcttgcag
     1081 aaaaatgaga attcgtgagt acgatcactc aaaatcgcct ggcaaaaata aaatcaccct
     1141 atag
//
LOCUS       DAJTRD010000280         1139 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTRD010000280 DAJTRD010000000
VERSION     DAJTRD010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1139
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.287"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..>1139)
                     /gene="ospC4"
                     /locus_tag="OO315_004335"
     CDS             complement(<1..>1139)
                     /gene="ospC4"
                     /locus_tag="OO315_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5892970.1"
                     /translation="CANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSLEAPSGARSS
                     FRSLEHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNI
                     KSFDKIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLPLNKTHHTVDFGAN
                     AYIIDHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTD
                     VPIFNTKDMRLGIGLHLIDFIRKSKDQGFREFCYNKNIDPVSLDRIINFVFQLEYHIP
                     RMLSTDNFKKIKLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALY
                     LLSKFNFTKQDVAEMEKMKNNRYCNLYDVEYLLSKDGANYKVLEY"
BASE COUNT          367 a          184 c          181 g          407 t
ORIGIN      
        1 aatattctag cactttatag ttggcaccat ctttactaag taagtattca acatcataca
       61 gattacaata tctattgttt ttcattttct ccatctctgc gacatcttgt tttgtaaaat
      121 taaatttaga tagtaaataa agtgctatat cagctttttt attgcctaaa gaataagcaa
      181 gggcctgatg agccattttt ttatcagtca ctttattgtt aatctcttca taattagatg
      241 ctttaatcgc atcctccaaa gatatgtctc tgagttttat ttttttgaag ttatctgtac
      301 ttagcattct tggtatatga tactcaagct gaaacacaaa gtttataatt ctatccagac
      361 taacaggatc aatatttttg ttataacaaa actctctgaa tccttgatct ttactctttc
      421 ttataaaatc aataagatgc aacccaattc caagcctcat atctttagtg ttaaatattg
      481 gaacatcagt tttcccctga ttaccatgca catacctgct aacttcatcc acaacctctt
      541 taaagttatc taagaaaaat gattggtgct catggtaaaa aacaggtgga atagcattat
      601 caaagtgatc ggttaatgtc atatatccat atggagagtc atgatcaatg atatacgcat
      661 tggcgccaaa atctacagta tgatgtgttt tatttagtgg gagtttttct tgatgattgg
      721 aaatttcgac cccaaaaaaa acaaaatcgt ggttagagat ttctttaata tcctcagagt
      781 atgtattctt atgtgtttgt atattttcag actgaatttt atcaaaggat ttaatattaa
      841 gaatattatt tgacagatta gagttacttt gatgtctgaa gttaaaatta gaattaataa
      901 taatagaaag taagtgacgt tcgtctgaag aaagaggatg aactttttgt attatctcag
      961 agatatagtg cctggacacc ttatcgagat gctcaagtga ccgaaatgat gaccgtgctc
     1021 cagatggcgc ttcaagagaa agatttagtg atttgaaact ggtcccttga gaaaacattt
     1081 tgctataact ttgcgctgct atagacttcc ttaaaaagtt tttcacagta ttagcacag
//
LOCUS       DAJTRD010000281         1100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTRD010000281 DAJTRD010000000
VERSION     DAJTRD010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.288"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            55..>1100
                     /locus_tag="OO315_004336"
     CDS             55..>1100
                     /locus_tag="OO315_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5892971.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          344 a          255 c          178 g          323 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt
//
LOCUS       DAJTRD010000282         1091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTRD010000282 DAJTRD010000000
VERSION     DAJTRD010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.289"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO315_004337"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO315_004337"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5892972.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(986..1062)
                     /locus_tag="OO315_004338"
     tRNA            complement(986..1062)
                     /locus_tag="OO315_004338"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1026..1028),aa:Asp,seq:gtc)
BASE COUNT          285 a          255 c          248 g          303 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ataagtggcg gaacggacgg gactcgaacc cgcgaccccc
     1021 tgcgtgacag gcaggtattc taaccgactg aactaccgct ccaccgaatt cttttacaac
     1081 caccggtttt a
//
LOCUS       DAJTRD010000283         1070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTRD010000283 DAJTRD010000000
VERSION     DAJTRD010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.290"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            20..562
                     /locus_tag="OO315_004339"
     CDS             20..562
                     /locus_tag="OO315_004339"
                     /inference="COORDINATES: protein motif:HMM:NF024120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892973.1"
                     /translation="MIGVKQPLPKAVLRTQHDKNKEAISILDFGVIDDGVTDNYQAIQ
                     NAIDAVASLPSGGELFIPASNQAVGYIVGSTLLIPGGVNIRGVGKASQLRAKSGLTGS
                     VLRLSYDSDTIGRYLRNIRVTGNNTCNGIDTNITAEDSVIRQVYGWVFDNVMVNEVET
                     ALFNARALALKIYSMSGWNL"
     gene            789..>1070
                     /locus_tag="OO315_004340"
     CDS             789..>1070
                     /locus_tag="OO315_004340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endorhamnosidase"
                     /protein_id="HCR5892974.1"
                     /translation="MEQLDLDYCGSTGVVIENVNGGFSFSNSWIAADADGTEQFTGIY
                     FRTPASTQSHKIVSGVHINTANKNTAANNQSIAIEQSAIFVFVSGCTLTG"
BASE COUNT          315 a          202 c          257 g          296 t
ORIGIN      
        1 taacaccaaa ggcgatgcca tgattggcgt caagcagcct ttaccaaaag cagttttaag
       61 aactcagcat gacaaaaata aagaagcaat aagtatcctg gattttggtg ttattgatga
      121 tggtgtgaca gataattacc aggcaataca aaatgctata gatgccgttg cttcactacc
      181 ctccggcggg gagctgttta tccctgcgag caaccaagcg gtggggtata ttgttggatc
      241 cactttgctt attcctggcg gtgttaacat cagaggagtt ggtaaggcat cgcaactccg
      301 agcaaaaagc ggacttacag gatctgtgtt aaggctgtct tatgattcag acactatcgg
      361 ccgttatctg agaaatatac gagtaactgg taataacacc tgcaatggta ttgacacaaa
      421 cattacagca gaagactctg tcatcagaca ggtttatggc tgggtatttg ataatgtaat
      481 ggtgaatgaa gttgaaaccg cattatttaa tgcaagggct ctggcactca aaatttatag
      541 catgtcaggc tggaacctgt agagtcggtc ttcacttttt aggccagtgc gtaagtgtta
      601 gtgtcagctc ctgccatttc agcagaggaa attattctgc tgatgaaagc tttggcatca
      661 ggattcagcc tcaaacgtat gcgtggtcgt cagaggcagt taggtcagaa gcaataattt
      721 tagacagtga gaccatgtgc attggtttta aaaatgccgt ctatgttcat gattgccttg
      781 atttgcatat ggaacaactg gatttagatt attgcggctc aacaggcgtg gttatagaga
      841 atgtaaacgg aggattttct ttctcaaact catggatagc agcagatgcc gatggcactg
      901 aacaatttac ggggatatat tttagaacgc cggcctcaac gcagtcacat aaaattgtca
      961 gtggtgttca tatcaacact gcaaacaaaa acacggctgc aaacaatcaa agtatagcga
     1021 tagaacagtc ggcgatcttc gtctttgtaa gtggttgtac gttaactggt
//
LOCUS       DAJTRD010000284         1064 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTRD010000284 DAJTRD010000000
VERSION     DAJTRD010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.291"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            76..594
                     /locus_tag="OO315_004341"
                     /pseudo
     CDS             76..594
                     /locus_tag="OO315_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          326 a          237 c          223 g          278 t
ORIGIN      
        1 cgaatctgct gaatcagaac tttacgccca cagcacctaa tcaggtctgg gtcgcagatt
       61 cagttgtcca ggccttccga aatcaaccga ctgaaggcgc tggtcgcgaa actgcagcgt
      121 atgcagttcg gtaaaagctc agaaaaactt cgcgcaaaaa ccgaacggtg gatacaggaa
      181 gcacaggagc gaatcagcgc acttcaggag gaaatggcgg aaacactggg tgagcaatat
      241 gacccggtac tgccatcttc cctgcgccag tcttcagccc gtaaaccgtt accagcctca
      301 cttccccgtg ccccccgggt catccggccg gaagaggaat gctgtccggc ctgtggtggt
      361 gaactcagtc cactgggatg tgatgtgtca gagcagctgg agcttatcag cagcgccttt
      421 aaggttatcg aaaaacaacg ccctaaactg gcctgtcgcc ggtgcgacca tatcgtgcag
      481 gcaccggtgc cttcaaaacc cattgcacgc agttatgccg gagcggggct tctggcccat
      541 gttgttaccg gaaagtatgc agaccatctg ccattatacc gccaaagtga tctactattc
      601 cacacagcta tttagctctc tttttgttga gctcccatga ttctttcatt tattgttact
      661 aattatttta tgttatcatg atgttagaac acgctctgct ctgtgtctct attttatcta
      721 tcatattctg aggattaact acataacaag gcgcaagata tatatacaaa aacaaataat
      781 accgttgcaa taattccttt tagtaaagga ggtgtgatta atttcagggg atttcagagt
      841 aaaaattgta caacatatta aaatatgttc atttcgttat aatacacaca aggaaatatt
      901 aagttagata atatattcta aataaccata gataaaaacg cacataatgg ggaatatttt
      961 atgaaaatac ctgaagcagt aaatcatatt aatgttcaga ataatattga tcttgttgat
     1021 ggaaaaacga atccgaataa agcaacaaaa gcattacaaa aaat
//
LOCUS       DAJTRD010000285         1020 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTRD010000285 DAJTRD010000000
VERSION     DAJTRD010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.292"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(50..190)
                     /locus_tag="OO315_004342"
     CDS             complement(50..190)
                     /locus_tag="OO315_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892975.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            329..1000
                     /gene="queE"
                     /locus_tag="OO315_004343"
     CDS             329..1000
                     /gene="queE"
                     /locus_tag="OO315_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5892976.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYEVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          296 a          205 c          237 g          282 t
ORIGIN      
        1 acatacgaaa gtgaacatta gtatgaaata gcgcattata aatttctcat taaatctgga
       61 taaataaatt gacggcggca tcggtaattt tagattcgtt atcattaaaa actaatacag
      121 aaaaaataaa tataattttg gtgaatgttt tgacatgatt aaatccattt tctttatttt
      181 cttctgacat aatacgttac tccctttaat tattaacgat atgtttcgta tttttcttta
      241 aatctgcatg taacagaatg ttaataagac atgattgttg cggcaggatc tgcaataatt
      301 aacaattatc cacttcacag agaatgctat gcagtacccg attaacgaga tgttccagac
      361 cctgcaaggt gagggttact ttaccggcgt tcccgccatt tttattcgtt tacagggatg
      421 cccggttggc tgtgcctggt gcgacaccaa acacacctgg gaaaagcttg aggatcggga
      481 agtctccctt ttcagcattc tggcgaagac caaagagagt gataagtggg gggcggcgag
      541 cagtgaagac ttgctggctg tcattggtcg tcagggatat accgcgcggc atgtggtgat
      601 tacgggtggt gagccttgca ttcatgattt gctgccactg actgacctgc tcgaaaagaa
      661 cggttttagc tgccagatcg aaaccagtgg aactcatgaa gtacgctgca caccgaatac
      721 ctgggttacc gtatcgccaa agctgaacat gcgcggcggc tatgaagtgt tgtcacaggc
      781 actggagcga gccaacgaaa tcaagcatcc agtggggcgc gtacgcgata ttgaagcact
      841 ggatgaacta ctggcaacgc tgaccgatga taaaccgcga gtcattgcac tgcagccgat
      901 tagccaaaag gatgatgcca cacgattgtg cattgaaacc tgtattgcgc gtaattggcg
      961 tttgtcgatg caaacacata aatatttaaa tattgcctga ttacacattt ataagcgtta
//
LOCUS       DAJTRD010000286         1012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTRD010000286 DAJTRD010000000
VERSION     DAJTRD010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.293"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..675)
                     /gene="ospC2"
                     /locus_tag="OO315_004344"
     CDS             complement(<1..675)
                     /gene="ospC2"
                     /locus_tag="OO315_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5892977.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYG"
BASE COUNT          329 a          149 c          174 g          360 t
ORIGIN      
        1 tccatatgga gagtcatgat caatgatata cgcattagca ccaaaatcca cagtatgatg
       61 tgttttattt agtgggagtg tttcttgatg attggaaatt tcaaccccaa aaaaaacaaa
      121 atcgtggtta gctatttctt caatatcctc agagaatgtg tttttatatg tttgtatatt
      181 ctcagactta attttatcaa aggacttaat attaagagta ttatttgaca ggttagcgtt
      241 actttgatgt ctgaagttaa aatcagaatt aataataata gagagcaagt gacgttcatc
      301 cgaagaaaga ggatgagttt tttgtattat ttcagaaaga tagtgtctgg acaccttatc
      361 gaggtgctca agtgaccgaa atgatgaccg tgctccagat ggcgcttcaa tagaaagatt
      421 taatgatttg aaactggtcc cttgagagaa cattttacta taactttgcg ctgctataga
      481 cttccttaaa aagcttttca cagtatcagc acagtgatct aaatgttttt cacttatgcc
      541 agaagaggat gagtttgtta cgcatgatat gtttttttgt aatgcttttg tatctttgtt
      601 cggatttatt tttccatcaa caagatcaat attgttctga acattaatat gatttactgc
      661 ttcaggtatt ttcataaaac atcccccatt atgtgcgttt ttatctgcag ttatttagaa
      721 taatattatc taacttaata ttttcttgta tgtattataa cgaacatatt ttaatatgtt
      781 gtacgatttt cactctgaaa tcccctgagt ttaatcacac ttcctttact gaaaggaatt
      841 attgcaacag tattatttgt ttttggatat atatcttgcg ccttgttatg tagttaatgc
      901 tcagaatatg atagataaaa tagagacgca aaacagggca tgctcataac atgatgataa
      961 cataaaaaat tagtaacaat aaatgaaaga atcatgggcg ctcaacaaaa ag
//
LOCUS       DAJTRD010000287          990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTRD010000287 DAJTRD010000000
VERSION     DAJTRD010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.294"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            82..>990
                     /locus_tag="OO315_004345"
     CDS             82..>990
                     /locus_tag="OO315_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
                     /protein_id="HCR5892978.1"
                     /translation="MPVHKVCQNFFRDALAPFHQYRQNALMDATMALINGASLTQTSI
                     GRFLPGNAQVKNKIKRIDRLMGNEALHRDIPMIFRNITSMLTRQLSLCVIAVDWSGYP
                     SQEHHVLRASLLCDGRSIPLLSKVVPSEKQNNPLIQHDFLDSLAQSLPPDARVIIVTD
                     AGFQSAWFHHITSLGWDFIGRIRNNVQYCLDNAPERWLKVSDSPECKTPEYMGAGRLV
                     KERKKSIRGHFYTYKKSAKGRKKKRSKGQSGLNKTDKEQSKSAKEAWLIFSSTNDFRA
                     REIIKLYSRRMQIEQNFRDEKNGRFGF"
BASE COUNT          301 a          206 c          228 g          255 t
ORIGIN      
        1 tgaggaatcc ccagaaaacg agacaaacat agcattatgt gatctactga tttgtgccaa
       61 caattcaatg agatcactgc tatgcctgtt cataaagtat gtcagaattt cttccgggat
      121 gccttagcac catttcatca gtaccgacaa aatgccctga tggatgccac aatggcgtta
      181 atcaacggag catctctgac gcagaccagt atcggacgtt ttttgccggg taatgcacaa
      241 gttaagaata agatcaaacg tattgatcgc ctcatgggta atgaggcgct ccatcgcgat
      301 attccaatga ttttcaggaa tatcacttca atgctgacac ggcagttatc tttatgtgta
      361 atagccgtag actggagtgg ctatccctcc caggagcatc atgttcttcg tgccagcctt
      421 ctgtgtgacg ggcgttccat tccgttattg agtaaggttg ttccttctga aaaacaaaat
      481 aatccattga tacaacatga tttccttgat tcccttgctc aatccctgcc ccccgatgcc
      541 agggttatta ttgtcacgga tgctggattt cagagcgcat ggtttcacca tattacttct
      601 ctgggctggg attttattgg tcggatcaga aataacgttc agtattgcct cgataacgcg
      661 cctgaaagat ggttaaaagt gtcggatagc cctgaatgta aaacaccgga atatatgggg
      721 gctggaaggc tggttaaaga aaggaaaaaa agcatccggg ggcattttta cacctataag
      781 aaatcggcaa aagggcgaaa aaagaaacgt tcgaaaggtc agagtggttt aaacaaaaca
      841 gacaaagaac agagcaaatc agcaaaagaa gcctggttga tcttcagcag tacaaatgac
      901 ttcagagcgc gagaaatcat caagttatac agtcggcgaa tgcagataga gcaaaacttt
      961 cgtgatgaaa agaacgggcg cttcggattt
//
LOCUS       DAJTRD010000288          952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTRD010000288 DAJTRD010000000
VERSION     DAJTRD010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.295"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            51..473
                     /locus_tag="OO315_004346"
     CDS             51..473
                     /locus_tag="OO315_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5892979.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            531..887
                     /locus_tag="OO315_004347"
     CDS             531..887
                     /locus_tag="OO315_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892980.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          278 a          192 c          256 g          226 t
ORIGIN      
        1 cgtccgaatg ttagccattc gagggttgtt aagtagtttc aggaggattt atggcgaaac
       61 cttttactcc cgaacagcgg gaagaactga agacgcgaat tgtggaactc gtgcatcagg
      121 acggtcgggt cacgattcgg cagttgtccg atgaaacagg tatcagtcgt gcgtctgtcg
      181 gtcgcttatg catagaactg gtcgcaagtg gtgatgtata taattctggc tacggcttat
      241 tcccgtctga acaggctcgc aaggactggc aaagcgcccg caaaaaactc tcgagagtaa
      301 aggtgaggaa accggttgtt gttgatccgg accttatctg gtcattacct gacggagaaa
      361 tacgccgcta cgacaggcgc ctgaatataa tctgtcgcga gtgccggaag agcgaagcta
      421 tgcagcgtgt actggctttc tatcagggta attttcagga ggcgatactg tgaatgaaat
      481 tagctatcag gcttcaatta ccgctggcat tcgcatcaaa ggagaggagc atggaaataa
      541 aaccagaaga tgagttaagc aatatcgttt tatttccgat aaaagaggat gaccctcgta
      601 atcaggttaa ttttctttat aagccatcgg aaagaccata ttgccatcac gcctctgtac
      661 gggttgacga aaaagagcgt caggtccgct gtaaaatctg cggtgcggtt gtggagccgt
      721 ttgactggat gctctctgtg gcaaaaagag aaaccagact ggcagatgat gtaaggctat
      781 tgcgccagga ggaacaggaa aggcggagaa atatagaaaa gctgatacag attgagcgta
      841 acgcgaaagc gcggatacgc agggcgacaa aatccagaac tgaataatta aatttagcac
      901 tgttaaaaat ttaatcctta accggaggga tttctgcacc ctcaaatcat ca
//
LOCUS       DAJTRD010000289          931 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTRD010000289 DAJTRD010000000
VERSION     DAJTRD010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.296"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..269
                     /locus_tag="OO315_004348"
     CDS             <1..269
                     /locus_tag="OO315_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5892981.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..616
                     /gene="tnpB"
                     /locus_tag="OO315_004349"
     CDS             266..616
                     /gene="tnpB"
                     /locus_tag="OO315_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5892982.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFTKRLAGDPGRESAPDASSVIHATGGDRVATSQTDR
                     TAWHPDITRDKTRE"
     gene            613..>931
                     /locus_tag="OO315_004350"
     CDS             613..>931
                     /locus_tag="OO315_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5892983.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          253 a          238 c          258 g          182 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtggg ccgatcgtga cgggttatgc ctgttcacca aacgtctggc
      481 cggtgacccg ggaagggaaa gtgcacctga cgccagctca gttatccatg ctactggagg
      541 ggatcgcgtg gcaacatccc aaacggacag aacggcctgg catccggata taacccgtga
      601 taaaacaagg gaatgaacaa tgaactcccc gatgatattg agctgcttaa agccatgttg
      661 cgtaagcaac agagtcggct tcgacagtat gcctgtcagg tcgcgggcta tgagcaggaa
      721 attgaacggc tgaaagcgca actcgacagg ttgcgtcgta tgttgttcgg ccagagttca
      781 gagaaaaagc gtcataagct tgaaaatcag atccgacagg cagaaaaacg actgtcggaa
      841 ctggaaaacc ggctgaacac agccagaaat cttctggaag atgcatcgtc agtcacagat
      901 tcacctgaca ccagtccccc gtcagaaaac c
//
LOCUS       DAJTRD010000290          914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTRD010000290 DAJTRD010000000
VERSION     DAJTRD010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.297"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(105..680)
                     /locus_tag="OO315_004351"
                     /pseudo
     CDS             complement(105..680)
                     /locus_tag="OO315_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            684..914
                     /locus_tag="OO315_004352"
                     /pseudo
     CDS             684..914
                     /locus_tag="OO315_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          209 a          255 c          215 g          235 t
ORIGIN      
        1 ggcacggctc agctccacgc cctgacgacg gtatatttct gactggcggt ataactgtca
       61 acgacggatg aaaagtggct cttccgcaac ctgggtggag attttcatgc cagtggactc
      121 attaccctct gcctgagtag ttcaaatcct gctcgaccat acatctggcg tttcagcatc
      181 ttcttcaggc gatttacatg accttcgaca acaccattac tccagcgact actgattgcc
      241 tcacatatag ctgcagcatc agcttccatc cccgccgcca cgcgccgaaa ttctgctgaa
      301 ccgctttcgt ggactcgagt gaaccaactg cttagctgtg atttattttg ggttttcaga
      361 atacggtaga actcgagtgc cagttgctgc gctattttca gctcaggttc tttttcacac
      421 atcagactaa taaatcggga agcataattt tcttcatccc tggttattct ccagggcatc
      481 aaccagcggc ttacccggga gactgaagga agtctgacgg cagtagtaac cggtgggttc
      541 cagcctttac gccatttggc aaccgcatcc ctgacgatgg tttcactgcc tgtaaatccc
      601 cgggccacca tttcccgcca tatccggctg gcgttataat taccactttc tcgttgctct
      661 tttaaccact ccctccatgg tgtcagcgaa aaacgaaacc gctgttgaaa tccctggaaa
      721 gctggttgcg gaaaagatga agacccagtc gcgacactca gagttggcga aggcgttcgc
      781 gtacgcactt aaccagtggc cggcactgac gtactatgcg aacgatggct gggtggaaat
      841 cgacaacaac atcgctgaaa atgccttgcg ggcggtcagt ctgggtcgta aaaacttcct
      901 gttcttcggc tctg
//
LOCUS       DAJTRD010000291          902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTRD010000291 DAJTRD010000000
VERSION     DAJTRD010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.298"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..902
                     /locus_tag="OO315_004353"
                     /pseudo
     CDS             1..902
                     /locus_tag="OO315_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
BASE COUNT          283 a          177 c          209 g          233 t
ORIGIN      
        1 agttcgattt cttgaaacgc ggcttatgta tctaaaaaag ctgaaagcct tagctcatcc
       61 cacgaaaaag tgaaagtact caacgagcta aggcagtttt atcctcttga tgagcttctc
      121 agggctgcgg agataccgcg cagtacgttt tattaccatc taaaggctct cagcaagcct
      181 gacaagtatg cggacgttaa aaagcgtatt ggtgagattt atcacgagaa tagaggccga
      241 tacggatacc gtagggtaac gctgtctctt catcgagaag ggaaacagat taaccataaa
      301 gctgttcagc gcctgatggg aaccctctca cttaaagcag cgattaaggt caagcgatac
      361 cgctcttaca gaggagaggt agggcaaacc gcccctaatg ttctccaaag agatttcaag
      421 gctacgcggc caaacgagaa gtgggttacc gatgttactg aatttgcagt caatgggcgc
      481 aagctgtatt tgtctccagt aatagatctc ttcaacaacg aagttatttc ttacagcctt
      541 tcggaaagac cagtgatgaa catggttgag aatatgctcg atcaggcatt caaaaagctt
      601 aatcctcacg agcatcctgt tctgcactct gaccagggat ggcagtatcg tatgagaaga
      661 tatcaaaata tccttaaaga acatggtatt aaacaaagca tgtccagaaa aggcaattgt
      721 ctggataatg ctgtggtgga gtgtttcttt ggaaccttaa agtcggagtg tttttatctt
      781 gatgagttca gtaatataag cgaactgaag gatgctgtta cggaatatat tgaatactat
      841 aacagcagaa gaattagcct gaaattaaaa ggtctgactc caattgaata tcggaatcag
      901 ac
//
LOCUS       DAJTRD010000292          901 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTRD010000292 DAJTRD010000000
VERSION     DAJTRD010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.299"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            18..901
                     /locus_tag="OO315_004354"
                     /pseudo
     CDS             18..901
                     /locus_tag="OO315_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
BASE COUNT          249 a          225 c          249 g          178 t
ORIGIN      
        1 acaacacagg tgctccaatg aaaaaaagaa attttagcgc agagtttaaa cgcgaatccg
       61 ctcaactggt tgttgaccag aaatacacgg tggcagatgc cgccaaagct atggatgttg
      121 gcctttccac aatgacaaga tgggtcaaac aactgcgtga tgagcgtcag ggcaaaacac
      181 caaaagcctc ccccattacc ccggaacaaa ttgaaatccg tgagctcagg aaaaagctac
      241 aacgcattga aatggagaat gaaatattaa aaaaggctac cgcgctcttg atgtcagact
      301 ccctgaacag ttctcgataa tcgggaaact cagagcgcat tatcctgtgg tcacactctg
      361 ccatgtgttc ggggttcatc gcagcagcta cagatactgg aaaaaccgtc ctgaaaaacc
      421 agacggcaga cgggctgtat tacgcagtca ggtacttgag ctacatggca tcagccacgg
      481 ttcggccgga gcaagaagca tcgccacaat ggcaacccgg agaggctacc agatgggacg
      541 ctggcttgct ggcaggctca tgaaagagct ggggctggtc agctgtcagc agccgactca
      601 ccggtataaa cgtggtggtc atgaacatgt tgctatccct aactaccttg aaaggcagtt
      661 cgccgtgacc gagccaaatc aggtgtggtg cggtgatgtg acctatatct ggacgggtaa
      721 gcgctgggcg tacctcgccg ttgttctcga cctgttcgca agaaaaccag tgggctgggc
      781 catgtcgttc tcgccggaca gcaggctcac catgaaagcg ctggaaatgg catgggaaac
      841 ccgtggtaag cccggcgggg tgatgttcca cagcgatcag ggcagtcatt atacgagcag
      901 g
//
LOCUS       DAJTRD010000293          864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTRD010000293 DAJTRD010000000
VERSION     DAJTRD010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.300"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..257)
                     /locus_tag="OO315_004355"
     CDS             complement(<1..257)
                     /locus_tag="OO315_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892984.1"
                     /translation="MSLQVSHYNMLRASHEGSQKVVVRTVITVRFVPGAAIAKSILYC
                     AGQLFFKESGHHLTGTDTIYFVTVRLHMHNSTYSSIFCFSMS"
     gene            282..521
                     /gene="relB"
                     /locus_tag="OO315_004356"
     CDS             282..521
                     /gene="relB"
                     /locus_tag="OO315_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5892985.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            521..808
                     /gene="relE"
                     /locus_tag="OO315_004357"
     CDS             521..808
                     /gene="relE"
                     /locus_tag="OO315_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5892986.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          240 a          175 c          220 g          229 t
ORIGIN      
        1 gacatgctaa agcaaaaaat agatgaataa gttgagttgt gcatatgtag cctgaccgtc
       61 acaaagtata tggtgtctgt accagtaaga tgatggccgg actctttaaa aaagagctga
      121 cctgcacaat acaggatgga cttagcaatg gctgctcctg gcacaaagcg gacagtgatc
      181 accgttctta cgactacttt ctgacttcct tcgtgacttg ccctaagcat gttgtagtgc
      241 gatacttgta atgacatttg taattacaag aggtgtaaga catgggtagc attaacctgc
      301 gtattgacga tgaacttaaa gcgcgttctt acgccgcgct tgaaaaaatg ggtgtaactc
      361 cttctgaagc gcttcgtctc atgctcgagt atatcgctga caatgaacgc ttgccgttca
      421 aacagacact cctgagtgat gaagatgctg aacttgtgga gatagtgaaa gaacggcttc
      481 gtaatcctaa gccagtacgt gtgacgctgg atgaactctg atggcgtatt ttctggattt
      541 tgacgagcgg gcactaaagg aatggcgaaa gctgggctcg acggtacgtg aacagttgaa
      601 aaagaagctg gttgaagtac ttgagtcacc ccggattgaa gcaaacaagc tccgtggtat
      661 gcctgattgt tacaagatta agctccggtc ttcaggctat cgccttgtat accaggttat
      721 agacgagaaa gttgtcgttt tcgtgatttc tgttgggaaa agagaacgct cggaagtata
      781 tagcgaggcg gtcaaacgca ttctctgaac caaagcatga catctctgtt tcgcaccgaa
      841 ggtgacactt ctgctttgcg ttga
//
LOCUS       DAJTRD010000294          863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTRD010000294 DAJTRD010000000
VERSION     DAJTRD010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.301"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..271)
                     /locus_tag="OO315_004358"
     CDS             complement(<1..271)
                     /locus_tag="OO315_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5892987.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(365..863)
                     /locus_tag="OO315_004359"
                     /pseudo
     CDS             complement(365..863)
                     /locus_tag="OO315_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628301.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
BASE COUNT          255 a          174 c          195 g          239 t
ORIGIN      
        1 tacaccagac atcgttatta agtagcagca ccataagata aggagtggca tcgttagcca
       61 cagcttccct actccagagg taatataaag gggtgggctc gactgattta tctttacgtc
      121 gcttacactg caaatgttca gaaattagtc tatgcagctc accagtaaaa tcagcaatca
      181 gagaaggaat tgccttattc ataccagggc aaggtattaa tttaaactgt aataatttaa
      241 ttttaggatg tgtagcagca gcccgataca gagttgcaag gacacacttt tgccagaggg
      301 cgttacagga aagcttaatg tttgattctg tatacataat aaatcacctt atagttacaa
      361 taggtcaaaa accgctgtag ccaaagttac gctggcctga tgctttagtg tcgagcttcg
      421 tcaaataatc cagacgtacc aataagcgct ggtactgctc tgggaaatta ggatcgttaa
      481 tatccaggat gtaacgtcca ttagcaggga aatgaactaa tccctggtga tcttccagtt
      541 ccaaccctaa ggcactgtac caggctccgg taattaaccc tctcagggtg tcagagcgct
      601 catagtttcc cagatggtaa taggcgtctt tgttaaaaaa aggcagacgt gataatgaca
      661 tttaaaggac tgcgtatatt ctttcgccca gataatacaa accgacgttg cgtaggtgcg
      721 tttgccttgt tgttttttac ggtgttgatc tgcattaatt ttcgcattca gcgagttacg
      781 gaaacgagat atctctccgg ggatcaggtt agggaaacag gtaatgatat ccccgttgtc
      841 aatcacagga gggtaatgga gat
//
LOCUS       DAJTRD010000295          863 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTRD010000295 DAJTRD010000000
VERSION     DAJTRD010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.302"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..773
                     /locus_tag="OO315_004360"
                     /pseudo
     CDS             1..773
                     /locus_tag="OO315_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
BASE COUNT          256 a          202 c          204 g          201 t
ORIGIN      
        1 ttgccgaatg gttttcacaa aaacgtccgg cggcgatgat gatcggcatc cgtgcggatg
       61 agtcctacaa ccgttttgtc gccatcgcca gtttaaataa acaacgtttt gccgacgata
      121 aaccatggac tacggccgca ccaggcggtc atagctggta catttacccc atttacgact
      181 ggaaagtggc tgatatctgg acctgatatg caaatcatca aaatctctgc aacccactgt
      241 ataacctaat gtatcaggca ggcgttcctc tgcgtcatat gcgaatttgc gaaccttttg
      301 gcccggagca acgacaagga ttatggctct gtcacgttat cgaaccggat cgctgggctg
      361 ctatgtgcgc acgagtcagc ggggtaaaaa gtggcggcat ttacgccgga catgacaatc
      421 atttctatgg gcatcggaaa atcctcaaac cagaacattt agactggcaa gaatatgcat
      481 tattgctgct caatagcatg ccggaaaaaa cagctgagca ttaccgcaat aaaatcgcca
      541 tttatttgca ctggtatcag aaaaaaggca tcgaggttcc acaaacccag caaggggaca
      601 ttggcgcgaa agatatcccc tcctggcggc ggatatgcaa agttttactc aataacgatt
      661 actggtgtcg ggcattatca tttagtccta cgaaagcgaa gaactatcag cgttataacg
      721 aacggataaa aggaaaacgt caggaatggg ggatactatg caacaacgat taacgcagga
      781 tttaacgcaa tttctcgcca gcttgccgga agatgatcgc atcaaagcga ttaatgaaat
      841 ccgcatggcg atccatcagg tta
//
LOCUS       DAJTRD010000296          823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTRD010000296 DAJTRD010000000
VERSION     DAJTRD010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.303"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            119..>823
                     /locus_tag="OO315_004361"
     CDS             119..>823
                     /locus_tag="OO315_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5892988.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPYLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVN"
BASE COUNT          175 a          222 c          246 g          180 t
ORIGIN      
        1 tcgcccgctg cacggagcgg gaatttgcga ccattcttgc cggtatcagc gccggcggta
       61 tgtactgccc ggagtgtggc acggttcact ggccggacgg agtcgccccg cccttctgat
      121 gctcccccgt tttgccgata tttttcagca gggaaaccgc tggcttaact ggctggagaa
      181 acaaccggaa ggggcagtcc gtccggtggt aattgaatca gtgacaaaaa tcatggcctg
      241 cgggaccaca ctgatgggct acacgcagtg gtgttgttca tcaccggact gttgccatac
      301 aaaaaaggtc tgcttccggt gtaaaagccg ctcctgcccg cactgcgggg tgaaggctgg
      361 cgcacagtgg atacagtatc tgctgagcct ggtcccctac tgcccgtggc agcatattgt
      421 gttcacactt ccctgccagt actggtccct ggtgttccac aaccggtggt tactggcaga
      481 gatgagccgc attgcagcgg atgtgatact ggaaatctgc catcaggcag atgtggagcc
      541 ggggatattc acggtgatcc acacatgggg gcgtgaccag cagtggcatc cgcatatcca
      601 tttatcgaca actgccggtg gtgtgacgtc gggccacacc tggaaaaatc ttcattttta
      661 cgcccgtaag gtgatgagca tgtggcgcta ccggataaca cggttgctgt cacggaaata
      721 cccttacctg gtgatgccgg atgcgctggc agcagaagga agcagtaaac gggaatggaa
      781 tcgcttcctg gacacgcatt accgccgtgg ctggaatgtc aac
//
LOCUS       DAJTRD010000297          818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTRD010000297 DAJTRD010000000
VERSION     DAJTRD010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.304"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            201..674
                     /locus_tag="OO315_004362"
     CDS             201..674
                     /locus_tag="OO315_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5892989.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          254 a          170 c          179 g          215 t
ORIGIN      
        1 cggcatccac gttacagacg gtcccggcaa aagtgcctga cctgacccct attagcggtt
       61 accagcgccc caccagaccc ggtgacatca ccccccggat catggacgac tgggggcagt
      121 aatcatgccg ggtctggctg gctaacaccc cattataagg ggtacgaata aagtaacggg
      181 ggggagatga accctatgca atgtcctggt taatatctca gtgtgctcat cagtgcactg
      241 ataataaaaa gacggagaca gatgccattt acgataaagt tcggagttct tatttgcttt
      301 catgtattct gaaaaaaaac aaaaatgttg ggcttattct tcatgcacca tcatttgttt
      361 ctgtgtctga aaaaatcgcc agaattgtga tggcaaatta ctcccgtaac tggagtaatt
      421 ccgagttggc cagtgcggtt ttaatgagtg aatcttcttt aaaaagaaga atgtacaaag
      481 aggtcggttc tataagtaca tttgtacata aaataaagct tacagaagct atcaggaaat
      541 tacgaaggac aaatactccc atttctgtca tatcatctga gttaggttac agttcaccat
      601 cgtatttttc taaagtcttc tttaaatatt taaagacata ccctcaaaac ataaggaaga
      661 aaaatggtcg gtaatgtcac caattaggag gacttgcaga gccgcttttt tacgatactg
      721 agatcaaaca gacataatct atgcggagat ttacagcagg gcatggttca caaatctgac
      781 agcgatgaat tatcggcact gcgggcagaa aatgctcg
//
LOCUS       DAJTRD010000298          809 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTRD010000298 DAJTRD010000000
VERSION     DAJTRD010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..809
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.305"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            121..>809
                     /locus_tag="OO315_004363"
     CDS             121..>809
                     /locus_tag="OO315_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5892990.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRCE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYRAQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHESYDNGKSGEKFLQE
                     LCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTASLRGVGMLTNINHAAMNTLLGERV
                     MDRMVMNGGRWV"
BASE COUNT          197 a          194 c          252 g          166 t
ORIGIN      
        1 aatgaatacc agggtctgtt cgcgccgaaa ggtgttcggc ctgagacgtt gctccgacag
       61 gttaacaccg tctcgttgcc ggacagtgcg atcccgccag gcttcagggg gtaacagacc
      121 atgaaaaata ttgcgacagg aggcgttctg gagcgtatcc gcagactgac cccaccacat
      181 gtaaccgccc cattcagaac ggttgcggag tggcgcgagt ggcaacttgc tgaaggccag
      241 aaacgttgcg aggagatcaa ccgcctgaat cgtcagttgc gggtggaaaa aatcctgaac
      301 cgctccggca tccagccgtt gcaccggaag tgttcgtttg cgaattaccg ggcgcagaac
      361 gacggtcagc gacatgcact gagtcaggcg aaatccatag ctgacgaact gatgacaggc
      421 tgtacgaatt ttgtgttcag cggtaagccc ggcaccggaa aaaatcatct tgcagccgcg
      481 attggcaacc ggctgatggc gaaggggcgt agcgtgatta tcgtcaccgt gtccgatgtc
      541 atgagcgtgt tgcatgagag ctacgacaac ggcaaatccg gtgaaaaatt tttacaggag
      601 ctttgtggtg ttgacctgct ggtcctggat gaaattggca tgcagcggga tacgaaaaac
      661 gagcaggtgg tactgaacca gattgttgat cgccggacgg catcgttacg cggtgtgggg
      721 atgctgacaa atattaacca tgcagcgatg aatacacttc tcggcgagcg ggtgatggat
      781 cgcatggtca tgaacggcgg gcgctgggt
//
LOCUS       DAJTRD010000299          795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTRD010000299 DAJTRD010000000
VERSION     DAJTRD010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.306"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..290
                     /locus_tag="OO315_004364"
     CDS             <1..290
                     /locus_tag="OO315_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5892991.1"
                     /translation="GAACTEQFNILGSFFTTDILSDYSHLDMGNGLLLKIFHKDGTAT
                     EFNRFSQFASFSSSSAPSVTAPFRAELSANPAETVVEGPFSKDVILKITYN"
     gene            complement(333..533)
                     /gene="glgS"
                     /locus_tag="OO315_004365"
     CDS             complement(333..533)
                     /gene="glgS"
                     /locus_tag="OO315_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HCR5892992.1"
                     /translation="MEHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
BASE COUNT          240 a          180 c          145 g          230 t
ORIGIN      
        1 ctggtgcggc ctgtactgag caattcaata ttttagggag ctttttcact acggatattt
       61 tgagtgatta tagccatctg gatatgggta atggactgct attgaagata tttcataaag
      121 atggaacagc aacggaattt aaccgcttct cacaatttgc ttctttttca tcgtctagtg
      181 caccttcggt caccgcacca ttcagggcag aacttagtgc gaacccggca gagactgttg
      241 ttgagggacc gtttagtaaa gacgtaatcc tcaaaatcac ctataactag catctaatac
      301 aaacactaaa acgggccatc aggcccgtta tatcagtgct ctaactccag ctctcttgcc
      361 tgcatcatct gttggcaata ccaggcataa cgtgcgcaaa accaagttct atgttcttca
      421 tccatgttac cagtaacagc cagaatatcg accgggcgac cttctgcgtg cattctggca
      481 aaactacgcg ccaggaaatc gaaattattc aaagaattaa gactatgctc cataagcaca
      541 tcctccggtt atcgcatacc ttttaaggcc atatgctttt tctgatttca gtattcccca
      601 atcagtgtag tgcctttaaa cataacgtgc gtaaatatat tgtccccgat cacacttttt
      661 agtgaaaaat aaaaaaccat attaaaatac tttctcttcc agaacttctc taacatgtgt
      721 tattattaac aaagacaaat gtaagcaggg aaactattcc aaggtaaata ttgcgtcact
      781 aaatggacat tggag
//
LOCUS       DAJTRD010000300          763 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTRD010000300 DAJTRD010000000
VERSION     DAJTRD010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.307"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            144..641
                     /locus_tag="OO315_004366"
                     /pseudo
     CDS             144..641
                     /locus_tag="OO315_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            682..763
                     /locus_tag="OO315_004367"
                     /pseudo
     CDS             682..763
                     /locus_tag="OO315_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          196 a          189 c          223 g          155 t
ORIGIN      
        1 cttattatgc ttccatcgga aacgatgatc tggcagcctg agttcacaga taaaacactc
       61 tccaggaaac ccggggcggt tcaaagcgcc agccccgtcc cgcccgatag agcgcggcat
      121 agcgggcccc ggattacttg cgtgcgacgg cagaagaagc cctgaagcga atcagtgaac
      181 tgtacgccat cgaggatgaa atacggggat taccagagtc agagtgtctt gcagtcaggc
      241 agcagcgaag caaagcgtta ctgacgtcgc tgcatgaatg gatggtggag aagaatggca
      301 cgctgtcgaa aaaatccaga ctgggcgaag cattcagcta tgtactgaat cagtgggacg
      361 ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca gcggaaagag
      421 cgcttcgtgc agtctgtctc ggaaagaaaa attacgtgtt cttcggtagc gatcacggcg
      481 gcgagcgtgg tgcactgctg tacgggctga tcggcacctg ccgtctgaac ggtatcgatc
      541 cggaagcgta tctgcgccat attctgagcg tactgccgga atggccttcc aaccgagttg
      601 acgacctcct gccatggaag gtggtgctac catcaggata atcgtcaata cggtgttcgg
      661 tgaacgttta cttggagcat catgcttcat gaagaaaaac tggcacgtca tcaacgtaaa
      721 caggcgatgt atacccgaat ggcagccttc ccggcggtga aaa
//
LOCUS       DAJTRD010000301          753 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTRD010000301 DAJTRD010000000
VERSION     DAJTRD010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.308"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..730
                     /locus_tag="OO315_004368"
     CDS             <1..730
                     /locus_tag="OO315_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCR5892993.1"
                     /translation="TDRIHLKDAARMLEVSVMTIRRDLHQEDEPLPLTLLGGYIVMVN
                     KPAPSMPVIHDVPKNHRDDLPIAILAAGMVNENDLIFFDNGQEIPLVISMIPDAITFT
                     GICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLNPRKIFISASGV
                     HNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPISAFDVLISDRP
                     LPADYVTHCQNGSVKIITPDSEDE"
BASE COUNT          202 a          184 c          161 g          206 t
ORIGIN      
        1 aaccgacaga atccatttga aagacgcagc gcgaatgctg gaagtttctg taatgactat
       61 tcgtcgcgat ctccatcagg aagatgaacc tctgccactg accctactgg gtggctatat
      121 tgtaatggtg aataaacccg cgccatccat gccagtaatc catgacgttc caaaaaatca
      181 tcgtgatgac ttacctattg caattctggc tgccggaatg gttaatgaaa atgatctgat
      241 cttctttgat aatggccagg agataccact cgttataagc atgatcccgg atgcaatcac
      301 cttcaccggc atctgttact cacatcgcgt ctttgttgcg ttgaatgaaa agcctaatgt
      361 aacagcaata ctttgtggtg gtacgtatcg tgccagaagt gatgcttttt acgatgccag
      421 taactcttcg ccattagact ctctcaatcc gcgaaaaata tttatttccg ccagcggtgt
      481 gcataatcac tttggcgtca gctggtttaa ccctgaagat cttgccacta agcgtaaagt
      541 gatgaaccgt ggactacgga aaattttgct cgcccgccac gcgttgttcg atgaagtggc
      601 ctctgccagc ctcgcaccga tctctgcatt tgacgttctg attagcgatc gtccgttacc
      661 ggcagattat gttacgcact gccagaatgg ttctgtaaag atcattacac ctgattcaga
      721 agacgaatga cttactgaaa aaacaccaca atc
//
LOCUS       DAJTRD010000302          753 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTRD010000302 DAJTRD010000000
VERSION     DAJTRD010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.309"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            459..>753
                     /locus_tag="OO315_004369"
     CDS             459..>753
                     /locus_tag="OO315_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5892994.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDL"
BASE COUNT          256 a          151 c          124 g          222 t
ORIGIN      
        1 tgttgaaaca aatgacggta gagaatgtta acactgccgt taaacaatat ctttctcatc
       61 cggtaaatac ttataccgga gtattattgc caaaataata acagccccgt caacacatcg
      121 ttggcgggga ttttagcaat attcgctatt tttatgtaat aattttataa atgcgttcaa
      181 aataataacc aagtactaat agtgatattt taatacctga tttttacgtg ataattcagg
      241 aggcacagaa agcgcataaa aataacaaca taaaacacct tgccaccacc caagaattct
      301 ctattacatt gtttttaatg aaaaaatatc attccaacaa tatctcacga taaataacat
      361 taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg ataaatccta
      421 cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag caagtaacgg
      481 atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc actatcgcag
      541 aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcatttcag attatcaatg
      601 atgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc cagacctggg
      661 acgacgaaaa cgtccacaag ttgatggatt tatccattaa caaaaactgg atcgacaaag
      721 aagaatatcc gcaatccgca gccatcgacc tgc
//
LOCUS       DAJTRD010000303          733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTRD010000303 DAJTRD010000000
VERSION     DAJTRD010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.310"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..461
                     /locus_tag="OO315_004370"
     CDS             <1..461
                     /locus_tag="OO315_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5892995.1"
                     /translation="TVVLQRDAYQTGQHRFHPSLWQFGKEMGFSPRLCRPFRAQTKGK
                     VERMVQYTRNSFYIPLMTRLRPMGSTVDVETANRHGLRWLHDVANQRKHETIQARPCD
                     RWLEEQQSMLALPPEKKEYDVHPGENLVSFDNPPQHHPLSIYDSFCRGVA"
     gene            458..>733
                     /gene="istB"
                     /locus_tag="OO315_004371"
     CDS             458..>733
                     /gene="istB"
                     /locus_tag="OO315_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5892996.1"
                     /translation="MMMELQHQRLMALAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQ"
BASE COUNT          187 a          209 c          193 g          144 t
ORIGIN      
        1 aaactgtggt tctgcaacgt gacgcatatc agaccggtca gcaccggttc catccttcgc
       61 tgtggcagtt cggcaaggag atgggcttct ctccccgact gtgtcgcccc ttcagggcac
      121 agactaaagg taaggtggaa cggatggtgc agtacacccg taacagtttt tacatcccac
      181 taatgactcg cctgcgcccg atggggagca ctgtcgatgt tgaaacagcc aaccgccacg
      241 gtctgcgctg gctgcacgat gtcgctaacc aacgaaagca tgaaacaatc caggcccgtc
      301 cctgtgatcg ctggctcgaa gagcagcagt ccatgctggc actgcctccg gagaaaaaag
      361 agtatgacgt gcatcctggt gaaaatctgg tgagcttcga caaccccccc cagcatcatc
      421 cactctccat ctacgactca ttctgcagag gagtggcgtg atgatggaac tgcaacatca
      481 acgactgatg gcgctcgccg ggcagttgca actggaaagc cttataagcg cagcgcctgc
      541 gctgtcacaa caggcagtag accaggaatg gagttatatg gacttcctgg agcatctgct
      601 tcatgaagaa aaactggcac gtcatcaacg taaacaggcg atgtataccc gaatggcagc
      661 cttcccggcg gtgaaaacgt tcgaagagta tgacttcaca ttcgccaccg gagcaccgca
      721 gaagcaactc cag
//
LOCUS       DAJTRD010000304          713 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTRD010000304 DAJTRD010000000
VERSION     DAJTRD010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..713
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.311"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            157..>713
                     /locus_tag="OO315_004372"
     CDS             157..>713
                     /locus_tag="OO315_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5892997.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDS"
BASE COUNT          252 a          131 c          128 g          202 t
ORIGIN      
        1 gggtctggct ggctaacacc ccattataag gggtacgaat aaagtaacgg gggaagcaat
       61 acctggagaa agagtacaaa gcaaactgta ttaaacttaa taagttatat tgtatttcac
      121 tagagttctt atcgttgata agaaattctg gttataatga ttaaatcaac gaatatacag
      181 gtaatcggtt ctggtattat gcatcaaata aacaacatac actcgttaac tctattttct
      241 ttgcctgtga gtttgagtcc atcttgtaat gaatattatt taaaagtctg gagtgaatgg
      301 gaaaagaacg gtaccccagg cgagcaacgc aatatcgcct tcaataggct aaaaatatgt
      361 ttgcaaaatc aaggggcgga attaaatcta tctgagttag atttaaaaac attacctgat
      421 ttaccgcctc agataacaac actggaaata agaaaaaacc tattaacata tctccctgat
      481 ttcccaccaa tgcttaaggt aatacatgct caatttaatc aactggaaag cttacctgcc
      541 ttacccgaga cgttagaaga gcttaatgtg ggtgataaca agataaaaga attaccatat
      601 cttcctgaaa ctctaactca tttacggatc cataataacc gattgcatat tctgccacta
      661 ttgccaccgg agctaaaatt actgatagtt tctggaaaca gattagacag cat
//
LOCUS       DAJTRD010000305          700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTRD010000305 DAJTRD010000000
VERSION     DAJTRD010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.312"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..602)
                     /locus_tag="OO315_004373"
     CDS             complement(<1..602)
                     /locus_tag="OO315_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5892998.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDST"
BASE COUNT          164 a          169 c          179 g          188 t
ORIGIN      
        1 gtagagtcgc ctatagttga gtcatcagta aggttattat catcaatcag cttaatcaga
       61 ccagaggtat caccagtgtc gactctctgc tgtgctgcgg tcaggacttc cagcgctaac
      121 cttatgccct tctgctggtc cagactcaag gcttgcgtag cggctagctc cttcaggttg
      181 gacttctcgt cgaccagagt agcgacctca agtccattcc agtaatcgtc actagcgtct
      241 gtcaggaact gctcagccat agaccggaga tgctctgtaa gctcctgtag tgagacttca
      301 ggcctatcca acatgaaggc cttagcggtg gctgcaagct ctctctgacg cattgtggct
      361 actctcctgt ctgtgctgcg ggtcgatact gaaagaaacg gtgtggtctg tcccttgagt
      421 ctgaagcgga tagtatatcg accattggct ctgcggacta actgaggctt agctacgctg
      481 agtagattga tttgtgtcag ccttgtgtca gggcttgagg tgtagacttg ctgataccga
      541 tagggagacc cagagaaaga actggatgga tatacagtgt tatcatgatt aatgtcagtc
      601 atatcaggac cttaagtagc attgagtcaa ctacttcaag tccatccatc cgtctggcaa
      661 tggagtccat ctggaactac gcgggaaata ccctcgttaa
//
LOCUS       DAJTRD010000306          700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTRD010000306 DAJTRD010000000
VERSION     DAJTRD010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.313"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(74..262)
                     /locus_tag="OO315_004374"
     CDS             complement(74..262)
                     /locus_tag="OO315_004374"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5892999.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
BASE COUNT          172 a          138 c          168 g          222 t
ORIGIN      
        1 tataggggca aatccactac cgcgctgatg gttcttatcc gttttattga atcggagaac
       61 aaactgtgag ctatcagcct ggtggtctgg ttttgcctga tttaagccgc cgtgggtatt
      121 tgtccaagaa tgcttcaagc tccaatcgtt ttggcctggt gaatggagat gggttaagca
      181 aagactctcg ttgctggtgt actttttcgc gctggcgctc aagcatggat aatgcgattg
      241 cctgtagttc ttcgttagtc attgtctgca cctggaaagc aatttaccgg agtacctcac
      301 actacggcaa aaatgtttta ttgttgcaac ttgcgtaatt attcaggtca gtttggaacg
      361 cactgtataa acataaataa cctgatactg ttgtgtgttt tcaaagaaaa cacaggtatt
      421 tttcacataa accttcccct tttcatattc gaccacttaa tcaaaaaacg gttttatact
      481 tgttgccccc actggggtta aatgtgagac atgtcgcact ttgtgccatg tgtcctcgct
      541 gattttaggc gcggttcatt cattctttct gttggcataa agtatggtat cggttgggcg
      601 aatgaaaggg actcgggtca tggtcagaca atacctgagg cgttaattcg ttgaaagaga
      661 ttgtgaatgg gggaataact gagtcttaac tgctgtgcga
//
LOCUS       DAJTRD010000307          697 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTRD010000307 DAJTRD010000000
VERSION     DAJTRD010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.314"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..>697
                     /locus_tag="OO315_004375"
     CDS             <1..>697
                     /locus_tag="OO315_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HCR5893000.1"
                     /translation="HQIVNRLDIMLPGNRPFVAPSAVIQARQRLGSEAVRRVFTKTAQ
                     LWHNATPHPHWCGLTLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNGWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCH
                     LLTSMTDAMRFPGG"
BASE COUNT          175 a          203 c          199 g          120 t
ORIGIN      
        1 tcaccagatt gtgaatcgcc tggacatcat gctgccgggc aatcgcccct tcgttgcccc
       61 cagtgccgtt attcaggccc gccagcgcct gggaagtgag gctgtccgcc gcgtgttcac
      121 gaaaacagcg cagctctggc ataacgccac gccgcatccg cactggtgcg gcctgaccct
      181 gctggccatc gatggtgtgt tctggcgcac accggataca ccagagaacg atgcagcctt
      241 cccccgccag acacatgccg ggaacccggc gctctacccg caggtcaaaa tggtctgcca
      301 gatggaactg accagccatc tgctgacggc tgcagccttc ggcacgatga agaacagcga
      361 aaatgagctt gctgagcaac ttatagaaca aaccggcgat aacactctga cgttaatgga
      421 taaaggttat tactcactgg gactgttaaa tggctggagc ctggcgggag aacaccgcca
      481 ctggatgata cctctcagaa agggagcgca atatgaagag ctcagaaaac tgggtaaagg
      541 cgatcatctg gtgaagctga aaaccagccc gcaggcacga aaaaagtggc cgggactggg
      601 aaatgaagtg acagcccgcc tgctgaccgt gacgcgcaaa ggaaaagtct gccatctgct
      661 gacgtcgatg acggacgcca tgcgcttccc cggagga
//
LOCUS       DAJTRD010000308          691 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTRD010000308 DAJTRD010000000
VERSION     DAJTRD010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.315"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            219..>691
                     /locus_tag="OO315_004376"
     CDS             219..>691
                     /locus_tag="OO315_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR5893001.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT          189 a          141 c          163 g          198 t
ORIGIN      
        1 aagctgtttt ccttgccaac aatcttgcaa aaaaaatgcg attgctaaaa caaaagtgca
       61 atttgcggca atatagttat tccatgaccg tacaatcttg cattcagtgt aaataatctt
      121 gtttagctct cgctaaacgc ggttgtataa ccgcaataca cgccgtattt tatccggcat
      181 atattgtttt tgcttgctca tggaataggg aggttgtcat gtatcaacgc tgttttgata
      241 atgccagtga aacgctgttt gtcgccggta aaacgccacg gctttcacgt tttgcattta
      301 gcgatgatcc aaaatgggag tctggacatc acgttcatga caatgaaacc gagctgattt
      361 acgtcaagaa aggggttgca aggtttacca tcgattcttc gttatatgtc gcacatgcgg
      421 atgacattgt ggtgatagaa cgcggcaggc tgcatgcggt ggcctctgac gttaacgatc
      481 cggcaacgac gtgtacctgt gcgctgtacg gctttcagtt tcagggggct gaggaaaatc
      541 agttactgca accgcattct tgtccggtaa ttgccgcggg gcagggaaaa gaagtcataa
      601 aaaccttatt taatgagcta agtgtgattt tgccgcaaag taaaaatagc caaacatctt
      661 cgttatggga tgcattagcc tatacattag c
//
LOCUS       DAJTRD010000309          685 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTRD010000309 DAJTRD010000000
VERSION     DAJTRD010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 685)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..685
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.316"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            304..>685
                     /locus_tag="OO315_004377"
     CDS             304..>685
                     /locus_tag="OO315_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR5893002.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT          187 a          151 c          165 g          182 t
ORIGIN      
        1 tcttcccgcg ctttttctag tcggtgaggt gtgggggctt ctgttttgtc gtcgctctcg
       61 tcagacacgt cgccaatcct ggaaagagta ttaagcgtaa atgatgccag agcgcaaagc
      121 gttcaatggt ttaagtaagg ggcaaaacag gcgggattta gggcgtttat gcaaaaatag
      181 gttcagttgc agaacgtttt gcgaaccatt ttccagttct gatatttagc catttagcct
      241 atagaatcgc actccgcctc tctgttagtt tgctaataat taaattatta caaaggtgat
      301 caaatggaat ggcaacaatt gcttaactgc aaccgtcgga aagataatct aaaaaaaacc
      361 aacatagcat ttttacatcc ccttgctagc ggaagacaag agatcgagcg ggattacgac
      421 cgattacttt ttgctgcacc aactcgtcgg cttgcggata aaacacaagt tttcccgtta
      481 gatcaaaatg acagcatacg gacgaggctg actcattctc acgaagtagc aaactttgct
      541 cgcgggatag gaatgaggct cgctttcgag atgaaggagc acatctttgg cgaaataccc
      601 acggggattg tcgttgaacg cgacgttcct gctctgttag cagcgatcgg actggcacac
      661 gatctcggta atccaccatt tgggc
//
LOCUS       DAJTRD010000310          668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTRD010000310 DAJTRD010000000
VERSION     DAJTRD010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.317"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..>668
                     /locus_tag="OO315_004378"
     CDS             <1..>668
                     /locus_tag="OO315_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR5893003.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAJTRD010000311          649 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTRD010000311 DAJTRD010000000
VERSION     DAJTRD010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.318"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(24..>649)
                     /locus_tag="OO315_004379"
     rRNA            complement(24..>649)
                     /locus_tag="OO315_004379"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          142 a          193 c          153 g          161 t
ORIGIN      
        1 ctacaaagaa cgcttcttta aggtaaggag gtgatccaac cgcaggttcc cctacggtta
       61 ccttgttacg acttcacccc agtcatgaat cacaaagtgg taagcgccct cccgaaggtt
      121 aagctaccta cttcttttgc aacccactcc catggtgtga cgggcggtgt gtacaaggcc
      181 cgggaacgta ttcaccgtgg cattctgatc cacgattact agcgattccg acttcatgga
      241 gtcgagttgc agactccaat ccggactacg acgcacttta tgaggtccgc ttgctctcgc
      301 gaggtcgctt ctctttgtat gcgccattgt agcacgtgtg tagccctggt cgtaagggcc
      361 atgatgactt gacgtcatcc ccaccttcct ccagtttatc actggcagtc tcctttgagt
      421 tcccggccgg accgctggca acaaaggata agggttgcgc tcgttgcggg acttaaccca
      481 acatttcaca acacgagctg acgacagcca tgcagcacct gtctcacggt tcccgaaggc
      541 acattctcat ctctgaaaac ttccgtggat gtcaagacca ggtaaggttc ttcgcgttgc
      601 atcgaattaa accacatgct ccaccgcttg tgcgggcccc cgtcaattc
//
LOCUS       DAJTRD010000312          646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTRD010000312 DAJTRD010000000
VERSION     DAJTRD010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.319"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..615)
                     /gene="aqpZ"
                     /locus_tag="OO315_004380"
     CDS             complement(<1..615)
                     /gene="aqpZ"
                     /locus_tag="OO315_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5893004.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQL"
BASE COUNT          202 a          189 c          143 g          112 t
ORIGIN      
        1 cagttgttct aatgcccagc cgccctggaa gatagcaacc gcggtgctgc gcgccgggtt
       61 aacagaagtg ttagtcaccg gaatactgat taagtgaatc agggttaagg ccagaccaat
      121 agcgatcggc gcaaaacctg ccggagcgaa tttgtcggtt gcaccgtgga tcactaacag
      181 gaagcccgca ctcaatacca gttcaactac cagcgcggaa agcatggaat aaccgcctgg
      241 tgaatgctcg ccataaccgt tagaagcaaa accgctggct gccgcgtcaa aacccgtttt
      301 accactggca attaaataca gcagcgccgc tgcaacaata ccgccgacaa cctgggcaat
      361 tacgtagcca acgacttctt ttgccggaaa acgtccgcca gcccataaac caatagtgac
      421 cgccgggtta aaatgaccac cagaaatatg accaacagcg aaggccatcg tcagaacggt
      481 caaaccaaat gccagcgcca cgccggcaaa accaatgcct aattccggga agcctgcggc
      541 cagtacagca ctaccacagc caccaaaaac aagccagaaa gtaccaaaac attcagctgc
      601 taattttctg aacatatcca cctcaattaa aaattgaccc tgtgaa
//
LOCUS       DAJTRD010000313          617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTRD010000313 DAJTRD010000000
VERSION     DAJTRD010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.320"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..>617
                     /locus_tag="OO315_004381"
     CDS             <1..>617
                     /locus_tag="OO315_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5893005.1"
                     /translation="KYNRLLSQRVAAYASEINRLKALVAKLQRMQFGKSSEKLRAKTE
                     RQILEAQERISALQEEMAETLGEQYDPVLPSPLRQSSARKPLPASLPRETRVIRPEEE
                     CCPACGGELSSLGCDVSEQLELISSAFKVIETQRPKLACCRCDHIVQAPVPSKPIARS
                     YAGAGLLAHVVAGKYADYLPLYRQSEIYRRQGVELSRATLGRWTGA"
BASE COUNT          146 a          172 c          176 g          123 t
ORIGIN      
        1 aaatacaacc gcctgctctc acagcgggtg gcggcttatg cttccgaaat caaccggctg
       61 aaggcgctgg ttgcgaaact gcagcgcatg cagttcggta aaagctcaga aaaacttcgc
      121 gcaaaaaccg aacggcagat actggaagca caggagcgaa tcagcgcact tcaggaagaa
      181 atggcggaaa cgctgggtga gcaatatgac ccggtactgc catctcccct gcgccagtct
      241 tcagcccgta aaccgttacc tgcctcactt ccccgtgaaa cccgggtcat ccgtccggaa
      301 gaggaatgct gcccggcctg tggtggcgaa ctcagttctc tgggatgtga tgtgtcagag
      361 cagctggagc ttatcagcag cgcctttaag gttatcgaaa cacaacgtcc gaaactggcc
      421 tgttgccggt gtgaccatat cgtgcaggca ccggtgcctt caaaacccat tgcacgcagt
      481 tatgccggag cggggcttct ggcccatgtt gttgctggaa aatatgcaga ctatctgccg
      541 ttataccgcc agtcagaaat ataccgtcgt cagggcgtgg agctgagccg tgccacactg
      601 gggcgctgga caggtgc
//
LOCUS       DAJTRD010000314          614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTRD010000314 DAJTRD010000000
VERSION     DAJTRD010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.321"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            165..>614
                     /locus_tag="OO315_004382"
     CDS             165..>614
                     /locus_tag="OO315_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005062103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5893006.1"
                     /translation="MAGVLEGRLSGGTAAEFCNSAVVALQAAGLDVCREYPVPERGDG
                     CGGRIDIVVTDRNGVRCGIELDRNSPRQKSLLKIGAVETGICVLRRSDIARHTEQGIL
                     VIGGAVRQKKFDPLSVDLPDWLSETLWHEWVQFRQALRKPIRTELGAN"
BASE COUNT          149 a          128 c          187 g          150 t
ORIGIN      
        1 gtattaagac tatcacttaa ataagtgata ctggttgtct ggagattcag ggggccagtc
       61 tagatgccaa cacaaaggca actaataaag atacagataa agatctaaaa acagatctaa
      121 cccatcccaa acccttccct tccggaaggg agtttcggga ttttgtggct ggagtgcttg
      181 aggggagatt atctggcggt actgcagcgg aattttgtaa ttctgcggtg gttgcgttgc
      241 aggctgctgg cctggatgtc tgtcgtgagt atccggtgcc agagcgtggt gacggttgcg
      301 gagggcggat tgatatcgtc gtgactgaca ggaacggtgt ccggtgtggg atcgagcttg
      361 accgaaattc tccgcgacag aaatcactgc tcaaaatcgg tgctgttgaa accgggatat
      421 gtgtcttgcg gcgcagtgat atcgcaaggc acaccgagca gggaattctg gttatcggtg
      481 gggctgttcg ccagaaaaaa tttgacccgt tgtcagttga tctgcccgac tggttgtcag
      541 aaacactctg gcatgagtgg gtccagttca ggcaggcatt gcgaaaaccg attcgaacgg
      601 agctgggcgc taac
//
LOCUS       DAJTRD010000315          605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTRD010000315 DAJTRD010000000
VERSION     DAJTRD010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.322"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..159)
                     /locus_tag="OO315_004383"
     CDS             complement(<1..159)
                     /locus_tag="OO315_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5893007.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEGASRLSVPEGTLGQW
                     VTAARKGLN"
     gene            complement(497..>605)
                     /locus_tag="OO315_004384"
     CDS             complement(497..>605)
                     /locus_tag="OO315_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5893008.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
BASE COUNT          155 a          141 c          128 g          181 t
ORIGIN      
        1 attgagccct tttcttgcgg cggtaaccca ttgtcctaaa gtgccttcag gaacagataa
       61 tcgggaagcg ccttcactga tcgaaagctg attttcagga accgttttga cggcttcggc
      121 tttaaactcc gtagagtaac gttgggtttt tctgctcatt attggctcct tctgatgcca
      181 ttctatttca ggaaggagtg tccgttaaac tcaggctacc tcactcccgt aaagacagga
      241 gtcaagttcc cgctccgaca taactgcgtg caatggattt tgaaggaacc gtcagcccat
      301 tccccggagg caaacatggg ctggcaaaag tgtagcggta ttaggcacag ctatttagga
      361 aagagggacc atctccatct cattaaatgg accacgccag acaattcact gtcatgctga
      421 tgctgcactg tggggacaga atcactcatg atgacttcga taatcgacga cattattatt
      481 tggccgagct tttaggtcat atctcaaatc gtttgcctaa tattgctcca ttttttaata
      541 aaaaatcaat catttttgaa tctttgcttt tcattgcatt gtcaagcata gtgtccccac
      601 tattt
//
LOCUS       DAJTRD010000316          600 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTRD010000316 DAJTRD010000000
VERSION     DAJTRD010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..600
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.323"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(55..130)
                     /locus_tag="OO315_004385"
     tRNA            complement(55..130)
                     /locus_tag="OO315_004385"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(95..97),aa:Thr,seq:tgt)
     gene            complement(133..209)
                     /locus_tag="OO315_004386"
     tRNA            complement(133..209)
                     /locus_tag="OO315_004386"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(173..175),aa:Arg,seq:tct)
     gene            complement(211..287)
                     /locus_tag="OO315_004387"
     tRNA            complement(211..287)
                     /locus_tag="OO315_004387"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(251..253),aa:Ile,seq:cat)
     gene            complement(477..>600)
                     /locus_tag="OO315_004388"
     CDS             complement(477..>600)
                     /locus_tag="OO315_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5893009.1"
                     /translation="PKWSLRTCERRIEHWLKVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          157 a          154 c          133 g          156 t
ORIGIN      
        1 ccgcatcatc agcttggaag gctgaggtaa tagccattat acgatgcccg catatggtgc
       61 cgactaccgg aatcgaactg gtgacctact gattacaagt cagttgctct gcctgctgag
      121 ctaagtcggc gctggcccac caccgaggac tcgaacctcg caccgtcaac ttagaaggtt
      181 gatgctctat ccggatgagc tagtggtggt tggtggccct tgctggactt gaaccagcga
      241 cctggcgatt atgagtcgct cgctctgacc aactgagcta aagggccgga ggcagaataa
      301 taaccatatg tcatcacatc tgcaaactca tctgatcacc agcacgttta acgtcctgtg
      361 ccgtttttca ggtataaaaa aaaccgcata aagcgggctc tttcaaatgt ccatgtctgc
      421 tattcgcctc gcggtacagc tttgcgaagc gtacaagaat tgaagcagtt tttacgtcaa
      481 aaagcaataa cttttttctc tataccaaaa gccataacca ttggtttgta caaaataaat
      541 tctgccacct ttagccaatg ctcaatgcgt ctttcacagg ttcttaaact ccatttcggg
//
LOCUS       DAJTRD010000317          595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTRD010000317 DAJTRD010000000
VERSION     DAJTRD010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.324"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
BASE COUNT          219 a          104 c          122 g          150 t
ORIGIN      
        1 ttaatcccgt actctctggc cagttgggcc acgcaggccc ctagctggta agatttttca
       61 ataagatcaa gcttaaactc catagagtca tagagtaaga ggcacgaatc ctgcgggggc
      121 gaagagaatc tgacattgat agagtccatt aacgttataa gaggactcta tcatcgccaa
      181 tcatcagaca ttgaaataga cgacactgag atgacgctta caattctttt ggtttctgta
      241 ataagtaatc tgacatcccc cgtgcccaga agaggagagg gatatcaagc ccaagggtta
      301 ataattgata ctacggttaa aattgccttc agtaaaggaa acgtgattaa attcagggga
      361 tttcagagtg aaaaccatac aacatattaa aatctgttca tttagctata atacatacaa
      421 gaaagtgtcc agttagataa tgttatctaa ataaccacag ataaaaacga acataattgg
      481 gggggggggg atgaaaatac ctgaagcagt aaatcatatt aatgttcaga ataatattga
      541 tcttgttgat ggaaaaacga atccgaataa agcaacaaaa gcattacaaa aaaat
//
LOCUS       DAJTRD010000318          581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTRD010000318 DAJTRD010000000
VERSION     DAJTRD010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.325"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..>581)
                     /gene="capU"
                     /locus_tag="OO315_004389"
     CDS             complement(<1..>581)
                     /gene="capU"
                     /locus_tag="OO315_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5893010.1"
                     /translation="SPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIAFEASKFGI
                     DITFALFRNSLHIPTVWRLLGIVHSFQPDAIVCHSGHDSNIVGLVRFFTWKHPFRIIR
                     QKTYLTRKTKFFSINHFCDEVIVPGTNMKTHLEQEGCRTRVTVVPPGFDFQELYVDSR
                     NSLPPSVLSWLASRRGCPVIAQVGMLRPEKGHE"
BASE COUNT          172 a          138 c          110 g          161 t
ORIGIN      
        1 aattcgtgcc ctttttccgg gcgcaacatt cctacctgag caattacggg gcatcctctt
       61 cgggacgcca gccaggaaag aacactcgga ggcaaactgt ttcgggaatc aacgtataat
      121 tcctggaaat caaagccagg aggcacaaca gtaacccgag ttcgacatcc ttcctgctcc
      181 agatgtgtct tcatatttgt tccgggaaca atcacctcat cgcaaaaatg atttattgaa
      241 aaaaattttg tttttcgtgt caaatatgtc ttttgcctga taattctgaa tggatgtttc
      301 caggtaaaaa accgtaccaa accaacaata ttactatcat gtccactgtg acaaacgatt
      361 gcatcgggct gaaaactatg aactattccg agtaatctcc atacagtagg aatgtgaaga
      421 ctgtttcgaa ataacgcgaa tgtgatatca attccaaact tactggcttc aaaagcaatt
      481 ttgctgttct ccctgcagac aagcagaact gaatgcccca ttttcttcag ggccttcatt
      541 tgagcaacag cctgtaattc ctgaccacct atattcggtg a
//
LOCUS       DAJTRD010000319          569 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTRD010000319 DAJTRD010000000
VERSION     DAJTRD010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..569
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.326"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..569
                     /locus_tag="OO315_004390"
                     /pseudo
     CDS             1..569
                     /locus_tag="OO315_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          135 a          143 c          168 g          123 t
ORIGIN      
        1 ttcctgcctc tgaacttgct gccgccatga agcagattaa agaactccag cgcctgctcg
       61 gcaagaaaac gatggaaaat gaactcctca aagaagccgt tgaatatgga cgggcaaaaa
      121 agtggatagc gcacgcgccc ttattgcccg gggatgggga gtaagcttag tcagccgttg
      181 tctccgggtg tcgcgtgcgc agttgcacgt cattctcaga cgaaccgatg actggatgga
      241 tggccgccgc agtcgtcaca ctgatgatac ggatgtgctt ctccgtatac accatgttat
      301 cggagagctg cccacgtatg gttatcgtcg ggtatgggcg ctgcttcgca gacaggcaga
      361 acttgatggt atgcctgcga tcaatgccaa acgtgtttac cggctcatgc gccagaatgc
      421 gctgttgctt gagcgaaaac ctgctgtacc gccatcgaaa cgggcacata caggcagagt
      481 ggccgtgaaa gaaagcaatc agcgatggtg ctctgacggg ttcgagttct gctgtgataa
      541 cggagagaga ctgcgtgtca cgttcgcgc
//
LOCUS       DAJTRD010000320          555 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTRD010000320 DAJTRD010000000
VERSION     DAJTRD010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.327"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..>555
                     /locus_tag="OO315_004391"
     CDS             <1..>555
                     /locus_tag="OO315_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HCR5893011.1"
                     /translation="RTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGG
                     YLNLTHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLML
                     EPQLHYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLR
                     DSTKHRVSELPVNWWVQPSVIRTFS"
BASE COUNT          116 a          154 c          169 g          116 t
ORIGIN      
        1 agaacagagg ttgccggtat gtctctgacg acaggggtgt atggtgccgc aggccattct
       61 tccgttgatg ttaaggatga tgacggttcc cgcgccggca cggtccggga tgatgccggc
      121 agcctgggcg gatacctgaa tctgacacac acgtcctccg gcctgtgggc tgacattgtg
      181 gcacagggaa cccgccacag catgaaagcg tcatcggaca ataacgactt ccgtgcccgg
      241 ggctggggct ggctgggctc actggaaacc ggtctgccct tcagtatcac tgacaacctg
      301 atgctggagc cacaactgca ctatacctgg cagggactta ccctggatga cggccaggat
      361 aacgccggtt atgtgaagtt cgggcatggc agtgcacaac atgtgcgtgc cggtttccgt
      421 ctgggcagcc acaacgatat gagctttggt gaaggcacct catcccgtga cactctgcgc
      481 gacagtacaa aacaccgtgt gagtgaactg ccggtgaact ggtgggtaca gccttctgtt
      541 atccgcactt tcagc
//
LOCUS       DAJTRD010000321          534 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTRD010000321 DAJTRD010000000
VERSION     DAJTRD010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 534)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 534)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..534
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.328"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..>534
                     /locus_tag="OO315_004392"
     CDS             <1..>534
                     /locus_tag="OO315_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5893012.1"
                     /translation="ATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKN
                     PKRTVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPL
                     VSAFTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMT
                     ALMILITLMNSAGGKAS"
BASE COUNT          110 a          130 c          144 g          150 t
ORIGIN      
        1 ggcaacttat gcagctaact ggaatactgc ggataccact gatggtcatg cgatcattaa
       61 aagtattctg ctctgcctgt gggccttcgt gggtgttgaa tccgcagcag taagtactgg
      121 tatggttaaa aacccgaaac gtaccgttcc gctggcaacc atgctgggta ctggtttagc
      181 aggtattgtt tatatcgctg cgactcaggt gctttccggt atgtatccgt cttctgtaat
      241 ggcggcttcc ggtgctccgt ttgcaatcag tgcttcaact atcctcggta actgggctgc
      301 gccgctggtt tctgcattca ccgcctttgc gtgcctgact tctctgggct cctggatgat
      361 gttggtaggc caggcaggtg tacgtgccgc taacgacggt aacttcccga aagtttatgg
      421 tgaagtcgac agcaacggta ttccgaaaaa aggtctgctg ctggctgcag tgaaaatgac
      481 tgccctgatg atcctcatca ctctgatgaa ctctgccggt ggtaaagcat ctga
//
LOCUS       DAJTRD010000322          529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTRD010000322 DAJTRD010000000
VERSION     DAJTRD010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.329"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(160..>529)
                     /gene="pflA"
                     /locus_tag="OO315_004393"
     CDS             complement(160..>529)
                     /gene="pflA"
                     /locus_tag="OO315_004393"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="activates pyruvate formate-lyase 1 under anaerobic
                     conditions; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5893013.1"
                     /translation="DLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANKNVKVWIRY
                     VVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYKLDGVKPPK
                     KETMERVKGILEQYGHKVMF"
BASE COUNT          135 a          122 c          124 g          148 t
ORIGIN      
        1 ttcgatgggg aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat
       61 atggtagatg cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa
      121 aaagcggcaa ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc
      181 gtactgctca agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc
      241 atcgagtttg tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg
      301 gagaagttcg attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc
      361 cgaatcgtca tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt
      421 tttgttcgcc agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt
      481 ttggtggatc tcgtcgttca tctgtttgag atcgagcatt accaggtcg
//
LOCUS       DAJTRD010000323          526 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTRD010000323 DAJTRD010000000
VERSION     DAJTRD010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.330"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..>526
                     /gene="ospC2"
                     /locus_tag="OO315_004394"
     CDS             <1..>526
                     /gene="ospC2"
                     /locus_tag="OO315_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5893014.1"
                     /translation="KTDVPIFNTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPV
                     SLDRIINFVFQLEYHIPRMLSTDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAH
                     QALAYSLGNAKSDMALYLLSKFNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYK
                     VLEYFISNGLVDVNK"
BASE COUNT          200 a           63 c           96 g          167 t
ORIGIN      
        1 aaaactgatg tgccaatatt taatactaaa gatatgaggc ttggaattgg gttgcatctt
       61 attgatttta taagaaagag taaagatcaa agattccgag agttttgtta taataaaaac
      121 attgatcctg ttagtctgga tagaattata aactttgtgt ttcagcttga gtatcatata
      181 ccaagaatgc taagcacaga taacttcaaa aagataagac tcagagatat atctttggag
      241 gatgccatta aagcatccaa ttatgaagaa attaacaata aggtgactga taaaaaaatg
      301 gctcatcaag ctcttgctta ttctctaggc aatgcaaaat ctgatatggc actttattta
      361 ctatctaaat ttaattttac aaaacaagat atcgcagaga tggagaaaat gaacaacaat
      421 atgtattgtg agctgtatga tgttgagtat ttacttagtg aagatagtgc caactataaa
      481 gtgctagaat attttattag taatggattg gttgatgtaa acaaaa
//
LOCUS       DAJTRD010000324          515 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTRD010000324 DAJTRD010000000
VERSION     DAJTRD010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.331"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            55..>515
                     /locus_tag="OO315_004395"
     CDS             55..>515
                     /locus_tag="OO315_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5893015.1"
                     /translation="MTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINYMSKIKAI
                     RRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTNPV
                     AATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDL"
BASE COUNT          177 a          115 c          118 g          105 t
ORIGIN      
        1 actgaagcta tacaggccaa cattgagtta ttttcaggac acaaacacaa gcctctgaca
       61 gcgagaatca acagtgataa ttctgttacg ttacattcat ggcttgatcg ctacgaaaaa
      121 atcctcgcca gcagaggaat caagcagaag acactcataa attacatgag caaaattaaa
      181 gcaataagga gggggctgcc tgatgctcca cttgaagaca tcaccacaaa agaaattgcg
      241 gcaatgctca atggatacat agacgagggc aaggcggcat cagccaagtt aatcagatca
      301 acactgagcg atgcattccg agaggcaata gctgaaggcc atataacaac aaacccggtc
      361 gctgccactc gcgcagcaaa atcagaggta aggagatcaa gacttacggc tgacgaatac
      421 ctgaaaattt atcaagcagc agaatcatca ccatgttggc tcagacttgc aatggaactg
      481 gctgttgtta ccgggcaacg agttggtgat ttatg
//
LOCUS       DAJTRD010000325          511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTRD010000325 DAJTRD010000000
VERSION     DAJTRD010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.332"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..>511)
                     /locus_tag="OO315_004396"
     CDS             complement(<1..>511)
                     /locus_tag="OO315_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5893016.1"
                     /translation="AQCPGFLFAHREECTVEIKKIINPRYTESGAVDCDVFFDDRDQA
                     VPYTATADDVAPTGQQIWQELQSGKWGEIAPFTVTPEMLEAAREARRQEIEAWRAEQE
                     AKLFTFEWNGRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWSDADNQQVKLSMPELEE
                     LAAAMVQAQV"
BASE COUNT           97 a          152 c          126 g          136 t
ORIGIN      
        1 cgacctgcgc ctgcaccatt gctgccgcca gttcttccag ttccggcatc gacagtttca
       61 cctgctgatt atcggcatcg ctccacgcca tatgtgtttg tgctgtgaca gattttgcca
      121 gcatgactac cggggacagg cggcccagtg agtcgggacc agcattccag atacgaccgt
      181 tccattcaaa cgtgaacagc ttcgcctcct gttctgcgcg ccatgcttca atttcctgac
      241 gtctggcctc tctggccgct tccagcattt ctggtgtcac agtgaatggg gctatctcac
      301 cccatttgcc gctttgcagt tcctgccaga tttgctgacc cgtcggagcg acatcatcag
      361 cggtggctgt gtaggggact gcctggtccc tgtcgtcaaa aaaaacgtca cagtctactg
      421 cgccactttc ggtataacgg ggattaatga tttttttaat ttccacggtg cattcctcac
      481 gatgtgcgaa taaaaagccc gggcattgcg c
//
LOCUS       DAJTRD010000326          510 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTRD010000326 DAJTRD010000000
VERSION     DAJTRD010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.333"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            369..>510
                     /locus_tag="OO315_004397"
     CDS             369..>510
                     /locus_tag="OO315_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5893017.1"
                     /translation="MSQQPQQPQQEISIINLDQLVSMTSVEIAELTGKEHRNVLRDIR
                     KML"
BASE COUNT          123 a          137 c          112 g          138 t
ORIGIN      
        1 ctagggtctc tctataggtc ttctctatgg tctctctata ggtctctcta gggtctcctc
       61 taggcattac ccccagaagc taactctatg gccctctatg gtcctctatg gccctgtacg
      121 tcattcttta ggatgccctg tagtttcgta tagcctgatg cctagagaga ccctagagag
      181 accctagagt ggctcctaga gagaccctcc cagtagctcc gcacagctac atcgtcatca
      241 tcatagtatt caatctcaaa gtcattctgt ttgcatcctg ttgtcctgct ggtctccctt
      301 cgcttgggga ctttgagtag gtcagtgggg gtgcgacttc agggtgcaaa caaaaaggaa
      361 atcaagcgat gtctcaacaa ccacaacaac cacaacaaga aatcagcatc attaacctcg
      421 accagcttgt gtccatgacc tcagttgaaa ttgctgagct gacaggcaag gagcaccgaa
      481 atgtccttcg tgatattcgt aagatgctcg
//
LOCUS       DAJTRD010000327          508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTRD010000327 DAJTRD010000000
VERSION     DAJTRD010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.334"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..233
                     /locus_tag="OO315_004398"
     CDS             <1..233
                     /locus_tag="OO315_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5893018.1"
                     /translation="NGIKAVIPRKSNEKMASDGRAQLDRDAYCNRNVVERCFGRLKEY
                     RRIATRYDKTARNYLAMVKLGCIRLFYQRLRN"
     gene            348..>508
                     /locus_tag="OO315_004399"
     CDS             348..>508
                     /locus_tag="OO315_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5893019.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCAR"
BASE COUNT          148 a          131 c          127 g          102 t
ORIGIN      
        1 ataacggtat aaaggcagta atcccccgaa agtcaaatga gaaaatggca tcggatggac
       61 gtgcacagct tgatcgtgat gcgtactgca atcgtaatgt cgttgaacgg tgctttgggc
      121 ggctgaaaga atatcgccgc atcgccacgc gttacgacaa aacggcgaga aattacctgg
      181 cgatggtgaa actgggctgc atccgactct tttatcaacg cttacgtaat taagggacac
      241 agcctaaata gctgcgcgga atagtagatc cctttgaggg aacttagccc ggattgtgcg
      301 atctgatcaa tcgccaaatc aaaacaaatc accaaccgga ctgagcaatg ccgatcatag
      361 caccaatttc ccgtgacgaa cgacgcctga tgcagaaagc catccataaa acacacgata
      421 aaaattatgc ccgcagactg actgccatgc tgatgctgca ccggggcgac cgtgtcagcg
      481 acgttgccag aacgctctgc tgcgcccg
//
LOCUS       DAJTRD010000328          501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTRD010000328 DAJTRD010000000
VERSION     DAJTRD010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.336"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..385)
                     /locus_tag="OO315_004400"
     CDS             complement(<1..385)
                     /locus_tag="OO315_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5893020.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSSTFEQRRLVHRLILATVMACCG
                     FFLYPLKFSFIRPEVSGVTGWLFSQL"
     gene            complement(375..>501)
                     /locus_tag="OO315_004401"
     CDS             complement(375..>501)
                     /locus_tag="OO315_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HCR5893021.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          163 a          133 c          113 g           92 t
ORIGIN      
        1 caagttgcga aaatagccat cctgtcaccc cactcacttc aggacggata aaactaaact
       61 tcagcgggta gagaaaaaaa ccgcagcagg ccattaccgt tgccagaata agccggtgga
      121 caagtcggcg ttgttcgaat gtcgagctac aaacgaacag cgaaaatcca tataaaagat
      181 ccagactcca gtaaggaaca atagtccagg gaagaaaagg gatcgccgtt tcccaaccga
      241 atacctgact ggggatatca tggctgttaa ggtcctgaac cgcggtgaac tgattaagag
      301 atccataggt gaagaagaaa aacggggcca gcaacaataa ccagccagcg ccttgtagca
      361 atacgttccg acgttcagtt atcacgacgc accgccatcg aaaccgtaaa aatgccccac
      421 acatcaatcc gttgagtgca tttatcaaat ccggcattac gcacgagcga atccatctcc
      481 ccttgcgaac gcacgcgcat t
//
LOCUS       DAJTRD010000329          495 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTRD010000329 DAJTRD010000000
VERSION     DAJTRD010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.337"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(139..>495)
                     /locus_tag="OO315_004402"
     CDS             complement(139..>495)
                     /locus_tag="OO315_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5893022.1"
                     /translation="SICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQ
                     AVKAELFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTR
                     SEQKAAFSKQSIAKVD"
BASE COUNT          123 a          128 c           87 g          157 t
ORIGIN      
        1 actcatctga ccaccagcac gtttaacgtc ctgtgccatt tttcaggcat aaaaaaaccg
       61 ctcggcggcg ggttttgctt actttaccat cgcgtacaaa atcggcaaag tatcagattt
      121 gcatgagata tatgcctttt aatctacttt tgcaatactt tgctttgaaa atgccgcttt
      181 ttgttctgaa cgtgttctca tcacaaacaa taaagcctca ctatccagtc ggtgaaaaat
      241 gtgtttcatt acaacccagt gaccagtaaa tgttttggac cagtttttgg ttgttactcc
      301 cgccagtaat gccagctcct gatattcata accttcccca ccaaaaagtt ctgcttttac
      361 cgcctgcgcc gccagccaga ttaatttttt caggcgttcc tgtgttttct ctgcaatttt
      421 cctagtgcca gaatgagcct taaactcttc ccacgcccag tgggttatgg tgatctgata
      481 ctcccagcaa atact
//
LOCUS       DAJTRD010000330          493 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTRD010000330 DAJTRD010000000
VERSION     DAJTRD010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.338"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..224
                     /locus_tag="OO315_004403"
     CDS             <1..224
                     /locus_tag="OO315_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inhibitor of g-type lysozyme"
                     /protein_id="HCR5893023.1"
                     /translation="KVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLPAS
                     GKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(332..>493)
                     /locus_tag="OO315_004404"
     CDS             complement(332..>493)
                     /locus_tag="OO315_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5893024.1"
                     /translation="LQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVDGKRSGKINQTT
                     TKMLFMCRE"
BASE COUNT          159 a          116 c           89 g          129 t
ORIGIN      
        1 agaaagtgca tgtgagtatc tctaatgaag gcgctgatac ttatctcttc gggccaggaa
       61 tcgatgattc tgttgattta tccagatatt ccccagaact cgatagtcac ggacaatact
      121 cactccctgc ttctggcaaa tatgagctgc gagtgctgca aactcgaaac gacgcccgta
      181 aaaacaaaac taaaaaatat aacgtcgata ttcaaattaa gtaaatgtca accgacgggg
      241 actatccccg tcatagacgt taacgcccca ctcctctctg tcgctcaaga ggttaatgac
      301 gaatagcaga gaacataatg taaacaaggc atcactcacg acacataaat aacatctttg
      361 tggtggtttg attgattttt ccactccgct taccatctac accaaaataa cgatcatcac
      421 gacataattt ttctttaaca tcgcaaaata caccgttagc gaatgtaaat tcgctgggat
      481 cgtagccttg taa
//
LOCUS       DAJTRD010000331          483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTRD010000331 DAJTRD010000000
VERSION     DAJTRD010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.339"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..380
                     /locus_tag="OO315_004405"
     CDS             <1..380
                     /locus_tag="OO315_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5893025.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT          123 a          123 c          121 g          116 t
ORIGIN      
        1 tcggaatgaa ttatcaatta tatgtccgta attacagtcg taacaccaac tacgagatcg
       61 ttgctacggc tgatggcctg gatgtgctga acggcaagca aggttcgctg aataacaacg
      121 gctatattgt gaatgccgga gattcgttag tgattaaagg attccgcaaa gataaacata
      181 ctgaagcggc ttttcagttc gccaacgttg ctgattccta cgcagccaat tcggcgcaag
      241 gcgatgtgcg taataccggg gtaatcggct ttgccgcctt tgaattacaa ggcccggcgc
      301 aaaacgcgct gccgccgtgc tctggacaag cctttccggc agataacaat ggctatgcgc
      361 cgccaccgtg tcggaaatag caacctcgct tgatgcctcg cccatttaag gcgataatta
      421 gcacaaactt gtgtttcgca aatccaaccc ttcctcgtcg ggcctaacga cgcggaaggg
      481 ttt
//
LOCUS       DAJTRD010000332          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTRD010000332 DAJTRD010000000
VERSION     DAJTRD010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.341"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..>472
                     /locus_tag="OO315_004406"
     CDS             <1..>472
                     /locus_tag="OO315_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5893026.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAJTRD010000333          471 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTRD010000333 DAJTRD010000000
VERSION     DAJTRD010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.342"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(36..467)
                     /locus_tag="OO315_004407"
     CDS             complement(36..467)
                     /locus_tag="OO315_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5893027.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT          141 a          123 c           89 g          118 t
ORIGIN      
        1 gcagcatatt tagtatctga cacaaagcta tcattctaac ctttccgctc gacggaaatc
       61 atggtgacaa aaggatagcg ttgccacggg attgccccgc ctttcatata catatgtgaa
      121 atcgtgccac caagataaag cagctgctca acgttcagtt tcgctttggc ataacaggca
      181 aaatcataaa aatttgtcgc ctgctggctc aacaaaaaca cggcattccc atgtttatta
      241 atcccaacac cgttacgaat tttacgtgag gcgacgttgg gatgaatacg cggattaatt
      301 acaccgtttt ccatcaacat tggtcctgac tgcaccgcaa actgaatatc tttactggct
      361 ttaaaagcat ccagacgaac gatgccgact ttatctcccg cgacataaaa cacgccgcca
      421 ggacggataa agaaattccc ttcacctgaa gcgagattta acgccacctt c
//
LOCUS       DAJTRD010000334          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTRD010000334 DAJTRD010000000
VERSION     DAJTRD010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.343"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..>470)
                     /locus_tag="OO315_004408"
     CDS             complement(<1..>470)
                     /locus_tag="OO315_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HCR5893028.1"
                     /translation="HDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVI
                     EGAYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPR
                     RREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWV"
BASE COUNT           93 a          141 c          123 g          113 t
ORIGIN      
        1 acccacaatg cgcggttgag cttaatatcg gtgtcgatgt tgtgaatggc acgggtgtgg
       61 atacgttttc cttttgcact gcggccggaa atcccgcctt tcagcatatt ctcctggatg
      121 gtctgatatg cgctccacag gtctttaccg taatcctccc tgcgtcgtgg tgtcagaatg
      181 tcagcggtgg tgactggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc
      241 agcgcctggc gtgccggtgg cggcaggacc agcgactgca tggcatcacg cttttcctca
      301 atccggtcaa aaacccccac cacttcgtaa gccccttcga taactttctc caccacattt
      361 ccccggtgtg gaacacgcac ttcccccaga gactgaccgc agacgcagcc attctggcag
      421 acgaatctga agtaacccgg cagcatctgg tagctggagg taccgtcatg
//
LOCUS       DAJTRD010000335          470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTRD010000335 DAJTRD010000000
VERSION     DAJTRD010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.344"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..>470
                     /locus_tag="OO315_004409"
     CDS             <1..>470
                     /locus_tag="OO315_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HCR5893029.1"
                     /translation="HDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVI
                     EGAYEVVGVFDRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPR
                     RREDYGKDLWSTYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWV"
BASE COUNT          108 a          124 c          144 g           94 t
ORIGIN      
        1 catgacggta cctccagcta ccagatgctg ccgggttact tcagattcgt ctgccagaac
       61 gggtgtgtct gtggtcagtc tctgggggaa gtgcgtgttc cgcaccgggg aaacgtggtg
      121 gagaaagtta ttgaaggggc ttacgaagtg gtgggcgtgt ttgaccggat tgaggagaaa
      181 cgtgatgcca tgcagtcgct ggtcctgccg ccaccggcac gccaggcgct ggcacaggcg
      241 gcactgactt accgttatgg tgacgaacat cagcccgtca ccaccgccga cattctgacg
      301 ccacgacgcc gggaggatta cggtaaggac ctgtggagta cttatcagac catccaggag
      361 aatatgctga aaggcgggat ttccggccgc agtgcaaaag gaaaacgtat ccacacccgt
      421 gccattcaca acatcgacac cgatattaag ctcaaccgcg cattgtgggt
//
LOCUS       DAJTRD010000336          468 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTRD010000336 DAJTRD010000000
VERSION     DAJTRD010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 468)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 468)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..468
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.345"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
BASE COUNT          131 a           95 c           86 g          156 t
ORIGIN      
        1 tctgactcgc aattcatcca gcagtttatc atttcccata atgtcactta ccctcatcgg
       61 taaaaaaatt gcctttcgac cattacgata cattattgat tttggggttt cagggaagta
      121 atccatttcg actataactg acaggtcatc acgacgtatg actgcgtatt tgctactaaa
      181 tagtttcttt attttttcca cgatgccccc aggtttataa gtacaaacgg ttatatccac
      241 atagagacaa aaatattaat ctgataaata tttatttcac gccgtatatt tgattgttta
      301 atgtgcaagt acaatgactt ttattttttg ttgtgtatat aatcaaatat atggttattt
      361 ttcaccctgc gtattcagca cgcaacaaaa aacccgccga agcgggttta gtgcgggtgc
      421 gttgaggatg cctgacacat gagaggtggc gagggatttc tcccccgc
//
LOCUS       DAJTRD010000337          463 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTRD010000337 DAJTRD010000000
VERSION     DAJTRD010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.346"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..>463
                     /locus_tag="OO315_004410"
     CDS             <1..>463
                     /locus_tag="OO315_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5893030.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGC"
BASE COUNT          117 a          109 c          142 g           95 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcc
//
LOCUS       DAJTRD010000338          437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTRD010000338 DAJTRD010000000
VERSION     DAJTRD010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.347"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..437
                     /locus_tag="OO315_004411"
                     /pseudo
     CDS             1..437
                     /locus_tag="OO315_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          126 a          114 c          100 g           97 t
ORIGIN      
        1 acgacaatgt gttacgtgcc atcgcaaata ctgaatgctc acctgaattt aacgccctca
       61 attttgagga cgtcacctac accgacacca aaggcgaaaa acgcccaatg taccaaatca
      121 ccaaaaacgg cttcgttttc ctggtgatgg gcttcactgg gaaaaaagcc gctgcattca
      181 aagaggccta catcgctgat gttcgaccgc atggaggcag aactgcacca gaataatacc
      241 ccgtccgctg acaagatgat tccgggtaac ggtcgcactc tggttgtgca cttcgacgaa
      301 cacggcaata tcagattcac cgaaactgtt cctgacggtg cactggtctg taccctggat
      361 actttccagt tttatctgga aaaacagggg tgggctcttg taaaccggag cgcaattaaa
      421 aatatgaccg tagagca
//
LOCUS       DAJTRD010000339          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTRD010000339 DAJTRD010000000
VERSION     DAJTRD010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.348"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            35..434
                     /locus_tag="OO315_004412"
                     /pseudo
     CDS             35..434
                     /locus_tag="OO315_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          100 a          112 c          114 g          108 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacattggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggac
//
LOCUS       DAJTRD010000340          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTRD010000340 DAJTRD010000000
VERSION     DAJTRD010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.349"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..149
                     /locus_tag="OO315_004413"
                     /pseudo
     CDS             1..149
                     /locus_tag="OO315_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(169..>431)
                     /locus_tag="OO315_004414"
     CDS             complement(169..>431)
                     /locus_tag="OO315_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HCR5893031.1"
                     /translation="STGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANSIKSRR
                     VISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          110 a          104 c          101 g          116 t
ORIGIN      
        1 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
       61 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      121 cccgactgtg tcgccccttc aggctgaggg atccccacaa aattactgct aataaaccag
      181 caccatattt cggtaggttc tggacagatg gtttagaact gtgctcagta ctgttcgtct
      241 taaaataagt ggcgagtgtc gaagaacatt cttcgctaac gtcagatatg agataacccg
      301 ccgggattta atgctgttag cctgatattt caggtgtaac cctttatttt cagcgtgata
      361 accgagtaac cacattacga tagtgctcaa cgttgccagc agactcagaa ccagaattct
      421 tcctgttgaa c
//
LOCUS       DAJTRD010000341          427 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTRD010000341 DAJTRD010000000
VERSION     DAJTRD010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.350"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..192)
                     /locus_tag="OO315_004415"
     CDS             complement(<1..192)
                     /locus_tag="OO315_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR5893032.1"
                     /translation="MQSERIYLVWAHPRHDSLTANIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDK"
     gene            292..>427
                     /locus_tag="OO315_004416"
     CDS             292..>427
                     /locus_tag="OO315_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5893033.1"
                     /translation="MRMNMSDFATSFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     L"
BASE COUNT          109 a          103 c           89 g          126 t
ORIGIN      
        1 tttatccata ttcttccagt ctggttcatc ttccgacgtc atcaccgggt tgaaattacg
       61 ccgatataaa tcgagttccg tcacctgtat tttccgctcc attgcccgct gatggatcgc
      121 atcagcaata tttgcggtca atgaatcatg acgaggatgg gcccatacca aataaatacg
      181 ttcagactgc ataattttac ctccacgtta agttctggag cgcactttac gcaggcggca
      241 taatcctgta aacgtgatga ttcaggatga gttaatgaag gattttcact aatgcggatg
      301 aatatgtctg actttgccac ttcctttgcc gtggcccgta atcaaagctt tcgtgcagca
      361 ggcgatgagt taggcttatc ctcgtccgcc attagccata gtattaaaac actggaacaa
      421 cgtctta
//
LOCUS       DAJTRD010000342          423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTRD010000342 DAJTRD010000000
VERSION     DAJTRD010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.351"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..423
                     /locus_tag="OO315_004417"
                     /pseudo
     CDS             1..423
                     /locus_tag="OO315_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          111 a          111 c          116 g           85 t
ORIGIN      
        1 attgaaatgg aaaatgaaat attaaaaagg ctaccgcgct cttgatgtca gactccctga
       61 acagttttcg ataatcggga aactcagggc gcgttatcct gtggccactc tctgccatgt
      121 gttcggggtc catcgcagca gctacaaata ctggaaaaac cgtcctgaaa aaccagacgg
      181 cagacgggct gtattacgca gccaggtact tgaactgcat ggcatcagcc acggctctgc
      241 cggagcaaga agcatcgcca caatggcaac ccagagaggc taccagatgg ggcgctggct
      301 tgctggcaga ctcatgaaag agctggggct ggtcagttgc cagcagccga ctcaccggta
      361 taagcgtggc ggtcatgagc acgttgctat cccgaatcat cttgagcgac agttcgccgt
      421 aac
//
LOCUS       DAJTRD010000343          419 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTRD010000343 DAJTRD010000000
VERSION     DAJTRD010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..419
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.352"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            88..153
                     /locus_tag="OO315_004418"
                     /pseudo
     CDS             88..153
                     /locus_tag="OO315_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT          132 a           87 c           94 g          106 t
ORIGIN      
        1 atagatgcac aaaaaacggg caaaactacc tggttcgcaa aactgcgtct aaagttaaac
       61 tgggacctcg cgagcaaggg tgagacgatg gcgctttaca caattggtga agtggcgttg
      121 ctttgtgata ttaatcctgt cacgttacgc gcgtgacgca acatttttaa aaatcgacga
      181 agatcgtcca ggagcgccgt ttttcaaggg ttggatagat gattcgttta ccatgtcgac
      241 gaacttcgac gaatttcgcc gataaaaagt gtcccataca tgccccattg atgccccata
      301 aacaatatca ggaattgaca ctggttatcc atacagataa aaataacacc tgtacataac
      361 tacagttaca ggagggtgtt atgcgtgttg agatctgtat cgctaaagaa aaaatcact
//
LOCUS       DAJTRD010000344          412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTRD010000344 DAJTRD010000000
VERSION     DAJTRD010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.353"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..>412
                     /locus_tag="OO315_004419"
     CDS             <1..>412
                     /locus_tag="OO315_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR5893034.1"
                     /translation="VGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNV
                     PHKDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFN
                     FLASHDGIGLNPLRGLLPESEILALVEALQQEGA"
BASE COUNT          107 a          100 c          108 g           97 t
ORIGIN      
        1 ccgttggctt tatgtggaaa gagccgggaa caagctgcat ccatctggaa aaaacacatc
       61 tgattatcaa gctgttacgg tcgattattg atgacgtagc accaggtaca gtgattatta
      121 ccgaaaccaa tgttccgcac aaagacaaca ttgcttactt tggcaacggt gatgacgaag
      181 cgcatatggt gtaccagttc tcgctgccgc cgctggtgtt acatgcggtg caaaaacaga
      241 acgtcgaggc gctgtgccag tgggcgcaaa gtctgagctt gccctccagt aaaacgacct
      301 ggtttaactt cctcgcctct cacgatggca tcgggttgaa cccactgcgt ggcttgcttc
      361 cagaaagcga aatattagcg ctggtcgagg cattacagca ggaaggtgca tt
//
LOCUS       DAJTRD010000345          410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTRD010000345 DAJTRD010000000
VERSION     DAJTRD010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.354"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            156..341
                     /locus_tag="OO315_004420"
                     /pseudo
     CDS             156..341
                     /locus_tag="OO315_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          119 a           97 c          108 g           86 t
ORIGIN      
        1 atacggtggt cggtttacgc ttaccgcagg acaggtggta actgaaaaat agtttgctga
       61 ggcgactgga aacagaaggg caagatcgat tccgacagca gtgatggtca gctacctcat
      121 cgataacttt gcaacagtgc ccttcttgaa gcatcatgct tcatgaagaa aaactggcac
      181 gtcatcaacg taaacaggcg atgtataccc gaatggcagc cttcccggcg gtgaaaacgt
      241 tcgaagagta tgacttcacg ttcgccaccg gagcaccgca gaagcagctc cagtcgttac
      301 gctcactcag cttcatagag cgcaatgaaa acatagtttt acaggggaca tctgacatca
      361 caaatccccg ggtggggatt tgtgtataag agacagccca tggaggaagg
//
LOCUS       DAJTRD010000346          408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTRD010000346 DAJTRD010000000
VERSION     DAJTRD010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.355"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(212..>408)
                     /locus_tag="OO315_004421"
     CDS             complement(212..>408)
                     /locus_tag="OO315_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5893035.1"
                     /translation="THMAWSDADNQQVKLSMPELEELAAAMVQAQVDRNDEIYRRQRE
                     MKEELSGLDDLASIRAFDVE"
BASE COUNT           89 a          140 c           84 g           95 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaccac agaccggact
       61 gactccggaa aaacggaggc ccgcccccgg gcctcccctg attcatccgt ttccctgttc
      121 agcctgacag cacgccccgg ctgccggatg acagactccg cttcggtaag caaagcggtc
      181 ttctgtgatt ccgccagttg cggcttattc attactcaac gtcaaacgcc cgaattgaag
      241 ccaaatcatc cagaccgctc agctcctctt tcatttcacg ctgacggcga taaatctcat
      301 cgttgcgatc gacctgcgcc tgcaccattg ctgccgccag ttcttccagt tccggcatcg
      361 acagtttcac ctgctgatta tcggcatcgc tccacgccat atgtgttt
//
LOCUS       DAJTRD010000347          405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTRD010000347 DAJTRD010000000
VERSION     DAJTRD010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.356"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(45..>405)
                     /locus_tag="OO315_004422"
     CDS             complement(45..>405)
                     /locus_tag="OO315_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5893036.1"
                     /translation="DHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVC
                     GEKLPQVYRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVE
                     GLKKNARDISLLRYMPA"
BASE COUNT           75 a          139 c           88 g          103 t
ORIGIN      
        1 tgttttttat acttgcaccg attcccgggc acacttagcc tgtgtcaggc cggcatatac
       61 ctcagcagac tgatatcccg cgcgtttttc ttcagcccct ccacattcag tcccgcgatg
      121 gcccggcggt ataccatccg gccgccacac agcacgcatt cgaacgggtc acgactcagg
      181 aactgtttca ccatttgtgc atagcacact ttcgccactg gttccggttt atccatcccc
      241 agagcacggt acacctgcgg cagcttctct ccacacaccc ggttggcaag gaacccgaag
      301 tacctcacca tcttaaaaaa cttctccggg atgtgctgtt tcagcctcgc gaccagctca
      361 cgctgtgtca gcgtttccgt cgccgtttct cccgttttgt ggtcc
//
LOCUS       DAJTRD010000348          400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTRD010000348 DAJTRD010000000
VERSION     DAJTRD010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.357"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..322)
                     /locus_tag="OO315_004423"
     CDS             complement(<1..322)
                     /locus_tag="OO315_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5893037.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGG"
BASE COUNT           96 a          122 c           88 g           94 t
ORIGIN      
        1 gaccaccagg aacaggcagt ccgcgcacaa tattggtttc atcaaactgc tcgtcgtcga
       61 taagcaaccc cgcatgtgtc agtgcatcca gtggtgcttt caggatattg tccaggtcac
      121 gacgccgctt atccggtggc tctgcaataa tctttatcgc cagtcttccg gacaggctta
      181 atttcagtcg ctgctgacgg acaataagcg ccacatcccg gcgataacgc tcaccggctt
      241 ttgatacaaa atatgtgctg ccacgacgtc gccagtaagt attcaccgtc ggcggataag
      301 gtaaaataaa ctcatggcgc atcagcgcag cacctcctgc accagctttt caaactttcc
      361 gactctggtt tccagctctg ccacacagtc caccagctca
//
LOCUS       DAJTRD010000349          395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTRD010000349 DAJTRD010000000
VERSION     DAJTRD010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.358"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
BASE COUNT           89 a          120 c          102 g           84 t
ORIGIN      
        1 atttttaatc ctatctgagt gtttagcatt gttgcgctcc tttcatgtgc aatgccatat
       61 aaagccactg gcggagggag cgcgcagggg gcagccagtc gccgccgccc cctgcacccc
      121 cgggctctgg cgaacaaaat cgccgctgcg cggtaccttc agcttatccc cttcggcaac
      181 cgggtcgggc gcgaggtaac gtcctgtaag acgcgccctc agcccacatc catgtgggct
      241 gccccggcct tcggggaacg cttcagcgat tttgacgcca ccaatacccg tgcggtttgt
      301 aatgtcgaag ataaaacaac cagggcagat aaaatgccaa tgctaattaa tcacgctttt
      361 ccagcagagt ccggtaaatc agaccaccga taatg
//
LOCUS       DAJTRD010000350          388 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTRD010000350 DAJTRD010000000
VERSION     DAJTRD010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.359"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            28..388
                     /locus_tag="OO315_004424"
                     /pseudo
     CDS             28..388
                     /locus_tag="OO315_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
BASE COUNT          116 a           84 c          104 g           84 t
ORIGIN      
        1 aacaaatggt agtgtccgct attgccagta cacctcaaag agagtaaatc aaaccgcgca
       61 attggcgttg cgctgaatga tactgcatgt cgcgtattga aaaaacaaat cggaaatcat
      121 caccgttggg tatttgtgta caaggaaagc tgtaccaaac cagacggaac gaaagcgcca
      181 acagtaagga agatgcggta tgacgcaaac acagcctgga aagcggcgct gagacgggct
      241 ggtattgatg atttcagatt tcacgacttg agacacacct gggcaagttg gctggttcaa
      301 gccggagtcc cgttatcagt gttacaggaa atgggaggct gggagtctat cgaaatggtt
      361 cgtcgatatg ctcacctcgc acctaatc
//
LOCUS       DAJTRD010000351          375 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTRD010000351 DAJTRD010000000
VERSION     DAJTRD010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.360"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            260..318
                     /locus_tag="OO315_004425"
                     /pseudo
     CDS             260..318
                     /locus_tag="OO315_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           99 a           83 c          105 g           88 t
ORIGIN      
        1 aaaaaaaatc agcgtcaaga aggtttcatt aacacatatc cctggcccaa aaagccgggg
       61 attttttata tctgcgttcc gctaaaaggt gcaaatgctc aggacgttgc agcgttttgc
      121 gtgaccgctc ggggaaggca aaactgcttc agggaaagcg ttgcgcgggg tccggcgctc
      181 atcaacaatc ggggagtcag caaggggctg aaacgggaaa gcccctcccg aggaaggggc
      241 cttatagaag gaaagggtta tgatgaagtt cgtcatcata ctggttggtt gttactcgta
      301 agtttcgcag cttattaaca gccaatcaga ggggggagaa atcctccctc acccttgtgc
      361 ctttacttta ggttg
//
LOCUS       DAJTRD010000352          373 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTRD010000352 DAJTRD010000000
VERSION     DAJTRD010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..373
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.361"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..>373
                     /locus_tag="OO315_004426"
     CDS             <1..>373
                     /locus_tag="OO315_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5893038.1"
                     /translation="RILTDRENGEKGDNSQDTSYARVGVKGETQINPEMTGYGQFELD
                     LEASNRHNPDQTRLAYAGLSYKDFGSFDYSRNVGVAYDAEAFTDMFVEWGGDSWAGTD
                     LFMTNRTNGVATYRNTDFFGMV"
BASE COUNT          101 a           78 c           93 g          101 t
ORIGIN      
        1 agcgcattct gaccgaccgt gaaaatggtg aaaaaggcga taattctcaa gatacctctt
       61 atgcccgtgt aggcgtgaaa ggtgaaactc agatcaaccc tgaaatgact ggttacggtc
      121 agtttgaact agacctggaa gcatcaaaca gacacaaccc tgaccaaact cgtttggcat
      181 atgctggttt gagttacaaa gattttggtt cattcgacta cagtcgtaat gtcggtgtcg
      241 cgtatgatgc agaagcattt actgatatgt ttgttgagtg gggcggtgac tcatgggctg
      301 gtactgacct gttcatgacc aaccgtacca acggtgttgc aacctatcgt aacacagatt
      361 tctttggcat ggt
//
LOCUS       DAJTRD010000353          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTRD010000353 DAJTRD010000000
VERSION     DAJTRD010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.362"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..311)
                     /locus_tag="OO315_004427"
     CDS             complement(<1..311)
                     /locus_tag="OO315_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5893039.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT           99 a           74 c           96 g          103 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa ga
//
LOCUS       DAJTRD010000354          363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTRD010000354 DAJTRD010000000
VERSION     DAJTRD010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.363"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..348
                     /locus_tag="OO315_004428"
     CDS             <1..348
                     /locus_tag="OO315_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5893040.1"
                     /translation="IAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALE
                     GCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETS
                     NIQLADSVMTPTY"
BASE COUNT           97 a           86 c           82 g           98 t
ORIGIN      
        1 attgctccag cttgtaccct ggtagctgcg acgaaagatt cagtagtcac attgccaaat
       61 gtcagcgcca ccaagttgca aactaatgga gcagtctctg gcgttaaaac tgatgtacca
      121 attgccttag aaggctgtga tgttactgta actaaaaatg cgacgtttac ctttagtggt
      181 acggctgacg gcgtgcaacc tacagcattt gccaaccagg ctaccaccga tgcagcgacg
      241 aatgttgctc tacaaatgta tctgccagat ggttctacat cggtaactcc cggtaccgaa
      301 acgtcgaata ttcagttagc ggattcggta atgactccaa cttactgata gtgttttatg
      361 ttc
//
LOCUS       DAJTRD010000355          360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTRD010000355 DAJTRD010000000
VERSION     DAJTRD010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.365"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(117..311)
                     /locus_tag="OO315_004429"
     CDS             complement(117..311)
                     /locus_tag="OO315_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5893041.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
BASE COUNT           98 a           91 c           65 g          106 t
ORIGIN      
        1 ataaaacatt caagataggc gcgccgtgta ttttaattct tttaagactc ttcaaaaatg
       61 tcatcgtagc ctttaaaaac gaattttaaa ggcaccctaa aggcaggtca atgaagttaa
      121 cccgccttcg cgtttacact ctctgagaga atactagctt caatctcttc caaaatctct
      181 ctgacttgct cgtcgggcat atcaacgagc atcaacgctg ccaggcgttt acggccctct
      241 agttgcagta gctcttgagt ttccttatct aacaagcgta gcacagtctc aacacctttc
      301 cttgcgagca tgtttgtaag ggcttctcgt tcctcactgg taagaaacga caacatttct
//
LOCUS       DAJTRD010000356          359 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTRD010000356 DAJTRD010000000
VERSION     DAJTRD010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..359
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.366"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
BASE COUNT           76 a          102 c           90 g           91 t
ORIGIN      
        1 agcctgcgct gatgcagcat gtccctgaag gcgtgattca ggcaccagaa tcacccgacg
       61 ccccggaggc gctgccgggg tgtcgttcca tttctgatga tgacctgtgc gcctggtgta
      121 cccgtctgct ttaccgtccc ggagaaatca gtctgtgcag actgagtata caggcaatgg
      181 catctggcca tccgtctgtg accggaacgg ttacgcgtac ggctgtccgg aatttcagcc
      241 gaatatcatt catccctgat attaccgggt catcacggtc agtgatgaac gtgatgttgc
      301 cagctcccga cctttactcc ctctcattta ttcacattac aaggatttca tacatgaaa
//
LOCUS       DAJTRD010000357          355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTRD010000357 DAJTRD010000000
VERSION     DAJTRD010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.367"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..>355
                     /gene="tuf"
                     /locus_tag="OO315_004430"
     CDS             <1..>355
                     /gene="tuf"
                     /locus_tag="OO315_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5893042.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGF"
BASE COUNT           77 a           92 c          109 g           77 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcc
//
LOCUS       DAJTRD010000358          355 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTRD010000358 DAJTRD010000000
VERSION     DAJTRD010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..355
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.368"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..>355)
                     /locus_tag="OO315_004431"
     CDS             complement(<1..>355)
                     /locus_tag="OO315_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5893043.1"
                     /translation="LSSAAVKTAGVLISFQAVLSAYQRVMEVGLQRASAERSIDFVFS
                     KDANQVKDFTKSLAQTTGLDIAELQSQFAGFGASARESLGIQGSEELFRNMIGYSRLM
                     GRSEEEIKRALTALS"
BASE COUNT          104 a           80 c           64 g          107 t
ORIGIN      
        1 tgtgataatg ctgtcagtgc tcgcttaatc tcttcttcac ttcgccccat cagacgagaa
       61 tagccaatca tatttctaaa aagttcttca gaaccttgaa tgcctagaga ctctctagct
      121 gaagcaccga aaccagcaaa ctgtgattgc agttcagcga tatctaaacc cgttgtttga
      181 gctaatgact ttgtgaaatc ttttacttga tttgcatctt tagaaaatac gaaatcaatt
      241 gaacgttcag cacttgcacg ttgaaggcca acttccatta ctcgctgata agcggatagt
      301 acagcctgaa agctgataag tacacctgct gttttaactg ctgctgaact taaat
//
LOCUS       DAJTRD010000359          352 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTRD010000359 DAJTRD010000000
VERSION     DAJTRD010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..352
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.369"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
BASE COUNT          152 a           50 c           53 g           97 t
ORIGIN      
        1 taattttacg cctactacca tacaagagag aaaaagcgat tattcaaccg tgatcacatt
       61 aaaaacaaaa atttctaccg aggagaaaaa ataccctcaa aaaccacctg aataattaac
      121 cattattgtt aaaacaacac gttgaataat cacataacaa actccattaa tcattgaaaa
      181 tgagcacaga aatagaaatt aattatattc aattaattaa aatgtgtatt gcttgatgta
      241 tttaaaaata ttattttcat ggtgagagtg gaggtaaaga agacacaata ataaaaagga
      301 atgtgacaaa taatggttct ggttgggcgt taaatttaag gtcaccagaa tg
//
LOCUS       DAJTRD010000360          342 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTRD010000360 DAJTRD010000000
VERSION     DAJTRD010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..342
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.370"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            1..200
                     /locus_tag="OO315_004432"
                     /pseudo
     CDS             1..200
                     /locus_tag="OO315_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415867.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          131 a           62 c           66 g           83 t
ORIGIN      
        1 attggctacg taattaaaac agaaaataaa gaaaccccat tcactgtagt tgatatcgac
       61 ggtctatcag gaaactttaa aactcttgtt gaaggtgcca caaaaatgtg cctagtttac
      121 atcggaaata atctgcccgc agaaaaaaag ccgaatttct ggcaaatctg attgcaatga
      181 aattaaaagg tgaaatctga aaaaaagaaa gcctgcacag tgtgcaggcc tgagtgaaga
      241 acctgggaca tttattcatc actcgcagta attttaatct gagttgaggt taaaaaacaa
      301 tgagcacaaa accactcttc ctgttacgga aagcgaaaaa at
//
LOCUS       DAJTRD010000361          335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTRD010000361 DAJTRD010000000
VERSION     DAJTRD010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.371"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
BASE COUNT           98 a           88 c           65 g           84 t
ORIGIN      
        1 aataaggact aacatgactt ttaactgtgc cgcgtgcgca tagccgcatt caccgcatca
       61 caaaattcac tttaaaaagg gcggacatca gccagcaatt aaaccgatgc cgccaactgg
      121 tacttcacac agcaatgtcg ttatttacaa ccggaagcgc actcccacca tttaaatttc
      181 acagacaaga ccgactcttt atggataccg gaaatgcgcc ttcgtgttgt gcccggtttt
      241 atttcaccac ttccgggctt tggtggtatc tttactgaag ttctcacaca accagtaagg
      301 aaatgaatat gccaacgtat ctcgccagag tagaa
//
LOCUS       DAJTRD010000362          320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTRD010000362 DAJTRD010000000
VERSION     DAJTRD010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.372"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..75)
                     /locus_tag="OO315_004433"
     CDS             complement(<1..75)
                     /locus_tag="OO315_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HCR5893044.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFL"
BASE COUNT           93 a           81 c           61 g           85 t
ORIGIN      
        1 caaaaaccag tagccggcac tgccggcgga ggtgccgtag gcaatgcctg ttgatatttt
       61 ttccatctga tacatatccc gcccccccga cagacctgtg ctatcggaaa gaaaaaaggc
      121 catcagcaaa actctgatgg cctgaatcac ctttaccaat attgtatgaa aaaacacgca
      181 cgactaattg acaataattt tcatttccat taaaaaagat cgcgtagcat tcttaattca
      241 tgaggaactt tacccgccag caatctgagt agcgaaagct gttcatcccc aatgtttttg
      301 ctggcgggtc cttttttcta
//
LOCUS       DAJTRD010000363          316 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTRD010000363 DAJTRD010000000
VERSION     DAJTRD010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.373"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..315)
                     /locus_tag="OO315_004434"
     CDS             complement(<1..315)
                     /locus_tag="OO315_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001345277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5893045.1"
                     /translation="MYIVTAFALNYSTQNMGLPRELFLNIGLLVGGLSCLTIPCFAWL
                     ADRFGRRRVYITGALIGTLSAFPFFMALEAQSIFWIVFFSIMLANIAHDMVVCVQQPM
                     FTE"
BASE COUNT          108 a           76 c           67 g           65 t
ORIGIN      
        1 ttcggtaaac atcggttgtt gcacacacac caccatgtca tgcgcaatgt ttgccagcat
       61 tatggagaag aaaactatcc agaaaataga ttgtgcttca agcgccataa agaaaggaaa
      121 tgcgctcaac gttccgatca acgcgcctgt gatataaacc ctgcgccgac caaaacgatc
      181 ggcaagccag gcaaaacagg gaattgtcag gcagcttaat ccacctacca gcaaaccaat
      241 attaaggaaa agttcgcgcg gtaaccccat attctgggtt gaataattaa gtgcaaaggc
      301 agtaacaatg tacatc
//
LOCUS       DAJTRD010000364          313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTRD010000364 DAJTRD010000000
VERSION     DAJTRD010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.375"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..>313)
                     /locus_tag="OO315_004435"
     CDS             complement(<1..>313)
                     /locus_tag="OO315_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5893046.1"
                     /translation="RLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTP
                     TGTLKINAARVAARIFLMPLLVGFTREYPDIKVELTTDDSLIDIVQQGFDAGVRLSGI
                     V"
BASE COUNT           90 a           70 c           72 g           81 t
ORIGIN      
        1 tcgacgatgc cactaagtcg aacgccagcg tcaaagccct gttggacgat atcgataaga
       61 gaatcgtcag ttgtgagctc tactttaata tcaggatatt cacgtgtgaa accaaccagt
      121 aaaggcatta aaaatatacg tgcagctacc cgggcggcat tgattttgag cgttcctgtc
      181 ggtgtcagac gaaaatcatt catctcatct aacatgatct gaatttcgtc gaaagctggg
      241 cgcaatcgtt catagagatt agaacccgct tccgttaacg atacgcttcg ggttgtccga
      301 ttgaataagc gaa
//
LOCUS       DAJTRD010000365          305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTRD010000365 DAJTRD010000000
VERSION     DAJTRD010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.376"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            17..>305
                     /locus_tag="OO315_004436"
     CDS             17..>305
                     /locus_tag="OO315_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HCR5893047.1"
                     /translation="MSPSLRKAVAVAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLVTVARQINPYIKVDIPE"
BASE COUNT           90 a           66 c           76 g           73 t
ORIGIN      
        1 ctgcgagagg tgaataatgt cgccatcatt acgcaaggct gttgcagttg ctattggtgg
       61 cggggctgtt gctatagcat ctgtgttaat cactggccca ggtggtaacg atggtctgga
      121 aggtgtcagc tacataccat acaaagatat cattggtgta tggactgtat gtcacggaca
      181 caccggaaaa gacatcatgc ctggtaaaac gtataccgaa gcagaatgca aagctctcct
      241 gaataaagac cttgtcactg tcgccagaca aattaacccg tacatcaaag tcgatatacc
      301 ggaaa
//
LOCUS       DAJTRD010000366          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTRD010000366 DAJTRD010000000
VERSION     DAJTRD010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.377"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            92..>304
                     /locus_tag="OO315_004437"
     CDS             92..>304
                     /locus_tag="OO315_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5893048.1"
                     /translation="MIYSNQLRYTGKDGVYFHLQGQPDNFLSLSATENMEFDSPMMVT
                     TQNMQSGQTVTDNVMRSPRTISISGVV"
BASE COUNT          106 a           55 c           53 g           90 t
ORIGIN      
        1 ctcacgctta acagagaaaa tacttatata tcaaaggctt ggttaaaaat gattaaaatg
       61 cgctaagtct ttgaaatcaa aagagaattt aatgatttac tctaatcagc ttcgttacac
      121 tggcaaagat ggtgtttact tccatctaca aggccaacca gataatttct tgtcactatc
      181 agcaactgaa aacatggaat ttgatagccc tatgatggtt accactcaaa atatgcaatc
      241 agggcagact gtaacagata atgtaatgcg ttcacctaga actatttcaa tcagtggtgt
      301 ggtt
//
LOCUS       DAJTRD010000367          295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTRD010000367 DAJTRD010000000
VERSION     DAJTRD010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.378"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            <1..220
                     /locus_tag="OO315_004438"
     CDS             <1..220
                     /locus_tag="OO315_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5893049.1"
                     /translation="LYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHW
                     GFSDQSHFSTVFKQRFGMTPGEYRRKFR"
BASE COUNT           76 a           58 c           72 g           89 t
ORIGIN      
        1 tttgtaccga atgtttgccg ataaaggttt ggtagtcgcg caatatattc gtaaccgtcg
       61 tctcgatttt tgtgcagatg cgattcgcca tgccgcagat gatgaaaaac tggcaggcat
      121 cggctttcat tggggatttt ctgaccagag tcatttttca acggtattta agcaacgctt
      181 tgggatgacg ccaggcgagt atcgacgtaa attccgctaa ctaattggcg ttgcatgtac
      241 tgcaacgcca attagttaaa acagatattt gactatgcat ttttcagggc gaaag
//
LOCUS       DAJTRD010000368          294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTRD010000368 DAJTRD010000000
VERSION     DAJTRD010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.denovo.379"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..>294)
                     /locus_tag="OO315_004439"
     rRNA            complement(<1..>294)
                     /locus_tag="OO315_004439"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           53 a          102 c           64 g           75 t
ORIGIN      
        1 aatcctgttt gctccccacg ctttcgcacc tgagcgtcag tcttcgtcca gggggccgcc
       61 ttcgccaccg gtattcctcc agatctctac gcatttcacc gctacacctg gaattctacc
      121 cccctctacg agactcaagc ttgccagtat cagatgcagt tcccaggttg agcccgggga
      181 tttcacatct gacttaacaa accgcctgcg tgcgctttac gcccagtaat tccgattaac
      241 gcttgcaccc tccgtattac cgcggctgct ggcacggagt tagccggtgc ttct
//
LOCUS       DAJTRD010000369         5818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.guided.170, whole genome shotgun sequence.
ACCESSION   DAJTRD010000369 DAJTRD010000000
VERSION     DAJTRD010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.guided.170"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO315_004440"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO315_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5893050.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2123
                     /locus_tag="OO315_004441"
                     /pseudo
     CDS             1596..2123
                     /locus_tag="OO315_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            2144..2617
                     /locus_tag="OO315_004442"
     CDS             2144..2617
                     /locus_tag="OO315_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR5893051.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2692..2967
                     /locus_tag="OO315_004443"
                     /pseudo
     CDS             2692..2967
                     /locus_tag="OO315_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            2967..3803
                     /locus_tag="OO315_004444"
     CDS             2967..3803
                     /locus_tag="OO315_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5893052.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3910..4314)
                     /locus_tag="OO315_004445"
                     /pseudo
     CDS             complement(3910..4314)
                     /locus_tag="OO315_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4358..4747)
                     /locus_tag="OO315_004446"
     CDS             complement(4358..4747)
                     /locus_tag="OO315_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5893053.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4965..5405
                     /locus_tag="OO315_004447"
     CDS             4965..5405
                     /locus_tag="OO315_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5893054.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1399 a         1539 c         1517 g         1363 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggttttttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgttaacc caggataaga accttgaaag tatcattgat
     2161 agctgcgaaa gcgaaaaacg gcgtgattgg ttgcggtcca gacataccct ggtccgcgaa
     2221 aggggagcag ctacttttta aagcattgac ctacaatcag tggcttctgg tgggtcgcaa
     2281 gacgtttgaa tctatgggcg cactccccaa taggaaatac gcggtcgtta cccgctcagg
     2341 ttggacatca aatgatgaca atgtagttgt atttcagtca atcgaagagg ccatggacag
     2401 gctagctgaa ttcaccggtc acgttatagt gtctggtggc ggagaaattt accgagaaac
     2461 attacccatg gcctctacgc tccacttatc gacgatcgac atcgagccag agggggatgt
     2521 tttcttcccg agtattccaa ataccttcga agttgttttt gagcaacact ttacttcaaa
     2581 cattaactat tgctatcaaa tttggaaaaa gggttaacaa agctatgcaa tcgacggcaa
     2641 aaagcttcgt tcgcttcgcg cactacgcct ttttccgcga ttgatagcga cgatatggtt
     2701 gtttgccatg gtgatccctg catgccgaac ttcatggtgg accctaaaac tcttcaatgc
     2761 acgggtctga tcgaccttgg gcggctcgga acagcagatc gctatgccga tttggcactc
     2821 atgattgcta acgccgaaga gaactgggca gcgccagatg aagcagagcg cgccttcgct
     2881 gtcctattca atgtattggg gatcgaagcc cccgaccgcg aacgccttgc cttctatctg
     2941 cgattggacc ctctgacttg gggttgatgt tcatgccgcc tgtttttcct gctcattggc
     3001 acgtttcgca acctgttctc attgcggaca ccttttccag cctcgtttgg aaagtttcat
     3061 tgccagacgg gactcctgca atcgtcaagg gattgaaacc tatagaagac attgctgatg
     3121 aactgcgcgg ggccgactat ctggtatggc gcaatgggag gggagcagtc cggttgctcg
     3181 gtcgtgagaa caatctgatg ttgctcgaat atgccgggga gcgaatgctc tctcacatcg
     3241 ttgccgagca cggcgactac caggcgaccg aaattgcagc ggaactaatg gcgaagctgt
     3301 atgccgcatc tgaggaaccc ctgccttctg cccttctccc gatccgggat cgctttgcag
     3361 ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg tcaaactgac tacgtccacg
     3421 cggcgattat agccgatcaa atgatgagca atgcctcgga actgcgtggg ctacatggcg
     3481 atctgcatca tgaaaacatc atgttctcca gtcgcggctg gctggtgata gatcccgtcg
     3541 gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt ctacgatccg gctgacagag
     3601 acgacctttg tctcgatcct agacgcattg cacagatggc ggacgcattc tctcgtgcgc
     3661 tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc ttatgggtgc ctttccgcag
     3721 cttggaacgc ggatggagaa gaggagcaac gcgatctagc tatcgcggcc gcgatcaagc
     3781 aggtgcgaca gacgtcatac tagatatcaa gcgacttctc ctatcccctg ggaacacatc
     3841 aatctcaccg gagaatatcg ctggccaaag ccttagcgta ggattccgcc ccttcccgca
     3901 aacgacccca aacaggaaac gcagctgaaa cgggaagctc aacacccact gacgcatggg
     3961 ttgttcaggc agtacttcat caaccagcaa ggcggcactt tcggccatcc gccgcgcccc
     4021 acagctcggg cagaaaccgc gacgcttaca gctgaaagcg accaggtgct cggcgtggca
     4081 agactcgcag cgaacccgta gaaagccatg ctccagccgc ccgcattgga gaaattcttc
     4141 aaattcccgt tgcacatagc ccggcaattc ctttccctgc tctgccataa gcgcagcgaa
     4201 tgccgggtaa tactcgtcaa cgatctgata gagaagggtt tgctcgggtc ggtggctctg
     4261 gtaacgacca gtatcccgat cccggctggc cgtcctggcc gccacatgag gcatgttccg
     4321 cgtccttgca atactgtgtt tacatacagt ctatcgctta gcggaaagtt cttttaccct
     4381 cagccgaaat gcctgccgtt gctagacatt gccagccagt gcccgtcact cccactctta
     4441 cattgttcgc agtatctagg tacatgttat tgcgtgcacc ttcatgatgt ataatgttat
     4501 taatgacagc agaaagatcg acagaattaa tattcttcgt acctgggaca gttatcgggt
     4561 gtcggccagt gatgagacca aaggatgcag tataagatga accactacta ttatcatggc
     4621 caccattacc accaccgtta aaactacctc ccgaacttcc agagccgcta tgaccatcat
     4681 tgtaggggtc atttggtccc atgtgaccaa aaccaccgac agtaccgcta tttaactctg
     4741 tactcataaa attatcctct ttgactttta aaaacagcaa aaactgtatt tataacaatg
     4801 tgtttatata cagtattcga aatactacgc ccgtcaaaac cacaccgtca acaggttttt
     4861 tgtgtatttt tcacgcctca cgggcagcct cagacctttt gtgttaaaac agtaatatgt
     4921 tctgctgtta caatacggta cacacaggag acactgtcac ttttatggca tggcacacag
     4981 ttcaagagac actgaaatta acaggaaagt cgcgctcgca attatatcgt gacatggcta
     5041 aaggccttgt cagctatcgc acaggaaaaa atgatcgccg ggagttcgag accagtgaac
     5101 tgattcgggc ctatggcgag ctgcgcactt ctgagacacc aaaaaggcac aaggagggac
     5161 actccaaatc tgtcgttagc caggcggaaa atgctcagct agacgtcatt cagcagaaac
     5221 tggacgctct gcagcagact gttaccctaa tgcttgaaga caagatggcg cgggaagcag
     5281 aaaaccgcct gcgaaatgag gagagggaac aactacaggc cgaaattagc cgtctggctg
     5341 acgcgctggc acaggaaaaa aagcggggat tctggtcgcg gctgtttcgc tccggttcag
     5401 agtaacagtg caggtgactc tgatttacca taacatctgt tatcagcacc gcctgagagg
     5461 ccctcagggc gtttctgacg ggaaaccggt aaaacaggca acaaaccacc cgaaaacccg
     5521 ccagaaacgc gcaggctctg tttttacggc atgcatgagt atgcgcacaa gtgcatagcg
     5581 cggaaattca caccattcag ccggggtaag tcctgtttcc ggcattcttc ccatccgaaa
     5641 acggtgaacg gggacgcaat ttattgcgtg gccgtgaagc gttgccggcg cagccggaaa
     5701 acccgctgtc agcacgttca ggaagggggt gatgtacaaa tggcacagag gatgaaagtg
     5761 acgtatgagc tgaaggaatg atgtaaaagt gatgtataag tgatgtaaaa gtgatgta
//
LOCUS       DAJTRD010000370         2761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.guided.227, whole genome shotgun sequence.
ACCESSION   DAJTRD010000370 DAJTRD010000000
VERSION     DAJTRD010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.guided.227"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..697)
                     /gene="intI1"
                     /locus_tag="OO315_004448"
     CDS             complement(<1..697)
                     /gene="intI1"
                     /locus_tag="OO315_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5893055.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWV"
     gene            907..1737
                     /locus_tag="OO315_004449"
     CDS             907..1737
                     /locus_tag="OO315_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5893056.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1850..2641
                     /gene="aadA1"
                     /locus_tag="OO315_004450"
     CDS             1850..2641
                     /gene="aadA1"
                     /locus_tag="OO315_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5893057.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          787 a          682 c          666 g          626 t
ORIGIN      
        1 aaacccagaa ccacggccag gaatgcccgg cgcgcggata cttccgctca agggcgtcgg
       61 gaagcgcaac gccgctgcgg ccctcggcct ggtccttcag ccaccatgcc cgtgcacgcg
      121 acagctgctc gcgcaggctg ggtgccaagc tctcgggtaa catcaaggcc cgatccttgg
      181 agcccttgcc ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc ttgacccgca
      241 gttgcaaacc ctcactgatc cgcatgcccg ttccatacag aagctgggcg aacaaacgat
      301 gctcgccttc cagaaaaccg aggatgcgaa ccacttcatc cggggtcagc accaccggca
      361 agcgccgcga cggccgaggt cttccgatct cctgaagcca gggcagatcc gtgcacagca
      421 ccttgccgta gaagaacagc aaggccgcca atgcctgacg atgcgtggag accgaaacct
      481 tgcgctcgtt cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg
      541 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
      601 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
      661 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
      721 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
      781 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttgg
      841 gcgaacccgg agcctcatta attgttagcc gttaaaatta agccctttac caaaccaata
      901 cttattatga aaaacacaat acatatcaac ttcgctattt ttttaataat tgcaaatatt
      961 atctacagca gcgccagtgc atcaacagat atctctactg ttgcatctcc attatttgaa
     1021 ggaactgaag gttgtttttt actttacgat gcatccacaa acgctgaaat tgctcaattc
     1081 aataaagcaa agtgtgcaac gcaaatggca ccagattcaa ctttcaagat cgcattatca
     1141 cttatggcat ttgatgcgga aataatagat cagaaaacca tattcaaatg ggataaaacc
     1201 cccaaaggaa tggagatctg gaacagcaat catacaccaa agacgtggat gcaattttct
     1261 gttgtttggg tttcgcaaga aataacccaa aaaattggat taaataaaat caagaattat
     1321 ctcaaagatt ttgattatgg aaatcaagac ttctctggag ataaagaaag aaacaacgga
     1381 ttaacagaag catggctcga aagtagctta aaaatttcac cagaagaaca aattcaattc
     1441 ctgcgtaaaa ttattaatca caatctccca gttaaaaact cagccataga aaacaccata
     1501 gagaacatgt atctacaaga tctggataat agtacaaaac tgtatgggaa aactggtgca
     1561 ggattcacag caaatagaac cttacaaaac ggatggtttg aagggtttat tataagcaaa
     1621 tcaggacata aatatgtttt tgtgtccgca cttacaggaa acttggggtc gaatttaaca
     1681 tcaagcataa aagccaagaa aaatgcgatc accattctaa acacactaaa tttataaaaa
     1741 atctaatggc aaaatcgccc aacccttcaa tcaagtcggg acggccaaaa gcaagctttt
     1801 ggctcccctc gctggcgctc ggcgcccctt atttcaaacg ttaaacatca tgagggaagt
     1861 ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg agcgccatct
     1921 cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg gcctgaagcc
     1981 acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa caacgcggcg
     2041 agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg agattctccg
     2101 cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa
     2161 gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta tcttcgagcc
     2221 agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac atagcgttgc
     2281 cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg atctatttga
     2341 ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg gcgatgagcg
     2401 aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc
     2461 gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc agcccgtcat
     2521 acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct cgcgcgcaga
     2581 tcagttggaa gaatttgttc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     2641 atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     2701 atgcactaag cacataattg cggtgatgct gccaacttac tgatttagtg tatgatggtg
     2761 t
//
LOCUS       DAJTRD010000371         2185 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.guided.240, whole genome shotgun sequence.
ACCESSION   DAJTRD010000371 DAJTRD010000000
VERSION     DAJTRD010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.guided.240"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            43..438
                     /gene="frdC"
                     /locus_tag="OO315_004451"
     CDS             43..438
                     /gene="frdC"
                     /locus_tag="OO315_004451"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5893058.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            449..808
                     /gene="frdD"
                     /locus_tag="OO315_004452"
     CDS             449..808
                     /gene="frdD"
                     /locus_tag="OO315_004452"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5893059.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            871..2004
                     /locus_tag="OO315_004453"
     CDS             871..2004
                     /locus_tag="OO315_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5893060.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
BASE COUNT          524 a          562 c          557 g          542 t
ORIGIN      
        1 actttcttat cgcgaccctg aaaccacgct aaggagtgca acatgacgac taaacgtaaa
       61 ccgtatgtac ggccaatgac gtccacctgg tggaaaaaat tgccgtttta tcgcttttac
      121 atgctgcgcg aaggcacggc ggttccggct gtgtggttca gcattgaact gattttcgcg
      181 ctgtttgccc tgaaaaatgg cccggaagcc tgggcgggat tcgtcgactt tttacaaaac
      241 ccggttatcg tgatcattaa cctgatcact ctgacggcag ctctgctgca caccaaaacc
      301 tggtttgaac tggcaccgaa agcggccaat atcattgtaa aagacgaaaa aatgggacca
      361 gagccaatta tcaaaagtct ctgggcggta actgtggttg ccaccatcgt aatcctgttt
      421 gttgccctgt actggtaagg agcctgagat gattaatcca aatccaaagc gttctgacga
      481 accggtattc tggggcctct tcggggccgg tggtatgtgg agcgcaatta ttgcgccggt
      541 gatgatcctg ctggtgggta ttctgctgcc actggggctg tttccgggtg atgcgctgag
      601 ctacgagcgc gttctggcgt tcgcgcagag cttcattggt cgcgtattcc tgttcctgat
      661 gatcgttctg ccgctgtggt gtggtttaca ccgtatgcac cacgcgatgc acgatctgaa
      721 aatccacgtg cctgcgggca aatgggtttt ctacggtctg gctgctatcc tgacagttgt
      781 cacgctgatt ggtgtcgtta caatctaacg catcgccaat gtaaatccgg cccgcctatg
      841 gcgggccgtt ttgtatggaa accagacctt atgttcaaaa cgacgctctg cgccttatta
      901 attaccgcct cttactccac atttgctgcc cctcaacaaa tcaacgatat tgtgcatcgc
      961 acaattaccc cgcttataga gcaacaaaag atccccggta tggcggtggc ggtaatttat
     1021 cagggtaaac cttattactt tacctggggc tatgcggaca tcgccaaaaa gcagcccgtc
     1081 acacagcaaa cgttgtttga gttaggttcg gtcagcaaaa catttacggg cgtgcttggt
     1141 ggcgacgcta ttgctcgagg ggaaatcaag ttaagcgatc ccgcaacaaa atactggcct
     1201 gaacttaccg ctaaacagtg gaatgggatc acactattac atcttgcgac ctacaccgct
     1261 ggcggcctgc cattgcaggt gccggatgaa gtgaaatcct caagcgactt gctgcgcttc
     1321 tatcaaaact ggcagcctgc atgggctcca ggaacacaac gtctgtatgc caactccagt
     1381 atcggtttgt tcggcgcact ggctgtgaag ccgtctggtt tgagttttga gcaggcgatg
     1441 caaactcgtg tcttccagcc actcaaactc aaccatacgt ggattaatgt acctccccca
     1501 gaagaaaaga attacgcctg gggatatcgc gaaggtaagg cagtgcatgt ttcgccaggg
     1561 gcgttagatg ctgaagctta tggtgtgaag tcgaccattg aagatatggc ccgctgggtg
     1621 caaagcaatt taccccccct tgatatcaat gagaaaacgc ttcaacaagg gatacaactg
     1681 gcacaatctc gctactggca aaccggcgat atgtatcagg gcctgggctg ggaaatgctg
     1741 gactggccgg tcaatcctga cagcatcatt aacggcagtg acaataaaat tgcactggca
     1801 gcacgcccgg taaaagcgat taagccccca actcctgcag tacgcgcatc atgggtacat
     1861 aaaacggggg cgaccggcgg atttggtagc tatgtcgcgt ttattccaga aaaagagctg
     1921 ggtatcgtga tgctggctaa caaaaactat cccaatccag cgagagtcgc cgccgcctgt
     1981 cagattctca acgctctaca gtaaaatttc atcgggtccg aattttcgga ccttttctgc
     2041 gcttttcctt gctgtcatct acacttagaa aaaaaccagt aaggaaacat ttatgcgcct
     2101 gctccctctc gttgccgcag cgacagctgc atttctggtc gttgcctgta gttttcctac
     2161 gccgccgcgt ggcgtgaccg tagtg
//
LOCUS       DAJTRD010000372         1878 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.guided.257, whole genome shotgun sequence.
ACCESSION   DAJTRD010000372 DAJTRD010000000
VERSION     DAJTRD010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.guided.257"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            complement(<1..337)
                     /locus_tag="OO315_004454"
     CDS             complement(<1..337)
                     /locus_tag="OO315_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="catalyzes the hydrolysis of Xaa-His dipeptides;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5893061.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTL"
     gene            599..1057
                     /gene="gpt"
                     /locus_tag="OO315_004455"
     CDS             599..1057
                     /gene="gpt"
                     /locus_tag="OO315_004455"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5893062.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1149..>1878
                     /locus_tag="OO315_004456"
     CDS             1149..>1878
                     /locus_tag="OO315_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5893063.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQ"
BASE COUNT          452 a          469 c          491 g          466 t
ORIGIN      
        1 ccagcgtggt gccgcgcgct ttaacccatt cgccatcaat ataaggctgg ataggatctt
       61 tcgtgaagtc atgcacggtg tcgttatttt tctgcggcac catatcgagg tgggcctgta
      121 agacgaccgg tttacgattt tccatacctg cggtagcagg ttgacgaatc aggatattac
      181 ctacctgatc gcgttcgaca tggaaacctt tctcttttgc ccaaccaaca atgtattcag
      241 cgagttgctc ttcatgatag gacgggtgag gaatagaaca gattttggca aaaatatccc
      301 acagcggctg tggagataat tgagacagtt cagacacgtt aagtctcctt gtcgatcacc
      361 cgcaaaacag tattgcaggt cacagggtta gcagaaaatg ttgtcaacac aagacaggct
      421 tgcgagatat gtattgagaa taccacttta tcccgcgtca gggagaggca gtgcgtaaaa
      481 agacgcggac tcatgtgaaa tactggtttt tagtgcgcca gatctctata atctcgcgca
      541 acctattttc ccctcgaaca ctttttaagc cgtagataaa caggctggga cacttcacat
      601 gagcgaaaaa tacatcgtca cctgggacat gttgcagatc catgcacgta aactcgcaag
      661 ccgactgatg ccttctgaac aatggaaagg cattattgcc gtaagccgtg gcggtctggt
      721 accgggggcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
      781 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
      841 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgctg ttgcgattcg
      901 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
      961 ggttgatgac tatgttgttg atatcccgca agatacctgg atcgaacagc cgtgggatat
     1021 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcattgcc
     1081 gggcgttgtt ctttttaact tcaggcaggt tacaatagtt tccagtaagt attctggagg
     1141 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
     1201 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
     1261 ctcttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     1321 ggcgcggtat tgacccacgt gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
     1381 ctgaacgtac cgacgatgat ttatacgata ccgtgattgg ctaccgtggt ggcaactgga
     1441 tttatgagtg ggccacccag gcgatggtgt ggcaacaaaa agcctgtacg gaagaagatc
     1501 cgcaactcag tggtcgacac tggctgcatg cggctacgtt gtacaacatt gccgcctatc
     1561 ctcatctgaa aggagatgac ctggccgagc aagcgcaggc tttatcaaac cgcgcctatg
     1621 aagaggccgc ccagcgtctg ccgggcacga tgcggcagat ggagtttacc gtacccggcg
     1681 gtgcgcccat caccggcttt ttgcatatgc cgaaaggcga tggcccgttc ccgacagtat
     1741 taatgtgtgg tggtctggat gcgatgcaga cggactatta cagcctgtat gaacgttatt
     1801 ttgcgccgcg cggcattgcg atgctgacta ttgatatgcc gtcggtgggc ttttcttcaa
     1861 aatggaagct cacccagg
//
LOCUS       DAJTRD010000373         1761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.guided.263, whole genome shotgun sequence.
ACCESSION   DAJTRD010000373 DAJTRD010000000
VERSION     DAJTRD010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.guided.263"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            48..1253
                     /gene="tet(B)"
                     /locus_tag="OO315_004457"
                     /pseudo
     CDS             48..1253
                     /gene="tet(B)"
                     /locus_tag="OO315_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
     gene            complement(1366..>1761)
                     /locus_tag="OO315_004458"
     CDS             complement(1366..>1761)
                     /locus_tag="OO315_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5893064.1"
                     /translation="QQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIIDNNKNLIPI
                     QEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWFMHIAI
                     HAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          450 a          318 c          385 g          608 t
ORIGIN      
        1 tgatagagtt attttaccac tccctatcag tgatagagaa aagtgaaatg aatagttcga
       61 caaagatcgc attggtaatt acgttactcg atgccatggg gattggcctt atcatgccag
      121 tcttgccaac gttattacgt gaatttattg cttcggaaga tatcgctaac cactttggcg
      181 tattgcttgc actttatgcg ttaatgcagg ttatctttgc tccttggctt ggaaaaatgt
      241 ctgaccgatt tggtcggcgc ccagtgctgt tgttgtcatt aataggcgca tcgctggatt
      301 acttattgct ggctttttca agtgcgcttt ggatgctgta tttaggccgt ttgctttcag
      361 ggatcacagg agctactggg gctgtcgcgg catcggtcat tgccgatacc acctcagctt
      421 ctcaacgcgt gaagtggttc ggttggttag gggcaagttt tgggcttggt ttaatagcgg
      481 ggcctattat tggtggtttt gcaggagaga tttcaccgca tagtcccttt tttatcgctg
      541 cgttgctaaa tattgtcgct ttccttgtgg ttatgttttg gttccgtgaa accaaaaata
      601 cacgtgataa tacagatacc gaagtagggg ttgagacgca atcgaattcg gtatacatca
      661 ctttatttaa aacgatgccc attttgttga ttatttattt ttcagcgcaa ttgataggcc
      721 aaattcccgc aacggtgtgg gtgctattta ccgaaaatcg ttttggatgg aatagcatga
      781 tggttggctt ttcattagcg ggtcttggtc ttttacactc agtattccaa gcctttgtgg
      841 caggaagaat agccactaaa tggggcgaaa aaacggcagt actgctcgga tttattgcag
      901 atagtagtgc atttgccttt ttagcgttta tatctgaagg ttggttagtt ttccctgttt
      961 taattttatt ggctggtggt gggatcgctt tacctgcatt acagggagtg atgtctatcc
     1021 aaacaaagag tcatcagcaa ggtgctttac agggattatt ggtgagcctt accaatgcaa
     1081 ccggtgttat tggcccatta ctgtttgctg ttatttataa tcattcacta ccaatttggg
     1141 atggctagat ttggattatt ggtttagcgt tttactgtat tattatcctg ctatcgatga
     1201 ccttcatgtt aacccctcaa gctcagggga gtaaacagga gacaagtgct tagttatttc
     1261 gtcaccaaat gatgttattc cgcgaaatat aatgaccctc ttgataaccc aagagggcat
     1321 tttttacgat aaagaagatt tagcttcaaa taaaacctat ctattttatt tatctttcaa
     1381 gctcaataaa aagccgcggt aaatagcaat aaattggcct tttttatcgg caagctcttt
     1441 taggtttttc gcatgtattg cgatatgcat aaaccagcca ttgagtaagt ttttaagcac
     1501 atcatcatca taagctttaa gttggttctc ttggatcaat ttgctgacaa tggcgtttac
     1561 cttaccagta atgtattcaa ggctaatttt ttcaagttca ttccaaccaa tgataggcat
     1621 cacttcttgg atagggataa ggtttttatt attatcaata atataatcaa gataatgttc
     1681 aaatatactt tctaaggcag accaaccatt tgttaaatca gtttttgttg tgatgtaggc
     1741 atcaatcata attaattgct g
//
LOCUS       DAJTRD010000374         1761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.guided.264, whole genome shotgun sequence.
ACCESSION   DAJTRD010000374 DAJTRD010000000
VERSION     DAJTRD010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.guided.264"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            48..1253
                     /gene="tet(B)"
                     /locus_tag="OO315_004459"
     CDS             48..1253
                     /gene="tet(B)"
                     /locus_tag="OO315_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5893065.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(1366..>1761)
                     /locus_tag="OO315_004460"
     CDS             complement(1366..>1761)
                     /locus_tag="OO315_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5893066.1"
                     /translation="QQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIIDNNKNLIPI
                     QEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLNGWFMHIAI
                     HAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          449 a          318 c          386 g          608 t
ORIGIN      
        1 tgatagagtt attttaccac tccctatcag tgatagagaa aagtgaaatg aatagttcga
       61 caaagatcgc attggtaatt acgttactcg atgccatggg gattggcctt atcatgccag
      121 tcttgccaac gttattacgt gaatttattg cttcggaaga tatcgctaac cactttggcg
      181 tattgcttgc actttatgcg ttaatgcagg ttatctttgc tccttggctt ggaaaaatgt
      241 ctgaccgatt tggtcggcgc ccagtgctgt tgttgtcatt aataggcgca tcgctggatt
      301 acttattgct ggctttttca agtgcgcttt ggatgctgta tttaggccgt ttgctttcag
      361 ggatcacagg agctactggg gctgtcgcgg catcggtcat tgccgatacc acctcagctt
      421 ctcaacgcgt gaagtggttc ggttggttag gggcaagttt tgggcttggt ttaatagcgg
      481 ggcctattat tggtggtttt gcaggagaga tttcaccgca tagtcccttt tttatcgctg
      541 cgttgctaaa tattgtcgct ttccttgtgg ttatgttttg gttccgtgaa accaaaaata
      601 cacgtgataa tacagatacc gaagtagggg ttgagacgca atcgaattcg gtatacatca
      661 ctttatttaa aacgatgccc attttgttga ttatttattt ttcagcgcaa ttgataggcc
      721 aaattcccgc aacggtgtgg gtgctattta ccgaaaatcg ttttggatgg aatagcatga
      781 tggttggctt ttcattagcg ggtcttggtc ttttacactc agtattccaa gcctttgtgg
      841 caggaagaat agccactaaa tggggcgaaa aaacggcagt actgctcgga tttattgcag
      901 atagtagtgc atttgccttt ttagcgttta tatctgaagg ttggttagtt ttccctgttt
      961 taattttatt ggctggtggt gggatcgctt tacctgcatt acagggagtg atgtctatcc
     1021 aaacaaagag tcatcagcaa ggtgctttac agggattatt ggtgagcctt accaatgcaa
     1081 ccggtgttat tggcccatta ctgtttgctg ttatttataa tcattcacta ccaatttggg
     1141 atggctggat ttggattatt ggtttagcgt tttactgtat tattatcctg ctatcgatga
     1201 ccttcatgtt aacccctcaa gctcagggga gtaaacagga gacaagtgct tagttatttc
     1261 gtcaccaaat gatgttattc cgcgaaatat aatgaccctc ttgataaccc aagagggcat
     1321 tttttacgat aaagaagatt tagcttcaaa taaaacctat ctattttatt tatctttcaa
     1381 gctcaataaa aagccgcggt aaatagcaat aaattggcct tttttatcgg caagctcttt
     1441 taggtttttc gcatgtattg cgatatgcat aaaccagcca ttgagtaagt ttttaagcac
     1501 atcatcatca taagctttaa gttggttctc ttggatcaat ttgctgacaa tggcgtttac
     1561 cttaccagta atgtattcaa ggctaatttt ttcaagttca ttccaaccaa tgataggcat
     1621 cacttcttgg atagggataa ggtttttatt attatcaata atataatcaa gataatgttc
     1681 aaatatactt tctaaggcag accaaccatt tgttaaatca gtttttgttg tgatgtaggc
     1741 atcaatcata attaattgct g
//
LOCUS       DAJTRD010000375         1275 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 100855
            SAMEA8899803-rid15818773.guided.282, whole genome shotgun sequence.
ACCESSION   DAJTRD010000375 DAJTRD010000000
VERSION     DAJTRD010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8899803
            Sequence Read Archive: ERR6004878
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477792.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477792.1
            Genome Coverage       :: 55x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:29:03
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,461
            CDSs (total)                      :: 4,375
            Genes (coding)                    :: 3,985
            CDSs (with protein)               :: 3,985
            Genes (RNA)                       :: 86
            rRNAs                             :: 2, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 1 (5S, 16S, 23S)
            tRNAs                             :: 74
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 390
            CDSs (without protein)            :: 390
            Pseudo Genes (ambiguous residues) :: 0 of 390
            Pseudo Genes (frameshifted)       :: 183 of 390
            Pseudo Genes (incomplete)         :: 106 of 390
            Pseudo Genes (internal stop)      :: 147 of 390
            Pseudo Genes (multiple problems)  :: 45 of 390
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1275
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8899803-rid15818773.guided.282"
                     /serovar="S. flexneri 2a"
                     /isolate="100855"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Gambia"
                     /collection_date="2008-12-25"
     gene            263..922
                     /locus_tag="OO315_004461"
     CDS             263..922
                     /locus_tag="OO315_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5893067.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT          338 a          279 c          301 g          357 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttc
//
